irs4a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	irs4a
Entrez Gene ID	100329897
Full Name	insulin receptor substrate 4a
Synonyms	si:ch73-362m14.1
General protein information	Preferred Names insulin receptor substrate 4a Names insulin receptor substrate 2-B
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002666075.5	XP_002666121.3	insulin receptor substrate 4
XM_002666075.6	XP_002666121.3	insulin receptor substrate 2-B

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	LOC422347	XP_003641132.2
Xenopus tropicalis (tropical clawed frog)	irs4	NP_001135469.1
Danio rerio (zebrafish)	irs4a	XP_002666121.3

Gene Ontology

The following irs4a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the irs4a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa26239	XM_002666075.6 Latest version!	Danio rerio insulin receptor substrate 4a (irs4a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
ODa26239	XM_002666075.5	Danio rerio insulin receptor substrate 4a (irs4a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

irs4a ( XM_002666075.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_002666121.3 Danio rerio insulin receptor substrate 4a (irs4a), mRNA.

Price & Availability↑

CloneID	ODa26239
Clone ID Related Accession (Same CDS sequence)	XM_002666075.5 , XM_002666075.6
Accession Version	XM_002666075.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3156bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	insulin receptor substrate 4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_002666075.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGATC GGCCGCGTTT ACCGAATCAC GGCGTCATGT TGACACTGGA GGCTCAACCG 
CGTCGCGCTG TCACGAAAAC ATCCGCGAGC GGCATCTCTT GTGCTGCTGC CGGGGAGCCT 
CCATCCTCCT CCTCCTCCTC CTCCTCCTCC TCCACCTCCA CCTCAATCAA TAACAGCGGC 
TGCCGTTTGC CCATGCACTT ATCAGCCTCC TCTCATTGCC AGCCACAGAG GAGGTACCAT 
CCGCCTTACC ACTTTAAAGT GCACCATCTG TTCCCGGATG AGAGCCCTCA GGATGCTGTG 
CTGAGGAAGA ACCTGCCAGC CCTACAGTAT AAGGTGCACG ACTCTGCCTT CTGCCGCGTG 
CTCAGCGCTG ACACCACTGC TGCCGCCCTG GCCGCTGCCG CCGCCTGCTC TGGTGGCCTG 
GACCGGGACA TCTGGAAGTG TGGCTATCTG CGAAAGCAGA AGCACGGCCA CAAGAGGTTC 
TTCGTATTGC GGGGCCCCAG CAACCTCGGG CCCAGCCGCT TGGAGTATTA CGACAGCGAG 
AAAAAATTTC GCAATGCCCT CAAAGCTGCT GCTTCTGGTG TCACGTGCCC TGCCAAAAGG 
GTGATTTATC TGTCGCAGTG TTTTACGGTT AACAAGAGGG CTGATGCTAA GAACAAATAC 
CTCATTGCCC TTTATACTAA AGACGAATAC TTTGCTGTTG TAGCCGAGAG CGAACAGGAG 
CAAGAAGAGT GGTACTTGTC TATAACGGAA CTTATGACGG AGGGCAAAAA GGGGCAGCTG 
GACAACGACG AACTTGATGA TGGCTATGGG ACGATTTCGC CGGGAACCAT TTTCAAGGAG 
GTGTGGCAGG TGAACGTTAA GCCTAAAGGC TTAGGTCAGA CTCGAAACTT AATGGGGGTG 
TACCGTCTGT GCCTTTCCGC CAAGACTATT CACCTGGTGA AGCTGAACTC GGAGACGCCT 
GCCGTCAACC TGCCGCTAAT GAACATTCGA CGCTGCGGTC ACTCCGAGAG CTTCTTCTTT 
ATCGAGGTGG GCCGCTCTTC TTCCATCGGC CCTGGCGAGA TATGGGTGCA AGTCGAGGAC 
TCTGTTGTGG CGCAGAACAT GCACGAGACC ATTCTAGACA CTATGAAGGC TCTGAAGGCC 
TTTCCTGACT TTAGGCCAAG GAGCAAAAGC CAGTCATCTG GCTCAAATCC GCTGCCGTTT 
ATTACGACTC GCCGGCATCT CGGCAATCTC CCCCCAAGCC AAACTGGCCT CCAGCGAGCC 
TCCCGAACGG ATTCAATGAC AGGCACGCCG CCCACAGGGA AAAGCAGAGG GCGGGGTCAG 
CGCTACCGCA CCTCTAGCGA GGGCGAAGGC ACTCAGGACC GCCCCCTCAG CTCTGGGACG 
GGCAGCTTGG TGCACATCAA TACCCCCATT CTCAGTGTGG GCCGGGAGGA AAGCGGCAGC 
TGCTGCGCTC ATGCATCACC CAATCAGGCC CATCACACCC GCTCCGCATC ACTTCCCGTA 
TCTCACTTCC TGTCTGCCAC CAGCCCGATC AGTGTGTCCA GCAGTAGTGG GCACGGCTCG 
GCTTCAGATA CACACACTCG ACCCTCAAGC TCCTCCATTT GTGGCTCCCC CAGCGACGGA 
GGCTTCAACT CCTCCGACGA GTTCTGCCCC AGCCCCTGCG ACTTCAGGTA CTTTCATGCA 
AGCTGTACCA CACCCGAATC CCACGGCAGC ACCCCGCCGA TTAGGGATGA CTACAGGCTG 
ACTGAGTATA TGACTATGGA TTGGCACAGA GATGGGGCGA GGACGTTCGA GGAGGAGAGC 
AGTTACATGG AGAGGACTTT CCTGAAGCTG GCACACTCGT CCAAGCCAAA GCTAGGCGGC 
AGCTTAAGCA TCACGCAGCA GAAAGCCACG CAGACCTCAT CGTCTTTTGA TGAACCGATG 
GAGTCTAAAC GGCAGCAGGT TTGCTCGTGC CTGCTGAAGT CAGCCTATAA GTCTTACTCT 
GAGTTACATC ACCCAAACAA GCCTCCCTTG CTCAGCTCTG AGTGTCAGAA GAAACTGCCA 
AAAGACGACG GCTACATGCC TATGATCTAC AGCATGGCTT CTTCTCCTAA TGCAGATTAC 
ACCCCTATGC AACCCCGAGC AAACCACCCT GCGTCTCTGG ACGTCCACCC CTGTTCCTCG 
CAGCAGTCGG ACCGGTACGG ATACATGATG ATGCTCCCTA GTGACAGCCC CTCGAGTCGG 
GAGCAGGCGG AGTACGACGA CTACATGGAT ATGTCACAGC TTACAATAGC TGAAGGCTTT 
TCTCAAGCCT CGCCCGATTC CCTCAAGGCC TCGTATTTCT CGCTTCCGCG CTCCTACAAA 
GCCCCGTCAC GAGAAAAGCA CGAAAAGGCC GAATACGTTC CCATGTCTCC AGCTGATCCC 
ATGAGTCCTC CTAGTCCCGG AGAGTATATT CACATGGACT TTGGTCCTCG TCTCGCTCAC 
CCCGCTCTGT CTCCACCCTC CGCAGACTCG CCCTCCACAC CTCCGCCCTC TCAGCCCTGC 
TACCGGATGT GTTGCTCCTC TCCAGACCAT GATTACTTGG GTCTAAACAT GGATAATCTC 
CTAAAGGACC GGCCTCCGCG ACACTCCCTC GTTGCTCCAT GGAATCCCCC TAATTACGCT 
CGCCCCTTAG GGTGCTCATA CGGACAGACC TCCGCTAATT TAGATCCCGA AAAGGTGCAC 
GGTGAATGCG GAGACGTTCC CTTAAGAACC ATGCAGCATC TTTGCATCTC CGAGCGACTG 
CCGTTCACTC ACACGGAGCC CAAAGTCATC AGGGCGGACC CGCAGGGGCG AAGGCGGCAC 
AGTTCGGAGA CTTTCATCCC CTCGCCGGCT ACCTTTGCTA GTGGTTGCGG AATCAGGCCC 
TCAGCCAATC AGAATCTCCC CGAAGCTAGC AGATGGCAGA GCTCCTTCGA TAGCATGTGG 
TCGCATCCGG ACGCCTCCCC GAACTCCGCC ACAGGTGGGA CGCCTCTGAA CACCTCATCC 
AGCTACCTCA ACTACATCGC TCTGGACCTG AGAGAAGTGG CCCGGGTCAC ACACGACGCT 
CCACGCCCAG CCCATAGCAA CCTCCAAGAG AGCGACGCTT ACGCTGGTAT AGACTTCTCC 
GTTTCGGGAG GACGTGCCAA TGCCTCAAAA GATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002666121.3
CDS	630..3785
Translation	MEDRPRLPNHGVMLTLEAQPRRAVTKTSASGISCAAAGEPPSSSSSSSSSSTSTSINNSGCRLPMHLSASSHCQPQRRYHPPYHFKVHHLFPDESPQDAVLRKNLPALQYKVHDSAFCRVLSADTTAAALAAAAACSGGLDRDIWKCGYLRKQKHGHKRFFVLRGPSNLGPSRLEYYDSEKKFRNALKAAASGVTCPAKRVIYLSQCFTVNKRADAKNKYLIALYTKDEYFAVVAESEQEQEEWYLSITELMTEGKKGQLDNDELDDGYGTISPGTIFKEVWQVNVKPKGLGQTRNLMGVYRLCLSAKTIHLVKLNSETPAVNLPLMNIRRCGHSESFFFIEVGRSSSIGPGEIWVQVEDSVVAQNMHETILDTMKALKAFPDFRPRSKSQSSGSNPLPFITTRRHLGNLPPSQTGLQRASRTDSMTGTPPTGKSRGRGQRYRTSSEGEGTQDRPLSSGTGSLVHINTPILSVGREESGSCCAHASPNQAHHTRSASLPVSHFLSATSPISVSSSSGHGSASDTHTRPSSSSICGSPSDGGFNSSDEFCPSPCDFRYFHASCTTPESHGSTPPIRDDYRLTEYMTMDWHRDGARTFEEESSYMERTFLKLAHSSKPKLGGSLSITQQKATQTSSSFDEPMESKRQQVCSCLLKSAYKSYSELHHPNKPPLLSSECQKKLPKDDGYMPMIYSMASSPNADYTPMQPRANHPASLDVHPCSSQQSDRYGYMMMLPSDSPSSREQAEYDDYMDMSQLTIAEGFSQASPDSLKASYFSLPRSYKAPSREKHEKAEYVPMSPADPMSPPSPGEYIHMDFGPRLAHPALSPPSADSPSTPPPSQPCYRMCCSSPDHDYLGLNMDNLLKDRPPRHSLVAPWNPPNYARPLGCSYGQTSANLDPEKVHGECGDVPLRTMQHLCISERLPFTHTEPKVIRADPQGRRRHSSETFIPSPATFASGCGIRPSANQNLPEASRWQSSFDSMWSHPDASPNSATGGTPLNTSSSYLNYIALDLREVARVTHDAPRPAHSNLQESDAYAGIDFSVSGGRANASKD

Target ORF information:

RefSeq Version	XM_002666075.5
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio insulin receptor substrate 4a (irs4a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002666075.5

ORF Insert Sequence:

ATGGAAGATC GGCCGCGTTT ACCGAATCAC GGCGTCATGT TGACACTGGA GGCTCAACCG 
CGTCGCGCTG TCACGAAAAC ATCCGCGAGC GGCATCTCTT GTGCTGCTGC CGGGGAGCCT 
CCATCCTCCT CCTCCTCCTC CTCCTCCTCC TCCACCTCCA CCTCAATCAA TAACAGCGGC 
TGCCGTTTGC CCATGCACTT ATCAGCCTCC TCTCATTGCC AGCCACAGAG GAGGTACCAT 
CCGCCTTACC ACTTTAAAGT GCACCATCTG TTCCCGGATG AGAGCCCTCA GGATGCTGTG 
CTGAGGAAGA ACCTGCCAGC CCTACAGTAT AAGGTGCACG ACTCTGCCTT CTGCCGCGTG 
CTCAGCGCTG ACACCACTGC TGCCGCCCTG GCCGCTGCCG CCGCCTGCTC TGGTGGCCTG 
GACCGGGACA TCTGGAAGTG TGGCTATCTG CGAAAGCAGA AGCACGGCCA CAAGAGGTTC 
TTCGTATTGC GGGGCCCCAG CAACCTCGGG CCCAGCCGCT TGGAGTATTA CGACAGCGAG 
AAAAAATTTC GCAATGCCCT CAAAGCTGCT GCTTCTGGTG TCACGTGCCC TGCCAAAAGG 
GTGATTTATC TGTCGCAGTG TTTTACGGTT AACAAGAGGG CTGATGCTAA GAACAAATAC 
CTCATTGCCC TTTATACTAA AGACGAATAC TTTGCTGTTG TAGCCGAGAG CGAACAGGAG 
CAAGAAGAGT GGTACTTGTC TATAACGGAA CTTATGACGG AGGGCAAAAA GGGGCAGCTG 
GACAACGACG AACTTGATGA TGGCTATGGG ACGATTTCGC CGGGAACCAT TTTCAAGGAG 
GTGTGGCAGG TGAACGTTAA GCCTAAAGGC TTAGGTCAGA CTCGAAACTT AATGGGGGTG 
TACCGTCTGT GCCTTTCCGC CAAGACTATT CACCTGGTGA AGCTGAACTC GGAGACGCCT 
GCCGTCAACC TGCCGCTAAT GAACATTCGA CGCTGCGGTC ACTCCGAGAG CTTCTTCTTT 
ATCGAGGTGG GCCGCTCTTC TTCCATCGGC CCTGGCGAGA TATGGGTGCA AGTCGAGGAC 
TCTGTTGTGG CGCAGAACAT GCACGAGACC ATTCTAGACA CTATGAAGGC TCTGAAGGCC 
TTTCCTGACT TTAGGCCAAG GAGCAAAAGC CAGTCATCTG GCTCAAATCC GCTGCCGTTT 
ATTACGACTC GCCGGCATCT CGGCAATCTC CCCCCAAGCC AAACTGGCCT CCAGCGAGCC 
TCCCGAACGG ATTCAATGAC AGGCACGCCG CCCACAGGGA AAAGCAGAGG GCGGGGTCAG 
CGCTACCGCA CCTCTAGCGA GGGCGAAGGC ACTCAGGACC GCCCCCTCAG CTCTGGGACG 
GGCAGCTTGG TGCACATCAA TACCCCCATT CTCAGTGTGG GCCGGGAGGA AAGCGGCAGC 
TGCTGCGCTC ATGCATCACC CAATCAGGCC CATCACACCC GCTCCGCATC ACTTCCCGTA 
TCTCACTTCC TGTCTGCCAC CAGCCCGATC AGTGTGTCCA GCAGTAGTGG GCACGGCTCG 
GCTTCAGATA CACACACTCG ACCCTCAAGC TCCTCCATTT GTGGCTCCCC CAGCGACGGA 
GGCTTCAACT CCTCCGACGA GTTCTGCCCC AGCCCCTGCG ACTTCAGGTA CTTTCATGCA 
AGCTGTACCA CACCCGAATC CCACGGCAGC ACCCCGCCGA TTAGGGATGA CTACAGGCTG 
ACTGAGTATA TGACTATGGA TTGGCACAGA GATGGGGCGA GGACGTTCGA GGAGGAGAGC 
AGTTACATGG AGAGGACTTT CCTGAAGCTG GCACACTCGT CCAAGCCAAA GCTAGGCGGC 
AGCTTAAGCA TCACGCAGCA GAAAGCCACG CAGACCTCAT CGTCTTTTGA TGAACCGATG 
GAGTCTAAAC GGCAGCAGGT TTGCTCGTGC CTGCTGAAGT CAGCCTATAA GTCTTACTCT 
GAGTTACATC ACCCAAACAA GCCTCCCTTG CTCAGCTCTG AGTGTCAGAA GAAACTGCCA 
AAAGACGACG GCTACATGCC TATGATCTAC AGCATGGCTT CTTCTCCTAA TGCAGATTAC 
ACCCCTATGC AACCCCGAGC AAACCACCCT GCGTCTCTGG ACGTCCACCC CTGTTCCTCG 
CAGCAGTCGG ACCGGTACGG ATACATGATG ATGCTCCCTA GTGACAGCCC CTCGAGTCGG 
GAGCAGGCGG AGTACGACGA CTACATGGAT ATGTCACAGC TTACAATAGC TGAAGGCTTT 
TCTCAAGCCT CGCCCGATTC CCTCAAGGCC TCGTATTTCT CGCTTCCGCG CTCCTACAAA 
GCCCCGTCAC GAGAAAAGCA CGAAAAGGCC GAATACGTTC CCATGTCTCC AGCTGATCCC 
ATGAGTCCTC CTAGTCCCGG AGAGTATATT CACATGGACT TTGGTCCTCG TCTCGCTCAC 
CCCGCTCTGT CTCCACCCTC CGCAGACTCG CCCTCCACAC CTCCGCCCTC TCAGCCCTGC 
TACCGGATGT GTTGCTCCTC TCCAGACCAT GATTACTTGG GTCTAAACAT GGATAATCTC 
CTAAAGGACC GGCCTCCGCG ACACTCCCTC GTTGCTCCAT GGAATCCCCC TAATTACGCT 
CGCCCCTTAG GGTGCTCATA CGGACAGACC TCCGCTAATT TAGATCCCGA AAAGGTGCAC 
GGTGAATGCG GAGACGTTCC CTTAAGAACC ATGCAGCATC TTTGCATCTC CGAGCGACTG 
CCGTTCACTC ACACGGAGCC CAAAGTCATC AGGGCGGACC CGCAGGGGCG AAGGCGGCAC 
AGTTCGGAGA CTTTCATCCC CTCGCCGGCT ACCTTTGCTA GTGGTTGCGG AATCAGGCCC 
TCAGCCAATC AGAATCTCCC CGAAGCTAGC AGATGGCAGA GCTCCTTCGA TAGCATGTGG 
TCGCATCCGG ACGCCTCCCC GAACTCCGCC ACAGGTGGGA CGCCTCTGAA CACCTCATCC 
AGCTACCTCA ACTACATCGC TCTGGACCTG AGAGAAGTGG CCCGGGTCAC ACACGACGCT 
CCACGCCCAG CCCATAGCAA CCTCCAAGAG AGCGACGCTT ACGCTGGTAT AGACTTCTCC 
GTTTCGGGAG GACGTGCCAA TGCCTCAAAA GATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

irs4a ( XM_002666075.6 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_002666121.3 Danio rerio insulin receptor substrate 4a (irs4a), mRNA.

Price & Availability↑

CloneID	ODa26239
Clone ID Related Accession (Same CDS sequence)	XM_002666075.5 , XM_002666075.6
Accession Version	XM_002666075.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3156bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	insulin receptor substrate 2-B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_002666075.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGATC GGCCGCGTTT ACCGAATCAC GGCGTCATGT TGACACTGGA GGCTCAACCG 
CGTCGCGCTG TCACGAAAAC ATCCGCGAGC GGCATCTCTT GTGCTGCTGC CGGGGAGCCT 
CCATCCTCCT CCTCCTCCTC CTCCTCCTCC TCCACCTCCA CCTCAATCAA TAACAGCGGC 
TGCCGTTTGC CCATGCACTT ATCAGCCTCC TCTCATTGCC AGCCACAGAG GAGGTACCAT 
CCGCCTTACC ACTTTAAAGT GCACCATCTG TTCCCGGATG AGAGCCCTCA GGATGCTGTG 
CTGAGGAAGA ACCTGCCAGC CCTACAGTAT AAGGTGCACG ACTCTGCCTT CTGCCGCGTG 
CTCAGCGCTG ACACCACTGC TGCCGCCCTG GCCGCTGCCG CCGCCTGCTC TGGTGGCCTG 
GACCGGGACA TCTGGAAGTG TGGCTATCTG CGAAAGCAGA AGCACGGCCA CAAGAGGTTC 
TTCGTATTGC GGGGCCCCAG CAACCTCGGG CCCAGCCGCT TGGAGTATTA CGACAGCGAG 
AAAAAATTTC GCAATGCCCT CAAAGCTGCT GCTTCTGGTG TCACGTGCCC TGCCAAAAGG 
GTGATTTATC TGTCGCAGTG TTTTACGGTT AACAAGAGGG CTGATGCTAA GAACAAATAC 
CTCATTGCCC TTTATACTAA AGACGAATAC TTTGCTGTTG TAGCCGAGAG CGAACAGGAG 
CAAGAAGAGT GGTACTTGTC TATAACGGAA CTTATGACGG AGGGCAAAAA GGGGCAGCTG 
GACAACGACG AACTTGATGA TGGCTATGGG ACGATTTCGC CGGGAACCAT TTTCAAGGAG 
GTGTGGCAGG TGAACGTTAA GCCTAAAGGC TTAGGTCAGA CTCGAAACTT AATGGGGGTG 
TACCGTCTGT GCCTTTCCGC CAAGACTATT CACCTGGTGA AGCTGAACTC GGAGACGCCT 
GCCGTCAACC TGCCGCTAAT GAACATTCGA CGCTGCGGTC ACTCCGAGAG CTTCTTCTTT 
ATCGAGGTGG GCCGCTCTTC TTCCATCGGC CCTGGCGAGA TATGGGTGCA AGTCGAGGAC 
TCTGTTGTGG CGCAGAACAT GCACGAGACC ATTCTAGACA CTATGAAGGC TCTGAAGGCC 
TTTCCTGACT TTAGGCCAAG GAGCAAAAGC CAGTCATCTG GCTCAAATCC GCTGCCGTTT 
ATTACGACTC GCCGGCATCT CGGCAATCTC CCCCCAAGCC AAACTGGCCT CCAGCGAGCC 
TCCCGAACGG ATTCAATGAC AGGCACGCCG CCCACAGGGA AAAGCAGAGG GCGGGGTCAG 
CGCTACCGCA CCTCTAGCGA GGGCGAAGGC ACTCAGGACC GCCCCCTCAG CTCTGGGACG 
GGCAGCTTGG TGCACATCAA TACCCCCATT CTCAGTGTGG GCCGGGAGGA AAGCGGCAGC 
TGCTGCGCTC ATGCATCACC CAATCAGGCC CATCACACCC GCTCCGCATC ACTTCCCGTA 
TCTCACTTCC TGTCTGCCAC CAGCCCGATC AGTGTGTCCA GCAGTAGTGG GCACGGCTCG 
GCTTCAGATA CACACACTCG ACCCTCAAGC TCCTCCATTT GTGGCTCCCC CAGCGACGGA 
GGCTTCAACT CCTCCGACGA GTTCTGCCCC AGCCCCTGCG ACTTCAGGTA CTTTCATGCA 
AGCTGTACCA CACCCGAATC CCACGGCAGC ACCCCGCCGA TTAGGGATGA CTACAGGCTG 
ACTGAGTATA TGACTATGGA TTGGCACAGA GATGGGGCGA GGACGTTCGA GGAGGAGAGC 
AGTTACATGG AGAGGACTTT CCTGAAGCTG GCACACTCGT CCAAGCCAAA GCTAGGCGGC 
AGCTTAAGCA TCACGCAGCA GAAAGCCACG CAGACCTCAT CGTCTTTTGA TGAACCGATG 
GAGTCTAAAC GGCAGCAGGT TTGCTCGTGC CTGCTGAAGT CAGCCTATAA GTCTTACTCT 
GAGTTACATC ACCCAAACAA GCCTCCCTTG CTCAGCTCTG AGTGTCAGAA GAAACTGCCA 
AAAGACGACG GCTACATGCC TATGATCTAC AGCATGGCTT CTTCTCCTAA TGCAGATTAC 
ACCCCTATGC AACCCCGAGC AAACCACCCT GCGTCTCTGG ACGTCCACCC CTGTTCCTCG 
CAGCAGTCGG ACCGGTACGG ATACATGATG ATGCTCCCTA GTGACAGCCC CTCGAGTCGG 
GAGCAGGCGG AGTACGACGA CTACATGGAT ATGTCACAGC TTACAATAGC TGAAGGCTTT 
TCTCAAGCCT CGCCCGATTC CCTCAAGGCC TCGTATTTCT CGCTTCCGCG CTCCTACAAA 
GCCCCGTCAC GAGAAAAGCA CGAAAAGGCC GAATACGTTC CCATGTCTCC AGCTGATCCC 
ATGAGTCCTC CTAGTCCCGG AGAGTATATT CACATGGACT TTGGTCCTCG TCTCGCTCAC 
CCCGCTCTGT CTCCACCCTC CGCAGACTCG CCCTCCACAC CTCCGCCCTC TCAGCCCTGC 
TACCGGATGT GTTGCTCCTC TCCAGACCAT GATTACTTGG GTCTAAACAT GGATAATCTC 
CTAAAGGACC GGCCTCCGCG ACACTCCCTC GTTGCTCCAT GGAATCCCCC TAATTACGCT 
CGCCCCTTAG GGTGCTCATA CGGACAGACC TCCGCTAATT TAGATCCCGA AAAGGTGCAC 
GGTGAATGCG GAGACGTTCC CTTAAGAACC ATGCAGCATC TTTGCATCTC CGAGCGACTG 
CCGTTCACTC ACACGGAGCC CAAAGTCATC AGGGCGGACC CGCAGGGGCG AAGGCGGCAC 
AGTTCGGAGA CTTTCATCCC CTCGCCGGCT ACCTTTGCTA GTGGTTGCGG AATCAGGCCC 
TCAGCCAATC AGAATCTCCC CGAAGCTAGC AGATGGCAGA GCTCCTTCGA TAGCATGTGG 
TCGCATCCGG ACGCCTCCCC GAACTCCGCC ACAGGTGGGA CGCCTCTGAA CACCTCATCC 
AGCTACCTCA ACTACATCGC TCTGGACCTG AGAGAAGTGG CCCGGGTCAC ACACGACGCT 
CCACGCCCAG CCCATAGCAA CCTCCAAGAG AGCGACGCTT ACGCTGGTAT AGACTTCTCC 
GTTTCGGGAG GACGTGCCAA TGCCTCAAAA GATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002666121.3
CDS	431..3586
Translation	MEDRPRLPNHGVMLTLEAQPRRAVTKTSASGISCAAAGEPPSSSSSSSSSSTSTSINNSGCRLPMHLSASSHCQPQRRYHPPYHFKVHHLFPDESPQDAVLRKNLPALQYKVHDSAFCRVLSADTTAAALAAAAACSGGLDRDIWKCGYLRKQKHGHKRFFVLRGPSNLGPSRLEYYDSEKKFRNALKAAASGVTCPAKRVIYLSQCFTVNKRADAKNKYLIALYTKDEYFAVVAESEQEQEEWYLSITELMTEGKKGQLDNDELDDGYGTISPGTIFKEVWQVNVKPKGLGQTRNLMGVYRLCLSAKTIHLVKLNSETPAVNLPLMNIRRCGHSESFFFIEVGRSSSIGPGEIWVQVEDSVVAQNMHETILDTMKALKAFPDFRPRSKSQSSGSNPLPFITTRRHLGNLPPSQTGLQRASRTDSMTGTPPTGKSRGRGQRYRTSSEGEGTQDRPLSSGTGSLVHINTPILSVGREESGSCCAHASPNQAHHTRSASLPVSHFLSATSPISVSSSSGHGSASDTHTRPSSSSICGSPSDGGFNSSDEFCPSPCDFRYFHASCTTPESHGSTPPIRDDYRLTEYMTMDWHRDGARTFEEESSYMERTFLKLAHSSKPKLGGSLSITQQKATQTSSSFDEPMESKRQQVCSCLLKSAYKSYSELHHPNKPPLLSSECQKKLPKDDGYMPMIYSMASSPNADYTPMQPRANHPASLDVHPCSSQQSDRYGYMMMLPSDSPSSREQAEYDDYMDMSQLTIAEGFSQASPDSLKASYFSLPRSYKAPSREKHEKAEYVPMSPADPMSPPSPGEYIHMDFGPRLAHPALSPPSADSPSTPPPSQPCYRMCCSSPDHDYLGLNMDNLLKDRPPRHSLVAPWNPPNYARPLGCSYGQTSANLDPEKVHGECGDVPLRTMQHLCISERLPFTHTEPKVIRADPQGRRRHSSETFIPSPATFASGCGIRPSANQNLPEASRWQSSFDSMWSHPDASPNSATGGTPLNTSSSYLNYIALDLREVARVTHDAPRPAHSNLQESDAYAGIDFSVSGGRANASKD

Target ORF information:

RefSeq Version	XM_002666075.6
Organism	Danio rerio(zebrafish)
Definition	Danio rerio insulin receptor substrate 4a (irs4a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002666075.6

ORF Insert Sequence:

ATGGAAGATC GGCCGCGTTT ACCGAATCAC GGCGTCATGT TGACACTGGA GGCTCAACCG 
CGTCGCGCTG TCACGAAAAC ATCCGCGAGC GGCATCTCTT GTGCTGCTGC CGGGGAGCCT 
CCATCCTCCT CCTCCTCCTC CTCCTCCTCC TCCACCTCCA CCTCAATCAA TAACAGCGGC 
TGCCGTTTGC CCATGCACTT ATCAGCCTCC TCTCATTGCC AGCCACAGAG GAGGTACCAT 
CCGCCTTACC ACTTTAAAGT GCACCATCTG TTCCCGGATG AGAGCCCTCA GGATGCTGTG 
CTGAGGAAGA ACCTGCCAGC CCTACAGTAT AAGGTGCACG ACTCTGCCTT CTGCCGCGTG 
CTCAGCGCTG ACACCACTGC TGCCGCCCTG GCCGCTGCCG CCGCCTGCTC TGGTGGCCTG 
GACCGGGACA TCTGGAAGTG TGGCTATCTG CGAAAGCAGA AGCACGGCCA CAAGAGGTTC 
TTCGTATTGC GGGGCCCCAG CAACCTCGGG CCCAGCCGCT TGGAGTATTA CGACAGCGAG 
AAAAAATTTC GCAATGCCCT CAAAGCTGCT GCTTCTGGTG TCACGTGCCC TGCCAAAAGG 
GTGATTTATC TGTCGCAGTG TTTTACGGTT AACAAGAGGG CTGATGCTAA GAACAAATAC 
CTCATTGCCC TTTATACTAA AGACGAATAC TTTGCTGTTG TAGCCGAGAG CGAACAGGAG 
CAAGAAGAGT GGTACTTGTC TATAACGGAA CTTATGACGG AGGGCAAAAA GGGGCAGCTG 
GACAACGACG AACTTGATGA TGGCTATGGG ACGATTTCGC CGGGAACCAT TTTCAAGGAG 
GTGTGGCAGG TGAACGTTAA GCCTAAAGGC TTAGGTCAGA CTCGAAACTT AATGGGGGTG 
TACCGTCTGT GCCTTTCCGC CAAGACTATT CACCTGGTGA AGCTGAACTC GGAGACGCCT 
GCCGTCAACC TGCCGCTAAT GAACATTCGA CGCTGCGGTC ACTCCGAGAG CTTCTTCTTT 
ATCGAGGTGG GCCGCTCTTC TTCCATCGGC CCTGGCGAGA TATGGGTGCA AGTCGAGGAC 
TCTGTTGTGG CGCAGAACAT GCACGAGACC ATTCTAGACA CTATGAAGGC TCTGAAGGCC 
TTTCCTGACT TTAGGCCAAG GAGCAAAAGC CAGTCATCTG GCTCAAATCC GCTGCCGTTT 
ATTACGACTC GCCGGCATCT CGGCAATCTC CCCCCAAGCC AAACTGGCCT CCAGCGAGCC 
TCCCGAACGG ATTCAATGAC AGGCACGCCG CCCACAGGGA AAAGCAGAGG GCGGGGTCAG 
CGCTACCGCA CCTCTAGCGA GGGCGAAGGC ACTCAGGACC GCCCCCTCAG CTCTGGGACG 
GGCAGCTTGG TGCACATCAA TACCCCCATT CTCAGTGTGG GCCGGGAGGA AAGCGGCAGC 
TGCTGCGCTC ATGCATCACC CAATCAGGCC CATCACACCC GCTCCGCATC ACTTCCCGTA 
TCTCACTTCC TGTCTGCCAC CAGCCCGATC AGTGTGTCCA GCAGTAGTGG GCACGGCTCG 
GCTTCAGATA CACACACTCG ACCCTCAAGC TCCTCCATTT GTGGCTCCCC CAGCGACGGA 
GGCTTCAACT CCTCCGACGA GTTCTGCCCC AGCCCCTGCG ACTTCAGGTA CTTTCATGCA 
AGCTGTACCA CACCCGAATC CCACGGCAGC ACCCCGCCGA TTAGGGATGA CTACAGGCTG 
ACTGAGTATA TGACTATGGA TTGGCACAGA GATGGGGCGA GGACGTTCGA GGAGGAGAGC 
AGTTACATGG AGAGGACTTT CCTGAAGCTG GCACACTCGT CCAAGCCAAA GCTAGGCGGC 
AGCTTAAGCA TCACGCAGCA GAAAGCCACG CAGACCTCAT CGTCTTTTGA TGAACCGATG 
GAGTCTAAAC GGCAGCAGGT TTGCTCGTGC CTGCTGAAGT CAGCCTATAA GTCTTACTCT 
GAGTTACATC ACCCAAACAA GCCTCCCTTG CTCAGCTCTG AGTGTCAGAA GAAACTGCCA 
AAAGACGACG GCTACATGCC TATGATCTAC AGCATGGCTT CTTCTCCTAA TGCAGATTAC 
ACCCCTATGC AACCCCGAGC AAACCACCCT GCGTCTCTGG ACGTCCACCC CTGTTCCTCG 
CAGCAGTCGG ACCGGTACGG ATACATGATG ATGCTCCCTA GTGACAGCCC CTCGAGTCGG 
GAGCAGGCGG AGTACGACGA CTACATGGAT ATGTCACAGC TTACAATAGC TGAAGGCTTT 
TCTCAAGCCT CGCCCGATTC CCTCAAGGCC TCGTATTTCT CGCTTCCGCG CTCCTACAAA 
GCCCCGTCAC GAGAAAAGCA CGAAAAGGCC GAATACGTTC CCATGTCTCC AGCTGATCCC 
ATGAGTCCTC CTAGTCCCGG AGAGTATATT CACATGGACT TTGGTCCTCG TCTCGCTCAC 
CCCGCTCTGT CTCCACCCTC CGCAGACTCG CCCTCCACAC CTCCGCCCTC TCAGCCCTGC 
TACCGGATGT GTTGCTCCTC TCCAGACCAT GATTACTTGG GTCTAAACAT GGATAATCTC 
CTAAAGGACC GGCCTCCGCG ACACTCCCTC GTTGCTCCAT GGAATCCCCC TAATTACGCT 
CGCCCCTTAG GGTGCTCATA CGGACAGACC TCCGCTAATT TAGATCCCGA AAAGGTGCAC 
GGTGAATGCG GAGACGTTCC CTTAAGAACC ATGCAGCATC TTTGCATCTC CGAGCGACTG 
CCGTTCACTC ACACGGAGCC CAAAGTCATC AGGGCGGACC CGCAGGGGCG AAGGCGGCAC 
AGTTCGGAGA CTTTCATCCC CTCGCCGGCT ACCTTTGCTA GTGGTTGCGG AATCAGGCCC 
TCAGCCAATC AGAATCTCCC CGAAGCTAGC AGATGGCAGA GCTCCTTCGA TAGCATGTGG 
TCGCATCCGG ACGCCTCCCC GAACTCCGCC ACAGGTGGGA CGCCTCTGAA CACCTCATCC 
AGCTACCTCA ACTACATCGC TCTGGACCTG AGAGAAGTGG CCCGGGTCAC ACACGACGCT 
CCACGCCCAG CCCATAGCAA CCTCCAAGAG AGCGACGCTT ACGCTGGTAT AGACTTCTCC 
GTTTCGGGAG GACGTGCCAA TGCCTCAAAA GATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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irs4a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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