SI:DKEYP-27E10.3 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	SI:DKEYP-27E10.3
Entrez Gene ID	100034386
Full Name	si:dkeyp-27e10.3
General protein information	Preferred Names si:dkeyp-27e10.3 Names UPF0606 protein KIAA1549 uncharacterized protein LOC100034386
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017355282.2	XP_017210771.1	UPF0606 protein KIAA1549 isoform X1
XM_017355284.1	XP_017210773.1	UPF0606 protein KIAA1549 homolog isoform X2
XM_021473999.1	XP_021329674.1	UPF0606 protein KIAA1549 isoform X1
XM_017355284.2	XP_017210773.1	UPF0606 protein KIAA1549 isoform X2
XM_021474000.1	XP_021329675.1	UPF0606 protein KIAA1549 isoform X2
XM_017355282.1	XP_017210771.1	UPF0606 protein KIAA1549 homolog isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	KIAA1549	NP_065961.2
Macaca mulatta (Rhesus monkey)	LOC710668	XP_001107649.2
Danio rerio (zebrafish)	si:dkeyp-27e10.3	XP_003198292.2
Gallus gallus (chicken)	KIAA1549	XP_003640459.2
Mus musculus (house mouse)	D630045J12Rik	NP_918950.2
Rattus norvegicus (Norway rat)	RGD1306271	XP_006236401.1
Danio rerio (zebrafish)	si:ch211-1e14.1	XP_005159051.1
Pan troglodytes (chimpanzee)	KIAA1549	XP_001149607.2
Canis lupus familiaris (dog)	KIAA1549	XP_005629795.1

Gene Ontology

The following SI:DKEYP-27E10.3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SI:DKEYP-27E10.3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa146501	XM_021474000.1 Latest version!	Danio rerio si:dkeyp-27e10.3 (si:dkeyp-27e10.3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa58552	XM_017355284.2 Latest version!	Danio rerio si:dkeyp-27e10.3 (si:dkeyp-27e10.3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa146500	XM_021473999.1 Latest version!	Danio rerio si:dkeyp-27e10.3 (si:dkeyp-27e10.3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODa48350	XM_017355282.2 Latest version!	Danio rerio si:dkeyp-27e10.3 (si:dkeyp-27e10.3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
ODa58552	XM_017355284.1	Danio rerio si:dkeyp-27e10.3 (si:dkeyp-27e10.3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa48350	XM_017355282.1	Danio rerio si:dkeyp-27e10.3 (si:dkeyp-27e10.3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SI:DKEYP-27E10.3 ( XM_021474000.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021329675.1 Danio rerio si:dkeyp-27e10.3 (si:dkeyp-27e10.3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa146501
Clone ID Related Accession (Same CDS sequence)	XM_021474000.1
Accession Version	XM_021474000.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4443bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	UPF0606 protein KIAA1549 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395230.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGTT CAAGGATGGT CCCGCGGCAT CTGCTCGGAT GTTGTGTGTT TCTGCTCGCC 
GCTGTGATGC TGGTGGGCTT CACGGTGACA CTCAGCACAC ACAATGCAGC GGAGCTACCT 
TCCAACGTGT CCAGAAGGAG CATCAATCAA CTTCCGGCAA GAACACGCTC ACCTCCTGCC 
TTATCTTCAT CATCCTCATC TGCTCATCGC TCTGAAAGGA CCAGGCGTCC AGCACACACG 
CCTCCAGGTA CCAAACACCC CTCACCATCC CCAGGTCACC ATGACAGTGC CTCCAGCATA 
GATCCCACCC CGTCCCTTGG ATCTGAAGGC TCGGGTAACC TAGGCGATGG GGCCCAAGGC 
ATCCACTTGC TGTTACGTCC ACCAGATCTA CTCACGCCCA GCCAGATGCC ACCTCTGCGG 
GACGACAGAC TCCCTACCCC AGCCCCACCC ACCCTAGTGC CACCCGAAGA CTTATATCCA 
ATCCACTCGG CGGAGATGGA CTGGGGTTCT GGAGACTACC TGGAAACTCT TACATTCATG 
GGATCTGAAG GAGAGGAGTT TTCACTGGTC ACCACATTAC CGACGCATGG ATACGATCCA 
TATGATTTTG ACGAAGATGC CACGGTTAGC TACAACACAG CTTTCCCTTC CCGTCCCATT 
CTTCCTCTTT CATCCAGACA CCTCCAGCCC AGCGCTACCG TAAAATATGG GACTCATTTA 
AGGACCGCCC ATCCTACTGA TCCCCGCCTC CCATTGGCTC CTGATTCTGA TAGAGACGAT 
GATCTGGATT TCGATTGGGC GGAGTTGTTT CCTATTGAGC CAACCGAAAT GCTGCTTCCC 
GACATGAACA GTTTGGAATA CTACAACACG TTACAAGCCA AAGAGAACGC TAGTATTGCG 
AGGAATAAAA CGCATCCTCA TATTACTCCC ACGCACACTT TGGGACCCAC CGACAGTCCT 
ACTGGAACTT TGGGAAAAGT CCCGGATCAG GTTGATGGGA TAACTCATCA ACCAATTCCA 
TCTGAGAAAC CTACAAAACC TCTTGTCCCA TTGAGCACTG AGACCCAGCG ACCTCCAAAA 
GCTCCGGAGA AGGGTGTTCC TTCCATACCT ACATCTCATA TGGGCAAAAC CAAACCATCA 
GTGCCAACAG CGCCCCCTCC AGCCAGCATC TCTGAGGGTC CAGTAATACA AGCCGTCACG 
CTGAAGCCCC CTGCTACAAA ACCCCGTACG ACCACAGTCA AAACCATCTC TAGAACTACA 
ACAACAACAA CAACAACCAC CACTACTACA AATCCCACAA GCCTCTCCAC TAAAAGTGCA 
CCGCCTACAA TTCCCAAAAT TCATCAGCCC ATCGCACCAC GACAGAATGT CTGCAACATC 
AGCAAATCAG AAATGTACCT TGTCCGAGTG GGTATGCCAA GTGGATCATC AGTGGAGTTT 
GCTAAAAATC ATGTTCTTGA GATTCTTCGA CGAGAGTTTA ACCGTTCAGT GGAGCTAAAG 
GTCCACAAAT ATCCACCCAA TTTTATCTTT CGGGCTTTTT CTGGTCCACT GGTCTACACT 
GCTGTGTCAG TCATCAATGC TCTCCGCCAA CCAATGCACA GCACATCGTC TTCTACCATT 
CTCTCTGTTA CAACTATATA TGCAGTGCCT GATTACAAGC ATCAGGTCCA CACAGTGCTG 
CAGTTTGTGC CGGGTCATGT CGATGTGCGT CTGTGTAGCT TTAGCGAGCG AGTGGAAAAG 
GGGCTCCTCA TGGCATATGC GGAAGTGCGC AAACGCTCAC AAGAAAATGG GAATTTCACA 
GTCCATATTG TAAACATAAC TAGTAGCCTT CCGAAGGGTC AGCGTCCGCA GAAGGCTCCA 
GTGGACATCA CGTTTGCTGT GCGTGATTCT CGTGGTTTTC TAACTGGATC GGATGTGAGC 
GGCCACATGA GGCAGCTTAG TGCTGTGGAG TTCAGCTTCT ACCTTGGTTT TCCTACCCTT 
CAGATCGCTG AACCTTTTCA TTATCCTGAA CTCAATGTGT CCCACTTGTT ACGTGCATCC 
TGGGTGAAAA CAGTTTTACT GGGTGTGTTG GATCAGCGTG TGAACGAGCG GACCTTTCAG 
GCCAAAATGG AGCGACGATT AGCATTGCTG GTTGGAGAAG GACTTGGGAT CGGAATTAAC 
AATCGACGAT GGAGAAGATC TACTAGTGTT GGCAACAATA GCGTACAGAT TGTGCGAGCG 
TCTCGACTGG ATGGGCCTGA CTATCCGCTG GAGCTGGTGT ATTTTGTGGA AGAGGGTTCG 
GGAGAGAGAC TGCCAGCCCA GGCCACAGCG GCAATGCTGA ACAGACTGAA CCTGCAGAGA 
GCCGCCATCG TACTGGGATA CCGCGTACAT GGAGTTCTAG CCACACCGGT GGAAAAACTG 
GCCCTGCCGC CGTCTGAGAC TCAGCCCAGT AACGTGTGGC TGATCGTAGG AGTGGTGGTC 
CCAGTGCTGG TGGTGGTCCT CATCATCATC ATCCTCTACT GGAAACTGTG CCGCTCGGAG 
AAGCTGGAGT TTCAGCCAGA CGCCATGAGC ACCATCCAGC AGAGACAGAA GTTGCAGCCT 
CCCAGCGTTA AAGGCTTTGA TTTTGCTAAG CTGCATCTGG GGCAGCATAG TAAGGATGAC 
ATCATGGTAA TTCAGGAACC CGCCCCGTTA CCGCCGTCTG TCAAAGAGGC CACACCCTCA 
GAGGGCGGTG AATTGAACAC GCCTAAATCG AAGAGCTCGT CTACGAAAGC ATCACGTGCA 
GCACGAAGGA GAGGCCGGTT GTCTCCATCT GATGGTGATT CTTTAGGAAG TGAAGCATCC 
AGTGGTAGAG AATCAGCAGA GGAGAGCACC AGACCTGCTG TGACACCCAG TGACAGCAAA 
CCCCAACATC GCAAGAGCAA GCATGCTAAA AATAAACTTG GTGCTCAGCC TGGAAGCGGT 
GTGGATGAGC AGTTGTCCTC ATCGTCCATA TTCGAGCATG TGGACCGTGT GTCTCGAGGC 
TCGTCAGATG GAACACGCAG GGTGTCCAAT AAGATACAGC TTATCGCCAT GCAGCCCATG 
CCGAGCCCAC CCTCACATCC ACATAACCAA CCCCTGAGTC CCAATCTGAC AGACAAGATT 
ACTGACGGAG CACAGGTCAA CAGTGAGATC CAAGTGGCCT TGAGGCATAA ATCTGAGATC 
GAACACCATC GCAATAAGAT CCGCCTGCGT GCCAAACGAA AGGGCCACTA CGAGTTCCCC 
TCCATGGAGG ACATCATGGC AGCGTTCGGC GACAACTCCG AGCAGCAGCG AGTGTATCAA 
CAAGCTCAGG AGCAGATACA CAAGATCTTG CATCCTGACG AGCACACACA CTCGTCGTGC 
GGAGAGCCAC GCAAGAGTTC CAGAGGAAGA CGCTCGCCCA GACACAGGCA GAGGCAGCAG 
AATTTAAGTG GAAGCGGAAG TCCGAGAGAC AAAGATCGCC TCATTTCTGA TCGAGACGCC 
ACCTACAGGA AGTACCCTGG GGTCAACAAC GTAGCCTATG TGTCTGACGC AGATCAGCTC 
CCAGATGCTG GAAGTCCTTC TCCTACTGAT GAGGTGTTTT TAGACCCTGG ATCGCCTCCG 
TCTGGCCCCG CCCCTTCACC GCCCGCTTAC GTCCCCCCTC AGCCAACCAT TGAAGAAGCC 
CGGCAGCAGA TGCACTCCTT ATTGGACGAT GCTTTTGCTC TGGTCTCGCC CTCTTCTCAA 
GGAAGTCCTG CTCCTCTAAC GTTTACTGGC GTTACGGCGG ACACTGGTGT TGGAGGAATC 
AGCCCCGGTC AACCTTCCAG CCCGTCTCCA CGCCCTGCAC GCCAATGGGG CTCCTCTTCT 
TACCCAGCAG CTCCTGCACA CAGTCCTTTC TCCGCTAGGT ATGCAGAGCT AGGAATGTCT 
CCCACATCGG TCCAGGGTTT ACTGCAGAGG CAGACACAGG TCTCTGGGTA TCTGGTGTCT 
GCAGAGATGC AGTCAGATCC TGGGTACTCC AGCAGGGGAC AATATGGAGA CGAGATGCCC 
TCGTCCTCTC GACCACGACC AGTAGGGGGC AGCACAGGTG CGCAGTTGCA TCAGTTGACA 
CAAGTGGGCT TGTCGAGTCG GATTGGATTG TATCCTGGAG TTGGCCGTAG TGTTTCTGGA 
CCCTCAGGAT CAAGCTGGGC TCAGTACCGC TCAGATGAGG AGATCTCTCG ACCAGGATCC 
AACCGAGAGG CTATAATGAC ATTCCCAGAA TACTCTTCTT CACCAGTATT CCAGATGCCC 
AGGACGTCCC TGCAGGATTC TGCACCCCCC CAGTCCCACC TGGAAGCCTC AAACGCGGGT 
TACATCGCCC CAGAAGAGCA CTCTCCACCA CCTCACTCCT CCGCCTCTCT CATCAAGGCC 
ATCAGGGAAG AACTGCAGAG ACTTTCTCAG AAACAAGTTG CTGTCGTCAG CTACCACAGT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329675.1
CDS	492..4934
Translation	MESSRMVPRHLLGCCVFLLAAVMLVGFTVTLSTHNAAELPSNVSRRSINQLPARTRSPPALSSSSSSAHRSERTRRPAHTPPGTKHPSPSPGHHDSASSIDPTPSLGSEGSGNLGDGAQGIHLLLRPPDLLTPSQMPPLRDDRLPTPAPPTLVPPEDLYPIHSAEMDWGSGDYLETLTFMGSEGEEFSLVTTLPTHGYDPYDFDEDATVSYNTAFPSRPILPLSSRHLQPSATVKYGTHLRTAHPTDPRLPLAPDSDRDDDLDFDWAELFPIEPTEMLLPDMNSLEYYNTLQAKENASIARNKTHPHITPTHTLGPTDSPTGTLGKVPDQVDGITHQPIPSEKPTKPLVPLSTETQRPPKAPEKGVPSIPTSHMGKTKPSVPTAPPPASISEGPVIQAVTLKPPATKPRTTTVKTISRTTTTTTTTTTTTNPTSLSTKSAPPTIPKIHQPIAPRQNVCNISKSEMYLVRVGMPSGSSVEFAKNHVLEILRREFNRSVELKVHKYPPNFIFRAFSGPLVYTAVSVINALRQPMHSTSSSTILSVTTIYAVPDYKHQVHTVLQFVPGHVDVRLCSFSERVEKGLLMAYAEVRKRSQENGNFTVHIVNITSSLPKGQRPQKAPVDITFAVRDSRGFLTGSDVSGHMRQLSAVEFSFYLGFPTLQIAEPFHYPELNVSHLLRASWVKTVLLGVLDQRVNERTFQAKMERRLALLVGEGLGIGINNRRWRRSTSVGNNSVQIVRASRLDGPDYPLELVYFVEEGSGERLPAQATAAMLNRLNLQRAAIVLGYRVHGVLATPVEKLALPPSETQPSNVWLIVGVVVPVLVVVLIIIILYWKLCRSEKLEFQPDAMSTIQQRQKLQPPSVKGFDFAKLHLGQHSKDDIMVIQEPAPLPPSVKEATPSEGGELNTPKSKSSSTKASRAARRRGRLSPSDGDSLGSEASSGRESAEESTRPAVTPSDSKPQHRKSKHAKNKLGAQPGSGVDEQLSSSSIFEHVDRVSRGSSDGTRRVSNKIQLIAMQPMPSPPSHPHNQPLSPNLTDKITDGAQVNSEIQVALRHKSEIEHHRNKIRLRAKRKGHYEFPSMEDIMAAFGDNSEQQRVYQQAQEQIHKILHPDEHTHSSCGEPRKSSRGRRSPRHRQRQQNLSGSGSPRDKDRLISDRDATYRKYPGVNNVAYVSDADQLPDAGSPSPTDEVFLDPGSPPSGPAPSPPAYVPPQPTIEEARQQMHSLLDDAFALVSPSSQGSPAPLTFTGVTADTGVGGISPGQPSSPSPRPARQWGSSSYPAAPAHSPFSARYAELGMSPTSVQGLLQRQTQVSGYLVSAEMQSDPGYSSRGQYGDEMPSSSRPRPVGGSTGAQLHQLTQVGLSSRIGLYPGVGRSVSGPSGSSWAQYRSDEEISRPGSNREAIMTFPEYSSSPVFQMPRTSLQDSAPPQSHLEASNAGYIAPEEHSPPPHSSASLIKAIREELQRLSQKQVAVVSYHS

Target ORF information:

RefSeq Version	XM_021474000.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:dkeyp-27e10.3 (si:dkeyp-27e10.3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474000.1

ORF Insert Sequence:

ATGGAGAGTT CAAGGATGGT CCCGCGGCAT CTGCTCGGAT GTTGTGTGTT TCTGCTCGCC 
GCTGTGATGC TGGTGGGCTT CACGGTGACA CTCAGCACAC ACAATGCAGC GGAGCTACCT 
TCCAACGTGT CCAGAAGGAG CATCAATCAA CTTCCGGCAA GAACACGCTC ACCTCCTGCC 
TTATCTTCAT CATCCTCATC TGCTCATCGC TCTGAAAGGA CCAGGCGTCC AGCACACACG 
CCTCCAGGTA CCAAACACCC CTCACCATCC CCAGGTCACC ATGACAGTGC CTCCAGCATA 
GATCCCACCC CGTCCCTTGG ATCTGAAGGC TCGGGTAACC TAGGCGATGG GGCCCAAGGC 
ATCCACTTGC TGTTACGTCC ACCAGATCTA CTCACGCCCA GCCAGATGCC ACCTCTGCGG 
GACGACAGAC TCCCTACCCC AGCCCCACCC ACCCTAGTGC CACCCGAAGA CTTATATCCA 
ATCCACTCGG CGGAGATGGA CTGGGGTTCT GGAGACTACC TGGAAACTCT TACATTCATG 
GGATCTGAAG GAGAGGAGTT TTCACTGGTC ACCACATTAC CGACGCATGG ATACGATCCA 
TATGATTTTG ACGAAGATGC CACGGTTAGC TACAACACAG CTTTCCCTTC CCGTCCCATT 
CTTCCTCTTT CATCCAGACA CCTCCAGCCC AGCGCTACCG TAAAATATGG GACTCATTTA 
AGGACCGCCC ATCCTACTGA TCCCCGCCTC CCATTGGCTC CTGATTCTGA TAGAGACGAT 
GATCTGGATT TCGATTGGGC GGAGTTGTTT CCTATTGAGC CAACCGAAAT GCTGCTTCCC 
GACATGAACA GTTTGGAATA CTACAACACG TTACAAGCCA AAGAGAACGC TAGTATTGCG 
AGGAATAAAA CGCATCCTCA TATTACTCCC ACGCACACTT TGGGACCCAC CGACAGTCCT 
ACTGGAACTT TGGGAAAAGT CCCGGATCAG GTTGATGGGA TAACTCATCA ACCAATTCCA 
TCTGAGAAAC CTACAAAACC TCTTGTCCCA TTGAGCACTG AGACCCAGCG ACCTCCAAAA 
GCTCCGGAGA AGGGTGTTCC TTCCATACCT ACATCTCATA TGGGCAAAAC CAAACCATCA 
GTGCCAACAG CGCCCCCTCC AGCCAGCATC TCTGAGGGTC CAGTAATACA AGCCGTCACG 
CTGAAGCCCC CTGCTACAAA ACCCCGTACG ACCACAGTCA AAACCATCTC TAGAACTACA 
ACAACAACAA CAACAACCAC CACTACTACA AATCCCACAA GCCTCTCCAC TAAAAGTGCA 
CCGCCTACAA TTCCCAAAAT TCATCAGCCC ATCGCACCAC GACAGAATGT CTGCAACATC 
AGCAAATCAG AAATGTACCT TGTCCGAGTG GGTATGCCAA GTGGATCATC AGTGGAGTTT 
GCTAAAAATC ATGTTCTTGA GATTCTTCGA CGAGAGTTTA ACCGTTCAGT GGAGCTAAAG 
GTCCACAAAT ATCCACCCAA TTTTATCTTT CGGGCTTTTT CTGGTCCACT GGTCTACACT 
GCTGTGTCAG TCATCAATGC TCTCCGCCAA CCAATGCACA GCACATCGTC TTCTACCATT 
CTCTCTGTTA CAACTATATA TGCAGTGCCT GATTACAAGC ATCAGGTCCA CACAGTGCTG 
CAGTTTGTGC CGGGTCATGT CGATGTGCGT CTGTGTAGCT TTAGCGAGCG AGTGGAAAAG 
GGGCTCCTCA TGGCATATGC GGAAGTGCGC AAACGCTCAC AAGAAAATGG GAATTTCACA 
GTCCATATTG TAAACATAAC TAGTAGCCTT CCGAAGGGTC AGCGTCCGCA GAAGGCTCCA 
GTGGACATCA CGTTTGCTGT GCGTGATTCT CGTGGTTTTC TAACTGGATC GGATGTGAGC 
GGCCACATGA GGCAGCTTAG TGCTGTGGAG TTCAGCTTCT ACCTTGGTTT TCCTACCCTT 
CAGATCGCTG AACCTTTTCA TTATCCTGAA CTCAATGTGT CCCACTTGTT ACGTGCATCC 
TGGGTGAAAA CAGTTTTACT GGGTGTGTTG GATCAGCGTG TGAACGAGCG GACCTTTCAG 
GCCAAAATGG AGCGACGATT AGCATTGCTG GTTGGAGAAG GACTTGGGAT CGGAATTAAC 
AATCGACGAT GGAGAAGATC TACTAGTGTT GGCAACAATA GCGTACAGAT TGTGCGAGCG 
TCTCGACTGG ATGGGCCTGA CTATCCGCTG GAGCTGGTGT ATTTTGTGGA AGAGGGTTCG 
GGAGAGAGAC TGCCAGCCCA GGCCACAGCG GCAATGCTGA ACAGACTGAA CCTGCAGAGA 
GCCGCCATCG TACTGGGATA CCGCGTACAT GGAGTTCTAG CCACACCGGT GGAAAAACTG 
GCCCTGCCGC CGTCTGAGAC TCAGCCCAGT AACGTGTGGC TGATCGTAGG AGTGGTGGTC 
CCAGTGCTGG TGGTGGTCCT CATCATCATC ATCCTCTACT GGAAACTGTG CCGCTCGGAG 
AAGCTGGAGT TTCAGCCAGA CGCCATGAGC ACCATCCAGC AGAGACAGAA GTTGCAGCCT 
CCCAGCGTTA AAGGCTTTGA TTTTGCTAAG CTGCATCTGG GGCAGCATAG TAAGGATGAC 
ATCATGGTAA TTCAGGAACC CGCCCCGTTA CCGCCGTCTG TCAAAGAGGC CACACCCTCA 
GAGGGCGGTG AATTGAACAC GCCTAAATCG AAGAGCTCGT CTACGAAAGC ATCACGTGCA 
GCACGAAGGA GAGGCCGGTT GTCTCCATCT GATGGTGATT CTTTAGGAAG TGAAGCATCC 
AGTGGTAGAG AATCAGCAGA GGAGAGCACC AGACCTGCTG TGACACCCAG TGACAGCAAA 
CCCCAACATC GCAAGAGCAA GCATGCTAAA AATAAACTTG GTGCTCAGCC TGGAAGCGGT 
GTGGATGAGC AGTTGTCCTC ATCGTCCATA TTCGAGCATG TGGACCGTGT GTCTCGAGGC 
TCGTCAGATG GAACACGCAG GGTGTCCAAT AAGATACAGC TTATCGCCAT GCAGCCCATG 
CCGAGCCCAC CCTCACATCC ACATAACCAA CCCCTGAGTC CCAATCTGAC AGACAAGATT 
ACTGACGGAG CACAGGTCAA CAGTGAGATC CAAGTGGCCT TGAGGCATAA ATCTGAGATC 
GAACACCATC GCAATAAGAT CCGCCTGCGT GCCAAACGAA AGGGCCACTA CGAGTTCCCC 
TCCATGGAGG ACATCATGGC AGCGTTCGGC GACAACTCCG AGCAGCAGCG AGTGTATCAA 
CAAGCTCAGG AGCAGATACA CAAGATCTTG CATCCTGACG AGCACACACA CTCGTCGTGC 
GGAGAGCCAC GCAAGAGTTC CAGAGGAAGA CGCTCGCCCA GACACAGGCA GAGGCAGCAG 
AATTTAAGTG GAAGCGGAAG TCCGAGAGAC AAAGATCGCC TCATTTCTGA TCGAGACGCC 
ACCTACAGGA AGTACCCTGG GGTCAACAAC GTAGCCTATG TGTCTGACGC AGATCAGCTC 
CCAGATGCTG GAAGTCCTTC TCCTACTGAT GAGGTGTTTT TAGACCCTGG ATCGCCTCCG 
TCTGGCCCCG CCCCTTCACC GCCCGCTTAC GTCCCCCCTC AGCCAACCAT TGAAGAAGCC 
CGGCAGCAGA TGCACTCCTT ATTGGACGAT GCTTTTGCTC TGGTCTCGCC CTCTTCTCAA 
GGAAGTCCTG CTCCTCTAAC GTTTACTGGC GTTACGGCGG ACACTGGTGT TGGAGGAATC 
AGCCCCGGTC AACCTTCCAG CCCGTCTCCA CGCCCTGCAC GCCAATGGGG CTCCTCTTCT 
TACCCAGCAG CTCCTGCACA CAGTCCTTTC TCCGCTAGGT ATGCAGAGCT AGGAATGTCT 
CCCACATCGG TCCAGGGTTT ACTGCAGAGG CAGACACAGG TCTCTGGGTA TCTGGTGTCT 
GCAGAGATGC AGTCAGATCC TGGGTACTCC AGCAGGGGAC AATATGGAGA CGAGATGCCC 
TCGTCCTCTC GACCACGACC AGTAGGGGGC AGCACAGGTG CGCAGTTGCA TCAGTTGACA 
CAAGTGGGCT TGTCGAGTCG GATTGGATTG TATCCTGGAG TTGGCCGTAG TGTTTCTGGA 
CCCTCAGGAT CAAGCTGGGC TCAGTACCGC TCAGATGAGG AGATCTCTCG ACCAGGATCC 
AACCGAGAGG CTATAATGAC ATTCCCAGAA TACTCTTCTT CACCAGTATT CCAGATGCCC 
AGGACGTCCC TGCAGGATTC TGCACCCCCC CAGTCCCACC TGGAAGCCTC AAACGCGGGT 
TACATCGCCC CAGAAGAGCA CTCTCCACCA CCTCACTCCT CCGCCTCTCT CATCAAGGCC 
ATCAGGGAAG AACTGCAGAG ACTTTCTCAG AAACAAGTTG CTGTCGTCAG CTACCACAGT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:DKEYP-27E10.3 ( XM_017355284.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017210773.1 Danio rerio si:dkeyp-27e10.3 (si:dkeyp-27e10.3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa58552
Clone ID Related Accession (Same CDS sequence)	XM_017355284.1 , XM_017355284.2
Accession Version	XM_017355284.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4443bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	UPF0606 protein KIAA1549 homolog isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGTT CAAGGATGGT CCCGCGGCAT CTGCTCGGAT GTTGTGTGTT TCTGCTCGCC 
GCTGTGATGC TGGTGGGCTT CACGGTGACA CTCAGCACAC ACAATGCAGC GGAGCTACCT 
TCCAACGTGT CCAGAAGGAG CATCAATCAA CTTCCGGCAA GAACACGCTC ACCTCCTGCC 
TTATCTTCAT CATCCTCATC TGCTCATCGC TCTGAAAGGA CCAGGCGTCC AGCACACACG 
CCTCCAGGTA CCAAACACCC CTCACCATCC CCAGGTCACC ATGACAGTGC CTCCAGCATA 
GATCCCACCC CGTCCCTTGG ATCTGAAGGC TCGGGTAACC TAGGCGATGG GGCCCAAGGC 
ATCCACTTGC TGTTACGTCC ACCAGATCTA CTCACGCCCA GCCAGATGCC ACCTCTGCGG 
GACGACAGAC TCCCTACCCC AGCCCCACCC ACCCTAGTGC CACCCGAAGA CTTATATCCA 
ATCCACTCGG CGGAGATGGA CTGGGGTTCT GGAGACTACC TGGAAACTCT TACATTCATG 
GGATCTGAAG GAGAGGAGTT TTCACTGGTC ACCACATTAC CGACGCATGG ATACGATCCA 
TATGATTTTG ACGAAGATGC CACGGTTAGC TACAACACAG CTTTCCCTTC CCGTCCCATT 
CTTCCTCTTT CATCCAGACA CCTCCAGCCC AGCGCTACCG TAAAATATGG GACTCATTTA 
AGGACCGCCC ATCCTACTGA TCCCCGCCTC CCATTGGCTC CTGATTCTGA TAGAGACGAT 
GATCTGGATT TCGATTGGGC GGAGTTGTTT CCTATTGAGC CAACCGAAAT GCTGCTTCCC 
GACATGAACA GTTTGGAATA CTACAACACG TTACAAGCCA AAGAGAACGC TAGTATTGCG 
AGGAATAAAA CGCATCCTCA TATTACTCCC ACGCACACTT TGGGACCCAC CGACAGTCCT 
ACTGGAACTT TGGGAAAAGT CCCGGATCAG GTTGATGGGA TAACTCATCA ACCAATTCCA 
TCTGAGAAAC CTACAAAACC TCTTGTCCCA TTGAGCACTG AGACCCAGCG ACCTCCAAAA 
GCTCCGGAGA AGGGTGTTCC TTCCATACCT ACATCTCATA TGGGCAAAAC CAAACCATCA 
GTGCCAACAG CGCCCCCTCC AGCCAGCATC TCTGAGGGTC CAGTAATACA AGCCGTCACG 
CTGAAGCCCC CTGCTACAAA ACCCCGTACG ACCACAGTCA AAACCATCTC TAGAACTACA 
ACAACAACAA CAACAACCAC CACTACTACA AATCCCACAA GCCTCTCCAC TAAAAGTGCA 
CCGCCTACAA TTCCCAAAAT TCATCAGCCC ATCGCACCAC GACAGAATGT CTGCAACATC 
AGCAAATCAG AAATGTACCT TGTCCGAGTG GGTATGCCAA GTGGATCATC AGTGGAGTTT 
GCTAAAAATC ATGTTCTTGA GATTCTTCGA CGAGAGTTTA ACCGTTCAGT GGAGCTAAAG 
GTCCACAAAT ATCCACCCAA TTTTATCTTT CGGGCTTTTT CTGGTCCACT GGTCTACACT 
GCTGTGTCAG TCATCAATGC TCTCCGCCAA CCAATGCACA GCACATCGTC TTCTACCATT 
CTCTCTGTTA CAACTATATA TGCAGTGCCT GATTACAAGC ATCAGGTCCA CACAGTGCTG 
CAGTTTGTGC CAGGTCATGT GGATGTGCGT CTGTGTAGCT TTAGCGAGCG AGTGGAAAAG 
GGGCTCCTCA TGGCATATGC GGAAGTGCGC AAACGCTCAC AAGAAAATGG GAATTTCACA 
GTCCATATTG TGAACATAAC TAGTAGCCTT CCCAAGGGTC AGCGTCCGCA GAAGGCTCCA 
GTGGACATCA CGTTTGCTGT GCGTGATTCT CGTGGTTTTC TAACTGGATC GGATGTGAGC 
GGCCACATGA GGCAGCTTAG TGCTGTGGAG TTCAGCTTCT ACCTTGGTTT TCCTACCCTA 
CAGATCGCTG AACCTTTTCA TTATCCTGAA CTCAATGTGT CCCACTTGTT ACGTGCATCC 
TGGGTGAAAA CAGTTTTACT GGGTGTGTTG GATCAGCGTG TGAACGAGCG GACCTTTCAG 
GCCAAAATGG AGCGACGATT AGCATTGCTG GTTGGAGAAG GACTTGGGAT CGGAATTAAC 
AATCGACGAT GGAGAAGATC TACTAGTGTT GGCAACAATA GCGTACAGAT TGTGCGAGCG 
TCTCGACTGG ATGGGCCTGA CTATCCGCTG GAGCTGGTGT ATTTTGTGGA AGAGGGTTCG 
GGAGAGAGAC TGCCAGCCCA GGCCACAGCG GCAATGCTGA ACAGACTGAA CCTGCAGAGA 
GCCGCCATCG TACTGGGATA CCGCGTACAC GGAGTTCTAG CCACACCGGT GGAAAAACTG 
GCCCTGCCGC CGTCTGAGAC TCAGCCCAGT AACGTGTGGC TGATCGTAGG AGTGGTGGTC 
CCAGTGCTGG TGGTGGTCCT CATCATCATC ATCCTCTACT GGAAACTGTG CCGCTCGGAG 
AAGCTGGAGT TTCAGCCAGA CGCCATGAGC ACCATCCAGC AGAGACAGAA GTTGCAGCCT 
CCCAGCGTTA AAGGCTTTGA TTTTGCTAAG CTGCATCTGG GGCAGCATAG TAAGGATGAC 
ATCATGGTAA TTCAGGAACC CGCCCCGTTA CCGCCGTCTG TCAAAGAGGC CACACCCTCA 
GAGGGCGGTG AATTGAACAC GCCTAAATCG AAGAGCTCGT CTACGAAAGC ATCACGTGCA 
GCACGAAGGA GAGGCCGGTT GTCTCCATCT GATGGTGATT CTTTAGGAAG TGAAGCATCT 
AGTGGTAGAG AATCAGCAGA GGAGAGCACC AGACCTGCTG TGACACCCAG TGACAGCAAA 
CCCCAACATC GAAAGAGCAA GCATGCTAAA AATAAACTTG GTGCTCAGCC TGGAAGCGGT 
GTGGATGAGC AGTTGTCCTC ATCGTCCATA TTCGAGCATG TGGACCGTGT GTCTCGAGGC 
TCGTCCGATG GAACACGCAG GGTGTCCAAT AAGATACAGC TTATCGCCAT GCAGCCCATG 
CCGAGCCCAC CCTCACATCC ACATAACCAA CCCCTGAGTC CCAATCTGAC AGACAAGATT 
ACTGACGGAG CAAAGGTCAA CAGTGAGATC CAAGTGGCCT TGAGGCATAA ATCTGAGATC 
GAACACCATC GCAATAAGAT CCGCCTGCGT GCCAAACGAA AGGGCCACTA CGAGTTCCCC 
TCCATGGAGG ACATCATGGC AGCGTTCGGC GACAACTCCG AGCAGCAGCG AGTGTATCAA 
CAAGCTCAGG AGCAGATACA CAAGATCTTG CATCCTGACG AGCACACACA CTCGTCGTGC 
GGAGAGCCAC GCAAGAGTTC CAGAGGAAGA CGCTCACCCA GACACAGGCA GAGGCAGCAG 
AATTTAAGTG GAAGCGGAAG TCCGAGAGAC AAAGATCGCC TCATTTCTGA TCGAGACGCC 
ACCTACAGGA AGTACCCTGG GGTCAACAAC GTAGCCTATG TGTCTGACGC AGATCAGCTC 
CCAGATGCTG GAAGTCCTTC TCCTACTGAT GAGGTGTTTT TAGACCCTGG ATCGCCTCCG 
TCTGGCCCCG CCCCTTCACC GCCCGCTTAC GTCCCCCCTC AGCCAACCAT TGAAGAAGCC 
CGGCAGCAGA TGCACTCCTT ATTGGACGAT GCTTTTGCTC TGGTCTCGCC CTCTTCTCAA 
GGAAGTCCTG CTCCTCTAAC GTTTACTGGC GTTACGGCGG ACACTGGTGT TGGAGGAATC 
AGCCCCGGTC AACCTTCCAG CCCGTCTCCA CGCCCTGCAC GCCAATGGGG CTCCTCTTCT 
TACCCAGCAG CTCCTGCACA CAGTCCTTTC TCCGCTAGGT ATGCAGAGCT AGGAATGTCT 
CCCACATCGG TCCAGGGTTT ACTGCAGAGG CAGACACAGG TCTCTGGGTA TCTGGTGTCT 
GCAGAGATGC AGTCAGATCC TGGGTATTCC AGCAGGGGAC AATATGGAGA CGAGATGCCC 
TCGTCCTCTC GACCACGACC AGTAGGGGGC AGCACAGGTG CGCAGTTGCA TCAGTTGACA 
CAAGTGGGCT TGTCGAGTCG GATTGGATTG TATCCTGGAG TTGGCCGTAG TGTTTCTGGA 
CCCTCAGGAT CAAGCTGGGC TCAGTACCGC TCAGATGAGG AGATCTCTCG ACCAGGATCC 
AACCGAGAGG CTATAATGAC ATTCCCAGAA TACTCTTCTT CACCAGTATT CCAGATGCCC 
AGGACGTCCC TGCAGGATTC TGCACCCCCC CAGTCCCACC TGGAAGCCTC AAACGCGGGT 
TACATCGCCC CAGAAGAGCA CTCTCCACCA CCTCACTCCT CCGCCTCTCT CATCAAGGCC 
ATCAGGGAAG AACTGCAGAG ACTTTCTCAG AAACAAGTTG CTGTCGTCAG CTACCACAGT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210773.1
CDS	930..5372
Translation	MESSRMVPRHLLGCCVFLLAAVMLVGFTVTLSTHNAAELPSNVSRRSINQLPARTRSPPALSSSSSSAHRSERTRRPAHTPPGTKHPSPSPGHHDSASSIDPTPSLGSEGSGNLGDGAQGIHLLLRPPDLLTPSQMPPLRDDRLPTPAPPTLVPPEDLYPIHSAEMDWGSGDYLETLTFMGSEGEEFSLVTTLPTHGYDPYDFDEDATVSYNTAFPSRPILPLSSRHLQPSATVKYGTHLRTAHPTDPRLPLAPDSDRDDDLDFDWAELFPIEPTEMLLPDMNSLEYYNTLQAKENASIARNKTHPHITPTHTLGPTDSPTGTLGKVPDQVDGITHQPIPSEKPTKPLVPLSTETQRPPKAPEKGVPSIPTSHMGKTKPSVPTAPPPASISEGPVIQAVTLKPPATKPRTTTVKTISRTTTTTTTTTTTTNPTSLSTKSAPPTIPKIHQPIAPRQNVCNISKSEMYLVRVGMPSGSSVEFAKNHVLEILRREFNRSVELKVHKYPPNFIFRAFSGPLVYTAVSVINALRQPMHSTSSSTILSVTTIYAVPDYKHQVHTVLQFVPGHVDVRLCSFSERVEKGLLMAYAEVRKRSQENGNFTVHIVNITSSLPKGQRPQKAPVDITFAVRDSRGFLTGSDVSGHMRQLSAVEFSFYLGFPTLQIAEPFHYPELNVSHLLRASWVKTVLLGVLDQRVNERTFQAKMERRLALLVGEGLGIGINNRRWRRSTSVGNNSVQIVRASRLDGPDYPLELVYFVEEGSGERLPAQATAAMLNRLNLQRAAIVLGYRVHGVLATPVEKLALPPSETQPSNVWLIVGVVVPVLVVVLIIIILYWKLCRSEKLEFQPDAMSTIQQRQKLQPPSVKGFDFAKLHLGQHSKDDIMVIQEPAPLPPSVKEATPSEGGELNTPKSKSSSTKASRAARRRGRLSPSDGDSLGSEASSGRESAEESTRPAVTPSDSKPQHRKSKHAKNKLGAQPGSGVDEQLSSSSIFEHVDRVSRGSSDGTRRVSNKIQLIAMQPMPSPPSHPHNQPLSPNLTDKITDGAKVNSEIQVALRHKSEIEHHRNKIRLRAKRKGHYEFPSMEDIMAAFGDNSEQQRVYQQAQEQIHKILHPDEHTHSSCGEPRKSSRGRRSPRHRQRQQNLSGSGSPRDKDRLISDRDATYRKYPGVNNVAYVSDADQLPDAGSPSPTDEVFLDPGSPPSGPAPSPPAYVPPQPTIEEARQQMHSLLDDAFALVSPSSQGSPAPLTFTGVTADTGVGGISPGQPSSPSPRPARQWGSSSYPAAPAHSPFSARYAELGMSPTSVQGLLQRQTQVSGYLVSAEMQSDPGYSSRGQYGDEMPSSSRPRPVGGSTGAQLHQLTQVGLSSRIGLYPGVGRSVSGPSGSSWAQYRSDEEISRPGSNREAIMTFPEYSSSPVFQMPRTSLQDSAPPQSHLEASNAGYIAPEEHSPPPHSSASLIKAIREELQRLSQKQVAVVSYHS

Target ORF information:

RefSeq Version	XM_017355284.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:dkeyp-27e10.3 (si:dkeyp-27e10.3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017355284.1

ORF Insert Sequence:

ATGGAGAGTT CAAGGATGGT CCCGCGGCAT CTGCTCGGAT GTTGTGTGTT TCTGCTCGCC 
GCTGTGATGC TGGTGGGCTT CACGGTGACA CTCAGCACAC ACAATGCAGC GGAGCTACCT 
TCCAACGTGT CCAGAAGGAG CATCAATCAA CTTCCGGCAA GAACACGCTC ACCTCCTGCC 
TTATCTTCAT CATCCTCATC TGCTCATCGC TCTGAAAGGA CCAGGCGTCC AGCACACACG 
CCTCCAGGTA CCAAACACCC CTCACCATCC CCAGGTCACC ATGACAGTGC CTCCAGCATA 
GATCCCACCC CGTCCCTTGG ATCTGAAGGC TCGGGTAACC TAGGCGATGG GGCCCAAGGC 
ATCCACTTGC TGTTACGTCC ACCAGATCTA CTCACGCCCA GCCAGATGCC ACCTCTGCGG 
GACGACAGAC TCCCTACCCC AGCCCCACCC ACCCTAGTGC CACCCGAAGA CTTATATCCA 
ATCCACTCGG CGGAGATGGA CTGGGGTTCT GGAGACTACC TGGAAACTCT TACATTCATG 
GGATCTGAAG GAGAGGAGTT TTCACTGGTC ACCACATTAC CGACGCATGG ATACGATCCA 
TATGATTTTG ACGAAGATGC CACGGTTAGC TACAACACAG CTTTCCCTTC CCGTCCCATT 
CTTCCTCTTT CATCCAGACA CCTCCAGCCC AGCGCTACCG TAAAATATGG GACTCATTTA 
AGGACCGCCC ATCCTACTGA TCCCCGCCTC CCATTGGCTC CTGATTCTGA TAGAGACGAT 
GATCTGGATT TCGATTGGGC GGAGTTGTTT CCTATTGAGC CAACCGAAAT GCTGCTTCCC 
GACATGAACA GTTTGGAATA CTACAACACG TTACAAGCCA AAGAGAACGC TAGTATTGCG 
AGGAATAAAA CGCATCCTCA TATTACTCCC ACGCACACTT TGGGACCCAC CGACAGTCCT 
ACTGGAACTT TGGGAAAAGT CCCGGATCAG GTTGATGGGA TAACTCATCA ACCAATTCCA 
TCTGAGAAAC CTACAAAACC TCTTGTCCCA TTGAGCACTG AGACCCAGCG ACCTCCAAAA 
GCTCCGGAGA AGGGTGTTCC TTCCATACCT ACATCTCATA TGGGCAAAAC CAAACCATCA 
GTGCCAACAG CGCCCCCTCC AGCCAGCATC TCTGAGGGTC CAGTAATACA AGCCGTCACG 
CTGAAGCCCC CTGCTACAAA ACCCCGTACG ACCACAGTCA AAACCATCTC TAGAACTACA 
ACAACAACAA CAACAACCAC CACTACTACA AATCCCACAA GCCTCTCCAC TAAAAGTGCA 
CCGCCTACAA TTCCCAAAAT TCATCAGCCC ATCGCACCAC GACAGAATGT CTGCAACATC 
AGCAAATCAG AAATGTACCT TGTCCGAGTG GGTATGCCAA GTGGATCATC AGTGGAGTTT 
GCTAAAAATC ATGTTCTTGA GATTCTTCGA CGAGAGTTTA ACCGTTCAGT GGAGCTAAAG 
GTCCACAAAT ATCCACCCAA TTTTATCTTT CGGGCTTTTT CTGGTCCACT GGTCTACACT 
GCTGTGTCAG TCATCAATGC TCTCCGCCAA CCAATGCACA GCACATCGTC TTCTACCATT 
CTCTCTGTTA CAACTATATA TGCAGTGCCT GATTACAAGC ATCAGGTCCA CACAGTGCTG 
CAGTTTGTGC CAGGTCATGT GGATGTGCGT CTGTGTAGCT TTAGCGAGCG AGTGGAAAAG 
GGGCTCCTCA TGGCATATGC GGAAGTGCGC AAACGCTCAC AAGAAAATGG GAATTTCACA 
GTCCATATTG TGAACATAAC TAGTAGCCTT CCCAAGGGTC AGCGTCCGCA GAAGGCTCCA 
GTGGACATCA CGTTTGCTGT GCGTGATTCT CGTGGTTTTC TAACTGGATC GGATGTGAGC 
GGCCACATGA GGCAGCTTAG TGCTGTGGAG TTCAGCTTCT ACCTTGGTTT TCCTACCCTA 
CAGATCGCTG AACCTTTTCA TTATCCTGAA CTCAATGTGT CCCACTTGTT ACGTGCATCC 
TGGGTGAAAA CAGTTTTACT GGGTGTGTTG GATCAGCGTG TGAACGAGCG GACCTTTCAG 
GCCAAAATGG AGCGACGATT AGCATTGCTG GTTGGAGAAG GACTTGGGAT CGGAATTAAC 
AATCGACGAT GGAGAAGATC TACTAGTGTT GGCAACAATA GCGTACAGAT TGTGCGAGCG 
TCTCGACTGG ATGGGCCTGA CTATCCGCTG GAGCTGGTGT ATTTTGTGGA AGAGGGTTCG 
GGAGAGAGAC TGCCAGCCCA GGCCACAGCG GCAATGCTGA ACAGACTGAA CCTGCAGAGA 
GCCGCCATCG TACTGGGATA CCGCGTACAC GGAGTTCTAG CCACACCGGT GGAAAAACTG 
GCCCTGCCGC CGTCTGAGAC TCAGCCCAGT AACGTGTGGC TGATCGTAGG AGTGGTGGTC 
CCAGTGCTGG TGGTGGTCCT CATCATCATC ATCCTCTACT GGAAACTGTG CCGCTCGGAG 
AAGCTGGAGT TTCAGCCAGA CGCCATGAGC ACCATCCAGC AGAGACAGAA GTTGCAGCCT 
CCCAGCGTTA AAGGCTTTGA TTTTGCTAAG CTGCATCTGG GGCAGCATAG TAAGGATGAC 
ATCATGGTAA TTCAGGAACC CGCCCCGTTA CCGCCGTCTG TCAAAGAGGC CACACCCTCA 
GAGGGCGGTG AATTGAACAC GCCTAAATCG AAGAGCTCGT CTACGAAAGC ATCACGTGCA 
GCACGAAGGA GAGGCCGGTT GTCTCCATCT GATGGTGATT CTTTAGGAAG TGAAGCATCT 
AGTGGTAGAG AATCAGCAGA GGAGAGCACC AGACCTGCTG TGACACCCAG TGACAGCAAA 
CCCCAACATC GAAAGAGCAA GCATGCTAAA AATAAACTTG GTGCTCAGCC TGGAAGCGGT 
GTGGATGAGC AGTTGTCCTC ATCGTCCATA TTCGAGCATG TGGACCGTGT GTCTCGAGGC 
TCGTCCGATG GAACACGCAG GGTGTCCAAT AAGATACAGC TTATCGCCAT GCAGCCCATG 
CCGAGCCCAC CCTCACATCC ACATAACCAA CCCCTGAGTC CCAATCTGAC AGACAAGATT 
ACTGACGGAG CAAAGGTCAA CAGTGAGATC CAAGTGGCCT TGAGGCATAA ATCTGAGATC 
GAACACCATC GCAATAAGAT CCGCCTGCGT GCCAAACGAA AGGGCCACTA CGAGTTCCCC 
TCCATGGAGG ACATCATGGC AGCGTTCGGC GACAACTCCG AGCAGCAGCG AGTGTATCAA 
CAAGCTCAGG AGCAGATACA CAAGATCTTG CATCCTGACG AGCACACACA CTCGTCGTGC 
GGAGAGCCAC GCAAGAGTTC CAGAGGAAGA CGCTCACCCA GACACAGGCA GAGGCAGCAG 
AATTTAAGTG GAAGCGGAAG TCCGAGAGAC AAAGATCGCC TCATTTCTGA TCGAGACGCC 
ACCTACAGGA AGTACCCTGG GGTCAACAAC GTAGCCTATG TGTCTGACGC AGATCAGCTC 
CCAGATGCTG GAAGTCCTTC TCCTACTGAT GAGGTGTTTT TAGACCCTGG ATCGCCTCCG 
TCTGGCCCCG CCCCTTCACC GCCCGCTTAC GTCCCCCCTC AGCCAACCAT TGAAGAAGCC 
CGGCAGCAGA TGCACTCCTT ATTGGACGAT GCTTTTGCTC TGGTCTCGCC CTCTTCTCAA 
GGAAGTCCTG CTCCTCTAAC GTTTACTGGC GTTACGGCGG ACACTGGTGT TGGAGGAATC 
AGCCCCGGTC AACCTTCCAG CCCGTCTCCA CGCCCTGCAC GCCAATGGGG CTCCTCTTCT 
TACCCAGCAG CTCCTGCACA CAGTCCTTTC TCCGCTAGGT ATGCAGAGCT AGGAATGTCT 
CCCACATCGG TCCAGGGTTT ACTGCAGAGG CAGACACAGG TCTCTGGGTA TCTGGTGTCT 
GCAGAGATGC AGTCAGATCC TGGGTATTCC AGCAGGGGAC AATATGGAGA CGAGATGCCC 
TCGTCCTCTC GACCACGACC AGTAGGGGGC AGCACAGGTG CGCAGTTGCA TCAGTTGACA 
CAAGTGGGCT TGTCGAGTCG GATTGGATTG TATCCTGGAG TTGGCCGTAG TGTTTCTGGA 
CCCTCAGGAT CAAGCTGGGC TCAGTACCGC TCAGATGAGG AGATCTCTCG ACCAGGATCC 
AACCGAGAGG CTATAATGAC ATTCCCAGAA TACTCTTCTT CACCAGTATT CCAGATGCCC 
AGGACGTCCC TGCAGGATTC TGCACCCCCC CAGTCCCACC TGGAAGCCTC AAACGCGGGT 
TACATCGCCC CAGAAGAGCA CTCTCCACCA CCTCACTCCT CCGCCTCTCT CATCAAGGCC 
ATCAGGGAAG AACTGCAGAG ACTTTCTCAG AAACAAGTTG CTGTCGTCAG CTACCACAGT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:DKEYP-27E10.3 ( XM_017355284.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017210773.1 Danio rerio si:dkeyp-27e10.3 (si:dkeyp-27e10.3), transcript variant X2, mRNA.

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CloneID	ODa58552
Clone ID Related Accession (Same CDS sequence)	XM_017355284.1 , XM_017355284.2
Accession Version	XM_017355284.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4443bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	UPF0606 protein KIAA1549 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017355284.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGTT CAAGGATGGT CCCGCGGCAT CTGCTCGGAT GTTGTGTGTT TCTGCTCGCC 
GCTGTGATGC TGGTGGGCTT CACGGTGACA CTCAGCACAC ACAATGCAGC GGAGCTACCT 
TCCAACGTGT CCAGAAGGAG CATCAATCAA CTTCCGGCAA GAACACGCTC ACCTCCTGCC 
TTATCTTCAT CATCCTCATC TGCTCATCGC TCTGAAAGGA CCAGGCGTCC AGCACACACG 
CCTCCAGGTA CCAAACACCC CTCACCATCC CCAGGTCACC ATGACAGTGC CTCCAGCATA 
GATCCCACCC CGTCCCTTGG ATCTGAAGGC TCGGGTAACC TAGGCGATGG GGCCCAAGGC 
ATCCACTTGC TGTTACGTCC ACCAGATCTA CTCACGCCCA GCCAGATGCC ACCTCTGCGG 
GACGACAGAC TCCCTACCCC AGCCCCACCC ACCCTAGTGC CACCCGAAGA CTTATATCCA 
ATCCACTCGG CGGAGATGGA CTGGGGTTCT GGAGACTACC TGGAAACTCT TACATTCATG 
GGATCTGAAG GAGAGGAGTT TTCACTGGTC ACCACATTAC CGACGCATGG ATACGATCCA 
TATGATTTTG ACGAAGATGC CACGGTTAGC TACAACACAG CTTTCCCTTC CCGTCCCATT 
CTTCCTCTTT CATCCAGACA CCTCCAGCCC AGCGCTACCG TAAAATATGG GACTCATTTA 
AGGACCGCCC ATCCTACTGA TCCCCGCCTC CCATTGGCTC CTGATTCTGA TAGAGACGAT 
GATCTGGATT TCGATTGGGC GGAGTTGTTT CCTATTGAGC CAACCGAAAT GCTGCTTCCC 
GACATGAACA GTTTGGAATA CTACAACACG TTACAAGCCA AAGAGAACGC TAGTATTGCG 
AGGAATAAAA CGCATCCTCA TATTACTCCC ACGCACACTT TGGGACCCAC CGACAGTCCT 
ACTGGAACTT TGGGAAAAGT CCCGGATCAG GTTGATGGGA TAACTCATCA ACCAATTCCA 
TCTGAGAAAC CTACAAAACC TCTTGTCCCA TTGAGCACTG AGACCCAGCG ACCTCCAAAA 
GCTCCGGAGA AGGGTGTTCC TTCCATACCT ACATCTCATA TGGGCAAAAC CAAACCATCA 
GTGCCAACAG CGCCCCCTCC AGCCAGCATC TCTGAGGGTC CAGTAATACA AGCCGTCACG 
CTGAAGCCCC CTGCTACAAA ACCCCGTACG ACCACAGTCA AAACCATCTC TAGAACTACA 
ACAACAACAA CAACAACCAC CACTACTACA AATCCCACAA GCCTCTCCAC TAAAAGTGCA 
CCGCCTACAA TTCCCAAAAT TCATCAGCCC ATCGCACCAC GACAGAATGT CTGCAACATC 
AGCAAATCAG AAATGTACCT TGTCCGAGTG GGTATGCCAA GTGGATCATC AGTGGAGTTT 
GCTAAAAATC ATGTTCTTGA GATTCTTCGA CGAGAGTTTA ACCGTTCAGT GGAGCTAAAG 
GTCCACAAAT ATCCACCCAA TTTTATCTTT CGGGCTTTTT CTGGTCCACT GGTCTACACT 
GCTGTGTCAG TCATCAATGC TCTCCGCCAA CCAATGCACA GCACATCGTC TTCTACCATT 
CTCTCTGTTA CAACTATATA TGCAGTGCCT GATTACAAGC ATCAGGTCCA CACAGTGCTG 
CAGTTTGTGC CAGGTCATGT GGATGTGCGT CTGTGTAGCT TTAGCGAGCG AGTGGAAAAG 
GGGCTCCTCA TGGCATATGC GGAAGTGCGC AAACGCTCAC AAGAAAATGG GAATTTCACA 
GTCCATATTG TGAACATAAC TAGTAGCCTT CCCAAGGGTC AGCGTCCGCA GAAGGCTCCA 
GTGGACATCA CGTTTGCTGT GCGTGATTCT CGTGGTTTTC TAACTGGATC GGATGTGAGC 
GGCCACATGA GGCAGCTTAG TGCTGTGGAG TTCAGCTTCT ACCTTGGTTT TCCTACCCTA 
CAGATCGCTG AACCTTTTCA TTATCCTGAA CTCAATGTGT CCCACTTGTT ACGTGCATCC 
TGGGTGAAAA CAGTTTTACT GGGTGTGTTG GATCAGCGTG TGAACGAGCG GACCTTTCAG 
GCCAAAATGG AGCGACGATT AGCATTGCTG GTTGGAGAAG GACTTGGGAT CGGAATTAAC 
AATCGACGAT GGAGAAGATC TACTAGTGTT GGCAACAATA GCGTACAGAT TGTGCGAGCG 
TCTCGACTGG ATGGGCCTGA CTATCCGCTG GAGCTGGTGT ATTTTGTGGA AGAGGGTTCG 
GGAGAGAGAC TGCCAGCCCA GGCCACAGCG GCAATGCTGA ACAGACTGAA CCTGCAGAGA 
GCCGCCATCG TACTGGGATA CCGCGTACAC GGAGTTCTAG CCACACCGGT GGAAAAACTG 
GCCCTGCCGC CGTCTGAGAC TCAGCCCAGT AACGTGTGGC TGATCGTAGG AGTGGTGGTC 
CCAGTGCTGG TGGTGGTCCT CATCATCATC ATCCTCTACT GGAAACTGTG CCGCTCGGAG 
AAGCTGGAGT TTCAGCCAGA CGCCATGAGC ACCATCCAGC AGAGACAGAA GTTGCAGCCT 
CCCAGCGTTA AAGGCTTTGA TTTTGCTAAG CTGCATCTGG GGCAGCATAG TAAGGATGAC 
ATCATGGTAA TTCAGGAACC CGCCCCGTTA CCGCCGTCTG TCAAAGAGGC CACACCCTCA 
GAGGGCGGTG AATTGAACAC GCCTAAATCG AAGAGCTCGT CTACGAAAGC ATCACGTGCA 
GCACGAAGGA GAGGCCGGTT GTCTCCATCT GATGGTGATT CTTTAGGAAG TGAAGCATCT 
AGTGGTAGAG AATCAGCAGA GGAGAGCACC AGACCTGCTG TGACACCCAG TGACAGCAAA 
CCCCAACATC GAAAGAGCAA GCATGCTAAA AATAAACTTG GTGCTCAGCC TGGAAGCGGT 
GTGGATGAGC AGTTGTCCTC ATCGTCCATA TTCGAGCATG TGGACCGTGT GTCTCGAGGC 
TCGTCCGATG GAACACGCAG GGTGTCCAAT AAGATACAGC TTATCGCCAT GCAGCCCATG 
CCGAGCCCAC CCTCACATCC ACATAACCAA CCCCTGAGTC CCAATCTGAC AGACAAGATT 
ACTGACGGAG CAAAGGTCAA CAGTGAGATC CAAGTGGCCT TGAGGCATAA ATCTGAGATC 
GAACACCATC GCAATAAGAT CCGCCTGCGT GCCAAACGAA AGGGCCACTA CGAGTTCCCC 
TCCATGGAGG ACATCATGGC AGCGTTCGGC GACAACTCCG AGCAGCAGCG AGTGTATCAA 
CAAGCTCAGG AGCAGATACA CAAGATCTTG CATCCTGACG AGCACACACA CTCGTCGTGC 
GGAGAGCCAC GCAAGAGTTC CAGAGGAAGA CGCTCACCCA GACACAGGCA GAGGCAGCAG 
AATTTAAGTG GAAGCGGAAG TCCGAGAGAC AAAGATCGCC TCATTTCTGA TCGAGACGCC 
ACCTACAGGA AGTACCCTGG GGTCAACAAC GTAGCCTATG TGTCTGACGC AGATCAGCTC 
CCAGATGCTG GAAGTCCTTC TCCTACTGAT GAGGTGTTTT TAGACCCTGG ATCGCCTCCG 
TCTGGCCCCG CCCCTTCACC GCCCGCTTAC GTCCCCCCTC AGCCAACCAT TGAAGAAGCC 
CGGCAGCAGA TGCACTCCTT ATTGGACGAT GCTTTTGCTC TGGTCTCGCC CTCTTCTCAA 
GGAAGTCCTG CTCCTCTAAC GTTTACTGGC GTTACGGCGG ACACTGGTGT TGGAGGAATC 
AGCCCCGGTC AACCTTCCAG CCCGTCTCCA CGCCCTGCAC GCCAATGGGG CTCCTCTTCT 
TACCCAGCAG CTCCTGCACA CAGTCCTTTC TCCGCTAGGT ATGCAGAGCT AGGAATGTCT 
CCCACATCGG TCCAGGGTTT ACTGCAGAGG CAGACACAGG TCTCTGGGTA TCTGGTGTCT 
GCAGAGATGC AGTCAGATCC TGGGTATTCC AGCAGGGGAC AATATGGAGA CGAGATGCCC 
TCGTCCTCTC GACCACGACC AGTAGGGGGC AGCACAGGTG CGCAGTTGCA TCAGTTGACA 
CAAGTGGGCT TGTCGAGTCG GATTGGATTG TATCCTGGAG TTGGCCGTAG TGTTTCTGGA 
CCCTCAGGAT CAAGCTGGGC TCAGTACCGC TCAGATGAGG AGATCTCTCG ACCAGGATCC 
AACCGAGAGG CTATAATGAC ATTCCCAGAA TACTCTTCTT CACCAGTATT CCAGATGCCC 
AGGACGTCCC TGCAGGATTC TGCACCCCCC CAGTCCCACC TGGAAGCCTC AAACGCGGGT 
TACATCGCCC CAGAAGAGCA CTCTCCACCA CCTCACTCCT CCGCCTCTCT CATCAAGGCC 
ATCAGGGAAG AACTGCAGAG ACTTTCTCAG AAACAAGTTG CTGTCGTCAG CTACCACAGT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210773.1
CDS	669..5111
Translation	MESSRMVPRHLLGCCVFLLAAVMLVGFTVTLSTHNAAELPSNVSRRSINQLPARTRSPPALSSSSSSAHRSERTRRPAHTPPGTKHPSPSPGHHDSASSIDPTPSLGSEGSGNLGDGAQGIHLLLRPPDLLTPSQMPPLRDDRLPTPAPPTLVPPEDLYPIHSAEMDWGSGDYLETLTFMGSEGEEFSLVTTLPTHGYDPYDFDEDATVSYNTAFPSRPILPLSSRHLQPSATVKYGTHLRTAHPTDPRLPLAPDSDRDDDLDFDWAELFPIEPTEMLLPDMNSLEYYNTLQAKENASIARNKTHPHITPTHTLGPTDSPTGTLGKVPDQVDGITHQPIPSEKPTKPLVPLSTETQRPPKAPEKGVPSIPTSHMGKTKPSVPTAPPPASISEGPVIQAVTLKPPATKPRTTTVKTISRTTTTTTTTTTTTNPTSLSTKSAPPTIPKIHQPIAPRQNVCNISKSEMYLVRVGMPSGSSVEFAKNHVLEILRREFNRSVELKVHKYPPNFIFRAFSGPLVYTAVSVINALRQPMHSTSSSTILSVTTIYAVPDYKHQVHTVLQFVPGHVDVRLCSFSERVEKGLLMAYAEVRKRSQENGNFTVHIVNITSSLPKGQRPQKAPVDITFAVRDSRGFLTGSDVSGHMRQLSAVEFSFYLGFPTLQIAEPFHYPELNVSHLLRASWVKTVLLGVLDQRVNERTFQAKMERRLALLVGEGLGIGINNRRWRRSTSVGNNSVQIVRASRLDGPDYPLELVYFVEEGSGERLPAQATAAMLNRLNLQRAAIVLGYRVHGVLATPVEKLALPPSETQPSNVWLIVGVVVPVLVVVLIIIILYWKLCRSEKLEFQPDAMSTIQQRQKLQPPSVKGFDFAKLHLGQHSKDDIMVIQEPAPLPPSVKEATPSEGGELNTPKSKSSSTKASRAARRRGRLSPSDGDSLGSEASSGRESAEESTRPAVTPSDSKPQHRKSKHAKNKLGAQPGSGVDEQLSSSSIFEHVDRVSRGSSDGTRRVSNKIQLIAMQPMPSPPSHPHNQPLSPNLTDKITDGAKVNSEIQVALRHKSEIEHHRNKIRLRAKRKGHYEFPSMEDIMAAFGDNSEQQRVYQQAQEQIHKILHPDEHTHSSCGEPRKSSRGRRSPRHRQRQQNLSGSGSPRDKDRLISDRDATYRKYPGVNNVAYVSDADQLPDAGSPSPTDEVFLDPGSPPSGPAPSPPAYVPPQPTIEEARQQMHSLLDDAFALVSPSSQGSPAPLTFTGVTADTGVGGISPGQPSSPSPRPARQWGSSSYPAAPAHSPFSARYAELGMSPTSVQGLLQRQTQVSGYLVSAEMQSDPGYSSRGQYGDEMPSSSRPRPVGGSTGAQLHQLTQVGLSSRIGLYPGVGRSVSGPSGSSWAQYRSDEEISRPGSNREAIMTFPEYSSSPVFQMPRTSLQDSAPPQSHLEASNAGYIAPEEHSPPPHSSASLIKAIREELQRLSQKQVAVVSYHS

Target ORF information:

RefSeq Version	XM_017355284.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:dkeyp-27e10.3 (si:dkeyp-27e10.3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017355284.2

ORF Insert Sequence:

ATGGAGAGTT CAAGGATGGT CCCGCGGCAT CTGCTCGGAT GTTGTGTGTT TCTGCTCGCC 
GCTGTGATGC TGGTGGGCTT CACGGTGACA CTCAGCACAC ACAATGCAGC GGAGCTACCT 
TCCAACGTGT CCAGAAGGAG CATCAATCAA CTTCCGGCAA GAACACGCTC ACCTCCTGCC 
TTATCTTCAT CATCCTCATC TGCTCATCGC TCTGAAAGGA CCAGGCGTCC AGCACACACG 
CCTCCAGGTA CCAAACACCC CTCACCATCC CCAGGTCACC ATGACAGTGC CTCCAGCATA 
GATCCCACCC CGTCCCTTGG ATCTGAAGGC TCGGGTAACC TAGGCGATGG GGCCCAAGGC 
ATCCACTTGC TGTTACGTCC ACCAGATCTA CTCACGCCCA GCCAGATGCC ACCTCTGCGG 
GACGACAGAC TCCCTACCCC AGCCCCACCC ACCCTAGTGC CACCCGAAGA CTTATATCCA 
ATCCACTCGG CGGAGATGGA CTGGGGTTCT GGAGACTACC TGGAAACTCT TACATTCATG 
GGATCTGAAG GAGAGGAGTT TTCACTGGTC ACCACATTAC CGACGCATGG ATACGATCCA 
TATGATTTTG ACGAAGATGC CACGGTTAGC TACAACACAG CTTTCCCTTC CCGTCCCATT 
CTTCCTCTTT CATCCAGACA CCTCCAGCCC AGCGCTACCG TAAAATATGG GACTCATTTA 
AGGACCGCCC ATCCTACTGA TCCCCGCCTC CCATTGGCTC CTGATTCTGA TAGAGACGAT 
GATCTGGATT TCGATTGGGC GGAGTTGTTT CCTATTGAGC CAACCGAAAT GCTGCTTCCC 
GACATGAACA GTTTGGAATA CTACAACACG TTACAAGCCA AAGAGAACGC TAGTATTGCG 
AGGAATAAAA CGCATCCTCA TATTACTCCC ACGCACACTT TGGGACCCAC CGACAGTCCT 
ACTGGAACTT TGGGAAAAGT CCCGGATCAG GTTGATGGGA TAACTCATCA ACCAATTCCA 
TCTGAGAAAC CTACAAAACC TCTTGTCCCA TTGAGCACTG AGACCCAGCG ACCTCCAAAA 
GCTCCGGAGA AGGGTGTTCC TTCCATACCT ACATCTCATA TGGGCAAAAC CAAACCATCA 
GTGCCAACAG CGCCCCCTCC AGCCAGCATC TCTGAGGGTC CAGTAATACA AGCCGTCACG 
CTGAAGCCCC CTGCTACAAA ACCCCGTACG ACCACAGTCA AAACCATCTC TAGAACTACA 
ACAACAACAA CAACAACCAC CACTACTACA AATCCCACAA GCCTCTCCAC TAAAAGTGCA 
CCGCCTACAA TTCCCAAAAT TCATCAGCCC ATCGCACCAC GACAGAATGT CTGCAACATC 
AGCAAATCAG AAATGTACCT TGTCCGAGTG GGTATGCCAA GTGGATCATC AGTGGAGTTT 
GCTAAAAATC ATGTTCTTGA GATTCTTCGA CGAGAGTTTA ACCGTTCAGT GGAGCTAAAG 
GTCCACAAAT ATCCACCCAA TTTTATCTTT CGGGCTTTTT CTGGTCCACT GGTCTACACT 
GCTGTGTCAG TCATCAATGC TCTCCGCCAA CCAATGCACA GCACATCGTC TTCTACCATT 
CTCTCTGTTA CAACTATATA TGCAGTGCCT GATTACAAGC ATCAGGTCCA CACAGTGCTG 
CAGTTTGTGC CAGGTCATGT GGATGTGCGT CTGTGTAGCT TTAGCGAGCG AGTGGAAAAG 
GGGCTCCTCA TGGCATATGC GGAAGTGCGC AAACGCTCAC AAGAAAATGG GAATTTCACA 
GTCCATATTG TGAACATAAC TAGTAGCCTT CCCAAGGGTC AGCGTCCGCA GAAGGCTCCA 
GTGGACATCA CGTTTGCTGT GCGTGATTCT CGTGGTTTTC TAACTGGATC GGATGTGAGC 
GGCCACATGA GGCAGCTTAG TGCTGTGGAG TTCAGCTTCT ACCTTGGTTT TCCTACCCTA 
CAGATCGCTG AACCTTTTCA TTATCCTGAA CTCAATGTGT CCCACTTGTT ACGTGCATCC 
TGGGTGAAAA CAGTTTTACT GGGTGTGTTG GATCAGCGTG TGAACGAGCG GACCTTTCAG 
GCCAAAATGG AGCGACGATT AGCATTGCTG GTTGGAGAAG GACTTGGGAT CGGAATTAAC 
AATCGACGAT GGAGAAGATC TACTAGTGTT GGCAACAATA GCGTACAGAT TGTGCGAGCG 
TCTCGACTGG ATGGGCCTGA CTATCCGCTG GAGCTGGTGT ATTTTGTGGA AGAGGGTTCG 
GGAGAGAGAC TGCCAGCCCA GGCCACAGCG GCAATGCTGA ACAGACTGAA CCTGCAGAGA 
GCCGCCATCG TACTGGGATA CCGCGTACAC GGAGTTCTAG CCACACCGGT GGAAAAACTG 
GCCCTGCCGC CGTCTGAGAC TCAGCCCAGT AACGTGTGGC TGATCGTAGG AGTGGTGGTC 
CCAGTGCTGG TGGTGGTCCT CATCATCATC ATCCTCTACT GGAAACTGTG CCGCTCGGAG 
AAGCTGGAGT TTCAGCCAGA CGCCATGAGC ACCATCCAGC AGAGACAGAA GTTGCAGCCT 
CCCAGCGTTA AAGGCTTTGA TTTTGCTAAG CTGCATCTGG GGCAGCATAG TAAGGATGAC 
ATCATGGTAA TTCAGGAACC CGCCCCGTTA CCGCCGTCTG TCAAAGAGGC CACACCCTCA 
GAGGGCGGTG AATTGAACAC GCCTAAATCG AAGAGCTCGT CTACGAAAGC ATCACGTGCA 
GCACGAAGGA GAGGCCGGTT GTCTCCATCT GATGGTGATT CTTTAGGAAG TGAAGCATCT 
AGTGGTAGAG AATCAGCAGA GGAGAGCACC AGACCTGCTG TGACACCCAG TGACAGCAAA 
CCCCAACATC GAAAGAGCAA GCATGCTAAA AATAAACTTG GTGCTCAGCC TGGAAGCGGT 
GTGGATGAGC AGTTGTCCTC ATCGTCCATA TTCGAGCATG TGGACCGTGT GTCTCGAGGC 
TCGTCCGATG GAACACGCAG GGTGTCCAAT AAGATACAGC TTATCGCCAT GCAGCCCATG 
CCGAGCCCAC CCTCACATCC ACATAACCAA CCCCTGAGTC CCAATCTGAC AGACAAGATT 
ACTGACGGAG CAAAGGTCAA CAGTGAGATC CAAGTGGCCT TGAGGCATAA ATCTGAGATC 
GAACACCATC GCAATAAGAT CCGCCTGCGT GCCAAACGAA AGGGCCACTA CGAGTTCCCC 
TCCATGGAGG ACATCATGGC AGCGTTCGGC GACAACTCCG AGCAGCAGCG AGTGTATCAA 
CAAGCTCAGG AGCAGATACA CAAGATCTTG CATCCTGACG AGCACACACA CTCGTCGTGC 
GGAGAGCCAC GCAAGAGTTC CAGAGGAAGA CGCTCACCCA GACACAGGCA GAGGCAGCAG 
AATTTAAGTG GAAGCGGAAG TCCGAGAGAC AAAGATCGCC TCATTTCTGA TCGAGACGCC 
ACCTACAGGA AGTACCCTGG GGTCAACAAC GTAGCCTATG TGTCTGACGC AGATCAGCTC 
CCAGATGCTG GAAGTCCTTC TCCTACTGAT GAGGTGTTTT TAGACCCTGG ATCGCCTCCG 
TCTGGCCCCG CCCCTTCACC GCCCGCTTAC GTCCCCCCTC AGCCAACCAT TGAAGAAGCC 
CGGCAGCAGA TGCACTCCTT ATTGGACGAT GCTTTTGCTC TGGTCTCGCC CTCTTCTCAA 
GGAAGTCCTG CTCCTCTAAC GTTTACTGGC GTTACGGCGG ACACTGGTGT TGGAGGAATC 
AGCCCCGGTC AACCTTCCAG CCCGTCTCCA CGCCCTGCAC GCCAATGGGG CTCCTCTTCT 
TACCCAGCAG CTCCTGCACA CAGTCCTTTC TCCGCTAGGT ATGCAGAGCT AGGAATGTCT 
CCCACATCGG TCCAGGGTTT ACTGCAGAGG CAGACACAGG TCTCTGGGTA TCTGGTGTCT 
GCAGAGATGC AGTCAGATCC TGGGTATTCC AGCAGGGGAC AATATGGAGA CGAGATGCCC 
TCGTCCTCTC GACCACGACC AGTAGGGGGC AGCACAGGTG CGCAGTTGCA TCAGTTGACA 
CAAGTGGGCT TGTCGAGTCG GATTGGATTG TATCCTGGAG TTGGCCGTAG TGTTTCTGGA 
CCCTCAGGAT CAAGCTGGGC TCAGTACCGC TCAGATGAGG AGATCTCTCG ACCAGGATCC 
AACCGAGAGG CTATAATGAC ATTCCCAGAA TACTCTTCTT CACCAGTATT CCAGATGCCC 
AGGACGTCCC TGCAGGATTC TGCACCCCCC CAGTCCCACC TGGAAGCCTC AAACGCGGGT 
TACATCGCCC CAGAAGAGCA CTCTCCACCA CCTCACTCCT CCGCCTCTCT CATCAAGGCC 
ATCAGGGAAG AACTGCAGAG ACTTTCTCAG AAACAAGTTG CTGTCGTCAG CTACCACAGT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:DKEYP-27E10.3 ( XM_021473999.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021329674.1 Danio rerio si:dkeyp-27e10.3 (si:dkeyp-27e10.3), transcript variant X1, mRNA.

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CloneID	ODa146500
Clone ID Related Accession (Same CDS sequence)	XM_021473999.1
Accession Version	XM_021473999.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4506bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	UPF0606 protein KIAA1549 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395230.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGTT CAAGGATGGT CCCGCGGCAT CTGCTCGGAT GTTGTGTGTT TCTGCTCGCC 
GCTGTGATGC TGGTGGGCTT CACGGTGACA CTCAGCACAC ACAATGCAGC GGAGCTACCT 
TCCAACGTGT CCAGAAGGAG CATCAATCAA CTTCCGGCAA GAACACGCTC ACCTCCTGCC 
TTATCTTCAT CATCCTCATC TGCTCATCGC TCTGAAAGGA CCAGGCGTCC AGCACACACG 
CCTCCAGGTA CCAAACACCC CTCACCATCC CCAGGTCACC ATGACAGTGC CTCCAGCATA 
GATCCCACCC CGTCCCTTGG ATCTGAAGGC TCGGGTAACC TAGGCGATGG GGCCCAAGGC 
ATCCACTTGC TGTTACGTCC ACCAGATCTA CTCACGCCCA GCCAGATGCC ACCTCTGCGG 
GACGACAGAC TCCCTACCCC AGCCCCACCC ACCCTAGTGC CACCCGAAGA CTTATATCCA 
ATCCACTCGG CGGAGATGGA CTGGGGTTCT GGAGACTACC TGGAAACTCT TACATTCATG 
GGATCTGAAG GAGAGGAGTT TTCACTGGTC ACCACATTAC CGACGCATGG ATACGATCCA 
TATGATTTTG ACGAAGATGC CACGGTTAGC TACAACACAG CTTTCCCTTC CCGTCCCATT 
CTTCCTCTTT CATCCAGACA CCTCCAGCCC AGCGCTACCG TAAAATATGG GACTCATTTA 
AGGACCGCCC ATCCTACTGA TCCCCGCCTC CCATTGGCTC CTGATTCTGA TAGAGACGAT 
GATCTGGATT TCGATTGGGC GGAGTTGTTT CCTATTGAGC CAACCGAAAT GCTGCTTCCC 
GACATGAACA GTTTGGAATA CTACAACACG TTACAAGCCA AAGAGAACGC TAGTATTGCG 
AGGAATAAAA CGCATCCTCA TATTACTCCC ACGCACACTT TGGGACCCAC CGACAGTCCT 
ACTGGAACTT TGGGAAAAGT CCCGGATCAG GTTGATGGGA TAACTCATCA ACCAATTCCA 
TCTGAGAAAC CTACAAAACC TCTTGTCCCA TTGAGCACTG AGACCCAGCG ACCTCCAAAA 
GCTCCGGAGA AGGGTGTTCC TTCCATACCT ACATCTCATA TGGGCAAAAC CAAACCATCA 
GTGCCAACAG CGCCCCCTCC AGCCAGCATC TCTGAGGGTC CAGTAATACA AGCCGTCACG 
CTGAAGCCCC CTGCTACAAA ACCCCGTACG ACCACAGTCA AAACCATCTC TAGAACTACA 
ACAACAACAA CAACAACCAC CACTACTACA AATCCCACAA GCCTCTCCAC TAAAAGTGCA 
CCGCCTACAA TTCCCAAAAT TCATCAGCCC ATCGCACCAC GACAGAATGT CTGCAACATC 
AGCAAATCAG AAATGTACCT TGTCCGAGTG GGTATGCCAA GTGGATCATC AGTGGAGTTT 
GCTAAAAATC ATGTTCTTGA GATTCTTCGA CGAGAGTTTA ACCGTTCAGT GGAGCTAAAG 
GTCCACAAAT ATCCACCCAA TTTTATCTTT CGGGCTTTTT CTGGTCCACT GGTCTACACT 
GCTGTGTCAG TCATCAATGC TCTCCGCCAA CCAATGCACA GCACATCGTC TTCTACCATT 
CTCTCTGTTA CAACTATATA TGCAGTGCCT GATTACAAGC ATCAGGTCCA CACAGTGCTG 
CAGTTTGTGC CGGGTCATGT CGATGTGCGT CTGTGTAGCT TTAGCGAGCG AGTGGAAAAG 
GGGCTCCTCA TGGCATATGC GGAAGTGCGC AAACGCTCAC AAGAAAATGG GAATTTCACA 
GTCCATATTG TAAACATAAC TAGTAGCCTT CCGAAGGGTC AGCGTCCGCA GAAGGCTCCA 
GTGGACATCA CGTTTGCTGT GCGTGATTCT CGTGGTTTTC TAACTGGATC GGATGTGAGC 
GGCCACATGA GGCAGCTTAG TGCTGTGGAG TTCAGCTTCT ACCTTGGTTT TCCTACCCTT 
CAGATCGCTG AACCTTTTCA TTATCCTGAA CTCAATGTGT CCCACTTGTT ACGTGCATCC 
TGGGTGAAAA CAGTTTTACT GGGTGTGTTG GATCAGCGTG TGAACGAGCG GACCTTTCAG 
GCCAAAATGG AGCGACGATT AGCATTGCTG GTTGGAGAAG GACTTGGGAT CGGAATTAAC 
AATCGACGAT GGAGAAGATC TACTAGTGTT GGCAACAATA GCGTACAGAT TGTGCGAGCG 
TCTCGACTGG ATGGGCCTGA CTATCCGCTG GAGCTGGTGT ATTTTGTGGA AGAGGGTTCG 
GGAGAGAGAC TGCCAGCCCA GGCCACAGCG GCAATGCTGA ACAGACTGAA CCTGCAGAGA 
GCCGCCATCG TACTGGGATA CCGCGTACAT GGAGTTCTAG CCACACCGGT GGAAAAACTG 
GCCCTGCCGC CGTCTGAGAC TCAGCCCAGT AACGTGTGGC TGATCGTAGG AGTGGTGGTC 
CCAGTGCTGG TGGTGGTCCT CATCATCATC ATCCTCTACT GGAAACTGTG CCGCTCGGAG 
AAGCTGGAGT TTCAGCCAGA CGCCATGAGC ACCATCCAGC AGAGACAGAA GGTCTGGGTC 
AATGAGTGTG TGTATGAATG TTTTACCCAC TCACAGACAC CACTTGTGAA GGAGTTGCAG 
CCTCCCAGCG TTAAAGGCTT TGATTTTGCT AAGCTGCATC TGGGGCAGCA TAGTAAGGAT 
GACATCATGG TAATTCAGGA ACCCGCCCCG TTACCGCCGT CTGTCAAAGA GGCCACACCC 
TCAGAGGGCG GTGAATTGAA CACGCCTAAA TCGAAGAGCT CGTCTACGAA AGCATCACGT 
GCAGCACGAA GGAGAGGCCG GTTGTCTCCA TCTGATGGTG ATTCTTTAGG AAGTGAAGCA 
TCCAGTGGTA GAGAATCAGC AGAGGAGAGC ACCAGACCTG CTGTGACACC CAGTGACAGC 
AAACCCCAAC ATCGCAAGAG CAAGCATGCT AAAAATAAAC TTGGTGCTCA GCCTGGAAGC 
GGTGTGGATG AGCAGTTGTC CTCATCGTCC ATATTCGAGC ATGTGGACCG TGTGTCTCGA 
GGCTCGTCAG ATGGAACACG CAGGGTGTCC AATAAGATAC AGCTTATCGC CATGCAGCCC 
ATGCCGAGCC CACCCTCACA TCCACATAAC CAACCCCTGA GTCCCAATCT GACAGACAAG 
ATTACTGACG GAGCACAGGT CAACAGTGAG ATCCAAGTGG CCTTGAGGCA TAAATCTGAG 
ATCGAACACC ATCGCAATAA GATCCGCCTG CGTGCCAAAC GAAAGGGCCA CTACGAGTTC 
CCCTCCATGG AGGACATCAT GGCAGCGTTC GGCGACAACT CCGAGCAGCA GCGAGTGTAT 
CAACAAGCTC AGGAGCAGAT ACACAAGATC TTGCATCCTG ACGAGCACAC ACACTCGTCG 
TGCGGAGAGC CACGCAAGAG TTCCAGAGGA AGACGCTCGC CCAGACACAG GCAGAGGCAG 
CAGAATTTAA GTGGAAGCGG AAGTCCGAGA GACAAAGATC GCCTCATTTC TGATCGAGAC 
GCCACCTACA GGAAGTACCC TGGGGTCAAC AACGTAGCCT ATGTGTCTGA CGCAGATCAG 
CTCCCAGATG CTGGAAGTCC TTCTCCTACT GATGAGGTGT TTTTAGACCC TGGATCGCCT 
CCGTCTGGCC CCGCCCCTTC ACCGCCCGCT TACGTCCCCC CTCAGCCAAC CATTGAAGAA 
GCCCGGCAGC AGATGCACTC CTTATTGGAC GATGCTTTTG CTCTGGTCTC GCCCTCTTCT 
CAAGGAAGTC CTGCTCCTCT AACGTTTACT GGCGTTACGG CGGACACTGG TGTTGGAGGA 
ATCAGCCCCG GTCAACCTTC CAGCCCGTCT CCACGCCCTG CACGCCAATG GGGCTCCTCT 
TCTTACCCAG CAGCTCCTGC ACACAGTCCT TTCTCCGCTA GGTATGCAGA GCTAGGAATG 
TCTCCCACAT CGGTCCAGGG TTTACTGCAG AGGCAGACAC AGGTCTCTGG GTATCTGGTG 
TCTGCAGAGA TGCAGTCAGA TCCTGGGTAC TCCAGCAGGG GACAATATGG AGACGAGATG 
CCCTCGTCCT CTCGACCACG ACCAGTAGGG GGCAGCACAG GTGCGCAGTT GCATCAGTTG 
ACACAAGTGG GCTTGTCGAG TCGGATTGGA TTGTATCCTG GAGTTGGCCG TAGTGTTTCT 
GGACCCTCAG GATCAAGCTG GGCTCAGTAC CGCTCAGATG AGGAGATCTC TCGACCAGGA 
TCCAACCGAG AGGCTATAAT GACATTCCCA GAATACTCTT CTTCACCAGT ATTCCAGATG 
CCCAGGACGT CCCTGCAGGA TTCTGCACCC CCCCAGTCCC ACCTGGAAGC CTCAAACGCG 
GGTTACATCG CCCCAGAAGA GCACTCTCCA CCACCTCACT CCTCCGCCTC TCTCATCAAG 
GCCATCAGGG AAGAACTGCA GAGACTTTCT CAGAAACAAG TTGCTGTCGT CAGCTACCAC 
AGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329674.1
CDS	492..4997
Translation	MESSRMVPRHLLGCCVFLLAAVMLVGFTVTLSTHNAAELPSNVSRRSINQLPARTRSPPALSSSSSSAHRSERTRRPAHTPPGTKHPSPSPGHHDSASSIDPTPSLGSEGSGNLGDGAQGIHLLLRPPDLLTPSQMPPLRDDRLPTPAPPTLVPPEDLYPIHSAEMDWGSGDYLETLTFMGSEGEEFSLVTTLPTHGYDPYDFDEDATVSYNTAFPSRPILPLSSRHLQPSATVKYGTHLRTAHPTDPRLPLAPDSDRDDDLDFDWAELFPIEPTEMLLPDMNSLEYYNTLQAKENASIARNKTHPHITPTHTLGPTDSPTGTLGKVPDQVDGITHQPIPSEKPTKPLVPLSTETQRPPKAPEKGVPSIPTSHMGKTKPSVPTAPPPASISEGPVIQAVTLKPPATKPRTTTVKTISRTTTTTTTTTTTTNPTSLSTKSAPPTIPKIHQPIAPRQNVCNISKSEMYLVRVGMPSGSSVEFAKNHVLEILRREFNRSVELKVHKYPPNFIFRAFSGPLVYTAVSVINALRQPMHSTSSSTILSVTTIYAVPDYKHQVHTVLQFVPGHVDVRLCSFSERVEKGLLMAYAEVRKRSQENGNFTVHIVNITSSLPKGQRPQKAPVDITFAVRDSRGFLTGSDVSGHMRQLSAVEFSFYLGFPTLQIAEPFHYPELNVSHLLRASWVKTVLLGVLDQRVNERTFQAKMERRLALLVGEGLGIGINNRRWRRSTSVGNNSVQIVRASRLDGPDYPLELVYFVEEGSGERLPAQATAAMLNRLNLQRAAIVLGYRVHGVLATPVEKLALPPSETQPSNVWLIVGVVVPVLVVVLIIIILYWKLCRSEKLEFQPDAMSTIQQRQKVWVNECVYECFTHSQTPLVKELQPPSVKGFDFAKLHLGQHSKDDIMVIQEPAPLPPSVKEATPSEGGELNTPKSKSSSTKASRAARRRGRLSPSDGDSLGSEASSGRESAEESTRPAVTPSDSKPQHRKSKHAKNKLGAQPGSGVDEQLSSSSIFEHVDRVSRGSSDGTRRVSNKIQLIAMQPMPSPPSHPHNQPLSPNLTDKITDGAQVNSEIQVALRHKSEIEHHRNKIRLRAKRKGHYEFPSMEDIMAAFGDNSEQQRVYQQAQEQIHKILHPDEHTHSSCGEPRKSSRGRRSPRHRQRQQNLSGSGSPRDKDRLISDRDATYRKYPGVNNVAYVSDADQLPDAGSPSPTDEVFLDPGSPPSGPAPSPPAYVPPQPTIEEARQQMHSLLDDAFALVSPSSQGSPAPLTFTGVTADTGVGGISPGQPSSPSPRPARQWGSSSYPAAPAHSPFSARYAELGMSPTSVQGLLQRQTQVSGYLVSAEMQSDPGYSSRGQYGDEMPSSSRPRPVGGSTGAQLHQLTQVGLSSRIGLYPGVGRSVSGPSGSSWAQYRSDEEISRPGSNREAIMTFPEYSSSPVFQMPRTSLQDSAPPQSHLEASNAGYIAPEEHSPPPHSSASLIKAIREELQRLSQKQVAVVSYHS

Target ORF information:

RefSeq Version	XM_021473999.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:dkeyp-27e10.3 (si:dkeyp-27e10.3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473999.1

ORF Insert Sequence:

ATGGAGAGTT CAAGGATGGT CCCGCGGCAT CTGCTCGGAT GTTGTGTGTT TCTGCTCGCC 
GCTGTGATGC TGGTGGGCTT CACGGTGACA CTCAGCACAC ACAATGCAGC GGAGCTACCT 
TCCAACGTGT CCAGAAGGAG CATCAATCAA CTTCCGGCAA GAACACGCTC ACCTCCTGCC 
TTATCTTCAT CATCCTCATC TGCTCATCGC TCTGAAAGGA CCAGGCGTCC AGCACACACG 
CCTCCAGGTA CCAAACACCC CTCACCATCC CCAGGTCACC ATGACAGTGC CTCCAGCATA 
GATCCCACCC CGTCCCTTGG ATCTGAAGGC TCGGGTAACC TAGGCGATGG GGCCCAAGGC 
ATCCACTTGC TGTTACGTCC ACCAGATCTA CTCACGCCCA GCCAGATGCC ACCTCTGCGG 
GACGACAGAC TCCCTACCCC AGCCCCACCC ACCCTAGTGC CACCCGAAGA CTTATATCCA 
ATCCACTCGG CGGAGATGGA CTGGGGTTCT GGAGACTACC TGGAAACTCT TACATTCATG 
GGATCTGAAG GAGAGGAGTT TTCACTGGTC ACCACATTAC CGACGCATGG ATACGATCCA 
TATGATTTTG ACGAAGATGC CACGGTTAGC TACAACACAG CTTTCCCTTC CCGTCCCATT 
CTTCCTCTTT CATCCAGACA CCTCCAGCCC AGCGCTACCG TAAAATATGG GACTCATTTA 
AGGACCGCCC ATCCTACTGA TCCCCGCCTC CCATTGGCTC CTGATTCTGA TAGAGACGAT 
GATCTGGATT TCGATTGGGC GGAGTTGTTT CCTATTGAGC CAACCGAAAT GCTGCTTCCC 
GACATGAACA GTTTGGAATA CTACAACACG TTACAAGCCA AAGAGAACGC TAGTATTGCG 
AGGAATAAAA CGCATCCTCA TATTACTCCC ACGCACACTT TGGGACCCAC CGACAGTCCT 
ACTGGAACTT TGGGAAAAGT CCCGGATCAG GTTGATGGGA TAACTCATCA ACCAATTCCA 
TCTGAGAAAC CTACAAAACC TCTTGTCCCA TTGAGCACTG AGACCCAGCG ACCTCCAAAA 
GCTCCGGAGA AGGGTGTTCC TTCCATACCT ACATCTCATA TGGGCAAAAC CAAACCATCA 
GTGCCAACAG CGCCCCCTCC AGCCAGCATC TCTGAGGGTC CAGTAATACA AGCCGTCACG 
CTGAAGCCCC CTGCTACAAA ACCCCGTACG ACCACAGTCA AAACCATCTC TAGAACTACA 
ACAACAACAA CAACAACCAC CACTACTACA AATCCCACAA GCCTCTCCAC TAAAAGTGCA 
CCGCCTACAA TTCCCAAAAT TCATCAGCCC ATCGCACCAC GACAGAATGT CTGCAACATC 
AGCAAATCAG AAATGTACCT TGTCCGAGTG GGTATGCCAA GTGGATCATC AGTGGAGTTT 
GCTAAAAATC ATGTTCTTGA GATTCTTCGA CGAGAGTTTA ACCGTTCAGT GGAGCTAAAG 
GTCCACAAAT ATCCACCCAA TTTTATCTTT CGGGCTTTTT CTGGTCCACT GGTCTACACT 
GCTGTGTCAG TCATCAATGC TCTCCGCCAA CCAATGCACA GCACATCGTC TTCTACCATT 
CTCTCTGTTA CAACTATATA TGCAGTGCCT GATTACAAGC ATCAGGTCCA CACAGTGCTG 
CAGTTTGTGC CGGGTCATGT CGATGTGCGT CTGTGTAGCT TTAGCGAGCG AGTGGAAAAG 
GGGCTCCTCA TGGCATATGC GGAAGTGCGC AAACGCTCAC AAGAAAATGG GAATTTCACA 
GTCCATATTG TAAACATAAC TAGTAGCCTT CCGAAGGGTC AGCGTCCGCA GAAGGCTCCA 
GTGGACATCA CGTTTGCTGT GCGTGATTCT CGTGGTTTTC TAACTGGATC GGATGTGAGC 
GGCCACATGA GGCAGCTTAG TGCTGTGGAG TTCAGCTTCT ACCTTGGTTT TCCTACCCTT 
CAGATCGCTG AACCTTTTCA TTATCCTGAA CTCAATGTGT CCCACTTGTT ACGTGCATCC 
TGGGTGAAAA CAGTTTTACT GGGTGTGTTG GATCAGCGTG TGAACGAGCG GACCTTTCAG 
GCCAAAATGG AGCGACGATT AGCATTGCTG GTTGGAGAAG GACTTGGGAT CGGAATTAAC 
AATCGACGAT GGAGAAGATC TACTAGTGTT GGCAACAATA GCGTACAGAT TGTGCGAGCG 
TCTCGACTGG ATGGGCCTGA CTATCCGCTG GAGCTGGTGT ATTTTGTGGA AGAGGGTTCG 
GGAGAGAGAC TGCCAGCCCA GGCCACAGCG GCAATGCTGA ACAGACTGAA CCTGCAGAGA 
GCCGCCATCG TACTGGGATA CCGCGTACAT GGAGTTCTAG CCACACCGGT GGAAAAACTG 
GCCCTGCCGC CGTCTGAGAC TCAGCCCAGT AACGTGTGGC TGATCGTAGG AGTGGTGGTC 
CCAGTGCTGG TGGTGGTCCT CATCATCATC ATCCTCTACT GGAAACTGTG CCGCTCGGAG 
AAGCTGGAGT TTCAGCCAGA CGCCATGAGC ACCATCCAGC AGAGACAGAA GGTCTGGGTC 
AATGAGTGTG TGTATGAATG TTTTACCCAC TCACAGACAC CACTTGTGAA GGAGTTGCAG 
CCTCCCAGCG TTAAAGGCTT TGATTTTGCT AAGCTGCATC TGGGGCAGCA TAGTAAGGAT 
GACATCATGG TAATTCAGGA ACCCGCCCCG TTACCGCCGT CTGTCAAAGA GGCCACACCC 
TCAGAGGGCG GTGAATTGAA CACGCCTAAA TCGAAGAGCT CGTCTACGAA AGCATCACGT 
GCAGCACGAA GGAGAGGCCG GTTGTCTCCA TCTGATGGTG ATTCTTTAGG AAGTGAAGCA 
TCCAGTGGTA GAGAATCAGC AGAGGAGAGC ACCAGACCTG CTGTGACACC CAGTGACAGC 
AAACCCCAAC ATCGCAAGAG CAAGCATGCT AAAAATAAAC TTGGTGCTCA GCCTGGAAGC 
GGTGTGGATG AGCAGTTGTC CTCATCGTCC ATATTCGAGC ATGTGGACCG TGTGTCTCGA 
GGCTCGTCAG ATGGAACACG CAGGGTGTCC AATAAGATAC AGCTTATCGC CATGCAGCCC 
ATGCCGAGCC CACCCTCACA TCCACATAAC CAACCCCTGA GTCCCAATCT GACAGACAAG 
ATTACTGACG GAGCACAGGT CAACAGTGAG ATCCAAGTGG CCTTGAGGCA TAAATCTGAG 
ATCGAACACC ATCGCAATAA GATCCGCCTG CGTGCCAAAC GAAAGGGCCA CTACGAGTTC 
CCCTCCATGG AGGACATCAT GGCAGCGTTC GGCGACAACT CCGAGCAGCA GCGAGTGTAT 
CAACAAGCTC AGGAGCAGAT ACACAAGATC TTGCATCCTG ACGAGCACAC ACACTCGTCG 
TGCGGAGAGC CACGCAAGAG TTCCAGAGGA AGACGCTCGC CCAGACACAG GCAGAGGCAG 
CAGAATTTAA GTGGAAGCGG AAGTCCGAGA GACAAAGATC GCCTCATTTC TGATCGAGAC 
GCCACCTACA GGAAGTACCC TGGGGTCAAC AACGTAGCCT ATGTGTCTGA CGCAGATCAG 
CTCCCAGATG CTGGAAGTCC TTCTCCTACT GATGAGGTGT TTTTAGACCC TGGATCGCCT 
CCGTCTGGCC CCGCCCCTTC ACCGCCCGCT TACGTCCCCC CTCAGCCAAC CATTGAAGAA 
GCCCGGCAGC AGATGCACTC CTTATTGGAC GATGCTTTTG CTCTGGTCTC GCCCTCTTCT 
CAAGGAAGTC CTGCTCCTCT AACGTTTACT GGCGTTACGG CGGACACTGG TGTTGGAGGA 
ATCAGCCCCG GTCAACCTTC CAGCCCGTCT CCACGCCCTG CACGCCAATG GGGCTCCTCT 
TCTTACCCAG CAGCTCCTGC ACACAGTCCT TTCTCCGCTA GGTATGCAGA GCTAGGAATG 
TCTCCCACAT CGGTCCAGGG TTTACTGCAG AGGCAGACAC AGGTCTCTGG GTATCTGGTG 
TCTGCAGAGA TGCAGTCAGA TCCTGGGTAC TCCAGCAGGG GACAATATGG AGACGAGATG 
CCCTCGTCCT CTCGACCACG ACCAGTAGGG GGCAGCACAG GTGCGCAGTT GCATCAGTTG 
ACACAAGTGG GCTTGTCGAG TCGGATTGGA TTGTATCCTG GAGTTGGCCG TAGTGTTTCT 
GGACCCTCAG GATCAAGCTG GGCTCAGTAC CGCTCAGATG AGGAGATCTC TCGACCAGGA 
TCCAACCGAG AGGCTATAAT GACATTCCCA GAATACTCTT CTTCACCAGT ATTCCAGATG 
CCCAGGACGT CCCTGCAGGA TTCTGCACCC CCCCAGTCCC ACCTGGAAGC CTCAAACGCG 
GGTTACATCG CCCCAGAAGA GCACTCTCCA CCACCTCACT CCTCCGCCTC TCTCATCAAG 
GCCATCAGGG AAGAACTGCA GAGACTTTCT CAGAAACAAG TTGCTGTCGT CAGCTACCAC 
AGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:DKEYP-27E10.3 ( XM_017355282.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017210771.1 Danio rerio si:dkeyp-27e10.3 (si:dkeyp-27e10.3), transcript variant X1, mRNA.

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CloneID	ODa48350
Clone ID Related Accession (Same CDS sequence)	XM_017355282.2 , XM_017355282.1
Accession Version	XM_017355282.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4506bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	UPF0606 protein KIAA1549 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017355282.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGTT CAAGGATGGT CCCGCGGCAT CTGCTCGGAT GTTGTGTGTT TCTGCTCGCC 
GCTGTGATGC TGGTGGGCTT CACGGTGACA CTCAGCACAC ACAATGCAGC GGAGCTACCT 
TCCAACGTGT CCAGAAGGAG CATCAATCAA CTTCCGGCAA GAACACGCTC ACCTCCTGCC 
TTATCTTCAT CATCCTCATC TGCTCATCGC TCTGAAAGGA CCAGGCGTCC AGCACACACG 
CCTCCAGGTA CCAAACACCC CTCACCATCC CCAGGTCACC ATGACAGTGC CTCCAGCATA 
GATCCCACCC CGTCCCTTGG ATCTGAAGGC TCGGGTAACC TAGGCGATGG GGCCCAAGGC 
ATCCACTTGC TGTTACGTCC ACCAGATCTA CTCACGCCCA GCCAGATGCC ACCTCTGCGG 
GACGACAGAC TCCCTACCCC AGCCCCACCC ACCCTAGTGC CACCCGAAGA CTTATATCCA 
ATCCACTCGG CGGAGATGGA CTGGGGTTCT GGAGACTACC TGGAAACTCT TACATTCATG 
GGATCTGAAG GAGAGGAGTT TTCACTGGTC ACCACATTAC CGACGCATGG ATACGATCCA 
TATGATTTTG ACGAAGATGC CACGGTTAGC TACAACACAG CTTTCCCTTC CCGTCCCATT 
CTTCCTCTTT CATCCAGACA CCTCCAGCCC AGCGCTACCG TAAAATATGG GACTCATTTA 
AGGACCGCCC ATCCTACTGA TCCCCGCCTC CCATTGGCTC CTGATTCTGA TAGAGACGAT 
GATCTGGATT TCGATTGGGC GGAGTTGTTT CCTATTGAGC CAACCGAAAT GCTGCTTCCC 
GACATGAACA GTTTGGAATA CTACAACACG TTACAAGCCA AAGAGAACGC TAGTATTGCG 
AGGAATAAAA CGCATCCTCA TATTACTCCC ACGCACACTT TGGGACCCAC CGACAGTCCT 
ACTGGAACTT TGGGAAAAGT CCCGGATCAG GTTGATGGGA TAACTCATCA ACCAATTCCA 
TCTGAGAAAC CTACAAAACC TCTTGTCCCA TTGAGCACTG AGACCCAGCG ACCTCCAAAA 
GCTCCGGAGA AGGGTGTTCC TTCCATACCT ACATCTCATA TGGGCAAAAC CAAACCATCA 
GTGCCAACAG CGCCCCCTCC AGCCAGCATC TCTGAGGGTC CAGTAATACA AGCCGTCACG 
CTGAAGCCCC CTGCTACAAA ACCCCGTACG ACCACAGTCA AAACCATCTC TAGAACTACA 
ACAACAACAA CAACAACCAC CACTACTACA AATCCCACAA GCCTCTCCAC TAAAAGTGCA 
CCGCCTACAA TTCCCAAAAT TCATCAGCCC ATCGCACCAC GACAGAATGT CTGCAACATC 
AGCAAATCAG AAATGTACCT TGTCCGAGTG GGTATGCCAA GTGGATCATC AGTGGAGTTT 
GCTAAAAATC ATGTTCTTGA GATTCTTCGA CGAGAGTTTA ACCGTTCAGT GGAGCTAAAG 
GTCCACAAAT ATCCACCCAA TTTTATCTTT CGGGCTTTTT CTGGTCCACT GGTCTACACT 
GCTGTGTCAG TCATCAATGC TCTCCGCCAA CCAATGCACA GCACATCGTC TTCTACCATT 
CTCTCTGTTA CAACTATATA TGCAGTGCCT GATTACAAGC ATCAGGTCCA CACAGTGCTG 
CAGTTTGTGC CAGGTCATGT GGATGTGCGT CTGTGTAGCT TTAGCGAGCG AGTGGAAAAG 
GGGCTCCTCA TGGCATATGC GGAAGTGCGC AAACGCTCAC AAGAAAATGG GAATTTCACA 
GTCCATATTG TGAACATAAC TAGTAGCCTT CCCAAGGGTC AGCGTCCGCA GAAGGCTCCA 
GTGGACATCA CGTTTGCTGT GCGTGATTCT CGTGGTTTTC TAACTGGATC GGATGTGAGC 
GGCCACATGA GGCAGCTTAG TGCTGTGGAG TTCAGCTTCT ACCTTGGTTT TCCTACCCTA 
CAGATCGCTG AACCTTTTCA TTATCCTGAA CTCAATGTGT CCCACTTGTT ACGTGCATCC 
TGGGTGAAAA CAGTTTTACT GGGTGTGTTG GATCAGCGTG TGAACGAGCG GACCTTTCAG 
GCCAAAATGG AGCGACGATT AGCATTGCTG GTTGGAGAAG GACTTGGGAT CGGAATTAAC 
AATCGACGAT GGAGAAGATC TACTAGTGTT GGCAACAATA GCGTACAGAT TGTGCGAGCG 
TCTCGACTGG ATGGGCCTGA CTATCCGCTG GAGCTGGTGT ATTTTGTGGA AGAGGGTTCG 
GGAGAGAGAC TGCCAGCCCA GGCCACAGCG GCAATGCTGA ACAGACTGAA CCTGCAGAGA 
GCCGCCATCG TACTGGGATA CCGCGTACAC GGAGTTCTAG CCACACCGGT GGAAAAACTG 
GCCCTGCCGC CGTCTGAGAC TCAGCCCAGT AACGTGTGGC TGATCGTAGG AGTGGTGGTC 
CCAGTGCTGG TGGTGGTCCT CATCATCATC ATCCTCTACT GGAAACTGTG CCGCTCGGAG 
AAGCTGGAGT TTCAGCCAGA CGCCATGAGC ACCATCCAGC AGAGACAGAA GGTCTGGGTC 
AATGAGTGTG TGTATGAATG TTTTACCCAC TCACAGACAC CACTTGTGAA GGAGTTGCAG 
CCTCCCAGCG TTAAAGGCTT TGATTTTGCT AAGCTGCATC TGGGGCAGCA TAGTAAGGAT 
GACATCATGG TAATTCAGGA ACCCGCCCCG TTACCGCCGT CTGTCAAAGA GGCCACACCC 
TCAGAGGGCG GTGAATTGAA CACGCCTAAA TCGAAGAGCT CGTCTACGAA AGCATCACGT 
GCAGCACGAA GGAGAGGCCG GTTGTCTCCA TCTGATGGTG ATTCTTTAGG AAGTGAAGCA 
TCTAGTGGTA GAGAATCAGC AGAGGAGAGC ACCAGACCTG CTGTGACACC CAGTGACAGC 
AAACCCCAAC ATCGAAAGAG CAAGCATGCT AAAAATAAAC TTGGTGCTCA GCCTGGAAGC 
GGTGTGGATG AGCAGTTGTC CTCATCGTCC ATATTCGAGC ATGTGGACCG TGTGTCTCGA 
GGCTCGTCCG ATGGAACACG CAGGGTGTCC AATAAGATAC AGCTTATCGC CATGCAGCCC 
ATGCCGAGCC CACCCTCACA TCCACATAAC CAACCCCTGA GTCCCAATCT GACAGACAAG 
ATTACTGACG GAGCAAAGGT CAACAGTGAG ATCCAAGTGG CCTTGAGGCA TAAATCTGAG 
ATCGAACACC ATCGCAATAA GATCCGCCTG CGTGCCAAAC GAAAGGGCCA CTACGAGTTC 
CCCTCCATGG AGGACATCAT GGCAGCGTTC GGCGACAACT CCGAGCAGCA GCGAGTGTAT 
CAACAAGCTC AGGAGCAGAT ACACAAGATC TTGCATCCTG ACGAGCACAC ACACTCGTCG 
TGCGGAGAGC CACGCAAGAG TTCCAGAGGA AGACGCTCAC CCAGACACAG GCAGAGGCAG 
CAGAATTTAA GTGGAAGCGG AAGTCCGAGA GACAAAGATC GCCTCATTTC TGATCGAGAC 
GCCACCTACA GGAAGTACCC TGGGGTCAAC AACGTAGCCT ATGTGTCTGA CGCAGATCAG 
CTCCCAGATG CTGGAAGTCC TTCTCCTACT GATGAGGTGT TTTTAGACCC TGGATCGCCT 
CCGTCTGGCC CCGCCCCTTC ACCGCCCGCT TACGTCCCCC CTCAGCCAAC CATTGAAGAA 
GCCCGGCAGC AGATGCACTC CTTATTGGAC GATGCTTTTG CTCTGGTCTC GCCCTCTTCT 
CAAGGAAGTC CTGCTCCTCT AACGTTTACT GGCGTTACGG CGGACACTGG TGTTGGAGGA 
ATCAGCCCCG GTCAACCTTC CAGCCCGTCT CCACGCCCTG CACGCCAATG GGGCTCCTCT 
TCTTACCCAG CAGCTCCTGC ACACAGTCCT TTCTCCGCTA GGTATGCAGA GCTAGGAATG 
TCTCCCACAT CGGTCCAGGG TTTACTGCAG AGGCAGACAC AGGTCTCTGG GTATCTGGTG 
TCTGCAGAGA TGCAGTCAGA TCCTGGGTAT TCCAGCAGGG GACAATATGG AGACGAGATG 
CCCTCGTCCT CTCGACCACG ACCAGTAGGG GGCAGCACAG GTGCGCAGTT GCATCAGTTG 
ACACAAGTGG GCTTGTCGAG TCGGATTGGA TTGTATCCTG GAGTTGGCCG TAGTGTTTCT 
GGACCCTCAG GATCAAGCTG GGCTCAGTAC CGCTCAGATG AGGAGATCTC TCGACCAGGA 
TCCAACCGAG AGGCTATAAT GACATTCCCA GAATACTCTT CTTCACCAGT ATTCCAGATG 
CCCAGGACGT CCCTGCAGGA TTCTGCACCC CCCCAGTCCC ACCTGGAAGC CTCAAACGCG 
GGTTACATCG CCCCAGAAGA GCACTCTCCA CCACCTCACT CCTCCGCCTC TCTCATCAAG 
GCCATCAGGG AAGAACTGCA GAGACTTTCT CAGAAACAAG TTGCTGTCGT CAGCTACCAC 
AGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210771.1
CDS	670..5175
Translation	MESSRMVPRHLLGCCVFLLAAVMLVGFTVTLSTHNAAELPSNVSRRSINQLPARTRSPPALSSSSSSAHRSERTRRPAHTPPGTKHPSPSPGHHDSASSIDPTPSLGSEGSGNLGDGAQGIHLLLRPPDLLTPSQMPPLRDDRLPTPAPPTLVPPEDLYPIHSAEMDWGSGDYLETLTFMGSEGEEFSLVTTLPTHGYDPYDFDEDATVSYNTAFPSRPILPLSSRHLQPSATVKYGTHLRTAHPTDPRLPLAPDSDRDDDLDFDWAELFPIEPTEMLLPDMNSLEYYNTLQAKENASIARNKTHPHITPTHTLGPTDSPTGTLGKVPDQVDGITHQPIPSEKPTKPLVPLSTETQRPPKAPEKGVPSIPTSHMGKTKPSVPTAPPPASISEGPVIQAVTLKPPATKPRTTTVKTISRTTTTTTTTTTTTNPTSLSTKSAPPTIPKIHQPIAPRQNVCNISKSEMYLVRVGMPSGSSVEFAKNHVLEILRREFNRSVELKVHKYPPNFIFRAFSGPLVYTAVSVINALRQPMHSTSSSTILSVTTIYAVPDYKHQVHTVLQFVPGHVDVRLCSFSERVEKGLLMAYAEVRKRSQENGNFTVHIVNITSSLPKGQRPQKAPVDITFAVRDSRGFLTGSDVSGHMRQLSAVEFSFYLGFPTLQIAEPFHYPELNVSHLLRASWVKTVLLGVLDQRVNERTFQAKMERRLALLVGEGLGIGINNRRWRRSTSVGNNSVQIVRASRLDGPDYPLELVYFVEEGSGERLPAQATAAMLNRLNLQRAAIVLGYRVHGVLATPVEKLALPPSETQPSNVWLIVGVVVPVLVVVLIIIILYWKLCRSEKLEFQPDAMSTIQQRQKVWVNECVYECFTHSQTPLVKELQPPSVKGFDFAKLHLGQHSKDDIMVIQEPAPLPPSVKEATPSEGGELNTPKSKSSSTKASRAARRRGRLSPSDGDSLGSEASSGRESAEESTRPAVTPSDSKPQHRKSKHAKNKLGAQPGSGVDEQLSSSSIFEHVDRVSRGSSDGTRRVSNKIQLIAMQPMPSPPSHPHNQPLSPNLTDKITDGAKVNSEIQVALRHKSEIEHHRNKIRLRAKRKGHYEFPSMEDIMAAFGDNSEQQRVYQQAQEQIHKILHPDEHTHSSCGEPRKSSRGRRSPRHRQRQQNLSGSGSPRDKDRLISDRDATYRKYPGVNNVAYVSDADQLPDAGSPSPTDEVFLDPGSPPSGPAPSPPAYVPPQPTIEEARQQMHSLLDDAFALVSPSSQGSPAPLTFTGVTADTGVGGISPGQPSSPSPRPARQWGSSSYPAAPAHSPFSARYAELGMSPTSVQGLLQRQTQVSGYLVSAEMQSDPGYSSRGQYGDEMPSSSRPRPVGGSTGAQLHQLTQVGLSSRIGLYPGVGRSVSGPSGSSWAQYRSDEEISRPGSNREAIMTFPEYSSSPVFQMPRTSLQDSAPPQSHLEASNAGYIAPEEHSPPPHSSASLIKAIREELQRLSQKQVAVVSYHS

Target ORF information:

RefSeq Version	XM_017355282.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:dkeyp-27e10.3 (si:dkeyp-27e10.3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017355282.2

ORF Insert Sequence:

ATGGAGAGTT CAAGGATGGT CCCGCGGCAT CTGCTCGGAT GTTGTGTGTT TCTGCTCGCC 
GCTGTGATGC TGGTGGGCTT CACGGTGACA CTCAGCACAC ACAATGCAGC GGAGCTACCT 
TCCAACGTGT CCAGAAGGAG CATCAATCAA CTTCCGGCAA GAACACGCTC ACCTCCTGCC 
TTATCTTCAT CATCCTCATC TGCTCATCGC TCTGAAAGGA CCAGGCGTCC AGCACACACG 
CCTCCAGGTA CCAAACACCC CTCACCATCC CCAGGTCACC ATGACAGTGC CTCCAGCATA 
GATCCCACCC CGTCCCTTGG ATCTGAAGGC TCGGGTAACC TAGGCGATGG GGCCCAAGGC 
ATCCACTTGC TGTTACGTCC ACCAGATCTA CTCACGCCCA GCCAGATGCC ACCTCTGCGG 
GACGACAGAC TCCCTACCCC AGCCCCACCC ACCCTAGTGC CACCCGAAGA CTTATATCCA 
ATCCACTCGG CGGAGATGGA CTGGGGTTCT GGAGACTACC TGGAAACTCT TACATTCATG 
GGATCTGAAG GAGAGGAGTT TTCACTGGTC ACCACATTAC CGACGCATGG ATACGATCCA 
TATGATTTTG ACGAAGATGC CACGGTTAGC TACAACACAG CTTTCCCTTC CCGTCCCATT 
CTTCCTCTTT CATCCAGACA CCTCCAGCCC AGCGCTACCG TAAAATATGG GACTCATTTA 
AGGACCGCCC ATCCTACTGA TCCCCGCCTC CCATTGGCTC CTGATTCTGA TAGAGACGAT 
GATCTGGATT TCGATTGGGC GGAGTTGTTT CCTATTGAGC CAACCGAAAT GCTGCTTCCC 
GACATGAACA GTTTGGAATA CTACAACACG TTACAAGCCA AAGAGAACGC TAGTATTGCG 
AGGAATAAAA CGCATCCTCA TATTACTCCC ACGCACACTT TGGGACCCAC CGACAGTCCT 
ACTGGAACTT TGGGAAAAGT CCCGGATCAG GTTGATGGGA TAACTCATCA ACCAATTCCA 
TCTGAGAAAC CTACAAAACC TCTTGTCCCA TTGAGCACTG AGACCCAGCG ACCTCCAAAA 
GCTCCGGAGA AGGGTGTTCC TTCCATACCT ACATCTCATA TGGGCAAAAC CAAACCATCA 
GTGCCAACAG CGCCCCCTCC AGCCAGCATC TCTGAGGGTC CAGTAATACA AGCCGTCACG 
CTGAAGCCCC CTGCTACAAA ACCCCGTACG ACCACAGTCA AAACCATCTC TAGAACTACA 
ACAACAACAA CAACAACCAC CACTACTACA AATCCCACAA GCCTCTCCAC TAAAAGTGCA 
CCGCCTACAA TTCCCAAAAT TCATCAGCCC ATCGCACCAC GACAGAATGT CTGCAACATC 
AGCAAATCAG AAATGTACCT TGTCCGAGTG GGTATGCCAA GTGGATCATC AGTGGAGTTT 
GCTAAAAATC ATGTTCTTGA GATTCTTCGA CGAGAGTTTA ACCGTTCAGT GGAGCTAAAG 
GTCCACAAAT ATCCACCCAA TTTTATCTTT CGGGCTTTTT CTGGTCCACT GGTCTACACT 
GCTGTGTCAG TCATCAATGC TCTCCGCCAA CCAATGCACA GCACATCGTC TTCTACCATT 
CTCTCTGTTA CAACTATATA TGCAGTGCCT GATTACAAGC ATCAGGTCCA CACAGTGCTG 
CAGTTTGTGC CAGGTCATGT GGATGTGCGT CTGTGTAGCT TTAGCGAGCG AGTGGAAAAG 
GGGCTCCTCA TGGCATATGC GGAAGTGCGC AAACGCTCAC AAGAAAATGG GAATTTCACA 
GTCCATATTG TGAACATAAC TAGTAGCCTT CCCAAGGGTC AGCGTCCGCA GAAGGCTCCA 
GTGGACATCA CGTTTGCTGT GCGTGATTCT CGTGGTTTTC TAACTGGATC GGATGTGAGC 
GGCCACATGA GGCAGCTTAG TGCTGTGGAG TTCAGCTTCT ACCTTGGTTT TCCTACCCTA 
CAGATCGCTG AACCTTTTCA TTATCCTGAA CTCAATGTGT CCCACTTGTT ACGTGCATCC 
TGGGTGAAAA CAGTTTTACT GGGTGTGTTG GATCAGCGTG TGAACGAGCG GACCTTTCAG 
GCCAAAATGG AGCGACGATT AGCATTGCTG GTTGGAGAAG GACTTGGGAT CGGAATTAAC 
AATCGACGAT GGAGAAGATC TACTAGTGTT GGCAACAATA GCGTACAGAT TGTGCGAGCG 
TCTCGACTGG ATGGGCCTGA CTATCCGCTG GAGCTGGTGT ATTTTGTGGA AGAGGGTTCG 
GGAGAGAGAC TGCCAGCCCA GGCCACAGCG GCAATGCTGA ACAGACTGAA CCTGCAGAGA 
GCCGCCATCG TACTGGGATA CCGCGTACAC GGAGTTCTAG CCACACCGGT GGAAAAACTG 
GCCCTGCCGC CGTCTGAGAC TCAGCCCAGT AACGTGTGGC TGATCGTAGG AGTGGTGGTC 
CCAGTGCTGG TGGTGGTCCT CATCATCATC ATCCTCTACT GGAAACTGTG CCGCTCGGAG 
AAGCTGGAGT TTCAGCCAGA CGCCATGAGC ACCATCCAGC AGAGACAGAA GGTCTGGGTC 
AATGAGTGTG TGTATGAATG TTTTACCCAC TCACAGACAC CACTTGTGAA GGAGTTGCAG 
CCTCCCAGCG TTAAAGGCTT TGATTTTGCT AAGCTGCATC TGGGGCAGCA TAGTAAGGAT 
GACATCATGG TAATTCAGGA ACCCGCCCCG TTACCGCCGT CTGTCAAAGA GGCCACACCC 
TCAGAGGGCG GTGAATTGAA CACGCCTAAA TCGAAGAGCT CGTCTACGAA AGCATCACGT 
GCAGCACGAA GGAGAGGCCG GTTGTCTCCA TCTGATGGTG ATTCTTTAGG AAGTGAAGCA 
TCTAGTGGTA GAGAATCAGC AGAGGAGAGC ACCAGACCTG CTGTGACACC CAGTGACAGC 
AAACCCCAAC ATCGAAAGAG CAAGCATGCT AAAAATAAAC TTGGTGCTCA GCCTGGAAGC 
GGTGTGGATG AGCAGTTGTC CTCATCGTCC ATATTCGAGC ATGTGGACCG TGTGTCTCGA 
GGCTCGTCCG ATGGAACACG CAGGGTGTCC AATAAGATAC AGCTTATCGC CATGCAGCCC 
ATGCCGAGCC CACCCTCACA TCCACATAAC CAACCCCTGA GTCCCAATCT GACAGACAAG 
ATTACTGACG GAGCAAAGGT CAACAGTGAG ATCCAAGTGG CCTTGAGGCA TAAATCTGAG 
ATCGAACACC ATCGCAATAA GATCCGCCTG CGTGCCAAAC GAAAGGGCCA CTACGAGTTC 
CCCTCCATGG AGGACATCAT GGCAGCGTTC GGCGACAACT CCGAGCAGCA GCGAGTGTAT 
CAACAAGCTC AGGAGCAGAT ACACAAGATC TTGCATCCTG ACGAGCACAC ACACTCGTCG 
TGCGGAGAGC CACGCAAGAG TTCCAGAGGA AGACGCTCAC CCAGACACAG GCAGAGGCAG 
CAGAATTTAA GTGGAAGCGG AAGTCCGAGA GACAAAGATC GCCTCATTTC TGATCGAGAC 
GCCACCTACA GGAAGTACCC TGGGGTCAAC AACGTAGCCT ATGTGTCTGA CGCAGATCAG 
CTCCCAGATG CTGGAAGTCC TTCTCCTACT GATGAGGTGT TTTTAGACCC TGGATCGCCT 
CCGTCTGGCC CCGCCCCTTC ACCGCCCGCT TACGTCCCCC CTCAGCCAAC CATTGAAGAA 
GCCCGGCAGC AGATGCACTC CTTATTGGAC GATGCTTTTG CTCTGGTCTC GCCCTCTTCT 
CAAGGAAGTC CTGCTCCTCT AACGTTTACT GGCGTTACGG CGGACACTGG TGTTGGAGGA 
ATCAGCCCCG GTCAACCTTC CAGCCCGTCT CCACGCCCTG CACGCCAATG GGGCTCCTCT 
TCTTACCCAG CAGCTCCTGC ACACAGTCCT TTCTCCGCTA GGTATGCAGA GCTAGGAATG 
TCTCCCACAT CGGTCCAGGG TTTACTGCAG AGGCAGACAC AGGTCTCTGG GTATCTGGTG 
TCTGCAGAGA TGCAGTCAGA TCCTGGGTAT TCCAGCAGGG GACAATATGG AGACGAGATG 
CCCTCGTCCT CTCGACCACG ACCAGTAGGG GGCAGCACAG GTGCGCAGTT GCATCAGTTG 
ACACAAGTGG GCTTGTCGAG TCGGATTGGA TTGTATCCTG GAGTTGGCCG TAGTGTTTCT 
GGACCCTCAG GATCAAGCTG GGCTCAGTAC CGCTCAGATG AGGAGATCTC TCGACCAGGA 
TCCAACCGAG AGGCTATAAT GACATTCCCA GAATACTCTT CTTCACCAGT ATTCCAGATG 
CCCAGGACGT CCCTGCAGGA TTCTGCACCC CCCCAGTCCC ACCTGGAAGC CTCAAACGCG 
GGTTACATCG CCCCAGAAGA GCACTCTCCA CCACCTCACT CCTCCGCCTC TCTCATCAAG 
GCCATCAGGG AAGAACTGCA GAGACTTTCT CAGAAACAAG TTGCTGTCGT CAGCTACCAC 
AGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:DKEYP-27E10.3 ( XM_017355282.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017210771.1 Danio rerio si:dkeyp-27e10.3 (si:dkeyp-27e10.3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa48350
Clone ID Related Accession (Same CDS sequence)	XM_017355282.2 , XM_017355282.1
Accession Version	XM_017355282.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4506bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	UPF0606 protein KIAA1549 homolog isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGTT CAAGGATGGT CCCGCGGCAT CTGCTCGGAT GTTGTGTGTT TCTGCTCGCC 
GCTGTGATGC TGGTGGGCTT CACGGTGACA CTCAGCACAC ACAATGCAGC GGAGCTACCT 
TCCAACGTGT CCAGAAGGAG CATCAATCAA CTTCCGGCAA GAACACGCTC ACCTCCTGCC 
TTATCTTCAT CATCCTCATC TGCTCATCGC TCTGAAAGGA CCAGGCGTCC AGCACACACG 
CCTCCAGGTA CCAAACACCC CTCACCATCC CCAGGTCACC ATGACAGTGC CTCCAGCATA 
GATCCCACCC CGTCCCTTGG ATCTGAAGGC TCGGGTAACC TAGGCGATGG GGCCCAAGGC 
ATCCACTTGC TGTTACGTCC ACCAGATCTA CTCACGCCCA GCCAGATGCC ACCTCTGCGG 
GACGACAGAC TCCCTACCCC AGCCCCACCC ACCCTAGTGC CACCCGAAGA CTTATATCCA 
ATCCACTCGG CGGAGATGGA CTGGGGTTCT GGAGACTACC TGGAAACTCT TACATTCATG 
GGATCTGAAG GAGAGGAGTT TTCACTGGTC ACCACATTAC CGACGCATGG ATACGATCCA 
TATGATTTTG ACGAAGATGC CACGGTTAGC TACAACACAG CTTTCCCTTC CCGTCCCATT 
CTTCCTCTTT CATCCAGACA CCTCCAGCCC AGCGCTACCG TAAAATATGG GACTCATTTA 
AGGACCGCCC ATCCTACTGA TCCCCGCCTC CCATTGGCTC CTGATTCTGA TAGAGACGAT 
GATCTGGATT TCGATTGGGC GGAGTTGTTT CCTATTGAGC CAACCGAAAT GCTGCTTCCC 
GACATGAACA GTTTGGAATA CTACAACACG TTACAAGCCA AAGAGAACGC TAGTATTGCG 
AGGAATAAAA CGCATCCTCA TATTACTCCC ACGCACACTT TGGGACCCAC CGACAGTCCT 
ACTGGAACTT TGGGAAAAGT CCCGGATCAG GTTGATGGGA TAACTCATCA ACCAATTCCA 
TCTGAGAAAC CTACAAAACC TCTTGTCCCA TTGAGCACTG AGACCCAGCG ACCTCCAAAA 
GCTCCGGAGA AGGGTGTTCC TTCCATACCT ACATCTCATA TGGGCAAAAC CAAACCATCA 
GTGCCAACAG CGCCCCCTCC AGCCAGCATC TCTGAGGGTC CAGTAATACA AGCCGTCACG 
CTGAAGCCCC CTGCTACAAA ACCCCGTACG ACCACAGTCA AAACCATCTC TAGAACTACA 
ACAACAACAA CAACAACCAC CACTACTACA AATCCCACAA GCCTCTCCAC TAAAAGTGCA 
CCGCCTACAA TTCCCAAAAT TCATCAGCCC ATCGCACCAC GACAGAATGT CTGCAACATC 
AGCAAATCAG AAATGTACCT TGTCCGAGTG GGTATGCCAA GTGGATCATC AGTGGAGTTT 
GCTAAAAATC ATGTTCTTGA GATTCTTCGA CGAGAGTTTA ACCGTTCAGT GGAGCTAAAG 
GTCCACAAAT ATCCACCCAA TTTTATCTTT CGGGCTTTTT CTGGTCCACT GGTCTACACT 
GCTGTGTCAG TCATCAATGC TCTCCGCCAA CCAATGCACA GCACATCGTC TTCTACCATT 
CTCTCTGTTA CAACTATATA TGCAGTGCCT GATTACAAGC ATCAGGTCCA CACAGTGCTG 
CAGTTTGTGC CAGGTCATGT GGATGTGCGT CTGTGTAGCT TTAGCGAGCG AGTGGAAAAG 
GGGCTCCTCA TGGCATATGC GGAAGTGCGC AAACGCTCAC AAGAAAATGG GAATTTCACA 
GTCCATATTG TGAACATAAC TAGTAGCCTT CCCAAGGGTC AGCGTCCGCA GAAGGCTCCA 
GTGGACATCA CGTTTGCTGT GCGTGATTCT CGTGGTTTTC TAACTGGATC GGATGTGAGC 
GGCCACATGA GGCAGCTTAG TGCTGTGGAG TTCAGCTTCT ACCTTGGTTT TCCTACCCTA 
CAGATCGCTG AACCTTTTCA TTATCCTGAA CTCAATGTGT CCCACTTGTT ACGTGCATCC 
TGGGTGAAAA CAGTTTTACT GGGTGTGTTG GATCAGCGTG TGAACGAGCG GACCTTTCAG 
GCCAAAATGG AGCGACGATT AGCATTGCTG GTTGGAGAAG GACTTGGGAT CGGAATTAAC 
AATCGACGAT GGAGAAGATC TACTAGTGTT GGCAACAATA GCGTACAGAT TGTGCGAGCG 
TCTCGACTGG ATGGGCCTGA CTATCCGCTG GAGCTGGTGT ATTTTGTGGA AGAGGGTTCG 
GGAGAGAGAC TGCCAGCCCA GGCCACAGCG GCAATGCTGA ACAGACTGAA CCTGCAGAGA 
GCCGCCATCG TACTGGGATA CCGCGTACAC GGAGTTCTAG CCACACCGGT GGAAAAACTG 
GCCCTGCCGC CGTCTGAGAC TCAGCCCAGT AACGTGTGGC TGATCGTAGG AGTGGTGGTC 
CCAGTGCTGG TGGTGGTCCT CATCATCATC ATCCTCTACT GGAAACTGTG CCGCTCGGAG 
AAGCTGGAGT TTCAGCCAGA CGCCATGAGC ACCATCCAGC AGAGACAGAA GGTCTGGGTC 
AATGAGTGTG TGTATGAATG TTTTACCCAC TCACAGACAC CACTTGTGAA GGAGTTGCAG 
CCTCCCAGCG TTAAAGGCTT TGATTTTGCT AAGCTGCATC TGGGGCAGCA TAGTAAGGAT 
GACATCATGG TAATTCAGGA ACCCGCCCCG TTACCGCCGT CTGTCAAAGA GGCCACACCC 
TCAGAGGGCG GTGAATTGAA CACGCCTAAA TCGAAGAGCT CGTCTACGAA AGCATCACGT 
GCAGCACGAA GGAGAGGCCG GTTGTCTCCA TCTGATGGTG ATTCTTTAGG AAGTGAAGCA 
TCTAGTGGTA GAGAATCAGC AGAGGAGAGC ACCAGACCTG CTGTGACACC CAGTGACAGC 
AAACCCCAAC ATCGAAAGAG CAAGCATGCT AAAAATAAAC TTGGTGCTCA GCCTGGAAGC 
GGTGTGGATG AGCAGTTGTC CTCATCGTCC ATATTCGAGC ATGTGGACCG TGTGTCTCGA 
GGCTCGTCCG ATGGAACACG CAGGGTGTCC AATAAGATAC AGCTTATCGC CATGCAGCCC 
ATGCCGAGCC CACCCTCACA TCCACATAAC CAACCCCTGA GTCCCAATCT GACAGACAAG 
ATTACTGACG GAGCAAAGGT CAACAGTGAG ATCCAAGTGG CCTTGAGGCA TAAATCTGAG 
ATCGAACACC ATCGCAATAA GATCCGCCTG CGTGCCAAAC GAAAGGGCCA CTACGAGTTC 
CCCTCCATGG AGGACATCAT GGCAGCGTTC GGCGACAACT CCGAGCAGCA GCGAGTGTAT 
CAACAAGCTC AGGAGCAGAT ACACAAGATC TTGCATCCTG ACGAGCACAC ACACTCGTCG 
TGCGGAGAGC CACGCAAGAG TTCCAGAGGA AGACGCTCAC CCAGACACAG GCAGAGGCAG 
CAGAATTTAA GTGGAAGCGG AAGTCCGAGA GACAAAGATC GCCTCATTTC TGATCGAGAC 
GCCACCTACA GGAAGTACCC TGGGGTCAAC AACGTAGCCT ATGTGTCTGA CGCAGATCAG 
CTCCCAGATG CTGGAAGTCC TTCTCCTACT GATGAGGTGT TTTTAGACCC TGGATCGCCT 
CCGTCTGGCC CCGCCCCTTC ACCGCCCGCT TACGTCCCCC CTCAGCCAAC CATTGAAGAA 
GCCCGGCAGC AGATGCACTC CTTATTGGAC GATGCTTTTG CTCTGGTCTC GCCCTCTTCT 
CAAGGAAGTC CTGCTCCTCT AACGTTTACT GGCGTTACGG CGGACACTGG TGTTGGAGGA 
ATCAGCCCCG GTCAACCTTC CAGCCCGTCT CCACGCCCTG CACGCCAATG GGGCTCCTCT 
TCTTACCCAG CAGCTCCTGC ACACAGTCCT TTCTCCGCTA GGTATGCAGA GCTAGGAATG 
TCTCCCACAT CGGTCCAGGG TTTACTGCAG AGGCAGACAC AGGTCTCTGG GTATCTGGTG 
TCTGCAGAGA TGCAGTCAGA TCCTGGGTAT TCCAGCAGGG GACAATATGG AGACGAGATG 
CCCTCGTCCT CTCGACCACG ACCAGTAGGG GGCAGCACAG GTGCGCAGTT GCATCAGTTG 
ACACAAGTGG GCTTGTCGAG TCGGATTGGA TTGTATCCTG GAGTTGGCCG TAGTGTTTCT 
GGACCCTCAG GATCAAGCTG GGCTCAGTAC CGCTCAGATG AGGAGATCTC TCGACCAGGA 
TCCAACCGAG AGGCTATAAT GACATTCCCA GAATACTCTT CTTCACCAGT ATTCCAGATG 
CCCAGGACGT CCCTGCAGGA TTCTGCACCC CCCCAGTCCC ACCTGGAAGC CTCAAACGCG 
GGTTACATCG CCCCAGAAGA GCACTCTCCA CCACCTCACT CCTCCGCCTC TCTCATCAAG 
GCCATCAGGG AAGAACTGCA GAGACTTTCT CAGAAACAAG TTGCTGTCGT CAGCTACCAC 
AGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210771.1
CDS	930..5435
Translation	MESSRMVPRHLLGCCVFLLAAVMLVGFTVTLSTHNAAELPSNVSRRSINQLPARTRSPPALSSSSSSAHRSERTRRPAHTPPGTKHPSPSPGHHDSASSIDPTPSLGSEGSGNLGDGAQGIHLLLRPPDLLTPSQMPPLRDDRLPTPAPPTLVPPEDLYPIHSAEMDWGSGDYLETLTFMGSEGEEFSLVTTLPTHGYDPYDFDEDATVSYNTAFPSRPILPLSSRHLQPSATVKYGTHLRTAHPTDPRLPLAPDSDRDDDLDFDWAELFPIEPTEMLLPDMNSLEYYNTLQAKENASIARNKTHPHITPTHTLGPTDSPTGTLGKVPDQVDGITHQPIPSEKPTKPLVPLSTETQRPPKAPEKGVPSIPTSHMGKTKPSVPTAPPPASISEGPVIQAVTLKPPATKPRTTTVKTISRTTTTTTTTTTTTNPTSLSTKSAPPTIPKIHQPIAPRQNVCNISKSEMYLVRVGMPSGSSVEFAKNHVLEILRREFNRSVELKVHKYPPNFIFRAFSGPLVYTAVSVINALRQPMHSTSSSTILSVTTIYAVPDYKHQVHTVLQFVPGHVDVRLCSFSERVEKGLLMAYAEVRKRSQENGNFTVHIVNITSSLPKGQRPQKAPVDITFAVRDSRGFLTGSDVSGHMRQLSAVEFSFYLGFPTLQIAEPFHYPELNVSHLLRASWVKTVLLGVLDQRVNERTFQAKMERRLALLVGEGLGIGINNRRWRRSTSVGNNSVQIVRASRLDGPDYPLELVYFVEEGSGERLPAQATAAMLNRLNLQRAAIVLGYRVHGVLATPVEKLALPPSETQPSNVWLIVGVVVPVLVVVLIIIILYWKLCRSEKLEFQPDAMSTIQQRQKVWVNECVYECFTHSQTPLVKELQPPSVKGFDFAKLHLGQHSKDDIMVIQEPAPLPPSVKEATPSEGGELNTPKSKSSSTKASRAARRRGRLSPSDGDSLGSEASSGRESAEESTRPAVTPSDSKPQHRKSKHAKNKLGAQPGSGVDEQLSSSSIFEHVDRVSRGSSDGTRRVSNKIQLIAMQPMPSPPSHPHNQPLSPNLTDKITDGAKVNSEIQVALRHKSEIEHHRNKIRLRAKRKGHYEFPSMEDIMAAFGDNSEQQRVYQQAQEQIHKILHPDEHTHSSCGEPRKSSRGRRSPRHRQRQQNLSGSGSPRDKDRLISDRDATYRKYPGVNNVAYVSDADQLPDAGSPSPTDEVFLDPGSPPSGPAPSPPAYVPPQPTIEEARQQMHSLLDDAFALVSPSSQGSPAPLTFTGVTADTGVGGISPGQPSSPSPRPARQWGSSSYPAAPAHSPFSARYAELGMSPTSVQGLLQRQTQVSGYLVSAEMQSDPGYSSRGQYGDEMPSSSRPRPVGGSTGAQLHQLTQVGLSSRIGLYPGVGRSVSGPSGSSWAQYRSDEEISRPGSNREAIMTFPEYSSSPVFQMPRTSLQDSAPPQSHLEASNAGYIAPEEHSPPPHSSASLIKAIREELQRLSQKQVAVVSYHS

Target ORF information:

RefSeq Version	XM_017355282.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:dkeyp-27e10.3 (si:dkeyp-27e10.3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017355282.1

ORF Insert Sequence:

ATGGAGAGTT CAAGGATGGT CCCGCGGCAT CTGCTCGGAT GTTGTGTGTT TCTGCTCGCC 
GCTGTGATGC TGGTGGGCTT CACGGTGACA CTCAGCACAC ACAATGCAGC GGAGCTACCT 
TCCAACGTGT CCAGAAGGAG CATCAATCAA CTTCCGGCAA GAACACGCTC ACCTCCTGCC 
TTATCTTCAT CATCCTCATC TGCTCATCGC TCTGAAAGGA CCAGGCGTCC AGCACACACG 
CCTCCAGGTA CCAAACACCC CTCACCATCC CCAGGTCACC ATGACAGTGC CTCCAGCATA 
GATCCCACCC CGTCCCTTGG ATCTGAAGGC TCGGGTAACC TAGGCGATGG GGCCCAAGGC 
ATCCACTTGC TGTTACGTCC ACCAGATCTA CTCACGCCCA GCCAGATGCC ACCTCTGCGG 
GACGACAGAC TCCCTACCCC AGCCCCACCC ACCCTAGTGC CACCCGAAGA CTTATATCCA 
ATCCACTCGG CGGAGATGGA CTGGGGTTCT GGAGACTACC TGGAAACTCT TACATTCATG 
GGATCTGAAG GAGAGGAGTT TTCACTGGTC ACCACATTAC CGACGCATGG ATACGATCCA 
TATGATTTTG ACGAAGATGC CACGGTTAGC TACAACACAG CTTTCCCTTC CCGTCCCATT 
CTTCCTCTTT CATCCAGACA CCTCCAGCCC AGCGCTACCG TAAAATATGG GACTCATTTA 
AGGACCGCCC ATCCTACTGA TCCCCGCCTC CCATTGGCTC CTGATTCTGA TAGAGACGAT 
GATCTGGATT TCGATTGGGC GGAGTTGTTT CCTATTGAGC CAACCGAAAT GCTGCTTCCC 
GACATGAACA GTTTGGAATA CTACAACACG TTACAAGCCA AAGAGAACGC TAGTATTGCG 
AGGAATAAAA CGCATCCTCA TATTACTCCC ACGCACACTT TGGGACCCAC CGACAGTCCT 
ACTGGAACTT TGGGAAAAGT CCCGGATCAG GTTGATGGGA TAACTCATCA ACCAATTCCA 
TCTGAGAAAC CTACAAAACC TCTTGTCCCA TTGAGCACTG AGACCCAGCG ACCTCCAAAA 
GCTCCGGAGA AGGGTGTTCC TTCCATACCT ACATCTCATA TGGGCAAAAC CAAACCATCA 
GTGCCAACAG CGCCCCCTCC AGCCAGCATC TCTGAGGGTC CAGTAATACA AGCCGTCACG 
CTGAAGCCCC CTGCTACAAA ACCCCGTACG ACCACAGTCA AAACCATCTC TAGAACTACA 
ACAACAACAA CAACAACCAC CACTACTACA AATCCCACAA GCCTCTCCAC TAAAAGTGCA 
CCGCCTACAA TTCCCAAAAT TCATCAGCCC ATCGCACCAC GACAGAATGT CTGCAACATC 
AGCAAATCAG AAATGTACCT TGTCCGAGTG GGTATGCCAA GTGGATCATC AGTGGAGTTT 
GCTAAAAATC ATGTTCTTGA GATTCTTCGA CGAGAGTTTA ACCGTTCAGT GGAGCTAAAG 
GTCCACAAAT ATCCACCCAA TTTTATCTTT CGGGCTTTTT CTGGTCCACT GGTCTACACT 
GCTGTGTCAG TCATCAATGC TCTCCGCCAA CCAATGCACA GCACATCGTC TTCTACCATT 
CTCTCTGTTA CAACTATATA TGCAGTGCCT GATTACAAGC ATCAGGTCCA CACAGTGCTG 
CAGTTTGTGC CAGGTCATGT GGATGTGCGT CTGTGTAGCT TTAGCGAGCG AGTGGAAAAG 
GGGCTCCTCA TGGCATATGC GGAAGTGCGC AAACGCTCAC AAGAAAATGG GAATTTCACA 
GTCCATATTG TGAACATAAC TAGTAGCCTT CCCAAGGGTC AGCGTCCGCA GAAGGCTCCA 
GTGGACATCA CGTTTGCTGT GCGTGATTCT CGTGGTTTTC TAACTGGATC GGATGTGAGC 
GGCCACATGA GGCAGCTTAG TGCTGTGGAG TTCAGCTTCT ACCTTGGTTT TCCTACCCTA 
CAGATCGCTG AACCTTTTCA TTATCCTGAA CTCAATGTGT CCCACTTGTT ACGTGCATCC 
TGGGTGAAAA CAGTTTTACT GGGTGTGTTG GATCAGCGTG TGAACGAGCG GACCTTTCAG 
GCCAAAATGG AGCGACGATT AGCATTGCTG GTTGGAGAAG GACTTGGGAT CGGAATTAAC 
AATCGACGAT GGAGAAGATC TACTAGTGTT GGCAACAATA GCGTACAGAT TGTGCGAGCG 
TCTCGACTGG ATGGGCCTGA CTATCCGCTG GAGCTGGTGT ATTTTGTGGA AGAGGGTTCG 
GGAGAGAGAC TGCCAGCCCA GGCCACAGCG GCAATGCTGA ACAGACTGAA CCTGCAGAGA 
GCCGCCATCG TACTGGGATA CCGCGTACAC GGAGTTCTAG CCACACCGGT GGAAAAACTG 
GCCCTGCCGC CGTCTGAGAC TCAGCCCAGT AACGTGTGGC TGATCGTAGG AGTGGTGGTC 
CCAGTGCTGG TGGTGGTCCT CATCATCATC ATCCTCTACT GGAAACTGTG CCGCTCGGAG 
AAGCTGGAGT TTCAGCCAGA CGCCATGAGC ACCATCCAGC AGAGACAGAA GGTCTGGGTC 
AATGAGTGTG TGTATGAATG TTTTACCCAC TCACAGACAC CACTTGTGAA GGAGTTGCAG 
CCTCCCAGCG TTAAAGGCTT TGATTTTGCT AAGCTGCATC TGGGGCAGCA TAGTAAGGAT 
GACATCATGG TAATTCAGGA ACCCGCCCCG TTACCGCCGT CTGTCAAAGA GGCCACACCC 
TCAGAGGGCG GTGAATTGAA CACGCCTAAA TCGAAGAGCT CGTCTACGAA AGCATCACGT 
GCAGCACGAA GGAGAGGCCG GTTGTCTCCA TCTGATGGTG ATTCTTTAGG AAGTGAAGCA 
TCTAGTGGTA GAGAATCAGC AGAGGAGAGC ACCAGACCTG CTGTGACACC CAGTGACAGC 
AAACCCCAAC ATCGAAAGAG CAAGCATGCT AAAAATAAAC TTGGTGCTCA GCCTGGAAGC 
GGTGTGGATG AGCAGTTGTC CTCATCGTCC ATATTCGAGC ATGTGGACCG TGTGTCTCGA 
GGCTCGTCCG ATGGAACACG CAGGGTGTCC AATAAGATAC AGCTTATCGC CATGCAGCCC 
ATGCCGAGCC CACCCTCACA TCCACATAAC CAACCCCTGA GTCCCAATCT GACAGACAAG 
ATTACTGACG GAGCAAAGGT CAACAGTGAG ATCCAAGTGG CCTTGAGGCA TAAATCTGAG 
ATCGAACACC ATCGCAATAA GATCCGCCTG CGTGCCAAAC GAAAGGGCCA CTACGAGTTC 
CCCTCCATGG AGGACATCAT GGCAGCGTTC GGCGACAACT CCGAGCAGCA GCGAGTGTAT 
CAACAAGCTC AGGAGCAGAT ACACAAGATC TTGCATCCTG ACGAGCACAC ACACTCGTCG 
TGCGGAGAGC CACGCAAGAG TTCCAGAGGA AGACGCTCAC CCAGACACAG GCAGAGGCAG 
CAGAATTTAA GTGGAAGCGG AAGTCCGAGA GACAAAGATC GCCTCATTTC TGATCGAGAC 
GCCACCTACA GGAAGTACCC TGGGGTCAAC AACGTAGCCT ATGTGTCTGA CGCAGATCAG 
CTCCCAGATG CTGGAAGTCC TTCTCCTACT GATGAGGTGT TTTTAGACCC TGGATCGCCT 
CCGTCTGGCC CCGCCCCTTC ACCGCCCGCT TACGTCCCCC CTCAGCCAAC CATTGAAGAA 
GCCCGGCAGC AGATGCACTC CTTATTGGAC GATGCTTTTG CTCTGGTCTC GCCCTCTTCT 
CAAGGAAGTC CTGCTCCTCT AACGTTTACT GGCGTTACGG CGGACACTGG TGTTGGAGGA 
ATCAGCCCCG GTCAACCTTC CAGCCCGTCT CCACGCCCTG CACGCCAATG GGGCTCCTCT 
TCTTACCCAG CAGCTCCTGC ACACAGTCCT TTCTCCGCTA GGTATGCAGA GCTAGGAATG 
TCTCCCACAT CGGTCCAGGG TTTACTGCAG AGGCAGACAC AGGTCTCTGG GTATCTGGTG 
TCTGCAGAGA TGCAGTCAGA TCCTGGGTAT TCCAGCAGGG GACAATATGG AGACGAGATG 
CCCTCGTCCT CTCGACCACG ACCAGTAGGG GGCAGCACAG GTGCGCAGTT GCATCAGTTG 
ACACAAGTGG GCTTGTCGAG TCGGATTGGA TTGTATCCTG GAGTTGGCCG TAGTGTTTCT 
GGACCCTCAG GATCAAGCTG GGCTCAGTAC CGCTCAGATG AGGAGATCTC TCGACCAGGA 
TCCAACCGAG AGGCTATAAT GACATTCCCA GAATACTCTT CTTCACCAGT ATTCCAGATG 
CCCAGGACGT CCCTGCAGGA TTCTGCACCC CCCCAGTCCC ACCTGGAAGC CTCAAACGCG 
GGTTACATCG CCCCAGAAGA GCACTCTCCA CCACCTCACT CCTCCGCCTC TCTCATCAAG 
GCCATCAGGG AAGAACTGCA GAGACTTTCT CAGAAACAAG TTGCTGTCGT CAGCTACCAC 
AGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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SI:DKEYP-27E10.3 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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