F8 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	F8
Entrez Gene ID	403875
Full Name	coagulation factor VIII
General protein information	Preferred Names coagulation factor VIII Names coagulation factor VIII coagulation co-factor coagulation factor VIII, procoagulant component (hemophilia A) factor VIII procoagulant component
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001003212.1	NP_001003212.1	coagulation factor VIII precursor
XM_005640988.2	XP_005641045.1	coagulation factor VIII isoform X1
XM_022415494.1	XP_022271202.1	coagulation factor VIII isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	F8	NP_000123.1
Canis lupus familiaris (dog)	F8	NP_001003212.1
Pan troglodytes (chimpanzee)	F8	XP_003317837.1
Rattus norvegicus (Norway rat)	F8	NP_899160.1
Macaca mulatta (Rhesus monkey)	LOC100424151	XP_002806506.1
Mus musculus (house mouse)	F8	NP_032003.2
Bos taurus (cattle)	F8	NP_001138980.1

Gene Ontology
Bibliography

Related articles in PubMed

Omental implantation of BOECs in hemophilia dogs results in circulating FVIII antigen and a complex immune response.
Ozelo MC, Vidal B, Brown C, Notley C, Hegadorn C, Webster S, Harpell L, Ahlin J, Winterborn A, Handforth J, Arruda VR, Hough C, Lillicrap D
Blood123(26)4045-53(2014 Jun)

Minimal modification in the factor VIII B-domain sequence ameliorates the murine hemophilia A phenotype.
Siner JI, Iacobelli NP, Sabatino DE, Ivanciu L, Zhou S, Poncz M, Camire RM, Arruda VR
Blood121(21)4396-403(2013 May)

The canine factor VIII cDNA and 5' flanking sequence.
Cameron C, Notley C, Hoyle S, McGlynn L, Hough C, Kamisue S, Giles A, Lillicrap D
Thrombosis and haemostasis79(2)317-22(1998 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following F8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the F8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe34078	NM_001003212.1 Latest version!	Canis lupus familiaris coagulation factor VIII (F8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OCe31938	XM_005640988.2 Latest version!	Canis lupus familiaris coagulation factor VIII, procoagulant component (F8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe31938	XM_022415494.1 Latest version!	Canis lupus familiaris coagulation factor VIII (F8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

F8 ( NM_001003212.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001003212.1 Canis lupus familiaris coagulation factor VIII (F8), mRNA.

Price & Availability↑

CloneID	OCe34078
Clone ID Related Accession (Same CDS sequence)	NM_001003212.1
Accession Version	NM_001003212.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7032bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550160000000
Organism	Canis lupus familiaris(dog)
Product	coagulation factor VIII precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF049489.1. ##Evidence-Data-START## Transcript exon combination :: AF049489.1, AF016234.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA103936001, SAMEA103936002 [ECO:0000348] ##Evidence-Data-END##

ATGCAAGTAG AGCTCTACAC CTGCTGCTTT CTGTGCCTTT TGCCCTTCAG CCTTAGTGCC 
ACCAGAAAAT ACTACCTCGG TGCAGTGGAA CTGTCCTGGG ACTATATGCA AAGTGACCTG 
CTCAGTGCGC TGCACGCGGA TACAAGCTTT TCTTCCAGGG TGCCAGGATC TTTGCCACTC 
ACCACGTCAG TCACGTACAG AAAGACTGTG TTTGTAGAGT TTACAGATGA CCTTTTCAAC 
ATTGCCAAGC CCAGGCCACC GTGGATGGGC CTGCTGGGTC CTACCATCCA GGCTGAGGTT 
TATGACACAG TGGTCATTGT CCTTAAGAAC ATGGCTTCTC ATCCTGTCAG CCTTCACGCT 
GTTGGTGTAT CCTATTGGAA AGCTTCTGAA GGTGCTGAGT ATGAGGATCA GACCAGCCAA 
AAGGAGAAGG AAGATGATAA TGTCATTCCT GGTGAAAGCC ATACCTATGT CTGGCAGGTC 
CTGAAAGAGA ATGGCCCAAT GGCCTCTGAT CCACCATGTC TCACCTACTC ATATTTTTCA 
CACGTGGACC TGGTGAAAGA CCTGAATTCA GGCCTCATTG GAGCCCTGCT GGTTTGCAAA 
GAAGGGAGTC TGGCCAAAGA AAGGACACAG ACCTTGCAGG AATTTGTCCT ACTTTTTGCT 
GTATTTGATG AAGGGAAAAG TTGGCACTCA GAAACAAATG CGTCTTTGAC ACAGGCTGAG 
GCCCAGCATG AGCTGCACAC CATCAATGGC TATGTAAACA GGTCTCTGCC AGGTCTTACT 
GTGTGTCACA AGAGATCAGT CTATTGGCAT GTGATTGGAA TGGGCACCAC CCCCGAAGTG 
CACTCAATTT TTCTCGAAGG TCACACATTT CTTGTGGGGA ACCACCGCCA GGCCTCCTTG 
GAGATCTCGC CAATTACTTT CCTTACTGCT CAGACATTCC TGATGGACCT TGGCCAGTTT 
CTACTGTTTT GTCATATCCC TTCCCATCAA CATGATGGTA TGGAAGCTTA TGTCAAAGTA 
GATAGCTGCC CAGAGGAACC CCAGCTGCGC ATGAAAAATA ATGAAGATAA AGATTATGAT 
GATGGTCTTT ATGGTTCTGA CATGGACGTA GTTAGCTTTG ATGACGACAG CTCTTCTCCC 
TTTATCCAAA TCCGCTCAGT TGCCAAGAAG CATCCTAAAA CTTGGGTCCA CTATATTGCT 
GCTGAGGAGG AGGACTGGGA CTATGCTCCC TCAGGCCCCA CCCCCAATGA TAGAAGTCAT 
AAAAATCTGT ATTTGAACAA TGGTCCTCAG CGGATTGGTA AGAAGTACAA AAAAGTCCGA 
TTTGTGGCAT ACACAGATGA GACATTTAAG ACTCGTGAAG CTATTCAGTA TGAATCAGGA 
ATCCTGGGAC CTTTACTTTA TGGAGAAGTT GGAGACACAC TGCTGATTAT ATTTAAGAAA 
CAAGCCAGCC GGCCATATAA CATCTACCCT CATGGGATCA ATTATGTCAC TCCTCTGCAC 
ACAGGGAGAT TGCCAAAAGG TGTGAAACAT TTGAAAGATA TGCCAATTCT GCCGGGAGAG 
ATATTCAAGT ATAAATGGAC AGTGACCGTA GAAGATGGAC CAACTAAATC AGATCCTCGG 
TGCCTGACCC GATATTACTC AAGCTTCATT AATCTGGAGA GAGATCTAGC TTCAGGACTC 
ATTGGCCCTC TTCTCATCTG CTACAAAGAA TCTGTAGATC AAAGAGGAAA CCAGATGATG 
TCAGACAAGA GAAATGTCAT CCTGTTTTCT GTACTTGATG AGAATCGAAG CTGGTACCTC 
ACAGAGGATA TGCAGCGCTT CCTCCCCAAT GCAGATGTAG TGCAGCCCCA TGACCCAGAG 
TTCCAACTCT CTAACATCAT GCACAGCATC AATGGCTATG TTTTTGACAA CTTGCAGCTG 
TCAGTTTGTT TGCATGAGGT GGCGTACTGG TACATTCTAA GTGTTGGAGC ACAAACTGAC 
TTCCTGTCTG TCTTCTTCTC TGGATATACC TTCAAACACA AAATGGTCTA TGAAGACACA 
CTTACCCTCT TCCCATTCTC AGGAGAAACT GTCTTCATGT CAATGGAAAA CCCAGGTCTG 
TGGGTTCTGG GGTGCCACAA CTCAGACTTT CGGAACAGAG GCATGACAGC CTTACTGAAG 
GTTTCTAGTT GTAACAGGAA CATTGATGAT TATTATGAGG ACACATACGA AGATATTCCA 
ACTCCCCTGC TAAATGAAAA CAATGTAATT AAACCTAGAA GCTTCTCCCA GAATTCAAGG 
CACCCTAGCA CTAAGGAAAA GCAATTGAAA GCCACCACAA CTCCAGAAAA TGACATAGAG 
AAGATTGACC TTCAATCTGG AGAAAGAACA CAGCTGATTA AAGCACAAAG TGTCTCCTCT 
AGTGATTTGT TGATGCTGTT GGGACAGAAT CCTACTCCAC GTGGACTGTT CTTATCTGAT 
CTCCGAGAGG CCACAGATAG AGCCGATGAC CATTCACGTG GAGCAATAGA AAGAAACAAG 
GGCCCACCTG AAGTGGCAAG TCTCAGACCA GAGCTCCGTC ACAGTGAGGA CAGAGAATTT 
ACTCCTGAGC CAGAACTGCA GTTAAGATTA AATGAGAATT TGGGGACAAA TACAACAGTA 
GAGTTGAAGA AACTTGATTT AAAAATTTCT AGTTCATCAG ACAGTCTAAT GACTTCACCA 
ACAATTCCAT CAGATAAGTT GGCAGCAGCT ACTGAAAAGA CAGGTTCCTT AGGACCCCCA 
AATATGTCAG TTCACTTTAA CGGTCATTTA GGTACCATTG TATTTGGCAA TAATTCATCC 
CACCTTATTC AGTCTGGTGT ACCTTTGGAA TTGAGTGAAG AAGATAACGA TTCCAAGTTG 
TTAGAAGCAC CTTTAATGAA TATTCAAGAA AGTTCACTGA GAGAAAATGT ATTATCAATG 
GAAAGTAATA GGTTATTTAA AGAAGAAAGA ATTCGTGGAC CTGCTTCATT AATCAAAGAT 
AATGCTTTAT TCAAAGTTAA TATCTCTTCG GTAAAGACAA ACAGGGCACC AGTTAACTTA 
ACAACTAATA GAAAGACTCG TGTTGCTATC CCAACATTAT TAATTGAGAA CAGTACCTCA 
GTCTGGCAAG ATATTATGTT AGAAAGGAAT ACTGAGTTTA AAGAAGTAAC TTCTTTGATT 
CATAATGAAA CGTTTATGGA CAGAAATACT ACAGCTCTGG GGCTAAATCA TGTGTCAAAT 
AAAACTACTT TATCAAAAAA TGTGGAAATG GCCCACCAAA AAAAAGAAGA CCCTGTGCCA 
CTACGTGCAG AAAATCCAGA TCTATCATCC TCCAAGATAC CGTTCTTGCC AGATTGGATA 
AAGACCCATG GCAAGAACTC CCTAAGCTCT GAGCAAAGGC CCAGTCCAAA ACAATTAACA 
TCTTTAGGAT CAGAAAAATC TGTGAAAGAT CAGAACTTTT TGTCAGAGGA GAAGGTGGTA 
GTAGGAGAGG ATGAATTTAC GAAGGACACA GAACTCCAAG AGATTTTTCC AAACAACAAG 
AGCATATTTT TTGCTAACTT GGCTAATGTC CAAGAAAATG ATACATACAA TCAAGAAAAA 
AAATCTCTGG AAGAGATAGA AAGAAAGGAA AAATTAACCC AGGAGAATGT GGCTTTGCCT 
CAGGCACATA CTATGATTGG CACTAAGAAC TTCCTGAAGA ACCTTTTCTT ACTAAGCACT 
AAGCAAAATG TAGCAGGTTT AGAAGAGCAG CCATATACTC CAATACTTCA AGACACCAGG 
TCATTAAATG ATTCGCCACA TAGTGAAGGG ATTCATATGG CCAATTTCTC AAAAATAAGG 
GAAGAAGCAA ACTTGGAAGG CTTGGGAAAT CAAACAAACC AAATGGTAGA GAGGTTTCCA 
AGCACTACGA GGATGTCTTC TAATGCAAGT CAGCATGTTA TCACTCAACG TGGTAAGCGG 
AGTTTGAAAC AACCCAGACT CTCACAAGGA GAAATAAAGT TTGAAAGGAA GGTTATTGCA 
AATGACACTT CAACCCAGTG GTCCAAAAAC ATGAACTATT TGGCCCAGGG AACCCTCACA 
CAGATAGAGT ATAATGAGAA AGAAAAAAGG GCCATTACTC AGTCCCCCCT ATCAGATTGT 
TCTATGAGGA ATCATGTCAC CATTCAAATG AATGACTCTG CATTACCCGT TGCAAAGGAA 
TCAGCATCTC CATCAGTTAG ACATACAGAT CTGACCAAGA TCCCATCCCA ACACAACTCT 
TCTCATCTTC CAGCATCGGC CTGTAATTAT ACCTTTAGAG AGAGGACTTC TGGAGTCCAA 
GAAGGCAGTC ATTTCTTACA AGAAGCCAAA AGAAATAACC TCTCTTTAGC CTTTGTAACC 
TTAGGAATAA CTGAAGGGCA AGGAAAGTTC AGCTCCCTGG GGAAAAGTGC CACAAACCAA 
CCCATGTACA AGAAACTTGA AAACACTGTT CTCTTGCAAC CAGGCTTGTC CGAAACATCT 
GACAAAGTTG AATTACTTTC TCAAGTTCAT GTTGATCAAG AAGACTCTTT CCCTACAAAA 
ACTAGCAATG ATTCTCCTGG CCACCTGGAT CTCATGGGAA AGATCTTCCT TCAGAAAACA 
CAGGGACCTG TTAAAATGAA TAAAACAAAT AGCCCTGGAA AAGTGCCCTT TCTGAAATGG 
GCAACAGAAA GCTCTGAAAA GATTCCCTCC AAGCTGCTGG GTGTCCTTGC TTGGGATAAC 
CACTATGATA CCCAGATACC AAGTGAAGAG TGGAAATCCC AAAAAAAGTC ACAGACGAAC 
ACAGCTTTTA AAAGGAAAGA CACCATTTTG CCCCTGGGCC CTTGTGAAAA TAATGATTCA 
ACAGCAGCAA TAAATGAAGG ACAAGATAAG CCCCAAAGAG AAGCTATGTG GGCAAAGCAA 
GGAGAGCCTG GAAGGTTGTG CTCTCAAAAC CCACCAGTCT CAAAACACCA TCAAAGGGAA 
ATAACCGTTA CTACTCTTCA GCCAGAGGAA GACAAATTTG AGTATGATGA CACCTTCTCA 
ATTGAAATGA AGAGAGAAGA TTTTGACATC TACGGCGACT ATGAAGATCA GGGCCTCCGC 
AGCTTTCAAA AGAAAACACG ACACTATTTC ATTGCTGCAG TGGAGCGTCT CTGGGATTAT 
GGGATGAGTA GATCTCCCCA TATACTAAGA AACAGGGCTC AAAGTGGGGA TGTCCAGCAG 
TTCAAGAAGG TGGTTTTCCA GGAATTTACT GATGGATCCT TTACTCAGCC CTTATACCGT 
GGAGAACTGA ATGAACACTT GGGACTCTTG GGGCCATATA TAAGAGCAGA AGTTGAAGAC 
AATATCGTGG TAACTTTCAA AAACCAGGCC TCTCGTCCCT ACTCCTTCTA TTCTAGTCTT 
ATTTCTTATG ACGAAGATGA GGGACAAGGA GCAGAACCTA GAAGAAAGTT TGTCAACCCT 
AATGAAACCA AAATTTACTT TTGGAAAGTA CAGCATCATA TGGCACCCAC TAAAGATGAG 
TTTGACTGCA AAGCCTGGGC TTATTTTTCT GATGTTGATC TGGAGAAAGA TGTGCACTCA 
GGCTTGATTG GACCCCTTCT GATCTGCCGC AGTAACACAC TGAACCCTGC TCATGGGAGA 
CAAGTGACAG TGCAGGAGTT TGCCCTGGTT TTCACTATAT TCGATGAGAC TAAGAGCTGG 
TACTTCACTG AAAACCTGGA AAGGAACTGC AGAGCTCCCT GCAATGTCCA GAAGGAGGAC 
CCTACTCTAA AAGAAAACTT CCGCTTCCAT GCAATCAACG GCTATGTGAA GGATACACTC 
CCTGGCTTAG TAATGGCTCA GGATCAAAAG GTTCGATGGT ATCTGCTCAG CATGGGCAGC 
AACGAAAACA TTCATTCCAT TCACTTCAGT GGACATGTGT TCACTGTACG GAAAAAAGAG 
GAATATAAAA TGGCAGTCTA CAACCTCTAT CCAGGTGTTT TTGAGACTGT GGAAATGCTA 
CCATCCCAAG TTGGAATCTG GCGGATAGAA TGCCTTATCG GCGAGCACCT GCAAGCCGGG 
ATGAGCACTC TGTTTCTGGT GTACAGCAAG AAGTGTCAGA CTCCACTGGG GATGGCTTCC 
GGACACATTA GAGATTTTCA GATTACAGCT TCAGGACAAT ATGGACAGTG GGCCCCAAAG 
CTGGCCAGAC TTCATTATTC CGGATCAATC AATGCCTGGA GCACCAAGGA TCCCTTTTCC 
TGGATCAAGG TGGATCTCTT GGCACCGATG ATTATTCACG GCATCATGAC CCAGGGGGCC 
CGCCAGAAGT TCTCCAGCCT CTACGTGTCT CAGTTTATCA TCATGTACAG TCTGGATGGC 
AACAAGTGGC ACAGTTACCG AGGGAATTCC ACGGGGACCT TAATGGTCTT CTTTGGCAAC 
GTGGATTCAT CTGGGATCAA ACACAATATT TTTAACCCTC CGATTATTGC TCAGTACATC 
CGTTTGCACC CAACCCATTA CAGCATCCGC AGCACTCTTC GCATGGAGCT CTTGGGCTGT 
GACTTCAACA GTTGCAGCAT GCCGCTGGGG ATGGAGAGTA AAGCAATATC AGATGCTCAG 
ATCACTGCCT CGTCCTACCT AAGCAGTATG CTTGCCACTT GGTCTCCTTC CCAAGCCCGG 
CTGCACCTGC AGGGCAGGAC TAATGCCTGG AGACCTCAGG CAAATAACCC AAAAGAGTGG 
CTGCAAGTGG ACTTCCGGAA GACCATGAAA GTCACAGGAA TAACCACCCA GGGGGTGAAA 
TCTCTCCTCA TCAGCATGTA TGTGAAGGAG TTCCTCATCT CCAGTAGTCA AGATGGCCAT 
AACTGGACTC TGTTTCTTCA GAATGACAAA GTCAAGGTCT TCCAGGGAAA CCGGGACTCC 
TCCACGCCTG TGCGGAACGC TCTCGAACCC CCGCTGGTGG CTCGCTACGT GCGCCTGCAC 
CCGCAGAGCT GGGCGCACCA CATCGCCCTG AGGCTGGAGG TCCTGGGCTG CGACACCCAG 
CAGCCCGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003212.1
CDS	1..7032
Translation	MQVELYTCCFLCLLPFSLSATRKYYLGAVELSWDYMQSDLLSALHADTSFSSRVPGSLPLTTSVTYRKTVFVEFTDDLFNIAKPRPPWMGLLGPTIQAEVYDTVVIVLKNMASHPVSLHAVGVSYWKASEGAEYEDQTSQKEKEDDNVIPGESHTYVWQVLKENGPMASDPPCLTYSYFSHVDLVKDLNSGLIGALLVCKEGSLAKERTQTLQEFVLLFAVFDEGKSWHSETNASLTQAEAQHELHTINGYVNRSLPGLTVCHKRSVYWHVIGMGTTPEVHSIFLEGHTFLVGNHRQASLEISPITFLTAQTFLMDLGQFLLFCHIPSHQHDGMEAYVKVDSCPEEPQLRMKNNEDKDYDDGLYGSDMDVVSFDDDSSSPFIQIRSVAKKHPKTWVHYIAAEEEDWDYAPSGPTPNDRSHKNLYLNNGPQRIGKKYKKVRFVAYTDETFKTREAIQYESGILGPLLYGEVGDTLLIIFKKQASRPYNIYPHGINYVTPLHTGRLPKGVKHLKDMPILPGEIFKYKWTVTVEDGPTKSDPRCLTRYYSSFINLERDLASGLIGPLLICYKESVDQRGNQMMSDKRNVILFSVLDENRSWYLTEDMQRFLPNADVVQPHDPEFQLSNIMHSINGYVFDNLQLSVCLHEVAYWYILSVGAQTDFLSVFFSGYTFKHKMVYEDTLTLFPFSGETVFMSMENPGLWVLGCHNSDFRNRGMTALLKVSSCNRNIDDYYEDTYEDIPTPLLNENNVIKPRSFSQNSRHPSTKEKQLKATTTPENDIEKIDLQSGERTQLIKAQSVSSSDLLMLLGQNPTPRGLFLSDLREATDRADDHSRGAIERNKGPPEVASLRPELRHSEDREFTPEPELQLRLNENLGTNTTVELKKLDLKISSSSDSLMTSPTIPSDKLAAATEKTGSLGPPNMSVHFNGHLGTIVFGNNSSHLIQSGVPLELSEEDNDSKLLEAPLMNIQESSLRENVLSMESNRLFKEERIRGPASLIKDNALFKVNISSVKTNRAPVNLTTNRKTRVAIPTLLIENSTSVWQDIMLERNTEFKEVTSLIHNETFMDRNTTALGLNHVSNKTTLSKNVEMAHQKKEDPVPLRAENPDLSSSKIPFLPDWIKTHGKNSLSSEQRPSPKQLTSLGSEKSVKDQNFLSEEKVVVGEDEFTKDTELQEIFPNNKSIFFANLANVQENDTYNQEKKSLEEIERKEKLTQENVALPQAHTMIGTKNFLKNLFLLSTKQNVAGLEEQPYTPILQDTRSLNDSPHSEGIHMANFSKIREEANLEGLGNQTNQMVERFPSTTRMSSNASQHVITQRGKRSLKQPRLSQGEIKFERKVIANDTSTQWSKNMNYLAQGTLTQIEYNEKEKRAITQSPLSDCSMRNHVTIQMNDSALPVAKESASPSVRHTDLTKIPSQHNSSHLPASACNYTFRERTSGVQEGSHFLQEAKRNNLSLAFVTLGITEGQGKFSSLGKSATNQPMYKKLENTVLLQPGLSETSDKVELLSQVHVDQEDSFPTKTSNDSPGHLDLMGKIFLQKTQGPVKMNKTNSPGKVPFLKWATESSEKIPSKLLGVLAWDNHYDTQIPSEEWKSQKKSQTNTAFKRKDTILPLGPCENNDSTAAINEGQDKPQREAMWAKQGEPGRLCSQNPPVSKHHQREITVTTLQPEEDKFEYDDTFSIEMKREDFDIYGDYEDQGLRSFQKKTRHYFIAAVERLWDYGMSRSPHILRNRAQSGDVQQFKKVVFQEFTDGSFTQPLYRGELNEHLGLLGPYIRAEVEDNIVVTFKNQASRPYSFYSSLISYDEDEGQGAEPRRKFVNPNETKIYFWKVQHHMAPTKDEFDCKAWAYFSDVDLEKDVHSGLIGPLLICRSNTLNPAHGRQVTVQEFALVFTIFDETKSWYFTENLERNCRAPCNVQKEDPTLKENFRFHAINGYVKDTLPGLVMAQDQKVRWYLLSMGSNENIHSIHFSGHVFTVRKKEEYKMAVYNLYPGVFETVEMLPSQVGIWRIECLIGEHLQAGMSTLFLVYSKKCQTPLGMASGHIRDFQITASGQYGQWAPKLARLHYSGSINAWSTKDPFSWIKVDLLAPMIIHGIMTQGARQKFSSLYVSQFIIMYSLDGNKWHSYRGNSTGTLMVFFGNVDSSGIKHNIFNPPIIAQYIRLHPTHYSIRSTLRMELLGCDFNSCSMPLGMESKAISDAQITASSYLSSMLATWSPSQARLHLQGRTNAWRPQANNPKEWLQVDFRKTMKVTGITTQGVKSLLISMYVKEFLISSSQDGHNWTLFLQNDKVKVFQGNRDSSTPVRNALEPPLVARYVRLHPQSWAHHIALRLEVLGCDTQQPA

Target ORF information:

RefSeq Version	NM_001003212.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris coagulation factor VIII (F8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003212.1

ORF Insert Sequence:

ATGCAAGTAG AGCTCTACAC CTGCTGCTTT CTGTGCCTTT TGCCCTTCAG CCTTAGTGCC 
ACCAGAAAAT ACTACCTCGG TGCAGTGGAA CTGTCCTGGG ACTATATGCA AAGTGACCTG 
CTCAGTGCGC TGCACGCGGA TACAAGCTTT TCTTCCAGGG TGCCAGGATC TTTGCCACTC 
ACCACGTCAG TCACGTACAG AAAGACTGTG TTTGTAGAGT TTACAGATGA CCTTTTCAAC 
ATTGCCAAGC CCAGGCCACC GTGGATGGGC CTGCTGGGTC CTACCATCCA GGCTGAGGTT 
TATGACACAG TGGTCATTGT CCTTAAGAAC ATGGCTTCTC ATCCTGTCAG CCTTCACGCT 
GTTGGTGTAT CCTATTGGAA AGCTTCTGAA GGTGCTGAGT ATGAGGATCA GACCAGCCAA 
AAGGAGAAGG AAGATGATAA TGTCATTCCT GGTGAAAGCC ATACCTATGT CTGGCAGGTC 
CTGAAAGAGA ATGGCCCAAT GGCCTCTGAT CCACCATGTC TCACCTACTC ATATTTTTCA 
CACGTGGACC TGGTGAAAGA CCTGAATTCA GGCCTCATTG GAGCCCTGCT GGTTTGCAAA 
GAAGGGAGTC TGGCCAAAGA AAGGACACAG ACCTTGCAGG AATTTGTCCT ACTTTTTGCT 
GTATTTGATG AAGGGAAAAG TTGGCACTCA GAAACAAATG CGTCTTTGAC ACAGGCTGAG 
GCCCAGCATG AGCTGCACAC CATCAATGGC TATGTAAACA GGTCTCTGCC AGGTCTTACT 
GTGTGTCACA AGAGATCAGT CTATTGGCAT GTGATTGGAA TGGGCACCAC CCCCGAAGTG 
CACTCAATTT TTCTCGAAGG TCACACATTT CTTGTGGGGA ACCACCGCCA GGCCTCCTTG 
GAGATCTCGC CAATTACTTT CCTTACTGCT CAGACATTCC TGATGGACCT TGGCCAGTTT 
CTACTGTTTT GTCATATCCC TTCCCATCAA CATGATGGTA TGGAAGCTTA TGTCAAAGTA 
GATAGCTGCC CAGAGGAACC CCAGCTGCGC ATGAAAAATA ATGAAGATAA AGATTATGAT 
GATGGTCTTT ATGGTTCTGA CATGGACGTA GTTAGCTTTG ATGACGACAG CTCTTCTCCC 
TTTATCCAAA TCCGCTCAGT TGCCAAGAAG CATCCTAAAA CTTGGGTCCA CTATATTGCT 
GCTGAGGAGG AGGACTGGGA CTATGCTCCC TCAGGCCCCA CCCCCAATGA TAGAAGTCAT 
AAAAATCTGT ATTTGAACAA TGGTCCTCAG CGGATTGGTA AGAAGTACAA AAAAGTCCGA 
TTTGTGGCAT ACACAGATGA GACATTTAAG ACTCGTGAAG CTATTCAGTA TGAATCAGGA 
ATCCTGGGAC CTTTACTTTA TGGAGAAGTT GGAGACACAC TGCTGATTAT ATTTAAGAAA 
CAAGCCAGCC GGCCATATAA CATCTACCCT CATGGGATCA ATTATGTCAC TCCTCTGCAC 
ACAGGGAGAT TGCCAAAAGG TGTGAAACAT TTGAAAGATA TGCCAATTCT GCCGGGAGAG 
ATATTCAAGT ATAAATGGAC AGTGACCGTA GAAGATGGAC CAACTAAATC AGATCCTCGG 
TGCCTGACCC GATATTACTC AAGCTTCATT AATCTGGAGA GAGATCTAGC TTCAGGACTC 
ATTGGCCCTC TTCTCATCTG CTACAAAGAA TCTGTAGATC AAAGAGGAAA CCAGATGATG 
TCAGACAAGA GAAATGTCAT CCTGTTTTCT GTACTTGATG AGAATCGAAG CTGGTACCTC 
ACAGAGGATA TGCAGCGCTT CCTCCCCAAT GCAGATGTAG TGCAGCCCCA TGACCCAGAG 
TTCCAACTCT CTAACATCAT GCACAGCATC AATGGCTATG TTTTTGACAA CTTGCAGCTG 
TCAGTTTGTT TGCATGAGGT GGCGTACTGG TACATTCTAA GTGTTGGAGC ACAAACTGAC 
TTCCTGTCTG TCTTCTTCTC TGGATATACC TTCAAACACA AAATGGTCTA TGAAGACACA 
CTTACCCTCT TCCCATTCTC AGGAGAAACT GTCTTCATGT CAATGGAAAA CCCAGGTCTG 
TGGGTTCTGG GGTGCCACAA CTCAGACTTT CGGAACAGAG GCATGACAGC CTTACTGAAG 
GTTTCTAGTT GTAACAGGAA CATTGATGAT TATTATGAGG ACACATACGA AGATATTCCA 
ACTCCCCTGC TAAATGAAAA CAATGTAATT AAACCTAGAA GCTTCTCCCA GAATTCAAGG 
CACCCTAGCA CTAAGGAAAA GCAATTGAAA GCCACCACAA CTCCAGAAAA TGACATAGAG 
AAGATTGACC TTCAATCTGG AGAAAGAACA CAGCTGATTA AAGCACAAAG TGTCTCCTCT 
AGTGATTTGT TGATGCTGTT GGGACAGAAT CCTACTCCAC GTGGACTGTT CTTATCTGAT 
CTCCGAGAGG CCACAGATAG AGCCGATGAC CATTCACGTG GAGCAATAGA AAGAAACAAG 
GGCCCACCTG AAGTGGCAAG TCTCAGACCA GAGCTCCGTC ACAGTGAGGA CAGAGAATTT 
ACTCCTGAGC CAGAACTGCA GTTAAGATTA AATGAGAATT TGGGGACAAA TACAACAGTA 
GAGTTGAAGA AACTTGATTT AAAAATTTCT AGTTCATCAG ACAGTCTAAT GACTTCACCA 
ACAATTCCAT CAGATAAGTT GGCAGCAGCT ACTGAAAAGA CAGGTTCCTT AGGACCCCCA 
AATATGTCAG TTCACTTTAA CGGTCATTTA GGTACCATTG TATTTGGCAA TAATTCATCC 
CACCTTATTC AGTCTGGTGT ACCTTTGGAA TTGAGTGAAG AAGATAACGA TTCCAAGTTG 
TTAGAAGCAC CTTTAATGAA TATTCAAGAA AGTTCACTGA GAGAAAATGT ATTATCAATG 
GAAAGTAATA GGTTATTTAA AGAAGAAAGA ATTCGTGGAC CTGCTTCATT AATCAAAGAT 
AATGCTTTAT TCAAAGTTAA TATCTCTTCG GTAAAGACAA ACAGGGCACC AGTTAACTTA 
ACAACTAATA GAAAGACTCG TGTTGCTATC CCAACATTAT TAATTGAGAA CAGTACCTCA 
GTCTGGCAAG ATATTATGTT AGAAAGGAAT ACTGAGTTTA AAGAAGTAAC TTCTTTGATT 
CATAATGAAA CGTTTATGGA CAGAAATACT ACAGCTCTGG GGCTAAATCA TGTGTCAAAT 
AAAACTACTT TATCAAAAAA TGTGGAAATG GCCCACCAAA AAAAAGAAGA CCCTGTGCCA 
CTACGTGCAG AAAATCCAGA TCTATCATCC TCCAAGATAC CGTTCTTGCC AGATTGGATA 
AAGACCCATG GCAAGAACTC CCTAAGCTCT GAGCAAAGGC CCAGTCCAAA ACAATTAACA 
TCTTTAGGAT CAGAAAAATC TGTGAAAGAT CAGAACTTTT TGTCAGAGGA GAAGGTGGTA 
GTAGGAGAGG ATGAATTTAC GAAGGACACA GAACTCCAAG AGATTTTTCC AAACAACAAG 
AGCATATTTT TTGCTAACTT GGCTAATGTC CAAGAAAATG ATACATACAA TCAAGAAAAA 
AAATCTCTGG AAGAGATAGA AAGAAAGGAA AAATTAACCC AGGAGAATGT GGCTTTGCCT 
CAGGCACATA CTATGATTGG CACTAAGAAC TTCCTGAAGA ACCTTTTCTT ACTAAGCACT 
AAGCAAAATG TAGCAGGTTT AGAAGAGCAG CCATATACTC CAATACTTCA AGACACCAGG 
TCATTAAATG ATTCGCCACA TAGTGAAGGG ATTCATATGG CCAATTTCTC AAAAATAAGG 
GAAGAAGCAA ACTTGGAAGG CTTGGGAAAT CAAACAAACC AAATGGTAGA GAGGTTTCCA 
AGCACTACGA GGATGTCTTC TAATGCAAGT CAGCATGTTA TCACTCAACG TGGTAAGCGG 
AGTTTGAAAC AACCCAGACT CTCACAAGGA GAAATAAAGT TTGAAAGGAA GGTTATTGCA 
AATGACACTT CAACCCAGTG GTCCAAAAAC ATGAACTATT TGGCCCAGGG AACCCTCACA 
CAGATAGAGT ATAATGAGAA AGAAAAAAGG GCCATTACTC AGTCCCCCCT ATCAGATTGT 
TCTATGAGGA ATCATGTCAC CATTCAAATG AATGACTCTG CATTACCCGT TGCAAAGGAA 
TCAGCATCTC CATCAGTTAG ACATACAGAT CTGACCAAGA TCCCATCCCA ACACAACTCT 
TCTCATCTTC CAGCATCGGC CTGTAATTAT ACCTTTAGAG AGAGGACTTC TGGAGTCCAA 
GAAGGCAGTC ATTTCTTACA AGAAGCCAAA AGAAATAACC TCTCTTTAGC CTTTGTAACC 
TTAGGAATAA CTGAAGGGCA AGGAAAGTTC AGCTCCCTGG GGAAAAGTGC CACAAACCAA 
CCCATGTACA AGAAACTTGA AAACACTGTT CTCTTGCAAC CAGGCTTGTC CGAAACATCT 
GACAAAGTTG AATTACTTTC TCAAGTTCAT GTTGATCAAG AAGACTCTTT CCCTACAAAA 
ACTAGCAATG ATTCTCCTGG CCACCTGGAT CTCATGGGAA AGATCTTCCT TCAGAAAACA 
CAGGGACCTG TTAAAATGAA TAAAACAAAT AGCCCTGGAA AAGTGCCCTT TCTGAAATGG 
GCAACAGAAA GCTCTGAAAA GATTCCCTCC AAGCTGCTGG GTGTCCTTGC TTGGGATAAC 
CACTATGATA CCCAGATACC AAGTGAAGAG TGGAAATCCC AAAAAAAGTC ACAGACGAAC 
ACAGCTTTTA AAAGGAAAGA CACCATTTTG CCCCTGGGCC CTTGTGAAAA TAATGATTCA 
ACAGCAGCAA TAAATGAAGG ACAAGATAAG CCCCAAAGAG AAGCTATGTG GGCAAAGCAA 
GGAGAGCCTG GAAGGTTGTG CTCTCAAAAC CCACCAGTCT CAAAACACCA TCAAAGGGAA 
ATAACCGTTA CTACTCTTCA GCCAGAGGAA GACAAATTTG AGTATGATGA CACCTTCTCA 
ATTGAAATGA AGAGAGAAGA TTTTGACATC TACGGCGACT ATGAAGATCA GGGCCTCCGC 
AGCTTTCAAA AGAAAACACG ACACTATTTC ATTGCTGCAG TGGAGCGTCT CTGGGATTAT 
GGGATGAGTA GATCTCCCCA TATACTAAGA AACAGGGCTC AAAGTGGGGA TGTCCAGCAG 
TTCAAGAAGG TGGTTTTCCA GGAATTTACT GATGGATCCT TTACTCAGCC CTTATACCGT 
GGAGAACTGA ATGAACACTT GGGACTCTTG GGGCCATATA TAAGAGCAGA AGTTGAAGAC 
AATATCGTGG TAACTTTCAA AAACCAGGCC TCTCGTCCCT ACTCCTTCTA TTCTAGTCTT 
ATTTCTTATG ACGAAGATGA GGGACAAGGA GCAGAACCTA GAAGAAAGTT TGTCAACCCT 
AATGAAACCA AAATTTACTT TTGGAAAGTA CAGCATCATA TGGCACCCAC TAAAGATGAG 
TTTGACTGCA AAGCCTGGGC TTATTTTTCT GATGTTGATC TGGAGAAAGA TGTGCACTCA 
GGCTTGATTG GACCCCTTCT GATCTGCCGC AGTAACACAC TGAACCCTGC TCATGGGAGA 
CAAGTGACAG TGCAGGAGTT TGCCCTGGTT TTCACTATAT TCGATGAGAC TAAGAGCTGG 
TACTTCACTG AAAACCTGGA AAGGAACTGC AGAGCTCCCT GCAATGTCCA GAAGGAGGAC 
CCTACTCTAA AAGAAAACTT CCGCTTCCAT GCAATCAACG GCTATGTGAA GGATACACTC 
CCTGGCTTAG TAATGGCTCA GGATCAAAAG GTTCGATGGT ATCTGCTCAG CATGGGCAGC 
AACGAAAACA TTCATTCCAT TCACTTCAGT GGACATGTGT TCACTGTACG GAAAAAAGAG 
GAATATAAAA TGGCAGTCTA CAACCTCTAT CCAGGTGTTT TTGAGACTGT GGAAATGCTA 
CCATCCCAAG TTGGAATCTG GCGGATAGAA TGCCTTATCG GCGAGCACCT GCAAGCCGGG 
ATGAGCACTC TGTTTCTGGT GTACAGCAAG AAGTGTCAGA CTCCACTGGG GATGGCTTCC 
GGACACATTA GAGATTTTCA GATTACAGCT TCAGGACAAT ATGGACAGTG GGCCCCAAAG 
CTGGCCAGAC TTCATTATTC CGGATCAATC AATGCCTGGA GCACCAAGGA TCCCTTTTCC 
TGGATCAAGG TGGATCTCTT GGCACCGATG ATTATTCACG GCATCATGAC CCAGGGGGCC 
CGCCAGAAGT TCTCCAGCCT CTACGTGTCT CAGTTTATCA TCATGTACAG TCTGGATGGC 
AACAAGTGGC ACAGTTACCG AGGGAATTCC ACGGGGACCT TAATGGTCTT CTTTGGCAAC 
GTGGATTCAT CTGGGATCAA ACACAATATT TTTAACCCTC CGATTATTGC TCAGTACATC 
CGTTTGCACC CAACCCATTA CAGCATCCGC AGCACTCTTC GCATGGAGCT CTTGGGCTGT 
GACTTCAACA GTTGCAGCAT GCCGCTGGGG ATGGAGAGTA AAGCAATATC AGATGCTCAG 
ATCACTGCCT CGTCCTACCT AAGCAGTATG CTTGCCACTT GGTCTCCTTC CCAAGCCCGG 
CTGCACCTGC AGGGCAGGAC TAATGCCTGG AGACCTCAGG CAAATAACCC AAAAGAGTGG 
CTGCAAGTGG ACTTCCGGAA GACCATGAAA GTCACAGGAA TAACCACCCA GGGGGTGAAA 
TCTCTCCTCA TCAGCATGTA TGTGAAGGAG TTCCTCATCT CCAGTAGTCA AGATGGCCAT 
AACTGGACTC TGTTTCTTCA GAATGACAAA GTCAAGGTCT TCCAGGGAAA CCGGGACTCC 
TCCACGCCTG TGCGGAACGC TCTCGAACCC CCGCTGGTGG CTCGCTACGT GCGCCTGCAC 
CCGCAGAGCT GGGCGCACCA CATCGCCCTG AGGCTGGAGG TCCTGGGCTG CGACACCCAG 
CAGCCCGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

F8 ( XM_005640988.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005641045.1 Canis lupus familiaris coagulation factor VIII, procoagulant component (F8), transcript variant X1, mRNA.

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CloneID	OCe31938
Clone ID Related Accession (Same CDS sequence)	XM_005640988.2 , XM_022415494.1
Accession Version	XM_005640988.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	coagulation factor VIII isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005640988.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCTGC TGGGTCCTAC CATCCAGGCT GAGGTTTATG ACACAGTGGT CATTGTCCTT 
AAGAACATGG CTTCTCATCC TGTCAGCCTT CACGCTGTTG GTGTATCCTA TTGGAAAGCT 
TCTGAAGGTG CTGAGTATGA GGATCAGACC AGCCAAAAGG AGAAGGAAGA TGATAATGTC 
ATTCCTGGTG AAAGCCATAC CTATGTCTGG CAGGTCCTGA AAGAGAATGG CCCAATGGCC 
TCTGATCCAC CATGTCTCAC CTACTCATAT TTTTCACACG TGGACCTGGT GAAAGACCTG 
AATTCAGGCC TCATTGGAGC CCTGCTGGTT TGCAAAGAAG GGAGTCTGGC CAAAGAAAGG 
ACACAGACCT TGCAGGAATT TGTCCTACTT TTTGCTGTAT TTGATGAAGG GAAAAGTTGG 
CACTCAGAAA CAAATGCGTC TTTGACACAG GCTGAGGCCC AGCATGAGCT GCACACCATC 
AATGGCTATG TAAACAGGTC TCTGCCAGGT CTTACTGTGT GTCACAAGAG ATCAGTCTAT 
TGGCATGTGA TTGGAATGGG CACCACCCCC GAAGTGCACT CAATTTTTCT CGAAGGTCAC 
ACATTTCTTG TGAGGAACCA CCGCCAGGCC TCCTTGGAGA TCTCACCAAT TACTTTCCTT 
ACTGCTCAGA CATTCCTGAT GGACCTTGGC CAGTTTCTAC TGTTTTGTCA TATCCCTTCC 
CATCAACATG ATGGTATGGA AGCTTATGTC AAAGTAGATA GCTGCCCAGA GGAACCCCAG 
CTGCGCATGA AAAATAATGA AGATAAAGAT TATGATGATG GTCTTTATGA TTCTGACATG 
GACGTAGTTA GCTTTGATGA CGACAGCTCT TCTCCCTTTA TCCAAATCCG CTCAGTTGCC 
AAGAAGCATC CTAAAACTTG GGTCCACTAT ATTGCTGCTG AGGAGGAGGA CTGGGACTAT 
GCTCCCTCAG GCCCCACCCC CAATGATAGA AGTCATAAAA ATCTGTATTT GAACAATGGT 
CCTCAGCGGA TTGGTAAGAA GTACAAAAAA GTCCGATTTG TGGCATACAC AGATGAGACA 
TTTAAGACTC GTGAAGCTAT TCAGTATGAA TCAGGAATCC TGGGACCTTT ACTTTATGGA 
GAAGTTGGAG ACACACTGCT GATTATATTT AAGAATCAAG CCAGCCGGCC ATATAACATC 
TACCCTCATG GGATCAATTA TGTCACTCCT CTGCACACAG GGAGATTGCC AAAAGGTGTG 
AAACATTTGA AAGATATGCC AATTCTGCCG GGAGAGATAT TCAAGTATAA ATGGACAGTG 
ACCGTAGAAG ATGGACCAAC TAAATCAGAT CCTCGGTGCC TGACCCGATA TTACTCAAGC 
TTCATTAATC TGGAGAGAGA TCTAGCTTCA GGACTCATTG GCCCTCTTCT CATCTGCTAC 
AAAGAATCTG TAGATCAAAG AGGAAACCAG ATGATGTCAG ACAAGAGAAA TGTCATCCTG 
TTTTCTGTAT TTGATGAGAA TCGAAGCTGG TACCTCACAG AGAATATGCA GCGCTTCCTC 
CCCAATGCAG ATGTAGTGCA GCCCCATGAC CCAGAGTTCC AACTCTCTAA CATCATGCAC 
AGCATCAATG GCTATGTTTT TGACAACTTG CAGCTGTCAG TTTGTTTGCA TGAGGTGGCG 
TACTGGTACA TTCTAAGTGT TGGAGCACAA ACTGACTTCC TGTCTGTCTT CTTCTCTGGA 
TATACCTTCA AACACAAAAT GGTCTATGAA GACACACTTA CCCTCTTCCC ATTCTCAGGA 
GAAACTGTCT TCATGTCAAT GGAAAACCCA GGTCTGTGGG TTCTGGGGTG CCACAACTCA 
GACTTTCGGA ACAGAGGCAT GACAGCCTTA CTGAAGGTTT CTAGTTGTAA CAGGAACATT 
GATGATTATT ATGAGGACAC ATACGAAGAT ATTCCAACTC CCCTGCTAAA TGAAAACAAT 
GTAATTAAAC CTAGAAGCTT CTCCCAGAAT TCAAGGCACC CTAGCACTAA GGAAAAGCAA 
TTGAAAGCCA CCACAACTCC AGAAAATGAC ATAGAGAAGA TTGACCTTCA ATCTGGAGAA 
AGAACACAGC TGATTAAAGC ACAAAGTGTC TCCTCTAGTG ATTTGTTGAT GCTGTTGGGA 
CAGAATCCTA CTCCACGTGG ACTGTTCTTA TCTGATCTCC GAGAGGCCAC AGATAGAGCC 
GATGACCATT CACGTGGAGC AATAGAAAGA AACAAGGGCC CACCTGAAGT GGCAAGTCTC 
AGACCAGAGC TCCGTCACAG TGAGGACAGA GAATTTACTC CTGAGCCAGA ACTGCAGTTA 
AGATTAAATG AGAATTTGGG GACAAATACA ACAGTAGAGT TGAAGAAACT TGATTTAAAA 
ATTTCTAGTT CATCAGACAG TCTAATGACT TCACCAACAA TTCCATCAGA TAAGTTGGCA 
GCAGCTACTG AAAAGACAGG TTCCTTAGGA CCCCCAAATA TGTCAGTTCA CTTTAACAGT 
CATTTAGGTA CCATTGTATT TGGCAATAAT TCATCCCACC TTATTCAGTC TGGTGTACCT 
TTGGAATTGA GTGAAGAAGA TAATGATTCC AAGTTGTTAG AAGCACCTTT AATGAATATT 
CAAGAAAGTT CACTGAGAGA AAATGTATTA TCAATGGAGA GTAATAGGTT ATTTAAAGAA 
GAAAGAATTC GTGGACCTGC TTCATTAATC AAAGATAATG CTTTATTCAA AGTTAATATC 
TCTTCGGTAA AGACAAACAG GGCACCAGTT AACTTAACAA CTAATAGAAA GACTCGTGTT 
GCTATCCCAA CATTATTAAT TGAGAACAGT ACCTCAGTCT GGCAAGATAT TATGTTAGAA 
AGGAATACTG AGTTTAAAGA AGTAACTTCT TTGATTCATA ATGAAACGTT TATGGACAGA 
AATACTACAG CTCTGGGGCT AAATCATGTG TCAAATAAAA CTACTTTATC AAAAAATGTG 
GAAATGGCCC ACCAAAAAAA AGAAGACCCT GTGCCACTAC GTGCAGAAAA TCCAGATCTA 
TCATCCTCCA AGATACCGTT CTTGCCAGAT TGGATAAAGA CCCATGGCAA GAACTCCCTA 
AGCTCTGAGC AAAGGCCCAG TCCAAAACAA TTAACATCTT TAGGATCAGA AAAATCTGTG 
AAAGATCAGA ACTTTTTGTC AGAGGAGAAG GTGGTAGTAG GAGAGGATGA ATTTACGAAG 
GACACAGAAC TCCAAGAGAT TTTTCCAAAC AACAAGAGCA TATTTTTTGC TAACTTGGCT 
AATGTCCAAG AAAATGATAC ATACAATCAA GAAAAAAAAT CTCCGGAAGA GATAGAAAGA 
AAGGAAAAAT TAACCCAGGA GAATGTGGCT TTGCCTCAGG CACATACTAT GATTGGCACT 
AAGAACTTCC TGAAGAACCT TTTCTTACTA AGCACTAAGC AAAATGTAGC AGGTTTAGAA 
GAGCAGCCAT ATACTCCAAT ACTTCAAGAC ACCAGGTCAT TAAATGATTC GCCACATAGT 
GAAGGGATTC ATATGGCCAA TTTCTCAAAA ATAAGGGAAG AAGCAAACTT GGAAGGCTTG 
GGAAATCAAA CAAACCAAAT GGTAGAGAGG TTTCCAAGCA CTACGAGGAT GTCTTCTAAT 
GCAAGTCAGC ATGTTATCAC TCAACGTGGT AAGCGGAGTT TGAAACAACC CAGACTCTCA 
CAAGGAGAAA TAAAGTTTGA AAGGAAGGTT ATTGCAAATG ACACTTCAAC CCAGTGGTCC 
AAAAACATGA ACTATTTGGC CCAGGGAACC CTCACACAGA TAGAGTATAA TGAGAAAGAA 
AAAAGGGCCA TTACTCAGTC CCCCCTATCA GATTGTTCTA TGAGGAATCA TGTCACCATT 
CAAATGAATG ACTCTGCATT ACCCGTTGCA AAGGAATCAG CATCTCCATC AGTTAGACAT 
ACAGATCTGA CCAAGATCCC ATCCCAACAC AACTCTTCTC ATCTTCCAGC ATCAGCCTGT 
AATTATACCT TTAGAGAGAG GACTTCTGGA GTCCAAGAAG GCAGTCATTT CTTACAAGAA 
GCCAAAAGAA ATAACCTCTC TTTAGCCTTT GTAACCTTAG GAATAACTGA AGGGCAAGGA 
AAGTTCAGCT CCCTGGGGAA AAGTGCCACA AACCAACCCA TGTACAAGAA ACTTGAAAAC 
ACTGTTCTCT TGCAACCAGG CTTGTCCGAA ACATCTGACA AAGTTGAATT ACTTTCTCAA 
GTTCATGTTG ATCAAGAAGA CTCTTTCCCT ACAAAAACTA GCAATGATTC TCCTGGCCAC 
CTGGATCTCA TGGGAAAGAT CTTCCTTCAG AAAACACAGG GACCTGTTAA AATGAATAAA 
ACAAATAGCC CTGGAAAAGT GCCCTTTCTG AAATGGGCAA CAGAAAGCTC TGAAAAGATT 
CCCTCCAAGC TGCTGGGTGT CCTTGCTTGG GATAACCACT ATGATACCCA GATACCAAGT 
GAAGAGTGGA AATCCCAAAA AAAGTCACAG ACGAACACAG CTTTTAAAAG GAAAGACACC 
ATTTTGCCCC TGGGCCCTTG TGAAAATAAT GATTCAACAG CAGCAATAAA TGAAGGACAA 
GATAAGCCCC AAAGAGAAGC TATGTGGGCA AAGCAAGGAG AGCCTGGAAG GTTGTGCTCT 
CAAAACCCAC CAGTCTCAAA ACACCATCAA AGGGAAATAA CCGTTACTAC TCTTCAGCCA 
GAGGAAGACA AATTTGAGTA TGATGACACC TTCTCAATTG AAATGAAGAG AGAAGATTTT 
GACATCTACG GCGACTATGA AAATCAGGGC CTCCGCAGCT TTCAAAAGAA AACACGACAC 
TATTTCATTG CTGCAGTGGA GCGTCTCTGG GATTATGGGA TGAGTAGATC TCCCCATATA 
CTAAGAAACA GGGCTCAAAG TGGGGATGTC CAGCAGTTCA AGAAGGTGGT TTTCCAGGAA 
TTTACTGATG GATCCTTTAC TCAGCCCTTA TACCGTGGAG AACTGAACGA ACACTTGGGA 
CTCTTGGGGC CATATATAAG AGCAGAAGTT GAAGACAATA TCGTGGTAAC TTTCAAAAAC 
CAGGCCTCTC GTCCCTACTC CTTCTATTCT AGTCTTATTT CTTATGACGA AGATGAGGGA 
CAAGGAGCAG AACCTAGAAG AAAGTTTGTC AACCCTAATG AAACCAAAAT TTACTTTTGG 
AAAGTGCAGC ATCATATGGC ACCCACTAAA GATGAGTTTG ACTGCAAAGC CTGGGCTTAT 
TTTTCTGATG TTGATCTGGA GAAAGATGTG CACTCAGGCT TGATTGGACC CCTTCTGATC 
TGCCGCAGTA ACACACTGAA CCCTGCTCAT GGGAGACAAG TGACAGTGCA GGAGTTTGCC 
CTGGTTTTCA CTATATTCGA TGAGACTAAG AGCTGGTACT TCACTGAAAA CCTGGAAAGG 
AACTGCAGAG CTCCCTGCAA TGTCCAGAAG GAGGACCCTA CTCTAAAAGA AAACTTCCGC 
TTCCATGCAA TCAACGGCTA TGTGAAGGAT ACACTCCCTG GCTTAGTAAT GGCTCAGGAT 
CAAAAGGTTC GATGGTATCT GCTCAGCATG GGCAGCAACG AAAACATTCA TTCCATTCAC 
TTCAGTGGAC ATGTGTTCAC TGTACGGAAA AAAGAGGAAT ATAAAATGGC AGTCTACAAC 
CTCTATCCAG GTGTTTTTGA GACTGTGGAA ATGCTACCAT CCCAAGTTGG AATCTGGCGG 
ATAGAATGCC TTATCGGCGA GCACCTGCAA GCCGGGATGA GCACTCTGTT TCTGGTGTAC 
AGCAAGAAGT GTCAGACTCC ACTGGGGATG GCTTCCGGAC ACATTAGAGA TTTTCAGATT 
ACAGCTTCAG GACAATATGG ACAGTGGGCC CCAAAGCTGG CCAGACTTCA TTATTCCGGA 
TCAATCAATG CCTGGAGCAC CAAGGATCCC TTTTCCTGGA TCAAGGTGGA TCTCTTGGCA 
CCGATGATTA TTCACGGCAT CATGACCCAG GGGGCCCGCC AGAAGTTCTC CAGCCTCTAC 
GTGTCTCAGT TTATCATCAT GTACAGTCTG GATGGCAACA AGTGGCACAG TTACCGAGGG 
AATTCCACGG GGACCTTAAT GGTCTTCTTT GGCAACGTGG ATTCATCTGG GATCAAACAC 
AATATTTTTA ACCCTCCGAT TATTGCTCAG TACATCCGTT TGCACCCAAC CCATTACAGC 
ATCCGCAGCA CTCTTCGCAT GGAGCTCTTG GGCTGTGACT TCAACAGTTG CAGCATGCCG 
CTGGGGATGG AGAGTAAAGC AATATCAGAT GCTCAGATCA CTGCCTCGTC CTACCTAAGC 
AGTATGCTTG CCACTTGGTC TCCTTCCCAA GCCCGGCTGC ACCTGCAGGG CAGGACTAAT 
GCCTGGAGAC CTCAGGCAAA TAACCCAAAA GAGTGGCTGC AAGTGGACTT CCGGAAGACC 
ATGAAAGTCA CAGGAATAAC CACCCAGGGG GTGAAATCTC TCCTCATCAG CATGTATGTG 
AAGGAGTTCC TCATCTCCAG TAGTCAAGAT GGCCATAACT GGACTCTGTT TCTTCAGAAT 
GGCAAAGTCA AGGTCTTCCA GGGAAACCGG GACTCCTCCA CGCCTGTGCG GAACGCTCTC 
GAACCCCCGC TGGTGGCTCG CTACGTGCGC CTGCACCCGC AGAGCTGGGC GCACCACATC 
GCCCTGAGGC TGGAGGTCCT GGGCTGCGAC ACCCAGCAGC CCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005641045.1
CDS	144..6911
Translation	MGLLGPTIQAEVYDTVVIVLKNMASHPVSLHAVGVSYWKASEGAEYEDQTSQKEKEDDNVIPGESHTYVWQVLKENGPMASDPPCLTYSYFSHVDLVKDLNSGLIGALLVCKEGSLAKERTQTLQEFVLLFAVFDEGKSWHSETNASLTQAEAQHELHTINGYVNRSLPGLTVCHKRSVYWHVIGMGTTPEVHSIFLEGHTFLVRNHRQASLEISPITFLTAQTFLMDLGQFLLFCHIPSHQHDGMEAYVKVDSCPEEPQLRMKNNEDKDYDDGLYDSDMDVVSFDDDSSSPFIQIRSVAKKHPKTWVHYIAAEEEDWDYAPSGPTPNDRSHKNLYLNNGPQRIGKKYKKVRFVAYTDETFKTREAIQYESGILGPLLYGEVGDTLLIIFKNQASRPYNIYPHGINYVTPLHTGRLPKGVKHLKDMPILPGEIFKYKWTVTVEDGPTKSDPRCLTRYYSSFINLERDLASGLIGPLLICYKESVDQRGNQMMSDKRNVILFSVFDENRSWYLTENMQRFLPNADVVQPHDPEFQLSNIMHSINGYVFDNLQLSVCLHEVAYWYILSVGAQTDFLSVFFSGYTFKHKMVYEDTLTLFPFSGETVFMSMENPGLWVLGCHNSDFRNRGMTALLKVSSCNRNIDDYYEDTYEDIPTPLLNENNVIKPRSFSQNSRHPSTKEKQLKATTTPENDIEKIDLQSGERTQLIKAQSVSSSDLLMLLGQNPTPRGLFLSDLREATDRADDHSRGAIERNKGPPEVASLRPELRHSEDREFTPEPELQLRLNENLGTNTTVELKKLDLKISSSSDSLMTSPTIPSDKLAAATEKTGSLGPPNMSVHFNSHLGTIVFGNNSSHLIQSGVPLELSEEDNDSKLLEAPLMNIQESSLRENVLSMESNRLFKEERIRGPASLIKDNALFKVNISSVKTNRAPVNLTTNRKTRVAIPTLLIENSTSVWQDIMLERNTEFKEVTSLIHNETFMDRNTTALGLNHVSNKTTLSKNVEMAHQKKEDPVPLRAENPDLSSSKIPFLPDWIKTHGKNSLSSEQRPSPKQLTSLGSEKSVKDQNFLSEEKVVVGEDEFTKDTELQEIFPNNKSIFFANLANVQENDTYNQEKKSPEEIERKEKLTQENVALPQAHTMIGTKNFLKNLFLLSTKQNVAGLEEQPYTPILQDTRSLNDSPHSEGIHMANFSKIREEANLEGLGNQTNQMVERFPSTTRMSSNASQHVITQRGKRSLKQPRLSQGEIKFERKVIANDTSTQWSKNMNYLAQGTLTQIEYNEKEKRAITQSPLSDCSMRNHVTIQMNDSALPVAKESASPSVRHTDLTKIPSQHNSSHLPASACNYTFRERTSGVQEGSHFLQEAKRNNLSLAFVTLGITEGQGKFSSLGKSATNQPMYKKLENTVLLQPGLSETSDKVELLSQVHVDQEDSFPTKTSNDSPGHLDLMGKIFLQKTQGPVKMNKTNSPGKVPFLKWATESSEKIPSKLLGVLAWDNHYDTQIPSEEWKSQKKSQTNTAFKRKDTILPLGPCENNDSTAAINEGQDKPQREAMWAKQGEPGRLCSQNPPVSKHHQREITVTTLQPEEDKFEYDDTFSIEMKREDFDIYGDYENQGLRSFQKKTRHYFIAAVERLWDYGMSRSPHILRNRAQSGDVQQFKKVVFQEFTDGSFTQPLYRGELNEHLGLLGPYIRAEVEDNIVVTFKNQASRPYSFYSSLISYDEDEGQGAEPRRKFVNPNETKIYFWKVQHHMAPTKDEFDCKAWAYFSDVDLEKDVHSGLIGPLLICRSNTLNPAHGRQVTVQEFALVFTIFDETKSWYFTENLERNCRAPCNVQKEDPTLKENFRFHAINGYVKDTLPGLVMAQDQKVRWYLLSMGSNENIHSIHFSGHVFTVRKKEEYKMAVYNLYPGVFETVEMLPSQVGIWRIECLIGEHLQAGMSTLFLVYSKKCQTPLGMASGHIRDFQITASGQYGQWAPKLARLHYSGSINAWSTKDPFSWIKVDLLAPMIIHGIMTQGARQKFSSLYVSQFIIMYSLDGNKWHSYRGNSTGTLMVFFGNVDSSGIKHNIFNPPIIAQYIRLHPTHYSIRSTLRMELLGCDFNSCSMPLGMESKAISDAQITASSYLSSMLATWSPSQARLHLQGRTNAWRPQANNPKEWLQVDFRKTMKVTGITTQGVKSLLISMYVKEFLISSSQDGHNWTLFLQNGKVKVFQGNRDSSTPVRNALEPPLVARYVRLHPQSWAHHIALRLEVLGCDTQQPA

Target ORF information:

RefSeq Version	XM_005640988.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris coagulation factor VIII, procoagulant component (F8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005640988.2

ORF Insert Sequence:

ATGGGCCTGC TGGGTCCTAC CATCCAGGCT GAGGTTTATG ACACAGTGGT CATTGTCCTT 
AAGAACATGG CTTCTCATCC TGTCAGCCTT CACGCTGTTG GTGTATCCTA TTGGAAAGCT 
TCTGAAGGTG CTGAGTATGA GGATCAGACC AGCCAAAAGG AGAAGGAAGA TGATAATGTC 
ATTCCTGGTG AAAGCCATAC CTATGTCTGG CAGGTCCTGA AAGAGAATGG CCCAATGGCC 
TCTGATCCAC CATGTCTCAC CTACTCATAT TTTTCACACG TGGACCTGGT GAAAGACCTG 
AATTCAGGCC TCATTGGAGC CCTGCTGGTT TGCAAAGAAG GGAGTCTGGC CAAAGAAAGG 
ACACAGACCT TGCAGGAATT TGTCCTACTT TTTGCTGTAT TTGATGAAGG GAAAAGTTGG 
CACTCAGAAA CAAATGCGTC TTTGACACAG GCTGAGGCCC AGCATGAGCT GCACACCATC 
AATGGCTATG TAAACAGGTC TCTGCCAGGT CTTACTGTGT GTCACAAGAG ATCAGTCTAT 
TGGCATGTGA TTGGAATGGG CACCACCCCC GAAGTGCACT CAATTTTTCT CGAAGGTCAC 
ACATTTCTTG TGAGGAACCA CCGCCAGGCC TCCTTGGAGA TCTCACCAAT TACTTTCCTT 
ACTGCTCAGA CATTCCTGAT GGACCTTGGC CAGTTTCTAC TGTTTTGTCA TATCCCTTCC 
CATCAACATG ATGGTATGGA AGCTTATGTC AAAGTAGATA GCTGCCCAGA GGAACCCCAG 
CTGCGCATGA AAAATAATGA AGATAAAGAT TATGATGATG GTCTTTATGA TTCTGACATG 
GACGTAGTTA GCTTTGATGA CGACAGCTCT TCTCCCTTTA TCCAAATCCG CTCAGTTGCC 
AAGAAGCATC CTAAAACTTG GGTCCACTAT ATTGCTGCTG AGGAGGAGGA CTGGGACTAT 
GCTCCCTCAG GCCCCACCCC CAATGATAGA AGTCATAAAA ATCTGTATTT GAACAATGGT 
CCTCAGCGGA TTGGTAAGAA GTACAAAAAA GTCCGATTTG TGGCATACAC AGATGAGACA 
TTTAAGACTC GTGAAGCTAT TCAGTATGAA TCAGGAATCC TGGGACCTTT ACTTTATGGA 
GAAGTTGGAG ACACACTGCT GATTATATTT AAGAATCAAG CCAGCCGGCC ATATAACATC 
TACCCTCATG GGATCAATTA TGTCACTCCT CTGCACACAG GGAGATTGCC AAAAGGTGTG 
AAACATTTGA AAGATATGCC AATTCTGCCG GGAGAGATAT TCAAGTATAA ATGGACAGTG 
ACCGTAGAAG ATGGACCAAC TAAATCAGAT CCTCGGTGCC TGACCCGATA TTACTCAAGC 
TTCATTAATC TGGAGAGAGA TCTAGCTTCA GGACTCATTG GCCCTCTTCT CATCTGCTAC 
AAAGAATCTG TAGATCAAAG AGGAAACCAG ATGATGTCAG ACAAGAGAAA TGTCATCCTG 
TTTTCTGTAT TTGATGAGAA TCGAAGCTGG TACCTCACAG AGAATATGCA GCGCTTCCTC 
CCCAATGCAG ATGTAGTGCA GCCCCATGAC CCAGAGTTCC AACTCTCTAA CATCATGCAC 
AGCATCAATG GCTATGTTTT TGACAACTTG CAGCTGTCAG TTTGTTTGCA TGAGGTGGCG 
TACTGGTACA TTCTAAGTGT TGGAGCACAA ACTGACTTCC TGTCTGTCTT CTTCTCTGGA 
TATACCTTCA AACACAAAAT GGTCTATGAA GACACACTTA CCCTCTTCCC ATTCTCAGGA 
GAAACTGTCT TCATGTCAAT GGAAAACCCA GGTCTGTGGG TTCTGGGGTG CCACAACTCA 
GACTTTCGGA ACAGAGGCAT GACAGCCTTA CTGAAGGTTT CTAGTTGTAA CAGGAACATT 
GATGATTATT ATGAGGACAC ATACGAAGAT ATTCCAACTC CCCTGCTAAA TGAAAACAAT 
GTAATTAAAC CTAGAAGCTT CTCCCAGAAT TCAAGGCACC CTAGCACTAA GGAAAAGCAA 
TTGAAAGCCA CCACAACTCC AGAAAATGAC ATAGAGAAGA TTGACCTTCA ATCTGGAGAA 
AGAACACAGC TGATTAAAGC ACAAAGTGTC TCCTCTAGTG ATTTGTTGAT GCTGTTGGGA 
CAGAATCCTA CTCCACGTGG ACTGTTCTTA TCTGATCTCC GAGAGGCCAC AGATAGAGCC 
GATGACCATT CACGTGGAGC AATAGAAAGA AACAAGGGCC CACCTGAAGT GGCAAGTCTC 
AGACCAGAGC TCCGTCACAG TGAGGACAGA GAATTTACTC CTGAGCCAGA ACTGCAGTTA 
AGATTAAATG AGAATTTGGG GACAAATACA ACAGTAGAGT TGAAGAAACT TGATTTAAAA 
ATTTCTAGTT CATCAGACAG TCTAATGACT TCACCAACAA TTCCATCAGA TAAGTTGGCA 
GCAGCTACTG AAAAGACAGG TTCCTTAGGA CCCCCAAATA TGTCAGTTCA CTTTAACAGT 
CATTTAGGTA CCATTGTATT TGGCAATAAT TCATCCCACC TTATTCAGTC TGGTGTACCT 
TTGGAATTGA GTGAAGAAGA TAATGATTCC AAGTTGTTAG AAGCACCTTT AATGAATATT 
CAAGAAAGTT CACTGAGAGA AAATGTATTA TCAATGGAGA GTAATAGGTT ATTTAAAGAA 
GAAAGAATTC GTGGACCTGC TTCATTAATC AAAGATAATG CTTTATTCAA AGTTAATATC 
TCTTCGGTAA AGACAAACAG GGCACCAGTT AACTTAACAA CTAATAGAAA GACTCGTGTT 
GCTATCCCAA CATTATTAAT TGAGAACAGT ACCTCAGTCT GGCAAGATAT TATGTTAGAA 
AGGAATACTG AGTTTAAAGA AGTAACTTCT TTGATTCATA ATGAAACGTT TATGGACAGA 
AATACTACAG CTCTGGGGCT AAATCATGTG TCAAATAAAA CTACTTTATC AAAAAATGTG 
GAAATGGCCC ACCAAAAAAA AGAAGACCCT GTGCCACTAC GTGCAGAAAA TCCAGATCTA 
TCATCCTCCA AGATACCGTT CTTGCCAGAT TGGATAAAGA CCCATGGCAA GAACTCCCTA 
AGCTCTGAGC AAAGGCCCAG TCCAAAACAA TTAACATCTT TAGGATCAGA AAAATCTGTG 
AAAGATCAGA ACTTTTTGTC AGAGGAGAAG GTGGTAGTAG GAGAGGATGA ATTTACGAAG 
GACACAGAAC TCCAAGAGAT TTTTCCAAAC AACAAGAGCA TATTTTTTGC TAACTTGGCT 
AATGTCCAAG AAAATGATAC ATACAATCAA GAAAAAAAAT CTCCGGAAGA GATAGAAAGA 
AAGGAAAAAT TAACCCAGGA GAATGTGGCT TTGCCTCAGG CACATACTAT GATTGGCACT 
AAGAACTTCC TGAAGAACCT TTTCTTACTA AGCACTAAGC AAAATGTAGC AGGTTTAGAA 
GAGCAGCCAT ATACTCCAAT ACTTCAAGAC ACCAGGTCAT TAAATGATTC GCCACATAGT 
GAAGGGATTC ATATGGCCAA TTTCTCAAAA ATAAGGGAAG AAGCAAACTT GGAAGGCTTG 
GGAAATCAAA CAAACCAAAT GGTAGAGAGG TTTCCAAGCA CTACGAGGAT GTCTTCTAAT 
GCAAGTCAGC ATGTTATCAC TCAACGTGGT AAGCGGAGTT TGAAACAACC CAGACTCTCA 
CAAGGAGAAA TAAAGTTTGA AAGGAAGGTT ATTGCAAATG ACACTTCAAC CCAGTGGTCC 
AAAAACATGA ACTATTTGGC CCAGGGAACC CTCACACAGA TAGAGTATAA TGAGAAAGAA 
AAAAGGGCCA TTACTCAGTC CCCCCTATCA GATTGTTCTA TGAGGAATCA TGTCACCATT 
CAAATGAATG ACTCTGCATT ACCCGTTGCA AAGGAATCAG CATCTCCATC AGTTAGACAT 
ACAGATCTGA CCAAGATCCC ATCCCAACAC AACTCTTCTC ATCTTCCAGC ATCAGCCTGT 
AATTATACCT TTAGAGAGAG GACTTCTGGA GTCCAAGAAG GCAGTCATTT CTTACAAGAA 
GCCAAAAGAA ATAACCTCTC TTTAGCCTTT GTAACCTTAG GAATAACTGA AGGGCAAGGA 
AAGTTCAGCT CCCTGGGGAA AAGTGCCACA AACCAACCCA TGTACAAGAA ACTTGAAAAC 
ACTGTTCTCT TGCAACCAGG CTTGTCCGAA ACATCTGACA AAGTTGAATT ACTTTCTCAA 
GTTCATGTTG ATCAAGAAGA CTCTTTCCCT ACAAAAACTA GCAATGATTC TCCTGGCCAC 
CTGGATCTCA TGGGAAAGAT CTTCCTTCAG AAAACACAGG GACCTGTTAA AATGAATAAA 
ACAAATAGCC CTGGAAAAGT GCCCTTTCTG AAATGGGCAA CAGAAAGCTC TGAAAAGATT 
CCCTCCAAGC TGCTGGGTGT CCTTGCTTGG GATAACCACT ATGATACCCA GATACCAAGT 
GAAGAGTGGA AATCCCAAAA AAAGTCACAG ACGAACACAG CTTTTAAAAG GAAAGACACC 
ATTTTGCCCC TGGGCCCTTG TGAAAATAAT GATTCAACAG CAGCAATAAA TGAAGGACAA 
GATAAGCCCC AAAGAGAAGC TATGTGGGCA AAGCAAGGAG AGCCTGGAAG GTTGTGCTCT 
CAAAACCCAC CAGTCTCAAA ACACCATCAA AGGGAAATAA CCGTTACTAC TCTTCAGCCA 
GAGGAAGACA AATTTGAGTA TGATGACACC TTCTCAATTG AAATGAAGAG AGAAGATTTT 
GACATCTACG GCGACTATGA AAATCAGGGC CTCCGCAGCT TTCAAAAGAA AACACGACAC 
TATTTCATTG CTGCAGTGGA GCGTCTCTGG GATTATGGGA TGAGTAGATC TCCCCATATA 
CTAAGAAACA GGGCTCAAAG TGGGGATGTC CAGCAGTTCA AGAAGGTGGT TTTCCAGGAA 
TTTACTGATG GATCCTTTAC TCAGCCCTTA TACCGTGGAG AACTGAACGA ACACTTGGGA 
CTCTTGGGGC CATATATAAG AGCAGAAGTT GAAGACAATA TCGTGGTAAC TTTCAAAAAC 
CAGGCCTCTC GTCCCTACTC CTTCTATTCT AGTCTTATTT CTTATGACGA AGATGAGGGA 
CAAGGAGCAG AACCTAGAAG AAAGTTTGTC AACCCTAATG AAACCAAAAT TTACTTTTGG 
AAAGTGCAGC ATCATATGGC ACCCACTAAA GATGAGTTTG ACTGCAAAGC CTGGGCTTAT 
TTTTCTGATG TTGATCTGGA GAAAGATGTG CACTCAGGCT TGATTGGACC CCTTCTGATC 
TGCCGCAGTA ACACACTGAA CCCTGCTCAT GGGAGACAAG TGACAGTGCA GGAGTTTGCC 
CTGGTTTTCA CTATATTCGA TGAGACTAAG AGCTGGTACT TCACTGAAAA CCTGGAAAGG 
AACTGCAGAG CTCCCTGCAA TGTCCAGAAG GAGGACCCTA CTCTAAAAGA AAACTTCCGC 
TTCCATGCAA TCAACGGCTA TGTGAAGGAT ACACTCCCTG GCTTAGTAAT GGCTCAGGAT 
CAAAAGGTTC GATGGTATCT GCTCAGCATG GGCAGCAACG AAAACATTCA TTCCATTCAC 
TTCAGTGGAC ATGTGTTCAC TGTACGGAAA AAAGAGGAAT ATAAAATGGC AGTCTACAAC 
CTCTATCCAG GTGTTTTTGA GACTGTGGAA ATGCTACCAT CCCAAGTTGG AATCTGGCGG 
ATAGAATGCC TTATCGGCGA GCACCTGCAA GCCGGGATGA GCACTCTGTT TCTGGTGTAC 
AGCAAGAAGT GTCAGACTCC ACTGGGGATG GCTTCCGGAC ACATTAGAGA TTTTCAGATT 
ACAGCTTCAG GACAATATGG ACAGTGGGCC CCAAAGCTGG CCAGACTTCA TTATTCCGGA 
TCAATCAATG CCTGGAGCAC CAAGGATCCC TTTTCCTGGA TCAAGGTGGA TCTCTTGGCA 
CCGATGATTA TTCACGGCAT CATGACCCAG GGGGCCCGCC AGAAGTTCTC CAGCCTCTAC 
GTGTCTCAGT TTATCATCAT GTACAGTCTG GATGGCAACA AGTGGCACAG TTACCGAGGG 
AATTCCACGG GGACCTTAAT GGTCTTCTTT GGCAACGTGG ATTCATCTGG GATCAAACAC 
AATATTTTTA ACCCTCCGAT TATTGCTCAG TACATCCGTT TGCACCCAAC CCATTACAGC 
ATCCGCAGCA CTCTTCGCAT GGAGCTCTTG GGCTGTGACT TCAACAGTTG CAGCATGCCG 
CTGGGGATGG AGAGTAAAGC AATATCAGAT GCTCAGATCA CTGCCTCGTC CTACCTAAGC 
AGTATGCTTG CCACTTGGTC TCCTTCCCAA GCCCGGCTGC ACCTGCAGGG CAGGACTAAT 
GCCTGGAGAC CTCAGGCAAA TAACCCAAAA GAGTGGCTGC AAGTGGACTT CCGGAAGACC 
ATGAAAGTCA CAGGAATAAC CACCCAGGGG GTGAAATCTC TCCTCATCAG CATGTATGTG 
AAGGAGTTCC TCATCTCCAG TAGTCAAGAT GGCCATAACT GGACTCTGTT TCTTCAGAAT 
GGCAAAGTCA AGGTCTTCCA GGGAAACCGG GACTCCTCCA CGCCTGTGCG GAACGCTCTC 
GAACCCCCGC TGGTGGCTCG CTACGTGCGC CTGCACCCGC AGAGCTGGGC GCACCACATC 
GCCCTGAGGC TGGAGGTCCT GGGCTGCGAC ACCCAGCAGC CCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

F8 ( XM_022415494.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022271202.1 Canis lupus familiaris coagulation factor VIII (F8), transcript variant X1, mRNA.

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CloneID	OCe31938
Clone ID Related Accession (Same CDS sequence)	XM_005640988.2 , XM_022415494.1
Accession Version	XM_022415494.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	coagulation factor VIII isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCTGC TGGGTCCTAC CATCCAGGCT GAGGTTTATG ACACAGTGGT CATTGTCCTT 
AAGAACATGG CTTCTCATCC TGTCAGCCTT CACGCTGTTG GTGTATCCTA TTGGAAAGCT 
TCTGAAGGTG CTGAGTATGA GGATCAGACC AGCCAAAAGG AGAAGGAAGA TGATAATGTC 
ATTCCTGGTG AAAGCCATAC CTATGTCTGG CAGGTCCTGA AAGAGAATGG CCCAATGGCC 
TCTGATCCAC CATGTCTCAC CTACTCATAT TTTTCACACG TGGACCTGGT GAAAGACCTG 
AATTCAGGCC TCATTGGAGC CCTGCTGGTT TGCAAAGAAG GGAGTCTGGC CAAAGAAAGG 
ACACAGACCT TGCAGGAATT TGTCCTACTT TTTGCTGTAT TTGATGAAGG GAAAAGTTGG 
CACTCAGAAA CAAATGCGTC TTTGACACAG GCTGAGGCCC AGCATGAGCT GCACACCATC 
AATGGCTATG TAAACAGGTC TCTGCCAGGT CTTACTGTGT GTCACAAGAG ATCAGTCTAT 
TGGCATGTGA TTGGAATGGG CACCACCCCC GAAGTGCACT CAATTTTTCT CGAAGGTCAC 
ACATTTCTTG TGAGGAACCA CCGCCAGGCC TCCTTGGAGA TCTCACCAAT TACTTTCCTT 
ACTGCTCAGA CATTCCTGAT GGACCTTGGC CAGTTTCTAC TGTTTTGTCA TATCCCTTCC 
CATCAACATG ATGGTATGGA AGCTTATGTC AAAGTAGATA GCTGCCCAGA GGAACCCCAG 
CTGCGCATGA AAAATAATGA AGATAAAGAT TATGATGATG GTCTTTATGA TTCTGACATG 
GACGTAGTTA GCTTTGATGA CGACAGCTCT TCTCCCTTTA TCCAAATCCG CTCAGTTGCC 
AAGAAGCATC CTAAAACTTG GGTCCACTAT ATTGCTGCTG AGGAGGAGGA CTGGGACTAT 
GCTCCCTCAG GCCCCACCCC CAATGATAGA AGTCATAAAA ATCTGTATTT GAACAATGGT 
CCTCAGCGGA TTGGTAAGAA GTACAAAAAA GTCCGATTTG TGGCATACAC AGATGAGACA 
TTTAAGACTC GTGAAGCTAT TCAGTATGAA TCAGGAATCC TGGGACCTTT ACTTTATGGA 
GAAGTTGGAG ACACACTGCT GATTATATTT AAGAATCAAG CCAGCCGGCC ATATAACATC 
TACCCTCATG GGATCAATTA TGTCACTCCT CTGCACACAG GGAGATTGCC AAAAGGTGTG 
AAACATTTGA AAGATATGCC AATTCTGCCG GGAGAGATAT TCAAGTATAA ATGGACAGTG 
ACCGTAGAAG ATGGACCAAC TAAATCAGAT CCTCGGTGCC TGACCCGATA TTACTCAAGC 
TTCATTAATC TGGAGAGAGA TCTAGCTTCA GGACTCATTG GCCCTCTTCT CATCTGCTAC 
AAAGAATCTG TAGATCAAAG AGGAAACCAG ATGATGTCAG ACAAGAGAAA TGTCATCCTG 
TTTTCTGTAT TTGATGAGAA TCGAAGCTGG TACCTCACAG AGAATATGCA GCGCTTCCTC 
CCCAATGCAG ATGTAGTGCA GCCCCATGAC CCAGAGTTCC AACTCTCTAA CATCATGCAC 
AGCATCAATG GCTATGTTTT TGACAACTTG CAGCTGTCAG TTTGTTTGCA TGAGGTGGCG 
TACTGGTACA TTCTAAGTGT TGGAGCACAA ACTGACTTCC TGTCTGTCTT CTTCTCTGGA 
TATACCTTCA AACACAAAAT GGTCTATGAA GACACACTTA CCCTCTTCCC ATTCTCAGGA 
GAAACTGTCT TCATGTCAAT GGAAAACCCA GGTCTGTGGG TTCTGGGGTG CCACAACTCA 
GACTTTCGGA ACAGAGGCAT GACAGCCTTA CTGAAGGTTT CTAGTTGTAA CAGGAACATT 
GATGATTATT ATGAGGACAC ATACGAAGAT ATTCCAACTC CCCTGCTAAA TGAAAACAAT 
GTAATTAAAC CTAGAAGCTT CTCCCAGAAT TCAAGGCACC CTAGCACTAA GGAAAAGCAA 
TTGAAAGCCA CCACAACTCC AGAAAATGAC ATAGAGAAGA TTGACCTTCA ATCTGGAGAA 
AGAACACAGC TGATTAAAGC ACAAAGTGTC TCCTCTAGTG ATTTGTTGAT GCTGTTGGGA 
CAGAATCCTA CTCCACGTGG ACTGTTCTTA TCTGATCTCC GAGAGGCCAC AGATAGAGCC 
GATGACCATT CACGTGGAGC AATAGAAAGA AACAAGGGCC CACCTGAAGT GGCAAGTCTC 
AGACCAGAGC TCCGTCACAG TGAGGACAGA GAATTTACTC CTGAGCCAGA ACTGCAGTTA 
AGATTAAATG AGAATTTGGG GACAAATACA ACAGTAGAGT TGAAGAAACT TGATTTAAAA 
ATTTCTAGTT CATCAGACAG TCTAATGACT TCACCAACAA TTCCATCAGA TAAGTTGGCA 
GCAGCTACTG AAAAGACAGG TTCCTTAGGA CCCCCAAATA TGTCAGTTCA CTTTAACAGT 
CATTTAGGTA CCATTGTATT TGGCAATAAT TCATCCCACC TTATTCAGTC TGGTGTACCT 
TTGGAATTGA GTGAAGAAGA TAATGATTCC AAGTTGTTAG AAGCACCTTT AATGAATATT 
CAAGAAAGTT CACTGAGAGA AAATGTATTA TCAATGGAGA GTAATAGGTT ATTTAAAGAA 
GAAAGAATTC GTGGACCTGC TTCATTAATC AAAGATAATG CTTTATTCAA AGTTAATATC 
TCTTCGGTAA AGACAAACAG GGCACCAGTT AACTTAACAA CTAATAGAAA GACTCGTGTT 
GCTATCCCAA CATTATTAAT TGAGAACAGT ACCTCAGTCT GGCAAGATAT TATGTTAGAA 
AGGAATACTG AGTTTAAAGA AGTAACTTCT TTGATTCATA ATGAAACGTT TATGGACAGA 
AATACTACAG CTCTGGGGCT AAATCATGTG TCAAATAAAA CTACTTTATC AAAAAATGTG 
GAAATGGCCC ACCAAAAAAA AGAAGACCCT GTGCCACTAC GTGCAGAAAA TCCAGATCTA 
TCATCCTCCA AGATACCGTT CTTGCCAGAT TGGATAAAGA CCCATGGCAA GAACTCCCTA 
AGCTCTGAGC AAAGGCCCAG TCCAAAACAA TTAACATCTT TAGGATCAGA AAAATCTGTG 
AAAGATCAGA ACTTTTTGTC AGAGGAGAAG GTGGTAGTAG GAGAGGATGA ATTTACGAAG 
GACACAGAAC TCCAAGAGAT TTTTCCAAAC AACAAGAGCA TATTTTTTGC TAACTTGGCT 
AATGTCCAAG AAAATGATAC ATACAATCAA GAAAAAAAAT CTCCGGAAGA GATAGAAAGA 
AAGGAAAAAT TAACCCAGGA GAATGTGGCT TTGCCTCAGG CACATACTAT GATTGGCACT 
AAGAACTTCC TGAAGAACCT TTTCTTACTA AGCACTAAGC AAAATGTAGC AGGTTTAGAA 
GAGCAGCCAT ATACTCCAAT ACTTCAAGAC ACCAGGTCAT TAAATGATTC GCCACATAGT 
GAAGGGATTC ATATGGCCAA TTTCTCAAAA ATAAGGGAAG AAGCAAACTT GGAAGGCTTG 
GGAAATCAAA CAAACCAAAT GGTAGAGAGG TTTCCAAGCA CTACGAGGAT GTCTTCTAAT 
GCAAGTCAGC ATGTTATCAC TCAACGTGGT AAGCGGAGTT TGAAACAACC CAGACTCTCA 
CAAGGAGAAA TAAAGTTTGA AAGGAAGGTT ATTGCAAATG ACACTTCAAC CCAGTGGTCC 
AAAAACATGA ACTATTTGGC CCAGGGAACC CTCACACAGA TAGAGTATAA TGAGAAAGAA 
AAAAGGGCCA TTACTCAGTC CCCCCTATCA GATTGTTCTA TGAGGAATCA TGTCACCATT 
CAAATGAATG ACTCTGCATT ACCCGTTGCA AAGGAATCAG CATCTCCATC AGTTAGACAT 
ACAGATCTGA CCAAGATCCC ATCCCAACAC AACTCTTCTC ATCTTCCAGC ATCAGCCTGT 
AATTATACCT TTAGAGAGAG GACTTCTGGA GTCCAAGAAG GCAGTCATTT CTTACAAGAA 
GCCAAAAGAA ATAACCTCTC TTTAGCCTTT GTAACCTTAG GAATAACTGA AGGGCAAGGA 
AAGTTCAGCT CCCTGGGGAA AAGTGCCACA AACCAACCCA TGTACAAGAA ACTTGAAAAC 
ACTGTTCTCT TGCAACCAGG CTTGTCCGAA ACATCTGACA AAGTTGAATT ACTTTCTCAA 
GTTCATGTTG ATCAAGAAGA CTCTTTCCCT ACAAAAACTA GCAATGATTC TCCTGGCCAC 
CTGGATCTCA TGGGAAAGAT CTTCCTTCAG AAAACACAGG GACCTGTTAA AATGAATAAA 
ACAAATAGCC CTGGAAAAGT GCCCTTTCTG AAATGGGCAA CAGAAAGCTC TGAAAAGATT 
CCCTCCAAGC TGCTGGGTGT CCTTGCTTGG GATAACCACT ATGATACCCA GATACCAAGT 
GAAGAGTGGA AATCCCAAAA AAAGTCACAG ACGAACACAG CTTTTAAAAG GAAAGACACC 
ATTTTGCCCC TGGGCCCTTG TGAAAATAAT GATTCAACAG CAGCAATAAA TGAAGGACAA 
GATAAGCCCC AAAGAGAAGC TATGTGGGCA AAGCAAGGAG AGCCTGGAAG GTTGTGCTCT 
CAAAACCCAC CAGTCTCAAA ACACCATCAA AGGGAAATAA CCGTTACTAC TCTTCAGCCA 
GAGGAAGACA AATTTGAGTA TGATGACACC TTCTCAATTG AAATGAAGAG AGAAGATTTT 
GACATCTACG GCGACTATGA AAATCAGGGC CTCCGCAGCT TTCAAAAGAA AACACGACAC 
TATTTCATTG CTGCAGTGGA GCGTCTCTGG GATTATGGGA TGAGTAGATC TCCCCATATA 
CTAAGAAACA GGGCTCAAAG TGGGGATGTC CAGCAGTTCA AGAAGGTGGT TTTCCAGGAA 
TTTACTGATG GATCCTTTAC TCAGCCCTTA TACCGTGGAG AACTGAACGA ACACTTGGGA 
CTCTTGGGGC CATATATAAG AGCAGAAGTT GAAGACAATA TCGTGGTAAC TTTCAAAAAC 
CAGGCCTCTC GTCCCTACTC CTTCTATTCT AGTCTTATTT CTTATGACGA AGATGAGGGA 
CAAGGAGCAG AACCTAGAAG AAAGTTTGTC AACCCTAATG AAACCAAAAT TTACTTTTGG 
AAAGTGCAGC ATCATATGGC ACCCACTAAA GATGAGTTTG ACTGCAAAGC CTGGGCTTAT 
TTTTCTGATG TTGATCTGGA GAAAGATGTG CACTCAGGCT TGATTGGACC CCTTCTGATC 
TGCCGCAGTA ACACACTGAA CCCTGCTCAT GGGAGACAAG TGACAGTGCA GGAGTTTGCC 
CTGGTTTTCA CTATATTCGA TGAGACTAAG AGCTGGTACT TCACTGAAAA CCTGGAAAGG 
AACTGCAGAG CTCCCTGCAA TGTCCAGAAG GAGGACCCTA CTCTAAAAGA AAACTTCCGC 
TTCCATGCAA TCAACGGCTA TGTGAAGGAT ACACTCCCTG GCTTAGTAAT GGCTCAGGAT 
CAAAAGGTTC GATGGTATCT GCTCAGCATG GGCAGCAACG AAAACATTCA TTCCATTCAC 
TTCAGTGGAC ATGTGTTCAC TGTACGGAAA AAAGAGGAAT ATAAAATGGC AGTCTACAAC 
CTCTATCCAG GTGTTTTTGA GACTGTGGAA ATGCTACCAT CCCAAGTTGG AATCTGGCGG 
ATAGAATGCC TTATCGGCGA GCACCTGCAA GCCGGGATGA GCACTCTGTT TCTGGTGTAC 
AGCAAGAAGT GTCAGACTCC ACTGGGGATG GCTTCCGGAC ACATTAGAGA TTTTCAGATT 
ACAGCTTCAG GACAATATGG ACAGTGGGCC CCAAAGCTGG CCAGACTTCA TTATTCCGGA 
TCAATCAATG CCTGGAGCAC CAAGGATCCC TTTTCCTGGA TCAAGGTGGA TCTCTTGGCA 
CCGATGATTA TTCACGGCAT CATGACCCAG GGGGCCCGCC AGAAGTTCTC CAGCCTCTAC 
GTGTCTCAGT TTATCATCAT GTACAGTCTG GATGGCAACA AGTGGCACAG TTACCGAGGG 
AATTCCACGG GGACCTTAAT GGTCTTCTTT GGCAACGTGG ATTCATCTGG GATCAAACAC 
AATATTTTTA ACCCTCCGAT TATTGCTCAG TACATCCGTT TGCACCCAAC CCATTACAGC 
ATCCGCAGCA CTCTTCGCAT GGAGCTCTTG GGCTGTGACT TCAACAGTTG CAGCATGCCG 
CTGGGGATGG AGAGTAAAGC AATATCAGAT GCTCAGATCA CTGCCTCGTC CTACCTAAGC 
AGTATGCTTG CCACTTGGTC TCCTTCCCAA GCCCGGCTGC ACCTGCAGGG CAGGACTAAT 
GCCTGGAGAC CTCAGGCAAA TAACCCAAAA GAGTGGCTGC AAGTGGACTT CCGGAAGACC 
ATGAAAGTCA CAGGAATAAC CACCCAGGGG GTGAAATCTC TCCTCATCAG CATGTATGTG 
AAGGAGTTCC TCATCTCCAG TAGTCAAGAT GGCCATAACT GGACTCTGTT TCTTCAGAAT 
GGCAAAGTCA AGGTCTTCCA GGGAAACCGG GACTCCTCCA CGCCTGTGCG GAACGCTCTC 
GAACCCCCGC TGGTGGCTCG CTACGTGCGC CTGCACCCGC AGAGCTGGGC GCACCACATC 
GCCCTGAGGC TGGAGGTCCT GGGCTGCGAC ACCCAGCAGC CCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022271202.1
CDS	293..7060
Translation	MGLLGPTIQAEVYDTVVIVLKNMASHPVSLHAVGVSYWKASEGAEYEDQTSQKEKEDDNVIPGESHTYVWQVLKENGPMASDPPCLTYSYFSHVDLVKDLNSGLIGALLVCKEGSLAKERTQTLQEFVLLFAVFDEGKSWHSETNASLTQAEAQHELHTINGYVNRSLPGLTVCHKRSVYWHVIGMGTTPEVHSIFLEGHTFLVRNHRQASLEISPITFLTAQTFLMDLGQFLLFCHIPSHQHDGMEAYVKVDSCPEEPQLRMKNNEDKDYDDGLYDSDMDVVSFDDDSSSPFIQIRSVAKKHPKTWVHYIAAEEEDWDYAPSGPTPNDRSHKNLYLNNGPQRIGKKYKKVRFVAYTDETFKTREAIQYESGILGPLLYGEVGDTLLIIFKNQASRPYNIYPHGINYVTPLHTGRLPKGVKHLKDMPILPGEIFKYKWTVTVEDGPTKSDPRCLTRYYSSFINLERDLASGLIGPLLICYKESVDQRGNQMMSDKRNVILFSVFDENRSWYLTENMQRFLPNADVVQPHDPEFQLSNIMHSINGYVFDNLQLSVCLHEVAYWYILSVGAQTDFLSVFFSGYTFKHKMVYEDTLTLFPFSGETVFMSMENPGLWVLGCHNSDFRNRGMTALLKVSSCNRNIDDYYEDTYEDIPTPLLNENNVIKPRSFSQNSRHPSTKEKQLKATTTPENDIEKIDLQSGERTQLIKAQSVSSSDLLMLLGQNPTPRGLFLSDLREATDRADDHSRGAIERNKGPPEVASLRPELRHSEDREFTPEPELQLRLNENLGTNTTVELKKLDLKISSSSDSLMTSPTIPSDKLAAATEKTGSLGPPNMSVHFNSHLGTIVFGNNSSHLIQSGVPLELSEEDNDSKLLEAPLMNIQESSLRENVLSMESNRLFKEERIRGPASLIKDNALFKVNISSVKTNRAPVNLTTNRKTRVAIPTLLIENSTSVWQDIMLERNTEFKEVTSLIHNETFMDRNTTALGLNHVSNKTTLSKNVEMAHQKKEDPVPLRAENPDLSSSKIPFLPDWIKTHGKNSLSSEQRPSPKQLTSLGSEKSVKDQNFLSEEKVVVGEDEFTKDTELQEIFPNNKSIFFANLANVQENDTYNQEKKSPEEIERKEKLTQENVALPQAHTMIGTKNFLKNLFLLSTKQNVAGLEEQPYTPILQDTRSLNDSPHSEGIHMANFSKIREEANLEGLGNQTNQMVERFPSTTRMSSNASQHVITQRGKRSLKQPRLSQGEIKFERKVIANDTSTQWSKNMNYLAQGTLTQIEYNEKEKRAITQSPLSDCSMRNHVTIQMNDSALPVAKESASPSVRHTDLTKIPSQHNSSHLPASACNYTFRERTSGVQEGSHFLQEAKRNNLSLAFVTLGITEGQGKFSSLGKSATNQPMYKKLENTVLLQPGLSETSDKVELLSQVHVDQEDSFPTKTSNDSPGHLDLMGKIFLQKTQGPVKMNKTNSPGKVPFLKWATESSEKIPSKLLGVLAWDNHYDTQIPSEEWKSQKKSQTNTAFKRKDTILPLGPCENNDSTAAINEGQDKPQREAMWAKQGEPGRLCSQNPPVSKHHQREITVTTLQPEEDKFEYDDTFSIEMKREDFDIYGDYENQGLRSFQKKTRHYFIAAVERLWDYGMSRSPHILRNRAQSGDVQQFKKVVFQEFTDGSFTQPLYRGELNEHLGLLGPYIRAEVEDNIVVTFKNQASRPYSFYSSLISYDEDEGQGAEPRRKFVNPNETKIYFWKVQHHMAPTKDEFDCKAWAYFSDVDLEKDVHSGLIGPLLICRSNTLNPAHGRQVTVQEFALVFTIFDETKSWYFTENLERNCRAPCNVQKEDPTLKENFRFHAINGYVKDTLPGLVMAQDQKVRWYLLSMGSNENIHSIHFSGHVFTVRKKEEYKMAVYNLYPGVFETVEMLPSQVGIWRIECLIGEHLQAGMSTLFLVYSKKCQTPLGMASGHIRDFQITASGQYGQWAPKLARLHYSGSINAWSTKDPFSWIKVDLLAPMIIHGIMTQGARQKFSSLYVSQFIIMYSLDGNKWHSYRGNSTGTLMVFFGNVDSSGIKHNIFNPPIIAQYIRLHPTHYSIRSTLRMELLGCDFNSCSMPLGMESKAISDAQITASSYLSSMLATWSPSQARLHLQGRTNAWRPQANNPKEWLQVDFRKTMKVTGITTQGVKSLLISMYVKEFLISSSQDGHNWTLFLQNGKVKVFQGNRDSSTPVRNALEPPLVARYVRLHPQSWAHHIALRLEVLGCDTQQPA

Target ORF information:

RefSeq Version	XM_022415494.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris coagulation factor VIII (F8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022415494.1

ORF Insert Sequence:

ATGGGCCTGC TGGGTCCTAC CATCCAGGCT GAGGTTTATG ACACAGTGGT CATTGTCCTT 
AAGAACATGG CTTCTCATCC TGTCAGCCTT CACGCTGTTG GTGTATCCTA TTGGAAAGCT 
TCTGAAGGTG CTGAGTATGA GGATCAGACC AGCCAAAAGG AGAAGGAAGA TGATAATGTC 
ATTCCTGGTG AAAGCCATAC CTATGTCTGG CAGGTCCTGA AAGAGAATGG CCCAATGGCC 
TCTGATCCAC CATGTCTCAC CTACTCATAT TTTTCACACG TGGACCTGGT GAAAGACCTG 
AATTCAGGCC TCATTGGAGC CCTGCTGGTT TGCAAAGAAG GGAGTCTGGC CAAAGAAAGG 
ACACAGACCT TGCAGGAATT TGTCCTACTT TTTGCTGTAT TTGATGAAGG GAAAAGTTGG 
CACTCAGAAA CAAATGCGTC TTTGACACAG GCTGAGGCCC AGCATGAGCT GCACACCATC 
AATGGCTATG TAAACAGGTC TCTGCCAGGT CTTACTGTGT GTCACAAGAG ATCAGTCTAT 
TGGCATGTGA TTGGAATGGG CACCACCCCC GAAGTGCACT CAATTTTTCT CGAAGGTCAC 
ACATTTCTTG TGAGGAACCA CCGCCAGGCC TCCTTGGAGA TCTCACCAAT TACTTTCCTT 
ACTGCTCAGA CATTCCTGAT GGACCTTGGC CAGTTTCTAC TGTTTTGTCA TATCCCTTCC 
CATCAACATG ATGGTATGGA AGCTTATGTC AAAGTAGATA GCTGCCCAGA GGAACCCCAG 
CTGCGCATGA AAAATAATGA AGATAAAGAT TATGATGATG GTCTTTATGA TTCTGACATG 
GACGTAGTTA GCTTTGATGA CGACAGCTCT TCTCCCTTTA TCCAAATCCG CTCAGTTGCC 
AAGAAGCATC CTAAAACTTG GGTCCACTAT ATTGCTGCTG AGGAGGAGGA CTGGGACTAT 
GCTCCCTCAG GCCCCACCCC CAATGATAGA AGTCATAAAA ATCTGTATTT GAACAATGGT 
CCTCAGCGGA TTGGTAAGAA GTACAAAAAA GTCCGATTTG TGGCATACAC AGATGAGACA 
TTTAAGACTC GTGAAGCTAT TCAGTATGAA TCAGGAATCC TGGGACCTTT ACTTTATGGA 
GAAGTTGGAG ACACACTGCT GATTATATTT AAGAATCAAG CCAGCCGGCC ATATAACATC 
TACCCTCATG GGATCAATTA TGTCACTCCT CTGCACACAG GGAGATTGCC AAAAGGTGTG 
AAACATTTGA AAGATATGCC AATTCTGCCG GGAGAGATAT TCAAGTATAA ATGGACAGTG 
ACCGTAGAAG ATGGACCAAC TAAATCAGAT CCTCGGTGCC TGACCCGATA TTACTCAAGC 
TTCATTAATC TGGAGAGAGA TCTAGCTTCA GGACTCATTG GCCCTCTTCT CATCTGCTAC 
AAAGAATCTG TAGATCAAAG AGGAAACCAG ATGATGTCAG ACAAGAGAAA TGTCATCCTG 
TTTTCTGTAT TTGATGAGAA TCGAAGCTGG TACCTCACAG AGAATATGCA GCGCTTCCTC 
CCCAATGCAG ATGTAGTGCA GCCCCATGAC CCAGAGTTCC AACTCTCTAA CATCATGCAC 
AGCATCAATG GCTATGTTTT TGACAACTTG CAGCTGTCAG TTTGTTTGCA TGAGGTGGCG 
TACTGGTACA TTCTAAGTGT TGGAGCACAA ACTGACTTCC TGTCTGTCTT CTTCTCTGGA 
TATACCTTCA AACACAAAAT GGTCTATGAA GACACACTTA CCCTCTTCCC ATTCTCAGGA 
GAAACTGTCT TCATGTCAAT GGAAAACCCA GGTCTGTGGG TTCTGGGGTG CCACAACTCA 
GACTTTCGGA ACAGAGGCAT GACAGCCTTA CTGAAGGTTT CTAGTTGTAA CAGGAACATT 
GATGATTATT ATGAGGACAC ATACGAAGAT ATTCCAACTC CCCTGCTAAA TGAAAACAAT 
GTAATTAAAC CTAGAAGCTT CTCCCAGAAT TCAAGGCACC CTAGCACTAA GGAAAAGCAA 
TTGAAAGCCA CCACAACTCC AGAAAATGAC ATAGAGAAGA TTGACCTTCA ATCTGGAGAA 
AGAACACAGC TGATTAAAGC ACAAAGTGTC TCCTCTAGTG ATTTGTTGAT GCTGTTGGGA 
CAGAATCCTA CTCCACGTGG ACTGTTCTTA TCTGATCTCC GAGAGGCCAC AGATAGAGCC 
GATGACCATT CACGTGGAGC AATAGAAAGA AACAAGGGCC CACCTGAAGT GGCAAGTCTC 
AGACCAGAGC TCCGTCACAG TGAGGACAGA GAATTTACTC CTGAGCCAGA ACTGCAGTTA 
AGATTAAATG AGAATTTGGG GACAAATACA ACAGTAGAGT TGAAGAAACT TGATTTAAAA 
ATTTCTAGTT CATCAGACAG TCTAATGACT TCACCAACAA TTCCATCAGA TAAGTTGGCA 
GCAGCTACTG AAAAGACAGG TTCCTTAGGA CCCCCAAATA TGTCAGTTCA CTTTAACAGT 
CATTTAGGTA CCATTGTATT TGGCAATAAT TCATCCCACC TTATTCAGTC TGGTGTACCT 
TTGGAATTGA GTGAAGAAGA TAATGATTCC AAGTTGTTAG AAGCACCTTT AATGAATATT 
CAAGAAAGTT CACTGAGAGA AAATGTATTA TCAATGGAGA GTAATAGGTT ATTTAAAGAA 
GAAAGAATTC GTGGACCTGC TTCATTAATC AAAGATAATG CTTTATTCAA AGTTAATATC 
TCTTCGGTAA AGACAAACAG GGCACCAGTT AACTTAACAA CTAATAGAAA GACTCGTGTT 
GCTATCCCAA CATTATTAAT TGAGAACAGT ACCTCAGTCT GGCAAGATAT TATGTTAGAA 
AGGAATACTG AGTTTAAAGA AGTAACTTCT TTGATTCATA ATGAAACGTT TATGGACAGA 
AATACTACAG CTCTGGGGCT AAATCATGTG TCAAATAAAA CTACTTTATC AAAAAATGTG 
GAAATGGCCC ACCAAAAAAA AGAAGACCCT GTGCCACTAC GTGCAGAAAA TCCAGATCTA 
TCATCCTCCA AGATACCGTT CTTGCCAGAT TGGATAAAGA CCCATGGCAA GAACTCCCTA 
AGCTCTGAGC AAAGGCCCAG TCCAAAACAA TTAACATCTT TAGGATCAGA AAAATCTGTG 
AAAGATCAGA ACTTTTTGTC AGAGGAGAAG GTGGTAGTAG GAGAGGATGA ATTTACGAAG 
GACACAGAAC TCCAAGAGAT TTTTCCAAAC AACAAGAGCA TATTTTTTGC TAACTTGGCT 
AATGTCCAAG AAAATGATAC ATACAATCAA GAAAAAAAAT CTCCGGAAGA GATAGAAAGA 
AAGGAAAAAT TAACCCAGGA GAATGTGGCT TTGCCTCAGG CACATACTAT GATTGGCACT 
AAGAACTTCC TGAAGAACCT TTTCTTACTA AGCACTAAGC AAAATGTAGC AGGTTTAGAA 
GAGCAGCCAT ATACTCCAAT ACTTCAAGAC ACCAGGTCAT TAAATGATTC GCCACATAGT 
GAAGGGATTC ATATGGCCAA TTTCTCAAAA ATAAGGGAAG AAGCAAACTT GGAAGGCTTG 
GGAAATCAAA CAAACCAAAT GGTAGAGAGG TTTCCAAGCA CTACGAGGAT GTCTTCTAAT 
GCAAGTCAGC ATGTTATCAC TCAACGTGGT AAGCGGAGTT TGAAACAACC CAGACTCTCA 
CAAGGAGAAA TAAAGTTTGA AAGGAAGGTT ATTGCAAATG ACACTTCAAC CCAGTGGTCC 
AAAAACATGA ACTATTTGGC CCAGGGAACC CTCACACAGA TAGAGTATAA TGAGAAAGAA 
AAAAGGGCCA TTACTCAGTC CCCCCTATCA GATTGTTCTA TGAGGAATCA TGTCACCATT 
CAAATGAATG ACTCTGCATT ACCCGTTGCA AAGGAATCAG CATCTCCATC AGTTAGACAT 
ACAGATCTGA CCAAGATCCC ATCCCAACAC AACTCTTCTC ATCTTCCAGC ATCAGCCTGT 
AATTATACCT TTAGAGAGAG GACTTCTGGA GTCCAAGAAG GCAGTCATTT CTTACAAGAA 
GCCAAAAGAA ATAACCTCTC TTTAGCCTTT GTAACCTTAG GAATAACTGA AGGGCAAGGA 
AAGTTCAGCT CCCTGGGGAA AAGTGCCACA AACCAACCCA TGTACAAGAA ACTTGAAAAC 
ACTGTTCTCT TGCAACCAGG CTTGTCCGAA ACATCTGACA AAGTTGAATT ACTTTCTCAA 
GTTCATGTTG ATCAAGAAGA CTCTTTCCCT ACAAAAACTA GCAATGATTC TCCTGGCCAC 
CTGGATCTCA TGGGAAAGAT CTTCCTTCAG AAAACACAGG GACCTGTTAA AATGAATAAA 
ACAAATAGCC CTGGAAAAGT GCCCTTTCTG AAATGGGCAA CAGAAAGCTC TGAAAAGATT 
CCCTCCAAGC TGCTGGGTGT CCTTGCTTGG GATAACCACT ATGATACCCA GATACCAAGT 
GAAGAGTGGA AATCCCAAAA AAAGTCACAG ACGAACACAG CTTTTAAAAG GAAAGACACC 
ATTTTGCCCC TGGGCCCTTG TGAAAATAAT GATTCAACAG CAGCAATAAA TGAAGGACAA 
GATAAGCCCC AAAGAGAAGC TATGTGGGCA AAGCAAGGAG AGCCTGGAAG GTTGTGCTCT 
CAAAACCCAC CAGTCTCAAA ACACCATCAA AGGGAAATAA CCGTTACTAC TCTTCAGCCA 
GAGGAAGACA AATTTGAGTA TGATGACACC TTCTCAATTG AAATGAAGAG AGAAGATTTT 
GACATCTACG GCGACTATGA AAATCAGGGC CTCCGCAGCT TTCAAAAGAA AACACGACAC 
TATTTCATTG CTGCAGTGGA GCGTCTCTGG GATTATGGGA TGAGTAGATC TCCCCATATA 
CTAAGAAACA GGGCTCAAAG TGGGGATGTC CAGCAGTTCA AGAAGGTGGT TTTCCAGGAA 
TTTACTGATG GATCCTTTAC TCAGCCCTTA TACCGTGGAG AACTGAACGA ACACTTGGGA 
CTCTTGGGGC CATATATAAG AGCAGAAGTT GAAGACAATA TCGTGGTAAC TTTCAAAAAC 
CAGGCCTCTC GTCCCTACTC CTTCTATTCT AGTCTTATTT CTTATGACGA AGATGAGGGA 
CAAGGAGCAG AACCTAGAAG AAAGTTTGTC AACCCTAATG AAACCAAAAT TTACTTTTGG 
AAAGTGCAGC ATCATATGGC ACCCACTAAA GATGAGTTTG ACTGCAAAGC CTGGGCTTAT 
TTTTCTGATG TTGATCTGGA GAAAGATGTG CACTCAGGCT TGATTGGACC CCTTCTGATC 
TGCCGCAGTA ACACACTGAA CCCTGCTCAT GGGAGACAAG TGACAGTGCA GGAGTTTGCC 
CTGGTTTTCA CTATATTCGA TGAGACTAAG AGCTGGTACT TCACTGAAAA CCTGGAAAGG 
AACTGCAGAG CTCCCTGCAA TGTCCAGAAG GAGGACCCTA CTCTAAAAGA AAACTTCCGC 
TTCCATGCAA TCAACGGCTA TGTGAAGGAT ACACTCCCTG GCTTAGTAAT GGCTCAGGAT 
CAAAAGGTTC GATGGTATCT GCTCAGCATG GGCAGCAACG AAAACATTCA TTCCATTCAC 
TTCAGTGGAC ATGTGTTCAC TGTACGGAAA AAAGAGGAAT ATAAAATGGC AGTCTACAAC 
CTCTATCCAG GTGTTTTTGA GACTGTGGAA ATGCTACCAT CCCAAGTTGG AATCTGGCGG 
ATAGAATGCC TTATCGGCGA GCACCTGCAA GCCGGGATGA GCACTCTGTT TCTGGTGTAC 
AGCAAGAAGT GTCAGACTCC ACTGGGGATG GCTTCCGGAC ACATTAGAGA TTTTCAGATT 
ACAGCTTCAG GACAATATGG ACAGTGGGCC CCAAAGCTGG CCAGACTTCA TTATTCCGGA 
TCAATCAATG CCTGGAGCAC CAAGGATCCC TTTTCCTGGA TCAAGGTGGA TCTCTTGGCA 
CCGATGATTA TTCACGGCAT CATGACCCAG GGGGCCCGCC AGAAGTTCTC CAGCCTCTAC 
GTGTCTCAGT TTATCATCAT GTACAGTCTG GATGGCAACA AGTGGCACAG TTACCGAGGG 
AATTCCACGG GGACCTTAAT GGTCTTCTTT GGCAACGTGG ATTCATCTGG GATCAAACAC 
AATATTTTTA ACCCTCCGAT TATTGCTCAG TACATCCGTT TGCACCCAAC CCATTACAGC 
ATCCGCAGCA CTCTTCGCAT GGAGCTCTTG GGCTGTGACT TCAACAGTTG CAGCATGCCG 
CTGGGGATGG AGAGTAAAGC AATATCAGAT GCTCAGATCA CTGCCTCGTC CTACCTAAGC 
AGTATGCTTG CCACTTGGTC TCCTTCCCAA GCCCGGCTGC ACCTGCAGGG CAGGACTAAT 
GCCTGGAGAC CTCAGGCAAA TAACCCAAAA GAGTGGCTGC AAGTGGACTT CCGGAAGACC 
ATGAAAGTCA CAGGAATAAC CACCCAGGGG GTGAAATCTC TCCTCATCAG CATGTATGTG 
AAGGAGTTCC TCATCTCCAG TAGTCAAGAT GGCCATAACT GGACTCTGTT TCTTCAGAAT 
GGCAAAGTCA AGGTCTTCCA GGGAAACCGG GACTCCTCCA CGCCTGTGCG GAACGCTCTC 
GAACCCCCGC TGGTGGCTCG CTACGTGCGC CTGCACCCGC AGAGCTGGGC GCACCACATC 
GCCCTGAGGC TGGAGGTCCT GGGCTGCGAC ACCCAGCAGC CCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Omental implantation of BOECs in hemophilia dogs results in circulating FVIII antigen and a complex immune response.
Blood123(26)4045-53(2014 Jun)
Ozelo MC,Vidal B,Brown C,Notley C,Hegadorn C,Webster S,Harpell L,Ahlin J,Winterborn A,Handforth J,Arruda VR,Hough C,Lillicrap D

Minimal modification in the factor VIII B-domain sequence ameliorates the murine hemophilia A phenotype.
Blood121(21)4396-403(2013 May)
Siner JI,Iacobelli NP,Sabatino DE,Ivanciu L,Zhou S,Poncz M,Camire RM,Arruda VR

The canine factor VIII cDNA and 5' flanking sequence.
Thrombosis and haemostasis79(2)317-22(1998 Feb)
Cameron C,Notley C,Hoyle S,McGlynn L,Hough C,Kamisue S,Giles A,Lillicrap D

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F8 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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