NIPBL cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	NIPBL
Entrez Gene ID	539566
Full Name	NIPBL, cohesin loading factor
General protein information	Preferred Names NIPBL, cohesin loading factor Names nipped-B-like protein
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005221614.3	XP_005221671.1	nipped-B-like protein isoform X2
XM_024981354.1	XP_024837122.1	nipped-B-like protein isoform X1
NM_001206584.2	NP_001193513.1	nipped-B-like protein
XM_015459095.2	XP_015314581.1	nipped-B-like protein isoform X3
XM_015459095.1	XP_015314581.1	nipped-B-like protein isoform X2
XM_005221614.2	XP_005221671.1	nipped-B-like protein isoform X1
NM_001206584.1	NP_001193513.1	nipped-B-like protein

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Nipped-B	NP_001163052.1
Macaca mulatta (Rhesus monkey)	NIPBL	XP_001096575.1
Rattus norvegicus (Norway rat)	Nipbl	XP_003749266.1
Homo sapiens (human)	NIPBL	NP_597677.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009290	XP_320088.4
Canis lupus familiaris (dog)	NIPBL	XP_005619439.1
Bos taurus (cattle)	NIPBL	NP_001193513.1
Mus musculus (house mouse)	Nipbl	NP_081983.2
Gallus gallus (chicken)	LOC427025	NP_001244277.1
Pan troglodytes (chimpanzee)	NIPBL	XP_001146090.1
Gallus gallus (chicken)	LOC427439	XP_425012.3
Danio rerio (zebrafish)	nipblb	NP_001154919.2
Xenopus tropicalis (tropical clawed frog)	nipbl	XP_002938229.2

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Marçais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NIPBL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NIPBL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa94122	XM_015459095.2 Latest version!	Bos taurus NIPBL, cohesin loading factor (NIPBL), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa239220	NM_001206584.2 Latest version!	Bos taurus NIPBL cohesin loading factor (NIPBL), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OBa40744	NM_001206584.1 Latest version!	Bos taurus NIPBL, cohesin loading factor (NIPBL), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OBa228057	XM_024981354.1 Latest version!	Bos taurus NIPBL, cohesin loading factor (NIPBL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OBa228058	XM_005221614.3 Latest version!	Bos taurus NIPBL, cohesin loading factor (NIPBL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OBa30682	XM_005221614.2 Latest version!	Bos taurus Nipped-B homolog (Drosophila) (NIPBL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
OBa94122	XM_015459095.1	Bos taurus Nipped-B homolog (Drosophila) (NIPBL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NIPBL ( XM_015459095.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314581.1 Bos taurus NIPBL, cohesin loading factor (NIPBL), transcript variant X3, mRNA.

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CloneID	OBa94122
Clone ID Related Accession (Same CDS sequence)	XM_015459095.2 , XM_015459095.1
Accession Version	XM_015459095.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	nipped-B-like protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015459095.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGGG ATATGCCCCA TGTCCCCATC ACTACTCTTG CGGGGATTGC TAGTCTTACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCC CCTTTACCTG CTACAACTAC AAAGAGCCTT 
CTCTTTAATG CACGAATAGC AGAAGAAGTG AACTGCCTTT TGGCCTGTAG GGATGATAAT 
TTGGTTTCAC AGCTTGTCCA CAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA 
GATAACCTTG GCAGTGATGA TCCAGAAGGC GACATACCAG TCTTGTTGCA GGCCGTCCTG 
GCGAGGAGTC CTAACGTTTT CAGGGAGAAA AGTATGCAGA ACAGATATGT ACAAAGTGGA 
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC 
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA 
AGCTCTGGGA ACAGATTTAT GCCACAACAA AATAGCCCAG TGCCTAGTCC ATATGCCCCA 
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTATACAAC ACATCCACAG 
ATGCAGCAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CCTACATGAT 
AATAAAGTTT CTGGTTCGTT GTCTGGCAAT TCTGCTAATC ATCATGCTGA TAATCCTAGA 
CATGGCTCAA GTGACGACTA CCTACACATG GTGCACAGGA TAAGTAGTGA CGATGGAGAT 
TCTTCAACAA TGAGGAATGC TGCGTCTTTT CCCTTGAGGT CTCCACAGCC AGTATGTTCC 
CCTGCTGGAA GTGATGGGAC TCCAAAAGGA TCAAGACCAT CTTTAATCCT GCAGTCTCAG 
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGACA TCTTGTTAGA TTCTCCAGAA 
AGAAAGCAAA AGAAGCAAAA GAAAATGAAA TTAGGCAAAG ATGAAAAAGA CCAGAATGAG 
AAGGCTGCAA TGTATGATAT CATTAGCTCT CCATCCAAGG ACTCCACTAA ACTTACGTTA 
AGACTTACTC GTGTAAGATC TTCAGACATG GACCAGCAAG AGGATATGCT TTCTGGTATG 
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT 
TCAAAAACAC CCATTACTCC ACAGGATGTA AACCGCCCAC TAAATGCTGC TCAGTGTTTG 
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC 
ACTTCAGTTG CTACAAAACA GCCCCAGACT TCTGTGGTAC TGAATCAGCA ACAGGTATCA 
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA 
GAGAGAGAAT CAGCTATTGA AAGGGAGCGT TTTTCAAAAG AAGTTCAAGA TAAAGGTAGT 
ATAGATCAAT CAGTGTTAAA AGAATTACCC CCTGAACTCC TGGCAGAAAT TGAATCCACC 
ATGCCACTTT GTGAACGTGT GAAAATGAAT AAACGCAAGC GTAGCACAGT TAATGAAAAA 
CCAAAATATG CTGAAATCAG TTCAGATGAA GATAATGATA GTGATGAAAC TTTTGAATCC 
TCTAGGAAAC GACATAAAAA AGATGATGAT AAAGCTTGGG AATATGAAGA GCGTGACAGA 
AGAAGCTCTG GGGATCATAG GAGAAGTGGC CACTCTCATG AAGGAAGAAG AAGTTCAGGT 
GGTGGTCGTT ATCGAAACCG AAGCCCATCA GATTCTGACA TGGAAGATTA TTCTCCTCCT 
CCCAGCCTTA GTGAGGTCGC TAGAAAAATG AAGAAAAAAG AAAAACAGAA GAAGCGGAAA 
GCTTATGAAC CAAAACTAAC ACCCGAAGAA ATGATGGATT CTTCAACTTT TAAGAGATTC 
ACAGCCTCAA TAGAGAATAT TTTGGATAAC TTAGAAGATA TGGATTTTAC TGCATTTGGT 
GATGATGATG AAATTCCTCA GGAACTGCTC TTAGGAAAAC ATCAGCTTAA TGAACTTGGC 
AGTGAATCTG CTAAAATTAA AGCAATGGGT ATAATGGATA AGCTTTCCAC TGACAAAACT 
GTGAAAGTCC TAAATATCTT GGAAAAGAAT ATTCAAGATG GGTCAAAGCT CTCCACTTTG 
TTAAATCATA ATAATGATAC TGAAGAAGAA GAAAGGTTAT GGAGAGACCT TATTATGGAG 
AGAGTGACAA AATCAGCAGA TGCCTGTCTT ACAACTATAA ACATCATGAC ATCCCCTAAC 
ATGCCAAAAG CTGTATATAT TGAGGATGTA ATCGAAAGAG TTATACAGTA CACTAAATTT 
CATTTGCAGA ATACACTTTA TCCTCAGTAT GATCCTGTTT ACAGATTAGA TCCTCATGGA 
GGAGGCTTAC TAAGTTCAAA GGCAAAACGG GCTAAATGTT CTACCCATAA GCAGAGAGTA 
ATAGTAATGC TTTATAATAA AGTTTGTGAC ATTGTTAGCA GCTTATCAGA ACTACTAGAA 
ATACAACTTC TAACAGACAC AACAATCCTT CAGGTATCTT CAATGGGAAT AACACCATTT 
TTTGTGGAAA ATGTCAGTGA ACTACAATTG TGTGCCATTA AATTAGTTAC TGCAGTATTC 
TCAAGATATG AAAAACATAG GCAGTTAATT TTGGAAGAAA TTTTTACTTC ACTGGCAAGA 
TTACCTACCA GCAAGAGGAG CTTAAGGAAC TTCAGGTTAA ATAGTAGTGA TATGGATGGA 
GAGCCTATGT ATATTCAGAT GGTTACAGCA CTGGTTTTAC AACTAATTCA GTGTGTAGTA 
CACTTACCAT CATCAGAGAA GGACTCTAAT TCAGAAGAGG ACTCAAATAA AAAAGTTGAT 
CAAGATGTTA TCATTACCAA TTCTTATGAA ACAGCCATGC GAACAGCCCA AAACTTCCTT 
TCCATCTTCC TTAAAAAATG TGGTAGTAAG CAAGGTGAAG AAGATTACAG ACCACTATTT 
GAAAATTTTG TTCAAGACCT TCTTTCAACA GTCAATAAGC CTGAATGGCC AGCTGCTGAA 
CTACTCCTTA GTTTGTTAGG GAGACTGTTG GTTCATCAGT TCAGTAATAA GTCAACAGAG 
ATGGCTTTAA GAGTGGCATC CCTTGATTAC CTTGGAACTG TCGCTGCACG ACTGAGGAAA 
GATGCTGTTA CAAGCAAAAT GGATCAAGGG TCTATAGAAC GAATTTTAAA ACAGGTTTCA 
GGAGGGGAAG ATGAAATACA GCAATTACAA AAAGCATTGC TTGATTACTT GGATGAAAAC 
ACGGAGACTG ATCCTTCACT GGTGTTTTCT CGTAAATTCT ATATTGCCCA GTGGTTCCGT 
GATACAACAC TGGAAACAGA AAAAGCAATG AAATCACAAA AAGATGAAGA ATCATCTGAA 
GGATCACATC ATGCAAAGGA AGTTGAGACA ACTGGTCAAA TTATGCATCG AGCTGAAAAC 
CGGAAGAAGT TTCTTAGAAG CATTATCAAA ACCACACCTT CTCAGTTCAG CACATTAAAA 
ATGAACTCTG ATACTGTGGA CTATGATGAT GCTTGTTTGA TTGTTCGATA CTTGGCCTCT 
ATGAGGCCGT TTGCCCAGAG CTTTGATATT TATTTGACAC AGATCCTTCG AGTCCTTGGT 
GAAAATGCAA TTGCCGTTCG AACAAAAGCC ATGAAGTGTT TATCTGAGGT TGTTGCTGTA 
GACCCCAGTA TTCTAGCAAG GCTGGATATG CAGCGAGGAG TTCATGGACG ATTAATGGAT 
AACTCCACTA GTGTCAGAGA AGCAGCAGTG GAATTACTAG GTCGATTTGT CCTTTGTCGA 
CCTCAGCTTG CTGAGCAGTA TTATGATATG CTGATTGAAA GAATATTGGA TACTGGTATC 
AGTGTCAGAA AAAGGGTGAT AAAGATTCTA AGAGACATCT GTATTGAACA ACCGACATTT 
CCAAAAATTA CAGAAATGTG TGTAAAAATG ATTCGCAGGG TAAATGATGA AGAGGGCATT 
AAGAAATTAG TAAATGAAAC ATTCCAAAAA CTCTGGTTTA CTCCAACTCC ACACAATGAC 
AAAGAAGCAA TGACAAGGAA GATTTTAAAT ATCACTGATG TGGTTGCAGC ATGCAGAGAT 
ACTGGATATG ACTGGTTTGA GCAGCTTCTT CAAAATTTGT TGAAGTCTGA AGAGGATTCC 
TCATATAAAC CTGTGAAGAA AGCTTGTACT CAACTTGTTG ATAACCTAGT TGAGCACATT 
CTTAAATATG AGGAATCTCT AGCTGACTCT GACAATAAAG GTGTGAATTC TGGAAGATTG 
GTAGCTTGCA TAACCACTTT GTTCTTATTC AGCAAAATAA GACCCCAGCT CATGGTTAAA 
CATGCCATGA CTATGCAGCC ATATCTTACC ACTAAATGTA GTACACAAAA TGATTTCATG 
GTTATCTGCA ATGTTGCAAA AATTCTAGAA CTAGTTGTAC CACTGATGGA ACATCCGAGT 
GAAACTTTCC TTGCCACTAT TGAAGAAGAT CTAATGAAGC TTATCATCAA ATATGGCATG 
ACTGTAGTGC AACATTGTGT AAGCTGTCTT GGGGCTGTTG TAAATAAAGT GACACAAAAT 
TTTAAATTTG TGTGGGCCTG TTTCAATAGA TACTATGGTG CCATTTCAAA ATTAAAAAGT 
CAACACCAAG AGGACCCAAA TAACACTTCA CTGCTAACAA ACAAACCAGC ACTTTTGAGA 
TCCCTATTCA CTGTTGGGGC ACTGTGTCGG CATTTTGATT TTGATCTGGA AGATTTTAAA 
GGCAACAGTA AGGTTAACAT AAAGGATAAA GTACTTGAAC TATTGATGTA TTTTACAAAG 
CATTCAGATG AAGAAGTGCA AACAAAAGCC ATCATTGGTC TAGGATTTGC CTTTATTCAG 
CACCCAAGTC TAATGTTTGA GCAAGAGGTG AAAAATCTAT ACAATAATAT TTTATCTGAT 
AAAAACTCCT CAGTAAATTT AAAAATACAA GTGTTAAAAA ACCTCCAGAC CTACCTACAA 
GAAGAAGATA CACGTATGCA GCAGGCAGAT AGAGACTGGA AGAAGGTTGC AAAACAGGAA 
GATTTGAAAG AAATGGGTGA TGTTTCCTCA GGGATGAGTA GTTCCATCAT GCAGCTTTAC 
CTCAAACAGG TGCTTGAGGC GTTTTTTCAC ACCCAATCAA GTGTACGCCA CTTCGCCCTC 
AATGTCATTG CATTGACTCT AAACCAAGGT CTTATTCATC CAGTTCAGTG TGTGCCGTAT 
TTAATTGCTA TGGGCACAGA CCCAGAACCA GCTATGCGGA ACAAGGCTGA TCAGCAGCTT 
GTGGAAATAG ACAAAAAATA TGCTGGATTC ATTCACATGA AAGCAGTGGC TGGCATGAAG 
ATGTCTTACC AGGTACAACA GGCAATCAAC ACGTGCCTAA AAGATCCTGT AAGGGGTTTC 
CGGCAGGACG AGTCCTCCAG TGCTTTGTGT TCACACCTTT ACTCCATGAT CCGTGGAAAC 
CGCCAGCACA GACGCGCCTT TCTGATTTCT TTACTCAACC TCTTTGATGA CACAGCAAAA 
ACAGAAGTGA ATATGCTCTT GTATATAGCA GACAATCTAG CCTGTTTTCC ATACCAGACA 
CAGGAAGAGC CGTTGTTTAT AATGCATCAT ATAGACATTA CACTCTCTGT TTCTGGTAGT 
AACCTACTGC AGTCATTCAA GGAGTCCATG GTAAAAGACA AAAGGAAAGA GAGAAAATCA 
TCTCCTGGTA AGGAAAATGA GTCAAGCGAG AGTGAAGAAG AAGTCTCTAG GCCTCGAAAG 
TCACGGAAAC GTGTAGATTC AGATTCAGAT TCGGATTCAG AAGATGATAT AAATTCGGTG 
ATGAAATGTT TGCCAGAAAA TTCAGCTCCT TTAATTGAGT TTGCAAATGT CTCCCAGGGT 
ATTTTATTGC TTCTGATGTT AAAGCAACAT TTGAAGAATC TCTGTGGATT TTCTGATAGT 
AAAATTCAGA AATACTCTCC ATCCGAATCT GCAAAAGTGT ATGATAAAGC GATAAACCGG 
AAAACAGGAG TTCATTTTCA TCCCAAGCAA ACACTGGACT TCCTGCGGAG TGATATGGCT 
AATTCCAAAA TCACTGAAGA TGTGAAGAGG AGTATAGTAA AACAGTACCT AGATTTCAAA 
CTTCTCATGG AACATCTGGA CCCTGATGAA GAGGAAGAAG AAGGGGAGGT GTCAGCCAGC 
ACAAATGCTC GGAACAAAGC AATTACCTCG CTGCTTGGAG GAGGCAGCCC TAAAAATAAT 
GCAGCAGCAG AGACAGAAGA TGATGAAAGT GATGGGGAGG ATAGAGGAGG AGGCACTTCA 
GGGTCATTGA GAAGGTCAAA ACGAAATTCA GACTCTACGG AGTTGGCAGC ACAGATGAAT 
GAAAGTGTTG ACGTCATGGA TGTCATCGCT ATTTGCTGTC CAAAGTACAA AGACCGACCA 
CAGATTGCAA GAGTAGTACA GAAAACCAGC AGTGGCTTCA GTGTTCAGTG GATGGCAGGC 
TCCTACAGTG GCTCCTGGAC TGAGGCTAAG CGCCGTGATG GCCGCAAACT GGTGCCTTGG 
GTAGACACTA TTAAAGAGTC AGACATTATT TACAAAAAAA TTGCTCTAAC GAGTGCTAAT 
AAGCTGACTA ATAAAGTTGT TCAGACTTTA CGATCCCTGT ATGCCGCCAA GGATGGGACT 
TCCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314581.1
CDS	500..7288
Translation	MNGDMPHVPITTLAGIASLTDLLNQLPLPSPLPATTTKSLLFNARIAEEVNCLLACRDDNLVSQLVHSLNQVSTDHIELKDNLGSDDPEGDIPVLLQAVLARSPNVFREKSMQNRYVQSGMMMSQYKLSQNSMHSSPASSNYQQTTISHSPSSRFVPPQTSSGNRFMPQQNSPVPSPYAPQSPAGYMPYSHPSSYTTHPQMQQASVSSPIVAGGLRNLHDNKVSGSLSGNSANHHADNPRHGSSDDYLHMVHRISSDDGDSSTMRNAASFPLRSPQPVCSPAGSDGTPKGSRPSLILQSQSLPCSSPRDVPPDILLDSPERKQKKQKKMKLGKDEKDQNEKAAMYDIISSPSKDSTKLTLRLTRVRSSDMDQQEDMLSGMENSNVSENDIPFNVQYPGQTSKTPITPQDVNRPLNAAQCLSQQEQTAFLPANQVPVLQQNTSVATKQPQTSVVLNQQQVSQQGPIYDEVELDALAEIERIERESAIERERFSKEVQDKGSIDQSVLKELPPELLAEIESTMPLCERVKMNKRKRSTVNEKPKYAEISSDEDNDSDETFESSRKRHKKDDDKAWEYEERDRRSSGDHRRSGHSHEGRRSSGGGRYRNRSPSDSDMEDYSPPPSLSEVARKMKKKEKQKKRKAYEPKLTPEEMMDSSTFKRFTASIENILDNLEDMDFTAFGDDDEIPQELLLGKHQLNELGSESAKIKAMGIMDKLSTDKTVKVLNILEKNIQDGSKLSTLLNHNNDTEEEERLWRDLIMERVTKSADACLTTINIMTSPNMPKAVYIEDVIERVIQYTKFHLQNTLYPQYDPVYRLDPHGGGLLSSKAKRAKCSTHKQRVIVMLYNKVCDIVSSLSELLEIQLLTDTTILQVSSMGITPFFVENVSELQLCAIKLVTAVFSRYEKHRQLILEEIFTSLARLPTSKRSLRNFRLNSSDMDGEPMYIQMVTALVLQLIQCVVHLPSSEKDSNSEEDSNKKVDQDVIITNSYETAMRTAQNFLSIFLKKCGSKQGEEDYRPLFENFVQDLLSTVNKPEWPAAELLLSLLGRLLVHQFSNKSTEMALRVASLDYLGTVAARLRKDAVTSKMDQGSIERILKQVSGGEDEIQQLQKALLDYLDENTETDPSLVFSRKFYIAQWFRDTTLETEKAMKSQKDEESSEGSHHAKEVETTGQIMHRAENRKKFLRSIIKTTPSQFSTLKMNSDTVDYDDACLIVRYLASMRPFAQSFDIYLTQILRVLGENAIAVRTKAMKCLSEVVAVDPSILARLDMQRGVHGRLMDNSTSVREAAVELLGRFVLCRPQLAEQYYDMLIERILDTGISVRKRVIKILRDICIEQPTFPKITEMCVKMIRRVNDEEGIKKLVNETFQKLWFTPTPHNDKEAMTRKILNITDVVAACRDTGYDWFEQLLQNLLKSEEDSSYKPVKKACTQLVDNLVEHILKYEESLADSDNKGVNSGRLVACITTLFLFSKIRPQLMVKHAMTMQPYLTTKCSTQNDFMVICNVAKILELVVPLMEHPSETFLATIEEDLMKLIIKYGMTVVQHCVSCLGAVVNKVTQNFKFVWACFNRYYGAISKLKSQHQEDPNNTSLLTNKPALLRSLFTVGALCRHFDFDLEDFKGNSKVNIKDKVLELLMYFTKHSDEEVQTKAIIGLGFAFIQHPSLMFEQEVKNLYNNILSDKNSSVNLKIQVLKNLQTYLQEEDTRMQQADRDWKKVAKQEDLKEMGDVSSGMSSSIMQLYLKQVLEAFFHTQSSVRHFALNVIALTLNQGLIHPVQCVPYLIAMGTDPEPAMRNKADQQLVEIDKKYAGFIHMKAVAGMKMSYQVQQAINTCLKDPVRGFRQDESSSALCSHLYSMIRGNRQHRRAFLISLLNLFDDTAKTEVNMLLYIADNLACFPYQTQEEPLFIMHHIDITLSVSGSNLLQSFKESMVKDKRKERKSSPGKENESSESEEEVSRPRKSRKRVDSDSDSDSEDDINSVMKCLPENSAPLIEFANVSQGILLLLMLKQHLKNLCGFSDSKIQKYSPSESAKVYDKAINRKTGVHFHPKQTLDFLRSDMANSKITEDVKRSIVKQYLDFKLLMEHLDPDEEEEEGEVSASTNARNKAITSLLGGGSPKNNAAAETEDDESDGEDRGGGTSGSLRRSKRNSDSTELAAQMNESVDVMDVIAICCPKYKDRPQIARVVQKTSSGFSVQWMAGSYSGSWTEAKRRDGRKLVPWVDTIKESDIIYKKIALTSANKLTNKVVQTLRSLYAAKDGTSS

Target ORF information:

RefSeq Version	XM_015459095.2
Organism	Bos taurus(cattle)
Definition	Bos taurus NIPBL, cohesin loading factor (NIPBL), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015459095.2

ORF Insert Sequence:

ATGAATGGGG ATATGCCCCA TGTCCCCATC ACTACTCTTG CGGGGATTGC TAGTCTTACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCC CCTTTACCTG CTACAACTAC AAAGAGCCTT 
CTCTTTAATG CACGAATAGC AGAAGAAGTG AACTGCCTTT TGGCCTGTAG GGATGATAAT 
TTGGTTTCAC AGCTTGTCCA CAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA 
GATAACCTTG GCAGTGATGA TCCAGAAGGC GACATACCAG TCTTGTTGCA GGCCGTCCTG 
GCGAGGAGTC CTAACGTTTT CAGGGAGAAA AGTATGCAGA ACAGATATGT ACAAAGTGGA 
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC 
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA 
AGCTCTGGGA ACAGATTTAT GCCACAACAA AATAGCCCAG TGCCTAGTCC ATATGCCCCA 
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTATACAAC ACATCCACAG 
ATGCAGCAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CCTACATGAT 
AATAAAGTTT CTGGTTCGTT GTCTGGCAAT TCTGCTAATC ATCATGCTGA TAATCCTAGA 
CATGGCTCAA GTGACGACTA CCTACACATG GTGCACAGGA TAAGTAGTGA CGATGGAGAT 
TCTTCAACAA TGAGGAATGC TGCGTCTTTT CCCTTGAGGT CTCCACAGCC AGTATGTTCC 
CCTGCTGGAA GTGATGGGAC TCCAAAAGGA TCAAGACCAT CTTTAATCCT GCAGTCTCAG 
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGACA TCTTGTTAGA TTCTCCAGAA 
AGAAAGCAAA AGAAGCAAAA GAAAATGAAA TTAGGCAAAG ATGAAAAAGA CCAGAATGAG 
AAGGCTGCAA TGTATGATAT CATTAGCTCT CCATCCAAGG ACTCCACTAA ACTTACGTTA 
AGACTTACTC GTGTAAGATC TTCAGACATG GACCAGCAAG AGGATATGCT TTCTGGTATG 
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT 
TCAAAAACAC CCATTACTCC ACAGGATGTA AACCGCCCAC TAAATGCTGC TCAGTGTTTG 
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC 
ACTTCAGTTG CTACAAAACA GCCCCAGACT TCTGTGGTAC TGAATCAGCA ACAGGTATCA 
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA 
GAGAGAGAAT CAGCTATTGA AAGGGAGCGT TTTTCAAAAG AAGTTCAAGA TAAAGGTAGT 
ATAGATCAAT CAGTGTTAAA AGAATTACCC CCTGAACTCC TGGCAGAAAT TGAATCCACC 
ATGCCACTTT GTGAACGTGT GAAAATGAAT AAACGCAAGC GTAGCACAGT TAATGAAAAA 
CCAAAATATG CTGAAATCAG TTCAGATGAA GATAATGATA GTGATGAAAC TTTTGAATCC 
TCTAGGAAAC GACATAAAAA AGATGATGAT AAAGCTTGGG AATATGAAGA GCGTGACAGA 
AGAAGCTCTG GGGATCATAG GAGAAGTGGC CACTCTCATG AAGGAAGAAG AAGTTCAGGT 
GGTGGTCGTT ATCGAAACCG AAGCCCATCA GATTCTGACA TGGAAGATTA TTCTCCTCCT 
CCCAGCCTTA GTGAGGTCGC TAGAAAAATG AAGAAAAAAG AAAAACAGAA GAAGCGGAAA 
GCTTATGAAC CAAAACTAAC ACCCGAAGAA ATGATGGATT CTTCAACTTT TAAGAGATTC 
ACAGCCTCAA TAGAGAATAT TTTGGATAAC TTAGAAGATA TGGATTTTAC TGCATTTGGT 
GATGATGATG AAATTCCTCA GGAACTGCTC TTAGGAAAAC ATCAGCTTAA TGAACTTGGC 
AGTGAATCTG CTAAAATTAA AGCAATGGGT ATAATGGATA AGCTTTCCAC TGACAAAACT 
GTGAAAGTCC TAAATATCTT GGAAAAGAAT ATTCAAGATG GGTCAAAGCT CTCCACTTTG 
TTAAATCATA ATAATGATAC TGAAGAAGAA GAAAGGTTAT GGAGAGACCT TATTATGGAG 
AGAGTGACAA AATCAGCAGA TGCCTGTCTT ACAACTATAA ACATCATGAC ATCCCCTAAC 
ATGCCAAAAG CTGTATATAT TGAGGATGTA ATCGAAAGAG TTATACAGTA CACTAAATTT 
CATTTGCAGA ATACACTTTA TCCTCAGTAT GATCCTGTTT ACAGATTAGA TCCTCATGGA 
GGAGGCTTAC TAAGTTCAAA GGCAAAACGG GCTAAATGTT CTACCCATAA GCAGAGAGTA 
ATAGTAATGC TTTATAATAA AGTTTGTGAC ATTGTTAGCA GCTTATCAGA ACTACTAGAA 
ATACAACTTC TAACAGACAC AACAATCCTT CAGGTATCTT CAATGGGAAT AACACCATTT 
TTTGTGGAAA ATGTCAGTGA ACTACAATTG TGTGCCATTA AATTAGTTAC TGCAGTATTC 
TCAAGATATG AAAAACATAG GCAGTTAATT TTGGAAGAAA TTTTTACTTC ACTGGCAAGA 
TTACCTACCA GCAAGAGGAG CTTAAGGAAC TTCAGGTTAA ATAGTAGTGA TATGGATGGA 
GAGCCTATGT ATATTCAGAT GGTTACAGCA CTGGTTTTAC AACTAATTCA GTGTGTAGTA 
CACTTACCAT CATCAGAGAA GGACTCTAAT TCAGAAGAGG ACTCAAATAA AAAAGTTGAT 
CAAGATGTTA TCATTACCAA TTCTTATGAA ACAGCCATGC GAACAGCCCA AAACTTCCTT 
TCCATCTTCC TTAAAAAATG TGGTAGTAAG CAAGGTGAAG AAGATTACAG ACCACTATTT 
GAAAATTTTG TTCAAGACCT TCTTTCAACA GTCAATAAGC CTGAATGGCC AGCTGCTGAA 
CTACTCCTTA GTTTGTTAGG GAGACTGTTG GTTCATCAGT TCAGTAATAA GTCAACAGAG 
ATGGCTTTAA GAGTGGCATC CCTTGATTAC CTTGGAACTG TCGCTGCACG ACTGAGGAAA 
GATGCTGTTA CAAGCAAAAT GGATCAAGGG TCTATAGAAC GAATTTTAAA ACAGGTTTCA 
GGAGGGGAAG ATGAAATACA GCAATTACAA AAAGCATTGC TTGATTACTT GGATGAAAAC 
ACGGAGACTG ATCCTTCACT GGTGTTTTCT CGTAAATTCT ATATTGCCCA GTGGTTCCGT 
GATACAACAC TGGAAACAGA AAAAGCAATG AAATCACAAA AAGATGAAGA ATCATCTGAA 
GGATCACATC ATGCAAAGGA AGTTGAGACA ACTGGTCAAA TTATGCATCG AGCTGAAAAC 
CGGAAGAAGT TTCTTAGAAG CATTATCAAA ACCACACCTT CTCAGTTCAG CACATTAAAA 
ATGAACTCTG ATACTGTGGA CTATGATGAT GCTTGTTTGA TTGTTCGATA CTTGGCCTCT 
ATGAGGCCGT TTGCCCAGAG CTTTGATATT TATTTGACAC AGATCCTTCG AGTCCTTGGT 
GAAAATGCAA TTGCCGTTCG AACAAAAGCC ATGAAGTGTT TATCTGAGGT TGTTGCTGTA 
GACCCCAGTA TTCTAGCAAG GCTGGATATG CAGCGAGGAG TTCATGGACG ATTAATGGAT 
AACTCCACTA GTGTCAGAGA AGCAGCAGTG GAATTACTAG GTCGATTTGT CCTTTGTCGA 
CCTCAGCTTG CTGAGCAGTA TTATGATATG CTGATTGAAA GAATATTGGA TACTGGTATC 
AGTGTCAGAA AAAGGGTGAT AAAGATTCTA AGAGACATCT GTATTGAACA ACCGACATTT 
CCAAAAATTA CAGAAATGTG TGTAAAAATG ATTCGCAGGG TAAATGATGA AGAGGGCATT 
AAGAAATTAG TAAATGAAAC ATTCCAAAAA CTCTGGTTTA CTCCAACTCC ACACAATGAC 
AAAGAAGCAA TGACAAGGAA GATTTTAAAT ATCACTGATG TGGTTGCAGC ATGCAGAGAT 
ACTGGATATG ACTGGTTTGA GCAGCTTCTT CAAAATTTGT TGAAGTCTGA AGAGGATTCC 
TCATATAAAC CTGTGAAGAA AGCTTGTACT CAACTTGTTG ATAACCTAGT TGAGCACATT 
CTTAAATATG AGGAATCTCT AGCTGACTCT GACAATAAAG GTGTGAATTC TGGAAGATTG 
GTAGCTTGCA TAACCACTTT GTTCTTATTC AGCAAAATAA GACCCCAGCT CATGGTTAAA 
CATGCCATGA CTATGCAGCC ATATCTTACC ACTAAATGTA GTACACAAAA TGATTTCATG 
GTTATCTGCA ATGTTGCAAA AATTCTAGAA CTAGTTGTAC CACTGATGGA ACATCCGAGT 
GAAACTTTCC TTGCCACTAT TGAAGAAGAT CTAATGAAGC TTATCATCAA ATATGGCATG 
ACTGTAGTGC AACATTGTGT AAGCTGTCTT GGGGCTGTTG TAAATAAAGT GACACAAAAT 
TTTAAATTTG TGTGGGCCTG TTTCAATAGA TACTATGGTG CCATTTCAAA ATTAAAAAGT 
CAACACCAAG AGGACCCAAA TAACACTTCA CTGCTAACAA ACAAACCAGC ACTTTTGAGA 
TCCCTATTCA CTGTTGGGGC ACTGTGTCGG CATTTTGATT TTGATCTGGA AGATTTTAAA 
GGCAACAGTA AGGTTAACAT AAAGGATAAA GTACTTGAAC TATTGATGTA TTTTACAAAG 
CATTCAGATG AAGAAGTGCA AACAAAAGCC ATCATTGGTC TAGGATTTGC CTTTATTCAG 
CACCCAAGTC TAATGTTTGA GCAAGAGGTG AAAAATCTAT ACAATAATAT TTTATCTGAT 
AAAAACTCCT CAGTAAATTT AAAAATACAA GTGTTAAAAA ACCTCCAGAC CTACCTACAA 
GAAGAAGATA CACGTATGCA GCAGGCAGAT AGAGACTGGA AGAAGGTTGC AAAACAGGAA 
GATTTGAAAG AAATGGGTGA TGTTTCCTCA GGGATGAGTA GTTCCATCAT GCAGCTTTAC 
CTCAAACAGG TGCTTGAGGC GTTTTTTCAC ACCCAATCAA GTGTACGCCA CTTCGCCCTC 
AATGTCATTG CATTGACTCT AAACCAAGGT CTTATTCATC CAGTTCAGTG TGTGCCGTAT 
TTAATTGCTA TGGGCACAGA CCCAGAACCA GCTATGCGGA ACAAGGCTGA TCAGCAGCTT 
GTGGAAATAG ACAAAAAATA TGCTGGATTC ATTCACATGA AAGCAGTGGC TGGCATGAAG 
ATGTCTTACC AGGTACAACA GGCAATCAAC ACGTGCCTAA AAGATCCTGT AAGGGGTTTC 
CGGCAGGACG AGTCCTCCAG TGCTTTGTGT TCACACCTTT ACTCCATGAT CCGTGGAAAC 
CGCCAGCACA GACGCGCCTT TCTGATTTCT TTACTCAACC TCTTTGATGA CACAGCAAAA 
ACAGAAGTGA ATATGCTCTT GTATATAGCA GACAATCTAG CCTGTTTTCC ATACCAGACA 
CAGGAAGAGC CGTTGTTTAT AATGCATCAT ATAGACATTA CACTCTCTGT TTCTGGTAGT 
AACCTACTGC AGTCATTCAA GGAGTCCATG GTAAAAGACA AAAGGAAAGA GAGAAAATCA 
TCTCCTGGTA AGGAAAATGA GTCAAGCGAG AGTGAAGAAG AAGTCTCTAG GCCTCGAAAG 
TCACGGAAAC GTGTAGATTC AGATTCAGAT TCGGATTCAG AAGATGATAT AAATTCGGTG 
ATGAAATGTT TGCCAGAAAA TTCAGCTCCT TTAATTGAGT TTGCAAATGT CTCCCAGGGT 
ATTTTATTGC TTCTGATGTT AAAGCAACAT TTGAAGAATC TCTGTGGATT TTCTGATAGT 
AAAATTCAGA AATACTCTCC ATCCGAATCT GCAAAAGTGT ATGATAAAGC GATAAACCGG 
AAAACAGGAG TTCATTTTCA TCCCAAGCAA ACACTGGACT TCCTGCGGAG TGATATGGCT 
AATTCCAAAA TCACTGAAGA TGTGAAGAGG AGTATAGTAA AACAGTACCT AGATTTCAAA 
CTTCTCATGG AACATCTGGA CCCTGATGAA GAGGAAGAAG AAGGGGAGGT GTCAGCCAGC 
ACAAATGCTC GGAACAAAGC AATTACCTCG CTGCTTGGAG GAGGCAGCCC TAAAAATAAT 
GCAGCAGCAG AGACAGAAGA TGATGAAAGT GATGGGGAGG ATAGAGGAGG AGGCACTTCA 
GGGTCATTGA GAAGGTCAAA ACGAAATTCA GACTCTACGG AGTTGGCAGC ACAGATGAAT 
GAAAGTGTTG ACGTCATGGA TGTCATCGCT ATTTGCTGTC CAAAGTACAA AGACCGACCA 
CAGATTGCAA GAGTAGTACA GAAAACCAGC AGTGGCTTCA GTGTTCAGTG GATGGCAGGC 
TCCTACAGTG GCTCCTGGAC TGAGGCTAAG CGCCGTGATG GCCGCAAACT GGTGCCTTGG 
GTAGACACTA TTAAAGAGTC AGACATTATT TACAAAAAAA TTGCTCTAAC GAGTGCTAAT 
AAGCTGACTA ATAAAGTTGT TCAGACTTTA CGATCCCTGT ATGCCGCCAA GGATGGGACT 
TCCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIPBL ( XM_015459095.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314581.1 Bos taurus Nipped-B homolog (Drosophila) (NIPBL), transcript variant X2, mRNA.

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CloneID	OBa94122
Clone ID Related Accession (Same CDS sequence)	XM_015459095.2 , XM_015459095.1
Accession Version	XM_015459095.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	nipped-B-like protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104515.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGGG ATATGCCCCA TGTCCCCATC ACTACTCTTG CGGGGATTGC TAGTCTTACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCC CCTTTACCTG CTACAACTAC AAAGAGCCTT 
CTCTTTAATG CACGAATAGC AGAAGAAGTG AACTGCCTTT TGGCCTGTAG GGATGATAAT 
TTGGTTTCAC AGCTTGTCCA CAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA 
GATAACCTTG GCAGTGATGA TCCAGAAGGC GACATACCAG TCTTGTTGCA GGCCGTCCTG 
GCGAGGAGTC CTAACGTTTT CAGGGAGAAA AGTATGCAGA ACAGATATGT ACAAAGTGGA 
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC 
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA 
AGCTCTGGGA ACAGATTTAT GCCACAACAA AATAGCCCAG TGCCTAGTCC ATATGCCCCA 
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTATACAAC ACATCCACAG 
ATGCAGCAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CCTACATGAT 
AATAAAGTTT CTGGTTCGTT GTCTGGCAAT TCTGCTAATC ATCATGCTGA TAATCCTAGA 
CATGGCTCAA GTGACGACTA CCTACACATG GTGCACAGGA TAAGTAGTGA CGATGGAGAT 
TCTTCAACAA TGAGGAATGC TGCGTCTTTT CCCTTGAGGT CTCCACAGCC AGTATGTTCC 
CCTGCTGGAA GTGATGGGAC TCCAAAAGGA TCAAGACCAT CTTTAATCCT GCAGTCTCAG 
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGACA TCTTGTTAGA TTCTCCAGAA 
AGAAAGCAAA AGAAGCAAAA GAAAATGAAA TTAGGCAAAG ATGAAAAAGA CCAGAATGAG 
AAGGCTGCAA TGTATGATAT CATTAGCTCT CCATCCAAGG ACTCCACTAA ACTTACGTTA 
AGACTTACTC GTGTAAGATC TTCAGACATG GACCAGCAAG AGGATATGCT TTCTGGTATG 
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT 
TCAAAAACAC CCATTACTCC ACAGGATGTA AACCGCCCAC TAAATGCTGC TCAGTGTTTG 
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC 
ACTTCAGTTG CTACAAAACA GCCCCAGACT TCTGTGGTAC TGAATCAGCA ACAGGTATCA 
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA 
GAGAGAGAAT CAGCTATTGA AAGGGAGCGT TTTTCAAAAG AAGTTCAAGA TAAAGGTAGT 
ATAGATCAAT CAGTGTTAAA AGAATTACCC CCTGAACTCC TGGCAGAAAT TGAATCCACC 
ATGCCACTTT GTGAACGTGT GAAAATGAAT AAACGCAAGC GTAGCACAGT TAATGAAAAA 
CCAAAATATG CTGAAATCAG TTCAGATGAA GATAATGATA GTGATGAAAC TTTTGAATCC 
TCTAGGAAAC GACATAAAAA AGATGATGAT AAAGCTTGGG AATATGAAGA GCGTGACAGA 
AGAAGCTCTG GGGATCATAG GAGAAGTGGC CACTCTCATG AAGGAAGAAG AAGTTCAGGT 
GGTGGTCGTT ATCGAAACCG AAGCCCATCA GATTCTGACA TGGAAGATTA TTCTCCTCCT 
CCCAGCCTTA GTGAGGTCGC TAGAAAAATG AAGAAAAAAG AAAAACAGAA GAAGCGGAAA 
GCTTATGAAC CAAAACTAAC ACCCGAAGAA ATGATGGATT CTTCAACTTT TAAGAGATTC 
ACAGCCTCAA TAGAGAATAT TTTGGATAAC TTAGAAGATA TGGATTTTAC TGCATTTGGT 
GATGATGATG AAATTCCTCA GGAACTGCTC TTAGGAAAAC ATCAGCTTAA TGAACTTGGC 
AGTGAATCTG CTAAAATTAA AGCAATGGGT ATAATGGATA AGCTTTCCAC TGACAAAACT 
GTGAAAGTCC TAAATATCTT GGAAAAGAAT ATTCAAGATG GGTCAAAGCT CTCCACTTTG 
TTAAATCATA ATAATGATAC TGAAGAAGAA GAAAGGTTAT GGAGAGACCT TATTATGGAG 
AGAGTGACAA AATCAGCAGA TGCCTGTCTT ACAACTATAA ACATCATGAC ATCCCCTAAC 
ATGCCAAAAG CTGTATATAT TGAGGATGTA ATCGAAAGAG TTATACAGTA CACTAAATTT 
CATTTGCAGA ATACACTTTA TCCTCAGTAT GATCCTGTTT ACAGATTAGA TCCTCATGGA 
GGAGGCTTAC TAAGTTCAAA GGCAAAACGG GCTAAATGTT CTACCCATAA GCAGAGAGTA 
ATAGTAATGC TTTATAATAA AGTTTGTGAC ATTGTTAGCA GCTTATCAGA ACTACTAGAA 
ATACAACTTC TAACAGACAC AACAATCCTT CAGGTATCTT CAATGGGAAT AACACCATTT 
TTTGTGGAAA ATGTCAGTGA ACTACAATTG TGTGCCATTA AATTAGTTAC TGCAGTATTC 
TCAAGATATG AAAAACATAG GCAGTTAATT TTGGAAGAAA TTTTTACTTC ACTGGCAAGA 
TTACCTACCA GCAAGAGGAG CTTAAGGAAC TTCAGGTTAA ATAGTAGTGA TATGGATGGA 
GAGCCTATGT ATATTCAGAT GGTTACAGCA CTGGTTTTAC AACTAATTCA GTGTGTAGTA 
CACTTACCAT CATCAGAGAA GGACTCTAAT TCAGAAGAGG ACTCAAATAA AAAAGTTGAT 
CAAGATGTTA TCATTACCAA TTCTTATGAA ACAGCCATGC GAACAGCCCA AAACTTCCTT 
TCCATCTTCC TTAAAAAATG TGGTAGTAAG CAAGGTGAAG AAGATTACAG ACCACTATTT 
GAAAATTTTG TTCAAGACCT TCTTTCAACA GTCAATAAGC CTGAATGGCC AGCTGCTGAA 
CTACTCCTTA GTTTGTTAGG GAGACTGTTG GTTCATCAGT TCAGTAATAA GTCAACAGAG 
ATGGCTTTAA GAGTGGCATC CCTTGATTAC CTTGGAACTG TCGCTGCACG ACTGAGGAAA 
GATGCTGTTA CAAGCAAAAT GGATCAAGGG TCTATAGAAC GAATTTTAAA ACAGGTTTCA 
GGAGGGGAAG ATGAAATACA GCAATTACAA AAAGCATTGC TTGATTACTT GGATGAAAAC 
ACGGAGACTG ATCCTTCACT GGTGTTTTCT CGTAAATTCT ATATTGCCCA GTGGTTCCGT 
GATACAACAC TGGAAACAGA AAAAGCAATG AAATCACAAA AAGATGAAGA ATCATCTGAA 
GGATCACATC ATGCAAAGGA AGTTGAGACA ACTGGTCAAA TTATGCATCG AGCTGAAAAC 
CGGAAGAAGT TTCTTAGAAG CATTATCAAA ACCACACCTT CTCAGTTCAG CACATTAAAA 
ATGAACTCTG ATACTGTGGA CTATGATGAT GCTTGTTTGA TTGTTCGATA CTTGGCCTCT 
ATGAGGCCGT TTGCCCAGAG CTTTGATATT TATTTGACAC AGATCCTTCG AGTCCTTGGT 
GAAAATGCAA TTGCCGTTCG AACAAAAGCC ATGAAGTGTT TATCTGAGGT TGTTGCTGTA 
GACCCCAGTA TTCTAGCAAG GCTGGATATG CAGCGAGGAG TTCATGGACG ATTAATGGAT 
AACTCCACTA GTGTCAGAGA AGCAGCAGTG GAATTACTAG GTCGATTTGT CCTTTGTCGA 
CCTCAGCTTG CTGAGCAGTA TTATGATATG CTGATTGAAA GAATATTGGA TACTGGTATC 
AGTGTCAGAA AAAGGGTGAT AAAGATTCTA AGAGACATCT GTATTGAACA ACCGACATTT 
CCAAAAATTA CAGAAATGTG TGTAAAAATG ATTCGCAGGG TAAATGATGA AGAGGGCATT 
AAGAAATTAG TAAATGAAAC ATTCCAAAAA CTCTGGTTTA CTCCAACTCC ACACAATGAC 
AAAGAAGCAA TGACAAGGAA GATTTTAAAT ATCACTGATG TGGTTGCAGC ATGCAGAGAT 
ACTGGATATG ACTGGTTTGA GCAGCTTCTT CAAAATTTGT TGAAGTCTGA AGAGGATTCC 
TCATATAAAC CTGTGAAGAA AGCTTGTACT CAACTTGTTG ATAACCTAGT TGAGCACATT 
CTTAAATATG AGGAATCTCT AGCTGACTCT GACAATAAAG GTGTGAATTC TGGAAGATTG 
GTAGCTTGCA TAACCACTTT GTTCTTATTC AGCAAAATAA GACCCCAGCT CATGGTTAAA 
CATGCCATGA CTATGCAGCC ATATCTTACC ACTAAATGTA GTACACAAAA TGATTTCATG 
GTTATCTGCA ATGTTGCAAA AATTCTAGAA CTAGTTGTAC CACTGATGGA ACATCCGAGT 
GAAACTTTCC TTGCCACTAT TGAAGAAGAT CTAATGAAGC TTATCATCAA ATATGGCATG 
ACTGTAGTGC AACATTGTGT AAGCTGTCTT GGGGCTGTTG TAAATAAAGT GACACAAAAT 
TTTAAATTTG TGTGGGCCTG TTTCAATAGA TACTATGGTG CCATTTCAAA ATTAAAAAGT 
CAACACCAAG AGGACCCAAA TAACACTTCA CTGCTAACAA ACAAACCAGC ACTTTTGAGA 
TCCCTATTCA CTGTTGGGGC ACTGTGTCGG CATTTTGATT TTGATCTGGA AGATTTTAAA 
GGCAACAGTA AGGTTAACAT AAAGGATAAA GTACTTGAAC TATTGATGTA TTTTACAAAG 
CATTCAGATG AAGAAGTGCA AACAAAAGCC ATCATTGGTC TAGGATTTGC CTTTATTCAG 
CACCCAAGTC TAATGTTTGA GCAAGAGGTG AAAAATCTAT ACAATAATAT TTTATCTGAT 
AAAAACTCCT CAGTAAATTT AAAAATACAA GTGTTAAAAA ACCTCCAGAC CTACCTACAA 
GAAGAAGATA CACGTATGCA GCAGGCAGAT AGAGACTGGA AGAAGGTTGC AAAACAGGAA 
GATTTGAAAG AAATGGGTGA TGTTTCCTCA GGGATGAGTA GTTCCATCAT GCAGCTTTAC 
CTCAAACAGG TGCTTGAGGC GTTTTTTCAC ACCCAATCAA GTGTACGCCA CTTCGCCCTC 
AATGTCATTG CATTGACTCT AAACCAAGGT CTTATTCATC CAGTTCAGTG TGTGCCGTAT 
TTAATTGCTA TGGGCACAGA CCCAGAACCA GCTATGCGGA ACAAGGCTGA TCAGCAGCTT 
GTGGAAATAG ACAAAAAATA TGCTGGATTC ATTCACATGA AAGCAGTGGC TGGCATGAAG 
ATGTCTTACC AGGTACAACA GGCAATCAAC ACGTGCCTAA AAGATCCTGT AAGGGGTTTC 
CGGCAGGACG AGTCCTCCAG TGCTTTGTGT TCACACCTTT ACTCCATGAT CCGTGGAAAC 
CGCCAGCACA GACGCGCCTT TCTGATTTCT TTACTCAACC TCTTTGATGA CACAGCAAAA 
ACAGAAGTGA ATATGCTCTT GTATATAGCA GACAATCTAG CCTGTTTTCC ATACCAGACA 
CAGGAAGAGC CGTTGTTTAT AATGCATCAT ATAGACATTA CACTCTCTGT TTCTGGTAGT 
AACCTACTGC AGTCATTCAA GGAGTCCATG GTAAAAGACA AAAGGAAAGA GAGAAAATCA 
TCTCCTGGTA AGGAAAATGA GTCAAGCGAG AGTGAAGAAG AAGTCTCTAG GCCTCGAAAG 
TCACGGAAAC GTGTAGATTC AGATTCAGAT TCGGATTCAG AAGATGATAT AAATTCGGTG 
ATGAAATGTT TGCCAGAAAA TTCAGCTCCT TTAATTGAGT TTGCAAATGT CTCCCAGGGT 
ATTTTATTGC TTCTGATGTT AAAGCAACAT TTGAAGAATC TCTGTGGATT TTCTGATAGT 
AAAATTCAGA AATACTCTCC ATCCGAATCT GCAAAAGTGT ATGATAAAGC GATAAACCGG 
AAAACAGGAG TTCATTTTCA TCCCAAGCAA ACACTGGACT TCCTGCGGAG TGATATGGCT 
AATTCCAAAA TCACTGAAGA TGTGAAGAGG AGTATAGTAA AACAGTACCT AGATTTCAAA 
CTTCTCATGG AACATCTGGA CCCTGATGAA GAGGAAGAAG AAGGGGAGGT GTCAGCCAGC 
ACAAATGCTC GGAACAAAGC AATTACCTCG CTGCTTGGAG GAGGCAGCCC TAAAAATAAT 
GCAGCAGCAG AGACAGAAGA TGATGAAAGT GATGGGGAGG ATAGAGGAGG AGGCACTTCA 
GGGTCATTGA GAAGGTCAAA ACGAAATTCA GACTCTACGG AGTTGGCAGC ACAGATGAAT 
GAAAGTGTTG ACGTCATGGA TGTCATCGCT ATTTGCTGTC CAAAGTACAA AGACCGACCA 
CAGATTGCAA GAGTAGTACA GAAAACCAGC AGTGGCTTCA GTGTTCAGTG GATGGCAGGC 
TCCTACAGTG GCTCCTGGAC TGAGGCTAAG CGCCGTGATG GCCGCAAACT GGTGCCTTGG 
GTAGACACTA TTAAAGAGTC AGACATTATT TACAAAAAAA TTGCTCTAAC GAGTGCTAAT 
AAGCTGACTA ATAAAGTTGT TCAGACTTTA CGATCCCTGT ATGCCGCCAA GGATGGGACT 
TCCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314581.1
CDS	423..7211
Translation	MNGDMPHVPITTLAGIASLTDLLNQLPLPSPLPATTTKSLLFNARIAEEVNCLLACRDDNLVSQLVHSLNQVSTDHIELKDNLGSDDPEGDIPVLLQAVLARSPNVFREKSMQNRYVQSGMMMSQYKLSQNSMHSSPASSNYQQTTISHSPSSRFVPPQTSSGNRFMPQQNSPVPSPYAPQSPAGYMPYSHPSSYTTHPQMQQASVSSPIVAGGLRNLHDNKVSGSLSGNSANHHADNPRHGSSDDYLHMVHRISSDDGDSSTMRNAASFPLRSPQPVCSPAGSDGTPKGSRPSLILQSQSLPCSSPRDVPPDILLDSPERKQKKQKKMKLGKDEKDQNEKAAMYDIISSPSKDSTKLTLRLTRVRSSDMDQQEDMLSGMENSNVSENDIPFNVQYPGQTSKTPITPQDVNRPLNAAQCLSQQEQTAFLPANQVPVLQQNTSVATKQPQTSVVLNQQQVSQQGPIYDEVELDALAEIERIERESAIERERFSKEVQDKGSIDQSVLKELPPELLAEIESTMPLCERVKMNKRKRSTVNEKPKYAEISSDEDNDSDETFESSRKRHKKDDDKAWEYEERDRRSSGDHRRSGHSHEGRRSSGGGRYRNRSPSDSDMEDYSPPPSLSEVARKMKKKEKQKKRKAYEPKLTPEEMMDSSTFKRFTASIENILDNLEDMDFTAFGDDDEIPQELLLGKHQLNELGSESAKIKAMGIMDKLSTDKTVKVLNILEKNIQDGSKLSTLLNHNNDTEEEERLWRDLIMERVTKSADACLTTINIMTSPNMPKAVYIEDVIERVIQYTKFHLQNTLYPQYDPVYRLDPHGGGLLSSKAKRAKCSTHKQRVIVMLYNKVCDIVSSLSELLEIQLLTDTTILQVSSMGITPFFVENVSELQLCAIKLVTAVFSRYEKHRQLILEEIFTSLARLPTSKRSLRNFRLNSSDMDGEPMYIQMVTALVLQLIQCVVHLPSSEKDSNSEEDSNKKVDQDVIITNSYETAMRTAQNFLSIFLKKCGSKQGEEDYRPLFENFVQDLLSTVNKPEWPAAELLLSLLGRLLVHQFSNKSTEMALRVASLDYLGTVAARLRKDAVTSKMDQGSIERILKQVSGGEDEIQQLQKALLDYLDENTETDPSLVFSRKFYIAQWFRDTTLETEKAMKSQKDEESSEGSHHAKEVETTGQIMHRAENRKKFLRSIIKTTPSQFSTLKMNSDTVDYDDACLIVRYLASMRPFAQSFDIYLTQILRVLGENAIAVRTKAMKCLSEVVAVDPSILARLDMQRGVHGRLMDNSTSVREAAVELLGRFVLCRPQLAEQYYDMLIERILDTGISVRKRVIKILRDICIEQPTFPKITEMCVKMIRRVNDEEGIKKLVNETFQKLWFTPTPHNDKEAMTRKILNITDVVAACRDTGYDWFEQLLQNLLKSEEDSSYKPVKKACTQLVDNLVEHILKYEESLADSDNKGVNSGRLVACITTLFLFSKIRPQLMVKHAMTMQPYLTTKCSTQNDFMVICNVAKILELVVPLMEHPSETFLATIEEDLMKLIIKYGMTVVQHCVSCLGAVVNKVTQNFKFVWACFNRYYGAISKLKSQHQEDPNNTSLLTNKPALLRSLFTVGALCRHFDFDLEDFKGNSKVNIKDKVLELLMYFTKHSDEEVQTKAIIGLGFAFIQHPSLMFEQEVKNLYNNILSDKNSSVNLKIQVLKNLQTYLQEEDTRMQQADRDWKKVAKQEDLKEMGDVSSGMSSSIMQLYLKQVLEAFFHTQSSVRHFALNVIALTLNQGLIHPVQCVPYLIAMGTDPEPAMRNKADQQLVEIDKKYAGFIHMKAVAGMKMSYQVQQAINTCLKDPVRGFRQDESSSALCSHLYSMIRGNRQHRRAFLISLLNLFDDTAKTEVNMLLYIADNLACFPYQTQEEPLFIMHHIDITLSVSGSNLLQSFKESMVKDKRKERKSSPGKENESSESEEEVSRPRKSRKRVDSDSDSDSEDDINSVMKCLPENSAPLIEFANVSQGILLLLMLKQHLKNLCGFSDSKIQKYSPSESAKVYDKAINRKTGVHFHPKQTLDFLRSDMANSKITEDVKRSIVKQYLDFKLLMEHLDPDEEEEEGEVSASTNARNKAITSLLGGGSPKNNAAAETEDDESDGEDRGGGTSGSLRRSKRNSDSTELAAQMNESVDVMDVIAICCPKYKDRPQIARVVQKTSSGFSVQWMAGSYSGSWTEAKRRDGRKLVPWVDTIKESDIIYKKIALTSANKLTNKVVQTLRSLYAAKDGTSS

Target ORF information:

RefSeq Version	XM_015459095.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Nipped-B homolog (Drosophila) (NIPBL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015459095.1

ORF Insert Sequence:

ATGAATGGGG ATATGCCCCA TGTCCCCATC ACTACTCTTG CGGGGATTGC TAGTCTTACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCC CCTTTACCTG CTACAACTAC AAAGAGCCTT 
CTCTTTAATG CACGAATAGC AGAAGAAGTG AACTGCCTTT TGGCCTGTAG GGATGATAAT 
TTGGTTTCAC AGCTTGTCCA CAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA 
GATAACCTTG GCAGTGATGA TCCAGAAGGC GACATACCAG TCTTGTTGCA GGCCGTCCTG 
GCGAGGAGTC CTAACGTTTT CAGGGAGAAA AGTATGCAGA ACAGATATGT ACAAAGTGGA 
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC 
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA 
AGCTCTGGGA ACAGATTTAT GCCACAACAA AATAGCCCAG TGCCTAGTCC ATATGCCCCA 
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTATACAAC ACATCCACAG 
ATGCAGCAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CCTACATGAT 
AATAAAGTTT CTGGTTCGTT GTCTGGCAAT TCTGCTAATC ATCATGCTGA TAATCCTAGA 
CATGGCTCAA GTGACGACTA CCTACACATG GTGCACAGGA TAAGTAGTGA CGATGGAGAT 
TCTTCAACAA TGAGGAATGC TGCGTCTTTT CCCTTGAGGT CTCCACAGCC AGTATGTTCC 
CCTGCTGGAA GTGATGGGAC TCCAAAAGGA TCAAGACCAT CTTTAATCCT GCAGTCTCAG 
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGACA TCTTGTTAGA TTCTCCAGAA 
AGAAAGCAAA AGAAGCAAAA GAAAATGAAA TTAGGCAAAG ATGAAAAAGA CCAGAATGAG 
AAGGCTGCAA TGTATGATAT CATTAGCTCT CCATCCAAGG ACTCCACTAA ACTTACGTTA 
AGACTTACTC GTGTAAGATC TTCAGACATG GACCAGCAAG AGGATATGCT TTCTGGTATG 
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT 
TCAAAAACAC CCATTACTCC ACAGGATGTA AACCGCCCAC TAAATGCTGC TCAGTGTTTG 
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC 
ACTTCAGTTG CTACAAAACA GCCCCAGACT TCTGTGGTAC TGAATCAGCA ACAGGTATCA 
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA 
GAGAGAGAAT CAGCTATTGA AAGGGAGCGT TTTTCAAAAG AAGTTCAAGA TAAAGGTAGT 
ATAGATCAAT CAGTGTTAAA AGAATTACCC CCTGAACTCC TGGCAGAAAT TGAATCCACC 
ATGCCACTTT GTGAACGTGT GAAAATGAAT AAACGCAAGC GTAGCACAGT TAATGAAAAA 
CCAAAATATG CTGAAATCAG TTCAGATGAA GATAATGATA GTGATGAAAC TTTTGAATCC 
TCTAGGAAAC GACATAAAAA AGATGATGAT AAAGCTTGGG AATATGAAGA GCGTGACAGA 
AGAAGCTCTG GGGATCATAG GAGAAGTGGC CACTCTCATG AAGGAAGAAG AAGTTCAGGT 
GGTGGTCGTT ATCGAAACCG AAGCCCATCA GATTCTGACA TGGAAGATTA TTCTCCTCCT 
CCCAGCCTTA GTGAGGTCGC TAGAAAAATG AAGAAAAAAG AAAAACAGAA GAAGCGGAAA 
GCTTATGAAC CAAAACTAAC ACCCGAAGAA ATGATGGATT CTTCAACTTT TAAGAGATTC 
ACAGCCTCAA TAGAGAATAT TTTGGATAAC TTAGAAGATA TGGATTTTAC TGCATTTGGT 
GATGATGATG AAATTCCTCA GGAACTGCTC TTAGGAAAAC ATCAGCTTAA TGAACTTGGC 
AGTGAATCTG CTAAAATTAA AGCAATGGGT ATAATGGATA AGCTTTCCAC TGACAAAACT 
GTGAAAGTCC TAAATATCTT GGAAAAGAAT ATTCAAGATG GGTCAAAGCT CTCCACTTTG 
TTAAATCATA ATAATGATAC TGAAGAAGAA GAAAGGTTAT GGAGAGACCT TATTATGGAG 
AGAGTGACAA AATCAGCAGA TGCCTGTCTT ACAACTATAA ACATCATGAC ATCCCCTAAC 
ATGCCAAAAG CTGTATATAT TGAGGATGTA ATCGAAAGAG TTATACAGTA CACTAAATTT 
CATTTGCAGA ATACACTTTA TCCTCAGTAT GATCCTGTTT ACAGATTAGA TCCTCATGGA 
GGAGGCTTAC TAAGTTCAAA GGCAAAACGG GCTAAATGTT CTACCCATAA GCAGAGAGTA 
ATAGTAATGC TTTATAATAA AGTTTGTGAC ATTGTTAGCA GCTTATCAGA ACTACTAGAA 
ATACAACTTC TAACAGACAC AACAATCCTT CAGGTATCTT CAATGGGAAT AACACCATTT 
TTTGTGGAAA ATGTCAGTGA ACTACAATTG TGTGCCATTA AATTAGTTAC TGCAGTATTC 
TCAAGATATG AAAAACATAG GCAGTTAATT TTGGAAGAAA TTTTTACTTC ACTGGCAAGA 
TTACCTACCA GCAAGAGGAG CTTAAGGAAC TTCAGGTTAA ATAGTAGTGA TATGGATGGA 
GAGCCTATGT ATATTCAGAT GGTTACAGCA CTGGTTTTAC AACTAATTCA GTGTGTAGTA 
CACTTACCAT CATCAGAGAA GGACTCTAAT TCAGAAGAGG ACTCAAATAA AAAAGTTGAT 
CAAGATGTTA TCATTACCAA TTCTTATGAA ACAGCCATGC GAACAGCCCA AAACTTCCTT 
TCCATCTTCC TTAAAAAATG TGGTAGTAAG CAAGGTGAAG AAGATTACAG ACCACTATTT 
GAAAATTTTG TTCAAGACCT TCTTTCAACA GTCAATAAGC CTGAATGGCC AGCTGCTGAA 
CTACTCCTTA GTTTGTTAGG GAGACTGTTG GTTCATCAGT TCAGTAATAA GTCAACAGAG 
ATGGCTTTAA GAGTGGCATC CCTTGATTAC CTTGGAACTG TCGCTGCACG ACTGAGGAAA 
GATGCTGTTA CAAGCAAAAT GGATCAAGGG TCTATAGAAC GAATTTTAAA ACAGGTTTCA 
GGAGGGGAAG ATGAAATACA GCAATTACAA AAAGCATTGC TTGATTACTT GGATGAAAAC 
ACGGAGACTG ATCCTTCACT GGTGTTTTCT CGTAAATTCT ATATTGCCCA GTGGTTCCGT 
GATACAACAC TGGAAACAGA AAAAGCAATG AAATCACAAA AAGATGAAGA ATCATCTGAA 
GGATCACATC ATGCAAAGGA AGTTGAGACA ACTGGTCAAA TTATGCATCG AGCTGAAAAC 
CGGAAGAAGT TTCTTAGAAG CATTATCAAA ACCACACCTT CTCAGTTCAG CACATTAAAA 
ATGAACTCTG ATACTGTGGA CTATGATGAT GCTTGTTTGA TTGTTCGATA CTTGGCCTCT 
ATGAGGCCGT TTGCCCAGAG CTTTGATATT TATTTGACAC AGATCCTTCG AGTCCTTGGT 
GAAAATGCAA TTGCCGTTCG AACAAAAGCC ATGAAGTGTT TATCTGAGGT TGTTGCTGTA 
GACCCCAGTA TTCTAGCAAG GCTGGATATG CAGCGAGGAG TTCATGGACG ATTAATGGAT 
AACTCCACTA GTGTCAGAGA AGCAGCAGTG GAATTACTAG GTCGATTTGT CCTTTGTCGA 
CCTCAGCTTG CTGAGCAGTA TTATGATATG CTGATTGAAA GAATATTGGA TACTGGTATC 
AGTGTCAGAA AAAGGGTGAT AAAGATTCTA AGAGACATCT GTATTGAACA ACCGACATTT 
CCAAAAATTA CAGAAATGTG TGTAAAAATG ATTCGCAGGG TAAATGATGA AGAGGGCATT 
AAGAAATTAG TAAATGAAAC ATTCCAAAAA CTCTGGTTTA CTCCAACTCC ACACAATGAC 
AAAGAAGCAA TGACAAGGAA GATTTTAAAT ATCACTGATG TGGTTGCAGC ATGCAGAGAT 
ACTGGATATG ACTGGTTTGA GCAGCTTCTT CAAAATTTGT TGAAGTCTGA AGAGGATTCC 
TCATATAAAC CTGTGAAGAA AGCTTGTACT CAACTTGTTG ATAACCTAGT TGAGCACATT 
CTTAAATATG AGGAATCTCT AGCTGACTCT GACAATAAAG GTGTGAATTC TGGAAGATTG 
GTAGCTTGCA TAACCACTTT GTTCTTATTC AGCAAAATAA GACCCCAGCT CATGGTTAAA 
CATGCCATGA CTATGCAGCC ATATCTTACC ACTAAATGTA GTACACAAAA TGATTTCATG 
GTTATCTGCA ATGTTGCAAA AATTCTAGAA CTAGTTGTAC CACTGATGGA ACATCCGAGT 
GAAACTTTCC TTGCCACTAT TGAAGAAGAT CTAATGAAGC TTATCATCAA ATATGGCATG 
ACTGTAGTGC AACATTGTGT AAGCTGTCTT GGGGCTGTTG TAAATAAAGT GACACAAAAT 
TTTAAATTTG TGTGGGCCTG TTTCAATAGA TACTATGGTG CCATTTCAAA ATTAAAAAGT 
CAACACCAAG AGGACCCAAA TAACACTTCA CTGCTAACAA ACAAACCAGC ACTTTTGAGA 
TCCCTATTCA CTGTTGGGGC ACTGTGTCGG CATTTTGATT TTGATCTGGA AGATTTTAAA 
GGCAACAGTA AGGTTAACAT AAAGGATAAA GTACTTGAAC TATTGATGTA TTTTACAAAG 
CATTCAGATG AAGAAGTGCA AACAAAAGCC ATCATTGGTC TAGGATTTGC CTTTATTCAG 
CACCCAAGTC TAATGTTTGA GCAAGAGGTG AAAAATCTAT ACAATAATAT TTTATCTGAT 
AAAAACTCCT CAGTAAATTT AAAAATACAA GTGTTAAAAA ACCTCCAGAC CTACCTACAA 
GAAGAAGATA CACGTATGCA GCAGGCAGAT AGAGACTGGA AGAAGGTTGC AAAACAGGAA 
GATTTGAAAG AAATGGGTGA TGTTTCCTCA GGGATGAGTA GTTCCATCAT GCAGCTTTAC 
CTCAAACAGG TGCTTGAGGC GTTTTTTCAC ACCCAATCAA GTGTACGCCA CTTCGCCCTC 
AATGTCATTG CATTGACTCT AAACCAAGGT CTTATTCATC CAGTTCAGTG TGTGCCGTAT 
TTAATTGCTA TGGGCACAGA CCCAGAACCA GCTATGCGGA ACAAGGCTGA TCAGCAGCTT 
GTGGAAATAG ACAAAAAATA TGCTGGATTC ATTCACATGA AAGCAGTGGC TGGCATGAAG 
ATGTCTTACC AGGTACAACA GGCAATCAAC ACGTGCCTAA AAGATCCTGT AAGGGGTTTC 
CGGCAGGACG AGTCCTCCAG TGCTTTGTGT TCACACCTTT ACTCCATGAT CCGTGGAAAC 
CGCCAGCACA GACGCGCCTT TCTGATTTCT TTACTCAACC TCTTTGATGA CACAGCAAAA 
ACAGAAGTGA ATATGCTCTT GTATATAGCA GACAATCTAG CCTGTTTTCC ATACCAGACA 
CAGGAAGAGC CGTTGTTTAT AATGCATCAT ATAGACATTA CACTCTCTGT TTCTGGTAGT 
AACCTACTGC AGTCATTCAA GGAGTCCATG GTAAAAGACA AAAGGAAAGA GAGAAAATCA 
TCTCCTGGTA AGGAAAATGA GTCAAGCGAG AGTGAAGAAG AAGTCTCTAG GCCTCGAAAG 
TCACGGAAAC GTGTAGATTC AGATTCAGAT TCGGATTCAG AAGATGATAT AAATTCGGTG 
ATGAAATGTT TGCCAGAAAA TTCAGCTCCT TTAATTGAGT TTGCAAATGT CTCCCAGGGT 
ATTTTATTGC TTCTGATGTT AAAGCAACAT TTGAAGAATC TCTGTGGATT TTCTGATAGT 
AAAATTCAGA AATACTCTCC ATCCGAATCT GCAAAAGTGT ATGATAAAGC GATAAACCGG 
AAAACAGGAG TTCATTTTCA TCCCAAGCAA ACACTGGACT TCCTGCGGAG TGATATGGCT 
AATTCCAAAA TCACTGAAGA TGTGAAGAGG AGTATAGTAA AACAGTACCT AGATTTCAAA 
CTTCTCATGG AACATCTGGA CCCTGATGAA GAGGAAGAAG AAGGGGAGGT GTCAGCCAGC 
ACAAATGCTC GGAACAAAGC AATTACCTCG CTGCTTGGAG GAGGCAGCCC TAAAAATAAT 
GCAGCAGCAG AGACAGAAGA TGATGAAAGT GATGGGGAGG ATAGAGGAGG AGGCACTTCA 
GGGTCATTGA GAAGGTCAAA ACGAAATTCA GACTCTACGG AGTTGGCAGC ACAGATGAAT 
GAAAGTGTTG ACGTCATGGA TGTCATCGCT ATTTGCTGTC CAAAGTACAA AGACCGACCA 
CAGATTGCAA GAGTAGTACA GAAAACCAGC AGTGGCTTCA GTGTTCAGTG GATGGCAGGC 
TCCTACAGTG GCTCCTGGAC TGAGGCTAAG CGCCGTGATG GCCGCAAACT GGTGCCTTGG 
GTAGACACTA TTAAAGAGTC AGACATTATT TACAAAAAAA TTGCTCTAAC GAGTGCTAAT 
AAGCTGACTA ATAAAGTTGT TCAGACTTTA CGATCCCTGT ATGCCGCCAA GGATGGGACT 
TCCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIPBL ( NM_001206584.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001193513.1 Bos taurus NIPBL cohesin loading factor (NIPBL), mRNA.

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CloneID	OBa239220
Clone ID Related Accession (Same CDS sequence)	NM_001206584.2
Accession Version	NM_001206584.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8415bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1548691200000
Organism	Bos taurus(cattle)
Product	nipped-B-like protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000020.1. On Dec 20, 2018 this sequence version replaced NM_001206584.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GGUY01036956.1, GGVC01060376.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGAATGGGG ATATGCCCCA TGTCCCCATC ACTACTCTTG CGGGGATTGC TAGTCTTACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCC CCTTTACCTG CTACAACTAC AAAGAGCCTT 
CTCTTTAATG CACGAATAGC AGAAGAAGTG AACTGCCTTT TGGCCTGTAG GGATGATAAT 
TTGGTTTCAC AGCTTGTCCA CAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA 
GATAACCTTG GCAGTGATGA TCCAGAAGGC GACATACCAG TCTTGTTGCA GGCCGTCCTG 
GCGAGGAGTC CTAACGTTTT CAGGGAGAAA AGTATGCAGA ACAGATATGT ACAAAGTGGA 
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC 
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA 
AGCTCTGGGA ACAGATTTAT GCCACAACAA AATAGCCCAG TGCCTAGTCC ATATGCCCCA 
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTATACAAC ACATCCACAG 
ATGCAGCAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CCTACATGAT 
AATAAAGTTT CTGGTTCGTT GTCTGGCAAT TCTGCTAATC ATCATGCTGA TAATCCTAGA 
CATGGCTCAA GTGACGACTA CCTACACATG GTGCACAGGA TAAGTAGTGA CGATGGAGAT 
TCTTCAACAA TGAGGAATGC TGCGTCTTTT CCCTTGAGGT CTCCACAGCC AGTATGTTCC 
CCTGCTGGAA GTGATGGGAC TCCAAAAGGA TCAAGACCAT CTTTAATCCT GCAGTCTCAG 
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGACA TCTTGTTAGA TTCTCCAGAA 
AGAAAGCAAA AGAAGCAAAA GAAAATGAAA TTAGGCAAAG ATGAAAAAGA CCAGAATGAG 
AAGGCTGCAA TGTATGATAT CATTAGCTCT CCATCCAAGG ACTCCACTAA ACTTACGTTA 
AGACTTACTC GTGTAAGATC TTCAGACATG GACCAGCAAG AGGATATGCT TTCTGGTATG 
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT 
TCAAAAACAC CCATTACTCC ACAGGATGTA AACCGCCCAC TAAATGCTGC TCAGTGTTTG 
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC 
ACTTCAGTTG CTACAAAACA GCCCCAGACT TCTGTGGTAC TGAATCAGCA ACAGGTATCA 
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA 
GAGAGAGAAT CAGCTATTGA AAGGGAGCGT TTTTCAAAAG AAGTTCAAGA TAAAGATAAG 
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT 
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG 
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCCTCTAT AACTCAGGAT 
TCAGACTCCA TTAAAAAGCC TGAAGAAATC AAACAATGTA ATGACACACC TATTTCTGTT 
CTTCAGGAAG ATATTATTGG AAATCTTAAA TCTACACCAG AAAACCATCC TGAGACTCCT 
AAAAAAAAGT CTGATCCTGA GCTTTTAAAG AGTGAAGTGA AACAGAATGA AAGTAGATTG 
GCAGAGTCTA AACCAAATGA AAACAGGTTG GTAGAGACAA AATCTAGTGA AACTAAGTTA 
GAAACTAAAA TTGAGGTACC AACAGAAGAA CCTAAACAGA ATGAGAACAG AATGATTGAA 
TCCAGACAAA ATGAGAGCAG CACAGCCGAG CCTAAACAAA ATGAAAATAG ACTGCCGGAC 
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGACCC 
GAAACCCCAA AGCAAAAGGG TGAAAGCCGA CCTGAAACTC CAAAACAAAA GAGTGATGGG 
CGTCCTGAAA CCCCCAAACA AAAGGGTGAT GGACGGCCTG AAACTCCAAA GCAGAAAGGT 
GAGGGCCGAC CTGAAACTCC AAAGCAAAAA AATGAAGGTC GGCCTGAAAC ACCGAAACAT 
AGACATGAAA ATAGGAGGGA TTCTGGAAAG CCATCAACAG AGAAAAAACC TGAAGTGTCT 
AAACATAAAC AGGACATTAA ATCTGATTCA CCTCGGTTAA AATCAGAAAG AGCTGAAGCC 
TTAAAGCAGA GACCTGATGG GCGATCCATC TCTGAGTCAC TAAGACGTGA TCATGACAGT 
AAACAAAAGT CAGATGACAG GAGTGAATCT GAACGACATC GAGGGGATCA ATCTAGGGTT 
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGACC ATGGCATTAA ATCTGATGGT 
TCAAAACCTG ATAAACTAGA AAGAAAACAC AGACATGAAT CAGGGGACTC AAGGGAAAGA 
CCATCTTCTG GGGAACAAAA GTCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT 
AATAAATCAA GACCTGATAA GCCTGGTTTT AAATCTCCAA ATAGTAAGGA TGACAGAAGG 
ACAGAGGGTA ACAAGAGTAA AGTAGACAGT AATAAAACGC ACCCTGATAA TAAGGCAGAA 
TTTCCAAGTT ATTTGCTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCAAAA 
ATCAAGAGGG ATAAAGATGG CAATATTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA 
GAGCAGAAAG ACAAAGTAGA AAAAATGGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG 
CCTGTAGTTG TCCTGCAAAA ACTGTCTTTG GATGATGTTC AAAAACTTAT TAAAGATAGA 
GAGGATAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA 
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAA 
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAATAAAC GCAAGCGTAG CACAGTTAAT 
GAAAAACCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAACTTTT 
GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT 
GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGAAGT 
TCAGGTGGTG GTCGTTATCG AAACCGAAGC CCATCAGATT CTGACATGGA AGATTATTCT 
CCTCCTCCCA GCCTTAGTGA GGTCGCTAGA AAAATGAAGA AAAAAGAAAA ACAGAAGAAG 
CGGAAAGCTT ATGAACCAAA ACTAACACCC GAAGAAATGA TGGATTCTTC AACTTTTAAG 
AGATTCACAG CCTCAATAGA GAATATTTTG GATAACTTAG AAGATATGGA TTTTACTGCA 
TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA 
CTTGGCAGTG AATCTGCTAA AATTAAAGCA ATGGGTATAA TGGATAAGCT TTCCACTGAC 
AAAACTGTGA AAGTCCTAAA TATCTTGGAA AAGAATATTC AAGATGGGTC AAAGCTCTCC 
ACTTTGTTAA ATCATAATAA TGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT 
ATGGAGAGAG TGACAAAATC AGCAGATGCC TGTCTTACAA CTATAAACAT CATGACATCC 
CCTAACATGC CAAAAGCTGT ATATATTGAG GATGTAATCG AAAGAGTTAT ACAGTACACT 
AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT 
CATGGAGGAG GCTTACTAAG TTCAAAGGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG 
AGAGTAATAG TAATGCTTTA TAATAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAACTA 
CTAGAAATAC AACTTCTAAC AGACACAACA ATCCTTCAGG TATCTTCAAT GGGAATAACA 
CCATTTTTTG TGGAAAATGT CAGTGAACTA CAATTGTGTG CCATTAAATT AGTTACTGCA 
GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTG 
GCAAGATTAC CTACCAGCAA GAGGAGCTTA AGGAACTTCA GGTTAAATAG TAGTGATATG 
GATGGAGAGC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT AATTCAGTGT 
GTAGTACACT TACCATCATC AGAGAAGGAC TCTAATTCAG AAGAGGACTC AAATAAAAAA 
GTTGATCAAG ATGTTATCAT TACCAATTCT TATGAAACAG CCATGCGAAC AGCCCAAAAC 
TTCCTTTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA 
CTATTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT 
GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAATAAGTCA 
ACAGAGATGG CTTTAAGAGT GGCATCCCTT GATTACCTTG GAACTGTCGC TGCACGACTG 
AGGAAAGATG CTGTTACAAG CAAAATGGAT CAAGGGTCTA TAGAACGAAT TTTAAAACAG 
GTTTCAGGAG GGGAAGATGA AATACAGCAA TTACAAAAAG CATTGCTTGA TTACTTGGAT 
GAAAACACGG AGACTGATCC TTCACTGGTG TTTTCTCGTA AATTCTATAT TGCCCAGTGG 
TTCCGTGATA CAACACTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA 
TCTGAAGGAT CACATCATGC AAAGGAAGTT GAGACAACTG GTCAAATTAT GCATCGAGCT 
GAAAACCGGA AGAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTCAGCACA 
TTAAAAATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GTTTGATTGT TCGATACTTG 
GCCTCTATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTTCGAGTC 
CTTGGTGAAA ATGCAATTGC CGTTCGAACA AAAGCCATGA AGTGTTTATC TGAGGTTGTT 
GCTGTAGACC CCAGTATTCT AGCAAGGCTG GATATGCAGC GAGGAGTTCA TGGACGATTA 
ATGGATAACT CCACTAGTGT CAGAGAAGCA GCAGTGGAAT TACTAGGTCG ATTTGTCCTT 
TGTCGACCTC AGCTTGCTGA GCAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT 
GGTATCAGTG TCAGAAAAAG GGTGATAAAG ATTCTAAGAG ACATCTGTAT TGAACAACCG 
ACATTTCCAA AAATTACAGA AATGTGTGTA AAAATGATTC GCAGGGTAAA TGATGAAGAG 
GGCATTAAGA AATTAGTAAA TGAAACATTC CAAAAACTCT GGTTTACTCC AACTCCACAC 
AATGACAAAG AAGCAATGAC AAGGAAGATT TTAAATATCA CTGATGTGGT TGCAGCATGC 
AGAGATACTG GATATGACTG GTTTGAGCAG CTTCTTCAAA ATTTGTTGAA GTCTGAAGAG 
GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG 
CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA 
AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG 
GTTAAACATG CCATGACTAT GCAGCCATAT CTTACCACTA AATGTAGTAC ACAAAATGAT 
TTCATGGTTA TCTGCAATGT TGCAAAAATT CTAGAACTAG TTGTACCACT GATGGAACAT 
CCGAGTGAAA CTTTCCTTGC CACTATTGAA GAAGATCTAA TGAAGCTTAT CATCAAATAT 
GGCATGACTG TAGTGCAACA TTGTGTAAGC TGTCTTGGGG CTGTTGTAAA TAAAGTGACA 
CAAAATTTTA AATTTGTGTG GGCCTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA 
AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTGC TAACAAACAA ACCAGCACTT 
TTGAGATCCC TATTCACTGT TGGGGCACTG TGTCGGCATT TTGATTTTGA TCTGGAAGAT 
TTTAAAGGCA ACAGTAAGGT TAACATAAAG GATAAAGTAC TTGAACTATT GATGTATTTT 
ACAAAGCATT CAGATGAAGA AGTGCAAACA AAAGCCATCA TTGGTCTAGG ATTTGCCTTT 
ATTCAGCACC CAAGTCTAAT GTTTGAGCAA GAGGTGAAAA ATCTATACAA TAATATTTTA 
TCTGATAAAA ACTCCTCAGT AAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC 
CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA GGTTGCAAAA 
CAGGAAGATT TGAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG 
CTTTACCTCA AACAGGTGCT TGAGGCGTTT TTTCACACCC AATCAAGTGT ACGCCACTTC 
GCCCTCAATG TCATTGCATT GACTCTAAAC CAAGGTCTTA TTCATCCAGT TCAGTGTGTG 
CCGTATTTAA TTGCTATGGG CACAGACCCA GAACCAGCTA TGCGGAACAA GGCTGATCAG 
CAGCTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ACATGAAAGC AGTGGCTGGC 
ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACGT GCCTAAAAGA TCCTGTAAGG 
GGTTTCCGGC AGGACGAGTC CTCCAGTGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT 
GGAAACCGCC AGCACAGACG CGCCTTTCTG ATTTCTTTAC TCAACCTCTT TGATGACACA 
GCAAAAACAG AAGTGAATAT GCTCTTGTAT ATAGCAGACA ATCTAGCCTG TTTTCCATAC 
CAGACACAGG AAGAGCCGTT GTTTATAATG CATCATATAG ACATTACACT CTCTGTTTCT 
GGTAGTAACC TACTGCAGTC ATTCAAGGAG TCCATGGTAA AAGACAAAAG GAAAGAGAGA 
AAATCATCTC CTGGTAAGGA AAATGAGTCA AGCGAGAGTG AAGAAGAAGT CTCTAGGCCT 
CGAAAGTCAC GGAAACGTGT AGATTCAGAT TCAGATTCGG ATTCAGAAGA TGATATAAAT 
TCGGTGATGA AATGTTTGCC AGAAAATTCA GCTCCTTTAA TTGAGTTTGC AAATGTCTCC 
CAGGGTATTT TATTGCTTCT GATGTTAAAG CAACATTTGA AGAATCTCTG TGGATTTTCT 
GATAGTAAAA TTCAGAAATA CTCTCCATCC GAATCTGCAA AAGTGTATGA TAAAGCGATA 
AACCGGAAAA CAGGAGTTCA TTTTCATCCC AAGCAAACAC TGGACTTCCT GCGGAGTGAT 
ATGGCTAATT CCAAAATCAC TGAAGATGTG AAGAGGAGTA TAGTAAAACA GTACCTAGAT 
TTCAAACTTC TCATGGAACA TCTGGACCCT GATGAAGAGG AAGAAGAAGG GGAGGTGTCA 
GCCAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCGCTGC TTGGAGGAGG CAGCCCTAAA 
AATAATGCAG CAGCAGAGAC AGAAGATGAT GAAAGTGATG GGGAGGATAG AGGAGGAGGC 
ACTTCAGGGT CATTGAGAAG GTCAAAACGA AATTCAGACT CTACGGAGTT GGCAGCACAG 
ATGAATGAAA GTGTTGACGT CATGGATGTC ATCGCTATTT GCTGTCCAAA GTACAAAGAC 
CGACCACAGA TTGCAAGAGT AGTACAGAAA ACCAGCAGTG GCTTCAGTGT TCAGTGGATG 
GCAGGCTCCT ACAGTGGCTC CTGGACTGAG GCTAAGCGCC GTGATGGCCG CAAACTGGTG 
CCTTGGGTAG ACACTATTAA AGAGTCAGAC ATTATTTACA AAAAAATTGC TCTAACGAGT 
GCTAATAAGC TGACTAATAA AGTTGTTCAG ACTTTACGAT CCCTGTATGC CGCCAAGGAT 
GGGACTTCCA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001193513.1
CDS	502..8916
Translation	MNGDMPHVPITTLAGIASLTDLLNQLPLPSPLPATTTKSLLFNARIAEEVNCLLACRDDNLVSQLVHSLNQVSTDHIELKDNLGSDDPEGDIPVLLQAVLARSPNVFREKSMQNRYVQSGMMMSQYKLSQNSMHSSPASSNYQQTTISHSPSSRFVPPQTSSGNRFMPQQNSPVPSPYAPQSPAGYMPYSHPSSYTTHPQMQQASVSSPIVAGGLRNLHDNKVSGSLSGNSANHHADNPRHGSSDDYLHMVHRISSDDGDSSTMRNAASFPLRSPQPVCSPAGSDGTPKGSRPSLILQSQSLPCSSPRDVPPDILLDSPERKQKKQKKMKLGKDEKDQNEKAAMYDIISSPSKDSTKLTLRLTRVRSSDMDQQEDMLSGMENSNVSENDIPFNVQYPGQTSKTPITPQDVNRPLNAAQCLSQQEQTAFLPANQVPVLQQNTSVATKQPQTSVVLNQQQVSQQGPIYDEVELDALAEIERIERESAIERERFSKEVQDKDKPLKKRKQDSYPQEAGGATGGNRPASQETGSTGNGSRPALMVSIDLHQAGRVDSQASITQDSDSIKKPEEIKQCNDTPISVLQEDIIGNLKSTPENHPETPKKKSDPELLKSEVKQNESRLAESKPNENRLVETKSSETKLETKIEVPTEEPKQNENRMIESRQNESSTAEPKQNENRLPDTKPNDNKQNNGRSETTKSRPETPKQKGESRPETPKQKSDGRPETPKQKGDGRPETPKQKGEGRPETPKQKNEGRPETPKHRHENRRDSGKPSTEKKPEVSKHKQDIKSDSPRLKSERAEALKQRPDGRSISESLRRDHDSKQKSDDRSESERHRGDQSRVRRPETLRSSSRNDHGIKSDGSKPDKLERKHRHESGDSRERPSSGEQKSRPDSPRVKQGDSNKSRPDKPGFKSPNSKDDRRTEGNKSKVDSNKTHPDNKAEFPSYLLGGRSGALKNFVIPKIKRDKDGNITQETKKMEMKGEQKDKVEKMGLVEDLNKGAKPVVVLQKLSLDDVQKLIKDREDKSRSSLKPIKNKPSKSNKGSIDQSVLKELPPELLAEIESTMPLCERVKMNKRKRSTVNEKPKYAEISSDEDNDSDETFESSRKRHKKDDDKAWEYEERDRRSSGDHRRSGHSHEGRRSSGGGRYRNRSPSDSDMEDYSPPPSLSEVARKMKKKEKQKKRKAYEPKLTPEEMMDSSTFKRFTASIENILDNLEDMDFTAFGDDDEIPQELLLGKHQLNELGSESAKIKAMGIMDKLSTDKTVKVLNILEKNIQDGSKLSTLLNHNNDTEEEERLWRDLIMERVTKSADACLTTINIMTSPNMPKAVYIEDVIERVIQYTKFHLQNTLYPQYDPVYRLDPHGGGLLSSKAKRAKCSTHKQRVIVMLYNKVCDIVSSLSELLEIQLLTDTTILQVSSMGITPFFVENVSELQLCAIKLVTAVFSRYEKHRQLILEEIFTSLARLPTSKRSLRNFRLNSSDMDGEPMYIQMVTALVLQLIQCVVHLPSSEKDSNSEEDSNKKVDQDVIITNSYETAMRTAQNFLSIFLKKCGSKQGEEDYRPLFENFVQDLLSTVNKPEWPAAELLLSLLGRLLVHQFSNKSTEMALRVASLDYLGTVAARLRKDAVTSKMDQGSIERILKQVSGGEDEIQQLQKALLDYLDENTETDPSLVFSRKFYIAQWFRDTTLETEKAMKSQKDEESSEGSHHAKEVETTGQIMHRAENRKKFLRSIIKTTPSQFSTLKMNSDTVDYDDACLIVRYLASMRPFAQSFDIYLTQILRVLGENAIAVRTKAMKCLSEVVAVDPSILARLDMQRGVHGRLMDNSTSVREAAVELLGRFVLCRPQLAEQYYDMLIERILDTGISVRKRVIKILRDICIEQPTFPKITEMCVKMIRRVNDEEGIKKLVNETFQKLWFTPTPHNDKEAMTRKILNITDVVAACRDTGYDWFEQLLQNLLKSEEDSSYKPVKKACTQLVDNLVEHILKYEESLADSDNKGVNSGRLVACITTLFLFSKIRPQLMVKHAMTMQPYLTTKCSTQNDFMVICNVAKILELVVPLMEHPSETFLATIEEDLMKLIIKYGMTVVQHCVSCLGAVVNKVTQNFKFVWACFNRYYGAISKLKSQHQEDPNNTSLLTNKPALLRSLFTVGALCRHFDFDLEDFKGNSKVNIKDKVLELLMYFTKHSDEEVQTKAIIGLGFAFIQHPSLMFEQEVKNLYNNILSDKNSSVNLKIQVLKNLQTYLQEEDTRMQQADRDWKKVAKQEDLKEMGDVSSGMSSSIMQLYLKQVLEAFFHTQSSVRHFALNVIALTLNQGLIHPVQCVPYLIAMGTDPEPAMRNKADQQLVEIDKKYAGFIHMKAVAGMKMSYQVQQAINTCLKDPVRGFRQDESSSALCSHLYSMIRGNRQHRRAFLISLLNLFDDTAKTEVNMLLYIADNLACFPYQTQEEPLFIMHHIDITLSVSGSNLLQSFKESMVKDKRKERKSSPGKENESSESEEEVSRPRKSRKRVDSDSDSDSEDDINSVMKCLPENSAPLIEFANVSQGILLLLMLKQHLKNLCGFSDSKIQKYSPSESAKVYDKAINRKTGVHFHPKQTLDFLRSDMANSKITEDVKRSIVKQYLDFKLLMEHLDPDEEEEEGEVSASTNARNKAITSLLGGGSPKNNAAAETEDDESDGEDRGGGTSGSLRRSKRNSDSTELAAQMNESVDVMDVIAICCPKYKDRPQIARVVQKTSSGFSVQWMAGSYSGSWTEAKRRDGRKLVPWVDTIKESDIIYKKIALTSANKLTNKVVQTLRSLYAAKDGTSS

Target ORF information:

RefSeq Version	NM_001206584.2
Organism	Bos taurus(cattle)
Definition	Bos taurus NIPBL cohesin loading factor (NIPBL), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001206584.2

ORF Insert Sequence:

ATGAATGGGG ATATGCCCCA TGTCCCCATC ACTACTCTTG CGGGGATTGC TAGTCTTACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCC CCTTTACCTG CTACAACTAC AAAGAGCCTT 
CTCTTTAATG CACGAATAGC AGAAGAAGTG AACTGCCTTT TGGCCTGTAG GGATGATAAT 
TTGGTTTCAC AGCTTGTCCA CAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA 
GATAACCTTG GCAGTGATGA TCCAGAAGGC GACATACCAG TCTTGTTGCA GGCCGTCCTG 
GCGAGGAGTC CTAACGTTTT CAGGGAGAAA AGTATGCAGA ACAGATATGT ACAAAGTGGA 
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC 
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA 
AGCTCTGGGA ACAGATTTAT GCCACAACAA AATAGCCCAG TGCCTAGTCC ATATGCCCCA 
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTATACAAC ACATCCACAG 
ATGCAGCAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CCTACATGAT 
AATAAAGTTT CTGGTTCGTT GTCTGGCAAT TCTGCTAATC ATCATGCTGA TAATCCTAGA 
CATGGCTCAA GTGACGACTA CCTACACATG GTGCACAGGA TAAGTAGTGA CGATGGAGAT 
TCTTCAACAA TGAGGAATGC TGCGTCTTTT CCCTTGAGGT CTCCACAGCC AGTATGTTCC 
CCTGCTGGAA GTGATGGGAC TCCAAAAGGA TCAAGACCAT CTTTAATCCT GCAGTCTCAG 
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGACA TCTTGTTAGA TTCTCCAGAA 
AGAAAGCAAA AGAAGCAAAA GAAAATGAAA TTAGGCAAAG ATGAAAAAGA CCAGAATGAG 
AAGGCTGCAA TGTATGATAT CATTAGCTCT CCATCCAAGG ACTCCACTAA ACTTACGTTA 
AGACTTACTC GTGTAAGATC TTCAGACATG GACCAGCAAG AGGATATGCT TTCTGGTATG 
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT 
TCAAAAACAC CCATTACTCC ACAGGATGTA AACCGCCCAC TAAATGCTGC TCAGTGTTTG 
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC 
ACTTCAGTTG CTACAAAACA GCCCCAGACT TCTGTGGTAC TGAATCAGCA ACAGGTATCA 
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA 
GAGAGAGAAT CAGCTATTGA AAGGGAGCGT TTTTCAAAAG AAGTTCAAGA TAAAGATAAG 
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT 
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG 
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCCTCTAT AACTCAGGAT 
TCAGACTCCA TTAAAAAGCC TGAAGAAATC AAACAATGTA ATGACACACC TATTTCTGTT 
CTTCAGGAAG ATATTATTGG AAATCTTAAA TCTACACCAG AAAACCATCC TGAGACTCCT 
AAAAAAAAGT CTGATCCTGA GCTTTTAAAG AGTGAAGTGA AACAGAATGA AAGTAGATTG 
GCAGAGTCTA AACCAAATGA AAACAGGTTG GTAGAGACAA AATCTAGTGA AACTAAGTTA 
GAAACTAAAA TTGAGGTACC AACAGAAGAA CCTAAACAGA ATGAGAACAG AATGATTGAA 
TCCAGACAAA ATGAGAGCAG CACAGCCGAG CCTAAACAAA ATGAAAATAG ACTGCCGGAC 
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGACCC 
GAAACCCCAA AGCAAAAGGG TGAAAGCCGA CCTGAAACTC CAAAACAAAA GAGTGATGGG 
CGTCCTGAAA CCCCCAAACA AAAGGGTGAT GGACGGCCTG AAACTCCAAA GCAGAAAGGT 
GAGGGCCGAC CTGAAACTCC AAAGCAAAAA AATGAAGGTC GGCCTGAAAC ACCGAAACAT 
AGACATGAAA ATAGGAGGGA TTCTGGAAAG CCATCAACAG AGAAAAAACC TGAAGTGTCT 
AAACATAAAC AGGACATTAA ATCTGATTCA CCTCGGTTAA AATCAGAAAG AGCTGAAGCC 
TTAAAGCAGA GACCTGATGG GCGATCCATC TCTGAGTCAC TAAGACGTGA TCATGACAGT 
AAACAAAAGT CAGATGACAG GAGTGAATCT GAACGACATC GAGGGGATCA ATCTAGGGTT 
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGACC ATGGCATTAA ATCTGATGGT 
TCAAAACCTG ATAAACTAGA AAGAAAACAC AGACATGAAT CAGGGGACTC AAGGGAAAGA 
CCATCTTCTG GGGAACAAAA GTCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT 
AATAAATCAA GACCTGATAA GCCTGGTTTT AAATCTCCAA ATAGTAAGGA TGACAGAAGG 
ACAGAGGGTA ACAAGAGTAA AGTAGACAGT AATAAAACGC ACCCTGATAA TAAGGCAGAA 
TTTCCAAGTT ATTTGCTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCAAAA 
ATCAAGAGGG ATAAAGATGG CAATATTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA 
GAGCAGAAAG ACAAAGTAGA AAAAATGGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG 
CCTGTAGTTG TCCTGCAAAA ACTGTCTTTG GATGATGTTC AAAAACTTAT TAAAGATAGA 
GAGGATAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA 
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAA 
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAATAAAC GCAAGCGTAG CACAGTTAAT 
GAAAAACCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAACTTTT 
GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT 
GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGAAGT 
TCAGGTGGTG GTCGTTATCG AAACCGAAGC CCATCAGATT CTGACATGGA AGATTATTCT 
CCTCCTCCCA GCCTTAGTGA GGTCGCTAGA AAAATGAAGA AAAAAGAAAA ACAGAAGAAG 
CGGAAAGCTT ATGAACCAAA ACTAACACCC GAAGAAATGA TGGATTCTTC AACTTTTAAG 
AGATTCACAG CCTCAATAGA GAATATTTTG GATAACTTAG AAGATATGGA TTTTACTGCA 
TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA 
CTTGGCAGTG AATCTGCTAA AATTAAAGCA ATGGGTATAA TGGATAAGCT TTCCACTGAC 
AAAACTGTGA AAGTCCTAAA TATCTTGGAA AAGAATATTC AAGATGGGTC AAAGCTCTCC 
ACTTTGTTAA ATCATAATAA TGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT 
ATGGAGAGAG TGACAAAATC AGCAGATGCC TGTCTTACAA CTATAAACAT CATGACATCC 
CCTAACATGC CAAAAGCTGT ATATATTGAG GATGTAATCG AAAGAGTTAT ACAGTACACT 
AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT 
CATGGAGGAG GCTTACTAAG TTCAAAGGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG 
AGAGTAATAG TAATGCTTTA TAATAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAACTA 
CTAGAAATAC AACTTCTAAC AGACACAACA ATCCTTCAGG TATCTTCAAT GGGAATAACA 
CCATTTTTTG TGGAAAATGT CAGTGAACTA CAATTGTGTG CCATTAAATT AGTTACTGCA 
GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTG 
GCAAGATTAC CTACCAGCAA GAGGAGCTTA AGGAACTTCA GGTTAAATAG TAGTGATATG 
GATGGAGAGC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT AATTCAGTGT 
GTAGTACACT TACCATCATC AGAGAAGGAC TCTAATTCAG AAGAGGACTC AAATAAAAAA 
GTTGATCAAG ATGTTATCAT TACCAATTCT TATGAAACAG CCATGCGAAC AGCCCAAAAC 
TTCCTTTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA 
CTATTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT 
GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAATAAGTCA 
ACAGAGATGG CTTTAAGAGT GGCATCCCTT GATTACCTTG GAACTGTCGC TGCACGACTG 
AGGAAAGATG CTGTTACAAG CAAAATGGAT CAAGGGTCTA TAGAACGAAT TTTAAAACAG 
GTTTCAGGAG GGGAAGATGA AATACAGCAA TTACAAAAAG CATTGCTTGA TTACTTGGAT 
GAAAACACGG AGACTGATCC TTCACTGGTG TTTTCTCGTA AATTCTATAT TGCCCAGTGG 
TTCCGTGATA CAACACTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA 
TCTGAAGGAT CACATCATGC AAAGGAAGTT GAGACAACTG GTCAAATTAT GCATCGAGCT 
GAAAACCGGA AGAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTCAGCACA 
TTAAAAATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GTTTGATTGT TCGATACTTG 
GCCTCTATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTTCGAGTC 
CTTGGTGAAA ATGCAATTGC CGTTCGAACA AAAGCCATGA AGTGTTTATC TGAGGTTGTT 
GCTGTAGACC CCAGTATTCT AGCAAGGCTG GATATGCAGC GAGGAGTTCA TGGACGATTA 
ATGGATAACT CCACTAGTGT CAGAGAAGCA GCAGTGGAAT TACTAGGTCG ATTTGTCCTT 
TGTCGACCTC AGCTTGCTGA GCAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT 
GGTATCAGTG TCAGAAAAAG GGTGATAAAG ATTCTAAGAG ACATCTGTAT TGAACAACCG 
ACATTTCCAA AAATTACAGA AATGTGTGTA AAAATGATTC GCAGGGTAAA TGATGAAGAG 
GGCATTAAGA AATTAGTAAA TGAAACATTC CAAAAACTCT GGTTTACTCC AACTCCACAC 
AATGACAAAG AAGCAATGAC AAGGAAGATT TTAAATATCA CTGATGTGGT TGCAGCATGC 
AGAGATACTG GATATGACTG GTTTGAGCAG CTTCTTCAAA ATTTGTTGAA GTCTGAAGAG 
GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG 
CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA 
AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG 
GTTAAACATG CCATGACTAT GCAGCCATAT CTTACCACTA AATGTAGTAC ACAAAATGAT 
TTCATGGTTA TCTGCAATGT TGCAAAAATT CTAGAACTAG TTGTACCACT GATGGAACAT 
CCGAGTGAAA CTTTCCTTGC CACTATTGAA GAAGATCTAA TGAAGCTTAT CATCAAATAT 
GGCATGACTG TAGTGCAACA TTGTGTAAGC TGTCTTGGGG CTGTTGTAAA TAAAGTGACA 
CAAAATTTTA AATTTGTGTG GGCCTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA 
AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTGC TAACAAACAA ACCAGCACTT 
TTGAGATCCC TATTCACTGT TGGGGCACTG TGTCGGCATT TTGATTTTGA TCTGGAAGAT 
TTTAAAGGCA ACAGTAAGGT TAACATAAAG GATAAAGTAC TTGAACTATT GATGTATTTT 
ACAAAGCATT CAGATGAAGA AGTGCAAACA AAAGCCATCA TTGGTCTAGG ATTTGCCTTT 
ATTCAGCACC CAAGTCTAAT GTTTGAGCAA GAGGTGAAAA ATCTATACAA TAATATTTTA 
TCTGATAAAA ACTCCTCAGT AAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC 
CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA GGTTGCAAAA 
CAGGAAGATT TGAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG 
CTTTACCTCA AACAGGTGCT TGAGGCGTTT TTTCACACCC AATCAAGTGT ACGCCACTTC 
GCCCTCAATG TCATTGCATT GACTCTAAAC CAAGGTCTTA TTCATCCAGT TCAGTGTGTG 
CCGTATTTAA TTGCTATGGG CACAGACCCA GAACCAGCTA TGCGGAACAA GGCTGATCAG 
CAGCTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ACATGAAAGC AGTGGCTGGC 
ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACGT GCCTAAAAGA TCCTGTAAGG 
GGTTTCCGGC AGGACGAGTC CTCCAGTGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT 
GGAAACCGCC AGCACAGACG CGCCTTTCTG ATTTCTTTAC TCAACCTCTT TGATGACACA 
GCAAAAACAG AAGTGAATAT GCTCTTGTAT ATAGCAGACA ATCTAGCCTG TTTTCCATAC 
CAGACACAGG AAGAGCCGTT GTTTATAATG CATCATATAG ACATTACACT CTCTGTTTCT 
GGTAGTAACC TACTGCAGTC ATTCAAGGAG TCCATGGTAA AAGACAAAAG GAAAGAGAGA 
AAATCATCTC CTGGTAAGGA AAATGAGTCA AGCGAGAGTG AAGAAGAAGT CTCTAGGCCT 
CGAAAGTCAC GGAAACGTGT AGATTCAGAT TCAGATTCGG ATTCAGAAGA TGATATAAAT 
TCGGTGATGA AATGTTTGCC AGAAAATTCA GCTCCTTTAA TTGAGTTTGC AAATGTCTCC 
CAGGGTATTT TATTGCTTCT GATGTTAAAG CAACATTTGA AGAATCTCTG TGGATTTTCT 
GATAGTAAAA TTCAGAAATA CTCTCCATCC GAATCTGCAA AAGTGTATGA TAAAGCGATA 
AACCGGAAAA CAGGAGTTCA TTTTCATCCC AAGCAAACAC TGGACTTCCT GCGGAGTGAT 
ATGGCTAATT CCAAAATCAC TGAAGATGTG AAGAGGAGTA TAGTAAAACA GTACCTAGAT 
TTCAAACTTC TCATGGAACA TCTGGACCCT GATGAAGAGG AAGAAGAAGG GGAGGTGTCA 
GCCAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCGCTGC TTGGAGGAGG CAGCCCTAAA 
AATAATGCAG CAGCAGAGAC AGAAGATGAT GAAAGTGATG GGGAGGATAG AGGAGGAGGC 
ACTTCAGGGT CATTGAGAAG GTCAAAACGA AATTCAGACT CTACGGAGTT GGCAGCACAG 
ATGAATGAAA GTGTTGACGT CATGGATGTC ATCGCTATTT GCTGTCCAAA GTACAAAGAC 
CGACCACAGA TTGCAAGAGT AGTACAGAAA ACCAGCAGTG GCTTCAGTGT TCAGTGGATG 
GCAGGCTCCT ACAGTGGCTC CTGGACTGAG GCTAAGCGCC GTGATGGCCG CAAACTGGTG 
CCTTGGGTAG ACACTATTAA AGAGTCAGAC ATTATTTACA AAAAAATTGC TCTAACGAGT 
GCTAATAAGC TGACTAATAA AGTTGTTCAG ACTTTACGAT CCCTGTATGC CGCCAAGGAT 
GGGACTTCCA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIPBL ( NM_001206584.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001193513.1 Bos taurus NIPBL, cohesin loading factor (NIPBL), mRNA.

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CloneID	OBa40744
Clone ID Related Accession (Same CDS sequence)	NM_001206584.1
Accession Version	NM_001206584.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8415bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1508601600000
Organism	Bos taurus(cattle)
Product	nipped-B-like protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DAAA02050625.1, DAAA02050622.1, DAAA02050620.1 and DAAA02050619.1. On or before May 4, 2011 this sequence version replaced XM_002696375.1, XM_588088.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00120237, SAMN00120238 [ECO:0000350] ##Evidence-Data-END##

ATGAATGGGG ATATGCCCCA TGTCCCCATC ACTACTCTTG CGGGGATTGC TAGTCTTACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCC CCTTTACCTG CTACAACTAC AAAGAGCCTT 
CTCTTTAATG CACGAATAGC AGAAGAAGTG AACTGCCTTT TGGCCTGTAG GGATGATAAT 
TTGGTTTCAC AGCTTGTCCA CAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA 
GATAACCTTG GCAGTGATGA TCCAGAAGGC GACATACCAG TCTTGTTGCA GGCCGTCCTG 
GCGAGGAGTC CTAACGTTTT CAGGGAGAAA AGTATGCAGA ACAGATATGT ACAAAGTGGA 
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC 
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA 
AGCTCTGGGA ACAGATTTAT GCCACAACAA AATAGCCCAG TGCCTAGTCC ATATGCCCCA 
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTATACAAC ACATCCACAG 
ATGCAGCAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CCTACATGAT 
AATAAAGTTT CTGGTTCGTT GTCTGGCAAT TCTGCTAATC ATCATGCTGA TAATCCTAGA 
CATGGCTCAA GTGACGACTA CCTACACATG GTGCACAGGA TAAGTAGTGA CGATGGAGAT 
TCTTCAACAA TGAGGAATGC TGCGTCTTTT CCCTTGAGGT CTCCACAGCC AGTATGTTCC 
CCTGCTGGAA GTGATGGGAC TCCAAAAGGA TCAAGACCAT CTTTAATCCT GCAGTCTCAG 
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGACA TCTTGTTAGA TTCTCCAGAA 
AGAAAGCAAA AGAAGCAAAA GAAAATGAAA TTAGGCAAAG ATGAAAAAGA CCAGAATGAG 
AAGGCTGCAA TGTATGATAT CATTAGCTCT CCATCCAAGG ACTCCACTAA ACTTACGTTA 
AGACTTACTC GTGTAAGATC TTCAGACATG GACCAGCAAG AGGATATGCT TTCTGGTATG 
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT 
TCAAAAACAC CCATTACTCC ACAGGATGTA AACCGCCCAC TAAATGCTGC TCAGTGTTTG 
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC 
ACTTCAGTTG CTACAAAACA GCCCCAGACT TCTGTGGTAC TGAATCAGCA ACAGGTATCA 
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA 
GAGAGAGAAT CAGCTATTGA AAGGGAGCGT TTTTCAAAAG AAGTTCAAGA TAAAGATAAG 
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT 
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG 
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCCTCTAT AACTCAGGAT 
TCAGACTCCA TTAAAAAGCC TGAAGAAATC AAACAATGTA ACGACACACC TATTTCTGTT 
CTTCAGGAAG ATATTATTGG AAATCTTAAA TCTACACCAG AAAACCATCC TGAGACTCCT 
AAAAAAAAGT CTGATCCTGA GCTTTTAAAG AGTGAAGTGA AACAGAATGA AAGTAGATTG 
GCAGAGTCTA AACCAAATGA AAACAGATTG GTAGAGACAA AATCTAGTGA AACTAAGTTA 
GAAACTAAAA TTGAGGTACC AACAGAAGAA CCTAAACAGA ATGAGAACAG AATGATTGAA 
TCCAGACAAA ATGAGAGCAG CACAGCCGAG CCTAAACAAA ATGAAAATAG ACTGCCGGAC 
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGACCC 
GAAACCCCAA AGCAAAAGGG TGAAAGCCGA CCTGAAACTC CAAAACAAAA GAGTGATGGG 
CGTCCTGAAA CCCCCAAACA AAAGGGTGAT GGACGGCCTG AAACTCCAAA GCAGAAAGGT 
GAGGGCCGAC CTGAAACTCC AAAGCAAAAA AATGAAGGTC GGCCTGAAAC ACCGAAACAT 
AGACATGAAA ATAGGAGGGA TTCTGGAAAG CCATCAACAG AGAAAAAACC TGAAGTGTCT 
AAACATAAAC AGGACATTAA ATCTGATTCA CCTCGGTTAA AATCAGAAAG AGCTGAAGCC 
TTAAAGCAGA GACCTGATGG GCGATCCATC TCTGAGTCAC TAAGACGTGA TCATGACAGT 
AAACAAAAGT CAGATGACAG GAGTGAATCT GAACGACATC GAGGGGATCA ATCTAGGGTT 
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGACC ATGGCATTAA ATCTGATGGT 
TCAAAACCTG ATAAACTAGA AAGAAAACAC AGACATGAAT CAGGGGACTC AAGGGAAAGA 
CCATCTTCTG GGGAACAAAA GTCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT 
AATAAATCAA GACCTGATAA GCCTGGTTTT AAATCTCCAA ATAGTAAGGA TGACAGAAGG 
ACAGAGGGTA ACAAGAGTAA AGTAGACAGT AATAAAACGC ACCCTGATAA TAAGGCAGAA 
TTTCCAAGTT ATTTGCTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCAAAA 
ATCAAGAGGG ATAAAGATGG CAATATTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA 
GAGCAGAAAG ACAAAGTAGA AAAAATGGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG 
CCTGTAGTTG TCCTGCAAAA ACTGTCTTTG GATGATGTTC AAAAACTTAT TAAAGATAGA 
GAGGATAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA 
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAA 
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAATAAAC GCAAGCGTAG CACAGTTAAT 
GAAAAACCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAACTTTT 
GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT 
GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGAAGT 
TCAGGTGGTG GTCGTTATCG AAACCGAAGC CCATCAGATT CTGACATGGA AGATTATTCT 
CCTCCTCCCA GCCTTAGTGA GGTCGCTAGA AAAATGAAGA AAAAAGAAAA ACAGAAGAAG 
CGGAAAGCTT ATGAACCAAA ACTAACACCC GAAGAAATGA TGGATTCTTC AACTTTTAAG 
AGATTCACAG CCTCAATAGA GAATATTTTG GATAACTTAG AAGATATGGA TTTTACTGCA 
TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA 
CTTGGCAGTG AATCTGCTAA AATTAAAGCA ATGGGTATAA TGGATAAGCT TTCCACTGAC 
AAAACTGTGA AAGTCCTAAA TATCTTGGAA AAGAATATTC AAGATGGGTC AAAGCTCTCC 
ACTTTGTTAA ATCATAATAA TGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT 
ATGGAGAGAG TGACAAAATC AGCAGATGCC TGTCTTACAA CTATAAACAT CATGACATCC 
CCTAACATGC CAAAAGCTGT ATATATTGAG GATGTAATCG AAAGAGTTAT ACAGTACACT 
AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT 
CATGGAGGAG GCTTACTAAG TTCAAAGGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG 
AGAGTAATAG TAATGCTTTA TAATAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAACTA 
CTAGAAATAC AACTTCTAAC AGACACAACA ATCCTTCAGG TATCTTCAAT GGGAATAACA 
CCATTTTTTG TGGAAAATGT CAGTGAACTA CAATTGTGTG CCATTAAATT AGTTACTGCA 
GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTG 
GCAAGATTAC CTACCAGCAA GAGGAGCTTA AGGAACTTCA GGTTAAATAG TAGTGATATG 
GATGGAGAGC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT AATTCAGTGT 
GTAGTACACT TACCATCATC AGAGAAGGAC TCTAATTCAG AAGAGGACTC AAATAAAAAA 
GTTGATCAAG ATGTTATCAT TACCAATTCT TATGAAACAG CCATGCGAAC AGCCCAAAAC 
TTCCTTTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA 
CTATTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT 
GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAATAAGTCA 
ACAGAGATGG CTTTAAGAGT GGCATCCCTT GATTACCTTG GAACTGTCGC TGCACGACTG 
AGGAAAGATG CTGTTACAAG CAAAATGGAT CAAGGGTCTA TAGAACGAAT TTTAAAACAG 
GTTTCAGGAG GGGAAGATGA AATACAGCAA TTACAAAAAG CATTGCTTGA TTACTTGGAT 
GAAAACACGG AGACTGATCC TTCACTGGTG TTTTCTCGTA AATTCTATAT TGCCCAGTGG 
TTCCGTGATA CAACACTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA 
TCTGAAGGAT CACATCATGC AAAGGAAGTT GAGACAACTG GTCAAATTAT GCATCGAGCT 
GAAAACCGGA AGAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTCAGCACA 
TTAAAAATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GTTTGATTGT TCGATACTTG 
GCCTCTATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTTCGAGTC 
CTTGGTGAAA ATGCAATTGC CGTTCGAACA AAAGCCATGA AGTGTTTATC TGAGGTTGTT 
GCTGTAGACC CCAGTATTCT AGCAAGGCTG GATATGCAGC GAGGAGTTCA TGGACGATTA 
ATGGATAACT CCACTAGTGT CAGAGAAGCA GCAGTGGAAT TACTAGGTCG ATTTGTCCTT 
TGTCGACCTC AGCTTGCTGA GCAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT 
GGTATCAGTG TCAGAAAAAG GGTGATAAAG ATTCTAAGAG ACATCTGTAT TGAACAACCG 
ACATTTCCAA AAATTACAGA AATGTGTGTA AAAATGATTC GCAGGGTAAA TGATGAAGAG 
GGCATTAAGA AATTAGTAAA TGAAACATTC CAAAAACTCT GGTTTACTCC AACTCCACAC 
AATGACAAAG AAGCAATGAC AAGGAAGATT TTAAATATCA CTGATGTGGT TGCAGCATGC 
AGAGATACTG GATATGACTG GTTTGAGCAG CTTCTTCAAA ATTTGTTGAA GTCTGAAGAG 
GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG 
CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA 
AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG 
GTTAAACATG CCATGACTAT GCAGCCATAT CTTACCACTA AATGTAGTAC ACAAAATGAT 
TTCATGGTTA TCTGCAATGT TGCAAAAATT CTAGAACTAG TTGTACCACT GATGGAACAT 
CCGAGTGAAA CTTTCCTTGC CACTATTGAA GAAGATCTAA TGAAGCTTAT CATCAAATAT 
GGCATGACTG TAGTGCAACA TTGTGTAAGC TGTCTTGGGG CTGTTGTAAA TAAAGTGACA 
CAAAATTTTA AATTTGTGTG GGCCTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA 
AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTGC TAACAAACAA ACCAGCACTT 
TTGAGATCCC TATTCACTGT TGGGGCACTG TGTCGGCATT TTGATTTTGA TCTGGAAGAT 
TTTAAAGGCA ACAGTAAGGT TAACATAAAG GATAAAGTAC TTGAACTATT GATGTATTTT 
ACAAAGCATT CAGATGAAGA AGTGCAAACA AAAGCCATCA TTGGTCTAGG ATTTGCCTTT 
ATTCAGCACC CAAGTCTAAT GTTTGAGCAA GAGGTGAAAA ATCTATACAA TAATATTTTA 
TCTGATAAAA ACTCCTCAGT AAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC 
CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA GGTTGCAAAA 
CAGGAAGATT TGAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG 
CTTTACCTCA AACAGGTGCT TGAGGCGTTT TTTCACACCC AATCAAGTGT ACGCCACTTC 
GCCCTCAATG TCATTGCATT GACTCTAAAC CAAGGTCTTA TTCATCCAGT TCAGTGTGTG 
CCGTATTTAA TTGCTATGGG CACAGACCCA GAACCAGCTA TGCGGAACAA GGCTGATCAG 
CAGCTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ACATGAAAGC AGTGGCTGGC 
ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACGT GCCTAAAAGA TCCTGTAAGG 
GGTTTCCGGC AGGACGAGTC CTCCAGTGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT 
GGAAACCGCC AGCACAGACG CGCCTTTCTG ATTTCTTTAC TCAACCTCTT TGATGACACA 
GCAAAAACAG AAGTGAATAT GCTCTTGTAT ATAGCAGACA ATCTAGCCTG TTTTCCATAC 
CAGACACAGG AAGAGCCGTT GTTTATAATG CATCATATAG ACATTACACT CTCTGTTTCT 
GGTAGTAACC TACTGCAGTC ATTCAAGGAG TCCATGGTAA AAGACAAAAG GAAAGAGAGA 
AAATCATCTC CTGGTAAGGA AAATGAGTCA AGCGAGAGTG AAGAAGAAGT CTCTAGGCCT 
CGAAAGTCAC GGAAACGTGT AGATTCAGAT TCAGATTCGG ATTCAGAAGA TGATATAAAT 
TCGGTGATGA AATGTTTGCC AGAAAATTCA GCTCCTTTAA TTGAGTTTGC AAATGTCTCC 
CAGGGTATTT TATTGCTTCT GATGTTAAAG CAACATTTGA AGAATCTCTG TGGATTTTCT 
GATAGTAAAA TTCAGAAATA CTCTCCATCC GAATCTGCAA AAGTGTATGA TAAAGCGATA 
AACCGGAAAA CAGGAGTTCA TTTTCATCCC AAGCAAACAC TGGACTTCCT GCGGAGTGAT 
ATGGCTAATT CCAAAATCAC TGAAGATGTG AAGAGGAGTA TAGTAAAACA GTACCTAGAT 
TTCAAACTTC TCATGGAACA TCTGGACCCT GATGAAGAGG AAGAAGAAGG GGAGGTGTCA 
GCCAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCGCTGC TTGGAGGAGG CAGCCCTAAA 
AATAATGCAG CAGCAGAGAC AGAAGATGAT GAAAGTGATG GGGAGGATAG AGGAGGAGGC 
ACTTCAGGGT CATTGAGAAG GTCAAAACGA AATTCAGACT CTACGGAGTT GGCAGCACAG 
ATGAATGAAA GTGTTGACGT CATGGATGTC ATCGCTATTT GCTGTCCAAA GTACAAAGAC 
CGACCACAGA TTGCAAGAGT AGTACAGAAA ACCAGCAGTG GCTTCAGTGT TCAGTGGATG 
GCAGGCTCCT ACAGTGGCTC CTGGACTGAG GCTAAGCGCC GTGATGGCCG CAAACTGGTG 
CCTTGGGTAG ACACTATTAA AGAGTCAGAC ATTATTTACA AAAAAATTGC TCTAACGAGT 
GCTAATAAGC TGACTAATAA AGTTGTTCAG ACTTTACGAT CCCTGTATGC CGCCAAGGAT 
GGGACTTCCA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001193513.1
CDS	502..8916
Translation	MNGDMPHVPITTLAGIASLTDLLNQLPLPSPLPATTTKSLLFNARIAEEVNCLLACRDDNLVSQLVHSLNQVSTDHIELKDNLGSDDPEGDIPVLLQAVLARSPNVFREKSMQNRYVQSGMMMSQYKLSQNSMHSSPASSNYQQTTISHSPSSRFVPPQTSSGNRFMPQQNSPVPSPYAPQSPAGYMPYSHPSSYTTHPQMQQASVSSPIVAGGLRNLHDNKVSGSLSGNSANHHADNPRHGSSDDYLHMVHRISSDDGDSSTMRNAASFPLRSPQPVCSPAGSDGTPKGSRPSLILQSQSLPCSSPRDVPPDILLDSPERKQKKQKKMKLGKDEKDQNEKAAMYDIISSPSKDSTKLTLRLTRVRSSDMDQQEDMLSGMENSNVSENDIPFNVQYPGQTSKTPITPQDVNRPLNAAQCLSQQEQTAFLPANQVPVLQQNTSVATKQPQTSVVLNQQQVSQQGPIYDEVELDALAEIERIERESAIERERFSKEVQDKDKPLKKRKQDSYPQEAGGATGGNRPASQETGSTGNGSRPALMVSIDLHQAGRVDSQASITQDSDSIKKPEEIKQCNDTPISVLQEDIIGNLKSTPENHPETPKKKSDPELLKSEVKQNESRLAESKPNENRLVETKSSETKLETKIEVPTEEPKQNENRMIESRQNESSTAEPKQNENRLPDTKPNDNKQNNGRSETTKSRPETPKQKGESRPETPKQKSDGRPETPKQKGDGRPETPKQKGEGRPETPKQKNEGRPETPKHRHENRRDSGKPSTEKKPEVSKHKQDIKSDSPRLKSERAEALKQRPDGRSISESLRRDHDSKQKSDDRSESERHRGDQSRVRRPETLRSSSRNDHGIKSDGSKPDKLERKHRHESGDSRERPSSGEQKSRPDSPRVKQGDSNKSRPDKPGFKSPNSKDDRRTEGNKSKVDSNKTHPDNKAEFPSYLLGGRSGALKNFVIPKIKRDKDGNITQETKKMEMKGEQKDKVEKMGLVEDLNKGAKPVVVLQKLSLDDVQKLIKDREDKSRSSLKPIKNKPSKSNKGSIDQSVLKELPPELLAEIESTMPLCERVKMNKRKRSTVNEKPKYAEISSDEDNDSDETFESSRKRHKKDDDKAWEYEERDRRSSGDHRRSGHSHEGRRSSGGGRYRNRSPSDSDMEDYSPPPSLSEVARKMKKKEKQKKRKAYEPKLTPEEMMDSSTFKRFTASIENILDNLEDMDFTAFGDDDEIPQELLLGKHQLNELGSESAKIKAMGIMDKLSTDKTVKVLNILEKNIQDGSKLSTLLNHNNDTEEEERLWRDLIMERVTKSADACLTTINIMTSPNMPKAVYIEDVIERVIQYTKFHLQNTLYPQYDPVYRLDPHGGGLLSSKAKRAKCSTHKQRVIVMLYNKVCDIVSSLSELLEIQLLTDTTILQVSSMGITPFFVENVSELQLCAIKLVTAVFSRYEKHRQLILEEIFTSLARLPTSKRSLRNFRLNSSDMDGEPMYIQMVTALVLQLIQCVVHLPSSEKDSNSEEDSNKKVDQDVIITNSYETAMRTAQNFLSIFLKKCGSKQGEEDYRPLFENFVQDLLSTVNKPEWPAAELLLSLLGRLLVHQFSNKSTEMALRVASLDYLGTVAARLRKDAVTSKMDQGSIERILKQVSGGEDEIQQLQKALLDYLDENTETDPSLVFSRKFYIAQWFRDTTLETEKAMKSQKDEESSEGSHHAKEVETTGQIMHRAENRKKFLRSIIKTTPSQFSTLKMNSDTVDYDDACLIVRYLASMRPFAQSFDIYLTQILRVLGENAIAVRTKAMKCLSEVVAVDPSILARLDMQRGVHGRLMDNSTSVREAAVELLGRFVLCRPQLAEQYYDMLIERILDTGISVRKRVIKILRDICIEQPTFPKITEMCVKMIRRVNDEEGIKKLVNETFQKLWFTPTPHNDKEAMTRKILNITDVVAACRDTGYDWFEQLLQNLLKSEEDSSYKPVKKACTQLVDNLVEHILKYEESLADSDNKGVNSGRLVACITTLFLFSKIRPQLMVKHAMTMQPYLTTKCSTQNDFMVICNVAKILELVVPLMEHPSETFLATIEEDLMKLIIKYGMTVVQHCVSCLGAVVNKVTQNFKFVWACFNRYYGAISKLKSQHQEDPNNTSLLTNKPALLRSLFTVGALCRHFDFDLEDFKGNSKVNIKDKVLELLMYFTKHSDEEVQTKAIIGLGFAFIQHPSLMFEQEVKNLYNNILSDKNSSVNLKIQVLKNLQTYLQEEDTRMQQADRDWKKVAKQEDLKEMGDVSSGMSSSIMQLYLKQVLEAFFHTQSSVRHFALNVIALTLNQGLIHPVQCVPYLIAMGTDPEPAMRNKADQQLVEIDKKYAGFIHMKAVAGMKMSYQVQQAINTCLKDPVRGFRQDESSSALCSHLYSMIRGNRQHRRAFLISLLNLFDDTAKTEVNMLLYIADNLACFPYQTQEEPLFIMHHIDITLSVSGSNLLQSFKESMVKDKRKERKSSPGKENESSESEEEVSRPRKSRKRVDSDSDSDSEDDINSVMKCLPENSAPLIEFANVSQGILLLLMLKQHLKNLCGFSDSKIQKYSPSESAKVYDKAINRKTGVHFHPKQTLDFLRSDMANSKITEDVKRSIVKQYLDFKLLMEHLDPDEEEEEGEVSASTNARNKAITSLLGGGSPKNNAAAETEDDESDGEDRGGGTSGSLRRSKRNSDSTELAAQMNESVDVMDVIAICCPKYKDRPQIARVVQKTSSGFSVQWMAGSYSGSWTEAKRRDGRKLVPWVDTIKESDIIYKKIALTSANKLTNKVVQTLRSLYAAKDGTSS

Target ORF information:

RefSeq Version	NM_001206584.1
Organism	Bos taurus(cattle)
Definition	Bos taurus NIPBL, cohesin loading factor (NIPBL), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001206584.1

ORF Insert Sequence:

ATGAATGGGG ATATGCCCCA TGTCCCCATC ACTACTCTTG CGGGGATTGC TAGTCTTACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCC CCTTTACCTG CTACAACTAC AAAGAGCCTT 
CTCTTTAATG CACGAATAGC AGAAGAAGTG AACTGCCTTT TGGCCTGTAG GGATGATAAT 
TTGGTTTCAC AGCTTGTCCA CAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA 
GATAACCTTG GCAGTGATGA TCCAGAAGGC GACATACCAG TCTTGTTGCA GGCCGTCCTG 
GCGAGGAGTC CTAACGTTTT CAGGGAGAAA AGTATGCAGA ACAGATATGT ACAAAGTGGA 
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC 
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA 
AGCTCTGGGA ACAGATTTAT GCCACAACAA AATAGCCCAG TGCCTAGTCC ATATGCCCCA 
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTATACAAC ACATCCACAG 
ATGCAGCAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CCTACATGAT 
AATAAAGTTT CTGGTTCGTT GTCTGGCAAT TCTGCTAATC ATCATGCTGA TAATCCTAGA 
CATGGCTCAA GTGACGACTA CCTACACATG GTGCACAGGA TAAGTAGTGA CGATGGAGAT 
TCTTCAACAA TGAGGAATGC TGCGTCTTTT CCCTTGAGGT CTCCACAGCC AGTATGTTCC 
CCTGCTGGAA GTGATGGGAC TCCAAAAGGA TCAAGACCAT CTTTAATCCT GCAGTCTCAG 
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGACA TCTTGTTAGA TTCTCCAGAA 
AGAAAGCAAA AGAAGCAAAA GAAAATGAAA TTAGGCAAAG ATGAAAAAGA CCAGAATGAG 
AAGGCTGCAA TGTATGATAT CATTAGCTCT CCATCCAAGG ACTCCACTAA ACTTACGTTA 
AGACTTACTC GTGTAAGATC TTCAGACATG GACCAGCAAG AGGATATGCT TTCTGGTATG 
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT 
TCAAAAACAC CCATTACTCC ACAGGATGTA AACCGCCCAC TAAATGCTGC TCAGTGTTTG 
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC 
ACTTCAGTTG CTACAAAACA GCCCCAGACT TCTGTGGTAC TGAATCAGCA ACAGGTATCA 
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA 
GAGAGAGAAT CAGCTATTGA AAGGGAGCGT TTTTCAAAAG AAGTTCAAGA TAAAGATAAG 
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT 
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG 
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCCTCTAT AACTCAGGAT 
TCAGACTCCA TTAAAAAGCC TGAAGAAATC AAACAATGTA ACGACACACC TATTTCTGTT 
CTTCAGGAAG ATATTATTGG AAATCTTAAA TCTACACCAG AAAACCATCC TGAGACTCCT 
AAAAAAAAGT CTGATCCTGA GCTTTTAAAG AGTGAAGTGA AACAGAATGA AAGTAGATTG 
GCAGAGTCTA AACCAAATGA AAACAGATTG GTAGAGACAA AATCTAGTGA AACTAAGTTA 
GAAACTAAAA TTGAGGTACC AACAGAAGAA CCTAAACAGA ATGAGAACAG AATGATTGAA 
TCCAGACAAA ATGAGAGCAG CACAGCCGAG CCTAAACAAA ATGAAAATAG ACTGCCGGAC 
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGACCC 
GAAACCCCAA AGCAAAAGGG TGAAAGCCGA CCTGAAACTC CAAAACAAAA GAGTGATGGG 
CGTCCTGAAA CCCCCAAACA AAAGGGTGAT GGACGGCCTG AAACTCCAAA GCAGAAAGGT 
GAGGGCCGAC CTGAAACTCC AAAGCAAAAA AATGAAGGTC GGCCTGAAAC ACCGAAACAT 
AGACATGAAA ATAGGAGGGA TTCTGGAAAG CCATCAACAG AGAAAAAACC TGAAGTGTCT 
AAACATAAAC AGGACATTAA ATCTGATTCA CCTCGGTTAA AATCAGAAAG AGCTGAAGCC 
TTAAAGCAGA GACCTGATGG GCGATCCATC TCTGAGTCAC TAAGACGTGA TCATGACAGT 
AAACAAAAGT CAGATGACAG GAGTGAATCT GAACGACATC GAGGGGATCA ATCTAGGGTT 
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGACC ATGGCATTAA ATCTGATGGT 
TCAAAACCTG ATAAACTAGA AAGAAAACAC AGACATGAAT CAGGGGACTC AAGGGAAAGA 
CCATCTTCTG GGGAACAAAA GTCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT 
AATAAATCAA GACCTGATAA GCCTGGTTTT AAATCTCCAA ATAGTAAGGA TGACAGAAGG 
ACAGAGGGTA ACAAGAGTAA AGTAGACAGT AATAAAACGC ACCCTGATAA TAAGGCAGAA 
TTTCCAAGTT ATTTGCTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCAAAA 
ATCAAGAGGG ATAAAGATGG CAATATTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA 
GAGCAGAAAG ACAAAGTAGA AAAAATGGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG 
CCTGTAGTTG TCCTGCAAAA ACTGTCTTTG GATGATGTTC AAAAACTTAT TAAAGATAGA 
GAGGATAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA 
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAA 
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAATAAAC GCAAGCGTAG CACAGTTAAT 
GAAAAACCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAACTTTT 
GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT 
GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGAAGT 
TCAGGTGGTG GTCGTTATCG AAACCGAAGC CCATCAGATT CTGACATGGA AGATTATTCT 
CCTCCTCCCA GCCTTAGTGA GGTCGCTAGA AAAATGAAGA AAAAAGAAAA ACAGAAGAAG 
CGGAAAGCTT ATGAACCAAA ACTAACACCC GAAGAAATGA TGGATTCTTC AACTTTTAAG 
AGATTCACAG CCTCAATAGA GAATATTTTG GATAACTTAG AAGATATGGA TTTTACTGCA 
TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA 
CTTGGCAGTG AATCTGCTAA AATTAAAGCA ATGGGTATAA TGGATAAGCT TTCCACTGAC 
AAAACTGTGA AAGTCCTAAA TATCTTGGAA AAGAATATTC AAGATGGGTC AAAGCTCTCC 
ACTTTGTTAA ATCATAATAA TGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT 
ATGGAGAGAG TGACAAAATC AGCAGATGCC TGTCTTACAA CTATAAACAT CATGACATCC 
CCTAACATGC CAAAAGCTGT ATATATTGAG GATGTAATCG AAAGAGTTAT ACAGTACACT 
AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT 
CATGGAGGAG GCTTACTAAG TTCAAAGGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG 
AGAGTAATAG TAATGCTTTA TAATAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAACTA 
CTAGAAATAC AACTTCTAAC AGACACAACA ATCCTTCAGG TATCTTCAAT GGGAATAACA 
CCATTTTTTG TGGAAAATGT CAGTGAACTA CAATTGTGTG CCATTAAATT AGTTACTGCA 
GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTG 
GCAAGATTAC CTACCAGCAA GAGGAGCTTA AGGAACTTCA GGTTAAATAG TAGTGATATG 
GATGGAGAGC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT AATTCAGTGT 
GTAGTACACT TACCATCATC AGAGAAGGAC TCTAATTCAG AAGAGGACTC AAATAAAAAA 
GTTGATCAAG ATGTTATCAT TACCAATTCT TATGAAACAG CCATGCGAAC AGCCCAAAAC 
TTCCTTTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA 
CTATTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT 
GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAATAAGTCA 
ACAGAGATGG CTTTAAGAGT GGCATCCCTT GATTACCTTG GAACTGTCGC TGCACGACTG 
AGGAAAGATG CTGTTACAAG CAAAATGGAT CAAGGGTCTA TAGAACGAAT TTTAAAACAG 
GTTTCAGGAG GGGAAGATGA AATACAGCAA TTACAAAAAG CATTGCTTGA TTACTTGGAT 
GAAAACACGG AGACTGATCC TTCACTGGTG TTTTCTCGTA AATTCTATAT TGCCCAGTGG 
TTCCGTGATA CAACACTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA 
TCTGAAGGAT CACATCATGC AAAGGAAGTT GAGACAACTG GTCAAATTAT GCATCGAGCT 
GAAAACCGGA AGAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTCAGCACA 
TTAAAAATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GTTTGATTGT TCGATACTTG 
GCCTCTATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTTCGAGTC 
CTTGGTGAAA ATGCAATTGC CGTTCGAACA AAAGCCATGA AGTGTTTATC TGAGGTTGTT 
GCTGTAGACC CCAGTATTCT AGCAAGGCTG GATATGCAGC GAGGAGTTCA TGGACGATTA 
ATGGATAACT CCACTAGTGT CAGAGAAGCA GCAGTGGAAT TACTAGGTCG ATTTGTCCTT 
TGTCGACCTC AGCTTGCTGA GCAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT 
GGTATCAGTG TCAGAAAAAG GGTGATAAAG ATTCTAAGAG ACATCTGTAT TGAACAACCG 
ACATTTCCAA AAATTACAGA AATGTGTGTA AAAATGATTC GCAGGGTAAA TGATGAAGAG 
GGCATTAAGA AATTAGTAAA TGAAACATTC CAAAAACTCT GGTTTACTCC AACTCCACAC 
AATGACAAAG AAGCAATGAC AAGGAAGATT TTAAATATCA CTGATGTGGT TGCAGCATGC 
AGAGATACTG GATATGACTG GTTTGAGCAG CTTCTTCAAA ATTTGTTGAA GTCTGAAGAG 
GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG 
CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA 
AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG 
GTTAAACATG CCATGACTAT GCAGCCATAT CTTACCACTA AATGTAGTAC ACAAAATGAT 
TTCATGGTTA TCTGCAATGT TGCAAAAATT CTAGAACTAG TTGTACCACT GATGGAACAT 
CCGAGTGAAA CTTTCCTTGC CACTATTGAA GAAGATCTAA TGAAGCTTAT CATCAAATAT 
GGCATGACTG TAGTGCAACA TTGTGTAAGC TGTCTTGGGG CTGTTGTAAA TAAAGTGACA 
CAAAATTTTA AATTTGTGTG GGCCTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA 
AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTGC TAACAAACAA ACCAGCACTT 
TTGAGATCCC TATTCACTGT TGGGGCACTG TGTCGGCATT TTGATTTTGA TCTGGAAGAT 
TTTAAAGGCA ACAGTAAGGT TAACATAAAG GATAAAGTAC TTGAACTATT GATGTATTTT 
ACAAAGCATT CAGATGAAGA AGTGCAAACA AAAGCCATCA TTGGTCTAGG ATTTGCCTTT 
ATTCAGCACC CAAGTCTAAT GTTTGAGCAA GAGGTGAAAA ATCTATACAA TAATATTTTA 
TCTGATAAAA ACTCCTCAGT AAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC 
CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA GGTTGCAAAA 
CAGGAAGATT TGAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG 
CTTTACCTCA AACAGGTGCT TGAGGCGTTT TTTCACACCC AATCAAGTGT ACGCCACTTC 
GCCCTCAATG TCATTGCATT GACTCTAAAC CAAGGTCTTA TTCATCCAGT TCAGTGTGTG 
CCGTATTTAA TTGCTATGGG CACAGACCCA GAACCAGCTA TGCGGAACAA GGCTGATCAG 
CAGCTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ACATGAAAGC AGTGGCTGGC 
ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACGT GCCTAAAAGA TCCTGTAAGG 
GGTTTCCGGC AGGACGAGTC CTCCAGTGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT 
GGAAACCGCC AGCACAGACG CGCCTTTCTG ATTTCTTTAC TCAACCTCTT TGATGACACA 
GCAAAAACAG AAGTGAATAT GCTCTTGTAT ATAGCAGACA ATCTAGCCTG TTTTCCATAC 
CAGACACAGG AAGAGCCGTT GTTTATAATG CATCATATAG ACATTACACT CTCTGTTTCT 
GGTAGTAACC TACTGCAGTC ATTCAAGGAG TCCATGGTAA AAGACAAAAG GAAAGAGAGA 
AAATCATCTC CTGGTAAGGA AAATGAGTCA AGCGAGAGTG AAGAAGAAGT CTCTAGGCCT 
CGAAAGTCAC GGAAACGTGT AGATTCAGAT TCAGATTCGG ATTCAGAAGA TGATATAAAT 
TCGGTGATGA AATGTTTGCC AGAAAATTCA GCTCCTTTAA TTGAGTTTGC AAATGTCTCC 
CAGGGTATTT TATTGCTTCT GATGTTAAAG CAACATTTGA AGAATCTCTG TGGATTTTCT 
GATAGTAAAA TTCAGAAATA CTCTCCATCC GAATCTGCAA AAGTGTATGA TAAAGCGATA 
AACCGGAAAA CAGGAGTTCA TTTTCATCCC AAGCAAACAC TGGACTTCCT GCGGAGTGAT 
ATGGCTAATT CCAAAATCAC TGAAGATGTG AAGAGGAGTA TAGTAAAACA GTACCTAGAT 
TTCAAACTTC TCATGGAACA TCTGGACCCT GATGAAGAGG AAGAAGAAGG GGAGGTGTCA 
GCCAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCGCTGC TTGGAGGAGG CAGCCCTAAA 
AATAATGCAG CAGCAGAGAC AGAAGATGAT GAAAGTGATG GGGAGGATAG AGGAGGAGGC 
ACTTCAGGGT CATTGAGAAG GTCAAAACGA AATTCAGACT CTACGGAGTT GGCAGCACAG 
ATGAATGAAA GTGTTGACGT CATGGATGTC ATCGCTATTT GCTGTCCAAA GTACAAAGAC 
CGACCACAGA TTGCAAGAGT AGTACAGAAA ACCAGCAGTG GCTTCAGTGT TCAGTGGATG 
GCAGGCTCCT ACAGTGGCTC CTGGACTGAG GCTAAGCGCC GTGATGGCCG CAAACTGGTG 
CCTTGGGTAG ACACTATTAA AGAGTCAGAC ATTATTTACA AAAAAATTGC TCTAACGAGT 
GCTAATAAGC TGACTAATAA AGTTGTTCAG ACTTTACGAT CCCTGTATGC CGCCAAGGAT 
GGGACTTCCA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIPBL ( XM_024981354.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024837122.1 Bos taurus NIPBL, cohesin loading factor (NIPBL), transcript variant X1, mRNA.

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CloneID	OBa228057
Clone ID Related Accession (Same CDS sequence)	XM_024981354.1
Accession Version	XM_024981354.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8169bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	nipped-B-like protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGGG ATATGCCCCA TGTCCCCATC ACTACTCTTG CGGGGATTGC TAGTCTTACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCC CCTTTACCTG CTACAACTAC AAAGAGCCTT 
CTCTTTAATG CACGAATAGC AGAAGAAGTG AACTGCCTTT TGGCCTGTAG GGATGATAAT 
TTGGTTTCAC AGCTTGTCCA CAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA 
GATAACCTTG GCAGTGATGA TCCAGAAGGC GACATACCAG TCTTGTTGCA GGCCGTCCTG 
GCGAGGAGTC CTAACGTTTT CAGGGAGAAA AGTATGCAGA ACAGATATGT ACAAAGTGGA 
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC 
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA 
AGCTCTGGGA ACAGATTTAT GCCACAACAA AATAGCCCAG TGCCTAGTCC ATATGCCCCA 
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTATACAAC ACATCCACAG 
ATGCAGCAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CCTACATGAT 
AATAAAGTTT CTGGTTCGTT GTCTGGCAAT TCTGCTAATC ATCATGCTGA TAATCCTAGA 
CATGGCTCAA GTGACGACTA CCTACACATG GTGCACAGGA TAAGTAGTGA CGATGGAGAT 
TCTTCAACAA TGAGGAATGC TGCGTCTTTT CCCTTGAGGT CTCCACAGCC AGTATGTTCC 
CCTGCTGGAA GTGATGGGAC TCCAAAAGGA TCAAGACCAT CTTTAATCCT GCAGTCTCAG 
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGACA TCTTGTTAGA TTCTCCAGAA 
AGAAAGCAAA AGAAGCAAAA GAAAATGAAA TTAGGCAAAG ATGAAAAAGA CCAGAATGAG 
AAGGCTGCAA TGTATGATAT CATTAGCTCT CCATCCAAGG ACTCCACTAA ACTTACGTTA 
AGACTTACTC GTGTAAGATC TTCAGACATG GACCAGCAAG AGGATATGCT TTCTGGTATG 
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT 
TCAAAAACAC CCATTACTCC ACAGGATGTA AACCGCCCAC TAAATGCTGC TCAGTGTTTG 
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC 
ACTTCAGTTG CTACAAAACA GCCCCAGACT TCTGTGGTAC TGAATCAGCA ACAGGTATCA 
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA 
GAGAGAGAAT CAGCTATTGA AAGGGAGCGT TTTTCAAAAG AAGTTCAAGA TAAAGATAAG 
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT 
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG 
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCCTCTAT AACTCAGGAT 
TCAGACTCCA TTAAAAAGCC TGAAGAAATC AAACAATGTA ATGACACACC TATTTCTGTT 
CTTCAGGAAG ATATTATTGG AAATCTTAAA TCTACACCAG AAAACCATCC TGAGACTCCT 
AAAAAAAAGT CTGATCCTGA GCTTTTAAAG AGTGAAGTGA AACAGAATGA AAGTAGATTG 
GCAGAGTCTA AACCAAATGA AAACAGGTTG GTAGAGACAA AATCTAGTGA AACTAAGTTA 
GAAACTAAAA TTGAGGTACC AACAGAAGAA CCTAAACAGA ATGAGAACAG AATGATTGAA 
TCCAGACAAA ATGAGAGCAG CACAGCCGAG CCTAAACAAA ATGAAAATAG ACTGCCGGAC 
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGACCC 
GAAACCCCAA AGCAAAAGGG TGAAAGCCGA CCTGAAACTC CAAAACAAAA GAGTGATGGG 
CGTCCTGAAA CCCCCAAACA AAAGGGTGAT GGACGGCCTG AAACTCCAAA GCAGAAAGGT 
GAGGGCCGAC CTGAAACTCC AAAGCAAAAA AATGAAGGTC GGCCTGAAAC ACCGAAACAT 
AGACATGAAA ATAGGAGGGA TTCTGGAAAG CCATCAACAG AGAAAAAACC TGAAGTGTCT 
AAACATAAAC AGGACATTAA ATCTGATTCA CCTCGGTTAA AATCAGAAAG AGCTGAAGCC 
TTAAAGCAGA GACCTGATGG GCGATCCATC TCTGAGTCAC TAAGACGTGA TCATGACAGT 
AAACAAAAGT CAGATGACAG GAGTGAATCT GAACGACATC GAGGGGATCA ATCTAGGGTT 
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGACC ATGGCATTAA ATCTGATGGT 
TCAAAACCTG ATAAACTAGA AAGAAAACAC AGACATGAAT CAGGGGACTC AAGGGAAAGA 
CCATCTTCTG GGGAACAAAA GTCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT 
AATAAATCAA GACCTGATAA GCCTGGTTTT AAATCTCCAA ATAGTAAGGA TGACAGAAGG 
ACAGAGGGTA ACAAGAGTAA AGTAGACAGT AATAAAACGC ACCCTGATAA TAAGGCAGAA 
TTTCCAAGTT ATTTGCTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCAAAA 
ATCAAGAGGG ATAAAGATGG CAATATTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA 
GAGCAGAAAG ACAAAGTAGA AAAAATGGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG 
CCTGTAGTTG TCCTGCAAAA ACTGTCTTTG GATGATGTTC AAAAACTTAT TAAAGATAGA 
GAGGATAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA 
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAA 
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAATAAAC GCAAGCGTAG CACAGTTAAT 
GAAAAACCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAACTTTT 
GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT 
GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGAAGT 
TCAGGTGGTG GTCGTTATCG AAACCGAAGC CCATCAGATT CTGACATGGA AGATTATTCT 
CCTCCTCCCA GCCTTAGTGA GGTCGCTAGA AAAATGAAGA AAAAAGAAAA ACAGAAGAAG 
CGGAAAGCTT ATGAACCAAA ACTAACACCC GAAGAAATGA TGGATTCTTC AACTTTTAAG 
AGATTCACAG CCTCAATAGA GAATATTTTG GATAACTTAG AAGATATGGA TTTTACTGCA 
TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA 
CTTGGCAGTG AATCTGCTAA AATTAAAGCA ATGGGTATAA TGGATAAGCT TTCCACTGAC 
AAAACTGTGA AAGTCCTAAA TATCTTGGAA AAGAATATTC AAGATGGGTC AAAGCTCTCC 
ACTTTGTTAA ATCATAATAA TGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT 
ATGGAGAGAG TGACAAAATC AGCAGATGCC TGTCTTACAA CTATAAACAT CATGACATCC 
CCTAACATGC CAAAAGCTGT ATATATTGAG GATGTAATCG AAAGAGTTAT ACAGTACACT 
AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT 
CATGGAGGAG GCTTACTAAG TTCAAAGGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG 
AGAGTAATAG TAATGCTTTA TAATAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAACTA 
CTAGAAATAC AACTTCTAAC AGACACAACA ATCCTTCAGG TATCTTCAAT GGGAATAACA 
CCATTTTTTG TGGAAAATGT CAGTGAACTA CAATTGTGTG CCATTAAATT AGTTACTGCA 
GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTG 
GCAAGATTAC CTACCAGCAA GAGGAGCTTA AGGAACTTCA GGTTAAATAG TAGTGATATG 
GATGGAGAGC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT AATTCAGTGT 
GTAGTACACT TACCATCATC AGAGAAGGAC TCTAATTCAG AAGAGGACTC AAATAAAAAA 
GTTGATCAAG ATGTTATCAT TACCAATTCT TATGAAACAG CCATGCGAAC AGCCCAAAAC 
TTCCTTTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA 
CTATTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT 
GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAATAAGTCA 
ACAGAGATGG CTTTAAGAGT GGCATCCCTT GATTACCTTG GAACTGTCGC TGCACGACTG 
AGGAAAGATG CTGTTACAAG CAAAATGGAT CAAGGGTCTA TAGAACGAAT TTTAAAACAG 
GTTTCAGGAG GGGAAGATGA AATACAGCAA TTACAAAAAG CATTGCTTGA TTACTTGGAT 
GAAAACACGG AGACTGATCC TTCACTGGTG TTTTCTCGTA AATTCTATAT TGCCCAGTGG 
TTCCGTGATA CAACACTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA 
TCTGAAGGAT CACATCATGC AAAGGAAGTT GAGACAACTG GTCAAATTAT GCATCGAGCT 
GAAAACCGGA AGAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTCAGCACA 
TTAAAAATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GTTTGATTGT TCGATACTTG 
GCCTCTATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTTCGAGTC 
CTTGGTGAAA ATGCAATTGC CGTTCGAACA AAAGCCATGA AGTGTTTATC TGAGGTTGTT 
GCTGTAGACC CCAGTATTCT AGCAAGGCTG GATATGCAGC GAGGAGTTCA TGGACGATTA 
ATGGATAACT CCACTAGTGT CAGAGAAGCA GCAGTGGAAT TACTAGGTCG ATTTGTCCTT 
TGTCGACCTC AGCTTGCTGA GCAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT 
GGTATCAGTG TCAGAAAAAG GGTGATAAAG ATTCTAAGAG ACATCTGTAT TGAACAACCG 
ACATTTCCAA AAATTACAGA AATGTGTGTA AAAATGATTC GCAGGGTAAA TGATGAAGAG 
GGCATTAAGA AATTAGTAAA TGAAACATTC CAAAAACTCT GGTTTACTCC AACTCCACAC 
AATGACAAAG AAGCAATGAC AAGGAAGATT TTAAATATCA CTGATGTGGT TGCAGCATGC 
AGAGATACTG GATATGACTG GTTTGAGCAG CTTCTTCAAA ATACACAAAA TGATTTCATG 
GTTATCTGCA ATGTTGCAAA AATTCTAGAA CTAGTTGTAC CACTGATGGA ACATCCGAGT 
GAAACTTTCC TTGCCACTAT TGAAGAAGAT CTAATGAAGC TTATCATCAA ATATGGCATG 
ACTGTAGTGC AACATTGTGT AAGCTGTCTT GGGGCTGTTG TAAATAAAGT GACACAAAAT 
TTTAAATTTG TGTGGGCCTG TTTCAATAGA TACTATGGTG CCATTTCAAA ATTAAAAAGT 
CAACACCAAG AGGACCCAAA TAACACTTCA CTGCTAACAA ACAAACCAGC ACTTTTGAGA 
TCCCTATTCA CTGTTGGGGC ACTGTGTCGG CATTTTGATT TTGATCTGGA AGATTTTAAA 
GGCAACAGTA AGGTTAACAT AAAGGATAAA GTACTTGAAC TATTGATGTA TTTTACAAAG 
CATTCAGATG AAGAAGTGCA AACAAAAGCC ATCATTGGTC TAGGATTTGC CTTTATTCAG 
CACCCAAGTC TAATGTTTGA GCAAGAGGTG AAAAATCTAT ACAATAATAT TTTATCTGAT 
AAAAACTCCT CAGTAAATTT AAAAATACAA GTGTTAAAAA ACCTCCAGAC CTACCTACAA 
GAAGAAGATA CACGTATGCA GCAGGCAGAT AGAGACTGGA AGAAGGTTGC AAAACAGGAA 
GATTTGAAAG AAATGGGTGA TGTTTCCTCA GGGATGAGTA GTTCCATCAT GCAGCTTTAC 
CTCAAACAGG TGCTTGAGGC GTTTTTTCAC ACCCAATCAA GTGTACGCCA CTTCGCCCTC 
AATGTCATTG CATTGACTCT AAACCAAGGT CTTATTCATC CAGTTCAGTG TGTGCCGTAT 
TTAATTGCTA TGGGCACAGA CCCAGAACCA GCTATGCGGA ACAAGGCTGA TCAGCAGCTT 
GTGGAAATAG ACAAAAAATA TGCTGGATTC ATTCACATGA AAGCAGTGGC TGGCATGAAG 
ATGTCTTACC AGGTACAACA GGCAATCAAC ACGTGCCTAA AAGATCCTGT AAGGGGTTTC 
CGGCAGGACG AGTCCTCCAG TGCTTTGTGT TCACACCTTT ACTCCATGAT CCGTGGAAAC 
CGCCAGCACA GACGCGCCTT TCTGATTTCT TTACTCAACC TCTTTGATGA CACAGCAAAA 
ACAGAAGTGA ATATGCTCTT GTATATAGCA GACAATCTAG CCTGTTTTCC ATACCAGACA 
CAGGAAGAGC CGTTGTTTAT AATGCATCAT ATAGACATTA CACTCTCTGT TTCTGGTAGT 
AACCTACTGC AGTCATTCAA GGAGTCCATG GTAAAAGACA AAAGGAAAGA GAGAAAATCA 
TCTCCTGGTA AGGAAAATGA GTCAAGCGAG AGTGAAGAAG AAGTCTCTAG GCCTCGAAAG 
TCACGGAAAC GTGTAGATTC AGATTCAGAT TCGGATTCAG AAGATGATAT AAATTCGGTG 
ATGAAATGTT TGCCAGAAAA TTCAGCTCCT TTAATTGAGT TTGCAAATGT CTCCCAGGGT 
ATTTTATTGC TTCTGATGTT AAAGCAACAT TTGAAGAATC TCTGTGGATT TTCTGATAGT 
AAAATTCAGA AATACTCTCC ATCCGAATCT GCAAAAGTGT ATGATAAAGC GATAAACCGG 
AAAACAGGAG TTCATTTTCA TCCCAAGCAA ACACTGGACT TCCTGCGGAG TGATATGGCT 
AATTCCAAAA TCACTGAAGA TGTGAAGAGG AGTATAGTAA AACAGTACCT AGATTTCAAA 
CTTCTCATGG AACATCTGGA CCCTGATGAA GAGGAAGAAG AAGGGGAGGT GTCAGCCAGC 
ACAAATGCTC GGAACAAAGC AATTACCTCG CTGCTTGGAG GAGGCAGCCC TAAAAATAAT 
GCAGCAGCAG AGACAGAAGA TGATGAAAGT GATGGGGAGG ATAGAGGAGG AGGCACTTCA 
GGGTCATTGA GAAGGTCAAA ACGAAATTCA GACTCTACGG AGTTGGCAGC ACAGATGAAT 
GAAAGTGTTG ACGTCATGGA TGTCATCGCT ATTTGCTGTC CAAAGTACAA AGACCGACCA 
CAGATTGCAA GAGTAGTACA GAAAACCAGC AGTGGCTTCA GTGTTCAGTG GATGGCAGGC 
TCCTACAGTG GCTCCTGGAC TGAGGCTAAG CGCCGTGATG GCCGCAAACT GGTGCCTTGG 
GTAGACACTA TTAAAGAGTC AGACATTATT TACAAAAAAA TTGCTCTAAC GAGTGCTAAT 
AAGCTGACTA ATAAAGTTGT TCAGACTTTA CGATCCCTGT ATGCCGCCAA GGATGGGACT 
TCCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024837122.1
CDS	500..8668
Translation	MNGDMPHVPITTLAGIASLTDLLNQLPLPSPLPATTTKSLLFNARIAEEVNCLLACRDDNLVSQLVHSLNQVSTDHIELKDNLGSDDPEGDIPVLLQAVLARSPNVFREKSMQNRYVQSGMMMSQYKLSQNSMHSSPASSNYQQTTISHSPSSRFVPPQTSSGNRFMPQQNSPVPSPYAPQSPAGYMPYSHPSSYTTHPQMQQASVSSPIVAGGLRNLHDNKVSGSLSGNSANHHADNPRHGSSDDYLHMVHRISSDDGDSSTMRNAASFPLRSPQPVCSPAGSDGTPKGSRPSLILQSQSLPCSSPRDVPPDILLDSPERKQKKQKKMKLGKDEKDQNEKAAMYDIISSPSKDSTKLTLRLTRVRSSDMDQQEDMLSGMENSNVSENDIPFNVQYPGQTSKTPITPQDVNRPLNAAQCLSQQEQTAFLPANQVPVLQQNTSVATKQPQTSVVLNQQQVSQQGPIYDEVELDALAEIERIERESAIERERFSKEVQDKDKPLKKRKQDSYPQEAGGATGGNRPASQETGSTGNGSRPALMVSIDLHQAGRVDSQASITQDSDSIKKPEEIKQCNDTPISVLQEDIIGNLKSTPENHPETPKKKSDPELLKSEVKQNESRLAESKPNENRLVETKSSETKLETKIEVPTEEPKQNENRMIESRQNESSTAEPKQNENRLPDTKPNDNKQNNGRSETTKSRPETPKQKGESRPETPKQKSDGRPETPKQKGDGRPETPKQKGEGRPETPKQKNEGRPETPKHRHENRRDSGKPSTEKKPEVSKHKQDIKSDSPRLKSERAEALKQRPDGRSISESLRRDHDSKQKSDDRSESERHRGDQSRVRRPETLRSSSRNDHGIKSDGSKPDKLERKHRHESGDSRERPSSGEQKSRPDSPRVKQGDSNKSRPDKPGFKSPNSKDDRRTEGNKSKVDSNKTHPDNKAEFPSYLLGGRSGALKNFVIPKIKRDKDGNITQETKKMEMKGEQKDKVEKMGLVEDLNKGAKPVVVLQKLSLDDVQKLIKDREDKSRSSLKPIKNKPSKSNKGSIDQSVLKELPPELLAEIESTMPLCERVKMNKRKRSTVNEKPKYAEISSDEDNDSDETFESSRKRHKKDDDKAWEYEERDRRSSGDHRRSGHSHEGRRSSGGGRYRNRSPSDSDMEDYSPPPSLSEVARKMKKKEKQKKRKAYEPKLTPEEMMDSSTFKRFTASIENILDNLEDMDFTAFGDDDEIPQELLLGKHQLNELGSESAKIKAMGIMDKLSTDKTVKVLNILEKNIQDGSKLSTLLNHNNDTEEEERLWRDLIMERVTKSADACLTTINIMTSPNMPKAVYIEDVIERVIQYTKFHLQNTLYPQYDPVYRLDPHGGGLLSSKAKRAKCSTHKQRVIVMLYNKVCDIVSSLSELLEIQLLTDTTILQVSSMGITPFFVENVSELQLCAIKLVTAVFSRYEKHRQLILEEIFTSLARLPTSKRSLRNFRLNSSDMDGEPMYIQMVTALVLQLIQCVVHLPSSEKDSNSEEDSNKKVDQDVIITNSYETAMRTAQNFLSIFLKKCGSKQGEEDYRPLFENFVQDLLSTVNKPEWPAAELLLSLLGRLLVHQFSNKSTEMALRVASLDYLGTVAARLRKDAVTSKMDQGSIERILKQVSGGEDEIQQLQKALLDYLDENTETDPSLVFSRKFYIAQWFRDTTLETEKAMKSQKDEESSEGSHHAKEVETTGQIMHRAENRKKFLRSIIKTTPSQFSTLKMNSDTVDYDDACLIVRYLASMRPFAQSFDIYLTQILRVLGENAIAVRTKAMKCLSEVVAVDPSILARLDMQRGVHGRLMDNSTSVREAAVELLGRFVLCRPQLAEQYYDMLIERILDTGISVRKRVIKILRDICIEQPTFPKITEMCVKMIRRVNDEEGIKKLVNETFQKLWFTPTPHNDKEAMTRKILNITDVVAACRDTGYDWFEQLLQNTQNDFMVICNVAKILELVVPLMEHPSETFLATIEEDLMKLIIKYGMTVVQHCVSCLGAVVNKVTQNFKFVWACFNRYYGAISKLKSQHQEDPNNTSLLTNKPALLRSLFTVGALCRHFDFDLEDFKGNSKVNIKDKVLELLMYFTKHSDEEVQTKAIIGLGFAFIQHPSLMFEQEVKNLYNNILSDKNSSVNLKIQVLKNLQTYLQEEDTRMQQADRDWKKVAKQEDLKEMGDVSSGMSSSIMQLYLKQVLEAFFHTQSSVRHFALNVIALTLNQGLIHPVQCVPYLIAMGTDPEPAMRNKADQQLVEIDKKYAGFIHMKAVAGMKMSYQVQQAINTCLKDPVRGFRQDESSSALCSHLYSMIRGNRQHRRAFLISLLNLFDDTAKTEVNMLLYIADNLACFPYQTQEEPLFIMHHIDITLSVSGSNLLQSFKESMVKDKRKERKSSPGKENESSESEEEVSRPRKSRKRVDSDSDSDSEDDINSVMKCLPENSAPLIEFANVSQGILLLLMLKQHLKNLCGFSDSKIQKYSPSESAKVYDKAINRKTGVHFHPKQTLDFLRSDMANSKITEDVKRSIVKQYLDFKLLMEHLDPDEEEEEGEVSASTNARNKAITSLLGGGSPKNNAAAETEDDESDGEDRGGGTSGSLRRSKRNSDSTELAAQMNESVDVMDVIAICCPKYKDRPQIARVVQKTSSGFSVQWMAGSYSGSWTEAKRRDGRKLVPWVDTIKESDIIYKKIALTSANKLTNKVVQTLRSLYAAKDGTSS

Target ORF information:

RefSeq Version	XM_024981354.1
Organism	Bos taurus(cattle)
Definition	Bos taurus NIPBL, cohesin loading factor (NIPBL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024981354.1

ORF Insert Sequence:

ATGAATGGGG ATATGCCCCA TGTCCCCATC ACTACTCTTG CGGGGATTGC TAGTCTTACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCC CCTTTACCTG CTACAACTAC AAAGAGCCTT 
CTCTTTAATG CACGAATAGC AGAAGAAGTG AACTGCCTTT TGGCCTGTAG GGATGATAAT 
TTGGTTTCAC AGCTTGTCCA CAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA 
GATAACCTTG GCAGTGATGA TCCAGAAGGC GACATACCAG TCTTGTTGCA GGCCGTCCTG 
GCGAGGAGTC CTAACGTTTT CAGGGAGAAA AGTATGCAGA ACAGATATGT ACAAAGTGGA 
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC 
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA 
AGCTCTGGGA ACAGATTTAT GCCACAACAA AATAGCCCAG TGCCTAGTCC ATATGCCCCA 
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTATACAAC ACATCCACAG 
ATGCAGCAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CCTACATGAT 
AATAAAGTTT CTGGTTCGTT GTCTGGCAAT TCTGCTAATC ATCATGCTGA TAATCCTAGA 
CATGGCTCAA GTGACGACTA CCTACACATG GTGCACAGGA TAAGTAGTGA CGATGGAGAT 
TCTTCAACAA TGAGGAATGC TGCGTCTTTT CCCTTGAGGT CTCCACAGCC AGTATGTTCC 
CCTGCTGGAA GTGATGGGAC TCCAAAAGGA TCAAGACCAT CTTTAATCCT GCAGTCTCAG 
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGACA TCTTGTTAGA TTCTCCAGAA 
AGAAAGCAAA AGAAGCAAAA GAAAATGAAA TTAGGCAAAG ATGAAAAAGA CCAGAATGAG 
AAGGCTGCAA TGTATGATAT CATTAGCTCT CCATCCAAGG ACTCCACTAA ACTTACGTTA 
AGACTTACTC GTGTAAGATC TTCAGACATG GACCAGCAAG AGGATATGCT TTCTGGTATG 
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT 
TCAAAAACAC CCATTACTCC ACAGGATGTA AACCGCCCAC TAAATGCTGC TCAGTGTTTG 
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC 
ACTTCAGTTG CTACAAAACA GCCCCAGACT TCTGTGGTAC TGAATCAGCA ACAGGTATCA 
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA 
GAGAGAGAAT CAGCTATTGA AAGGGAGCGT TTTTCAAAAG AAGTTCAAGA TAAAGATAAG 
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT 
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG 
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCCTCTAT AACTCAGGAT 
TCAGACTCCA TTAAAAAGCC TGAAGAAATC AAACAATGTA ATGACACACC TATTTCTGTT 
CTTCAGGAAG ATATTATTGG AAATCTTAAA TCTACACCAG AAAACCATCC TGAGACTCCT 
AAAAAAAAGT CTGATCCTGA GCTTTTAAAG AGTGAAGTGA AACAGAATGA AAGTAGATTG 
GCAGAGTCTA AACCAAATGA AAACAGGTTG GTAGAGACAA AATCTAGTGA AACTAAGTTA 
GAAACTAAAA TTGAGGTACC AACAGAAGAA CCTAAACAGA ATGAGAACAG AATGATTGAA 
TCCAGACAAA ATGAGAGCAG CACAGCCGAG CCTAAACAAA ATGAAAATAG ACTGCCGGAC 
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGACCC 
GAAACCCCAA AGCAAAAGGG TGAAAGCCGA CCTGAAACTC CAAAACAAAA GAGTGATGGG 
CGTCCTGAAA CCCCCAAACA AAAGGGTGAT GGACGGCCTG AAACTCCAAA GCAGAAAGGT 
GAGGGCCGAC CTGAAACTCC AAAGCAAAAA AATGAAGGTC GGCCTGAAAC ACCGAAACAT 
AGACATGAAA ATAGGAGGGA TTCTGGAAAG CCATCAACAG AGAAAAAACC TGAAGTGTCT 
AAACATAAAC AGGACATTAA ATCTGATTCA CCTCGGTTAA AATCAGAAAG AGCTGAAGCC 
TTAAAGCAGA GACCTGATGG GCGATCCATC TCTGAGTCAC TAAGACGTGA TCATGACAGT 
AAACAAAAGT CAGATGACAG GAGTGAATCT GAACGACATC GAGGGGATCA ATCTAGGGTT 
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGACC ATGGCATTAA ATCTGATGGT 
TCAAAACCTG ATAAACTAGA AAGAAAACAC AGACATGAAT CAGGGGACTC AAGGGAAAGA 
CCATCTTCTG GGGAACAAAA GTCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT 
AATAAATCAA GACCTGATAA GCCTGGTTTT AAATCTCCAA ATAGTAAGGA TGACAGAAGG 
ACAGAGGGTA ACAAGAGTAA AGTAGACAGT AATAAAACGC ACCCTGATAA TAAGGCAGAA 
TTTCCAAGTT ATTTGCTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCAAAA 
ATCAAGAGGG ATAAAGATGG CAATATTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA 
GAGCAGAAAG ACAAAGTAGA AAAAATGGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG 
CCTGTAGTTG TCCTGCAAAA ACTGTCTTTG GATGATGTTC AAAAACTTAT TAAAGATAGA 
GAGGATAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA 
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAA 
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAATAAAC GCAAGCGTAG CACAGTTAAT 
GAAAAACCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAACTTTT 
GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT 
GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGAAGT 
TCAGGTGGTG GTCGTTATCG AAACCGAAGC CCATCAGATT CTGACATGGA AGATTATTCT 
CCTCCTCCCA GCCTTAGTGA GGTCGCTAGA AAAATGAAGA AAAAAGAAAA ACAGAAGAAG 
CGGAAAGCTT ATGAACCAAA ACTAACACCC GAAGAAATGA TGGATTCTTC AACTTTTAAG 
AGATTCACAG CCTCAATAGA GAATATTTTG GATAACTTAG AAGATATGGA TTTTACTGCA 
TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA 
CTTGGCAGTG AATCTGCTAA AATTAAAGCA ATGGGTATAA TGGATAAGCT TTCCACTGAC 
AAAACTGTGA AAGTCCTAAA TATCTTGGAA AAGAATATTC AAGATGGGTC AAAGCTCTCC 
ACTTTGTTAA ATCATAATAA TGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT 
ATGGAGAGAG TGACAAAATC AGCAGATGCC TGTCTTACAA CTATAAACAT CATGACATCC 
CCTAACATGC CAAAAGCTGT ATATATTGAG GATGTAATCG AAAGAGTTAT ACAGTACACT 
AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT 
CATGGAGGAG GCTTACTAAG TTCAAAGGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG 
AGAGTAATAG TAATGCTTTA TAATAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAACTA 
CTAGAAATAC AACTTCTAAC AGACACAACA ATCCTTCAGG TATCTTCAAT GGGAATAACA 
CCATTTTTTG TGGAAAATGT CAGTGAACTA CAATTGTGTG CCATTAAATT AGTTACTGCA 
GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTG 
GCAAGATTAC CTACCAGCAA GAGGAGCTTA AGGAACTTCA GGTTAAATAG TAGTGATATG 
GATGGAGAGC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT AATTCAGTGT 
GTAGTACACT TACCATCATC AGAGAAGGAC TCTAATTCAG AAGAGGACTC AAATAAAAAA 
GTTGATCAAG ATGTTATCAT TACCAATTCT TATGAAACAG CCATGCGAAC AGCCCAAAAC 
TTCCTTTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA 
CTATTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT 
GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAATAAGTCA 
ACAGAGATGG CTTTAAGAGT GGCATCCCTT GATTACCTTG GAACTGTCGC TGCACGACTG 
AGGAAAGATG CTGTTACAAG CAAAATGGAT CAAGGGTCTA TAGAACGAAT TTTAAAACAG 
GTTTCAGGAG GGGAAGATGA AATACAGCAA TTACAAAAAG CATTGCTTGA TTACTTGGAT 
GAAAACACGG AGACTGATCC TTCACTGGTG TTTTCTCGTA AATTCTATAT TGCCCAGTGG 
TTCCGTGATA CAACACTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA 
TCTGAAGGAT CACATCATGC AAAGGAAGTT GAGACAACTG GTCAAATTAT GCATCGAGCT 
GAAAACCGGA AGAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTCAGCACA 
TTAAAAATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GTTTGATTGT TCGATACTTG 
GCCTCTATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTTCGAGTC 
CTTGGTGAAA ATGCAATTGC CGTTCGAACA AAAGCCATGA AGTGTTTATC TGAGGTTGTT 
GCTGTAGACC CCAGTATTCT AGCAAGGCTG GATATGCAGC GAGGAGTTCA TGGACGATTA 
ATGGATAACT CCACTAGTGT CAGAGAAGCA GCAGTGGAAT TACTAGGTCG ATTTGTCCTT 
TGTCGACCTC AGCTTGCTGA GCAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT 
GGTATCAGTG TCAGAAAAAG GGTGATAAAG ATTCTAAGAG ACATCTGTAT TGAACAACCG 
ACATTTCCAA AAATTACAGA AATGTGTGTA AAAATGATTC GCAGGGTAAA TGATGAAGAG 
GGCATTAAGA AATTAGTAAA TGAAACATTC CAAAAACTCT GGTTTACTCC AACTCCACAC 
AATGACAAAG AAGCAATGAC AAGGAAGATT TTAAATATCA CTGATGTGGT TGCAGCATGC 
AGAGATACTG GATATGACTG GTTTGAGCAG CTTCTTCAAA ATACACAAAA TGATTTCATG 
GTTATCTGCA ATGTTGCAAA AATTCTAGAA CTAGTTGTAC CACTGATGGA ACATCCGAGT 
GAAACTTTCC TTGCCACTAT TGAAGAAGAT CTAATGAAGC TTATCATCAA ATATGGCATG 
ACTGTAGTGC AACATTGTGT AAGCTGTCTT GGGGCTGTTG TAAATAAAGT GACACAAAAT 
TTTAAATTTG TGTGGGCCTG TTTCAATAGA TACTATGGTG CCATTTCAAA ATTAAAAAGT 
CAACACCAAG AGGACCCAAA TAACACTTCA CTGCTAACAA ACAAACCAGC ACTTTTGAGA 
TCCCTATTCA CTGTTGGGGC ACTGTGTCGG CATTTTGATT TTGATCTGGA AGATTTTAAA 
GGCAACAGTA AGGTTAACAT AAAGGATAAA GTACTTGAAC TATTGATGTA TTTTACAAAG 
CATTCAGATG AAGAAGTGCA AACAAAAGCC ATCATTGGTC TAGGATTTGC CTTTATTCAG 
CACCCAAGTC TAATGTTTGA GCAAGAGGTG AAAAATCTAT ACAATAATAT TTTATCTGAT 
AAAAACTCCT CAGTAAATTT AAAAATACAA GTGTTAAAAA ACCTCCAGAC CTACCTACAA 
GAAGAAGATA CACGTATGCA GCAGGCAGAT AGAGACTGGA AGAAGGTTGC AAAACAGGAA 
GATTTGAAAG AAATGGGTGA TGTTTCCTCA GGGATGAGTA GTTCCATCAT GCAGCTTTAC 
CTCAAACAGG TGCTTGAGGC GTTTTTTCAC ACCCAATCAA GTGTACGCCA CTTCGCCCTC 
AATGTCATTG CATTGACTCT AAACCAAGGT CTTATTCATC CAGTTCAGTG TGTGCCGTAT 
TTAATTGCTA TGGGCACAGA CCCAGAACCA GCTATGCGGA ACAAGGCTGA TCAGCAGCTT 
GTGGAAATAG ACAAAAAATA TGCTGGATTC ATTCACATGA AAGCAGTGGC TGGCATGAAG 
ATGTCTTACC AGGTACAACA GGCAATCAAC ACGTGCCTAA AAGATCCTGT AAGGGGTTTC 
CGGCAGGACG AGTCCTCCAG TGCTTTGTGT TCACACCTTT ACTCCATGAT CCGTGGAAAC 
CGCCAGCACA GACGCGCCTT TCTGATTTCT TTACTCAACC TCTTTGATGA CACAGCAAAA 
ACAGAAGTGA ATATGCTCTT GTATATAGCA GACAATCTAG CCTGTTTTCC ATACCAGACA 
CAGGAAGAGC CGTTGTTTAT AATGCATCAT ATAGACATTA CACTCTCTGT TTCTGGTAGT 
AACCTACTGC AGTCATTCAA GGAGTCCATG GTAAAAGACA AAAGGAAAGA GAGAAAATCA 
TCTCCTGGTA AGGAAAATGA GTCAAGCGAG AGTGAAGAAG AAGTCTCTAG GCCTCGAAAG 
TCACGGAAAC GTGTAGATTC AGATTCAGAT TCGGATTCAG AAGATGATAT AAATTCGGTG 
ATGAAATGTT TGCCAGAAAA TTCAGCTCCT TTAATTGAGT TTGCAAATGT CTCCCAGGGT 
ATTTTATTGC TTCTGATGTT AAAGCAACAT TTGAAGAATC TCTGTGGATT TTCTGATAGT 
AAAATTCAGA AATACTCTCC ATCCGAATCT GCAAAAGTGT ATGATAAAGC GATAAACCGG 
AAAACAGGAG TTCATTTTCA TCCCAAGCAA ACACTGGACT TCCTGCGGAG TGATATGGCT 
AATTCCAAAA TCACTGAAGA TGTGAAGAGG AGTATAGTAA AACAGTACCT AGATTTCAAA 
CTTCTCATGG AACATCTGGA CCCTGATGAA GAGGAAGAAG AAGGGGAGGT GTCAGCCAGC 
ACAAATGCTC GGAACAAAGC AATTACCTCG CTGCTTGGAG GAGGCAGCCC TAAAAATAAT 
GCAGCAGCAG AGACAGAAGA TGATGAAAGT GATGGGGAGG ATAGAGGAGG AGGCACTTCA 
GGGTCATTGA GAAGGTCAAA ACGAAATTCA GACTCTACGG AGTTGGCAGC ACAGATGAAT 
GAAAGTGTTG ACGTCATGGA TGTCATCGCT ATTTGCTGTC CAAAGTACAA AGACCGACCA 
CAGATTGCAA GAGTAGTACA GAAAACCAGC AGTGGCTTCA GTGTTCAGTG GATGGCAGGC 
TCCTACAGTG GCTCCTGGAC TGAGGCTAAG CGCCGTGATG GCCGCAAACT GGTGCCTTGG 
GTAGACACTA TTAAAGAGTC AGACATTATT TACAAAAAAA TTGCTCTAAC GAGTGCTAAT 
AAGCTGACTA ATAAAGTTGT TCAGACTTTA CGATCCCTGT ATGCCGCCAA GGATGGGACT 
TCCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIPBL ( XM_005221614.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005221671.1 Bos taurus NIPBL, cohesin loading factor (NIPBL), transcript variant X2, mRNA.

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CloneID	OBa228058
Clone ID Related Accession (Same CDS sequence)	XM_005221614.3
Accession Version	XM_005221614.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	nipped-B-like protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005221614.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGGG ATATGCCCCA TGTCCCCATC ACTACTCTTG CGGGGATTGC TAGTCTTACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCC CCTTTACCTG CTACAACTAC AAAGAGCCTT 
CTCTTTAATG CACGAATAGC AGAAGAAGTG AACTGCCTTT TGGCCTGTAG GGATGATAAT 
TTGGTTTCAC AGCTTGTCCA CAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA 
GATAACCTTG GCAGTGATGA TCCAGAAGGC GACATACCAG TCTTGTTGCA GGCCGTCCTG 
GCGAGGAGTC CTAACGTTTT CAGGGAGAAA AGTATGCAGA ACAGATATGT ACAAAGTGGA 
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC 
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA 
AGCTCTGGGA ACAGATTTAT GCCACAACAA AATAGCCCAG TGCCTAGTCC ATATGCCCCA 
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTATACAAC ACATCCACAG 
ATGCAGCAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CCTACATGAT 
AATAAAGTTT CTGGTTCGTT GTCTGGCAAT TCTGCTAATC ATCATGCTGA TAATCCTAGA 
CATGGCTCAA GTGACGACTA CCTACACATG GTGCACAGGA TAAGTAGTGA CGATGGAGAT 
TCTTCAACAA TGAGGAATGC TGCGTCTTTT CCCTTGAGGT CTCCACAGCC AGTATGTTCC 
CCTGCTGGAA GTGATGGGAC TCCAAAAGGA TCAAGACCAT CTTTAATCCT GCAGTCTCAG 
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGACA TCTTGTTAGA TTCTCCAGAA 
AGAAAGCAAA AGAAGCAAAA GAAAATGAAA TTAGGCAAAG ATGAAAAAGA CCAGAATGAG 
AAGGCTGCAA TGTATGATAT CATTAGCTCT CCATCCAAGG ACTCCACTAA ACTTACGTTA 
AGACTTACTC GTGTAAGATC TTCAGACATG GACCAGCAAG AGGATATGCT TTCTGGTATG 
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT 
TCAAAAACAC CCATTACTCC ACAGGATGTA AACCGCCCAC TAAATGCTGC TCAGTGTTTG 
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC 
ACTTCAGTTG CTACAAAACA GCCCCAGACT TCTGTGGTAC TGAATCAGCA ACAGGTATCA 
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA 
GAGAGAGAAT CAGCTATTGA AAGGGAGCGT TTTTCAAAAG AAGTTCAAGA TAAAGATAAG 
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT 
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG 
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCCTCTAT AACTCAGGAT 
TCAGACTCCA TTAAAAAGCC TGAAGAAATC AAACAATGTA ATGACACACC TATTTCTGTT 
CTTCAGGAAG ATATTATTGG AAATCTTAAA TCTACACCAG AAAACCATCC TGAGACTCCT 
AAAAAAAAGT CTGATCCTGA GCTTTTAAAG AGTGAAGTGA AACAGAATGA AAGTAGATTG 
GCAGAGTCTA AACCAAATGA AAACAGGTTG GTAGAGACAA AATCTAGTGA AACTAAGTTA 
GAAACTAAAA TTGAGGTACC AACAGAAGAA CCTAAACAGA ATGAGAACAG AATGATTGAA 
TCCAGACAAA ATGAGAGCAG CACAGCCGAG CCTAAACAAA ATGAAAATAG ACTGCCGGAC 
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGACCC 
GAAACCCCAA AGCAAAAGGG TGAAAGCCGA CCTGAAACTC CAAAACAAAA GAGTGATGGG 
CGTCCTGAAA CCCCCAAACA AAAGGGTGAT GGACGGCCTG AAACTCCAAA GCAGAAAGGT 
GAGGGCCGAC CTGAAACTCC AAAGCAAAAA AATGAAGGTC GGCCTGAAAC ACCGAAACAT 
AGACATGAAA ATAGGAGGGA TTCTGGAAAG CCATCAACAG AGAAAAAACC TGAAGTGTCT 
AAACATAAAC AGGACATTAA ATCTGATTCA CCTCGGTTAA AATCAGAAAG AGCTGAAGCC 
TTAAAGCAGA GACCTGATGG GCGATCCATC TCTGAGTCAC TAAGACGTGA TCATGACAGT 
AAACAAAAGT CAGATGACAG GAGTGAATCT GAACGACATC GAGGGGATCA ATCTAGGGTT 
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGACC ATGGCATTAA ATCTGATGGT 
TCAAAACCTG ATAAACTAGA AAGAAAACAC AGACATGAAT CAGGGGACTC AAGGGAAAGA 
CCATCTTCTG GGGAACAAAA GTCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT 
AATAAATCAA GACCTGATAA GCCTGGTTTT AAATCTCCAA ATAGTAAGGA TGACAGAAGG 
ACAGAGGGTA ACAAGAGTAA AGTAGACAGT AATAAAACGC ACCCTGATAA TAAGGCAGAA 
TTTCCAAGTT ATTTGCTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCAAAA 
ATCAAGAGGG ATAAAGATGG CAATATTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA 
GAGCAGAAAG ACAAAGTAGA AAAAATGGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG 
CCTGTAGTTG TCCTGCAAAA ACTGTCTTTG GATGATGTTC AAAAACTTAT TAAAGATAGA 
GAGGATAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA 
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAA 
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAATAAAC GCAAGCGTAG CACAGTTAAT 
GAAAAACCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAACTTTT 
GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT 
GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGAAGT 
TCAGGTGGTG GTCGTTATCG AAACCGAAGC CCATCAGATT CTGACATGGA AGATTATTCT 
CCTCCTCCCA GCCTTAGTGA GGTCGCTAGA AAAATGAAGA AAAAAGAAAA ACAGAAGAAG 
CGGAAAGCTT ATGAACCAAA ACTAACACCC GAAGAAATGA TGGATTCTTC AACTTTTAAG 
AGATTCACAG CCTCAATAGA GAATATTTTG GATAACTTAG AAGATATGGA TTTTACTGCA 
TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA 
CTTGGCAGTG AATCTGCTAA AATTAAAGCA ATGGGTATAA TGGATAAGCT TTCCACTGAC 
AAAACTGTGA AAGTCCTAAA TATCTTGGAA AAGAATATTC AAGATGGGTC AAAGCTCTCC 
ACTTTGTTAA ATCATAATAA TGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT 
ATGGAGAGAG TGACAAAATC AGCAGATGCC TGTCTTACAA CTATAAACAT CATGACATCC 
CCTAACATGC CAAAAGCTGT ATATATTGAG GATGTAATCG AAAGAGTTAT ACAGTACACT 
AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT 
CATGGAGGAG GCTTACTAAG TTCAAAGGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG 
AGAGTAATAG TAATGCTTTA TAATAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAACTA 
CTAGAAATAC AACTTCTAAC AGACACAACA ATCCTTCAGG TATCTTCAAT GGGAATAACA 
CCATTTTTTG TGGAAAATGT CAGTGAACTA CAATTGTGTG CCATTAAATT AGTTACTGCA 
GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTG 
GCAAGATTAC CTACCAGCAA GAGGAGCTTA AGGAACTTCA GGTTAAATAG TAGTGATATG 
GATGGAGAGC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT AATTCAGTGT 
GTAGTACACT TACCATCATC AGAGAAGGAC TCTAATTCAG AAGAGGACTC AAATAAAAAA 
GTTGATCAAG ATGTTATCAT TACCAATTCT TATGAAACAG CCATGCGAAC AGCCCAAAAC 
TTCCTTTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA 
CTATTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT 
GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAATAAGTCA 
ACAGAGATGG CTTTAAGAGT GGCATCCCTT GATTACCTTG GAACTGTCGC TGCACGACTG 
AGGAAAGATG CTGTTACAAG CAAAATGGAT CAAGGGTCTA TAGAACGAAT TTTAAAACAG 
GTTTCAGGAG GGGAAGATGA AATACAGCAA TTACAAAAAG CATTGCTTGA TTACTTGGAT 
GAAAACACGG AGACTGATCC TTCACTGGTG TTTTCTCGTA AATTCTATAT TGCCCAGTGG 
TTCCGTGATA CAACACTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA 
TCTGAAGGAT CACATCATGC AAAGGAAGTT GAGACAACTG GTCAAATTAT GCATCGAGCT 
GAAAACCGGA AGAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTCAGCACA 
TTAAAAATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GTTTGATTGT TCGATACTTG 
GCCTCTATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTTCGAGTC 
CTTGGTGAAA ATGCAATTGC CGTTCGAACA AAAGCCATGA AGTGTTTATC TGAGGTTGTT 
GCTGTAGACC CCAGTATTCT AGCAAGGCTG GATATGCAGC GAGGAGTTCA TGGACGATTA 
ATGGATAACT CCACTAGTGT CAGAGAAGCA GCAGTGGAAT TACTAGGTCG ATTTGTCCTT 
TGTCGACCTC AGCTTGCTGA GCAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT 
GGTATCAGTG TCAGAAAAAG GGTGATAAAG ATTCTAAGAG ACATCTGTAT TGAACAACCG 
ACATTTCCAA AAATTACAGA AATGTGTGTA AAAATGATTC GCAGGGTAAA TGATGAAGAG 
GGCATTAAGA AATTAGTAAA TGAAACATTC CAAAAACTCT GGTTTACTCC AACTCCACAC 
AATGACAAAG AAGCAATGAC AAGGAAGATT TTAAATATCA CTGATGTGGT TGCAGCATGC 
AGAGATACTG GATATGACTG GTTTGAGCAG CTTCTTCAAA ATTTGTTGAA GTCTGAAGAG 
GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG 
CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA 
AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG 
GTTAAACATG CCATGACTAT GCAGCCATAT CTTACCACTA AATGTAGTAC ACAAAATGAT 
TTCATGGTTA TCTGCAATGT TGCAAAAATT CTAGAACTAG TTGTACCACT GATGGAACAT 
CCGAGTGAAA CTTTCCTTGC CACTATTGAA GAAGATCTAA TGAAGCTTAT CATCAAATAT 
GGCATGACTG TAGTGCAACA TTGTGTAAGC TGTCTTGGGG CTGTTGTAAA TAAAGTGACA 
CAAAATTTTA AATTTGTGTG GGCCTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA 
AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTGC TAACAAACAA ACCAGCACTT 
TTGAGATCCC TATTCACTGT TGGGGCACTG TGTCGGCATT TTGATTTTGA TCTGGAAGAT 
TTTAAAGGCA ACAGTAAGGT TAACATAAAG GATAAAGTAC TTGAACTATT GATGTATTTT 
ACAAAGCATT CAGATGAAGA AGTGCAAACA AAAGCCATCA TTGGTCTAGG ATTTGCCTTT 
ATTCAGCACC CAAGTCTAAT GTTTGAGCAA GAGGTGAAAA ATCTATACAA TAATATTTTA 
TCTGATAAAA ACTCCTCAGT AAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC 
CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA GGTTGCAAAA 
CAGGAAGATT TGAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG 
CTTTACCTCA AACAGGTGCT TGAGGCGTTT TTTCACACCC AATCAAGTGT ACGCCACTTC 
GCCCTCAATG TCATTGCATT GACTCTAAAC CAAGGTCTTA TTCATCCAGT TCAGTGTGTG 
CCGTATTTAA TTGCTATGGG CACAGACCCA GAACCAGCTA TGCGGAACAA GGCTGATCAG 
CAGCTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ACATGAAAGC AGTGGCTGGC 
ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACGT GCCTAAAAGA TCCTGTAAGG 
GGTTTCCGGC AGGACGAGTC CTCCAGTGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT 
GGAAACCGCC AGCACAGACG CGCCTTTCTG ATTTCTTTAC TCAACCTCTT TGATGACACA 
GCAAAAACAG AAGTGAATAT GCTCTTGTAT ATAGCAGACA ATCTAGCCTG TTTTCCATAC 
CAGACACAGG AAGAGCCGTT GTTTATAATG CATCATATAG ACATTACACT CTCTGTTTCT 
GGTAGTAACC TACTGCAGTC ATTCAAGGAG TCCATGGTAA AAGACAAAAG GAAAGAGAGA 
AAATCATCTC CTGGTAAGGA AAATGAGTCA AGCGAGAGTG AAGAAGAAGT CTCTAGGCCT 
CGAAAGTCAC GGAAACGTGT AGATTCAGAT TCAGATTCGG ATTCAGAAGA TGATATAAAT 
TCGGTGATGA AATGTTTGCC AGAAAATTCA GCTCCTTTAA TTGAGTTTGC AAATGTCTCC 
CAGGGTATTT TATTGCTTCT GATGTTAAAG CAACATTTGA AGAATCTCTG TGGATTTTCT 
GATAGTAAAA TTCAGAAATA CTCTCCATCC GAATCTGCAA AAGTGTATGA TAAAGCGATA 
AACCGGAAAA CAGGAGTTCA TTTTCATCCC AAGCAAACAC TGGACTTCCT GCGGAGTGAT 
ATGGCTAATT CCAAAATCAC TGAAGATGTG AAGAGGAGTA TAGTAAAACA GTACCTAGAT 
TTCAAACTTC TCATGGAACA TCTGGACCCT GATGAAGAGG AAGAAGAAGG GGAGGTGTCA 
GCCAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCGCTGC TTGGAGGAGG CAGCCCTAAA 
AATAATGCAG CAGCAGAGAC AGAAGATGAT GAAAGTGATG GGGAGGATAG AGGAGGAGGC 
ACTTCAGGGG TGAGGCGGAG GAGGAGTCAA CGTATTTCGC AGCGTATTAC TCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005221671.1
CDS	500..8596
Translation	MNGDMPHVPITTLAGIASLTDLLNQLPLPSPLPATTTKSLLFNARIAEEVNCLLACRDDNLVSQLVHSLNQVSTDHIELKDNLGSDDPEGDIPVLLQAVLARSPNVFREKSMQNRYVQSGMMMSQYKLSQNSMHSSPASSNYQQTTISHSPSSRFVPPQTSSGNRFMPQQNSPVPSPYAPQSPAGYMPYSHPSSYTTHPQMQQASVSSPIVAGGLRNLHDNKVSGSLSGNSANHHADNPRHGSSDDYLHMVHRISSDDGDSSTMRNAASFPLRSPQPVCSPAGSDGTPKGSRPSLILQSQSLPCSSPRDVPPDILLDSPERKQKKQKKMKLGKDEKDQNEKAAMYDIISSPSKDSTKLTLRLTRVRSSDMDQQEDMLSGMENSNVSENDIPFNVQYPGQTSKTPITPQDVNRPLNAAQCLSQQEQTAFLPANQVPVLQQNTSVATKQPQTSVVLNQQQVSQQGPIYDEVELDALAEIERIERESAIERERFSKEVQDKDKPLKKRKQDSYPQEAGGATGGNRPASQETGSTGNGSRPALMVSIDLHQAGRVDSQASITQDSDSIKKPEEIKQCNDTPISVLQEDIIGNLKSTPENHPETPKKKSDPELLKSEVKQNESRLAESKPNENRLVETKSSETKLETKIEVPTEEPKQNENRMIESRQNESSTAEPKQNENRLPDTKPNDNKQNNGRSETTKSRPETPKQKGESRPETPKQKSDGRPETPKQKGDGRPETPKQKGEGRPETPKQKNEGRPETPKHRHENRRDSGKPSTEKKPEVSKHKQDIKSDSPRLKSERAEALKQRPDGRSISESLRRDHDSKQKSDDRSESERHRGDQSRVRRPETLRSSSRNDHGIKSDGSKPDKLERKHRHESGDSRERPSSGEQKSRPDSPRVKQGDSNKSRPDKPGFKSPNSKDDRRTEGNKSKVDSNKTHPDNKAEFPSYLLGGRSGALKNFVIPKIKRDKDGNITQETKKMEMKGEQKDKVEKMGLVEDLNKGAKPVVVLQKLSLDDVQKLIKDREDKSRSSLKPIKNKPSKSNKGSIDQSVLKELPPELLAEIESTMPLCERVKMNKRKRSTVNEKPKYAEISSDEDNDSDETFESSRKRHKKDDDKAWEYEERDRRSSGDHRRSGHSHEGRRSSGGGRYRNRSPSDSDMEDYSPPPSLSEVARKMKKKEKQKKRKAYEPKLTPEEMMDSSTFKRFTASIENILDNLEDMDFTAFGDDDEIPQELLLGKHQLNELGSESAKIKAMGIMDKLSTDKTVKVLNILEKNIQDGSKLSTLLNHNNDTEEEERLWRDLIMERVTKSADACLTTINIMTSPNMPKAVYIEDVIERVIQYTKFHLQNTLYPQYDPVYRLDPHGGGLLSSKAKRAKCSTHKQRVIVMLYNKVCDIVSSLSELLEIQLLTDTTILQVSSMGITPFFVENVSELQLCAIKLVTAVFSRYEKHRQLILEEIFTSLARLPTSKRSLRNFRLNSSDMDGEPMYIQMVTALVLQLIQCVVHLPSSEKDSNSEEDSNKKVDQDVIITNSYETAMRTAQNFLSIFLKKCGSKQGEEDYRPLFENFVQDLLSTVNKPEWPAAELLLSLLGRLLVHQFSNKSTEMALRVASLDYLGTVAARLRKDAVTSKMDQGSIERILKQVSGGEDEIQQLQKALLDYLDENTETDPSLVFSRKFYIAQWFRDTTLETEKAMKSQKDEESSEGSHHAKEVETTGQIMHRAENRKKFLRSIIKTTPSQFSTLKMNSDTVDYDDACLIVRYLASMRPFAQSFDIYLTQILRVLGENAIAVRTKAMKCLSEVVAVDPSILARLDMQRGVHGRLMDNSTSVREAAVELLGRFVLCRPQLAEQYYDMLIERILDTGISVRKRVIKILRDICIEQPTFPKITEMCVKMIRRVNDEEGIKKLVNETFQKLWFTPTPHNDKEAMTRKILNITDVVAACRDTGYDWFEQLLQNLLKSEEDSSYKPVKKACTQLVDNLVEHILKYEESLADSDNKGVNSGRLVACITTLFLFSKIRPQLMVKHAMTMQPYLTTKCSTQNDFMVICNVAKILELVVPLMEHPSETFLATIEEDLMKLIIKYGMTVVQHCVSCLGAVVNKVTQNFKFVWACFNRYYGAISKLKSQHQEDPNNTSLLTNKPALLRSLFTVGALCRHFDFDLEDFKGNSKVNIKDKVLELLMYFTKHSDEEVQTKAIIGLGFAFIQHPSLMFEQEVKNLYNNILSDKNSSVNLKIQVLKNLQTYLQEEDTRMQQADRDWKKVAKQEDLKEMGDVSSGMSSSIMQLYLKQVLEAFFHTQSSVRHFALNVIALTLNQGLIHPVQCVPYLIAMGTDPEPAMRNKADQQLVEIDKKYAGFIHMKAVAGMKMSYQVQQAINTCLKDPVRGFRQDESSSALCSHLYSMIRGNRQHRRAFLISLLNLFDDTAKTEVNMLLYIADNLACFPYQTQEEPLFIMHHIDITLSVSGSNLLQSFKESMVKDKRKERKSSPGKENESSESEEEVSRPRKSRKRVDSDSDSDSEDDINSVMKCLPENSAPLIEFANVSQGILLLLMLKQHLKNLCGFSDSKIQKYSPSESAKVYDKAINRKTGVHFHPKQTLDFLRSDMANSKITEDVKRSIVKQYLDFKLLMEHLDPDEEEEEGEVSASTNARNKAITSLLGGGSPKNNAAAETEDDESDGEDRGGGTSGVRRRRSQRISQRITH

Target ORF information:

RefSeq Version	XM_005221614.3
Organism	Bos taurus(cattle)
Definition	Bos taurus NIPBL, cohesin loading factor (NIPBL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005221614.3

ORF Insert Sequence:

ATGAATGGGG ATATGCCCCA TGTCCCCATC ACTACTCTTG CGGGGATTGC TAGTCTTACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCC CCTTTACCTG CTACAACTAC AAAGAGCCTT 
CTCTTTAATG CACGAATAGC AGAAGAAGTG AACTGCCTTT TGGCCTGTAG GGATGATAAT 
TTGGTTTCAC AGCTTGTCCA CAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA 
GATAACCTTG GCAGTGATGA TCCAGAAGGC GACATACCAG TCTTGTTGCA GGCCGTCCTG 
GCGAGGAGTC CTAACGTTTT CAGGGAGAAA AGTATGCAGA ACAGATATGT ACAAAGTGGA 
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC 
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA 
AGCTCTGGGA ACAGATTTAT GCCACAACAA AATAGCCCAG TGCCTAGTCC ATATGCCCCA 
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTATACAAC ACATCCACAG 
ATGCAGCAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CCTACATGAT 
AATAAAGTTT CTGGTTCGTT GTCTGGCAAT TCTGCTAATC ATCATGCTGA TAATCCTAGA 
CATGGCTCAA GTGACGACTA CCTACACATG GTGCACAGGA TAAGTAGTGA CGATGGAGAT 
TCTTCAACAA TGAGGAATGC TGCGTCTTTT CCCTTGAGGT CTCCACAGCC AGTATGTTCC 
CCTGCTGGAA GTGATGGGAC TCCAAAAGGA TCAAGACCAT CTTTAATCCT GCAGTCTCAG 
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGACA TCTTGTTAGA TTCTCCAGAA 
AGAAAGCAAA AGAAGCAAAA GAAAATGAAA TTAGGCAAAG ATGAAAAAGA CCAGAATGAG 
AAGGCTGCAA TGTATGATAT CATTAGCTCT CCATCCAAGG ACTCCACTAA ACTTACGTTA 
AGACTTACTC GTGTAAGATC TTCAGACATG GACCAGCAAG AGGATATGCT TTCTGGTATG 
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT 
TCAAAAACAC CCATTACTCC ACAGGATGTA AACCGCCCAC TAAATGCTGC TCAGTGTTTG 
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC 
ACTTCAGTTG CTACAAAACA GCCCCAGACT TCTGTGGTAC TGAATCAGCA ACAGGTATCA 
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA 
GAGAGAGAAT CAGCTATTGA AAGGGAGCGT TTTTCAAAAG AAGTTCAAGA TAAAGATAAG 
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT 
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG 
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCCTCTAT AACTCAGGAT 
TCAGACTCCA TTAAAAAGCC TGAAGAAATC AAACAATGTA ATGACACACC TATTTCTGTT 
CTTCAGGAAG ATATTATTGG AAATCTTAAA TCTACACCAG AAAACCATCC TGAGACTCCT 
AAAAAAAAGT CTGATCCTGA GCTTTTAAAG AGTGAAGTGA AACAGAATGA AAGTAGATTG 
GCAGAGTCTA AACCAAATGA AAACAGGTTG GTAGAGACAA AATCTAGTGA AACTAAGTTA 
GAAACTAAAA TTGAGGTACC AACAGAAGAA CCTAAACAGA ATGAGAACAG AATGATTGAA 
TCCAGACAAA ATGAGAGCAG CACAGCCGAG CCTAAACAAA ATGAAAATAG ACTGCCGGAC 
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGACCC 
GAAACCCCAA AGCAAAAGGG TGAAAGCCGA CCTGAAACTC CAAAACAAAA GAGTGATGGG 
CGTCCTGAAA CCCCCAAACA AAAGGGTGAT GGACGGCCTG AAACTCCAAA GCAGAAAGGT 
GAGGGCCGAC CTGAAACTCC AAAGCAAAAA AATGAAGGTC GGCCTGAAAC ACCGAAACAT 
AGACATGAAA ATAGGAGGGA TTCTGGAAAG CCATCAACAG AGAAAAAACC TGAAGTGTCT 
AAACATAAAC AGGACATTAA ATCTGATTCA CCTCGGTTAA AATCAGAAAG AGCTGAAGCC 
TTAAAGCAGA GACCTGATGG GCGATCCATC TCTGAGTCAC TAAGACGTGA TCATGACAGT 
AAACAAAAGT CAGATGACAG GAGTGAATCT GAACGACATC GAGGGGATCA ATCTAGGGTT 
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGACC ATGGCATTAA ATCTGATGGT 
TCAAAACCTG ATAAACTAGA AAGAAAACAC AGACATGAAT CAGGGGACTC AAGGGAAAGA 
CCATCTTCTG GGGAACAAAA GTCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT 
AATAAATCAA GACCTGATAA GCCTGGTTTT AAATCTCCAA ATAGTAAGGA TGACAGAAGG 
ACAGAGGGTA ACAAGAGTAA AGTAGACAGT AATAAAACGC ACCCTGATAA TAAGGCAGAA 
TTTCCAAGTT ATTTGCTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCAAAA 
ATCAAGAGGG ATAAAGATGG CAATATTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA 
GAGCAGAAAG ACAAAGTAGA AAAAATGGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG 
CCTGTAGTTG TCCTGCAAAA ACTGTCTTTG GATGATGTTC AAAAACTTAT TAAAGATAGA 
GAGGATAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA 
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAA 
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAATAAAC GCAAGCGTAG CACAGTTAAT 
GAAAAACCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAACTTTT 
GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT 
GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGAAGT 
TCAGGTGGTG GTCGTTATCG AAACCGAAGC CCATCAGATT CTGACATGGA AGATTATTCT 
CCTCCTCCCA GCCTTAGTGA GGTCGCTAGA AAAATGAAGA AAAAAGAAAA ACAGAAGAAG 
CGGAAAGCTT ATGAACCAAA ACTAACACCC GAAGAAATGA TGGATTCTTC AACTTTTAAG 
AGATTCACAG CCTCAATAGA GAATATTTTG GATAACTTAG AAGATATGGA TTTTACTGCA 
TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA 
CTTGGCAGTG AATCTGCTAA AATTAAAGCA ATGGGTATAA TGGATAAGCT TTCCACTGAC 
AAAACTGTGA AAGTCCTAAA TATCTTGGAA AAGAATATTC AAGATGGGTC AAAGCTCTCC 
ACTTTGTTAA ATCATAATAA TGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT 
ATGGAGAGAG TGACAAAATC AGCAGATGCC TGTCTTACAA CTATAAACAT CATGACATCC 
CCTAACATGC CAAAAGCTGT ATATATTGAG GATGTAATCG AAAGAGTTAT ACAGTACACT 
AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT 
CATGGAGGAG GCTTACTAAG TTCAAAGGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG 
AGAGTAATAG TAATGCTTTA TAATAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAACTA 
CTAGAAATAC AACTTCTAAC AGACACAACA ATCCTTCAGG TATCTTCAAT GGGAATAACA 
CCATTTTTTG TGGAAAATGT CAGTGAACTA CAATTGTGTG CCATTAAATT AGTTACTGCA 
GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTG 
GCAAGATTAC CTACCAGCAA GAGGAGCTTA AGGAACTTCA GGTTAAATAG TAGTGATATG 
GATGGAGAGC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT AATTCAGTGT 
GTAGTACACT TACCATCATC AGAGAAGGAC TCTAATTCAG AAGAGGACTC AAATAAAAAA 
GTTGATCAAG ATGTTATCAT TACCAATTCT TATGAAACAG CCATGCGAAC AGCCCAAAAC 
TTCCTTTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA 
CTATTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT 
GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAATAAGTCA 
ACAGAGATGG CTTTAAGAGT GGCATCCCTT GATTACCTTG GAACTGTCGC TGCACGACTG 
AGGAAAGATG CTGTTACAAG CAAAATGGAT CAAGGGTCTA TAGAACGAAT TTTAAAACAG 
GTTTCAGGAG GGGAAGATGA AATACAGCAA TTACAAAAAG CATTGCTTGA TTACTTGGAT 
GAAAACACGG AGACTGATCC TTCACTGGTG TTTTCTCGTA AATTCTATAT TGCCCAGTGG 
TTCCGTGATA CAACACTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA 
TCTGAAGGAT CACATCATGC AAAGGAAGTT GAGACAACTG GTCAAATTAT GCATCGAGCT 
GAAAACCGGA AGAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTCAGCACA 
TTAAAAATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GTTTGATTGT TCGATACTTG 
GCCTCTATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTTCGAGTC 
CTTGGTGAAA ATGCAATTGC CGTTCGAACA AAAGCCATGA AGTGTTTATC TGAGGTTGTT 
GCTGTAGACC CCAGTATTCT AGCAAGGCTG GATATGCAGC GAGGAGTTCA TGGACGATTA 
ATGGATAACT CCACTAGTGT CAGAGAAGCA GCAGTGGAAT TACTAGGTCG ATTTGTCCTT 
TGTCGACCTC AGCTTGCTGA GCAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT 
GGTATCAGTG TCAGAAAAAG GGTGATAAAG ATTCTAAGAG ACATCTGTAT TGAACAACCG 
ACATTTCCAA AAATTACAGA AATGTGTGTA AAAATGATTC GCAGGGTAAA TGATGAAGAG 
GGCATTAAGA AATTAGTAAA TGAAACATTC CAAAAACTCT GGTTTACTCC AACTCCACAC 
AATGACAAAG AAGCAATGAC AAGGAAGATT TTAAATATCA CTGATGTGGT TGCAGCATGC 
AGAGATACTG GATATGACTG GTTTGAGCAG CTTCTTCAAA ATTTGTTGAA GTCTGAAGAG 
GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG 
CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA 
AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG 
GTTAAACATG CCATGACTAT GCAGCCATAT CTTACCACTA AATGTAGTAC ACAAAATGAT 
TTCATGGTTA TCTGCAATGT TGCAAAAATT CTAGAACTAG TTGTACCACT GATGGAACAT 
CCGAGTGAAA CTTTCCTTGC CACTATTGAA GAAGATCTAA TGAAGCTTAT CATCAAATAT 
GGCATGACTG TAGTGCAACA TTGTGTAAGC TGTCTTGGGG CTGTTGTAAA TAAAGTGACA 
CAAAATTTTA AATTTGTGTG GGCCTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA 
AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTGC TAACAAACAA ACCAGCACTT 
TTGAGATCCC TATTCACTGT TGGGGCACTG TGTCGGCATT TTGATTTTGA TCTGGAAGAT 
TTTAAAGGCA ACAGTAAGGT TAACATAAAG GATAAAGTAC TTGAACTATT GATGTATTTT 
ACAAAGCATT CAGATGAAGA AGTGCAAACA AAAGCCATCA TTGGTCTAGG ATTTGCCTTT 
ATTCAGCACC CAAGTCTAAT GTTTGAGCAA GAGGTGAAAA ATCTATACAA TAATATTTTA 
TCTGATAAAA ACTCCTCAGT AAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC 
CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA GGTTGCAAAA 
CAGGAAGATT TGAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG 
CTTTACCTCA AACAGGTGCT TGAGGCGTTT TTTCACACCC AATCAAGTGT ACGCCACTTC 
GCCCTCAATG TCATTGCATT GACTCTAAAC CAAGGTCTTA TTCATCCAGT TCAGTGTGTG 
CCGTATTTAA TTGCTATGGG CACAGACCCA GAACCAGCTA TGCGGAACAA GGCTGATCAG 
CAGCTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ACATGAAAGC AGTGGCTGGC 
ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACGT GCCTAAAAGA TCCTGTAAGG 
GGTTTCCGGC AGGACGAGTC CTCCAGTGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT 
GGAAACCGCC AGCACAGACG CGCCTTTCTG ATTTCTTTAC TCAACCTCTT TGATGACACA 
GCAAAAACAG AAGTGAATAT GCTCTTGTAT ATAGCAGACA ATCTAGCCTG TTTTCCATAC 
CAGACACAGG AAGAGCCGTT GTTTATAATG CATCATATAG ACATTACACT CTCTGTTTCT 
GGTAGTAACC TACTGCAGTC ATTCAAGGAG TCCATGGTAA AAGACAAAAG GAAAGAGAGA 
AAATCATCTC CTGGTAAGGA AAATGAGTCA AGCGAGAGTG AAGAAGAAGT CTCTAGGCCT 
CGAAAGTCAC GGAAACGTGT AGATTCAGAT TCAGATTCGG ATTCAGAAGA TGATATAAAT 
TCGGTGATGA AATGTTTGCC AGAAAATTCA GCTCCTTTAA TTGAGTTTGC AAATGTCTCC 
CAGGGTATTT TATTGCTTCT GATGTTAAAG CAACATTTGA AGAATCTCTG TGGATTTTCT 
GATAGTAAAA TTCAGAAATA CTCTCCATCC GAATCTGCAA AAGTGTATGA TAAAGCGATA 
AACCGGAAAA CAGGAGTTCA TTTTCATCCC AAGCAAACAC TGGACTTCCT GCGGAGTGAT 
ATGGCTAATT CCAAAATCAC TGAAGATGTG AAGAGGAGTA TAGTAAAACA GTACCTAGAT 
TTCAAACTTC TCATGGAACA TCTGGACCCT GATGAAGAGG AAGAAGAAGG GGAGGTGTCA 
GCCAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCGCTGC TTGGAGGAGG CAGCCCTAAA 
AATAATGCAG CAGCAGAGAC AGAAGATGAT GAAAGTGATG GGGAGGATAG AGGAGGAGGC 
ACTTCAGGGG TGAGGCGGAG GAGGAGTCAA CGTATTTCGC AGCGTATTAC TCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIPBL ( XM_005221614.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005221671.1 Bos taurus Nipped-B homolog (Drosophila) (NIPBL), transcript variant X1, mRNA.

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CloneID	OBa30682
Clone ID Related Accession (Same CDS sequence)	XM_005221614.2
Accession Version	XM_005221614.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	nipped-B-like protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104515.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005221614.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGGG ATATGCCCCA TGTCCCCATC ACTACTCTTG CGGGGATTGC TAGTCTTACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCC CCTTTACCTG CTACAACTAC AAAGAGCCTT 
CTCTTTAATG CACGAATAGC AGAAGAAGTG AACTGCCTTT TGGCCTGTAG GGATGATAAT 
TTGGTTTCAC AGCTTGTCCA CAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA 
GATAACCTTG GCAGTGATGA TCCAGAAGGC GACATACCAG TCTTGTTGCA GGCCGTCCTG 
GCGAGGAGTC CTAACGTTTT CAGGGAGAAA AGTATGCAGA ACAGATATGT ACAAAGTGGA 
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC 
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA 
AGCTCTGGGA ACAGATTTAT GCCACAACAA AATAGCCCAG TGCCTAGTCC ATATGCCCCA 
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTATACAAC ACATCCACAG 
ATGCAGCAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CCTACATGAT 
AATAAAGTTT CTGGTTCGTT GTCTGGCAAT TCTGCTAATC ATCATGCTGA TAATCCTAGA 
CATGGCTCAA GTGACGACTA CCTACACATG GTGCACAGGA TAAGTAGTGA CGATGGAGAT 
TCTTCAACAA TGAGGAATGC TGCGTCTTTT CCCTTGAGGT CTCCACAGCC AGTATGTTCC 
CCTGCTGGAA GTGATGGGAC TCCAAAAGGA TCAAGACCAT CTTTAATCCT GCAGTCTCAG 
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGACA TCTTGTTAGA TTCTCCAGAA 
AGAAAGCAAA AGAAGCAAAA GAAAATGAAA TTAGGCAAAG ATGAAAAAGA CCAGAATGAG 
AAGGCTGCAA TGTATGATAT CATTAGCTCT CCATCCAAGG ACTCCACTAA ACTTACGTTA 
AGACTTACTC GTGTAAGATC TTCAGACATG GACCAGCAAG AGGATATGCT TTCTGGTATG 
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT 
TCAAAAACAC CCATTACTCC ACAGGATGTA AACCGCCCAC TAAATGCTGC TCAGTGTTTG 
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC 
ACTTCAGTTG CTACAAAACA GCCCCAGACT TCTGTGGTAC TGAATCAGCA ACAGGTATCA 
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA 
GAGAGAGAAT CAGCTATTGA AAGGGAGCGT TTTTCAAAAG AAGTTCAAGA TAAAGATAAG 
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT 
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG 
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCCTCTAT AACTCAGGAT 
TCAGACTCCA TTAAAAAGCC TGAAGAAATC AAACAATGTA ACGACACACC TATTTCTGTT 
CTTCAGGAAG ATATTATTGG AAATCTTAAA TCTACACCAG AAAACCATCC TGAGACTCCT 
AAAAAAAAGT CTGATCCTGA GCTTTTAAAG AGTGAAGTGA AACAGAATGA AAGTAGATTG 
GCAGAGTCTA AACCAAATGA AAACAGATTG GTAGAGACAA AATCTAGTGA AACTAAGTTA 
GAAACTAAAA TTGAGGTACC AACAGAAGAA CCTAAACAGA ATGAGAACAG AATGATTGAA 
TCCAGACAAA ATGAGAGCAG CACAGCCGAG CCTAAACAAA ATGAAAATAG ACTGCCGGAC 
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGACCC 
GAAACCCCAA AGCAAAAGGG TGAAAGCCGA CCTGAAACTC CAAAACAAAA GAGTGATGGG 
CGTCCTGAAA CCCCCAAACA AAAGGGTGAT GGACGGCCTG AAACTCCAAA GCAGAAAGGT 
GAGGGCCGAC CTGAAACTCC AAAGCAAAAA AATGAAGGTC GGCCTGAAAC ACCGAAACAT 
AGACATGAAA ATAGGAGGGA TTCTGGAAAG CCATCAACAG AGAAAAAACC TGAAGTGTCT 
AAACATAAAC AGGACATTAA ATCTGATTCA CCTCGGTTAA AATCAGAAAG AGCTGAAGCC 
TTAAAGCAGA GACCTGATGG GCGATCCATC TCTGAGTCAC TAAGACGTGA TCATGACAGT 
AAACAAAAGT CAGATGACAG GAGTGAATCT GAACGACATC GAGGGGATCA ATCTAGGGTT 
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGACC ATGGCATTAA ATCTGATGGT 
TCAAAACCTG ATAAACTAGA AAGAAAACAC AGACATGAAT CAGGGGACTC AAGGGAAAGA 
CCATCTTCTG GGGAACAAAA GTCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT 
AATAAATCAA GACCTGATAA GCCTGGTTTT AAATCTCCAA ATAGTAAGGA TGACAGAAGG 
ACAGAGGGTA ACAAGAGTAA AGTAGACAGT AATAAAACGC ACCCTGATAA TAAGGCAGAA 
TTTCCAAGTT ATTTGCTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCAAAA 
ATCAAGAGGG ATAAAGATGG CAATATTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA 
GAGCAGAAAG ACAAAGTAGA AAAAATGGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG 
CCTGTAGTTG TCCTGCAAAA ACTGTCTTTG GATGATGTTC AAAAACTTAT TAAAGATAGA 
GAGGATAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA 
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAA 
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAATAAAC GCAAGCGTAG CACAGTTAAT 
GAAAAACCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAACTTTT 
GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT 
GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGAAGT 
TCAGGTGGTG GTCGTTATCG AAACCGAAGC CCATCAGATT CTGACATGGA AGATTATTCT 
CCTCCTCCCA GCCTTAGTGA GGTCGCTAGA AAAATGAAGA AAAAAGAAAA ACAGAAGAAG 
CGGAAAGCTT ATGAACCAAA ACTAACACCC GAAGAAATGA TGGATTCTTC AACTTTTAAG 
AGATTCACAG CCTCAATAGA GAATATTTTG GATAACTTAG AAGATATGGA TTTTACTGCA 
TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA 
CTTGGCAGTG AATCTGCTAA AATTAAAGCA ATGGGTATAA TGGATAAGCT TTCCACTGAC 
AAAACTGTGA AAGTCCTAAA TATCTTGGAA AAGAATATTC AAGATGGGTC AAAGCTCTCC 
ACTTTGTTAA ATCATAATAA TGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT 
ATGGAGAGAG TGACAAAATC AGCAGATGCC TGTCTTACAA CTATAAACAT CATGACATCC 
CCTAACATGC CAAAAGCTGT ATATATTGAG GATGTAATCG AAAGAGTTAT ACAGTACACT 
AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT 
CATGGAGGAG GCTTACTAAG TTCAAAGGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG 
AGAGTAATAG TAATGCTTTA TAATAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAACTA 
CTAGAAATAC AACTTCTAAC AGACACAACA ATCCTTCAGG TATCTTCAAT GGGAATAACA 
CCATTTTTTG TGGAAAATGT CAGTGAACTA CAATTGTGTG CCATTAAATT AGTTACTGCA 
GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTG 
GCAAGATTAC CTACCAGCAA GAGGAGCTTA AGGAACTTCA GGTTAAATAG TAGTGATATG 
GATGGAGAGC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT AATTCAGTGT 
GTAGTACACT TACCATCATC AGAGAAGGAC TCTAATTCAG AAGAGGACTC AAATAAAAAA 
GTTGATCAAG ATGTTATCAT TACCAATTCT TATGAAACAG CCATGCGAAC AGCCCAAAAC 
TTCCTTTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA 
CTATTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT 
GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAATAAGTCA 
ACAGAGATGG CTTTAAGAGT GGCATCCCTT GATTACCTTG GAACTGTCGC TGCACGACTG 
AGGAAAGATG CTGTTACAAG CAAAATGGAT CAAGGGTCTA TAGAACGAAT TTTAAAACAG 
GTTTCAGGAG GGGAAGATGA AATACAGCAA TTACAAAAAG CATTGCTTGA TTACTTGGAT 
GAAAACACGG AGACTGATCC TTCACTGGTG TTTTCTCGTA AATTCTATAT TGCCCAGTGG 
TTCCGTGATA CAACACTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA 
TCTGAAGGAT CACATCATGC AAAGGAAGTT GAGACAACTG GTCAAATTAT GCATCGAGCT 
GAAAACCGGA AGAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTCAGCACA 
TTAAAAATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GTTTGATTGT TCGATACTTG 
GCCTCTATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTTCGAGTC 
CTTGGTGAAA ATGCAATTGC CGTTCGAACA AAAGCCATGA AGTGTTTATC TGAGGTTGTT 
GCTGTAGACC CCAGTATTCT AGCAAGGCTG GATATGCAGC GAGGAGTTCA TGGACGATTA 
ATGGATAACT CCACTAGTGT CAGAGAAGCA GCAGTGGAAT TACTAGGTCG ATTTGTCCTT 
TGTCGACCTC AGCTTGCTGA GCAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT 
GGTATCAGTG TCAGAAAAAG GGTGATAAAG ATTCTAAGAG ACATCTGTAT TGAACAACCG 
ACATTTCCAA AAATTACAGA AATGTGTGTA AAAATGATTC GCAGGGTAAA TGATGAAGAG 
GGCATTAAGA AATTAGTAAA TGAAACATTC CAAAAACTCT GGTTTACTCC AACTCCACAC 
AATGACAAAG AAGCAATGAC AAGGAAGATT TTAAATATCA CTGATGTGGT TGCAGCATGC 
AGAGATACTG GATATGACTG GTTTGAGCAG CTTCTTCAAA ATTTGTTGAA GTCTGAAGAG 
GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG 
CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA 
AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG 
GTTAAACATG CCATGACTAT GCAGCCATAT CTTACCACTA AATGTAGTAC ACAAAATGAT 
TTCATGGTTA TCTGCAATGT TGCAAAAATT CTAGAACTAG TTGTACCACT GATGGAACAT 
CCGAGTGAAA CTTTCCTTGC CACTATTGAA GAAGATCTAA TGAAGCTTAT CATCAAATAT 
GGCATGACTG TAGTGCAACA TTGTGTAAGC TGTCTTGGGG CTGTTGTAAA TAAAGTGACA 
CAAAATTTTA AATTTGTGTG GGCCTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA 
AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTGC TAACAAACAA ACCAGCACTT 
TTGAGATCCC TATTCACTGT TGGGGCACTG TGTCGGCATT TTGATTTTGA TCTGGAAGAT 
TTTAAAGGCA ACAGTAAGGT TAACATAAAG GATAAAGTAC TTGAACTATT GATGTATTTT 
ACAAAGCATT CAGATGAAGA AGTGCAAACA AAAGCCATCA TTGGTCTAGG ATTTGCCTTT 
ATTCAGCACC CAAGTCTAAT GTTTGAGCAA GAGGTGAAAA ATCTATACAA TAATATTTTA 
TCTGATAAAA ACTCCTCAGT AAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC 
CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA GGTTGCAAAA 
CAGGAAGATT TGAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG 
CTTTACCTCA AACAGGTGCT TGAGGCGTTT TTTCACACCC AATCAAGTGT ACGCCACTTC 
GCCCTCAATG TCATTGCATT GACTCTAAAC CAAGGTCTTA TTCATCCAGT TCAGTGTGTG 
CCGTATTTAA TTGCTATGGG CACAGACCCA GAACCAGCTA TGCGGAACAA GGCTGATCAG 
CAGCTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ACATGAAAGC AGTGGCTGGC 
ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACGT GCCTAAAAGA TCCTGTAAGG 
GGTTTCCGGC AGGACGAGTC CTCCAGTGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT 
GGAAACCGCC AGCACAGACG CGCCTTTCTG ATTTCTTTAC TCAACCTCTT TGATGACACA 
GCAAAAACAG AAGTGAATAT GCTCTTGTAT ATAGCAGACA ATCTAGCCTG TTTTCCATAC 
CAGACACAGG AAGAGCCGTT GTTTATAATG CATCATATAG ACATTACACT CTCTGTTTCT 
GGTAGTAACC TACTGCAGTC ATTCAAGGAG TCCATGGTAA AAGACAAAAG GAAAGAGAGA 
AAATCATCTC CTGGTAAGGA AAATGAGTCA AGCGAGAGTG AAGAAGAAGT CTCTAGGCCT 
CGAAAGTCAC GGAAACGTGT AGATTCAGAT TCAGATTCGG ATTCAGAAGA TGATATAAAT 
TCGGTGATGA AATGTTTGCC AGAAAATTCA GCTCCTTTAA TTGAGTTTGC AAATGTCTCC 
CAGGGTATTT TATTGCTTCT GATGTTAAAG CAACATTTGA AGAATCTCTG TGGATTTTCT 
GATAGTAAAA TTCAGAAATA CTCTCCATCC GAATCTGCAA AAGTGTATGA TAAAGCGATA 
AACCGGAAAA CAGGAGTTCA TTTTCATCCC AAGCAAACAC TGGACTTCCT GCGGAGTGAT 
ATGGCTAATT CCAAAATCAC TGAAGATGTG AAGAGGAGTA TAGTAAAACA GTACCTAGAT 
TTCAAACTTC TCATGGAACA TCTGGACCCT GATGAAGAGG AAGAAGAAGG GGAGGTGTCA 
GCCAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCGCTGC TTGGAGGAGG CAGCCCTAAA 
AATAATGCAG CAGCAGAGAC AGAAGATGAT GAAAGTGATG GGGAGGATAG AGGAGGAGGC 
ACTTCAGGGG TGAGGCGGAG GAGGAGTCAA CGTATTTCGC AGCGTATTAC TCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005221671.1
CDS	423..8519
Translation	MNGDMPHVPITTLAGIASLTDLLNQLPLPSPLPATTTKSLLFNARIAEEVNCLLACRDDNLVSQLVHSLNQVSTDHIELKDNLGSDDPEGDIPVLLQAVLARSPNVFREKSMQNRYVQSGMMMSQYKLSQNSMHSSPASSNYQQTTISHSPSSRFVPPQTSSGNRFMPQQNSPVPSPYAPQSPAGYMPYSHPSSYTTHPQMQQASVSSPIVAGGLRNLHDNKVSGSLSGNSANHHADNPRHGSSDDYLHMVHRISSDDGDSSTMRNAASFPLRSPQPVCSPAGSDGTPKGSRPSLILQSQSLPCSSPRDVPPDILLDSPERKQKKQKKMKLGKDEKDQNEKAAMYDIISSPSKDSTKLTLRLTRVRSSDMDQQEDMLSGMENSNVSENDIPFNVQYPGQTSKTPITPQDVNRPLNAAQCLSQQEQTAFLPANQVPVLQQNTSVATKQPQTSVVLNQQQVSQQGPIYDEVELDALAEIERIERESAIERERFSKEVQDKDKPLKKRKQDSYPQEAGGATGGNRPASQETGSTGNGSRPALMVSIDLHQAGRVDSQASITQDSDSIKKPEEIKQCNDTPISVLQEDIIGNLKSTPENHPETPKKKSDPELLKSEVKQNESRLAESKPNENRLVETKSSETKLETKIEVPTEEPKQNENRMIESRQNESSTAEPKQNENRLPDTKPNDNKQNNGRSETTKSRPETPKQKGESRPETPKQKSDGRPETPKQKGDGRPETPKQKGEGRPETPKQKNEGRPETPKHRHENRRDSGKPSTEKKPEVSKHKQDIKSDSPRLKSERAEALKQRPDGRSISESLRRDHDSKQKSDDRSESERHRGDQSRVRRPETLRSSSRNDHGIKSDGSKPDKLERKHRHESGDSRERPSSGEQKSRPDSPRVKQGDSNKSRPDKPGFKSPNSKDDRRTEGNKSKVDSNKTHPDNKAEFPSYLLGGRSGALKNFVIPKIKRDKDGNITQETKKMEMKGEQKDKVEKMGLVEDLNKGAKPVVVLQKLSLDDVQKLIKDREDKSRSSLKPIKNKPSKSNKGSIDQSVLKELPPELLAEIESTMPLCERVKMNKRKRSTVNEKPKYAEISSDEDNDSDETFESSRKRHKKDDDKAWEYEERDRRSSGDHRRSGHSHEGRRSSGGGRYRNRSPSDSDMEDYSPPPSLSEVARKMKKKEKQKKRKAYEPKLTPEEMMDSSTFKRFTASIENILDNLEDMDFTAFGDDDEIPQELLLGKHQLNELGSESAKIKAMGIMDKLSTDKTVKVLNILEKNIQDGSKLSTLLNHNNDTEEEERLWRDLIMERVTKSADACLTTINIMTSPNMPKAVYIEDVIERVIQYTKFHLQNTLYPQYDPVYRLDPHGGGLLSSKAKRAKCSTHKQRVIVMLYNKVCDIVSSLSELLEIQLLTDTTILQVSSMGITPFFVENVSELQLCAIKLVTAVFSRYEKHRQLILEEIFTSLARLPTSKRSLRNFRLNSSDMDGEPMYIQMVTALVLQLIQCVVHLPSSEKDSNSEEDSNKKVDQDVIITNSYETAMRTAQNFLSIFLKKCGSKQGEEDYRPLFENFVQDLLSTVNKPEWPAAELLLSLLGRLLVHQFSNKSTEMALRVASLDYLGTVAARLRKDAVTSKMDQGSIERILKQVSGGEDEIQQLQKALLDYLDENTETDPSLVFSRKFYIAQWFRDTTLETEKAMKSQKDEESSEGSHHAKEVETTGQIMHRAENRKKFLRSIIKTTPSQFSTLKMNSDTVDYDDACLIVRYLASMRPFAQSFDIYLTQILRVLGENAIAVRTKAMKCLSEVVAVDPSILARLDMQRGVHGRLMDNSTSVREAAVELLGRFVLCRPQLAEQYYDMLIERILDTGISVRKRVIKILRDICIEQPTFPKITEMCVKMIRRVNDEEGIKKLVNETFQKLWFTPTPHNDKEAMTRKILNITDVVAACRDTGYDWFEQLLQNLLKSEEDSSYKPVKKACTQLVDNLVEHILKYEESLADSDNKGVNSGRLVACITTLFLFSKIRPQLMVKHAMTMQPYLTTKCSTQNDFMVICNVAKILELVVPLMEHPSETFLATIEEDLMKLIIKYGMTVVQHCVSCLGAVVNKVTQNFKFVWACFNRYYGAISKLKSQHQEDPNNTSLLTNKPALLRSLFTVGALCRHFDFDLEDFKGNSKVNIKDKVLELLMYFTKHSDEEVQTKAIIGLGFAFIQHPSLMFEQEVKNLYNNILSDKNSSVNLKIQVLKNLQTYLQEEDTRMQQADRDWKKVAKQEDLKEMGDVSSGMSSSIMQLYLKQVLEAFFHTQSSVRHFALNVIALTLNQGLIHPVQCVPYLIAMGTDPEPAMRNKADQQLVEIDKKYAGFIHMKAVAGMKMSYQVQQAINTCLKDPVRGFRQDESSSALCSHLYSMIRGNRQHRRAFLISLLNLFDDTAKTEVNMLLYIADNLACFPYQTQEEPLFIMHHIDITLSVSGSNLLQSFKESMVKDKRKERKSSPGKENESSESEEEVSRPRKSRKRVDSDSDSDSEDDINSVMKCLPENSAPLIEFANVSQGILLLLMLKQHLKNLCGFSDSKIQKYSPSESAKVYDKAINRKTGVHFHPKQTLDFLRSDMANSKITEDVKRSIVKQYLDFKLLMEHLDPDEEEEEGEVSASTNARNKAITSLLGGGSPKNNAAAETEDDESDGEDRGGGTSGVRRRRSQRISQRITH

Target ORF information:

RefSeq Version	XM_005221614.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Nipped-B homolog (Drosophila) (NIPBL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005221614.2

ORF Insert Sequence:

ATGAATGGGG ATATGCCCCA TGTCCCCATC ACTACTCTTG CGGGGATTGC TAGTCTTACA 
GACCTCCTGA ACCAGCTGCC TCTTCCATCC CCTTTACCTG CTACAACTAC AAAGAGCCTT 
CTCTTTAATG CACGAATAGC AGAAGAAGTG AACTGCCTTT TGGCCTGTAG GGATGATAAT 
TTGGTTTCAC AGCTTGTCCA CAGCCTCAAC CAGGTATCAA CAGATCACAT AGAGTTGAAA 
GATAACCTTG GCAGTGATGA TCCAGAAGGC GACATACCAG TCTTGTTGCA GGCCGTCCTG 
GCGAGGAGTC CTAACGTTTT CAGGGAGAAA AGTATGCAGA ACAGATATGT ACAAAGTGGA 
ATGATGATGT CTCAGTATAA ACTTTCTCAG AATTCCATGC ACAGTAGTCC TGCATCTTCC 
AATTATCAAC AAACCACTAT CTCACATAGC CCCTCCAGCC GGTTTGTGCC ACCACAGACA 
AGCTCTGGGA ACAGATTTAT GCCACAACAA AATAGCCCAG TGCCTAGTCC ATATGCCCCA 
CAAAGCCCTG CAGGATACAT GCCATATTCC CATCCTTCAA GTTATACAAC ACATCCACAG 
ATGCAGCAAG CATCGGTATC AAGTCCCATT GTTGCAGGTG GTTTGAGAAA CCTACATGAT 
AATAAAGTTT CTGGTTCGTT GTCTGGCAAT TCTGCTAATC ATCATGCTGA TAATCCTAGA 
CATGGCTCAA GTGACGACTA CCTACACATG GTGCACAGGA TAAGTAGTGA CGATGGAGAT 
TCTTCAACAA TGAGGAATGC TGCGTCTTTT CCCTTGAGGT CTCCACAGCC AGTATGTTCC 
CCTGCTGGAA GTGATGGGAC TCCAAAAGGA TCAAGACCAT CTTTAATCCT GCAGTCTCAG 
TCTCTACCTT GTTCATCACC TCGAGATGTT CCACCAGACA TCTTGTTAGA TTCTCCAGAA 
AGAAAGCAAA AGAAGCAAAA GAAAATGAAA TTAGGCAAAG ATGAAAAAGA CCAGAATGAG 
AAGGCTGCAA TGTATGATAT CATTAGCTCT CCATCCAAGG ACTCCACTAA ACTTACGTTA 
AGACTTACTC GTGTAAGATC TTCAGACATG GACCAGCAAG AGGATATGCT TTCTGGTATG 
GAAAATAGCA ATGTTTCAGA AAATGATATT CCTTTTAATG TGCAGTACCC AGGACAGACT 
TCAAAAACAC CCATTACTCC ACAGGATGTA AACCGCCCAC TAAATGCTGC TCAGTGTTTG 
TCGCAGCAAG AACAAACAGC ATTCCTTCCA GCAAATCAAG TGCCTGTTTT ACAACAGAAC 
ACTTCAGTTG CTACAAAACA GCCCCAGACT TCTGTGGTAC TGAATCAGCA ACAGGTATCA 
CAACAGGGAC CTATATATGA TGAAGTGGAA TTGGATGCAT TGGCTGAAAT TGAGCGAATA 
GAGAGAGAAT CAGCTATTGA AAGGGAGCGT TTTTCAAAAG AAGTTCAAGA TAAAGATAAG 
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACAGGAGGT 
AATAGACCAG CTTCTCAGGA GACGGGTTCT ACGGGAAATG GGTCAAGGCC AGCATTAATG 
GTTAGCATTG ATCTTCATCA GGCAGGAAGA GTGGACTCTC AGGCCTCTAT AACTCAGGAT 
TCAGACTCCA TTAAAAAGCC TGAAGAAATC AAACAATGTA ACGACACACC TATTTCTGTT 
CTTCAGGAAG ATATTATTGG AAATCTTAAA TCTACACCAG AAAACCATCC TGAGACTCCT 
AAAAAAAAGT CTGATCCTGA GCTTTTAAAG AGTGAAGTGA AACAGAATGA AAGTAGATTG 
GCAGAGTCTA AACCAAATGA AAACAGATTG GTAGAGACAA AATCTAGTGA AACTAAGTTA 
GAAACTAAAA TTGAGGTACC AACAGAAGAA CCTAAACAGA ATGAGAACAG AATGATTGAA 
TCCAGACAAA ATGAGAGCAG CACAGCCGAG CCTAAACAAA ATGAAAATAG ACTGCCGGAC 
ACAAAACCAA ATGACAACAA ACAAAATAAT GGCAGATCAG AAACAACAAA ATCAAGACCC 
GAAACCCCAA AGCAAAAGGG TGAAAGCCGA CCTGAAACTC CAAAACAAAA GAGTGATGGG 
CGTCCTGAAA CCCCCAAACA AAAGGGTGAT GGACGGCCTG AAACTCCAAA GCAGAAAGGT 
GAGGGCCGAC CTGAAACTCC AAAGCAAAAA AATGAAGGTC GGCCTGAAAC ACCGAAACAT 
AGACATGAAA ATAGGAGGGA TTCTGGAAAG CCATCAACAG AGAAAAAACC TGAAGTGTCT 
AAACATAAAC AGGACATTAA ATCTGATTCA CCTCGGTTAA AATCAGAAAG AGCTGAAGCC 
TTAAAGCAGA GACCTGATGG GCGATCCATC TCTGAGTCAC TAAGACGTGA TCATGACAGT 
AAACAAAAGT CAGATGACAG GAGTGAATCT GAACGACATC GAGGGGATCA ATCTAGGGTT 
CGAAGACCAG AAACATTGAG ATCCTCTAGT AGAAATGACC ATGGCATTAA ATCTGATGGT 
TCAAAACCTG ATAAACTAGA AAGAAAACAC AGACATGAAT CAGGGGACTC AAGGGAAAGA 
CCATCTTCTG GGGAACAAAA GTCAAGACCT GACAGTCCTC GTGTTAAACA AGGAGATTCT 
AATAAATCAA GACCTGATAA GCCTGGTTTT AAATCTCCAA ATAGTAAGGA TGACAGAAGG 
ACAGAGGGTA ACAAGAGTAA AGTAGACAGT AATAAAACGC ACCCTGATAA TAAGGCAGAA 
TTTCCAAGTT ATTTGCTGGG GGGCAGGTCT GGTGCGTTGA AAAATTTTGT CATTCCAAAA 
ATCAAGAGGG ATAAAGATGG CAATATTACT CAGGAGACAA AGAAAATGGA AATGAAAGGA 
GAGCAGAAAG ACAAAGTAGA AAAAATGGGA TTAGTTGAAG ATCTAAATAA AGGAGCTAAG 
CCTGTAGTTG TCCTGCAAAA ACTGTCTTTG GATGATGTTC AAAAACTTAT TAAAGATAGA 
GAGGATAAAT CAAGAAGTTC CCTTAAACCT ATCAAGAATA AACCATCAAA GTCAAATAAA 
GGTAGTATAG ATCAATCAGT GTTAAAAGAA TTACCCCCTG AACTCCTGGC AGAAATTGAA 
TCCACCATGC CACTTTGTGA ACGTGTGAAA ATGAATAAAC GCAAGCGTAG CACAGTTAAT 
GAAAAACCAA AATATGCTGA AATCAGTTCA GATGAAGATA ATGATAGTGA TGAAACTTTT 
GAATCCTCTA GGAAACGACA TAAAAAAGAT GATGATAAAG CTTGGGAATA TGAAGAGCGT 
GACAGAAGAA GCTCTGGGGA TCATAGGAGA AGTGGCCACT CTCATGAAGG AAGAAGAAGT 
TCAGGTGGTG GTCGTTATCG AAACCGAAGC CCATCAGATT CTGACATGGA AGATTATTCT 
CCTCCTCCCA GCCTTAGTGA GGTCGCTAGA AAAATGAAGA AAAAAGAAAA ACAGAAGAAG 
CGGAAAGCTT ATGAACCAAA ACTAACACCC GAAGAAATGA TGGATTCTTC AACTTTTAAG 
AGATTCACAG CCTCAATAGA GAATATTTTG GATAACTTAG AAGATATGGA TTTTACTGCA 
TTTGGTGATG ATGATGAAAT TCCTCAGGAA CTGCTCTTAG GAAAACATCA GCTTAATGAA 
CTTGGCAGTG AATCTGCTAA AATTAAAGCA ATGGGTATAA TGGATAAGCT TTCCACTGAC 
AAAACTGTGA AAGTCCTAAA TATCTTGGAA AAGAATATTC AAGATGGGTC AAAGCTCTCC 
ACTTTGTTAA ATCATAATAA TGATACTGAA GAAGAAGAAA GGTTATGGAG AGACCTTATT 
ATGGAGAGAG TGACAAAATC AGCAGATGCC TGTCTTACAA CTATAAACAT CATGACATCC 
CCTAACATGC CAAAAGCTGT ATATATTGAG GATGTAATCG AAAGAGTTAT ACAGTACACT 
AAATTTCATT TGCAGAATAC ACTTTATCCT CAGTATGATC CTGTTTACAG ATTAGATCCT 
CATGGAGGAG GCTTACTAAG TTCAAAGGCA AAACGGGCTA AATGTTCTAC CCATAAGCAG 
AGAGTAATAG TAATGCTTTA TAATAAAGTT TGTGACATTG TTAGCAGCTT ATCAGAACTA 
CTAGAAATAC AACTTCTAAC AGACACAACA ATCCTTCAGG TATCTTCAAT GGGAATAACA 
CCATTTTTTG TGGAAAATGT CAGTGAACTA CAATTGTGTG CCATTAAATT AGTTACTGCA 
GTATTCTCAA GATATGAAAA ACATAGGCAG TTAATTTTGG AAGAAATTTT TACTTCACTG 
GCAAGATTAC CTACCAGCAA GAGGAGCTTA AGGAACTTCA GGTTAAATAG TAGTGATATG 
GATGGAGAGC CTATGTATAT TCAGATGGTT ACAGCACTGG TTTTACAACT AATTCAGTGT 
GTAGTACACT TACCATCATC AGAGAAGGAC TCTAATTCAG AAGAGGACTC AAATAAAAAA 
GTTGATCAAG ATGTTATCAT TACCAATTCT TATGAAACAG CCATGCGAAC AGCCCAAAAC 
TTCCTTTCCA TCTTCCTTAA AAAATGTGGT AGTAAGCAAG GTGAAGAAGA TTACAGACCA 
CTATTTGAAA ATTTTGTTCA AGACCTTCTT TCAACAGTCA ATAAGCCTGA ATGGCCAGCT 
GCTGAACTAC TCCTTAGTTT GTTAGGGAGA CTGTTGGTTC ATCAGTTCAG TAATAAGTCA 
ACAGAGATGG CTTTAAGAGT GGCATCCCTT GATTACCTTG GAACTGTCGC TGCACGACTG 
AGGAAAGATG CTGTTACAAG CAAAATGGAT CAAGGGTCTA TAGAACGAAT TTTAAAACAG 
GTTTCAGGAG GGGAAGATGA AATACAGCAA TTACAAAAAG CATTGCTTGA TTACTTGGAT 
GAAAACACGG AGACTGATCC TTCACTGGTG TTTTCTCGTA AATTCTATAT TGCCCAGTGG 
TTCCGTGATA CAACACTGGA AACAGAAAAA GCAATGAAAT CACAAAAAGA TGAAGAATCA 
TCTGAAGGAT CACATCATGC AAAGGAAGTT GAGACAACTG GTCAAATTAT GCATCGAGCT 
GAAAACCGGA AGAAGTTTCT TAGAAGCATT ATCAAAACCA CACCTTCTCA GTTCAGCACA 
TTAAAAATGA ACTCTGATAC TGTGGACTAT GATGATGCTT GTTTGATTGT TCGATACTTG 
GCCTCTATGA GGCCGTTTGC CCAGAGCTTT GATATTTATT TGACACAGAT CCTTCGAGTC 
CTTGGTGAAA ATGCAATTGC CGTTCGAACA AAAGCCATGA AGTGTTTATC TGAGGTTGTT 
GCTGTAGACC CCAGTATTCT AGCAAGGCTG GATATGCAGC GAGGAGTTCA TGGACGATTA 
ATGGATAACT CCACTAGTGT CAGAGAAGCA GCAGTGGAAT TACTAGGTCG ATTTGTCCTT 
TGTCGACCTC AGCTTGCTGA GCAGTATTAT GATATGCTGA TTGAAAGAAT ATTGGATACT 
GGTATCAGTG TCAGAAAAAG GGTGATAAAG ATTCTAAGAG ACATCTGTAT TGAACAACCG 
ACATTTCCAA AAATTACAGA AATGTGTGTA AAAATGATTC GCAGGGTAAA TGATGAAGAG 
GGCATTAAGA AATTAGTAAA TGAAACATTC CAAAAACTCT GGTTTACTCC AACTCCACAC 
AATGACAAAG AAGCAATGAC AAGGAAGATT TTAAATATCA CTGATGTGGT TGCAGCATGC 
AGAGATACTG GATATGACTG GTTTGAGCAG CTTCTTCAAA ATTTGTTGAA GTCTGAAGAG 
GATTCCTCAT ATAAACCTGT GAAGAAAGCT TGTACTCAAC TTGTTGATAA CCTAGTTGAG 
CACATTCTTA AATATGAGGA ATCTCTAGCT GACTCTGACA ATAAAGGTGT GAATTCTGGA 
AGATTGGTAG CTTGCATAAC CACTTTGTTC TTATTCAGCA AAATAAGACC CCAGCTCATG 
GTTAAACATG CCATGACTAT GCAGCCATAT CTTACCACTA AATGTAGTAC ACAAAATGAT 
TTCATGGTTA TCTGCAATGT TGCAAAAATT CTAGAACTAG TTGTACCACT GATGGAACAT 
CCGAGTGAAA CTTTCCTTGC CACTATTGAA GAAGATCTAA TGAAGCTTAT CATCAAATAT 
GGCATGACTG TAGTGCAACA TTGTGTAAGC TGTCTTGGGG CTGTTGTAAA TAAAGTGACA 
CAAAATTTTA AATTTGTGTG GGCCTGTTTC AATAGATACT ATGGTGCCAT TTCAAAATTA 
AAAAGTCAAC ACCAAGAGGA CCCAAATAAC ACTTCACTGC TAACAAACAA ACCAGCACTT 
TTGAGATCCC TATTCACTGT TGGGGCACTG TGTCGGCATT TTGATTTTGA TCTGGAAGAT 
TTTAAAGGCA ACAGTAAGGT TAACATAAAG GATAAAGTAC TTGAACTATT GATGTATTTT 
ACAAAGCATT CAGATGAAGA AGTGCAAACA AAAGCCATCA TTGGTCTAGG ATTTGCCTTT 
ATTCAGCACC CAAGTCTAAT GTTTGAGCAA GAGGTGAAAA ATCTATACAA TAATATTTTA 
TCTGATAAAA ACTCCTCAGT AAATTTAAAA ATACAAGTGT TAAAAAACCT CCAGACCTAC 
CTACAAGAAG AAGATACACG TATGCAGCAG GCAGATAGAG ACTGGAAGAA GGTTGCAAAA 
CAGGAAGATT TGAAAGAAAT GGGTGATGTT TCCTCAGGGA TGAGTAGTTC CATCATGCAG 
CTTTACCTCA AACAGGTGCT TGAGGCGTTT TTTCACACCC AATCAAGTGT ACGCCACTTC 
GCCCTCAATG TCATTGCATT GACTCTAAAC CAAGGTCTTA TTCATCCAGT TCAGTGTGTG 
CCGTATTTAA TTGCTATGGG CACAGACCCA GAACCAGCTA TGCGGAACAA GGCTGATCAG 
CAGCTTGTGG AAATAGACAA AAAATATGCT GGATTCATTC ACATGAAAGC AGTGGCTGGC 
ATGAAGATGT CTTACCAGGT ACAACAGGCA ATCAACACGT GCCTAAAAGA TCCTGTAAGG 
GGTTTCCGGC AGGACGAGTC CTCCAGTGCT TTGTGTTCAC ACCTTTACTC CATGATCCGT 
GGAAACCGCC AGCACAGACG CGCCTTTCTG ATTTCTTTAC TCAACCTCTT TGATGACACA 
GCAAAAACAG AAGTGAATAT GCTCTTGTAT ATAGCAGACA ATCTAGCCTG TTTTCCATAC 
CAGACACAGG AAGAGCCGTT GTTTATAATG CATCATATAG ACATTACACT CTCTGTTTCT 
GGTAGTAACC TACTGCAGTC ATTCAAGGAG TCCATGGTAA AAGACAAAAG GAAAGAGAGA 
AAATCATCTC CTGGTAAGGA AAATGAGTCA AGCGAGAGTG AAGAAGAAGT CTCTAGGCCT 
CGAAAGTCAC GGAAACGTGT AGATTCAGAT TCAGATTCGG ATTCAGAAGA TGATATAAAT 
TCGGTGATGA AATGTTTGCC AGAAAATTCA GCTCCTTTAA TTGAGTTTGC AAATGTCTCC 
CAGGGTATTT TATTGCTTCT GATGTTAAAG CAACATTTGA AGAATCTCTG TGGATTTTCT 
GATAGTAAAA TTCAGAAATA CTCTCCATCC GAATCTGCAA AAGTGTATGA TAAAGCGATA 
AACCGGAAAA CAGGAGTTCA TTTTCATCCC AAGCAAACAC TGGACTTCCT GCGGAGTGAT 
ATGGCTAATT CCAAAATCAC TGAAGATGTG AAGAGGAGTA TAGTAAAACA GTACCTAGAT 
TTCAAACTTC TCATGGAACA TCTGGACCCT GATGAAGAGG AAGAAGAAGG GGAGGTGTCA 
GCCAGCACAA ATGCTCGGAA CAAAGCAATT ACCTCGCTGC TTGGAGGAGG CAGCCCTAAA 
AATAATGCAG CAGCAGAGAC AGAAGATGAT GAAAGTGATG GGGAGGATAG AGGAGGAGGC 
ACTTCAGGGG TGAGGCGGAG GAGGAGTCAA CGTATTTCGC AGCGTATTAC TCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Marçais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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NIPBL cDNA ORF clone, Bos taurus(cattle)

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Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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