Kit cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Kit
Entrez Gene ID	64030
Full Name	KIT proto-oncogene receptor tyrosine kinase
General protein information	Preferred Names KIT proto-oncogene receptor tyrosine kinase Names mast/stem cell growth factor receptor Kit Kit oncogene c-kit receptor tyrosine kinase protein kinase v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 14 Map Location: 14p11
Summary	transmembrane receptor; deletion mutation is associated with anemia and deficiency in mast cells and melanocytes in the Ws mutant allele of rats [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_022264.1	NP_071600.1	mast/stem cell growth factor receptor Kit precursor
XM_006250909.3	XP_006250971.1	mast/stem cell growth factor receptor Kit isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	KIT	NP_000213.1
Bos taurus (cattle)	KIT	NP_001159956.1
Rattus norvegicus (Norway rat)	Kit	NP_071600.1
Pan troglodytes (chimpanzee)	KIT	XP_517285.3
Danio rerio (zebrafish)	kita	NP_571128.1
Danio rerio (zebrafish)	kitb	NP_001137390.1
Xenopus tropicalis (tropical clawed frog)	kit	NP_001135545.1
Macaca mulatta (Rhesus monkey)	KIT	XP_001089071.1
Gallus gallus (chicken)	KIT	NP_989692.1
Mus musculus (house mouse)	Kit	NP_001116205.1
Canis lupus familiaris (dog)	KIT	NP_001003181.1

Gene Ontology
Bibliography

Related articles in PubMed

Kidney-derived c-kit
Rangel EB, Gomes SA, Kanashiro-Takeuchi R, Saltzman RG, Wei C, Ruiz P, Reiser J, Hare JM
Scientific reports8(1)14723(2018 10)

Bone marrow mesenchymal stem cell-derived exosomal miR-21 protects C-kit+ cardiac stem cells from oxidative injury through the PTEN/PI3K/Akt axis.
Shi B, Wang Y, Zhao R, Long X, Deng W, Wang Z
PloS one13(2)e0191616(2018)

SDF1 gradient associates with the distribution of c-Kit+ cardiac cells in the heart.
Renko O, Tolonen AM, Rysä J, Magga J, Mustonen E, Ruskoaho H, Serpi R
Scientific reports8(1)1160(2018 01)

Cadmium exposure in newborn rats ovary induces developmental disorders of primordial follicles and the differential expression of SCF/c-kit gene.
Zhang W, Wu T, Zhang C, Luo L, Xie M, Huang H
Toxicology letters28020-28(2017 Oct)

A role of KIT receptor signaling for proliferation and differentiation of rat stem Leydig cells in vitro.
Liu S, Chen X, Wang Y, Li L, Wang G, Li X, Chen H, Guo J, Lin H, Lian QQ, Ge RS
Molecular and cellular endocrinology4441-8(2017 03)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Kit gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Kit cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05573	NM_022264.1 Latest version!	Rattus norvegicus KIT proto-oncogene receptor tyrosine kinase (Kit), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa36031	XM_006250909.3 Latest version!	Rattus norvegicus KIT proto-oncogene receptor tyrosine kinase (Kit), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Kit ( NM_022264.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_071600.1 Rattus norvegicus KIT proto-oncogene receptor tyrosine kinase (Kit), mRNA.

Price & Availability↑

CloneID	ORa05573
Clone ID Related Accession (Same CDS sequence)	NM_022264.1
Accession Version	NM_022264.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1572796800000
Organism	Rattus norvegicus(Norway rat)
Product	mast/stem cell growth factor receptor Kit precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from D12524.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D12524.1, EU247827.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGAGAGGCG CTCGCGGCGC CTGGGATCTG CTCTGCGTCC TGTTGGTCCT GCTCCGTGGC 
CAGACAGGGA CTTCTCAGCC ATCTGCGAGT CCAGGGGAGC CGTCTCCACC ATCCATCCAG 
CCGGCCCAGT CAGAGTTAAT AGTTGAAGCC GGGGACACCA TCAGGCTGAC GTGCACTGAC 
CCCGCCTTTG TCAAATGGAC TTTCGAGATC CTCGATGTAA GGATTGAGAA TAAGCAGAGC 
GAATGGATTC GAGAAAAAGC CGAGGCCACT CACACGGGCA AATACACGTG CGTCAGCGGC 
AGCGGCCTCA GGAGCTCTAT TTACGTGTTC GTTAGAGATC CTGCCGTACT TTTCCTGGTT 
GGCCTTCCCT TGTTTGGCAA AGAAGACAAC GACGCACTGG TCCGCTGCCC CCTGACAGAC 
CCACAGGTGT CCAATTACTC CCTCATTGAG TGTGATGGGA AATCTCTCCC CACGGACCTG 
AAGTTCGTCC CCAACCCCAA GGCTGGCATC ACCATCAAAA ACGTGAAGCG CGCCTACCAC 
CGGCTGTGCA TCCGGTGTGC TGCCCAGCGT GAGGGCAAAT GGATGCGGTC TGACAAATTC 
ACCCTCAAAG TGAGAGCAGC CATCAAAGCT ATCCCAGTGG TGTCTGTGCC CGAAACAAGT 
CATCTCCTTA AGGAAGGGGA CACATTTACG GTGATATGCA CCATAAAAGA CGTGTCTACA 
TCCGTGGACT CCATGTGGAT AAAGTTGAAC CCTCAGCCTC AGAGCAAAGC CCAGGTAAAG 
CGCAATAGCT GGCATCAGGG CGACTTCAAT TACGAACGCC AGGAGACGCT GACTATCAGC 
TCAGCAAGAG TTAACGATTC CGGAGTGTTC ATGTGTTATG CCAATAATAC TTTTGGATCA 
GCAAATGTCA CAACAACCTT GAAAGTAGTA GAAAAGGGAT TCATCAACAT CTTCCCTGTG 
AAGAACACTA CGGTATTTGT AACTGATGGG GAAAATGTAG ACTTGGTTGT TGAGTTCGAG 
GCCTACCCTA AACCTGAACA CCAGCAGTGG ATCTACATGA ACAGGACGCC TACTAACAGA 
GGGGAGGATT ATGTCAAATC CGACAACCAA AGCAACATCA GATATGTGAA CGAACTTCGC 
CTGACCAGAT TGAAAGGCAC AGAAGGAGGC ACTTACACCT TTCTGGTGTC CAACTCTGAT 
GTCAGTGCTT CCGTGACATT TGATGTTTAT GTGAACACAA AACCAGAAAT CCTGACATAT 
GACAGGCTCA TGAATGGCAG GCTCCAGTGT GTGGCGGCGG GATTCCCGGA GCCCACAATA 
GATTGGTATT TTTGTACAGG GGCAGAGCAA AGGTGTACCG TTCCTGTCCC GCCAGTAGAC 
GTACAGATCC AGAATGCGTC TGTGTCACCA TTTGGAAAAC TGGTGGTTCA GAGTTCCATA 
GACTCCAGCG TCTTCCGGCA CAACGGCACG GTGGAGTGTA AGGCCTCCAA CGCTGTGGGC 
AAGAGCTCTG CCTTCTTTAA CTTTGCATTT AAAGGTAACA GCAAAGAGCA AATCCAGCCC 
CACACCCTGT TCACGCCGCT GCTCATTGGC TTCGTGGTCA CAGCCGGCTT GATGGGGATC 
ATTGTGATGG TTCTTGCCTA CAAATATTTG CAGAAACCCA TGTATGAAGT ACAATGGAAG 
GTTGTCGAGG AGATAAATGG GAACAATTAT GTTTACATAG ACCCAACGCA GCTTCCTTAT 
GACCACAAAT GGGAGTTTCC CAGAAACAGG CTGAGTTTTG GAAAGACCTT GGGAGCTGGT 
GCCTTTGGGA AGGTAGTTGA GGCCACTGCC TATGGCTTAA TTAAGTCGGA TGCCGCCATG 
ACGGTTGCCG TGAAGATGCT CAAACCAAGT GCCCATTTAA CGGAAAGGGA GGCCCTAATG 
TCAGAACTGA AGGTCCTGAG CTACCTGGGT AATCACATGA ATATCGTCAA CCTCCTTGGA 
GCGTGTACCG TGGGAGGGCC CACCCTGGTC ATTACAGAAT ACTGTTGCTA TGGTGATCTT 
TTGAATTTCT TGAGAAGAAA GCGTGACTCG TTTATTTTCT CAAAGCAAGA AGAACAGGCA 
GACGCCGCAC TTTATAAGAA CCTTCTGCAT TCAAAGGAGT CTTCCTGTGA CAGCTCAAAC 
GAGTACATGG ACATGAAGCC TGGCGTTTCC TACGTCGTAC CAACCAAGAC AGACAAAAGG 
AGATCCGCAA GAATAGACTC GTATATAGAA AGAGACGTGA CTCCCGCCAT CATGGAAGAT 
GACGAGCTGG CTCTGGACCT GGAAGATTTG CTGAGCTTTT CCTACCAGGT GGCCAAGGGC 
ATGGCGTTCC TCGCCTCCAA GAACTGTATT CACAGAGATT TGGCAGCCAG GAATATCCTC 
CTCACTCACG GGCGGATCAC AAAGATTTGC GATTTCGGCC TAGCCAGAGA CATCAGGAAT 
GATTCGAATT ACGTGGTAAA AGGAAATGCA CGGCTGCCCG TGAAGTGGAT GGCACCGGAG 
AGCATTTTCA ACTGCGTGTA CACATTTGAA AGTGACGTCT GGTCCTATGG GATTTTCCTC 
TGGGAGCTAT TCTCTCTAGG AAGCAGCCCC TACCCAGGGA TGCCGGTCGA TTCCAAGTTT 
TACAAGATGA TCAAGGAAGG TTTCCGAATG CTCAGCCCTG AGCACGCGCC TGCCGCAATG 
TATGAAGTTA TGAAGACTTG CTGGGATGCT GATCCCCTGA AAAGGCCAAC ATTCAAGCAG 
GTTGTTCAGC TCATTGAGAA GCAGATCTCA GACAGCAGCA AACATATTTA CTCCAACTTA 
GCAAACTGTA ACCCCAACCC AGAGAACCCC GTGGTGGTGG ACCATTCTGT GAGGGTCAAT 
TCCGTCGGCA GCAGCACCTC TTCCACACAG CCTCTCCTCG TGCATGAGGA CGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071600.1
CDS	45..2981
Translation	MRGARGAWDLLCVLLVLLRGQTGTSQPSASPGEPSPPSIQPAQSELIVEAGDTIRLTCTDPAFVKWTFEILDVRIENKQSEWIREKAEATHTGKYTCVSGSGLRSSIYVFVRDPAVLFLVGLPLFGKEDNDALVRCPLTDPQVSNYSLIECDGKSLPTDLKFVPNPKAGITIKNVKRAYHRLCIRCAAQREGKWMRSDKFTLKVRAAIKAIPVVSVPETSHLLKEGDTFTVICTIKDVSTSVDSMWIKLNPQPQSKAQVKRNSWHQGDFNYERQETLTISSARVNDSGVFMCYANNTFGSANVTTTLKVVEKGFINIFPVKNTTVFVTDGENVDLVVEFEAYPKPEHQQWIYMNRTPTNRGEDYVKSDNQSNIRYVNELRLTRLKGTEGGTYTFLVSNSDVSASVTFDVYVNTKPEILTYDRLMNGRLQCVAAGFPEPTIDWYFCTGAEQRCTVPVPPVDVQIQNASVSPFGKLVVQSSIDSSVFRHNGTVECKASNAVGKSSAFFNFAFKGNSKEQIQPHTLFTPLLIGFVVTAGLMGIIVMVLAYKYLQKPMYEVQWKVVEEINGNNYVYIDPTQLPYDHKWEFPRNRLSFGKTLGAGAFGKVVEATAYGLIKSDAAMTVAVKMLKPSAHLTEREALMSELKVLSYLGNHMNIVNLLGACTVGGPTLVITEYCCYGDLLNFLRRKRDSFIFSKQEEQADAALYKNLLHSKESSCDSSNEYMDMKPGVSYVVPTKTDKRRSARIDSYIERDVTPAIMEDDELALDLEDLLSFSYQVAKGMAFLASKNCIHRDLAARNILLTHGRITKICDFGLARDIRNDSNYVVKGNARLPVKWMAPESIFNCVYTFESDVWSYGIFLWELFSLGSSPYPGMPVDSKFYKMIKEGFRMLSPEHAPAAMYEVMKTCWDADPLKRPTFKQVVQLIEKQISDSSKHIYSNLANCNPNPENPVVVDHSVRVNSVGSSTSSTQPLLVHEDA

Target ORF information:

RefSeq Version	NM_022264.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus KIT proto-oncogene receptor tyrosine kinase (Kit), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022264.1

ORF Insert Sequence:

ATGAGAGGCG CTCGCGGCGC CTGGGATCTG CTCTGCGTCC TGTTGGTCCT GCTCCGTGGC 
CAGACAGGGA CTTCTCAGCC ATCTGCGAGT CCAGGGGAGC CGTCTCCACC ATCCATCCAG 
CCGGCCCAGT CAGAGTTAAT AGTTGAAGCC GGGGACACCA TCAGGCTGAC GTGCACTGAC 
CCCGCCTTTG TCAAATGGAC TTTCGAGATC CTCGATGTAA GGATTGAGAA TAAGCAGAGC 
GAATGGATTC GAGAAAAAGC CGAGGCCACT CACACGGGCA AATACACGTG CGTCAGCGGC 
AGCGGCCTCA GGAGCTCTAT TTACGTGTTC GTTAGAGATC CTGCCGTACT TTTCCTGGTT 
GGCCTTCCCT TGTTTGGCAA AGAAGACAAC GACGCACTGG TCCGCTGCCC CCTGACAGAC 
CCACAGGTGT CCAATTACTC CCTCATTGAG TGTGATGGGA AATCTCTCCC CACGGACCTG 
AAGTTCGTCC CCAACCCCAA GGCTGGCATC ACCATCAAAA ACGTGAAGCG CGCCTACCAC 
CGGCTGTGCA TCCGGTGTGC TGCCCAGCGT GAGGGCAAAT GGATGCGGTC TGACAAATTC 
ACCCTCAAAG TGAGAGCAGC CATCAAAGCT ATCCCAGTGG TGTCTGTGCC CGAAACAAGT 
CATCTCCTTA AGGAAGGGGA CACATTTACG GTGATATGCA CCATAAAAGA CGTGTCTACA 
TCCGTGGACT CCATGTGGAT AAAGTTGAAC CCTCAGCCTC AGAGCAAAGC CCAGGTAAAG 
CGCAATAGCT GGCATCAGGG CGACTTCAAT TACGAACGCC AGGAGACGCT GACTATCAGC 
TCAGCAAGAG TTAACGATTC CGGAGTGTTC ATGTGTTATG CCAATAATAC TTTTGGATCA 
GCAAATGTCA CAACAACCTT GAAAGTAGTA GAAAAGGGAT TCATCAACAT CTTCCCTGTG 
AAGAACACTA CGGTATTTGT AACTGATGGG GAAAATGTAG ACTTGGTTGT TGAGTTCGAG 
GCCTACCCTA AACCTGAACA CCAGCAGTGG ATCTACATGA ACAGGACGCC TACTAACAGA 
GGGGAGGATT ATGTCAAATC CGACAACCAA AGCAACATCA GATATGTGAA CGAACTTCGC 
CTGACCAGAT TGAAAGGCAC AGAAGGAGGC ACTTACACCT TTCTGGTGTC CAACTCTGAT 
GTCAGTGCTT CCGTGACATT TGATGTTTAT GTGAACACAA AACCAGAAAT CCTGACATAT 
GACAGGCTCA TGAATGGCAG GCTCCAGTGT GTGGCGGCGG GATTCCCGGA GCCCACAATA 
GATTGGTATT TTTGTACAGG GGCAGAGCAA AGGTGTACCG TTCCTGTCCC GCCAGTAGAC 
GTACAGATCC AGAATGCGTC TGTGTCACCA TTTGGAAAAC TGGTGGTTCA GAGTTCCATA 
GACTCCAGCG TCTTCCGGCA CAACGGCACG GTGGAGTGTA AGGCCTCCAA CGCTGTGGGC 
AAGAGCTCTG CCTTCTTTAA CTTTGCATTT AAAGGTAACA GCAAAGAGCA AATCCAGCCC 
CACACCCTGT TCACGCCGCT GCTCATTGGC TTCGTGGTCA CAGCCGGCTT GATGGGGATC 
ATTGTGATGG TTCTTGCCTA CAAATATTTG CAGAAACCCA TGTATGAAGT ACAATGGAAG 
GTTGTCGAGG AGATAAATGG GAACAATTAT GTTTACATAG ACCCAACGCA GCTTCCTTAT 
GACCACAAAT GGGAGTTTCC CAGAAACAGG CTGAGTTTTG GAAAGACCTT GGGAGCTGGT 
GCCTTTGGGA AGGTAGTTGA GGCCACTGCC TATGGCTTAA TTAAGTCGGA TGCCGCCATG 
ACGGTTGCCG TGAAGATGCT CAAACCAAGT GCCCATTTAA CGGAAAGGGA GGCCCTAATG 
TCAGAACTGA AGGTCCTGAG CTACCTGGGT AATCACATGA ATATCGTCAA CCTCCTTGGA 
GCGTGTACCG TGGGAGGGCC CACCCTGGTC ATTACAGAAT ACTGTTGCTA TGGTGATCTT 
TTGAATTTCT TGAGAAGAAA GCGTGACTCG TTTATTTTCT CAAAGCAAGA AGAACAGGCA 
GACGCCGCAC TTTATAAGAA CCTTCTGCAT TCAAAGGAGT CTTCCTGTGA CAGCTCAAAC 
GAGTACATGG ACATGAAGCC TGGCGTTTCC TACGTCGTAC CAACCAAGAC AGACAAAAGG 
AGATCCGCAA GAATAGACTC GTATATAGAA AGAGACGTGA CTCCCGCCAT CATGGAAGAT 
GACGAGCTGG CTCTGGACCT GGAAGATTTG CTGAGCTTTT CCTACCAGGT GGCCAAGGGC 
ATGGCGTTCC TCGCCTCCAA GAACTGTATT CACAGAGATT TGGCAGCCAG GAATATCCTC 
CTCACTCACG GGCGGATCAC AAAGATTTGC GATTTCGGCC TAGCCAGAGA CATCAGGAAT 
GATTCGAATT ACGTGGTAAA AGGAAATGCA CGGCTGCCCG TGAAGTGGAT GGCACCGGAG 
AGCATTTTCA ACTGCGTGTA CACATTTGAA AGTGACGTCT GGTCCTATGG GATTTTCCTC 
TGGGAGCTAT TCTCTCTAGG AAGCAGCCCC TACCCAGGGA TGCCGGTCGA TTCCAAGTTT 
TACAAGATGA TCAAGGAAGG TTTCCGAATG CTCAGCCCTG AGCACGCGCC TGCCGCAATG 
TATGAAGTTA TGAAGACTTG CTGGGATGCT GATCCCCTGA AAAGGCCAAC ATTCAAGCAG 
GTTGTTCAGC TCATTGAGAA GCAGATCTCA GACAGCAGCA AACATATTTA CTCCAACTTA 
GCAAACTGTA ACCCCAACCC AGAGAACCCC GTGGTGGTGG ACCATTCTGT GAGGGTCAAT 
TCCGTCGGCA GCAGCACCTC TTCCACACAG CCTCTCCTCG TGCATGAGGA CGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kit ( XM_006250909.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006250971.1 Rattus norvegicus KIT proto-oncogene receptor tyrosine kinase (Kit), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa36031
Clone ID Related Accession (Same CDS sequence)	XM_006250909.3
Accession Version	XM_006250909.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2925bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	mast/stem cell growth factor receptor Kit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006250909.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGGCG CTCGCGGCGC CTGGGATCTG CTCTGCGTCC TGTTGGTCCT GCTCCGTGGC 
CAGACAGGGA CTTCTCAGCC ATCTGCGAGT CCAGGGGAGC CGTCTCCACC ATCCATCCAG 
CCGGCCCAGT CAGAGTTAAT AGTTGAAGCC GGCGACACCA TCAGGCTGAC GTGCACTGAC 
CCCGCCTTTG TCAAATGGAC TTTCGAGATC CTCGATGTAA GGATTGAGAA TAAGCAGAGC 
GAATGGATTC GAGAAAAAGC CGAGGCCACT CACACGGGCA AATACACGTG CGTCAGCGGC 
AGCGGCCTCA GGAGCTCTAT TTACGTGTTC GTTAGAGATC CTGCCGTACT TTTCCTGGTT 
GGCCTTCCCT TGTTTGGCAA AGAAGACAAT GACGCACTGG TCCGCTGCCC CCTGACAGAC 
CCACAGGTGT CCAATTACTC CCTCATTGAG TGTGATGGGA AATCTCTCCC CACGGACCTG 
AAGTTCGTCC CCAACCCCAA GGCTGGCATC ACCATCAAAA ACGTGAAGCG CGCCTACCAC 
CGGCTGTGCA TCCGGTGTGC TGCCCAGCGT GAGGGCAAAT GGATGCGGTC TGACAAATTC 
ACCCTCAAAG TGAGGGCAGC CATCAAAGCT ATCCCAGTGG TGTCTGTGCC CGAAACAAGT 
CATCTCCTTA AGGAAGGGGA CACATTTACG GTGATATGCA CCATAAAAGA CGTGTCTACA 
TCCGTGGACT CCATGTGGAT AAAGTTGAAC CCTCAGCCTC AGAGCAAAGC CCAGGTAAAG 
CGCAATAGCT GGCATCAGGG CGACTTCAAT TACGAACGCC AGGAGACGCT GACTATCAGC 
TCAGCAAGAG TTAACGATTC CGGAGTGTTC ATGTGTTATG CCAATAATAC TTTTGGATCA 
GCAAATGTCA CAACAACCTT GAAAGTAGTA GAAAAGGGAT TCATCAACAT CTTCCCTGTG 
AAGAACACTA CGGTATTTGT AACTGATGGG GAAAATGTAG ACTTGGTTGT TGAGTTCGAG 
GCCTACCCTA AACCTGAACA CCAGCAGTGG ATCTACATGA ACAGGACGCC TACTAACAGA 
GGGGAGGATT ATGTCAAATC CGACAACCAA AGCAACATCA GATATGTGAA CGAACTTCGC 
CTGACCAGAT TGAAAGGCAC AGAAGGAGGC ACTTACACCT TTCTGGTGTC CAACTCTGAT 
GTCAGTGCTT CCGTGACATT TGATGTTTAT GTGAACACAA AACCAGAAAT CCTGACATAT 
GACAGGCTCA TGAATGGCAG GCTCCAGTGT GTGGCGGCGG GATTCCCGGA GCCCACAATA 
GATTGGTATT TTTGTACAGG GGCAGAGCAA AGGTGTACCG TTCCTGTCCC GCCAGTAGAC 
GTACAGATCC AGAATGCGTC TGTGTCACCA TTTGGAAAAC TGGTGGTTCA GAGTTCCATA 
GACTCCAGCG TCTTCCGGCA CAACGGCACG GTGGAGTGTA AGGCCTCCAA CGCTGTGGGC 
AAGAGCTCTG CCTTCTTTAA CTTTGCATTT AAAGAGCAAA TCCAGCCCCA CACCCTGTTC 
ACGCCGCTGC TCATTGGCTT CGTGGTCACA GCCGGCTTGA TGGGGATCAT TGTGATGGTT 
CTTGCCTACA AATATTTGCA GAAACCCATG TATGAAGTAC AATGGAAGGT TGTCGAGGAG 
ATAAATGGGA ACAATTATGT TTACATAGAC CCAACGCAGC TTCCTTATGA CCACAAATGG 
GAGTTTCCCA GAAACAGGCT GAGTTTTGGA AAGACCTTGG GAGCTGGTGC CTTTGGGAAG 
GTAGTTGAGG CCACTGCCTA TGGCTTAATT AAGTCGGATG CCGCCATGAC GGTTGCCGTG 
AAGATGCTCA AACCAAGTGC CCATTTAACG GAAAGGGAGG CCCTAATGTC AGAACTGAAG 
GTCCTGAGCT ACCTGGGTAA TCACATGAAT ATCGTCAACC TCCTTGGAGC GTGTACCGTG 
GGAGGGCCCA CCCTGGTCAT TACAGAATAC TGTTGCTATG GTGATCTTTT GAATTTCTTG 
AGAAGAAAGC GTGACTCGTT TATTTTCTCA AAGCAAGAAG AACAGGCAGA CGCCGCACTT 
TATAAGAACC TTCTGCATTC AAAGGAGTCT TCCTGTGACA GCTCAAACGA GTACATGGAC 
ATGAAGCCTG GCGTTTCCTA CGTCGTACCA ACCAAGACAG ACAAAAGGAG ATCCGCAAGA 
ATAGACTCGT ATATAGAAAG AGACGTGACT CCCGCCATCA TGGAAGATGA CGAGCTGGCT 
CTGGACCTGG AAGATTTGCT GAGCTTTTCC TACCAGGTGG CCAAGGGCAT GGCGTTCCTC 
GCCTCCAAGA ACTGTATTCA CAGAGATTTG GCAGCCAGGA ATATCCTCCT CACTCACGGG 
CGGATCACAA AGATTTGCGA TTTCGGCCTA GCCAGAGACA TCAGGAATGA TTCGAATTAC 
GTGGTAAAAG GAAATGCACG GCTGCCCGTG AAGTGGATGG CACCGGAGAG CATTTTCAAC 
TGCGTGTACA CATTTGAAAG TGACGTCTGG TCCTATGGGA TTTTCCTCTG GGAGCTATTC 
TCTCTAGGAA GCAGCCCCTA CCCAGGGATG CCGGTCGATT CCAAGTTTTA CAAGATGATC 
AAGGAAGGTT TCCGAATGCT CAGCCCTGAG CACGCGCCTG CCGCAATGTA TGAAGTTATG 
AAGACTTGCT GGGATGCTGA TCCCCTGAAA AGGCCAACAT TCAAGCAGGT TGTTCAGCTC 
ATTGAGAAGC AGATCTCAGA CAGCAGCAAA CATATTTACT CCAACTTAGC AAACTGTAAC 
CCCAACCCAG AGAACCCCGT GGTGGTGGAC CATTCTGTGA GGGTCAATTC CGTCGGCAGC 
AGCACCTCTT CCACACAGCC TCTCCTTGTG CATGAGGACG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006250971.1
CDS	75..2999
Translation	MRGARGAWDLLCVLLVLLRGQTGTSQPSASPGEPSPPSIQPAQSELIVEAGDTIRLTCTDPAFVKWTFEILDVRIENKQSEWIREKAEATHTGKYTCVSGSGLRSSIYVFVRDPAVLFLVGLPLFGKEDNDALVRCPLTDPQVSNYSLIECDGKSLPTDLKFVPNPKAGITIKNVKRAYHRLCIRCAAQREGKWMRSDKFTLKVRAAIKAIPVVSVPETSHLLKEGDTFTVICTIKDVSTSVDSMWIKLNPQPQSKAQVKRNSWHQGDFNYERQETLTISSARVNDSGVFMCYANNTFGSANVTTTLKVVEKGFINIFPVKNTTVFVTDGENVDLVVEFEAYPKPEHQQWIYMNRTPTNRGEDYVKSDNQSNIRYVNELRLTRLKGTEGGTYTFLVSNSDVSASVTFDVYVNTKPEILTYDRLMNGRLQCVAAGFPEPTIDWYFCTGAEQRCTVPVPPVDVQIQNASVSPFGKLVVQSSIDSSVFRHNGTVECKASNAVGKSSAFFNFAFKEQIQPHTLFTPLLIGFVVTAGLMGIIVMVLAYKYLQKPMYEVQWKVVEEINGNNYVYIDPTQLPYDHKWEFPRNRLSFGKTLGAGAFGKVVEATAYGLIKSDAAMTVAVKMLKPSAHLTEREALMSELKVLSYLGNHMNIVNLLGACTVGGPTLVITEYCCYGDLLNFLRRKRDSFIFSKQEEQADAALYKNLLHSKESSCDSSNEYMDMKPGVSYVVPTKTDKRRSARIDSYIERDVTPAIMEDDELALDLEDLLSFSYQVAKGMAFLASKNCIHRDLAARNILLTHGRITKICDFGLARDIRNDSNYVVKGNARLPVKWMAPESIFNCVYTFESDVWSYGIFLWELFSLGSSPYPGMPVDSKFYKMIKEGFRMLSPEHAPAAMYEVMKTCWDADPLKRPTFKQVVQLIEKQISDSSKHIYSNLANCNPNPENPVVVDHSVRVNSVGSSTSSTQPLLVHEDA

Target ORF information:

RefSeq Version	XM_006250909.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus KIT proto-oncogene receptor tyrosine kinase (Kit), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006250909.3

ORF Insert Sequence:

ATGAGAGGCG CTCGCGGCGC CTGGGATCTG CTCTGCGTCC TGTTGGTCCT GCTCCGTGGC 
CAGACAGGGA CTTCTCAGCC ATCTGCGAGT CCAGGGGAGC CGTCTCCACC ATCCATCCAG 
CCGGCCCAGT CAGAGTTAAT AGTTGAAGCC GGCGACACCA TCAGGCTGAC GTGCACTGAC 
CCCGCCTTTG TCAAATGGAC TTTCGAGATC CTCGATGTAA GGATTGAGAA TAAGCAGAGC 
GAATGGATTC GAGAAAAAGC CGAGGCCACT CACACGGGCA AATACACGTG CGTCAGCGGC 
AGCGGCCTCA GGAGCTCTAT TTACGTGTTC GTTAGAGATC CTGCCGTACT TTTCCTGGTT 
GGCCTTCCCT TGTTTGGCAA AGAAGACAAT GACGCACTGG TCCGCTGCCC CCTGACAGAC 
CCACAGGTGT CCAATTACTC CCTCATTGAG TGTGATGGGA AATCTCTCCC CACGGACCTG 
AAGTTCGTCC CCAACCCCAA GGCTGGCATC ACCATCAAAA ACGTGAAGCG CGCCTACCAC 
CGGCTGTGCA TCCGGTGTGC TGCCCAGCGT GAGGGCAAAT GGATGCGGTC TGACAAATTC 
ACCCTCAAAG TGAGGGCAGC CATCAAAGCT ATCCCAGTGG TGTCTGTGCC CGAAACAAGT 
CATCTCCTTA AGGAAGGGGA CACATTTACG GTGATATGCA CCATAAAAGA CGTGTCTACA 
TCCGTGGACT CCATGTGGAT AAAGTTGAAC CCTCAGCCTC AGAGCAAAGC CCAGGTAAAG 
CGCAATAGCT GGCATCAGGG CGACTTCAAT TACGAACGCC AGGAGACGCT GACTATCAGC 
TCAGCAAGAG TTAACGATTC CGGAGTGTTC ATGTGTTATG CCAATAATAC TTTTGGATCA 
GCAAATGTCA CAACAACCTT GAAAGTAGTA GAAAAGGGAT TCATCAACAT CTTCCCTGTG 
AAGAACACTA CGGTATTTGT AACTGATGGG GAAAATGTAG ACTTGGTTGT TGAGTTCGAG 
GCCTACCCTA AACCTGAACA CCAGCAGTGG ATCTACATGA ACAGGACGCC TACTAACAGA 
GGGGAGGATT ATGTCAAATC CGACAACCAA AGCAACATCA GATATGTGAA CGAACTTCGC 
CTGACCAGAT TGAAAGGCAC AGAAGGAGGC ACTTACACCT TTCTGGTGTC CAACTCTGAT 
GTCAGTGCTT CCGTGACATT TGATGTTTAT GTGAACACAA AACCAGAAAT CCTGACATAT 
GACAGGCTCA TGAATGGCAG GCTCCAGTGT GTGGCGGCGG GATTCCCGGA GCCCACAATA 
GATTGGTATT TTTGTACAGG GGCAGAGCAA AGGTGTACCG TTCCTGTCCC GCCAGTAGAC 
GTACAGATCC AGAATGCGTC TGTGTCACCA TTTGGAAAAC TGGTGGTTCA GAGTTCCATA 
GACTCCAGCG TCTTCCGGCA CAACGGCACG GTGGAGTGTA AGGCCTCCAA CGCTGTGGGC 
AAGAGCTCTG CCTTCTTTAA CTTTGCATTT AAAGAGCAAA TCCAGCCCCA CACCCTGTTC 
ACGCCGCTGC TCATTGGCTT CGTGGTCACA GCCGGCTTGA TGGGGATCAT TGTGATGGTT 
CTTGCCTACA AATATTTGCA GAAACCCATG TATGAAGTAC AATGGAAGGT TGTCGAGGAG 
ATAAATGGGA ACAATTATGT TTACATAGAC CCAACGCAGC TTCCTTATGA CCACAAATGG 
GAGTTTCCCA GAAACAGGCT GAGTTTTGGA AAGACCTTGG GAGCTGGTGC CTTTGGGAAG 
GTAGTTGAGG CCACTGCCTA TGGCTTAATT AAGTCGGATG CCGCCATGAC GGTTGCCGTG 
AAGATGCTCA AACCAAGTGC CCATTTAACG GAAAGGGAGG CCCTAATGTC AGAACTGAAG 
GTCCTGAGCT ACCTGGGTAA TCACATGAAT ATCGTCAACC TCCTTGGAGC GTGTACCGTG 
GGAGGGCCCA CCCTGGTCAT TACAGAATAC TGTTGCTATG GTGATCTTTT GAATTTCTTG 
AGAAGAAAGC GTGACTCGTT TATTTTCTCA AAGCAAGAAG AACAGGCAGA CGCCGCACTT 
TATAAGAACC TTCTGCATTC AAAGGAGTCT TCCTGTGACA GCTCAAACGA GTACATGGAC 
ATGAAGCCTG GCGTTTCCTA CGTCGTACCA ACCAAGACAG ACAAAAGGAG ATCCGCAAGA 
ATAGACTCGT ATATAGAAAG AGACGTGACT CCCGCCATCA TGGAAGATGA CGAGCTGGCT 
CTGGACCTGG AAGATTTGCT GAGCTTTTCC TACCAGGTGG CCAAGGGCAT GGCGTTCCTC 
GCCTCCAAGA ACTGTATTCA CAGAGATTTG GCAGCCAGGA ATATCCTCCT CACTCACGGG 
CGGATCACAA AGATTTGCGA TTTCGGCCTA GCCAGAGACA TCAGGAATGA TTCGAATTAC 
GTGGTAAAAG GAAATGCACG GCTGCCCGTG AAGTGGATGG CACCGGAGAG CATTTTCAAC 
TGCGTGTACA CATTTGAAAG TGACGTCTGG TCCTATGGGA TTTTCCTCTG GGAGCTATTC 
TCTCTAGGAA GCAGCCCCTA CCCAGGGATG CCGGTCGATT CCAAGTTTTA CAAGATGATC 
AAGGAAGGTT TCCGAATGCT CAGCCCTGAG CACGCGCCTG CCGCAATGTA TGAAGTTATG 
AAGACTTGCT GGGATGCTGA TCCCCTGAAA AGGCCAACAT TCAAGCAGGT TGTTCAGCTC 
ATTGAGAAGC AGATCTCAGA CAGCAGCAAA CATATTTACT CCAACTTAGC AAACTGTAAC 
CCCAACCCAG AGAACCCCGT GGTGGTGGAC CATTCTGTGA GGGTCAATTC CGTCGGCAGC 
AGCACCTCTT CCACACAGCC TCTCCTTGTG CATGAGGACG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Kidney-derived c-kit
Scientific reports8(1)14723(2018 10)
Rangel EB,Gomes SA,Kanashiro-Takeuchi R,Saltzman RG,Wei C,Ruiz P,Reiser J,Hare JM

Bone marrow mesenchymal stem cell-derived exosomal miR-21 protects C-kit+ cardiac stem cells from oxidative injury through the PTEN/PI3K/Akt axis.
PloS one13(2)e0191616(2018)
Shi B,Wang Y,Zhao R,Long X,Deng W,Wang Z

SDF1 gradient associates with the distribution of c-Kit+ cardiac cells in the heart.
Scientific reports8(1)1160(2018 01)
Renko O,Tolonen AM,Rysä J,Magga J,Mustonen E,Ruskoaho H,Serpi R

Cadmium exposure in newborn rats ovary induces developmental disorders of primordial follicles and the differential expression of SCF/c-kit gene.
Toxicology letters28020-28(2017 Oct)
Zhang W,Wu T,Zhang C,Luo L,Xie M,Huang H

A role of KIT receptor signaling for proliferation and differentiation of rat stem Leydig cells in vitro.
Molecular and cellular endocrinology4441-8(2017 03)
Liu S,Chen X,Wang Y,Li L,Wang G,Li X,Chen H,Guo J,Lin H,Lian QQ,Ge RS

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Kit cDNA ORF clone, Rattus norvegicus(Norway rat)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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