f8 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	f8
Entrez Gene ID	100302404
Full Name	coagulation factor VIII, procoagulant component
General protein information	Preferred Names coagulation factor VIII, procoagulant component Names coagulation factor VIII
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009307649.2	XP_009305924.1	coagulation factor VIII
XM_009307649.3	XP_009305924.1	coagulation factor VIII

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	F8	XP_420193.4
Danio rerio (zebrafish)	f8	XP_002664307.3
Xenopus tropicalis (tropical clawed frog)	LOC100493411	XP_002941185.2

Gene Ontology

The following f8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the f8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa19220	XM_009307649.3 Latest version!	Danio rerio coagulation factor VIII, procoagulant component (f8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
ODa19220	XM_009307649.2	Danio rerio coagulation factor VIII, procoagulant component (f8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

f8 ( XM_009307649.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009305924.1 Danio rerio coagulation factor VIII, procoagulant component (f8), mRNA.

Price & Availability↑

CloneID	ODa19220
Clone ID Related Accession (Same CDS sequence)	XM_009307649.2 , XM_009307649.3
Accession Version	XM_009307649.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	coagulation factor VIII
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009307649.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACTC TTCTGTTCCT CCTCCTCCTC TCCGCGTGTC AGGAGTATTC GTGTGTGTCG 
GCTAAAACTT TAGAGTTCTT CATCGCAGCG GTCGAGACTA AATGGGAGTA TGTGTTTACA 
GACACAGCAG ATCCAGCCGC AAACCAGAGG AAGTTTGCAT CTGGTGTCCA GAAGTACACC 
AAGGCTGTAT ATACGGAGTA CACCGATTCC ACTTTCACTA CACCCAAGTC AAAACCTCCA 
TGGGCTGGCA TTCAAGGCCC GACTATCAGA ACAGTGGTGA ACGACAGGGT TGTGGTGCAC 
TTCAAAAACT TTGCATCCCA ATCCTTCAGT ATATCTCCCA TCGGCATTCC CTACTGGAAA 
CAATCAGAAG GCGCGGGATA TGAGGACTCC AGCTCTTTTC AGGAGCGTGC GGATGACTCG 
GTGCCCCCTG GTGGCTACTA TAGATACGTG TGGGACATTT CTCCAAACTC TGGGCCGACC 
ACATCTGACC CAGACTGCCT GACCTACATC TACTCATCAC AGGTGGACAT CATTCGAGAC 
TTCAACAGCG GACTCATCGG CGCACTGCTC ATCTGCAAGA CAGACGCTCT AACCGAGGAC 
AGACAGAGGA GAGTGCCAGA GTTCATTCTG CTTCTCTCGG TCTTCGATGA GAGCAAAAGC 
TGGTATCAGG AAGTGGGTCG AGCAGAAAAA CTGAAGAAAA CCTCCAGCAC AAACCAGTAC 
CACACCATCA ACGGCTACAT CAACTCCACA TTACCGGGTC TGAGTGTGTG TGAGAAGAGT 
CCGCAGGTCT CCTGGCACCT CATAGGTCTG GGCTCCAGTC CTGAGATCCA CAGCATCCAG 
TTTGAAGGAC ACACGCTGAA GGTGTTCGAC CACAAGCGGG TGACGGTGGA GATGACACCC 
ATGACCTTCA CCACAGCCCT CATGAAGCCC TCGGCCACGG GGAGGTTTCT GATCTCCTGC 
AGGATCCACT CGCATCAGAC AGCTGGTATG AATGCGTTCT TCACTGTTGA AAACTGTCCT 
GAACCCGCAC CGCTTCCCCT CCCGGACAAG AGAAACGTCA AGCACACTAA TGACGATGAG 
GAGGAGGATG ATGAAGACAA CATGTTCTCT GTGGTGTTCA AACAAGGAGG TCCAATATCT 
GTGCTTCGCT CCAGCGCGAA GGGCCGACCC AAAGTGTGGC TCCATTACAT CTCTGCAGAG 
GAGACGGACT GGGATTATAC CGCTCAGAGC GGAGATCGGC CTGCATCTTC AGCCGGGTCC 
GAGTGGTTCA GAAAAGGCCC TCAGCGTCTG GGTGGTGTTT ATAAGAAAGT GGCGTTCGTG 
GAGTACACAG ACAAGACATT CAGCGTCAAG AAAACCACCG GGAGAACACT GATCGGTCCC 
GAGCTCCGAG GGGAAGTGGG AGACAAGTTT CAGATTGTGT TTAAAAATAT GGCCAGTCGT 
CCATTCAACA TTTATCCAAA CGGCCTGACC AGCGTTCAGC CTCTGAAGAC AACAAACAAA 
GACAAACAGG TAGATCTGCG TTCTCTGGCC GTTCCCCCAG GTGAGATTAT GACATACCTG 
TGGAAGCTGA CGGCTGAAGA TGGACCCACA GATGCCGACC CGCGATGTCT GACGCGGATT 
TACCAGAGTA CGCTGGATCC GGCCCGGGAC GTGGCGTCTG GGCTGGTGGG ACCCCTGATC 
ATCTGCAAAT CCCAGTCTCT GGATAAAAGA GGCAGAGTGG TGACGTCAGA TAAAGAGAAG 
AAGCTGTTGT TTACGGTGTT TGATGAAAAC AGAAGCTGGT ACTTCGAGGA AAACATCAAG 
AGAAACAGTG AAGATCCAGC TAAGATCAAC CGCAATGATC TGGATTTCTA CAATTCTAAC 
ATCATGCACA CTGTGAACGG TTTCATGTTC AACCACCTGC AGTACAAGAT GTGTGTTGGC 
GATGTGATCC TCTGGCATGT GGCGAGCATC GGCATGCAAA ACAACTTCCT GTCAGTTTAT 
TTCACCGGAA ATACATTTGA GCGGGAAAAA GCGTACGGCA CCGTGCTCAC GCTTTTCCCA 
ATGACTGGAG ACACAGTCAC CACTGAAGTG GAGATGATGG GAGAATGGGA GGTCAGTGCA 
TTTGACCCGT CTCTAAAAGA GCGAGGTATG AGCGTCCGCT ATACCGTGGT AAACTGCGAA 
CGTGTGCTCC CCAAAGTAGA CTACGACGAC TACGATGAAG AATTCATGGA GATCATCGAA 
AAAAACTTCC CCAATCCACG AGGCACCATG GGAAAGAACC GCACTATCGC CATCCGCGTC 
TGCAGGAACA GAACTGTGAC GGATTCTGGG AGCAACACGA GACACAATGA GACGCTGAAC 
AGAAGCCGCG TGTGCGAGAT AAGATATGTT CCCGTAAAAG AAGAGAACGA TCTGGAAGCG 
TTATCGCAGG GCGGTATTCC TCAGGATATT CTGGATCAAC TGGACGATGA GATTGGAGGA 
AACTCGGTGG AAAGACAGAA AAGATCCCAC CAGGAATCCC ACACAGTCAC GTCCAGCTAC 
ACTGAATTAC CTCAAAACCA ACACAATGAA ACACAAGCAG GTGCTGACAC TACAAATGAG 
CTGCTCAGAA AATTCCTCTT CAAACATACA AACCTAAACA TCTCTAGAAG AACGCAGGAT 
GAGATGGAGG AAAGCTTGAA TCCGCTTAGC TTTAATACGA TCCGAAATAT CTCAATGGTG 
GAGATTGAAA AAGCAAGGAG TCGGAGAGAA CTGTGGAGCG TTGGGAAAAC TAACCAGAAA 
GATGTTAAAG CCATGAGTGA TAATGAAGTT TTAGAAGACT CTGGAAGAAA GCGGGATAAG 
ATGGAGGAAA GCTTGAACCC GCTGAGCTAT AATTATAAGG ACCTGAAGAA GTATTCGGAG 
ATGGGTTTGG AGCCGGCGTC TAACTCATCG GATGACAAGA TGTACGGGAA GAAGTCGGTG 
ACGAGCGATT ATGATGATTA CAGTGATGAG GGAAGCGTTG TCGGGTTAGA TCATTTTGTT 
GAGGATAATG TCGGCGTTCG GTCGACAGAA TCAAAGTTTA GGAGTTACTA CATCGCGGCT 
GAGGAAGTCA TGTGGGATTA TGGGATTGAT AAACCGGCTC AGCTCATCAA ACCGAGGGAG 
ATGCGGAGGG GACTGAGAAA ATACTTCCCA ACCTATAAAA AAGTGGTGTT TAGAGCGTAT 
CAGGACCGAG ATTTCAGGCA TCCAATCAAA CGTGGAGAAC TGGACGAGCA CCTGGGCATT 
ATGGGTCCTG TAGTGAAAGC AGAGATCAAT GATGTTCTCA CAGTGGTTTT TAAGAACCTG 
GCCTCCAGAC CGTACTCATT CCACTTGCAT GGAGTGTACG ATAAGACGCC GGGGAGTTTC 
AGCACTGGAA TGGGTCCAGA AAACCCCAAG CTGGAGGAAG AAGCAGGAGA TCCAGTTCCT 
CCAGGAGAGG AGAGAGTTTA TAACTGGAGA ATCTCCAGAC GCCAAGGACC CTCCGCCTCA 
GAGTTCGACT GCAAGGCTGG AGCTTATTAC TCCAACGTCA ACATGGAGAA GGACATAAAC 
TCTGGTCTGA TCGGCCCCAT GCTGATTTGT CGTCCTGGAA CGCTCAACCC ACGAGTCCTG 
CTTCAGCCTG ACGTCACAGA TTTCTTCCTT CTCTTCACCA CATTTGACGA AAGAAAGAGC 
TGGTACCTCG ACTACAACAT CAGGAAGTTC TGCATCCCGC CATGCCAGGC CAGAGTAGAC 
GATCCCTGGT TCCAGACTAG CAACAAGTTT GCAGCTATTA ACGGTTACGT GTCTGAGACG 
CTGCCTGGTC TGAGGGTTGA GCAGTACAGG ACGGCCCGCT GGCATCTGCT CAATCTGGGA 
ACTCAAGGCG AGTTTCACGC TGTACACTTC CACGGACTTC CATTCAGAGT GGGAAAGGAT 
CAGGAACACC GCATGGGAAT TTTCAATCTA TATCCTGGTG TTTTTGGGAC GGTGGAGATG 
CGGCCTGCCA TCGCAGGCAC CTGGTTAATA GAGTGCACGA TAGGGGAACA TCAGCTGGCA 
GGAATGAGGG CAAAACTGCT GGTCTATAAC CCACAATGCA ATCAGCCCAT AGGAATGCAA 
TCAAGATGGA TCACCAACAA ACAAATAACA GCGTCTGATT ACTACGGTGA CTGGAAGCCT 
GAATTAGCCA GACTTGAGTT AACTGGATCC ATTAATGCCT GGATGGGCAG CAGCCCAAAC 
TCCTGGCTGC AGGTGGATCT GTTAAGACCC ATGATCCTGC ATGGTATTCA GACTCAGGGT 
GCGAAGACTT CGATGGGTTT GAGCGAGTTC TTCACTATTC TCTACAGCAT CAGTTACAGC 
AACGACCAGG AGAACTGGAG CGTTTACAGA GGAAACAGCA CCAAACCATA TTACACCTTC 
AATGGAAACA TGGACGGTTC CAGAATAAAG GAGAACTTAT TTTCTCCACC CATCGTGGGA 
CGCTACATCC GCATTCACCC TCTGACCTTC CAGAAGCAGC CCACGCTGAG GATTGAACTG 
CTGGGGTGTG ACCTCAACAG CTGTTCTCTG CCACTGGGAA TGGAGCGCAG GTCGATTCCC 
AACAGCAGCA TCAGCGCCTC CTCATTCCTC AATAAGTGGC TGCTGTCCTG GAGCCCCGAC 
CTCGCCAGAA TACACCAGGA GGGACGAGCA AACGCTTGGA GACCAAAGTC TAATAACCCT 
CACGAGTGGC TCCAGGTGGA CTTTGCGTCA GTGAAGCGAG TGACCGGTTT GATCACACAG 
GGGGCTCGGT CCGTCCTGAA GCCCATGATG GTGACAGAAT TCACTGTTTC TGTCAGCAAT 
GATGGACACA GCTGGAGCAG TGTGACGGAG CAGGGCACCG ACAGCGAGCA GATCTTTGAG 
GGCAACAGTG ATAATAATGA GGAGACACTG AACATCTTTG ATCCTCCTCT CTTCACTCGT 
TTCATCCGCG TTCATCCTAA AGGCTGGGTG AACGACATCG CTCTGCGTCT GGAGTTTCTG 
GGCTGCGACA CACAACCAAC ACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009305924.1
CDS	12..5018
Translation	MKTLLFLLLLSACQEYSCVSAKTLEFFIAAVETKWEYVFTDTADPAANQRKFASGVQKYTKAVYTEYTDSTFTTPKSKPPWAGIQGPTIRTVVNDRVVVHFKNFASQSFSISPIGIPYWKQSEGAGYEDSSSFQERADDSVPPGGYYRYVWDISPNSGPTTSDPDCLTYIYSSQVDIIRDFNSGLIGALLICKTDALTEDRQRRVPEFILLLSVFDESKSWYQEVGRAEKLKKTSSTNQYHTINGYINSTLPGLSVCEKSPQVSWHLIGLGSSPEIHSIQFEGHTLKVFDHKRVTVEMTPMTFTTALMKPSATGRFLISCRIHSHQTAGMNAFFTVENCPEPAPLPLPDKRNVKHTNDDEEEDDEDNMFSVVFKQGGPISVLRSSAKGRPKVWLHYISAEETDWDYTAQSGDRPASSAGSEWFRKGPQRLGGVYKKVAFVEYTDKTFSVKKTTGRTLIGPELRGEVGDKFQIVFKNMASRPFNIYPNGLTSVQPLKTTNKDKQVDLRSLAVPPGEIMTYLWKLTAEDGPTDADPRCLTRIYQSTLDPARDVASGLVGPLIICKSQSLDKRGRVVTSDKEKKLLFTVFDENRSWYFEENIKRNSEDPAKINRNDLDFYNSNIMHTVNGFMFNHLQYKMCVGDVILWHVASIGMQNNFLSVYFTGNTFEREKAYGTVLTLFPMTGDTVTTEVEMMGEWEVSAFDPSLKERGMSVRYTVVNCERVLPKVDYDDYDEEFMEIIEKNFPNPRGTMGKNRTIAIRVCRNRTVTDSGSNTRHNETLNRSRVCEIRYVPVKEENDLEALSQGGIPQDILDQLDDEIGGNSVERQKRSHQESHTVTSSYTELPQNQHNETQAGADTTNELLRKFLFKHTNLNISRRTQDEMEESLNPLSFNTIRNISMVEIEKARSRRELWSVGKTNQKDVKAMSDNEVLEDSGRKRDKMEESLNPLSYNYKDLKKYSEMGLEPASNSSDDKMYGKKSVTSDYDDYSDEGSVVGLDHFVEDNVGVRSTESKFRSYYIAAEEVMWDYGIDKPAQLIKPREMRRGLRKYFPTYKKVVFRAYQDRDFRHPIKRGELDEHLGIMGPVVKAEINDVLTVVFKNLASRPYSFHLHGVYDKTPGSFSTGMGPENPKLEEEAGDPVPPGEERVYNWRISRRQGPSASEFDCKAGAYYSNVNMEKDINSGLIGPMLICRPGTLNPRVLLQPDVTDFFLLFTTFDERKSWYLDYNIRKFCIPPCQARVDDPWFQTSNKFAAINGYVSETLPGLRVEQYRTARWHLLNLGTQGEFHAVHFHGLPFRVGKDQEHRMGIFNLYPGVFGTVEMRPAIAGTWLIECTIGEHQLAGMRAKLLVYNPQCNQPIGMQSRWITNKQITASDYYGDWKPELARLELTGSINAWMGSSPNSWLQVDLLRPMILHGIQTQGAKTSMGLSEFFTILYSISYSNDQENWSVYRGNSTKPYYTFNGNMDGSRIKENLFSPPIVGRYIRIHPLTFQKQPTLRIELLGCDLNSCSLPLGMERRSIPNSSISASSFLNKWLLSWSPDLARIHQEGRANAWRPKSNNPHEWLQVDFASVKRVTGLITQGARSVLKPMMVTEFTVSVSNDGHSWSSVTEQGTDSEQIFEGNSDNNEETLNIFDPPLFTRFIRVHPKGWVNDIALRLEFLGCDTQPTL

Target ORF information:

RefSeq Version	XM_009307649.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio coagulation factor VIII, procoagulant component (f8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009307649.2

ORF Insert Sequence:

ATGAAGACTC TTCTGTTCCT CCTCCTCCTC TCCGCGTGTC AGGAGTATTC GTGTGTGTCG 
GCTAAAACTT TAGAGTTCTT CATCGCAGCG GTCGAGACTA AATGGGAGTA TGTGTTTACA 
GACACAGCAG ATCCAGCCGC AAACCAGAGG AAGTTTGCAT CTGGTGTCCA GAAGTACACC 
AAGGCTGTAT ATACGGAGTA CACCGATTCC ACTTTCACTA CACCCAAGTC AAAACCTCCA 
TGGGCTGGCA TTCAAGGCCC GACTATCAGA ACAGTGGTGA ACGACAGGGT TGTGGTGCAC 
TTCAAAAACT TTGCATCCCA ATCCTTCAGT ATATCTCCCA TCGGCATTCC CTACTGGAAA 
CAATCAGAAG GCGCGGGATA TGAGGACTCC AGCTCTTTTC AGGAGCGTGC GGATGACTCG 
GTGCCCCCTG GTGGCTACTA TAGATACGTG TGGGACATTT CTCCAAACTC TGGGCCGACC 
ACATCTGACC CAGACTGCCT GACCTACATC TACTCATCAC AGGTGGACAT CATTCGAGAC 
TTCAACAGCG GACTCATCGG CGCACTGCTC ATCTGCAAGA CAGACGCTCT AACCGAGGAC 
AGACAGAGGA GAGTGCCAGA GTTCATTCTG CTTCTCTCGG TCTTCGATGA GAGCAAAAGC 
TGGTATCAGG AAGTGGGTCG AGCAGAAAAA CTGAAGAAAA CCTCCAGCAC AAACCAGTAC 
CACACCATCA ACGGCTACAT CAACTCCACA TTACCGGGTC TGAGTGTGTG TGAGAAGAGT 
CCGCAGGTCT CCTGGCACCT CATAGGTCTG GGCTCCAGTC CTGAGATCCA CAGCATCCAG 
TTTGAAGGAC ACACGCTGAA GGTGTTCGAC CACAAGCGGG TGACGGTGGA GATGACACCC 
ATGACCTTCA CCACAGCCCT CATGAAGCCC TCGGCCACGG GGAGGTTTCT GATCTCCTGC 
AGGATCCACT CGCATCAGAC AGCTGGTATG AATGCGTTCT TCACTGTTGA AAACTGTCCT 
GAACCCGCAC CGCTTCCCCT CCCGGACAAG AGAAACGTCA AGCACACTAA TGACGATGAG 
GAGGAGGATG ATGAAGACAA CATGTTCTCT GTGGTGTTCA AACAAGGAGG TCCAATATCT 
GTGCTTCGCT CCAGCGCGAA GGGCCGACCC AAAGTGTGGC TCCATTACAT CTCTGCAGAG 
GAGACGGACT GGGATTATAC CGCTCAGAGC GGAGATCGGC CTGCATCTTC AGCCGGGTCC 
GAGTGGTTCA GAAAAGGCCC TCAGCGTCTG GGTGGTGTTT ATAAGAAAGT GGCGTTCGTG 
GAGTACACAG ACAAGACATT CAGCGTCAAG AAAACCACCG GGAGAACACT GATCGGTCCC 
GAGCTCCGAG GGGAAGTGGG AGACAAGTTT CAGATTGTGT TTAAAAATAT GGCCAGTCGT 
CCATTCAACA TTTATCCAAA CGGCCTGACC AGCGTTCAGC CTCTGAAGAC AACAAACAAA 
GACAAACAGG TAGATCTGCG TTCTCTGGCC GTTCCCCCAG GTGAGATTAT GACATACCTG 
TGGAAGCTGA CGGCTGAAGA TGGACCCACA GATGCCGACC CGCGATGTCT GACGCGGATT 
TACCAGAGTA CGCTGGATCC GGCCCGGGAC GTGGCGTCTG GGCTGGTGGG ACCCCTGATC 
ATCTGCAAAT CCCAGTCTCT GGATAAAAGA GGCAGAGTGG TGACGTCAGA TAAAGAGAAG 
AAGCTGTTGT TTACGGTGTT TGATGAAAAC AGAAGCTGGT ACTTCGAGGA AAACATCAAG 
AGAAACAGTG AAGATCCAGC TAAGATCAAC CGCAATGATC TGGATTTCTA CAATTCTAAC 
ATCATGCACA CTGTGAACGG TTTCATGTTC AACCACCTGC AGTACAAGAT GTGTGTTGGC 
GATGTGATCC TCTGGCATGT GGCGAGCATC GGCATGCAAA ACAACTTCCT GTCAGTTTAT 
TTCACCGGAA ATACATTTGA GCGGGAAAAA GCGTACGGCA CCGTGCTCAC GCTTTTCCCA 
ATGACTGGAG ACACAGTCAC CACTGAAGTG GAGATGATGG GAGAATGGGA GGTCAGTGCA 
TTTGACCCGT CTCTAAAAGA GCGAGGTATG AGCGTCCGCT ATACCGTGGT AAACTGCGAA 
CGTGTGCTCC CCAAAGTAGA CTACGACGAC TACGATGAAG AATTCATGGA GATCATCGAA 
AAAAACTTCC CCAATCCACG AGGCACCATG GGAAAGAACC GCACTATCGC CATCCGCGTC 
TGCAGGAACA GAACTGTGAC GGATTCTGGG AGCAACACGA GACACAATGA GACGCTGAAC 
AGAAGCCGCG TGTGCGAGAT AAGATATGTT CCCGTAAAAG AAGAGAACGA TCTGGAAGCG 
TTATCGCAGG GCGGTATTCC TCAGGATATT CTGGATCAAC TGGACGATGA GATTGGAGGA 
AACTCGGTGG AAAGACAGAA AAGATCCCAC CAGGAATCCC ACACAGTCAC GTCCAGCTAC 
ACTGAATTAC CTCAAAACCA ACACAATGAA ACACAAGCAG GTGCTGACAC TACAAATGAG 
CTGCTCAGAA AATTCCTCTT CAAACATACA AACCTAAACA TCTCTAGAAG AACGCAGGAT 
GAGATGGAGG AAAGCTTGAA TCCGCTTAGC TTTAATACGA TCCGAAATAT CTCAATGGTG 
GAGATTGAAA AAGCAAGGAG TCGGAGAGAA CTGTGGAGCG TTGGGAAAAC TAACCAGAAA 
GATGTTAAAG CCATGAGTGA TAATGAAGTT TTAGAAGACT CTGGAAGAAA GCGGGATAAG 
ATGGAGGAAA GCTTGAACCC GCTGAGCTAT AATTATAAGG ACCTGAAGAA GTATTCGGAG 
ATGGGTTTGG AGCCGGCGTC TAACTCATCG GATGACAAGA TGTACGGGAA GAAGTCGGTG 
ACGAGCGATT ATGATGATTA CAGTGATGAG GGAAGCGTTG TCGGGTTAGA TCATTTTGTT 
GAGGATAATG TCGGCGTTCG GTCGACAGAA TCAAAGTTTA GGAGTTACTA CATCGCGGCT 
GAGGAAGTCA TGTGGGATTA TGGGATTGAT AAACCGGCTC AGCTCATCAA ACCGAGGGAG 
ATGCGGAGGG GACTGAGAAA ATACTTCCCA ACCTATAAAA AAGTGGTGTT TAGAGCGTAT 
CAGGACCGAG ATTTCAGGCA TCCAATCAAA CGTGGAGAAC TGGACGAGCA CCTGGGCATT 
ATGGGTCCTG TAGTGAAAGC AGAGATCAAT GATGTTCTCA CAGTGGTTTT TAAGAACCTG 
GCCTCCAGAC CGTACTCATT CCACTTGCAT GGAGTGTACG ATAAGACGCC GGGGAGTTTC 
AGCACTGGAA TGGGTCCAGA AAACCCCAAG CTGGAGGAAG AAGCAGGAGA TCCAGTTCCT 
CCAGGAGAGG AGAGAGTTTA TAACTGGAGA ATCTCCAGAC GCCAAGGACC CTCCGCCTCA 
GAGTTCGACT GCAAGGCTGG AGCTTATTAC TCCAACGTCA ACATGGAGAA GGACATAAAC 
TCTGGTCTGA TCGGCCCCAT GCTGATTTGT CGTCCTGGAA CGCTCAACCC ACGAGTCCTG 
CTTCAGCCTG ACGTCACAGA TTTCTTCCTT CTCTTCACCA CATTTGACGA AAGAAAGAGC 
TGGTACCTCG ACTACAACAT CAGGAAGTTC TGCATCCCGC CATGCCAGGC CAGAGTAGAC 
GATCCCTGGT TCCAGACTAG CAACAAGTTT GCAGCTATTA ACGGTTACGT GTCTGAGACG 
CTGCCTGGTC TGAGGGTTGA GCAGTACAGG ACGGCCCGCT GGCATCTGCT CAATCTGGGA 
ACTCAAGGCG AGTTTCACGC TGTACACTTC CACGGACTTC CATTCAGAGT GGGAAAGGAT 
CAGGAACACC GCATGGGAAT TTTCAATCTA TATCCTGGTG TTTTTGGGAC GGTGGAGATG 
CGGCCTGCCA TCGCAGGCAC CTGGTTAATA GAGTGCACGA TAGGGGAACA TCAGCTGGCA 
GGAATGAGGG CAAAACTGCT GGTCTATAAC CCACAATGCA ATCAGCCCAT AGGAATGCAA 
TCAAGATGGA TCACCAACAA ACAAATAACA GCGTCTGATT ACTACGGTGA CTGGAAGCCT 
GAATTAGCCA GACTTGAGTT AACTGGATCC ATTAATGCCT GGATGGGCAG CAGCCCAAAC 
TCCTGGCTGC AGGTGGATCT GTTAAGACCC ATGATCCTGC ATGGTATTCA GACTCAGGGT 
GCGAAGACTT CGATGGGTTT GAGCGAGTTC TTCACTATTC TCTACAGCAT CAGTTACAGC 
AACGACCAGG AGAACTGGAG CGTTTACAGA GGAAACAGCA CCAAACCATA TTACACCTTC 
AATGGAAACA TGGACGGTTC CAGAATAAAG GAGAACTTAT TTTCTCCACC CATCGTGGGA 
CGCTACATCC GCATTCACCC TCTGACCTTC CAGAAGCAGC CCACGCTGAG GATTGAACTG 
CTGGGGTGTG ACCTCAACAG CTGTTCTCTG CCACTGGGAA TGGAGCGCAG GTCGATTCCC 
AACAGCAGCA TCAGCGCCTC CTCATTCCTC AATAAGTGGC TGCTGTCCTG GAGCCCCGAC 
CTCGCCAGAA TACACCAGGA GGGACGAGCA AACGCTTGGA GACCAAAGTC TAATAACCCT 
CACGAGTGGC TCCAGGTGGA CTTTGCGTCA GTGAAGCGAG TGACCGGTTT GATCACACAG 
GGGGCTCGGT CCGTCCTGAA GCCCATGATG GTGACAGAAT TCACTGTTTC TGTCAGCAAT 
GATGGACACA GCTGGAGCAG TGTGACGGAG CAGGGCACCG ACAGCGAGCA GATCTTTGAG 
GGCAACAGTG ATAATAATGA GGAGACACTG AACATCTTTG ATCCTCCTCT CTTCACTCGT 
TTCATCCGCG TTCATCCTAA AGGCTGGGTG AACGACATCG CTCTGCGTCT GGAGTTTCTG 
GGCTGCGACA CACAACCAAC ACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

f8 ( XM_009307649.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009305924.1 Danio rerio coagulation factor VIII, procoagulant component (f8), mRNA.

Price & Availability↑

CloneID	ODa19220
Clone ID Related Accession (Same CDS sequence)	XM_009307649.2 , XM_009307649.3
Accession Version	XM_009307649.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	coagulation factor VIII
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009307649.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACTC TTCTGTTCCT CCTCCTCCTC TCCGCGTGTC AGGAGTATTC GTGTGTGTCG 
GCTAAAACTT TAGAGTTCTT CATCGCAGCG GTCGAGACTA AATGGGAGTA TGTGTTTACA 
GACACAGCAG ATCCAGCCGC AAACCAGAGG AAGTTTGCAT CTGGTGTCCA GAAGTACACC 
AAGGCTGTAT ATACGGAGTA CACCGATTCC ACTTTCACTA CACCCAAGTC AAAACCTCCA 
TGGGCTGGCA TTCAAGGCCC GACTATCAGA ACAGTGGTGA ACGACAGGGT TGTGGTGCAC 
TTCAAAAACT TTGCATCCCA ATCCTTCAGT ATATCTCCCA TCGGCATTCC CTACTGGAAA 
CAATCAGAAG GCGCGGGATA TGAGGACTCC AGCTCTTTTC AGGAGCGTGC GGATGACTCG 
GTGCCCCCTG GTGGCTACTA TAGATACGTG TGGGACATTT CTCCAAACTC TGGGCCGACC 
ACATCTGACC CAGACTGCCT GACCTACATC TACTCATCAC AGGTGGACAT CATTCGAGAC 
TTCAACAGCG GACTCATCGG CGCACTGCTC ATCTGCAAGA CAGACGCTCT AACCGAGGAC 
AGACAGAGGA GAGTGCCAGA GTTCATTCTG CTTCTCTCGG TCTTCGATGA GAGCAAAAGC 
TGGTATCAGG AAGTGGGTCG AGCAGAAAAA CTGAAGAAAA CCTCCAGCAC AAACCAGTAC 
CACACCATCA ACGGCTACAT CAACTCCACA TTACCGGGTC TGAGTGTGTG TGAGAAGAGT 
CCGCAGGTCT CCTGGCACCT CATAGGTCTG GGCTCCAGTC CTGAGATCCA CAGCATCCAG 
TTTGAAGGAC ACACGCTGAA GGTGTTCGAC CACAAGCGGG TGACGGTGGA GATGACACCC 
ATGACCTTCA CCACAGCCCT CATGAAGCCC TCGGCCACGG GGAGGTTTCT GATCTCCTGC 
AGGATCCACT CGCATCAGAC AGCTGGTATG AATGCGTTCT TCACTGTTGA AAACTGTCCT 
GAACCCGCAC CGCTTCCCCT CCCGGACAAG AGAAACGTCA AGCACACTAA TGACGATGAG 
GAGGAGGATG ATGAAGACAA CATGTTCTCT GTGGTGTTCA AACAAGGAGG TCCAATATCT 
GTGCTTCGCT CCAGCGCGAA GGGCCGACCC AAAGTGTGGC TCCATTACAT CTCTGCAGAG 
GAGACGGACT GGGATTATAC CGCTCAGAGC GGAGATCGGC CTGCATCTTC AGCCGGGTCC 
GAGTGGTTCA GAAAAGGCCC TCAGCGTCTG GGTGGTGTTT ATAAGAAAGT GGCGTTCGTG 
GAGTACACAG ACAAGACATT CAGCGTCAAG AAAACCACCG GGAGAACACT GATCGGTCCC 
GAGCTCCGAG GGGAAGTGGG AGACAAGTTT CAGATTGTGT TTAAAAATAT GGCCAGTCGT 
CCATTCAACA TTTATCCAAA CGGCCTGACC AGCGTTCAGC CTCTGAAGAC AACAAACAAA 
GACAAACAGG TAGATCTGCG TTCTCTGGCC GTTCCCCCAG GTGAGATTAT GACATACCTG 
TGGAAGCTGA CGGCTGAAGA TGGACCCACA GATGCCGACC CGCGATGTCT GACGCGGATT 
TACCAGAGTA CGCTGGATCC GGCCCGGGAC GTGGCGTCTG GGCTGGTGGG ACCCCTGATC 
ATCTGCAAAT CCCAGTCTCT GGATAAAAGA GGCAGAGTGG TGACGTCAGA TAAAGAGAAG 
AAGCTGTTGT TTACGGTGTT TGATGAAAAC AGAAGCTGGT ACTTCGAGGA AAACATCAAG 
AGAAACAGTG AAGATCCAGC TAAGATCAAC CGCAATGATC TGGATTTCTA CAATTCTAAC 
ATCATGCACA CTGTGAACGG TTTCATGTTC AACCACCTGC AGTACAAGAT GTGTGTTGGC 
GATGTGATCC TCTGGCATGT GGCGAGCATC GGCATGCAAA ACAACTTCCT GTCAGTTTAT 
TTCACCGGAA ATACATTTGA GCGGGAAAAA GCGTACGGCA CCGTGCTCAC GCTTTTCCCA 
ATGACTGGAG ACACAGTCAC CACTGAAGTG GAGATGATGG GAGAATGGGA GGTCAGTGCA 
TTTGACCCGT CTCTAAAAGA GCGAGGTATG AGCGTCCGCT ATACCGTGGT AAACTGCGAA 
CGTGTGCTCC CCAAAGTAGA CTACGACGAC TACGATGAAG AATTCATGGA GATCATCGAA 
AAAAACTTCC CCAATCCACG AGGCACCATG GGAAAGAACC GCACTATCGC CATCCGCGTC 
TGCAGGAACA GAACTGTGAC GGATTCTGGG AGCAACACGA GACACAATGA GACGCTGAAC 
AGAAGCCGCG TGTGCGAGAT AAGATATGTT CCCGTAAAAG AAGAGAACGA TCTGGAAGCG 
TTATCGCAGG GCGGTATTCC TCAGGATATT CTGGATCAAC TGGACGATGA GATTGGAGGA 
AACTCGGTGG AAAGACAGAA AAGATCCCAC CAGGAATCCC ACACAGTCAC GTCCAGCTAC 
ACTGAATTAC CTCAAAACCA ACACAATGAA ACACAAGCAG GTGCTGACAC TACAAATGAG 
CTGCTCAGAA AATTCCTCTT CAAACATACA AACCTAAACA TCTCTAGAAG AACGCAGGAT 
GAGATGGAGG AAAGCTTGAA TCCGCTTAGC TTTAATACGA TCCGAAATAT CTCAATGGTG 
GAGATTGAAA AAGCAAGGAG TCGGAGAGAA CTGTGGAGCG TTGGGAAAAC TAACCAGAAA 
GATGTTAAAG CCATGAGTGA TAATGAAGTT TTAGAAGACT CTGGAAGAAA GCGGGATAAG 
ATGGAGGAAA GCTTGAACCC GCTGAGCTAT AATTATAAGG ACCTGAAGAA GTATTCGGAG 
ATGGGTTTGG AGCCGGCGTC TAACTCATCG GATGACAAGA TGTACGGGAA GAAGTCGGTG 
ACGAGCGATT ATGATGATTA CAGTGATGAG GGAAGCGTTG TCGGGTTAGA TCATTTTGTT 
GAGGATAATG TCGGCGTTCG GTCGACAGAA TCAAAGTTTA GGAGTTACTA CATCGCGGCT 
GAGGAAGTCA TGTGGGATTA TGGGATTGAT AAACCGGCTC AGCTCATCAA ACCGAGGGAG 
ATGCGGAGGG GACTGAGAAA ATACTTCCCA ACCTATAAAA AAGTGGTGTT TAGAGCGTAT 
CAGGACCGAG ATTTCAGGCA TCCAATCAAA CGTGGAGAAC TGGACGAGCA CCTGGGCATT 
ATGGGTCCTG TAGTGAAAGC AGAGATCAAT GATGTTCTCA CAGTGGTTTT TAAGAACCTG 
GCCTCCAGAC CGTACTCATT CCACTTGCAT GGAGTGTACG ATAAGACGCC GGGGAGTTTC 
AGCACTGGAA TGGGTCCAGA AAACCCCAAG CTGGAGGAAG AAGCAGGAGA TCCAGTTCCT 
CCAGGAGAGG AGAGAGTTTA TAACTGGAGA ATCTCCAGAC GCCAAGGACC CTCCGCCTCA 
GAGTTCGACT GCAAGGCTGG AGCTTATTAC TCCAACGTCA ACATGGAGAA GGACATAAAC 
TCTGGTCTGA TCGGCCCCAT GCTGATTTGT CGTCCTGGAA CGCTCAACCC ACGAGTCCTG 
CTTCAGCCTG ACGTCACAGA TTTCTTCCTT CTCTTCACCA CATTTGACGA AAGAAAGAGC 
TGGTACCTCG ACTACAACAT CAGGAAGTTC TGCATCCCGC CATGCCAGGC CAGAGTAGAC 
GATCCCTGGT TCCAGACTAG CAACAAGTTT GCAGCTATTA ACGGTTACGT GTCTGAGACG 
CTGCCTGGTC TGAGGGTTGA GCAGTACAGG ACGGCCCGCT GGCATCTGCT CAATCTGGGA 
ACTCAAGGCG AGTTTCACGC TGTACACTTC CACGGACTTC CATTCAGAGT GGGAAAGGAT 
CAGGAACACC GCATGGGAAT TTTCAATCTA TATCCTGGTG TTTTTGGGAC GGTGGAGATG 
CGGCCTGCCA TCGCAGGCAC CTGGTTAATA GAGTGCACGA TAGGGGAACA TCAGCTGGCA 
GGAATGAGGG CAAAACTGCT GGTCTATAAC CCACAATGCA ATCAGCCCAT AGGAATGCAA 
TCAAGATGGA TCACCAACAA ACAAATAACA GCGTCTGATT ACTACGGTGA CTGGAAGCCT 
GAATTAGCCA GACTTGAGTT AACTGGATCC ATTAATGCCT GGATGGGCAG CAGCCCAAAC 
TCCTGGCTGC AGGTGGATCT GTTAAGACCC ATGATCCTGC ATGGTATTCA GACTCAGGGT 
GCGAAGACTT CGATGGGTTT GAGCGAGTTC TTCACTATTC TCTACAGCAT CAGTTACAGC 
AACGACCAGG AGAACTGGAG CGTTTACAGA GGAAACAGCA CCAAACCATA TTACACCTTC 
AATGGAAACA TGGACGGTTC CAGAATAAAG GAGAACTTAT TTTCTCCACC CATCGTGGGA 
CGCTACATCC GCATTCACCC TCTGACCTTC CAGAAGCAGC CCACGCTGAG GATTGAACTG 
CTGGGGTGTG ACCTCAACAG CTGTTCTCTG CCACTGGGAA TGGAGCGCAG GTCGATTCCC 
AACAGCAGCA TCAGCGCCTC CTCATTCCTC AATAAGTGGC TGCTGTCCTG GAGCCCCGAC 
CTCGCCAGAA TACACCAGGA GGGACGAGCA AACGCTTGGA GACCAAAGTC TAATAACCCT 
CACGAGTGGC TCCAGGTGGA CTTTGCGTCA GTGAAGCGAG TGACCGGTTT GATCACACAG 
GGGGCTCGGT CCGTCCTGAA GCCCATGATG GTGACAGAAT TCACTGTTTC TGTCAGCAAT 
GATGGACACA GCTGGAGCAG TGTGACGGAG CAGGGCACCG ACAGCGAGCA GATCTTTGAG 
GGCAACAGTG ATAATAATGA GGAGACACTG AACATCTTTG ATCCTCCTCT CTTCACTCGT 
TTCATCCGCG TTCATCCTAA AGGCTGGGTG AACGACATCG CTCTGCGTCT GGAGTTTCTG 
GGCTGCGACA CACAACCAAC ACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009305924.1
CDS	41..5047
Translation	MKTLLFLLLLSACQEYSCVSAKTLEFFIAAVETKWEYVFTDTADPAANQRKFASGVQKYTKAVYTEYTDSTFTTPKSKPPWAGIQGPTIRTVVNDRVVVHFKNFASQSFSISPIGIPYWKQSEGAGYEDSSSFQERADDSVPPGGYYRYVWDISPNSGPTTSDPDCLTYIYSSQVDIIRDFNSGLIGALLICKTDALTEDRQRRVPEFILLLSVFDESKSWYQEVGRAEKLKKTSSTNQYHTINGYINSTLPGLSVCEKSPQVSWHLIGLGSSPEIHSIQFEGHTLKVFDHKRVTVEMTPMTFTTALMKPSATGRFLISCRIHSHQTAGMNAFFTVENCPEPAPLPLPDKRNVKHTNDDEEEDDEDNMFSVVFKQGGPISVLRSSAKGRPKVWLHYISAEETDWDYTAQSGDRPASSAGSEWFRKGPQRLGGVYKKVAFVEYTDKTFSVKKTTGRTLIGPELRGEVGDKFQIVFKNMASRPFNIYPNGLTSVQPLKTTNKDKQVDLRSLAVPPGEIMTYLWKLTAEDGPTDADPRCLTRIYQSTLDPARDVASGLVGPLIICKSQSLDKRGRVVTSDKEKKLLFTVFDENRSWYFEENIKRNSEDPAKINRNDLDFYNSNIMHTVNGFMFNHLQYKMCVGDVILWHVASIGMQNNFLSVYFTGNTFEREKAYGTVLTLFPMTGDTVTTEVEMMGEWEVSAFDPSLKERGMSVRYTVVNCERVLPKVDYDDYDEEFMEIIEKNFPNPRGTMGKNRTIAIRVCRNRTVTDSGSNTRHNETLNRSRVCEIRYVPVKEENDLEALSQGGIPQDILDQLDDEIGGNSVERQKRSHQESHTVTSSYTELPQNQHNETQAGADTTNELLRKFLFKHTNLNISRRTQDEMEESLNPLSFNTIRNISMVEIEKARSRRELWSVGKTNQKDVKAMSDNEVLEDSGRKRDKMEESLNPLSYNYKDLKKYSEMGLEPASNSSDDKMYGKKSVTSDYDDYSDEGSVVGLDHFVEDNVGVRSTESKFRSYYIAAEEVMWDYGIDKPAQLIKPREMRRGLRKYFPTYKKVVFRAYQDRDFRHPIKRGELDEHLGIMGPVVKAEINDVLTVVFKNLASRPYSFHLHGVYDKTPGSFSTGMGPENPKLEEEAGDPVPPGEERVYNWRISRRQGPSASEFDCKAGAYYSNVNMEKDINSGLIGPMLICRPGTLNPRVLLQPDVTDFFLLFTTFDERKSWYLDYNIRKFCIPPCQARVDDPWFQTSNKFAAINGYVSETLPGLRVEQYRTARWHLLNLGTQGEFHAVHFHGLPFRVGKDQEHRMGIFNLYPGVFGTVEMRPAIAGTWLIECTIGEHQLAGMRAKLLVYNPQCNQPIGMQSRWITNKQITASDYYGDWKPELARLELTGSINAWMGSSPNSWLQVDLLRPMILHGIQTQGAKTSMGLSEFFTILYSISYSNDQENWSVYRGNSTKPYYTFNGNMDGSRIKENLFSPPIVGRYIRIHPLTFQKQPTLRIELLGCDLNSCSLPLGMERRSIPNSSISASSFLNKWLLSWSPDLARIHQEGRANAWRPKSNNPHEWLQVDFASVKRVTGLITQGARSVLKPMMVTEFTVSVSNDGHSWSSVTEQGTDSEQIFEGNSDNNEETLNIFDPPLFTRFIRVHPKGWVNDIALRLEFLGCDTQPTL

Target ORF information:

RefSeq Version	XM_009307649.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio coagulation factor VIII, procoagulant component (f8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009307649.3

ORF Insert Sequence:

ATGAAGACTC TTCTGTTCCT CCTCCTCCTC TCCGCGTGTC AGGAGTATTC GTGTGTGTCG 
GCTAAAACTT TAGAGTTCTT CATCGCAGCG GTCGAGACTA AATGGGAGTA TGTGTTTACA 
GACACAGCAG ATCCAGCCGC AAACCAGAGG AAGTTTGCAT CTGGTGTCCA GAAGTACACC 
AAGGCTGTAT ATACGGAGTA CACCGATTCC ACTTTCACTA CACCCAAGTC AAAACCTCCA 
TGGGCTGGCA TTCAAGGCCC GACTATCAGA ACAGTGGTGA ACGACAGGGT TGTGGTGCAC 
TTCAAAAACT TTGCATCCCA ATCCTTCAGT ATATCTCCCA TCGGCATTCC CTACTGGAAA 
CAATCAGAAG GCGCGGGATA TGAGGACTCC AGCTCTTTTC AGGAGCGTGC GGATGACTCG 
GTGCCCCCTG GTGGCTACTA TAGATACGTG TGGGACATTT CTCCAAACTC TGGGCCGACC 
ACATCTGACC CAGACTGCCT GACCTACATC TACTCATCAC AGGTGGACAT CATTCGAGAC 
TTCAACAGCG GACTCATCGG CGCACTGCTC ATCTGCAAGA CAGACGCTCT AACCGAGGAC 
AGACAGAGGA GAGTGCCAGA GTTCATTCTG CTTCTCTCGG TCTTCGATGA GAGCAAAAGC 
TGGTATCAGG AAGTGGGTCG AGCAGAAAAA CTGAAGAAAA CCTCCAGCAC AAACCAGTAC 
CACACCATCA ACGGCTACAT CAACTCCACA TTACCGGGTC TGAGTGTGTG TGAGAAGAGT 
CCGCAGGTCT CCTGGCACCT CATAGGTCTG GGCTCCAGTC CTGAGATCCA CAGCATCCAG 
TTTGAAGGAC ACACGCTGAA GGTGTTCGAC CACAAGCGGG TGACGGTGGA GATGACACCC 
ATGACCTTCA CCACAGCCCT CATGAAGCCC TCGGCCACGG GGAGGTTTCT GATCTCCTGC 
AGGATCCACT CGCATCAGAC AGCTGGTATG AATGCGTTCT TCACTGTTGA AAACTGTCCT 
GAACCCGCAC CGCTTCCCCT CCCGGACAAG AGAAACGTCA AGCACACTAA TGACGATGAG 
GAGGAGGATG ATGAAGACAA CATGTTCTCT GTGGTGTTCA AACAAGGAGG TCCAATATCT 
GTGCTTCGCT CCAGCGCGAA GGGCCGACCC AAAGTGTGGC TCCATTACAT CTCTGCAGAG 
GAGACGGACT GGGATTATAC CGCTCAGAGC GGAGATCGGC CTGCATCTTC AGCCGGGTCC 
GAGTGGTTCA GAAAAGGCCC TCAGCGTCTG GGTGGTGTTT ATAAGAAAGT GGCGTTCGTG 
GAGTACACAG ACAAGACATT CAGCGTCAAG AAAACCACCG GGAGAACACT GATCGGTCCC 
GAGCTCCGAG GGGAAGTGGG AGACAAGTTT CAGATTGTGT TTAAAAATAT GGCCAGTCGT 
CCATTCAACA TTTATCCAAA CGGCCTGACC AGCGTTCAGC CTCTGAAGAC AACAAACAAA 
GACAAACAGG TAGATCTGCG TTCTCTGGCC GTTCCCCCAG GTGAGATTAT GACATACCTG 
TGGAAGCTGA CGGCTGAAGA TGGACCCACA GATGCCGACC CGCGATGTCT GACGCGGATT 
TACCAGAGTA CGCTGGATCC GGCCCGGGAC GTGGCGTCTG GGCTGGTGGG ACCCCTGATC 
ATCTGCAAAT CCCAGTCTCT GGATAAAAGA GGCAGAGTGG TGACGTCAGA TAAAGAGAAG 
AAGCTGTTGT TTACGGTGTT TGATGAAAAC AGAAGCTGGT ACTTCGAGGA AAACATCAAG 
AGAAACAGTG AAGATCCAGC TAAGATCAAC CGCAATGATC TGGATTTCTA CAATTCTAAC 
ATCATGCACA CTGTGAACGG TTTCATGTTC AACCACCTGC AGTACAAGAT GTGTGTTGGC 
GATGTGATCC TCTGGCATGT GGCGAGCATC GGCATGCAAA ACAACTTCCT GTCAGTTTAT 
TTCACCGGAA ATACATTTGA GCGGGAAAAA GCGTACGGCA CCGTGCTCAC GCTTTTCCCA 
ATGACTGGAG ACACAGTCAC CACTGAAGTG GAGATGATGG GAGAATGGGA GGTCAGTGCA 
TTTGACCCGT CTCTAAAAGA GCGAGGTATG AGCGTCCGCT ATACCGTGGT AAACTGCGAA 
CGTGTGCTCC CCAAAGTAGA CTACGACGAC TACGATGAAG AATTCATGGA GATCATCGAA 
AAAAACTTCC CCAATCCACG AGGCACCATG GGAAAGAACC GCACTATCGC CATCCGCGTC 
TGCAGGAACA GAACTGTGAC GGATTCTGGG AGCAACACGA GACACAATGA GACGCTGAAC 
AGAAGCCGCG TGTGCGAGAT AAGATATGTT CCCGTAAAAG AAGAGAACGA TCTGGAAGCG 
TTATCGCAGG GCGGTATTCC TCAGGATATT CTGGATCAAC TGGACGATGA GATTGGAGGA 
AACTCGGTGG AAAGACAGAA AAGATCCCAC CAGGAATCCC ACACAGTCAC GTCCAGCTAC 
ACTGAATTAC CTCAAAACCA ACACAATGAA ACACAAGCAG GTGCTGACAC TACAAATGAG 
CTGCTCAGAA AATTCCTCTT CAAACATACA AACCTAAACA TCTCTAGAAG AACGCAGGAT 
GAGATGGAGG AAAGCTTGAA TCCGCTTAGC TTTAATACGA TCCGAAATAT CTCAATGGTG 
GAGATTGAAA AAGCAAGGAG TCGGAGAGAA CTGTGGAGCG TTGGGAAAAC TAACCAGAAA 
GATGTTAAAG CCATGAGTGA TAATGAAGTT TTAGAAGACT CTGGAAGAAA GCGGGATAAG 
ATGGAGGAAA GCTTGAACCC GCTGAGCTAT AATTATAAGG ACCTGAAGAA GTATTCGGAG 
ATGGGTTTGG AGCCGGCGTC TAACTCATCG GATGACAAGA TGTACGGGAA GAAGTCGGTG 
ACGAGCGATT ATGATGATTA CAGTGATGAG GGAAGCGTTG TCGGGTTAGA TCATTTTGTT 
GAGGATAATG TCGGCGTTCG GTCGACAGAA TCAAAGTTTA GGAGTTACTA CATCGCGGCT 
GAGGAAGTCA TGTGGGATTA TGGGATTGAT AAACCGGCTC AGCTCATCAA ACCGAGGGAG 
ATGCGGAGGG GACTGAGAAA ATACTTCCCA ACCTATAAAA AAGTGGTGTT TAGAGCGTAT 
CAGGACCGAG ATTTCAGGCA TCCAATCAAA CGTGGAGAAC TGGACGAGCA CCTGGGCATT 
ATGGGTCCTG TAGTGAAAGC AGAGATCAAT GATGTTCTCA CAGTGGTTTT TAAGAACCTG 
GCCTCCAGAC CGTACTCATT CCACTTGCAT GGAGTGTACG ATAAGACGCC GGGGAGTTTC 
AGCACTGGAA TGGGTCCAGA AAACCCCAAG CTGGAGGAAG AAGCAGGAGA TCCAGTTCCT 
CCAGGAGAGG AGAGAGTTTA TAACTGGAGA ATCTCCAGAC GCCAAGGACC CTCCGCCTCA 
GAGTTCGACT GCAAGGCTGG AGCTTATTAC TCCAACGTCA ACATGGAGAA GGACATAAAC 
TCTGGTCTGA TCGGCCCCAT GCTGATTTGT CGTCCTGGAA CGCTCAACCC ACGAGTCCTG 
CTTCAGCCTG ACGTCACAGA TTTCTTCCTT CTCTTCACCA CATTTGACGA AAGAAAGAGC 
TGGTACCTCG ACTACAACAT CAGGAAGTTC TGCATCCCGC CATGCCAGGC CAGAGTAGAC 
GATCCCTGGT TCCAGACTAG CAACAAGTTT GCAGCTATTA ACGGTTACGT GTCTGAGACG 
CTGCCTGGTC TGAGGGTTGA GCAGTACAGG ACGGCCCGCT GGCATCTGCT CAATCTGGGA 
ACTCAAGGCG AGTTTCACGC TGTACACTTC CACGGACTTC CATTCAGAGT GGGAAAGGAT 
CAGGAACACC GCATGGGAAT TTTCAATCTA TATCCTGGTG TTTTTGGGAC GGTGGAGATG 
CGGCCTGCCA TCGCAGGCAC CTGGTTAATA GAGTGCACGA TAGGGGAACA TCAGCTGGCA 
GGAATGAGGG CAAAACTGCT GGTCTATAAC CCACAATGCA ATCAGCCCAT AGGAATGCAA 
TCAAGATGGA TCACCAACAA ACAAATAACA GCGTCTGATT ACTACGGTGA CTGGAAGCCT 
GAATTAGCCA GACTTGAGTT AACTGGATCC ATTAATGCCT GGATGGGCAG CAGCCCAAAC 
TCCTGGCTGC AGGTGGATCT GTTAAGACCC ATGATCCTGC ATGGTATTCA GACTCAGGGT 
GCGAAGACTT CGATGGGTTT GAGCGAGTTC TTCACTATTC TCTACAGCAT CAGTTACAGC 
AACGACCAGG AGAACTGGAG CGTTTACAGA GGAAACAGCA CCAAACCATA TTACACCTTC 
AATGGAAACA TGGACGGTTC CAGAATAAAG GAGAACTTAT TTTCTCCACC CATCGTGGGA 
CGCTACATCC GCATTCACCC TCTGACCTTC CAGAAGCAGC CCACGCTGAG GATTGAACTG 
CTGGGGTGTG ACCTCAACAG CTGTTCTCTG CCACTGGGAA TGGAGCGCAG GTCGATTCCC 
AACAGCAGCA TCAGCGCCTC CTCATTCCTC AATAAGTGGC TGCTGTCCTG GAGCCCCGAC 
CTCGCCAGAA TACACCAGGA GGGACGAGCA AACGCTTGGA GACCAAAGTC TAATAACCCT 
CACGAGTGGC TCCAGGTGGA CTTTGCGTCA GTGAAGCGAG TGACCGGTTT GATCACACAG 
GGGGCTCGGT CCGTCCTGAA GCCCATGATG GTGACAGAAT TCACTGTTTC TGTCAGCAAT 
GATGGACACA GCTGGAGCAG TGTGACGGAG CAGGGCACCG ACAGCGAGCA GATCTTTGAG 
GGCAACAGTG ATAATAATGA GGAGACACTG AACATCTTTG ATCCTCCTCT CTTCACTCGT 
TTCATCCGCG TTCATCCTAA AGGCTGGGTG AACGACATCG CTCTGCGTCT GGAGTTTCTG 
GGCTGCGACA CACAACCAAC ACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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gene@genscript.com

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1-877-436-7274

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f8 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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