CFTR cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	CFTR
Entrez Gene ID	281067
Full Name	cystic fibrosis transmembrane conductance regulator
General protein information	Preferred Names cystic fibrosis transmembrane conductance regulator Names cystic fibrosis transmembrane conductance regulator ATP-binding cassette sub-family C member 7 ATP-binding cassette transporter sub-family C member 7 cAMP-dependent chloride channel channel conductance-controlling ATPase cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7)
Gene Type	protein-coding
Organism	Bos taurus(cattle)
Genome	Chromosome: 4 Map Location: 4q23-q25

mRNA and Protein(s)

mRNA	Protein	Name
NM_174018.2	NP_776443.1	cystic fibrosis transmembrane conductance regulator

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	CFTR	NP_001028110.1
Pan troglodytes (chimpanzee)	CFTR	NP_001073386.1
Rattus norvegicus (Norway rat)	Cftr	NP_113694.1
Canis lupus familiaris (dog)	CFTR	NP_001007144.1
Mus musculus (house mouse)	Cftr	NP_066388.1
Xenopus tropicalis (tropical clawed frog)	cftr	XP_002935002.2
Homo sapiens (human)	CFTR	NP_000483.3
Bos taurus (cattle)	CFTR	NP_776443.1
Danio rerio (zebrafish)	cftr	NP_001038348.1
Gallus gallus (chicken)	CFTR	NP_001099136.1

Gene Ontology
Bibliography

Related articles in PubMed

Missense mutation within cystic fibrosis transmembrane conductance regulator (CFTR) gene is associated with selected parameters of the frozen-thawed sperm in Holstein-Friesian bulls.
Kaminski S, Hering DM, Kordan W, Lecewicz M
Polish journal of veterinary sciences22(2)221-225(2019 Jun)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Marçais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

A comparative genomic analysis of the cow, pig, and human CFTR genes identifies potential intronic regulatory elements.
Williams SH, Mouchel N, Harris A
Genomics81(6)628-39(2003 Jun)

Phosphorylation of protein kinase C sites in NBD1 and the R domain control CFTR channel activation by PKA.
Chappe V, Hinkson DA, Zhu T, Chang XB, Riordan JR, Hanrahan JW
The Journal of physiology548(Pt 1)39-52(2003 Apr)

Estimate of nucleotide diversity in dogs with a pool-and-sequence method.
Brouillette JA, Andrew JR, Venta PJ
Mammalian genome : official journal of the International Mammalian Genome Society11(12)1079-86(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CFTR gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CFTR cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa41444	NM_174018.2 Latest version!	Bos taurus CF transmembrane conductance regulator (CFTR), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CFTR ( NM_174018.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776443.1 Bos taurus CF transmembrane conductance regulator (CFTR), mRNA.

Price & Availability↑

CloneID	OBa41444
Clone ID Related Accession (Same CDS sequence)	NM_174018.2
Accession Version	NM_174018.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4446bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577289600000
Organism	Bos taurus(cattle)
Product	cystic fibrosis transmembrane conductance regulator
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M76128.1. On Jun 3, 2003 this sequence version replaced NM_174018.1. ##Evidence-Data-START## Transcript exon combination :: M76128.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145482 [ECO:0000348] ##Evidence-Data-END##

ATGCAGAGGT CGCCTCTGGA AAAGGCCAGC GTCGTCTCCA AAGTTTTTTT CAGCTGGACC 
AGACCAATTT TGAAGAAAGG ATACAGACAG CGCCTGGAAT TGTCAGACAT ATACCATATC 
TCTTCTTCTG ATTCTGCTGA CAATCTATCT GAAAAATTGG AAAGAGAATG GGACAGAGAA 
CTGGCTTCAA AGAAAAATCC CAAACTCATA AATGCCCTTC GACGATGCTT TTTCTGGAGA 
TTTATGTTCT ATGGAATCAT ATTATATTTA GGGGAAGTCA CCAAAGCAGT CCAGCCTCTC 
TTACTGGGAA GAATCATAGC TTCCTATGAC CCAGATAACA AGGTGGAACG CTCCATTGCC 
ATTTACTTAG GCATAGGCCT ATGCCTTCTC TTTATCGTGA GGACACTGCT CCTGCACCCA 
GCCATTTTTG GTCTCCATCA CATTGGAATG CAGATGAGAA TAGCTATGTT TAGTTTGATT 
TATAAGAAGA CTTTGAAGCT ATCAAGCCGT GTTCTGGATA AAATAAGTAT TGGACGACTT 
GTTAGTCTCC TTTCCAACAA CCTGAACAAA TTTGATGAAG GACTCGCACT GGCACATTTT 
GTGTGGATCG CTCCTCTGCA AGTGACGCTG CTCATGGGGC TGCTCTGGGA GTTGTTGCAG 
GCCTTCACCT TCTGTGGGCT TGCTTTCCTC ATAGTCCTCG CCCTTCTTCA AGCCGGATTA 
GGGAAAATGA TGATGAAGTA CAGAGATCAG AGAGCTGGAA AGATCAATGA AAGAGTGGTG 
ATAACGTCAG AAATGATTGA AAATATCCAA TCAGTTAAGG CATACTGCTG GGAAGAAGCA 
ATGGAAAAAA TCATTGAAAA CCTAAGACAA ACAGAACTGA AACTCACCCG GAAGGCAGCC 
TATGTGAGAT ACCTCAATAG CTCAGCCTTC TTCTTCTCAG GGTTCTTTGT GGTATTTTTA 
TCTGTGCTTC CTTATGCACT GCTCAAAGGA ATCATCCTTC GAAAAATATT CACAACCATC 
TCATTCTGCA TCGTTCTGCG CATGGCAGTT ACTCGGCAGT TCCCCTGGGC TGTACAAACT 
TGGTATGATT CTCTGGGAGC AATAAACAAA ATACAGGATT TCTTACAAAA GCAAGAATAT 
AAGACATTGG AATATAACTT AACAACTACA GATGTAGTGA TGGATAATGT AACAGCCTTC 
TGGGAGGAGG GATTTAGTAA ATTATTTGAG AAAGCAAAAG AAAATAATAA CAATAGAAAA 
ATTTCCAATG GTGATAACAG CCTCTTCTTC AGTAACTTAC TTCTTGGTAC TCCTGTCCTG 
AAAGATATCA GTTTCAAGAT AGAAAGAGGA CAGTTGTTGG CAGTTGCTGG ATCTACGGGA 
GCAGGCAAGA CTTCACTTCT CTTGATGATT ATGGGAGAAT TGGAAGCTTC AGAGGGTAAA 
ATTAAGCACA GTGGAAGAAT TTCATTCTGC TCTCAGTATT CCTGGATCAT GCCTGGAACC 
ATTAAAGATA ACATCATCTT TGGTGTTTCC TATGATGAAT ATAGATATAG AAGTGTCATC 
AAAGCATGCC AACTAGAAGA GGACATCTCC AAGTTTGCAG AGAAAGACAA CGTAGTTCTC 
GGAGAAGGTG GAATCACACT GAGTGGAGGT CAGCGAGCGA GAATTTCTTT AGCAAGAGCA 
GTATACAAAG ATGCTGATTT GTACCTATTA GACTCTCCTT TTGGATATCT AGATGTTTTA 
ACAGAGAAAG AAATATTTGA AAGCTGTATC TGTAAATTGA TGGCTAACAA AACTAGGATT 
TTGGTCACTT CTAAAATGGA GCACTTAAAG AAAGCTGACA AAATATTAAT TTTACATGAA 
GGTAGCATCT ATTTTTATGG GACATTTTCT GAATTACAAA ATCAGCGGCC CGACTTCAGC 
TCGAAGCTCA TGGGATGTGA CACTTTCGAC CAGTTTACTG CAGAACGAAG AAATTCAATC 
ATAACTGAGA CTTTACGGCG TTTCTCATTA GAAGGAGATA CTTCTGTGTC CTGGAATGAA 
ACGAAAAAGC CTTCTTTTAA ACAGACTGGA GAGTTTGGTG AAAAAAGGAA GAACTCCATT 
CTCAGTTCAA TCAACTCTAT AAGGAAATTT TCAGTTGTAC AAAAGACTTC ATTACAAATG 
AATGGTATTG AAGGAGCTGC TGATGCGCCT TTAGAGAGAA GACTATCCTT AGTTCCCCAT 
TCTGAACCTG GAGAGGGGAT CCTGCCTCGG AGCAATGCAG TCAACAGTGG CCCCACATTT 
CTGGGAGGGA GGAGGCAGTC CGTTCTGAAC CTTATGACCG GCTCCTCAGT GAACCAAGGT 
CAAAGCATTC ATCGAAAGAC AGCGACATCC ACACGAAAAA TGTCACTGGC TCCTCAGGCA 
AGCTTAGCCG AAATAGATAT ATATTCAAGA CGGTTATCTC AAGATACTGG CTTGGAAATA 
AGTGAAGAAA TTAATGAAGA AGATTTAAGG GATTGCTTTT TTGATGATGT GGAGAACATA 
CCAGCAGTGA CTACATGGAA CACATACCTT CGATATATTA CTGTCCACAA GAGCTTAATG 
TTTGTGCTAA TTTGGTGCTT AGTCGTTTTT CTGGTTGAGG TGGCCGCTTC TTTGGTTGTG 
TTGTGCCTAT TTCCGAAAAT ATTTTTTCAA GACAAAGGAA ACAGTACTAA GAGTGCAAAT 
AACAGTTATG CAGTGATCAT CACCAGCACC AGCTCGTATT ATATTTTTTA CATTTATGTG 
GGAGTGGCTG ACACTTTGCT TGCTCTAGGA CTCTTCAGAG GTTTACCACT GGTGCATACT 
CTAATCACAG TGTCAAAAAC TTTACACCAC AAAATGCTAC AGTCTGTTCT TCAAGCCCCT 
ATGTCAACCC TCAACACGTT GAAAACAGGT GGGATTCTTA ATAGATTCTC CAAAGATATA 
GCAGTTTTGG ATGATCTTCT GCCTCTTACC ATATTTGATT TTGTTCAGTT GTTATTAATT 
GTGATCGGAG CTGTGGTGGT CGTCTCAGTT TTACAGCCCT ACATCTTCCT AGCTACAGTG 
CCAGTGATAG CGGCTTTTAT TCTCTTGAGA GCCTACTTCC TCCACACCTC CCAGCAACTC 
AAGCAGCTGG AATCTGAAGG CAGGAGTCCA ATTTTCACTC ATCTTGTTAC AAGTTTAAAA 
GGACTATGGA CACTTCGTGC CTTGGACCGG CAGCCTTACT TTGAAACTTT ATTCCACAAA 
GCTCTGAATT TACATACTGC CAACTGGTTC TTGTATCTGT CAACACTGCG CTGGTTCCAA 
ATGAGAATAG AAATGATTTT TGTCATCTTC TTCATTGCTG TTACCTTCAT TTCCATTTTA 
ACAACAGGTG AAGGAGAAGG AAGAGTTGGG ATTATTCTAA CTTTAGCCAT GAATATCATG 
GGTACATTGC AGTGGGCTGT AAACTCTAGC ATAGATGTGG ATAGCTTGAT GCGATCTGTG 
AGCCGAGTCT TTAAGTTTAT TGATATGCCA ACAGAAGATG GTAAACCTAA CAATTCATTC 
AGACCATCCA AGGATAGTCA GCCCTCAAAA GTTATGATCA TTGAGAATCA ACATGTGAAG 
AAAGATGACA TCTGGCCCTC AGGGGGCCAA ATGACTGTCA AAGACCTCAC AGCAAAGTAT 
ACAGATGGAG GGAATGCCAT ACTAGAGAAC ATATCCTTCT CAATAAGCTC CGGCCAGAGG 
GTGGGCCTCT TGGGAAGAAC TGGATCAGGG AAGAGCACTT TGTTACTGGC TTTTTTGAGA 
CTGCTGAATA CCAAAGGAGA AATCCAAATA GATGGTGTGT CTTGGGATTC AATTACTTTG 
CAACAATGGA GGAAGGCCTT TGGAGTCATA CCACAGAAAG TATTCATCTT TTCTGGAACA 
TTTAGAAAAA ACTTGGACCC CTATGGACAG TGGAGTGATC AAGAAATATG GAAAGTTGCA 
GATGAGGTCG GACTCAGAGC TGTGATAGAG CAGTTTCCTG GGAAGCTTGA TTTTGTCCTT 
GTGGATGGGG GTTGTGTTCT AAGCCACGGC CACAAGCAGT TGATGTGCTT GGCCAGATCT 
GTTCTCAGTA AAGCAAAGAT CTTGCTGCTT GATGAACCCA GTGCTCATTT GGATCCAATA 
ACATACCAGA TCATTCGAAG AACCCTAAAA CAAGCATTTG CTAATTGCAC AGTAATCCTC 
TCTGAACACA GGATAGAAGC AATGTTGGAA TGTCAACGAT TTTTTGTCAT AGAGGAGAAC 
AAGGTGCGGC AGTATGATTC CATCCAGAGG ATGCTGAGCG AGAAGAGCCT CTTCCGGCAA 
GCCATCAGCC CCGCAGACCG CTTGAAGCTC TTGCCCCACC GGAACTCGAG CAGGCAGAGG 
TCTCGGTCCA ACATCGCGGC TCTGAAGGAG GAGACAGAAG AAGAGGTGCA AGAAACAAAG 
CTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776443.1
CDS	61..4506
Translation	MQRSPLEKASVVSKVFFSWTRPILKKGYRQRLELSDIYHISSSDSADNLSEKLEREWDRELASKKNPKLINALRRCFFWRFMFYGIILYLGEVTKAVQPLLLGRIIASYDPDNKVERSIAIYLGIGLCLLFIVRTLLLHPAIFGLHHIGMQMRIAMFSLIYKKTLKLSSRVLDKISIGRLVSLLSNNLNKFDEGLALAHFVWIAPLQVTLLMGLLWELLQAFTFCGLAFLIVLALLQAGLGKMMMKYRDQRAGKINERVVITSEMIENIQSVKAYCWEEAMEKIIENLRQTELKLTRKAAYVRYLNSSAFFFSGFFVVFLSVLPYALLKGIILRKIFTTISFCIVLRMAVTRQFPWAVQTWYDSLGAINKIQDFLQKQEYKTLEYNLTTTDVVMDNVTAFWEEGFSKLFEKAKENNNNRKISNGDNSLFFSNLLLGTPVLKDISFKIERGQLLAVAGSTGAGKTSLLLMIMGELEASEGKIKHSGRISFCSQYSWIMPGTIKDNIIFGVSYDEYRYRSVIKACQLEEDISKFAEKDNVVLGEGGITLSGGQRARISLARAVYKDADLYLLDSPFGYLDVLTEKEIFESCICKLMANKTRILVTSKMEHLKKADKILILHEGSIYFYGTFSELQNQRPDFSSKLMGCDTFDQFTAERRNSIITETLRRFSLEGDTSVSWNETKKPSFKQTGEFGEKRKNSILSSINSIRKFSVVQKTSLQMNGIEGAADAPLERRLSLVPHSEPGEGILPRSNAVNSGPTFLGGRRQSVLNLMTGSSVNQGQSIHRKTATSTRKMSLAPQASLAEIDIYSRRLSQDTGLEISEEINEEDLRDCFFDDVENIPAVTTWNTYLRYITVHKSLMFVLIWCLVVFLVEVAASLVVLCLFPKIFFQDKGNSTKSANNSYAVIITSTSSYYIFYIYVGVADTLLALGLFRGLPLVHTLITVSKTLHHKMLQSVLQAPMSTLNTLKTGGILNRFSKDIAVLDDLLPLTIFDFVQLLLIVIGAVVVVSVLQPYIFLATVPVIAAFILLRAYFLHTSQQLKQLESEGRSPIFTHLVTSLKGLWTLRALDRQPYFETLFHKALNLHTANWFLYLSTLRWFQMRIEMIFVIFFIAVTFISILTTGEGEGRVGIILTLAMNIMGTLQWAVNSSIDVDSLMRSVSRVFKFIDMPTEDGKPNNSFRPSKDSQPSKVMIIENQHVKKDDIWPSGGQMTVKDLTAKYTDGGNAILENISFSISSGQRVGLLGRTGSGKSTLLLAFLRLLNTKGEIQIDGVSWDSITLQQWRKAFGVIPQKVFIFSGTFRKNLDPYGQWSDQEIWKVADEVGLRAVIEQFPGKLDFVLVDGGCVLSHGHKQLMCLARSVLSKAKILLLDEPSAHLDPITYQIIRRTLKQAFANCTVILSEHRIEAMLECQRFFVIEENKVRQYDSIQRMLSEKSLFRQAISPADRLKLLPHRNSSRQRSRSNIAALKEETEEEVQETKL

Target ORF information:

RefSeq Version	NM_174018.2
Organism	Bos taurus(cattle)
Definition	Bos taurus CF transmembrane conductance regulator (CFTR), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174018.2

ORF Insert Sequence:

ATGCAGAGGT CGCCTCTGGA AAAGGCCAGC GTCGTCTCCA AAGTTTTTTT CAGCTGGACC 
AGACCAATTT TGAAGAAAGG ATACAGACAG CGCCTGGAAT TGTCAGACAT ATACCATATC 
TCTTCTTCTG ATTCTGCTGA CAATCTATCT GAAAAATTGG AAAGAGAATG GGACAGAGAA 
CTGGCTTCAA AGAAAAATCC CAAACTCATA AATGCCCTTC GACGATGCTT TTTCTGGAGA 
TTTATGTTCT ATGGAATCAT ATTATATTTA GGGGAAGTCA CCAAAGCAGT CCAGCCTCTC 
TTACTGGGAA GAATCATAGC TTCCTATGAC CCAGATAACA AGGTGGAACG CTCCATTGCC 
ATTTACTTAG GCATAGGCCT ATGCCTTCTC TTTATCGTGA GGACACTGCT CCTGCACCCA 
GCCATTTTTG GTCTCCATCA CATTGGAATG CAGATGAGAA TAGCTATGTT TAGTTTGATT 
TATAAGAAGA CTTTGAAGCT ATCAAGCCGT GTTCTGGATA AAATAAGTAT TGGACGACTT 
GTTAGTCTCC TTTCCAACAA CCTGAACAAA TTTGATGAAG GACTCGCACT GGCACATTTT 
GTGTGGATCG CTCCTCTGCA AGTGACGCTG CTCATGGGGC TGCTCTGGGA GTTGTTGCAG 
GCCTTCACCT TCTGTGGGCT TGCTTTCCTC ATAGTCCTCG CCCTTCTTCA AGCCGGATTA 
GGGAAAATGA TGATGAAGTA CAGAGATCAG AGAGCTGGAA AGATCAATGA AAGAGTGGTG 
ATAACGTCAG AAATGATTGA AAATATCCAA TCAGTTAAGG CATACTGCTG GGAAGAAGCA 
ATGGAAAAAA TCATTGAAAA CCTAAGACAA ACAGAACTGA AACTCACCCG GAAGGCAGCC 
TATGTGAGAT ACCTCAATAG CTCAGCCTTC TTCTTCTCAG GGTTCTTTGT GGTATTTTTA 
TCTGTGCTTC CTTATGCACT GCTCAAAGGA ATCATCCTTC GAAAAATATT CACAACCATC 
TCATTCTGCA TCGTTCTGCG CATGGCAGTT ACTCGGCAGT TCCCCTGGGC TGTACAAACT 
TGGTATGATT CTCTGGGAGC AATAAACAAA ATACAGGATT TCTTACAAAA GCAAGAATAT 
AAGACATTGG AATATAACTT AACAACTACA GATGTAGTGA TGGATAATGT AACAGCCTTC 
TGGGAGGAGG GATTTAGTAA ATTATTTGAG AAAGCAAAAG AAAATAATAA CAATAGAAAA 
ATTTCCAATG GTGATAACAG CCTCTTCTTC AGTAACTTAC TTCTTGGTAC TCCTGTCCTG 
AAAGATATCA GTTTCAAGAT AGAAAGAGGA CAGTTGTTGG CAGTTGCTGG ATCTACGGGA 
GCAGGCAAGA CTTCACTTCT CTTGATGATT ATGGGAGAAT TGGAAGCTTC AGAGGGTAAA 
ATTAAGCACA GTGGAAGAAT TTCATTCTGC TCTCAGTATT CCTGGATCAT GCCTGGAACC 
ATTAAAGATA ACATCATCTT TGGTGTTTCC TATGATGAAT ATAGATATAG AAGTGTCATC 
AAAGCATGCC AACTAGAAGA GGACATCTCC AAGTTTGCAG AGAAAGACAA CGTAGTTCTC 
GGAGAAGGTG GAATCACACT GAGTGGAGGT CAGCGAGCGA GAATTTCTTT AGCAAGAGCA 
GTATACAAAG ATGCTGATTT GTACCTATTA GACTCTCCTT TTGGATATCT AGATGTTTTA 
ACAGAGAAAG AAATATTTGA AAGCTGTATC TGTAAATTGA TGGCTAACAA AACTAGGATT 
TTGGTCACTT CTAAAATGGA GCACTTAAAG AAAGCTGACA AAATATTAAT TTTACATGAA 
GGTAGCATCT ATTTTTATGG GACATTTTCT GAATTACAAA ATCAGCGGCC CGACTTCAGC 
TCGAAGCTCA TGGGATGTGA CACTTTCGAC CAGTTTACTG CAGAACGAAG AAATTCAATC 
ATAACTGAGA CTTTACGGCG TTTCTCATTA GAAGGAGATA CTTCTGTGTC CTGGAATGAA 
ACGAAAAAGC CTTCTTTTAA ACAGACTGGA GAGTTTGGTG AAAAAAGGAA GAACTCCATT 
CTCAGTTCAA TCAACTCTAT AAGGAAATTT TCAGTTGTAC AAAAGACTTC ATTACAAATG 
AATGGTATTG AAGGAGCTGC TGATGCGCCT TTAGAGAGAA GACTATCCTT AGTTCCCCAT 
TCTGAACCTG GAGAGGGGAT CCTGCCTCGG AGCAATGCAG TCAACAGTGG CCCCACATTT 
CTGGGAGGGA GGAGGCAGTC CGTTCTGAAC CTTATGACCG GCTCCTCAGT GAACCAAGGT 
CAAAGCATTC ATCGAAAGAC AGCGACATCC ACACGAAAAA TGTCACTGGC TCCTCAGGCA 
AGCTTAGCCG AAATAGATAT ATATTCAAGA CGGTTATCTC AAGATACTGG CTTGGAAATA 
AGTGAAGAAA TTAATGAAGA AGATTTAAGG GATTGCTTTT TTGATGATGT GGAGAACATA 
CCAGCAGTGA CTACATGGAA CACATACCTT CGATATATTA CTGTCCACAA GAGCTTAATG 
TTTGTGCTAA TTTGGTGCTT AGTCGTTTTT CTGGTTGAGG TGGCCGCTTC TTTGGTTGTG 
TTGTGCCTAT TTCCGAAAAT ATTTTTTCAA GACAAAGGAA ACAGTACTAA GAGTGCAAAT 
AACAGTTATG CAGTGATCAT CACCAGCACC AGCTCGTATT ATATTTTTTA CATTTATGTG 
GGAGTGGCTG ACACTTTGCT TGCTCTAGGA CTCTTCAGAG GTTTACCACT GGTGCATACT 
CTAATCACAG TGTCAAAAAC TTTACACCAC AAAATGCTAC AGTCTGTTCT TCAAGCCCCT 
ATGTCAACCC TCAACACGTT GAAAACAGGT GGGATTCTTA ATAGATTCTC CAAAGATATA 
GCAGTTTTGG ATGATCTTCT GCCTCTTACC ATATTTGATT TTGTTCAGTT GTTATTAATT 
GTGATCGGAG CTGTGGTGGT CGTCTCAGTT TTACAGCCCT ACATCTTCCT AGCTACAGTG 
CCAGTGATAG CGGCTTTTAT TCTCTTGAGA GCCTACTTCC TCCACACCTC CCAGCAACTC 
AAGCAGCTGG AATCTGAAGG CAGGAGTCCA ATTTTCACTC ATCTTGTTAC AAGTTTAAAA 
GGACTATGGA CACTTCGTGC CTTGGACCGG CAGCCTTACT TTGAAACTTT ATTCCACAAA 
GCTCTGAATT TACATACTGC CAACTGGTTC TTGTATCTGT CAACACTGCG CTGGTTCCAA 
ATGAGAATAG AAATGATTTT TGTCATCTTC TTCATTGCTG TTACCTTCAT TTCCATTTTA 
ACAACAGGTG AAGGAGAAGG AAGAGTTGGG ATTATTCTAA CTTTAGCCAT GAATATCATG 
GGTACATTGC AGTGGGCTGT AAACTCTAGC ATAGATGTGG ATAGCTTGAT GCGATCTGTG 
AGCCGAGTCT TTAAGTTTAT TGATATGCCA ACAGAAGATG GTAAACCTAA CAATTCATTC 
AGACCATCCA AGGATAGTCA GCCCTCAAAA GTTATGATCA TTGAGAATCA ACATGTGAAG 
AAAGATGACA TCTGGCCCTC AGGGGGCCAA ATGACTGTCA AAGACCTCAC AGCAAAGTAT 
ACAGATGGAG GGAATGCCAT ACTAGAGAAC ATATCCTTCT CAATAAGCTC CGGCCAGAGG 
GTGGGCCTCT TGGGAAGAAC TGGATCAGGG AAGAGCACTT TGTTACTGGC TTTTTTGAGA 
CTGCTGAATA CCAAAGGAGA AATCCAAATA GATGGTGTGT CTTGGGATTC AATTACTTTG 
CAACAATGGA GGAAGGCCTT TGGAGTCATA CCACAGAAAG TATTCATCTT TTCTGGAACA 
TTTAGAAAAA ACTTGGACCC CTATGGACAG TGGAGTGATC AAGAAATATG GAAAGTTGCA 
GATGAGGTCG GACTCAGAGC TGTGATAGAG CAGTTTCCTG GGAAGCTTGA TTTTGTCCTT 
GTGGATGGGG GTTGTGTTCT AAGCCACGGC CACAAGCAGT TGATGTGCTT GGCCAGATCT 
GTTCTCAGTA AAGCAAAGAT CTTGCTGCTT GATGAACCCA GTGCTCATTT GGATCCAATA 
ACATACCAGA TCATTCGAAG AACCCTAAAA CAAGCATTTG CTAATTGCAC AGTAATCCTC 
TCTGAACACA GGATAGAAGC AATGTTGGAA TGTCAACGAT TTTTTGTCAT AGAGGAGAAC 
AAGGTGCGGC AGTATGATTC CATCCAGAGG ATGCTGAGCG AGAAGAGCCT CTTCCGGCAA 
GCCATCAGCC CCGCAGACCG CTTGAAGCTC TTGCCCCACC GGAACTCGAG CAGGCAGAGG 
TCTCGGTCCA ACATCGCGGC TCTGAAGGAG GAGACAGAAG AAGAGGTGCA AGAAACAAAG 
CTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Missense mutation within cystic fibrosis transmembrane conductance regulator (CFTR) gene is associated with selected parameters of the frozen-thawed sperm in Holstein-Friesian bulls.
Polish journal of veterinary sciences22(2)221-225(2019 Jun)
Kaminski S,Hering DM,Kordan W,Lecewicz M

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Marçais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

A comparative genomic analysis of the cow, pig, and human CFTR genes identifies potential intronic regulatory elements.
Genomics81(6)628-39(2003 Jun)
Williams SH,Mouchel N,Harris A

Phosphorylation of protein kinase C sites in NBD1 and the R domain control CFTR channel activation by PKA.
The Journal of physiology548(Pt 1)39-52(2003 Apr)
Chappe V,Hinkson DA,Zhu T,Chang XB,Riordan JR,Hanrahan JW

Estimate of nucleotide diversity in dogs with a pool-and-sequence method.
Mammalian genome : official journal of the International Mammalian Genome Society11(12)1079-86(2000 Dec)
Brouillette JA,Andrew JR,Venta PJ

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CFTR cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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