IRS4 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	IRS4
Entrez Gene ID	422347
Full Name	insulin receptor substrate 4
General protein information	Preferred Names insulin receptor substrate 4 Names insulin receptor substrate 4
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003641084.3	XP_003641132.3	insulin receptor substrate 4
XM_003641084.4	XP_003641132.3	insulin receptor substrate 4

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	LOC422347	XP_003641132.2
Xenopus tropicalis (tropical clawed frog)	irs4	NP_001135469.1
Danio rerio (zebrafish)	irs4a	XP_002666121.3

Gene Ontology

The following IRS4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IRS4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa150394	XM_003641084.4 Latest version!	Gallus gallus insulin receptor substrate 4 (IRS4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OGa34858	XM_003641084.3 Latest version!	Gallus gallus insulin receptor substrate 4 (IRS4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IRS4 ( XM_003641084.4 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_003641132.3 Gallus gallus insulin receptor substrate 4 (IRS4), mRNA.

Price & Availability↑

CloneID	OGa150394
Clone ID Related Accession (Same CDS sequence)	XM_003641084.4
Accession Version	XM_003641084.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	insulin receptor substrate 4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_003641084.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCG GCATGAACGG CCCGGGCGGC GGCGGCGGCG CCGGCGCGGC GGTCAGGGGC 
GGCGAGGAGG AGCCGGGCGC CCGGCACGCA GGAGGCGGAG CCGTGCCTCA GCAGCGGGAA 
CCCGGTGCGG CGGGCGAGGG GGAGCCGGCG GCGGGGGACG GTGGGCGCTG CCCGTCCCCG 
CAGCCCCACC ACCTGCTGCT GCTGCTGCGG CGCTCTCCCA GCGCTTCGCT CTGTCCGCCC 
GCGGAGGGCC CCGCGGGCCG CGCCGCCCTG GGCCGCGGCG GGCAGCCCCC CCCTGCGGGC 
CGCGCCGCAC CCCCGGCCTC GTCCTCCGGC GGCGACGACG TGAGGAAGTG CGGCTACCTG 
AGGAAGCAGA AGCACGGGCA CAAGCGGTAC TTCGTGCTGC GGGCCGATAG CCCGCTGGCC 
CCTGCCCGCC TGGAGTACTA CGACAGCGAG AAGAAGTTCA AGAGCAGCCT GCGGGCGGGG 
GGCGGCGGGG CGGCCCCTGT CTGCTGCCCA CCGCCCAAGC GGGTCATCCC GCTCTACCAG 
TGCTTCACCG TCAGCCGGCG CGCCGATGCC AAGCACAAGC ACATCATTGC CTTGTACACC 
AAGGATGAGT ACTTTGCTAT GCTGGCGGAG AATGAGGCCG AGCAGGAGGC CTGGTACGAA 
GCCATCAGCG AGCTGATGAG CCAGACCAAG AGGGGCTGCC TGGAACAGGA GGACCAGGCG 
GAGCAGCAGG TGGATGAGGA CGACGAGCAC TACGGGACCT CCCTGAGGCC TGGCACTGTC 
TTCAAGGAGG TGTGGCAGGT TAACGTGAAG CCCAAAGGGC TGGGCCAGAC GAAAAACCTC 
ACTGGAGTGT ATCGGCTGTG CCTCTCCAGC AAGGCTATCC ACCTTGTTAA GCTTAACTCG 
GAGGTGCCCT CTGTCCACCT ACAGCTGATG AATATTCGCC GCTGTGGGCA CTCAGAGAAC 
TTCTTCTTCA TCGAAGTGGG CAGATCAGCC TCCATCGGGC CTGGAGAGCT CTGGATGCAA 
GTGGATGATT CTGTGGTGGC CCAGAATATG CACGAGACTT TTTTGGAGAC CATGAAAGCG 
CTCAAAGCCT TTGCAGAGTT TAGGCCCCGA AGCAAAAGCC AGTCTTCTGG TGGTGGTGGT 
GGTACCAATC CCATCTCCTT CATCACCACT AGGAGGCACC TGGGCAACCT GCCCCCCAGC 
CAGACGGGCT TACAGAGAAG ATCGAGAACT GAGAGTGTAG TTGGAGGGAC GCCTCCTACC 
ACCAAGAGCA GCAACTCCTA CCGCTTCAGG ACATCCAGTG AGGGAGAAGG AACCATGACT 
AGACCTTTCA GGTCAGTGAC AGGGAGCCTG ATCCACCTCA ACACTGCAAG GATGAATTTG 
GGCCGGCAAG AAGGGAGTGG AAGGTACGTT AGAGCTGCAT TCAGCTCATC ATATCACACT 
AGGTCTGCTT CGCTGCCTGT GTCTCATTTT CCCTCCACTA CGAGTCCTAT CAGCGTTTCT 
TCCAGCAGTG GCCATGGCTC TGCTTCAGAC ATGTTGACCA GGCCTTCTAG CTCGTCTGTT 
TGTGGCTCTC CAAGTGATGG GGGATTTATC TCTTCTGATG AGTATGGCTC CAGCCCTGGA 
GATTTCAGGT ACTTTAGGGT CCGGAGTAAT ACACCGGATT CCTTGGGAAA CACACCGCCT 
ATCAGAGAGG AGAATTGTCT GAGTGAGTAC ATGTCCATGA GTAAGCAGCA AGCAGACGAT 
AACTCAAGAG ATGATTATAT GGAGGCTGAA AAGTGTTTCA GAAAAAGAAC TTACTCTCTA 
ACTAAACCAA CTTCGGTAGC AGTGCAGCAG AAAACAACAC AAACTACAGC TTTGTTAGAT 
GAAGATTCCG TAGGAAATCA TGGACGATTA CTTTACTCTG AAACATCAAA ATTGAAAGGT 
AACCATGAAT TGGAGTACAG TGACAGTAAT CTTGATTCTA TATGCAACCA AAGCAGGAGT 
AAAGCCAGGG ATGATGGATA CATGCCAATG ATGCCAGGCG TTGCGTCCTC TCTGTCAAGT 
AACAGCGATT ATTTGCCAAT GACTCCTAAA AGTATGTCTG TTCCGAAGCA GATTAACAAT 
TCATGGTCAC CATCTCAGGT CGACTCAAGA GGATATATGA TGATGTTTCC AAAGGCAAGC 
TCTTCACCTG TACGGAGTCC TTTAACTGGA TTTATTTCTA AAGGTAGTAA CGAGAAGATT 
GTCAACAATG AATACATGGA TATGTCACCT GGTAATTCAG CTCCAAAGCA TCCCGGTGAT 
TCAAATTATA TACATACTAG TTCTGCCTCC AAAGGTTTCA GTTCATATTT TTCTTTGCCT 
CGAAGCTTTA AGGCATTAAC AGGACAAAAT GGTGACCACA GTGAATATGT TCCAATGTCT 
TCACCTGGAA AACTCCTGTA TGGTGGACCA GAAAATGTAA AAAGCACCAA CAGCGAAGTT 
TTGTCTAACG GCATCTCTAA GTCACCGGTG GCAAAAGGTT CAGATGAAGG ACTGGTGCAG 
AACAGGGCTA CACGGCCAAC AAGACTCCCC CTCGGTACAA GAGGGAGTAA TACTATCCCA 
AGAATGTATG ATCGCACAGT TCCACCTGAG CCGGCAAGTC CTGGTGAATA CATCAATATT 
GATTTTAATG AGAAGGCAAG TAATACACCG TATTCCTTAT CTGCAGAAGG ATCGCCATCA 
TCTCTGGGCT CAAGTAGTGA CCACAGGCAG TCCCCACTCT CAGATTATAT GAGCGTAGAC 
TTGGATGTAC AGTCACCAAA AGTAGCGAAA GAACTATCTA ACTCTTTAAC AGATATTTCA 
ATTTATGCAA GTTCCAGTAT TCCTAGAAAC CAACAAAATC CTGACTATGC TAGGCTCTCC 
TTTGGTACTG CATGTGTTAG CGCTGCGAAT AACAGGACTG ATGATTACAC AGAGATGACA 
TTCAACATGG CAGCAACGCC ACCTCGACCC TTTGCTGCCG AATCCGATGA TGGTGTAAAG 
ATGGATAGTC CTTCTTCCAT AGTTAACAGA CTGTGCATTG TTGATCGATA TGCTGGTAGC 
AGTAGTTTTT CTGTTCCTAG TGCTGAGCCT CCAATGGGAC CGAAAGTTAT CCGAGCTGAT 
CCTCAAGGCA GGAGGAGACA CAGTTCTGAA ACCTTCTCTT CTGCTGGGAC TGTGACGACA 
TCCTCCTCTT TCTTTACTGA TAGTAGCAAA AGACACAGCT CTGCCTCATT TGATAATGTT 
TGGTTAAAAC CAGATGAAAG CATTTCTGAT GGTCAGGAAA GCAAAATGTC CAGGGATACC 
TCAACTGGAT TTCAAAATGG CTTAAACTAC ATAGCTCTGA ATTTATGCGA TGATCCAATA 
AGCTGTGAGG CAAGTACTGG GACACCAACT TGCCATCTCC AAAATGGTAC TTCAAGTTTG 
GACAGTGGAG CGTATGTAAG CATAGACTTC ACCAGGTCTG ATGGTCTGAA GTGTAACGCT 
GCACGAAAAG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003641132.3
CDS	279..3773
Translation	MASGMNGPGGGGGAGAAVRGGEEEPGARHAGGGAVPQQREPGAAGEGEPAAGDGGRCPSPQPHHLLLLLRRSPSASLCPPAEGPAGRAALGRGGQPPPAGRAAPPASSSGGDDVRKCGYLRKQKHGHKRYFVLRADSPLAPARLEYYDSEKKFKSSLRAGGGGAAPVCCPPPKRVIPLYQCFTVSRRADAKHKHIIALYTKDEYFAMLAENEAEQEAWYEAISELMSQTKRGCLEQEDQAEQQVDEDDEHYGTSLRPGTVFKEVWQVNVKPKGLGQTKNLTGVYRLCLSSKAIHLVKLNSEVPSVHLQLMNIRRCGHSENFFFIEVGRSASIGPGELWMQVDDSVVAQNMHETFLETMKALKAFAEFRPRSKSQSSGGGGGTNPISFITTRRHLGNLPPSQTGLQRRSRTESVVGGTPPTTKSSNSYRFRTSSEGEGTMTRPFRSVTGSLIHLNTARMNLGRQEGSGRYVRAAFSSSYHTRSASLPVSHFPSTTSPISVSSSSGHGSASDMLTRPSSSSVCGSPSDGGFISSDEYGSSPGDFRYFRVRSNTPDSLGNTPPIREENCLSEYMSMSKQQADDNSRDDYMEAEKCFRKRTYSLTKPTSVAVQQKTTQTTALLDEDSVGNHGRLLYSETSKLKGNHELEYSDSNLDSICNQSRSKARDDGYMPMMPGVASSLSSNSDYLPMTPKSMSVPKQINNSWSPSQVDSRGYMMMFPKASSSPVRSPLTGFISKGSNEKIVNNEYMDMSPGNSAPKHPGDSNYIHTSSASKGFSSYFSLPRSFKALTGQNGDHSEYVPMSSPGKLLYGGPENVKSTNSEVLSNGISKSPVAKGSDEGLVQNRATRPTRLPLGTRGSNTIPRMYDRTVPPEPASPGEYINIDFNEKASNTPYSLSAEGSPSSLGSSSDHRQSPLSDYMSVDLDVQSPKVAKELSNSLTDISIYASSSIPRNQQNPDYARLSFGTACVSAANNRTDDYTEMTFNMAATPPRPFAAESDDGVKMDSPSSIVNRLCIVDRYAGSSSFSVPSAEPPMGPKVIRADPQGRRRHSSETFSSAGTVTTSSSFFTDSSKRHSSASFDNVWLKPDESISDGQESKMSRDTSTGFQNGLNYIALNLCDDPISCEASTGTPTCHLQNGTSSLDSGAYVSIDFTRSDGLKCNAARKD

Target ORF information:

RefSeq Version	XM_003641084.4
Organism	Gallus gallus(chicken)
Definition	Gallus gallus insulin receptor substrate 4 (IRS4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003641084.4

ORF Insert Sequence:

ATGGCGAGCG GCATGAACGG CCCGGGCGGC GGCGGCGGCG CCGGCGCGGC GGTCAGGGGC 
GGCGAGGAGG AGCCGGGCGC CCGGCACGCA GGAGGCGGAG CCGTGCCTCA GCAGCGGGAA 
CCCGGTGCGG CGGGCGAGGG GGAGCCGGCG GCGGGGGACG GTGGGCGCTG CCCGTCCCCG 
CAGCCCCACC ACCTGCTGCT GCTGCTGCGG CGCTCTCCCA GCGCTTCGCT CTGTCCGCCC 
GCGGAGGGCC CCGCGGGCCG CGCCGCCCTG GGCCGCGGCG GGCAGCCCCC CCCTGCGGGC 
CGCGCCGCAC CCCCGGCCTC GTCCTCCGGC GGCGACGACG TGAGGAAGTG CGGCTACCTG 
AGGAAGCAGA AGCACGGGCA CAAGCGGTAC TTCGTGCTGC GGGCCGATAG CCCGCTGGCC 
CCTGCCCGCC TGGAGTACTA CGACAGCGAG AAGAAGTTCA AGAGCAGCCT GCGGGCGGGG 
GGCGGCGGGG CGGCCCCTGT CTGCTGCCCA CCGCCCAAGC GGGTCATCCC GCTCTACCAG 
TGCTTCACCG TCAGCCGGCG CGCCGATGCC AAGCACAAGC ACATCATTGC CTTGTACACC 
AAGGATGAGT ACTTTGCTAT GCTGGCGGAG AATGAGGCCG AGCAGGAGGC CTGGTACGAA 
GCCATCAGCG AGCTGATGAG CCAGACCAAG AGGGGCTGCC TGGAACAGGA GGACCAGGCG 
GAGCAGCAGG TGGATGAGGA CGACGAGCAC TACGGGACCT CCCTGAGGCC TGGCACTGTC 
TTCAAGGAGG TGTGGCAGGT TAACGTGAAG CCCAAAGGGC TGGGCCAGAC GAAAAACCTC 
ACTGGAGTGT ATCGGCTGTG CCTCTCCAGC AAGGCTATCC ACCTTGTTAA GCTTAACTCG 
GAGGTGCCCT CTGTCCACCT ACAGCTGATG AATATTCGCC GCTGTGGGCA CTCAGAGAAC 
TTCTTCTTCA TCGAAGTGGG CAGATCAGCC TCCATCGGGC CTGGAGAGCT CTGGATGCAA 
GTGGATGATT CTGTGGTGGC CCAGAATATG CACGAGACTT TTTTGGAGAC CATGAAAGCG 
CTCAAAGCCT TTGCAGAGTT TAGGCCCCGA AGCAAAAGCC AGTCTTCTGG TGGTGGTGGT 
GGTACCAATC CCATCTCCTT CATCACCACT AGGAGGCACC TGGGCAACCT GCCCCCCAGC 
CAGACGGGCT TACAGAGAAG ATCGAGAACT GAGAGTGTAG TTGGAGGGAC GCCTCCTACC 
ACCAAGAGCA GCAACTCCTA CCGCTTCAGG ACATCCAGTG AGGGAGAAGG AACCATGACT 
AGACCTTTCA GGTCAGTGAC AGGGAGCCTG ATCCACCTCA ACACTGCAAG GATGAATTTG 
GGCCGGCAAG AAGGGAGTGG AAGGTACGTT AGAGCTGCAT TCAGCTCATC ATATCACACT 
AGGTCTGCTT CGCTGCCTGT GTCTCATTTT CCCTCCACTA CGAGTCCTAT CAGCGTTTCT 
TCCAGCAGTG GCCATGGCTC TGCTTCAGAC ATGTTGACCA GGCCTTCTAG CTCGTCTGTT 
TGTGGCTCTC CAAGTGATGG GGGATTTATC TCTTCTGATG AGTATGGCTC CAGCCCTGGA 
GATTTCAGGT ACTTTAGGGT CCGGAGTAAT ACACCGGATT CCTTGGGAAA CACACCGCCT 
ATCAGAGAGG AGAATTGTCT GAGTGAGTAC ATGTCCATGA GTAAGCAGCA AGCAGACGAT 
AACTCAAGAG ATGATTATAT GGAGGCTGAA AAGTGTTTCA GAAAAAGAAC TTACTCTCTA 
ACTAAACCAA CTTCGGTAGC AGTGCAGCAG AAAACAACAC AAACTACAGC TTTGTTAGAT 
GAAGATTCCG TAGGAAATCA TGGACGATTA CTTTACTCTG AAACATCAAA ATTGAAAGGT 
AACCATGAAT TGGAGTACAG TGACAGTAAT CTTGATTCTA TATGCAACCA AAGCAGGAGT 
AAAGCCAGGG ATGATGGATA CATGCCAATG ATGCCAGGCG TTGCGTCCTC TCTGTCAAGT 
AACAGCGATT ATTTGCCAAT GACTCCTAAA AGTATGTCTG TTCCGAAGCA GATTAACAAT 
TCATGGTCAC CATCTCAGGT CGACTCAAGA GGATATATGA TGATGTTTCC AAAGGCAAGC 
TCTTCACCTG TACGGAGTCC TTTAACTGGA TTTATTTCTA AAGGTAGTAA CGAGAAGATT 
GTCAACAATG AATACATGGA TATGTCACCT GGTAATTCAG CTCCAAAGCA TCCCGGTGAT 
TCAAATTATA TACATACTAG TTCTGCCTCC AAAGGTTTCA GTTCATATTT TTCTTTGCCT 
CGAAGCTTTA AGGCATTAAC AGGACAAAAT GGTGACCACA GTGAATATGT TCCAATGTCT 
TCACCTGGAA AACTCCTGTA TGGTGGACCA GAAAATGTAA AAAGCACCAA CAGCGAAGTT 
TTGTCTAACG GCATCTCTAA GTCACCGGTG GCAAAAGGTT CAGATGAAGG ACTGGTGCAG 
AACAGGGCTA CACGGCCAAC AAGACTCCCC CTCGGTACAA GAGGGAGTAA TACTATCCCA 
AGAATGTATG ATCGCACAGT TCCACCTGAG CCGGCAAGTC CTGGTGAATA CATCAATATT 
GATTTTAATG AGAAGGCAAG TAATACACCG TATTCCTTAT CTGCAGAAGG ATCGCCATCA 
TCTCTGGGCT CAAGTAGTGA CCACAGGCAG TCCCCACTCT CAGATTATAT GAGCGTAGAC 
TTGGATGTAC AGTCACCAAA AGTAGCGAAA GAACTATCTA ACTCTTTAAC AGATATTTCA 
ATTTATGCAA GTTCCAGTAT TCCTAGAAAC CAACAAAATC CTGACTATGC TAGGCTCTCC 
TTTGGTACTG CATGTGTTAG CGCTGCGAAT AACAGGACTG ATGATTACAC AGAGATGACA 
TTCAACATGG CAGCAACGCC ACCTCGACCC TTTGCTGCCG AATCCGATGA TGGTGTAAAG 
ATGGATAGTC CTTCTTCCAT AGTTAACAGA CTGTGCATTG TTGATCGATA TGCTGGTAGC 
AGTAGTTTTT CTGTTCCTAG TGCTGAGCCT CCAATGGGAC CGAAAGTTAT CCGAGCTGAT 
CCTCAAGGCA GGAGGAGACA CAGTTCTGAA ACCTTCTCTT CTGCTGGGAC TGTGACGACA 
TCCTCCTCTT TCTTTACTGA TAGTAGCAAA AGACACAGCT CTGCCTCATT TGATAATGTT 
TGGTTAAAAC CAGATGAAAG CATTTCTGAT GGTCAGGAAA GCAAAATGTC CAGGGATACC 
TCAACTGGAT TTCAAAATGG CTTAAACTAC ATAGCTCTGA ATTTATGCGA TGATCCAATA 
AGCTGTGAGG CAAGTACTGG GACACCAACT TGCCATCTCC AAAATGGTAC TTCAAGTTTG 
GACAGTGGAG CGTATGTAAG CATAGACTTC ACCAGGTCTG ATGGTCTGAA GTGTAACGCT 
GCACGAAAAG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IRS4 ( XM_003641084.3 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_003641132.3 Gallus gallus insulin receptor substrate 4 (IRS4), mRNA.

Price & Availability↑

CloneID	OGa34858
Clone ID Related Accession (Same CDS sequence)	XM_003641084.3
Accession Version	XM_003641084.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1451750400000
Organism	Gallus gallus(chicken)
Product	insulin receptor substrate 4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763735.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_003641084.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGCG GCATGAACGG CCCGGGCGGC GGCGGCGGCG CCGGCGCGGC GGTCAGGGGC 
GGCGAGGAGG AGCCGGGCGC CCGGCACGCA GGAGGCGGAG CCGTGCCTCA GCAGCGGGAA 
CCCGGTGCGG CGGGCGAGGG GGAGCCGGCG GCGGGGGACG GTGGGCGCTG CCCGTCCCCG 
CAGCCCCACC ACCTGCTGCT GCTGCTGCGG CGCTCTCCCA GCGCTTCGCT CTGTCCGCCC 
GCGGAGGGCC CCGCGGGCCG CGCCGCCCTG GGCCGCGGCG GGCAGCCCCC CCCTGCGGGC 
CGCGCCGCAC CCCCGGCCTC GTCCTCCGGC GGCGACGACG TGAGGAAGTG CGGCTACCTG 
CGGAAGCAGA AGCACGGGCA CAAGCGGTAC TTCGTGCTGC GGGCCGATAG CCCGCTGGCC 
CCTGCCCGCC TGGAGTACTA CGACAGCGAG AAGAAGTTCA AGAGCAGCCT GCGGGCGGGG 
GGCGGCGGGG CGGCCCCTGT CTGCTGCCCA CCGCCCAAGC GGGTCATCCC GCTCTACCAG 
TGCTTCACCG TCAGCCGGCG CGCCGATGCC AAGCACAAGC ACATCATTGC CTTGTACACC 
AAGGATGAGT ACTTTGCTAT GCTGGCGGAG AATGAGGCCG AGCAGGAGGC CTGGTACGAA 
GCCATCAGCG AGCTGATGAG CCAGACCAAG AGGGGCTGCC TGGAACAGGA GGACCAGGCG 
GAGCAGCAGG TGGATGAGGA CGACGAGCAC TACGGGACCT CCCTGAGGCC TGGCACTGTC 
TTCAAGGAGG TGTGGCAGGT TAACGTGAAG CCCAAAGGGC TGGGCCAGAC GAAAAACCTC 
ACTGGAGTGT ATCGGCTGTG CCTCTCCAGC AAGGCTATCC ACCTTGTTAA GCTTAACTCG 
GAGGTGCCCT CTGTCCACCT ACAGCTGATG AATATTCGCC GCTGTGGGCA CTCAGAGAAC 
TTCTTCTTCA TCGAAGTGGG CAGATCAGCC TCCATCGGGC CTGGAGAGCT CTGGATGCAA 
GTGGATGATT CTGTGGTGGC CCAGAATATG CACGAGACTT TTTTGGAGAC CATGAAAGCG 
CTCAAAGCCT TTGCAGAGTT TAGGCCCCGA AGCAAAAGCC AGTCTTCTGG TGGTGGTGGT 
GGTACCAATC CCATCTCCTT CATCACCACT AGGAGGCACC TGGGCAACCT GCCCCCCAGC 
CAGACGGGCT TACAGAGAAG ATCGAGAACT GAGAGTGTAG TTGGAGGGAC GCCTCCTACC 
ACCAAGAGCA GCAACTCCTA CCGCTTCAGG ACATCCAGTG AGGGAGAAGG AACCATGACT 
AGACCTTTCA GGTCAGTGAC AGGGAGCCTG ATCCACCTCA ACACTGCAAG GATGAATTTG 
GGCCGGCAAG AAGGGAGTGG AAGGTACGTT AGAGCTGCAT TCAGCTCATC ATATCACACT 
AGGTCTGCTT CGCTGCCTGT GTCTCATTTT CCCTCCACTA CGAGTCCTAT CAGCGTTTCT 
TCCAGCAGTG GCCATGGCTC TGCTTCAGAC ATGTTGACCA GGCCTTCTAG CTCGTCTGTT 
TGTGGCTCTC CAAGTGATGG GGGATTTATC TCTTCTGATG AGTATGGCTC CAGCCCTGGA 
GATTTCAGGT ACTTTAGGGT CCGGAGTAAT ACACCGGATT CCTTGGGAAA CACACCGCCT 
ATCAGAGAGG AGAATTGTCT GAGTGAGTAC ATGTCCATGA GTAAGCAGCA AGCAGACGAT 
AACTCAAGAG ATGATTATAT GGAGGCTGAA AAGTGTTTCA GAAAAAGAAC TTACTCTCTA 
ACTAAACCAA CTTCGGTAGC AGTGCAGCAG AAAACAACAC AAACTACAGC TTTGTTAGAT 
GAAGATTCCG TAGGAAATCA TGGACGATTA CTTTACTCTG AAACATCAAA ATTGAAAGGT 
AACCATGAAT TGGAGTACAG TGACAGTAAT CTTGATTCTA TATGCAACCA AAGCAGGAGT 
AAAGCCAGGG ATGATGGATA CATGCCAATG ATGCCAGGCG TTGCGTCCTC TCTGTCAAGT 
AACAGCGATT ATTTGCCAAT GACTCCTAAA AGTATGTCTG TTCCGAAGCA GATTAACAAT 
TCATGGTCAC CATCTCAGGT CGACTCAAGA GGATATATGA TGATGTTTCC AAAGGCAAGC 
TCTTCACCTG TACGGAGTCC TTTAACTGGA TTTATTTCTA AAGGTAGTAA CGAGAAGATT 
GTCAACAATG AATACATGGA TATGTCACCT GGTAATTCAG CTCCAAAGCA TCCCGGTGAT 
TCAAATTATA TACATACTAG TTCTGCCTCC AAAGGTTTCA GTTCATATTT TTCTTTGCCT 
CGAAGCTTTA AGGCATTAAC AGGACAAAAT GGTGACCACA GTGAATATGT TCCAATGTCT 
TCACCTGGAA AACTCCTGTA TGGTGGACCA GAAAATGTAA AAAGCACCAA CAGCGAAGTT 
TTGTCTAACG GCATCTCTAA GTCACCGGTG GCAAAAGGTT CAGATGAAGG ACTGGTGCAG 
AACAGGGCTA CACGGCCAAC AAGACTCCCC CTCGGTACAA GAGGGAGTAA TACTATCCCA 
AGAATGTATG ATCGCACAGT TCCACCTGAG CCGGCAAGTC CTGGTGAATA CATCAATATT 
GATTTTAATG AGAAGGCAAG TAATACACCG TATTCCTTAT CTGCAGAAGG ATCGCCATCA 
TCTCTGGGCT CAAGTAGTGA CCACAGGCAG TCCCCACTCT CAGATTATAT GAGCGTAGAC 
TTGGATGTAC AGTCACCAAA AGTAGCGAAA GAACTATCTA ACTCTTTAAC AGATATTTCA 
ATTTATGCAA GTTCCAGTAT TCCTAGAAAC CAACAAAATC CTGACTATGC TAGGCTCTCC 
TTTGGTACTG CATGTGTTAG CGCTGCGAAT AACAGGACTG ATGATTACAC AGAGATGACA 
TTCAACATGG CAGCAACGCC ACCTCGACCC TTTGCTGCCG AATCCGATGA TGGTGTAAAG 
ATGGATAGTC CTTCTTCCAT AGTTAACAGA CTGTGCATTG TTGATCGATA TGCTGGTAGC 
AGTAGTTTTT CTGTTCCTAG TGCTGAGCCT CCAATGGGAC CGAAAGTTAT CCGAGCTGAT 
CCTCAAGGCA GGAGGAGACA CAGTTCTGAA ACCTTCTCTT CTGCTGGGAC TGTGACGACA 
TCCTCCTCTT TCTTTACTGA TAGTAGCAAA AGACACAGCT CTGCCTCATT TGATAATGTT 
TGGTTAAAAC CAGATGAAAG CATTTCTGAT GGTCAGGAAA GCAAAATGTC CAGGGATACC 
TCAACTGGAT TTCAAAATGG CTTAAACTAC ATAGCTCTGA ATTTATGCGA TGATCCAATA 
AGCTGTGAGG CAAGTACTGG GACACCAACT TGCCATCTCC AAAATGGTAC TTCAAGTTTG 
GACAGTGGAG CGTATGTAAG CATAGACTTC ACCAGGTCTG ATGGTCTGAA GTGTAACGCT 
GCACGAAAAG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003641132.3
CDS	245..3739
Translation	MASGMNGPGGGGGAGAAVRGGEEEPGARHAGGGAVPQQREPGAAGEGEPAAGDGGRCPSPQPHHLLLLLRRSPSASLCPPAEGPAGRAALGRGGQPPPAGRAAPPASSSGGDDVRKCGYLRKQKHGHKRYFVLRADSPLAPARLEYYDSEKKFKSSLRAGGGGAAPVCCPPPKRVIPLYQCFTVSRRADAKHKHIIALYTKDEYFAMLAENEAEQEAWYEAISELMSQTKRGCLEQEDQAEQQVDEDDEHYGTSLRPGTVFKEVWQVNVKPKGLGQTKNLTGVYRLCLSSKAIHLVKLNSEVPSVHLQLMNIRRCGHSENFFFIEVGRSASIGPGELWMQVDDSVVAQNMHETFLETMKALKAFAEFRPRSKSQSSGGGGGTNPISFITTRRHLGNLPPSQTGLQRRSRTESVVGGTPPTTKSSNSYRFRTSSEGEGTMTRPFRSVTGSLIHLNTARMNLGRQEGSGRYVRAAFSSSYHTRSASLPVSHFPSTTSPISVSSSSGHGSASDMLTRPSSSSVCGSPSDGGFISSDEYGSSPGDFRYFRVRSNTPDSLGNTPPIREENCLSEYMSMSKQQADDNSRDDYMEAEKCFRKRTYSLTKPTSVAVQQKTTQTTALLDEDSVGNHGRLLYSETSKLKGNHELEYSDSNLDSICNQSRSKARDDGYMPMMPGVASSLSSNSDYLPMTPKSMSVPKQINNSWSPSQVDSRGYMMMFPKASSSPVRSPLTGFISKGSNEKIVNNEYMDMSPGNSAPKHPGDSNYIHTSSASKGFSSYFSLPRSFKALTGQNGDHSEYVPMSSPGKLLYGGPENVKSTNSEVLSNGISKSPVAKGSDEGLVQNRATRPTRLPLGTRGSNTIPRMYDRTVPPEPASPGEYINIDFNEKASNTPYSLSAEGSPSSLGSSSDHRQSPLSDYMSVDLDVQSPKVAKELSNSLTDISIYASSSIPRNQQNPDYARLSFGTACVSAANNRTDDYTEMTFNMAATPPRPFAAESDDGVKMDSPSSIVNRLCIVDRYAGSSSFSVPSAEPPMGPKVIRADPQGRRRHSSETFSSAGTVTTSSSFFTDSSKRHSSASFDNVWLKPDESISDGQESKMSRDTSTGFQNGLNYIALNLCDDPISCEASTGTPTCHLQNGTSSLDSGAYVSIDFTRSDGLKCNAARKD

Target ORF information:

RefSeq Version	XM_003641084.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus insulin receptor substrate 4 (IRS4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003641084.3

ORF Insert Sequence:

ATGGCGAGCG GCATGAACGG CCCGGGCGGC GGCGGCGGCG CCGGCGCGGC GGTCAGGGGC 
GGCGAGGAGG AGCCGGGCGC CCGGCACGCA GGAGGCGGAG CCGTGCCTCA GCAGCGGGAA 
CCCGGTGCGG CGGGCGAGGG GGAGCCGGCG GCGGGGGACG GTGGGCGCTG CCCGTCCCCG 
CAGCCCCACC ACCTGCTGCT GCTGCTGCGG CGCTCTCCCA GCGCTTCGCT CTGTCCGCCC 
GCGGAGGGCC CCGCGGGCCG CGCCGCCCTG GGCCGCGGCG GGCAGCCCCC CCCTGCGGGC 
CGCGCCGCAC CCCCGGCCTC GTCCTCCGGC GGCGACGACG TGAGGAAGTG CGGCTACCTG 
CGGAAGCAGA AGCACGGGCA CAAGCGGTAC TTCGTGCTGC GGGCCGATAG CCCGCTGGCC 
CCTGCCCGCC TGGAGTACTA CGACAGCGAG AAGAAGTTCA AGAGCAGCCT GCGGGCGGGG 
GGCGGCGGGG CGGCCCCTGT CTGCTGCCCA CCGCCCAAGC GGGTCATCCC GCTCTACCAG 
TGCTTCACCG TCAGCCGGCG CGCCGATGCC AAGCACAAGC ACATCATTGC CTTGTACACC 
AAGGATGAGT ACTTTGCTAT GCTGGCGGAG AATGAGGCCG AGCAGGAGGC CTGGTACGAA 
GCCATCAGCG AGCTGATGAG CCAGACCAAG AGGGGCTGCC TGGAACAGGA GGACCAGGCG 
GAGCAGCAGG TGGATGAGGA CGACGAGCAC TACGGGACCT CCCTGAGGCC TGGCACTGTC 
TTCAAGGAGG TGTGGCAGGT TAACGTGAAG CCCAAAGGGC TGGGCCAGAC GAAAAACCTC 
ACTGGAGTGT ATCGGCTGTG CCTCTCCAGC AAGGCTATCC ACCTTGTTAA GCTTAACTCG 
GAGGTGCCCT CTGTCCACCT ACAGCTGATG AATATTCGCC GCTGTGGGCA CTCAGAGAAC 
TTCTTCTTCA TCGAAGTGGG CAGATCAGCC TCCATCGGGC CTGGAGAGCT CTGGATGCAA 
GTGGATGATT CTGTGGTGGC CCAGAATATG CACGAGACTT TTTTGGAGAC CATGAAAGCG 
CTCAAAGCCT TTGCAGAGTT TAGGCCCCGA AGCAAAAGCC AGTCTTCTGG TGGTGGTGGT 
GGTACCAATC CCATCTCCTT CATCACCACT AGGAGGCACC TGGGCAACCT GCCCCCCAGC 
CAGACGGGCT TACAGAGAAG ATCGAGAACT GAGAGTGTAG TTGGAGGGAC GCCTCCTACC 
ACCAAGAGCA GCAACTCCTA CCGCTTCAGG ACATCCAGTG AGGGAGAAGG AACCATGACT 
AGACCTTTCA GGTCAGTGAC AGGGAGCCTG ATCCACCTCA ACACTGCAAG GATGAATTTG 
GGCCGGCAAG AAGGGAGTGG AAGGTACGTT AGAGCTGCAT TCAGCTCATC ATATCACACT 
AGGTCTGCTT CGCTGCCTGT GTCTCATTTT CCCTCCACTA CGAGTCCTAT CAGCGTTTCT 
TCCAGCAGTG GCCATGGCTC TGCTTCAGAC ATGTTGACCA GGCCTTCTAG CTCGTCTGTT 
TGTGGCTCTC CAAGTGATGG GGGATTTATC TCTTCTGATG AGTATGGCTC CAGCCCTGGA 
GATTTCAGGT ACTTTAGGGT CCGGAGTAAT ACACCGGATT CCTTGGGAAA CACACCGCCT 
ATCAGAGAGG AGAATTGTCT GAGTGAGTAC ATGTCCATGA GTAAGCAGCA AGCAGACGAT 
AACTCAAGAG ATGATTATAT GGAGGCTGAA AAGTGTTTCA GAAAAAGAAC TTACTCTCTA 
ACTAAACCAA CTTCGGTAGC AGTGCAGCAG AAAACAACAC AAACTACAGC TTTGTTAGAT 
GAAGATTCCG TAGGAAATCA TGGACGATTA CTTTACTCTG AAACATCAAA ATTGAAAGGT 
AACCATGAAT TGGAGTACAG TGACAGTAAT CTTGATTCTA TATGCAACCA AAGCAGGAGT 
AAAGCCAGGG ATGATGGATA CATGCCAATG ATGCCAGGCG TTGCGTCCTC TCTGTCAAGT 
AACAGCGATT ATTTGCCAAT GACTCCTAAA AGTATGTCTG TTCCGAAGCA GATTAACAAT 
TCATGGTCAC CATCTCAGGT CGACTCAAGA GGATATATGA TGATGTTTCC AAAGGCAAGC 
TCTTCACCTG TACGGAGTCC TTTAACTGGA TTTATTTCTA AAGGTAGTAA CGAGAAGATT 
GTCAACAATG AATACATGGA TATGTCACCT GGTAATTCAG CTCCAAAGCA TCCCGGTGAT 
TCAAATTATA TACATACTAG TTCTGCCTCC AAAGGTTTCA GTTCATATTT TTCTTTGCCT 
CGAAGCTTTA AGGCATTAAC AGGACAAAAT GGTGACCACA GTGAATATGT TCCAATGTCT 
TCACCTGGAA AACTCCTGTA TGGTGGACCA GAAAATGTAA AAAGCACCAA CAGCGAAGTT 
TTGTCTAACG GCATCTCTAA GTCACCGGTG GCAAAAGGTT CAGATGAAGG ACTGGTGCAG 
AACAGGGCTA CACGGCCAAC AAGACTCCCC CTCGGTACAA GAGGGAGTAA TACTATCCCA 
AGAATGTATG ATCGCACAGT TCCACCTGAG CCGGCAAGTC CTGGTGAATA CATCAATATT 
GATTTTAATG AGAAGGCAAG TAATACACCG TATTCCTTAT CTGCAGAAGG ATCGCCATCA 
TCTCTGGGCT CAAGTAGTGA CCACAGGCAG TCCCCACTCT CAGATTATAT GAGCGTAGAC 
TTGGATGTAC AGTCACCAAA AGTAGCGAAA GAACTATCTA ACTCTTTAAC AGATATTTCA 
ATTTATGCAA GTTCCAGTAT TCCTAGAAAC CAACAAAATC CTGACTATGC TAGGCTCTCC 
TTTGGTACTG CATGTGTTAG CGCTGCGAAT AACAGGACTG ATGATTACAC AGAGATGACA 
TTCAACATGG CAGCAACGCC ACCTCGACCC TTTGCTGCCG AATCCGATGA TGGTGTAAAG 
ATGGATAGTC CTTCTTCCAT AGTTAACAGA CTGTGCATTG TTGATCGATA TGCTGGTAGC 
AGTAGTTTTT CTGTTCCTAG TGCTGAGCCT CCAATGGGAC CGAAAGTTAT CCGAGCTGAT 
CCTCAAGGCA GGAGGAGACA CAGTTCTGAA ACCTTCTCTT CTGCTGGGAC TGTGACGACA 
TCCTCCTCTT TCTTTACTGA TAGTAGCAAA AGACACAGCT CTGCCTCATT TGATAATGTT 
TGGTTAAAAC CAGATGAAAG CATTTCTGAT GGTCAGGAAA GCAAAATGTC CAGGGATACC 
TCAACTGGAT TTCAAAATGG CTTAAACTAC ATAGCTCTGA ATTTATGCGA TGATCCAATA 
AGCTGTGAGG CAAGTACTGG GACACCAACT TGCCATCTCC AAAATGGTAC TTCAAGTTTG 
GACAGTGGAG CGTATGTAAG CATAGACTTC ACCAGGTCTG ATGGTCTGAA GTGTAACGCT 
GCACGAAAAG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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IRS4 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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