CACNA1F cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	CACNA1F
Entrez Gene ID	509779
Full Name	calcium voltage-gated channel subunit alpha1 F
General protein information	Preferred Names calcium voltage-gated channel subunit alpha1 F Names voltage-dependent L-type calcium channel subunit alpha-1F
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001205663.1	NP_001192592.1	voltage-dependent L-type calcium channel subunit alpha-1F
XM_024987854.1	XP_024843622.1	voltage-dependent L-type calcium channel subunit alpha-1F isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	CACNA1F	NP_005174.2
Macaca mulatta (Rhesus monkey)	CACNA1F	XP_001106163.2
Canis lupus familiaris (dog)	CACNA1F	XP_005642146.1
Danio rerio (zebrafish)	LOC559964	XP_003199134.2
Bos taurus (cattle)	CACNA1F	NP_001192592.1
Mus musculus (house mouse)	Cacna1f	NP_062528.2
Danio rerio (zebrafish)	LOC793708	XP_001333514.5
Xenopus tropicalis (tropical clawed frog)	cacna1f	XP_002941018.2
Rattus norvegicus (Norway rat)	Cacna1f	NP_446153.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Marçais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CACNA1F gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CACNA1F cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa37783	NM_001205663.1 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 F (CACNA1F), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa238313	XM_024987854.1 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 F (CACNA1F), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CACNA1F ( NM_001205663.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001192592.1 Bos taurus calcium voltage-gated channel subunit alpha1 F (CACNA1F), mRNA.

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CloneID	OBa37783
Clone ID Related Accession (Same CDS sequence)	NM_001205663.1
Accession Version	NM_001205663.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5919bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1527436800000
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1F
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000030.1. On Apr 20, 2011 this sequence version replaced XM_586812.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145425, SAMN03145431 [ECO:0000350] ##Evidence-Data-END##

ATGTCGGCAT CTGAAGGCGG GAAAGACACC ACCCCAGAGC CCAGTCCAGC CAATGGGACA 
GGCCCTGGGC CCGAATGGGG GCTGTGCCCT GGGCCCCCAG CATCAGATGG TGAAATCAGC 
GGGGCATCGG GCCTAGGGAC CCCTAAGCGA AAGACTCAGC ACAGTAAGCA CAAGACAGTG 
GCAGTAGCCA GTGCCCAGCG GTCACCCCGG GCACTCTTCT GCCTCACCCT GGCAAACCCC 
CTGCGGCGGT CCTGCATCAG CATTGTGGAA TGGAAGCCCT TCGACATCCT CATCTTGCTG 
ACCATCTTTG CCAATTGCGT GGCCCTGGGA GTCTACATCC CCTTCCCGGA GGACGACTCC 
AACACTGCCA ACCACAACCT GGAGCAAGTG GAATACGTAT TTTTGGTGAT TTTCACTGTG 
GAGACGGTGC TCAAGATCGT GGCCTATGGA CTGGTGCTCC ACCCCAGCGC CTACATCCGC 
AACGGCTGGA ACCTGCTCGA CTTCATCATT GTCGTGGTCG GGCTGTTCAG CGTGCTGCTG 
GAGCAGGGTC CCGGACGCCC AGGGGACACC CCGCATATGG GAGGGAAGCC AGGGGGCTTC 
GATGTGAAGG CATTGCGGGC ATTTCGGGTG CTGCGGCCAC TGAGGCTGGT GTCTGGGGTC 
CCAAGCCTGC ACATAGTGCT CAATTCCATC ATGAAGGCTC TGGTGCCGCT GTTGCATATT 
GCACTTCTTG TGCTCTTTGT CATCATCATT TACGCCATCA TTGGACTGGA GCTGTTCCTC 
GGACGCATGC ACAAAACGTG CTACTTCCTG GGATCTGACG TAGAGGCGGA GGAGGACCCG 
TCACCCTGTG CGTCCTCAGG ATCAGGGCGT GCTTGCACAC TGAACCAGAC GGAGTGCCGC 
GGGCGCTGGG CAGGGCCCAA CGGAGGCATC ACCAACTTTG ACAACTTTTT CTTCGCCATG 
CTGACAGTCT TCCAGTGCAT CACCATGGAA GGCTGGACCG ACGTGCTCTA CTGGATGCAG 
GATGCCATGG GGTATGAGCT GCCCTGGGTG TATTTTGTGA GTCTCGTCAT CTTTGGATCC 
TTCTTCGTCC TCAACCTTGT GCTTGGTGTC CTGAGTGGGG AGTTCTCCAA AGAGAGGGAG 
AAGGCAAAAG CACGAGGGGA TTTCCAGAAG CTTCGGGAGA AGCAGCAGCT GGAGGAGGAC 
CTCCGGGGCT ACCTGGACTG GATCACACAG GCGGAGGAGC TGGACATGGA GGACCCCTCA 
GCTGCTGGCA ACTTTGGTTC CGTGGCTGAA GAGGGCCTGG CCGGCCACCG GCCACAACTG 
GAAGAGCTGA CCAATAGGAG ACGAGGACGT CTACGCTGGT TCAGTCACTC CACCCACTCC 
ACCAGCAGCC ATGCCAGCCT CCCAGCCAGT GACACCGGTT CAATGGCAGA GACCCAAGGA 
GACGAGGAAG AGGAAGAGGG CACTGTAGCC AGCTGTACAC GCTGCCTAAA TAAGATCGTG 
AAAACCAGCG TTTGCCGCCG TCTCCGCCGA GCCAACCGGG GCCTTCGGGC GAGCTGCCGG 
AGGGCTGTGA AGTCCAATGC CTGCTACTGG GCCGTGTTGC TGCTCGTCTT CCTCAACACT 
CTGACAATCG CCTCGGAGCA CCACGGGCAG CCCTTCTGGC TCACCCAGAT CCAGGAGTAT 
GCCAACAAAG TGCTACTCTG TCTATTCACG GTGGAGATGC TTCTCAAGTT GTATGGTCTG 
GGGCCCTCTG TCTACGTGTC CTCCTTCTTC AACCGCTTTG ACTGCTTCGT GGTCTGTGGG 
GGCATCCTGG AGACTACCCT GGTGGAGGTG GGTGCCATGC AGCCCCTGGG CATCTCGGTA 
CTCCGCTGTG TGCGCCTCCT CAGGATCTTT AAGGTCACCA GGCACTGGGC ATCTCTGAGC 
AATTTAGTGG CGTCTCTACT CAATTCAATG AAATCCATTG CATCCTTGCT GCTTCTCCTC 
TTCCTCTTTA TCATCATCTT CTCCCTGCTT GGCATGCAGC TGTTTGGGGG CAAGTTCAAC 
TTTGACCAGA CCCACACCAA GCGAAGCACC TTTGACACCT TTCCCCAGGC CCTCCTCACT 
GTCTTTCAGA TACTGACGGG TGAGGACTGG AATGTGGTCA TGTATGATGG TATCATGGCC 
TATGGCGGCC CCTTCTTCCC AGGGATGCTC GTGTGCGTGT ATTTCATCAT CCTCTTCATC 
TGCGGCAATT ACATCCTATT GAATGTGTTT CTTGCCATCG CTGTGGACAA CCTGGCCAGT 
GGAGATGCAG GCACTGACAA AGATAAGGGC AGGGAGAAGA TCACTGAGGA AACTCCACAG 
GAGAATGGAG CACTGGTGCC TGGTGGGGAG AATGAGGAAG AGGAATCTGC AAAAAATGAA 
GGAGCAGGCA AGTGGGCAGG CATGGAGGAA GAGGAGGAGG AGGAGGAGGA GGAAGAGGAA 
GTGGGGGCCG GGCATGTGGA ACTCCTGCAG GAAGTTGTAC CCAAGGAGAA GGTGGTACCC 
ATCCCTGAGG GCAGTGCTTT CTTCTGCCTC AGCCAAACCA ACCCGCTGAG GAAGGCCTGC 
CACACCCTCA TCCACCATCA CATCTTCACC AATCTCATCC TGGTGTTTAT CATCCTCAGC 
AGTGTGTCCC TGGCCGCTGA GGATCCCATC CGAGCCCACT CCTTCCGCAA CCACATTCTG 
GGGTACTTTG ATTATGCCTT CACCTCCATT TTCACTGTGG AGATTCTACT AAAGATGACA 
GTGTTCGGGG CTTTCCTGCA CCGTGGTTCC TTCTGCCGTA GCTGGTTCAA TCTGCTGGAT 
CTGCTGGTGG TCAGCGTGTC CCTCATCTCC TTTGGTATCC ACTCCAGCGC CATCTCAGTG 
GTGAAGATTC TGCGTGTGCT CCGAGTACTG CGGCCCCTTC GAGCTATCAA CAGGGCCAAA 
GGACTCAAGC ATGTGGTGCA ATGTGTGTTC GTGGCCATCC GGACCATCGG AAATATCATG 
ATCGTGACCA CGCTCCTGCA GTTCATGTTC GCCTGCATTG GTGTGCAGCT CTTCAAGGGG 
AAATTCTACA GTTGCACTGA TGAGGCCAAA CACACACCCC AAGAATGCAA GGGCTCCTTC 
CTGGTCTACC CCGATGGAGA TGTGTCAAGG CCTCTGGTCC GGGAGCGGCT CTGGGTCAAC 
AGCGATTTCA ACTTTGACAA TGTCCTTTCA GCCATGATGG CCCTGTTCAC TGTCTCCACC 
TTCGAAGGCT GGCCTGCGCT GCTATACAAG GCCATTGATG CAAACGCAGA GGACAAGGGC 
CCCATCTACA ATTACCACGT GGAGATCTCA GTGTTCTTCA TTGTCTATAT CATCATCATT 
GCATTCTTCA TGATGAACAT CTTTGTGGGC TTTGTCATCA TCACCTTCCG AGCCCAGGGC 
GAGCAGGAGT ACCAAGACTG TGAGCTGGAT AAGAACCAGC GCCAGTGTGT AGAATACGCC 
CTCAAGGCCC AACCACTCCG TCGCTATATC CCCAAGAACC CACATCAGTA TCGTGTGTGG 
GCCACTGTGA ACTCTGCTGC CTTCGAGTAT CTCATGTTCC TGCTCATCCT GCTCAACACG 
GTTGCTCTAG CCATGCAGCA CTATGAGCAG ACTGCTCCCT TCAACTATGC CATGGACATC 
CTCAACATGG TCTTCACTGG CCTCTTCACT GTCGAGATGG TGCTCAAAAT CATTGCCTTC 
AAACCCAAGC ATTACTTTAC TGATGCCTGG AACACATTTG ATGCTCTTAT TGTGGTGGGC 
AGCGTAGTGG ACATTGCCGT CACTGAAGTC AACAATGGTG GCCACCTGGG CGAGAGCTCT 
GAGGACAGTT CCCGCATTTC GATCACCTTC TTTCGCCTCT TCCGAGTCAT GCGATTGGTC 
AAGCTTCTTA GTAAGGGTGA AGGGATTCGC ACATTACTTT GGACATTCAT CAAGTCTTTT 
CAGGCCTTAC CCTATGTGGC TCTTCTCATC GCAATGATAT TCTTCATTTA CGCAGTCATT 
GGGATGCAGA TGTTTGGCAA GGTGGCTCTT CAGGATGGCA CACAGATCAA CCGAAACAAC 
AACTTCCAGA CCTTTCCACA GGCTGTACTG CTTTTGTTCA GGTGTGCTAC TGGTGAGGCA 
TGGCAGGAGA TAATGCTTGC CAGCCTTCCT GGAAGCCGGT GTGACCCTGA GTCTGACTTC 
GGCCCTGGTG AGGAGTTTAG CTGTGGTAGC AATTTTGCCA TCGCCTATTT TATCAGCTTC 
TTCATGCTCT GTGCATTCCT GATCATAAAT CTCTTTGTGG CTGTGATCAT GGACAACTTT 
GATTATCTAA CCAGAGATTG GTCCATCCTG GGCCCCCATC ACCTCGATGA ATTCAAGAGG 
ATTTGGTCTG AATATGACCC TGGGGCCAAA GGCCGCATCA AGCACCTGGA TGTGGTTGCC 
CTGCTGAGAC GCATCCAGCC CCCACTGGGA TTTGGGAAGC TGTGCCCACA CCGAGTGGCC 
TGCAAGAGAC TTGTGGCGAT GAACATGCCC CTCAACTCAG ATGGGACAGT GACATTCAAC 
GCCACACTAT TTGCCCTGGT CCGGACATCA CTGAAGATCA AGACGGAAGG AAACCTGGAG 
CAAGCCAATC AGGAGCTGCG GATTGTCATC AAAAAGATCT GGAAACGGAT GAAGCAGAAG 
CTGCTAGATG AGGTCATCCC ACCAGCTGAT GAGGAAGAAG TTACTGTGGG CAAATTCTAC 
GCCACATTTC TGATCCAGGA CTACTTCCGC AAATTCCGGC GGAGGAAAGA AAAGGGGCTA 
CTAGGCACCG AGGCCCCCCC AAGCACCTCC TCTGCCCTTC AGGCTGGCCT GAGGAGCCTG 
CAAGACTTGG GTCCGGAGAT GCGGCAGGCC CTCACTTGTG ACACAGAGGA AGAGGAAGGG 
GAAGAGGGGC ATGAGGGAGA GGAGGAACAT GAGGACCTGG AAACATACAC AGCCCTGATG 
GGCTCCCAGC CCCCATCTCG CCGGAGCTCC GTGATTTCTG TGTCTCTGCC TGCTGGAGAC 
AGACCTCCAG ATTCACTTTC CCCTGGACCC AGTGATGACG ATGGGGGGGC TCCCAACTCC 
AGACAGTCCA ATGTTCCCCA GTCTGGGTCC CATGCCCACA GGAGAAGCTC TGGGGTTCTC 
ATTTTCACCA TTCCAGAAGA AGGAAGTTCT CAGTCCAAGG CAACCAAAGG GCAAGAGAGG 
CAGGATGAGC AAGAGGAAGT CCTGAGCCAG CTCTCCTACC TAGATGAACA GGCAGGGGCT 
CCCCCACGCC CCATCCTTTT GCCATCTCAC AGACCCCAGA GATACGTGGA TGGCCACCAA 
GCACCACGCC GCCGTCTGCT GCCCCCAACG CCTGCAGGTC GGAAGCCATC CTTCAACATC 
CAGTGTCTGC GGCGCCAGGG CAGCTGTGAA GATTTACCCA TTCCAGGCAC CTATCATCGT 
GGACGAAACT CAGGGCCCAG TAGGGCACAG GGTTCCTGGG CAACCCCTCC TCAGCGGGGC 
CGACTCCTAT ATGCCCCACT GCTATTGGTG GAGGAGGGTG CAGCAGGGGA GGGATACATC 
GGCAAATCCA GCAGTCCGCT GCACACCTTC ACCTGTCTGC ATGTGCCTGG AACCCACTCG 
GACCCCAGCC ATGGCAAAAG GGGCAGTGCT GACAGCCTGG TGGAGGCTGT GCTCATCTCT 
GAGGGCCTGG GCCTCTTTGC TCGAGACCCA CGCTTCGTGG CCCTGGCCAA GCAGGAGATT 
GCAGATGCAT GTCGCCTGAC CCTGGACGAG ATGGACAGTG CTGCCAGTGA CCTGCTGGCA 
CAGGGGACCA GCTCACTGTA TAGTGACGAA GAGTCCATCC TCTCCCGTTT TGATGAGGAG 
GACCTAGGAG ACGAGATGGC CTGCGTCCAT GCCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192592.1
CDS	1..5919
Translation	MSASEGGKDTTPEPSPANGTGPGPEWGLCPGPPASDGEISGASGLGTPKRKTQHSKHKTVAVASAQRSPRALFCLTLANPLRRSCISIVEWKPFDILILLTIFANCVALGVYIPFPEDDSNTANHNLEQVEYVFLVIFTVETVLKIVAYGLVLHPSAYIRNGWNLLDFIIVVVGLFSVLLEQGPGRPGDTPHMGGKPGGFDVKALRAFRVLRPLRLVSGVPSLHIVLNSIMKALVPLLHIALLVLFVIIIYAIIGLELFLGRMHKTCYFLGSDVEAEEDPSPCASSGSGRACTLNQTECRGRWAGPNGGITNFDNFFFAMLTVFQCITMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLRGYLDWITQAEELDMEDPSAAGNFGSVAEEGLAGHRPQLEELTNRRRGRLRWFSHSTHSTSSHASLPASDTGSMAETQGDEEEEEGTVASCTRCLNKIVKTSVCRRLRRANRGLRASCRRAVKSNACYWAVLLLVFLNTLTIASEHHGQPFWLTQIQEYANKVLLCLFTVEMLLKLYGLGPSVYVSSFFNRFDCFVVCGGILETTLVEVGAMQPLGISVLRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDQTHTKRSTFDTFPQALLTVFQILTGEDWNVVMYDGIMAYGGPFFPGMLVCVYFIILFICGNYILLNVFLAIAVDNLASGDAGTDKDKGREKITEETPQENGALVPGGENEEEESAKNEGAGKWAGMEEEEEEEEEEEEVGAGHVELLQEVVPKEKVVPIPEGSAFFCLSQTNPLRKACHTLIHHHIFTNLILVFIILSSVSLAAEDPIRAHSFRNHILGYFDYAFTSIFTVEILLKMTVFGAFLHRGSFCRSWFNLLDLLVVSVSLISFGIHSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYSCTDEAKHTPQECKGSFLVYPDGDVSRPLVRERLWVNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDANAEDKGPIYNYHVEISVFFIVYIIIIAFFMMNIFVGFVIITFRAQGEQEYQDCELDKNQRQCVEYALKAQPLRRYIPKNPHQYRVWATVNSAAFEYLMFLLILLNTVALAMQHYEQTAPFNYAMDILNMVFTGLFTVEMVLKIIAFKPKHYFTDAWNTFDALIVVGSVVDIAVTEVNNGGHLGESSEDSSRISITFFRLFRVMRLVKLLSKGEGIRTLLWTFIKSFQALPYVALLIAMIFFIYAVIGMQMFGKVALQDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLASLPGSRCDPESDFGPGEEFSCGSNFAIAYFISFFMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPGAKGRIKHLDVVALLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVTFNATLFALVRTSLKIKTEGNLEQANQELRIVIKKIWKRMKQKLLDEVIPPADEEEVTVGKFYATFLIQDYFRKFRRRKEKGLLGTEAPPSTSSALQAGLRSLQDLGPEMRQALTCDTEEEEGEEGHEGEEEHEDLETYTALMGSQPPSRRSSVISVSLPAGDRPPDSLSPGPSDDDGGAPNSRQSNVPQSGSHAHRRSSGVLIFTIPEEGSSQSKATKGQERQDEQEEVLSQLSYLDEQAGAPPRPILLPSHRPQRYVDGHQAPRRRLLPPTPAGRKPSFNIQCLRRQGSCEDLPIPGTYHRGRNSGPSRAQGSWATPPQRGRLLYAPLLLVEEGAAGEGYIGKSSSPLHTFTCLHVPGTHSDPSHGKRGSADSLVEAVLISEGLGLFARDPRFVALAKQEIADACRLTLDEMDSAASDLLAQGTSSLYSDEESILSRFDEEDLGDEMACVHAL

Target ORF information:

RefSeq Version	NM_001205663.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 F (CACNA1F), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001205663.1

ORF Insert Sequence:

ATGTCGGCAT CTGAAGGCGG GAAAGACACC ACCCCAGAGC CCAGTCCAGC CAATGGGACA 
GGCCCTGGGC CCGAATGGGG GCTGTGCCCT GGGCCCCCAG CATCAGATGG TGAAATCAGC 
GGGGCATCGG GCCTAGGGAC CCCTAAGCGA AAGACTCAGC ACAGTAAGCA CAAGACAGTG 
GCAGTAGCCA GTGCCCAGCG GTCACCCCGG GCACTCTTCT GCCTCACCCT GGCAAACCCC 
CTGCGGCGGT CCTGCATCAG CATTGTGGAA TGGAAGCCCT TCGACATCCT CATCTTGCTG 
ACCATCTTTG CCAATTGCGT GGCCCTGGGA GTCTACATCC CCTTCCCGGA GGACGACTCC 
AACACTGCCA ACCACAACCT GGAGCAAGTG GAATACGTAT TTTTGGTGAT TTTCACTGTG 
GAGACGGTGC TCAAGATCGT GGCCTATGGA CTGGTGCTCC ACCCCAGCGC CTACATCCGC 
AACGGCTGGA ACCTGCTCGA CTTCATCATT GTCGTGGTCG GGCTGTTCAG CGTGCTGCTG 
GAGCAGGGTC CCGGACGCCC AGGGGACACC CCGCATATGG GAGGGAAGCC AGGGGGCTTC 
GATGTGAAGG CATTGCGGGC ATTTCGGGTG CTGCGGCCAC TGAGGCTGGT GTCTGGGGTC 
CCAAGCCTGC ACATAGTGCT CAATTCCATC ATGAAGGCTC TGGTGCCGCT GTTGCATATT 
GCACTTCTTG TGCTCTTTGT CATCATCATT TACGCCATCA TTGGACTGGA GCTGTTCCTC 
GGACGCATGC ACAAAACGTG CTACTTCCTG GGATCTGACG TAGAGGCGGA GGAGGACCCG 
TCACCCTGTG CGTCCTCAGG ATCAGGGCGT GCTTGCACAC TGAACCAGAC GGAGTGCCGC 
GGGCGCTGGG CAGGGCCCAA CGGAGGCATC ACCAACTTTG ACAACTTTTT CTTCGCCATG 
CTGACAGTCT TCCAGTGCAT CACCATGGAA GGCTGGACCG ACGTGCTCTA CTGGATGCAG 
GATGCCATGG GGTATGAGCT GCCCTGGGTG TATTTTGTGA GTCTCGTCAT CTTTGGATCC 
TTCTTCGTCC TCAACCTTGT GCTTGGTGTC CTGAGTGGGG AGTTCTCCAA AGAGAGGGAG 
AAGGCAAAAG CACGAGGGGA TTTCCAGAAG CTTCGGGAGA AGCAGCAGCT GGAGGAGGAC 
CTCCGGGGCT ACCTGGACTG GATCACACAG GCGGAGGAGC TGGACATGGA GGACCCCTCA 
GCTGCTGGCA ACTTTGGTTC CGTGGCTGAA GAGGGCCTGG CCGGCCACCG GCCACAACTG 
GAAGAGCTGA CCAATAGGAG ACGAGGACGT CTACGCTGGT TCAGTCACTC CACCCACTCC 
ACCAGCAGCC ATGCCAGCCT CCCAGCCAGT GACACCGGTT CAATGGCAGA GACCCAAGGA 
GACGAGGAAG AGGAAGAGGG CACTGTAGCC AGCTGTACAC GCTGCCTAAA TAAGATCGTG 
AAAACCAGCG TTTGCCGCCG TCTCCGCCGA GCCAACCGGG GCCTTCGGGC GAGCTGCCGG 
AGGGCTGTGA AGTCCAATGC CTGCTACTGG GCCGTGTTGC TGCTCGTCTT CCTCAACACT 
CTGACAATCG CCTCGGAGCA CCACGGGCAG CCCTTCTGGC TCACCCAGAT CCAGGAGTAT 
GCCAACAAAG TGCTACTCTG TCTATTCACG GTGGAGATGC TTCTCAAGTT GTATGGTCTG 
GGGCCCTCTG TCTACGTGTC CTCCTTCTTC AACCGCTTTG ACTGCTTCGT GGTCTGTGGG 
GGCATCCTGG AGACTACCCT GGTGGAGGTG GGTGCCATGC AGCCCCTGGG CATCTCGGTA 
CTCCGCTGTG TGCGCCTCCT CAGGATCTTT AAGGTCACCA GGCACTGGGC ATCTCTGAGC 
AATTTAGTGG CGTCTCTACT CAATTCAATG AAATCCATTG CATCCTTGCT GCTTCTCCTC 
TTCCTCTTTA TCATCATCTT CTCCCTGCTT GGCATGCAGC TGTTTGGGGG CAAGTTCAAC 
TTTGACCAGA CCCACACCAA GCGAAGCACC TTTGACACCT TTCCCCAGGC CCTCCTCACT 
GTCTTTCAGA TACTGACGGG TGAGGACTGG AATGTGGTCA TGTATGATGG TATCATGGCC 
TATGGCGGCC CCTTCTTCCC AGGGATGCTC GTGTGCGTGT ATTTCATCAT CCTCTTCATC 
TGCGGCAATT ACATCCTATT GAATGTGTTT CTTGCCATCG CTGTGGACAA CCTGGCCAGT 
GGAGATGCAG GCACTGACAA AGATAAGGGC AGGGAGAAGA TCACTGAGGA AACTCCACAG 
GAGAATGGAG CACTGGTGCC TGGTGGGGAG AATGAGGAAG AGGAATCTGC AAAAAATGAA 
GGAGCAGGCA AGTGGGCAGG CATGGAGGAA GAGGAGGAGG AGGAGGAGGA GGAAGAGGAA 
GTGGGGGCCG GGCATGTGGA ACTCCTGCAG GAAGTTGTAC CCAAGGAGAA GGTGGTACCC 
ATCCCTGAGG GCAGTGCTTT CTTCTGCCTC AGCCAAACCA ACCCGCTGAG GAAGGCCTGC 
CACACCCTCA TCCACCATCA CATCTTCACC AATCTCATCC TGGTGTTTAT CATCCTCAGC 
AGTGTGTCCC TGGCCGCTGA GGATCCCATC CGAGCCCACT CCTTCCGCAA CCACATTCTG 
GGGTACTTTG ATTATGCCTT CACCTCCATT TTCACTGTGG AGATTCTACT AAAGATGACA 
GTGTTCGGGG CTTTCCTGCA CCGTGGTTCC TTCTGCCGTA GCTGGTTCAA TCTGCTGGAT 
CTGCTGGTGG TCAGCGTGTC CCTCATCTCC TTTGGTATCC ACTCCAGCGC CATCTCAGTG 
GTGAAGATTC TGCGTGTGCT CCGAGTACTG CGGCCCCTTC GAGCTATCAA CAGGGCCAAA 
GGACTCAAGC ATGTGGTGCA ATGTGTGTTC GTGGCCATCC GGACCATCGG AAATATCATG 
ATCGTGACCA CGCTCCTGCA GTTCATGTTC GCCTGCATTG GTGTGCAGCT CTTCAAGGGG 
AAATTCTACA GTTGCACTGA TGAGGCCAAA CACACACCCC AAGAATGCAA GGGCTCCTTC 
CTGGTCTACC CCGATGGAGA TGTGTCAAGG CCTCTGGTCC GGGAGCGGCT CTGGGTCAAC 
AGCGATTTCA ACTTTGACAA TGTCCTTTCA GCCATGATGG CCCTGTTCAC TGTCTCCACC 
TTCGAAGGCT GGCCTGCGCT GCTATACAAG GCCATTGATG CAAACGCAGA GGACAAGGGC 
CCCATCTACA ATTACCACGT GGAGATCTCA GTGTTCTTCA TTGTCTATAT CATCATCATT 
GCATTCTTCA TGATGAACAT CTTTGTGGGC TTTGTCATCA TCACCTTCCG AGCCCAGGGC 
GAGCAGGAGT ACCAAGACTG TGAGCTGGAT AAGAACCAGC GCCAGTGTGT AGAATACGCC 
CTCAAGGCCC AACCACTCCG TCGCTATATC CCCAAGAACC CACATCAGTA TCGTGTGTGG 
GCCACTGTGA ACTCTGCTGC CTTCGAGTAT CTCATGTTCC TGCTCATCCT GCTCAACACG 
GTTGCTCTAG CCATGCAGCA CTATGAGCAG ACTGCTCCCT TCAACTATGC CATGGACATC 
CTCAACATGG TCTTCACTGG CCTCTTCACT GTCGAGATGG TGCTCAAAAT CATTGCCTTC 
AAACCCAAGC ATTACTTTAC TGATGCCTGG AACACATTTG ATGCTCTTAT TGTGGTGGGC 
AGCGTAGTGG ACATTGCCGT CACTGAAGTC AACAATGGTG GCCACCTGGG CGAGAGCTCT 
GAGGACAGTT CCCGCATTTC GATCACCTTC TTTCGCCTCT TCCGAGTCAT GCGATTGGTC 
AAGCTTCTTA GTAAGGGTGA AGGGATTCGC ACATTACTTT GGACATTCAT CAAGTCTTTT 
CAGGCCTTAC CCTATGTGGC TCTTCTCATC GCAATGATAT TCTTCATTTA CGCAGTCATT 
GGGATGCAGA TGTTTGGCAA GGTGGCTCTT CAGGATGGCA CACAGATCAA CCGAAACAAC 
AACTTCCAGA CCTTTCCACA GGCTGTACTG CTTTTGTTCA GGTGTGCTAC TGGTGAGGCA 
TGGCAGGAGA TAATGCTTGC CAGCCTTCCT GGAAGCCGGT GTGACCCTGA GTCTGACTTC 
GGCCCTGGTG AGGAGTTTAG CTGTGGTAGC AATTTTGCCA TCGCCTATTT TATCAGCTTC 
TTCATGCTCT GTGCATTCCT GATCATAAAT CTCTTTGTGG CTGTGATCAT GGACAACTTT 
GATTATCTAA CCAGAGATTG GTCCATCCTG GGCCCCCATC ACCTCGATGA ATTCAAGAGG 
ATTTGGTCTG AATATGACCC TGGGGCCAAA GGCCGCATCA AGCACCTGGA TGTGGTTGCC 
CTGCTGAGAC GCATCCAGCC CCCACTGGGA TTTGGGAAGC TGTGCCCACA CCGAGTGGCC 
TGCAAGAGAC TTGTGGCGAT GAACATGCCC CTCAACTCAG ATGGGACAGT GACATTCAAC 
GCCACACTAT TTGCCCTGGT CCGGACATCA CTGAAGATCA AGACGGAAGG AAACCTGGAG 
CAAGCCAATC AGGAGCTGCG GATTGTCATC AAAAAGATCT GGAAACGGAT GAAGCAGAAG 
CTGCTAGATG AGGTCATCCC ACCAGCTGAT GAGGAAGAAG TTACTGTGGG CAAATTCTAC 
GCCACATTTC TGATCCAGGA CTACTTCCGC AAATTCCGGC GGAGGAAAGA AAAGGGGCTA 
CTAGGCACCG AGGCCCCCCC AAGCACCTCC TCTGCCCTTC AGGCTGGCCT GAGGAGCCTG 
CAAGACTTGG GTCCGGAGAT GCGGCAGGCC CTCACTTGTG ACACAGAGGA AGAGGAAGGG 
GAAGAGGGGC ATGAGGGAGA GGAGGAACAT GAGGACCTGG AAACATACAC AGCCCTGATG 
GGCTCCCAGC CCCCATCTCG CCGGAGCTCC GTGATTTCTG TGTCTCTGCC TGCTGGAGAC 
AGACCTCCAG ATTCACTTTC CCCTGGACCC AGTGATGACG ATGGGGGGGC TCCCAACTCC 
AGACAGTCCA ATGTTCCCCA GTCTGGGTCC CATGCCCACA GGAGAAGCTC TGGGGTTCTC 
ATTTTCACCA TTCCAGAAGA AGGAAGTTCT CAGTCCAAGG CAACCAAAGG GCAAGAGAGG 
CAGGATGAGC AAGAGGAAGT CCTGAGCCAG CTCTCCTACC TAGATGAACA GGCAGGGGCT 
CCCCCACGCC CCATCCTTTT GCCATCTCAC AGACCCCAGA GATACGTGGA TGGCCACCAA 
GCACCACGCC GCCGTCTGCT GCCCCCAACG CCTGCAGGTC GGAAGCCATC CTTCAACATC 
CAGTGTCTGC GGCGCCAGGG CAGCTGTGAA GATTTACCCA TTCCAGGCAC CTATCATCGT 
GGACGAAACT CAGGGCCCAG TAGGGCACAG GGTTCCTGGG CAACCCCTCC TCAGCGGGGC 
CGACTCCTAT ATGCCCCACT GCTATTGGTG GAGGAGGGTG CAGCAGGGGA GGGATACATC 
GGCAAATCCA GCAGTCCGCT GCACACCTTC ACCTGTCTGC ATGTGCCTGG AACCCACTCG 
GACCCCAGCC ATGGCAAAAG GGGCAGTGCT GACAGCCTGG TGGAGGCTGT GCTCATCTCT 
GAGGGCCTGG GCCTCTTTGC TCGAGACCCA CGCTTCGTGG CCCTGGCCAA GCAGGAGATT 
GCAGATGCAT GTCGCCTGAC CCTGGACGAG ATGGACAGTG CTGCCAGTGA CCTGCTGGCA 
CAGGGGACCA GCTCACTGTA TAGTGACGAA GAGTCCATCC TCTCCCGTTT TGATGAGGAG 
GACCTAGGAG ACGAGATGGC CTGCGTCCAT GCCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1F ( XM_024987854.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024843622.1 Bos taurus calcium voltage-gated channel subunit alpha1 F (CACNA1F), transcript variant X1, mRNA.

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CloneID	OBa238313
Clone ID Related Accession (Same CDS sequence)	XM_024987854.1
Accession Version	XM_024987854.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5712bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1F isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCAT CTGAAGGCGG GAAAGACACC ACCCCAGAGC CCAGTCCAGC CAATGGGACA 
GGCCCTGGGC CCGAATGGGG GCTGTGCCCT GGGCCCCCAG CATCAGATGG TGAAATCAGC 
GGGGCATCGG GCCTAGGGAC CCCTAAGCGA AAGACTCAGC ACAGTAAGCA CAAGACAGTG 
GCAGTAGCCA GTGCCCAGCG GTCACCCCGG GCACTCTTCT GCCTCACCCT GGCAAACCCC 
CTGCGGCGGC CCTTCGACAT CCTCATCTTG CTGACCATCT TTGCCAATTG CGTGGCCCTG 
GGAGTCTACA TCCCCTTCCC GGAGGACGAC TCCAACACTG CCAACCACAA CCTGGAGCAA 
GTGGAATACG TATTTTTGGT GATTTTCACT GTGGAGACGG TGCTCAAGAT CGTGGCCTAT 
GGACTGGTGC TCCACCCCAG CGCCTACATC CGCAACGGCT GGAACCTGCT CGACTTCATC 
ATTGTCGTGG TCGGGCTGTT CAGCGTGCTG CTGGAGCAGG GTCCCGGACG CCCAGGGGAC 
ACCCCGCATA TGGGAGGGAA GCCAGGGGGC TTCGATGTGA AGGCATTGCG GGCATTTCGG 
GTGCTGCGGC CACTGAGGCT GGTGTCTGGG GTCCCAAGCC TGCACATAGT GCTCAATTCC 
ATCATGAAGG CTCTGGTGCC GCTGTTGCAT ATTGCACTTC TTGTGCTCTT TGTCATCATC 
ATTTACGCCA TCATTGGACT GGAGCTGTTC CTCGGACGCA TGCACAAAAC GTGCTACTTC 
CTGGGATCTG ACGTAGAGGC GGAGGAGGAC CCGTCACCCT GTGCGTCCTC AGGATCAGGG 
CGTGCTTGCA CACTGAACCA GACGGAGTGC CGCGGGCGCT GGGCAGGGCC CAACGGAGGC 
ATCACCAACT TTGACAACTT TTTCTTCGCC ATGCTGACAG TCTTCCAGTG CATCACCATG 
GAAGGCTGGA CCGACGTGCT CTACTGGATG CAGGATGCCA TGGGGTATGA GCTGCCCTGG 
GTGTATTTTG TGAGTCTCGT CATCTTTGGA TCCTTCTTCG TCCTCAACCT TGTGCTTGGT 
GTCCTGAGTG GGGAGTTCTC CAAAGAGAGG GAGAAGGCAA AAGCACGAGG GGATTTCCAG 
AAGCTTCGGG AGAAGCAGCA GCTGGAGGAG GACCTCCGGG GCTACCTGGA CTGGATCACA 
CAGGCGGAGG AGCTGGACAT GGAGGACCCC TCAGCTGCTG GCAACTTTGG TTCCGTGGCT 
GAAGAGGGCC TGGCCGGCCA CCGGCCACAA CTGGAAGAGC TGACCAATAG GAGACGAGGA 
CGTCTACGCT GGTTCAGTCA CTCCACCCAC TCCACCAGCA GCCATGCCAG CCTCCCAGCC 
AGTGACACCG GTTCAATGGC AGAGACCCAA GGAGACGAGG AAGAGGAAGA GGGCACTGTA 
GCCAGCTGTA CACGCTGCCT AAATAAGATC GTGAAAACCA GCGTTTGCCG CCGTCTCCGC 
CGAGCCAACC GGGGCCTTCG GGCGAGCTGC CGGAGGGCTG TGAAGTCCAA TGCCTGCTAC 
TGGGCCGTGT TGCTGCTCGT CTTCCTCAAC ACTCTGACAA TCGCCTCGGA GCACCACGGG 
CAGCCCTTCT GGCTCACCCA GATCCAGGAG TATGCCAACA AAGTGCTACT CTGTCTATTC 
ACGGTGGAGA TGCTTCTCAA GTTGTATGGT CTGGGGCCCT CTGTCTACGT GTCCTCCTTC 
TTCAACCGCT TTGACTGCTT CGTGGTCTGT GGGGGCATCC TGGAGACTAC CCTGGTGGAG 
GTGGGTGCCA TGCAGCCCCT GGGCATCTCG GTACTCCGCT GTGTGCGCCT CCTCAGGATC 
TTTAAGGTCA CCAGGCACTG GGCATCTCTG AGCAATTTAG TGGCGTCTCT ACTCAATTCA 
ATGAAATCCA TTGCATCCTT GCTGCTTCTC CTCTTCCTCT TTATCATCAT CTTCTCCCTG 
CTTGGCATGC AGCTGTTTGG GGGCAAGTTC AACTTTGACC AGACCCACAC CAAGCGAAGC 
ACCTTTGACA CCTTTCCCCA GGCCCTCCTC ACTGTCTTTC AGATACTGAC GGGTGAGGAC 
TGGAATGTGG TCATGTATGA TGGTATCATG GCCTATGGCG GCCCCTTCTT CCCAGGGATG 
CTCGTGTGCG TGTATTTCAT CATCCTCTTC ATCTGCGGCA ATTACATCCT ATTGAATGTG 
TTTCTTGCCA TCGCTGTGGA CAACCTGGCC AGTGGAGATG CAGGCACTGA CAAAGATAAG 
GGCAGGGAGA AGATCACTGA GGAAACTCCA CAGGAGAATG GAGCACTGGT GCCTGGTGGG 
GAGAATGAGG AAGAGGAATC TGCAAAAAAT GAAGGAGCAG GCAAGTGGGC AGGCATGGAG 
GAAGAGGAGG AGGAGGAGGA GGAGGAAGAG GAAGTGGGGG CCGGGCATGT GGAACTCCTG 
CAGGAAGTTG TACCCAAGGA GAAGGTGGTA CCCATCCCTG AGGGCAGTGC TTTCTTCTGC 
CTCAGCCAAA CCAACCCGCT GAGGAAGGCC TGCCACACCC TCATCCACCA TCACATCTTC 
ACCAATCTCA TCCTGGTGTT TATCATCCTC AGCAGTGTGT CCCTGGCCGC TGAGGATCCC 
ATCCGAGCCC ACTCCTTCCG CAACCACATT CTGGGGTACT TTGATTATGC CTTCACCTCC 
ATTTTCACTG TGGAGATTCT ACTAAAGATG ACAGTGTTCG GGGCTTTCCT GCACCGTGGT 
TCCTTCTGCC GTAGCTGGTT CAATCTGCTG GATCTGCTGG TGGTCAGCGT GTCCCTCATC 
TCCTTTGGTA TCCACTCCAG CGCCATCTCA GTGGTGAAGA TTCTGCGTGT GCTCCGAGTA 
CTGCGGCCCC TTCGAGCTAT CAACAGGGCC AAAGGACTCA AGCATGTGGT GCAATGTGTG 
TTCGTGGCCA TCCGGACCAT CGGAAATATC ATGATCGTGA CCACGCTCCT GCAGTTCATG 
TTCGCCTGCA TTGGTGTGCA GCTCTTCAAG GGGAAATTCT ACAGTTGCAC TGATGAGGCC 
AAACACACAC CCCAAGAATG CAAGGGCTCC TTCCTGGTCT ACCCCGATGG AGATGTGTCA 
AGGCCTCTGG TCCGGGAGCG GCTCTGGGTC AACAGCGATT TCAACTTTGA CAATGTCCTT 
TCAGCCATGA TGGCCCTGTT CACTGTCTCC ACCTTCGAAG GCTGGCCTGC GCTGCTATAC 
AAGGCCATTG ATGCAAACGC AGAGGACAAG GGCCCCATCT ACAATTACCA CGTGGAGATC 
TCAGTGTTCT TCATTGTCTA TATCATCATC ATTGCATTCT TCATGATGAA CATCTTTGTG 
GGCTTTGTCA TCATCACCTT CCGAGCCCAG GGCGAGCAGG AGTACCAAGA CTGTGAGCTG 
GATAAGAACC AGCGCCAGTG TGTAGAATAC GCCCTCAAGG CCCAACCACT CCGTCGCTAT 
ATCCCCAAGA ACCCACATCA GTATCGTGTG TGGGCCACTG TGAACTCTGC TGCCTTCGAG 
TATCTCATGT TCCTGCTCAT CCTGCTCAAC ACGGTTGCTC TAGCCATGCA GCACTATGAG 
CAGACTGCTC CCTTCAACTA TGCCATGGAC ATCCTCAACA TGGTCTTCAC TGGCCTCTTC 
ACTGTCGAGA TGGTGCTCAA AATCATTGCC TTCAAACCCA AGCATTACTT TACTGATGCC 
TGGAACACAT TTGATGCTCT TATTGTGGTG GGCAGCGTAG TGGACATTGC CGTCACTGAA 
GTCAACAATG GTGGCCACCT GGGCGAGAGC TCTGAGGACA GTTCCCGCAT TTCGATCACC 
TTCTTTCGCC TCTTCCGAGT CATGCGATTG GTCAAGCTTC TTAGTAAGGG TGAAGGGATT 
CGCACATTAC TTTGGACATT CATCAAGTCT TTTCAGGCCT TACCCTATGT GGCTCTTCTC 
ATCGCAATGA TATTCTTCAT TTACGCAGTC ATTGGGATGC AGATGTTTGG CAAGGTGGCT 
CTTCAGGATG GCACACAGAT CAACCGAAAC AACAACTTCC AGACCTTTCC ACAGGCTGTA 
CTGCTTTTGT TCAGGTGTGC TACTGGTGAG GCATGGCAGG AGATAATGCT TGCCAGCCTT 
CCTGGAAGCC GGTGTGACCC TGAGTCTGAC TTCGGCCCTG GTGAGGAGTT TAGCTGTGGT 
AGCAATTTTG CCATCGCCTA TTTTATCAGC TTCTTCATGC TCTGTGCATT CCTGATCATA 
AATCTCTTTG TGGCTGTGAT CATGGACAAC TTTGATTATC TAACCAGAGA TTGGTCCATC 
CTGGGCCCCC ATCACCTCGA TGAATTCAAG AGGATTTGGT CTGAATATGA CCCTGGGGCC 
AAAGGCCGCA TCAAGCACCT GGATGTGGTT GCCCTGCTGA GACGCATCCA GCCCCCACTG 
GGATTTGGGA AGCTGTGCCC ACACCGAGTG GCCTGCAAGA GACTTGTGGC GATGAACATG 
CCCCTCAACT CAGATGGGAC AGTGACATTC AACGCCACAC TATTTGCCCT GGTCCGGACA 
TCACTGAAGA TCAAGACGGA AGGAAACCTG GAGCAAGCCA ATCAGGAGCT GCGGATTGTC 
ATCAAAAAGA TCTGGAAACG GATGAAGCAG AAGCTGCTAG ATGAGGTCAT CCCACCAGCT 
GATGAGGAAG AAGTTACTGT GGGCAAATTC TACGCCACAT TTCTGATCCA GGACTACTTC 
CGCAAATTCC GGCGGAGGAA AGAAAAGGGG CTACTAGGCA CCGAGGCCCC CCCAAGCACC 
TCCTCTGCCC TTCAGGCTGG CCTGAGGAGC CTGCAAGACT TGGGTCCGGA GATGCGGCAG 
GCCCTCACTT GTGACACAGA GGAAGAGGAA GGGGAAGAGG GGCATGAGGG AGAGGAGGAA 
CATGAGGACC TGGAAACATA CACAGCCCTG ATGGGCTCCC AGCCCCCATC TCGCCGGAGC 
TCCGTGATTT CTGTGTCTCT GCCTGCTGGA GACAGACCTC CAGATTCACT TTCCCCTGGA 
CCCAGTGATG ACGATGGGGG GGCTCCCAAC TCCAGACAGT CCAATGTTCC CCAGTCTGGG 
TCCCATGCCC ACAGGAGAAG CTCTGGGGTT CTCATTTTCA CCATTCCAGA AGAAGGAAGT 
TCTCAGTCCA AGGCAACCAA AGGGCAAGAG AGGCAGGATG AGCAAGAGGA AGTCCTGAGC 
CAGCACTCTG GCCACGACAC GCTCTCCTAC CTAGATGAAC AGGCAGGGGC TCCCCCACGC 
CCCATCCTTT TGCCATCTCA CAGACCCCAG AGATACGTGG ATGGCCACCA AGCACCACGC 
CGCCGTCTGC TGCCCCCAAC GCCTGCAGGT CGGAAGCCAT CCTTCAACAT CCAGTGTCTG 
CGGCGCCAGG GCAGCTGTGA AGATTTACCC ATTCCAGGCA CCTATCATCG TGGACGAAAC 
TCAGGGCCCA GTAGGGCACA GGTGCTCATC TCTGAGGGCC TGGGCCTCTT TGCTCGAGAC 
CCACGCTTCG TGGCCCTGGC CAAGCAGGAG ATTGCAGATG CATGTCGCCT GACCCTGGAC 
GAGATGGACA GTGCTGCCAG TGACCTGCTG GCACAGGGGA CCAGCTCACT GTATAGTGAC 
GAAGAGTCCA TCCTCTCCCG TTTTGATGAG GAGGACCTAG GAGACGAGAT GGCCTGCGTC 
CATGCCCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024843622.1
CDS	1..5712
Translation	MSASEGGKDTTPEPSPANGTGPGPEWGLCPGPPASDGEISGASGLGTPKRKTQHSKHKTVAVASAQRSPRALFCLTLANPLRRPFDILILLTIFANCVALGVYIPFPEDDSNTANHNLEQVEYVFLVIFTVETVLKIVAYGLVLHPSAYIRNGWNLLDFIIVVVGLFSVLLEQGPGRPGDTPHMGGKPGGFDVKALRAFRVLRPLRLVSGVPSLHIVLNSIMKALVPLLHIALLVLFVIIIYAIIGLELFLGRMHKTCYFLGSDVEAEEDPSPCASSGSGRACTLNQTECRGRWAGPNGGITNFDNFFFAMLTVFQCITMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLRGYLDWITQAEELDMEDPSAAGNFGSVAEEGLAGHRPQLEELTNRRRGRLRWFSHSTHSTSSHASLPASDTGSMAETQGDEEEEEGTVASCTRCLNKIVKTSVCRRLRRANRGLRASCRRAVKSNACYWAVLLLVFLNTLTIASEHHGQPFWLTQIQEYANKVLLCLFTVEMLLKLYGLGPSVYVSSFFNRFDCFVVCGGILETTLVEVGAMQPLGISVLRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDQTHTKRSTFDTFPQALLTVFQILTGEDWNVVMYDGIMAYGGPFFPGMLVCVYFIILFICGNYILLNVFLAIAVDNLASGDAGTDKDKGREKITEETPQENGALVPGGENEEEESAKNEGAGKWAGMEEEEEEEEEEEEVGAGHVELLQEVVPKEKVVPIPEGSAFFCLSQTNPLRKACHTLIHHHIFTNLILVFIILSSVSLAAEDPIRAHSFRNHILGYFDYAFTSIFTVEILLKMTVFGAFLHRGSFCRSWFNLLDLLVVSVSLISFGIHSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYSCTDEAKHTPQECKGSFLVYPDGDVSRPLVRERLWVNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDANAEDKGPIYNYHVEISVFFIVYIIIIAFFMMNIFVGFVIITFRAQGEQEYQDCELDKNQRQCVEYALKAQPLRRYIPKNPHQYRVWATVNSAAFEYLMFLLILLNTVALAMQHYEQTAPFNYAMDILNMVFTGLFTVEMVLKIIAFKPKHYFTDAWNTFDALIVVGSVVDIAVTEVNNGGHLGESSEDSSRISITFFRLFRVMRLVKLLSKGEGIRTLLWTFIKSFQALPYVALLIAMIFFIYAVIGMQMFGKVALQDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLASLPGSRCDPESDFGPGEEFSCGSNFAIAYFISFFMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPGAKGRIKHLDVVALLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVTFNATLFALVRTSLKIKTEGNLEQANQELRIVIKKIWKRMKQKLLDEVIPPADEEEVTVGKFYATFLIQDYFRKFRRRKEKGLLGTEAPPSTSSALQAGLRSLQDLGPEMRQALTCDTEEEEGEEGHEGEEEHEDLETYTALMGSQPPSRRSSVISVSLPAGDRPPDSLSPGPSDDDGGAPNSRQSNVPQSGSHAHRRSSGVLIFTIPEEGSSQSKATKGQERQDEQEEVLSQHSGHDTLSYLDEQAGAPPRPILLPSHRPQRYVDGHQAPRRRLLPPTPAGRKPSFNIQCLRRQGSCEDLPIPGTYHRGRNSGPSRAQVLISEGLGLFARDPRFVALAKQEIADACRLTLDEMDSAASDLLAQGTSSLYSDEESILSRFDEEDLGDEMACVHAL

Target ORF information:

RefSeq Version	XM_024987854.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 F (CACNA1F), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024987854.1

ORF Insert Sequence:

ATGTCGGCAT CTGAAGGCGG GAAAGACACC ACCCCAGAGC CCAGTCCAGC CAATGGGACA 
GGCCCTGGGC CCGAATGGGG GCTGTGCCCT GGGCCCCCAG CATCAGATGG TGAAATCAGC 
GGGGCATCGG GCCTAGGGAC CCCTAAGCGA AAGACTCAGC ACAGTAAGCA CAAGACAGTG 
GCAGTAGCCA GTGCCCAGCG GTCACCCCGG GCACTCTTCT GCCTCACCCT GGCAAACCCC 
CTGCGGCGGC CCTTCGACAT CCTCATCTTG CTGACCATCT TTGCCAATTG CGTGGCCCTG 
GGAGTCTACA TCCCCTTCCC GGAGGACGAC TCCAACACTG CCAACCACAA CCTGGAGCAA 
GTGGAATACG TATTTTTGGT GATTTTCACT GTGGAGACGG TGCTCAAGAT CGTGGCCTAT 
GGACTGGTGC TCCACCCCAG CGCCTACATC CGCAACGGCT GGAACCTGCT CGACTTCATC 
ATTGTCGTGG TCGGGCTGTT CAGCGTGCTG CTGGAGCAGG GTCCCGGACG CCCAGGGGAC 
ACCCCGCATA TGGGAGGGAA GCCAGGGGGC TTCGATGTGA AGGCATTGCG GGCATTTCGG 
GTGCTGCGGC CACTGAGGCT GGTGTCTGGG GTCCCAAGCC TGCACATAGT GCTCAATTCC 
ATCATGAAGG CTCTGGTGCC GCTGTTGCAT ATTGCACTTC TTGTGCTCTT TGTCATCATC 
ATTTACGCCA TCATTGGACT GGAGCTGTTC CTCGGACGCA TGCACAAAAC GTGCTACTTC 
CTGGGATCTG ACGTAGAGGC GGAGGAGGAC CCGTCACCCT GTGCGTCCTC AGGATCAGGG 
CGTGCTTGCA CACTGAACCA GACGGAGTGC CGCGGGCGCT GGGCAGGGCC CAACGGAGGC 
ATCACCAACT TTGACAACTT TTTCTTCGCC ATGCTGACAG TCTTCCAGTG CATCACCATG 
GAAGGCTGGA CCGACGTGCT CTACTGGATG CAGGATGCCA TGGGGTATGA GCTGCCCTGG 
GTGTATTTTG TGAGTCTCGT CATCTTTGGA TCCTTCTTCG TCCTCAACCT TGTGCTTGGT 
GTCCTGAGTG GGGAGTTCTC CAAAGAGAGG GAGAAGGCAA AAGCACGAGG GGATTTCCAG 
AAGCTTCGGG AGAAGCAGCA GCTGGAGGAG GACCTCCGGG GCTACCTGGA CTGGATCACA 
CAGGCGGAGG AGCTGGACAT GGAGGACCCC TCAGCTGCTG GCAACTTTGG TTCCGTGGCT 
GAAGAGGGCC TGGCCGGCCA CCGGCCACAA CTGGAAGAGC TGACCAATAG GAGACGAGGA 
CGTCTACGCT GGTTCAGTCA CTCCACCCAC TCCACCAGCA GCCATGCCAG CCTCCCAGCC 
AGTGACACCG GTTCAATGGC AGAGACCCAA GGAGACGAGG AAGAGGAAGA GGGCACTGTA 
GCCAGCTGTA CACGCTGCCT AAATAAGATC GTGAAAACCA GCGTTTGCCG CCGTCTCCGC 
CGAGCCAACC GGGGCCTTCG GGCGAGCTGC CGGAGGGCTG TGAAGTCCAA TGCCTGCTAC 
TGGGCCGTGT TGCTGCTCGT CTTCCTCAAC ACTCTGACAA TCGCCTCGGA GCACCACGGG 
CAGCCCTTCT GGCTCACCCA GATCCAGGAG TATGCCAACA AAGTGCTACT CTGTCTATTC 
ACGGTGGAGA TGCTTCTCAA GTTGTATGGT CTGGGGCCCT CTGTCTACGT GTCCTCCTTC 
TTCAACCGCT TTGACTGCTT CGTGGTCTGT GGGGGCATCC TGGAGACTAC CCTGGTGGAG 
GTGGGTGCCA TGCAGCCCCT GGGCATCTCG GTACTCCGCT GTGTGCGCCT CCTCAGGATC 
TTTAAGGTCA CCAGGCACTG GGCATCTCTG AGCAATTTAG TGGCGTCTCT ACTCAATTCA 
ATGAAATCCA TTGCATCCTT GCTGCTTCTC CTCTTCCTCT TTATCATCAT CTTCTCCCTG 
CTTGGCATGC AGCTGTTTGG GGGCAAGTTC AACTTTGACC AGACCCACAC CAAGCGAAGC 
ACCTTTGACA CCTTTCCCCA GGCCCTCCTC ACTGTCTTTC AGATACTGAC GGGTGAGGAC 
TGGAATGTGG TCATGTATGA TGGTATCATG GCCTATGGCG GCCCCTTCTT CCCAGGGATG 
CTCGTGTGCG TGTATTTCAT CATCCTCTTC ATCTGCGGCA ATTACATCCT ATTGAATGTG 
TTTCTTGCCA TCGCTGTGGA CAACCTGGCC AGTGGAGATG CAGGCACTGA CAAAGATAAG 
GGCAGGGAGA AGATCACTGA GGAAACTCCA CAGGAGAATG GAGCACTGGT GCCTGGTGGG 
GAGAATGAGG AAGAGGAATC TGCAAAAAAT GAAGGAGCAG GCAAGTGGGC AGGCATGGAG 
GAAGAGGAGG AGGAGGAGGA GGAGGAAGAG GAAGTGGGGG CCGGGCATGT GGAACTCCTG 
CAGGAAGTTG TACCCAAGGA GAAGGTGGTA CCCATCCCTG AGGGCAGTGC TTTCTTCTGC 
CTCAGCCAAA CCAACCCGCT GAGGAAGGCC TGCCACACCC TCATCCACCA TCACATCTTC 
ACCAATCTCA TCCTGGTGTT TATCATCCTC AGCAGTGTGT CCCTGGCCGC TGAGGATCCC 
ATCCGAGCCC ACTCCTTCCG CAACCACATT CTGGGGTACT TTGATTATGC CTTCACCTCC 
ATTTTCACTG TGGAGATTCT ACTAAAGATG ACAGTGTTCG GGGCTTTCCT GCACCGTGGT 
TCCTTCTGCC GTAGCTGGTT CAATCTGCTG GATCTGCTGG TGGTCAGCGT GTCCCTCATC 
TCCTTTGGTA TCCACTCCAG CGCCATCTCA GTGGTGAAGA TTCTGCGTGT GCTCCGAGTA 
CTGCGGCCCC TTCGAGCTAT CAACAGGGCC AAAGGACTCA AGCATGTGGT GCAATGTGTG 
TTCGTGGCCA TCCGGACCAT CGGAAATATC ATGATCGTGA CCACGCTCCT GCAGTTCATG 
TTCGCCTGCA TTGGTGTGCA GCTCTTCAAG GGGAAATTCT ACAGTTGCAC TGATGAGGCC 
AAACACACAC CCCAAGAATG CAAGGGCTCC TTCCTGGTCT ACCCCGATGG AGATGTGTCA 
AGGCCTCTGG TCCGGGAGCG GCTCTGGGTC AACAGCGATT TCAACTTTGA CAATGTCCTT 
TCAGCCATGA TGGCCCTGTT CACTGTCTCC ACCTTCGAAG GCTGGCCTGC GCTGCTATAC 
AAGGCCATTG ATGCAAACGC AGAGGACAAG GGCCCCATCT ACAATTACCA CGTGGAGATC 
TCAGTGTTCT TCATTGTCTA TATCATCATC ATTGCATTCT TCATGATGAA CATCTTTGTG 
GGCTTTGTCA TCATCACCTT CCGAGCCCAG GGCGAGCAGG AGTACCAAGA CTGTGAGCTG 
GATAAGAACC AGCGCCAGTG TGTAGAATAC GCCCTCAAGG CCCAACCACT CCGTCGCTAT 
ATCCCCAAGA ACCCACATCA GTATCGTGTG TGGGCCACTG TGAACTCTGC TGCCTTCGAG 
TATCTCATGT TCCTGCTCAT CCTGCTCAAC ACGGTTGCTC TAGCCATGCA GCACTATGAG 
CAGACTGCTC CCTTCAACTA TGCCATGGAC ATCCTCAACA TGGTCTTCAC TGGCCTCTTC 
ACTGTCGAGA TGGTGCTCAA AATCATTGCC TTCAAACCCA AGCATTACTT TACTGATGCC 
TGGAACACAT TTGATGCTCT TATTGTGGTG GGCAGCGTAG TGGACATTGC CGTCACTGAA 
GTCAACAATG GTGGCCACCT GGGCGAGAGC TCTGAGGACA GTTCCCGCAT TTCGATCACC 
TTCTTTCGCC TCTTCCGAGT CATGCGATTG GTCAAGCTTC TTAGTAAGGG TGAAGGGATT 
CGCACATTAC TTTGGACATT CATCAAGTCT TTTCAGGCCT TACCCTATGT GGCTCTTCTC 
ATCGCAATGA TATTCTTCAT TTACGCAGTC ATTGGGATGC AGATGTTTGG CAAGGTGGCT 
CTTCAGGATG GCACACAGAT CAACCGAAAC AACAACTTCC AGACCTTTCC ACAGGCTGTA 
CTGCTTTTGT TCAGGTGTGC TACTGGTGAG GCATGGCAGG AGATAATGCT TGCCAGCCTT 
CCTGGAAGCC GGTGTGACCC TGAGTCTGAC TTCGGCCCTG GTGAGGAGTT TAGCTGTGGT 
AGCAATTTTG CCATCGCCTA TTTTATCAGC TTCTTCATGC TCTGTGCATT CCTGATCATA 
AATCTCTTTG TGGCTGTGAT CATGGACAAC TTTGATTATC TAACCAGAGA TTGGTCCATC 
CTGGGCCCCC ATCACCTCGA TGAATTCAAG AGGATTTGGT CTGAATATGA CCCTGGGGCC 
AAAGGCCGCA TCAAGCACCT GGATGTGGTT GCCCTGCTGA GACGCATCCA GCCCCCACTG 
GGATTTGGGA AGCTGTGCCC ACACCGAGTG GCCTGCAAGA GACTTGTGGC GATGAACATG 
CCCCTCAACT CAGATGGGAC AGTGACATTC AACGCCACAC TATTTGCCCT GGTCCGGACA 
TCACTGAAGA TCAAGACGGA AGGAAACCTG GAGCAAGCCA ATCAGGAGCT GCGGATTGTC 
ATCAAAAAGA TCTGGAAACG GATGAAGCAG AAGCTGCTAG ATGAGGTCAT CCCACCAGCT 
GATGAGGAAG AAGTTACTGT GGGCAAATTC TACGCCACAT TTCTGATCCA GGACTACTTC 
CGCAAATTCC GGCGGAGGAA AGAAAAGGGG CTACTAGGCA CCGAGGCCCC CCCAAGCACC 
TCCTCTGCCC TTCAGGCTGG CCTGAGGAGC CTGCAAGACT TGGGTCCGGA GATGCGGCAG 
GCCCTCACTT GTGACACAGA GGAAGAGGAA GGGGAAGAGG GGCATGAGGG AGAGGAGGAA 
CATGAGGACC TGGAAACATA CACAGCCCTG ATGGGCTCCC AGCCCCCATC TCGCCGGAGC 
TCCGTGATTT CTGTGTCTCT GCCTGCTGGA GACAGACCTC CAGATTCACT TTCCCCTGGA 
CCCAGTGATG ACGATGGGGG GGCTCCCAAC TCCAGACAGT CCAATGTTCC CCAGTCTGGG 
TCCCATGCCC ACAGGAGAAG CTCTGGGGTT CTCATTTTCA CCATTCCAGA AGAAGGAAGT 
TCTCAGTCCA AGGCAACCAA AGGGCAAGAG AGGCAGGATG AGCAAGAGGA AGTCCTGAGC 
CAGCACTCTG GCCACGACAC GCTCTCCTAC CTAGATGAAC AGGCAGGGGC TCCCCCACGC 
CCCATCCTTT TGCCATCTCA CAGACCCCAG AGATACGTGG ATGGCCACCA AGCACCACGC 
CGCCGTCTGC TGCCCCCAAC GCCTGCAGGT CGGAAGCCAT CCTTCAACAT CCAGTGTCTG 
CGGCGCCAGG GCAGCTGTGA AGATTTACCC ATTCCAGGCA CCTATCATCG TGGACGAAAC 
TCAGGGCCCA GTAGGGCACA GGTGCTCATC TCTGAGGGCC TGGGCCTCTT TGCTCGAGAC 
CCACGCTTCG TGGCCCTGGC CAAGCAGGAG ATTGCAGATG CATGTCGCCT GACCCTGGAC 
GAGATGGACA GTGCTGCCAG TGACCTGCTG GCACAGGGGA CCAGCTCACT GTATAGTGAC 
GAAGAGTCCA TCCTCTCCCG TTTTGATGAG GAGGACCTAG GAGACGAGAT GGCCTGCGTC 
CATGCCCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Marçais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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CACNA1F cDNA ORF clone, Bos taurus(cattle)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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