HEPHL1B cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	HEPHL1B
Entrez Gene ID	101886332
Full Name	hephaestin-like 1b
Synonyms	si:ch73-60g14.1,si:ch73-60g14.3,si:ch73-60g14.4
General protein information	Preferred Names hephaestin-like 1b Names LOW QUALITY PROTEIN: hephaestin-like protein 1 hephaestin-like protein 1
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021472277.1	XP_021327952.1	hephaestin-like protein 1 isoform X2
XM_021479509.1	XP_021335184.1	LOW QUALITY PROTEIN: hephaestin-like protein 1 isoform X1
XM_017358041.1	XP_017213530.1	LOW QUALITY PROTEIN: hephaestin-like protein 1

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	hephl1	XP_002939767.2
Homo sapiens (human)	HEPHL1	NP_001092142.1
Macaca mulatta (Rhesus monkey)	HEPHL1	XP_001086917.1
Gallus gallus (chicken)	HEPHL1	XP_417192.4
Danio rerio (zebrafish)	LOC101886332	XP_005155347.1
Pan troglodytes (chimpanzee)	HEPHL1	XP_508699.2
Canis lupus familiaris (dog)	HEPHL1	XP_542245.3
Bos taurus (cattle)	HEPHL1	NP_001179440.1
Mus musculus (house mouse)	Hephl1	NP_001158269.1
Rattus norvegicus (Norway rat)	Hephl1	NP_001178922.1

Gene Ontology

The following HEPHL1B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HEPHL1B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa145114	XM_021472277.1 Latest version!	Danio rerio hephaestin-like 1b (hephl1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa147815	XM_021479509.1 Latest version!	Danio rerio hephaestin-like 1b (hephl1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa54095	XM_017358041.1 Latest version!	Danio rerio hephaestin-like 1b (hephl1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HEPHL1B ( XM_021472277.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021327952.1 Danio rerio hephaestin-like 1b (hephl1b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa145114
Clone ID Related Accession (Same CDS sequence)	XM_021472277.1
Accession Version	XM_021472277.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	hephaestin-like protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394785.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTCAA TTATGGAAGA CAATTACCCG TTTAAGCTAA TAATTCTTCA GAAAACTCTA 
CTTACTTTCT TTTTCCTCAG GCAGGCATCA GTATTTCTGC AACGTGGGGC TCGGCGGATT 
GGCAGTGTGT ATAAAAAGGC AATTTACAGG CAGTACACAG ATTCTTCTTA CTCTCAGGAG 
GTCCCCAAAC CCCCCTGGCT GGGTTACCTG GGGCCCGTCA TGAGGGCTGA GGAGCAGGAT 
GTTATCATAG TGCACATGAA GAACTTTGGC TCCAGACGTT ACTCCATGCA TCCACATGGC 
GTCTTCTATG CCAAAGACTC AGAAGGTGCG TTGTATCCAG ACGGAACCTC AGACAGTCTG 
AAGCAAGATG ACGGTGTTCC TCCAGGGGGA AACTACACCT ACACCTGGAT TGTGAAAGCA 
GAATCTGCCC CTCAGAAGGA TGACCCAAAC TGCCTGACAT GGGCCTACCA TTCACACGTC 
AGTGCTTCAC AGGACATCTC CTCTGGCCTT ATTGGAGCTC TTCTCACCTG CAAGAAAGGA 
ACCCTGTGTG AGACGTCCAC TTCCGATCAG CACCACGCTC ATCGTGAGGA TGTGGACCAG 
GATCTCATCC TCATGTTCAG TGTGGTGGAT GAGAACATCA GCTGGTATCT AAAAGAGAAC 
ATCCAGAGCT TCTGCTCCGA CCCAGATGGT GTGGATCCTG CAAGTGAAGA CTTCCAAGAA 
TCCAATTTGA TGCACGCCAT TAATGGATAT GTGTATGGAA ACCTACCGGG GATTGAGTTT 
TGTCAAAACC GCACAGTTGC TCTGCATCTC TTTGGCATGG GCAATGAGAT CGACATTCAC 
TCTGTTCATT TCCACGGGCA CACACTTCTG GACCGCGGGC ACCGTGTGGA TGTCATGAGT 
CTGTTTTCTG CCACTTTTGC CACTGCAGAC ATGATTCCTG CCACAATAGG GACGTGGCTG 
TTAAACTGCC AGGTCAATGA TCATCTACAA GCTGGTATGC AGGCACTGTT CAATGTGTCG 
TCCTGCAGTG ATGAGGCGAG CTCCACGGCT CCTCTCAGTG GGAAGATCAG GAAGTATTTC 
ATTGCTGCTG AGAAGGTTCA GTGGGATTAC GGACCATCAG GGATGAACCA AGTGACCAAG 
GAGTCTCTAA TCGATCCTGG AAGCTTTTCA GAGGTTTTCT TTGGGACCAA TAATAGCCGA 
ATTGGTGGAA AGTACTGGAA GGTGATATAC AGAGAATATA CAGATAAGAC TTTCACCTTA 
AAGAGAAAGC AAACCAGTGC TCAGACTCAT CTAGGCATTC TGGGTCCAGT ACTGAGGGCA 
GAGGTTGGAG ATACACTTCA GGTGATGTTC CTGAATAAAG CTGACAGAAA CTACAGTATC 
CAGCCTCATG GACTCCAGTA CGACAAACCC TTCGAGGGAG TATTTTATCA AGATGGTATA 
AAGAGAAAAG GATATGAAGT TCAGCCAGGT GAAACGTTCA CCTACATGTG GAAGTTGAGG 
GAAGGACCGT CTGAAAGTGA CCCTCCCTGT ATTTCCTACC TGTACTTTTC CTCCAGTAAT 
GCAGTCAGAG ACACTAACTC TGGCCTCGCA GGCCCCCTGC TGGTGTGTAA GAAAGATGCG 
CTGACATCCA GTGGCACTCA GAAAGAGGTG GATCATGAGT TTTTCCTGCT TTTTACTGTG 
TTCAATGAAA ACCTCAGCTG GTACTTGGAT CAGAACATTG AAACCTTTGG CACCAATGAG 
TCAGACCCAG AAAATCTGGA CTTTCAGGAG AGCAATAAAA TGCATGCTGT GAATGGCTTC 
ATGTATGGGA ACCTACCTGG CCTTGAGATG AATAAGGGGT CCAGGGTCAG ATGGCATCTT 
TTAGGACTTG GCACAGAGAT GGACATTCAT GGCATTTACT TCCAAGGCAA CACCTTCGAG 
AAACAGGGCA CAACACATGA CACTCTGAGC CTGTTTCCAC ACACTGCAGT CACTGTTTTC 
ATGCAACCAG ATGTCAGTGG TGTATTTGAG CTGAGCTGCC TAGTGTGGGA TCACTATGAT 
AGTGGGATGA GACAACTGTA TGAAGTGACA GGACCAAGAT ATGAAGAGCC CTCAGTCTCA 
CCATCTGAGA CAGTGGAGTA TTTCATCAGT GCTGAAGAGC TGGAGTGGGA CTATTCGCCT 
GATCGCACAT GGGAACTGCA GAACTTCAAC ACAACGGAGG ATGAAAGGCA GGTCCATGTG 
CTACATGCAG CGTTTGTTGG TATTGCAGTG TTTGAATGGC TTCTGAAACC ACATTTTTGT 
CTCAGTCCTG GCAGTCTATT TGTAGGAACA GGAGGAAACA AACTGGGCTC CAAGTATAAG 
AAAGTAGTTT ATCGAGAATA CACTGACGCC ACCTTCAGGA CAAGAATAGA AAGAGAGCCT 
CAAGAGACAC ACTTGGAGAT TTTAGGGCCT ATGATCAGAG CTGAAGTTGG CCAGGTTTTG 
TTGATCACGT TCATGAATAA AGCTAGTCAT GCTTACTCGA TACAAGCACA TGGAGTCAGA 
TCTTTTACTT CAAAACCTGA GGCCGTCATG CCAGGTAATA TGACTCAGTA CCAGTGGATC 
ATTCCGGAGA AATCCGGTCC AGGCGTGTCT GATCCAAACT GCATTGCTTT TGCCTACTGC 
TCAGCTGTGG ACTTTGTTAA GGATACAGCC AGTGGTCTGA TTGGGCCATT AATGATCTGC 
AGGAAGGGAA CACTGAACCA GGATAGACAG AGAACTGATG TGGACAGAGA ATTTGCTCTC 
CTTTTCATGG TGTTTGACGA GAACAAATCC TGGTACCTGG AAGAAAACAT TGAATCCTAC 
TACAACAGCT CAGAACCTCT TCTAAGAGAT GACGAGTTTC AGAAAAGCAA CAAGATGTAT 
GGGATAAATG GAAAAGTCTA TGCAAATCTT CGGGGACTGG AAATGGTGGT AGGGGAAAGA 
GTGGTCTGGT ACTTGTTTGG GATGGGTAAT GAAGCAGATA TTCACACCGT CCATTTTCAC 
GCTGAGACCT TCACCTACAA GACAGATCAT ACTCACCGTA CTGATGTGTA TGACCTGATT 
CCGGGGTCAT TTCAGACATT AGAGATGGTG GCAGAGGTCA ATGGAACATG GCTTCTGCAC 
TGTCATGTCT CGCACCACAT CCTTGGTGGA ATGGACACCA CTTTTATCAT CAAACCTCGA 
TCAGGTGGTG ACTCTGTGAG GAGAGATTTA AACATCATTT GTGTGCTGAT CACAACATTT 
ATGTTCAAAC TCATATATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021327952.1
CDS	46..3306
Translation	MHSIMEDNYPFKLIILQKTLLTFFFLRQASVFLQRGARRIGSVYKKAIYRQYTDSSYSQEVPKPPWLGYLGPVMRAEEQDVIIVHMKNFGSRRYSMHPHGVFYAKDSEGALYPDGTSDSLKQDDGVPPGGNYTYTWIVKAESAPQKDDPNCLTWAYHSHVSASQDISSGLIGALLTCKKGTLCETSTSDQHHAHREDVDQDLILMFSVVDENISWYLKENIQSFCSDPDGVDPASEDFQESNLMHAINGYVYGNLPGIEFCQNRTVALHLFGMGNEIDIHSVHFHGHTLLDRGHRVDVMSLFSATFATADMIPATIGTWLLNCQVNDHLQAGMQALFNVSSCSDEASSTAPLSGKIRKYFIAAEKVQWDYGPSGMNQVTKESLIDPGSFSEVFFGTNNSRIGGKYWKVIYREYTDKTFTLKRKQTSAQTHLGILGPVLRAEVGDTLQVMFLNKADRNYSIQPHGLQYDKPFEGVFYQDGIKRKGYEVQPGETFTYMWKLREGPSESDPPCISYLYFSSSNAVRDTNSGLAGPLLVCKKDALTSSGTQKEVDHEFFLLFTVFNENLSWYLDQNIETFGTNESDPENLDFQESNKMHAVNGFMYGNLPGLEMNKGSRVRWHLLGLGTEMDIHGIYFQGNTFEKQGTTHDTLSLFPHTAVTVFMQPDVSGVFELSCLVWDHYDSGMRQLYEVTGPRYEEPSVSPSETVEYFISAEELEWDYSPDRTWELQNFNTTEDERQVHVLHAAFVGIAVFEWLLKPHFCLSPGSLFVGTGGNKLGSKYKKVVYREYTDATFRTRIEREPQETHLEILGPMIRAEVGQVLLITFMNKASHAYSIQAHGVRSFTSKPEAVMPGNMTQYQWIIPEKSGPGVSDPNCIAFAYCSAVDFVKDTASGLIGPLMICRKGTLNQDRQRTDVDREFALLFMVFDENKSWYLEENIESYYNSSEPLLRDDEFQKSNKMYGINGKVYANLRGLEMVVGERVVWYLFGMGNEADIHTVHFHAETFTYKTDHTHRTDVYDLIPGSFQTLEMVAEVNGTWLLHCHVSHHILGGMDTTFIIKPRSGGDSVRRDLNIICVLITTFMFKLIY

Target ORF information:

RefSeq Version	XM_021472277.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio hephaestin-like 1b (hephl1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021472277.1

ORF Insert Sequence:

ATGCACTCAA TTATGGAAGA CAATTACCCG TTTAAGCTAA TAATTCTTCA GAAAACTCTA 
CTTACTTTCT TTTTCCTCAG GCAGGCATCA GTATTTCTGC AACGTGGGGC TCGGCGGATT 
GGCAGTGTGT ATAAAAAGGC AATTTACAGG CAGTACACAG ATTCTTCTTA CTCTCAGGAG 
GTCCCCAAAC CCCCCTGGCT GGGTTACCTG GGGCCCGTCA TGAGGGCTGA GGAGCAGGAT 
GTTATCATAG TGCACATGAA GAACTTTGGC TCCAGACGTT ACTCCATGCA TCCACATGGC 
GTCTTCTATG CCAAAGACTC AGAAGGTGCG TTGTATCCAG ACGGAACCTC AGACAGTCTG 
AAGCAAGATG ACGGTGTTCC TCCAGGGGGA AACTACACCT ACACCTGGAT TGTGAAAGCA 
GAATCTGCCC CTCAGAAGGA TGACCCAAAC TGCCTGACAT GGGCCTACCA TTCACACGTC 
AGTGCTTCAC AGGACATCTC CTCTGGCCTT ATTGGAGCTC TTCTCACCTG CAAGAAAGGA 
ACCCTGTGTG AGACGTCCAC TTCCGATCAG CACCACGCTC ATCGTGAGGA TGTGGACCAG 
GATCTCATCC TCATGTTCAG TGTGGTGGAT GAGAACATCA GCTGGTATCT AAAAGAGAAC 
ATCCAGAGCT TCTGCTCCGA CCCAGATGGT GTGGATCCTG CAAGTGAAGA CTTCCAAGAA 
TCCAATTTGA TGCACGCCAT TAATGGATAT GTGTATGGAA ACCTACCGGG GATTGAGTTT 
TGTCAAAACC GCACAGTTGC TCTGCATCTC TTTGGCATGG GCAATGAGAT CGACATTCAC 
TCTGTTCATT TCCACGGGCA CACACTTCTG GACCGCGGGC ACCGTGTGGA TGTCATGAGT 
CTGTTTTCTG CCACTTTTGC CACTGCAGAC ATGATTCCTG CCACAATAGG GACGTGGCTG 
TTAAACTGCC AGGTCAATGA TCATCTACAA GCTGGTATGC AGGCACTGTT CAATGTGTCG 
TCCTGCAGTG ATGAGGCGAG CTCCACGGCT CCTCTCAGTG GGAAGATCAG GAAGTATTTC 
ATTGCTGCTG AGAAGGTTCA GTGGGATTAC GGACCATCAG GGATGAACCA AGTGACCAAG 
GAGTCTCTAA TCGATCCTGG AAGCTTTTCA GAGGTTTTCT TTGGGACCAA TAATAGCCGA 
ATTGGTGGAA AGTACTGGAA GGTGATATAC AGAGAATATA CAGATAAGAC TTTCACCTTA 
AAGAGAAAGC AAACCAGTGC TCAGACTCAT CTAGGCATTC TGGGTCCAGT ACTGAGGGCA 
GAGGTTGGAG ATACACTTCA GGTGATGTTC CTGAATAAAG CTGACAGAAA CTACAGTATC 
CAGCCTCATG GACTCCAGTA CGACAAACCC TTCGAGGGAG TATTTTATCA AGATGGTATA 
AAGAGAAAAG GATATGAAGT TCAGCCAGGT GAAACGTTCA CCTACATGTG GAAGTTGAGG 
GAAGGACCGT CTGAAAGTGA CCCTCCCTGT ATTTCCTACC TGTACTTTTC CTCCAGTAAT 
GCAGTCAGAG ACACTAACTC TGGCCTCGCA GGCCCCCTGC TGGTGTGTAA GAAAGATGCG 
CTGACATCCA GTGGCACTCA GAAAGAGGTG GATCATGAGT TTTTCCTGCT TTTTACTGTG 
TTCAATGAAA ACCTCAGCTG GTACTTGGAT CAGAACATTG AAACCTTTGG CACCAATGAG 
TCAGACCCAG AAAATCTGGA CTTTCAGGAG AGCAATAAAA TGCATGCTGT GAATGGCTTC 
ATGTATGGGA ACCTACCTGG CCTTGAGATG AATAAGGGGT CCAGGGTCAG ATGGCATCTT 
TTAGGACTTG GCACAGAGAT GGACATTCAT GGCATTTACT TCCAAGGCAA CACCTTCGAG 
AAACAGGGCA CAACACATGA CACTCTGAGC CTGTTTCCAC ACACTGCAGT CACTGTTTTC 
ATGCAACCAG ATGTCAGTGG TGTATTTGAG CTGAGCTGCC TAGTGTGGGA TCACTATGAT 
AGTGGGATGA GACAACTGTA TGAAGTGACA GGACCAAGAT ATGAAGAGCC CTCAGTCTCA 
CCATCTGAGA CAGTGGAGTA TTTCATCAGT GCTGAAGAGC TGGAGTGGGA CTATTCGCCT 
GATCGCACAT GGGAACTGCA GAACTTCAAC ACAACGGAGG ATGAAAGGCA GGTCCATGTG 
CTACATGCAG CGTTTGTTGG TATTGCAGTG TTTGAATGGC TTCTGAAACC ACATTTTTGT 
CTCAGTCCTG GCAGTCTATT TGTAGGAACA GGAGGAAACA AACTGGGCTC CAAGTATAAG 
AAAGTAGTTT ATCGAGAATA CACTGACGCC ACCTTCAGGA CAAGAATAGA AAGAGAGCCT 
CAAGAGACAC ACTTGGAGAT TTTAGGGCCT ATGATCAGAG CTGAAGTTGG CCAGGTTTTG 
TTGATCACGT TCATGAATAA AGCTAGTCAT GCTTACTCGA TACAAGCACA TGGAGTCAGA 
TCTTTTACTT CAAAACCTGA GGCCGTCATG CCAGGTAATA TGACTCAGTA CCAGTGGATC 
ATTCCGGAGA AATCCGGTCC AGGCGTGTCT GATCCAAACT GCATTGCTTT TGCCTACTGC 
TCAGCTGTGG ACTTTGTTAA GGATACAGCC AGTGGTCTGA TTGGGCCATT AATGATCTGC 
AGGAAGGGAA CACTGAACCA GGATAGACAG AGAACTGATG TGGACAGAGA ATTTGCTCTC 
CTTTTCATGG TGTTTGACGA GAACAAATCC TGGTACCTGG AAGAAAACAT TGAATCCTAC 
TACAACAGCT CAGAACCTCT TCTAAGAGAT GACGAGTTTC AGAAAAGCAA CAAGATGTAT 
GGGATAAATG GAAAAGTCTA TGCAAATCTT CGGGGACTGG AAATGGTGGT AGGGGAAAGA 
GTGGTCTGGT ACTTGTTTGG GATGGGTAAT GAAGCAGATA TTCACACCGT CCATTTTCAC 
GCTGAGACCT TCACCTACAA GACAGATCAT ACTCACCGTA CTGATGTGTA TGACCTGATT 
CCGGGGTCAT TTCAGACATT AGAGATGGTG GCAGAGGTCA ATGGAACATG GCTTCTGCAC 
TGTCATGTCT CGCACCACAT CCTTGGTGGA ATGGACACCA CTTTTATCAT CAAACCTCGA 
TCAGGTGGTG ACTCTGTGAG GAGAGATTTA AACATCATTT GTGTGCTGAT CACAACATTT 
ATGTTCAAAC TCATATATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPHL1B ( XM_021479509.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021335184.1 Danio rerio hephaestin-like 1b (hephl1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa147815
Clone ID Related Accession (Same CDS sequence)	XM_021479509.1
Accession Version	XM_021479509.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	LOW QUALITY PROTEIN: hephaestin-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGCTTTCTT TCCTGGAGTT TTTGGGAAGC TTGAGCATCT TCTGTGCTGT ATTAGCCCAG 
GCGTCTGGAA TGGAAAGAAT CTTCTATATT GCGATTCGAG AGGAAGAGTG GGATTATGCA 
CCAAGTGGAC AGAATCTGAT CAATGGGAAG CGTATTGAAG AGGATGAGCA GGCATCAGTA 
TTTCTGCAAC GTGGGGCTCG GCGGATTGGC AGTGTGTATA AAAAGGCAAT TTACAGGCAG 
TACACAGATT CTTCTTACTC TCAGGAGGTC CCCAAACCCC CCTGGCTGGG TTACCTGGGG 
CCCATCATGA GGGCTGAGGA GCAGGATGTT ATCATAGTGC ACATGAAGAA CTTTGGCTCC 
AGACGTTACT CCATGCATCC ACATGGCGTC TTCTATGCCA AAGACTCAGA AGGTGCGTTG 
TATCCAGACG GAACCTCAGA CAGTCTGAAG CAAGATGACG GTGTTCCTCC AGGGGGAAAC 
TACACCTACA CCTGGATTGT GAAAGCAGAA TCTGCCCCTC AGAAGGATGA CCCAAACTGC 
CTGACATGGG CCTACCATTC ACACGTCAGT GCTTCACAGG ACATCTCCTC TGGCCTTATT 
GGAGCTCTTC TCACCTGCAA GAAAGGAACC CTGTGTGAGA CGTCCACTTC CGATCAGCAC 
CACGCTCATC GTGAGGATGT GGACCAGGAT CTCATCCTCA TGTTCAGTGT GGTGGATGAG 
AACATCAGCT GGTATCTAAA AGAGAACATC CAGAGCTTCT GCTCCGACCC AGATGGTGTG 
GATCCTGCAA GTGAAGACTT CCAAGAATCC AATTTGATGC ACGCCATTAA TGGATATGTG 
TATGGAAACC TACCGGGGAT TGAGTTTTGT CAAAACCGCA CAGTTGCTCT GCATCTCTTT 
GGCATGGGCA ATGAGATCGA CATTCACTCA GTTTATTTCC ACGGGCACAC ACTTCTGGAC 
CGCGGGCACC GTGTGGATGT CATGAGTCTG TTTTCTGCCA CTTTTGCCAC TGCAAACATG 
ATTCCTGCCA CAATAGGGAC GTGGCTGTTA AACTGCCAGG TCAATGATCA TCTGCAAGAT 
ACCTTTTGCT TATTGTCTTC AATAGCTGGT ATGCAGGCAC TGTTCAATGT GTCGTCCTGC 
AGTGATGAGG CGAGCTCCAC GGCTCCTCTC AGTGGGAAGA TCAGGAAGTA TTTCATTGCT 
GCTGAGAAGG TTCAGTGGGA TTACGGACCA TCAGGGATGA ACCAAGTGAC CAAGGAGTCT 
CTAATCGATC CTGGAAGCTT TTCAGAGGTT TTCTTTGGGA CCAATAATAG CCGAATTGGT 
GGAAAGTACT GGAAGGTGAT ATACAGAGAA TATACAGATA AGACTTTCAC CTTAAAGAGA 
AAGCAAACCA GTGCTCAGAC TCATCTAGGC ATTCTGGGTC CAGTACTGAG GGCAGAGGTT 
GGAGATACAC TTCAGGTGAT GTTCATGAAT AAAGCTGACA GAAACTACAG TATCCAGCCT 
CATGGACTCC AGTACGACAA ACCCTTCGAG GGAGTATTTT ATCAAGATGG TATAAAGAGA 
AAAGGATATG AAGTTCAGCC AGGTGAAACG TTCACCTACA TGTGGAAGTT GAGGGAAGGA 
CCGTCTGAAA GTGACCCCCC CTGTATTTCC TACCTGTACT TTTCCTCCAG TGATGCAGTC 
AGAGACACTA ACTCTGGCCT CGCAGGCCCC CTGCTGGTGT GTAAGAAAGA TGCGCTGACA 
TCAAGTGGCA CTCAGAAAGA GGTGGATCAT GAGTTTTTCC TGCTTTTTAC TGTGTTCAAT 
GAAAACCTCA GCTGGTACTT GGATCAGAAC ATTGAAACCT TTGGCACCAA TGAGTCAGAC 
CCAGAAAATC TGGACTTTCA GGAGAGCAAT AAAATGCATG CTGTGAATGG CTTCATGTAT 
GGGAACCTAC CTGGCCTTGA GATGAATAAG GGGTCCAGGG TCAGATGGCA TCTTTTAGGA 
CTTGGCACAG AGATGGACAT TCATGGCATT TACTTCCAAG GCAACACCTT CGAGAAACAG 
GGCACAACAC ATGACACTCT GAGCCTGTTT CCACACACTG CAGTCACTGT TTTCATGCAA 
CCAGATGTCA GTGGTGTATT TGAGCTGAGC TGTCTAGTGT GGGATCACTA TGATAGTGGG 
ATGAGACAAC TGTATGAAGT GACAGGACCA AGATATGAAG AGCCCTCAGT CTCACCATCT 
GAGACAGTGG AGTATTTCAT CAGTGCTGAA GAGCTGGAGT GGGACTATTC GCCTGATCGC 
ACATGGGAAC TGCAGAACTT CAACACAACG GAGGATGAAA GGCAGGTCTG TGCTATAGCG 
TTTGTTGGTA TTGCAGTGTT TGAATGGCTT CTGAAACCAC ATTTTTGTCT CAGTCCTGGC 
AGTCTATTTG TAGGAACAGG AGGAAACAAA CTGGGCTCCA AGTATAAGAA AGTAGTTTAT 
CGAGAATACA CCGACGCCAC CTTCAGGACA AGAATAGAAA GAGAACCTCA TGAGACACAC 
TTGGAGATTT TAGGGCCTAT GATCAGAGCT GAAGTTGGCC AGGTTTTGTT GATCACGTTC 
ATGAATAAAG CTAGTCATGC TTACTCGATA CAAGCACATG GAGTCAGATC TTTTACTTCA 
AAACCTGAGG CCGTCATGCC AGGTAATATG ACTCAGTACC AGTGGATCAT TCCGGAGAAA 
TCCGGTCCAG GCGTGTCTGA TCCAAACTGC ATTGCTTTTG CCTACTGCTC AGCTGTGGAC 
TTTGTTAAGG ATACAGCCAG TGGTCTGATT GGGCCATTAA TGATCTGCAG GAAGGGAACA 
CTGAACCAGG ATAGACAGAG AACTGATGTG GACAGAGAAT TTGCTCTCCT TTTCATGGTG 
TTTGACGAGA ACAAATCCTG GTACCTGGAA GAAAACATTG AATCCTACTA CAACAGCTCA 
GAACCTCTTC TAAGAGATGA CGAGTTTCAG AAAAGCAACA AGATGTATGG GATAAATGGA 
AAAGTCTATG CAAATCTTCG GGGACTGGAA ATGGTGGTAG GGGAAAGAGT GGTCTGGTAC 
TTGTTTGGGA TGGGTAATGA AGCAGATATT CACACCGTCC ATTTTCACGC TGAGACCTTC 
ACCTACAAGA CAGATCATAC TCACCGTACT GATGTGTATG ACCTGATTCC GGGGTCATTT 
CAGACATTAG AGATGGTGGC AGAGGTCAAT GGAACATGGC TTCTGCACTG TCATGTCTCA 
CACCACATCC TTGGTGGAAT GGACACCACT TTTATCATCA AACCTCGATC AGGTGGTGAC 
TCTGTGAGGA GAGATTTAAA CATCATTTGT GTGCTGATCA CAACATTCAT GTTCAAACTC 
ATATATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335184.1
CDS	116..3484
Translation	MLSFLEFLGSLSIFCAVLAQASGMERIFYIAIREEEWDYAPSGQNLINGKRIEEDEQASVFLQRGARRIGSVYKKAIYRQYTDSSYSQEVPKPPWLGYLGPIMRAEEQDVIIVHMKNFGSRRYSMHPHGVFYAKDSEGALYPDGTSDSLKQDDGVPPGGNYTYTWIVKAESAPQKDDPNCLTWAYHSHVSASQDISSGLIGALLTCKKGTLCETSTSDQHHAHREDVDQDLILMFSVVDENISWYLKENIQSFCSDPDGVDPASEDFQESNLMHAINGYVYGNLPGIEFCQNRTVALHLFGMGNEIDIHSVYFHGHTLLDRGHRVDVMSLFSATFATANMIPATIGTWLLNCQVNDHLQDTFCLLSSIAGMQALFNVSSCSDEASSTAPLSGKIRKYFIAAEKVQWDYGPSGMNQVTKESLIDPGSFSEVFFGTNNSRIGGKYWKVIYREYTDKTFTLKRKQTSAQTHLGILGPVLRAEVGDTLQVMFMNKADRNYSIQPHGLQYDKPFEGVFYQDGIKRKGYEVQPGETFTYMWKLREGPSESDPPCISYLYFSSSDAVRDTNSGLAGPLLVCKKDALTSSGTQKEVDHEFFLLFTVFNENLSWYLDQNIETFGTNESDPENLDFQESNKMHAVNGFMYGNLPGLEMNKGSRVRWHLLGLGTEMDIHGIYFQGNTFEKQGTTHDTLSLFPHTAVTVFMQPDVSGVFELSCLVWDHYDSGMRQLYEVTGPRYEEPSVSPSETVEYFISAEELEWDYSPDRTWELQNFNTTEDERQVCAIAFVGIAVFEWLLKPHFCLSPGSLFVGTGGNKLGSKYKKVVYREYTDATFRTRIEREPHETHLEILGPMIRAEVGQVLLITFMNKASHAYSIQAHGVRSFTSKPEAVMPGNMTQYQWIIPEKSGPGVSDPNCIAFAYCSAVDFVKDTASGLIGPLMICRKGTLNQDRQRTDVDREFALLFMVFDENKSWYLEENIESYYNSSEPLLRDDEFQKSNKMYGINGKVYANLRGLEMVVGERVVWYLFGMGNEADIHTVHFHAETFTYKTDHTHRTDVYDLIPGSFQTLEMVAEVNGTWLLHCHVSHHILGGMDTTFIIKPRSGGDSVRRDLNIICVLITTFMFKLIY

Target ORF information:

RefSeq Version	XM_021479509.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio hephaestin-like 1b (hephl1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479509.1

ORF Insert Sequence:

ATGCTTTCTT TCCTGGAGTT TTTGGGAAGC TTGAGCATCT TCTGTGCTGT ATTAGCCCAG 
GCGTCTGGAA TGGAAAGAAT CTTCTATATT GCGATTCGAG AGGAAGAGTG GGATTATGCA 
CCAAGTGGAC AGAATCTGAT CAATGGGAAG CGTATTGAAG AGGATGAGCA GGCATCAGTA 
TTTCTGCAAC GTGGGGCTCG GCGGATTGGC AGTGTGTATA AAAAGGCAAT TTACAGGCAG 
TACACAGATT CTTCTTACTC TCAGGAGGTC CCCAAACCCC CCTGGCTGGG TTACCTGGGG 
CCCATCATGA GGGCTGAGGA GCAGGATGTT ATCATAGTGC ACATGAAGAA CTTTGGCTCC 
AGACGTTACT CCATGCATCC ACATGGCGTC TTCTATGCCA AAGACTCAGA AGGTGCGTTG 
TATCCAGACG GAACCTCAGA CAGTCTGAAG CAAGATGACG GTGTTCCTCC AGGGGGAAAC 
TACACCTACA CCTGGATTGT GAAAGCAGAA TCTGCCCCTC AGAAGGATGA CCCAAACTGC 
CTGACATGGG CCTACCATTC ACACGTCAGT GCTTCACAGG ACATCTCCTC TGGCCTTATT 
GGAGCTCTTC TCACCTGCAA GAAAGGAACC CTGTGTGAGA CGTCCACTTC CGATCAGCAC 
CACGCTCATC GTGAGGATGT GGACCAGGAT CTCATCCTCA TGTTCAGTGT GGTGGATGAG 
AACATCAGCT GGTATCTAAA AGAGAACATC CAGAGCTTCT GCTCCGACCC AGATGGTGTG 
GATCCTGCAA GTGAAGACTT CCAAGAATCC AATTTGATGC ACGCCATTAA TGGATATGTG 
TATGGAAACC TACCGGGGAT TGAGTTTTGT CAAAACCGCA CAGTTGCTCT GCATCTCTTT 
GGCATGGGCA ATGAGATCGA CATTCACTCA GTTTATTTCC ACGGGCACAC ACTTCTGGAC 
CGCGGGCACC GTGTGGATGT CATGAGTCTG TTTTCTGCCA CTTTTGCCAC TGCAAACATG 
ATTCCTGCCA CAATAGGGAC GTGGCTGTTA AACTGCCAGG TCAATGATCA TCTGCAAGAT 
ACCTTTTGCT TATTGTCTTC AATAGCTGGT ATGCAGGCAC TGTTCAATGT GTCGTCCTGC 
AGTGATGAGG CGAGCTCCAC GGCTCCTCTC AGTGGGAAGA TCAGGAAGTA TTTCATTGCT 
GCTGAGAAGG TTCAGTGGGA TTACGGACCA TCAGGGATGA ACCAAGTGAC CAAGGAGTCT 
CTAATCGATC CTGGAAGCTT TTCAGAGGTT TTCTTTGGGA CCAATAATAG CCGAATTGGT 
GGAAAGTACT GGAAGGTGAT ATACAGAGAA TATACAGATA AGACTTTCAC CTTAAAGAGA 
AAGCAAACCA GTGCTCAGAC TCATCTAGGC ATTCTGGGTC CAGTACTGAG GGCAGAGGTT 
GGAGATACAC TTCAGGTGAT GTTCATGAAT AAAGCTGACA GAAACTACAG TATCCAGCCT 
CATGGACTCC AGTACGACAA ACCCTTCGAG GGAGTATTTT ATCAAGATGG TATAAAGAGA 
AAAGGATATG AAGTTCAGCC AGGTGAAACG TTCACCTACA TGTGGAAGTT GAGGGAAGGA 
CCGTCTGAAA GTGACCCCCC CTGTATTTCC TACCTGTACT TTTCCTCCAG TGATGCAGTC 
AGAGACACTA ACTCTGGCCT CGCAGGCCCC CTGCTGGTGT GTAAGAAAGA TGCGCTGACA 
TCAAGTGGCA CTCAGAAAGA GGTGGATCAT GAGTTTTTCC TGCTTTTTAC TGTGTTCAAT 
GAAAACCTCA GCTGGTACTT GGATCAGAAC ATTGAAACCT TTGGCACCAA TGAGTCAGAC 
CCAGAAAATC TGGACTTTCA GGAGAGCAAT AAAATGCATG CTGTGAATGG CTTCATGTAT 
GGGAACCTAC CTGGCCTTGA GATGAATAAG GGGTCCAGGG TCAGATGGCA TCTTTTAGGA 
CTTGGCACAG AGATGGACAT TCATGGCATT TACTTCCAAG GCAACACCTT CGAGAAACAG 
GGCACAACAC ATGACACTCT GAGCCTGTTT CCACACACTG CAGTCACTGT TTTCATGCAA 
CCAGATGTCA GTGGTGTATT TGAGCTGAGC TGTCTAGTGT GGGATCACTA TGATAGTGGG 
ATGAGACAAC TGTATGAAGT GACAGGACCA AGATATGAAG AGCCCTCAGT CTCACCATCT 
GAGACAGTGG AGTATTTCAT CAGTGCTGAA GAGCTGGAGT GGGACTATTC GCCTGATCGC 
ACATGGGAAC TGCAGAACTT CAACACAACG GAGGATGAAA GGCAGGTCTG TGCTATAGCG 
TTTGTTGGTA TTGCAGTGTT TGAATGGCTT CTGAAACCAC ATTTTTGTCT CAGTCCTGGC 
AGTCTATTTG TAGGAACAGG AGGAAACAAA CTGGGCTCCA AGTATAAGAA AGTAGTTTAT 
CGAGAATACA CCGACGCCAC CTTCAGGACA AGAATAGAAA GAGAACCTCA TGAGACACAC 
TTGGAGATTT TAGGGCCTAT GATCAGAGCT GAAGTTGGCC AGGTTTTGTT GATCACGTTC 
ATGAATAAAG CTAGTCATGC TTACTCGATA CAAGCACATG GAGTCAGATC TTTTACTTCA 
AAACCTGAGG CCGTCATGCC AGGTAATATG ACTCAGTACC AGTGGATCAT TCCGGAGAAA 
TCCGGTCCAG GCGTGTCTGA TCCAAACTGC ATTGCTTTTG CCTACTGCTC AGCTGTGGAC 
TTTGTTAAGG ATACAGCCAG TGGTCTGATT GGGCCATTAA TGATCTGCAG GAAGGGAACA 
CTGAACCAGG ATAGACAGAG AACTGATGTG GACAGAGAAT TTGCTCTCCT TTTCATGGTG 
TTTGACGAGA ACAAATCCTG GTACCTGGAA GAAAACATTG AATCCTACTA CAACAGCTCA 
GAACCTCTTC TAAGAGATGA CGAGTTTCAG AAAAGCAACA AGATGTATGG GATAAATGGA 
AAAGTCTATG CAAATCTTCG GGGACTGGAA ATGGTGGTAG GGGAAAGAGT GGTCTGGTAC 
TTGTTTGGGA TGGGTAATGA AGCAGATATT CACACCGTCC ATTTTCACGC TGAGACCTTC 
ACCTACAAGA CAGATCATAC TCACCGTACT GATGTGTATG ACCTGATTCC GGGGTCATTT 
CAGACATTAG AGATGGTGGC AGAGGTCAAT GGAACATGGC TTCTGCACTG TCATGTCTCA 
CACCACATCC TTGGTGGAAT GGACACCACT TTTATCATCA AACCTCGATC AGGTGGTGAC 
TCTGTGAGGA GAGATTTAAA CATCATTTGT GTGCTGATCA CAACATTCAT GTTCAAACTC 
ATATATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPHL1B ( XM_017358041.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017213530.1 Danio rerio hephaestin-like 1b (hephl1b), mRNA.

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CloneID	ODa54095
Clone ID Related Accession (Same CDS sequence)	XM_017358041.1
Accession Version	XM_017358041.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	LOW QUALITY PROTEIN: hephaestin-like protein 1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGCTTTCTT TCCTGGAGTT TTTGGGAAGC TTGAGCATCT TCTGTGCTGT ATTAGCCCAG 
GCGTCTGGAA TGGAAAGAAT CTTCTATATT GCGATTCGAG AGGAAGAGTG GGATTATGCA 
CCAAGTGGAC AGAATCTGAT CAATGGGAAG CGTATTGAAG AGGATGAGCA GGCATCAGTA 
TTTCTGCAAC GTGGGGCTCG GCGGATTGGC AGTGTGTATA AAAAGGCAAT TTACAGGCAG 
TACACAGATT CTTCTTACTC TCAGGAGGTC CCCAAACCCC CCTGGCTGGG TTACCTGGGG 
CCCATCATGA GGGCTGAGGA GCAGGATGTT ATCATAGTGC ACATGAAGAA CTTTGGCTCC 
AGACGTTACT CCATGCATCC ACATGGCGTC TTCTATGCCA AAGACTCAGA AGGTGCGTTG 
TATCCAGACG GAACCTCAGA CAGTCTGAAG CAAGATGACG GTGTTCCTCC AGGGGGAAAC 
TACACCTACA CCTGGATTGT GAAAGCAGAA TCTGCCCCTC AGAAGGATGA CCCAAACTGC 
CTGACATGGG CCTACCATTC ACACGTCAGT GCTTCACAGG ACATCTCCTC TGGCCTTATT 
GGAGCTCTTC TCACCTGCAA GAAAGGAACC CTGTGTGAGA CGTCCACTTC CGATCAGCAC 
CACGCTCATC GTGAGGATGT GGACCAGGAT CTCATCCTCA TGTTCAGTGT GGTGGATGAG 
AACATCAGCT GGTATCTAAA AGAGAACATC CAGAGCTTCT GCTCCGACCC AGATGGTGTG 
GATCCTGCAA GTGAAGACTT CCAAGAATCC AATTTGATGC ACGCCATTAA TGGATATGTG 
TATGGAAACC TACCGGGGAT TGAGTTTTGT CAAAACCGCA CAGTTGCTCT GCATCTCTTT 
GGCATGGGCA ATGAGATCGA CATTCACTCA GTTTATTTCC ACGGGCACAC ACTTCTGGAC 
CGCGGGCACC GTGTGGATGT CATGAGTCTG TTTTCTGCCA CTTTTGCCAC TGCAAACATG 
ATTCCTGCCA CAATAGGGAC GTGGCTGTTA AACTGCCAGG TCAATGATCA TCTGCAAGCT 
GGTATGCAGG CACTGTTCAA TGTGTCGTCC TGCAGTGATG AGGCGAGCTC CACGGCTCCT 
CTCAGTGGGA AGATCAGGAA GTATTTCATT GCTGCTGAGA AGGTTCAGTG GGATTACGGA 
CCATCAGGGA TGAACCAAGT GACCAAGGAG TCTCTAATCG ATCCTGGAAG CTTTTCAGAG 
GTTTTCTTTG GGACCAATAA TAGCCGAATT GGTGGAAAGT ACTGGAAGGT GATATACAGA 
GAATATACAG ATAAGACTTT CACCTTAAAG AGAAAGCAAA CCAGTGCTCA GACTCATCTA 
GGCATTCTGG GTCCAGTACT GAGGGCAGAG GTTGGAGATA CACTTCAGGT GATGTTCATG 
AATAAAGCTG ACAGAAACTA CAGTATCCAG CCTCATGGAC TCCAGTACGA CAAACCCTTC 
GAGGGAGTAT TTTATCAAGA TGGTATAAAG AGAAAAGGAT ATGAAGTTCA GCCAGGTGAA 
ACGTTCACCT ACATGTGGAA GTTGAGGGAA GGACCGTCTG AAAGTGACCC CCCCTGTATT 
TCCTACCTGT ACTTTTCCTC CAGTGATGCA GTCAGAGACA CTAACTCTGG CCTCGCAGGC 
CCCCTGCTGG TGTGTAAGAA AGATGCGCTG ACATCAAGTG GCACTCAGAA AGAGGTGGAT 
CATGAGTTTT TCCTGCTTTT TACTGTGTTC AATGAAAACC TCAGCTGGTA CTTGGATCAG 
AACATTGAAA CCTTTGGCAC CAATGAGTCA GACCCAGAAA ATCTGGACTT TCAGGAGAGC 
AATAAAATGC ATGCTGTGAA TGGCTTCATG TATGGGAACC TACCTGGCCT TGAGATGAAT 
AAGGGGTCCA GGGTCAGATG GCATCTTTTA GGACTTGGCA CAGAGATGGA CATTCATGGC 
ATTTACTTCC AAGGCAACAC CTTCGAGAAA CAGGGCACAA CACATGACAC TCTGAGCCTG 
TTTCCACACA CTGCAGTCAC TGTTTTCATG CAACCAGATG TCAGTGGTGT ATTTGAGCTG 
AGCTGTCTAG TGTGGGATCA CTATGATAGT GGGATGAGAC AACTGTATGA AGTGACAGGA 
CCAAGATATG AAGAGCCCTC AGTCTCACCA TCTGAGACAG TGGAGTATTT CATCAGTGCT 
GAAGAGCTGG AGTGGGACTA TTCGCCTGAT CGCACATGGG AACTGCAGAA CTTCAACACA 
ACGGAGGATG AAAGGCAGGT CCATGCTATA GCGTTTGTTG GTATTGCAGT GTTTGAATGG 
CTTCTGAAAC CACATTTTTG TCTCAGTCCT GGCAGTCTAT TTGTAGGAAC AGGAGGAAAC 
AAACTGGGCT CCAAGTATAA GAAAGTAGTT TATCGAGAAT ACACCGACGC CACCTTCAGG 
ACAAGAATAG AAAGAGAACC TCATGAGACA CACTTGGAGA TTTTAGGGCC TATGATCAGA 
GCTGAAGTTG GCCAGGTTTT GTTGATCACG TTCATGAATA AAGCTAGTCA TGCTTACTCG 
ATACAAGCAC ATGGAGTCAG ATCTTTTACT TCAAAACCTG AGGCCGTCAT GCCAGGTAAT 
ATGACTCAGT ACCAGTGGAT CATTCCGGAG AAATCCGGTC CAGGCGTGTC TGATCCAAAC 
TGCATTGCTT TTGCCTACTG CTCAGCTGTG GACTTTGTTA AGGATACAGC CAGTGGTCTG 
ATTGGGCCAT TAATGATCTG CAGGAAGGGA ACACTGAACC AGGATAGACA GAGAACTGAT 
GTGGACAGAG AATTTGCTCT CCTTTTCATG GTGTTTGACG AGAACAAATC CTGGTACCTG 
GAAGAAAACA TTGAATCCTA CTACAACAGC TCAGAACCTC TTCTAAGAGA TGACGAGTTT 
CAGAAAAGCA ACAAGATGTA TGGGATAAAT GGAAAAGTCT ATGCAAATCT TCGGGGACTG 
GAAATGGTGG TAGGGGAAAG AGTGGTCTGG TACTTGTTTG GGATGGGTAA TGAAGCAGAT 
ATTCACACCG TCCATTTTCA CGCTGAGACC TTCACCTACA AGACAGATCA TACTCACCGT 
ACTGATGTGT ATGACCTGAT TCCGGGGTCA TTTCAGACAT TAGAGATGGT GGCAGAGGTC 
AATGGAACAT GGCTTCTGCA CTGTCATGTC TCACACCACA TCCTTGGTGG AATGGACACC 
ACTTTTATCA TCAAACCTCG ATCAGGTGGT GACTCTGTGA GGAGAGATTT AAACATCATT 
TGTGTGCTGA TCACAACATT CATGTTCAAA CTCATATATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213530.1
CDS	70..3411
Translation	MLSFLEFLGSLSIFCAVLAQASGMERIFYIAIREEEWDYAPSGQNLINGKRIEEDEQASVFLQRGARRIGSVYKKAIYRQYTDSSYSQEVPKPPWLGYLGPIMRAEEQDVIIVHMKNFGSRRYSMHPHGVFYAKDSEGALYPDGTSDSLKQDDGVPPGGNYTYTWIVKAESAPQKDDPNCLTWAYHSHVSASQDISSGLIGALLTCKKGTLCETSTSDQHHAHREDVDQDLILMFSVVDENISWYLKENIQSFCSDPDGVDPASEDFQESNLMHAINGYVYGNLPGIEFCQNRTVALHLFGMGNEIDIHSVYFHGHTLLDRGHRVDVMSLFSATFATANMIPATIGTWLLNCQVNDHLQAGMQALFNVSSCSDEASSTAPLSGKIRKYFIAAEKVQWDYGPSGMNQVTKESLIDPGSFSEVFFGTNNSRIGGKYWKVIYREYTDKTFTLKRKQTSAQTHLGILGPVLRAEVGDTLQVMFMNKADRNYSIQPHGLQYDKPFEGVFYQDGIKRKGYEVQPGETFTYMWKLREGPSESDPPCISYLYFSSSDAVRDTNSGLAGPLLVCKKDALTSSGTQKEVDHEFFLLFTVFNENLSWYLDQNIETFGTNESDPENLDFQESNKMHAVNGFMYGNLPGLEMNKGSRVRWHLLGLGTEMDIHGIYFQGNTFEKQGTTHDTLSLFPHTAVTVFMQPDVSGVFELSCLVWDHYDSGMRQLYEVTGPRYEEPSVSPSETVEYFISAEELEWDYSPDRTWELQNFNTTEDERQVHAIAFVGIAVFEWLLKPHFCLSPGSLFVGTGGNKLGSKYKKVVYREYTDATFRTRIEREPHETHLEILGPMIRAEVGQVLLITFMNKASHAYSIQAHGVRSFTSKPEAVMPGNMTQYQWIIPEKSGPGVSDPNCIAFAYCSAVDFVKDTASGLIGPLMICRKGTLNQDRQRTDVDREFALLFMVFDENKSWYLEENIESYYNSSEPLLRDDEFQKSNKMYGINGKVYANLRGLEMVVGERVVWYLFGMGNEADIHTVHFHAETFTYKTDHTHRTDVYDLIPGSFQTLEMVAEVNGTWLLHCHVSHHILGGMDTTFIIKPRSGGDSVRRDLNIICVLITTFMFKLIY

Target ORF information:

RefSeq Version	XM_017358041.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio hephaestin-like 1b (hephl1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358041.1

ORF Insert Sequence:

ATGCTTTCTT TCCTGGAGTT TTTGGGAAGC TTGAGCATCT TCTGTGCTGT ATTAGCCCAG 
GCGTCTGGAA TGGAAAGAAT CTTCTATATT GCGATTCGAG AGGAAGAGTG GGATTATGCA 
CCAAGTGGAC AGAATCTGAT CAATGGGAAG CGTATTGAAG AGGATGAGCA GGCATCAGTA 
TTTCTGCAAC GTGGGGCTCG GCGGATTGGC AGTGTGTATA AAAAGGCAAT TTACAGGCAG 
TACACAGATT CTTCTTACTC TCAGGAGGTC CCCAAACCCC CCTGGCTGGG TTACCTGGGG 
CCCATCATGA GGGCTGAGGA GCAGGATGTT ATCATAGTGC ACATGAAGAA CTTTGGCTCC 
AGACGTTACT CCATGCATCC ACATGGCGTC TTCTATGCCA AAGACTCAGA AGGTGCGTTG 
TATCCAGACG GAACCTCAGA CAGTCTGAAG CAAGATGACG GTGTTCCTCC AGGGGGAAAC 
TACACCTACA CCTGGATTGT GAAAGCAGAA TCTGCCCCTC AGAAGGATGA CCCAAACTGC 
CTGACATGGG CCTACCATTC ACACGTCAGT GCTTCACAGG ACATCTCCTC TGGCCTTATT 
GGAGCTCTTC TCACCTGCAA GAAAGGAACC CTGTGTGAGA CGTCCACTTC CGATCAGCAC 
CACGCTCATC GTGAGGATGT GGACCAGGAT CTCATCCTCA TGTTCAGTGT GGTGGATGAG 
AACATCAGCT GGTATCTAAA AGAGAACATC CAGAGCTTCT GCTCCGACCC AGATGGTGTG 
GATCCTGCAA GTGAAGACTT CCAAGAATCC AATTTGATGC ACGCCATTAA TGGATATGTG 
TATGGAAACC TACCGGGGAT TGAGTTTTGT CAAAACCGCA CAGTTGCTCT GCATCTCTTT 
GGCATGGGCA ATGAGATCGA CATTCACTCA GTTTATTTCC ACGGGCACAC ACTTCTGGAC 
CGCGGGCACC GTGTGGATGT CATGAGTCTG TTTTCTGCCA CTTTTGCCAC TGCAAACATG 
ATTCCTGCCA CAATAGGGAC GTGGCTGTTA AACTGCCAGG TCAATGATCA TCTGCAAGCT 
GGTATGCAGG CACTGTTCAA TGTGTCGTCC TGCAGTGATG AGGCGAGCTC CACGGCTCCT 
CTCAGTGGGA AGATCAGGAA GTATTTCATT GCTGCTGAGA AGGTTCAGTG GGATTACGGA 
CCATCAGGGA TGAACCAAGT GACCAAGGAG TCTCTAATCG ATCCTGGAAG CTTTTCAGAG 
GTTTTCTTTG GGACCAATAA TAGCCGAATT GGTGGAAAGT ACTGGAAGGT GATATACAGA 
GAATATACAG ATAAGACTTT CACCTTAAAG AGAAAGCAAA CCAGTGCTCA GACTCATCTA 
GGCATTCTGG GTCCAGTACT GAGGGCAGAG GTTGGAGATA CACTTCAGGT GATGTTCATG 
AATAAAGCTG ACAGAAACTA CAGTATCCAG CCTCATGGAC TCCAGTACGA CAAACCCTTC 
GAGGGAGTAT TTTATCAAGA TGGTATAAAG AGAAAAGGAT ATGAAGTTCA GCCAGGTGAA 
ACGTTCACCT ACATGTGGAA GTTGAGGGAA GGACCGTCTG AAAGTGACCC CCCCTGTATT 
TCCTACCTGT ACTTTTCCTC CAGTGATGCA GTCAGAGACA CTAACTCTGG CCTCGCAGGC 
CCCCTGCTGG TGTGTAAGAA AGATGCGCTG ACATCAAGTG GCACTCAGAA AGAGGTGGAT 
CATGAGTTTT TCCTGCTTTT TACTGTGTTC AATGAAAACC TCAGCTGGTA CTTGGATCAG 
AACATTGAAA CCTTTGGCAC CAATGAGTCA GACCCAGAAA ATCTGGACTT TCAGGAGAGC 
AATAAAATGC ATGCTGTGAA TGGCTTCATG TATGGGAACC TACCTGGCCT TGAGATGAAT 
AAGGGGTCCA GGGTCAGATG GCATCTTTTA GGACTTGGCA CAGAGATGGA CATTCATGGC 
ATTTACTTCC AAGGCAACAC CTTCGAGAAA CAGGGCACAA CACATGACAC TCTGAGCCTG 
TTTCCACACA CTGCAGTCAC TGTTTTCATG CAACCAGATG TCAGTGGTGT ATTTGAGCTG 
AGCTGTCTAG TGTGGGATCA CTATGATAGT GGGATGAGAC AACTGTATGA AGTGACAGGA 
CCAAGATATG AAGAGCCCTC AGTCTCACCA TCTGAGACAG TGGAGTATTT CATCAGTGCT 
GAAGAGCTGG AGTGGGACTA TTCGCCTGAT CGCACATGGG AACTGCAGAA CTTCAACACA 
ACGGAGGATG AAAGGCAGGT CCATGCTATA GCGTTTGTTG GTATTGCAGT GTTTGAATGG 
CTTCTGAAAC CACATTTTTG TCTCAGTCCT GGCAGTCTAT TTGTAGGAAC AGGAGGAAAC 
AAACTGGGCT CCAAGTATAA GAAAGTAGTT TATCGAGAAT ACACCGACGC CACCTTCAGG 
ACAAGAATAG AAAGAGAACC TCATGAGACA CACTTGGAGA TTTTAGGGCC TATGATCAGA 
GCTGAAGTTG GCCAGGTTTT GTTGATCACG TTCATGAATA AAGCTAGTCA TGCTTACTCG 
ATACAAGCAC ATGGAGTCAG ATCTTTTACT TCAAAACCTG AGGCCGTCAT GCCAGGTAAT 
ATGACTCAGT ACCAGTGGAT CATTCCGGAG AAATCCGGTC CAGGCGTGTC TGATCCAAAC 
TGCATTGCTT TTGCCTACTG CTCAGCTGTG GACTTTGTTA AGGATACAGC CAGTGGTCTG 
ATTGGGCCAT TAATGATCTG CAGGAAGGGA ACACTGAACC AGGATAGACA GAGAACTGAT 
GTGGACAGAG AATTTGCTCT CCTTTTCATG GTGTTTGACG AGAACAAATC CTGGTACCTG 
GAAGAAAACA TTGAATCCTA CTACAACAGC TCAGAACCTC TTCTAAGAGA TGACGAGTTT 
CAGAAAAGCA ACAAGATGTA TGGGATAAAT GGAAAAGTCT ATGCAAATCT TCGGGGACTG 
GAAATGGTGG TAGGGGAAAG AGTGGTCTGG TACTTGTTTG GGATGGGTAA TGAAGCAGAT 
ATTCACACCG TCCATTTTCA CGCTGAGACC TTCACCTACA AGACAGATCA TACTCACCGT 
ACTGATGTGT ATGACCTGAT TCCGGGGTCA TTTCAGACAT TAGAGATGGT GGCAGAGGTC 
AATGGAACAT GGCTTCTGCA CTGTCATGTC TCACACCACA TCCTTGGTGG AATGGACACC 
ACTTTTATCA TCAAACCTCG ATCAGGTGGT GACTCTGTGA GGAGAGATTT AAACATCATT 
TGTGTGCTGA TCACAACATT CATGTTCAAA CTCATATATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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HEPHL1B cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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