F8 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	F8
Entrez Gene ID	100271720
Full Name	coagulation factor VIII
General protein information	Preferred Names coagulation factor VIII Names coagulation factor VIII coagulation factor VIII, procoagulant component factor VIII
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005227639.3	XP_005227696.1	coagulation factor VIII isoform X1
NM_001145508.1	NP_001138980.1	coagulation factor VIII precursor
XM_024987721.1	XP_024843489.1	coagulation factor VIII isoform X5
XM_024987718.1	XP_024843486.1	coagulation factor VIII isoform X2
XM_005227639.4	XP_005227696.1	coagulation factor VIII isoform X1
XM_024987719.1	XP_024843487.1	coagulation factor VIII isoform X3
XM_024987720.1	XP_024843488.1	coagulation factor VIII isoform X4
XM_024987717.1	XP_024843485.1	coagulation factor VIII isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	F8	NP_000123.1
Canis lupus familiaris (dog)	F8	NP_001003212.1
Pan troglodytes (chimpanzee)	F8	XP_003317837.1
Rattus norvegicus (Norway rat)	F8	NP_899160.1
Macaca mulatta (Rhesus monkey)	LOC100424151	XP_002806506.1
Mus musculus (house mouse)	F8	NP_032003.2
Bos taurus (cattle)	F8	NP_001138980.1

Gene Ontology
Bibliography

Related articles in PubMed

A mild form of haemophilia A is associated with two factor VIII missense mutations in German Fleckvieh cattle.
Reinartz S, Weiß C, Mischke R, Distl O
Animal genetics49(4)350-351(2018 Aug)

Importance of a factor VIIIc-like glycoprotein expressed in capillary endothelial cells (eFactor VIIIc) in angiogenesis.
Banerjee DK, Oliveira CM, Tavárez JJ, Katiyar VN, Saha S, Martínez JA, Banerjee A, Sánchez A, Baksi K
Advances in experimental medicine and biology705453-64(2011)

A missense mutation (p.Leu2153His) of the factor VIII gene causes cattle haemophilia A.
Khalaj M, Abbasi AR, Shimojo K, Moritomo Y, Yoneda K, Kunieda T
Animal genetics40(5)763-5(2009 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following F8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the F8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa238234	XM_024987721.1 Latest version!	Bos taurus coagulation factor VIII (F8), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OBa39845	NM_001145508.1 Latest version!	Bos taurus coagulation factor VIII (F8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa238231	XM_024987718.1 Latest version!	Bos taurus coagulation factor VIII (F8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa238232	XM_024987719.1 Latest version!	Bos taurus coagulation factor VIII (F8), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa238233	XM_024987720.1 Latest version!	Bos taurus coagulation factor VIII (F8), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa35243	XM_024987717.1 Latest version!	Bos taurus coagulation factor VIII (F8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa35243	XM_005227639.4 Latest version!	Bos taurus coagulation factor VIII (F8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OBa35243	XM_005227639.3	Bos taurus coagulation factor VIII (F8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

F8 ( XM_024987721.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024843489.1 Bos taurus coagulation factor VIII (F8), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa238234
Clone ID Related Accession (Same CDS sequence)	XM_024987721.1
Accession Version	XM_024987721.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 765bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	coagulation factor VIII isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTATTC ACAGCATCCT GACTCAGGGT GCCCGGCAGA AGTTCTCCAG CCTGTACATC 
TCTCAGTTTA TCATCATGTA CAGCCTCGAT GGACAGCGGT GGCAGGGTTA TCGGGGGAAC 
TCCACTGGGA CCTTAATGGT GTTCTTTGGT AATGTGGATT CATCTGGAGT AAAACACAAT 
ATTTTTAACC CTCCAATTAT TGCTAGATAT ATCCGTTTAC ACCCAACTCA TTACAGCATC 
CGCAGCACTC TTCGCATGGA GTTGATGGGC TGTGATTTAA ACAGTTGCAA CATGCCACTG 
GGAATGGAGA ATAAAGCTAT ATCAGATACA CAGATTACTG CCTCATCCCA CTTACGCAAC 
ATGTTTGCCA CCTGGTCTCC TTCACAAGCC CGACTTAACC TTCAAGGGAG GACAAATGCC 
TGGAGGCCCC AGGTGAATAA TCCAAAAGAG TGGCTACAAG TGGACTTCCA GAAGACAATG 
AGAGTTACAG GAATAACTAC CCAAGGGGTG AAATCTCTGC TTACCAGCAT GTATGTGAAG 
GAGTTCCTCA TATCCAGTAG TCAAGATGGC CATAACTGGA CTCCATTTCT TCAGAATGGC 
AAAGTGAAGG TTTTTCAGGG AAATCAAGAC TCCTTCACCC CCGTGGTGAA TGCTCTAGAC 
CCCCCGCTGT TTACCCGCTT CCTTCGGATT CACCCGCGGA GCTGGGCGCA CCATATCGCC 
CTGAGGCTGG AGCTTTTGGG CTGCGAGGCA CAGCAGCAGT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024843489.1
CDS	135..899
Translation	MIIHSILTQGARQKFSSLYISQFIIMYSLDGQRWQGYRGNSTGTLMVFFGNVDSSGVKHNIFNPPIIARYIRLHPTHYSIRSTLRMELMGCDLNSCNMPLGMENKAISDTQITASSHLRNMFATWSPSQARLNLQGRTNAWRPQVNNPKEWLQVDFQKTMRVTGITTQGVKSLLTSMYVKEFLISSSQDGHNWTPFLQNGKVKVFQGNQDSFTPVVNALDPPLFTRFLRIHPRSWAHHIALRLELLGCEAQQQY

Target ORF information:

RefSeq Version	XM_024987721.1
Organism	Bos taurus(cattle)
Definition	Bos taurus coagulation factor VIII (F8), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024987721.1

ORF Insert Sequence:

ATGATTATTC ACAGCATCCT GACTCAGGGT GCCCGGCAGA AGTTCTCCAG CCTGTACATC 
TCTCAGTTTA TCATCATGTA CAGCCTCGAT GGACAGCGGT GGCAGGGTTA TCGGGGGAAC 
TCCACTGGGA CCTTAATGGT GTTCTTTGGT AATGTGGATT CATCTGGAGT AAAACACAAT 
ATTTTTAACC CTCCAATTAT TGCTAGATAT ATCCGTTTAC ACCCAACTCA TTACAGCATC 
CGCAGCACTC TTCGCATGGA GTTGATGGGC TGTGATTTAA ACAGTTGCAA CATGCCACTG 
GGAATGGAGA ATAAAGCTAT ATCAGATACA CAGATTACTG CCTCATCCCA CTTACGCAAC 
ATGTTTGCCA CCTGGTCTCC TTCACAAGCC CGACTTAACC TTCAAGGGAG GACAAATGCC 
TGGAGGCCCC AGGTGAATAA TCCAAAAGAG TGGCTACAAG TGGACTTCCA GAAGACAATG 
AGAGTTACAG GAATAACTAC CCAAGGGGTG AAATCTCTGC TTACCAGCAT GTATGTGAAG 
GAGTTCCTCA TATCCAGTAG TCAAGATGGC CATAACTGGA CTCCATTTCT TCAGAATGGC 
AAAGTGAAGG TTTTTCAGGG AAATCAAGAC TCCTTCACCC CCGTGGTGAA TGCTCTAGAC 
CCCCCGCTGT TTACCCGCTT CCTTCGGATT CACCCGCGGA GCTGGGCGCA CCATATCGCC 
CTGAGGCTGG AGCTTTTGGG CTGCGAGGCA CAGCAGCAGT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

F8 ( NM_001145508.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001138980.1 Bos taurus coagulation factor VIII (F8), mRNA.

Price & Availability↑

CloneID	OBa39845
Clone ID Related Accession (Same CDS sequence)	NM_001145508.1
Accession Version	NM_001145508.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1541174400000
Organism	Bos taurus(cattle)
Product	coagulation factor VIII precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB485748.1. ##Evidence-Data-START## Transcript exon combination :: AB485748.1, AB485749.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145477, SAMN04019641 [ECO:0000348] ##Evidence-Data-END##

ATGCACATCA AGCTCTCTAC CTGCTTCTTT CTGTGCCTCT GGCCATGCAG CTTCAGTGCC 
ATCAGAAGAT ACTACCTGGG TGCAGTGGAA CTCTCCTGGG ACTATATGCA AAGTGAACTG 
CTCAGTGAGC TGCATGTGAA CACAAGGTTT CCTCCCAGAG TGCCCAAACC TTTTCCATTC 
AACACCTCAG TCATGTACAG AAAGACTGTG TTTGTAGAGT TCACGGATCA ACTTTTCAAC 
ATCGCCAAGC CCAGGCCACC ATGGATGGGT CTGCTGGGTC CAACTATCCA GGCTGAGGTT 
TATGACACCG TGGTCATTAC ATTTAAGAAT ATGGCTTCTC ATCCTGTTAG TCTTCATGCT 
ATTGGCGTAT CCTACTGGAA ATCTTCTGAA GGTGCTGCAT ATGAGGATGA AACCAGCCAA 
AGGGAGAAGG AAGATGACAA AGTCATTCCT GGTAAAAGTC ATACCTACGT CTGGCAGATC 
CTGAAAGAAA ATGGTCCAAC AGCCTCTGAC CCACCATGTC TCACCTACTC ATATCTTTCT 
CATGTGGACC TGGTGAAAGA CGTGAACTCA GGTCTCATTG GAGCCCTGCT AATTTGTAGG 
GAAGGAACTC TGATCAAAGA AAGGACACAG ACCTTGCACA AATTCGTACT ACTGTTTGCT 
GTATTTGATG AAGGGAAAAG TTGGCACTCG GGAAAAAATG AGTCCTTGAC ACGTGTTATG 
GATTCTGCAT CTGCACTGCA CACCATCAAT GGCTATATAA ACAGGTCTCT GCCAGGTCTG 
ATTGGATGTC ATAAGAAATC AGTCTATTGG CACGTGATTG GAATGGGCAC CACCCCGGAA 
GTGCACTCAA TTTTCCTTGA AGGCCACACA TTTCTCGTGA GGAACCATCG CCAGGCTTCC 
TTGGAGATCT CACCAATAAC TTTCCTTACC GCTCAGACAG TCCTGATGGA CTGTGGCCAG 
TTTCTACTGT TTTGTCGTAT CTCTTCCCAC CAACATGATG GTATGGAAGC TTATGTCAAA 
GTAGATAGTT GCCCAGAGGA ACCCCGACTA TGGATGAAAA ATAACCAAGA AGAAGATTAT 
GATGATGGTT TGGATGACTC TGACATGGAT GTGGTCAGGT TTGATGGCGA CAGTGCGCCT 
CCCTTTATCC AAATGCGCTC AGTTGCAAAG AAGCATCCTA AAACCTGGGT CCACTACATT 
GCTGCTGAAG AGGATGACTG GGACTATGCC CCCTCGGTCC TCACCACCAG TGACAGAAGT 
TATAAAAGTC TGTATCTGAA CCACGGTCCT CAGCGGATTG GTAGGAAGTA CAGAAAAGTA 
CGGTTTATAG CTTACACAGA TGGAACATTT AAGACTCGTG AAGCTATTCA GCATGAATCA 
GGGATCCTGG GGCCTTTACT TTATGGAGAA GTTGGAGACA CACTTTTGAT TATATTTAAG 
AATCAAGCAA GCCGGCCATA TAACATCTAC CCTCATGGAA TCACTGATGT CAGTCCTTTG 
CACTCAGGGA GATTTCCAAA AGGTGTGAAA CATTTGAAAG ACATGCCAAT TCTGCCAGGA 
GAAGTGTTCA AGTATAAATG GACAGTGACT GTAGAAGATG GGCCAACTAA ATCAGATCCT 
CGGTGTCTGA CCCGATATTA CTCGAGTTTC ATTAACTTAG AGAAAGATCT AGCTTCAGGA 
CTCATTGGCC CCCTCCTCAT CTGCTACAAA GAATCTGTAG ATCAAAGAGG AAACCAGATG 
ATGTCAGACA AGAGAAATGT CATCCTGTTT TCTGTATTTG ATGAGAATAA AAGTTGGTAC 
CTCACAGAGA ATATTCAGCG CTTCCTCCCC AATGGAGTAC AGCCCCAGGA TCCAGAGTTC 
CAAGTCTCCA ATGTCATGCA TAGCATCAAT GGCTATGTTT TTGATAGCTT GCAGCTGTCG 
GTTTGTTTGC ATGAGGTGGC ATACTGGTAC ATTCTAAGTG TTGGAGCCCA AATTGACTTC 
CTCTCTGTCT TCTTCTCTGG ATATACCTTC AAACACAAAA TGGTCTATGA AGATACACTC 
ACCCTATTCC CCTTTTCAGG AGAAACTGTC TTCATGTCAA TGGAAAATCC AGGTCTGTGG 
GTTCTGGGGT GCCACAACTC AGACTTTCGA AACAGAGGCA TGACAGCCTT ACTGAAGGTT 
GATAGTTGTG ACAGGAACGT TGGCGATTAT TATGACACAT ATGAAGATAT TCCAACCTTG 
CTGAGTGACG ACAATGTCAT TGAACCCAGA AGCTTCTCCA AGAATTCAAG GTACCCTAGC 
ACTAGGCAAA AGCAATTCAA ACCCACCATG ACTCCAGAAA ATGACATGGA GAAGATTGAC 
CCTCAGTCTG GAGAGAAAAT GCAGCTGCTT ACAGAACAAA GTGTCTCCTC TAGTGATCTG 
TTGATGCTCT TGGGACAGAA TCCTACTCCA CACAGATTAT CCTTATCTGA TCTCCAAGAA 
ACCAGAAATG AGGCTACTGA TCATTTACCT GGACCAACAG AAAGAATCAA GGGCCCATCC 
CCATCTGAAG TGGCACATCT TAGACCAGAG CTGCATCACA GTGGGGAAAG AGTATTTACT 
CAAGAGCCAG AACTGCCAGT AAGATTAAAT GAGAATTTGG GAACAACTAT AATGGCAGAG 
TTGAACAAAC TTAATTTAAA AATTTCAAGT TCATCAAACA ATCTAATGCC TTTACCAACA 
ATTCCATCAG ACAAGTTGTC AGCAGGTACT AAAAAGACAG GTTCCTTCGG ACCCCCAAAT 
ATGCCAGTTA ATTTTAATAG TCAATTAGGT GCCATTGTAT TTGGCAAAAA TTTATCTCAT 
GTTATTGGAT CACGTGTACC TTTGGGCTTG AGTAAAGAAG ATAATAATTC CAAGTTGTTA 
GAAGCAGCTT TAATGAATAG TCAAGAAAGT TCACTGGGAG AAAATGCATT ATCACTGGAG 
AGTGATAGGC TATTTAAAGA GGAAAGAGTT CATGGACCTG TGTCATTGAA CAAAGATGAT 
GCTTTATTTA AAGTGAATAT CTCTTTGGTA AAGACAGACA AGGCACCAGC TAACTCAACA 
ACTAATAGAA AGACTCACAT TGATGACCCA ACAGTATTAA TTGAGAATAG TACATCAGTC 
TGGCAGGATA TTGTAATAGA CAGTAATACC AACTTTCAAG AAGTGACTTC TTTGATTCAT 
GATGAATTGT TTATGCACAA AAATACTACA TCTTTTGGAC TAAGCCATAT GTCAAATAAA 
ACTACTTCAT CCAAAAAAAG GGAAATGATC CACCAAGAAA AAGAAGGCCC TAAGCCACTA 
GATACAAAAT ATCCAGATAC ACCATTCTTC AAGACTCTGT TTTTGCCAGA TTCAACAAAC 
GTGATAAAAA GGACCCACGA CAAGAACTCC CTAAGCTCTG GGCAAAGGCC CAGTCCAAAG 
CAATTAACAT CATCAGTATC AGAAAACTCT GTGAAAGATC AGAATGTCTT ATCAGAAAAG 
AATAAGGCAG TAGCAGGAGA GGATGAATTT AGCAACAACA CCAGCCTCAA AGAGATGATT 
TTTCCAAATA CCAAAAGCAT AGTTCTTACT AACTTGGCTA ATGTCCAAGA AAATGATACA 
GACAATCAAG GAAAAAAATC CCAGGAAGAA ACAGAAAAGA AAGAGACATT AATCCCTGAG 
AACACAGTTT TGCCTCAGGT GTATACAGTG ATTGGAACTA AGAATTTCCT GAAGAATCTC 
TTTTCACTGA GCACTAAGCA AAATGTCAGA GGTTTAGATG AAGAGACATA TACTCCAGGA 
CTTCAAGACA ACAGGTCATT AAGTGATTCG GCAAAAAGAG CCAGGATTCA TGCAGCCCAT 
TTCTCAAAAG GAAGGGTGGA AGCAAACGTG GAAGGCTTAG GGAATCAAAC AAAACAAATG 
GTAGAGAAAT CCCCAAGCAC TACAAAACTG TCTCCTATCC CAAGTCAGCA GGATGCAGTT 
CCTCACCGTG ATAAGCGGGG TTTGAAACAA TCCAGACTCC CACGAGAAGA AGTAAAGCTT 
GAAAGGGGGG TAACTCTGAA TGACACCTCA ACTCAGTGGT CCAAAAGCAT GAAACATTTG 
ACCCAGGGTA CCTTCACACA GATAGAACAC AACAAGATGG AGAAAAGGAC CATTACTCAG 
TCCTTCTTAC CAGATTGTTC TTTGAGGAGT CATAGGTCAA TTAGACCTAC AGATGTGACC 
AAGATCCCAC CTCAACATAA CTCTTCTCAT CCTCCAGCAT CAACCTATGG TTATAACTTG 
GGGGCAAGTC ATTCTGGGGT CCAAGAAACC AGTCATTTCT TACACGGAGC CAAGAAAAAT 
AATCTTTCTT TAGCCTTCCT AACATTGGAA GTGATCGGAA GTCAAAGAAA GAGCAGCACC 
TCGGGGAAAC AGGCCACAAA TCCACTCATG TACAAAAAAC TTGAGAACAT GTTTCTGTTG 
AAACCAGGCT TGTCTGAAGC ATCTGGGAAA GTTGTTCATC AGGGAGACTT GCCTACAAAA 
ACTAGCAAGG TTTCTCCTGC CCACCTGGAT CTCAGGGAAG AGATCTTCCT TCAGAAAACA 
CAGGGACTTG TTAAATTGAA TAAAGTCTAC AGGACTGGAA AAGTGCCATC TCTGAAATGG 
ACAACAGAAA GCTCTGAAGA GATTCCCTCC AAGCTGCTGG GTCTCCTTGC TTGGGATAAC 
CAGTCAGCTA CCTTGATACC AAAAGAAGAC TGGAAATCCC TAGAGAGGTC ACACAAACTC 
ACGTCTTTGA AGACAAAAGA TATCACTTTG CCCCTAGACC CTAGTGAAAA CTATTATTCA 
ATAGCAGTAA CAAATGAAGG AGAAGATAAG GCCCAAAGAG AAGCTCCCTG GCCAAAGCAA 
GGAGGGACTG GAAGGGTGTG CTGTCAAAAT CCACCAATCT TGAAACGCCA TCAAAGGGAG 
ATAACCCTTA CTACTTTTCA GCCAGAGCCA GACAAAACTG ACTATGATGA TACTTTGTCA 
ATTGAAACAA AGAGAGAAGA TTTTGACATT TATGGTGAAG ATGAAAATCA GGACCCCCGC 
AGCTTTCAAA AGAGAACACG CCACTATTTT ATTGCTGCAG TGGAGCGGCT CTGGGATTAT 
GGGATGAGTA GATCCCCCTA CGCACTAAGA AACAGGTCTC AGAATGGAGG AGTCCCTCAG 
TTCAAAAAGG TGGTATTCGA GGAATTTACT GATGGCTCCT TTACTCAGGC CATATACCGT 
GGACAACTAA ATGAACACCT GGGACTCTTG GGACCATATA TAAGAGCAGA AGTGGAAGAC 
AATATCATGG TAACTTTCAA AAACCAGGCC TCTCGTCCCT ACTCCTTCTA TTCTAGCCTT 
ATTTCTTATA ACGGAGATCA GAGACAAGGA GCAGAACCTC GAAAAAAGTT TGTCAAGCCT 
AATGAAACCC AAAGCTACTT TTGGAAAGTG CAGCACCATA TGGCACCCAC CAAAGATGAG 
TTTGACTGCA AAGCCTGGGC TTACTTTTCT GACGTTGATC TGGAAAAAGA TTTGCACTCA 
GGCTTGATTG GCCCCATTCT GATCTGCCGC GTGGACACGC TGAGTGCTGC TCATGGGAGA 
CAAGTAACAA TACAGGAATT CGCTCTGTTT TTCACCATTT TTGATGAAAC CAAGAGCTGG 
TACTTTGCCG AAAACATGGC AAGGAACTGC GGGGCGCCCT GCCATGTCCA GCCAGAGGAC 
CCTACTTTTC AAGAAAAGTA TCGCTTCCAT GCAATCAATG GCTACGTGAT GGATACACTC 
CCTGGCTTAG TCATGGCTCA GCATCAAAGG ATTAGGTGGT ATCTGCTCAG CATGGGCAGC 
AATGAAAATA TCCATTCCAT TCATTTCAGT GGCCATGTGT TCACTGTGCG AAAAACGGAG 
GAGTATAAAA TGGCGGTCTA CAATCTCTAC CCAGGTGTCT TTGAGACGGT GGAAATGCTA 
CCATCCAAGG TTGGGACTTG GCGGATAGAA TGTCTTATTG GCGAGCACCT ACAAGCTGGG 
ATGAGCACTC TCTTCCTGGT GTACAGCAAG GAGTGTCAAA TTCCACTGGG AATGGCTTCT 
GGACGCATTA GAGATTTTCA GATTACAGCT TCAGGACAAT ATGGACAGTG GGCCCCCAAG 
CTGGCCAGAC TTCATTATTC TGGATCAATC AACGCCTGGA GCACCAAGGA TCCCTCTCCT 
TGGATCAAGG TGGATCTGTT GGCGCCGATG ATTATTCACA GCATCCTGAC TCAGGGTGCC 
CGGCAGAAGT TCTCCAGCCT GTACATCTCT CAGTTTATCA TCATGTACAG CCTCGATGGA 
CAGCGGTGGC AGGGTTATCG GGGGAACTCC ACTGGGACCT TAATGGTGTT CTTTGGTAAT 
GTGGATTCAT CTGGAGTAAA ACACAATATT TTTAACCCTC CAATTATTGC TAGATATATC 
CGTTTACACC CAACTCATTA CAGCATCCGC AGCACTCTTC GCATGGAGTT GATGGGCTGT 
GATTTAAACA GTTGCAACAT GCCACTGGGA ATGGAGAATA AAGCTATATC AGATACACAG 
ATTACTGCCT CATCCCACTT ACGCAACATG TTTGCCACCT GGTCTCCTTC ACAAGCCCGA 
CTTAACCTTC AAGGGAGGAC AAATGCCTGG AGGCCCCAGG TGAATAATCC AAAAGAGTGG 
CTACAAGTGG ACTTCCAGAA GACAATGAGA GTTACAGGAA TAACTACCCA AGGGGTGAAA 
TCTCTGCTTA CCAGCATGTA TGTGAAGGAG TTCCTCATAT CCAGTAGTCA AGATGGCCAT 
AACTGGACTC CATTTCTTCA GAATGGCAAA GTGAAGGTTT TTCAGGGAAA TCAAGACTCC 
TTCACCCCCG TGGTGAATGC TCTAGACCCC CCGCTGTTTA CCCGCTTCCT TCGGATTCAC 
CCGCGGAGCT GGGCGCACCA TATCGCCCTG AGGCTGGAGC TTTTGGGCTG CGAGGCACAG 
CAGCAGTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138980.1
CDS	182..7153
Translation	MHIKLSTCFFLCLWPCSFSAIRRYYLGAVELSWDYMQSELLSELHVNTRFPPRVPKPFPFNTSVMYRKTVFVEFTDQLFNIAKPRPPWMGLLGPTIQAEVYDTVVITFKNMASHPVSLHAIGVSYWKSSEGAAYEDETSQREKEDDKVIPGKSHTYVWQILKENGPTASDPPCLTYSYLSHVDLVKDVNSGLIGALLICREGTLIKERTQTLHKFVLLFAVFDEGKSWHSGKNESLTRVMDSASALHTINGYINRSLPGLIGCHKKSVYWHVIGMGTTPEVHSIFLEGHTFLVRNHRQASLEISPITFLTAQTVLMDCGQFLLFCRISSHQHDGMEAYVKVDSCPEEPRLWMKNNQEEDYDDGLDDSDMDVVRFDGDSAPPFIQMRSVAKKHPKTWVHYIAAEEDDWDYAPSVLTTSDRSYKSLYLNHGPQRIGRKYRKVRFIAYTDGTFKTREAIQHESGILGPLLYGEVGDTLLIIFKNQASRPYNIYPHGITDVSPLHSGRFPKGVKHLKDMPILPGEVFKYKWTVTVEDGPTKSDPRCLTRYYSSFINLEKDLASGLIGPLLICYKESVDQRGNQMMSDKRNVILFSVFDENKSWYLTENIQRFLPNGVQPQDPEFQVSNVMHSINGYVFDSLQLSVCLHEVAYWYILSVGAQIDFLSVFFSGYTFKHKMVYEDTLTLFPFSGETVFMSMENPGLWVLGCHNSDFRNRGMTALLKVDSCDRNVGDYYDTYEDIPTLLSDDNVIEPRSFSKNSRYPSTRQKQFKPTMTPENDMEKIDPQSGEKMQLLTEQSVSSSDLLMLLGQNPTPHRLSLSDLQETRNEATDHLPGPTERIKGPSPSEVAHLRPELHHSGERVFTQEPELPVRLNENLGTTIMAELNKLNLKISSSSNNLMPLPTIPSDKLSAGTKKTGSFGPPNMPVNFNSQLGAIVFGKNLSHVIGSRVPLGLSKEDNNSKLLEAALMNSQESSLGENALSLESDRLFKEERVHGPVSLNKDDALFKVNISLVKTDKAPANSTTNRKTHIDDPTVLIENSTSVWQDIVIDSNTNFQEVTSLIHDELFMHKNTTSFGLSHMSNKTTSSKKREMIHQEKEGPKPLDTKYPDTPFFKTLFLPDSTNVIKRTHDKNSLSSGQRPSPKQLTSSVSENSVKDQNVLSEKNKAVAGEDEFSNNTSLKEMIFPNTKSIVLTNLANVQENDTDNQGKKSQEETEKKETLIPENTVLPQVYTVIGTKNFLKNLFSLSTKQNVRGLDEETYTPGLQDNRSLSDSAKRARIHAAHFSKGRVEANVEGLGNQTKQMVEKSPSTTKLSPIPSQQDAVPHRDKRGLKQSRLPREEVKLERGVTLNDTSTQWSKSMKHLTQGTFTQIEHNKMEKRTITQSFLPDCSLRSHRSIRPTDVTKIPPQHNSSHPPASTYGYNLGASHSGVQETSHFLHGAKKNNLSLAFLTLEVIGSQRKSSTSGKQATNPLMYKKLENMFLLKPGLSEASGKVVHQGDLPTKTSKVSPAHLDLREEIFLQKTQGLVKLNKVYRTGKVPSLKWTTESSEEIPSKLLGLLAWDNQSATLIPKEDWKSLERSHKLTSLKTKDITLPLDPSENYYSIAVTNEGEDKAQREAPWPKQGGTGRVCCQNPPILKRHQREITLTTFQPEPDKTDYDDTLSIETKREDFDIYGEDENQDPRSFQKRTRHYFIAAVERLWDYGMSRSPYALRNRSQNGGVPQFKKVVFEEFTDGSFTQAIYRGQLNEHLGLLGPYIRAEVEDNIMVTFKNQASRPYSFYSSLISYNGDQRQGAEPRKKFVKPNETQSYFWKVQHHMAPTKDEFDCKAWAYFSDVDLEKDLHSGLIGPILICRVDTLSAAHGRQVTIQEFALFFTIFDETKSWYFAENMARNCGAPCHVQPEDPTFQEKYRFHAINGYVMDTLPGLVMAQHQRIRWYLLSMGSNENIHSIHFSGHVFTVRKTEEYKMAVYNLYPGVFETVEMLPSKVGTWRIECLIGEHLQAGMSTLFLVYSKECQIPLGMASGRIRDFQITASGQYGQWAPKLARLHYSGSINAWSTKDPSPWIKVDLLAPMIIHSILTQGARQKFSSLYISQFIIMYSLDGQRWQGYRGNSTGTLMVFFGNVDSSGVKHNIFNPPIIARYIRLHPTHYSIRSTLRMELMGCDLNSCNMPLGMENKAISDTQITASSHLRNMFATWSPSQARLNLQGRTNAWRPQVNNPKEWLQVDFQKTMRVTGITTQGVKSLLTSMYVKEFLISSSQDGHNWTPFLQNGKVKVFQGNQDSFTPVVNALDPPLFTRFLRIHPRSWAHHIALRLELLGCEAQQQY

Target ORF information:

RefSeq Version	NM_001145508.1
Organism	Bos taurus(cattle)
Definition	Bos taurus coagulation factor VIII (F8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001145508.1

ORF Insert Sequence:

ATGCACATCA AGCTCTCTAC CTGCTTCTTT CTGTGCCTCT GGCCATGCAG CTTCAGTGCC 
ATCAGAAGAT ACTACCTGGG TGCAGTGGAA CTCTCCTGGG ACTATATGCA AAGTGAACTG 
CTCAGTGAGC TGCATGTGAA CACAAGGTTT CCTCCCAGAG TGCCCAAACC TTTTCCATTC 
AACACCTCAG TCATGTACAG AAAGACTGTG TTTGTAGAGT TCACGGATCA ACTTTTCAAC 
ATCGCCAAGC CCAGGCCACC ATGGATGGGT CTGCTGGGTC CAACTATCCA GGCTGAGGTT 
TATGACACCG TGGTCATTAC ATTTAAGAAT ATGGCTTCTC ATCCTGTTAG TCTTCATGCT 
ATTGGCGTAT CCTACTGGAA ATCTTCTGAA GGTGCTGCAT ATGAGGATGA AACCAGCCAA 
AGGGAGAAGG AAGATGACAA AGTCATTCCT GGTAAAAGTC ATACCTACGT CTGGCAGATC 
CTGAAAGAAA ATGGTCCAAC AGCCTCTGAC CCACCATGTC TCACCTACTC ATATCTTTCT 
CATGTGGACC TGGTGAAAGA CGTGAACTCA GGTCTCATTG GAGCCCTGCT AATTTGTAGG 
GAAGGAACTC TGATCAAAGA AAGGACACAG ACCTTGCACA AATTCGTACT ACTGTTTGCT 
GTATTTGATG AAGGGAAAAG TTGGCACTCG GGAAAAAATG AGTCCTTGAC ACGTGTTATG 
GATTCTGCAT CTGCACTGCA CACCATCAAT GGCTATATAA ACAGGTCTCT GCCAGGTCTG 
ATTGGATGTC ATAAGAAATC AGTCTATTGG CACGTGATTG GAATGGGCAC CACCCCGGAA 
GTGCACTCAA TTTTCCTTGA AGGCCACACA TTTCTCGTGA GGAACCATCG CCAGGCTTCC 
TTGGAGATCT CACCAATAAC TTTCCTTACC GCTCAGACAG TCCTGATGGA CTGTGGCCAG 
TTTCTACTGT TTTGTCGTAT CTCTTCCCAC CAACATGATG GTATGGAAGC TTATGTCAAA 
GTAGATAGTT GCCCAGAGGA ACCCCGACTA TGGATGAAAA ATAACCAAGA AGAAGATTAT 
GATGATGGTT TGGATGACTC TGACATGGAT GTGGTCAGGT TTGATGGCGA CAGTGCGCCT 
CCCTTTATCC AAATGCGCTC AGTTGCAAAG AAGCATCCTA AAACCTGGGT CCACTACATT 
GCTGCTGAAG AGGATGACTG GGACTATGCC CCCTCGGTCC TCACCACCAG TGACAGAAGT 
TATAAAAGTC TGTATCTGAA CCACGGTCCT CAGCGGATTG GTAGGAAGTA CAGAAAAGTA 
CGGTTTATAG CTTACACAGA TGGAACATTT AAGACTCGTG AAGCTATTCA GCATGAATCA 
GGGATCCTGG GGCCTTTACT TTATGGAGAA GTTGGAGACA CACTTTTGAT TATATTTAAG 
AATCAAGCAA GCCGGCCATA TAACATCTAC CCTCATGGAA TCACTGATGT CAGTCCTTTG 
CACTCAGGGA GATTTCCAAA AGGTGTGAAA CATTTGAAAG ACATGCCAAT TCTGCCAGGA 
GAAGTGTTCA AGTATAAATG GACAGTGACT GTAGAAGATG GGCCAACTAA ATCAGATCCT 
CGGTGTCTGA CCCGATATTA CTCGAGTTTC ATTAACTTAG AGAAAGATCT AGCTTCAGGA 
CTCATTGGCC CCCTCCTCAT CTGCTACAAA GAATCTGTAG ATCAAAGAGG AAACCAGATG 
ATGTCAGACA AGAGAAATGT CATCCTGTTT TCTGTATTTG ATGAGAATAA AAGTTGGTAC 
CTCACAGAGA ATATTCAGCG CTTCCTCCCC AATGGAGTAC AGCCCCAGGA TCCAGAGTTC 
CAAGTCTCCA ATGTCATGCA TAGCATCAAT GGCTATGTTT TTGATAGCTT GCAGCTGTCG 
GTTTGTTTGC ATGAGGTGGC ATACTGGTAC ATTCTAAGTG TTGGAGCCCA AATTGACTTC 
CTCTCTGTCT TCTTCTCTGG ATATACCTTC AAACACAAAA TGGTCTATGA AGATACACTC 
ACCCTATTCC CCTTTTCAGG AGAAACTGTC TTCATGTCAA TGGAAAATCC AGGTCTGTGG 
GTTCTGGGGT GCCACAACTC AGACTTTCGA AACAGAGGCA TGACAGCCTT ACTGAAGGTT 
GATAGTTGTG ACAGGAACGT TGGCGATTAT TATGACACAT ATGAAGATAT TCCAACCTTG 
CTGAGTGACG ACAATGTCAT TGAACCCAGA AGCTTCTCCA AGAATTCAAG GTACCCTAGC 
ACTAGGCAAA AGCAATTCAA ACCCACCATG ACTCCAGAAA ATGACATGGA GAAGATTGAC 
CCTCAGTCTG GAGAGAAAAT GCAGCTGCTT ACAGAACAAA GTGTCTCCTC TAGTGATCTG 
TTGATGCTCT TGGGACAGAA TCCTACTCCA CACAGATTAT CCTTATCTGA TCTCCAAGAA 
ACCAGAAATG AGGCTACTGA TCATTTACCT GGACCAACAG AAAGAATCAA GGGCCCATCC 
CCATCTGAAG TGGCACATCT TAGACCAGAG CTGCATCACA GTGGGGAAAG AGTATTTACT 
CAAGAGCCAG AACTGCCAGT AAGATTAAAT GAGAATTTGG GAACAACTAT AATGGCAGAG 
TTGAACAAAC TTAATTTAAA AATTTCAAGT TCATCAAACA ATCTAATGCC TTTACCAACA 
ATTCCATCAG ACAAGTTGTC AGCAGGTACT AAAAAGACAG GTTCCTTCGG ACCCCCAAAT 
ATGCCAGTTA ATTTTAATAG TCAATTAGGT GCCATTGTAT TTGGCAAAAA TTTATCTCAT 
GTTATTGGAT CACGTGTACC TTTGGGCTTG AGTAAAGAAG ATAATAATTC CAAGTTGTTA 
GAAGCAGCTT TAATGAATAG TCAAGAAAGT TCACTGGGAG AAAATGCATT ATCACTGGAG 
AGTGATAGGC TATTTAAAGA GGAAAGAGTT CATGGACCTG TGTCATTGAA CAAAGATGAT 
GCTTTATTTA AAGTGAATAT CTCTTTGGTA AAGACAGACA AGGCACCAGC TAACTCAACA 
ACTAATAGAA AGACTCACAT TGATGACCCA ACAGTATTAA TTGAGAATAG TACATCAGTC 
TGGCAGGATA TTGTAATAGA CAGTAATACC AACTTTCAAG AAGTGACTTC TTTGATTCAT 
GATGAATTGT TTATGCACAA AAATACTACA TCTTTTGGAC TAAGCCATAT GTCAAATAAA 
ACTACTTCAT CCAAAAAAAG GGAAATGATC CACCAAGAAA AAGAAGGCCC TAAGCCACTA 
GATACAAAAT ATCCAGATAC ACCATTCTTC AAGACTCTGT TTTTGCCAGA TTCAACAAAC 
GTGATAAAAA GGACCCACGA CAAGAACTCC CTAAGCTCTG GGCAAAGGCC CAGTCCAAAG 
CAATTAACAT CATCAGTATC AGAAAACTCT GTGAAAGATC AGAATGTCTT ATCAGAAAAG 
AATAAGGCAG TAGCAGGAGA GGATGAATTT AGCAACAACA CCAGCCTCAA AGAGATGATT 
TTTCCAAATA CCAAAAGCAT AGTTCTTACT AACTTGGCTA ATGTCCAAGA AAATGATACA 
GACAATCAAG GAAAAAAATC CCAGGAAGAA ACAGAAAAGA AAGAGACATT AATCCCTGAG 
AACACAGTTT TGCCTCAGGT GTATACAGTG ATTGGAACTA AGAATTTCCT GAAGAATCTC 
TTTTCACTGA GCACTAAGCA AAATGTCAGA GGTTTAGATG AAGAGACATA TACTCCAGGA 
CTTCAAGACA ACAGGTCATT AAGTGATTCG GCAAAAAGAG CCAGGATTCA TGCAGCCCAT 
TTCTCAAAAG GAAGGGTGGA AGCAAACGTG GAAGGCTTAG GGAATCAAAC AAAACAAATG 
GTAGAGAAAT CCCCAAGCAC TACAAAACTG TCTCCTATCC CAAGTCAGCA GGATGCAGTT 
CCTCACCGTG ATAAGCGGGG TTTGAAACAA TCCAGACTCC CACGAGAAGA AGTAAAGCTT 
GAAAGGGGGG TAACTCTGAA TGACACCTCA ACTCAGTGGT CCAAAAGCAT GAAACATTTG 
ACCCAGGGTA CCTTCACACA GATAGAACAC AACAAGATGG AGAAAAGGAC CATTACTCAG 
TCCTTCTTAC CAGATTGTTC TTTGAGGAGT CATAGGTCAA TTAGACCTAC AGATGTGACC 
AAGATCCCAC CTCAACATAA CTCTTCTCAT CCTCCAGCAT CAACCTATGG TTATAACTTG 
GGGGCAAGTC ATTCTGGGGT CCAAGAAACC AGTCATTTCT TACACGGAGC CAAGAAAAAT 
AATCTTTCTT TAGCCTTCCT AACATTGGAA GTGATCGGAA GTCAAAGAAA GAGCAGCACC 
TCGGGGAAAC AGGCCACAAA TCCACTCATG TACAAAAAAC TTGAGAACAT GTTTCTGTTG 
AAACCAGGCT TGTCTGAAGC ATCTGGGAAA GTTGTTCATC AGGGAGACTT GCCTACAAAA 
ACTAGCAAGG TTTCTCCTGC CCACCTGGAT CTCAGGGAAG AGATCTTCCT TCAGAAAACA 
CAGGGACTTG TTAAATTGAA TAAAGTCTAC AGGACTGGAA AAGTGCCATC TCTGAAATGG 
ACAACAGAAA GCTCTGAAGA GATTCCCTCC AAGCTGCTGG GTCTCCTTGC TTGGGATAAC 
CAGTCAGCTA CCTTGATACC AAAAGAAGAC TGGAAATCCC TAGAGAGGTC ACACAAACTC 
ACGTCTTTGA AGACAAAAGA TATCACTTTG CCCCTAGACC CTAGTGAAAA CTATTATTCA 
ATAGCAGTAA CAAATGAAGG AGAAGATAAG GCCCAAAGAG AAGCTCCCTG GCCAAAGCAA 
GGAGGGACTG GAAGGGTGTG CTGTCAAAAT CCACCAATCT TGAAACGCCA TCAAAGGGAG 
ATAACCCTTA CTACTTTTCA GCCAGAGCCA GACAAAACTG ACTATGATGA TACTTTGTCA 
ATTGAAACAA AGAGAGAAGA TTTTGACATT TATGGTGAAG ATGAAAATCA GGACCCCCGC 
AGCTTTCAAA AGAGAACACG CCACTATTTT ATTGCTGCAG TGGAGCGGCT CTGGGATTAT 
GGGATGAGTA GATCCCCCTA CGCACTAAGA AACAGGTCTC AGAATGGAGG AGTCCCTCAG 
TTCAAAAAGG TGGTATTCGA GGAATTTACT GATGGCTCCT TTACTCAGGC CATATACCGT 
GGACAACTAA ATGAACACCT GGGACTCTTG GGACCATATA TAAGAGCAGA AGTGGAAGAC 
AATATCATGG TAACTTTCAA AAACCAGGCC TCTCGTCCCT ACTCCTTCTA TTCTAGCCTT 
ATTTCTTATA ACGGAGATCA GAGACAAGGA GCAGAACCTC GAAAAAAGTT TGTCAAGCCT 
AATGAAACCC AAAGCTACTT TTGGAAAGTG CAGCACCATA TGGCACCCAC CAAAGATGAG 
TTTGACTGCA AAGCCTGGGC TTACTTTTCT GACGTTGATC TGGAAAAAGA TTTGCACTCA 
GGCTTGATTG GCCCCATTCT GATCTGCCGC GTGGACACGC TGAGTGCTGC TCATGGGAGA 
CAAGTAACAA TACAGGAATT CGCTCTGTTT TTCACCATTT TTGATGAAAC CAAGAGCTGG 
TACTTTGCCG AAAACATGGC AAGGAACTGC GGGGCGCCCT GCCATGTCCA GCCAGAGGAC 
CCTACTTTTC AAGAAAAGTA TCGCTTCCAT GCAATCAATG GCTACGTGAT GGATACACTC 
CCTGGCTTAG TCATGGCTCA GCATCAAAGG ATTAGGTGGT ATCTGCTCAG CATGGGCAGC 
AATGAAAATA TCCATTCCAT TCATTTCAGT GGCCATGTGT TCACTGTGCG AAAAACGGAG 
GAGTATAAAA TGGCGGTCTA CAATCTCTAC CCAGGTGTCT TTGAGACGGT GGAAATGCTA 
CCATCCAAGG TTGGGACTTG GCGGATAGAA TGTCTTATTG GCGAGCACCT ACAAGCTGGG 
ATGAGCACTC TCTTCCTGGT GTACAGCAAG GAGTGTCAAA TTCCACTGGG AATGGCTTCT 
GGACGCATTA GAGATTTTCA GATTACAGCT TCAGGACAAT ATGGACAGTG GGCCCCCAAG 
CTGGCCAGAC TTCATTATTC TGGATCAATC AACGCCTGGA GCACCAAGGA TCCCTCTCCT 
TGGATCAAGG TGGATCTGTT GGCGCCGATG ATTATTCACA GCATCCTGAC TCAGGGTGCC 
CGGCAGAAGT TCTCCAGCCT GTACATCTCT CAGTTTATCA TCATGTACAG CCTCGATGGA 
CAGCGGTGGC AGGGTTATCG GGGGAACTCC ACTGGGACCT TAATGGTGTT CTTTGGTAAT 
GTGGATTCAT CTGGAGTAAA ACACAATATT TTTAACCCTC CAATTATTGC TAGATATATC 
CGTTTACACC CAACTCATTA CAGCATCCGC AGCACTCTTC GCATGGAGTT GATGGGCTGT 
GATTTAAACA GTTGCAACAT GCCACTGGGA ATGGAGAATA AAGCTATATC AGATACACAG 
ATTACTGCCT CATCCCACTT ACGCAACATG TTTGCCACCT GGTCTCCTTC ACAAGCCCGA 
CTTAACCTTC AAGGGAGGAC AAATGCCTGG AGGCCCCAGG TGAATAATCC AAAAGAGTGG 
CTACAAGTGG ACTTCCAGAA GACAATGAGA GTTACAGGAA TAACTACCCA AGGGGTGAAA 
TCTCTGCTTA CCAGCATGTA TGTGAAGGAG TTCCTCATAT CCAGTAGTCA AGATGGCCAT 
AACTGGACTC CATTTCTTCA GAATGGCAAA GTGAAGGTTT TTCAGGGAAA TCAAGACTCC 
TTCACCCCCG TGGTGAATGC TCTAGACCCC CCGCTGTTTA CCCGCTTCCT TCGGATTCAC 
CCGCGGAGCT GGGCGCACCA TATCGCCCTG AGGCTGGAGC TTTTGGGCTG CGAGGCACAG 
CAGCAGTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

F8 ( XM_024987718.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024843486.1 Bos taurus coagulation factor VIII (F8), transcript variant X3, mRNA.

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CloneID	OBa238231
Clone ID Related Accession (Same CDS sequence)	XM_024987718.1
Accession Version	XM_024987718.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	coagulation factor VIII isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACCCG CGCGGCTTCG CCGCGGGAGG ACGCGGAGTG GCGCGCCGCC GCCGTCTGCG 
CGAGGTGGCT TCCGGTTTCC TCCCAGAGTG CCCAAACCTT TTCCATTCAA CACCTCAGTC 
ATGTACAGAA AGACTGTGTT TGTAGAGTTC ACGGATCAAC TTTTCAACAT CGCCAAGCCC 
AGGCCACCAT GGATGGGTCT GCTGGGTCCA ACTATCCAGG CTGAGGTTTA TGACACCGTG 
GTCATTACAT TTAAGAATAT GGCTTCTCAT CCTGTTAGTC TTCATGCTAT TGGCGTATCC 
TACTGGAAAT CTTCTGAAGG TGCTGCATAT GAGGATGAAA CCAGCCAAAG GGAGAAGGAA 
GATGACAAAG TCATTCCTGG TAAAAGTCAT ACCTACGTCT GGCAGATCCT GAAAGAAAAT 
GGTCCAACAG CCTCTGACCC ACCATGTCTC ACCTACTCAT ATCTTTCTCA TGTGGACCTG 
GTGAAAGACG TGAACTCAGG TCTCATTGGA GCCCTGCTAA TTTGTAGGGA AGGAACTCTG 
ATCAAAGAAA GGACACAGAC CTTGCACAAA TTCGTACTAC TGTTTGCTGT ATTTGATGAA 
GGGAAAAGTT GGCACTCGGG AAAAAATGAG TCCTTGACAC GTGTTATGGA TTCTGCATCT 
GCACTGCACA CCATCAATGG CTATATAAAC AGGTCTCTGC CAGGTCTGAT TGGATGTCAT 
AAGAAATCAG TCTATTGGCA CGTGATTGGA ATGGGCACCA CCCCGGAAGT GCACTCAATT 
TTCCTTGAAG GCCACACATT TCTCGTGAGG AACCATCGCC AGGCTTCCTT GGAGATCTCA 
CCAATAACTT TCCTTACCGC TCAGACAGTC CTGATGGACT GTGGCCAGTT TCTACTGTTT 
TGTCGTATCT CTTCCCACCA ACATGATGGT ATGGAAGCTT ATGTCAAAGT AGATAGTTGC 
CCAGAGGAAC CCCGACTATG GATGAAAAAT AACCAAGAAG AAGATTATGA TGATGGTTTG 
GATGACTCTG ACATGGATGT GGTCAGGTTT GATGGCGACA GTGCGCCTCC CTTTATCCAA 
ATGCGCTCAG TTGCAAAGAA GCATCCTAAA ACCTGGGTCC ACTACATTGC TGCTGAAGAG 
GATGACTGGG ACTATGCCCC CTCGGTCCTC ACCACCAGTG ACAGAAGTTA TAAAAGTCTG 
TATCTGAACC ACGGTCCTCA GCGGATTGGT AGGAAGTACA GAAAAGTACG GTTTATAGCT 
TACACAGATG GAACATTTAA GACTCGTGAA GCTATTCAGC ATGAATCAGG GATCCTGGGG 
CCTTTACTTT ATGGAGAAGT TGGAGACACA CTTTTGATTA TATTTAAGAA TCAAGCAAGC 
CGGCCATATA ACATCTACCC TCATGGAATC ACTGATGTCA GTCCTTTGCA CTCAGGGAGA 
TTTCCAAAAG GTGTGAAACA TTTGAAAGAC ATGCCAATTC TGCCAGGAGA AGTGTTCAAG 
TATAAATGGA CAGTGACTGT AGAAGATGGG CCAACTAAAT CAGATCCTCG GTGTCTGACC 
CGATATTACT CGAGTTTCAT TAACTTAGAG AAAGATCTAG CTTCAGGACT CATTGGCCCC 
CTCCTCATCT GCTACAAAGA ATCTGTAGAT CAAAGAGGAA ACCAGATGAT GTCAGACAAG 
AGAAATGTCA TCCTGTTTTC TGTATTTGAT GAGAATAAAA GTTGGTACCT CACAGAGAAT 
ATTCAGCGCT TCCTCCCCAA TGGAGTACAG CCCCAGGATC CAGAGTTCCA AGTCTCCAAT 
GTCATGCATA GCATCAATGG CTATGTTTTT GATAGCTTGC AGCTGTCGGT TTGTTTGCAT 
GAGGTGGCAT ACTGGTACAT TCTAAGTGTT GGAGCCCAAA TTGACTTCCT CTCTGTCTTC 
TTCTCTGGAT ATACCTTCAA ACACAAAATG GTCTATGAAG ATACACTCAC CCTATTCCCC 
TTTTCAGGAG AAACTGTCTT CATGTCAATG GAAAATCCAG GTCTGTGGGT TCTGGGGTGC 
CACAACTCAG ACTTTCGAAA CAGAGGCATG ACAGCCTTAC TGAAGGTTGA TAGTTGTGAC 
AGGAACGTTG GCGATTATTA TGACACATAT GAAGATATTC CAACCTTGCT GAGTGACGAC 
AATGTCATTG AACCCAGAAG CTTCTCCAAG AATTCAAGGT ACCCTAGCAC TAGGCAAAAG 
CAATTCAAAC CCACCATGAC TCCAGAAAAT GACATGGAGA AGATTGACCC TCAGTCTGGA 
GAGAAAATGC AGCTGCTTAC AGAACAAAGT GTCTCCTCTA GTGATCTGTT GATGCTCTTG 
GGACAGAATC CTACTCCACA CAGATTATCC TTATCTGATC TCCAAGAAAC CAGAAATGAG 
GCTACTGATC ATTTACCTGG ACCAACAGAA AGAATCAAGG GCCCATCCCC ATCTGAAGTG 
GCACATCTTA GACCAGAGCT GCATCACAGT GGGGAAAGAG TATTTACTCA AGAGCCAGAA 
CTGCCAGTAA GATTAAATGA GAATTTGGGA ACAACTATAA TGGCAGAGTT GAACAAACTT 
AATTTAAAAA TTTCAAGTTC ATCAAACAAT CTAATGCCTT TACCAACAAT TCCATCAGAC 
AAGTTGTCAG CAGGTACTAA AAAGACAGGT TCCTTCGGAC CCCCAAATAT GCCAGTTAAT 
TTTAATAGTC AATTAGGTGC CATTGTATTT GGCAAAAATT TATCTCATGT TATTGGATCA 
CGTGTACCTT TGGGCTTGAG TAAAGAAGAT AATAATTCCA AGTTGTTAGA AGCAGCTTTA 
ATGAATAGTC AAGAAAGTTC ACTGGGAGAA AATGCATTAT CACTGGAGAG TGATAGGCTA 
TTTAAAGAGG AAAGAGTTCA TGGACCTGTG TCATTGAACA AAGATGATGC TTTATTTAAA 
GTGAATATCT CTTTGGTAAA GACAGACAAG GCACCAGCTA ACTCAACAAC TAATAGAAAG 
ACTCACATTG ATGACCCAAC AGTATTAATT GAGAATAGTA CATCAGTCTG GCAGGATATT 
GTAATAGACA GTAATACCAA CTTTCAAGAA GTGACTTCTT TGATTCATGA TGAATTGTTT 
ATGCACAAAA ATACTACATC TTTTGGACTA AGCCATATGT CAAATAAAAC TACTTCATCC 
AAAAAAAGGG AAATGATCCA CCAAGAAAAA GAAGGCCCTA AGCCACTAGA TACAAAATAT 
CCAGATACAC CATTCTTCAA GACTCTGTTT TTGCCAGATT CAACAAACGT GATAAAAAGG 
ACCCACGACA AGAACTCCCT AAGCTCTGGG CAAAGGCCCA GTCCAAAGCA ATTAACATCA 
TCAGTATCAG AAAACTCTGT GAAAGATCAG AATGTCTTAT CAGAAAAGAA TAAGGCAGTA 
GCAGGAGAGG ATGAATTTAG CAACAACACC AGCCTCAAAG AGATGATTTT TCCAAATACC 
AAAAGCATAG TTCTTACTAA CTTGGCTAAT GTCCAAGAAA ATGATACAGA CAATCAAGGA 
AAAAAATCCC AGGAAGAAAC AGAAAAGAAA GAGACATTAA TCCCTGAGAA CACAGTTTTG 
CCTCAGGTGT ATACAGTGAT TGGAACTAAG AATTTCCTGA AGAATCTCTT TTCACTGAGC 
ACTAAGCAAA ATGTCAGAGG TTTAGATGAA GAGACATATA CTCCAGGACT TCAAGACAAC 
AGGTCATTAA GTGATTCGGC AAAAAGAGCC AGGATTCATG CAGCCCATTT CTCAAAAGGA 
AGGGTGGAAG CAAACGTGGA AGGCTTAGGG AATCAAACAA AACAAATGGT AGAGAAATCT 
CCAAGCACTA CAAAACTGTC TCCTATCCCA AGTCAGCAGG ATGCAGTTCC TCACCGTGAT 
AAGCGGGGTT TGAAACAATC CAGACTCCCA CGAGAAGAAG TAAAGCTTGA AAGGGGGGTA 
ACTCTGAATG ACACCTCAAC TCAGTGGTCC AAAAGCATGA AACATTTGAC CCAGGGTACC 
TTCACACAGA TAGAACACAA CAAGATGGAG AAAAGGACCA TTACTCAGTC CTTCTTACCA 
GATTGTTCTT TGAGGAGTCA TAGGTCAATT AGACCTACAG ATGTGACCAA GATCCCACCT 
CAACATAACT CTTCTCATCC TCCAGCATCA ACCTATGGTT ATAACTTGGG GGCAAGTCAT 
TCTGGGGTCC AAGAAACCAG TCATTTCTTA CACGGAGCCA AGAAAAATAA TCTTTCTTTA 
GCCTTCCTAA CATTGGAAGT GATCGGAAGT CAAAGAAAGA GCAGCACCCC GGGGAAACAG 
GCCACAAATC CACTCATGTA CAAAAAACTT GAGAACATGT TTCTGTTGAA ACCAGGCTTG 
TCTGAAGCAT CTGGGAAAGT TGTTCATCAG GAAGACTTGC CTACAAAAAC TAGCAAGGTT 
TCTCCTGCCC ACCTGGATCT CAGGGAAGAG ATCTTCCTTC AGAAAACACA GGGACTTGTT 
AAATTGAATA AAGTCTACAG GACTGGAAAA GTGCCATCTC TGAAATGGAC AACAGAAAGC 
TCTGAAGAGA TTCCCTCCAA GCTGCTGGGT CTCCTTGCTT GGGATAACCA GTCAGCTACC 
TTGATACCAA AAGAAGACTG GAAATCCCTA GAGAGGTCAC ACAAACTCAC GTCTTTGAAG 
ACAAAAGATA TCACTTTGCC CCTAGACCCT AGTGAAAACT ATTATTCAAT AGCAGTAACA 
AATGAAGGAG AAGATAAGGC CCAAAGAGAA GCTCCCTGGC CAAAGCAAGG AGGGACTGGA 
AGGGTGTGCT GTCAAAATCC ACCAATCTTG AAACGCCATC AAAGGGAGAT AACCCTTACT 
ACTTTTCAGC CAGAGCCAGA CAAAACTGAC TATGATGATA CTTTGTCAAT TGAAACAAAG 
AGAGAAGATT TTGACATTTA TGGTGAAGAT GAAAATCAGG ACCCCCGCAG CTTTCAAAAG 
AGAACACGCC ACTATTTTAT TGCTGCAGTG GAGCGGCTCT GGGATTATGG GATGAGTAGA 
TCCCCCTACG CACTAAGAAA CAGGTCTCAG AATGGAGGAG TCCCTCAGTT CAAAAAGGTG 
GTATTCGAGG AATTTACTGA TGGCTCCTTT ACTCAGGCCA TATACCGTGG ACAACTAAAT 
GAACACCTGG GACTCTTGGG ACCATATATA AGAGCAGAAG TGGAAGACAA TATCATGGTA 
ACTTTCAAAA ACCAGGCCTC TCGTCCCTAC TCCTTCTATT CTAGCCTTAT TTCTTATAAC 
GGAGATCAGA GACAAGGAGC AGAACCTCGA AAAAAGTTTG TCAAGCCTAA TGAAACCCAA 
AGCTACTTTT GGAAAGTGCA GCACCATATG GCACCCACCA AAGATGAGTT TGACTGCAAA 
GCCTGGGCTT ACTTTTCTGA CGTTGATCTG GAAAAAGATT TGCACTCAGG CTTGATTGGC 
CCCATTCTGA TCTGCCGCGT GGACACGCTG AGTGCTGCTC ATGGGAGACA AGTAACAATA 
CAGGAATTCG CTCTGTTTTT CACCATTTTT GATGAAACCA AGAGCTGGTA CTTTGCCGAA 
AACATGGCAA GGAACTGCGG GGCGCCCTGC CATGTCCAGC CAGAGGACCC TACTTTTCAA 
GAAAAGTATC GCTTCCATGC AATCAATGGC TACGTGATGG ATACACTCCC TGGCTTAGTC 
ATGGCTCAGC ATCAAAGGAT TAGGTGGTAT CTGCTCAGCA TGGGCAGCAA TGAAAATATC 
CATTCCATTC ATTTCAGTGG CCATGTGTTC ACTGTGCGAA AAACGGAGGA GTATAAAATG 
GCGGTCTACA ATCTCTACCC AGGTGTCTTT GAGACGGTGG AAATGCTACC ATCCAAGGTT 
GGGACTTGGC GGATAGAATG TCTTATTGGC GAGCACCTAC AAGCTGGGAT GAGCACTCTC 
TTCCTGGTGT ACAGCAAGGA GTGTCAAATT CCACTGGGAA TGGCTTCTGG ACGCATTAGA 
GATTTTCAGA TTACAGCTTC AGGACAATAT GGACAGTGGG CCCCCAAGCT GGCCAGACTT 
CATTATTCTG GATCAATCAA CGCCTGGAGC ACCAAGGATC CCTCTCCTTG GATCAAGGTG 
GATCTGTTGG CGCCGATGAT TATTCACAGC ATCCTGACTC AGGGTGCCCG GCAGAAGTTC 
TCCAGCCTGT ACATCTCTCA GTTTATCATC ATGTACAGCC TCGATGGACA GCGGTGGCAG 
GGTTATCGGG GGAACTCCAC TGGGACCTTA ATGGTGTTCT TTGGTAATGT GGATTCATCT 
GGAGTAAAAC ACAATATTTT TAACCCTCCA ATTATTGCTA GATATATCCG TTTACACCCA 
ACTCATTACA GCATCCGCAG CACTCTTCGC ATGGAGTTGA TGGGCTGTGA TTTAAACAGT 
TGCAACATGC CACTGGGAAT GGAGAATAAA GCTATATCAG ATACACAGAT TACTGCCTCA 
TCCCACTTAC GCAACATGTT TGCCACCTGG TCTCCTTCAC AAGCCCGACT TAACCTTCAA 
GGGAGGACAA ATGCCTGGAG GCCCCAGGTG AATAATCCAA AAGAGTGGCT ACAAGTGGAC 
TTCCAGAAGA CAATGAGAGT TACAGGAATA ACTACCCAAG GGGTGAAATC TCTGCTTACC 
AGCATGTATG TGAAGGAGTT CCTCATATCC AGTAGTCAAG ATGGCCATAA CTGGACTCCA 
TTTCTTCAGA ATGGCAAAGT GAAGGTTTTT CAGGGAAATC AAGACTCCTT CACCCCCGTG 
GTGAATGCTC TAGACCCCCC GCTGTTTACC CGCTTCCTTC GGATTCACCC GCGGAGCTGG 
GCGCACCATA TCGCCCTGAG GCTGGAGCTT TTGGGCTGCG AGGCACAGCA GCAGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024843486.1
CDS	486..7385
Translation	MTPARLRRGRTRSGAPPPSARGGFRFPPRVPKPFPFNTSVMYRKTVFVEFTDQLFNIAKPRPPWMGLLGPTIQAEVYDTVVITFKNMASHPVSLHAIGVSYWKSSEGAAYEDETSQREKEDDKVIPGKSHTYVWQILKENGPTASDPPCLTYSYLSHVDLVKDVNSGLIGALLICREGTLIKERTQTLHKFVLLFAVFDEGKSWHSGKNESLTRVMDSASALHTINGYINRSLPGLIGCHKKSVYWHVIGMGTTPEVHSIFLEGHTFLVRNHRQASLEISPITFLTAQTVLMDCGQFLLFCRISSHQHDGMEAYVKVDSCPEEPRLWMKNNQEEDYDDGLDDSDMDVVRFDGDSAPPFIQMRSVAKKHPKTWVHYIAAEEDDWDYAPSVLTTSDRSYKSLYLNHGPQRIGRKYRKVRFIAYTDGTFKTREAIQHESGILGPLLYGEVGDTLLIIFKNQASRPYNIYPHGITDVSPLHSGRFPKGVKHLKDMPILPGEVFKYKWTVTVEDGPTKSDPRCLTRYYSSFINLEKDLASGLIGPLLICYKESVDQRGNQMMSDKRNVILFSVFDENKSWYLTENIQRFLPNGVQPQDPEFQVSNVMHSINGYVFDSLQLSVCLHEVAYWYILSVGAQIDFLSVFFSGYTFKHKMVYEDTLTLFPFSGETVFMSMENPGLWVLGCHNSDFRNRGMTALLKVDSCDRNVGDYYDTYEDIPTLLSDDNVIEPRSFSKNSRYPSTRQKQFKPTMTPENDMEKIDPQSGEKMQLLTEQSVSSSDLLMLLGQNPTPHRLSLSDLQETRNEATDHLPGPTERIKGPSPSEVAHLRPELHHSGERVFTQEPELPVRLNENLGTTIMAELNKLNLKISSSSNNLMPLPTIPSDKLSAGTKKTGSFGPPNMPVNFNSQLGAIVFGKNLSHVIGSRVPLGLSKEDNNSKLLEAALMNSQESSLGENALSLESDRLFKEERVHGPVSLNKDDALFKVNISLVKTDKAPANSTTNRKTHIDDPTVLIENSTSVWQDIVIDSNTNFQEVTSLIHDELFMHKNTTSFGLSHMSNKTTSSKKREMIHQEKEGPKPLDTKYPDTPFFKTLFLPDSTNVIKRTHDKNSLSSGQRPSPKQLTSSVSENSVKDQNVLSEKNKAVAGEDEFSNNTSLKEMIFPNTKSIVLTNLANVQENDTDNQGKKSQEETEKKETLIPENTVLPQVYTVIGTKNFLKNLFSLSTKQNVRGLDEETYTPGLQDNRSLSDSAKRARIHAAHFSKGRVEANVEGLGNQTKQMVEKSPSTTKLSPIPSQQDAVPHRDKRGLKQSRLPREEVKLERGVTLNDTSTQWSKSMKHLTQGTFTQIEHNKMEKRTITQSFLPDCSLRSHRSIRPTDVTKIPPQHNSSHPPASTYGYNLGASHSGVQETSHFLHGAKKNNLSLAFLTLEVIGSQRKSSTPGKQATNPLMYKKLENMFLLKPGLSEASGKVVHQEDLPTKTSKVSPAHLDLREEIFLQKTQGLVKLNKVYRTGKVPSLKWTTESSEEIPSKLLGLLAWDNQSATLIPKEDWKSLERSHKLTSLKTKDITLPLDPSENYYSIAVTNEGEDKAQREAPWPKQGGTGRVCCQNPPILKRHQREITLTTFQPEPDKTDYDDTLSIETKREDFDIYGEDENQDPRSFQKRTRHYFIAAVERLWDYGMSRSPYALRNRSQNGGVPQFKKVVFEEFTDGSFTQAIYRGQLNEHLGLLGPYIRAEVEDNIMVTFKNQASRPYSFYSSLISYNGDQRQGAEPRKKFVKPNETQSYFWKVQHHMAPTKDEFDCKAWAYFSDVDLEKDLHSGLIGPILICRVDTLSAAHGRQVTIQEFALFFTIFDETKSWYFAENMARNCGAPCHVQPEDPTFQEKYRFHAINGYVMDTLPGLVMAQHQRIRWYLLSMGSNENIHSIHFSGHVFTVRKTEEYKMAVYNLYPGVFETVEMLPSKVGTWRIECLIGEHLQAGMSTLFLVYSKECQIPLGMASGRIRDFQITASGQYGQWAPKLARLHYSGSINAWSTKDPSPWIKVDLLAPMIIHSILTQGARQKFSSLYISQFIIMYSLDGQRWQGYRGNSTGTLMVFFGNVDSSGVKHNIFNPPIIARYIRLHPTHYSIRSTLRMELMGCDLNSCNMPLGMENKAISDTQITASSHLRNMFATWSPSQARLNLQGRTNAWRPQVNNPKEWLQVDFQKTMRVTGITTQGVKSLLTSMYVKEFLISSSQDGHNWTPFLQNGKVKVFQGNQDSFTPVVNALDPPLFTRFLRIHPRSWAHHIALRLELLGCEAQQQY

Target ORF information:

RefSeq Version	XM_024987718.1
Organism	Bos taurus(cattle)
Definition	Bos taurus coagulation factor VIII (F8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024987718.1

ORF Insert Sequence:

ATGACACCCG CGCGGCTTCG CCGCGGGAGG ACGCGGAGTG GCGCGCCGCC GCCGTCTGCG 
CGAGGTGGCT TCCGGTTTCC TCCCAGAGTG CCCAAACCTT TTCCATTCAA CACCTCAGTC 
ATGTACAGAA AGACTGTGTT TGTAGAGTTC ACGGATCAAC TTTTCAACAT CGCCAAGCCC 
AGGCCACCAT GGATGGGTCT GCTGGGTCCA ACTATCCAGG CTGAGGTTTA TGACACCGTG 
GTCATTACAT TTAAGAATAT GGCTTCTCAT CCTGTTAGTC TTCATGCTAT TGGCGTATCC 
TACTGGAAAT CTTCTGAAGG TGCTGCATAT GAGGATGAAA CCAGCCAAAG GGAGAAGGAA 
GATGACAAAG TCATTCCTGG TAAAAGTCAT ACCTACGTCT GGCAGATCCT GAAAGAAAAT 
GGTCCAACAG CCTCTGACCC ACCATGTCTC ACCTACTCAT ATCTTTCTCA TGTGGACCTG 
GTGAAAGACG TGAACTCAGG TCTCATTGGA GCCCTGCTAA TTTGTAGGGA AGGAACTCTG 
ATCAAAGAAA GGACACAGAC CTTGCACAAA TTCGTACTAC TGTTTGCTGT ATTTGATGAA 
GGGAAAAGTT GGCACTCGGG AAAAAATGAG TCCTTGACAC GTGTTATGGA TTCTGCATCT 
GCACTGCACA CCATCAATGG CTATATAAAC AGGTCTCTGC CAGGTCTGAT TGGATGTCAT 
AAGAAATCAG TCTATTGGCA CGTGATTGGA ATGGGCACCA CCCCGGAAGT GCACTCAATT 
TTCCTTGAAG GCCACACATT TCTCGTGAGG AACCATCGCC AGGCTTCCTT GGAGATCTCA 
CCAATAACTT TCCTTACCGC TCAGACAGTC CTGATGGACT GTGGCCAGTT TCTACTGTTT 
TGTCGTATCT CTTCCCACCA ACATGATGGT ATGGAAGCTT ATGTCAAAGT AGATAGTTGC 
CCAGAGGAAC CCCGACTATG GATGAAAAAT AACCAAGAAG AAGATTATGA TGATGGTTTG 
GATGACTCTG ACATGGATGT GGTCAGGTTT GATGGCGACA GTGCGCCTCC CTTTATCCAA 
ATGCGCTCAG TTGCAAAGAA GCATCCTAAA ACCTGGGTCC ACTACATTGC TGCTGAAGAG 
GATGACTGGG ACTATGCCCC CTCGGTCCTC ACCACCAGTG ACAGAAGTTA TAAAAGTCTG 
TATCTGAACC ACGGTCCTCA GCGGATTGGT AGGAAGTACA GAAAAGTACG GTTTATAGCT 
TACACAGATG GAACATTTAA GACTCGTGAA GCTATTCAGC ATGAATCAGG GATCCTGGGG 
CCTTTACTTT ATGGAGAAGT TGGAGACACA CTTTTGATTA TATTTAAGAA TCAAGCAAGC 
CGGCCATATA ACATCTACCC TCATGGAATC ACTGATGTCA GTCCTTTGCA CTCAGGGAGA 
TTTCCAAAAG GTGTGAAACA TTTGAAAGAC ATGCCAATTC TGCCAGGAGA AGTGTTCAAG 
TATAAATGGA CAGTGACTGT AGAAGATGGG CCAACTAAAT CAGATCCTCG GTGTCTGACC 
CGATATTACT CGAGTTTCAT TAACTTAGAG AAAGATCTAG CTTCAGGACT CATTGGCCCC 
CTCCTCATCT GCTACAAAGA ATCTGTAGAT CAAAGAGGAA ACCAGATGAT GTCAGACAAG 
AGAAATGTCA TCCTGTTTTC TGTATTTGAT GAGAATAAAA GTTGGTACCT CACAGAGAAT 
ATTCAGCGCT TCCTCCCCAA TGGAGTACAG CCCCAGGATC CAGAGTTCCA AGTCTCCAAT 
GTCATGCATA GCATCAATGG CTATGTTTTT GATAGCTTGC AGCTGTCGGT TTGTTTGCAT 
GAGGTGGCAT ACTGGTACAT TCTAAGTGTT GGAGCCCAAA TTGACTTCCT CTCTGTCTTC 
TTCTCTGGAT ATACCTTCAA ACACAAAATG GTCTATGAAG ATACACTCAC CCTATTCCCC 
TTTTCAGGAG AAACTGTCTT CATGTCAATG GAAAATCCAG GTCTGTGGGT TCTGGGGTGC 
CACAACTCAG ACTTTCGAAA CAGAGGCATG ACAGCCTTAC TGAAGGTTGA TAGTTGTGAC 
AGGAACGTTG GCGATTATTA TGACACATAT GAAGATATTC CAACCTTGCT GAGTGACGAC 
AATGTCATTG AACCCAGAAG CTTCTCCAAG AATTCAAGGT ACCCTAGCAC TAGGCAAAAG 
CAATTCAAAC CCACCATGAC TCCAGAAAAT GACATGGAGA AGATTGACCC TCAGTCTGGA 
GAGAAAATGC AGCTGCTTAC AGAACAAAGT GTCTCCTCTA GTGATCTGTT GATGCTCTTG 
GGACAGAATC CTACTCCACA CAGATTATCC TTATCTGATC TCCAAGAAAC CAGAAATGAG 
GCTACTGATC ATTTACCTGG ACCAACAGAA AGAATCAAGG GCCCATCCCC ATCTGAAGTG 
GCACATCTTA GACCAGAGCT GCATCACAGT GGGGAAAGAG TATTTACTCA AGAGCCAGAA 
CTGCCAGTAA GATTAAATGA GAATTTGGGA ACAACTATAA TGGCAGAGTT GAACAAACTT 
AATTTAAAAA TTTCAAGTTC ATCAAACAAT CTAATGCCTT TACCAACAAT TCCATCAGAC 
AAGTTGTCAG CAGGTACTAA AAAGACAGGT TCCTTCGGAC CCCCAAATAT GCCAGTTAAT 
TTTAATAGTC AATTAGGTGC CATTGTATTT GGCAAAAATT TATCTCATGT TATTGGATCA 
CGTGTACCTT TGGGCTTGAG TAAAGAAGAT AATAATTCCA AGTTGTTAGA AGCAGCTTTA 
ATGAATAGTC AAGAAAGTTC ACTGGGAGAA AATGCATTAT CACTGGAGAG TGATAGGCTA 
TTTAAAGAGG AAAGAGTTCA TGGACCTGTG TCATTGAACA AAGATGATGC TTTATTTAAA 
GTGAATATCT CTTTGGTAAA GACAGACAAG GCACCAGCTA ACTCAACAAC TAATAGAAAG 
ACTCACATTG ATGACCCAAC AGTATTAATT GAGAATAGTA CATCAGTCTG GCAGGATATT 
GTAATAGACA GTAATACCAA CTTTCAAGAA GTGACTTCTT TGATTCATGA TGAATTGTTT 
ATGCACAAAA ATACTACATC TTTTGGACTA AGCCATATGT CAAATAAAAC TACTTCATCC 
AAAAAAAGGG AAATGATCCA CCAAGAAAAA GAAGGCCCTA AGCCACTAGA TACAAAATAT 
CCAGATACAC CATTCTTCAA GACTCTGTTT TTGCCAGATT CAACAAACGT GATAAAAAGG 
ACCCACGACA AGAACTCCCT AAGCTCTGGG CAAAGGCCCA GTCCAAAGCA ATTAACATCA 
TCAGTATCAG AAAACTCTGT GAAAGATCAG AATGTCTTAT CAGAAAAGAA TAAGGCAGTA 
GCAGGAGAGG ATGAATTTAG CAACAACACC AGCCTCAAAG AGATGATTTT TCCAAATACC 
AAAAGCATAG TTCTTACTAA CTTGGCTAAT GTCCAAGAAA ATGATACAGA CAATCAAGGA 
AAAAAATCCC AGGAAGAAAC AGAAAAGAAA GAGACATTAA TCCCTGAGAA CACAGTTTTG 
CCTCAGGTGT ATACAGTGAT TGGAACTAAG AATTTCCTGA AGAATCTCTT TTCACTGAGC 
ACTAAGCAAA ATGTCAGAGG TTTAGATGAA GAGACATATA CTCCAGGACT TCAAGACAAC 
AGGTCATTAA GTGATTCGGC AAAAAGAGCC AGGATTCATG CAGCCCATTT CTCAAAAGGA 
AGGGTGGAAG CAAACGTGGA AGGCTTAGGG AATCAAACAA AACAAATGGT AGAGAAATCT 
CCAAGCACTA CAAAACTGTC TCCTATCCCA AGTCAGCAGG ATGCAGTTCC TCACCGTGAT 
AAGCGGGGTT TGAAACAATC CAGACTCCCA CGAGAAGAAG TAAAGCTTGA AAGGGGGGTA 
ACTCTGAATG ACACCTCAAC TCAGTGGTCC AAAAGCATGA AACATTTGAC CCAGGGTACC 
TTCACACAGA TAGAACACAA CAAGATGGAG AAAAGGACCA TTACTCAGTC CTTCTTACCA 
GATTGTTCTT TGAGGAGTCA TAGGTCAATT AGACCTACAG ATGTGACCAA GATCCCACCT 
CAACATAACT CTTCTCATCC TCCAGCATCA ACCTATGGTT ATAACTTGGG GGCAAGTCAT 
TCTGGGGTCC AAGAAACCAG TCATTTCTTA CACGGAGCCA AGAAAAATAA TCTTTCTTTA 
GCCTTCCTAA CATTGGAAGT GATCGGAAGT CAAAGAAAGA GCAGCACCCC GGGGAAACAG 
GCCACAAATC CACTCATGTA CAAAAAACTT GAGAACATGT TTCTGTTGAA ACCAGGCTTG 
TCTGAAGCAT CTGGGAAAGT TGTTCATCAG GAAGACTTGC CTACAAAAAC TAGCAAGGTT 
TCTCCTGCCC ACCTGGATCT CAGGGAAGAG ATCTTCCTTC AGAAAACACA GGGACTTGTT 
AAATTGAATA AAGTCTACAG GACTGGAAAA GTGCCATCTC TGAAATGGAC AACAGAAAGC 
TCTGAAGAGA TTCCCTCCAA GCTGCTGGGT CTCCTTGCTT GGGATAACCA GTCAGCTACC 
TTGATACCAA AAGAAGACTG GAAATCCCTA GAGAGGTCAC ACAAACTCAC GTCTTTGAAG 
ACAAAAGATA TCACTTTGCC CCTAGACCCT AGTGAAAACT ATTATTCAAT AGCAGTAACA 
AATGAAGGAG AAGATAAGGC CCAAAGAGAA GCTCCCTGGC CAAAGCAAGG AGGGACTGGA 
AGGGTGTGCT GTCAAAATCC ACCAATCTTG AAACGCCATC AAAGGGAGAT AACCCTTACT 
ACTTTTCAGC CAGAGCCAGA CAAAACTGAC TATGATGATA CTTTGTCAAT TGAAACAAAG 
AGAGAAGATT TTGACATTTA TGGTGAAGAT GAAAATCAGG ACCCCCGCAG CTTTCAAAAG 
AGAACACGCC ACTATTTTAT TGCTGCAGTG GAGCGGCTCT GGGATTATGG GATGAGTAGA 
TCCCCCTACG CACTAAGAAA CAGGTCTCAG AATGGAGGAG TCCCTCAGTT CAAAAAGGTG 
GTATTCGAGG AATTTACTGA TGGCTCCTTT ACTCAGGCCA TATACCGTGG ACAACTAAAT 
GAACACCTGG GACTCTTGGG ACCATATATA AGAGCAGAAG TGGAAGACAA TATCATGGTA 
ACTTTCAAAA ACCAGGCCTC TCGTCCCTAC TCCTTCTATT CTAGCCTTAT TTCTTATAAC 
GGAGATCAGA GACAAGGAGC AGAACCTCGA AAAAAGTTTG TCAAGCCTAA TGAAACCCAA 
AGCTACTTTT GGAAAGTGCA GCACCATATG GCACCCACCA AAGATGAGTT TGACTGCAAA 
GCCTGGGCTT ACTTTTCTGA CGTTGATCTG GAAAAAGATT TGCACTCAGG CTTGATTGGC 
CCCATTCTGA TCTGCCGCGT GGACACGCTG AGTGCTGCTC ATGGGAGACA AGTAACAATA 
CAGGAATTCG CTCTGTTTTT CACCATTTTT GATGAAACCA AGAGCTGGTA CTTTGCCGAA 
AACATGGCAA GGAACTGCGG GGCGCCCTGC CATGTCCAGC CAGAGGACCC TACTTTTCAA 
GAAAAGTATC GCTTCCATGC AATCAATGGC TACGTGATGG ATACACTCCC TGGCTTAGTC 
ATGGCTCAGC ATCAAAGGAT TAGGTGGTAT CTGCTCAGCA TGGGCAGCAA TGAAAATATC 
CATTCCATTC ATTTCAGTGG CCATGTGTTC ACTGTGCGAA AAACGGAGGA GTATAAAATG 
GCGGTCTACA ATCTCTACCC AGGTGTCTTT GAGACGGTGG AAATGCTACC ATCCAAGGTT 
GGGACTTGGC GGATAGAATG TCTTATTGGC GAGCACCTAC AAGCTGGGAT GAGCACTCTC 
TTCCTGGTGT ACAGCAAGGA GTGTCAAATT CCACTGGGAA TGGCTTCTGG ACGCATTAGA 
GATTTTCAGA TTACAGCTTC AGGACAATAT GGACAGTGGG CCCCCAAGCT GGCCAGACTT 
CATTATTCTG GATCAATCAA CGCCTGGAGC ACCAAGGATC CCTCTCCTTG GATCAAGGTG 
GATCTGTTGG CGCCGATGAT TATTCACAGC ATCCTGACTC AGGGTGCCCG GCAGAAGTTC 
TCCAGCCTGT ACATCTCTCA GTTTATCATC ATGTACAGCC TCGATGGACA GCGGTGGCAG 
GGTTATCGGG GGAACTCCAC TGGGACCTTA ATGGTGTTCT TTGGTAATGT GGATTCATCT 
GGAGTAAAAC ACAATATTTT TAACCCTCCA ATTATTGCTA GATATATCCG TTTACACCCA 
ACTCATTACA GCATCCGCAG CACTCTTCGC ATGGAGTTGA TGGGCTGTGA TTTAAACAGT 
TGCAACATGC CACTGGGAAT GGAGAATAAA GCTATATCAG ATACACAGAT TACTGCCTCA 
TCCCACTTAC GCAACATGTT TGCCACCTGG TCTCCTTCAC AAGCCCGACT TAACCTTCAA 
GGGAGGACAA ATGCCTGGAG GCCCCAGGTG AATAATCCAA AAGAGTGGCT ACAAGTGGAC 
TTCCAGAAGA CAATGAGAGT TACAGGAATA ACTACCCAAG GGGTGAAATC TCTGCTTACC 
AGCATGTATG TGAAGGAGTT CCTCATATCC AGTAGTCAAG ATGGCCATAA CTGGACTCCA 
TTTCTTCAGA ATGGCAAAGT GAAGGTTTTT CAGGGAAATC AAGACTCCTT CACCCCCGTG 
GTGAATGCTC TAGACCCCCC GCTGTTTACC CGCTTCCTTC GGATTCACCC GCGGAGCTGG 
GCGCACCATA TCGCCCTGAG GCTGGAGCTT TTGGGCTGCG AGGCACAGCA GCAGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

F8 ( XM_024987719.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024843487.1 Bos taurus coagulation factor VIII (F8), transcript variant X4, mRNA.

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CloneID	OBa238232
Clone ID Related Accession (Same CDS sequence)	XM_024987719.1
Accession Version	XM_024987719.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	coagulation factor VIII isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACAGAA AGACTGTGTT TGTAGAGTTC ACGGATCAAC TTTTCAACAT CGCCAAGCCC 
AGGCCACCAT GGATGGGTCT GCTGGGTCCA ACTATCCAGG CTGAGGTTTA TGACACCGTG 
GTCATTACAT TTAAGAATAT GGCTTCTCAT CCTGTTAGTC TTCATGCTAT TGGCGTATCC 
TACTGGAAAT CTTCTGAAGG TGCTGCATAT GAGGATGAAA CCAGCCAAAG GGAGAAGGAA 
GATGACAAAG TCATTCCTGG TAAAAGTCAT ACCTACGTCT GGCAGATCCT GAAAGAAAAT 
GGTCCAACAG CCTCTGACCC ACCATGTCTC ACCTACTCAT ATCTTTCTCA TGTGGACCTG 
GTGAAAGACG TGAACTCAGG TCTCATTGGA GCCCTGCTAA TTTGTAGGGA AGGAACTCTG 
ATCAAAGAAA GGACACAGAC CTTGCACAAA TTCGTACTAC TGTTTGCTGT ATTTGATGAA 
GGGAAAAGTT GGCACTCGGG AAAAAATGAG TCCTTGACAC GTGTTATGGA TTCTGCATCT 
GCACTGCACA CCATCAATGG CTATATAAAC AGGTCTCTGC CAGGTCTGAT TGGATGTCAT 
AAGAAATCAG TCTATTGGCA CGTGATTGGA ATGGGCACCA CCCCGGAAGT GCACTCAATT 
TTCCTTGAAG GCCACACATT TCTCGTGAGG AACCATCGCC AGGCTTCCTT GGAGATCTCA 
CCAATAACTT TCCTTACCGC TCAGACAGTC CTGATGGACT GTGGCCAGTT TCTACTGTTT 
TGTCGTATCT CTTCCCACCA ACATGATGGT ATGGAAGCTT ATGTCAAAGT AGATAGTTGC 
CCAGAGGAAC CCCGACTATG GATGAAAAAT AACCAAGAAG AAGATTATGA TGATGGTTTG 
GATGACTCTG ACATGGATGT GGTCAGGTTT GATGGCGACA GTGCGCCTCC CTTTATCCAA 
ATGCGCTCAG TTGCAAAGAA GCATCCTAAA ACCTGGGTCC ACTACATTGC TGCTGAAGAG 
GATGACTGGG ACTATGCCCC CTCGGTCCTC ACCACCAGTG ACAGAAGTTA TAAAAGTCTG 
TATCTGAACC ACGGTCCTCA GCGGATTGGT AGGAAGTACA GAAAAGTACG GTTTATAGCT 
TACACAGATG GAACATTTAA GACTCGTGAA GCTATTCAGC ATGAATCAGG GATCCTGGGG 
CCTTTACTTT ATGGAGAAGT TGGAGACACA CTTTTGATTA TATTTAAGAA TCAAGCAAGC 
CGGCCATATA ACATCTACCC TCATGGAATC ACTGATGTCA GTCCTTTGCA CTCAGGGAGA 
TTTCCAAAAG GTGTGAAACA TTTGAAAGAC ATGCCAATTC TGCCAGGAGA AGTGTTCAAG 
TATAAATGGA CAGTGACTGT AGAAGATGGG CCAACTAAAT CAGATCCTCG GTGTCTGACC 
CGATATTACT CGAGTTTCAT TAACTTAGAG AAAGATCTAG CTTCAGGACT CATTGGCCCC 
CTCCTCATCT GCTACAAAGA ATCTGTAGAT CAAAGAGGAA ACCAGATGAT GTCAGACAAG 
AGAAATGTCA TCCTGTTTTC TGTATTTGAT GAGAATAAAA GTTGGTACCT CACAGAGAAT 
ATTCAGCGCT TCCTCCCCAA TGGAGTACAG CCCCAGGATC CAGAGTTCCA AGTCTCCAAT 
GTCATGCATA GCATCAATGG CTATGTTTTT GATAGCTTGC AGCTGTCGGT TTGTTTGCAT 
GAGGTGGCAT ACTGGTACAT TCTAAGTGTT GGAGCCCAAA TTGACTTCCT CTCTGTCTTC 
TTCTCTGGAT ATACCTTCAA ACACAAAATG GTCTATGAAG ATACACTCAC CCTATTCCCC 
TTTTCAGGAG AAACTGTCTT CATGTCAATG GAAAATCCAG GTCTGTGGGT TCTGGGGTGC 
CACAACTCAG ACTTTCGAAA CAGAGGCATG ACAGCCTTAC TGAAGGTTGA TAGTTGTGAC 
AGGAACGTTG GCGATTATTA TGACACATAT GAAGATATTC CAACCTTGCT GAGTGACGAC 
AATGTCATTG AACCCAGAAG CTTCTCCAAG AATTCAAGGT ACCCTAGCAC TAGGCAAAAG 
CAATTCAAAC CCACCATGAC TCCAGAAAAT GACATGGAGA AGATTGACCC TCAGTCTGGA 
GAGAAAATGC AGCTGCTTAC AGAACAAAGT GTCTCCTCTA GTGATCTGTT GATGCTCTTG 
GGACAGAATC CTACTCCACA CAGATTATCC TTATCTGATC TCCAAGAAAC CAGAAATGAG 
GCTACTGATC ATTTACCTGG ACCAACAGAA AGAATCAAGG GCCCATCCCC ATCTGAAGTG 
GCACATCTTA GACCAGAGCT GCATCACAGT GGGGAAAGAG TATTTACTCA AGAGCCAGAA 
CTGCCAGTAA GATTAAATGA GAATTTGGGA ACAACTATAA TGGCAGAGTT GAACAAACTT 
AATTTAAAAA TTTCAAGTTC ATCAAACAAT CTAATGCCTT TACCAACAAT TCCATCAGAC 
AAGTTGTCAG CAGGTACTAA AAAGACAGGT TCCTTCGGAC CCCCAAATAT GCCAGTTAAT 
TTTAATAGTC AATTAGGTGC CATTGTATTT GGCAAAAATT TATCTCATGT TATTGGATCA 
CGTGTACCTT TGGGCTTGAG TAAAGAAGAT AATAATTCCA AGTTGTTAGA AGCAGCTTTA 
ATGAATAGTC AAGAAAGTTC ACTGGGAGAA AATGCATTAT CACTGGAGAG TGATAGGCTA 
TTTAAAGAGG AAAGAGTTCA TGGACCTGTG TCATTGAACA AAGATGATGC TTTATTTAAA 
GTGAATATCT CTTTGGTAAA GACAGACAAG GCACCAGCTA ACTCAACAAC TAATAGAAAG 
ACTCACATTG ATGACCCAAC AGTATTAATT GAGAATAGTA CATCAGTCTG GCAGGATATT 
GTAATAGACA GTAATACCAA CTTTCAAGAA GTGACTTCTT TGATTCATGA TGAATTGTTT 
ATGCACAAAA ATACTACATC TTTTGGACTA AGCCATATGT CAAATAAAAC TACTTCATCC 
AAAAAAAGGG AAATGATCCA CCAAGAAAAA GAAGGCCCTA AGCCACTAGA TACAAAATAT 
CCAGATACAC CATTCTTCAA GACTCTGTTT TTGCCAGATT CAACAAACGT GATAAAAAGG 
ACCCACGACA AGAACTCCCT AAGCTCTGGG CAAAGGCCCA GTCCAAAGCA ATTAACATCA 
TCAGTATCAG AAAACTCTGT GAAAGATCAG AATGTCTTAT CAGAAAAGAA TAAGGCAGTA 
GCAGGAGAGG ATGAATTTAG CAACAACACC AGCCTCAAAG AGATGATTTT TCCAAATACC 
AAAAGCATAG TTCTTACTAA CTTGGCTAAT GTCCAAGAAA ATGATACAGA CAATCAAGGA 
AAAAAATCCC AGGAAGAAAC AGAAAAGAAA GAGACATTAA TCCCTGAGAA CACAGTTTTG 
CCTCAGGTGT ATACAGTGAT TGGAACTAAG AATTTCCTGA AGAATCTCTT TTCACTGAGC 
ACTAAGCAAA ATGTCAGAGG TTTAGATGAA GAGACATATA CTCCAGGACT TCAAGACAAC 
AGGTCATTAA GTGATTCGGC AAAAAGAGCC AGGATTCATG CAGCCCATTT CTCAAAAGGA 
AGGGTGGAAG CAAACGTGGA AGGCTTAGGG AATCAAACAA AACAAATGGT AGAGAAATCT 
CCAAGCACTA CAAAACTGTC TCCTATCCCA AGTCAGCAGG ATGCAGTTCC TCACCGTGAT 
AAGCGGGGTT TGAAACAATC CAGACTCCCA CGAGAAGAAG TAAAGCTTGA AAGGGGGGTA 
ACTCTGAATG ACACCTCAAC TCAGTGGTCC AAAAGCATGA AACATTTGAC CCAGGGTACC 
TTCACACAGA TAGAACACAA CAAGATGGAG AAAAGGACCA TTACTCAGTC CTTCTTACCA 
GATTGTTCTT TGAGGAGTCA TAGGTCAATT AGACCTACAG ATGTGACCAA GATCCCACCT 
CAACATAACT CTTCTCATCC TCCAGCATCA ACCTATGGTT ATAACTTGGG GGCAAGTCAT 
TCTGGGGTCC AAGAAACCAG TCATTTCTTA CACGGAGCCA AGAAAAATAA TCTTTCTTTA 
GCCTTCCTAA CATTGGAAGT GATCGGAAGT CAAAGAAAGA GCAGCACCCC GGGGAAACAG 
GCCACAAATC CACTCATGTA CAAAAAACTT GAGAACATGT TTCTGTTGAA ACCAGGCTTG 
TCTGAAGCAT CTGGGAAAGT TGTTCATCAG GAAGACTTGC CTACAAAAAC TAGCAAGGTT 
TCTCCTGCCC ACCTGGATCT CAGGGAAGAG ATCTTCCTTC AGAAAACACA GGGACTTGTT 
AAATTGAATA AAGTCTACAG GACTGGAAAA GTGCCATCTC TGAAATGGAC AACAGAAAGC 
TCTGAAGAGA TTCCCTCCAA GCTGCTGGGT CTCCTTGCTT GGGATAACCA GTCAGCTACC 
TTGATACCAA AAGAAGACTG GAAATCCCTA GAGAGGTCAC ACAAACTCAC GTCTTTGAAG 
ACAAAAGATA TCACTTTGCC CCTAGACCCT AGTGAAAACT ATTATTCAAT AGCAGTAACA 
AATGAAGGAG AAGATAAGGC CCAAAGAGAA GCTCCCTGGC CAAAGCAAGG AGGGACTGGA 
AGGGTGTGCT GTCAAAATCC ACCAATCTTG AAACGCCATC AAAGGGAGAT AACCCTTACT 
ACTTTTCAGC CAGAGCCAGA CAAAACTGAC TATGATGATA CTTTGTCAAT TGAAACAAAG 
AGAGAAGATT TTGACATTTA TGGTGAAGAT GAAAATCAGG ACCCCCGCAG CTTTCAAAAG 
AGAACACGCC ACTATTTTAT TGCTGCAGTG GAGCGGCTCT GGGATTATGG GATGAGTAGA 
TCCCCCTACG CACTAAGAAA CAGGTCTCAG AATGGAGGAG TCCCTCAGTT CAAAAAGGTG 
GTATTCGAGG AATTTACTGA TGGCTCCTTT ACTCAGGCCA TATACCGTGG ACAACTAAAT 
GAACACCTGG GACTCTTGGG ACCATATATA AGAGCAGAAG TGGAAGACAA TATCATGGTA 
ACTTTCAAAA ACCAGGCCTC TCGTCCCTAC TCCTTCTATT CTAGCCTTAT TTCTTATAAC 
GGAGATCAGA GACAAGGAGC AGAACCTCGA AAAAAGTTTG TCAAGCCTAA TGAAACCCAA 
AGCTACTTTT GGAAAGTGCA GCACCATATG GCACCCACCA AAGATGAGTT TGACTGCAAA 
GCCTGGGCTT ACTTTTCTGA CGTTGATCTG GAAAAAGATT TGCACTCAGG CTTGATTGGC 
CCCATTCTGA TCTGCCGCGT GGACACGCTG AGTGCTGCTC ATGGGAGACA AGTAACAATA 
CAGGAATTCG CTCTGTTTTT CACCATTTTT GATGAAACCA AGAGCTGGTA CTTTGCCGAA 
AACATGGCAA GGAACTGCGG GGCGCCCTGC CATGTCCAGC CAGAGGACCC TACTTTTCAA 
GAAAAGTATC GCTTCCATGC AATCAATGGC TACGTGATGG ATACACTCCC TGGCTTAGTC 
ATGGCTCAGC ATCAAAGGAT TAGGTGGTAT CTGCTCAGCA TGGGCAGCAA TGAAAATATC 
CATTCCATTC ATTTCAGTGG CCATGTGTTC ACTGTGCGAA AAACGGAGGA GTATAAAATG 
GCGGTCTACA ATCTCTACCC AGGTGTCTTT GAGACGGTGG AAATGCTACC ATCCAAGGTT 
GGGACTTGGC GGATAGAATG TCTTATTGGC GAGCACCTAC AAGCTGGGAT GAGCACTCTC 
TTCCTGGTGT ACAGCAAGGA GTGTCAAATT CCACTGGGAA TGGCTTCTGG ACGCATTAGA 
GATTTTCAGA TTACAGCTTC AGGACAATAT GGACAGTGGG CCCCCAAGCT GGCCAGACTT 
CATTATTCTG GATCAATCAA CGCCTGGAGC ACCAAGGATC CCTCTCCTTG GATCAAGGTG 
GATCTGTTGG CGCCGATGAT TATTCACAGC ATCCTGACTC AGGGTGCCCG GCAGAAGTTC 
TCCAGCCTGT ACATCTCTCA GTTTATCATC ATGTACAGCC TCGATGGACA GCGGTGGCAG 
GGTTATCGGG GGAACTCCAC TGGGACCTTA ATGGTGTTCT TTGGTAATGT GGATTCATCT 
GGAGTAAAAC ACAATATTTT TAACCCTCCA ATTATTGCTA GATATATCCG TTTACACCCA 
ACTCATTACA GCATCCGCAG CACTCTTCGC ATGGAGTTGA TGGGCTGTGA TTTAAACAGT 
TGCAACATGC CACTGGGAAT GGAGAATAAA GCTATATCAG ATACACAGAT TACTGCCTCA 
TCCCACTTAC GCAACATGTT TGCCACCTGG TCTCCTTCAC AAGCCCGACT TAACCTTCAA 
GGGAGGACAA ATGCCTGGAG GCCCCAGGTG AATAATCCAA AAGAGTGGCT ACAAGTGGAC 
TTCCAGAAGA CAATGAGAGT TACAGGAATA ACTACCCAAG GGGTGAAATC TCTGCTTACC 
AGCATGTATG TGAAGGAGTT CCTCATATCC AGTAGTCAAG ATGGCCATAA CTGGACTCCA 
TTTCTTCAGA ATGGCAAAGT GAAGGTTTTT CAGGGAAATC AAGACTCCTT CACCCCCGTG 
GTGAATGCTC TAGACCCCCC GCTGTTTACC CGCTTCCTTC GGATTCACCC GCGGAGCTGG 
GCGCACCATA TCGCCCTGAG GCTGGAGCTT TTGGGCTGCG AGGCACAGCA GCAGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024843487.1
CDS	257..7036
Translation	MYRKTVFVEFTDQLFNIAKPRPPWMGLLGPTIQAEVYDTVVITFKNMASHPVSLHAIGVSYWKSSEGAAYEDETSQREKEDDKVIPGKSHTYVWQILKENGPTASDPPCLTYSYLSHVDLVKDVNSGLIGALLICREGTLIKERTQTLHKFVLLFAVFDEGKSWHSGKNESLTRVMDSASALHTINGYINRSLPGLIGCHKKSVYWHVIGMGTTPEVHSIFLEGHTFLVRNHRQASLEISPITFLTAQTVLMDCGQFLLFCRISSHQHDGMEAYVKVDSCPEEPRLWMKNNQEEDYDDGLDDSDMDVVRFDGDSAPPFIQMRSVAKKHPKTWVHYIAAEEDDWDYAPSVLTTSDRSYKSLYLNHGPQRIGRKYRKVRFIAYTDGTFKTREAIQHESGILGPLLYGEVGDTLLIIFKNQASRPYNIYPHGITDVSPLHSGRFPKGVKHLKDMPILPGEVFKYKWTVTVEDGPTKSDPRCLTRYYSSFINLEKDLASGLIGPLLICYKESVDQRGNQMMSDKRNVILFSVFDENKSWYLTENIQRFLPNGVQPQDPEFQVSNVMHSINGYVFDSLQLSVCLHEVAYWYILSVGAQIDFLSVFFSGYTFKHKMVYEDTLTLFPFSGETVFMSMENPGLWVLGCHNSDFRNRGMTALLKVDSCDRNVGDYYDTYEDIPTLLSDDNVIEPRSFSKNSRYPSTRQKQFKPTMTPENDMEKIDPQSGEKMQLLTEQSVSSSDLLMLLGQNPTPHRLSLSDLQETRNEATDHLPGPTERIKGPSPSEVAHLRPELHHSGERVFTQEPELPVRLNENLGTTIMAELNKLNLKISSSSNNLMPLPTIPSDKLSAGTKKTGSFGPPNMPVNFNSQLGAIVFGKNLSHVIGSRVPLGLSKEDNNSKLLEAALMNSQESSLGENALSLESDRLFKEERVHGPVSLNKDDALFKVNISLVKTDKAPANSTTNRKTHIDDPTVLIENSTSVWQDIVIDSNTNFQEVTSLIHDELFMHKNTTSFGLSHMSNKTTSSKKREMIHQEKEGPKPLDTKYPDTPFFKTLFLPDSTNVIKRTHDKNSLSSGQRPSPKQLTSSVSENSVKDQNVLSEKNKAVAGEDEFSNNTSLKEMIFPNTKSIVLTNLANVQENDTDNQGKKSQEETEKKETLIPENTVLPQVYTVIGTKNFLKNLFSLSTKQNVRGLDEETYTPGLQDNRSLSDSAKRARIHAAHFSKGRVEANVEGLGNQTKQMVEKSPSTTKLSPIPSQQDAVPHRDKRGLKQSRLPREEVKLERGVTLNDTSTQWSKSMKHLTQGTFTQIEHNKMEKRTITQSFLPDCSLRSHRSIRPTDVTKIPPQHNSSHPPASTYGYNLGASHSGVQETSHFLHGAKKNNLSLAFLTLEVIGSQRKSSTPGKQATNPLMYKKLENMFLLKPGLSEASGKVVHQEDLPTKTSKVSPAHLDLREEIFLQKTQGLVKLNKVYRTGKVPSLKWTTESSEEIPSKLLGLLAWDNQSATLIPKEDWKSLERSHKLTSLKTKDITLPLDPSENYYSIAVTNEGEDKAQREAPWPKQGGTGRVCCQNPPILKRHQREITLTTFQPEPDKTDYDDTLSIETKREDFDIYGEDENQDPRSFQKRTRHYFIAAVERLWDYGMSRSPYALRNRSQNGGVPQFKKVVFEEFTDGSFTQAIYRGQLNEHLGLLGPYIRAEVEDNIMVTFKNQASRPYSFYSSLISYNGDQRQGAEPRKKFVKPNETQSYFWKVQHHMAPTKDEFDCKAWAYFSDVDLEKDLHSGLIGPILICRVDTLSAAHGRQVTIQEFALFFTIFDETKSWYFAENMARNCGAPCHVQPEDPTFQEKYRFHAINGYVMDTLPGLVMAQHQRIRWYLLSMGSNENIHSIHFSGHVFTVRKTEEYKMAVYNLYPGVFETVEMLPSKVGTWRIECLIGEHLQAGMSTLFLVYSKECQIPLGMASGRIRDFQITASGQYGQWAPKLARLHYSGSINAWSTKDPSPWIKVDLLAPMIIHSILTQGARQKFSSLYISQFIIMYSLDGQRWQGYRGNSTGTLMVFFGNVDSSGVKHNIFNPPIIARYIRLHPTHYSIRSTLRMELMGCDLNSCNMPLGMENKAISDTQITASSHLRNMFATWSPSQARLNLQGRTNAWRPQVNNPKEWLQVDFQKTMRVTGITTQGVKSLLTSMYVKEFLISSSQDGHNWTPFLQNGKVKVFQGNQDSFTPVVNALDPPLFTRFLRIHPRSWAHHIALRLELLGCEAQQQY

Target ORF information:

RefSeq Version	XM_024987719.1
Organism	Bos taurus(cattle)
Definition	Bos taurus coagulation factor VIII (F8), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024987719.1

ORF Insert Sequence:

ATGTACAGAA AGACTGTGTT TGTAGAGTTC ACGGATCAAC TTTTCAACAT CGCCAAGCCC 
AGGCCACCAT GGATGGGTCT GCTGGGTCCA ACTATCCAGG CTGAGGTTTA TGACACCGTG 
GTCATTACAT TTAAGAATAT GGCTTCTCAT CCTGTTAGTC TTCATGCTAT TGGCGTATCC 
TACTGGAAAT CTTCTGAAGG TGCTGCATAT GAGGATGAAA CCAGCCAAAG GGAGAAGGAA 
GATGACAAAG TCATTCCTGG TAAAAGTCAT ACCTACGTCT GGCAGATCCT GAAAGAAAAT 
GGTCCAACAG CCTCTGACCC ACCATGTCTC ACCTACTCAT ATCTTTCTCA TGTGGACCTG 
GTGAAAGACG TGAACTCAGG TCTCATTGGA GCCCTGCTAA TTTGTAGGGA AGGAACTCTG 
ATCAAAGAAA GGACACAGAC CTTGCACAAA TTCGTACTAC TGTTTGCTGT ATTTGATGAA 
GGGAAAAGTT GGCACTCGGG AAAAAATGAG TCCTTGACAC GTGTTATGGA TTCTGCATCT 
GCACTGCACA CCATCAATGG CTATATAAAC AGGTCTCTGC CAGGTCTGAT TGGATGTCAT 
AAGAAATCAG TCTATTGGCA CGTGATTGGA ATGGGCACCA CCCCGGAAGT GCACTCAATT 
TTCCTTGAAG GCCACACATT TCTCGTGAGG AACCATCGCC AGGCTTCCTT GGAGATCTCA 
CCAATAACTT TCCTTACCGC TCAGACAGTC CTGATGGACT GTGGCCAGTT TCTACTGTTT 
TGTCGTATCT CTTCCCACCA ACATGATGGT ATGGAAGCTT ATGTCAAAGT AGATAGTTGC 
CCAGAGGAAC CCCGACTATG GATGAAAAAT AACCAAGAAG AAGATTATGA TGATGGTTTG 
GATGACTCTG ACATGGATGT GGTCAGGTTT GATGGCGACA GTGCGCCTCC CTTTATCCAA 
ATGCGCTCAG TTGCAAAGAA GCATCCTAAA ACCTGGGTCC ACTACATTGC TGCTGAAGAG 
GATGACTGGG ACTATGCCCC CTCGGTCCTC ACCACCAGTG ACAGAAGTTA TAAAAGTCTG 
TATCTGAACC ACGGTCCTCA GCGGATTGGT AGGAAGTACA GAAAAGTACG GTTTATAGCT 
TACACAGATG GAACATTTAA GACTCGTGAA GCTATTCAGC ATGAATCAGG GATCCTGGGG 
CCTTTACTTT ATGGAGAAGT TGGAGACACA CTTTTGATTA TATTTAAGAA TCAAGCAAGC 
CGGCCATATA ACATCTACCC TCATGGAATC ACTGATGTCA GTCCTTTGCA CTCAGGGAGA 
TTTCCAAAAG GTGTGAAACA TTTGAAAGAC ATGCCAATTC TGCCAGGAGA AGTGTTCAAG 
TATAAATGGA CAGTGACTGT AGAAGATGGG CCAACTAAAT CAGATCCTCG GTGTCTGACC 
CGATATTACT CGAGTTTCAT TAACTTAGAG AAAGATCTAG CTTCAGGACT CATTGGCCCC 
CTCCTCATCT GCTACAAAGA ATCTGTAGAT CAAAGAGGAA ACCAGATGAT GTCAGACAAG 
AGAAATGTCA TCCTGTTTTC TGTATTTGAT GAGAATAAAA GTTGGTACCT CACAGAGAAT 
ATTCAGCGCT TCCTCCCCAA TGGAGTACAG CCCCAGGATC CAGAGTTCCA AGTCTCCAAT 
GTCATGCATA GCATCAATGG CTATGTTTTT GATAGCTTGC AGCTGTCGGT TTGTTTGCAT 
GAGGTGGCAT ACTGGTACAT TCTAAGTGTT GGAGCCCAAA TTGACTTCCT CTCTGTCTTC 
TTCTCTGGAT ATACCTTCAA ACACAAAATG GTCTATGAAG ATACACTCAC CCTATTCCCC 
TTTTCAGGAG AAACTGTCTT CATGTCAATG GAAAATCCAG GTCTGTGGGT TCTGGGGTGC 
CACAACTCAG ACTTTCGAAA CAGAGGCATG ACAGCCTTAC TGAAGGTTGA TAGTTGTGAC 
AGGAACGTTG GCGATTATTA TGACACATAT GAAGATATTC CAACCTTGCT GAGTGACGAC 
AATGTCATTG AACCCAGAAG CTTCTCCAAG AATTCAAGGT ACCCTAGCAC TAGGCAAAAG 
CAATTCAAAC CCACCATGAC TCCAGAAAAT GACATGGAGA AGATTGACCC TCAGTCTGGA 
GAGAAAATGC AGCTGCTTAC AGAACAAAGT GTCTCCTCTA GTGATCTGTT GATGCTCTTG 
GGACAGAATC CTACTCCACA CAGATTATCC TTATCTGATC TCCAAGAAAC CAGAAATGAG 
GCTACTGATC ATTTACCTGG ACCAACAGAA AGAATCAAGG GCCCATCCCC ATCTGAAGTG 
GCACATCTTA GACCAGAGCT GCATCACAGT GGGGAAAGAG TATTTACTCA AGAGCCAGAA 
CTGCCAGTAA GATTAAATGA GAATTTGGGA ACAACTATAA TGGCAGAGTT GAACAAACTT 
AATTTAAAAA TTTCAAGTTC ATCAAACAAT CTAATGCCTT TACCAACAAT TCCATCAGAC 
AAGTTGTCAG CAGGTACTAA AAAGACAGGT TCCTTCGGAC CCCCAAATAT GCCAGTTAAT 
TTTAATAGTC AATTAGGTGC CATTGTATTT GGCAAAAATT TATCTCATGT TATTGGATCA 
CGTGTACCTT TGGGCTTGAG TAAAGAAGAT AATAATTCCA AGTTGTTAGA AGCAGCTTTA 
ATGAATAGTC AAGAAAGTTC ACTGGGAGAA AATGCATTAT CACTGGAGAG TGATAGGCTA 
TTTAAAGAGG AAAGAGTTCA TGGACCTGTG TCATTGAACA AAGATGATGC TTTATTTAAA 
GTGAATATCT CTTTGGTAAA GACAGACAAG GCACCAGCTA ACTCAACAAC TAATAGAAAG 
ACTCACATTG ATGACCCAAC AGTATTAATT GAGAATAGTA CATCAGTCTG GCAGGATATT 
GTAATAGACA GTAATACCAA CTTTCAAGAA GTGACTTCTT TGATTCATGA TGAATTGTTT 
ATGCACAAAA ATACTACATC TTTTGGACTA AGCCATATGT CAAATAAAAC TACTTCATCC 
AAAAAAAGGG AAATGATCCA CCAAGAAAAA GAAGGCCCTA AGCCACTAGA TACAAAATAT 
CCAGATACAC CATTCTTCAA GACTCTGTTT TTGCCAGATT CAACAAACGT GATAAAAAGG 
ACCCACGACA AGAACTCCCT AAGCTCTGGG CAAAGGCCCA GTCCAAAGCA ATTAACATCA 
TCAGTATCAG AAAACTCTGT GAAAGATCAG AATGTCTTAT CAGAAAAGAA TAAGGCAGTA 
GCAGGAGAGG ATGAATTTAG CAACAACACC AGCCTCAAAG AGATGATTTT TCCAAATACC 
AAAAGCATAG TTCTTACTAA CTTGGCTAAT GTCCAAGAAA ATGATACAGA CAATCAAGGA 
AAAAAATCCC AGGAAGAAAC AGAAAAGAAA GAGACATTAA TCCCTGAGAA CACAGTTTTG 
CCTCAGGTGT ATACAGTGAT TGGAACTAAG AATTTCCTGA AGAATCTCTT TTCACTGAGC 
ACTAAGCAAA ATGTCAGAGG TTTAGATGAA GAGACATATA CTCCAGGACT TCAAGACAAC 
AGGTCATTAA GTGATTCGGC AAAAAGAGCC AGGATTCATG CAGCCCATTT CTCAAAAGGA 
AGGGTGGAAG CAAACGTGGA AGGCTTAGGG AATCAAACAA AACAAATGGT AGAGAAATCT 
CCAAGCACTA CAAAACTGTC TCCTATCCCA AGTCAGCAGG ATGCAGTTCC TCACCGTGAT 
AAGCGGGGTT TGAAACAATC CAGACTCCCA CGAGAAGAAG TAAAGCTTGA AAGGGGGGTA 
ACTCTGAATG ACACCTCAAC TCAGTGGTCC AAAAGCATGA AACATTTGAC CCAGGGTACC 
TTCACACAGA TAGAACACAA CAAGATGGAG AAAAGGACCA TTACTCAGTC CTTCTTACCA 
GATTGTTCTT TGAGGAGTCA TAGGTCAATT AGACCTACAG ATGTGACCAA GATCCCACCT 
CAACATAACT CTTCTCATCC TCCAGCATCA ACCTATGGTT ATAACTTGGG GGCAAGTCAT 
TCTGGGGTCC AAGAAACCAG TCATTTCTTA CACGGAGCCA AGAAAAATAA TCTTTCTTTA 
GCCTTCCTAA CATTGGAAGT GATCGGAAGT CAAAGAAAGA GCAGCACCCC GGGGAAACAG 
GCCACAAATC CACTCATGTA CAAAAAACTT GAGAACATGT TTCTGTTGAA ACCAGGCTTG 
TCTGAAGCAT CTGGGAAAGT TGTTCATCAG GAAGACTTGC CTACAAAAAC TAGCAAGGTT 
TCTCCTGCCC ACCTGGATCT CAGGGAAGAG ATCTTCCTTC AGAAAACACA GGGACTTGTT 
AAATTGAATA AAGTCTACAG GACTGGAAAA GTGCCATCTC TGAAATGGAC AACAGAAAGC 
TCTGAAGAGA TTCCCTCCAA GCTGCTGGGT CTCCTTGCTT GGGATAACCA GTCAGCTACC 
TTGATACCAA AAGAAGACTG GAAATCCCTA GAGAGGTCAC ACAAACTCAC GTCTTTGAAG 
ACAAAAGATA TCACTTTGCC CCTAGACCCT AGTGAAAACT ATTATTCAAT AGCAGTAACA 
AATGAAGGAG AAGATAAGGC CCAAAGAGAA GCTCCCTGGC CAAAGCAAGG AGGGACTGGA 
AGGGTGTGCT GTCAAAATCC ACCAATCTTG AAACGCCATC AAAGGGAGAT AACCCTTACT 
ACTTTTCAGC CAGAGCCAGA CAAAACTGAC TATGATGATA CTTTGTCAAT TGAAACAAAG 
AGAGAAGATT TTGACATTTA TGGTGAAGAT GAAAATCAGG ACCCCCGCAG CTTTCAAAAG 
AGAACACGCC ACTATTTTAT TGCTGCAGTG GAGCGGCTCT GGGATTATGG GATGAGTAGA 
TCCCCCTACG CACTAAGAAA CAGGTCTCAG AATGGAGGAG TCCCTCAGTT CAAAAAGGTG 
GTATTCGAGG AATTTACTGA TGGCTCCTTT ACTCAGGCCA TATACCGTGG ACAACTAAAT 
GAACACCTGG GACTCTTGGG ACCATATATA AGAGCAGAAG TGGAAGACAA TATCATGGTA 
ACTTTCAAAA ACCAGGCCTC TCGTCCCTAC TCCTTCTATT CTAGCCTTAT TTCTTATAAC 
GGAGATCAGA GACAAGGAGC AGAACCTCGA AAAAAGTTTG TCAAGCCTAA TGAAACCCAA 
AGCTACTTTT GGAAAGTGCA GCACCATATG GCACCCACCA AAGATGAGTT TGACTGCAAA 
GCCTGGGCTT ACTTTTCTGA CGTTGATCTG GAAAAAGATT TGCACTCAGG CTTGATTGGC 
CCCATTCTGA TCTGCCGCGT GGACACGCTG AGTGCTGCTC ATGGGAGACA AGTAACAATA 
CAGGAATTCG CTCTGTTTTT CACCATTTTT GATGAAACCA AGAGCTGGTA CTTTGCCGAA 
AACATGGCAA GGAACTGCGG GGCGCCCTGC CATGTCCAGC CAGAGGACCC TACTTTTCAA 
GAAAAGTATC GCTTCCATGC AATCAATGGC TACGTGATGG ATACACTCCC TGGCTTAGTC 
ATGGCTCAGC ATCAAAGGAT TAGGTGGTAT CTGCTCAGCA TGGGCAGCAA TGAAAATATC 
CATTCCATTC ATTTCAGTGG CCATGTGTTC ACTGTGCGAA AAACGGAGGA GTATAAAATG 
GCGGTCTACA ATCTCTACCC AGGTGTCTTT GAGACGGTGG AAATGCTACC ATCCAAGGTT 
GGGACTTGGC GGATAGAATG TCTTATTGGC GAGCACCTAC AAGCTGGGAT GAGCACTCTC 
TTCCTGGTGT ACAGCAAGGA GTGTCAAATT CCACTGGGAA TGGCTTCTGG ACGCATTAGA 
GATTTTCAGA TTACAGCTTC AGGACAATAT GGACAGTGGG CCCCCAAGCT GGCCAGACTT 
CATTATTCTG GATCAATCAA CGCCTGGAGC ACCAAGGATC CCTCTCCTTG GATCAAGGTG 
GATCTGTTGG CGCCGATGAT TATTCACAGC ATCCTGACTC AGGGTGCCCG GCAGAAGTTC 
TCCAGCCTGT ACATCTCTCA GTTTATCATC ATGTACAGCC TCGATGGACA GCGGTGGCAG 
GGTTATCGGG GGAACTCCAC TGGGACCTTA ATGGTGTTCT TTGGTAATGT GGATTCATCT 
GGAGTAAAAC ACAATATTTT TAACCCTCCA ATTATTGCTA GATATATCCG TTTACACCCA 
ACTCATTACA GCATCCGCAG CACTCTTCGC ATGGAGTTGA TGGGCTGTGA TTTAAACAGT 
TGCAACATGC CACTGGGAAT GGAGAATAAA GCTATATCAG ATACACAGAT TACTGCCTCA 
TCCCACTTAC GCAACATGTT TGCCACCTGG TCTCCTTCAC AAGCCCGACT TAACCTTCAA 
GGGAGGACAA ATGCCTGGAG GCCCCAGGTG AATAATCCAA AAGAGTGGCT ACAAGTGGAC 
TTCCAGAAGA CAATGAGAGT TACAGGAATA ACTACCCAAG GGGTGAAATC TCTGCTTACC 
AGCATGTATG TGAAGGAGTT CCTCATATCC AGTAGTCAAG ATGGCCATAA CTGGACTCCA 
TTTCTTCAGA ATGGCAAAGT GAAGGTTTTT CAGGGAAATC AAGACTCCTT CACCCCCGTG 
GTGAATGCTC TAGACCCCCC GCTGTTTACC CGCTTCCTTC GGATTCACCC GCGGAGCTGG 
GCGCACCATA TCGCCCTGAG GCTGGAGCTT TTGGGCTGCG AGGCACAGCA GCAGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

F8 ( XM_024987720.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024843488.1 Bos taurus coagulation factor VIII (F8), transcript variant X6, mRNA.

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CloneID	OBa238233
Clone ID Related Accession (Same CDS sequence)	XM_024987720.1
Accession Version	XM_024987720.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6216bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	coagulation factor VIII isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACATCA AGCTCTCTAC CTGCTTCTTT CTGTGCCTCT GGCCATGCAG CTTCAGTGCC 
ATCAGAAGAT ACTACCTGGG TGCAGTGGAA CTCTCCTGGG ACTATATGCA AAGTGAACTG 
CTCAGTGAGC TGCATGTGAA CACAAGGTTT CCTCCCAGAG TGCCCAAACC TTTTCCATTC 
AACACCTCAG TCATGTACAG AAAGACTGTG TTTGTAGAGT TCACGGATCA ACTTTTCAAC 
ATCGCCAAGC CCAGGCCACC ATGGATGGGT CTGCTGGGTC CAACTATCCA GGCTGAGGTT 
TATGACACCG TGGTCATTAC ATTTAAGAAT ATGGCTTCTC ATCCTGTTAG TCTTCATGCT 
ATTGGCGTAT CCTACTGGAA ATCTTCTGAA GGTGCTGCAT ATGAGGATGA AACCAGCCAA 
AGGGAGAAGG AAGATGACAA AGTCATTCCT GGTAAAAGTC ATACCTACGT CTGGCAGATC 
CTGAAAGAAA ATGGTCCAAC AGCCTCTGAC CCACCATGTC TCACCTACTC ATATCTTTCT 
CATGTGGACC TGGTGAAAGA CGTGAACTCA GGTCTCATTG GAGCCCTGCT AATTTGTAGG 
GAAGGAACTC TGATCAAAGA AAGGACACAG ACCTTGCACA AATTCGTACT ACTGTTTGCT 
GTATTTGATG AAGGGAAAAG TTGGCACTCG GGAAAAAATG AGTCCTTGAC ACGTGTTATG 
GATTCTGCAT CTGCACTGCA CACCATCAAT GGCTATATAA ACAGGTCTCT GCCAGGTCTG 
ATTGGATGTC ATAAGAAATC AGTCTATTGG CACGTGATTG GAATGGGCAC CACCCCGGAA 
GTGCACTCAA TTTTCCTTGA AGGCCACACA TTTCTCGTGA GGAACCATCG CCAGGCTTCC 
TTGGAGATCT CACCAATAAC TTTCCTTACC GCTCAGACAG TCCTGATGGA CTGTGGCCAG 
TTTCTACTGT TTTGTCGTAT CTCTTCCCAC CAACATGATG GTATGGAAGC TTATGTCAAA 
GTAGATAGTT GCCCAGAGGA ACCCCGACTA TGGATGAAAA ATAACCAAGA AGAAGATTAT 
GATGATGGTT TGGATGACTC TGACATGGAT GTGGTCAGGT TTGATGGCGA CAGTGCGCCT 
CCCTTTATCC AAATGCGCTC AGTTGCAAAG AAGCATCCTA AAACCTGGGT CCACTACATT 
GCTGCTGAAG AGGATGACTG GGACTATGCC CCCTCGGTCC TCACCACCAG TGACAGAAGT 
TATAAAAGTC TGTATCTGAA CCACGGTCCT CAGCGGATTG GTAGGAAGTA CAGAAAAGTA 
CGGTTTATAG CTTACACAGA TGGAACATTT AAGACTCGTG AAGCTATTCA GCATGAATCA 
GGGATCCTGG GGCCTTTACT TTATGGAGAA GTTGGAGACA CACTTTTGAT TATATTTAAG 
AATCAAGCAA GCCGGCCATA TAACATCTAC CCTCATGGAA TCACTGATGT CAGTCCTTTG 
CACTCAGGGA GATTTCCAAA AGGTGTGAAA CATTTGAAAG ACATGCCAAT TCTGCCAGGA 
GAAGTGTTCA AGTATAAATG GACAGTGACT GTAGAAGATG GGCCAACTAA ATCAGATCCT 
CGGTGTCTGA CCCGATATTA CTCGAGTTTC ATTAACTTAG AGAAAGATCT AGCTTCAGGA 
CTCATTGGCC CCCTCCTCAT CTGCTACAAA GAATCTGTAG ATCAAAGAGG AAACCAGATG 
ATGTCAGACA AGAGAAATGT CATCCTGTTT TCTGTATTTG ATGAGAATAA AAGTTGGTAC 
CTCACAGAGA ATATTCAGCG CTTCCTCCCC AATGGAGTAC AGCCCCAGGA TCCAGAGTTC 
CAAGTCTCCA ATGTCATGCA TAGCATCAAT GGCTATGTTT TTGATAGCTT GCAGCTGTCG 
GTTTGTTTGC ATGAGGTGGC ATACTGGTAC ATTCTAAGTG TTGGAGCCCA AATTGACTTC 
CTCTCTGTCT TCTTCTCTGG ATATACCTTC AAACACAAAA TGGTCTATGA AGATACACTC 
ACCCTATTCC CCTTTTCAGG AGAAACTGTC TTCATGTCAA TGGAAAATCC AGGTCTGTGG 
GTTCTGGGGT GCCACAACTC AGACTTTCGA AACAGAGGCA TGACAGCCTT ACTGAAGGTT 
GATAGTTGTG ACAGGAACGT TGGCGATTAT TATGACACAT ATGAAGATAT TCCAACCTTG 
CTGAGTGACG ACAATGTCAT TGAACCCAGA AGCTTCTCCA AGAATTCAAG GTACCCTAGC 
ACTAGGCAAA AGCAATTCAA ACCCACCATG ACTCCAGAAA ATGACATGGA GAAGATTGAC 
CCTCAGTCTG GAGAGAAAAT GCAGCTGCTT ACAGAACAAA GTGTCTCCTC TAGTGATCTG 
TTGATGCTCT TGGGACAGAA TCCTACTCCA CACAGATTAT CCTTATCTGA TCTCCAAGAA 
ACCAGAAATG AGGCTACTGA TCATTTACCT GGACCAACAG AAAGAATCAA GGGCCCATCC 
CCATCTGAAG TGGCACATCT TAGACCAGAG CTGCATCACA GTGGGGAAAG AGTATTTACT 
CAAGAGCCAG AACTGCCAGT AAGATTAAAT GAGAATTTGG GAACAACTAT AATGGCAGAG 
TTGAACAAAC TTAATTTAAA AATTTCAAGT TCATCAAACA ATCTAATGCC TTTACCAACA 
ATTCCATCAG ACAAGTTGTC AGCAGGTACT AAAAAGACAG GTTCCTTCGG ACCCCCAAAT 
ATGCCAGTTA ATTTTAATAG TCAATTAGGT GCCATTGTAT TTGGCAAAAA TTTATCTCAT 
GTTATTGGAT CACGTGTACC TTTGGGCTTG AGTAAAGAAG ATAATAATTC CAAGTTGTTA 
GAAGCAGCTT TAATGAATAG TCAAGAAAGT TCACTGGGAG AAAATGCATT ATCACTGGAG 
AGTGATAGGC TATTTAAAGA GGAAAGAGTT CATGGACCTG TGTCATTGAA CAAAGATGAT 
GCTTTATTTA AAGTGAATAT CTCTTTGGTA AAGACAGACA AGGCACCAGC TAACTCAACA 
ACTAATAGAA AGACTCACAT TGATGACCCA ACAGTATTAA TTGAGAATAG TACATCAGTC 
TGGCAGGATA TTGTAATAGA CAGTAATACC AACTTTCAAG AAGTGACTTC TTTGATTCAT 
GATGAATTGT TTATGCACAA AAATACTACA TCTTTTGGAC TAAGCCATAT GTCAAATAAA 
ACTACTTCAT CCAAAAAAAG GGAAATGATC CACCAAGAAA AAGAAGGCCC TAAGCCACTA 
GATACAAAAT ATCCAGATAC ACCATTCTTC AAGACTCTGT TTTTGCCAGA TTCAACAAAC 
GTGATAAAAA GGACCCACGA CAAGAACTCC CTAAGCTCTG GGCAAAGGCC CAGTCCAAAG 
CAATTAACAT CATCAGTATC AGAAAACTCT GTGAAAGATC AGAATGTCTT ATCAGAAAAG 
AATAAGGCAG TAGCAGGAGA GGATGAATTT AGCAACAACA CCAGCCTCAA AGAGATGATT 
TTTCCAAATA CCAAAAGCAT AGTTCTTACT AACTTGGCTA ATGTCCAAGA AAATGATACA 
GACAATCAAG GAAAAAAATC CCAGGAAGAA ACAGAAAAGA AAGAGACATT AATCCCTGAG 
AACACAGTTT TGCCTCAGGT GTATACAGTG ATTGGAACTA AGAATTTCCT GAAGAATCTC 
TTTTCACTGA GCACTAAGCA AAATGTCAGA GGTTTAGATG AAGAGACATA TACTCCAGGA 
CTTCAAGACA ACAGGTCATT AAGTGATTCG GCAAAAAGAG CCAGGATTCA TGCAGCCCAT 
TTCTCAAAAG GAAGGGTGGA AGCAAACGTG GAAGGCTTAG GGAATCAAAC AAAACAAATG 
GTAGAGAAAT CTCCAAGCAC TACAAAACTG TCTCCTATCC CAAGTCAGCA GGATGCAGTT 
CCTCACCGTG ATAAGCGGGG TTTGAAACAA TCCAGACTCC CACGAGAAGA AGTAAAGCTT 
GAAAGGGGGG TAACTCTGAA TGACACCTCA ACTCAGTGGT CCAAAAGCAT GAAACATTTG 
ACCCAGGGTA CCTTCACACA GATAGAACAC AACAAGATGG AGAAAAGGAC CATTACTCAG 
TCCTTCTTAC CAGATTGTTC TTTGAGGAGT CATAGGTCAA TTAGACCTAC AGATGTGACC 
AAGATCCCAC CTCAACATAA CTCTTCTCAT CCTCCAGCAT CAACCTATGG TTATAACTTG 
GGGGCAAGTC ATTCTGGGGT CCAAGAAACC AGTCATTTCT TACACGGAGC CAAGAAAAAT 
AATCTTTCTT TAGCCTTCCT AACATTGGAA GTGATCGGAA GTCAAAGAAA GAGCAGCACC 
CCGGGGAAAC AGGCCACAAA TCCACTCATG TACAAAAAAC TTGAGAACAT GTTTCTGTTG 
AAACCAGGCT TGTCTGAAGC ATCTGGGAAA GTTGTTCATC AGGAAGACTT GCCTACAAAA 
ACTAGCAAGG TTTCTCCTGC CCACCTGGAT CTCAGGGAAG AGATCTTCCT TCAGAAAACA 
CAGGGACTTG TTAAATTGAA TAAAGTCTAC AGGACTGGAA AAGTGCCATC TCTGAAATGG 
ACAACAGAAA GCTCTGAAGA GATTCCCTCC AAGCTGCTGG GTCTCCTTGC TTGGGATAAC 
CAGTCAGCTA CCTTGATACC AAAAGAAGAC TGGAAATCCC TAGAGAGGTC ACACAAACTC 
ACGTCTTTGA AGACAAAAGA TATCACTTTG CCCCTAGACC CTAGTGAAAA CTATTATTCA 
ATAGCAGTAA CAAATGAAGG AGAAGATAAG GCCCAAAGAG AAGCTCCCTG GCCAAAGCAA 
GGAGGGACTG GAAGGGTGTG CTGTCAAAAT CCACCAATCT TGAAACGCCA TCAAAGGGAG 
ATAACCCTTA CTACTTTTCA GCCAGAGCCA GACAAAACTG ACTATGATGA TACTTTGTCA 
ATTGAAACAA AGAGAGAAGA TTTTGACATT TATGGTGAAG ATGAAAATCA GGACCCCCGC 
AGCTTTCAAA AGAGAACACG CCACTATTTT ATTGCTGCAG TGGAGCGGCT CTGGGATTAT 
GGGATGAGTA GATCCCCCTA CGCACTAAGA AACAGGTCTC AGAATGGAGG AGTCCCTCAG 
TTCAAAAAGG TGGTATTCGA GGAATTTACT GATGGCTCCT TTACTCAGGC CATATACCGT 
GGACAACTAA ATGAACACCT GGGACTCTTG GGACCATATA TAAGAGCAGA AGTGGAAGAC 
AATATCATGG TAACTTTCAA AAACCAGGCC TCTCGTCCCT ACTCCTTCTA TTCTAGCCTT 
ATTTCTTATA ACGGAGATCA GAGACAAGGA GCAGAACCTC GAAAAAAGTT TGTCAAGCCT 
AATGAAACCC AAAGCTACTT TTGGAAAGTG CAGCACCATA TGGCACCCAC CAAAGATGAG 
TTTGACTGCA AAGCCTGGGC TTACTTTTCT GACGTTGATC TGGAAAAAGA TTTGCACTCA 
GGCTTGATTG GCCCCATTCT GATCTGCCGC GTGGACACGC TGAGTGCTGC TCATGGGAGA 
CAAGTAACAA TACAGGAATT CGCTCTGTTT TTCACCATTT TTGATGAAAC CAAGAGCTGG 
TACTTTGCCG AAAACATGGC AAGGAACTGC GGGGCGCCCT GCCATGTCCA GCCAGAGGAC 
CCTACTTTTC AAGAAAAGTA TCGCTTCCAT GCAATCAATG GCTACGTGAT GGATACACTC 
CCTGGCTTAG TCATGGCTCA GCATCAAAGG ATTAGGTGGT ATCTGCTCAG CATGGGCAGC 
AATGAAAATA TCCATTCCAT TCATTTCAGT GGCCATGTGT TCACTGTGCG AAAAACGGAG 
GAGTATAAAA TGGCGGTCTA CAATCTCTAC CCAGGTGTCT TTGAGACGGT GGAAATGCTA 
CCATCCAAGG TTGGGACTTG GCGGATAGAA TGTCTTATTG GCGAGCACCT ACAAGCTGGG 
ATGAGCACTC TCTTCCTGGT GTACAGCAAG GAGTGTCAAA TTCCACTGGG AATGGCTTCT 
GGACGCATTA GAGATTTTCA GATTACAGCT TCAGGACAAT ATGCAAAGGA CAGTGGGCCC 
CCAAGCTGGC CAGACTTCAT TATTCTGGAT CAATCAACGC CTGGAGCACC AAGGATCCCT 
CTCCTTGGAT CAAGGTGGAT CTGTTGGCGC CGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024843488.1
CDS	194..6409
Translation	MHIKLSTCFFLCLWPCSFSAIRRYYLGAVELSWDYMQSELLSELHVNTRFPPRVPKPFPFNTSVMYRKTVFVEFTDQLFNIAKPRPPWMGLLGPTIQAEVYDTVVITFKNMASHPVSLHAIGVSYWKSSEGAAYEDETSQREKEDDKVIPGKSHTYVWQILKENGPTASDPPCLTYSYLSHVDLVKDVNSGLIGALLICREGTLIKERTQTLHKFVLLFAVFDEGKSWHSGKNESLTRVMDSASALHTINGYINRSLPGLIGCHKKSVYWHVIGMGTTPEVHSIFLEGHTFLVRNHRQASLEISPITFLTAQTVLMDCGQFLLFCRISSHQHDGMEAYVKVDSCPEEPRLWMKNNQEEDYDDGLDDSDMDVVRFDGDSAPPFIQMRSVAKKHPKTWVHYIAAEEDDWDYAPSVLTTSDRSYKSLYLNHGPQRIGRKYRKVRFIAYTDGTFKTREAIQHESGILGPLLYGEVGDTLLIIFKNQASRPYNIYPHGITDVSPLHSGRFPKGVKHLKDMPILPGEVFKYKWTVTVEDGPTKSDPRCLTRYYSSFINLEKDLASGLIGPLLICYKESVDQRGNQMMSDKRNVILFSVFDENKSWYLTENIQRFLPNGVQPQDPEFQVSNVMHSINGYVFDSLQLSVCLHEVAYWYILSVGAQIDFLSVFFSGYTFKHKMVYEDTLTLFPFSGETVFMSMENPGLWVLGCHNSDFRNRGMTALLKVDSCDRNVGDYYDTYEDIPTLLSDDNVIEPRSFSKNSRYPSTRQKQFKPTMTPENDMEKIDPQSGEKMQLLTEQSVSSSDLLMLLGQNPTPHRLSLSDLQETRNEATDHLPGPTERIKGPSPSEVAHLRPELHHSGERVFTQEPELPVRLNENLGTTIMAELNKLNLKISSSSNNLMPLPTIPSDKLSAGTKKTGSFGPPNMPVNFNSQLGAIVFGKNLSHVIGSRVPLGLSKEDNNSKLLEAALMNSQESSLGENALSLESDRLFKEERVHGPVSLNKDDALFKVNISLVKTDKAPANSTTNRKTHIDDPTVLIENSTSVWQDIVIDSNTNFQEVTSLIHDELFMHKNTTSFGLSHMSNKTTSSKKREMIHQEKEGPKPLDTKYPDTPFFKTLFLPDSTNVIKRTHDKNSLSSGQRPSPKQLTSSVSENSVKDQNVLSEKNKAVAGEDEFSNNTSLKEMIFPNTKSIVLTNLANVQENDTDNQGKKSQEETEKKETLIPENTVLPQVYTVIGTKNFLKNLFSLSTKQNVRGLDEETYTPGLQDNRSLSDSAKRARIHAAHFSKGRVEANVEGLGNQTKQMVEKSPSTTKLSPIPSQQDAVPHRDKRGLKQSRLPREEVKLERGVTLNDTSTQWSKSMKHLTQGTFTQIEHNKMEKRTITQSFLPDCSLRSHRSIRPTDVTKIPPQHNSSHPPASTYGYNLGASHSGVQETSHFLHGAKKNNLSLAFLTLEVIGSQRKSSTPGKQATNPLMYKKLENMFLLKPGLSEASGKVVHQEDLPTKTSKVSPAHLDLREEIFLQKTQGLVKLNKVYRTGKVPSLKWTTESSEEIPSKLLGLLAWDNQSATLIPKEDWKSLERSHKLTSLKTKDITLPLDPSENYYSIAVTNEGEDKAQREAPWPKQGGTGRVCCQNPPILKRHQREITLTTFQPEPDKTDYDDTLSIETKREDFDIYGEDENQDPRSFQKRTRHYFIAAVERLWDYGMSRSPYALRNRSQNGGVPQFKKVVFEEFTDGSFTQAIYRGQLNEHLGLLGPYIRAEVEDNIMVTFKNQASRPYSFYSSLISYNGDQRQGAEPRKKFVKPNETQSYFWKVQHHMAPTKDEFDCKAWAYFSDVDLEKDLHSGLIGPILICRVDTLSAAHGRQVTIQEFALFFTIFDETKSWYFAENMARNCGAPCHVQPEDPTFQEKYRFHAINGYVMDTLPGLVMAQHQRIRWYLLSMGSNENIHSIHFSGHVFTVRKTEEYKMAVYNLYPGVFETVEMLPSKVGTWRIECLIGEHLQAGMSTLFLVYSKECQIPLGMASGRIRDFQITASGQYAKDSGPPSWPDFIILDQSTPGAPRIPLLGSRWICWRR

Target ORF information:

RefSeq Version	XM_024987720.1
Organism	Bos taurus(cattle)
Definition	Bos taurus coagulation factor VIII (F8), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024987720.1

ORF Insert Sequence:

ATGCACATCA AGCTCTCTAC CTGCTTCTTT CTGTGCCTCT GGCCATGCAG CTTCAGTGCC 
ATCAGAAGAT ACTACCTGGG TGCAGTGGAA CTCTCCTGGG ACTATATGCA AAGTGAACTG 
CTCAGTGAGC TGCATGTGAA CACAAGGTTT CCTCCCAGAG TGCCCAAACC TTTTCCATTC 
AACACCTCAG TCATGTACAG AAAGACTGTG TTTGTAGAGT TCACGGATCA ACTTTTCAAC 
ATCGCCAAGC CCAGGCCACC ATGGATGGGT CTGCTGGGTC CAACTATCCA GGCTGAGGTT 
TATGACACCG TGGTCATTAC ATTTAAGAAT ATGGCTTCTC ATCCTGTTAG TCTTCATGCT 
ATTGGCGTAT CCTACTGGAA ATCTTCTGAA GGTGCTGCAT ATGAGGATGA AACCAGCCAA 
AGGGAGAAGG AAGATGACAA AGTCATTCCT GGTAAAAGTC ATACCTACGT CTGGCAGATC 
CTGAAAGAAA ATGGTCCAAC AGCCTCTGAC CCACCATGTC TCACCTACTC ATATCTTTCT 
CATGTGGACC TGGTGAAAGA CGTGAACTCA GGTCTCATTG GAGCCCTGCT AATTTGTAGG 
GAAGGAACTC TGATCAAAGA AAGGACACAG ACCTTGCACA AATTCGTACT ACTGTTTGCT 
GTATTTGATG AAGGGAAAAG TTGGCACTCG GGAAAAAATG AGTCCTTGAC ACGTGTTATG 
GATTCTGCAT CTGCACTGCA CACCATCAAT GGCTATATAA ACAGGTCTCT GCCAGGTCTG 
ATTGGATGTC ATAAGAAATC AGTCTATTGG CACGTGATTG GAATGGGCAC CACCCCGGAA 
GTGCACTCAA TTTTCCTTGA AGGCCACACA TTTCTCGTGA GGAACCATCG CCAGGCTTCC 
TTGGAGATCT CACCAATAAC TTTCCTTACC GCTCAGACAG TCCTGATGGA CTGTGGCCAG 
TTTCTACTGT TTTGTCGTAT CTCTTCCCAC CAACATGATG GTATGGAAGC TTATGTCAAA 
GTAGATAGTT GCCCAGAGGA ACCCCGACTA TGGATGAAAA ATAACCAAGA AGAAGATTAT 
GATGATGGTT TGGATGACTC TGACATGGAT GTGGTCAGGT TTGATGGCGA CAGTGCGCCT 
CCCTTTATCC AAATGCGCTC AGTTGCAAAG AAGCATCCTA AAACCTGGGT CCACTACATT 
GCTGCTGAAG AGGATGACTG GGACTATGCC CCCTCGGTCC TCACCACCAG TGACAGAAGT 
TATAAAAGTC TGTATCTGAA CCACGGTCCT CAGCGGATTG GTAGGAAGTA CAGAAAAGTA 
CGGTTTATAG CTTACACAGA TGGAACATTT AAGACTCGTG AAGCTATTCA GCATGAATCA 
GGGATCCTGG GGCCTTTACT TTATGGAGAA GTTGGAGACA CACTTTTGAT TATATTTAAG 
AATCAAGCAA GCCGGCCATA TAACATCTAC CCTCATGGAA TCACTGATGT CAGTCCTTTG 
CACTCAGGGA GATTTCCAAA AGGTGTGAAA CATTTGAAAG ACATGCCAAT TCTGCCAGGA 
GAAGTGTTCA AGTATAAATG GACAGTGACT GTAGAAGATG GGCCAACTAA ATCAGATCCT 
CGGTGTCTGA CCCGATATTA CTCGAGTTTC ATTAACTTAG AGAAAGATCT AGCTTCAGGA 
CTCATTGGCC CCCTCCTCAT CTGCTACAAA GAATCTGTAG ATCAAAGAGG AAACCAGATG 
ATGTCAGACA AGAGAAATGT CATCCTGTTT TCTGTATTTG ATGAGAATAA AAGTTGGTAC 
CTCACAGAGA ATATTCAGCG CTTCCTCCCC AATGGAGTAC AGCCCCAGGA TCCAGAGTTC 
CAAGTCTCCA ATGTCATGCA TAGCATCAAT GGCTATGTTT TTGATAGCTT GCAGCTGTCG 
GTTTGTTTGC ATGAGGTGGC ATACTGGTAC ATTCTAAGTG TTGGAGCCCA AATTGACTTC 
CTCTCTGTCT TCTTCTCTGG ATATACCTTC AAACACAAAA TGGTCTATGA AGATACACTC 
ACCCTATTCC CCTTTTCAGG AGAAACTGTC TTCATGTCAA TGGAAAATCC AGGTCTGTGG 
GTTCTGGGGT GCCACAACTC AGACTTTCGA AACAGAGGCA TGACAGCCTT ACTGAAGGTT 
GATAGTTGTG ACAGGAACGT TGGCGATTAT TATGACACAT ATGAAGATAT TCCAACCTTG 
CTGAGTGACG ACAATGTCAT TGAACCCAGA AGCTTCTCCA AGAATTCAAG GTACCCTAGC 
ACTAGGCAAA AGCAATTCAA ACCCACCATG ACTCCAGAAA ATGACATGGA GAAGATTGAC 
CCTCAGTCTG GAGAGAAAAT GCAGCTGCTT ACAGAACAAA GTGTCTCCTC TAGTGATCTG 
TTGATGCTCT TGGGACAGAA TCCTACTCCA CACAGATTAT CCTTATCTGA TCTCCAAGAA 
ACCAGAAATG AGGCTACTGA TCATTTACCT GGACCAACAG AAAGAATCAA GGGCCCATCC 
CCATCTGAAG TGGCACATCT TAGACCAGAG CTGCATCACA GTGGGGAAAG AGTATTTACT 
CAAGAGCCAG AACTGCCAGT AAGATTAAAT GAGAATTTGG GAACAACTAT AATGGCAGAG 
TTGAACAAAC TTAATTTAAA AATTTCAAGT TCATCAAACA ATCTAATGCC TTTACCAACA 
ATTCCATCAG ACAAGTTGTC AGCAGGTACT AAAAAGACAG GTTCCTTCGG ACCCCCAAAT 
ATGCCAGTTA ATTTTAATAG TCAATTAGGT GCCATTGTAT TTGGCAAAAA TTTATCTCAT 
GTTATTGGAT CACGTGTACC TTTGGGCTTG AGTAAAGAAG ATAATAATTC CAAGTTGTTA 
GAAGCAGCTT TAATGAATAG TCAAGAAAGT TCACTGGGAG AAAATGCATT ATCACTGGAG 
AGTGATAGGC TATTTAAAGA GGAAAGAGTT CATGGACCTG TGTCATTGAA CAAAGATGAT 
GCTTTATTTA AAGTGAATAT CTCTTTGGTA AAGACAGACA AGGCACCAGC TAACTCAACA 
ACTAATAGAA AGACTCACAT TGATGACCCA ACAGTATTAA TTGAGAATAG TACATCAGTC 
TGGCAGGATA TTGTAATAGA CAGTAATACC AACTTTCAAG AAGTGACTTC TTTGATTCAT 
GATGAATTGT TTATGCACAA AAATACTACA TCTTTTGGAC TAAGCCATAT GTCAAATAAA 
ACTACTTCAT CCAAAAAAAG GGAAATGATC CACCAAGAAA AAGAAGGCCC TAAGCCACTA 
GATACAAAAT ATCCAGATAC ACCATTCTTC AAGACTCTGT TTTTGCCAGA TTCAACAAAC 
GTGATAAAAA GGACCCACGA CAAGAACTCC CTAAGCTCTG GGCAAAGGCC CAGTCCAAAG 
CAATTAACAT CATCAGTATC AGAAAACTCT GTGAAAGATC AGAATGTCTT ATCAGAAAAG 
AATAAGGCAG TAGCAGGAGA GGATGAATTT AGCAACAACA CCAGCCTCAA AGAGATGATT 
TTTCCAAATA CCAAAAGCAT AGTTCTTACT AACTTGGCTA ATGTCCAAGA AAATGATACA 
GACAATCAAG GAAAAAAATC CCAGGAAGAA ACAGAAAAGA AAGAGACATT AATCCCTGAG 
AACACAGTTT TGCCTCAGGT GTATACAGTG ATTGGAACTA AGAATTTCCT GAAGAATCTC 
TTTTCACTGA GCACTAAGCA AAATGTCAGA GGTTTAGATG AAGAGACATA TACTCCAGGA 
CTTCAAGACA ACAGGTCATT AAGTGATTCG GCAAAAAGAG CCAGGATTCA TGCAGCCCAT 
TTCTCAAAAG GAAGGGTGGA AGCAAACGTG GAAGGCTTAG GGAATCAAAC AAAACAAATG 
GTAGAGAAAT CTCCAAGCAC TACAAAACTG TCTCCTATCC CAAGTCAGCA GGATGCAGTT 
CCTCACCGTG ATAAGCGGGG TTTGAAACAA TCCAGACTCC CACGAGAAGA AGTAAAGCTT 
GAAAGGGGGG TAACTCTGAA TGACACCTCA ACTCAGTGGT CCAAAAGCAT GAAACATTTG 
ACCCAGGGTA CCTTCACACA GATAGAACAC AACAAGATGG AGAAAAGGAC CATTACTCAG 
TCCTTCTTAC CAGATTGTTC TTTGAGGAGT CATAGGTCAA TTAGACCTAC AGATGTGACC 
AAGATCCCAC CTCAACATAA CTCTTCTCAT CCTCCAGCAT CAACCTATGG TTATAACTTG 
GGGGCAAGTC ATTCTGGGGT CCAAGAAACC AGTCATTTCT TACACGGAGC CAAGAAAAAT 
AATCTTTCTT TAGCCTTCCT AACATTGGAA GTGATCGGAA GTCAAAGAAA GAGCAGCACC 
CCGGGGAAAC AGGCCACAAA TCCACTCATG TACAAAAAAC TTGAGAACAT GTTTCTGTTG 
AAACCAGGCT TGTCTGAAGC ATCTGGGAAA GTTGTTCATC AGGAAGACTT GCCTACAAAA 
ACTAGCAAGG TTTCTCCTGC CCACCTGGAT CTCAGGGAAG AGATCTTCCT TCAGAAAACA 
CAGGGACTTG TTAAATTGAA TAAAGTCTAC AGGACTGGAA AAGTGCCATC TCTGAAATGG 
ACAACAGAAA GCTCTGAAGA GATTCCCTCC AAGCTGCTGG GTCTCCTTGC TTGGGATAAC 
CAGTCAGCTA CCTTGATACC AAAAGAAGAC TGGAAATCCC TAGAGAGGTC ACACAAACTC 
ACGTCTTTGA AGACAAAAGA TATCACTTTG CCCCTAGACC CTAGTGAAAA CTATTATTCA 
ATAGCAGTAA CAAATGAAGG AGAAGATAAG GCCCAAAGAG AAGCTCCCTG GCCAAAGCAA 
GGAGGGACTG GAAGGGTGTG CTGTCAAAAT CCACCAATCT TGAAACGCCA TCAAAGGGAG 
ATAACCCTTA CTACTTTTCA GCCAGAGCCA GACAAAACTG ACTATGATGA TACTTTGTCA 
ATTGAAACAA AGAGAGAAGA TTTTGACATT TATGGTGAAG ATGAAAATCA GGACCCCCGC 
AGCTTTCAAA AGAGAACACG CCACTATTTT ATTGCTGCAG TGGAGCGGCT CTGGGATTAT 
GGGATGAGTA GATCCCCCTA CGCACTAAGA AACAGGTCTC AGAATGGAGG AGTCCCTCAG 
TTCAAAAAGG TGGTATTCGA GGAATTTACT GATGGCTCCT TTACTCAGGC CATATACCGT 
GGACAACTAA ATGAACACCT GGGACTCTTG GGACCATATA TAAGAGCAGA AGTGGAAGAC 
AATATCATGG TAACTTTCAA AAACCAGGCC TCTCGTCCCT ACTCCTTCTA TTCTAGCCTT 
ATTTCTTATA ACGGAGATCA GAGACAAGGA GCAGAACCTC GAAAAAAGTT TGTCAAGCCT 
AATGAAACCC AAAGCTACTT TTGGAAAGTG CAGCACCATA TGGCACCCAC CAAAGATGAG 
TTTGACTGCA AAGCCTGGGC TTACTTTTCT GACGTTGATC TGGAAAAAGA TTTGCACTCA 
GGCTTGATTG GCCCCATTCT GATCTGCCGC GTGGACACGC TGAGTGCTGC TCATGGGAGA 
CAAGTAACAA TACAGGAATT CGCTCTGTTT TTCACCATTT TTGATGAAAC CAAGAGCTGG 
TACTTTGCCG AAAACATGGC AAGGAACTGC GGGGCGCCCT GCCATGTCCA GCCAGAGGAC 
CCTACTTTTC AAGAAAAGTA TCGCTTCCAT GCAATCAATG GCTACGTGAT GGATACACTC 
CCTGGCTTAG TCATGGCTCA GCATCAAAGG ATTAGGTGGT ATCTGCTCAG CATGGGCAGC 
AATGAAAATA TCCATTCCAT TCATTTCAGT GGCCATGTGT TCACTGTGCG AAAAACGGAG 
GAGTATAAAA TGGCGGTCTA CAATCTCTAC CCAGGTGTCT TTGAGACGGT GGAAATGCTA 
CCATCCAAGG TTGGGACTTG GCGGATAGAA TGTCTTATTG GCGAGCACCT ACAAGCTGGG 
ATGAGCACTC TCTTCCTGGT GTACAGCAAG GAGTGTCAAA TTCCACTGGG AATGGCTTCT 
GGACGCATTA GAGATTTTCA GATTACAGCT TCAGGACAAT ATGCAAAGGA CAGTGGGCCC 
CCAAGCTGGC CAGACTTCAT TATTCTGGAT CAATCAACGC CTGGAGCACC AAGGATCCCT 
CTCCTTGGAT CAAGGTGGAT CTGTTGGCGC CGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

F8 ( XM_005227639.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005227696.1 Bos taurus coagulation factor VIII (F8), transcript variant X1, mRNA.

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CloneID	OBa35243
Clone ID Related Accession (Same CDS sequence)	XM_005227639.3 , XM_005227639.4 , XM_024987717.1
Accession Version	XM_005227639.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	coagulation factor VIII isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005227639.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACATCA AGCTCTCTAC CTGCTTCTTT CTGTGCCTCT GGCCATGCAG CTTCAGTGCC 
ATCAGAAGAT ACTACCTGGG TGCAGTGGAA CTCTCCTGGG ACTATATGCA AAGTGAACTG 
CTCAGTGAGC TGCATGTGAA CACAAGGTTT CCTCCCAGAG TGCCCAAACC TTTTCCATTC 
AACACCTCAG TCATGTACAG AAAGACTGTG TTTGTAGAGT TCACGGATCA ACTTTTCAAC 
ATCGCCAAGC CCAGGCCACC ATGGATGGGT CTGCTGGGTC CAACTATCCA GGCTGAGGTT 
TATGACACCG TGGTCATTAC ATTTAAGAAT ATGGCTTCTC ATCCTGTTAG TCTTCATGCT 
ATTGGCGTAT CCTACTGGAA ATCTTCTGAA GGTGCTGCAT ATGAGGATGA AACCAGCCAA 
AGGGAGAAGG AAGATGACAA AGTCATTCCT GGTAAAAGTC ATACCTACGT CTGGCAGATC 
CTGAAAGAAA ATGGTCCAAC AGCCTCTGAC CCACCATGTC TCACCTACTC ATATCTTTCT 
CATGTGGACC TGGTGAAAGA CGTGAACTCA GGTCTCATTG GAGCCCTGCT AATTTGTAGG 
GAAGGAACTC TGATCAAAGA AAGGACACAG ACCTTGCACA AATTCGTACT ACTGTTTGCT 
GTATTTGATG AAGGGAAAAG TTGGCACTCG GGAAAAAATG AGTCCTTGAC ACGTGTTATG 
GATTCTGCAT CTGCACTGCA CACCATCAAT GGCTATATAA ACAGGTCTCT GCCAGGTCTG 
ATTGGATGTC ATAAGAAATC AGTCTATTGG CACGTGATTG GAATGGGCAC CACCCCGGAA 
GTGCACTCAA TTTTCCTTGA AGGCCACACA TTTCTCGTGA GGAACCATCG CCAGGCTTCC 
TTGGAGATCT CACCAATAAC TTTCCTTACC GCTCAGACAG TCCTGATGGA CTGTGGCCAG 
TTTCTACTGT TTTGTCGTAT CTCTTCCCAC CAACATGATG GTATGGAAGC TTATGTCAAA 
GTAGATAGTT GCCCAGAGGA ACCCCGACTA TGGATGAAAA ATAACCAAGA AGAAGATTAT 
GATGATGGTT TGGATGACTC TGACATGGAT GTGGTCAGGT TTGATGGCGA CAGTGCGCCT 
CCCTTTATCC AAATGCGCTC AGTTGCAAAG AAGCATCCTA AAACCTGGGT CCACTACATT 
GCTGCTGAAG AGGATGACTG GGACTATGCC CCCTCGGTCC TCACCACCAG TGACAGAAGT 
TATAAAAGTC TGTATCTGAA CCACGGTCCT CAGCGGATTG GTAGGAAGTA CAGAAAAGTA 
CGGTTTATAG CTTACACAGA TGGAACATTT AAGACTCGTG AAGCTATTCA GCATGAATCA 
GGGATCCTGG GGCCTTTACT TTATGGAGAA GTTGGAGACA CACTTTTGAT TATATTTAAG 
AATCAAGCAA GCCGGCCATA TAACATCTAC CCTCATGGAA TCACTGATGT CAGTCCTTTG 
CACTCAGGGA GATTTCCAAA AGGTGTGAAA CATTTGAAAG ACATGCCAAT TCTGCCAGGA 
GAAGTGTTCA AGTATAAATG GACAGTGACT GTAGAAGATG GGCCAACTAA ATCAGATCCT 
CGGTGTCTGA CCCGATATTA CTCGAGTTTC ATTAACTTAG AGAAAGATCT AGCTTCAGGA 
CTCATTGGCC CCCTCCTCAT CTGCTACAAA GAATCTGTAG ATCAAAGAGG AAACCAGATG 
ATGTCAGACA AGAGAAATGT CATCCTGTTT TCTGTATTTG ATGAGAATAA AAGTTGGTAC 
CTCACAGAGA ATATTCAGCG CTTCCTCCCC AATGGAGTAC AGCCCCAGGA TCCAGAGTTC 
CAAGTCTCCA ATGTCATGCA TAGCATCAAT GGCTATGTTT TTGATAGCTT GCAGCTGTCG 
GTTTGTTTGC ATGAGGTGGC ATACTGGTAC ATTCTAAGTG TTGGAGCCCA AATTGACTTC 
CTCTCTGTCT TCTTCTCTGG ATATACCTTC AAACACAAAA TGGTCTATGA AGATACACTC 
ACCCTATTCC CCTTTTCAGG AGAAACTGTC TTCATGTCAA TGGAAAATCC AGGTCTGTGG 
GTTCTGGGGT GCCACAACTC AGACTTTCGA AACAGAGGCA TGACAGCCTT ACTGAAGGTT 
GATAGTTGTG ACAGGAACGT TGGCGATTAT TATGACACAT ATGAAGATAT TCCAACCTTG 
CTGAGTGACG ACAATGTCAT TGAACCCAGA AGCTTCTCCA AGAATTCAAG GTACCCTAGC 
ACTAGGCAAA AGCAATTCAA ACCCACCATG ACTCCAGAAA ATGACATGGA GAAGATTGAC 
CCTCAGTCTG GAGAGAAAAT GCAGCTGCTT ACAGAACAAA GTGTCTCCTC TAGTGATCTG 
TTGATGCTCT TGGGACAGAA TCCTACTCCA CACAGATTAT CCTTATCTGA TCTCCAAGAA 
ACCAGAAATG AGGCTACTGA TCATTTACCT GGACCAACAG AAAGAATCAA GGGCCCATCC 
CCATCTGAAG TGGCACATCT TAGACCAGAG CTGCATCACA GTGGGGAAAG AGTATTTACT 
CAAGAGCCAG AACTGCCAGT AAGATTAAAT GAGAATTTGG GAACAACTAT AATGGCAGAG 
TTGAACAAAC TTAATTTAAA AATTTCAAGT TCATCAAACA ATCTAATGCC TTTACCAACA 
ATTCCATCAG ACAAGTTGTC AGCAGGTACT AAAAAGACAG GTTCCTTCGG ACCCCCAAAT 
ATGCCAGTTA ATTTTAATAG TCAATTAGGT GCCATTGTAT TTGGCAAAAA TTTATCTCAT 
GTTATTGGAT CACGTGTACC TTTGGGCTTG AGTAAAGAAG ATAATAATTC CAAGTTGTTA 
GAAGCAGCTT TAATGAATAG TCAAGAAAGT TCACTGGGAG AAAATGCATT ATCACTGGAG 
AGTGATAGGC TATTTAAAGA GGAAAGAGTT CATGGACCTG TGTCATTGAA CAAAGATGAT 
GCTTTATTTA AAGTGAATAT CTCTTTGGTA AAGACAGACA AGGCACCAGC TAACTCAACA 
ACTAATAGAA AGACTCACAT TGATGACCCA ACAGTATTAA TTGAGAATAG TACATCAGTC 
TGGCAGGATA TTGTAATAGA CAGTAATACC AACTTTCAAG AAGTGACTTC TTTGATTCAT 
GATGAATTGT TTATGCACAA AAATACTACA TCTTTTGGAC TAAGCCATAT GTCAAATAAA 
ACTACTTCAT CCAAAAAAAG GGAAATGATC CACCAAGAAA AAGAAGGCCC TAAGCCACTA 
GATACAAAAT ATCCAGATAC ACCATTCTTC AAGACTCTGT TTTTGCCAGA TTCAACAAAC 
GTGATAAAAA GGACCCACGA CAAGAACTCC CTAAGCTCTG GGCAAAGGCC CAGTCCAAAG 
CAATTAACAT CATCAGTATC AGAAAACTCT GTGAAAGATC AGAATGTCTT ATCAGAAAAG 
AATAAGGCAG TAGCAGGAGA GGATGAATTT AGCAACAACA CCAGCCTCAA AGAGATGATT 
TTTCCAAATA CCAAAAGCAT AGTTCTTACT AACTTGGCTA ATGTCCAAGA AAATGATACA 
GACAATCAAG GAAAAAAATC CCAGGAAGAA ACAGAAAAGA AAGAGACATT AATCCCTGAG 
AACACAGTTT TGCCTCAGGT GTATACAGTG ATTGGAACTA AGAATTTCCT GAAGAATCTC 
TTTTCACTGA GCACTAAGCA AAATGTCAGA GGTTTAGATG AAGAGACATA TACTCCAGGA 
CTTCAAGACA ACAGGTCATT AAGTGATTCG GCAAAAAGAG CCAGGATTCA TGCAGCCCAT 
TTCTCAAAAG GAAGGGTGGA AGCAAACGTG GAAGGCTTAG GGAATCAAAC AAAACAAATG 
GTAGAGAAAT CTCCAAGCAC TACAAAACTG TCTCCTATCC CAAGTCAGCA GGATGCAGTT 
CCTCACCGTG ATAAGCGGGG TTTGAAACAA TCCAGACTCC CACGAGAAGA AGTAAAGCTT 
GAAAGGGGGG TAACTCTGAA TGACACCTCA ACTCAGTGGT CCAAAAGCAT GAAACATTTG 
ACCCAGGGTA CCTTCACACA GATAGAACAC AACAAGATGG AGAAAAGGAC CATTACTCAG 
TCCTTCTTAC CAGATTGTTC TTTGAGGAGT CATAGGTCAA TTAGACCTAC AGATGTGACC 
AAGATCCCAC CTCAACATAA CTCTTCTCAT CCTCCAGCAT CAACCTATGG TTATAACTTG 
GGGGCAAGTC ATTCTGGGGT CCAAGAAACC AGTCATTTCT TACACGGAGC CAAGAAAAAT 
AATCTTTCTT TAGCCTTCCT AACATTGGAA GTGATCGGAA GTCAAAGAAA GAGCAGCACC 
CCGGGGAAAC AGGCCACAAA TCCACTCATG TACAAAAAAC TTGAGAACAT GTTTCTGTTG 
AAACCAGGCT TGTCTGAAGC ATCTGGGAAA GTTGTTCATC AGGAAGACTT GCCTACAAAA 
ACTAGCAAGG TTTCTCCTGC CCACCTGGAT CTCAGGGAAG AGATCTTCCT TCAGAAAACA 
CAGGGACTTG TTAAATTGAA TAAAGTCTAC AGGACTGGAA AAGTGCCATC TCTGAAATGG 
ACAACAGAAA GCTCTGAAGA GATTCCCTCC AAGCTGCTGG GTCTCCTTGC TTGGGATAAC 
CAGTCAGCTA CCTTGATACC AAAAGAAGAC TGGAAATCCC TAGAGAGGTC ACACAAACTC 
ACGTCTTTGA AGACAAAAGA TATCACTTTG CCCCTAGACC CTAGTGAAAA CTATTATTCA 
ATAGCAGTAA CAAATGAAGG AGAAGATAAG GCCCAAAGAG AAGCTCCCTG GCCAAAGCAA 
GGAGGGACTG GAAGGGTGTG CTGTCAAAAT CCACCAATCT TGAAACGCCA TCAAAGGGAG 
ATAACCCTTA CTACTTTTCA GCCAGAGCCA GACAAAACTG ACTATGATGA TACTTTGTCA 
ATTGAAACAA AGAGAGAAGA TTTTGACATT TATGGTGAAG ATGAAAATCA GGACCCCCGC 
AGCTTTCAAA AGAGAACACG CCACTATTTT ATTGCTGCAG TGGAGCGGCT CTGGGATTAT 
GGGATGAGTA GATCCCCCTA CGCACTAAGA AACAGGTCTC AGAATGGAGG AGTCCCTCAG 
TTCAAAAAGG TGGTATTCGA GGAATTTACT GATGGCTCCT TTACTCAGGC CATATACCGT 
GGACAACTAA ATGAACACCT GGGACTCTTG GGACCATATA TAAGAGCAGA AGTGGAAGAC 
AATATCATGG TAACTTTCAA AAACCAGGCC TCTCGTCCCT ACTCCTTCTA TTCTAGCCTT 
ATTTCTTATA ACGGAGATCA GAGACAAGGA GCAGAACCTC GAAAAAAGTT TGTCAAGCCT 
AATGAAACCC AAAGCTACTT TTGGAAAGTG CAGCACCATA TGGCACCCAC CAAAGATGAG 
TTTGACTGCA AAGCCTGGGC TTACTTTTCT GACGTTGATC TGGAAAAAGA TTTGCACTCA 
GGCTTGATTG GCCCCATTCT GATCTGCCGC GTGGACACGC TGAGTGCTGC TCATGGGAGA 
CAAGTAACAA TACAGGAATT CGCTCTGTTT TTCACCATTT TTGATGAAAC CAAGAGCTGG 
TACTTTGCCG AAAACATGGC AAGGAACTGC GGGGCGCCCT GCCATGTCCA GCCAGAGGAC 
CCTACTTTTC AAGAAAAGTA TCGCTTCCAT GCAATCAATG GCTACGTGAT GGATACACTC 
CCTGGCTTAG TCATGGCTCA GCATCAAAGG ATTAGGTGGT ATCTGCTCAG CATGGGCAGC 
AATGAAAATA TCCATTCCAT TCATTTCAGT GGCCATGTGT TCACTGTGCG AAAAACGGAG 
GAGTATAAAA TGGCGGTCTA CAATCTCTAC CCAGGTGTCT TTGAGACGGT GGAAATGCTA 
CCATCCAAGG TTGGGACTTG GCGGATAGAA TGTCTTATTG GCGAGCACCT ACAAGCTGGG 
ATGAGCACTC TCTTCCTGGT GTACAGCAAG GAGTGTCAAA TTCCACTGGG AATGGCTTCT 
GGACGCATTA GAGATTTTCA GATTACAGCT TCAGGACAAT ATGGACAGTG GGCCCCCAAG 
CTGGCCAGAC TTCATTATTC TGGATCAATC AACGCCTGGA GCACCAAGGA TCCCTCTCCT 
TGGATCAAGG TGGATCTGTT GGCGCCGATG ATTATTCACA GCATCCTGAC TCAGGGTGCC 
CGGCAGAAGT TCTCCAGCCT GTACATCTCT CAGTTTATCA TCATGTACAG CCTCGATGGA 
CAGCGGTGGC AGGGTTATCG GGGGAACTCC ACTGGGACCT TAATGGTGTT CTTTGGTAAT 
GTGGATTCAT CTGGAGTAAA ACACAATATT TTTAACCCTC CAATTATTGC TAGATATATC 
CGTTTACACC CAACTCATTA CAGCATCCGC AGCACTCTTC GCATGGAGTT GATGGGCTGT 
GATTTAAACA GTTGCAACAT GCCACTGGGA ATGGAGAATA AAGCTATATC AGATACACAG 
ATTACTGCCT CATCCCACTT ACGCAACATG TTTGCCACCT GGTCTCCTTC ACAAGCCCGA 
CTTAACCTTC AAGGGAGGAC AAATGCCTGG AGGCCCCAGG TGAATAATCC AAAAGAGTGG 
CTACAAGTGG ACTTCCAGAA GACAATGAGA GTTACAGGAA TAACTACCCA AGGGGTGAAA 
TCTCTGCTTA CCAGCATGTA TGTGAAGGAG TTCCTCATAT CCAGTAGTCA AGATGGCCAT 
AACTGGACTC CATTTCTTCA GAATGGCAAA GTGAAGGTTT TTCAGGGAAA TCAAGACTCC 
TTCACCCCCG TGGTGAATGC TCTAGACCCC CCGCTGTTTA CCCGCTTCCT TCGGATTCAC 
CCGCGGAGCT GGGCGCACCA TATCGCCCTG AGGCTGGAGC TTTTGGGCTG CGAGGCACAG 
CAGCAGTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005227696.1
CDS	152..7123
Translation	MHIKLSTCFFLCLWPCSFSAIRRYYLGAVELSWDYMQSELLSELHVNTRFPPRVPKPFPFNTSVMYRKTVFVEFTDQLFNIAKPRPPWMGLLGPTIQAEVYDTVVITFKNMASHPVSLHAIGVSYWKSSEGAAYEDETSQREKEDDKVIPGKSHTYVWQILKENGPTASDPPCLTYSYLSHVDLVKDVNSGLIGALLICREGTLIKERTQTLHKFVLLFAVFDEGKSWHSGKNESLTRVMDSASALHTINGYINRSLPGLIGCHKKSVYWHVIGMGTTPEVHSIFLEGHTFLVRNHRQASLEISPITFLTAQTVLMDCGQFLLFCRISSHQHDGMEAYVKVDSCPEEPRLWMKNNQEEDYDDGLDDSDMDVVRFDGDSAPPFIQMRSVAKKHPKTWVHYIAAEEDDWDYAPSVLTTSDRSYKSLYLNHGPQRIGRKYRKVRFIAYTDGTFKTREAIQHESGILGPLLYGEVGDTLLIIFKNQASRPYNIYPHGITDVSPLHSGRFPKGVKHLKDMPILPGEVFKYKWTVTVEDGPTKSDPRCLTRYYSSFINLEKDLASGLIGPLLICYKESVDQRGNQMMSDKRNVILFSVFDENKSWYLTENIQRFLPNGVQPQDPEFQVSNVMHSINGYVFDSLQLSVCLHEVAYWYILSVGAQIDFLSVFFSGYTFKHKMVYEDTLTLFPFSGETVFMSMENPGLWVLGCHNSDFRNRGMTALLKVDSCDRNVGDYYDTYEDIPTLLSDDNVIEPRSFSKNSRYPSTRQKQFKPTMTPENDMEKIDPQSGEKMQLLTEQSVSSSDLLMLLGQNPTPHRLSLSDLQETRNEATDHLPGPTERIKGPSPSEVAHLRPELHHSGERVFTQEPELPVRLNENLGTTIMAELNKLNLKISSSSNNLMPLPTIPSDKLSAGTKKTGSFGPPNMPVNFNSQLGAIVFGKNLSHVIGSRVPLGLSKEDNNSKLLEAALMNSQESSLGENALSLESDRLFKEERVHGPVSLNKDDALFKVNISLVKTDKAPANSTTNRKTHIDDPTVLIENSTSVWQDIVIDSNTNFQEVTSLIHDELFMHKNTTSFGLSHMSNKTTSSKKREMIHQEKEGPKPLDTKYPDTPFFKTLFLPDSTNVIKRTHDKNSLSSGQRPSPKQLTSSVSENSVKDQNVLSEKNKAVAGEDEFSNNTSLKEMIFPNTKSIVLTNLANVQENDTDNQGKKSQEETEKKETLIPENTVLPQVYTVIGTKNFLKNLFSLSTKQNVRGLDEETYTPGLQDNRSLSDSAKRARIHAAHFSKGRVEANVEGLGNQTKQMVEKSPSTTKLSPIPSQQDAVPHRDKRGLKQSRLPREEVKLERGVTLNDTSTQWSKSMKHLTQGTFTQIEHNKMEKRTITQSFLPDCSLRSHRSIRPTDVTKIPPQHNSSHPPASTYGYNLGASHSGVQETSHFLHGAKKNNLSLAFLTLEVIGSQRKSSTPGKQATNPLMYKKLENMFLLKPGLSEASGKVVHQEDLPTKTSKVSPAHLDLREEIFLQKTQGLVKLNKVYRTGKVPSLKWTTESSEEIPSKLLGLLAWDNQSATLIPKEDWKSLERSHKLTSLKTKDITLPLDPSENYYSIAVTNEGEDKAQREAPWPKQGGTGRVCCQNPPILKRHQREITLTTFQPEPDKTDYDDTLSIETKREDFDIYGEDENQDPRSFQKRTRHYFIAAVERLWDYGMSRSPYALRNRSQNGGVPQFKKVVFEEFTDGSFTQAIYRGQLNEHLGLLGPYIRAEVEDNIMVTFKNQASRPYSFYSSLISYNGDQRQGAEPRKKFVKPNETQSYFWKVQHHMAPTKDEFDCKAWAYFSDVDLEKDLHSGLIGPILICRVDTLSAAHGRQVTIQEFALFFTIFDETKSWYFAENMARNCGAPCHVQPEDPTFQEKYRFHAINGYVMDTLPGLVMAQHQRIRWYLLSMGSNENIHSIHFSGHVFTVRKTEEYKMAVYNLYPGVFETVEMLPSKVGTWRIECLIGEHLQAGMSTLFLVYSKECQIPLGMASGRIRDFQITASGQYGQWAPKLARLHYSGSINAWSTKDPSPWIKVDLLAPMIIHSILTQGARQKFSSLYISQFIIMYSLDGQRWQGYRGNSTGTLMVFFGNVDSSGVKHNIFNPPIIARYIRLHPTHYSIRSTLRMELMGCDLNSCNMPLGMENKAISDTQITASSHLRNMFATWSPSQARLNLQGRTNAWRPQVNNPKEWLQVDFQKTMRVTGITTQGVKSLLTSMYVKEFLISSSQDGHNWTPFLQNGKVKVFQGNQDSFTPVVNALDPPLFTRFLRIHPRSWAHHIALRLELLGCEAQQQY

Target ORF information:

RefSeq Version	XM_005227639.3
Organism	Bos taurus(cattle)
Definition	Bos taurus coagulation factor VIII (F8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005227639.3

ORF Insert Sequence:

ATGCACATCA AGCTCTCTAC CTGCTTCTTT CTGTGCCTCT GGCCATGCAG CTTCAGTGCC 
ATCAGAAGAT ACTACCTGGG TGCAGTGGAA CTCTCCTGGG ACTATATGCA AAGTGAACTG 
CTCAGTGAGC TGCATGTGAA CACAAGGTTT CCTCCCAGAG TGCCCAAACC TTTTCCATTC 
AACACCTCAG TCATGTACAG AAAGACTGTG TTTGTAGAGT TCACGGATCA ACTTTTCAAC 
ATCGCCAAGC CCAGGCCACC ATGGATGGGT CTGCTGGGTC CAACTATCCA GGCTGAGGTT 
TATGACACCG TGGTCATTAC ATTTAAGAAT ATGGCTTCTC ATCCTGTTAG TCTTCATGCT 
ATTGGCGTAT CCTACTGGAA ATCTTCTGAA GGTGCTGCAT ATGAGGATGA AACCAGCCAA 
AGGGAGAAGG AAGATGACAA AGTCATTCCT GGTAAAAGTC ATACCTACGT CTGGCAGATC 
CTGAAAGAAA ATGGTCCAAC AGCCTCTGAC CCACCATGTC TCACCTACTC ATATCTTTCT 
CATGTGGACC TGGTGAAAGA CGTGAACTCA GGTCTCATTG GAGCCCTGCT AATTTGTAGG 
GAAGGAACTC TGATCAAAGA AAGGACACAG ACCTTGCACA AATTCGTACT ACTGTTTGCT 
GTATTTGATG AAGGGAAAAG TTGGCACTCG GGAAAAAATG AGTCCTTGAC ACGTGTTATG 
GATTCTGCAT CTGCACTGCA CACCATCAAT GGCTATATAA ACAGGTCTCT GCCAGGTCTG 
ATTGGATGTC ATAAGAAATC AGTCTATTGG CACGTGATTG GAATGGGCAC CACCCCGGAA 
GTGCACTCAA TTTTCCTTGA AGGCCACACA TTTCTCGTGA GGAACCATCG CCAGGCTTCC 
TTGGAGATCT CACCAATAAC TTTCCTTACC GCTCAGACAG TCCTGATGGA CTGTGGCCAG 
TTTCTACTGT TTTGTCGTAT CTCTTCCCAC CAACATGATG GTATGGAAGC TTATGTCAAA 
GTAGATAGTT GCCCAGAGGA ACCCCGACTA TGGATGAAAA ATAACCAAGA AGAAGATTAT 
GATGATGGTT TGGATGACTC TGACATGGAT GTGGTCAGGT TTGATGGCGA CAGTGCGCCT 
CCCTTTATCC AAATGCGCTC AGTTGCAAAG AAGCATCCTA AAACCTGGGT CCACTACATT 
GCTGCTGAAG AGGATGACTG GGACTATGCC CCCTCGGTCC TCACCACCAG TGACAGAAGT 
TATAAAAGTC TGTATCTGAA CCACGGTCCT CAGCGGATTG GTAGGAAGTA CAGAAAAGTA 
CGGTTTATAG CTTACACAGA TGGAACATTT AAGACTCGTG AAGCTATTCA GCATGAATCA 
GGGATCCTGG GGCCTTTACT TTATGGAGAA GTTGGAGACA CACTTTTGAT TATATTTAAG 
AATCAAGCAA GCCGGCCATA TAACATCTAC CCTCATGGAA TCACTGATGT CAGTCCTTTG 
CACTCAGGGA GATTTCCAAA AGGTGTGAAA CATTTGAAAG ACATGCCAAT TCTGCCAGGA 
GAAGTGTTCA AGTATAAATG GACAGTGACT GTAGAAGATG GGCCAACTAA ATCAGATCCT 
CGGTGTCTGA CCCGATATTA CTCGAGTTTC ATTAACTTAG AGAAAGATCT AGCTTCAGGA 
CTCATTGGCC CCCTCCTCAT CTGCTACAAA GAATCTGTAG ATCAAAGAGG AAACCAGATG 
ATGTCAGACA AGAGAAATGT CATCCTGTTT TCTGTATTTG ATGAGAATAA AAGTTGGTAC 
CTCACAGAGA ATATTCAGCG CTTCCTCCCC AATGGAGTAC AGCCCCAGGA TCCAGAGTTC 
CAAGTCTCCA ATGTCATGCA TAGCATCAAT GGCTATGTTT TTGATAGCTT GCAGCTGTCG 
GTTTGTTTGC ATGAGGTGGC ATACTGGTAC ATTCTAAGTG TTGGAGCCCA AATTGACTTC 
CTCTCTGTCT TCTTCTCTGG ATATACCTTC AAACACAAAA TGGTCTATGA AGATACACTC 
ACCCTATTCC CCTTTTCAGG AGAAACTGTC TTCATGTCAA TGGAAAATCC AGGTCTGTGG 
GTTCTGGGGT GCCACAACTC AGACTTTCGA AACAGAGGCA TGACAGCCTT ACTGAAGGTT 
GATAGTTGTG ACAGGAACGT TGGCGATTAT TATGACACAT ATGAAGATAT TCCAACCTTG 
CTGAGTGACG ACAATGTCAT TGAACCCAGA AGCTTCTCCA AGAATTCAAG GTACCCTAGC 
ACTAGGCAAA AGCAATTCAA ACCCACCATG ACTCCAGAAA ATGACATGGA GAAGATTGAC 
CCTCAGTCTG GAGAGAAAAT GCAGCTGCTT ACAGAACAAA GTGTCTCCTC TAGTGATCTG 
TTGATGCTCT TGGGACAGAA TCCTACTCCA CACAGATTAT CCTTATCTGA TCTCCAAGAA 
ACCAGAAATG AGGCTACTGA TCATTTACCT GGACCAACAG AAAGAATCAA GGGCCCATCC 
CCATCTGAAG TGGCACATCT TAGACCAGAG CTGCATCACA GTGGGGAAAG AGTATTTACT 
CAAGAGCCAG AACTGCCAGT AAGATTAAAT GAGAATTTGG GAACAACTAT AATGGCAGAG 
TTGAACAAAC TTAATTTAAA AATTTCAAGT TCATCAAACA ATCTAATGCC TTTACCAACA 
ATTCCATCAG ACAAGTTGTC AGCAGGTACT AAAAAGACAG GTTCCTTCGG ACCCCCAAAT 
ATGCCAGTTA ATTTTAATAG TCAATTAGGT GCCATTGTAT TTGGCAAAAA TTTATCTCAT 
GTTATTGGAT CACGTGTACC TTTGGGCTTG AGTAAAGAAG ATAATAATTC CAAGTTGTTA 
GAAGCAGCTT TAATGAATAG TCAAGAAAGT TCACTGGGAG AAAATGCATT ATCACTGGAG 
AGTGATAGGC TATTTAAAGA GGAAAGAGTT CATGGACCTG TGTCATTGAA CAAAGATGAT 
GCTTTATTTA AAGTGAATAT CTCTTTGGTA AAGACAGACA AGGCACCAGC TAACTCAACA 
ACTAATAGAA AGACTCACAT TGATGACCCA ACAGTATTAA TTGAGAATAG TACATCAGTC 
TGGCAGGATA TTGTAATAGA CAGTAATACC AACTTTCAAG AAGTGACTTC TTTGATTCAT 
GATGAATTGT TTATGCACAA AAATACTACA TCTTTTGGAC TAAGCCATAT GTCAAATAAA 
ACTACTTCAT CCAAAAAAAG GGAAATGATC CACCAAGAAA AAGAAGGCCC TAAGCCACTA 
GATACAAAAT ATCCAGATAC ACCATTCTTC AAGACTCTGT TTTTGCCAGA TTCAACAAAC 
GTGATAAAAA GGACCCACGA CAAGAACTCC CTAAGCTCTG GGCAAAGGCC CAGTCCAAAG 
CAATTAACAT CATCAGTATC AGAAAACTCT GTGAAAGATC AGAATGTCTT ATCAGAAAAG 
AATAAGGCAG TAGCAGGAGA GGATGAATTT AGCAACAACA CCAGCCTCAA AGAGATGATT 
TTTCCAAATA CCAAAAGCAT AGTTCTTACT AACTTGGCTA ATGTCCAAGA AAATGATACA 
GACAATCAAG GAAAAAAATC CCAGGAAGAA ACAGAAAAGA AAGAGACATT AATCCCTGAG 
AACACAGTTT TGCCTCAGGT GTATACAGTG ATTGGAACTA AGAATTTCCT GAAGAATCTC 
TTTTCACTGA GCACTAAGCA AAATGTCAGA GGTTTAGATG AAGAGACATA TACTCCAGGA 
CTTCAAGACA ACAGGTCATT AAGTGATTCG GCAAAAAGAG CCAGGATTCA TGCAGCCCAT 
TTCTCAAAAG GAAGGGTGGA AGCAAACGTG GAAGGCTTAG GGAATCAAAC AAAACAAATG 
GTAGAGAAAT CTCCAAGCAC TACAAAACTG TCTCCTATCC CAAGTCAGCA GGATGCAGTT 
CCTCACCGTG ATAAGCGGGG TTTGAAACAA TCCAGACTCC CACGAGAAGA AGTAAAGCTT 
GAAAGGGGGG TAACTCTGAA TGACACCTCA ACTCAGTGGT CCAAAAGCAT GAAACATTTG 
ACCCAGGGTA CCTTCACACA GATAGAACAC AACAAGATGG AGAAAAGGAC CATTACTCAG 
TCCTTCTTAC CAGATTGTTC TTTGAGGAGT CATAGGTCAA TTAGACCTAC AGATGTGACC 
AAGATCCCAC CTCAACATAA CTCTTCTCAT CCTCCAGCAT CAACCTATGG TTATAACTTG 
GGGGCAAGTC ATTCTGGGGT CCAAGAAACC AGTCATTTCT TACACGGAGC CAAGAAAAAT 
AATCTTTCTT TAGCCTTCCT AACATTGGAA GTGATCGGAA GTCAAAGAAA GAGCAGCACC 
CCGGGGAAAC AGGCCACAAA TCCACTCATG TACAAAAAAC TTGAGAACAT GTTTCTGTTG 
AAACCAGGCT TGTCTGAAGC ATCTGGGAAA GTTGTTCATC AGGAAGACTT GCCTACAAAA 
ACTAGCAAGG TTTCTCCTGC CCACCTGGAT CTCAGGGAAG AGATCTTCCT TCAGAAAACA 
CAGGGACTTG TTAAATTGAA TAAAGTCTAC AGGACTGGAA AAGTGCCATC TCTGAAATGG 
ACAACAGAAA GCTCTGAAGA GATTCCCTCC AAGCTGCTGG GTCTCCTTGC TTGGGATAAC 
CAGTCAGCTA CCTTGATACC AAAAGAAGAC TGGAAATCCC TAGAGAGGTC ACACAAACTC 
ACGTCTTTGA AGACAAAAGA TATCACTTTG CCCCTAGACC CTAGTGAAAA CTATTATTCA 
ATAGCAGTAA CAAATGAAGG AGAAGATAAG GCCCAAAGAG AAGCTCCCTG GCCAAAGCAA 
GGAGGGACTG GAAGGGTGTG CTGTCAAAAT CCACCAATCT TGAAACGCCA TCAAAGGGAG 
ATAACCCTTA CTACTTTTCA GCCAGAGCCA GACAAAACTG ACTATGATGA TACTTTGTCA 
ATTGAAACAA AGAGAGAAGA TTTTGACATT TATGGTGAAG ATGAAAATCA GGACCCCCGC 
AGCTTTCAAA AGAGAACACG CCACTATTTT ATTGCTGCAG TGGAGCGGCT CTGGGATTAT 
GGGATGAGTA GATCCCCCTA CGCACTAAGA AACAGGTCTC AGAATGGAGG AGTCCCTCAG 
TTCAAAAAGG TGGTATTCGA GGAATTTACT GATGGCTCCT TTACTCAGGC CATATACCGT 
GGACAACTAA ATGAACACCT GGGACTCTTG GGACCATATA TAAGAGCAGA AGTGGAAGAC 
AATATCATGG TAACTTTCAA AAACCAGGCC TCTCGTCCCT ACTCCTTCTA TTCTAGCCTT 
ATTTCTTATA ACGGAGATCA GAGACAAGGA GCAGAACCTC GAAAAAAGTT TGTCAAGCCT 
AATGAAACCC AAAGCTACTT TTGGAAAGTG CAGCACCATA TGGCACCCAC CAAAGATGAG 
TTTGACTGCA AAGCCTGGGC TTACTTTTCT GACGTTGATC TGGAAAAAGA TTTGCACTCA 
GGCTTGATTG GCCCCATTCT GATCTGCCGC GTGGACACGC TGAGTGCTGC TCATGGGAGA 
CAAGTAACAA TACAGGAATT CGCTCTGTTT TTCACCATTT TTGATGAAAC CAAGAGCTGG 
TACTTTGCCG AAAACATGGC AAGGAACTGC GGGGCGCCCT GCCATGTCCA GCCAGAGGAC 
CCTACTTTTC AAGAAAAGTA TCGCTTCCAT GCAATCAATG GCTACGTGAT GGATACACTC 
CCTGGCTTAG TCATGGCTCA GCATCAAAGG ATTAGGTGGT ATCTGCTCAG CATGGGCAGC 
AATGAAAATA TCCATTCCAT TCATTTCAGT GGCCATGTGT TCACTGTGCG AAAAACGGAG 
GAGTATAAAA TGGCGGTCTA CAATCTCTAC CCAGGTGTCT TTGAGACGGT GGAAATGCTA 
CCATCCAAGG TTGGGACTTG GCGGATAGAA TGTCTTATTG GCGAGCACCT ACAAGCTGGG 
ATGAGCACTC TCTTCCTGGT GTACAGCAAG GAGTGTCAAA TTCCACTGGG AATGGCTTCT 
GGACGCATTA GAGATTTTCA GATTACAGCT TCAGGACAAT ATGGACAGTG GGCCCCCAAG 
CTGGCCAGAC TTCATTATTC TGGATCAATC AACGCCTGGA GCACCAAGGA TCCCTCTCCT 
TGGATCAAGG TGGATCTGTT GGCGCCGATG ATTATTCACA GCATCCTGAC TCAGGGTGCC 
CGGCAGAAGT TCTCCAGCCT GTACATCTCT CAGTTTATCA TCATGTACAG CCTCGATGGA 
CAGCGGTGGC AGGGTTATCG GGGGAACTCC ACTGGGACCT TAATGGTGTT CTTTGGTAAT 
GTGGATTCAT CTGGAGTAAA ACACAATATT TTTAACCCTC CAATTATTGC TAGATATATC 
CGTTTACACC CAACTCATTA CAGCATCCGC AGCACTCTTC GCATGGAGTT GATGGGCTGT 
GATTTAAACA GTTGCAACAT GCCACTGGGA ATGGAGAATA AAGCTATATC AGATACACAG 
ATTACTGCCT CATCCCACTT ACGCAACATG TTTGCCACCT GGTCTCCTTC ACAAGCCCGA 
CTTAACCTTC AAGGGAGGAC AAATGCCTGG AGGCCCCAGG TGAATAATCC AAAAGAGTGG 
CTACAAGTGG ACTTCCAGAA GACAATGAGA GTTACAGGAA TAACTACCCA AGGGGTGAAA 
TCTCTGCTTA CCAGCATGTA TGTGAAGGAG TTCCTCATAT CCAGTAGTCA AGATGGCCAT 
AACTGGACTC CATTTCTTCA GAATGGCAAA GTGAAGGTTT TTCAGGGAAA TCAAGACTCC 
TTCACCCCCG TGGTGAATGC TCTAGACCCC CCGCTGTTTA CCCGCTTCCT TCGGATTCAC 
CCGCGGAGCT GGGCGCACCA TATCGCCCTG AGGCTGGAGC TTTTGGGCTG CGAGGCACAG 
CAGCAGTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

F8 ( XM_005227639.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005227696.1 Bos taurus coagulation factor VIII (F8), transcript variant X1, mRNA.

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CloneID	OBa35243
Clone ID Related Accession (Same CDS sequence)	XM_005227639.3 , XM_005227639.4 , XM_024987717.1
Accession Version	XM_005227639.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	coagulation factor VIII isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005227639.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACATCA AGCTCTCTAC CTGCTTCTTT CTGTGCCTCT GGCCATGCAG CTTCAGTGCC 
ATCAGAAGAT ACTACCTGGG TGCAGTGGAA CTCTCCTGGG ACTATATGCA AAGTGAACTG 
CTCAGTGAGC TGCATGTGAA CACAAGGTTT CCTCCCAGAG TGCCCAAACC TTTTCCATTC 
AACACCTCAG TCATGTACAG AAAGACTGTG TTTGTAGAGT TCACGGATCA ACTTTTCAAC 
ATCGCCAAGC CCAGGCCACC ATGGATGGGT CTGCTGGGTC CAACTATCCA GGCTGAGGTT 
TATGACACCG TGGTCATTAC ATTTAAGAAT ATGGCTTCTC ATCCTGTTAG TCTTCATGCT 
ATTGGCGTAT CCTACTGGAA ATCTTCTGAA GGTGCTGCAT ATGAGGATGA AACCAGCCAA 
AGGGAGAAGG AAGATGACAA AGTCATTCCT GGTAAAAGTC ATACCTACGT CTGGCAGATC 
CTGAAAGAAA ATGGTCCAAC AGCCTCTGAC CCACCATGTC TCACCTACTC ATATCTTTCT 
CATGTGGACC TGGTGAAAGA CGTGAACTCA GGTCTCATTG GAGCCCTGCT AATTTGTAGG 
GAAGGAACTC TGATCAAAGA AAGGACACAG ACCTTGCACA AATTCGTACT ACTGTTTGCT 
GTATTTGATG AAGGGAAAAG TTGGCACTCG GGAAAAAATG AGTCCTTGAC ACGTGTTATG 
GATTCTGCAT CTGCACTGCA CACCATCAAT GGCTATATAA ACAGGTCTCT GCCAGGTCTG 
ATTGGATGTC ATAAGAAATC AGTCTATTGG CACGTGATTG GAATGGGCAC CACCCCGGAA 
GTGCACTCAA TTTTCCTTGA AGGCCACACA TTTCTCGTGA GGAACCATCG CCAGGCTTCC 
TTGGAGATCT CACCAATAAC TTTCCTTACC GCTCAGACAG TCCTGATGGA CTGTGGCCAG 
TTTCTACTGT TTTGTCGTAT CTCTTCCCAC CAACATGATG GTATGGAAGC TTATGTCAAA 
GTAGATAGTT GCCCAGAGGA ACCCCGACTA TGGATGAAAA ATAACCAAGA AGAAGATTAT 
GATGATGGTT TGGATGACTC TGACATGGAT GTGGTCAGGT TTGATGGCGA CAGTGCGCCT 
CCCTTTATCC AAATGCGCTC AGTTGCAAAG AAGCATCCTA AAACCTGGGT CCACTACATT 
GCTGCTGAAG AGGATGACTG GGACTATGCC CCCTCGGTCC TCACCACCAG TGACAGAAGT 
TATAAAAGTC TGTATCTGAA CCACGGTCCT CAGCGGATTG GTAGGAAGTA CAGAAAAGTA 
CGGTTTATAG CTTACACAGA TGGAACATTT AAGACTCGTG AAGCTATTCA GCATGAATCA 
GGGATCCTGG GGCCTTTACT TTATGGAGAA GTTGGAGACA CACTTTTGAT TATATTTAAG 
AATCAAGCAA GCCGGCCATA TAACATCTAC CCTCATGGAA TCACTGATGT CAGTCCTTTG 
CACTCAGGGA GATTTCCAAA AGGTGTGAAA CATTTGAAAG ACATGCCAAT TCTGCCAGGA 
GAAGTGTTCA AGTATAAATG GACAGTGACT GTAGAAGATG GGCCAACTAA ATCAGATCCT 
CGGTGTCTGA CCCGATATTA CTCGAGTTTC ATTAACTTAG AGAAAGATCT AGCTTCAGGA 
CTCATTGGCC CCCTCCTCAT CTGCTACAAA GAATCTGTAG ATCAAAGAGG AAACCAGATG 
ATGTCAGACA AGAGAAATGT CATCCTGTTT TCTGTATTTG ATGAGAATAA AAGTTGGTAC 
CTCACAGAGA ATATTCAGCG CTTCCTCCCC AATGGAGTAC AGCCCCAGGA TCCAGAGTTC 
CAAGTCTCCA ATGTCATGCA TAGCATCAAT GGCTATGTTT TTGATAGCTT GCAGCTGTCG 
GTTTGTTTGC ATGAGGTGGC ATACTGGTAC ATTCTAAGTG TTGGAGCCCA AATTGACTTC 
CTCTCTGTCT TCTTCTCTGG ATATACCTTC AAACACAAAA TGGTCTATGA AGATACACTC 
ACCCTATTCC CCTTTTCAGG AGAAACTGTC TTCATGTCAA TGGAAAATCC AGGTCTGTGG 
GTTCTGGGGT GCCACAACTC AGACTTTCGA AACAGAGGCA TGACAGCCTT ACTGAAGGTT 
GATAGTTGTG ACAGGAACGT TGGCGATTAT TATGACACAT ATGAAGATAT TCCAACCTTG 
CTGAGTGACG ACAATGTCAT TGAACCCAGA AGCTTCTCCA AGAATTCAAG GTACCCTAGC 
ACTAGGCAAA AGCAATTCAA ACCCACCATG ACTCCAGAAA ATGACATGGA GAAGATTGAC 
CCTCAGTCTG GAGAGAAAAT GCAGCTGCTT ACAGAACAAA GTGTCTCCTC TAGTGATCTG 
TTGATGCTCT TGGGACAGAA TCCTACTCCA CACAGATTAT CCTTATCTGA TCTCCAAGAA 
ACCAGAAATG AGGCTACTGA TCATTTACCT GGACCAACAG AAAGAATCAA GGGCCCATCC 
CCATCTGAAG TGGCACATCT TAGACCAGAG CTGCATCACA GTGGGGAAAG AGTATTTACT 
CAAGAGCCAG AACTGCCAGT AAGATTAAAT GAGAATTTGG GAACAACTAT AATGGCAGAG 
TTGAACAAAC TTAATTTAAA AATTTCAAGT TCATCAAACA ATCTAATGCC TTTACCAACA 
ATTCCATCAG ACAAGTTGTC AGCAGGTACT AAAAAGACAG GTTCCTTCGG ACCCCCAAAT 
ATGCCAGTTA ATTTTAATAG TCAATTAGGT GCCATTGTAT TTGGCAAAAA TTTATCTCAT 
GTTATTGGAT CACGTGTACC TTTGGGCTTG AGTAAAGAAG ATAATAATTC CAAGTTGTTA 
GAAGCAGCTT TAATGAATAG TCAAGAAAGT TCACTGGGAG AAAATGCATT ATCACTGGAG 
AGTGATAGGC TATTTAAAGA GGAAAGAGTT CATGGACCTG TGTCATTGAA CAAAGATGAT 
GCTTTATTTA AAGTGAATAT CTCTTTGGTA AAGACAGACA AGGCACCAGC TAACTCAACA 
ACTAATAGAA AGACTCACAT TGATGACCCA ACAGTATTAA TTGAGAATAG TACATCAGTC 
TGGCAGGATA TTGTAATAGA CAGTAATACC AACTTTCAAG AAGTGACTTC TTTGATTCAT 
GATGAATTGT TTATGCACAA AAATACTACA TCTTTTGGAC TAAGCCATAT GTCAAATAAA 
ACTACTTCAT CCAAAAAAAG GGAAATGATC CACCAAGAAA AAGAAGGCCC TAAGCCACTA 
GATACAAAAT ATCCAGATAC ACCATTCTTC AAGACTCTGT TTTTGCCAGA TTCAACAAAC 
GTGATAAAAA GGACCCACGA CAAGAACTCC CTAAGCTCTG GGCAAAGGCC CAGTCCAAAG 
CAATTAACAT CATCAGTATC AGAAAACTCT GTGAAAGATC AGAATGTCTT ATCAGAAAAG 
AATAAGGCAG TAGCAGGAGA GGATGAATTT AGCAACAACA CCAGCCTCAA AGAGATGATT 
TTTCCAAATA CCAAAAGCAT AGTTCTTACT AACTTGGCTA ATGTCCAAGA AAATGATACA 
GACAATCAAG GAAAAAAATC CCAGGAAGAA ACAGAAAAGA AAGAGACATT AATCCCTGAG 
AACACAGTTT TGCCTCAGGT GTATACAGTG ATTGGAACTA AGAATTTCCT GAAGAATCTC 
TTTTCACTGA GCACTAAGCA AAATGTCAGA GGTTTAGATG AAGAGACATA TACTCCAGGA 
CTTCAAGACA ACAGGTCATT AAGTGATTCG GCAAAAAGAG CCAGGATTCA TGCAGCCCAT 
TTCTCAAAAG GAAGGGTGGA AGCAAACGTG GAAGGCTTAG GGAATCAAAC AAAACAAATG 
GTAGAGAAAT CTCCAAGCAC TACAAAACTG TCTCCTATCC CAAGTCAGCA GGATGCAGTT 
CCTCACCGTG ATAAGCGGGG TTTGAAACAA TCCAGACTCC CACGAGAAGA AGTAAAGCTT 
GAAAGGGGGG TAACTCTGAA TGACACCTCA ACTCAGTGGT CCAAAAGCAT GAAACATTTG 
ACCCAGGGTA CCTTCACACA GATAGAACAC AACAAGATGG AGAAAAGGAC CATTACTCAG 
TCCTTCTTAC CAGATTGTTC TTTGAGGAGT CATAGGTCAA TTAGACCTAC AGATGTGACC 
AAGATCCCAC CTCAACATAA CTCTTCTCAT CCTCCAGCAT CAACCTATGG TTATAACTTG 
GGGGCAAGTC ATTCTGGGGT CCAAGAAACC AGTCATTTCT TACACGGAGC CAAGAAAAAT 
AATCTTTCTT TAGCCTTCCT AACATTGGAA GTGATCGGAA GTCAAAGAAA GAGCAGCACC 
CCGGGGAAAC AGGCCACAAA TCCACTCATG TACAAAAAAC TTGAGAACAT GTTTCTGTTG 
AAACCAGGCT TGTCTGAAGC ATCTGGGAAA GTTGTTCATC AGGAAGACTT GCCTACAAAA 
ACTAGCAAGG TTTCTCCTGC CCACCTGGAT CTCAGGGAAG AGATCTTCCT TCAGAAAACA 
CAGGGACTTG TTAAATTGAA TAAAGTCTAC AGGACTGGAA AAGTGCCATC TCTGAAATGG 
ACAACAGAAA GCTCTGAAGA GATTCCCTCC AAGCTGCTGG GTCTCCTTGC TTGGGATAAC 
CAGTCAGCTA CCTTGATACC AAAAGAAGAC TGGAAATCCC TAGAGAGGTC ACACAAACTC 
ACGTCTTTGA AGACAAAAGA TATCACTTTG CCCCTAGACC CTAGTGAAAA CTATTATTCA 
ATAGCAGTAA CAAATGAAGG AGAAGATAAG GCCCAAAGAG AAGCTCCCTG GCCAAAGCAA 
GGAGGGACTG GAAGGGTGTG CTGTCAAAAT CCACCAATCT TGAAACGCCA TCAAAGGGAG 
ATAACCCTTA CTACTTTTCA GCCAGAGCCA GACAAAACTG ACTATGATGA TACTTTGTCA 
ATTGAAACAA AGAGAGAAGA TTTTGACATT TATGGTGAAG ATGAAAATCA GGACCCCCGC 
AGCTTTCAAA AGAGAACACG CCACTATTTT ATTGCTGCAG TGGAGCGGCT CTGGGATTAT 
GGGATGAGTA GATCCCCCTA CGCACTAAGA AACAGGTCTC AGAATGGAGG AGTCCCTCAG 
TTCAAAAAGG TGGTATTCGA GGAATTTACT GATGGCTCCT TTACTCAGGC CATATACCGT 
GGACAACTAA ATGAACACCT GGGACTCTTG GGACCATATA TAAGAGCAGA AGTGGAAGAC 
AATATCATGG TAACTTTCAA AAACCAGGCC TCTCGTCCCT ACTCCTTCTA TTCTAGCCTT 
ATTTCTTATA ACGGAGATCA GAGACAAGGA GCAGAACCTC GAAAAAAGTT TGTCAAGCCT 
AATGAAACCC AAAGCTACTT TTGGAAAGTG CAGCACCATA TGGCACCCAC CAAAGATGAG 
TTTGACTGCA AAGCCTGGGC TTACTTTTCT GACGTTGATC TGGAAAAAGA TTTGCACTCA 
GGCTTGATTG GCCCCATTCT GATCTGCCGC GTGGACACGC TGAGTGCTGC TCATGGGAGA 
CAAGTAACAA TACAGGAATT CGCTCTGTTT TTCACCATTT TTGATGAAAC CAAGAGCTGG 
TACTTTGCCG AAAACATGGC AAGGAACTGC GGGGCGCCCT GCCATGTCCA GCCAGAGGAC 
CCTACTTTTC AAGAAAAGTA TCGCTTCCAT GCAATCAATG GCTACGTGAT GGATACACTC 
CCTGGCTTAG TCATGGCTCA GCATCAAAGG ATTAGGTGGT ATCTGCTCAG CATGGGCAGC 
AATGAAAATA TCCATTCCAT TCATTTCAGT GGCCATGTGT TCACTGTGCG AAAAACGGAG 
GAGTATAAAA TGGCGGTCTA CAATCTCTAC CCAGGTGTCT TTGAGACGGT GGAAATGCTA 
CCATCCAAGG TTGGGACTTG GCGGATAGAA TGTCTTATTG GCGAGCACCT ACAAGCTGGG 
ATGAGCACTC TCTTCCTGGT GTACAGCAAG GAGTGTCAAA TTCCACTGGG AATGGCTTCT 
GGACGCATTA GAGATTTTCA GATTACAGCT TCAGGACAAT ATGGACAGTG GGCCCCCAAG 
CTGGCCAGAC TTCATTATTC TGGATCAATC AACGCCTGGA GCACCAAGGA TCCCTCTCCT 
TGGATCAAGG TGGATCTGTT GGCGCCGATG ATTATTCACA GCATCCTGAC TCAGGGTGCC 
CGGCAGAAGT TCTCCAGCCT GTACATCTCT CAGTTTATCA TCATGTACAG CCTCGATGGA 
CAGCGGTGGC AGGGTTATCG GGGGAACTCC ACTGGGACCT TAATGGTGTT CTTTGGTAAT 
GTGGATTCAT CTGGAGTAAA ACACAATATT TTTAACCCTC CAATTATTGC TAGATATATC 
CGTTTACACC CAACTCATTA CAGCATCCGC AGCACTCTTC GCATGGAGTT GATGGGCTGT 
GATTTAAACA GTTGCAACAT GCCACTGGGA ATGGAGAATA AAGCTATATC AGATACACAG 
ATTACTGCCT CATCCCACTT ACGCAACATG TTTGCCACCT GGTCTCCTTC ACAAGCCCGA 
CTTAACCTTC AAGGGAGGAC AAATGCCTGG AGGCCCCAGG TGAATAATCC AAAAGAGTGG 
CTACAAGTGG ACTTCCAGAA GACAATGAGA GTTACAGGAA TAACTACCCA AGGGGTGAAA 
TCTCTGCTTA CCAGCATGTA TGTGAAGGAG TTCCTCATAT CCAGTAGTCA AGATGGCCAT 
AACTGGACTC CATTTCTTCA GAATGGCAAA GTGAAGGTTT TTCAGGGAAA TCAAGACTCC 
TTCACCCCCG TGGTGAATGC TCTAGACCCC CCGCTGTTTA CCCGCTTCCT TCGGATTCAC 
CCGCGGAGCT GGGCGCACCA TATCGCCCTG AGGCTGGAGC TTTTGGGCTG CGAGGCACAG 
CAGCAGTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005227696.1
CDS	194..7165
Translation	MHIKLSTCFFLCLWPCSFSAIRRYYLGAVELSWDYMQSELLSELHVNTRFPPRVPKPFPFNTSVMYRKTVFVEFTDQLFNIAKPRPPWMGLLGPTIQAEVYDTVVITFKNMASHPVSLHAIGVSYWKSSEGAAYEDETSQREKEDDKVIPGKSHTYVWQILKENGPTASDPPCLTYSYLSHVDLVKDVNSGLIGALLICREGTLIKERTQTLHKFVLLFAVFDEGKSWHSGKNESLTRVMDSASALHTINGYINRSLPGLIGCHKKSVYWHVIGMGTTPEVHSIFLEGHTFLVRNHRQASLEISPITFLTAQTVLMDCGQFLLFCRISSHQHDGMEAYVKVDSCPEEPRLWMKNNQEEDYDDGLDDSDMDVVRFDGDSAPPFIQMRSVAKKHPKTWVHYIAAEEDDWDYAPSVLTTSDRSYKSLYLNHGPQRIGRKYRKVRFIAYTDGTFKTREAIQHESGILGPLLYGEVGDTLLIIFKNQASRPYNIYPHGITDVSPLHSGRFPKGVKHLKDMPILPGEVFKYKWTVTVEDGPTKSDPRCLTRYYSSFINLEKDLASGLIGPLLICYKESVDQRGNQMMSDKRNVILFSVFDENKSWYLTENIQRFLPNGVQPQDPEFQVSNVMHSINGYVFDSLQLSVCLHEVAYWYILSVGAQIDFLSVFFSGYTFKHKMVYEDTLTLFPFSGETVFMSMENPGLWVLGCHNSDFRNRGMTALLKVDSCDRNVGDYYDTYEDIPTLLSDDNVIEPRSFSKNSRYPSTRQKQFKPTMTPENDMEKIDPQSGEKMQLLTEQSVSSSDLLMLLGQNPTPHRLSLSDLQETRNEATDHLPGPTERIKGPSPSEVAHLRPELHHSGERVFTQEPELPVRLNENLGTTIMAELNKLNLKISSSSNNLMPLPTIPSDKLSAGTKKTGSFGPPNMPVNFNSQLGAIVFGKNLSHVIGSRVPLGLSKEDNNSKLLEAALMNSQESSLGENALSLESDRLFKEERVHGPVSLNKDDALFKVNISLVKTDKAPANSTTNRKTHIDDPTVLIENSTSVWQDIVIDSNTNFQEVTSLIHDELFMHKNTTSFGLSHMSNKTTSSKKREMIHQEKEGPKPLDTKYPDTPFFKTLFLPDSTNVIKRTHDKNSLSSGQRPSPKQLTSSVSENSVKDQNVLSEKNKAVAGEDEFSNNTSLKEMIFPNTKSIVLTNLANVQENDTDNQGKKSQEETEKKETLIPENTVLPQVYTVIGTKNFLKNLFSLSTKQNVRGLDEETYTPGLQDNRSLSDSAKRARIHAAHFSKGRVEANVEGLGNQTKQMVEKSPSTTKLSPIPSQQDAVPHRDKRGLKQSRLPREEVKLERGVTLNDTSTQWSKSMKHLTQGTFTQIEHNKMEKRTITQSFLPDCSLRSHRSIRPTDVTKIPPQHNSSHPPASTYGYNLGASHSGVQETSHFLHGAKKNNLSLAFLTLEVIGSQRKSSTPGKQATNPLMYKKLENMFLLKPGLSEASGKVVHQEDLPTKTSKVSPAHLDLREEIFLQKTQGLVKLNKVYRTGKVPSLKWTTESSEEIPSKLLGLLAWDNQSATLIPKEDWKSLERSHKLTSLKTKDITLPLDPSENYYSIAVTNEGEDKAQREAPWPKQGGTGRVCCQNPPILKRHQREITLTTFQPEPDKTDYDDTLSIETKREDFDIYGEDENQDPRSFQKRTRHYFIAAVERLWDYGMSRSPYALRNRSQNGGVPQFKKVVFEEFTDGSFTQAIYRGQLNEHLGLLGPYIRAEVEDNIMVTFKNQASRPYSFYSSLISYNGDQRQGAEPRKKFVKPNETQSYFWKVQHHMAPTKDEFDCKAWAYFSDVDLEKDLHSGLIGPILICRVDTLSAAHGRQVTIQEFALFFTIFDETKSWYFAENMARNCGAPCHVQPEDPTFQEKYRFHAINGYVMDTLPGLVMAQHQRIRWYLLSMGSNENIHSIHFSGHVFTVRKTEEYKMAVYNLYPGVFETVEMLPSKVGTWRIECLIGEHLQAGMSTLFLVYSKECQIPLGMASGRIRDFQITASGQYGQWAPKLARLHYSGSINAWSTKDPSPWIKVDLLAPMIIHSILTQGARQKFSSLYISQFIIMYSLDGQRWQGYRGNSTGTLMVFFGNVDSSGVKHNIFNPPIIARYIRLHPTHYSIRSTLRMELMGCDLNSCNMPLGMENKAISDTQITASSHLRNMFATWSPSQARLNLQGRTNAWRPQVNNPKEWLQVDFQKTMRVTGITTQGVKSLLTSMYVKEFLISSSQDGHNWTPFLQNGKVKVFQGNQDSFTPVVNALDPPLFTRFLRIHPRSWAHHIALRLELLGCEAQQQY

Target ORF information:

RefSeq Version	XM_005227639.4
Organism	Bos taurus(cattle)
Definition	Bos taurus coagulation factor VIII (F8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005227639.4

ORF Insert Sequence:

ATGCACATCA AGCTCTCTAC CTGCTTCTTT CTGTGCCTCT GGCCATGCAG CTTCAGTGCC 
ATCAGAAGAT ACTACCTGGG TGCAGTGGAA CTCTCCTGGG ACTATATGCA AAGTGAACTG 
CTCAGTGAGC TGCATGTGAA CACAAGGTTT CCTCCCAGAG TGCCCAAACC TTTTCCATTC 
AACACCTCAG TCATGTACAG AAAGACTGTG TTTGTAGAGT TCACGGATCA ACTTTTCAAC 
ATCGCCAAGC CCAGGCCACC ATGGATGGGT CTGCTGGGTC CAACTATCCA GGCTGAGGTT 
TATGACACCG TGGTCATTAC ATTTAAGAAT ATGGCTTCTC ATCCTGTTAG TCTTCATGCT 
ATTGGCGTAT CCTACTGGAA ATCTTCTGAA GGTGCTGCAT ATGAGGATGA AACCAGCCAA 
AGGGAGAAGG AAGATGACAA AGTCATTCCT GGTAAAAGTC ATACCTACGT CTGGCAGATC 
CTGAAAGAAA ATGGTCCAAC AGCCTCTGAC CCACCATGTC TCACCTACTC ATATCTTTCT 
CATGTGGACC TGGTGAAAGA CGTGAACTCA GGTCTCATTG GAGCCCTGCT AATTTGTAGG 
GAAGGAACTC TGATCAAAGA AAGGACACAG ACCTTGCACA AATTCGTACT ACTGTTTGCT 
GTATTTGATG AAGGGAAAAG TTGGCACTCG GGAAAAAATG AGTCCTTGAC ACGTGTTATG 
GATTCTGCAT CTGCACTGCA CACCATCAAT GGCTATATAA ACAGGTCTCT GCCAGGTCTG 
ATTGGATGTC ATAAGAAATC AGTCTATTGG CACGTGATTG GAATGGGCAC CACCCCGGAA 
GTGCACTCAA TTTTCCTTGA AGGCCACACA TTTCTCGTGA GGAACCATCG CCAGGCTTCC 
TTGGAGATCT CACCAATAAC TTTCCTTACC GCTCAGACAG TCCTGATGGA CTGTGGCCAG 
TTTCTACTGT TTTGTCGTAT CTCTTCCCAC CAACATGATG GTATGGAAGC TTATGTCAAA 
GTAGATAGTT GCCCAGAGGA ACCCCGACTA TGGATGAAAA ATAACCAAGA AGAAGATTAT 
GATGATGGTT TGGATGACTC TGACATGGAT GTGGTCAGGT TTGATGGCGA CAGTGCGCCT 
CCCTTTATCC AAATGCGCTC AGTTGCAAAG AAGCATCCTA AAACCTGGGT CCACTACATT 
GCTGCTGAAG AGGATGACTG GGACTATGCC CCCTCGGTCC TCACCACCAG TGACAGAAGT 
TATAAAAGTC TGTATCTGAA CCACGGTCCT CAGCGGATTG GTAGGAAGTA CAGAAAAGTA 
CGGTTTATAG CTTACACAGA TGGAACATTT AAGACTCGTG AAGCTATTCA GCATGAATCA 
GGGATCCTGG GGCCTTTACT TTATGGAGAA GTTGGAGACA CACTTTTGAT TATATTTAAG 
AATCAAGCAA GCCGGCCATA TAACATCTAC CCTCATGGAA TCACTGATGT CAGTCCTTTG 
CACTCAGGGA GATTTCCAAA AGGTGTGAAA CATTTGAAAG ACATGCCAAT TCTGCCAGGA 
GAAGTGTTCA AGTATAAATG GACAGTGACT GTAGAAGATG GGCCAACTAA ATCAGATCCT 
CGGTGTCTGA CCCGATATTA CTCGAGTTTC ATTAACTTAG AGAAAGATCT AGCTTCAGGA 
CTCATTGGCC CCCTCCTCAT CTGCTACAAA GAATCTGTAG ATCAAAGAGG AAACCAGATG 
ATGTCAGACA AGAGAAATGT CATCCTGTTT TCTGTATTTG ATGAGAATAA AAGTTGGTAC 
CTCACAGAGA ATATTCAGCG CTTCCTCCCC AATGGAGTAC AGCCCCAGGA TCCAGAGTTC 
CAAGTCTCCA ATGTCATGCA TAGCATCAAT GGCTATGTTT TTGATAGCTT GCAGCTGTCG 
GTTTGTTTGC ATGAGGTGGC ATACTGGTAC ATTCTAAGTG TTGGAGCCCA AATTGACTTC 
CTCTCTGTCT TCTTCTCTGG ATATACCTTC AAACACAAAA TGGTCTATGA AGATACACTC 
ACCCTATTCC CCTTTTCAGG AGAAACTGTC TTCATGTCAA TGGAAAATCC AGGTCTGTGG 
GTTCTGGGGT GCCACAACTC AGACTTTCGA AACAGAGGCA TGACAGCCTT ACTGAAGGTT 
GATAGTTGTG ACAGGAACGT TGGCGATTAT TATGACACAT ATGAAGATAT TCCAACCTTG 
CTGAGTGACG ACAATGTCAT TGAACCCAGA AGCTTCTCCA AGAATTCAAG GTACCCTAGC 
ACTAGGCAAA AGCAATTCAA ACCCACCATG ACTCCAGAAA ATGACATGGA GAAGATTGAC 
CCTCAGTCTG GAGAGAAAAT GCAGCTGCTT ACAGAACAAA GTGTCTCCTC TAGTGATCTG 
TTGATGCTCT TGGGACAGAA TCCTACTCCA CACAGATTAT CCTTATCTGA TCTCCAAGAA 
ACCAGAAATG AGGCTACTGA TCATTTACCT GGACCAACAG AAAGAATCAA GGGCCCATCC 
CCATCTGAAG TGGCACATCT TAGACCAGAG CTGCATCACA GTGGGGAAAG AGTATTTACT 
CAAGAGCCAG AACTGCCAGT AAGATTAAAT GAGAATTTGG GAACAACTAT AATGGCAGAG 
TTGAACAAAC TTAATTTAAA AATTTCAAGT TCATCAAACA ATCTAATGCC TTTACCAACA 
ATTCCATCAG ACAAGTTGTC AGCAGGTACT AAAAAGACAG GTTCCTTCGG ACCCCCAAAT 
ATGCCAGTTA ATTTTAATAG TCAATTAGGT GCCATTGTAT TTGGCAAAAA TTTATCTCAT 
GTTATTGGAT CACGTGTACC TTTGGGCTTG AGTAAAGAAG ATAATAATTC CAAGTTGTTA 
GAAGCAGCTT TAATGAATAG TCAAGAAAGT TCACTGGGAG AAAATGCATT ATCACTGGAG 
AGTGATAGGC TATTTAAAGA GGAAAGAGTT CATGGACCTG TGTCATTGAA CAAAGATGAT 
GCTTTATTTA AAGTGAATAT CTCTTTGGTA AAGACAGACA AGGCACCAGC TAACTCAACA 
ACTAATAGAA AGACTCACAT TGATGACCCA ACAGTATTAA TTGAGAATAG TACATCAGTC 
TGGCAGGATA TTGTAATAGA CAGTAATACC AACTTTCAAG AAGTGACTTC TTTGATTCAT 
GATGAATTGT TTATGCACAA AAATACTACA TCTTTTGGAC TAAGCCATAT GTCAAATAAA 
ACTACTTCAT CCAAAAAAAG GGAAATGATC CACCAAGAAA AAGAAGGCCC TAAGCCACTA 
GATACAAAAT ATCCAGATAC ACCATTCTTC AAGACTCTGT TTTTGCCAGA TTCAACAAAC 
GTGATAAAAA GGACCCACGA CAAGAACTCC CTAAGCTCTG GGCAAAGGCC CAGTCCAAAG 
CAATTAACAT CATCAGTATC AGAAAACTCT GTGAAAGATC AGAATGTCTT ATCAGAAAAG 
AATAAGGCAG TAGCAGGAGA GGATGAATTT AGCAACAACA CCAGCCTCAA AGAGATGATT 
TTTCCAAATA CCAAAAGCAT AGTTCTTACT AACTTGGCTA ATGTCCAAGA AAATGATACA 
GACAATCAAG GAAAAAAATC CCAGGAAGAA ACAGAAAAGA AAGAGACATT AATCCCTGAG 
AACACAGTTT TGCCTCAGGT GTATACAGTG ATTGGAACTA AGAATTTCCT GAAGAATCTC 
TTTTCACTGA GCACTAAGCA AAATGTCAGA GGTTTAGATG AAGAGACATA TACTCCAGGA 
CTTCAAGACA ACAGGTCATT AAGTGATTCG GCAAAAAGAG CCAGGATTCA TGCAGCCCAT 
TTCTCAAAAG GAAGGGTGGA AGCAAACGTG GAAGGCTTAG GGAATCAAAC AAAACAAATG 
GTAGAGAAAT CTCCAAGCAC TACAAAACTG TCTCCTATCC CAAGTCAGCA GGATGCAGTT 
CCTCACCGTG ATAAGCGGGG TTTGAAACAA TCCAGACTCC CACGAGAAGA AGTAAAGCTT 
GAAAGGGGGG TAACTCTGAA TGACACCTCA ACTCAGTGGT CCAAAAGCAT GAAACATTTG 
ACCCAGGGTA CCTTCACACA GATAGAACAC AACAAGATGG AGAAAAGGAC CATTACTCAG 
TCCTTCTTAC CAGATTGTTC TTTGAGGAGT CATAGGTCAA TTAGACCTAC AGATGTGACC 
AAGATCCCAC CTCAACATAA CTCTTCTCAT CCTCCAGCAT CAACCTATGG TTATAACTTG 
GGGGCAAGTC ATTCTGGGGT CCAAGAAACC AGTCATTTCT TACACGGAGC CAAGAAAAAT 
AATCTTTCTT TAGCCTTCCT AACATTGGAA GTGATCGGAA GTCAAAGAAA GAGCAGCACC 
CCGGGGAAAC AGGCCACAAA TCCACTCATG TACAAAAAAC TTGAGAACAT GTTTCTGTTG 
AAACCAGGCT TGTCTGAAGC ATCTGGGAAA GTTGTTCATC AGGAAGACTT GCCTACAAAA 
ACTAGCAAGG TTTCTCCTGC CCACCTGGAT CTCAGGGAAG AGATCTTCCT TCAGAAAACA 
CAGGGACTTG TTAAATTGAA TAAAGTCTAC AGGACTGGAA AAGTGCCATC TCTGAAATGG 
ACAACAGAAA GCTCTGAAGA GATTCCCTCC AAGCTGCTGG GTCTCCTTGC TTGGGATAAC 
CAGTCAGCTA CCTTGATACC AAAAGAAGAC TGGAAATCCC TAGAGAGGTC ACACAAACTC 
ACGTCTTTGA AGACAAAAGA TATCACTTTG CCCCTAGACC CTAGTGAAAA CTATTATTCA 
ATAGCAGTAA CAAATGAAGG AGAAGATAAG GCCCAAAGAG AAGCTCCCTG GCCAAAGCAA 
GGAGGGACTG GAAGGGTGTG CTGTCAAAAT CCACCAATCT TGAAACGCCA TCAAAGGGAG 
ATAACCCTTA CTACTTTTCA GCCAGAGCCA GACAAAACTG ACTATGATGA TACTTTGTCA 
ATTGAAACAA AGAGAGAAGA TTTTGACATT TATGGTGAAG ATGAAAATCA GGACCCCCGC 
AGCTTTCAAA AGAGAACACG CCACTATTTT ATTGCTGCAG TGGAGCGGCT CTGGGATTAT 
GGGATGAGTA GATCCCCCTA CGCACTAAGA AACAGGTCTC AGAATGGAGG AGTCCCTCAG 
TTCAAAAAGG TGGTATTCGA GGAATTTACT GATGGCTCCT TTACTCAGGC CATATACCGT 
GGACAACTAA ATGAACACCT GGGACTCTTG GGACCATATA TAAGAGCAGA AGTGGAAGAC 
AATATCATGG TAACTTTCAA AAACCAGGCC TCTCGTCCCT ACTCCTTCTA TTCTAGCCTT 
ATTTCTTATA ACGGAGATCA GAGACAAGGA GCAGAACCTC GAAAAAAGTT TGTCAAGCCT 
AATGAAACCC AAAGCTACTT TTGGAAAGTG CAGCACCATA TGGCACCCAC CAAAGATGAG 
TTTGACTGCA AAGCCTGGGC TTACTTTTCT GACGTTGATC TGGAAAAAGA TTTGCACTCA 
GGCTTGATTG GCCCCATTCT GATCTGCCGC GTGGACACGC TGAGTGCTGC TCATGGGAGA 
CAAGTAACAA TACAGGAATT CGCTCTGTTT TTCACCATTT TTGATGAAAC CAAGAGCTGG 
TACTTTGCCG AAAACATGGC AAGGAACTGC GGGGCGCCCT GCCATGTCCA GCCAGAGGAC 
CCTACTTTTC AAGAAAAGTA TCGCTTCCAT GCAATCAATG GCTACGTGAT GGATACACTC 
CCTGGCTTAG TCATGGCTCA GCATCAAAGG ATTAGGTGGT ATCTGCTCAG CATGGGCAGC 
AATGAAAATA TCCATTCCAT TCATTTCAGT GGCCATGTGT TCACTGTGCG AAAAACGGAG 
GAGTATAAAA TGGCGGTCTA CAATCTCTAC CCAGGTGTCT TTGAGACGGT GGAAATGCTA 
CCATCCAAGG TTGGGACTTG GCGGATAGAA TGTCTTATTG GCGAGCACCT ACAAGCTGGG 
ATGAGCACTC TCTTCCTGGT GTACAGCAAG GAGTGTCAAA TTCCACTGGG AATGGCTTCT 
GGACGCATTA GAGATTTTCA GATTACAGCT TCAGGACAAT ATGGACAGTG GGCCCCCAAG 
CTGGCCAGAC TTCATTATTC TGGATCAATC AACGCCTGGA GCACCAAGGA TCCCTCTCCT 
TGGATCAAGG TGGATCTGTT GGCGCCGATG ATTATTCACA GCATCCTGAC TCAGGGTGCC 
CGGCAGAAGT TCTCCAGCCT GTACATCTCT CAGTTTATCA TCATGTACAG CCTCGATGGA 
CAGCGGTGGC AGGGTTATCG GGGGAACTCC ACTGGGACCT TAATGGTGTT CTTTGGTAAT 
GTGGATTCAT CTGGAGTAAA ACACAATATT TTTAACCCTC CAATTATTGC TAGATATATC 
CGTTTACACC CAACTCATTA CAGCATCCGC AGCACTCTTC GCATGGAGTT GATGGGCTGT 
GATTTAAACA GTTGCAACAT GCCACTGGGA ATGGAGAATA AAGCTATATC AGATACACAG 
ATTACTGCCT CATCCCACTT ACGCAACATG TTTGCCACCT GGTCTCCTTC ACAAGCCCGA 
CTTAACCTTC AAGGGAGGAC AAATGCCTGG AGGCCCCAGG TGAATAATCC AAAAGAGTGG 
CTACAAGTGG ACTTCCAGAA GACAATGAGA GTTACAGGAA TAACTACCCA AGGGGTGAAA 
TCTCTGCTTA CCAGCATGTA TGTGAAGGAG TTCCTCATAT CCAGTAGTCA AGATGGCCAT 
AACTGGACTC CATTTCTTCA GAATGGCAAA GTGAAGGTTT TTCAGGGAAA TCAAGACTCC 
TTCACCCCCG TGGTGAATGC TCTAGACCCC CCGCTGTTTA CCCGCTTCCT TCGGATTCAC 
CCGCGGAGCT GGGCGCACCA TATCGCCCTG AGGCTGGAGC TTTTGGGCTG CGAGGCACAG 
CAGCAGTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

F8 ( XM_024987717.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024843485.1 Bos taurus coagulation factor VIII (F8), transcript variant X2, mRNA.

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CloneID	OBa35243
Clone ID Related Accession (Same CDS sequence)	XM_005227639.3 , XM_005227639.4 , XM_024987717.1
Accession Version	XM_024987717.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	coagulation factor VIII isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACATCA AGCTCTCTAC CTGCTTCTTT CTGTGCCTCT GGCCATGCAG CTTCAGTGCC 
ATCAGAAGAT ACTACCTGGG TGCAGTGGAA CTCTCCTGGG ACTATATGCA AAGTGAACTG 
CTCAGTGAGC TGCATGTGAA CACAAGGTTT CCTCCCAGAG TGCCCAAACC TTTTCCATTC 
AACACCTCAG TCATGTACAG AAAGACTGTG TTTGTAGAGT TCACGGATCA ACTTTTCAAC 
ATCGCCAAGC CCAGGCCACC ATGGATGGGT CTGCTGGGTC CAACTATCCA GGCTGAGGTT 
TATGACACCG TGGTCATTAC ATTTAAGAAT ATGGCTTCTC ATCCTGTTAG TCTTCATGCT 
ATTGGCGTAT CCTACTGGAA ATCTTCTGAA GGTGCTGCAT ATGAGGATGA AACCAGCCAA 
AGGGAGAAGG AAGATGACAA AGTCATTCCT GGTAAAAGTC ATACCTACGT CTGGCAGATC 
CTGAAAGAAA ATGGTCCAAC AGCCTCTGAC CCACCATGTC TCACCTACTC ATATCTTTCT 
CATGTGGACC TGGTGAAAGA CGTGAACTCA GGTCTCATTG GAGCCCTGCT AATTTGTAGG 
GAAGGAACTC TGATCAAAGA AAGGACACAG ACCTTGCACA AATTCGTACT ACTGTTTGCT 
GTATTTGATG AAGGGAAAAG TTGGCACTCG GGAAAAAATG AGTCCTTGAC ACGTGTTATG 
GATTCTGCAT CTGCACTGCA CACCATCAAT GGCTATATAA ACAGGTCTCT GCCAGGTCTG 
ATTGGATGTC ATAAGAAATC AGTCTATTGG CACGTGATTG GAATGGGCAC CACCCCGGAA 
GTGCACTCAA TTTTCCTTGA AGGCCACACA TTTCTCGTGA GGAACCATCG CCAGGCTTCC 
TTGGAGATCT CACCAATAAC TTTCCTTACC GCTCAGACAG TCCTGATGGA CTGTGGCCAG 
TTTCTACTGT TTTGTCGTAT CTCTTCCCAC CAACATGATG GTATGGAAGC TTATGTCAAA 
GTAGATAGTT GCCCAGAGGA ACCCCGACTA TGGATGAAAA ATAACCAAGA AGAAGATTAT 
GATGATGGTT TGGATGACTC TGACATGGAT GTGGTCAGGT TTGATGGCGA CAGTGCGCCT 
CCCTTTATCC AAATGCGCTC AGTTGCAAAG AAGCATCCTA AAACCTGGGT CCACTACATT 
GCTGCTGAAG AGGATGACTG GGACTATGCC CCCTCGGTCC TCACCACCAG TGACAGAAGT 
TATAAAAGTC TGTATCTGAA CCACGGTCCT CAGCGGATTG GTAGGAAGTA CAGAAAAGTA 
CGGTTTATAG CTTACACAGA TGGAACATTT AAGACTCGTG AAGCTATTCA GCATGAATCA 
GGGATCCTGG GGCCTTTACT TTATGGAGAA GTTGGAGACA CACTTTTGAT TATATTTAAG 
AATCAAGCAA GCCGGCCATA TAACATCTAC CCTCATGGAA TCACTGATGT CAGTCCTTTG 
CACTCAGGGA GATTTCCAAA AGGTGTGAAA CATTTGAAAG ACATGCCAAT TCTGCCAGGA 
GAAGTGTTCA AGTATAAATG GACAGTGACT GTAGAAGATG GGCCAACTAA ATCAGATCCT 
CGGTGTCTGA CCCGATATTA CTCGAGTTTC ATTAACTTAG AGAAAGATCT AGCTTCAGGA 
CTCATTGGCC CCCTCCTCAT CTGCTACAAA GAATCTGTAG ATCAAAGAGG AAACCAGATG 
ATGTCAGACA AGAGAAATGT CATCCTGTTT TCTGTATTTG ATGAGAATAA AAGTTGGTAC 
CTCACAGAGA ATATTCAGCG CTTCCTCCCC AATGGAGTAC AGCCCCAGGA TCCAGAGTTC 
CAAGTCTCCA ATGTCATGCA TAGCATCAAT GGCTATGTTT TTGATAGCTT GCAGCTGTCG 
GTTTGTTTGC ATGAGGTGGC ATACTGGTAC ATTCTAAGTG TTGGAGCCCA AATTGACTTC 
CTCTCTGTCT TCTTCTCTGG ATATACCTTC AAACACAAAA TGGTCTATGA AGATACACTC 
ACCCTATTCC CCTTTTCAGG AGAAACTGTC TTCATGTCAA TGGAAAATCC AGGTCTGTGG 
GTTCTGGGGT GCCACAACTC AGACTTTCGA AACAGAGGCA TGACAGCCTT ACTGAAGGTT 
GATAGTTGTG ACAGGAACGT TGGCGATTAT TATGACACAT ATGAAGATAT TCCAACCTTG 
CTGAGTGACG ACAATGTCAT TGAACCCAGA AGCTTCTCCA AGAATTCAAG GTACCCTAGC 
ACTAGGCAAA AGCAATTCAA ACCCACCATG ACTCCAGAAA ATGACATGGA GAAGATTGAC 
CCTCAGTCTG GAGAGAAAAT GCAGCTGCTT ACAGAACAAA GTGTCTCCTC TAGTGATCTG 
TTGATGCTCT TGGGACAGAA TCCTACTCCA CACAGATTAT CCTTATCTGA TCTCCAAGAA 
ACCAGAAATG AGGCTACTGA TCATTTACCT GGACCAACAG AAAGAATCAA GGGCCCATCC 
CCATCTGAAG TGGCACATCT TAGACCAGAG CTGCATCACA GTGGGGAAAG AGTATTTACT 
CAAGAGCCAG AACTGCCAGT AAGATTAAAT GAGAATTTGG GAACAACTAT AATGGCAGAG 
TTGAACAAAC TTAATTTAAA AATTTCAAGT TCATCAAACA ATCTAATGCC TTTACCAACA 
ATTCCATCAG ACAAGTTGTC AGCAGGTACT AAAAAGACAG GTTCCTTCGG ACCCCCAAAT 
ATGCCAGTTA ATTTTAATAG TCAATTAGGT GCCATTGTAT TTGGCAAAAA TTTATCTCAT 
GTTATTGGAT CACGTGTACC TTTGGGCTTG AGTAAAGAAG ATAATAATTC CAAGTTGTTA 
GAAGCAGCTT TAATGAATAG TCAAGAAAGT TCACTGGGAG AAAATGCATT ATCACTGGAG 
AGTGATAGGC TATTTAAAGA GGAAAGAGTT CATGGACCTG TGTCATTGAA CAAAGATGAT 
GCTTTATTTA AAGTGAATAT CTCTTTGGTA AAGACAGACA AGGCACCAGC TAACTCAACA 
ACTAATAGAA AGACTCACAT TGATGACCCA ACAGTATTAA TTGAGAATAG TACATCAGTC 
TGGCAGGATA TTGTAATAGA CAGTAATACC AACTTTCAAG AAGTGACTTC TTTGATTCAT 
GATGAATTGT TTATGCACAA AAATACTACA TCTTTTGGAC TAAGCCATAT GTCAAATAAA 
ACTACTTCAT CCAAAAAAAG GGAAATGATC CACCAAGAAA AAGAAGGCCC TAAGCCACTA 
GATACAAAAT ATCCAGATAC ACCATTCTTC AAGACTCTGT TTTTGCCAGA TTCAACAAAC 
GTGATAAAAA GGACCCACGA CAAGAACTCC CTAAGCTCTG GGCAAAGGCC CAGTCCAAAG 
CAATTAACAT CATCAGTATC AGAAAACTCT GTGAAAGATC AGAATGTCTT ATCAGAAAAG 
AATAAGGCAG TAGCAGGAGA GGATGAATTT AGCAACAACA CCAGCCTCAA AGAGATGATT 
TTTCCAAATA CCAAAAGCAT AGTTCTTACT AACTTGGCTA ATGTCCAAGA AAATGATACA 
GACAATCAAG GAAAAAAATC CCAGGAAGAA ACAGAAAAGA AAGAGACATT AATCCCTGAG 
AACACAGTTT TGCCTCAGGT GTATACAGTG ATTGGAACTA AGAATTTCCT GAAGAATCTC 
TTTTCACTGA GCACTAAGCA AAATGTCAGA GGTTTAGATG AAGAGACATA TACTCCAGGA 
CTTCAAGACA ACAGGTCATT AAGTGATTCG GCAAAAAGAG CCAGGATTCA TGCAGCCCAT 
TTCTCAAAAG GAAGGGTGGA AGCAAACGTG GAAGGCTTAG GGAATCAAAC AAAACAAATG 
GTAGAGAAAT CTCCAAGCAC TACAAAACTG TCTCCTATCC CAAGTCAGCA GGATGCAGTT 
CCTCACCGTG ATAAGCGGGG TTTGAAACAA TCCAGACTCC CACGAGAAGA AGTAAAGCTT 
GAAAGGGGGG TAACTCTGAA TGACACCTCA ACTCAGTGGT CCAAAAGCAT GAAACATTTG 
ACCCAGGGTA CCTTCACACA GATAGAACAC AACAAGATGG AGAAAAGGAC CATTACTCAG 
TCCTTCTTAC CAGATTGTTC TTTGAGGAGT CATAGGTCAA TTAGACCTAC AGATGTGACC 
AAGATCCCAC CTCAACATAA CTCTTCTCAT CCTCCAGCAT CAACCTATGG TTATAACTTG 
GGGGCAAGTC ATTCTGGGGT CCAAGAAACC AGTCATTTCT TACACGGAGC CAAGAAAAAT 
AATCTTTCTT TAGCCTTCCT AACATTGGAA GTGATCGGAA GTCAAAGAAA GAGCAGCACC 
CCGGGGAAAC AGGCCACAAA TCCACTCATG TACAAAAAAC TTGAGAACAT GTTTCTGTTG 
AAACCAGGCT TGTCTGAAGC ATCTGGGAAA GTTGTTCATC AGGAAGACTT GCCTACAAAA 
ACTAGCAAGG TTTCTCCTGC CCACCTGGAT CTCAGGGAAG AGATCTTCCT TCAGAAAACA 
CAGGGACTTG TTAAATTGAA TAAAGTCTAC AGGACTGGAA AAGTGCCATC TCTGAAATGG 
ACAACAGAAA GCTCTGAAGA GATTCCCTCC AAGCTGCTGG GTCTCCTTGC TTGGGATAAC 
CAGTCAGCTA CCTTGATACC AAAAGAAGAC TGGAAATCCC TAGAGAGGTC ACACAAACTC 
ACGTCTTTGA AGACAAAAGA TATCACTTTG CCCCTAGACC CTAGTGAAAA CTATTATTCA 
ATAGCAGTAA CAAATGAAGG AGAAGATAAG GCCCAAAGAG AAGCTCCCTG GCCAAAGCAA 
GGAGGGACTG GAAGGGTGTG CTGTCAAAAT CCACCAATCT TGAAACGCCA TCAAAGGGAG 
ATAACCCTTA CTACTTTTCA GCCAGAGCCA GACAAAACTG ACTATGATGA TACTTTGTCA 
ATTGAAACAA AGAGAGAAGA TTTTGACATT TATGGTGAAG ATGAAAATCA GGACCCCCGC 
AGCTTTCAAA AGAGAACACG CCACTATTTT ATTGCTGCAG TGGAGCGGCT CTGGGATTAT 
GGGATGAGTA GATCCCCCTA CGCACTAAGA AACAGGTCTC AGAATGGAGG AGTCCCTCAG 
TTCAAAAAGG TGGTATTCGA GGAATTTACT GATGGCTCCT TTACTCAGGC CATATACCGT 
GGACAACTAA ATGAACACCT GGGACTCTTG GGACCATATA TAAGAGCAGA AGTGGAAGAC 
AATATCATGG TAACTTTCAA AAACCAGGCC TCTCGTCCCT ACTCCTTCTA TTCTAGCCTT 
ATTTCTTATA ACGGAGATCA GAGACAAGGA GCAGAACCTC GAAAAAAGTT TGTCAAGCCT 
AATGAAACCC AAAGCTACTT TTGGAAAGTG CAGCACCATA TGGCACCCAC CAAAGATGAG 
TTTGACTGCA AAGCCTGGGC TTACTTTTCT GACGTTGATC TGGAAAAAGA TTTGCACTCA 
GGCTTGATTG GCCCCATTCT GATCTGCCGC GTGGACACGC TGAGTGCTGC TCATGGGAGA 
CAAGTAACAA TACAGGAATT CGCTCTGTTT TTCACCATTT TTGATGAAAC CAAGAGCTGG 
TACTTTGCCG AAAACATGGC AAGGAACTGC GGGGCGCCCT GCCATGTCCA GCCAGAGGAC 
CCTACTTTTC AAGAAAAGTA TCGCTTCCAT GCAATCAATG GCTACGTGAT GGATACACTC 
CCTGGCTTAG TCATGGCTCA GCATCAAAGG ATTAGGTGGT ATCTGCTCAG CATGGGCAGC 
AATGAAAATA TCCATTCCAT TCATTTCAGT GGCCATGTGT TCACTGTGCG AAAAACGGAG 
GAGTATAAAA TGGCGGTCTA CAATCTCTAC CCAGGTGTCT TTGAGACGGT GGAAATGCTA 
CCATCCAAGG TTGGGACTTG GCGGATAGAA TGTCTTATTG GCGAGCACCT ACAAGCTGGG 
ATGAGCACTC TCTTCCTGGT GTACAGCAAG GAGTGTCAAA TTCCACTGGG AATGGCTTCT 
GGACGCATTA GAGATTTTCA GATTACAGCT TCAGGACAAT ATGGACAGTG GGCCCCCAAG 
CTGGCCAGAC TTCATTATTC TGGATCAATC AACGCCTGGA GCACCAAGGA TCCCTCTCCT 
TGGATCAAGG TGGATCTGTT GGCGCCGATG ATTATTCACA GCATCCTGAC TCAGGGTGCC 
CGGCAGAAGT TCTCCAGCCT GTACATCTCT CAGTTTATCA TCATGTACAG CCTCGATGGA 
CAGCGGTGGC AGGGTTATCG GGGGAACTCC ACTGGGACCT TAATGGTGTT CTTTGGTAAT 
GTGGATTCAT CTGGAGTAAA ACACAATATT TTTAACCCTC CAATTATTGC TAGATATATC 
CGTTTACACC CAACTCATTA CAGCATCCGC AGCACTCTTC GCATGGAGTT GATGGGCTGT 
GATTTAAACA GTTGCAACAT GCCACTGGGA ATGGAGAATA AAGCTATATC AGATACACAG 
ATTACTGCCT CATCCCACTT ACGCAACATG TTTGCCACCT GGTCTCCTTC ACAAGCCCGA 
CTTAACCTTC AAGGGAGGAC AAATGCCTGG AGGCCCCAGG TGAATAATCC AAAAGAGTGG 
CTACAAGTGG ACTTCCAGAA GACAATGAGA GTTACAGGAA TAACTACCCA AGGGGTGAAA 
TCTCTGCTTA CCAGCATGTA TGTGAAGGAG TTCCTCATAT CCAGTAGTCA AGATGGCCAT 
AACTGGACTC CATTTCTTCA GAATGGCAAA GTGAAGGTTT TTCAGGGAAA TCAAGACTCC 
TTCACCCCCG TGGTGAATGC TCTAGACCCC CCGCTGTTTA CCCGCTTCCT TCGGATTCAC 
CCGCGGAGCT GGGCGCACCA TATCGCCCTG AGGCTGGAGC TTTTGGGCTG CGAGGCACAG 
CAGCAGTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024843485.1
CDS	157..7128
Translation	MHIKLSTCFFLCLWPCSFSAIRRYYLGAVELSWDYMQSELLSELHVNTRFPPRVPKPFPFNTSVMYRKTVFVEFTDQLFNIAKPRPPWMGLLGPTIQAEVYDTVVITFKNMASHPVSLHAIGVSYWKSSEGAAYEDETSQREKEDDKVIPGKSHTYVWQILKENGPTASDPPCLTYSYLSHVDLVKDVNSGLIGALLICREGTLIKERTQTLHKFVLLFAVFDEGKSWHSGKNESLTRVMDSASALHTINGYINRSLPGLIGCHKKSVYWHVIGMGTTPEVHSIFLEGHTFLVRNHRQASLEISPITFLTAQTVLMDCGQFLLFCRISSHQHDGMEAYVKVDSCPEEPRLWMKNNQEEDYDDGLDDSDMDVVRFDGDSAPPFIQMRSVAKKHPKTWVHYIAAEEDDWDYAPSVLTTSDRSYKSLYLNHGPQRIGRKYRKVRFIAYTDGTFKTREAIQHESGILGPLLYGEVGDTLLIIFKNQASRPYNIYPHGITDVSPLHSGRFPKGVKHLKDMPILPGEVFKYKWTVTVEDGPTKSDPRCLTRYYSSFINLEKDLASGLIGPLLICYKESVDQRGNQMMSDKRNVILFSVFDENKSWYLTENIQRFLPNGVQPQDPEFQVSNVMHSINGYVFDSLQLSVCLHEVAYWYILSVGAQIDFLSVFFSGYTFKHKMVYEDTLTLFPFSGETVFMSMENPGLWVLGCHNSDFRNRGMTALLKVDSCDRNVGDYYDTYEDIPTLLSDDNVIEPRSFSKNSRYPSTRQKQFKPTMTPENDMEKIDPQSGEKMQLLTEQSVSSSDLLMLLGQNPTPHRLSLSDLQETRNEATDHLPGPTERIKGPSPSEVAHLRPELHHSGERVFTQEPELPVRLNENLGTTIMAELNKLNLKISSSSNNLMPLPTIPSDKLSAGTKKTGSFGPPNMPVNFNSQLGAIVFGKNLSHVIGSRVPLGLSKEDNNSKLLEAALMNSQESSLGENALSLESDRLFKEERVHGPVSLNKDDALFKVNISLVKTDKAPANSTTNRKTHIDDPTVLIENSTSVWQDIVIDSNTNFQEVTSLIHDELFMHKNTTSFGLSHMSNKTTSSKKREMIHQEKEGPKPLDTKYPDTPFFKTLFLPDSTNVIKRTHDKNSLSSGQRPSPKQLTSSVSENSVKDQNVLSEKNKAVAGEDEFSNNTSLKEMIFPNTKSIVLTNLANVQENDTDNQGKKSQEETEKKETLIPENTVLPQVYTVIGTKNFLKNLFSLSTKQNVRGLDEETYTPGLQDNRSLSDSAKRARIHAAHFSKGRVEANVEGLGNQTKQMVEKSPSTTKLSPIPSQQDAVPHRDKRGLKQSRLPREEVKLERGVTLNDTSTQWSKSMKHLTQGTFTQIEHNKMEKRTITQSFLPDCSLRSHRSIRPTDVTKIPPQHNSSHPPASTYGYNLGASHSGVQETSHFLHGAKKNNLSLAFLTLEVIGSQRKSSTPGKQATNPLMYKKLENMFLLKPGLSEASGKVVHQEDLPTKTSKVSPAHLDLREEIFLQKTQGLVKLNKVYRTGKVPSLKWTTESSEEIPSKLLGLLAWDNQSATLIPKEDWKSLERSHKLTSLKTKDITLPLDPSENYYSIAVTNEGEDKAQREAPWPKQGGTGRVCCQNPPILKRHQREITLTTFQPEPDKTDYDDTLSIETKREDFDIYGEDENQDPRSFQKRTRHYFIAAVERLWDYGMSRSPYALRNRSQNGGVPQFKKVVFEEFTDGSFTQAIYRGQLNEHLGLLGPYIRAEVEDNIMVTFKNQASRPYSFYSSLISYNGDQRQGAEPRKKFVKPNETQSYFWKVQHHMAPTKDEFDCKAWAYFSDVDLEKDLHSGLIGPILICRVDTLSAAHGRQVTIQEFALFFTIFDETKSWYFAENMARNCGAPCHVQPEDPTFQEKYRFHAINGYVMDTLPGLVMAQHQRIRWYLLSMGSNENIHSIHFSGHVFTVRKTEEYKMAVYNLYPGVFETVEMLPSKVGTWRIECLIGEHLQAGMSTLFLVYSKECQIPLGMASGRIRDFQITASGQYGQWAPKLARLHYSGSINAWSTKDPSPWIKVDLLAPMIIHSILTQGARQKFSSLYISQFIIMYSLDGQRWQGYRGNSTGTLMVFFGNVDSSGVKHNIFNPPIIARYIRLHPTHYSIRSTLRMELMGCDLNSCNMPLGMENKAISDTQITASSHLRNMFATWSPSQARLNLQGRTNAWRPQVNNPKEWLQVDFQKTMRVTGITTQGVKSLLTSMYVKEFLISSSQDGHNWTPFLQNGKVKVFQGNQDSFTPVVNALDPPLFTRFLRIHPRSWAHHIALRLELLGCEAQQQY

Target ORF information:

RefSeq Version	XM_024987717.1
Organism	Bos taurus(cattle)
Definition	Bos taurus coagulation factor VIII (F8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024987717.1

ORF Insert Sequence:

ATGCACATCA AGCTCTCTAC CTGCTTCTTT CTGTGCCTCT GGCCATGCAG CTTCAGTGCC 
ATCAGAAGAT ACTACCTGGG TGCAGTGGAA CTCTCCTGGG ACTATATGCA AAGTGAACTG 
CTCAGTGAGC TGCATGTGAA CACAAGGTTT CCTCCCAGAG TGCCCAAACC TTTTCCATTC 
AACACCTCAG TCATGTACAG AAAGACTGTG TTTGTAGAGT TCACGGATCA ACTTTTCAAC 
ATCGCCAAGC CCAGGCCACC ATGGATGGGT CTGCTGGGTC CAACTATCCA GGCTGAGGTT 
TATGACACCG TGGTCATTAC ATTTAAGAAT ATGGCTTCTC ATCCTGTTAG TCTTCATGCT 
ATTGGCGTAT CCTACTGGAA ATCTTCTGAA GGTGCTGCAT ATGAGGATGA AACCAGCCAA 
AGGGAGAAGG AAGATGACAA AGTCATTCCT GGTAAAAGTC ATACCTACGT CTGGCAGATC 
CTGAAAGAAA ATGGTCCAAC AGCCTCTGAC CCACCATGTC TCACCTACTC ATATCTTTCT 
CATGTGGACC TGGTGAAAGA CGTGAACTCA GGTCTCATTG GAGCCCTGCT AATTTGTAGG 
GAAGGAACTC TGATCAAAGA AAGGACACAG ACCTTGCACA AATTCGTACT ACTGTTTGCT 
GTATTTGATG AAGGGAAAAG TTGGCACTCG GGAAAAAATG AGTCCTTGAC ACGTGTTATG 
GATTCTGCAT CTGCACTGCA CACCATCAAT GGCTATATAA ACAGGTCTCT GCCAGGTCTG 
ATTGGATGTC ATAAGAAATC AGTCTATTGG CACGTGATTG GAATGGGCAC CACCCCGGAA 
GTGCACTCAA TTTTCCTTGA AGGCCACACA TTTCTCGTGA GGAACCATCG CCAGGCTTCC 
TTGGAGATCT CACCAATAAC TTTCCTTACC GCTCAGACAG TCCTGATGGA CTGTGGCCAG 
TTTCTACTGT TTTGTCGTAT CTCTTCCCAC CAACATGATG GTATGGAAGC TTATGTCAAA 
GTAGATAGTT GCCCAGAGGA ACCCCGACTA TGGATGAAAA ATAACCAAGA AGAAGATTAT 
GATGATGGTT TGGATGACTC TGACATGGAT GTGGTCAGGT TTGATGGCGA CAGTGCGCCT 
CCCTTTATCC AAATGCGCTC AGTTGCAAAG AAGCATCCTA AAACCTGGGT CCACTACATT 
GCTGCTGAAG AGGATGACTG GGACTATGCC CCCTCGGTCC TCACCACCAG TGACAGAAGT 
TATAAAAGTC TGTATCTGAA CCACGGTCCT CAGCGGATTG GTAGGAAGTA CAGAAAAGTA 
CGGTTTATAG CTTACACAGA TGGAACATTT AAGACTCGTG AAGCTATTCA GCATGAATCA 
GGGATCCTGG GGCCTTTACT TTATGGAGAA GTTGGAGACA CACTTTTGAT TATATTTAAG 
AATCAAGCAA GCCGGCCATA TAACATCTAC CCTCATGGAA TCACTGATGT CAGTCCTTTG 
CACTCAGGGA GATTTCCAAA AGGTGTGAAA CATTTGAAAG ACATGCCAAT TCTGCCAGGA 
GAAGTGTTCA AGTATAAATG GACAGTGACT GTAGAAGATG GGCCAACTAA ATCAGATCCT 
CGGTGTCTGA CCCGATATTA CTCGAGTTTC ATTAACTTAG AGAAAGATCT AGCTTCAGGA 
CTCATTGGCC CCCTCCTCAT CTGCTACAAA GAATCTGTAG ATCAAAGAGG AAACCAGATG 
ATGTCAGACA AGAGAAATGT CATCCTGTTT TCTGTATTTG ATGAGAATAA AAGTTGGTAC 
CTCACAGAGA ATATTCAGCG CTTCCTCCCC AATGGAGTAC AGCCCCAGGA TCCAGAGTTC 
CAAGTCTCCA ATGTCATGCA TAGCATCAAT GGCTATGTTT TTGATAGCTT GCAGCTGTCG 
GTTTGTTTGC ATGAGGTGGC ATACTGGTAC ATTCTAAGTG TTGGAGCCCA AATTGACTTC 
CTCTCTGTCT TCTTCTCTGG ATATACCTTC AAACACAAAA TGGTCTATGA AGATACACTC 
ACCCTATTCC CCTTTTCAGG AGAAACTGTC TTCATGTCAA TGGAAAATCC AGGTCTGTGG 
GTTCTGGGGT GCCACAACTC AGACTTTCGA AACAGAGGCA TGACAGCCTT ACTGAAGGTT 
GATAGTTGTG ACAGGAACGT TGGCGATTAT TATGACACAT ATGAAGATAT TCCAACCTTG 
CTGAGTGACG ACAATGTCAT TGAACCCAGA AGCTTCTCCA AGAATTCAAG GTACCCTAGC 
ACTAGGCAAA AGCAATTCAA ACCCACCATG ACTCCAGAAA ATGACATGGA GAAGATTGAC 
CCTCAGTCTG GAGAGAAAAT GCAGCTGCTT ACAGAACAAA GTGTCTCCTC TAGTGATCTG 
TTGATGCTCT TGGGACAGAA TCCTACTCCA CACAGATTAT CCTTATCTGA TCTCCAAGAA 
ACCAGAAATG AGGCTACTGA TCATTTACCT GGACCAACAG AAAGAATCAA GGGCCCATCC 
CCATCTGAAG TGGCACATCT TAGACCAGAG CTGCATCACA GTGGGGAAAG AGTATTTACT 
CAAGAGCCAG AACTGCCAGT AAGATTAAAT GAGAATTTGG GAACAACTAT AATGGCAGAG 
TTGAACAAAC TTAATTTAAA AATTTCAAGT TCATCAAACA ATCTAATGCC TTTACCAACA 
ATTCCATCAG ACAAGTTGTC AGCAGGTACT AAAAAGACAG GTTCCTTCGG ACCCCCAAAT 
ATGCCAGTTA ATTTTAATAG TCAATTAGGT GCCATTGTAT TTGGCAAAAA TTTATCTCAT 
GTTATTGGAT CACGTGTACC TTTGGGCTTG AGTAAAGAAG ATAATAATTC CAAGTTGTTA 
GAAGCAGCTT TAATGAATAG TCAAGAAAGT TCACTGGGAG AAAATGCATT ATCACTGGAG 
AGTGATAGGC TATTTAAAGA GGAAAGAGTT CATGGACCTG TGTCATTGAA CAAAGATGAT 
GCTTTATTTA AAGTGAATAT CTCTTTGGTA AAGACAGACA AGGCACCAGC TAACTCAACA 
ACTAATAGAA AGACTCACAT TGATGACCCA ACAGTATTAA TTGAGAATAG TACATCAGTC 
TGGCAGGATA TTGTAATAGA CAGTAATACC AACTTTCAAG AAGTGACTTC TTTGATTCAT 
GATGAATTGT TTATGCACAA AAATACTACA TCTTTTGGAC TAAGCCATAT GTCAAATAAA 
ACTACTTCAT CCAAAAAAAG GGAAATGATC CACCAAGAAA AAGAAGGCCC TAAGCCACTA 
GATACAAAAT ATCCAGATAC ACCATTCTTC AAGACTCTGT TTTTGCCAGA TTCAACAAAC 
GTGATAAAAA GGACCCACGA CAAGAACTCC CTAAGCTCTG GGCAAAGGCC CAGTCCAAAG 
CAATTAACAT CATCAGTATC AGAAAACTCT GTGAAAGATC AGAATGTCTT ATCAGAAAAG 
AATAAGGCAG TAGCAGGAGA GGATGAATTT AGCAACAACA CCAGCCTCAA AGAGATGATT 
TTTCCAAATA CCAAAAGCAT AGTTCTTACT AACTTGGCTA ATGTCCAAGA AAATGATACA 
GACAATCAAG GAAAAAAATC CCAGGAAGAA ACAGAAAAGA AAGAGACATT AATCCCTGAG 
AACACAGTTT TGCCTCAGGT GTATACAGTG ATTGGAACTA AGAATTTCCT GAAGAATCTC 
TTTTCACTGA GCACTAAGCA AAATGTCAGA GGTTTAGATG AAGAGACATA TACTCCAGGA 
CTTCAAGACA ACAGGTCATT AAGTGATTCG GCAAAAAGAG CCAGGATTCA TGCAGCCCAT 
TTCTCAAAAG GAAGGGTGGA AGCAAACGTG GAAGGCTTAG GGAATCAAAC AAAACAAATG 
GTAGAGAAAT CTCCAAGCAC TACAAAACTG TCTCCTATCC CAAGTCAGCA GGATGCAGTT 
CCTCACCGTG ATAAGCGGGG TTTGAAACAA TCCAGACTCC CACGAGAAGA AGTAAAGCTT 
GAAAGGGGGG TAACTCTGAA TGACACCTCA ACTCAGTGGT CCAAAAGCAT GAAACATTTG 
ACCCAGGGTA CCTTCACACA GATAGAACAC AACAAGATGG AGAAAAGGAC CATTACTCAG 
TCCTTCTTAC CAGATTGTTC TTTGAGGAGT CATAGGTCAA TTAGACCTAC AGATGTGACC 
AAGATCCCAC CTCAACATAA CTCTTCTCAT CCTCCAGCAT CAACCTATGG TTATAACTTG 
GGGGCAAGTC ATTCTGGGGT CCAAGAAACC AGTCATTTCT TACACGGAGC CAAGAAAAAT 
AATCTTTCTT TAGCCTTCCT AACATTGGAA GTGATCGGAA GTCAAAGAAA GAGCAGCACC 
CCGGGGAAAC AGGCCACAAA TCCACTCATG TACAAAAAAC TTGAGAACAT GTTTCTGTTG 
AAACCAGGCT TGTCTGAAGC ATCTGGGAAA GTTGTTCATC AGGAAGACTT GCCTACAAAA 
ACTAGCAAGG TTTCTCCTGC CCACCTGGAT CTCAGGGAAG AGATCTTCCT TCAGAAAACA 
CAGGGACTTG TTAAATTGAA TAAAGTCTAC AGGACTGGAA AAGTGCCATC TCTGAAATGG 
ACAACAGAAA GCTCTGAAGA GATTCCCTCC AAGCTGCTGG GTCTCCTTGC TTGGGATAAC 
CAGTCAGCTA CCTTGATACC AAAAGAAGAC TGGAAATCCC TAGAGAGGTC ACACAAACTC 
ACGTCTTTGA AGACAAAAGA TATCACTTTG CCCCTAGACC CTAGTGAAAA CTATTATTCA 
ATAGCAGTAA CAAATGAAGG AGAAGATAAG GCCCAAAGAG AAGCTCCCTG GCCAAAGCAA 
GGAGGGACTG GAAGGGTGTG CTGTCAAAAT CCACCAATCT TGAAACGCCA TCAAAGGGAG 
ATAACCCTTA CTACTTTTCA GCCAGAGCCA GACAAAACTG ACTATGATGA TACTTTGTCA 
ATTGAAACAA AGAGAGAAGA TTTTGACATT TATGGTGAAG ATGAAAATCA GGACCCCCGC 
AGCTTTCAAA AGAGAACACG CCACTATTTT ATTGCTGCAG TGGAGCGGCT CTGGGATTAT 
GGGATGAGTA GATCCCCCTA CGCACTAAGA AACAGGTCTC AGAATGGAGG AGTCCCTCAG 
TTCAAAAAGG TGGTATTCGA GGAATTTACT GATGGCTCCT TTACTCAGGC CATATACCGT 
GGACAACTAA ATGAACACCT GGGACTCTTG GGACCATATA TAAGAGCAGA AGTGGAAGAC 
AATATCATGG TAACTTTCAA AAACCAGGCC TCTCGTCCCT ACTCCTTCTA TTCTAGCCTT 
ATTTCTTATA ACGGAGATCA GAGACAAGGA GCAGAACCTC GAAAAAAGTT TGTCAAGCCT 
AATGAAACCC AAAGCTACTT TTGGAAAGTG CAGCACCATA TGGCACCCAC CAAAGATGAG 
TTTGACTGCA AAGCCTGGGC TTACTTTTCT GACGTTGATC TGGAAAAAGA TTTGCACTCA 
GGCTTGATTG GCCCCATTCT GATCTGCCGC GTGGACACGC TGAGTGCTGC TCATGGGAGA 
CAAGTAACAA TACAGGAATT CGCTCTGTTT TTCACCATTT TTGATGAAAC CAAGAGCTGG 
TACTTTGCCG AAAACATGGC AAGGAACTGC GGGGCGCCCT GCCATGTCCA GCCAGAGGAC 
CCTACTTTTC AAGAAAAGTA TCGCTTCCAT GCAATCAATG GCTACGTGAT GGATACACTC 
CCTGGCTTAG TCATGGCTCA GCATCAAAGG ATTAGGTGGT ATCTGCTCAG CATGGGCAGC 
AATGAAAATA TCCATTCCAT TCATTTCAGT GGCCATGTGT TCACTGTGCG AAAAACGGAG 
GAGTATAAAA TGGCGGTCTA CAATCTCTAC CCAGGTGTCT TTGAGACGGT GGAAATGCTA 
CCATCCAAGG TTGGGACTTG GCGGATAGAA TGTCTTATTG GCGAGCACCT ACAAGCTGGG 
ATGAGCACTC TCTTCCTGGT GTACAGCAAG GAGTGTCAAA TTCCACTGGG AATGGCTTCT 
GGACGCATTA GAGATTTTCA GATTACAGCT TCAGGACAAT ATGGACAGTG GGCCCCCAAG 
CTGGCCAGAC TTCATTATTC TGGATCAATC AACGCCTGGA GCACCAAGGA TCCCTCTCCT 
TGGATCAAGG TGGATCTGTT GGCGCCGATG ATTATTCACA GCATCCTGAC TCAGGGTGCC 
CGGCAGAAGT TCTCCAGCCT GTACATCTCT CAGTTTATCA TCATGTACAG CCTCGATGGA 
CAGCGGTGGC AGGGTTATCG GGGGAACTCC ACTGGGACCT TAATGGTGTT CTTTGGTAAT 
GTGGATTCAT CTGGAGTAAA ACACAATATT TTTAACCCTC CAATTATTGC TAGATATATC 
CGTTTACACC CAACTCATTA CAGCATCCGC AGCACTCTTC GCATGGAGTT GATGGGCTGT 
GATTTAAACA GTTGCAACAT GCCACTGGGA ATGGAGAATA AAGCTATATC AGATACACAG 
ATTACTGCCT CATCCCACTT ACGCAACATG TTTGCCACCT GGTCTCCTTC ACAAGCCCGA 
CTTAACCTTC AAGGGAGGAC AAATGCCTGG AGGCCCCAGG TGAATAATCC AAAAGAGTGG 
CTACAAGTGG ACTTCCAGAA GACAATGAGA GTTACAGGAA TAACTACCCA AGGGGTGAAA 
TCTCTGCTTA CCAGCATGTA TGTGAAGGAG TTCCTCATAT CCAGTAGTCA AGATGGCCAT 
AACTGGACTC CATTTCTTCA GAATGGCAAA GTGAAGGTTT TTCAGGGAAA TCAAGACTCC 
TTCACCCCCG TGGTGAATGC TCTAGACCCC CCGCTGTTTA CCCGCTTCCT TCGGATTCAC 
CCGCGGAGCT GGGCGCACCA TATCGCCCTG AGGCTGGAGC TTTTGGGCTG CGAGGCACAG 
CAGCAGTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A mild form of haemophilia A is associated with two factor VIII missense mutations in German Fleckvieh cattle.
Animal genetics49(4)350-351(2018 Aug)
Reinartz S,Weiß C,Mischke R,Distl O

Importance of a factor VIIIc-like glycoprotein expressed in capillary endothelial cells (eFactor VIIIc) in angiogenesis.
Advances in experimental medicine and biology705453-64(2011)
Banerjee DK,Oliveira CM,Tavárez JJ,Katiyar VN,Saha S,Martínez JA,Banerjee A,Sánchez A,Baksi K

A missense mutation (p.Leu2153His) of the factor VIII gene causes cattle haemophilia A.
Animal genetics40(5)763-5(2009 Oct)
Khalaj M,Abbasi AR,Shimojo K,Moritomo Y,Yoneda K,Kunieda T

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F8 cDNA ORF clone, Bos taurus(cattle)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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