HEPHL1 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	HEPHL1
Entrez Gene ID	485127
Full Name	hephaestin like 1
General protein information	Preferred Names hephaestin like 1 Names hephaestin-like protein 1
Gene Type	protein-coding
Organism	Canis lupus familiaris(Dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_542245.6	XP_542245.3	hephaestin-like protein 1
XM_542245.5	XP_542245.3	hephaestin-like protein 1

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	hephl1	XP_002939767.2
Homo sapiens (human)	HEPHL1	NP_001092142.1
Macaca mulatta (Rhesus monkey)	HEPHL1	XP_001086917.1
Gallus gallus (chicken)	HEPHL1	XP_417192.4
Danio rerio (zebrafish)	LOC101886332	XP_005155347.1
Pan troglodytes (chimpanzee)	HEPHL1	XP_508699.2
Canis lupus familiaris (dog)	HEPHL1	XP_542245.3
Bos taurus (cattle)	HEPHL1	NP_001179440.1
Mus musculus (house mouse)	Hephl1	NP_001158269.1
Rattus norvegicus (Norway rat)	Hephl1	NP_001178922.1

Gene Ontology

The following HEPHL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HEPHL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe22785	XM_542245.6 Latest version!	Canis lupus familiaris hephaestin like 1 (HEPHL1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OCe22785	XM_542245.5	Canis lupus familiaris hephaestin-like 1 (HEPHL1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HEPHL1 ( XM_542245.6 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_542245.3 Canis lupus familiaris hephaestin like 1 (HEPHL1), mRNA.

Price & Availability↑

CloneID	OCe22785
Clone ID Related Accession (Same CDS sequence)	XM_542245.6 , XM_542245.5
Accession Version	XM_542245.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	hephaestin-like protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006603.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_542245.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCGGA AGCAGCAGGC TGACTGCATC GTTCTCTTCA CATTCCTGGG TCTGTCTGGG 
CTGGTTGGTG CAGTTACCAG AACATACCAC ATTGGGATTG TGGAAGAATA CTGGAACTAT 
GTACCCCAAG GGAAGAATGT TGTTACTGGG GGAAGTTTTT CAGAAGACAA GCTTGCAACC 
TTATTTCTCG AAAGAGGGCC CAACAGGATA GGTGGTCTTT ACAAAAAGGC TGTTTACAGA 
CATTATACAG ATGAGACGTA TTCCACAGAG ATTCCCAAAC CTCCCTGGCT CGGATTCCTG 
GGCCCTATCT TGAGGGCTGA AGTGGGTGAT GTGATTGTCA TTCATTTGAA GAACTTTGCT 
TCCCGACCCT ACTCTTTGCA TCCACATGGT GTTTTCTACA ACAAAGATTC AGAAGGAGCT 
CTATACCCAG ATGGAACATC CGGAAGGAAT AAGAATGATG ATATGGTTCC TCCTGGCAAA 
AACTACACCT ATGTCTGGCC AGTAAGAGAA GAATATGCCC CTGCTCCAGC AGATGCCAAC 
TGTCTGACCT GGGTGTACCA TTCACACATT GATGCTCCTA AGGATATCTG CTCTGGGTTA 
ATTGGCCCGC TCCTGGTGTG CAAGGAAGGT ATCCTGAATA AATATACAGG CACAAGGACA 
GATGTGGACC GAGAGTTCAT TATCATGTTT ACTCTGGTGG ATGAGAATCA AAGTTGGTAC 
CTTGATGAAA ATATCAGACA TTTCTGCACC AACCCTGACT CAGTTGACAA AAAAGATGCT 
GTTTTCCAGA GAAGTAACAA AATGCATGCC CTCAATGGAT ATCTCTTTGG AAACCTCCCT 
GAGCCTGATA TGTGTGTTGG TGAATCAGTG TCCTGGCACT TATTTGGGAT GGGGAATGAG 
ATAGACATCC ATTCCATCTA TTTCTATGGA AACACCTTCA TCAGCAGAGG GCATCGGACT 
GATGTCGTCA ACCTGTTCCC AGCCACCTTC CTGACAACAG AAATGGTGGT TGAGAATCCT 
GGGAAGTGGA TGATAACTTG CCAAGTCAGT GATCACCTAC AAGCTGGCAT GTTGGGACAG 
TACAATGTTG GTAACTGCAA AGGTGATATT TCTCATCCCA AGATGAAGGG TCAGCAGAGA 
CACTACTTTA TAGCAGCCGA AAAAGTTCTT TGGGATTATG CTCCTCAAGG CTATAACAAA 
TTCAGTGGTC TTCCCCTTAA TGCCTCTGGC AGTGACTCCG AGTTCTACTT CACACAAGGG 
GACAACAGAA TTGGAGGAAA ATATTGGAAG GCTCAGTATA TGGAGTATGT TGATGCAACT 
TTTACTCGAA GAAAGAGACT CTCTGAGGCA GAAGCCCATC TTGGAATTCT TGGCCCCGTC 
ATCAGGGCAG AGGTGGGTGA TACTCTGTTA GTGACCTTTG CCAACAAAGC CGACAAAGTC 
TACAGTATTT TGCCCCATGG CGTGATCTAT GACAAGGCGT CTGATGCAGC CCCAAACATA 
GATGGATTTC TGAAGCCAGG GGCGCATGTT AAGCCAGGTG AAACTTTCAC ATACAAGTGG 
ACGGTGCCTG AGAGTGTAAG CCCGACAGCT GGTGATCCCC CCTGTCTGAC CTATCTTTAC 
TTCTCAGCGG TTGAACCAAT CCAGGACACC AGCTCTGGCC TTGTGGGGCC TTTACTAGTC 
TGTAAAAAGG GCGTACTCAA TGCTGATGGG ACACAGAAAG GAATAGATAA GGAATTTTAC 
CTACTGTTCA CAGTCTTTGA TGAGAATCTG AGCAGGTACC TTGACGAAAA CATTCAGAGG 
TTTACATGGC GCCCCTTCAG TGTTGACAAA GAGGACAAGG ATTTTGTGAA GTCCAACCGA 
ATGCATGCGA TCAATGGCTA TATGTATGGC AACCAGCCAG GCCTAAGCAT GTGCAAAAAA 
GATAGAGTTT CCTGGCATAT GATTGGAATG GGCACCGACA CTGATATGCA TGGAGGTTAT 
TTTCAAGGGA ACACCATTCA CCTACAAGGG ACTCATCGCG ACACCCTAGC CCTGTTTCCG 
CATTTCTCTA CAACGGTGTT CATGCAGCCG GACCACGCAG GTATTTTCAG GGTGTTTTGT 
TACACCATGC ACCACTTCGT GAGAGGCATG AATCAAATAT ATGAGGTCAA CAGCTGTGGC 
AACAAGGACC CTTCTGAGCA GCCGTACGGG ATGATAAGAA CCTTTTACAT CGCTGCTGAA 
GAGGTAGAAT GGGATTATGC CCCTAACAAA AACTGGGAGT TCGAAAAGCA GCACTTGGAC 
GCAGGAGGGG AAAGACATGG AGATATATTT ATGAACCACA CTGAAAACTG GATTGGCTCT 
CAGTACAAGA AGGTAGTTTA CAGAGAATAC ACTGATGGAG AATTCGTGGA GATCAAGGCT 
CGACCACCAC GAGAGAAGCA CTTGGAACTC CTGGGCCCAA TGGTTCATGC TGAAGTGGGT 
GACTCCATCC TGATCATATT TAAGAACAAA GCCAAGCGGC CCTACTCCAT CTCAGCCCAT 
GGTATTGAAG ACATGGATAG TGGGAAGCAA CTCCATGTGC CCATCACAAA GCCAGGAGAA 
ATAAATACTT ACAGGTGGAA TGTCCCTAAA AGGTCTGGTC CAGGACCAAA TGACCCAAAT 
TGTATTCCAT GGGTTTACTA TTCAACAGTA AACTTTGTGA AGGATACATA CAGTGGTTTG 
ATGGGTCCTC TGATTATTTG CAGAGAAGGA GTGTTGAATG AGAAGGGAAG AAGAAGCGAT 
GTTGATTATG AATTCGTTCT CTTGTTTTTG GTATTTAATG AGAATGAATC CTGGTATCTG 
GATGACAATA TTAAAAAGTA TCTCAATAAA GATCCACGAT ATTTTAAGCG CAGTGACGAT 
TTTGAAGAAA GCAACAAAAT GCATGCTATC AATGGAAAGA TTTTTGGGAA TCTCCATGGC 
CTTATCATGA ATGAAGATAC GATGACAAAC TGGTATCTGT TGGGAATAGG AAGTGAAGTG 
GACATACACA CCATCCATTA TCATGCTGAG AGTTTTCTTT TCAAGATAGA TAAATCTTAC 
CGAGAAGATG TGTACGATCT CTTTCCTGGG ACATTCCAAA CCATTGAGCT GTTTGCAGAT 
CACCCAGGAA CATGGCTGAT ACACTGTCAT GTGTCTGACC ACATCCATGC TGGCATGGAG 
ACAACCTACA CAGTCCTTCG GAACATAGAC AACAGGATCC CTTACTCTAC CAAGTCTCCA 
TCTGGAGGAG CGTCCCCCCC AGCCACAGTG CCCTCCAATG AAGAACCCGG CAAGGAGCAG 
CTCTATTTCT TTGGCAAGAA TCTGCATCCT AGGGGAGCAA AAGCAGCCTT GGTCATCCTC 
TTCGTCATTG GACTCCTCTT CCTTATCTCC ACGGTGATTC TTTCCCTCAG ATTGCACTCT 
GCAAAGAAGC AGGTTGCATA TCGGCAAGTT CAGTCCTGCG CACTCCCCAC AGATGCTCTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_542245.3
CDS	32..3514
Translation	MPRKQQADCIVLFTFLGLSGLVGAVTRTYHIGIVEEYWNYVPQGKNVVTGGSFSEDKLATLFLERGPNRIGGLYKKAVYRHYTDETYSTEIPKPPWLGFLGPILRAEVGDVIVIHLKNFASRPYSLHPHGVFYNKDSEGALYPDGTSGRNKNDDMVPPGKNYTYVWPVREEYAPAPADANCLTWVYHSHIDAPKDICSGLIGPLLVCKEGILNKYTGTRTDVDREFIIMFTLVDENQSWYLDENIRHFCTNPDSVDKKDAVFQRSNKMHALNGYLFGNLPEPDMCVGESVSWHLFGMGNEIDIHSIYFYGNTFISRGHRTDVVNLFPATFLTTEMVVENPGKWMITCQVSDHLQAGMLGQYNVGNCKGDISHPKMKGQQRHYFIAAEKVLWDYAPQGYNKFSGLPLNASGSDSEFYFTQGDNRIGGKYWKAQYMEYVDATFTRRKRLSEAEAHLGILGPVIRAEVGDTLLVTFANKADKVYSILPHGVIYDKASDAAPNIDGFLKPGAHVKPGETFTYKWTVPESVSPTAGDPPCLTYLYFSAVEPIQDTSSGLVGPLLVCKKGVLNADGTQKGIDKEFYLLFTVFDENLSRYLDENIQRFTWRPFSVDKEDKDFVKSNRMHAINGYMYGNQPGLSMCKKDRVSWHMIGMGTDTDMHGGYFQGNTIHLQGTHRDTLALFPHFSTTVFMQPDHAGIFRVFCYTMHHFVRGMNQIYEVNSCGNKDPSEQPYGMIRTFYIAAEEVEWDYAPNKNWEFEKQHLDAGGERHGDIFMNHTENWIGSQYKKVVYREYTDGEFVEIKARPPREKHLELLGPMVHAEVGDSILIIFKNKAKRPYSISAHGIEDMDSGKQLHVPITKPGEINTYRWNVPKRSGPGPNDPNCIPWVYYSTVNFVKDTYSGLMGPLIICREGVLNEKGRRSDVDYEFVLLFLVFNENESWYLDDNIKKYLNKDPRYFKRSDDFEESNKMHAINGKIFGNLHGLIMNEDTMTNWYLLGIGSEVDIHTIHYHAESFLFKIDKSYREDVYDLFPGTFQTIELFADHPGTWLIHCHVSDHIHAGMETTYTVLRNIDNRIPYSTKSPSGGASPPATVPSNEEPGKEQLYFFGKNLHPRGAKAALVILFVIGLLFLISTVILSLRLHSAKKQVAYRQVQSCALPTDAL

Target ORF information:

RefSeq Version	XM_542245.6
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris hephaestin like 1 (HEPHL1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_542245.6

ORF Insert Sequence:

ATGCCTCGGA AGCAGCAGGC TGACTGCATC GTTCTCTTCA CATTCCTGGG TCTGTCTGGG 
CTGGTTGGTG CAGTTACCAG AACATACCAC ATTGGGATTG TGGAAGAATA CTGGAACTAT 
GTACCCCAAG GGAAGAATGT TGTTACTGGG GGAAGTTTTT CAGAAGACAA GCTTGCAACC 
TTATTTCTCG AAAGAGGGCC CAACAGGATA GGTGGTCTTT ACAAAAAGGC TGTTTACAGA 
CATTATACAG ATGAGACGTA TTCCACAGAG ATTCCCAAAC CTCCCTGGCT CGGATTCCTG 
GGCCCTATCT TGAGGGCTGA AGTGGGTGAT GTGATTGTCA TTCATTTGAA GAACTTTGCT 
TCCCGACCCT ACTCTTTGCA TCCACATGGT GTTTTCTACA ACAAAGATTC AGAAGGAGCT 
CTATACCCAG ATGGAACATC CGGAAGGAAT AAGAATGATG ATATGGTTCC TCCTGGCAAA 
AACTACACCT ATGTCTGGCC AGTAAGAGAA GAATATGCCC CTGCTCCAGC AGATGCCAAC 
TGTCTGACCT GGGTGTACCA TTCACACATT GATGCTCCTA AGGATATCTG CTCTGGGTTA 
ATTGGCCCGC TCCTGGTGTG CAAGGAAGGT ATCCTGAATA AATATACAGG CACAAGGACA 
GATGTGGACC GAGAGTTCAT TATCATGTTT ACTCTGGTGG ATGAGAATCA AAGTTGGTAC 
CTTGATGAAA ATATCAGACA TTTCTGCACC AACCCTGACT CAGTTGACAA AAAAGATGCT 
GTTTTCCAGA GAAGTAACAA AATGCATGCC CTCAATGGAT ATCTCTTTGG AAACCTCCCT 
GAGCCTGATA TGTGTGTTGG TGAATCAGTG TCCTGGCACT TATTTGGGAT GGGGAATGAG 
ATAGACATCC ATTCCATCTA TTTCTATGGA AACACCTTCA TCAGCAGAGG GCATCGGACT 
GATGTCGTCA ACCTGTTCCC AGCCACCTTC CTGACAACAG AAATGGTGGT TGAGAATCCT 
GGGAAGTGGA TGATAACTTG CCAAGTCAGT GATCACCTAC AAGCTGGCAT GTTGGGACAG 
TACAATGTTG GTAACTGCAA AGGTGATATT TCTCATCCCA AGATGAAGGG TCAGCAGAGA 
CACTACTTTA TAGCAGCCGA AAAAGTTCTT TGGGATTATG CTCCTCAAGG CTATAACAAA 
TTCAGTGGTC TTCCCCTTAA TGCCTCTGGC AGTGACTCCG AGTTCTACTT CACACAAGGG 
GACAACAGAA TTGGAGGAAA ATATTGGAAG GCTCAGTATA TGGAGTATGT TGATGCAACT 
TTTACTCGAA GAAAGAGACT CTCTGAGGCA GAAGCCCATC TTGGAATTCT TGGCCCCGTC 
ATCAGGGCAG AGGTGGGTGA TACTCTGTTA GTGACCTTTG CCAACAAAGC CGACAAAGTC 
TACAGTATTT TGCCCCATGG CGTGATCTAT GACAAGGCGT CTGATGCAGC CCCAAACATA 
GATGGATTTC TGAAGCCAGG GGCGCATGTT AAGCCAGGTG AAACTTTCAC ATACAAGTGG 
ACGGTGCCTG AGAGTGTAAG CCCGACAGCT GGTGATCCCC CCTGTCTGAC CTATCTTTAC 
TTCTCAGCGG TTGAACCAAT CCAGGACACC AGCTCTGGCC TTGTGGGGCC TTTACTAGTC 
TGTAAAAAGG GCGTACTCAA TGCTGATGGG ACACAGAAAG GAATAGATAA GGAATTTTAC 
CTACTGTTCA CAGTCTTTGA TGAGAATCTG AGCAGGTACC TTGACGAAAA CATTCAGAGG 
TTTACATGGC GCCCCTTCAG TGTTGACAAA GAGGACAAGG ATTTTGTGAA GTCCAACCGA 
ATGCATGCGA TCAATGGCTA TATGTATGGC AACCAGCCAG GCCTAAGCAT GTGCAAAAAA 
GATAGAGTTT CCTGGCATAT GATTGGAATG GGCACCGACA CTGATATGCA TGGAGGTTAT 
TTTCAAGGGA ACACCATTCA CCTACAAGGG ACTCATCGCG ACACCCTAGC CCTGTTTCCG 
CATTTCTCTA CAACGGTGTT CATGCAGCCG GACCACGCAG GTATTTTCAG GGTGTTTTGT 
TACACCATGC ACCACTTCGT GAGAGGCATG AATCAAATAT ATGAGGTCAA CAGCTGTGGC 
AACAAGGACC CTTCTGAGCA GCCGTACGGG ATGATAAGAA CCTTTTACAT CGCTGCTGAA 
GAGGTAGAAT GGGATTATGC CCCTAACAAA AACTGGGAGT TCGAAAAGCA GCACTTGGAC 
GCAGGAGGGG AAAGACATGG AGATATATTT ATGAACCACA CTGAAAACTG GATTGGCTCT 
CAGTACAAGA AGGTAGTTTA CAGAGAATAC ACTGATGGAG AATTCGTGGA GATCAAGGCT 
CGACCACCAC GAGAGAAGCA CTTGGAACTC CTGGGCCCAA TGGTTCATGC TGAAGTGGGT 
GACTCCATCC TGATCATATT TAAGAACAAA GCCAAGCGGC CCTACTCCAT CTCAGCCCAT 
GGTATTGAAG ACATGGATAG TGGGAAGCAA CTCCATGTGC CCATCACAAA GCCAGGAGAA 
ATAAATACTT ACAGGTGGAA TGTCCCTAAA AGGTCTGGTC CAGGACCAAA TGACCCAAAT 
TGTATTCCAT GGGTTTACTA TTCAACAGTA AACTTTGTGA AGGATACATA CAGTGGTTTG 
ATGGGTCCTC TGATTATTTG CAGAGAAGGA GTGTTGAATG AGAAGGGAAG AAGAAGCGAT 
GTTGATTATG AATTCGTTCT CTTGTTTTTG GTATTTAATG AGAATGAATC CTGGTATCTG 
GATGACAATA TTAAAAAGTA TCTCAATAAA GATCCACGAT ATTTTAAGCG CAGTGACGAT 
TTTGAAGAAA GCAACAAAAT GCATGCTATC AATGGAAAGA TTTTTGGGAA TCTCCATGGC 
CTTATCATGA ATGAAGATAC GATGACAAAC TGGTATCTGT TGGGAATAGG AAGTGAAGTG 
GACATACACA CCATCCATTA TCATGCTGAG AGTTTTCTTT TCAAGATAGA TAAATCTTAC 
CGAGAAGATG TGTACGATCT CTTTCCTGGG ACATTCCAAA CCATTGAGCT GTTTGCAGAT 
CACCCAGGAA CATGGCTGAT ACACTGTCAT GTGTCTGACC ACATCCATGC TGGCATGGAG 
ACAACCTACA CAGTCCTTCG GAACATAGAC AACAGGATCC CTTACTCTAC CAAGTCTCCA 
TCTGGAGGAG CGTCCCCCCC AGCCACAGTG CCCTCCAATG AAGAACCCGG CAAGGAGCAG 
CTCTATTTCT TTGGCAAGAA TCTGCATCCT AGGGGAGCAA AAGCAGCCTT GGTCATCCTC 
TTCGTCATTG GACTCCTCTT CCTTATCTCC ACGGTGATTC TTTCCCTCAG ATTGCACTCT 
GCAAAGAAGC AGGTTGCATA TCGGCAAGTT CAGTCCTGCG CACTCCCCAC AGATGCTCTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEPHL1 ( XM_542245.5 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_542245.3 Canis lupus familiaris hephaestin-like 1 (HEPHL1), mRNA.

Price & Availability↑

CloneID	OCe22785
Clone ID Related Accession (Same CDS sequence)	XM_542245.6 , XM_542245.5
Accession Version	XM_542245.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	hephaestin-like protein 1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726097.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_542245.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCGGA AGCAGCAGGC TGACTGCATC GTTCTCTTCA CATTCCTGGG TCTGTCTGGG 
CTGGTTGGTG CAGTTACCAG AACATACCAC ATTGGGATTG TGGAAGAATA CTGGAACTAT 
GTACCCCAAG GGAAGAATGT TGTTACTGGG GGAAGTTTTT CAGAAGACAA GCTTGCAACC 
TTATTTCTCG AAAGAGGGCC CAACAGGATA GGTGGTCTTT ACAAAAAGGC TGTTTACAGA 
CATTATACAG ATGAGACGTA TTCCACAGAG ATTCCCAAAC CTCCCTGGCT CGGATTCCTG 
GGCCCTATCT TGAGGGCTGA AGTGGGTGAT GTGATTGTCA TTCATTTGAA GAACTTTGCT 
TCCCGACCCT ACTCTTTGCA TCCACATGGT GTTTTCTACA ACAAAGATTC AGAAGGAGCT 
CTATACCCAG ATGGAACATC CGGAAGGAAT AAGAATGATG ATATGGTTCC TCCTGGCAAA 
AACTACACCT ATGTCTGGCC AGTAAGAGAA GAATATGCCC CTGCTCCAGC AGATGCCAAC 
TGTCTGACCT GGGTGTACCA TTCACACATT GATGCTCCTA AGGATATCTG CTCTGGGTTA 
ATTGGCCCGC TCCTGGTGTG CAAGGAAGGT ATCCTGAATA AATATACAGG CACAAGGACA 
GATGTGGACC GAGAGTTCAT TATCATGTTT ACTCTGGTGG ATGAGAATCA AAGTTGGTAC 
CTTGATGAAA ATATCAGACA TTTCTGCACC AACCCTGACT CAGTTGACAA AAAAGATGCT 
GTTTTCCAGA GAAGTAACAA AATGCATGCC CTCAATGGAT ATCTCTTTGG AAACCTCCCT 
GAGCCTGATA TGTGTGTTGG TGAATCAGTG TCCTGGCACT TATTTGGGAT GGGGAATGAG 
ATAGACATCC ATTCCATCTA TTTCTATGGA AACACCTTCA TCAGCAGAGG GCATCGGACT 
GATGTCGTCA ACCTGTTCCC AGCCACCTTC CTGACAACAG AAATGGTGGT TGAGAATCCT 
GGGAAGTGGA TGATAACTTG CCAAGTCAGT GATCACCTAC AAGCTGGCAT GTTGGGACAG 
TACAATGTTG GTAACTGCAA AGGTGATATT TCTCATCCCA AGATGAAGGG TCAGCAGAGA 
CACTACTTTA TAGCAGCCGA AAAAGTTCTT TGGGATTATG CTCCTCAAGG CTATAACAAA 
TTCAGTGGTC TTCCCCTTAA TGCCTCTGGC AGTGACTCCG AGTTCTACTT CACACAAGGG 
GACAACAGAA TTGGAGGAAA ATATTGGAAG GCTCAGTATA TGGAGTATGT TGATGCAACT 
TTTACTCGAA GAAAGAGACT CTCTGAGGCA GAAGCCCATC TTGGAATTCT TGGCCCCGTC 
ATCAGGGCAG AGGTGGGTGA TACTCTGTTA GTGACCTTTG CCAACAAAGC CGACAAAGTC 
TACAGTATTT TGCCCCATGG CGTGATCTAT GACAAGGCGT CTGATGCAGC CCCAAACATA 
GATGGATTTC TGAAGCCAGG GGCGCATGTT AAGCCAGGTG AAACTTTCAC ATACAAGTGG 
ACGGTGCCTG AGAGTGTAAG CCCGACAGCT GGTGATCCCC CCTGTCTGAC CTATCTTTAC 
TTCTCAGCGG TTGAACCAAT CCAGGACACC AGCTCTGGCC TTGTGGGGCC TTTACTAGTC 
TGTAAAAAGG GCGTACTCAA TGCTGATGGG ACACAGAAAG GAATAGATAA GGAATTTTAC 
CTACTGTTCA CAGTCTTTGA TGAGAATCTG AGCAGGTACC TTGACGAAAA CATTCAGAGG 
TTTACATGGC GCCCCTTCAG TGTTGACAAA GAGGACAAGG ATTTTGTGAA GTCCAACCGA 
ATGCATGCGA TCAATGGCTA TATGTATGGC AACCAGCCAG GCCTAAGCAT GTGCAAAAAA 
GATAGAGTTT CCTGGCATAT GATTGGAATG GGCACCGACA CTGATATGCA TGGAGGTTAT 
TTTCAAGGGA ACACCATTCA CCTACAAGGG ACTCATCGCG ACACCCTAGC CCTGTTTCCG 
CATTTCTCTA CAACGGTGTT CATGCAGCCG GACCACGCAG GTATTTTCAG GGTGTTTTGT 
TACACCATGC ACCACTTCGT GAGAGGCATG AATCAAATAT ATGAGGTCAA CAGCTGTGGC 
AACAAGGACC CTTCTGAGCA GCCGTACGGG ATGATAAGAA CCTTTTACAT CGCTGCTGAA 
GAGGTAGAAT GGGATTATGC CCCTAACAAA AACTGGGAGT TCGAAAAGCA GCACTTGGAC 
GCAGGAGGGG AAAGACATGG AGATATATTT ATGAACCACA CTGAAAACTG GATTGGCTCT 
CAGTACAAGA AGGTAGTTTA CAGAGAATAC ACTGATGGAG AATTCGTGGA GATCAAGGCT 
CGACCACCAC GAGAGAAGCA CTTGGAACTC CTGGGCCCAA TGGTTCATGC TGAAGTGGGT 
GACTCCATCC TGATCATATT TAAGAACAAA GCCAAGCGGC CCTACTCCAT CTCAGCCCAT 
GGTATTGAAG ACATGGATAG TGGGAAGCAA CTCCATGTGC CCATCACAAA GCCAGGAGAA 
ATAAATACTT ACAGGTGGAA TGTCCCTAAA AGGTCTGGTC CAGGACCAAA TGACCCAAAT 
TGTATTCCAT GGGTTTACTA TTCAACAGTA AACTTTGTGA AGGATACATA CAGTGGTTTG 
ATGGGTCCTC TGATTATTTG CAGAGAAGGA GTGTTGAATG AGAAGGGAAG AAGAAGCGAT 
GTTGATTATG AATTCGTTCT CTTGTTTTTG GTATTTAATG AGAATGAATC CTGGTATCTG 
GATGACAATA TTAAAAAGTA TCTCAATAAA GATCCACGAT ATTTTAAGCG CAGTGACGAT 
TTTGAAGAAA GCAACAAAAT GCATGCTATC AATGGAAAGA TTTTTGGGAA TCTCCATGGC 
CTTATCATGA ATGAAGATAC GATGACAAAC TGGTATCTGT TGGGAATAGG AAGTGAAGTG 
GACATACACA CCATCCATTA TCATGCTGAG AGTTTTCTTT TCAAGATAGA TAAATCTTAC 
CGAGAAGATG TGTACGATCT CTTTCCTGGG ACATTCCAAA CCATTGAGCT GTTTGCAGAT 
CACCCAGGAA CATGGCTGAT ACACTGTCAT GTGTCTGACC ACATCCATGC TGGCATGGAG 
ACAACCTACA CAGTCCTTCG GAACATAGAC AACAGGATCC CTTACTCTAC CAAGTCTCCA 
TCTGGAGGAG CGTCCCCCCC AGCCACAGTG CCCTCCAATG AAGAACCCGG CAAGGAGCAG 
CTCTATTTCT TTGGCAAGAA TCTGCATCCT AGGGGAGCAA AAGCAGCCTT GGTCATCCTC 
TTCGTCATTG GACTCCTCTT CCTTATCTCC ACGGTGATTC TTTCCCTCAG ATTGCACTCT 
GCAAAGAAGC AGGTTGCATA TCGGCAAGTT CAGTCCTGCG CACTCCCCAC AGATGCTCTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_542245.3
CDS	13..3495
Translation	MPRKQQADCIVLFTFLGLSGLVGAVTRTYHIGIVEEYWNYVPQGKNVVTGGSFSEDKLATLFLERGPNRIGGLYKKAVYRHYTDETYSTEIPKPPWLGFLGPILRAEVGDVIVIHLKNFASRPYSLHPHGVFYNKDSEGALYPDGTSGRNKNDDMVPPGKNYTYVWPVREEYAPAPADANCLTWVYHSHIDAPKDICSGLIGPLLVCKEGILNKYTGTRTDVDREFIIMFTLVDENQSWYLDENIRHFCTNPDSVDKKDAVFQRSNKMHALNGYLFGNLPEPDMCVGESVSWHLFGMGNEIDIHSIYFYGNTFISRGHRTDVVNLFPATFLTTEMVVENPGKWMITCQVSDHLQAGMLGQYNVGNCKGDISHPKMKGQQRHYFIAAEKVLWDYAPQGYNKFSGLPLNASGSDSEFYFTQGDNRIGGKYWKAQYMEYVDATFTRRKRLSEAEAHLGILGPVIRAEVGDTLLVTFANKADKVYSILPHGVIYDKASDAAPNIDGFLKPGAHVKPGETFTYKWTVPESVSPTAGDPPCLTYLYFSAVEPIQDTSSGLVGPLLVCKKGVLNADGTQKGIDKEFYLLFTVFDENLSRYLDENIQRFTWRPFSVDKEDKDFVKSNRMHAINGYMYGNQPGLSMCKKDRVSWHMIGMGTDTDMHGGYFQGNTIHLQGTHRDTLALFPHFSTTVFMQPDHAGIFRVFCYTMHHFVRGMNQIYEVNSCGNKDPSEQPYGMIRTFYIAAEEVEWDYAPNKNWEFEKQHLDAGGERHGDIFMNHTENWIGSQYKKVVYREYTDGEFVEIKARPPREKHLELLGPMVHAEVGDSILIIFKNKAKRPYSISAHGIEDMDSGKQLHVPITKPGEINTYRWNVPKRSGPGPNDPNCIPWVYYSTVNFVKDTYSGLMGPLIICREGVLNEKGRRSDVDYEFVLLFLVFNENESWYLDDNIKKYLNKDPRYFKRSDDFEESNKMHAINGKIFGNLHGLIMNEDTMTNWYLLGIGSEVDIHTIHYHAESFLFKIDKSYREDVYDLFPGTFQTIELFADHPGTWLIHCHVSDHIHAGMETTYTVLRNIDNRIPYSTKSPSGGASPPATVPSNEEPGKEQLYFFGKNLHPRGAKAALVILFVIGLLFLISTVILSLRLHSAKKQVAYRQVQSCALPTDAL

Target ORF information:

RefSeq Version	XM_542245.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris hephaestin-like 1 (HEPHL1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_542245.5

ORF Insert Sequence:

ATGCCTCGGA AGCAGCAGGC TGACTGCATC GTTCTCTTCA CATTCCTGGG TCTGTCTGGG 
CTGGTTGGTG CAGTTACCAG AACATACCAC ATTGGGATTG TGGAAGAATA CTGGAACTAT 
GTACCCCAAG GGAAGAATGT TGTTACTGGG GGAAGTTTTT CAGAAGACAA GCTTGCAACC 
TTATTTCTCG AAAGAGGGCC CAACAGGATA GGTGGTCTTT ACAAAAAGGC TGTTTACAGA 
CATTATACAG ATGAGACGTA TTCCACAGAG ATTCCCAAAC CTCCCTGGCT CGGATTCCTG 
GGCCCTATCT TGAGGGCTGA AGTGGGTGAT GTGATTGTCA TTCATTTGAA GAACTTTGCT 
TCCCGACCCT ACTCTTTGCA TCCACATGGT GTTTTCTACA ACAAAGATTC AGAAGGAGCT 
CTATACCCAG ATGGAACATC CGGAAGGAAT AAGAATGATG ATATGGTTCC TCCTGGCAAA 
AACTACACCT ATGTCTGGCC AGTAAGAGAA GAATATGCCC CTGCTCCAGC AGATGCCAAC 
TGTCTGACCT GGGTGTACCA TTCACACATT GATGCTCCTA AGGATATCTG CTCTGGGTTA 
ATTGGCCCGC TCCTGGTGTG CAAGGAAGGT ATCCTGAATA AATATACAGG CACAAGGACA 
GATGTGGACC GAGAGTTCAT TATCATGTTT ACTCTGGTGG ATGAGAATCA AAGTTGGTAC 
CTTGATGAAA ATATCAGACA TTTCTGCACC AACCCTGACT CAGTTGACAA AAAAGATGCT 
GTTTTCCAGA GAAGTAACAA AATGCATGCC CTCAATGGAT ATCTCTTTGG AAACCTCCCT 
GAGCCTGATA TGTGTGTTGG TGAATCAGTG TCCTGGCACT TATTTGGGAT GGGGAATGAG 
ATAGACATCC ATTCCATCTA TTTCTATGGA AACACCTTCA TCAGCAGAGG GCATCGGACT 
GATGTCGTCA ACCTGTTCCC AGCCACCTTC CTGACAACAG AAATGGTGGT TGAGAATCCT 
GGGAAGTGGA TGATAACTTG CCAAGTCAGT GATCACCTAC AAGCTGGCAT GTTGGGACAG 
TACAATGTTG GTAACTGCAA AGGTGATATT TCTCATCCCA AGATGAAGGG TCAGCAGAGA 
CACTACTTTA TAGCAGCCGA AAAAGTTCTT TGGGATTATG CTCCTCAAGG CTATAACAAA 
TTCAGTGGTC TTCCCCTTAA TGCCTCTGGC AGTGACTCCG AGTTCTACTT CACACAAGGG 
GACAACAGAA TTGGAGGAAA ATATTGGAAG GCTCAGTATA TGGAGTATGT TGATGCAACT 
TTTACTCGAA GAAAGAGACT CTCTGAGGCA GAAGCCCATC TTGGAATTCT TGGCCCCGTC 
ATCAGGGCAG AGGTGGGTGA TACTCTGTTA GTGACCTTTG CCAACAAAGC CGACAAAGTC 
TACAGTATTT TGCCCCATGG CGTGATCTAT GACAAGGCGT CTGATGCAGC CCCAAACATA 
GATGGATTTC TGAAGCCAGG GGCGCATGTT AAGCCAGGTG AAACTTTCAC ATACAAGTGG 
ACGGTGCCTG AGAGTGTAAG CCCGACAGCT GGTGATCCCC CCTGTCTGAC CTATCTTTAC 
TTCTCAGCGG TTGAACCAAT CCAGGACACC AGCTCTGGCC TTGTGGGGCC TTTACTAGTC 
TGTAAAAAGG GCGTACTCAA TGCTGATGGG ACACAGAAAG GAATAGATAA GGAATTTTAC 
CTACTGTTCA CAGTCTTTGA TGAGAATCTG AGCAGGTACC TTGACGAAAA CATTCAGAGG 
TTTACATGGC GCCCCTTCAG TGTTGACAAA GAGGACAAGG ATTTTGTGAA GTCCAACCGA 
ATGCATGCGA TCAATGGCTA TATGTATGGC AACCAGCCAG GCCTAAGCAT GTGCAAAAAA 
GATAGAGTTT CCTGGCATAT GATTGGAATG GGCACCGACA CTGATATGCA TGGAGGTTAT 
TTTCAAGGGA ACACCATTCA CCTACAAGGG ACTCATCGCG ACACCCTAGC CCTGTTTCCG 
CATTTCTCTA CAACGGTGTT CATGCAGCCG GACCACGCAG GTATTTTCAG GGTGTTTTGT 
TACACCATGC ACCACTTCGT GAGAGGCATG AATCAAATAT ATGAGGTCAA CAGCTGTGGC 
AACAAGGACC CTTCTGAGCA GCCGTACGGG ATGATAAGAA CCTTTTACAT CGCTGCTGAA 
GAGGTAGAAT GGGATTATGC CCCTAACAAA AACTGGGAGT TCGAAAAGCA GCACTTGGAC 
GCAGGAGGGG AAAGACATGG AGATATATTT ATGAACCACA CTGAAAACTG GATTGGCTCT 
CAGTACAAGA AGGTAGTTTA CAGAGAATAC ACTGATGGAG AATTCGTGGA GATCAAGGCT 
CGACCACCAC GAGAGAAGCA CTTGGAACTC CTGGGCCCAA TGGTTCATGC TGAAGTGGGT 
GACTCCATCC TGATCATATT TAAGAACAAA GCCAAGCGGC CCTACTCCAT CTCAGCCCAT 
GGTATTGAAG ACATGGATAG TGGGAAGCAA CTCCATGTGC CCATCACAAA GCCAGGAGAA 
ATAAATACTT ACAGGTGGAA TGTCCCTAAA AGGTCTGGTC CAGGACCAAA TGACCCAAAT 
TGTATTCCAT GGGTTTACTA TTCAACAGTA AACTTTGTGA AGGATACATA CAGTGGTTTG 
ATGGGTCCTC TGATTATTTG CAGAGAAGGA GTGTTGAATG AGAAGGGAAG AAGAAGCGAT 
GTTGATTATG AATTCGTTCT CTTGTTTTTG GTATTTAATG AGAATGAATC CTGGTATCTG 
GATGACAATA TTAAAAAGTA TCTCAATAAA GATCCACGAT ATTTTAAGCG CAGTGACGAT 
TTTGAAGAAA GCAACAAAAT GCATGCTATC AATGGAAAGA TTTTTGGGAA TCTCCATGGC 
CTTATCATGA ATGAAGATAC GATGACAAAC TGGTATCTGT TGGGAATAGG AAGTGAAGTG 
GACATACACA CCATCCATTA TCATGCTGAG AGTTTTCTTT TCAAGATAGA TAAATCTTAC 
CGAGAAGATG TGTACGATCT CTTTCCTGGG ACATTCCAAA CCATTGAGCT GTTTGCAGAT 
CACCCAGGAA CATGGCTGAT ACACTGTCAT GTGTCTGACC ACATCCATGC TGGCATGGAG 
ACAACCTACA CAGTCCTTCG GAACATAGAC AACAGGATCC CTTACTCTAC CAAGTCTCCA 
TCTGGAGGAG CGTCCCCCCC AGCCACAGTG CCCTCCAATG AAGAACCCGG CAAGGAGCAG 
CTCTATTTCT TTGGCAAGAA TCTGCATCCT AGGGGAGCAA AAGCAGCCTT GGTCATCCTC 
TTCGTCATTG GACTCCTCTT CCTTATCTCC ACGGTGATTC TTTCCCTCAG ATTGCACTCT 
GCAAAGAAGC AGGTTGCATA TCGGCAAGTT CAGTCCTGCG CACTCCCCAC AGATGCTCTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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HEPHL1 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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