thbs3a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	thbs3a
Entrez Gene ID	252849
Full Name	thrombospondin 3a
Synonyms	thbs3,tsp3,zgc:103461
General protein information	Preferred Names thrombospondin 3a Names thrombospondin-3a Tsp-3a
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_173225.1	NP_775332.1	thrombospondin-3a precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	THBS3	XP_001154764.1
Bos taurus (cattle)	THBS3	NP_001095309.1
Danio rerio (zebrafish)	thbs3a	NP_775332.1
Macaca mulatta (Rhesus monkey)	THBS3	XP_001115770.1
Mus musculus (house mouse)	Thbs3	NP_038719.2
Xenopus tropicalis (tropical clawed frog)	thbs3	NP_001011401.1
Homo sapiens (human)	THBS3	NP_009043.1
Canis lupus familiaris (dog)	THBS3	XP_003434941.1
Rattus norvegicus (Norway rat)	LOC681309	XP_003749368.1

Gene Ontology
Bibliography

Related articles in PubMed

Lin CY, He JY, Zeng CW, Loo MR, Chang WY, Zhang PH, Tsai HJ
Open biology7(7)(2017 07)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Phylogenomic analysis of vertebrate thrombospondins reveals fish-specific paralogues, ancestral gene relationships and a tetrapod innovation.
McKenzie P, Chadalavada SC, Bohrer J, Adams JC
BMC evolutionary biology633(2006 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following thbs3a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the thbs3a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa38685	NM_173225.1 Latest version!	Danio rerio thrombospondin 3a (thbs3a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

thbs3a ( NM_173225.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_775332.1 Danio rerio thrombospondin 3a (thbs3a), mRNA.

Price & Availability↑

CloneID	ODa38685
Clone ID Related Accession (Same CDS sequence)	NM_173225.1
Accession Version	NM_173225.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550160000000
Organism	Danio rerio(zebrafish)
Product	thrombospondin-3a precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF462310.1. ##Evidence-Data-START## Transcript exon combination :: AF462310.1, BC085524.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGAAACAAA TGTTTGTACA CATTTGGGTC AGTCTGGTCG TGTTGATGTT CGTCTGGTCA 
GCACAGAGCG ACAAGAAACA AGACGTGCCA GTGATAGACG TGTTGGGTTT GGAGGATGTG 
AAGCAGACCG TGGCCGCGGT GGAGAAGCTC TCGTTGGCTC TGAAAACTCT CAGTGACGTG 
TATGTGATGT CTACTTTTCG TCTTCCTCCA AAACTTGGTG GAGTTCTGCT GGGGCTCTAC 
AACAAACAGG ACAACAAGAA GTACCTGGAG GTTGCCATCA TGAGCAAGAT TAACAAAGTT 
CTGGTCAGGT ATGTCCGTGA GGATGGAAAG CTGCATACAG TGAACATGCA GAGCCCGAAT 
GTTGCGGATG GACGTCCTCA GTCACTGATT CTGCGTGTCG GGGGCCTGCG CCGGGAGTAT 
CTTAGCCTCG AGCTCTATGT GAACTGTCGT CTGGCTGACT CCGCCCAGCG CCTGCCCCCA 
TTGGTGGATT TGCCCAGAGA TGCTGAGCTG GTGGAGATAC GAAATGGACA CAAGGCATAT 
GCTCGGATGC AGGGTTCAAT GGATACTCTT AAACTGGCAC TGGGGGGCAC TGTTGCCCAG 
GCTGGCGCTC TCACCGACTG TCCCTTTCAG GGAGACGCTT CTTCATACAA CATAGTGAAT 
GGTGAGGTTA ATTCTATTCT AGGTGACCAC ACTAAAGCTC TGATCGGCCA GCTGATCATC 
TTTAATCAGA TCTTGGGAGA GTTGAGAGAG GACATCAGAG AACAGGTGAA AGAAATGTCC 
CTGGTAAGGA ACGCTATTCT GGAGTGCCAG ATGTGTGGGT TTCACGAGCC TCGTTCCCGC 
TGTCAGCCAA ATCCCTGTTT CAAGGGTGTG TCCTGCATGG AGACGTTTGA ATACCCAGGG 
TACCGCTGTG GGCCCTGTCC AGACGGCATG ACGGGCAACG GTACACACTG TCAGGACATC 
GATGAGTGTT CAGAGGCTCA GCCGTGTTAT ACACCGGGTG CTTGTGTCAA TACTGCAAGA 
GGTTTTACCT GTGAGTCATG TCCCCCTGGC ATGTGGGGTC CGCCTTTGTC TGGAGTGGGA 
GTGGAGTATG CTAAGAGTCA CCGTCAGGAG TGCTCTGACA TCGATGAATG TGTAGATTTG 
GCCAACGCCT GCACACCCAA CTCTGTCTGC ATCAACACTA TTGGGTCATT TCGATGCGGT 
CAGTGCAAGA CTGGATATGT TGGCAATCAG ACAGCCGGAT GTTTCCCTCG AAAATCATGT 
TCTTCACTCA GTTTCAACCC CTGTGACACG AACGCTCACT GCGTCATGCA ACGCAACGGG 
GACGTGTCCT GCGCTTGTAA TGTTGGATGG GCTGGTAATG GTCACACCTG CGGGAAGGAC 
ACAGATATTG ATGGTTATCC TGACCGCTCT CTGCCCTGCA TGGACAATCA CAAGCACTGC 
AGACAGGACA ACTGTGTCTA CACACCCAAT TCTGGTCAGG AAGATGCGGA TAATGATGGA 
ATTGGAGATC AGTGTGATGA AGATGCTGAT GGAGACGGAA TTAAGAACGT AGAGGATAAC 
TGTCGTTTGG TCTCCAATAA AGACCAGCAG AACTCGGACA CAGATTCGTT CGGAGACGCG 
TGTGATAACT GTCCCACCGT TCCCAACATC GACCAGAAAG ACACTGATAG CAACGGAGAG 
GGAGACGCTT GTGATGACGA CATTGATGGA GATGGGATTC AGAATGTTCT GGATAACTGT 
CCTAAAGTGC CGAACCCGAT GCAGACGGAC CGGGACAGAG ACGGAGTAGG CGATGCATGT 
GACAGCTGTC CTGAGATCAG CAACCCCATG CAGACGGATG TAGACAATGA CTTAGTCGGG 
GATGTTTGTG ACACCAACCA AGACACAGAT GGAGACGGAC ACCAGGACAC TCGGGATAAC 
TGTCCCGATA TCCCAAACAG CTCTCAGCTG GACTCAGATA ATGACGGCAT CGGAGACGAC 
TGTGATGAAG ACGACGACAA CGATGGCATC CCGGACAATC ACGCCATCAA TGGCATAGGG 
CCCGATAACT GCAGACTCAT ATCCAACCCC AACCAGAAAG ACTCAGACAG TAATGGAGTG 
GGCGATGTAT GCGAGAATGA CTTCGACAAT GATTCAGTGA TGGATTTGGT TGACGTGTGT 
CCAGAAAGTG CTGAAGTCAC ACTTACAGAT TTCAGAGCCT ATCAAACGGT CATTCTGGAC 
CCGGAAGGTG ACGCTCAGAT TGACCCCAAC TGGGTGGTGC TCAATCAGGG AATGGAAATA 
GTTCAGACCA TGAACAGTGA CCCTGGACTG GCAGTGGGTT ATACAGCGTT TAACGGCGTG 
GACTTTGAGG GAACATTTCA CGTGAACACA GTGACCGACG ATGACTATGC CGGCTTCATC 
TTCGGCTACC AGGATTCATC TAGTTTCTAC GTGGTGATGT GGAAGCAGAC CGAACAGACA 
TACTGGCAGT CCATCCCATT CAGAGCCATG GCTGAACCGG GCCTGCAGCT CAAGGCAGTG 
AAGTCTCGTA CTGGTCCTGG TGAGTTTCTT CGTAACGCTC TGTGGCATGC TGGAGATACT 
GATGGAGAGG TGAAGCTGCT GTGGAAAGAC CCACGAAACG TCGGCTGGCT GGATAAAACA 
TCGTATCGGT GGCAGCTCAG CCATCGGCCT CAGGTCGGAT ACATCAGGGT GAAGCTGTAT 
GAGGGCTCAG AGATGGTGGC AGACTCAGAT GTGGTAATCG ACACCTCTAT GAGAGGAGGT 
CGTCTGGGCG TCTTCTGCTT CTCTCAGGAG AACATCATTT GGTCCAATCT GCGCTACCGC 
TGCAACGATA CCGTTCCAGA GGACTTCAGT TCACACCGTA AGCAGGTTCT GATGCACATA 
AAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775332.1
CDS	85..2973
Translation	MKQMFVHIWVSLVVLMFVWSAQSDKKQDVPVIDVLGLEDVKQTVAAVEKLSLALKTLSDVYVMSTFRLPPKLGGVLLGLYNKQDNKKYLEVAIMSKINKVLVRYVREDGKLHTVNMQSPNVADGRPQSLILRVGGLRREYLSLELYVNCRLADSAQRLPPLVDLPRDAELVEIRNGHKAYARMQGSMDTLKLALGGTVAQAGALTDCPFQGDASSYNIVNGEVNSILGDHTKALIGQLIIFNQILGELREDIREQVKEMSLVRNAILECQMCGFHEPRSRCQPNPCFKGVSCMETFEYPGYRCGPCPDGMTGNGTHCQDIDECSEAQPCYTPGACVNTARGFTCESCPPGMWGPPLSGVGVEYAKSHRQECSDIDECVDLANACTPNSVCINTIGSFRCGQCKTGYVGNQTAGCFPRKSCSSLSFNPCDTNAHCVMQRNGDVSCACNVGWAGNGHTCGKDTDIDGYPDRSLPCMDNHKHCRQDNCVYTPNSGQEDADNDGIGDQCDEDADGDGIKNVEDNCRLVSNKDQQNSDTDSFGDACDNCPTVPNIDQKDTDSNGEGDACDDDIDGDGIQNVLDNCPKVPNPMQTDRDRDGVGDACDSCPEISNPMQTDVDNDLVGDVCDTNQDTDGDGHQDTRDNCPDIPNSSQLDSDNDGIGDDCDEDDDNDGIPDNHAINGIGPDNCRLISNPNQKDSDSNGVGDVCENDFDNDSVMDLVDVCPESAEVTLTDFRAYQTVILDPEGDAQIDPNWVVLNQGMEIVQTMNSDPGLAVGYTAFNGVDFEGTFHVNTVTDDDYAGFIFGYQDSSSFYVVMWKQTEQTYWQSIPFRAMAEPGLQLKAVKSRTGPGEFLRNALWHAGDTDGEVKLLWKDPRNVGWLDKTSYRWQLSHRPQVGYIRVKLYEGSEMVADSDVVIDTSMRGGRLGVFCFSQENIIWSNLRYRCNDTVPEDFSSHRKQVLMHIKV

Target ORF information:

RefSeq Version	NM_173225.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio thrombospondin 3a (thbs3a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173225.1

ORF Insert Sequence:

ATGAAACAAA TGTTTGTACA CATTTGGGTC AGTCTGGTCG TGTTGATGTT CGTCTGGTCA 
GCACAGAGCG ACAAGAAACA AGACGTGCCA GTGATAGACG TGTTGGGTTT GGAGGATGTG 
AAGCAGACCG TGGCCGCGGT GGAGAAGCTC TCGTTGGCTC TGAAAACTCT CAGTGACGTG 
TATGTGATGT CTACTTTTCG TCTTCCTCCA AAACTTGGTG GAGTTCTGCT GGGGCTCTAC 
AACAAACAGG ACAACAAGAA GTACCTGGAG GTTGCCATCA TGAGCAAGAT TAACAAAGTT 
CTGGTCAGGT ATGTCCGTGA GGATGGAAAG CTGCATACAG TGAACATGCA GAGCCCGAAT 
GTTGCGGATG GACGTCCTCA GTCACTGATT CTGCGTGTCG GGGGCCTGCG CCGGGAGTAT 
CTTAGCCTCG AGCTCTATGT GAACTGTCGT CTGGCTGACT CCGCCCAGCG CCTGCCCCCA 
TTGGTGGATT TGCCCAGAGA TGCTGAGCTG GTGGAGATAC GAAATGGACA CAAGGCATAT 
GCTCGGATGC AGGGTTCAAT GGATACTCTT AAACTGGCAC TGGGGGGCAC TGTTGCCCAG 
GCTGGCGCTC TCACCGACTG TCCCTTTCAG GGAGACGCTT CTTCATACAA CATAGTGAAT 
GGTGAGGTTA ATTCTATTCT AGGTGACCAC ACTAAAGCTC TGATCGGCCA GCTGATCATC 
TTTAATCAGA TCTTGGGAGA GTTGAGAGAG GACATCAGAG AACAGGTGAA AGAAATGTCC 
CTGGTAAGGA ACGCTATTCT GGAGTGCCAG ATGTGTGGGT TTCACGAGCC TCGTTCCCGC 
TGTCAGCCAA ATCCCTGTTT CAAGGGTGTG TCCTGCATGG AGACGTTTGA ATACCCAGGG 
TACCGCTGTG GGCCCTGTCC AGACGGCATG ACGGGCAACG GTACACACTG TCAGGACATC 
GATGAGTGTT CAGAGGCTCA GCCGTGTTAT ACACCGGGTG CTTGTGTCAA TACTGCAAGA 
GGTTTTACCT GTGAGTCATG TCCCCCTGGC ATGTGGGGTC CGCCTTTGTC TGGAGTGGGA 
GTGGAGTATG CTAAGAGTCA CCGTCAGGAG TGCTCTGACA TCGATGAATG TGTAGATTTG 
GCCAACGCCT GCACACCCAA CTCTGTCTGC ATCAACACTA TTGGGTCATT TCGATGCGGT 
CAGTGCAAGA CTGGATATGT TGGCAATCAG ACAGCCGGAT GTTTCCCTCG AAAATCATGT 
TCTTCACTCA GTTTCAACCC CTGTGACACG AACGCTCACT GCGTCATGCA ACGCAACGGG 
GACGTGTCCT GCGCTTGTAA TGTTGGATGG GCTGGTAATG GTCACACCTG CGGGAAGGAC 
ACAGATATTG ATGGTTATCC TGACCGCTCT CTGCCCTGCA TGGACAATCA CAAGCACTGC 
AGACAGGACA ACTGTGTCTA CACACCCAAT TCTGGTCAGG AAGATGCGGA TAATGATGGA 
ATTGGAGATC AGTGTGATGA AGATGCTGAT GGAGACGGAA TTAAGAACGT AGAGGATAAC 
TGTCGTTTGG TCTCCAATAA AGACCAGCAG AACTCGGACA CAGATTCGTT CGGAGACGCG 
TGTGATAACT GTCCCACCGT TCCCAACATC GACCAGAAAG ACACTGATAG CAACGGAGAG 
GGAGACGCTT GTGATGACGA CATTGATGGA GATGGGATTC AGAATGTTCT GGATAACTGT 
CCTAAAGTGC CGAACCCGAT GCAGACGGAC CGGGACAGAG ACGGAGTAGG CGATGCATGT 
GACAGCTGTC CTGAGATCAG CAACCCCATG CAGACGGATG TAGACAATGA CTTAGTCGGG 
GATGTTTGTG ACACCAACCA AGACACAGAT GGAGACGGAC ACCAGGACAC TCGGGATAAC 
TGTCCCGATA TCCCAAACAG CTCTCAGCTG GACTCAGATA ATGACGGCAT CGGAGACGAC 
TGTGATGAAG ACGACGACAA CGATGGCATC CCGGACAATC ACGCCATCAA TGGCATAGGG 
CCCGATAACT GCAGACTCAT ATCCAACCCC AACCAGAAAG ACTCAGACAG TAATGGAGTG 
GGCGATGTAT GCGAGAATGA CTTCGACAAT GATTCAGTGA TGGATTTGGT TGACGTGTGT 
CCAGAAAGTG CTGAAGTCAC ACTTACAGAT TTCAGAGCCT ATCAAACGGT CATTCTGGAC 
CCGGAAGGTG ACGCTCAGAT TGACCCCAAC TGGGTGGTGC TCAATCAGGG AATGGAAATA 
GTTCAGACCA TGAACAGTGA CCCTGGACTG GCAGTGGGTT ATACAGCGTT TAACGGCGTG 
GACTTTGAGG GAACATTTCA CGTGAACACA GTGACCGACG ATGACTATGC CGGCTTCATC 
TTCGGCTACC AGGATTCATC TAGTTTCTAC GTGGTGATGT GGAAGCAGAC CGAACAGACA 
TACTGGCAGT CCATCCCATT CAGAGCCATG GCTGAACCGG GCCTGCAGCT CAAGGCAGTG 
AAGTCTCGTA CTGGTCCTGG TGAGTTTCTT CGTAACGCTC TGTGGCATGC TGGAGATACT 
GATGGAGAGG TGAAGCTGCT GTGGAAAGAC CCACGAAACG TCGGCTGGCT GGATAAAACA 
TCGTATCGGT GGCAGCTCAG CCATCGGCCT CAGGTCGGAT ACATCAGGGT GAAGCTGTAT 
GAGGGCTCAG AGATGGTGGC AGACTCAGAT GTGGTAATCG ACACCTCTAT GAGAGGAGGT 
CGTCTGGGCG TCTTCTGCTT CTCTCAGGAG AACATCATTT GGTCCAATCT GCGCTACCGC 
TGCAACGATA CCGTTCCAGA GGACTTCAGT TCACACCGTA AGCAGGTTCT GATGCACATA 
AAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Open biology7(7)(2017 07)
Lin CY,He JY,Zeng CW,Loo MR,Chang WY,Zhang PH,Tsai HJ

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Phylogenomic analysis of vertebrate thrombospondins reveals fish-specific paralogues, ancestral gene relationships and a tetrapod innovation.
BMC evolutionary biology633(2006 Apr)
McKenzie P,Chadalavada SC,Bohrer J,Adams JC

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thbs3a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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