CACNA1F cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	CACNA1F
Entrez Gene ID	480915
Full Name	calcium voltage-gated channel subunit alpha1 F
General protein information	Preferred Names calcium voltage-gated channel subunit alpha1 F Names voltage-dependent L-type calcium channel subunit alpha-1F LOW QUALITY PROTEIN: voltage-dependent L-type calcium channel subunit alpha-1F
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005642089.2	XP_005642146.1	LOW QUALITY PROTEIN: voltage-dependent L-type calcium channel subunit alpha-1F
XM_005642089.3	XP_005642146.2	LOW QUALITY PROTEIN: voltage-dependent L-type calcium channel subunit alpha-1F

Pathways from BioSystems

Homologies

Homo sapiens (human)	CACNA1F	NP_005174.2
Macaca mulatta (Rhesus monkey)	CACNA1F	XP_001106163.2
Canis lupus familiaris (dog)	CACNA1F	XP_005642146.1
Danio rerio (zebrafish)	LOC559964	XP_003199134.2
Bos taurus (cattle)	CACNA1F	NP_001192592.1
Mus musculus (house mouse)	Cacna1f	NP_062528.2
Danio rerio (zebrafish)	LOC793708	XP_001333514.5
Xenopus tropicalis (tropical clawed frog)	cacna1f	XP_002941018.2
Rattus norvegicus (Norway rat)	Cacna1f	NP_446153.1

Gene Ontology

The following CACNA1F gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CACNA1F cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe285495	XM_005642089.3 Latest version!	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 F (CACNA1F), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCe33235	XM_005642089.2 Latest version!	Canis lupus familiaris calcium channel, voltage-dependent, L type, alpha 1F subunit (CACNA1F), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CACNA1F ( XM_005642089.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005642146.2 Canis lupus familiaris calcium voltage-gated channel subunit alpha1 F (CACNA1F), mRNA.

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CloneID	OCe285495
Clone ID Related Accession (Same CDS sequence)	XM_005642089.3
Accession Version	XM_005642089.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	LOW QUALITY PROTEIN: voltage-dependent L-type calcium channel subunit alpha-1F
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005642089.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGTGCAGAT GGCTCTTCAA CCTCGAAAGG AGGATGTCGG CATCTGATGG CGGGAAAGAC 
ACCACCCCAG AGCCCAGCCC AGTCAATGGG ACAGGCCCTG GGCCTGAATG GGGGCTGTGT 
CCTGGGCCTC CAGCATCAGG GGGTGAAGAA ATCAGCGGGG CCGTGGGCCC AGAGACCCCT 
AAGCGAAGAA CTCAGCACAA TAAGCACAAG ACGGTGGCAG TGGCCAGTGC CCAACGGTCA 
CCCCGGGCGC TCTTCTGCCT CACTCTGGCC AACCCGCTGC GGCGGTCCTG CATCAGCATT 
GTGGAGTGGA AGCCCTTTGA CATCCTCATC CTGCTGACCA TCTTTGCCAA CTGTGTAGCC 
CTGGGGGTCT ACATCCCCTT CCCAGAGGAT GACTCCAACA CTGCTAACCA CAACCTGGAG 
CAAGTGGAGT ATGTATTCCT GGTGATTTTC ACTGTGGAAA CCGTGCTCAA GATCGTGGCC 
TATGGGCTGG TGCTCCATCC CAGCGCCTAC ATCCGCAACG GCTGGAACCT ACTTGACTTC 
ATCATCGTTG TGGTCGGGCT GTTCAGCGTG CTACTTGAGC AGGGCCCTGG TCGGCCGGGG 
GACACCCCGC ATACCGGGGG GAAGCCGGGG GGCTTCGATG TGAAGGCGTT GAGGGCGTTT 
CGGGTGCTGC GGCCACTGAG GCTGGTATCT GGGGTGCCAA GCCTGCACAT AGTGCTCAAT 
TCCATCATGA AGGCGCTGGT GCCGCTACTG CACATTGCAC TGCTCGTGCT CTTCGTCATC 
ATCATCTATG CCATCATCGG ACTCGAGCTG TTCCTTGGAC GCATGCACAA GACATGTTAC 
TTCCTGGGAT CTGACCTGGA AGCGGAGGAG GACCCGTCGC CCTGTGCGTC TTCGGGCNTC 
GGGCGAGCGT GCACGCTGAA CCAGACCGAG TGCCGGGGGC GCTGGGCAGG GCCCAACGGA 
GGCATCACCA ACTTCGACAA CTTCTTCTTC GCCATGCTCA CGGTCTTCCA GTGCGTCACC 
ATGGAAGGCT GGACCGACGT GCTCTACTGG ATGCAGGACG CCATGGGGTA TGAGCTGCCC 
TGGGTATATT TCGTGAGTCT CGTCATCTTC GGGTCCTTCT TCGTCCTCAA CCTTGTGCTT 
GGTGTCCTGA GTGGGGAGTT CTCCAAGGAG AGGGAGAAGG CCAAAGCACG AGGGGATTTC 
CAGAAGCTGC GGGAGAAGCA GCAGTTGGAG GAGGACCTAC GGGGCTACCT GGACTGGATC 
ACGCAGGCAG AGGAGCTGGA CCTCGAAGAC CCCTCAACCG ATGGCAACTT TGGTTCTATG 
GCTGAAGAGG GCCGGGCCGG CCACCGGCCA CAGCTGGCAG ATCTGACCAC CAGGAGACGT 
GGACATCTGC GCTGGTTCAG TCACTCCACC CGCTCCACAC ACTCCACCAG CAGCCATGCC 
AGCCTCCCAG CCAGTGACAC CGGTTCGATG GCAGAGATGC CAGGCGATGA GGAAGAGGAG 
GAGGGGGCTC TGGCCAGTTG TACACGCTGC CTAAACAAGA TCATGAAAAC CAGGGTCTGC 
CGCCGCCTCC GCCGAGTCAA CCGAGGACTC CGGGCACGCT GCCGGCGGGC TGTGAAGTCC 
ACCGCTTGCT ACTGGGCCGT GCTGCTGCTC GTCTTCCTCA ACACACTGAC CATAGCCTCA 
GAGCACCATG GCCAACCCAT GTGGCTTACC CAGATCCAGG AGTATGCCAA CAAGGTGTTG 
CTCTGTCTGT TCACGGTGGA GATGCTCCTC AAGTTGTACG GTCTGGGTCC TTCTGTCTAT 
GTTTCTTCCT TCTTCAACCG CTTTGACTGC TTCGTGGTCT GTGGGGGCAT CCTGGAGACC 
ACCCTGGTGG AGGTGGGCGC CATGCAGCCC CTGGGCATCT CAGTGCTCCG ATGTGTGCGC 
CTCCTCAGGA TCTTTAAGGT CACTAGGCAC TGGGCATCTC TGAGCAATTT AGTGGCATCC 
CTGCTCAATT CAATGAAATC CATCGCATCG TTGCTGCTTC TCCTCTTCCT CTTCATCATC 
ATCTTCTCCC TGCTTGGCAT GCAGCTGTTT GGGGGCAAGT TCAACTTTGA CCAGACCCAC 
ACCAAGCGGA ACACCTTTGA TACCTTCCCT CAGGCCCTCC TCACTGTCTT TCAGATCCTG 
ACATGTGAGG ATTGGAACGT GGGCATGTAT GATGGCATCA TGGCCTATGG TGGCCCCTTC 
TTCCCAGGAA TGCTGGTGTG TGTCTATTTT ATCATCCTCT TCATCTGTGG CAACTCAATC 
ACTCCCCTTT CCTCGAACAG GGAGAAGAGC ACCGAGGGGG CTCTCCCACA AGAAAATGGA 
GTGTTGGTGG CTGGTGGGGA GAATGAGGAA GAGGAGGGCA GAAAGAATGA AGGAGCAGGC 
AAGTGGCAGG GCCGGGTGGA GAAGGAAGAA GAGGAAGAGG AGGAGGGGGA GGAAGAGGAG 
GAAGGGGGTG CAGGGCATGT GGAACTCCTG CAGGAAGTTG TACCCAAGGA GAAGGTGGTA 
CCCATCCCTG AGGGCAGCGC CTTCTTCTGT CTCAGCCAAA CCAACCCGCT GAGGAAGGCC 
TGCCACACCC TCATCCACCA TCATATCTTC ACCAATCTTA TTCTGGTATT CATCATCCTC 
AGCAGTGTGT CCTTGGCCGC TGAGGACCCC ATCCGAGCCC ACTCCTTCCG CAACCATATT 
CTGGGGTATT TTGATTATGC TTTCACCTCT ATTTTCACTG TGGAGATTCT ACTAAAGATG 
ACAGTATTTG GGGCCTTCCT GCATCGAGGA TCCTTCTGCC GTAGCTGGTT CAATCTATTG 
GATCTGCTGG TGGTCAGCGT ATCCCTCATC TCCTTTGGCA TCCACTCCAG CGCCATCTCA 
GTGGTGAAAA TTCTGAGGGT ACTCCGAGTC CTGAGGCCTC TCCGAGCCAT CAACAGAGCC 
AAGGGACTCA AGCATGTTGT GCAATGTGTG TTTGTGGCTA TCCGGACCAT TGGGAATATC 
ATGATTGTGA CCACACTCCT GCAATTCATG TTTGCCTGCA TTGGCGTGCA GCTCTTCAAG 
GGGAAATTCT ACAGTTGCAC TGATGAAGCC AAACACACCC CACAAGAATG CAAGGGCTCC 
TTCCTGGTCT ACCCTGATGG AGAGGTATCA CGACCCCTGG TCCGGGAGCG GCTCTGGGTC 
AACAGCGATT TCAACTTTGA CAATGTCCTC TCAGCCATGG ATGGCCTTGT TCCTGTCTCT 
ACCTTCGAAG GTTGGCCTGC TCTACTGTAC AAGGCCATCG ATGCAAATGC AGAGGACAAG 
GGCCCTATCT ATAATTACCA TGTGGAGATC TCAGTGTTCT TCATTGTCTA CATCATCATC 
ATTGCATTCT TCATGATGAA TATCTTTGTG GGCTTTGTCA TCATCACCTT CCGTGCCCAG 
GGCGAGCAGG AATACCAAAA CTGTGAGCTG GACAAGAACC AGCGCCAGTG TGTGGAATAT 
GCCCTCAAGG CCCAGCCACT CCGCCGCTAC ATCCCCAAGA ACCCACATCA ATATCGTGTG 
TGGGCCACTG TGAACTCTGC TGCCTTCGAG TACCTCATGT TCCTGCTCAT CCTGCTCAAC 
ACCGTTGCTC TAGCAATGCA GCACTATGAA CAGACTGCTC CCTTTAATTA TGCCATGGAC 
ATCCTCAACA TGGTCTTCAC TGGCCTCTTC ACTATTGAGA TGGTGCTCAA AATCATCGCC 
TTCAAACCCA AGCATTACTT TACCGATGCC TGGAACACGT TTGATGCTCT TATTGTAGTG 
GGCAGCGTAG TGGACATTGC CGTCACTGAA GTCAACAATG GTGGCCACCT TGGCGAGAGC 
TCTGAGGACA GTTCCCGCAT TTCCATCACT TTCTTTCGCC TCTTCCGAGT CATGCGACTA 
GTCAAGCTTC TCAGTAAAGG TGAAGGAATC CGCACATTGC TCTGGACATT CATCAAGTCC 
TTCCAGGCCT TGCCCTATGT GGCTCTTCTC ATCGCAATGA TATTCTTCAT TTATGCAGTC 
ATTGGAATGC AGATGTTTGG CAAGGTGGCT CTTCAGGATG GCACACAGAT CAACCGAAAC 
AACAACTTCC AGACCTTTCC ACAGGCTGTG CTACTTTTGT TCAGGTGTGC CACCGGTGAG 
GCGTGGCAAG AGATAATGCT CGCCAGCCTT CCCGGGAGTC GGTGTGACCC TGAATCTGAC 
GTCAGCCCTG GCGAGGAGTT TACCTGTGGC AGCAATTTTG CCATCGCCTA TTTTATCAGC 
TTCTTCATGC TCTGTGCCTT CCTGATCATA AATCTCTTTG TGGCTGTCAT CATGGATAAC 
TTTGACTATC TAACCAGAGA TTGGTCCATC CTGGGCCCCC ATCACCTCGA TGAATTCAAG 
AGGATCTGGT CTGAATATGA CCCTGGAGCC AAGGGCCGCA TCAAGCACCT GGATGTGGTG 
GCCCTGCTGA GACGCATCCA GCCCCCCTTG GGATTTGGGA AGCTCTGCCC ACACCGAGTA 
GCCTGCAAGA GACTTGTGGC GATGAACATG CCCCTCAACT CAGATGGGAC AGTGACATTC 
AATGCCACAC TCTTTGCCCT GGTTCGGACA TCCTTGAAGA TCAAGACAGA AGGGAACCTG 
GAACAAGCCA ATCAGGAGCT GCGGATTGTC ATCAAAAAGA TCTGGAAGCG GATGAAGCAG 
AAGCTACTAG ATGAGGTCAT CCCCCCTGCT GACGAGGAAG AGGTCACCGT GGGCAAATTC 
TACGCCACAT TTCTGATCCA GGACTATTTC CGCAAATTCC GGCGGAGGAA AGAAAAGGGG 
CTATTAGGGA GCGAGGCCCC CTCCAGTACC TCCTCTGCCC TTCAGGCTGG TCTGAGGAGC 
CTACACGACT TGGGTCCTGA GATCAGGCAG GCCCTCACCT GTGACACAGA TGAGGAGGAG 
AAGGAGGAAG AGGGGCTGGA GGGAGAGGAG GAGGAAGATG AGAAACACCC GGAAACACAC 
AAAGCCCAGA TGGGCTCCCA GCCCCCATCT CGCCGGAGCT CCGTGATTTC TGTGTCTCTG 
CCTGTTGGGG ACAGACTTCC AGATTCACTC TCCCTTGGCC CGAGTGATGA TGATGGGGGG 
GCTTCCAATT CCAGACAGTC CAGTGTACCC CAGGCTGGAT CCCATGGCCA CAGGAGAAGC 
TCTGGGGGTT TCATTTTCAC CATCCCCGAA GAAGGAAGTT CTCAGCACAA GGGAACCAAA 
GAGCAAGAGA ATCAGGAGGA GGAAGAGGAA ATTCCCACCA AAGACTCTGG CCACAACAGG 
CTCTCCTACC TAGATGAGCA GGCAGGGACT CCCCCACGCC CCATCCTTTT GCCACCCCAC 
AGACCCCAGA GATGTGCTGA TGGGCACAAT GCACCACGCC GCCGTCTACT GCCCCCCACG 
CCTGCAGAGC GGAAGCCTTC CTTCACCATA CAGTGTCTGC GGCGCCAGGG CAGTTGTGAG 
GATTTACCCA TCCCAGGCAC CTATCATCGT GGGCGCAACT CAGGGCCCAG CAGGGCTCAG 
GGTTCTTGGG CAACCCCTCC TCAACGGGGT CGGCTCCTGT ATGCCCCACT GTTATTGGTG 
GAGGAGGGCG CAGCAGGGGA GGGATACCTC GGCAAGTCCA GCGGTCCGCT GCGTACCTTC 
ACCTGTCTGC GTGTGCCCGG AACCCACTCA GACTCCAGCC ATGGCAAGAG GGGCAGTGCT 
GACAGTCTGG TGGAGGCTGT GCTCATCTCC GAGGGCTTAG GCCTCTTTGC CCGAGACCCA 
CGCTTTGTGG CCCTAGCCAA GCAGGAGATT GCAGATGCAT GCCGCCTGAC ACTGGACGAG 
ATGGACAGTG CTGCCAGTGA CCTGTTGGCA CAGGGGACCA GCTCACTGTA TAGTGATGAG 
GAATCTATCC TCTCTCGCTT TGATGAGGAG GACCTGGGAG ATGAAATGGC CTGTGTCCAT 
GCCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005642146.2
CDS	39..5987
Translation	MCRWLFNLERRMSASDGGKDTTPEPSPVNGTGPGPEWGLCPGPPASGGEEISGAVGPETPKRRTQHNKHKTVAVASAQRSPRALFCLTLANPLRRSCISIVEWKPFDILILLTIFANCVALGVYIPFPEDDSNTANHNLEQVEYVFLVIFTVETVLKIVAYGLVLHPSAYIRNGWNLLDFIIVVVGLFSVLLEQGPGRPGDTPHTGGKPGGFDVKALRAFRVLRPLRLVSGVPSLHIVLNSIMKALVPLLHIALLVLFVIIIYAIIGLELFLGRMHKTCYFLGSDLEAEEDPSPCASSGnullGRACTLNQTECRGRWAGPNGGITNFDNFFFAMLTVFQCVTMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLRGYLDWITQAEELDLEDPSTDGNFGSMAEEGRAGHRPQLADLTTRRRGHLRWFSHSTRSTHSTSSHASLPASDTGSMAEMPGDEEEEEGALASCTRCLNKIMKTRVCRRLRRVNRGLRARCRRAVKSTACYWAVLLLVFLNTLTIASEHHGQPMWLTQIQEYANKVLLCLFTVEMLLKLYGLGPSVYVSSFFNRFDCFVVCGGILETTLVEVGAMQPLGISVLRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDQTHTKRNTFDTFPQALLTVFQILTCEDWNVGMYDGIMAYGGPFFPGMLVCVYFIILFICGNSITPLSSNREKSTEGALPQENGVLVAGGENEEEEGRKNEGAGKWQGRVEKEEEEEEEGEEEEEGGAGHVELLQEVVPKEKVVPIPEGSAFFCLSQTNPLRKACHTLIHHHIFTNLILVFIILSSVSLAAEDPIRAHSFRNHILGYFDYAFTSIFTVEILLKMTVFGAFLHRGSFCRSWFNLLDLLVVSVSLISFGIHSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYSCTDEAKHTPQECKGSFLVYPDGEVSRPLVRERLWVNSDFNFDNVLSAMDGLVPVSTFEGWPALLYKAIDANAEDKGPIYNYHVEISVFFIVYIIIIAFFMMNIFVGFVIITFRAQGEQEYQNCELDKNQRQCVEYALKAQPLRRYIPKNPHQYRVWATVNSAAFEYLMFLLILLNTVALAMQHYEQTAPFNYAMDILNMVFTGLFTIEMVLKIIAFKPKHYFTDAWNTFDALIVVGSVVDIAVTEVNNGGHLGESSEDSSRISITFFRLFRVMRLVKLLSKGEGIRTLLWTFIKSFQALPYVALLIAMIFFIYAVIGMQMFGKVALQDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLASLPGSRCDPESDVSPGEEFTCGSNFAIAYFISFFMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPGAKGRIKHLDVVALLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVTFNATLFALVRTSLKIKTEGNLEQANQELRIVIKKIWKRMKQKLLDEVIPPADEEEVTVGKFYATFLIQDYFRKFRRRKEKGLLGSEAPSSTSSALQAGLRSLHDLGPEIRQALTCDTDEEEKEEEGLEGEEEEDEKHPETHKAQMGSQPPSRRSSVISVSLPVGDRLPDSLSLGPSDDDGGASNSRQSSVPQAGSHGHRRSSGGFIFTIPEEGSSQHKGTKEQENQEEEEEIPTKDSGHNRLSYLDEQAGTPPRPILLPPHRPQRCADGHNAPRRRLLPPTPAERKPSFTIQCLRRQGSCEDLPIPGTYHRGRNSGPSRAQGSWATPPQRGRLLYAPLLLVEEGAAGEGYLGKSSGPLRTFTCLRVPGTHSDSSHGKRGSADSLVEAVLISEGLGLFARDPRFVALAKQEIADACRLTLDEMDSAASDLLAQGTSSLYSDEESILSRFDEEDLGDEMACVHAL

Target ORF information:

RefSeq Version	XM_005642089.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris calcium voltage-gated channel subunit alpha1 F (CACNA1F), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005642089.3

ORF Insert Sequence:

ATGTGCAGAT GGCTCTTCAA CCTCGAAAGG AGGATGTCGG CATCTGATGG CGGGAAAGAC 
ACCACCCCAG AGCCCAGCCC AGTCAATGGG ACAGGCCCTG GGCCTGAATG GGGGCTGTGT 
CCTGGGCCTC CAGCATCAGG GGGTGAAGAA ATCAGCGGGG CCGTGGGCCC AGAGACCCCT 
AAGCGAAGAA CTCAGCACAA TAAGCACAAG ACGGTGGCAG TGGCCAGTGC CCAACGGTCA 
CCCCGGGCGC TCTTCTGCCT CACTCTGGCC AACCCGCTGC GGCGGTCCTG CATCAGCATT 
GTGGAGTGGA AGCCCTTTGA CATCCTCATC CTGCTGACCA TCTTTGCCAA CTGTGTAGCC 
CTGGGGGTCT ACATCCCCTT CCCAGAGGAT GACTCCAACA CTGCTAACCA CAACCTGGAG 
CAAGTGGAGT ATGTATTCCT GGTGATTTTC ACTGTGGAAA CCGTGCTCAA GATCGTGGCC 
TATGGGCTGG TGCTCCATCC CAGCGCCTAC ATCCGCAACG GCTGGAACCT ACTTGACTTC 
ATCATCGTTG TGGTCGGGCT GTTCAGCGTG CTACTTGAGC AGGGCCCTGG TCGGCCGGGG 
GACACCCCGC ATACCGGGGG GAAGCCGGGG GGCTTCGATG TGAAGGCGTT GAGGGCGTTT 
CGGGTGCTGC GGCCACTGAG GCTGGTATCT GGGGTGCCAA GCCTGCACAT AGTGCTCAAT 
TCCATCATGA AGGCGCTGGT GCCGCTACTG CACATTGCAC TGCTCGTGCT CTTCGTCATC 
ATCATCTATG CCATCATCGG ACTCGAGCTG TTCCTTGGAC GCATGCACAA GACATGTTAC 
TTCCTGGGAT CTGACCTGGA AGCGGAGGAG GACCCGTCGC CCTGTGCGTC TTCGGGCNTC 
GGGCGAGCGT GCACGCTGAA CCAGACCGAG TGCCGGGGGC GCTGGGCAGG GCCCAACGGA 
GGCATCACCA ACTTCGACAA CTTCTTCTTC GCCATGCTCA CGGTCTTCCA GTGCGTCACC 
ATGGAAGGCT GGACCGACGT GCTCTACTGG ATGCAGGACG CCATGGGGTA TGAGCTGCCC 
TGGGTATATT TCGTGAGTCT CGTCATCTTC GGGTCCTTCT TCGTCCTCAA CCTTGTGCTT 
GGTGTCCTGA GTGGGGAGTT CTCCAAGGAG AGGGAGAAGG CCAAAGCACG AGGGGATTTC 
CAGAAGCTGC GGGAGAAGCA GCAGTTGGAG GAGGACCTAC GGGGCTACCT GGACTGGATC 
ACGCAGGCAG AGGAGCTGGA CCTCGAAGAC CCCTCAACCG ATGGCAACTT TGGTTCTATG 
GCTGAAGAGG GCCGGGCCGG CCACCGGCCA CAGCTGGCAG ATCTGACCAC CAGGAGACGT 
GGACATCTGC GCTGGTTCAG TCACTCCACC CGCTCCACAC ACTCCACCAG CAGCCATGCC 
AGCCTCCCAG CCAGTGACAC CGGTTCGATG GCAGAGATGC CAGGCGATGA GGAAGAGGAG 
GAGGGGGCTC TGGCCAGTTG TACACGCTGC CTAAACAAGA TCATGAAAAC CAGGGTCTGC 
CGCCGCCTCC GCCGAGTCAA CCGAGGACTC CGGGCACGCT GCCGGCGGGC TGTGAAGTCC 
ACCGCTTGCT ACTGGGCCGT GCTGCTGCTC GTCTTCCTCA ACACACTGAC CATAGCCTCA 
GAGCACCATG GCCAACCCAT GTGGCTTACC CAGATCCAGG AGTATGCCAA CAAGGTGTTG 
CTCTGTCTGT TCACGGTGGA GATGCTCCTC AAGTTGTACG GTCTGGGTCC TTCTGTCTAT 
GTTTCTTCCT TCTTCAACCG CTTTGACTGC TTCGTGGTCT GTGGGGGCAT CCTGGAGACC 
ACCCTGGTGG AGGTGGGCGC CATGCAGCCC CTGGGCATCT CAGTGCTCCG ATGTGTGCGC 
CTCCTCAGGA TCTTTAAGGT CACTAGGCAC TGGGCATCTC TGAGCAATTT AGTGGCATCC 
CTGCTCAATT CAATGAAATC CATCGCATCG TTGCTGCTTC TCCTCTTCCT CTTCATCATC 
ATCTTCTCCC TGCTTGGCAT GCAGCTGTTT GGGGGCAAGT TCAACTTTGA CCAGACCCAC 
ACCAAGCGGA ACACCTTTGA TACCTTCCCT CAGGCCCTCC TCACTGTCTT TCAGATCCTG 
ACATGTGAGG ATTGGAACGT GGGCATGTAT GATGGCATCA TGGCCTATGG TGGCCCCTTC 
TTCCCAGGAA TGCTGGTGTG TGTCTATTTT ATCATCCTCT TCATCTGTGG CAACTCAATC 
ACTCCCCTTT CCTCGAACAG GGAGAAGAGC ACCGAGGGGG CTCTCCCACA AGAAAATGGA 
GTGTTGGTGG CTGGTGGGGA GAATGAGGAA GAGGAGGGCA GAAAGAATGA AGGAGCAGGC 
AAGTGGCAGG GCCGGGTGGA GAAGGAAGAA GAGGAAGAGG AGGAGGGGGA GGAAGAGGAG 
GAAGGGGGTG CAGGGCATGT GGAACTCCTG CAGGAAGTTG TACCCAAGGA GAAGGTGGTA 
CCCATCCCTG AGGGCAGCGC CTTCTTCTGT CTCAGCCAAA CCAACCCGCT GAGGAAGGCC 
TGCCACACCC TCATCCACCA TCATATCTTC ACCAATCTTA TTCTGGTATT CATCATCCTC 
AGCAGTGTGT CCTTGGCCGC TGAGGACCCC ATCCGAGCCC ACTCCTTCCG CAACCATATT 
CTGGGGTATT TTGATTATGC TTTCACCTCT ATTTTCACTG TGGAGATTCT ACTAAAGATG 
ACAGTATTTG GGGCCTTCCT GCATCGAGGA TCCTTCTGCC GTAGCTGGTT CAATCTATTG 
GATCTGCTGG TGGTCAGCGT ATCCCTCATC TCCTTTGGCA TCCACTCCAG CGCCATCTCA 
GTGGTGAAAA TTCTGAGGGT ACTCCGAGTC CTGAGGCCTC TCCGAGCCAT CAACAGAGCC 
AAGGGACTCA AGCATGTTGT GCAATGTGTG TTTGTGGCTA TCCGGACCAT TGGGAATATC 
ATGATTGTGA CCACACTCCT GCAATTCATG TTTGCCTGCA TTGGCGTGCA GCTCTTCAAG 
GGGAAATTCT ACAGTTGCAC TGATGAAGCC AAACACACCC CACAAGAATG CAAGGGCTCC 
TTCCTGGTCT ACCCTGATGG AGAGGTATCA CGACCCCTGG TCCGGGAGCG GCTCTGGGTC 
AACAGCGATT TCAACTTTGA CAATGTCCTC TCAGCCATGG ATGGCCTTGT TCCTGTCTCT 
ACCTTCGAAG GTTGGCCTGC TCTACTGTAC AAGGCCATCG ATGCAAATGC AGAGGACAAG 
GGCCCTATCT ATAATTACCA TGTGGAGATC TCAGTGTTCT TCATTGTCTA CATCATCATC 
ATTGCATTCT TCATGATGAA TATCTTTGTG GGCTTTGTCA TCATCACCTT CCGTGCCCAG 
GGCGAGCAGG AATACCAAAA CTGTGAGCTG GACAAGAACC AGCGCCAGTG TGTGGAATAT 
GCCCTCAAGG CCCAGCCACT CCGCCGCTAC ATCCCCAAGA ACCCACATCA ATATCGTGTG 
TGGGCCACTG TGAACTCTGC TGCCTTCGAG TACCTCATGT TCCTGCTCAT CCTGCTCAAC 
ACCGTTGCTC TAGCAATGCA GCACTATGAA CAGACTGCTC CCTTTAATTA TGCCATGGAC 
ATCCTCAACA TGGTCTTCAC TGGCCTCTTC ACTATTGAGA TGGTGCTCAA AATCATCGCC 
TTCAAACCCA AGCATTACTT TACCGATGCC TGGAACACGT TTGATGCTCT TATTGTAGTG 
GGCAGCGTAG TGGACATTGC CGTCACTGAA GTCAACAATG GTGGCCACCT TGGCGAGAGC 
TCTGAGGACA GTTCCCGCAT TTCCATCACT TTCTTTCGCC TCTTCCGAGT CATGCGACTA 
GTCAAGCTTC TCAGTAAAGG TGAAGGAATC CGCACATTGC TCTGGACATT CATCAAGTCC 
TTCCAGGCCT TGCCCTATGT GGCTCTTCTC ATCGCAATGA TATTCTTCAT TTATGCAGTC 
ATTGGAATGC AGATGTTTGG CAAGGTGGCT CTTCAGGATG GCACACAGAT CAACCGAAAC 
AACAACTTCC AGACCTTTCC ACAGGCTGTG CTACTTTTGT TCAGGTGTGC CACCGGTGAG 
GCGTGGCAAG AGATAATGCT CGCCAGCCTT CCCGGGAGTC GGTGTGACCC TGAATCTGAC 
GTCAGCCCTG GCGAGGAGTT TACCTGTGGC AGCAATTTTG CCATCGCCTA TTTTATCAGC 
TTCTTCATGC TCTGTGCCTT CCTGATCATA AATCTCTTTG TGGCTGTCAT CATGGATAAC 
TTTGACTATC TAACCAGAGA TTGGTCCATC CTGGGCCCCC ATCACCTCGA TGAATTCAAG 
AGGATCTGGT CTGAATATGA CCCTGGAGCC AAGGGCCGCA TCAAGCACCT GGATGTGGTG 
GCCCTGCTGA GACGCATCCA GCCCCCCTTG GGATTTGGGA AGCTCTGCCC ACACCGAGTA 
GCCTGCAAGA GACTTGTGGC GATGAACATG CCCCTCAACT CAGATGGGAC AGTGACATTC 
AATGCCACAC TCTTTGCCCT GGTTCGGACA TCCTTGAAGA TCAAGACAGA AGGGAACCTG 
GAACAAGCCA ATCAGGAGCT GCGGATTGTC ATCAAAAAGA TCTGGAAGCG GATGAAGCAG 
AAGCTACTAG ATGAGGTCAT CCCCCCTGCT GACGAGGAAG AGGTCACCGT GGGCAAATTC 
TACGCCACAT TTCTGATCCA GGACTATTTC CGCAAATTCC GGCGGAGGAA AGAAAAGGGG 
CTATTAGGGA GCGAGGCCCC CTCCAGTACC TCCTCTGCCC TTCAGGCTGG TCTGAGGAGC 
CTACACGACT TGGGTCCTGA GATCAGGCAG GCCCTCACCT GTGACACAGA TGAGGAGGAG 
AAGGAGGAAG AGGGGCTGGA GGGAGAGGAG GAGGAAGATG AGAAACACCC GGAAACACAC 
AAAGCCCAGA TGGGCTCCCA GCCCCCATCT CGCCGGAGCT CCGTGATTTC TGTGTCTCTG 
CCTGTTGGGG ACAGACTTCC AGATTCACTC TCCCTTGGCC CGAGTGATGA TGATGGGGGG 
GCTTCCAATT CCAGACAGTC CAGTGTACCC CAGGCTGGAT CCCATGGCCA CAGGAGAAGC 
TCTGGGGGTT TCATTTTCAC CATCCCCGAA GAAGGAAGTT CTCAGCACAA GGGAACCAAA 
GAGCAAGAGA ATCAGGAGGA GGAAGAGGAA ATTCCCACCA AAGACTCTGG CCACAACAGG 
CTCTCCTACC TAGATGAGCA GGCAGGGACT CCCCCACGCC CCATCCTTTT GCCACCCCAC 
AGACCCCAGA GATGTGCTGA TGGGCACAAT GCACCACGCC GCCGTCTACT GCCCCCCACG 
CCTGCAGAGC GGAAGCCTTC CTTCACCATA CAGTGTCTGC GGCGCCAGGG CAGTTGTGAG 
GATTTACCCA TCCCAGGCAC CTATCATCGT GGGCGCAACT CAGGGCCCAG CAGGGCTCAG 
GGTTCTTGGG CAACCCCTCC TCAACGGGGT CGGCTCCTGT ATGCCCCACT GTTATTGGTG 
GAGGAGGGCG CAGCAGGGGA GGGATACCTC GGCAAGTCCA GCGGTCCGCT GCGTACCTTC 
ACCTGTCTGC GTGTGCCCGG AACCCACTCA GACTCCAGCC ATGGCAAGAG GGGCAGTGCT 
GACAGTCTGG TGGAGGCTGT GCTCATCTCC GAGGGCTTAG GCCTCTTTGC CCGAGACCCA 
CGCTTTGTGG CCCTAGCCAA GCAGGAGATT GCAGATGCAT GCCGCCTGAC ACTGGACGAG 
ATGGACAGTG CTGCCAGTGA CCTGTTGGCA CAGGGGACCA GCTCACTGTA TAGTGATGAG 
GAATCTATCC TCTCTCGCTT TGATGAGGAG GACCTGGGAG ATGAAATGGC CTGTGTCCAT 
GCCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1F ( XM_005642089.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005642146.1 Canis lupus familiaris calcium channel, voltage-dependent, L type, alpha 1F subunit (CACNA1F), mRNA.

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CloneID	OCe33235
Clone ID Related Accession (Same CDS sequence)	XM_005642089.2
Accession Version	XM_005642089.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5916bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	LOW QUALITY PROTEIN: voltage-dependent L-type calcium channel subunit alpha-1F
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005642089.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGTCGGCAT CTGATGGCGG GAAAGACACC ACCCCAGAGC CCAGCCCAGT CAATGGGACA 
GGCCCTGGGC CTGAATGGGG GCTGTGTCCT GGGCCTCCAG CATCAGGGGG TGAAGAAATC 
AGCGGGGCCG TGGGCCCAGA GACCCCTAAG CGAAGAACTC AGCACAATAA GCACAAGACG 
GTGGCAGTGG CCAGTGCCCA ACGGTCACCC CGGGCGCTCT TCTGCCTCAC TCTGGCCAAC 
CCGCTGCGGC GGTCCTGCAT CAGCATTGTG GAGTGGAAGC CCTTTGACAT CCTCATCCTG 
CTGACCATCT TTGCCAACTG TGTAGCCCTG GGGGTCTACA TCCCCTTCCC AGAGGATGAC 
TCCAACACTG CTAACCACAA CCTGGAGCAA GTGGAGTATG TATTCCTGGT GATTTTCACT 
GTGGAAACCG TGCTCAAGAT CGTGGCCTAT GGGCTGGTGC TCCATCCCAG CGCCTACATC 
CGCAACGGCT GGAACCTACT TGACTTCATC ATCGTTGTGG TCGGGCTGTT CAGCGTGCTA 
CTTGAGCAGG GCCCTGGTCG GCCGGGGGAC ACCCCGCATA CCGGGGGGAA GCCGGGGGGC 
TTCGATGTGA AGGCGTTGAG GGCGTTTCGG GTGCTGCGGC CACTGAGGCT GGTATCTGGG 
GTGCCAAGCC TGCACATAGT GCTCAATTCC ATCATGAAGG CGCTGGTGCC GCTACTGCAC 
ATTGCACTGC TCGTGCTCTT CGTCATCATC ATCTATGCCA TCATCGGACT CGAGCTGTTC 
CTTGGACGCA TGCACAAGAC ATGTTACTTC CTGGGATCTG ACCTGGAAGC GGAGGAGGAC 
CCGTCGCCCT GTGCGTCTTC GGGCNTCGGG CGAGCGTGCA CGCTGAACCA GACCGAGTGC 
CGGGGGCGCT GGGCAGGGCC CAACGGAGGC ATCACCAACT TCGACAACTT CTTCTTCGCC 
ATGCTCACGG TCTTCCAGTG CGTCACCATG GAAGGCTGGA CCGACGTGCT CTACTGGATG 
CAGGACGCCA TGGGGTATGA GCTGCCCTGG GTATATTTCG TGAGTCTCGT CATCTTCGGG 
TCCTTCTTCG TCCTCAACCT TGTGCTTGGT GTCCTGAGTG GGGAGTTCTC CAAGGAGAGG 
GAGAAGGCCA AAGCACGAGG GGATTTCCAG AAGCTGCGGG AGAAGCAGCA GTTGGAGGAG 
GACCTACGGG GCTACCTGGA CTGGATCACG CAGGCAGAGG AGCTGGACCT CGAAGACCCC 
TCAACCGATG GCAACTTTGG TTCTATGGCT GAAGAGGGCC GGGCCGGCCA CCGGCCACAG 
CTGGCAGATC TGACCACCAG GAGACGTGGA CATCTGCGCT GGTTCAGTCA CTCCACCCGC 
TCCACACACT CCACCAGCAG CCATGCCAGC CTCCCAGCCA GTGACACCGG TTCGATGGCA 
GAGATGCCAG GCGATGAGGA AGAGGAGGAG GGGGCTCTGG CCAGTTGTAC ACGCTGCCTA 
AACAAGATCA TGAAAACCAG GGTCTGCCGC CGCCTCCGCC GAGTCAACCG AGGACTCCGG 
GCACGCTGCC GGCGGGCTGT GAAGTCCACC GCTTGCTACT GGGCCGTGCT GCTGCTCGTC 
TTCCTCAACA CACTGACCAT AGCCTCAGAG CACCATGGCC AACCCATGTG GCTTACCCAG 
ATCCAGGAGT ATGCCAACAA GGTGTTGCTC TGTCTGTTCA CGGTGGAGAT GCTCCTCAAG 
TTGTACGGTC TGGGTCCTTC TGTCTATGTT TCTTCCTTCT TCAACCGCTT TGACTGCTTC 
GTGGTCTGTG GGGGCATCCT GGAGACCACC CTGGTGGAGG TGGGCGCCAT GCAGCCCCTG 
GGCATCTCAG TGCTCCGATG TGTGCGCCTC CTCAGGATCT TTAAGGTCAC TAGGCACTGG 
GCATCTCTGA GCAATTTAGT GGCATCCCTG CTCAATTCAA TGAAATCCAT CGCATCGTTG 
CTGCTTCTCC TCTTCCTCTT CATCATCATC TTCTCCCTGC TTGGCATGCA GCTGTTTGGG 
GGCAAGTTCA ACTTTGACCA GACCCACACC AAGCGGAACA CCTTTGATAC CTTCCCTCAG 
GCCCTCCTCA CTGTCTTTCA GATCCTGACA TGTGAGGATT GGAACGTGGG CATGTATGAT 
GGCATCATGG CCTATGGTGG CCCCTTCTTC CCAGGAATGC TGGTGTGTGT CTATTTTATC 
ATCCTCTTCA TCTGTGGCAA CTCAATCACT CCCCTTTCCT CGAACAGGGA GAAGAGCACC 
GAGGGGGCTC TCCCACAAGA AAATGGAGTG TTGGTGGCTG GTGGGGAGAA TGAGGAAGAG 
GAGGGCAGAA AGAATGAAGG AGCAGGCAAG TGGCAGGGCC GGGTGGAGAA GGAAGAAGAG 
GAAGAGGAGG AGGGGGAGGA AGAGGAGGAA GGGGGTGCAG GGCATGTGGA ACTCCTGCAG 
GAAGTTGTAC CCAAGGAGAA GGTGGTACCC ATCCCTGAGG GCAGCGCCTT CTTCTGTCTC 
AGCCAAACCA ACCCGCTGAG GAAGGCCTGC CACACCCTCA TCCACCATCA TATCTTCACC 
AATCTTATTC TGGTATTCAT CATCCTCAGC AGTGTGTCCT TGGCCGCTGA GGACCCCATC 
CGAGCCCACT CCTTCCGCAA CCATATTCTG GGGTATTTTG ATTATGCTTT CACCTCTATT 
TTCACTGTGG AGATTCTACT AAAGATGACA GTATTTGGGG CCTTCCTGCA TCGAGGATCC 
TTCTGCCGTA GCTGGTTCAA TCTATTGGAT CTGCTGGTGG TCAGCGTATC CCTCATCTCC 
TTTGGCATCC ACTCCAGCGC CATCTCAGTG GTGAAAATTC TGAGGGTACT CCGAGTCCTG 
AGGCCTCTCC GAGCCATCAA CAGAGCCAAG GGACTCAAGC ATGTTGTGCA ATGTGTGTTT 
GTGGCTATCC GGACCATTGG GAATATCATG ATTGTGACCA CACTCCTGCA ATTCATGTTT 
GCCTGCATTG GCGTGCAGCT CTTCAAGGGG AAATTCTACA GTTGCACTGA TGAAGCCAAA 
CACACCCCAC AAGAATGCAA GGGCTCCTTC CTGGTCTACC CTGATGGAGA GGTATCACGA 
CCCCTGGTCC GGGAGCGGCT CTGGGTCAAC AGCGATTTCA ACTTTGACAA TGTCCTCTCA 
GCCATGGATG GCCTTGTTCC TGTCTCTACC TTCGAAGGTT GGCCTGCTCT ACTGTACAAG 
GCCATCGATG CAAATGCAGA GGACAAGGGC CCTATCTATA ATTACCATGT GGAGATCTCA 
GTGTTCTTCA TTGTCTACAT CATCATCATT GCATTCTTCA TGATGAATAT CTTTGTGGGC 
TTTGTCATCA TCACCTTCCG TGCCCAGGGC GAGCAGGAAT ACCAAAACTG TGAGCTGGAC 
AAGAACCAGC GCCAGTGTGT GGAATATGCC CTCAAGGCCC AGCCACTCCG CCGCTACATC 
CCCAAGAACC CACATCAATA TCGTGTGTGG GCCACTGTGA ACTCTGCTGC CTTCGAGTAC 
CTCATGTTCC TGCTCATCCT GCTCAACACC GTTGCTCTAG CAATGCAGCA CTATGAACAG 
ACTGCTCCCT TTAATTATGC CATGGACATC CTCAACATGG TCTTCACTGG CCTCTTCACT 
ATTGAGATGG TGCTCAAAAT CATCGCCTTC AAACCCAAGC ATTACTTTAC CGATGCCTGG 
AACACGTTTG ATGCTCTTAT TGTAGTGGGC AGCGTAGTGG ACATTGCCGT CACTGAAGTC 
AACAATGGTG GCCACCTTGG CGAGAGCTCT GAGGACAGTT CCCGCATTTC CATCACTTTC 
TTTCGCCTCT TCCGAGTCAT GCGACTAGTC AAGCTTCTCA GTAAAGGTGA AGGAATCCGC 
ACATTGCTCT GGACATTCAT CAAGTCCTTC CAGGCCTTGC CCTATGTGGC TCTTCTCATC 
GCAATGATAT TCTTCATTTA TGCAGTCATT GGAATGCAGA TGTTTGGCAA GGTGGCTCTT 
CAGGATGGCA CACAGATCAA CCGAAACAAC AACTTCCAGA CCTTTCCACA GGCTGTGCTA 
CTTTTGTTCA GGTGTGCCAC CGGTGAGGCG TGGCAAGAGA TAATGCTCGC CAGCCTTCCC 
GGGAGTCGGT GTGACCCTGA ATCTGACGTC AGCCCTGGCG AGGAGTTTAC CTGTGGCAGC 
AATTTTGCCA TCGCCTATTT TATCAGCTTC TTCATGCTCT GTGCCTTCCT GATCATAAAT 
CTCTTTGTGG CTGTCATCAT GGATAACTTT GACTATCTAA CCAGAGATTG GTCCATCCTG 
GGCCCCCATC ACCTCGATGA ATTCAAGAGG ATCTGGTCTG AATATGACCC TGGAGCCAAG 
GGCCGCATCA AGCACCTGGA TGTGGTGGCC CTGCTGAGAC GCATCCAGCC CCCCTTGGGA 
TTTGGGAAGC TCTGCCCACA CCGAGTAGCC TGCAAGAGAC TTGTGGCGAT GAACATGCCC 
CTCAACTCAG ATGGGACAGT GACATTCAAT GCCACACTCT TTGCCCTGGT TCGGACATCC 
TTGAAGATCA AGACAGAAGG GAACCTGGAA CAAGCCAATC AGGAGCTGCG GATTGTCATC 
AAAAAGATCT GGAAGCGGAT GAAGCAGAAG CTACTAGATG AGGTCATCCC CCCTGCTGAC 
GAGGAAGAGG TCACCGTGGG CAAATTCTAC GCCACATTTC TGATCCAGGA CTATTTCCGC 
AAATTCCGGC GGAGGAAAGA AAAGGGGCTA TTAGGGAGCG AGGCCCCCTC CAGTACCTCC 
TCTGCCCTTC AGGCTGGTCT GAGGAGCCTA CACGACTTGG GTCCTGAGAT CAGGCAGGCC 
CTCACCTGTG ACACAGATGA GGAGGAGAAG GAGGAAGAGG GGCTGGAGGG AGAGGAGGAG 
GAAGATGAGA AACACCCGGA AACACACAAA GCCCAGATGG GCTCCCAGCC CCCATCTCGC 
CGGAGCTCCG TGATTTCTGT GTCTCTGCCT GTTGGGGACA GACTTCCAGA TTCACTCTCC 
CTTGGCCCGA GTGATGATGA TGGGGGGGCT TCCAATTCCA GACAGTCCAG TGTACCCCAG 
GCTGGATCCC ATGGCCACAG GAGAAGCTCT GGGGGTTTCA TTTTCACCAT CCCCGAAGAA 
GGAAGTTCTC AGCACAAGGG AACCAAAGAG CAAGAGAATC AGGAGGAGGA AGAGGAAATT 
CCCACCAAAG ACTCTGGCCA CAACAGGCTC TCCTACCTAG ATGAGCAGGC AGGGACTCCC 
CCACGCCCCA TCCTTTTGCC ACCCCACAGA CCCCAGAGAT GTGCTGATGG GCACAATGCA 
CCACGCCGCC GTCTACTGCC CCCCACGCCT GCAGAGCGGA AGCCTTCCTT CACCATACAG 
TGTCTGCGGC GCCAGGGCAG TTGTGAGGAT TTACCCATCC CAGGCACCTA TCATCGTGGG 
CGCAACTCAG GGCCCAGCAG GGCTCAGGGT TCTTGGGCAA CCCCTCCTCA ACGGGGTCGG 
CTCCTGTATG CCCCACTGTT ATTGGTGGAG GAGGGCGCAG CAGGGGAGGG ATACCTCGGC 
AAGTCCAGCG GTCCGCTGCG TACCTTCACC TGTCTGCGTG TGCCCGGAAC CCACTCAGAC 
TCCAGCCATG GCAAGAGGGG CAGTGCTGAC AGTCTGGTGG AGGCTGTGCT CATCTCCGAG 
GGCTTAGGCC TCTTTGCCCG AGACCCACGC TTTGTGGCCC TAGCCAAGCA GGAGATTGCA 
GATGCATGCC GCCTGACACT GGACGAGATG GACAGTGCTG CCAGTGACCT GTTGGCACAG 
GGGACCAGCT CACTGTATAG TGATGAGGAA TCTATCCTCT CTCGCTTTGA TGAGGAGGAC 
CTGGGAGATG AAATGGCCTG TGTCCATGCC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005642146.1
CDS	1..5916
Translation	MSASDGGKDTTPEPSPVNGTGPGPEWGLCPGPPASGGEEISGAVGPETPKRRTQHNKHKTVAVASAQRSPRALFCLTLANPLRRSCISIVEWKPFDILILLTIFANCVALGVYIPFPEDDSNTANHNLEQVEYVFLVIFTVETVLKIVAYGLVLHPSAYIRNGWNLLDFIIVVVGLFSVLLEQGPGRPGDTPHTGGKPGGFDVKALRAFRVLRPLRLVSGVPSLHIVLNSIMKALVPLLHIALLVLFVIIIYAIIGLELFLGRMHKTCYFLGSDLEAEEDPSPCASSGnullGRACTLNQTECRGRWAGPNGGITNFDNFFFAMLTVFQCVTMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLRGYLDWITQAEELDLEDPSTDGNFGSMAEEGRAGHRPQLADLTTRRRGHLRWFSHSTRSTHSTSSHASLPASDTGSMAEMPGDEEEEEGALASCTRCLNKIMKTRVCRRLRRVNRGLRARCRRAVKSTACYWAVLLLVFLNTLTIASEHHGQPMWLTQIQEYANKVLLCLFTVEMLLKLYGLGPSVYVSSFFNRFDCFVVCGGILETTLVEVGAMQPLGISVLRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDQTHTKRNTFDTFPQALLTVFQILTCEDWNVGMYDGIMAYGGPFFPGMLVCVYFIILFICGNSITPLSSNREKSTEGALPQENGVLVAGGENEEEEGRKNEGAGKWQGRVEKEEEEEEEGEEEEEGGAGHVELLQEVVPKEKVVPIPEGSAFFCLSQTNPLRKACHTLIHHHIFTNLILVFIILSSVSLAAEDPIRAHSFRNHILGYFDYAFTSIFTVEILLKMTVFGAFLHRGSFCRSWFNLLDLLVVSVSLISFGIHSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYSCTDEAKHTPQECKGSFLVYPDGEVSRPLVRERLWVNSDFNFDNVLSAMDGLVPVSTFEGWPALLYKAIDANAEDKGPIYNYHVEISVFFIVYIIIIAFFMMNIFVGFVIITFRAQGEQEYQNCELDKNQRQCVEYALKAQPLRRYIPKNPHQYRVWATVNSAAFEYLMFLLILLNTVALAMQHYEQTAPFNYAMDILNMVFTGLFTIEMVLKIIAFKPKHYFTDAWNTFDALIVVGSVVDIAVTEVNNGGHLGESSEDSSRISITFFRLFRVMRLVKLLSKGEGIRTLLWTFIKSFQALPYVALLIAMIFFIYAVIGMQMFGKVALQDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLASLPGSRCDPESDVSPGEEFTCGSNFAIAYFISFFMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPGAKGRIKHLDVVALLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVTFNATLFALVRTSLKIKTEGNLEQANQELRIVIKKIWKRMKQKLLDEVIPPADEEEVTVGKFYATFLIQDYFRKFRRRKEKGLLGSEAPSSTSSALQAGLRSLHDLGPEIRQALTCDTDEEEKEEEGLEGEEEEDEKHPETHKAQMGSQPPSRRSSVISVSLPVGDRLPDSLSLGPSDDDGGASNSRQSSVPQAGSHGHRRSSGGFIFTIPEEGSSQHKGTKEQENQEEEEEIPTKDSGHNRLSYLDEQAGTPPRPILLPPHRPQRCADGHNAPRRRLLPPTPAERKPSFTIQCLRRQGSCEDLPIPGTYHRGRNSGPSRAQGSWATPPQRGRLLYAPLLLVEEGAAGEGYLGKSSGPLRTFTCLRVPGTHSDSSHGKRGSADSLVEAVLISEGLGLFARDPRFVALAKQEIADACRLTLDEMDSAASDLLAQGTSSLYSDEESILSRFDEEDLGDEMACVHAL

Target ORF information:

RefSeq Version	XM_005642089.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris calcium channel, voltage-dependent, L type, alpha 1F subunit (CACNA1F), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005642089.2

ORF Insert Sequence:

ATGTCGGCAT CTGATGGCGG GAAAGACACC ACCCCAGAGC CCAGCCCAGT CAATGGGACA 
GGCCCTGGGC CTGAATGGGG GCTGTGTCCT GGGCCTCCAG CATCAGGGGG TGAAGAAATC 
AGCGGGGCCG TGGGCCCAGA GACCCCTAAG CGAAGAACTC AGCACAATAA GCACAAGACG 
GTGGCAGTGG CCAGTGCCCA ACGGTCACCC CGGGCGCTCT TCTGCCTCAC TCTGGCCAAC 
CCGCTGCGGC GGTCCTGCAT CAGCATTGTG GAGTGGAAGC CCTTTGACAT CCTCATCCTG 
CTGACCATCT TTGCCAACTG TGTAGCCCTG GGGGTCTACA TCCCCTTCCC AGAGGATGAC 
TCCAACACTG CTAACCACAA CCTGGAGCAA GTGGAGTATG TATTCCTGGT GATTTTCACT 
GTGGAAACCG TGCTCAAGAT CGTGGCCTAT GGGCTGGTGC TCCATCCCAG CGCCTACATC 
CGCAACGGCT GGAACCTACT TGACTTCATC ATCGTTGTGG TCGGGCTGTT CAGCGTGCTA 
CTTGAGCAGG GCCCTGGTCG GCCGGGGGAC ACCCCGCATA CCGGGGGGAA GCCGGGGGGC 
TTCGATGTGA AGGCGTTGAG GGCGTTTCGG GTGCTGCGGC CACTGAGGCT GGTATCTGGG 
GTGCCAAGCC TGCACATAGT GCTCAATTCC ATCATGAAGG CGCTGGTGCC GCTACTGCAC 
ATTGCACTGC TCGTGCTCTT CGTCATCATC ATCTATGCCA TCATCGGACT CGAGCTGTTC 
CTTGGACGCA TGCACAAGAC ATGTTACTTC CTGGGATCTG ACCTGGAAGC GGAGGAGGAC 
CCGTCGCCCT GTGCGTCTTC GGGCNTCGGG CGAGCGTGCA CGCTGAACCA GACCGAGTGC 
CGGGGGCGCT GGGCAGGGCC CAACGGAGGC ATCACCAACT TCGACAACTT CTTCTTCGCC 
ATGCTCACGG TCTTCCAGTG CGTCACCATG GAAGGCTGGA CCGACGTGCT CTACTGGATG 
CAGGACGCCA TGGGGTATGA GCTGCCCTGG GTATATTTCG TGAGTCTCGT CATCTTCGGG 
TCCTTCTTCG TCCTCAACCT TGTGCTTGGT GTCCTGAGTG GGGAGTTCTC CAAGGAGAGG 
GAGAAGGCCA AAGCACGAGG GGATTTCCAG AAGCTGCGGG AGAAGCAGCA GTTGGAGGAG 
GACCTACGGG GCTACCTGGA CTGGATCACG CAGGCAGAGG AGCTGGACCT CGAAGACCCC 
TCAACCGATG GCAACTTTGG TTCTATGGCT GAAGAGGGCC GGGCCGGCCA CCGGCCACAG 
CTGGCAGATC TGACCACCAG GAGACGTGGA CATCTGCGCT GGTTCAGTCA CTCCACCCGC 
TCCACACACT CCACCAGCAG CCATGCCAGC CTCCCAGCCA GTGACACCGG TTCGATGGCA 
GAGATGCCAG GCGATGAGGA AGAGGAGGAG GGGGCTCTGG CCAGTTGTAC ACGCTGCCTA 
AACAAGATCA TGAAAACCAG GGTCTGCCGC CGCCTCCGCC GAGTCAACCG AGGACTCCGG 
GCACGCTGCC GGCGGGCTGT GAAGTCCACC GCTTGCTACT GGGCCGTGCT GCTGCTCGTC 
TTCCTCAACA CACTGACCAT AGCCTCAGAG CACCATGGCC AACCCATGTG GCTTACCCAG 
ATCCAGGAGT ATGCCAACAA GGTGTTGCTC TGTCTGTTCA CGGTGGAGAT GCTCCTCAAG 
TTGTACGGTC TGGGTCCTTC TGTCTATGTT TCTTCCTTCT TCAACCGCTT TGACTGCTTC 
GTGGTCTGTG GGGGCATCCT GGAGACCACC CTGGTGGAGG TGGGCGCCAT GCAGCCCCTG 
GGCATCTCAG TGCTCCGATG TGTGCGCCTC CTCAGGATCT TTAAGGTCAC TAGGCACTGG 
GCATCTCTGA GCAATTTAGT GGCATCCCTG CTCAATTCAA TGAAATCCAT CGCATCGTTG 
CTGCTTCTCC TCTTCCTCTT CATCATCATC TTCTCCCTGC TTGGCATGCA GCTGTTTGGG 
GGCAAGTTCA ACTTTGACCA GACCCACACC AAGCGGAACA CCTTTGATAC CTTCCCTCAG 
GCCCTCCTCA CTGTCTTTCA GATCCTGACA TGTGAGGATT GGAACGTGGG CATGTATGAT 
GGCATCATGG CCTATGGTGG CCCCTTCTTC CCAGGAATGC TGGTGTGTGT CTATTTTATC 
ATCCTCTTCA TCTGTGGCAA CTCAATCACT CCCCTTTCCT CGAACAGGGA GAAGAGCACC 
GAGGGGGCTC TCCCACAAGA AAATGGAGTG TTGGTGGCTG GTGGGGAGAA TGAGGAAGAG 
GAGGGCAGAA AGAATGAAGG AGCAGGCAAG TGGCAGGGCC GGGTGGAGAA GGAAGAAGAG 
GAAGAGGAGG AGGGGGAGGA AGAGGAGGAA GGGGGTGCAG GGCATGTGGA ACTCCTGCAG 
GAAGTTGTAC CCAAGGAGAA GGTGGTACCC ATCCCTGAGG GCAGCGCCTT CTTCTGTCTC 
AGCCAAACCA ACCCGCTGAG GAAGGCCTGC CACACCCTCA TCCACCATCA TATCTTCACC 
AATCTTATTC TGGTATTCAT CATCCTCAGC AGTGTGTCCT TGGCCGCTGA GGACCCCATC 
CGAGCCCACT CCTTCCGCAA CCATATTCTG GGGTATTTTG ATTATGCTTT CACCTCTATT 
TTCACTGTGG AGATTCTACT AAAGATGACA GTATTTGGGG CCTTCCTGCA TCGAGGATCC 
TTCTGCCGTA GCTGGTTCAA TCTATTGGAT CTGCTGGTGG TCAGCGTATC CCTCATCTCC 
TTTGGCATCC ACTCCAGCGC CATCTCAGTG GTGAAAATTC TGAGGGTACT CCGAGTCCTG 
AGGCCTCTCC GAGCCATCAA CAGAGCCAAG GGACTCAAGC ATGTTGTGCA ATGTGTGTTT 
GTGGCTATCC GGACCATTGG GAATATCATG ATTGTGACCA CACTCCTGCA ATTCATGTTT 
GCCTGCATTG GCGTGCAGCT CTTCAAGGGG AAATTCTACA GTTGCACTGA TGAAGCCAAA 
CACACCCCAC AAGAATGCAA GGGCTCCTTC CTGGTCTACC CTGATGGAGA GGTATCACGA 
CCCCTGGTCC GGGAGCGGCT CTGGGTCAAC AGCGATTTCA ACTTTGACAA TGTCCTCTCA 
GCCATGGATG GCCTTGTTCC TGTCTCTACC TTCGAAGGTT GGCCTGCTCT ACTGTACAAG 
GCCATCGATG CAAATGCAGA GGACAAGGGC CCTATCTATA ATTACCATGT GGAGATCTCA 
GTGTTCTTCA TTGTCTACAT CATCATCATT GCATTCTTCA TGATGAATAT CTTTGTGGGC 
TTTGTCATCA TCACCTTCCG TGCCCAGGGC GAGCAGGAAT ACCAAAACTG TGAGCTGGAC 
AAGAACCAGC GCCAGTGTGT GGAATATGCC CTCAAGGCCC AGCCACTCCG CCGCTACATC 
CCCAAGAACC CACATCAATA TCGTGTGTGG GCCACTGTGA ACTCTGCTGC CTTCGAGTAC 
CTCATGTTCC TGCTCATCCT GCTCAACACC GTTGCTCTAG CAATGCAGCA CTATGAACAG 
ACTGCTCCCT TTAATTATGC CATGGACATC CTCAACATGG TCTTCACTGG CCTCTTCACT 
ATTGAGATGG TGCTCAAAAT CATCGCCTTC AAACCCAAGC ATTACTTTAC CGATGCCTGG 
AACACGTTTG ATGCTCTTAT TGTAGTGGGC AGCGTAGTGG ACATTGCCGT CACTGAAGTC 
AACAATGGTG GCCACCTTGG CGAGAGCTCT GAGGACAGTT CCCGCATTTC CATCACTTTC 
TTTCGCCTCT TCCGAGTCAT GCGACTAGTC AAGCTTCTCA GTAAAGGTGA AGGAATCCGC 
ACATTGCTCT GGACATTCAT CAAGTCCTTC CAGGCCTTGC CCTATGTGGC TCTTCTCATC 
GCAATGATAT TCTTCATTTA TGCAGTCATT GGAATGCAGA TGTTTGGCAA GGTGGCTCTT 
CAGGATGGCA CACAGATCAA CCGAAACAAC AACTTCCAGA CCTTTCCACA GGCTGTGCTA 
CTTTTGTTCA GGTGTGCCAC CGGTGAGGCG TGGCAAGAGA TAATGCTCGC CAGCCTTCCC 
GGGAGTCGGT GTGACCCTGA ATCTGACGTC AGCCCTGGCG AGGAGTTTAC CTGTGGCAGC 
AATTTTGCCA TCGCCTATTT TATCAGCTTC TTCATGCTCT GTGCCTTCCT GATCATAAAT 
CTCTTTGTGG CTGTCATCAT GGATAACTTT GACTATCTAA CCAGAGATTG GTCCATCCTG 
GGCCCCCATC ACCTCGATGA ATTCAAGAGG ATCTGGTCTG AATATGACCC TGGAGCCAAG 
GGCCGCATCA AGCACCTGGA TGTGGTGGCC CTGCTGAGAC GCATCCAGCC CCCCTTGGGA 
TTTGGGAAGC TCTGCCCACA CCGAGTAGCC TGCAAGAGAC TTGTGGCGAT GAACATGCCC 
CTCAACTCAG ATGGGACAGT GACATTCAAT GCCACACTCT TTGCCCTGGT TCGGACATCC 
TTGAAGATCA AGACAGAAGG GAACCTGGAA CAAGCCAATC AGGAGCTGCG GATTGTCATC 
AAAAAGATCT GGAAGCGGAT GAAGCAGAAG CTACTAGATG AGGTCATCCC CCCTGCTGAC 
GAGGAAGAGG TCACCGTGGG CAAATTCTAC GCCACATTTC TGATCCAGGA CTATTTCCGC 
AAATTCCGGC GGAGGAAAGA AAAGGGGCTA TTAGGGAGCG AGGCCCCCTC CAGTACCTCC 
TCTGCCCTTC AGGCTGGTCT GAGGAGCCTA CACGACTTGG GTCCTGAGAT CAGGCAGGCC 
CTCACCTGTG ACACAGATGA GGAGGAGAAG GAGGAAGAGG GGCTGGAGGG AGAGGAGGAG 
GAAGATGAGA AACACCCGGA AACACACAAA GCCCAGATGG GCTCCCAGCC CCCATCTCGC 
CGGAGCTCCG TGATTTCTGT GTCTCTGCCT GTTGGGGACA GACTTCCAGA TTCACTCTCC 
CTTGGCCCGA GTGATGATGA TGGGGGGGCT TCCAATTCCA GACAGTCCAG TGTACCCCAG 
GCTGGATCCC ATGGCCACAG GAGAAGCTCT GGGGGTTTCA TTTTCACCAT CCCCGAAGAA 
GGAAGTTCTC AGCACAAGGG AACCAAAGAG CAAGAGAATC AGGAGGAGGA AGAGGAAATT 
CCCACCAAAG ACTCTGGCCA CAACAGGCTC TCCTACCTAG ATGAGCAGGC AGGGACTCCC 
CCACGCCCCA TCCTTTTGCC ACCCCACAGA CCCCAGAGAT GTGCTGATGG GCACAATGCA 
CCACGCCGCC GTCTACTGCC CCCCACGCCT GCAGAGCGGA AGCCTTCCTT CACCATACAG 
TGTCTGCGGC GCCAGGGCAG TTGTGAGGAT TTACCCATCC CAGGCACCTA TCATCGTGGG 
CGCAACTCAG GGCCCAGCAG GGCTCAGGGT TCTTGGGCAA CCCCTCCTCA ACGGGGTCGG 
CTCCTGTATG CCCCACTGTT ATTGGTGGAG GAGGGCGCAG CAGGGGAGGG ATACCTCGGC 
AAGTCCAGCG GTCCGCTGCG TACCTTCACC TGTCTGCGTG TGCCCGGAAC CCACTCAGAC 
TCCAGCCATG GCAAGAGGGG CAGTGCTGAC AGTCTGGTGG AGGCTGTGCT CATCTCCGAG 
GGCTTAGGCC TCTTTGCCCG AGACCCACGC TTTGTGGCCC TAGCCAAGCA GGAGATTGCA 
GATGCATGCC GCCTGACACT GGACGAGATG GACAGTGCTG CCAGTGACCT GTTGGCACAG 
GGGACCAGCT CACTGTATAG TGATGAGGAA TCTATCCTCT CTCGCTTTGA TGAGGAGGAC 
CTGGGAGATG AAATGGCCTG TGTCCATGCC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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CACNA1F cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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