cftr cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	cftr
Entrez Gene ID	559080
Full Name	cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7)
Synonyms	si:dkey-270i2.2
General protein information	Preferred Names cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7) Names cystic fibrosis transmembrane conductance regulator ATP-binding cassette sub-family C member 7 abcc7 cAMP-dependent chloride channel channel conductance-controlling ATPase
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001044883.1	NP_001038348.1	cystic fibrosis transmembrane conductance regulator

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	CFTR	NP_001028110.1
Pan troglodytes (chimpanzee)	CFTR	NP_001073386.1
Rattus norvegicus (Norway rat)	Cftr	NP_113694.1
Canis lupus familiaris (dog)	CFTR	NP_001007144.1
Mus musculus (house mouse)	Cftr	NP_066388.1
Xenopus tropicalis (tropical clawed frog)	cftr	XP_002935002.2
Homo sapiens (human)	CFTR	NP_000483.3
Bos taurus (cattle)	CFTR	NP_776443.1
Danio rerio (zebrafish)	cftr	NP_001038348.1
Gallus gallus (chicken)	CFTR	NP_001099136.1

Gene Ontology
Bibliography

Related articles in PubMed

Role of zebrafish ClC-K/barttin channels in apical kidney chloride reabsorption.
Pérez-Rius C, Castellanos A, Gaitán-Peñas H, Navarro A, Artuch R, Barrallo-Gimeno A, Estévez R
The Journal of physiology597(15)3969-3983(2019 Aug)

CFTR Protects against Mycobacterium abscessus Infection by Fine-Tuning Host Oxidative Defenses.
Bernut A, Dupont C, Ogryzko NV, Neyret A, Herrmann JL, Floto RA, Renshaw SA, Kremer L
Cell reports26(7)1828-1840.e4(2019 02)

CFTR is required for the migration of primordial germ cells during zebrafish early embryogenesis.
Liao H, Chen Y, Li Y, Xue S, Liu M, Lin Z, Liu Y, Chan HC, Zhang X, Sun H
Reproduction (Cambridge, England)156(3)261-268(2018 09)

Structure of Transmembrane Helix 8 and Possible Membrane Defects in CFTR.
Corradi V, Gu RX, Vergani P, Tieleman DP
Biophysical journal114(8)1751-1754(2018 04)

Functional characterization reveals that zebrafish CFTR prefers to occupy closed channel conformations.
Zhang J, Yu YC, Yeh JT, Hwang TC
PloS one13(12)e0209862(2018)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following cftr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cftr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa03837	NM_001044883.1 Latest version!	Danio rerio CF transmembrane conductance regulator (cftr), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cftr ( NM_001044883.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001038348.1 Danio rerio CF transmembrane conductance regulator (cftr), mRNA.

Price & Availability↑

CloneID	ODa03837
Clone ID Related Accession (Same CDS sequence)	NM_001044883.1
Accession Version	NM_001044883.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4458bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576771200000
Organism	Danio rerio(zebrafish)
Product	cystic fibrosis transmembrane conductance regulator
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX470130.4. On Sep 1, 2006 this sequence version replaced XM_682387.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC171654.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGCAGAGAT CACCTGTGGA GGATGCCAAC TGCCTCTCCA GATACTTCTT TTGGTGGACA 
AACCCTATTA TGAGAAAGGG TTTTAAGGAG AAACTGAGGC CATCCGATGT TTATCAAGCA 
CCCAGTCAAG ATGCAGCAGA CATCCTCGCA GAGCGCTTGG AAAAGGAATG GGACAGAGAA 
GTTGCATCTG GAAAGAAAAA ACCCAGTCTC CTGAGAGCGA TGGCACGATG CTACATAAAA 
CCTTTTCTGT TGTTTGGCTT CCTGCTGTAT ATTGGTGAAG CTACAAAAAC AGTGCAACCG 
CAGCTTTTAG GACGGATCAT TGCATCATTT GACCCGGCCC ATGAGCCGGA GCGAGCCAAT 
GGATACTTTC TTGCCTTTGG TCTTGGTTTG TTGTTTACTG CCCGCTTCCT CCTGCTCCAG 
CCTGCCATGT TTGGGCTGCA CCACCTGGGC ATGCAAATCA GGATTGCACT CTTTAGCATA 
ATATATAAAA AGACCTTGAA ACTTTCCAGC CGTGTCTTGG ACAAGATCAG CACAGGTCAG 
CTGGTCAGTC TGATGTCGGC CAACCTGGGC AAGTTTGATC AGAGCCTGGG TATGGCCCAT 
TTTATATGGA TATCACCACT GCAATGCATC CTGTGTACAG GGCTTATCTG GGAACTCATT 
GATGTCAATA GCTTCTGTGC ACTTGCCGCC ATCTCTTTAC TGGGTGTTTT GCAGGCCTTC 
CTTTCACATA AAATGGGTCC TTACAAAGCA CAGAAGGTGC TGTTGACCAA CAAACGGCTG 
GCTCTGACCT CAGAGATCAT GGAGAACCTG CATTCGGTAA AGGCGTATGG CTGGGAGGAG 
ATAATGGAGA CACTCATCAA GAACATCAGA CAGGATGAGG TGAAGCTGAC CAGGAAGATC 
GGCTCGCTGC GCTACTTCTA CAGCTCGGCG TACTTTTTCT CGGCCATCTT TGTGATTGTG 
GCTGCAGTGG TGCCTCATGC TCTGAGTCGG GGAATCAACC TGCGCCGCAT TTTCACCACT 
CTCTCCTACT GTATGGTGCT GCGGATGACA GTCACCCGAC AGCTGCCCGG CTCCATCCAG 
ATGTGGTACG ACACCATGCG GCTCATCTGG AAGATTGAAG AATTTCTTAG TAAAGAAGAA 
TACAAGTTAA TGGAGTACGA TCTCAGCATC ACAGAGCTGG AGCTTCAAGA TGTGACTGCT 
TCATGGGATG AGGGTCCCGG TGAACTTTTG GAAAGGATAA AACAGGAGAA CAAAGCTAAT 
GGGCATCACA ATGGAGACGC AGGCCTCTTC TTCACTAACC TGTATGTGGC TCCTGTACTG 
AAGGACATCA GTCTGAAGCT GAAGAAAGGA GAGATGTTGG CTGTTACTGG TTCTATGGGA 
TCTGGGAAAA GCTCATTGTT GATGACCATC CTCGGGGAGC TGGTTCCCTC CTCAGGGAAG 
ATCCGACACA GCGGTCGAAT CTCTTACTCC TCCCAAACAG CCTGGATCAT GCCTGGAACC 
ATCCGAGACA ACATCCTCTT TGGCCTGACC TACGACGAGT ATCGCTACAA GAGTGTGGTG 
AAAGCGTGCC AGCTTGAGGA GGATCTGGCT GCTCTTCCAG AGAAGGATAA GACACCTATG 
GCTGAGGGCG GTTTAAACTT GAGCGGGGGT CAGAAGGCAC GCGTGGCTCT GGCCAGGGCT 
GTTTACAGAG ATGCTGATCT TTACCTACTT GACGCACCAT TCACTCATCT GGATATTGCA 
ACAGAAAAGG AAATATTTGA CAAATGCCTC TGTAAACTGA TGGCCTCAAA GACACGCATC 
TTGGTCACAA ATAAGATTGA ACATTTGAAG CGGGCTGATA AGATCCTACT GCTCCACAAC 
GGTGAAAGCT TTTTCTACGG CACCTTTCCT GAGCTTCAGT CAGAGCGGCC GGATTTCAGC 
TCTTTGCTTC TAGGTTTGGA GGCCTACGAC AATATCAGTG CCGAACGTCG CTGCTCCATC 
CTTACCGAGA CCCTCCATCG CGTCTCAGTA GATGAAAGCG CCGGCATGCA ACCTGAACGC 
TCGGCTTTTC GTCAGGTGCC TCCAACCAAG CCCATGTACA TCGACGAAAG AAAAGCTTCA 
GTAATCGTCA ACCCTCTTGG GGTGGCTCGT AAGGCATCTT TTATTCAGGT CCCAGAGGAA 
GAGGTGCGGA GGACATTACC TGACAGAAAG TTCTCCCTGG TGCCTGAGAA CGAGTTAGTG 
GATGAGTCCT TCATGGGGAG CGATGTGTAT CATAACCATG GAGTGCATAT GGCTGGTCAG 
AGGAGGCAGT CGGTGTTAGC CTTTATGACC AATGCACAGG GCCAGGGTCG TCGCGAGCAC 
CTCCAGTCAT CTTTCCGCAG ACGTCTTTCT GTCGTGCCTC AGAGTGAACT GGCATCTGAG 
CTGGACATCT ACACTCGCCG ACTATCGGAC AGCACGTATG ACATGACTGG CATCCTGGAG 
GAAGAAAACA TTGAGGCATG CTTAACTGAT GAAATTGATG AAATAGAAGA AACATTCGAG 
ACGACAAAGT GGAACACATA CGTCAGATAT GTGTCAAATA ATAAAAGCCT TCTTTATGTT 
CTGATCTTCA TCCTCTTTAT TGCTGCAATT GAGATTGCAG GATCTGTAGC AGGGATTTTT 
CTGATAACCG ATGAACTTTG GAGAGAGGAA CATCAACGAT CTGAGCCAAA CATGACAAAG 
CACTCCAACG CGTCTTCATC TGGACAAACA TATGCCATCA CTGTGACTCC CACAAGCAGC 
TATTACATTC TCTACATATA TGTGGCCACG TCAGAGAGCC TCCTCGCGAT GGGATTTTTC 
AGAGGTCTTC CGTTTGTACA CACAACCATC ACCATCTCCA AAAAACTGCA CCAGAAGATG 
CTACATGCTG TTCTCAGTGC TCCCATGTCT GTACTCAACA CTATGAAGAC AGGCCGAATC 
ATGAATAGAT TCACCAAAGA TATGGCCACA ATAGACGACA TGCTTCCTCT CCTCATGTTT 
GACTTTGTCC AGCTGACAGT AGTGGTGGTT GGATGTATTC TTGTGGTGTC CATTGTGCGG 
CCGTATATAT TCCTCGCTGC TACTCCCCTG GCCATTATTT TCATTGTGAT GAGGAAGTAC 
TTTCTCCGCA CGGGTCAACA GCTTAAACAG CTGGAGACAG AAGCCCGGAG CCCCATTTTC 
TCGCACCTCA TCATGTCTCT GAAGGGTTTG TGGACCATCA GGGCTTTCGA GCGACAGGCC 
TACTTCGAGG CCCTTTTCCA CAAGACACTC AACACCCACA CGGCCACCTG GTTCCTCTAC 
TTGTCCACTC TCCGATGGTT CCTCTTCCGC GCCGACATCC TCTTCGTCTT CTTTTTCACG 
CTGGCCGCCT GGATAGCGGT AGGAACCAAC CAGGATAAAC CGGGCGAGAT TGGTATCATC 
ATATGCCTGG CAATGCTCAT TCTCGGCACC TTTCAGTGGT GTGTTGCCAC CAGCATTGCA 
GTGGATGGAA TGATGCGTTC GGTTGACCGG GTGTTTAAGT TCATTGACCT TCCATCTGAG 
ACGCCCAAAC CAGACAAAGG CAAAGACTCA GATCTGATTA TTGAGAACGT CGATGCACAG 
GCTGACTCGA GCTGGCCACA CCGCGGTCAG ATCGAAGTGA GGAATTTAAC AGTCAAATAC 
ACAGAAGCGG GTCACGCTGT CCTCAAAAAC CTGTCCTTCA GTGCGGAGGG GAGACAGAGG 
GTGGGCATCT TGGGCCGAAC AGGTTCAGGG AAGAGTTCTC TGTTCAATGC CCTCCTGAAG 
CTGGTTTATA CTGATGGCGA AATCTCCATC GACGGGGTCA ACTGGAACAA AATGCCGCTT 
CAGAAATGGA GGAAAGCCTT TGGTGTAGTG CCTCAGAAAG TGTTCATCTT TACGGGACCG 
TTACGCATGA ACCTGGACCC TTATGGCTGT CACAGTGATG AAGAGCTGTG GCGGGTCGCT 
GAGGAGGTGG GTCTGAAGAC AGTGATTGAG CAGTTTCCAG ACAAACTGGA CTTCCAGCTG 
GAGTACGGCG GTTATGTGCT GAGTAACGGC CACAAACAGC TCATATGCCT GGCTCGCTCT 
ATCCTCAGCG GAGCCCGGAT CCTCCTCCTG GATGAGCCCA GCGCTCACCT CGACCCTGTA 
ACAATAAAGG TGCTGAAGAA GACACTGAGA CAGTCTTTCT CCACATGCAC TATATTACTG 
TCTGAACACA AAGTGGAGCC GCTGCTCGAG TGCCAGTCTT TCCTGATGAT GGACAAGGGT 
CAGGTGAAGA CCTACGACTC CATCCAAAAG CTTCTGAATG AGACCAGTCA CCTAAAGCAG 
GCCATCAGTC CAGCCGAGCG GCTTAAACTC TTCCCTCGCC GAAACTCCAG CATGAGGACG 
CCTCAATCCA AACTGAGCTC AGTCACGCAG ACCCTGCAAG AGGAGGCAGA GGACAACATC 
CAGGACACTC GCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001038348.1
CDS	1..4458
Translation	MQRSPVEDANCLSRYFFWWTNPIMRKGFKEKLRPSDVYQAPSQDAADILAERLEKEWDREVASGKKKPSLLRAMARCYIKPFLLFGFLLYIGEATKTVQPQLLGRIIASFDPAHEPERANGYFLAFGLGLLFTARFLLLQPAMFGLHHLGMQIRIALFSIIYKKTLKLSSRVLDKISTGQLVSLMSANLGKFDQSLGMAHFIWISPLQCILCTGLIWELIDVNSFCALAAISLLGVLQAFLSHKMGPYKAQKVLLTNKRLALTSEIMENLHSVKAYGWEEIMETLIKNIRQDEVKLTRKIGSLRYFYSSAYFFSAIFVIVAAVVPHALSRGINLRRIFTTLSYCMVLRMTVTRQLPGSIQMWYDTMRLIWKIEEFLSKEEYKLMEYDLSITELELQDVTASWDEGPGELLERIKQENKANGHHNGDAGLFFTNLYVAPVLKDISLKLKKGEMLAVTGSMGSGKSSLLMTILGELVPSSGKIRHSGRISYSSQTAWIMPGTIRDNILFGLTYDEYRYKSVVKACQLEEDLAALPEKDKTPMAEGGLNLSGGQKARVALARAVYRDADLYLLDAPFTHLDIATEKEIFDKCLCKLMASKTRILVTNKIEHLKRADKILLLHNGESFFYGTFPELQSERPDFSSLLLGLEAYDNISAERRCSILTETLHRVSVDESAGMQPERSAFRQVPPTKPMYIDERKASVIVNPLGVARKASFIQVPEEEVRRTLPDRKFSLVPENELVDESFMGSDVYHNHGVHMAGQRRQSVLAFMTNAQGQGRREHLQSSFRRRLSVVPQSELASELDIYTRRLSDSTYDMTGILEEENIEACLTDEIDEIEETFETTKWNTYVRYVSNNKSLLYVLIFILFIAAIEIAGSVAGIFLITDELWREEHQRSEPNMTKHSNASSSGQTYAITVTPTSSYYILYIYVATSESLLAMGFFRGLPFVHTTITISKKLHQKMLHAVLSAPMSVLNTMKTGRIMNRFTKDMATIDDMLPLLMFDFVQLTVVVVGCILVVSIVRPYIFLAATPLAIIFIVMRKYFLRTGQQLKQLETEARSPIFSHLIMSLKGLWTIRAFERQAYFEALFHKTLNTHTATWFLYLSTLRWFLFRADILFVFFFTLAAWIAVGTNQDKPGEIGIIICLAMLILGTFQWCVATSIAVDGMMRSVDRVFKFIDLPSETPKPDKGKDSDLIIENVDAQADSSWPHRGQIEVRNLTVKYTEAGHAVLKNLSFSAEGRQRVGILGRTGSGKSSLFNALLKLVYTDGEISIDGVNWNKMPLQKWRKAFGVVPQKVFIFTGPLRMNLDPYGCHSDEELWRVAEEVGLKTVIEQFPDKLDFQLEYGGYVLSNGHKQLICLARSILSGARILLLDEPSAHLDPVTIKVLKKTLRQSFSTCTILLSEHKVEPLLECQSFLMMDKGQVKTYDSIQKLLNETSHLKQAISPAERLKLFPRRNSSMRTPQSKLSSVTQTLQEEAEDNIQDTRL

Target ORF information:

RefSeq Version	NM_001044883.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio CF transmembrane conductance regulator (cftr), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001044883.1

ORF Insert Sequence:

ATGCAGAGAT CACCTGTGGA GGATGCCAAC TGCCTCTCCA GATACTTCTT TTGGTGGACA 
AACCCTATTA TGAGAAAGGG TTTTAAGGAG AAACTGAGGC CATCCGATGT TTATCAAGCA 
CCCAGTCAAG ATGCAGCAGA CATCCTCGCA GAGCGCTTGG AAAAGGAATG GGACAGAGAA 
GTTGCATCTG GAAAGAAAAA ACCCAGTCTC CTGAGAGCGA TGGCACGATG CTACATAAAA 
CCTTTTCTGT TGTTTGGCTT CCTGCTGTAT ATTGGTGAAG CTACAAAAAC AGTGCAACCG 
CAGCTTTTAG GACGGATCAT TGCATCATTT GACCCGGCCC ATGAGCCGGA GCGAGCCAAT 
GGATACTTTC TTGCCTTTGG TCTTGGTTTG TTGTTTACTG CCCGCTTCCT CCTGCTCCAG 
CCTGCCATGT TTGGGCTGCA CCACCTGGGC ATGCAAATCA GGATTGCACT CTTTAGCATA 
ATATATAAAA AGACCTTGAA ACTTTCCAGC CGTGTCTTGG ACAAGATCAG CACAGGTCAG 
CTGGTCAGTC TGATGTCGGC CAACCTGGGC AAGTTTGATC AGAGCCTGGG TATGGCCCAT 
TTTATATGGA TATCACCACT GCAATGCATC CTGTGTACAG GGCTTATCTG GGAACTCATT 
GATGTCAATA GCTTCTGTGC ACTTGCCGCC ATCTCTTTAC TGGGTGTTTT GCAGGCCTTC 
CTTTCACATA AAATGGGTCC TTACAAAGCA CAGAAGGTGC TGTTGACCAA CAAACGGCTG 
GCTCTGACCT CAGAGATCAT GGAGAACCTG CATTCGGTAA AGGCGTATGG CTGGGAGGAG 
ATAATGGAGA CACTCATCAA GAACATCAGA CAGGATGAGG TGAAGCTGAC CAGGAAGATC 
GGCTCGCTGC GCTACTTCTA CAGCTCGGCG TACTTTTTCT CGGCCATCTT TGTGATTGTG 
GCTGCAGTGG TGCCTCATGC TCTGAGTCGG GGAATCAACC TGCGCCGCAT TTTCACCACT 
CTCTCCTACT GTATGGTGCT GCGGATGACA GTCACCCGAC AGCTGCCCGG CTCCATCCAG 
ATGTGGTACG ACACCATGCG GCTCATCTGG AAGATTGAAG AATTTCTTAG TAAAGAAGAA 
TACAAGTTAA TGGAGTACGA TCTCAGCATC ACAGAGCTGG AGCTTCAAGA TGTGACTGCT 
TCATGGGATG AGGGTCCCGG TGAACTTTTG GAAAGGATAA AACAGGAGAA CAAAGCTAAT 
GGGCATCACA ATGGAGACGC AGGCCTCTTC TTCACTAACC TGTATGTGGC TCCTGTACTG 
AAGGACATCA GTCTGAAGCT GAAGAAAGGA GAGATGTTGG CTGTTACTGG TTCTATGGGA 
TCTGGGAAAA GCTCATTGTT GATGACCATC CTCGGGGAGC TGGTTCCCTC CTCAGGGAAG 
ATCCGACACA GCGGTCGAAT CTCTTACTCC TCCCAAACAG CCTGGATCAT GCCTGGAACC 
ATCCGAGACA ACATCCTCTT TGGCCTGACC TACGACGAGT ATCGCTACAA GAGTGTGGTG 
AAAGCGTGCC AGCTTGAGGA GGATCTGGCT GCTCTTCCAG AGAAGGATAA GACACCTATG 
GCTGAGGGCG GTTTAAACTT GAGCGGGGGT CAGAAGGCAC GCGTGGCTCT GGCCAGGGCT 
GTTTACAGAG ATGCTGATCT TTACCTACTT GACGCACCAT TCACTCATCT GGATATTGCA 
ACAGAAAAGG AAATATTTGA CAAATGCCTC TGTAAACTGA TGGCCTCAAA GACACGCATC 
TTGGTCACAA ATAAGATTGA ACATTTGAAG CGGGCTGATA AGATCCTACT GCTCCACAAC 
GGTGAAAGCT TTTTCTACGG CACCTTTCCT GAGCTTCAGT CAGAGCGGCC GGATTTCAGC 
TCTTTGCTTC TAGGTTTGGA GGCCTACGAC AATATCAGTG CCGAACGTCG CTGCTCCATC 
CTTACCGAGA CCCTCCATCG CGTCTCAGTA GATGAAAGCG CCGGCATGCA ACCTGAACGC 
TCGGCTTTTC GTCAGGTGCC TCCAACCAAG CCCATGTACA TCGACGAAAG AAAAGCTTCA 
GTAATCGTCA ACCCTCTTGG GGTGGCTCGT AAGGCATCTT TTATTCAGGT CCCAGAGGAA 
GAGGTGCGGA GGACATTACC TGACAGAAAG TTCTCCCTGG TGCCTGAGAA CGAGTTAGTG 
GATGAGTCCT TCATGGGGAG CGATGTGTAT CATAACCATG GAGTGCATAT GGCTGGTCAG 
AGGAGGCAGT CGGTGTTAGC CTTTATGACC AATGCACAGG GCCAGGGTCG TCGCGAGCAC 
CTCCAGTCAT CTTTCCGCAG ACGTCTTTCT GTCGTGCCTC AGAGTGAACT GGCATCTGAG 
CTGGACATCT ACACTCGCCG ACTATCGGAC AGCACGTATG ACATGACTGG CATCCTGGAG 
GAAGAAAACA TTGAGGCATG CTTAACTGAT GAAATTGATG AAATAGAAGA AACATTCGAG 
ACGACAAAGT GGAACACATA CGTCAGATAT GTGTCAAATA ATAAAAGCCT TCTTTATGTT 
CTGATCTTCA TCCTCTTTAT TGCTGCAATT GAGATTGCAG GATCTGTAGC AGGGATTTTT 
CTGATAACCG ATGAACTTTG GAGAGAGGAA CATCAACGAT CTGAGCCAAA CATGACAAAG 
CACTCCAACG CGTCTTCATC TGGACAAACA TATGCCATCA CTGTGACTCC CACAAGCAGC 
TATTACATTC TCTACATATA TGTGGCCACG TCAGAGAGCC TCCTCGCGAT GGGATTTTTC 
AGAGGTCTTC CGTTTGTACA CACAACCATC ACCATCTCCA AAAAACTGCA CCAGAAGATG 
CTACATGCTG TTCTCAGTGC TCCCATGTCT GTACTCAACA CTATGAAGAC AGGCCGAATC 
ATGAATAGAT TCACCAAAGA TATGGCCACA ATAGACGACA TGCTTCCTCT CCTCATGTTT 
GACTTTGTCC AGCTGACAGT AGTGGTGGTT GGATGTATTC TTGTGGTGTC CATTGTGCGG 
CCGTATATAT TCCTCGCTGC TACTCCCCTG GCCATTATTT TCATTGTGAT GAGGAAGTAC 
TTTCTCCGCA CGGGTCAACA GCTTAAACAG CTGGAGACAG AAGCCCGGAG CCCCATTTTC 
TCGCACCTCA TCATGTCTCT GAAGGGTTTG TGGACCATCA GGGCTTTCGA GCGACAGGCC 
TACTTCGAGG CCCTTTTCCA CAAGACACTC AACACCCACA CGGCCACCTG GTTCCTCTAC 
TTGTCCACTC TCCGATGGTT CCTCTTCCGC GCCGACATCC TCTTCGTCTT CTTTTTCACG 
CTGGCCGCCT GGATAGCGGT AGGAACCAAC CAGGATAAAC CGGGCGAGAT TGGTATCATC 
ATATGCCTGG CAATGCTCAT TCTCGGCACC TTTCAGTGGT GTGTTGCCAC CAGCATTGCA 
GTGGATGGAA TGATGCGTTC GGTTGACCGG GTGTTTAAGT TCATTGACCT TCCATCTGAG 
ACGCCCAAAC CAGACAAAGG CAAAGACTCA GATCTGATTA TTGAGAACGT CGATGCACAG 
GCTGACTCGA GCTGGCCACA CCGCGGTCAG ATCGAAGTGA GGAATTTAAC AGTCAAATAC 
ACAGAAGCGG GTCACGCTGT CCTCAAAAAC CTGTCCTTCA GTGCGGAGGG GAGACAGAGG 
GTGGGCATCT TGGGCCGAAC AGGTTCAGGG AAGAGTTCTC TGTTCAATGC CCTCCTGAAG 
CTGGTTTATA CTGATGGCGA AATCTCCATC GACGGGGTCA ACTGGAACAA AATGCCGCTT 
CAGAAATGGA GGAAAGCCTT TGGTGTAGTG CCTCAGAAAG TGTTCATCTT TACGGGACCG 
TTACGCATGA ACCTGGACCC TTATGGCTGT CACAGTGATG AAGAGCTGTG GCGGGTCGCT 
GAGGAGGTGG GTCTGAAGAC AGTGATTGAG CAGTTTCCAG ACAAACTGGA CTTCCAGCTG 
GAGTACGGCG GTTATGTGCT GAGTAACGGC CACAAACAGC TCATATGCCT GGCTCGCTCT 
ATCCTCAGCG GAGCCCGGAT CCTCCTCCTG GATGAGCCCA GCGCTCACCT CGACCCTGTA 
ACAATAAAGG TGCTGAAGAA GACACTGAGA CAGTCTTTCT CCACATGCAC TATATTACTG 
TCTGAACACA AAGTGGAGCC GCTGCTCGAG TGCCAGTCTT TCCTGATGAT GGACAAGGGT 
CAGGTGAAGA CCTACGACTC CATCCAAAAG CTTCTGAATG AGACCAGTCA CCTAAAGCAG 
GCCATCAGTC CAGCCGAGCG GCTTAAACTC TTCCCTCGCC GAAACTCCAG CATGAGGACG 
CCTCAATCCA AACTGAGCTC AGTCACGCAG ACCCTGCAAG AGGAGGCAGA GGACAACATC 
CAGGACACTC GCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Role of zebrafish ClC-K/barttin channels in apical kidney chloride reabsorption.
The Journal of physiology597(15)3969-3983(2019 Aug)
Pérez-Rius C,Castellanos A,Gaitán-Peñas H,Navarro A,Artuch R,Barrallo-Gimeno A,Estévez R

CFTR Protects against Mycobacterium abscessus Infection by Fine-Tuning Host Oxidative Defenses.
Cell reports26(7)1828-1840.e4(2019 02)
Bernut A,Dupont C,Ogryzko NV,Neyret A,Herrmann JL,Floto RA,Renshaw SA,Kremer L

CFTR is required for the migration of primordial germ cells during zebrafish early embryogenesis.
Reproduction (Cambridge, England)156(3)261-268(2018 09)
Liao H,Chen Y,Li Y,Xue S,Liu M,Lin Z,Liu Y,Chan HC,Zhang X,Sun H

Structure of Transmembrane Helix 8 and Possible Membrane Defects in CFTR.
Biophysical journal114(8)1751-1754(2018 04)
Corradi V,Gu RX,Vergani P,Tieleman DP

Functional characterization reveals that zebrafish CFTR prefers to occupy closed channel conformations.
PloS one13(12)e0209862(2018)
Zhang J,Yu YC,Yeh JT,Hwang TC

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cftr cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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