eif2ak2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	eif2ak2
Entrez Gene ID	100001092
Full Name	eukaryotic translation initiation factor 2-alpha kinase 2
Synonyms	pkr
General protein information	Preferred Names eukaryotic translation initiation factor 2-alpha kinase 2 Names interferon-induced, double-stranded RNA-activated protein kinase IFN-stimulated dsRNA-activated eIF2-alpha kinase 2 double-stranded RNA activated protein kinase
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017358660.1	XP_017214149.1	interferon-induced, double-stranded RNA-activated protein kinase isoform X1
NM_001114470.1	NP_001107942.1	interferon-induced, double-stranded RNA-activated protein kinase

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	EIF2AK2	NP_001077417.1
Canis lupus familiaris (dog)	EIF2AK2	NP_001041600.1
Homo sapiens (human)	EIF2AK2	NP_002750.1
Pan troglodytes (chimpanzee)	EIF2AK2	NP_001138509.1
Gallus gallus (chicken)	EIF2AK2	NP_989818.1
Xenopus tropicalis (tropical clawed frog)	pkr3	XP_002933926.1
Bos taurus (cattle)	EIF2AK2	NP_835210.2
Rattus norvegicus (Norway rat)	Eif2ak2	NP_062208.1
Xenopus tropicalis (tropical clawed frog)	eif2ak2	NP_001072537.1
Mus musculus (house mouse)	Eif2ak2	NP_035293.1
Danio rerio (zebrafish)	eif2ak2	NP_001107942.1

Gene Ontology
Bibliography

Related articles in PubMed

RNF135 is a positive regulator of IFN expression and involved in RIG-I signaling pathway by targeting RIG-I.
Lai Y, Liang M, Hu, Zeng Z, Lin H, Yi G, Li M, Liu Z
Fish & shellfish immunology86474-479(2019 Mar)

NOD1 deficiency impairs CD44a/Lck as well as PI3K/Akt pathway.
Hu YW, Wu XM, Ren SS, Cao L, Nie P, Chang MX
Scientific reports7(1)2979(2017 06)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Contrasted innate responses to two viruses in zebrafish: insights into the ancestral repertoire of vertebrate IFN-stimulated genes.
Briolat V, Jouneau L, Carvalho R, Palha N, Langevin C, Herbomel P, Schwartz O, Spaink HP, Levraud JP, Boudinot P
Journal of immunology (Baltimore, Md. : 1950)192(9)4328-41(2014 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following eif2ak2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the eif2ak2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa36651	NM_001114470.1 Latest version!	Danio rerio eukaryotic translation initiation factor 2-alpha kinase 2 (eif2ak2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ODa54641	XM_017358660.1 Latest version!	Danio rerio eukaryotic translation initiation factor 2-alpha kinase 2 (eif2ak2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

eif2ak2 ( NM_001114470.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001107942.1 Danio rerio eukaryotic translation initiation factor 2-alpha kinase 2 (eif2ak2), mRNA.

Price & Availability↑

CloneID	ODa36651
Clone ID Related Accession (Same CDS sequence)	NM_001114470.1
Accession Version	NM_001114470.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1563552000000
Organism	Danio rerio(zebrafish)
Product	interferon-induced, double-stranded RNA-activated protein kinase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EU289209.1. On Feb 10, 2008 this sequence version replaced XM_001341126.1. ##Evidence-Data-START## Transcript exon combination :: EU289209.1, AM421526.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGAGTCTC TGTCAGGAAA TTATACTTCT CTGCTGAATG AGTATCAGCA GAAAACCCAA 
TGCACTGTTG AGTTTGAGGA GGGACCCACT GATGGACCAA GCCACAATAA AAGGTTTACT 
ATGAGAGCTA TTGTGAATGG ACAGAAGTTT CCTGATGGTA CTGGTAAAAC CAAGAAGGAG 
GCAAAGCAAA ATGCTGCTAA AAACGCACTG GAAGGCTTGA AGAGCACTCA CAGTGATGAA 
CCGACACCTT CACCCGTTGA AAACATTTCA GTTAGTAAAA TAGCATCTCA TCCGAATTAC 
ACCTGCTGGC TCAATGAGCA TTCGCAGAAG AGCAGGTTAA TGTTCAAGGC CTGCGAGTCA 
ACTAAGATGG ATCCAGGAAA CCTCACTCGG TTATGCACGT ATGTGTGTAA GTATGTTTGC 
GATGATAAAG AGTTTCCTGA AGGTTATGGG AAAAATAAGA AGGAAGCCAA AGAGGCAGCG 
GCTCTTCGAG TTTATGAAGA GCTCAACAAA ACACCAAATA CAGAGGTCTT GGATGAGAAC 
AGCAATAGAG CTCAGAGGTC AGAAACTACA TCTTATAGCC TGGACCGCCG TTCGAGTGTT 
GCAGATGATT CTCGAAGCAG CACGCCTGAC AACAACTACA TCGCATATCT CAATAACTAC 
TGCCAGAAGA AAAAACGTGT ATATGACTTC AAATTAGTGG ACAGAATAGG TCCTCCTCAC 
AACCCAATAT TTGTTTATAA AGTTGTGATG GATGGGAAGG AATACCCTGA AGCACAGGGA 
AGAAACGCTA AAGAAGCGAA ACAAAATGCA GCTCAACACG CTTGGAGTGA AATTCGGGAT 
CAGTCTGGCT GGACCACACC GAGTTCTGAA GATGGCGCAT TCTCACAATC CCAAGATACA 
TGTAAATCTG AAGACGTCCA GCGATCAGCG AACGCCTCCA GCACAAGTAA TTTCATCTCC 
TTCAGAGACT CTGCTGCTGC TGCCAGTTCT CCAATGGCCA CCAGTCCTTC TGTGAGCCTA 
GTGGATGTGA AGCCAAAAAT AAAATTAGCA CCAAGATTTC TGTTACCAAA CGGTCTGGAC 
AGAAGTAAAG AGGGTGGTCC TGACATGAAG GTACCAAACC CAGCAAAGGC ATCTGGAGGT 
CAAACTCCAA ACAGTGCTAC TAAATCAAGG TTTTTAGAAG ACTTTGACTC AATAAATCCG 
ATCGGGAAAG GTGGCTTTGG TCGAGTTTTC AAGGCAAGAC GAAAGCTTGA AGACAAATAT 
TATGCCGTGA AGATAGTGAA AAGTACAGAG AAAGCTCGTC GTGAGGTTGG TGCTTTGGCT 
GATTTTAATA ATCCCAACAT CGTCCGCTAC TTCTCGTCTT GGGAAGAGGA CACAGCATAC 
AAACACGAGT CGTCAGAGAG CTTCTCAGAC TCTGGCAGCG GCCCGGGAAC AAAGTTTCTC 
TACATTCAAA TGGAGTTTTG TGAAGGAAAC ACACTCCGAG TCTGGATTAA AGAAAGAAAC 
TCCTCAAACA AACAGTCCCC GGAGCGAAGA ACAGAAGCAG CTCAGATCTA CCGGCAGGTG 
CTGAAGGCTG TGGAGTACAT TCACTCTAAA TTATTGATCC ACAGAGACCT GAAGCCTCCA 
AACATAATGT TCAGCAGTGA GGGAAGAGTA AAGGTTGGAG ACTTTGGCCT CGTGACTGCG 
GCAGAAAATG AAAATGAGGA GCAACTGCTG GAACGAACTA AAAGGACGGG AACACGTACC 
TACATGAGCC CAGAGCAGAT GAATCAAACA ACATATGACA GAAAGGTGGA CATCTACGCT 
CTGGGTCTCA TCTATTTTGA ACTTGTCTGG AATCTTGGCA CAATGCATGA AAAGGAAAAG 
ATATGGGATA AGTTAAGAGT GAGGGTTTTT CCAGTGCAGT TCACAAAGAT GTTTATCTTT 
GAGCACAAGT TGATGGAGCG AATGCTGAGT CCCAGACCTG AAGACAGACC AGATGCCACA 
GACCTGATCC TGGATCTGGA TCAACATTAC ACACTCCTGA AGACGGATCA AGACCTCAGG 
ACCGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001107942.1
CDS	1..2049
Translation	MESLSGNYTSLLNEYQQKTQCTVEFEEGPTDGPSHNKRFTMRAIVNGQKFPDGTGKTKKEAKQNAAKNALEGLKSTHSDEPTPSPVENISVSKIASHPNYTCWLNEHSQKSRLMFKACESTKMDPGNLTRLCTYVCKYVCDDKEFPEGYGKNKKEAKEAAALRVYEELNKTPNTEVLDENSNRAQRSETTSYSLDRRSSVADDSRSSTPDNNYIAYLNNYCQKKKRVYDFKLVDRIGPPHNPIFVYKVVMDGKEYPEAQGRNAKEAKQNAAQHAWSEIRDQSGWTTPSSEDGAFSQSQDTCKSEDVQRSANASSTSNFISFRDSAAAASSPMATSPSVSLVDVKPKIKLAPRFLLPNGLDRSKEGGPDMKVPNPAKASGGQTPNSATKSRFLEDFDSINPIGKGGFGRVFKARRKLEDKYYAVKIVKSTEKARREVGALADFNNPNIVRYFSSWEEDTAYKHESSESFSDSGSGPGTKFLYIQMEFCEGNTLRVWIKERNSSNKQSPERRTEAAQIYRQVLKAVEYIHSKLLIHRDLKPPNIMFSSEGRVKVGDFGLVTAAENENEEQLLERTKRTGTRTYMSPEQMNQTTYDRKVDIYALGLIYFELVWNLGTMHEKEKIWDKLRVRVFPVQFTKMFIFEHKLMERMLSPRPEDRPDATDLILDLDQHYTLLKTDQDLRTV

Target ORF information:

RefSeq Version	NM_001114470.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio eukaryotic translation initiation factor 2-alpha kinase 2 (eif2ak2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001114470.1

ORF Insert Sequence:

ATGGAGTCTC TGTCAGGAAA TTATACTTCT CTGCTGAATG AGTATCAGCA GAAAACCCAA 
TGCACTGTTG AGTTTGAGGA GGGACCCACT GATGGACCAA GCCACAATAA AAGGTTTACT 
ATGAGAGCTA TTGTGAATGG ACAGAAGTTT CCTGATGGTA CTGGTAAAAC CAAGAAGGAG 
GCAAAGCAAA ATGCTGCTAA AAACGCACTG GAAGGCTTGA AGAGCACTCA CAGTGATGAA 
CCGACACCTT CACCCGTTGA AAACATTTCA GTTAGTAAAA TAGCATCTCA TCCGAATTAC 
ACCTGCTGGC TCAATGAGCA TTCGCAGAAG AGCAGGTTAA TGTTCAAGGC CTGCGAGTCA 
ACTAAGATGG ATCCAGGAAA CCTCACTCGG TTATGCACGT ATGTGTGTAA GTATGTTTGC 
GATGATAAAG AGTTTCCTGA AGGTTATGGG AAAAATAAGA AGGAAGCCAA AGAGGCAGCG 
GCTCTTCGAG TTTATGAAGA GCTCAACAAA ACACCAAATA CAGAGGTCTT GGATGAGAAC 
AGCAATAGAG CTCAGAGGTC AGAAACTACA TCTTATAGCC TGGACCGCCG TTCGAGTGTT 
GCAGATGATT CTCGAAGCAG CACGCCTGAC AACAACTACA TCGCATATCT CAATAACTAC 
TGCCAGAAGA AAAAACGTGT ATATGACTTC AAATTAGTGG ACAGAATAGG TCCTCCTCAC 
AACCCAATAT TTGTTTATAA AGTTGTGATG GATGGGAAGG AATACCCTGA AGCACAGGGA 
AGAAACGCTA AAGAAGCGAA ACAAAATGCA GCTCAACACG CTTGGAGTGA AATTCGGGAT 
CAGTCTGGCT GGACCACACC GAGTTCTGAA GATGGCGCAT TCTCACAATC CCAAGATACA 
TGTAAATCTG AAGACGTCCA GCGATCAGCG AACGCCTCCA GCACAAGTAA TTTCATCTCC 
TTCAGAGACT CTGCTGCTGC TGCCAGTTCT CCAATGGCCA CCAGTCCTTC TGTGAGCCTA 
GTGGATGTGA AGCCAAAAAT AAAATTAGCA CCAAGATTTC TGTTACCAAA CGGTCTGGAC 
AGAAGTAAAG AGGGTGGTCC TGACATGAAG GTACCAAACC CAGCAAAGGC ATCTGGAGGT 
CAAACTCCAA ACAGTGCTAC TAAATCAAGG TTTTTAGAAG ACTTTGACTC AATAAATCCG 
ATCGGGAAAG GTGGCTTTGG TCGAGTTTTC AAGGCAAGAC GAAAGCTTGA AGACAAATAT 
TATGCCGTGA AGATAGTGAA AAGTACAGAG AAAGCTCGTC GTGAGGTTGG TGCTTTGGCT 
GATTTTAATA ATCCCAACAT CGTCCGCTAC TTCTCGTCTT GGGAAGAGGA CACAGCATAC 
AAACACGAGT CGTCAGAGAG CTTCTCAGAC TCTGGCAGCG GCCCGGGAAC AAAGTTTCTC 
TACATTCAAA TGGAGTTTTG TGAAGGAAAC ACACTCCGAG TCTGGATTAA AGAAAGAAAC 
TCCTCAAACA AACAGTCCCC GGAGCGAAGA ACAGAAGCAG CTCAGATCTA CCGGCAGGTG 
CTGAAGGCTG TGGAGTACAT TCACTCTAAA TTATTGATCC ACAGAGACCT GAAGCCTCCA 
AACATAATGT TCAGCAGTGA GGGAAGAGTA AAGGTTGGAG ACTTTGGCCT CGTGACTGCG 
GCAGAAAATG AAAATGAGGA GCAACTGCTG GAACGAACTA AAAGGACGGG AACACGTACC 
TACATGAGCC CAGAGCAGAT GAATCAAACA ACATATGACA GAAAGGTGGA CATCTACGCT 
CTGGGTCTCA TCTATTTTGA ACTTGTCTGG AATCTTGGCA CAATGCATGA AAAGGAAAAG 
ATATGGGATA AGTTAAGAGT GAGGGTTTTT CCAGTGCAGT TCACAAAGAT GTTTATCTTT 
GAGCACAAGT TGATGGAGCG AATGCTGAGT CCCAGACCTG AAGACAGACC AGATGCCACA 
GACCTGATCC TGGATCTGGA TCAACATTAC ACACTCCTGA AGACGGATCA AGACCTCAGG 
ACCGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eif2ak2 ( XM_017358660.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214149.1 Danio rerio eukaryotic translation initiation factor 2-alpha kinase 2 (eif2ak2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa54641
Clone ID Related Accession (Same CDS sequence)	XM_017358660.1
Accession Version	XM_017358660.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	interferon-induced, double-stranded RNA-activated protein kinase isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCTC TGTCAGGAAA TTATACTTCT CTGCTAAATG AGTATCAGCA GAAAACCCAA 
TGCACTGTTG AGTTTGAGGA GGGACCCACT GATGGACCAA GCCACAATAA AAGGTTTACT 
ATGAGAGCTA TTGTGAATGG ACAGAAGTTT CCTGATGGTA CTGGTAAAAC CAAGAAGGAG 
GCAAAGCAAA ATGCTGCTAA AAACGCCCTG GAAGGCTTGA AGAGCACTCA CAGTGATGAA 
CCGACTCCTT CACCCGTTGA AAACATTTCA GTTAGTAAAA TAGCATCTCA TCCGAATTAC 
ACCTGCTGGC TCAATGAGCA TTCGCAGAAG AGCAGGTTAA TGTTCAAGGC CTGCGAGTCG 
ACTAAGATGG ATCCAGGAAA CCTCACCCGG TTATGCACGT ATGTGTGTAA GTATGTTTGT 
GATGATAAAG AGTTTCCTGA AGGTTATGGG AAAAATAAGA AGGAAGCCAA AGAGGCAGCG 
GCTCTTCAAG TTTATGAAGA GCTCAACAGA ACACCAAATA CAGAGGTCTT GGATGAGAAC 
AGCCATAGAG CTCAGAGGTC AGAAACTACA TCTTATAGCC TGGACCGCCG TTCGAGTGTT 
GCAGATGATT CTCGAAGCAG CACGCCTGAC AACAACTACA TCGCATATCT CAATAACTAC 
TGCCAGAAGA AAAAACGTGT ATATGACTTC AAATTAGTGG ACAGAATAGG TCCTCCTCAC 
AACCCAATAT TTGTTTATAA AGTTGTGATG GATGGGAAGG AATACCCTGA AGCACAGGGA 
AGAAACGCTA AAGAAGCGAA ACAAAATGCA GCTCAACACG CTTGGAGTGA AATTCGGGAT 
CAGTCTGGCT GGACCACACC GAGTTCTGAA GATGGCGCAT TCTCACAATC CCAAGATACA 
TGTAAATCTG AAGACGTCCA GCGCTCAGCG AACGCCTCCA GCACAAGTAA TTTCATCTCC 
TTCAGAGACT CTGCTGCTGC TGCCAGTTCT CCAATGGCCA CTAGTCCTGG TGGTCCTGAC 
ATGAAGGTAC CAAACCCAGC AAAGGCATCT GGAGGTCAAA CTCCAAACAG TGCTACTAAA 
TCAAGGTTTT TAGAAGACTT TGACTCAATA AATCCGATTG GGAAAGGTGG CTTTGGTCGA 
GTTTTCAAGG CAAGACGAAA GCTTGAAGAC AAATATTATG CCGTGAAGAT AGTGAAAAGT 
ACAGAGAAAG CTCGTCGTGA GGTTGGTGCT TTGGCTGATT TTAATAATCC CAACATCGTC 
CGCTACTTCT CGTCTTGGGA AGAGGACACA GCATACAAAC ACGAGTCGTC AGAGAGCTTC 
TCAGACTCTG GCAGCGGCCC GGGAACAAAG TTTCTCTACA TTCAAATGGA GTTTTGTGAA 
GGAAACACAC TTCGAGTCTG GATTAAAGAA AGAAACTCCT CAAGCAAACA GTCCCCGGAG 
CGAAGAACAG AAGCAGCTCA GATCTACCGG CAGGTGCTGA AGGCTGTGGA GTACATTCAC 
TCAAAATCAT TGATCCACAG AGACCTGAAG CCTCCAAACA TAATGTTCAG CAGTGAGGGA 
AGAGTAAAGG TTGGAGACTT TGGCCTCGTG ACTGCGGCAG AAAATGAAAA TGAGGAGCAA 
CTGCTGGAAC GAACTAAAAG GACGGGAACA CGTACCTACA TGAGCCCAGA GCAGATGAAT 
CAAACAACAT ATGACAGAAA GGTGGACATC TACGCTCTGG GTCTCATCTA TTTTGAACTT 
GTCTGGAATC TTGGCACAAT GCATGAAAAG GAAAAGATAT GGGATAAGTT AAGAGTGAGG 
GTTTTTCCAG TGCAGTTCAC AAAGATGTTT ATCTTTGAGC ACAAGTTGAT GGAGCGAATG 
CTGAGTCCCA GACCTGAAGA CAGACCAGAT GCCACAGACC TGATCCTGGA TCTGGATCAA 
CATTACACAC TCCTGAAGAC TGATCAAGAC CTCAGGACCG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017214149.1
CDS	229..2193
Translation	MESLSGNYTSLLNEYQQKTQCTVEFEEGPTDGPSHNKRFTMRAIVNGQKFPDGTGKTKKEAKQNAAKNALEGLKSTHSDEPTPSPVENISVSKIASHPNYTCWLNEHSQKSRLMFKACESTKMDPGNLTRLCTYVCKYVCDDKEFPEGYGKNKKEAKEAAALQVYEELNRTPNTEVLDENSHRAQRSETTSYSLDRRSSVADDSRSSTPDNNYIAYLNNYCQKKKRVYDFKLVDRIGPPHNPIFVYKVVMDGKEYPEAQGRNAKEAKQNAAQHAWSEIRDQSGWTTPSSEDGAFSQSQDTCKSEDVQRSANASSTSNFISFRDSAAAASSPMATSPGGPDMKVPNPAKASGGQTPNSATKSRFLEDFDSINPIGKGGFGRVFKARRKLEDKYYAVKIVKSTEKARREVGALADFNNPNIVRYFSSWEEDTAYKHESSESFSDSGSGPGTKFLYIQMEFCEGNTLRVWIKERNSSSKQSPERRTEAAQIYRQVLKAVEYIHSKSLIHRDLKPPNIMFSSEGRVKVGDFGLVTAAENENEEQLLERTKRTGTRTYMSPEQMNQTTYDRKVDIYALGLIYFELVWNLGTMHEKEKIWDKLRVRVFPVQFTKMFIFEHKLMERMLSPRPEDRPDATDLILDLDQHYTLLKTDQDLRTV

Target ORF information:

RefSeq Version	XM_017358660.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio eukaryotic translation initiation factor 2-alpha kinase 2 (eif2ak2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358660.1

ORF Insert Sequence:

ATGGAGTCTC TGTCAGGAAA TTATACTTCT CTGCTAAATG AGTATCAGCA GAAAACCCAA 
TGCACTGTTG AGTTTGAGGA GGGACCCACT GATGGACCAA GCCACAATAA AAGGTTTACT 
ATGAGAGCTA TTGTGAATGG ACAGAAGTTT CCTGATGGTA CTGGTAAAAC CAAGAAGGAG 
GCAAAGCAAA ATGCTGCTAA AAACGCCCTG GAAGGCTTGA AGAGCACTCA CAGTGATGAA 
CCGACTCCTT CACCCGTTGA AAACATTTCA GTTAGTAAAA TAGCATCTCA TCCGAATTAC 
ACCTGCTGGC TCAATGAGCA TTCGCAGAAG AGCAGGTTAA TGTTCAAGGC CTGCGAGTCG 
ACTAAGATGG ATCCAGGAAA CCTCACCCGG TTATGCACGT ATGTGTGTAA GTATGTTTGT 
GATGATAAAG AGTTTCCTGA AGGTTATGGG AAAAATAAGA AGGAAGCCAA AGAGGCAGCG 
GCTCTTCAAG TTTATGAAGA GCTCAACAGA ACACCAAATA CAGAGGTCTT GGATGAGAAC 
AGCCATAGAG CTCAGAGGTC AGAAACTACA TCTTATAGCC TGGACCGCCG TTCGAGTGTT 
GCAGATGATT CTCGAAGCAG CACGCCTGAC AACAACTACA TCGCATATCT CAATAACTAC 
TGCCAGAAGA AAAAACGTGT ATATGACTTC AAATTAGTGG ACAGAATAGG TCCTCCTCAC 
AACCCAATAT TTGTTTATAA AGTTGTGATG GATGGGAAGG AATACCCTGA AGCACAGGGA 
AGAAACGCTA AAGAAGCGAA ACAAAATGCA GCTCAACACG CTTGGAGTGA AATTCGGGAT 
CAGTCTGGCT GGACCACACC GAGTTCTGAA GATGGCGCAT TCTCACAATC CCAAGATACA 
TGTAAATCTG AAGACGTCCA GCGCTCAGCG AACGCCTCCA GCACAAGTAA TTTCATCTCC 
TTCAGAGACT CTGCTGCTGC TGCCAGTTCT CCAATGGCCA CTAGTCCTGG TGGTCCTGAC 
ATGAAGGTAC CAAACCCAGC AAAGGCATCT GGAGGTCAAA CTCCAAACAG TGCTACTAAA 
TCAAGGTTTT TAGAAGACTT TGACTCAATA AATCCGATTG GGAAAGGTGG CTTTGGTCGA 
GTTTTCAAGG CAAGACGAAA GCTTGAAGAC AAATATTATG CCGTGAAGAT AGTGAAAAGT 
ACAGAGAAAG CTCGTCGTGA GGTTGGTGCT TTGGCTGATT TTAATAATCC CAACATCGTC 
CGCTACTTCT CGTCTTGGGA AGAGGACACA GCATACAAAC ACGAGTCGTC AGAGAGCTTC 
TCAGACTCTG GCAGCGGCCC GGGAACAAAG TTTCTCTACA TTCAAATGGA GTTTTGTGAA 
GGAAACACAC TTCGAGTCTG GATTAAAGAA AGAAACTCCT CAAGCAAACA GTCCCCGGAG 
CGAAGAACAG AAGCAGCTCA GATCTACCGG CAGGTGCTGA AGGCTGTGGA GTACATTCAC 
TCAAAATCAT TGATCCACAG AGACCTGAAG CCTCCAAACA TAATGTTCAG CAGTGAGGGA 
AGAGTAAAGG TTGGAGACTT TGGCCTCGTG ACTGCGGCAG AAAATGAAAA TGAGGAGCAA 
CTGCTGGAAC GAACTAAAAG GACGGGAACA CGTACCTACA TGAGCCCAGA GCAGATGAAT 
CAAACAACAT ATGACAGAAA GGTGGACATC TACGCTCTGG GTCTCATCTA TTTTGAACTT 
GTCTGGAATC TTGGCACAAT GCATGAAAAG GAAAAGATAT GGGATAAGTT AAGAGTGAGG 
GTTTTTCCAG TGCAGTTCAC AAAGATGTTT ATCTTTGAGC ACAAGTTGAT GGAGCGAATG 
CTGAGTCCCA GACCTGAAGA CAGACCAGAT GCCACAGACC TGATCCTGGA TCTGGATCAA 
CATTACACAC TCCTGAAGAC TGATCAAGAC CTCAGGACCG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RNF135 is a positive regulator of IFN expression and involved in RIG-I signaling pathway by targeting RIG-I.
Fish & shellfish immunology86474-479(2019 Mar)
Lai Y,Liang M,Hu,Zeng Z,Lin H,Yi G,Li M,Liu Z

NOD1 deficiency impairs CD44a/Lck as well as PI3K/Akt pathway.
Scientific reports7(1)2979(2017 06)
Hu YW,Wu XM,Ren SS,Cao L,Nie P,Chang MX

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Contrasted innate responses to two viruses in zebrafish: insights into the ancestral repertoire of vertebrate IFN-stimulated genes.
Journal of immunology (Baltimore, Md. : 1950)192(9)4328-41(2014 May)
Briolat V,Jouneau L,Carvalho R,Palha N,Langevin C,Herbomel P,Schwartz O,Spaink HP,Levraud JP,Boudinot P

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eif2ak2 cDNA ORF clone, Danio rerio(zebrafish)

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