SHARPIN cDNA ORF clone, Canis lupus familiaris(Dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	SHARPIN
Entrez Gene ID	475122
Full Name	SHANK associated RH domain interactor
General protein information	Preferred Names SHANK associated RH domain interactor Names sharpin
Gene Type	protein-coding
Organism	Canis lupus familiaris(Dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014118748.1	XP_013974223.1	sharpin isoform X1
XM_532352.5	XP_532352.3	sharpin isoform X2
XM_532352.6	XP_532352.4	sharpin
XM_005628075.2	XP_005628132.1	sharpin isoform X3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SHARPIN	XP_001156216.2
Mus musculus (house mouse)	Sharpin	NP_079616.2
Homo sapiens (human)	SHARPIN	NP_112236.3
Rattus norvegicus (Norway rat)	Sharpin	NP_112415.1
Danio rerio (zebrafish)	LOC793673	XP_001333481.5
Macaca mulatta (Rhesus monkey)	LOC713534	XP_001102921.2
Bos taurus (cattle)	SHARPIN	NP_001103236.1
Canis lupus familiaris (dog)	SHARPIN	XP_532352.3

Gene Ontology

The following SHARPIN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SHARPIN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe292947	XM_532352.6 Latest version!	Canis lupus familiaris SHANK associated RH domain interactor (SHARPIN), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OCe16163	XM_532352.5 Latest version!	Canis lupus familiaris SHANK-associated RH domain interactor (SHARPIN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OCe16164	XM_005628075.2 Latest version!	Canis lupus familiaris SHANK-associated RH domain interactor (SHARPIN), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OCe39772	XM_014118748.1 Latest version!	Canis lupus familiaris SHANK-associated RH domain interactor (SHARPIN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SHARPIN ( XM_532352.6 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_532352.4 Canis lupus familiaris SHANK associated RH domain interactor (SHARPIN), mRNA.

Price & Availability↑

CloneID	OCe292947
Clone ID Related Accession (Same CDS sequence)	XM_532352.6
Accession Version	XM_532352.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1197bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	sharpin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006595.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_532352.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCCGC CCTCGGGCCC GGGCCCGGCC GCCGTGCTGC TGGCGGTGCA GGCCACGGTG 
AGGCCGCTGG GCGCGGGCCC GGACGCCGAG GCGCAGCTGC GGAGGCTGCA GCTGAGCGCG 
GACCCAGAGC GGCCCGGGCG TCTCCGGCTG CAGCTGCTGG GCGCGGGCCC CGGGGCGGGC 
GGTTCGGAGT GGCCCTTGGA GTCCGTCTCC TACACGGTCC GAGGCCCCAG CCGGCATGAG 
CTGCAGCCTC CGCCGGGAGG GCCCGGCGCC CTGAGCCTGC ACTTCCTCGA CGCCCGGGAC 
GCTCGGCGCT GGGCCGCCCT GGTCCGACGG GCCGCCATGG AGGGACGCGA TGGCAGCCTG 
CCCCAAGCCC TGGGCCCAGA CACATGCCCT GTGTCCCCAC CCAGCCCCCC CGAAGTGCCC 
ACACTCCAGA CCCCACAACC CAAGGTGGAT CTTTGCTGGA GCCCTGGAGA CTCTCCGGAG 
AAAGAGGAGC TGGTGGGGCG CCTGGCCCGG GCCATCGAGG ACGGAGATGA GAAGGGGGCA 
GCCCAGGCAG CAGCCCTGCT GGCCCAGCAT CACGTGGCCC TCAGCGTCCG GCTCCGGGAG 
GCCTGCTTCC CTCCTGGCCC CATCAGGCTA CAGGTCACAG TTGAAGATGC TGCGTCCTCT 
GCCCACGTGT CCCTGCAAGT TCACCCCCAT TGCACCATCG CAACTCTCCA GGAGCAGGTG 
TTCTCGGAGT TTGGCTTCCC GCCAGCTGTG CAGCGCTGGG TCATCGGGCA GTGCCTGTGT 
GTGCCCGAGC GCAGCCTTGC TTCGTATGGG GTCCGGCGGG ATGGGGATCC TGCTTTCCTC 
TATCTGCTCT CAGCGCCCGG AGAGGCCTCA GGACGCAGCC CTCTGGGCCC CCAGAAGACA 
GATGGGGAAC TAAGTCGCCT GTTTCCGCAG TCACTGGGGC TGCCCAGAGC GCCTCAGCCA 
GCCAGCCTCC CCAGCCCCCT TCAGCCTGGC TGGTCCTGCC CTTCCTGCAC ATTCATCAAT 
GCCCCCAGCC GACCCGGCTG TGAGATGTGC AGCACCCAGA AGCCCTGCAG TTGGGACCCC 
CTTCCTGCAG CCTCCACCCA CCAGCCGCCA AAGGTCTCGA AGAGAGAGGA TGGCCTGATC 
CCTCCGGGCC CGAGGTCCCT GGAGCCTGTC CTGAAACTCT CAGGCGACTT TTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_532352.4
CDS	441..1637
Translation	MAPPSGPGPAAVLLAVQATVRPLGAGPDAEAQLRRLQLSADPERPGRLRLQLLGAGPGAGGSEWPLESVSYTVRGPSRHELQPPPGGPGALSLHFLDARDARRWAALVRRAAMEGRDGSLPQALGPDTCPVSPPSPPEVPTLQTPQPKVDLCWSPGDSPEKEELVGRLARAIEDGDEKGAAQAAALLAQHHVALSVRLREACFPPGPIRLQVTVEDAASSAHVSLQVHPHCTIATLQEQVFSEFGFPPAVQRWVIGQCLCVPERSLASYGVRRDGDPAFLYLLSAPGEASGRSPLGPQKTDGELSRLFPQSLGLPRAPQPASLPSPLQPGWSCPSCTFINAPSRPGCEMCSTQKPCSWDPLPAASTHQPPKVSKREDGLIPPGPRSLEPVLKLSGDFC

Target ORF information:

RefSeq Version	XM_532352.6
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris SHANK associated RH domain interactor (SHARPIN), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_532352.6

ORF Insert Sequence:

ATGGCGCCGC CCTCGGGCCC GGGCCCGGCC GCCGTGCTGC TGGCGGTGCA GGCCACGGTG 
AGGCCGCTGG GCGCGGGCCC GGACGCCGAG GCGCAGCTGC GGAGGCTGCA GCTGAGCGCG 
GACCCAGAGC GGCCCGGGCG TCTCCGGCTG CAGCTGCTGG GCGCGGGCCC CGGGGCGGGC 
GGTTCGGAGT GGCCCTTGGA GTCCGTCTCC TACACGGTCC GAGGCCCCAG CCGGCATGAG 
CTGCAGCCTC CGCCGGGAGG GCCCGGCGCC CTGAGCCTGC ACTTCCTCGA CGCCCGGGAC 
GCTCGGCGCT GGGCCGCCCT GGTCCGACGG GCCGCCATGG AGGGACGCGA TGGCAGCCTG 
CCCCAAGCCC TGGGCCCAGA CACATGCCCT GTGTCCCCAC CCAGCCCCCC CGAAGTGCCC 
ACACTCCAGA CCCCACAACC CAAGGTGGAT CTTTGCTGGA GCCCTGGAGA CTCTCCGGAG 
AAAGAGGAGC TGGTGGGGCG CCTGGCCCGG GCCATCGAGG ACGGAGATGA GAAGGGGGCA 
GCCCAGGCAG CAGCCCTGCT GGCCCAGCAT CACGTGGCCC TCAGCGTCCG GCTCCGGGAG 
GCCTGCTTCC CTCCTGGCCC CATCAGGCTA CAGGTCACAG TTGAAGATGC TGCGTCCTCT 
GCCCACGTGT CCCTGCAAGT TCACCCCCAT TGCACCATCG CAACTCTCCA GGAGCAGGTG 
TTCTCGGAGT TTGGCTTCCC GCCAGCTGTG CAGCGCTGGG TCATCGGGCA GTGCCTGTGT 
GTGCCCGAGC GCAGCCTTGC TTCGTATGGG GTCCGGCGGG ATGGGGATCC TGCTTTCCTC 
TATCTGCTCT CAGCGCCCGG AGAGGCCTCA GGACGCAGCC CTCTGGGCCC CCAGAAGACA 
GATGGGGAAC TAAGTCGCCT GTTTCCGCAG TCACTGGGGC TGCCCAGAGC GCCTCAGCCA 
GCCAGCCTCC CCAGCCCCCT TCAGCCTGGC TGGTCCTGCC CTTCCTGCAC ATTCATCAAT 
GCCCCCAGCC GACCCGGCTG TGAGATGTGC AGCACCCAGA AGCCCTGCAG TTGGGACCCC 
CTTCCTGCAG CCTCCACCCA CCAGCCGCCA AAGGTCTCGA AGAGAGAGGA TGGCCTGATC 
CCTCCGGGCC CGAGGTCCCT GGAGCCTGTC CTGAAACTCT CAGGCGACTT TTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SHARPIN ( XM_532352.5 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_532352.3 Canis lupus familiaris SHANK-associated RH domain interactor (SHARPIN), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe16163
Clone ID Related Accession (Same CDS sequence)	XM_532352.5
Accession Version	XM_532352.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2583bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	sharpin isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726083.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_532352.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAGGGT CCGGGGCTTC ATCTCCCGGG ATACGGCCGT CCCGCCGCAA GAGCATCACC 
CACCATGGGG GGTGGGGGGC GTCGCCGCGA GCCCGCGGGA TCCCTGCAGT AGCCCAGGGG 
CCGACTCCTG CGCCCGGTGA GGACCCGAAC ACCGAGGCGG GGGCCTGCGG TCGGTCACCT 
GGGCAGCGCT CTCCCCTGTC CTACGGGCTC CCCAGGGAGG CGCACAGGAA ACCCATAGGC 
TTCTCCGTTC CTCTCCGCAA GTGCGCCGAG CTCCTCGGAA CAGAGGCAGA GGCAGGCCGC 
AGGACCCCCG TAGGGCCCGG GGCCGGCCGC GGGGCGCTCC GGGGTCAGGC GGCCGAACCC 
GCCTCTCCGC CCGGGGCCAA GGAAAGCCCC GAGCCCGCCG CGGAGAACTA CACGTCCCAG 
GAGGCCGCGC GCCCCGGGCC GCGCACGCGC GCCGGCGCCA AGTCCGCTGG GGGCTGGAGT 
TCTGGAGGCC TGGCCTGGGC GCCCGGCGCC GGGCGGACAC CCACCTGCTG GCGCGGCGCT 
CGCGGGGGCC GGGCCGGGTC GGGCCGGCGG ACGTCGGCGC GGGTAAGAGG GCGCTGTGGG 
CTCCGGGCAG CGCGGGCTCC TCAGGACCGG AGGGCGGCGG CGCGGCCTCC GTTCGGGCCG 
CACTGTATCC GGGGGAAGAG GCTCCGCGGC GACGTCCCGT TACTCCCGGC CCTGCCGGCC 
CTGCCGGGCT GCCACCGCGA CATAGCCTCG CCCCTGCACG GCGACCGACT GCCGCGACCG 
ACCTGCCCGC CGACCGCCAC CCGAGGCCCG GACGAGCGCA GGAGCGGCCG AGCGACCTCT 
GCTTCCCCGG GCCGGACAAC AACAAGCGTC CGCAAGGCCC GGCCCCCCGC CCCTGAGTGG 
CCCGCTCCGG CCGTCGGGAC CTGGAGCTCG GAGCGCCATT GGGGACGGCT GACGTCGCTC 
ACTCCTCGCG TCCGCTCCGA CTGGCTGCGC CGCCTGTCCG TCACGCTCCG GGCCGCGCGA 
GCCCGCCTCC TCCCCTGGCG CTCATTGGCT GCCACCCCTC GCGCGTCCCG CCCCTGCTGT 
CGGCCCGCGC TCCCGAGAGT GGCTGGCGGC CCCCGGGGTG GGCCGGCGGG GCGGGGGGCG 
GGCCGGAAGG AGCCCGGGGA CTGCGTCCCG CACCGGCCGG GCGCGGGGGG GCGGGCCCGG 
ACCGTTGGAC CACGCCTCCG CCGCCGAGCC TCCGGGCCGT GTGGGTCCCG GGCCCAGCGG 
GACCGGGAGG CTGGCGCTCC GACCCTCTGG TGTCGGCCGG AACCCCAAGC GCGCGGCCCC 
AGTTTCCGGT CGGCCGAGGC GCTCGGAGCC CCGAGCCCAC GGAAGCGCGG CCGGACCGGA 
GATGGGATGG CGCCGCCCTC GGGCCCGGGC CCGGCCGCCG TGCTGCTGGC GGTGCAGGCC 
ACGGTGAGGC CGCTGGGCGC GGGCCCGGAC GCCGAGGCGC AGCTGCGGAG GCTGCAGCTG 
AGCGCGGACC CAGAGCGGCC CGGGCGTCTC CGGCTGCAGC TGCTGGGCGC GGGCCCCGGG 
GCGGGCGGTT CGGAGTGGCC CTTGGAGTCC GTCTCCTACA CGGTCCGAGG CCCCAGCCGG 
CATGAGCTGC AGCCTCCGCC GGGAGGGCCC GGCGCCCTGA GCCTGCACTT CCTCGACGCC 
CGGGACGCTC GGCGCTGGGC CGCCCTGGTC CGACGGGCCG CCATGGAGGG ACGCGATGGC 
AGCCTGCCCC AAGCCCTGGG CCCAGACACA TGCCCTGTGT CCCCACCCAG CCCCCCCGAA 
GTGCCCACAC TCCAGACCCC ACAACCCAAG GTGGATCTTT GCTGGAGCCC TGGAGACTCT 
CCGGAGAAAG AGGAGCTGGT GGGGCGCCTG GCCCGGGCCA TCGAGGACGG AGATGAGAAG 
GGGGCAGCCC AGGCAGCAGC CCTGCTGGCC CAGCATCACG TGGCCCTCAG CGTCCGGCTC 
CGGGAGGCCT GCTTCCCTCC TGGCCCCATC AGGCTACAGG TCACAGTTGA AGATGCTGCG 
TCCTCTGCCC ACGTGTCCCT GCAAGTTCAC CCCCATTGCA CCATCGCAAC TCTCCAGGAG 
CAGGTGTTCT CGGAGTTTGG CTTCCCGCCA GCTGTGCAGC GCTGGGTCAT CGGGCAGTGC 
CTGTGTGTGC CCGAGCGCAG CCTTGCTTCG TATGGGGTCC GGCGGGATGG GGATCCTGCT 
TTCCTCTATC TGCTCTCAGC GCCCGGAGAG GCCTCAGGAC GCAGCCCTCT GGGCCCCCAG 
AAGACAGATG GGGAACTAAG TCGCCTGTTT CCGCAGTCAC TGGGGCTGCC CAGAGCGCCT 
CAGCCAGCCA GCCTCCCCAG CCCCCTTCAG CCTGGCTGGT CCTGCCCTTC CTGCACATTC 
ATCAATGCCC CCAGCCGACC CGGCTGTGAG ATGTGCAGCA CCCAGAAGCC CTGCAGTTGG 
GACCCCCTTC CTGCAGCCTC CACCCACCAG CCGCCAAAGG TCTCGAAGAG AGAGGATGGC 
CTGATCCCTC CGGGCCCGAG GTCCCTGGAG CCTGTCCTGA AACTCTCAGG CGACTTTTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_532352.3
CDS	265..2847
Translation	MLGSGASSPGIRPSRRKSITHHGGWGASPRARGIPAVAQGPTPAPGEDPNTEAGACGRSPGQRSPLSYGLPREAHRKPIGFSVPLRKCAELLGTEAEAGRRTPVGPGAGRGALRGQAAEPASPPGAKESPEPAAENYTSQEAARPGPRTRAGAKSAGGWSSGGLAWAPGAGRTPTCWRGARGGRAGSGRRTSARVRGRCGLRAARAPQDRRAAARPPFGPHCIRGKRLRGDVPLLPALPALPGCHRDIASPLHGDRLPRPTCPPTATRGPDERRSGRATSASPGRTTTSVRKARPPAPEWPAPAVGTWSSERHWGRLTSLTPRVRSDWLRRLSVTLRAARARLLPWRSLAATPRASRPCCRPALPRVAGGPRGGPAGRGAGRKEPGDCVPHRPGAGGRARTVGPRLRRRASGPCGSRAQRDREAGAPTLWCRPEPQARGPSFRSAEALGAPSPRKRGRTGDGMAPPSGPGPAAVLLAVQATVRPLGAGPDAEAQLRRLQLSADPERPGRLRLQLLGAGPGAGGSEWPLESVSYTVRGPSRHELQPPPGGPGALSLHFLDARDARRWAALVRRAAMEGRDGSLPQALGPDTCPVSPPSPPEVPTLQTPQPKVDLCWSPGDSPEKEELVGRLARAIEDGDEKGAAQAAALLAQHHVALSVRLREACFPPGPIRLQVTVEDAASSAHVSLQVHPHCTIATLQEQVFSEFGFPPAVQRWVIGQCLCVPERSLASYGVRRDGDPAFLYLLSAPGEASGRSPLGPQKTDGELSRLFPQSLGLPRAPQPASLPSPLQPGWSCPSCTFINAPSRPGCEMCSTQKPCSWDPLPAASTHQPPKVSKREDGLIPPGPRSLEPVLKLSGDFC

Target ORF information:

RefSeq Version	XM_532352.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris SHANK-associated RH domain interactor (SHARPIN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_532352.5

ORF Insert Sequence:

ATGCTAGGGT CCGGGGCTTC ATCTCCCGGG ATACGGCCGT CCCGCCGCAA GAGCATCACC 
CACCATGGGG GGTGGGGGGC GTCGCCGCGA GCCCGCGGGA TCCCTGCAGT AGCCCAGGGG 
CCGACTCCTG CGCCCGGTGA GGACCCGAAC ACCGAGGCGG GGGCCTGCGG TCGGTCACCT 
GGGCAGCGCT CTCCCCTGTC CTACGGGCTC CCCAGGGAGG CGCACAGGAA ACCCATAGGC 
TTCTCCGTTC CTCTCCGCAA GTGCGCCGAG CTCCTCGGAA CAGAGGCAGA GGCAGGCCGC 
AGGACCCCCG TAGGGCCCGG GGCCGGCCGC GGGGCGCTCC GGGGTCAGGC GGCCGAACCC 
GCCTCTCCGC CCGGGGCCAA GGAAAGCCCC GAGCCCGCCG CGGAGAACTA CACGTCCCAG 
GAGGCCGCGC GCCCCGGGCC GCGCACGCGC GCCGGCGCCA AGTCCGCTGG GGGCTGGAGT 
TCTGGAGGCC TGGCCTGGGC GCCCGGCGCC GGGCGGACAC CCACCTGCTG GCGCGGCGCT 
CGCGGGGGCC GGGCCGGGTC GGGCCGGCGG ACGTCGGCGC GGGTAAGAGG GCGCTGTGGG 
CTCCGGGCAG CGCGGGCTCC TCAGGACCGG AGGGCGGCGG CGCGGCCTCC GTTCGGGCCG 
CACTGTATCC GGGGGAAGAG GCTCCGCGGC GACGTCCCGT TACTCCCGGC CCTGCCGGCC 
CTGCCGGGCT GCCACCGCGA CATAGCCTCG CCCCTGCACG GCGACCGACT GCCGCGACCG 
ACCTGCCCGC CGACCGCCAC CCGAGGCCCG GACGAGCGCA GGAGCGGCCG AGCGACCTCT 
GCTTCCCCGG GCCGGACAAC AACAAGCGTC CGCAAGGCCC GGCCCCCCGC CCCTGAGTGG 
CCCGCTCCGG CCGTCGGGAC CTGGAGCTCG GAGCGCCATT GGGGACGGCT GACGTCGCTC 
ACTCCTCGCG TCCGCTCCGA CTGGCTGCGC CGCCTGTCCG TCACGCTCCG GGCCGCGCGA 
GCCCGCCTCC TCCCCTGGCG CTCATTGGCT GCCACCCCTC GCGCGTCCCG CCCCTGCTGT 
CGGCCCGCGC TCCCGAGAGT GGCTGGCGGC CCCCGGGGTG GGCCGGCGGG GCGGGGGGCG 
GGCCGGAAGG AGCCCGGGGA CTGCGTCCCG CACCGGCCGG GCGCGGGGGG GCGGGCCCGG 
ACCGTTGGAC CACGCCTCCG CCGCCGAGCC TCCGGGCCGT GTGGGTCCCG GGCCCAGCGG 
GACCGGGAGG CTGGCGCTCC GACCCTCTGG TGTCGGCCGG AACCCCAAGC GCGCGGCCCC 
AGTTTCCGGT CGGCCGAGGC GCTCGGAGCC CCGAGCCCAC GGAAGCGCGG CCGGACCGGA 
GATGGGATGG CGCCGCCCTC GGGCCCGGGC CCGGCCGCCG TGCTGCTGGC GGTGCAGGCC 
ACGGTGAGGC CGCTGGGCGC GGGCCCGGAC GCCGAGGCGC AGCTGCGGAG GCTGCAGCTG 
AGCGCGGACC CAGAGCGGCC CGGGCGTCTC CGGCTGCAGC TGCTGGGCGC GGGCCCCGGG 
GCGGGCGGTT CGGAGTGGCC CTTGGAGTCC GTCTCCTACA CGGTCCGAGG CCCCAGCCGG 
CATGAGCTGC AGCCTCCGCC GGGAGGGCCC GGCGCCCTGA GCCTGCACTT CCTCGACGCC 
CGGGACGCTC GGCGCTGGGC CGCCCTGGTC CGACGGGCCG CCATGGAGGG ACGCGATGGC 
AGCCTGCCCC AAGCCCTGGG CCCAGACACA TGCCCTGTGT CCCCACCCAG CCCCCCCGAA 
GTGCCCACAC TCCAGACCCC ACAACCCAAG GTGGATCTTT GCTGGAGCCC TGGAGACTCT 
CCGGAGAAAG AGGAGCTGGT GGGGCGCCTG GCCCGGGCCA TCGAGGACGG AGATGAGAAG 
GGGGCAGCCC AGGCAGCAGC CCTGCTGGCC CAGCATCACG TGGCCCTCAG CGTCCGGCTC 
CGGGAGGCCT GCTTCCCTCC TGGCCCCATC AGGCTACAGG TCACAGTTGA AGATGCTGCG 
TCCTCTGCCC ACGTGTCCCT GCAAGTTCAC CCCCATTGCA CCATCGCAAC TCTCCAGGAG 
CAGGTGTTCT CGGAGTTTGG CTTCCCGCCA GCTGTGCAGC GCTGGGTCAT CGGGCAGTGC 
CTGTGTGTGC CCGAGCGCAG CCTTGCTTCG TATGGGGTCC GGCGGGATGG GGATCCTGCT 
TTCCTCTATC TGCTCTCAGC GCCCGGAGAG GCCTCAGGAC GCAGCCCTCT GGGCCCCCAG 
AAGACAGATG GGGAACTAAG TCGCCTGTTT CCGCAGTCAC TGGGGCTGCC CAGAGCGCCT 
CAGCCAGCCA GCCTCCCCAG CCCCCTTCAG CCTGGCTGGT CCTGCCCTTC CTGCACATTC 
ATCAATGCCC CCAGCCGACC CGGCTGTGAG ATGTGCAGCA CCCAGAAGCC CTGCAGTTGG 
GACCCCCTTC CTGCAGCCTC CACCCACCAG CCGCCAAAGG TCTCGAAGAG AGAGGATGGC 
CTGATCCCTC CGGGCCCGAG GTCCCTGGAG CCTGTCCTGA AACTCTCAGG CGACTTTTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SHARPIN ( XM_005628075.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005628132.1 Canis lupus familiaris SHANK-associated RH domain interactor (SHARPIN), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe16164
Clone ID Related Accession (Same CDS sequence)	XM_005628075.2
Accession Version	XM_005628075.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	sharpin isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726083.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005628075.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAGGGT CCGGGGCTTC ATCTCCCGGG ATACGGCCGT CCCGCCGCAA GAGCATCACC 
CACCATGGGG GGTGGGGGGC GTCGCCGCGA GCCCGCGGGA TCCCTGCAGT AGCCCAGGGG 
CCGACTCCTG CGCCCGGTGA GGACCCGAAC ACCGAGGCGG GGGCCTGCGG TCGGTCACCT 
GGGCAGCGCT CTCCCCTGTC CTACGGGCTC CCCAGGGAGG CGCACAGGAA ACCCATAGGC 
TTCTCCGTTC CTCTCCGCAA GTGCGCCGAG CTCCTCGGAA CAGAGGCAGA GGCAGGCCGC 
AGGACCCCCG TAGGGCCCGG GGCCGGCCGC GGGGCGCTCC GGGGTCAGGC GGCCGAACCC 
GCCTCTCCGC CCGGGGCCAA GGAAAGCCCC GAGCCCGCCG CGGAGAACTA CACGTCCCAG 
GAGGCCGCGC GCCCCGGGCC GCGCACGCGC GCCGGCGCCA AGTCCGCTGG GGGCTGGAGT 
TCTGGAGGCC TGGCCTGGGC GCCCGGCGCC GGGCGGACAC CCACCTGCTG GCGCGGCGCT 
CGCGGGGGCC GGGCCGGGTC GGGCCGGCGG ACGTCGGCGC GGGTAAGAGG GCGCTGTGGG 
CTCCGGGCAG CGCGGGCTCC TCAGGACCGG AGGGCGGCGG CGCGGCCTCC GTTCGGGCCG 
CACTGTATCC GGGGGAAGAG GCTCCGCGGC GACGTCCCGT TACTCCCGGC CCTGCCGGCC 
CTGCCGGGCT GCCACCGCGA CATAGCCTCG CCCCTGCACG GCGACCGACT GCCGCGACCG 
ACCTGCCCGC CGACCGCCAC CCGAGGCCCG GACGAGCGCA GGAGCGGCCG AGCGACCTCT 
GCTTCCCCGG GCCGGACAAC AACAAGCGTC CGCAAGGCCC GGCCCCCCGC CCCTGAGTGG 
CCCGCTCCGG CCGTCGGGAC CTGGAGCTCG GAGCGCCATT GGGGACGGCT GACGTCGCTC 
ACTCCTCGCG TCCGCTCCGA CTGGCTGCGC CGCCTGTCCG TCACGCTCCG GGCCGCGCGA 
GCCCGCCTCC TCCCCTGGCG CTCATTGGCT GCCACCCCTC GCGCGTCCCG CCCCTGCTGT 
CGGCCCGCGC TCCCGAGAGT GGCTGGCGGC CCCCGGGGTG GGCCGGCGGG GCGGGGGGCG 
GGCCGGAAGG AGCCCGGGGA CTGCGTCCCG CACCGGCCGG GCGCGGGGGG GCGGGCCCGG 
ACCGTTGGAC CACGCCTCCG CCGCCGAGCC TCCGGGCCGT GTGGGTCCCG GGCCCAGCGG 
GACCGGGAGG CTGGCGCTCC GACCCTCTGG TGTCGGCCGG AACCCCAAGC GCGCGGCCCC 
AGTTTCCGGT CGGCCGAGGC GCTCGGAGCC CCGAGCCCAC GGAAGCGCGG CCGGACCGGA 
GATGGGATGG CGCCGCCCTC GGGCCCGGGC CCGGCCGCCG TGCTGCTGGC GGTGCAGGCC 
ACGGGCGGTT CGGAGTGGCC CTTGGAGTCC GTCTCCTACA CGGTCCGAGG CCCCAGCCGG 
CATGAGCTGC AGCCTCCGCC GGGAGGGCCC GGCGCCCTGA GCCTGCACTT CCTCGACGCC 
CGGGACGCTC GGCGCTGGGC CGCCCTGGTC CGACGGGCCG CCATGGAGGG ACGCGATGGC 
AGCCTGCCCC AAGCCCTGGG CCCAGACACA TGCCCTGTGT CCCCACCCAG CCCCCCCGAA 
GTGCCCACAC TCCAGACCCC ACAACCCAAG GTGGATCTTT GCTGGAGCCC TGGAGACTCT 
CCGGAGAAAG AGGAGCTGGT GGGGCGCCTG GCCCGGGCCA TCGAGGACGG AGATGAGAAG 
GGGGCAGCCC AGGCAGCAGC CCTGCTGGCC CAGCATCACG TGGCCCTCAG CGTCCGGCTC 
CGGGAGGCCT GCTTCCCTCC TGGCCCCATC AGGCTACAGG TCACAGTTGA AGATGCTGCG 
TCCTCTGCCC ACGTGTCCCT GCAAGTTCAC CCCCATTGCA CCATCGCAAC TCTCCAGGAG 
CAGGTGTTCT CGGAGTTTGG CTTCCCGCCA GCTGTGCAGC GCTGGGTCAT CGGGCAGTGC 
CTGTGTGTGC CCGAGCGCAG CCTTGCTTCG TATGGGGTCC GGCGGGATGG GGATCCTGCT 
TTCCTCTATC TGCTCTCAGC GCCCGGAGAG GCCTCAGGAC GCAGCCCTCT GGGCCCCCAG 
AAGACAGATG GGGAACTAAG TCGCCTGTTT CCGCAGTCAC TGGGGCTGCC CAGAGCGCCT 
CAGCCAGCCA GCCTCCCCAG CCCCCTTCAG GTGGGTAAGG GACTGGGCCG GGTGGGCCTG 
GGACTTGCTG GGCAGCTGAA GGATAGAGAA GCAGCCGCAG TCTCCCCGCC TCCCCAGCCT 
GGCTGGTCCT GCCCTTCCTG CACATTCATC AATGCCCCCA GCCGACCCGG CTGTGAGATG 
TGCAGCACCC AGAAGCCCTG CAGTTGGGAC CCCCTTCCTG CAGCCTCCAC CCACCAGCCG 
CCAAAGGTCT CGAAGAGAGA GGATGGCCTG ATCCCTCCGG GCCCGAGGTC CCTGGAGCCT 
GTCCTGAAAC TCTCAGGCGA CTTTTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005628132.1
CDS	265..2814
Translation	MLGSGASSPGIRPSRRKSITHHGGWGASPRARGIPAVAQGPTPAPGEDPNTEAGACGRSPGQRSPLSYGLPREAHRKPIGFSVPLRKCAELLGTEAEAGRRTPVGPGAGRGALRGQAAEPASPPGAKESPEPAAENYTSQEAARPGPRTRAGAKSAGGWSSGGLAWAPGAGRTPTCWRGARGGRAGSGRRTSARVRGRCGLRAARAPQDRRAAARPPFGPHCIRGKRLRGDVPLLPALPALPGCHRDIASPLHGDRLPRPTCPPTATRGPDERRSGRATSASPGRTTTSVRKARPPAPEWPAPAVGTWSSERHWGRLTSLTPRVRSDWLRRLSVTLRAARARLLPWRSLAATPRASRPCCRPALPRVAGGPRGGPAGRGAGRKEPGDCVPHRPGAGGRARTVGPRLRRRASGPCGSRAQRDREAGAPTLWCRPEPQARGPSFRSAEALGAPSPRKRGRTGDGMAPPSGPGPAAVLLAVQATGGSEWPLESVSYTVRGPSRHELQPPPGGPGALSLHFLDARDARRWAALVRRAAMEGRDGSLPQALGPDTCPVSPPSPPEVPTLQTPQPKVDLCWSPGDSPEKEELVGRLARAIEDGDEKGAAQAAALLAQHHVALSVRLREACFPPGPIRLQVTVEDAASSAHVSLQVHPHCTIATLQEQVFSEFGFPPAVQRWVIGQCLCVPERSLASYGVRRDGDPAFLYLLSAPGEASGRSPLGPQKTDGELSRLFPQSLGLPRAPQPASLPSPLQVGKGLGRVGLGLAGQLKDREAAAVSPPPQPGWSCPSCTFINAPSRPGCEMCSTQKPCSWDPLPAASTHQPPKVSKREDGLIPPGPRSLEPVLKLSGDFC

Target ORF information:

RefSeq Version	XM_005628075.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris SHANK-associated RH domain interactor (SHARPIN), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005628075.2

ORF Insert Sequence:

ATGCTAGGGT CCGGGGCTTC ATCTCCCGGG ATACGGCCGT CCCGCCGCAA GAGCATCACC 
CACCATGGGG GGTGGGGGGC GTCGCCGCGA GCCCGCGGGA TCCCTGCAGT AGCCCAGGGG 
CCGACTCCTG CGCCCGGTGA GGACCCGAAC ACCGAGGCGG GGGCCTGCGG TCGGTCACCT 
GGGCAGCGCT CTCCCCTGTC CTACGGGCTC CCCAGGGAGG CGCACAGGAA ACCCATAGGC 
TTCTCCGTTC CTCTCCGCAA GTGCGCCGAG CTCCTCGGAA CAGAGGCAGA GGCAGGCCGC 
AGGACCCCCG TAGGGCCCGG GGCCGGCCGC GGGGCGCTCC GGGGTCAGGC GGCCGAACCC 
GCCTCTCCGC CCGGGGCCAA GGAAAGCCCC GAGCCCGCCG CGGAGAACTA CACGTCCCAG 
GAGGCCGCGC GCCCCGGGCC GCGCACGCGC GCCGGCGCCA AGTCCGCTGG GGGCTGGAGT 
TCTGGAGGCC TGGCCTGGGC GCCCGGCGCC GGGCGGACAC CCACCTGCTG GCGCGGCGCT 
CGCGGGGGCC GGGCCGGGTC GGGCCGGCGG ACGTCGGCGC GGGTAAGAGG GCGCTGTGGG 
CTCCGGGCAG CGCGGGCTCC TCAGGACCGG AGGGCGGCGG CGCGGCCTCC GTTCGGGCCG 
CACTGTATCC GGGGGAAGAG GCTCCGCGGC GACGTCCCGT TACTCCCGGC CCTGCCGGCC 
CTGCCGGGCT GCCACCGCGA CATAGCCTCG CCCCTGCACG GCGACCGACT GCCGCGACCG 
ACCTGCCCGC CGACCGCCAC CCGAGGCCCG GACGAGCGCA GGAGCGGCCG AGCGACCTCT 
GCTTCCCCGG GCCGGACAAC AACAAGCGTC CGCAAGGCCC GGCCCCCCGC CCCTGAGTGG 
CCCGCTCCGG CCGTCGGGAC CTGGAGCTCG GAGCGCCATT GGGGACGGCT GACGTCGCTC 
ACTCCTCGCG TCCGCTCCGA CTGGCTGCGC CGCCTGTCCG TCACGCTCCG GGCCGCGCGA 
GCCCGCCTCC TCCCCTGGCG CTCATTGGCT GCCACCCCTC GCGCGTCCCG CCCCTGCTGT 
CGGCCCGCGC TCCCGAGAGT GGCTGGCGGC CCCCGGGGTG GGCCGGCGGG GCGGGGGGCG 
GGCCGGAAGG AGCCCGGGGA CTGCGTCCCG CACCGGCCGG GCGCGGGGGG GCGGGCCCGG 
ACCGTTGGAC CACGCCTCCG CCGCCGAGCC TCCGGGCCGT GTGGGTCCCG GGCCCAGCGG 
GACCGGGAGG CTGGCGCTCC GACCCTCTGG TGTCGGCCGG AACCCCAAGC GCGCGGCCCC 
AGTTTCCGGT CGGCCGAGGC GCTCGGAGCC CCGAGCCCAC GGAAGCGCGG CCGGACCGGA 
GATGGGATGG CGCCGCCCTC GGGCCCGGGC CCGGCCGCCG TGCTGCTGGC GGTGCAGGCC 
ACGGGCGGTT CGGAGTGGCC CTTGGAGTCC GTCTCCTACA CGGTCCGAGG CCCCAGCCGG 
CATGAGCTGC AGCCTCCGCC GGGAGGGCCC GGCGCCCTGA GCCTGCACTT CCTCGACGCC 
CGGGACGCTC GGCGCTGGGC CGCCCTGGTC CGACGGGCCG CCATGGAGGG ACGCGATGGC 
AGCCTGCCCC AAGCCCTGGG CCCAGACACA TGCCCTGTGT CCCCACCCAG CCCCCCCGAA 
GTGCCCACAC TCCAGACCCC ACAACCCAAG GTGGATCTTT GCTGGAGCCC TGGAGACTCT 
CCGGAGAAAG AGGAGCTGGT GGGGCGCCTG GCCCGGGCCA TCGAGGACGG AGATGAGAAG 
GGGGCAGCCC AGGCAGCAGC CCTGCTGGCC CAGCATCACG TGGCCCTCAG CGTCCGGCTC 
CGGGAGGCCT GCTTCCCTCC TGGCCCCATC AGGCTACAGG TCACAGTTGA AGATGCTGCG 
TCCTCTGCCC ACGTGTCCCT GCAAGTTCAC CCCCATTGCA CCATCGCAAC TCTCCAGGAG 
CAGGTGTTCT CGGAGTTTGG CTTCCCGCCA GCTGTGCAGC GCTGGGTCAT CGGGCAGTGC 
CTGTGTGTGC CCGAGCGCAG CCTTGCTTCG TATGGGGTCC GGCGGGATGG GGATCCTGCT 
TTCCTCTATC TGCTCTCAGC GCCCGGAGAG GCCTCAGGAC GCAGCCCTCT GGGCCCCCAG 
AAGACAGATG GGGAACTAAG TCGCCTGTTT CCGCAGTCAC TGGGGCTGCC CAGAGCGCCT 
CAGCCAGCCA GCCTCCCCAG CCCCCTTCAG GTGGGTAAGG GACTGGGCCG GGTGGGCCTG 
GGACTTGCTG GGCAGCTGAA GGATAGAGAA GCAGCCGCAG TCTCCCCGCC TCCCCAGCCT 
GGCTGGTCCT GCCCTTCCTG CACATTCATC AATGCCCCCA GCCGACCCGG CTGTGAGATG 
TGCAGCACCC AGAAGCCCTG CAGTTGGGAC CCCCTTCCTG CAGCCTCCAC CCACCAGCCG 
CCAAAGGTCT CGAAGAGAGA GGATGGCCTG ATCCCTCCGG GCCCGAGGTC CCTGGAGCCT 
GTCCTGAAAC TCTCAGGCGA CTTTTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SHARPIN ( XM_014118748.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013974223.1 Canis lupus familiaris SHANK-associated RH domain interactor (SHARPIN), transcript variant X1, mRNA.

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CloneID	OCe39772
Clone ID Related Accession (Same CDS sequence)	XM_014118748.1
Accession Version	XM_014118748.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2670bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	sharpin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726083.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAGGGT CCGGGGCTTC ATCTCCCGGG ATACGGCCGT CCCGCCGCAA GAGCATCACC 
CACCATGGGG GGTGGGGGGC GTCGCCGCGA GCCCGCGGGA TCCCTGCAGT AGCCCAGGGG 
CCGACTCCTG CGCCCGGTGA GGACCCGAAC ACCGAGGCGG GGGCCTGCGG TCGGTCACCT 
GGGCAGCGCT CTCCCCTGTC CTACGGGCTC CCCAGGGAGG CGCACAGGAA ACCCATAGGC 
TTCTCCGTTC CTCTCCGCAA GTGCGCCGAG CTCCTCGGAA CAGAGGCAGA GGCAGGCCGC 
AGGACCCCCG TAGGGCCCGG GGCCGGCCGC GGGGCGCTCC GGGGTCAGGC GGCCGAACCC 
GCCTCTCCGC CCGGGGCCAA GGAAAGCCCC GAGCCCGCCG CGGAGAACTA CACGTCCCAG 
GAGGCCGCGC GCCCCGGGCC GCGCACGCGC GCCGGCGCCA AGTCCGCTGG GGGCTGGAGT 
TCTGGAGGCC TGGCCTGGGC GCCCGGCGCC GGGCGGACAC CCACCTGCTG GCGCGGCGCT 
CGCGGGGGCC GGGCCGGGTC GGGCCGGCGG ACGTCGGCGC GGGTAAGAGG GCGCTGTGGG 
CTCCGGGCAG CGCGGGCTCC TCAGGACCGG AGGGCGGCGG CGCGGCCTCC GTTCGGGCCG 
CACTGTATCC GGGGGAAGAG GCTCCGCGGC GACGTCCCGT TACTCCCGGC CCTGCCGGCC 
CTGCCGGGCT GCCACCGCGA CATAGCCTCG CCCCTGCACG GCGACCGACT GCCGCGACCG 
ACCTGCCCGC CGACCGCCAC CCGAGGCCCG GACGAGCGCA GGAGCGGCCG AGCGACCTCT 
GCTTCCCCGG GCCGGACAAC AACAAGCGTC CGCAAGGCCC GGCCCCCCGC CCCTGAGTGG 
CCCGCTCCGG CCGTCGGGAC CTGGAGCTCG GAGCGCCATT GGGGACGGCT GACGTCGCTC 
ACTCCTCGCG TCCGCTCCGA CTGGCTGCGC CGCCTGTCCG TCACGCTCCG GGCCGCGCGA 
GCCCGCCTCC TCCCCTGGCG CTCATTGGCT GCCACCCCTC GCGCGTCCCG CCCCTGCTGT 
CGGCCCGCGC TCCCGAGAGT GGCTGGCGGC CCCCGGGGTG GGCCGGCGGG GCGGGGGGCG 
GGCCGGAAGG AGCCCGGGGA CTGCGTCCCG CACCGGCCGG GCGCGGGGGG GCGGGCCCGG 
ACCGTTGGAC CACGCCTCCG CCGCCGAGCC TCCGGGCCGT GTGGGTCCCG GGCCCAGCGG 
GACCGGGAGG CTGGCGCTCC GACCCTCTGG TGTCGGCCGG AACCCCAAGC GCGCGGCCCC 
AGTTTCCGGT CGGCCGAGGC GCTCGGAGCC CCGAGCCCAC GGAAGCGCGG CCGGACCGGA 
GATGGGATGG CGCCGCCCTC GGGCCCGGGC CCGGCCGCCG TGCTGCTGGC GGTGCAGGCC 
ACGGTGAGGC CGCTGGGCGC GGGCCCGGAC GCCGAGGCGC AGCTGCGGAG GCTGCAGCTG 
AGCGCGGACC CAGAGCGGCC CGGGCGTCTC CGGCTGCAGC TGCTGGGCGC GGGCCCCGGG 
GCGGGCGGTT CGGAGTGGCC CTTGGAGTCC GTCTCCTACA CGGTCCGAGG CCCCAGCCGG 
CATGAGCTGC AGCCTCCGCC GGGAGGGCCC GGCGCCCTGA GCCTGCACTT CCTCGACGCC 
CGGGACGCTC GGCGCTGGGC CGCCCTGGTC CGACGGGCCG CCATGGAGGG ACGCGATGGC 
AGCCTGCCCC AAGCCCTGGG CCCAGACACA TGCCCTGTGT CCCCACCCAG CCCCCCCGAA 
GTGCCCACAC TCCAGACCCC ACAACCCAAG GTGGATCTTT GCTGGAGCCC TGGAGACTCT 
CCGGAGAAAG AGGAGCTGGT GGGGCGCCTG GCCCGGGCCA TCGAGGACGG AGATGAGAAG 
GGGGCAGCCC AGGCAGCAGC CCTGCTGGCC CAGCATCACG TGGCCCTCAG CGTCCGGCTC 
CGGGAGGCCT GCTTCCCTCC TGGCCCCATC AGGCTACAGG TCACAGTTGA AGATGCTGCG 
TCCTCTGCCC ACGTGTCCCT GCAAGTTCAC CCCCATTGCA CCATCGCAAC TCTCCAGGAG 
CAGGTGTTCT CGGAGTTTGG CTTCCCGCCA GCTGTGCAGC GCTGGGTCAT CGGGCAGTGC 
CTGTGTGTGC CCGAGCGCAG CCTTGCTTCG TATGGGGTCC GGCGGGATGG GGATCCTGCT 
TTCCTCTATC TGCTCTCAGC GCCCGGAGAG GCCTCAGGAC GCAGCCCTCT GGGCCCCCAG 
AAGACAGATG GGGAACTAAG TCGCCTGTTT CCGCAGTCAC TGGGGCTGCC CAGAGCGCCT 
CAGCCAGCCA GCCTCCCCAG CCCCCTTCAG GTGGGTAAGG GACTGGGCCG GGTGGGCCTG 
GGACTTGCTG GGCAGCTGAA GGATAGAGAA GCAGCCGCAG TCTCCCCGCC TCCCCAGCCT 
GGCTGGTCCT GCCCTTCCTG CACATTCATC AATGCCCCCA GCCGACCCGG CTGTGAGATG 
TGCAGCACCC AGAAGCCCTG CAGTTGGGAC CCCCTTCCTG CAGCCTCCAC CCACCAGCCG 
CCAAAGGTCT CGAAGAGAGA GGATGGCCTG ATCCCTCCGG GCCCGAGGTC CCTGGAGCCT 
GTCCTGAAAC TCTCAGGCGA CTTTTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013974223.1
CDS	265..2934
Translation	MLGSGASSPGIRPSRRKSITHHGGWGASPRARGIPAVAQGPTPAPGEDPNTEAGACGRSPGQRSPLSYGLPREAHRKPIGFSVPLRKCAELLGTEAEAGRRTPVGPGAGRGALRGQAAEPASPPGAKESPEPAAENYTSQEAARPGPRTRAGAKSAGGWSSGGLAWAPGAGRTPTCWRGARGGRAGSGRRTSARVRGRCGLRAARAPQDRRAAARPPFGPHCIRGKRLRGDVPLLPALPALPGCHRDIASPLHGDRLPRPTCPPTATRGPDERRSGRATSASPGRTTTSVRKARPPAPEWPAPAVGTWSSERHWGRLTSLTPRVRSDWLRRLSVTLRAARARLLPWRSLAATPRASRPCCRPALPRVAGGPRGGPAGRGAGRKEPGDCVPHRPGAGGRARTVGPRLRRRASGPCGSRAQRDREAGAPTLWCRPEPQARGPSFRSAEALGAPSPRKRGRTGDGMAPPSGPGPAAVLLAVQATVRPLGAGPDAEAQLRRLQLSADPERPGRLRLQLLGAGPGAGGSEWPLESVSYTVRGPSRHELQPPPGGPGALSLHFLDARDARRWAALVRRAAMEGRDGSLPQALGPDTCPVSPPSPPEVPTLQTPQPKVDLCWSPGDSPEKEELVGRLARAIEDGDEKGAAQAAALLAQHHVALSVRLREACFPPGPIRLQVTVEDAASSAHVSLQVHPHCTIATLQEQVFSEFGFPPAVQRWVIGQCLCVPERSLASYGVRRDGDPAFLYLLSAPGEASGRSPLGPQKTDGELSRLFPQSLGLPRAPQPASLPSPLQVGKGLGRVGLGLAGQLKDREAAAVSPPPQPGWSCPSCTFINAPSRPGCEMCSTQKPCSWDPLPAASTHQPPKVSKREDGLIPPGPRSLEPVLKLSGDFC

Target ORF information:

RefSeq Version	XM_014118748.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris SHANK-associated RH domain interactor (SHARPIN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014118748.1

ORF Insert Sequence:

ATGCTAGGGT CCGGGGCTTC ATCTCCCGGG ATACGGCCGT CCCGCCGCAA GAGCATCACC 
CACCATGGGG GGTGGGGGGC GTCGCCGCGA GCCCGCGGGA TCCCTGCAGT AGCCCAGGGG 
CCGACTCCTG CGCCCGGTGA GGACCCGAAC ACCGAGGCGG GGGCCTGCGG TCGGTCACCT 
GGGCAGCGCT CTCCCCTGTC CTACGGGCTC CCCAGGGAGG CGCACAGGAA ACCCATAGGC 
TTCTCCGTTC CTCTCCGCAA GTGCGCCGAG CTCCTCGGAA CAGAGGCAGA GGCAGGCCGC 
AGGACCCCCG TAGGGCCCGG GGCCGGCCGC GGGGCGCTCC GGGGTCAGGC GGCCGAACCC 
GCCTCTCCGC CCGGGGCCAA GGAAAGCCCC GAGCCCGCCG CGGAGAACTA CACGTCCCAG 
GAGGCCGCGC GCCCCGGGCC GCGCACGCGC GCCGGCGCCA AGTCCGCTGG GGGCTGGAGT 
TCTGGAGGCC TGGCCTGGGC GCCCGGCGCC GGGCGGACAC CCACCTGCTG GCGCGGCGCT 
CGCGGGGGCC GGGCCGGGTC GGGCCGGCGG ACGTCGGCGC GGGTAAGAGG GCGCTGTGGG 
CTCCGGGCAG CGCGGGCTCC TCAGGACCGG AGGGCGGCGG CGCGGCCTCC GTTCGGGCCG 
CACTGTATCC GGGGGAAGAG GCTCCGCGGC GACGTCCCGT TACTCCCGGC CCTGCCGGCC 
CTGCCGGGCT GCCACCGCGA CATAGCCTCG CCCCTGCACG GCGACCGACT GCCGCGACCG 
ACCTGCCCGC CGACCGCCAC CCGAGGCCCG GACGAGCGCA GGAGCGGCCG AGCGACCTCT 
GCTTCCCCGG GCCGGACAAC AACAAGCGTC CGCAAGGCCC GGCCCCCCGC CCCTGAGTGG 
CCCGCTCCGG CCGTCGGGAC CTGGAGCTCG GAGCGCCATT GGGGACGGCT GACGTCGCTC 
ACTCCTCGCG TCCGCTCCGA CTGGCTGCGC CGCCTGTCCG TCACGCTCCG GGCCGCGCGA 
GCCCGCCTCC TCCCCTGGCG CTCATTGGCT GCCACCCCTC GCGCGTCCCG CCCCTGCTGT 
CGGCCCGCGC TCCCGAGAGT GGCTGGCGGC CCCCGGGGTG GGCCGGCGGG GCGGGGGGCG 
GGCCGGAAGG AGCCCGGGGA CTGCGTCCCG CACCGGCCGG GCGCGGGGGG GCGGGCCCGG 
ACCGTTGGAC CACGCCTCCG CCGCCGAGCC TCCGGGCCGT GTGGGTCCCG GGCCCAGCGG 
GACCGGGAGG CTGGCGCTCC GACCCTCTGG TGTCGGCCGG AACCCCAAGC GCGCGGCCCC 
AGTTTCCGGT CGGCCGAGGC GCTCGGAGCC CCGAGCCCAC GGAAGCGCGG CCGGACCGGA 
GATGGGATGG CGCCGCCCTC GGGCCCGGGC CCGGCCGCCG TGCTGCTGGC GGTGCAGGCC 
ACGGTGAGGC CGCTGGGCGC GGGCCCGGAC GCCGAGGCGC AGCTGCGGAG GCTGCAGCTG 
AGCGCGGACC CAGAGCGGCC CGGGCGTCTC CGGCTGCAGC TGCTGGGCGC GGGCCCCGGG 
GCGGGCGGTT CGGAGTGGCC CTTGGAGTCC GTCTCCTACA CGGTCCGAGG CCCCAGCCGG 
CATGAGCTGC AGCCTCCGCC GGGAGGGCCC GGCGCCCTGA GCCTGCACTT CCTCGACGCC 
CGGGACGCTC GGCGCTGGGC CGCCCTGGTC CGACGGGCCG CCATGGAGGG ACGCGATGGC 
AGCCTGCCCC AAGCCCTGGG CCCAGACACA TGCCCTGTGT CCCCACCCAG CCCCCCCGAA 
GTGCCCACAC TCCAGACCCC ACAACCCAAG GTGGATCTTT GCTGGAGCCC TGGAGACTCT 
CCGGAGAAAG AGGAGCTGGT GGGGCGCCTG GCCCGGGCCA TCGAGGACGG AGATGAGAAG 
GGGGCAGCCC AGGCAGCAGC CCTGCTGGCC CAGCATCACG TGGCCCTCAG CGTCCGGCTC 
CGGGAGGCCT GCTTCCCTCC TGGCCCCATC AGGCTACAGG TCACAGTTGA AGATGCTGCG 
TCCTCTGCCC ACGTGTCCCT GCAAGTTCAC CCCCATTGCA CCATCGCAAC TCTCCAGGAG 
CAGGTGTTCT CGGAGTTTGG CTTCCCGCCA GCTGTGCAGC GCTGGGTCAT CGGGCAGTGC 
CTGTGTGTGC CCGAGCGCAG CCTTGCTTCG TATGGGGTCC GGCGGGATGG GGATCCTGCT 
TTCCTCTATC TGCTCTCAGC GCCCGGAGAG GCCTCAGGAC GCAGCCCTCT GGGCCCCCAG 
AAGACAGATG GGGAACTAAG TCGCCTGTTT CCGCAGTCAC TGGGGCTGCC CAGAGCGCCT 
CAGCCAGCCA GCCTCCCCAG CCCCCTTCAG GTGGGTAAGG GACTGGGCCG GGTGGGCCTG 
GGACTTGCTG GGCAGCTGAA GGATAGAGAA GCAGCCGCAG TCTCCCCGCC TCCCCAGCCT 
GGCTGGTCCT GCCCTTCCTG CACATTCATC AATGCCCCCA GCCGACCCGG CTGTGAGATG 
TGCAGCACCC AGAAGCCCTG CAGTTGGGAC CCCCTTCCTG CAGCCTCCAC CCACCAGCCG 
CCAAAGGTCT CGAAGAGAGA GGATGGCCTG ATCCCTCCGG GCCCGAGGTC CCTGGAGCCT 
GTCCTGAAAC TCTCAGGCGA CTTTTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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SHARPIN cDNA ORF clone, Canis lupus familiaris(Dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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