KIT cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	KIT
Entrez Gene ID	280832
Full Name	KIT proto-oncogene receptor tyrosine kinase
Synonyms	c-kit
General protein information	Preferred Names KIT proto-oncogene receptor tyrosine kinase Names mast/stem cell growth factor receptor Kit SCFR c-kit receptor mast cell growth factor receptor proto-oncogene c-Kit proto-oncogene tyrosine-protein kinase Kit tyrosine-protein kinase Kit v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001166484.1	NP_001159956.1	mast/stem cell growth factor receptor Kit precursor
XM_005207937.3	XP_005207994.1	mast/stem cell growth factor receptor Kit isoform X1
XM_005207937.4	XP_005207994.2	mast/stem cell growth factor receptor Kit isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	KIT	NP_000213.1
Bos taurus (cattle)	KIT	NP_001159956.1
Rattus norvegicus (Norway rat)	Kit	NP_071600.1
Pan troglodytes (chimpanzee)	KIT	XP_517285.3
Danio rerio (zebrafish)	kita	NP_571128.1
Danio rerio (zebrafish)	kitb	NP_001137390.1
Xenopus tropicalis (tropical clawed frog)	kit	NP_001135545.1
Macaca mulatta (Rhesus monkey)	KIT	XP_001089071.1
Gallus gallus (chicken)	KIT	NP_989692.1
Mus musculus (house mouse)	Kit	NP_001116205.1
Canis lupus familiaris (dog)	KIT	NP_001003181.1

Gene Ontology
Bibliography

Related articles in PubMed

A complex structural variant at the KIT locus in cattle with the Pinzgauer spotting pattern.
Küttel L, Letko A, Häfliger IM, Signer-Hasler H, Joller S, Hirsbrunner G, Mészáros G, Sölkner J, Flury C, Leeb T, Drögemüller C
Animal genetics50(5)423-429(2019 Oct)

Ectopic position of duplicated KIT gene in African Nguni cattle, associated with color sidedness, confirms its shared ancestry with the Bos taurus lineage.
Szczerbal I, Robinson TJ, Duran A, Davey SC, Andersson M, Switonski M
Animal genetics48(1)122-123(2017 Feb)

Genetic heterogeneity at the bovine KIT gene in cattle breeds carrying different putative alleles at the spotting locus.
Fontanesi L, Tazzoli M, Russo V, Beever J
Animal genetics41(3)295-303(2010 Jun)

KIT variants in bovine ovarian cells and corpus luteum.
Koch D, Sakurai M, Hummitzsch K, Hermsdorf T, Erdmann S, Schwalbe S, Stolzenburg JU, Spanel-Borowski K, Ricken AM
Growth factors (Chur, Switzerland)27(2)100-13(2009 Apr)

Fibroblast growth factor 7 stimulates in vitro growth of oocytes originating from bovine early antral follicles.
Cho JH, Itoh T, Sendai Y, Hoshi H
Molecular reproduction and development75(12)1736-43(2008 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following KIT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KIT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa44382	NM_001166484.1 Latest version!	Bos taurus KIT proto-oncogene, receptor tyrosine kinase (KIT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa20398	XM_005207937.3 Latest version!	Bos taurus KIT proto-oncogene receptor tyrosine kinase (KIT), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa235096	XM_005207937.4 Latest version!	Bos taurus KIT proto-oncogene receptor tyrosine kinase (KIT), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

KIT ( NM_001166484.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001159956.1 Bos taurus KIT proto-oncogene, receptor tyrosine kinase (KIT), mRNA.

Price & Availability↑

CloneID	OBa44382
Clone ID Related Accession (Same CDS sequence)	NM_001166484.1
Accession Version	NM_001166484.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2934bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577376000000
Organism	Bos taurus(cattle)
Product	mast/stem cell growth factor receptor Kit precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000006.1. On Oct 25, 2009 this sequence version replaced XM_612028.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571259.37817.1, SRR5721697.1057.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

ATGAGAGGCG CTCGCGGCGC CTGGGATTTC CTCTTCGTTC TGCTGCTCCT GCTCCTCGTC 
CAGACAGGCT CTTCTCAGCC ATCTGTGAGT CCAGGGGAAC TGTCTCTACC ATCTATCCAC 
CCAGCAAAAT CAGAGTTAAT TGTCAGCGTT GGCGACGAGA TTAGGCTGTT ATGCACCGAT 
CCAGGATTTG TCAAGTGGAC TTTTGAGATC CTGGGTCAAC TGAGTGAGAA AACAAACCCG 
GAATGGATCA CCGAGAAAGC AGAGGCCACA AATACAGGCA ATTACACGTG CACCAATAAA 
GGCGGCTTGA GCAGTTCCAT TTATGTGTTT GTTAGAGACC CCGAGAAGCT TTTCCTGATT 
GACCTTCCCT TGTACGGGAA AGAAGAAAAC GACACGCTGG TTCGCTGTCC CCTGACAGAC 
CCCGAGGTGA CCAATTACTC TCTTACGGGG TGTGAGGGGA AACCTCTCCC CAAGGATTTG 
ACATTTGTGG CCGACCCCAA GGCAGGTATC ACAATCAGAA ATGTGAAGCG CGAGTACCAT 
CGGCTCTGTC TGCACTGCTC AGCGAATCAG AGGGGCAAGT CCATGCTGTC GAAGAAATTC 
ACTCTGAAAG TGCGGGCAGC CATCAAAGCT GTGCCAGTTG TGTCTGTGTC CAAAACCAGC 
TATCTTCTCA GGGAAGGAGA GGAATTTGCA GTGACATGCT TGATTAAAGA CGTGTCTAGT 
TCCGTGGACT CTATGTGGAT AAAGGAAAAC AGCCAGCAGA CTAAAGCACA GATGAAGAAG 
AATAGCTGGC ATCAGGGTGA CTTCAGTTAT CTCCGTCAGG AAAGGTTGAC TATCAGCTCA 
GCAAGAGTGA ATGATTCCGG TGTGTTCATG TGTTACGCCA ATAACACTTT TGGATCAGCA 
AATGTCACAA CAACCTTAGA AGTAGTAGAT AAAGGATTCA TTAATATCTT CCCTATGATG 
AACACAACAG TATTTGTAAA TGATGGAGAG AATGTGGATC TGGTTGTTGA ATATGAGGCA 
TATCCCAAAC CTGTACACCG ACAATGGATA TATATGAACA GAACCTCCAC TGATAAGTGG 
GACGATTATC CTAAGTCTGA AAATGAAAGT AATATCAGAT ACGTAAATGA ACTTCATCTA 
ACCAGATTAA AAGGGACTGA AGGAGGCACT TACACATTTC ACGTGTCCAA TTCTGATGTC 
AATTCTTCCG TGACATTTAA TGTTTACGTG AACACAAAAC CAGAAATCCT GACGCATGAC 
AGGCTGGTGA ATGGCATGCT ACAGTGCGTG GCCGCAGGGT TCCCGGAGCC AACCATCGAT 
TGGTACTTTT GTCCAGGAAC TGAGCAGAGG TGTTCTGTTC CCGTTGGGCC AGTGGATGTA 
CAGATCCAAA ACTCATCTGT CTCACCATTT GGAAAACTAG TGGTTTATAG CACCATTGAT 
GACAGCACAT TCAAACACAA TGGGACGGTG GAGTGCAGGG CTTATAACGA TGTGGGCAAG 
AGTTCTGCCT CTTTTAACTT TGCATTTAAA GGTAACAGCA AAGAACAAAT CCATGCTCAC 
ACCCTGTTCA CGCCGTTGCT GATTGGTTTT GTGATCGCAG CTGGTTTAAT GTGTATCTTC 
GTGATGATTC TTACATACAA ATATTTGCAG AAACCCATGT ATGAAGTACA GTGGAAAGTT 
GTCGAGGAGA TAAATGGAAA CAATTATGTT TACATAGACC CAACACAACT TCCTTATGAT 
CACAAATGGG AGTTTCCCAG GAACAGGCTG AGTTTTGGGA AAACCCTGGG TGCTGGCGCC 
TTCGGGAAAG TTGTTGAGGC CACCGCTTAT GGCTTAATTA AATCAGATGC AGCCATGACT 
GTTGCTGTCA AGATGCTCAA ACCAAGCGCC CATTTAACCG AACGAGAAGC CCTAATGTCT 
GAACTCAAAG TCTTGAGTTA CCTCGGTAAT CATATGAATA TTGTGAATCT TCTGGGAGCG 
TGCACCATTG GAGGGCCCAC CCTGGTCATT ACAGAATATT GTTGCTATGG TGATCTTCTG 
AATTTTTTGA GAAGAAAACG TGATTCATTT ATTTGCTCAA AGCAGGAAGA TCACGCCGAA 
GTGGCGCTTT ATAAGAACCT TCTTCATTCA AAGGAGTCTT CCTGCAATGA TAGTACTAAT 
GAGTACATGG ACATGAAACC TGGAGTTTCT TATGTTGTAC CAACCAAGGC AGACAAGAGG 
AGATCTGCAA GAATAGGTTC ATACATAGAA AGAGACGTGA CTCCTGCTAT CATGGAAGAT 
GATGAGCTGG CCCTGGACCT GGAGGACTTG CTGAGCTTTT CTTACCAGGT GGCAAAAGGC 
ATGGCGTTCC TTGCCTCAAA GAATTGTATT CATAGAGACT TGGCAGCCAG AAATATCCTC 
CTTACTCATG GTCGAATCAC AAAGATTTGT GATTTTGGTC TAGCCAGAGA CATCAAGAAT 
GATTCTAATT ATGTGGTCAA AGGAAACGCT CGACTCCCTG TGAAGTGGAT GGCACCAGAG 
AGTATTTTCA ACTGTGTATA CACATTTGAA AGTGATGTCT GGTCCTATGG GATTTTTCTG 
TGGGAGCTGT TCTCTTTAGG AAGCAGCCCC TACCCTGGAA TGCCAGTCGA TTCTAAGTTC 
TACAAGATGA TCAAGGAAGG TTTCCGAATG CTCAGCCCCG AGCATGCACC TGCGGAAATG 
TATGACATCA TGAAGACCTG CTGGGATGCT GATCCCTTGA AAAGGCCAAC ATTTAAGCAG 
ATTGTGCAGC TGATTGAGAA GCAGATCTCA GAGAGCACCA ATCATATTTA TTCCAACTTA 
GCAAACTGCA GCCCCCACCG GGAGAACCCC GCCGTGGACC ATTCTGTGCG CATCAACTCT 
GTGGGCAGCA GCGCCTCCTC CACGCAGCCT CTGCTTGTCC ACGAAGATGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001159956.1
CDS	103..3036
Translation	MRGARGAWDFLFVLLLLLLVQTGSSQPSVSPGELSLPSIHPAKSELIVSVGDEIRLLCTDPGFVKWTFEILGQLSEKTNPEWITEKAEATNTGNYTCTNKGGLSSSIYVFVRDPEKLFLIDLPLYGKEENDTLVRCPLTDPEVTNYSLTGCEGKPLPKDLTFVADPKAGITIRNVKREYHRLCLHCSANQRGKSMLSKKFTLKVRAAIKAVPVVSVSKTSYLLREGEEFAVTCLIKDVSSSVDSMWIKENSQQTKAQMKKNSWHQGDFSYLRQERLTISSARVNDSGVFMCYANNTFGSANVTTTLEVVDKGFINIFPMMNTTVFVNDGENVDLVVEYEAYPKPVHRQWIYMNRTSTDKWDDYPKSENESNIRYVNELHLTRLKGTEGGTYTFHVSNSDVNSSVTFNVYVNTKPEILTHDRLVNGMLQCVAAGFPEPTIDWYFCPGTEQRCSVPVGPVDVQIQNSSVSPFGKLVVYSTIDDSTFKHNGTVECRAYNDVGKSSASFNFAFKGNSKEQIHAHTLFTPLLIGFVIAAGLMCIFVMILTYKYLQKPMYEVQWKVVEEINGNNYVYIDPTQLPYDHKWEFPRNRLSFGKTLGAGAFGKVVEATAYGLIKSDAAMTVAVKMLKPSAHLTEREALMSELKVLSYLGNHMNIVNLLGACTIGGPTLVITEYCCYGDLLNFLRRKRDSFICSKQEDHAEVALYKNLLHSKESSCNDSTNEYMDMKPGVSYVVPTKADKRRSARIGSYIERDVTPAIMEDDELALDLEDLLSFSYQVAKGMAFLASKNCIHRDLAARNILLTHGRITKICDFGLARDIKNDSNYVVKGNARLPVKWMAPESIFNCVYTFESDVWSYGIFLWELFSLGSSPYPGMPVDSKFYKMIKEGFRMLSPEHAPAEMYDIMKTCWDADPLKRPTFKQIVQLIEKQISESTNHIYSNLANCSPHRENPAVDHSVRINSVGSSASSTQPLLVHEDV

Target ORF information:

RefSeq Version	NM_001166484.1
Organism	Bos taurus(cattle)
Definition	Bos taurus KIT proto-oncogene, receptor tyrosine kinase (KIT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001166484.1

ORF Insert Sequence:

ATGAGAGGCG CTCGCGGCGC CTGGGATTTC CTCTTCGTTC TGCTGCTCCT GCTCCTCGTC 
CAGACAGGCT CTTCTCAGCC ATCTGTGAGT CCAGGGGAAC TGTCTCTACC ATCTATCCAC 
CCAGCAAAAT CAGAGTTAAT TGTCAGCGTT GGCGACGAGA TTAGGCTGTT ATGCACCGAT 
CCAGGATTTG TCAAGTGGAC TTTTGAGATC CTGGGTCAAC TGAGTGAGAA AACAAACCCG 
GAATGGATCA CCGAGAAAGC AGAGGCCACA AATACAGGCA ATTACACGTG CACCAATAAA 
GGCGGCTTGA GCAGTTCCAT TTATGTGTTT GTTAGAGACC CCGAGAAGCT TTTCCTGATT 
GACCTTCCCT TGTACGGGAA AGAAGAAAAC GACACGCTGG TTCGCTGTCC CCTGACAGAC 
CCCGAGGTGA CCAATTACTC TCTTACGGGG TGTGAGGGGA AACCTCTCCC CAAGGATTTG 
ACATTTGTGG CCGACCCCAA GGCAGGTATC ACAATCAGAA ATGTGAAGCG CGAGTACCAT 
CGGCTCTGTC TGCACTGCTC AGCGAATCAG AGGGGCAAGT CCATGCTGTC GAAGAAATTC 
ACTCTGAAAG TGCGGGCAGC CATCAAAGCT GTGCCAGTTG TGTCTGTGTC CAAAACCAGC 
TATCTTCTCA GGGAAGGAGA GGAATTTGCA GTGACATGCT TGATTAAAGA CGTGTCTAGT 
TCCGTGGACT CTATGTGGAT AAAGGAAAAC AGCCAGCAGA CTAAAGCACA GATGAAGAAG 
AATAGCTGGC ATCAGGGTGA CTTCAGTTAT CTCCGTCAGG AAAGGTTGAC TATCAGCTCA 
GCAAGAGTGA ATGATTCCGG TGTGTTCATG TGTTACGCCA ATAACACTTT TGGATCAGCA 
AATGTCACAA CAACCTTAGA AGTAGTAGAT AAAGGATTCA TTAATATCTT CCCTATGATG 
AACACAACAG TATTTGTAAA TGATGGAGAG AATGTGGATC TGGTTGTTGA ATATGAGGCA 
TATCCCAAAC CTGTACACCG ACAATGGATA TATATGAACA GAACCTCCAC TGATAAGTGG 
GACGATTATC CTAAGTCTGA AAATGAAAGT AATATCAGAT ACGTAAATGA ACTTCATCTA 
ACCAGATTAA AAGGGACTGA AGGAGGCACT TACACATTTC ACGTGTCCAA TTCTGATGTC 
AATTCTTCCG TGACATTTAA TGTTTACGTG AACACAAAAC CAGAAATCCT GACGCATGAC 
AGGCTGGTGA ATGGCATGCT ACAGTGCGTG GCCGCAGGGT TCCCGGAGCC AACCATCGAT 
TGGTACTTTT GTCCAGGAAC TGAGCAGAGG TGTTCTGTTC CCGTTGGGCC AGTGGATGTA 
CAGATCCAAA ACTCATCTGT CTCACCATTT GGAAAACTAG TGGTTTATAG CACCATTGAT 
GACAGCACAT TCAAACACAA TGGGACGGTG GAGTGCAGGG CTTATAACGA TGTGGGCAAG 
AGTTCTGCCT CTTTTAACTT TGCATTTAAA GGTAACAGCA AAGAACAAAT CCATGCTCAC 
ACCCTGTTCA CGCCGTTGCT GATTGGTTTT GTGATCGCAG CTGGTTTAAT GTGTATCTTC 
GTGATGATTC TTACATACAA ATATTTGCAG AAACCCATGT ATGAAGTACA GTGGAAAGTT 
GTCGAGGAGA TAAATGGAAA CAATTATGTT TACATAGACC CAACACAACT TCCTTATGAT 
CACAAATGGG AGTTTCCCAG GAACAGGCTG AGTTTTGGGA AAACCCTGGG TGCTGGCGCC 
TTCGGGAAAG TTGTTGAGGC CACCGCTTAT GGCTTAATTA AATCAGATGC AGCCATGACT 
GTTGCTGTCA AGATGCTCAA ACCAAGCGCC CATTTAACCG AACGAGAAGC CCTAATGTCT 
GAACTCAAAG TCTTGAGTTA CCTCGGTAAT CATATGAATA TTGTGAATCT TCTGGGAGCG 
TGCACCATTG GAGGGCCCAC CCTGGTCATT ACAGAATATT GTTGCTATGG TGATCTTCTG 
AATTTTTTGA GAAGAAAACG TGATTCATTT ATTTGCTCAA AGCAGGAAGA TCACGCCGAA 
GTGGCGCTTT ATAAGAACCT TCTTCATTCA AAGGAGTCTT CCTGCAATGA TAGTACTAAT 
GAGTACATGG ACATGAAACC TGGAGTTTCT TATGTTGTAC CAACCAAGGC AGACAAGAGG 
AGATCTGCAA GAATAGGTTC ATACATAGAA AGAGACGTGA CTCCTGCTAT CATGGAAGAT 
GATGAGCTGG CCCTGGACCT GGAGGACTTG CTGAGCTTTT CTTACCAGGT GGCAAAAGGC 
ATGGCGTTCC TTGCCTCAAA GAATTGTATT CATAGAGACT TGGCAGCCAG AAATATCCTC 
CTTACTCATG GTCGAATCAC AAAGATTTGT GATTTTGGTC TAGCCAGAGA CATCAAGAAT 
GATTCTAATT ATGTGGTCAA AGGAAACGCT CGACTCCCTG TGAAGTGGAT GGCACCAGAG 
AGTATTTTCA ACTGTGTATA CACATTTGAA AGTGATGTCT GGTCCTATGG GATTTTTCTG 
TGGGAGCTGT TCTCTTTAGG AAGCAGCCCC TACCCTGGAA TGCCAGTCGA TTCTAAGTTC 
TACAAGATGA TCAAGGAAGG TTTCCGAATG CTCAGCCCCG AGCATGCACC TGCGGAAATG 
TATGACATCA TGAAGACCTG CTGGGATGCT GATCCCTTGA AAAGGCCAAC ATTTAAGCAG 
ATTGTGCAGC TGATTGAGAA GCAGATCTCA GAGAGCACCA ATCATATTTA TTCCAACTTA 
GCAAACTGCA GCCCCCACCG GGAGAACCCC GCCGTGGACC ATTCTGTGCG CATCAACTCT 
GTGGGCAGCA GCGCCTCCTC CACGCAGCCT CTGCTTGTCC ACGAAGATGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIT ( XM_005207937.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005207994.1 Bos taurus KIT proto-oncogene receptor tyrosine kinase (KIT), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa20398
Clone ID Related Accession (Same CDS sequence)	XM_005207937.3
Accession Version	XM_005207937.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2922bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	mast/stem cell growth factor receptor Kit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103975.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005207937.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGGCG CTCGCGGCGC CTGGGATTTC CTCTTCGTTC TGCTGCTCCT GCTCCTCGTC 
CAGACAGGCT CTTCTCAGCC ATCTGTGAGT CCAGGGGAAC TGTCTCTACC ATCTATCCAC 
CCAGCAAAAT CAGAGTTAAT TGTCAGCGTT GGCGACGAGA TTAGGCTGTT ATGCACCGAT 
CCAGGATTTG TCAAGTGGAC TTTTGAGATC CTGGGTCAAC TGAGTGAGAA AACAAACCCG 
GAATGGATCA CCGAGAAAGC AGAGGCCACA AATACAGGCA ATTACACGTG CACCAATAAA 
GGCGGCTTGA GCAGTTCCAT TTATGTGTTT GTTAGAGACC CCGAGAAGCT TTTCCTGATT 
GACCTTCCCT TGTACGGGAA AGAAGAAAAC GACACGCTGG TTCGCTGTCC CCTGACAGAC 
CCCGAGGTGA CCAATTACTC TCTTACGGGG TGTGAGGGGA AACCTCTCCC CAAGGATTTG 
ACATTTGTGG CCGACCCCAA GGCAGGTATC ACAATCAGAA ATGTGAAGCG CGAGTACCAT 
CGGCTCTGTC TGCACTGCTC AGCGAATCAG AGGGGCAAGT CCATGCTGTC GAAGAAATTC 
ACTCTGAAAG TGCGGGCAGC CATCAAAGCT GTGCCAGTTG TGTCTGTGTC CAAAACCAGC 
TATCTTCTCA GGGAAGGAGA GGAATTTGCA GTGACATGCT TGATTAAAGA CGTGTCTAGT 
TCCGTGGACT CTATGTGGAT AAAGGAAAAC AGCCAGCAGA CTAAAGCACA GACGAAGAAG 
AATAGCTGGC ATCAGGGTGA CTTCAGTTAT CTCCGTCAGG AAAGGTTGAC TATCAGCTCA 
GCAAGAGTGA ATGATTCCGG TGTGTTCATG TGTTACGCCA ATAACACTTT TGGATCAGCA 
AATGTCACAA CAACCTTAGA AGTAGTAGAT AAAGGATTCA TTAATATCTT CCCTATGATG 
AACACAACAG TATTTGTAAA TGATGGAGAG AATGTGGATC TGGTTGTTGA ATATGAGGCA 
TATCCCAAAC CTGTACACCG ACAATGGATA TATATGAACA GAACCTCCAC TGATAAGTGG 
GACGATTATC CTAAGTCTGA AAATGAAAGT AATATCAGAT ACGTAAATGA ACTTCATCTA 
ACCAGATTAA AAGGGACTGA AGGAGGCACT TACACATTTC ACGTGTCCAA TTCTGATGTC 
AATTCTTCCG TGACATTTAA TGTTTACGTG AACACAAAAC CAGAAATCCT GACGCATGAC 
AGGCTGGTGA ATGGCATGCT ACAGTGCGTG GCCGCAGGGT TCCCGGAGCC AACCATCGAT 
TGGTACTTTT GTCCAGGAAC TGAGCAGAGG TGTTCTGTTC CCGTTGGGCC AGTGGATGTA 
CAGATCCAAA ACTCATCTGT CTCACCATTT GGAAAACTAG TGGTTTATAG CACCATTGAT 
GACAGCACAT TCAAACACAA TGGGACGGTG GAGTGCAGGG CTTATAACGA TGTGGGCAAG 
AGTTCTGCCT CTTTTAACTT TGCATTTAAA GAACAAATCC ATGCTCACAC CCTGTTCACG 
CCGTTGCTGA TTGGTTTTGT GATCGCAGCT GGTTTAATGT GTATCTTCGT GATGATTCTT 
ACATACAAAT ATTTGCAGAA ACCCATGTAT GAAGTACAGT GGAAAGTTGT CGAGGAGATA 
AATGGAAACA ATTATGTTTA CATAGACCCA ACACAACTTC CTTATGATCA CAAATGGGAG 
TTTCCCAGGA ACAGGCTGAG TTTTGGGAAA ACCCTGGGTG CTGGCGCCTT CGGGAAAGTT 
GTTGAGGCCA CCGCTTATGG CTTAATTAAA TCAGATGCAG CCATGACTGT TGCTGTCAAG 
ATGCTCAAAC CAAGCGCCCA TTTAACCGAA CGAGAAGCCC TAATGTCTGA ACTCAAAGTC 
TTGAGTTACC TCGGTAATCA TATGAATATT GTGAATCTTC TGGGAGCGTG CACCATTGGA 
GGGCCCACCC TGGTCATTAC AGAATATTGT TGCTATGGTG ATCTTCTGAA TTTTTTGAGA 
AGAAAACGTG ATTCATTTAT TTGCTCAAAG CAGGAAGATC ACGCCGAAGT GGCGCTTTAT 
AAGAACCTTC TTCATTCAAA GGAGTCTTCC TGCAATGATA GTACTAATGA GTACATGGAC 
ATGAAACCTG GAGTTTCTTA TGTTGTACCA ACCAAGGCAG ACAAGAGGAG ATCTGCAAGA 
ATAGGTTCAT ACATAGAAAG AGACGTGACT CCTGCTATCA TGGAAGATGA TGAGCTGGCC 
CTGGACCTGG AGGACTTGCT GAGCTTTTCT TACCAGGTGG CAAAAGGCAT GGCGTTCCTT 
GCCTCAAAGA ATTGTATTCA TAGAGACTTG GCAGCCAGAA ATATCCTCCT TACTCATGGT 
CGAATCACAA AGATTTGTGA TTTTGGTCTA GCCAGAGACA TCAAGAATGA TTCTAATTAT 
GTGGTCAAAG GAAACGCTCG ACTCCCTGTG AAGTGGATGG CACCAGAGAG TATTTTCAAC 
TGTGTATACA CATTTGAAAG TGATGTCTGG TCCTATGGGA TTTTTCTGTG GGAGCTGTTC 
TCTTTAGGAA GCAGCCCCTA CCCTGGAATG CCAGTCGATT CTAAGTTCTA CAAGATGATC 
AAGGAAGGTT TCCGAATGCT CAGCCCCGAG CATGCACCTG CGGAAATGTA TGACATCATG 
AAGACCTGCT GGGATGCTGA TCCCTTGAAA AGGCCAACAT TTAAGCAGAT TGTGCAGCTG 
ATTGAGAAGC AGATCTCAGA GAGCACCAAT CATATTTATT CCAACTTAGC AAACTGCAGC 
CCCCACCGGG AGAACCCCGC CGTGGACCAT TCTGTGCGCA TCAACTCTGT GGGCAGCAGC 
GCCTCCTCCA CGCAGCCTCT GCTTGTCCAC GAAGATGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005207994.1
CDS	82..3003
Translation	MRGARGAWDFLFVLLLLLLVQTGSSQPSVSPGELSLPSIHPAKSELIVSVGDEIRLLCTDPGFVKWTFEILGQLSEKTNPEWITEKAEATNTGNYTCTNKGGLSSSIYVFVRDPEKLFLIDLPLYGKEENDTLVRCPLTDPEVTNYSLTGCEGKPLPKDLTFVADPKAGITIRNVKREYHRLCLHCSANQRGKSMLSKKFTLKVRAAIKAVPVVSVSKTSYLLREGEEFAVTCLIKDVSSSVDSMWIKENSQQTKAQTKKNSWHQGDFSYLRQERLTISSARVNDSGVFMCYANNTFGSANVTTTLEVVDKGFINIFPMMNTTVFVNDGENVDLVVEYEAYPKPVHRQWIYMNRTSTDKWDDYPKSENESNIRYVNELHLTRLKGTEGGTYTFHVSNSDVNSSVTFNVYVNTKPEILTHDRLVNGMLQCVAAGFPEPTIDWYFCPGTEQRCSVPVGPVDVQIQNSSVSPFGKLVVYSTIDDSTFKHNGTVECRAYNDVGKSSASFNFAFKEQIHAHTLFTPLLIGFVIAAGLMCIFVMILTYKYLQKPMYEVQWKVVEEINGNNYVYIDPTQLPYDHKWEFPRNRLSFGKTLGAGAFGKVVEATAYGLIKSDAAMTVAVKMLKPSAHLTEREALMSELKVLSYLGNHMNIVNLLGACTIGGPTLVITEYCCYGDLLNFLRRKRDSFICSKQEDHAEVALYKNLLHSKESSCNDSTNEYMDMKPGVSYVVPTKADKRRSARIGSYIERDVTPAIMEDDELALDLEDLLSFSYQVAKGMAFLASKNCIHRDLAARNILLTHGRITKICDFGLARDIKNDSNYVVKGNARLPVKWMAPESIFNCVYTFESDVWSYGIFLWELFSLGSSPYPGMPVDSKFYKMIKEGFRMLSPEHAPAEMYDIMKTCWDADPLKRPTFKQIVQLIEKQISESTNHIYSNLANCSPHRENPAVDHSVRINSVGSSASSTQPLLVHEDV

Target ORF information:

RefSeq Version	XM_005207937.3
Organism	Bos taurus(cattle)
Definition	Bos taurus KIT proto-oncogene receptor tyrosine kinase (KIT), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005207937.3

ORF Insert Sequence:

ATGAGAGGCG CTCGCGGCGC CTGGGATTTC CTCTTCGTTC TGCTGCTCCT GCTCCTCGTC 
CAGACAGGCT CTTCTCAGCC ATCTGTGAGT CCAGGGGAAC TGTCTCTACC ATCTATCCAC 
CCAGCAAAAT CAGAGTTAAT TGTCAGCGTT GGCGACGAGA TTAGGCTGTT ATGCACCGAT 
CCAGGATTTG TCAAGTGGAC TTTTGAGATC CTGGGTCAAC TGAGTGAGAA AACAAACCCG 
GAATGGATCA CCGAGAAAGC AGAGGCCACA AATACAGGCA ATTACACGTG CACCAATAAA 
GGCGGCTTGA GCAGTTCCAT TTATGTGTTT GTTAGAGACC CCGAGAAGCT TTTCCTGATT 
GACCTTCCCT TGTACGGGAA AGAAGAAAAC GACACGCTGG TTCGCTGTCC CCTGACAGAC 
CCCGAGGTGA CCAATTACTC TCTTACGGGG TGTGAGGGGA AACCTCTCCC CAAGGATTTG 
ACATTTGTGG CCGACCCCAA GGCAGGTATC ACAATCAGAA ATGTGAAGCG CGAGTACCAT 
CGGCTCTGTC TGCACTGCTC AGCGAATCAG AGGGGCAAGT CCATGCTGTC GAAGAAATTC 
ACTCTGAAAG TGCGGGCAGC CATCAAAGCT GTGCCAGTTG TGTCTGTGTC CAAAACCAGC 
TATCTTCTCA GGGAAGGAGA GGAATTTGCA GTGACATGCT TGATTAAAGA CGTGTCTAGT 
TCCGTGGACT CTATGTGGAT AAAGGAAAAC AGCCAGCAGA CTAAAGCACA GACGAAGAAG 
AATAGCTGGC ATCAGGGTGA CTTCAGTTAT CTCCGTCAGG AAAGGTTGAC TATCAGCTCA 
GCAAGAGTGA ATGATTCCGG TGTGTTCATG TGTTACGCCA ATAACACTTT TGGATCAGCA 
AATGTCACAA CAACCTTAGA AGTAGTAGAT AAAGGATTCA TTAATATCTT CCCTATGATG 
AACACAACAG TATTTGTAAA TGATGGAGAG AATGTGGATC TGGTTGTTGA ATATGAGGCA 
TATCCCAAAC CTGTACACCG ACAATGGATA TATATGAACA GAACCTCCAC TGATAAGTGG 
GACGATTATC CTAAGTCTGA AAATGAAAGT AATATCAGAT ACGTAAATGA ACTTCATCTA 
ACCAGATTAA AAGGGACTGA AGGAGGCACT TACACATTTC ACGTGTCCAA TTCTGATGTC 
AATTCTTCCG TGACATTTAA TGTTTACGTG AACACAAAAC CAGAAATCCT GACGCATGAC 
AGGCTGGTGA ATGGCATGCT ACAGTGCGTG GCCGCAGGGT TCCCGGAGCC AACCATCGAT 
TGGTACTTTT GTCCAGGAAC TGAGCAGAGG TGTTCTGTTC CCGTTGGGCC AGTGGATGTA 
CAGATCCAAA ACTCATCTGT CTCACCATTT GGAAAACTAG TGGTTTATAG CACCATTGAT 
GACAGCACAT TCAAACACAA TGGGACGGTG GAGTGCAGGG CTTATAACGA TGTGGGCAAG 
AGTTCTGCCT CTTTTAACTT TGCATTTAAA GAACAAATCC ATGCTCACAC CCTGTTCACG 
CCGTTGCTGA TTGGTTTTGT GATCGCAGCT GGTTTAATGT GTATCTTCGT GATGATTCTT 
ACATACAAAT ATTTGCAGAA ACCCATGTAT GAAGTACAGT GGAAAGTTGT CGAGGAGATA 
AATGGAAACA ATTATGTTTA CATAGACCCA ACACAACTTC CTTATGATCA CAAATGGGAG 
TTTCCCAGGA ACAGGCTGAG TTTTGGGAAA ACCCTGGGTG CTGGCGCCTT CGGGAAAGTT 
GTTGAGGCCA CCGCTTATGG CTTAATTAAA TCAGATGCAG CCATGACTGT TGCTGTCAAG 
ATGCTCAAAC CAAGCGCCCA TTTAACCGAA CGAGAAGCCC TAATGTCTGA ACTCAAAGTC 
TTGAGTTACC TCGGTAATCA TATGAATATT GTGAATCTTC TGGGAGCGTG CACCATTGGA 
GGGCCCACCC TGGTCATTAC AGAATATTGT TGCTATGGTG ATCTTCTGAA TTTTTTGAGA 
AGAAAACGTG ATTCATTTAT TTGCTCAAAG CAGGAAGATC ACGCCGAAGT GGCGCTTTAT 
AAGAACCTTC TTCATTCAAA GGAGTCTTCC TGCAATGATA GTACTAATGA GTACATGGAC 
ATGAAACCTG GAGTTTCTTA TGTTGTACCA ACCAAGGCAG ACAAGAGGAG ATCTGCAAGA 
ATAGGTTCAT ACATAGAAAG AGACGTGACT CCTGCTATCA TGGAAGATGA TGAGCTGGCC 
CTGGACCTGG AGGACTTGCT GAGCTTTTCT TACCAGGTGG CAAAAGGCAT GGCGTTCCTT 
GCCTCAAAGA ATTGTATTCA TAGAGACTTG GCAGCCAGAA ATATCCTCCT TACTCATGGT 
CGAATCACAA AGATTTGTGA TTTTGGTCTA GCCAGAGACA TCAAGAATGA TTCTAATTAT 
GTGGTCAAAG GAAACGCTCG ACTCCCTGTG AAGTGGATGG CACCAGAGAG TATTTTCAAC 
TGTGTATACA CATTTGAAAG TGATGTCTGG TCCTATGGGA TTTTTCTGTG GGAGCTGTTC 
TCTTTAGGAA GCAGCCCCTA CCCTGGAATG CCAGTCGATT CTAAGTTCTA CAAGATGATC 
AAGGAAGGTT TCCGAATGCT CAGCCCCGAG CATGCACCTG CGGAAATGTA TGACATCATG 
AAGACCTGCT GGGATGCTGA TCCCTTGAAA AGGCCAACAT TTAAGCAGAT TGTGCAGCTG 
ATTGAGAAGC AGATCTCAGA GAGCACCAAT CATATTTATT CCAACTTAGC AAACTGCAGC 
CCCCACCGGG AGAACCCCGC CGTGGACCAT TCTGTGCGCA TCAACTCTGT GGGCAGCAGC 
GCCTCCTCCA CGCAGCCTCT GCTTGTCCAC GAAGATGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIT ( XM_005207937.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005207994.2 Bos taurus KIT proto-oncogene receptor tyrosine kinase (KIT), transcript variant X1, mRNA.

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CloneID	OBa235096
Clone ID Related Accession (Same CDS sequence)	XM_005207937.4
Accession Version	XM_005207937.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2922bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	mast/stem cell growth factor receptor Kit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005207937.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGGCG CTCGCGGCGC CTGGGATTTC CTCTTCGTTC TGCTGCTCCT GCTCCTCGTC 
CAGACAGGCT CTTCTCAGCC ATCTGTGAGT CCAGGGGAAC TGTCTCTACC ATCTATCCAC 
CCAGCAAAAT CAGAGTTAAT TGTCAGCGTT GGCGACGAGA TTAGGCTGTT ATGCACCGAT 
CCAGGATTTG TCAAGTGGAC TTTTGAGATC CTGGGTCAAC TGAGTGAGAA AACAAACCCG 
GAATGGATCA CCGAGAAAGC AGAGGCCACA AATACAGGCA ATTACACGTG CACCAATAAA 
GGCGGCTTGA GCAGTTCCAT TTATGTGTTT GTTAGAGACC CCGAGAAGCT TTTCCTGATT 
GACCTTCCCT TGTACGGGAA AGAAGAAAAC GACACGCTGG TTCGCTGTCC CCTGACAGAC 
CCCGAGGTGA CCAATTACTC TCTTACGGGG TGTGAGGGGA AACCTCTCCC CAAGGATTTG 
ACATTTGTGG CCGACCCCAA GGCAGGTATC ACAATCAGAA ATGTGAAGCG CGAGTACCAT 
CGGCTCTGTC TGCACTGCTC AGCGAATCAG AGGGGCAAGT CCATGCTGTC GAAGAAATTC 
ACTCTGAAAG TGCGGGCAGC CATCAAAGCT GTGCCAGTTG TGTCTGTGTC CAAAACCAGC 
TATCTTCTCA GGGAAGGAGA GGAATTTGCA GTGACATGCT TGATTAAAGA CGTGTCTAGT 
TCCGTGGACT CTATGTGGAT AAAGGAAAAC AGCCAGCAGA CTAAAGCACA GATGAAGAAG 
AATAGCTGGC ATCAGGGTGA CTTCAGTTAT CTCCGTCAGG AAAGGTTGAC TATCAGCTCA 
GCAAGAGTGA ATGATTCCGG TGTGTTCATG TGTTACGCCA ATAACACTTT TGGATCAGCA 
AATGTCACAA CAACCTTAGA AGTAGTAGAT AAAGGATTCA TTAATATCTT CCCTATGATG 
AACACAACAG TATTTGTAAA TGATGGAGAG AATGTGGATC TGGTTGTTGA ATATGAGGCA 
TATCCCAAAC CTGTACACCG ACAATGGATA TATATGAACA GAACCTCCAC TGATAAGTGG 
GACGATTATC CTAAGTCTGA AAATGAAAGT AATATCAGAT ACGTAAATGA ACTTCATCTA 
ACCAGATTAA AAGGGACTGA AGGAGGCACT TACACATTTC ACGTGTCCAA TTCTGATGTC 
AATTCTTCCG TGACATTTAA TGTTTACGTG AACACAAAAC CAGAAATCCT GACGCATGAC 
AGGCTGGTGA ATGGCATGCT ACAGTGCGTG GCCGCAGGGT TCCCGGAGCC AACCATCGAT 
TGGTACTTTT GTCCAGGAAC TGAGCAGAGG TGTTCTGTTC CCGTTGGGCC AGTGGATGTA 
CAGATCCAAA ACTCATCTGT CTCACCATTT GGAAAACTAG TGGTTTATAG CACCATTGAT 
GACAGCACAT TCAAACACAA TGGGACGGTG GAGTGCAGGG CTTATAACGA TGTGGGCAAG 
AGTTCTGCCT CTTTTAACTT TGCATTTAAA GAACAAATCC ATGCTCACAC CCTGTTCACG 
CCGTTGCTGA TTGGTTTTGT GATCGCAGCT GGTTTAATGT GTATCTTCGT GATGATTCTT 
ACATACAAAT ATTTGCAGAA ACCCATGTAT GAAGTACAGT GGAAAGTTGT CGAGGAGATA 
AATGGAAACA ATTATGTTTA CATAGACCCA ACACAACTTC CTTATGATCA CAAATGGGAG 
TTTCCCAGGA ACAGGCTGAG TTTTGGGAAA ACCCTGGGTG CTGGCGCCTT CGGGAAAGTT 
GTTGAGGCCA CCGCTTATGG CTTAATTAAA TCAGATGCAG CCATGACTGT TGCTGTCAAG 
ATGCTCAAAC CAAGCGCCCA TTTAACCGAA CGAGAAGCCC TAATGTCTGA ACTCAAAGTC 
TTGAGTTACC TCGGTAATCA TATGAATATT GTGAATCTTC TGGGAGCGTG CACCATTGGA 
GGGCCCACCC TGGTCATTAC AGAATATTGT TGCTATGGTG ATCTTCTGAA TTTTTTGAGA 
AGAAAACGTG ATTCATTTAT TTGCTCAAAG CAGGAAGATC ACGCCGAAGT GGCGCTTTAT 
AAGAACCTTC TTCATTCAAA GGAGTCTTCC TGCAATGATA GTACTAATGA GTACATGGAC 
ATGAAACCTG GAGTTTCTTA TGTTGTACCA ACCAAGGCAG ACAAGAGGAG ATCTGCAAGA 
ATAGGTTCAT ACATAGAAAG AGACGTGACT CCTGCTATCA TGGAAGATGA TGAGCTGGCC 
CTGGACCTGG AGGACTTGCT GAGCTTTTCT TACCAGGTGG CAAAAGGCAT GGCGTTCCTT 
GCCTCAAAGA ATTGTATTCA TAGAGACTTG GCAGCCAGAA ATATCCTCCT TACTCATGGT 
CGAATCACAA AGATTTGTGA TTTTGGTCTA GCCAGAGACA TCAAGAATGA TTCTAATTAT 
GTGGTCAAAG GAAACGCTCG ACTCCCTGTG AAGTGGATGG CACCAGAGAG TATTTTCAAC 
TGTGTATACA CATTTGAAAG TGATGTCTGG TCCTATGGGA TTTTTCTGTG GGAGCTGTTC 
TCTTTAGGAA GCAGCCCCTA CCCTGGAATG CCAGTCGATT CTAAGTTCTA CAAGATGATC 
AAGGAAGGTT TCCGAATGCT CAGCCCCGAG CATGCACCTG CGGAAATGTA TGACATCATG 
AAGACCTGCT GGGATGCTGA TCCCTTGAAA AGGCCAACAT TTAAGCAGAT TGTGCAGCTG 
ATTGAGAAGC AGATCTCAGA GAGCACCAAT CATATTTATT CCAACTTAGC AAACTGCAGC 
CCCCACCGGG AGAACCCCGC CGTGGACCAT TCTGTGCGCA TCAACTCTGT GGGCAGCAGC 
GCCTCCTCCA CGCAGCCTCT GCTTGTCCAC GAAGATGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005207994.2
CDS	114..3035
Translation	MRGARGAWDFLFVLLLLLLVQTGSSQPSVSPGELSLPSIHPAKSELIVSVGDEIRLLCTDPGFVKWTFEILGQLSEKTNPEWITEKAEATNTGNYTCTNKGGLSSSIYVFVRDPEKLFLIDLPLYGKEENDTLVRCPLTDPEVTNYSLTGCEGKPLPKDLTFVADPKAGITIRNVKREYHRLCLHCSANQRGKSMLSKKFTLKVRAAIKAVPVVSVSKTSYLLREGEEFAVTCLIKDVSSSVDSMWIKENSQQTKAQMKKNSWHQGDFSYLRQERLTISSARVNDSGVFMCYANNTFGSANVTTTLEVVDKGFINIFPMMNTTVFVNDGENVDLVVEYEAYPKPVHRQWIYMNRTSTDKWDDYPKSENESNIRYVNELHLTRLKGTEGGTYTFHVSNSDVNSSVTFNVYVNTKPEILTHDRLVNGMLQCVAAGFPEPTIDWYFCPGTEQRCSVPVGPVDVQIQNSSVSPFGKLVVYSTIDDSTFKHNGTVECRAYNDVGKSSASFNFAFKEQIHAHTLFTPLLIGFVIAAGLMCIFVMILTYKYLQKPMYEVQWKVVEEINGNNYVYIDPTQLPYDHKWEFPRNRLSFGKTLGAGAFGKVVEATAYGLIKSDAAMTVAVKMLKPSAHLTEREALMSELKVLSYLGNHMNIVNLLGACTIGGPTLVITEYCCYGDLLNFLRRKRDSFICSKQEDHAEVALYKNLLHSKESSCNDSTNEYMDMKPGVSYVVPTKADKRRSARIGSYIERDVTPAIMEDDELALDLEDLLSFSYQVAKGMAFLASKNCIHRDLAARNILLTHGRITKICDFGLARDIKNDSNYVVKGNARLPVKWMAPESIFNCVYTFESDVWSYGIFLWELFSLGSSPYPGMPVDSKFYKMIKEGFRMLSPEHAPAEMYDIMKTCWDADPLKRPTFKQIVQLIEKQISESTNHIYSNLANCSPHRENPAVDHSVRINSVGSSASSTQPLLVHEDV

Target ORF information:

RefSeq Version	XM_005207937.4
Organism	Bos taurus(cattle)
Definition	Bos taurus KIT proto-oncogene receptor tyrosine kinase (KIT), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005207937.4

ORF Insert Sequence:

ATGAGAGGCG CTCGCGGCGC CTGGGATTTC CTCTTCGTTC TGCTGCTCCT GCTCCTCGTC 
CAGACAGGCT CTTCTCAGCC ATCTGTGAGT CCAGGGGAAC TGTCTCTACC ATCTATCCAC 
CCAGCAAAAT CAGAGTTAAT TGTCAGCGTT GGCGACGAGA TTAGGCTGTT ATGCACCGAT 
CCAGGATTTG TCAAGTGGAC TTTTGAGATC CTGGGTCAAC TGAGTGAGAA AACAAACCCG 
GAATGGATCA CCGAGAAAGC AGAGGCCACA AATACAGGCA ATTACACGTG CACCAATAAA 
GGCGGCTTGA GCAGTTCCAT TTATGTGTTT GTTAGAGACC CCGAGAAGCT TTTCCTGATT 
GACCTTCCCT TGTACGGGAA AGAAGAAAAC GACACGCTGG TTCGCTGTCC CCTGACAGAC 
CCCGAGGTGA CCAATTACTC TCTTACGGGG TGTGAGGGGA AACCTCTCCC CAAGGATTTG 
ACATTTGTGG CCGACCCCAA GGCAGGTATC ACAATCAGAA ATGTGAAGCG CGAGTACCAT 
CGGCTCTGTC TGCACTGCTC AGCGAATCAG AGGGGCAAGT CCATGCTGTC GAAGAAATTC 
ACTCTGAAAG TGCGGGCAGC CATCAAAGCT GTGCCAGTTG TGTCTGTGTC CAAAACCAGC 
TATCTTCTCA GGGAAGGAGA GGAATTTGCA GTGACATGCT TGATTAAAGA CGTGTCTAGT 
TCCGTGGACT CTATGTGGAT AAAGGAAAAC AGCCAGCAGA CTAAAGCACA GATGAAGAAG 
AATAGCTGGC ATCAGGGTGA CTTCAGTTAT CTCCGTCAGG AAAGGTTGAC TATCAGCTCA 
GCAAGAGTGA ATGATTCCGG TGTGTTCATG TGTTACGCCA ATAACACTTT TGGATCAGCA 
AATGTCACAA CAACCTTAGA AGTAGTAGAT AAAGGATTCA TTAATATCTT CCCTATGATG 
AACACAACAG TATTTGTAAA TGATGGAGAG AATGTGGATC TGGTTGTTGA ATATGAGGCA 
TATCCCAAAC CTGTACACCG ACAATGGATA TATATGAACA GAACCTCCAC TGATAAGTGG 
GACGATTATC CTAAGTCTGA AAATGAAAGT AATATCAGAT ACGTAAATGA ACTTCATCTA 
ACCAGATTAA AAGGGACTGA AGGAGGCACT TACACATTTC ACGTGTCCAA TTCTGATGTC 
AATTCTTCCG TGACATTTAA TGTTTACGTG AACACAAAAC CAGAAATCCT GACGCATGAC 
AGGCTGGTGA ATGGCATGCT ACAGTGCGTG GCCGCAGGGT TCCCGGAGCC AACCATCGAT 
TGGTACTTTT GTCCAGGAAC TGAGCAGAGG TGTTCTGTTC CCGTTGGGCC AGTGGATGTA 
CAGATCCAAA ACTCATCTGT CTCACCATTT GGAAAACTAG TGGTTTATAG CACCATTGAT 
GACAGCACAT TCAAACACAA TGGGACGGTG GAGTGCAGGG CTTATAACGA TGTGGGCAAG 
AGTTCTGCCT CTTTTAACTT TGCATTTAAA GAACAAATCC ATGCTCACAC CCTGTTCACG 
CCGTTGCTGA TTGGTTTTGT GATCGCAGCT GGTTTAATGT GTATCTTCGT GATGATTCTT 
ACATACAAAT ATTTGCAGAA ACCCATGTAT GAAGTACAGT GGAAAGTTGT CGAGGAGATA 
AATGGAAACA ATTATGTTTA CATAGACCCA ACACAACTTC CTTATGATCA CAAATGGGAG 
TTTCCCAGGA ACAGGCTGAG TTTTGGGAAA ACCCTGGGTG CTGGCGCCTT CGGGAAAGTT 
GTTGAGGCCA CCGCTTATGG CTTAATTAAA TCAGATGCAG CCATGACTGT TGCTGTCAAG 
ATGCTCAAAC CAAGCGCCCA TTTAACCGAA CGAGAAGCCC TAATGTCTGA ACTCAAAGTC 
TTGAGTTACC TCGGTAATCA TATGAATATT GTGAATCTTC TGGGAGCGTG CACCATTGGA 
GGGCCCACCC TGGTCATTAC AGAATATTGT TGCTATGGTG ATCTTCTGAA TTTTTTGAGA 
AGAAAACGTG ATTCATTTAT TTGCTCAAAG CAGGAAGATC ACGCCGAAGT GGCGCTTTAT 
AAGAACCTTC TTCATTCAAA GGAGTCTTCC TGCAATGATA GTACTAATGA GTACATGGAC 
ATGAAACCTG GAGTTTCTTA TGTTGTACCA ACCAAGGCAG ACAAGAGGAG ATCTGCAAGA 
ATAGGTTCAT ACATAGAAAG AGACGTGACT CCTGCTATCA TGGAAGATGA TGAGCTGGCC 
CTGGACCTGG AGGACTTGCT GAGCTTTTCT TACCAGGTGG CAAAAGGCAT GGCGTTCCTT 
GCCTCAAAGA ATTGTATTCA TAGAGACTTG GCAGCCAGAA ATATCCTCCT TACTCATGGT 
CGAATCACAA AGATTTGTGA TTTTGGTCTA GCCAGAGACA TCAAGAATGA TTCTAATTAT 
GTGGTCAAAG GAAACGCTCG ACTCCCTGTG AAGTGGATGG CACCAGAGAG TATTTTCAAC 
TGTGTATACA CATTTGAAAG TGATGTCTGG TCCTATGGGA TTTTTCTGTG GGAGCTGTTC 
TCTTTAGGAA GCAGCCCCTA CCCTGGAATG CCAGTCGATT CTAAGTTCTA CAAGATGATC 
AAGGAAGGTT TCCGAATGCT CAGCCCCGAG CATGCACCTG CGGAAATGTA TGACATCATG 
AAGACCTGCT GGGATGCTGA TCCCTTGAAA AGGCCAACAT TTAAGCAGAT TGTGCAGCTG 
ATTGAGAAGC AGATCTCAGA GAGCACCAAT CATATTTATT CCAACTTAGC AAACTGCAGC 
CCCCACCGGG AGAACCCCGC CGTGGACCAT TCTGTGCGCA TCAACTCTGT GGGCAGCAGC 
GCCTCCTCCA CGCAGCCTCT GCTTGTCCAC GAAGATGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A complex structural variant at the KIT locus in cattle with the Pinzgauer spotting pattern.
Animal genetics50(5)423-429(2019 Oct)
Küttel L,Letko A,Häfliger IM,Signer-Hasler H,Joller S,Hirsbrunner G,Mészáros G,Sölkner J,Flury C,Leeb T,Drögemüller C

Ectopic position of duplicated KIT gene in African Nguni cattle, associated with color sidedness, confirms its shared ancestry with the Bos taurus lineage.
Animal genetics48(1)122-123(2017 Feb)
Szczerbal I,Robinson TJ,Duran A,Davey SC,Andersson M,Switonski M

Genetic heterogeneity at the bovine KIT gene in cattle breeds carrying different putative alleles at the spotting locus.
Animal genetics41(3)295-303(2010 Jun)
Fontanesi L,Tazzoli M,Russo V,Beever J

KIT variants in bovine ovarian cells and corpus luteum.
Growth factors (Chur, Switzerland)27(2)100-13(2009 Apr)
Koch D,Sakurai M,Hummitzsch K,Hermsdorf T,Erdmann S,Schwalbe S,Stolzenburg JU,Spanel-Borowski K,Ricken AM

Fibroblast growth factor 7 stimulates in vitro growth of oocytes originating from bovine early antral follicles.
Molecular reproduction and development75(12)1736-43(2008 Dec)
Cho JH,Itoh T,Sendai Y,Hoshi H

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KIT cDNA ORF clone, Bos taurus(cattle)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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