kcnt1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	kcnt1
Entrez Gene ID	779818
Full Name	potassium channel, sodium activated subfamily T, member 1
Synonyms	KCa4.1,Slo2.2,slack
General protein information	Preferred Names potassium channel, sodium activated subfamily T, member 1 Names potassium channel subfamily T member 2 potassium channel, sodium activated subfamily T, member 1 potassium channel, subfamily T, member 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012953117.3	XP_012808571.1	potassium channel subfamily T member 2 isoform X1
XM_012953119.3	XP_012808573.1	potassium channel subfamily T member 2 isoform X2
XM_031899402.1	XP_031755262.1	potassium channel subfamily T member 2 isoform X3
XM_012953121.2	XP_012808575.1	potassium channel subfamily T member 2 isoform X3
XM_012953118.2	XP_012808572.1	potassium channel subfamily T member 2 isoform X1
XM_012953122.2	XP_012808576.1	potassium channel subfamily T member 2 isoform X4
XM_012953117.2	XP_012808571.1	potassium channel subfamily T member 2 isoform X1
XM_012953119.2	XP_012808573.1	potassium channel subfamily T member 2 isoform X2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Kcnt2	NP_942057.1
Canis lupus familiaris (dog)	KCNT2	XP_849284.2
Danio rerio (zebrafish)	LOC565691	XP_005164308.1
Pan troglodytes (chimpanzee)	KCNT2	XP_001134868.1
Macaca mulatta (Rhesus monkey)	KCNT2	XP_001112100.1
Bos taurus (cattle)	KCNT2	XP_003587097.2
Homo sapiens (human)	KCNT2	NP_940905.2
Mus musculus (house mouse)	Kcnt2	NP_001074496.2
Gallus gallus (chicken)	KCNT2	XP_426614.3
Xenopus tropicalis (tropical clawed frog)	kcnt1	XP_004918922.1

Gene Ontology

The following kcnt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the kcnt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa29213	XM_012953122.2 Latest version!	Xenopus tropicalis potassium channel, sodium activated subfamily T, member 1 (kcnt1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa29209	XM_012953117.3 Latest version!	Xenopus tropicalis potassium channel, sodium activated subfamily T, member 2 like (kcnt2l), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa29209	XM_012953118.2 Latest version!	Xenopus tropicalis potassium channel, sodium activated subfamily T, member 1 (kcnt1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa29210	XM_012953119.3 Latest version!	Xenopus tropicalis potassium channel, sodium activated subfamily T, member 2 like (kcnt2l), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa29211	XM_031899402.1 Latest version!	Xenopus tropicalis potassium channel, sodium activated subfamily T, member 2 like (kcnt2l), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa29212	XM_012953121.2 Latest version!	Xenopus tropicalis potassium channel, sodium activated subfamily T, member 1 (kcnt1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OXa29209	XM_012953117.2	Xenopus tropicalis potassium channel, sodium activated subfamily T, member 1 (kcnt1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa29210	XM_012953119.2	Xenopus tropicalis potassium channel, sodium activated subfamily T, member 1 (kcnt1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

kcnt1 ( XM_012953122.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012808576.1 Xenopus tropicalis potassium channel, sodium activated subfamily T, member 1 (kcnt1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OXa29213
Clone ID Related Accession (Same CDS sequence)	XM_012953122.2
Accession Version	XM_012953122.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2868bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	potassium channel subfamily T member 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012953122.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGG AGAAAGATGT GCAACCTTTG CCTCCACGCT ATCGATTTCG GGACTTGTTG 
ATGGGTGACT GGCAACAGGA TGACAGGGTC CATGTGGAAT TTTATGTCAA TGAAAACTCG 
TTTAAGGAAC GACTAAAGTT ATTTTTTATA AAGAATCAGA GATCAAGTTT GAGAGTCAGA 
GTCTTTAACT GTTCCTTAAA GTTACTGAGT TGCCTCCTCT ATATTATAAG GGTCCTTCTG 
GATGATCCGA GAGAGAGCAG GGGCGGATGC TGGGAATGTA CCAAACAGAA TTACTCTGCA 
CAACAGAAAT TTGACTGGAC CCCAATTATT TGGGTAAATA GACCTCTTCC CCTGTGGGGG 
CTTCAGGTAC TCGTGGCGCT AATTAGCTTC TGGGAAACAA TGCTTCTCAC CTACCTTGGC 
TACAAGGGAA ACATATGGGA GCAGCTCCTC CGGATCCCCT TCATTCTAGA GATGCTGAAC 
ACCCTGCCTT TCTTTGTCAC GATTTTCTGG CCTCCGTTGA GGAATGTCTT CATTCCTGTT 
TTCCTCAATT GCTGGTTGGC TAAACATGCG CTGGAGAACA TGATAAATGA CTTGCACCGA 
GCCATTCAGA GGACACACTC GGCCATGTTT AACCAGGTCT TTATTCTGAT ATGCACCCTC 
ATCTGCCTCA TGTTCACTTG CATCTGTGGG ATTCAGCATC TGGAGAGAGC GGGCAATAAC 
CTCACCCTCT TTGACTCCCT GTATTTCTGC ATTGTCACTT TCTCCACGGT GGGCTTCGGA 
GACGTCACCC CAAAGATCTG GCCGTCACAG CTCCTGGTTG TCATTATGAT CTGTGTGGCA 
CTCATTGTGC TGCCCATCCA GTTTGAGCAG CTCGTCTTCC TATGGATGGA AAGGCAGAAA 
TCTGGCGGGA ATTACAGCCG ATACCGAGCG CAGACGGAGA AGCACGTGGT CCTGTGCGTC 
AGTTCTTTGA AGATTGACCT GCTTATGGAT TTCCTCAATG AATTCTATGC TCATCCCCGG 
CTTCAGGACC ATTATGTTGT TCTCCTGTGC CCCACTGAGA TGGACCTGCC TGTGAGGAGA 
GTCCTGCAAA TTCCTCTCTG GTCCCAACGA GTCATCTATC TGCAGGGTTC TGCCCTCAAG 
GACCAGGATC TCATGAGAGC CAAAATTGAT GACGCAGAAG CCTGTTTTAT ACTCAGCAAT 
CGTTTCGAGC GAGACCGCAT CGCAGCTGAC CATCAGACCA TCCTCCGTGC CTGGGCCGTG 
AAGGACTTCG CACCAAACTG TCCCCTCTAC GTTCAGATAC TCAAACCGGA AAACAAGTTT 
CACATCAAAT TTGCAGATCA TGTGGTCTGT GAAGAAGAAT TCAAGTACGC CATGTTGGCT 
CTAAATTGCG TCTGCCCAGC GACTTCCACC CTGATAACTC TGCTCATCCA CACCTCTAGG 
GGGCAAGACA GTGCCAGTTC TCCGGATCAG TGGCAGAACA CCTACAGCCG CTGCTCGGCC 
AATGAAGTTT ATCACATCCG ACTGAGTGAA AGCAAATTCT TTGGAGAGTA TGAAGGGAAA 
AGCTTTACCT ATTCATCCTT CCACGCACAT AAAAAGTACG GGGTCTGTTT GATTGGTGTC 
CGTCGGGATA ACAAAACCAG CATCCTGCTG AATCCAGGGC CTTGCCATAT AATGTCCTCA 
ACAGATACCT GCTTCTACAT AAATATCTCA AAAGAAGAGA ACTCGGCATT CAGTTTGAAA 
GACAACGAAG AAGCCAAGAA ATCCGGGTAC CCAAAGTCTA CCTACCATGG GCTGACAAGG 
CTCCCTGTTC ACAGCATTAT AGCAAGCATG GGAACAGTGG CCATGGATCT CCAAGGCTCC 
AGAAGCCCAG AGAGTGTGGA TGGGAATATG CTGAGCCCAC CTGCTGATGG AACTCATACA 
AGGAAACACA GTATAGACGC AGTGCTAGAT GTTGTGGATG GCGACACCAG TACAAATTTC 
GACCTTTTGA GTGACCAATC AGAAGATGAG ACCAGCCAGT CAGACGAGGA GCTGGATTCC 
TCCATCAACC TGGTTAAGGG ATACCCCTCA AACACACCGT ACATTGGCAG CTCACCCACT 
CTGTGCCACT TGCTCCAGCA GAAGAATCCA TATTGTTGCC TGCGTCTTGA CAAGAGCTGC 
CAACATTACC CATATGAAGA TGCAAGGGCA TATGGCTTTA GAAATGCACT GGTGATAGTG 
TCTGCAGAGC ATGCTGGTAA CGGGCTGTAT AACTTTATTG TTCCACTAAG AGCTTACTAC 
AGAGGCAAGA AGGAGCTAAG TCCCATCATC CTGCTTCTGG AGAACCCGCC AGATGTTCAG 
TTCCTGGAAG CCATTTGCTG GTTCCCTCTG GTCTACTACA TGGTTGGATC CATCGATAAT 
CTAGATGACA TGCTGCGCTG TGGGGTCAGT TACGCGGCAC ACATGGTAGT TGTTGGAGAG 
GAAAGCTCTA TGATTGCAGA AGAAGACTAC ATGGCTGACG CGCGCACTAT TGTCAATGTG 
CAGACTTTAT TTAGGCTGTT CCCAGGGTTG AACATCATCA CAGAGCTAAC GCATCCTGCA 
AACATGAGGT TCATGCAGTT CAAAGCCAAG GATAAATACT CCGTTGTACT CTCCAAGCTG 
GAGAAGCTCT CTCCTCCCAC AGAGGGAACG GGAACGGGGC TCGAACCTGG TCTTCATGTT 
CAGACTGCCC TTTGCCGCTG GGAAGGTTTT CAGCGTCAGC ATGTTAGACA CCCTCCTATA 
CCAGCTCCGA GTGACAGAAG AAGACCTGTG GATCCGTTGC TATGGGCGGC TGTATCAGAA 
GCTCTGTTCC ACTACAGGCG ATATTCCCAT TGGGATATAC CGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012808576.1
CDS	2411..5278
Translation	MELEKDVQPLPPRYRFRDLLMGDWQQDDRVHVEFYVNENSFKERLKLFFIKNQRSSLRVRVFNCSLKLLSCLLYIIRVLLDDPRESRGGCWECTKQNYSAQQKFDWTPIIWVNRPLPLWGLQVLVALISFWETMLLTYLGYKGNIWEQLLRIPFILEMLNTLPFFVTIFWPPLRNVFIPVFLNCWLAKHALENMINDLHRAIQRTHSAMFNQVFILICTLICLMFTCICGIQHLERAGNNLTLFDSLYFCIVTFSTVGFGDVTPKIWPSQLLVVIMICVALIVLPIQFEQLVFLWMERQKSGGNYSRYRAQTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQDHYVVLLCPTEMDLPVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKIDDAEACFILSNRFERDRIAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHIKFADHVVCEEEFKYAMLALNCVCPATSTLITLLIHTSRGQDSASSPDQWQNTYSRCSANEVYHIRLSESKFFGEYEGKSFTYSSFHAHKKYGVCLIGVRRDNKTSILLNPGPCHIMSSTDTCFYINISKEENSAFSLKDNEEAKKSGYPKSTYHGLTRLPVHSIIASMGTVAMDLQGSRSPESVDGNMLSPPADGTHTRKHSIDAVLDVVDGDTSTNFDLLSDQSEDETSQSDEELDSSINLVKGYPSNTPYIGSSPTLCHLLQQKNPYCCLRLDKSCQHYPYEDARAYGFRNALVIVSAEHAGNGLYNFIVPLRAYYRGKKELSPIILLLENPPDVQFLEAICWFPLVYYMVGSIDNLDDMLRCGVSYAAHMVVVGEESSMIAEEDYMADARTIVNVQTLFRLFPGLNIITELTHPANMRFMQFKAKDKYSVVLSKLEKLSPPTEGTGTGLEPGLHVQTALCRWEGFQRQHVRHPPIPAPSDRRRPVDPLLWAAVSEALFHYRRYSHWDIPY

Target ORF information:

RefSeq Version	XM_012953122.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis potassium channel, sodium activated subfamily T, member 1 (kcnt1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012953122.2

ORF Insert Sequence:

ATGGAGCTGG AGAAAGATGT GCAACCTTTG CCTCCACGCT ATCGATTTCG GGACTTGTTG 
ATGGGTGACT GGCAACAGGA TGACAGGGTC CATGTGGAAT TTTATGTCAA TGAAAACTCG 
TTTAAGGAAC GACTAAAGTT ATTTTTTATA AAGAATCAGA GATCAAGTTT GAGAGTCAGA 
GTCTTTAACT GTTCCTTAAA GTTACTGAGT TGCCTCCTCT ATATTATAAG GGTCCTTCTG 
GATGATCCGA GAGAGAGCAG GGGCGGATGC TGGGAATGTA CCAAACAGAA TTACTCTGCA 
CAACAGAAAT TTGACTGGAC CCCAATTATT TGGGTAAATA GACCTCTTCC CCTGTGGGGG 
CTTCAGGTAC TCGTGGCGCT AATTAGCTTC TGGGAAACAA TGCTTCTCAC CTACCTTGGC 
TACAAGGGAA ACATATGGGA GCAGCTCCTC CGGATCCCCT TCATTCTAGA GATGCTGAAC 
ACCCTGCCTT TCTTTGTCAC GATTTTCTGG CCTCCGTTGA GGAATGTCTT CATTCCTGTT 
TTCCTCAATT GCTGGTTGGC TAAACATGCG CTGGAGAACA TGATAAATGA CTTGCACCGA 
GCCATTCAGA GGACACACTC GGCCATGTTT AACCAGGTCT TTATTCTGAT ATGCACCCTC 
ATCTGCCTCA TGTTCACTTG CATCTGTGGG ATTCAGCATC TGGAGAGAGC GGGCAATAAC 
CTCACCCTCT TTGACTCCCT GTATTTCTGC ATTGTCACTT TCTCCACGGT GGGCTTCGGA 
GACGTCACCC CAAAGATCTG GCCGTCACAG CTCCTGGTTG TCATTATGAT CTGTGTGGCA 
CTCATTGTGC TGCCCATCCA GTTTGAGCAG CTCGTCTTCC TATGGATGGA AAGGCAGAAA 
TCTGGCGGGA ATTACAGCCG ATACCGAGCG CAGACGGAGA AGCACGTGGT CCTGTGCGTC 
AGTTCTTTGA AGATTGACCT GCTTATGGAT TTCCTCAATG AATTCTATGC TCATCCCCGG 
CTTCAGGACC ATTATGTTGT TCTCCTGTGC CCCACTGAGA TGGACCTGCC TGTGAGGAGA 
GTCCTGCAAA TTCCTCTCTG GTCCCAACGA GTCATCTATC TGCAGGGTTC TGCCCTCAAG 
GACCAGGATC TCATGAGAGC CAAAATTGAT GACGCAGAAG CCTGTTTTAT ACTCAGCAAT 
CGTTTCGAGC GAGACCGCAT CGCAGCTGAC CATCAGACCA TCCTCCGTGC CTGGGCCGTG 
AAGGACTTCG CACCAAACTG TCCCCTCTAC GTTCAGATAC TCAAACCGGA AAACAAGTTT 
CACATCAAAT TTGCAGATCA TGTGGTCTGT GAAGAAGAAT TCAAGTACGC CATGTTGGCT 
CTAAATTGCG TCTGCCCAGC GACTTCCACC CTGATAACTC TGCTCATCCA CACCTCTAGG 
GGGCAAGACA GTGCCAGTTC TCCGGATCAG TGGCAGAACA CCTACAGCCG CTGCTCGGCC 
AATGAAGTTT ATCACATCCG ACTGAGTGAA AGCAAATTCT TTGGAGAGTA TGAAGGGAAA 
AGCTTTACCT ATTCATCCTT CCACGCACAT AAAAAGTACG GGGTCTGTTT GATTGGTGTC 
CGTCGGGATA ACAAAACCAG CATCCTGCTG AATCCAGGGC CTTGCCATAT AATGTCCTCA 
ACAGATACCT GCTTCTACAT AAATATCTCA AAAGAAGAGA ACTCGGCATT CAGTTTGAAA 
GACAACGAAG AAGCCAAGAA ATCCGGGTAC CCAAAGTCTA CCTACCATGG GCTGACAAGG 
CTCCCTGTTC ACAGCATTAT AGCAAGCATG GGAACAGTGG CCATGGATCT CCAAGGCTCC 
AGAAGCCCAG AGAGTGTGGA TGGGAATATG CTGAGCCCAC CTGCTGATGG AACTCATACA 
AGGAAACACA GTATAGACGC AGTGCTAGAT GTTGTGGATG GCGACACCAG TACAAATTTC 
GACCTTTTGA GTGACCAATC AGAAGATGAG ACCAGCCAGT CAGACGAGGA GCTGGATTCC 
TCCATCAACC TGGTTAAGGG ATACCCCTCA AACACACCGT ACATTGGCAG CTCACCCACT 
CTGTGCCACT TGCTCCAGCA GAAGAATCCA TATTGTTGCC TGCGTCTTGA CAAGAGCTGC 
CAACATTACC CATATGAAGA TGCAAGGGCA TATGGCTTTA GAAATGCACT GGTGATAGTG 
TCTGCAGAGC ATGCTGGTAA CGGGCTGTAT AACTTTATTG TTCCACTAAG AGCTTACTAC 
AGAGGCAAGA AGGAGCTAAG TCCCATCATC CTGCTTCTGG AGAACCCGCC AGATGTTCAG 
TTCCTGGAAG CCATTTGCTG GTTCCCTCTG GTCTACTACA TGGTTGGATC CATCGATAAT 
CTAGATGACA TGCTGCGCTG TGGGGTCAGT TACGCGGCAC ACATGGTAGT TGTTGGAGAG 
GAAAGCTCTA TGATTGCAGA AGAAGACTAC ATGGCTGACG CGCGCACTAT TGTCAATGTG 
CAGACTTTAT TTAGGCTGTT CCCAGGGTTG AACATCATCA CAGAGCTAAC GCATCCTGCA 
AACATGAGGT TCATGCAGTT CAAAGCCAAG GATAAATACT CCGTTGTACT CTCCAAGCTG 
GAGAAGCTCT CTCCTCCCAC AGAGGGAACG GGAACGGGGC TCGAACCTGG TCTTCATGTT 
CAGACTGCCC TTTGCCGCTG GGAAGGTTTT CAGCGTCAGC ATGTTAGACA CCCTCCTATA 
CCAGCTCCGA GTGACAGAAG AAGACCTGTG GATCCGTTGC TATGGGCGGC TGTATCAGAA 
GCTCTGTTCC ACTACAGGCG ATATTCCCAT TGGGATATAC CGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kcnt1 ( XM_012953117.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012808571.1 Xenopus tropicalis potassium channel, sodium activated subfamily T, member 2 like (kcnt2l), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa29209
Clone ID Related Accession (Same CDS sequence)	XM_012953117.3 , XM_012953118.2 , XM_012953117.2
Accession Version	XM_012953117.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3480bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	potassium channel subfamily T member 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012953117.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGG AGAAAGATGT GCAACCTTTG CCTCCACGCT ATCGATTTCG GGACTTGTTG 
ATGGGTGACT GGCAACAGGA TGACAGGGTC CATGTGGAAT TTTATGTCAA TGAAAACTCG 
TTTAAGGAAC GACTAAAGTT ATTTTTTATA AAGAATCAGA GATCAAGTTT GAGAGTCAGA 
GTCTTTAACT GTTCCTTAAA GTTACTGAGT TGCCTCCTCT ATATTATAAG GGTCCTTCTG 
GATGATCCGA GAGAGAGCAG GGGCGGATGC TGGGAATGTA CCAAACAGAA TTACTCTGCA 
CAACAGAAAT TTGACTGGAC CCCAATTATT TGGGTAAATA GACCTCTTCC CCTGTGGGGG 
CTTCAGGTAC TCGTGGCGCT AATTAGCTTC TGGGAAACAA TGCTTCTCAC CTACCTTGGC 
TACAAGGGAA ACATATGGGA GCAGCTCCTC CGGATCCCCT TCATTCTAGA GATGCTGAAC 
ACCCTGCCTT TCTTTGTCAC GATTTTCTGG CCTCCGTTGA GGAATGTCTT CATTCCTGTT 
TTCCTCAATT GCTGGTTGGC TAAACATGCG CTGGAGAACA TGATAAATGA CTTGCACCGA 
GCCATTCAGA GGACACACTC GGCCATGTTT AACCAGGTCT TTATTCTGAT ATGCACCCTC 
ATCTGCCTCA TGTTCACTTG CATCTGTGGG ATTCAGCATC TGGAGAGAGC GGGCAATAAC 
CTCACCCTCT TTGACTCCCT GTATTTCTGC ATTGTCACTT TCTCCACGGT GGGCTTCGGA 
GACGTCACCC CAAAGATCTG GCCGTCACAG CTCCTGGTTG TCATTATGAT CTGTGTGGCA 
CTCATTGTGC TGCCCATCCA GTTTGAGCAG CTCGTCTTCC TATGGATGGA AAGGCAGAAA 
TCTGGCGGGA ATTACAGCCG ATACCGAGCG CAGACGGAGA AGCACGTGGT CCTGTGCGTC 
AGTTCTTTGA AGATTGACCT GCTTATGGAT TTCCTCAATG AATTCTATGC TCATCCCCGG 
CTTCAGGACC ATTATGTTGT TCTCCTGTGC CCCACTGAGA TGGACCTGCC TGTGAGGAGA 
GTCCTGCAAA TTCCTCTCTG GTCCCAACGA GTCATCTATC TGCAGGGTTC TGCCCTCAAG 
GACCAGGATC TCATGAGAGC CAAAATTGAT GACGCAGAAG CCTGTTTTAT ACTCAGCAAT 
CGTTTCGAGC GAGACCGCAT CGCAGCTGAC CATCAGACCA TCCTCCGTGC CTGGGCCGTG 
AAGGACTTCG CACCAAACTG TCCCCTCTAC GTTCAGATAC TCAAACCGGA AAACAAGTTT 
CACATCAAAT TTGCAGATCA TGTGGTCTGT GAAGAAGAAT TCAAGTACGC CATGTTGGCT 
CTAAATTGCG TCTGCCCAGC GACTTCCACC CTGATAACTC TGCTCATCCA CACCTCTAGG 
GGGCAAGACA GTGCCAGTTC TCCGGATCAG TGGCAGAACA CCTACAGCCG CTGCTCGGCC 
AATGAAGTTT ATCACATCCG ACTGAGTGAA AGCAAATTCT TTGGAGAGTA TGAAGGGAAA 
AGCTTTACCT ATTCATCCTT CCACGCACAT AAAAAGTACG GGGTCTGTTT GATTGGTGTC 
CGTCGGGATA ACAAAACCAG CATCCTGCTG AATCCAGGGC CTTGCCATAT AATGTCCTCA 
ACAGATACCT GCTTCTACAT AAATATCTCA AAAGAAGAGA ACTCGGCATT CAGTTTGAAA 
GACAACGAAG AAGCCAAGAA ATCCGGGTAC CCAAAGTCTA CCTACCATGG GCTGACAAGG 
CTCCCTGTTC ACAGCATTAT AGCAAGCATG GGAACAGTGG CCATGGATCT CCAAGGCTCC 
AGAAGCCCAG AGAGTGTGGA TGGGAATATG CTGAGCCCAC CTGCTGATGG AACTCATACA 
AGGAAACACA GTATAGACGC AGTGCTAGAT GTTGTGGATG GCGACACCAG TACAAATTTC 
GACCTTTTGA GTGACCAATC AGAAGATGAG ACCAGCCAGT CAGACGAGGA GCTGGATTCC 
TCCATCAACC TGGTTAAGGG ATACCCCTCA AACACACCGT ACATTGGCAG CTCACCCACT 
CTGTGCCACT TGCTCCAGCA GAAGAATCCA TATTGTTGCC TGCGTCTTGA CAAGAGCTGC 
CAACATTACC CATATGAAGA TGCAAGGGCA TATGGCTTTA GAAATGCACT GGTGATAGTG 
TCTGCAGAGC ATGCTGGTAA CGGGCTGTAT AACTTTATTG TTCCACTAAG AGCTTACTAC 
AGAGGCAAGA AGGAGCTAAG TCCCATCATC CTGCTTCTGG AGAACCCGCC AGATGTTCAG 
TTCCTGGAAG CCATTTGCTG GTTCCCTCTG GTCTACTACA TGGTTGGATC CATCGATAAT 
CTAGATGACA TGCTGCGCTG TGGGGTCAGT TACGCGGCAC ACATGGTAGT TGTTGGAGAG 
GAAAGCTCTA TGATTGCAGA AGAAGACTAC ATGGCTGACG CGCGCACTAT TGTCAATGTG 
CAGACTTTAT TTAGGCTGTT CCCAGGGTTG AACATCATCA CAGAGCTAAC GCATCCTGCA 
AACATGAGGT TCATGCAGTT CAAAGCCAAG GATAAATACT CCGTTGTACT CTCCAAGCTG 
GAGAAGAGGG AACGGGAACG GGGCTCGAAC CTGGTCTTCA TGTTCAGACT GCCCTTTGCC 
GCTGGGAAGG TTTTCAGCGT CAGCATGTTA GACACCCTCC TATACCAGTC ATTTGTTAAG 
GATTACATGA TCTCCATCAC ACGACTGCTG CTCGGCCTGG ATTCCATGCC TGGTTCTGGA 
TTCCTCTGTG CCCTCCGAGT GACAGAAGAA GACCTGTGGA TCCGTTGCTA TGGGCGGCTG 
TATCAGAAGC TCTGTTCCAC TACAGGCGAT ATTCCCATTG GGATATACCG TACTGAGACA 
CACTTGCTGC CTGCCTCTGA GTCTCAGGTA TCAATCAGTG TTGAAGATTA TGAAGATACA 
AGAGAGACTC GATGGGGAGA CCCTAGCCAG GGGCGACCCC TTCACCGGAA CTCAACCTCC 
AGTGACCAAT CAGAGCACCC CTTACTGCGC AGAAAGAGCA TGCAGTGGGC CAGAAGGCTA 
AGCCGCAAGG GAGCAAGGCA GCCTGGGAAA TCGGCTTCGG AACGAATCAG CCAGCAGCGA 
ATGAGTCTGT ACCGGCGGTC GGAGCGGCAG GAACTCAATG CACTGGTCAA GACCAGGATG 
AAGCATTTGG GGCTCTCCAC TGCTGAGTAC AGTATGAAAC AGATGGACAT TATGGACACT 
GACAACACTT TGTCCTATAT CCTAATAAAC CCATCTCCAG ATACCAAACT GGAACTTAAT 
GATGTTGTTT ACCTCATCCG ACCAGACCCT CTTACTCTGG TGTCTATGAA GTCCCGTAAA 
GCTAGCGTCA CACGCTCGGA AGGCACGGTA TACGACATGA AGGACAGCAC CCATCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012808571.1
CDS	1475..4954
Translation	MELEKDVQPLPPRYRFRDLLMGDWQQDDRVHVEFYVNENSFKERLKLFFIKNQRSSLRVRVFNCSLKLLSCLLYIIRVLLDDPRESRGGCWECTKQNYSAQQKFDWTPIIWVNRPLPLWGLQVLVALISFWETMLLTYLGYKGNIWEQLLRIPFILEMLNTLPFFVTIFWPPLRNVFIPVFLNCWLAKHALENMINDLHRAIQRTHSAMFNQVFILICTLICLMFTCICGIQHLERAGNNLTLFDSLYFCIVTFSTVGFGDVTPKIWPSQLLVVIMICVALIVLPIQFEQLVFLWMERQKSGGNYSRYRAQTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQDHYVVLLCPTEMDLPVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKIDDAEACFILSNRFERDRIAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHIKFADHVVCEEEFKYAMLALNCVCPATSTLITLLIHTSRGQDSASSPDQWQNTYSRCSANEVYHIRLSESKFFGEYEGKSFTYSSFHAHKKYGVCLIGVRRDNKTSILLNPGPCHIMSSTDTCFYINISKEENSAFSLKDNEEAKKSGYPKSTYHGLTRLPVHSIIASMGTVAMDLQGSRSPESVDGNMLSPPADGTHTRKHSIDAVLDVVDGDTSTNFDLLSDQSEDETSQSDEELDSSINLVKGYPSNTPYIGSSPTLCHLLQQKNPYCCLRLDKSCQHYPYEDARAYGFRNALVIVSAEHAGNGLYNFIVPLRAYYRGKKELSPIILLLENPPDVQFLEAICWFPLVYYMVGSIDNLDDMLRCGVSYAAHMVVVGEESSMIAEEDYMADARTIVNVQTLFRLFPGLNIITELTHPANMRFMQFKAKDKYSVVLSKLEKRERERGSNLVFMFRLPFAAGKVFSVSMLDTLLYQSFVKDYMISITRLLLGLDSMPGSGFLCALRVTEEDLWIRCYGRLYQKLCSTTGDIPIGIYRTETHLLPASESQVSISVEDYEDTRETRWGDPSQGRPLHRNSTSSDQSEHPLLRRKSMQWARRLSRKGARQPGKSASERISQQRMSLYRRSERQELNALVKTRMKHLGLSTAEYSMKQMDIMDTDNTLSYILINPSPDTKLELNDVVYLIRPDPLTLVSMKSRKASVTRSEGTVYDMKDSTHL

Target ORF information:

RefSeq Version	XM_012953117.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis potassium channel, sodium activated subfamily T, member 2 like (kcnt2l), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012953117.3

ORF Insert Sequence:

ATGGAGCTGG AGAAAGATGT GCAACCTTTG CCTCCACGCT ATCGATTTCG GGACTTGTTG 
ATGGGTGACT GGCAACAGGA TGACAGGGTC CATGTGGAAT TTTATGTCAA TGAAAACTCG 
TTTAAGGAAC GACTAAAGTT ATTTTTTATA AAGAATCAGA GATCAAGTTT GAGAGTCAGA 
GTCTTTAACT GTTCCTTAAA GTTACTGAGT TGCCTCCTCT ATATTATAAG GGTCCTTCTG 
GATGATCCGA GAGAGAGCAG GGGCGGATGC TGGGAATGTA CCAAACAGAA TTACTCTGCA 
CAACAGAAAT TTGACTGGAC CCCAATTATT TGGGTAAATA GACCTCTTCC CCTGTGGGGG 
CTTCAGGTAC TCGTGGCGCT AATTAGCTTC TGGGAAACAA TGCTTCTCAC CTACCTTGGC 
TACAAGGGAA ACATATGGGA GCAGCTCCTC CGGATCCCCT TCATTCTAGA GATGCTGAAC 
ACCCTGCCTT TCTTTGTCAC GATTTTCTGG CCTCCGTTGA GGAATGTCTT CATTCCTGTT 
TTCCTCAATT GCTGGTTGGC TAAACATGCG CTGGAGAACA TGATAAATGA CTTGCACCGA 
GCCATTCAGA GGACACACTC GGCCATGTTT AACCAGGTCT TTATTCTGAT ATGCACCCTC 
ATCTGCCTCA TGTTCACTTG CATCTGTGGG ATTCAGCATC TGGAGAGAGC GGGCAATAAC 
CTCACCCTCT TTGACTCCCT GTATTTCTGC ATTGTCACTT TCTCCACGGT GGGCTTCGGA 
GACGTCACCC CAAAGATCTG GCCGTCACAG CTCCTGGTTG TCATTATGAT CTGTGTGGCA 
CTCATTGTGC TGCCCATCCA GTTTGAGCAG CTCGTCTTCC TATGGATGGA AAGGCAGAAA 
TCTGGCGGGA ATTACAGCCG ATACCGAGCG CAGACGGAGA AGCACGTGGT CCTGTGCGTC 
AGTTCTTTGA AGATTGACCT GCTTATGGAT TTCCTCAATG AATTCTATGC TCATCCCCGG 
CTTCAGGACC ATTATGTTGT TCTCCTGTGC CCCACTGAGA TGGACCTGCC TGTGAGGAGA 
GTCCTGCAAA TTCCTCTCTG GTCCCAACGA GTCATCTATC TGCAGGGTTC TGCCCTCAAG 
GACCAGGATC TCATGAGAGC CAAAATTGAT GACGCAGAAG CCTGTTTTAT ACTCAGCAAT 
CGTTTCGAGC GAGACCGCAT CGCAGCTGAC CATCAGACCA TCCTCCGTGC CTGGGCCGTG 
AAGGACTTCG CACCAAACTG TCCCCTCTAC GTTCAGATAC TCAAACCGGA AAACAAGTTT 
CACATCAAAT TTGCAGATCA TGTGGTCTGT GAAGAAGAAT TCAAGTACGC CATGTTGGCT 
CTAAATTGCG TCTGCCCAGC GACTTCCACC CTGATAACTC TGCTCATCCA CACCTCTAGG 
GGGCAAGACA GTGCCAGTTC TCCGGATCAG TGGCAGAACA CCTACAGCCG CTGCTCGGCC 
AATGAAGTTT ATCACATCCG ACTGAGTGAA AGCAAATTCT TTGGAGAGTA TGAAGGGAAA 
AGCTTTACCT ATTCATCCTT CCACGCACAT AAAAAGTACG GGGTCTGTTT GATTGGTGTC 
CGTCGGGATA ACAAAACCAG CATCCTGCTG AATCCAGGGC CTTGCCATAT AATGTCCTCA 
ACAGATACCT GCTTCTACAT AAATATCTCA AAAGAAGAGA ACTCGGCATT CAGTTTGAAA 
GACAACGAAG AAGCCAAGAA ATCCGGGTAC CCAAAGTCTA CCTACCATGG GCTGACAAGG 
CTCCCTGTTC ACAGCATTAT AGCAAGCATG GGAACAGTGG CCATGGATCT CCAAGGCTCC 
AGAAGCCCAG AGAGTGTGGA TGGGAATATG CTGAGCCCAC CTGCTGATGG AACTCATACA 
AGGAAACACA GTATAGACGC AGTGCTAGAT GTTGTGGATG GCGACACCAG TACAAATTTC 
GACCTTTTGA GTGACCAATC AGAAGATGAG ACCAGCCAGT CAGACGAGGA GCTGGATTCC 
TCCATCAACC TGGTTAAGGG ATACCCCTCA AACACACCGT ACATTGGCAG CTCACCCACT 
CTGTGCCACT TGCTCCAGCA GAAGAATCCA TATTGTTGCC TGCGTCTTGA CAAGAGCTGC 
CAACATTACC CATATGAAGA TGCAAGGGCA TATGGCTTTA GAAATGCACT GGTGATAGTG 
TCTGCAGAGC ATGCTGGTAA CGGGCTGTAT AACTTTATTG TTCCACTAAG AGCTTACTAC 
AGAGGCAAGA AGGAGCTAAG TCCCATCATC CTGCTTCTGG AGAACCCGCC AGATGTTCAG 
TTCCTGGAAG CCATTTGCTG GTTCCCTCTG GTCTACTACA TGGTTGGATC CATCGATAAT 
CTAGATGACA TGCTGCGCTG TGGGGTCAGT TACGCGGCAC ACATGGTAGT TGTTGGAGAG 
GAAAGCTCTA TGATTGCAGA AGAAGACTAC ATGGCTGACG CGCGCACTAT TGTCAATGTG 
CAGACTTTAT TTAGGCTGTT CCCAGGGTTG AACATCATCA CAGAGCTAAC GCATCCTGCA 
AACATGAGGT TCATGCAGTT CAAAGCCAAG GATAAATACT CCGTTGTACT CTCCAAGCTG 
GAGAAGAGGG AACGGGAACG GGGCTCGAAC CTGGTCTTCA TGTTCAGACT GCCCTTTGCC 
GCTGGGAAGG TTTTCAGCGT CAGCATGTTA GACACCCTCC TATACCAGTC ATTTGTTAAG 
GATTACATGA TCTCCATCAC ACGACTGCTG CTCGGCCTGG ATTCCATGCC TGGTTCTGGA 
TTCCTCTGTG CCCTCCGAGT GACAGAAGAA GACCTGTGGA TCCGTTGCTA TGGGCGGCTG 
TATCAGAAGC TCTGTTCCAC TACAGGCGAT ATTCCCATTG GGATATACCG TACTGAGACA 
CACTTGCTGC CTGCCTCTGA GTCTCAGGTA TCAATCAGTG TTGAAGATTA TGAAGATACA 
AGAGAGACTC GATGGGGAGA CCCTAGCCAG GGGCGACCCC TTCACCGGAA CTCAACCTCC 
AGTGACCAAT CAGAGCACCC CTTACTGCGC AGAAAGAGCA TGCAGTGGGC CAGAAGGCTA 
AGCCGCAAGG GAGCAAGGCA GCCTGGGAAA TCGGCTTCGG AACGAATCAG CCAGCAGCGA 
ATGAGTCTGT ACCGGCGGTC GGAGCGGCAG GAACTCAATG CACTGGTCAA GACCAGGATG 
AAGCATTTGG GGCTCTCCAC TGCTGAGTAC AGTATGAAAC AGATGGACAT TATGGACACT 
GACAACACTT TGTCCTATAT CCTAATAAAC CCATCTCCAG ATACCAAACT GGAACTTAAT 
GATGTTGTTT ACCTCATCCG ACCAGACCCT CTTACTCTGG TGTCTATGAA GTCCCGTAAA 
GCTAGCGTCA CACGCTCGGA AGGCACGGTA TACGACATGA AGGACAGCAC CCATCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kcnt1 ( XM_012953118.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012808572.1 Xenopus tropicalis potassium channel, sodium activated subfamily T, member 1 (kcnt1), transcript variant X2, mRNA.

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CloneID	OXa29209
Clone ID Related Accession (Same CDS sequence)	XM_012953117.3 , XM_012953118.2 , XM_012953117.2
Accession Version	XM_012953118.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3480bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	potassium channel subfamily T member 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012953118.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGG AGAAAGATGT GCAACCTTTG CCTCCACGCT ATCGATTTCG GGACTTGTTG 
ATGGGTGACT GGCAACAGGA TGACAGGGTC CATGTGGAAT TTTATGTCAA TGAAAACTCG 
TTTAAGGAAC GACTAAAGTT ATTTTTTATA AAGAATCAGA GATCAAGTTT GAGAGTCAGA 
GTCTTTAACT GTTCCTTAAA GTTACTGAGT TGCCTCCTCT ATATTATAAG GGTCCTTCTG 
GATGATCCGA GAGAGAGCAG GGGCGGATGC TGGGAATGTA CCAAACAGAA TTACTCTGCA 
CAACAGAAAT TTGACTGGAC CCCAATTATT TGGGTAAATA GACCTCTTCC CCTGTGGGGG 
CTTCAGGTAC TCGTGGCGCT AATTAGCTTC TGGGAAACAA TGCTTCTCAC CTACCTTGGC 
TACAAGGGAA ACATATGGGA GCAGCTCCTC CGGATCCCCT TCATTCTAGA GATGCTGAAC 
ACCCTGCCTT TCTTTGTCAC GATTTTCTGG CCTCCGTTGA GGAATGTCTT CATTCCTGTT 
TTCCTCAATT GCTGGTTGGC TAAACATGCG CTGGAGAACA TGATAAATGA CTTGCACCGA 
GCCATTCAGA GGACACACTC GGCCATGTTT AACCAGGTCT TTATTCTGAT ATGCACCCTC 
ATCTGCCTCA TGTTCACTTG CATCTGTGGG ATTCAGCATC TGGAGAGAGC GGGCAATAAC 
CTCACCCTCT TTGACTCCCT GTATTTCTGC ATTGTCACTT TCTCCACGGT GGGCTTCGGA 
GACGTCACCC CAAAGATCTG GCCGTCACAG CTCCTGGTTG TCATTATGAT CTGTGTGGCA 
CTCATTGTGC TGCCCATCCA GTTTGAGCAG CTCGTCTTCC TATGGATGGA AAGGCAGAAA 
TCTGGCGGGA ATTACAGCCG ATACCGAGCG CAGACGGAGA AGCACGTGGT CCTGTGCGTC 
AGTTCTTTGA AGATTGACCT GCTTATGGAT TTCCTCAATG AATTCTATGC TCATCCCCGG 
CTTCAGGACC ATTATGTTGT TCTCCTGTGC CCCACTGAGA TGGACCTGCC TGTGAGGAGA 
GTCCTGCAAA TTCCTCTCTG GTCCCAACGA GTCATCTATC TGCAGGGTTC TGCCCTCAAG 
GACCAGGATC TCATGAGAGC CAAAATTGAT GACGCAGAAG CCTGTTTTAT ACTCAGCAAT 
CGTTTCGAGC GAGACCGCAT CGCAGCTGAC CATCAGACCA TCCTCCGTGC CTGGGCCGTG 
AAGGACTTCG CACCAAACTG TCCCCTCTAC GTTCAGATAC TCAAACCGGA AAACAAGTTT 
CACATCAAAT TTGCAGATCA TGTGGTCTGT GAAGAAGAAT TCAAGTACGC CATGTTGGCT 
CTAAATTGCG TCTGCCCAGC GACTTCCACC CTGATAACTC TGCTCATCCA CACCTCTAGG 
GGGCAAGACA GTGCCAGTTC TCCGGATCAG TGGCAGAACA CCTACAGCCG CTGCTCGGCC 
AATGAAGTTT ATCACATCCG ACTGAGTGAA AGCAAATTCT TTGGAGAGTA TGAAGGGAAA 
AGCTTTACCT ATTCATCCTT CCACGCACAT AAAAAGTACG GGGTCTGTTT GATTGGTGTC 
CGTCGGGATA ACAAAACCAG CATCCTGCTG AATCCAGGGC CTTGCCATAT AATGTCCTCA 
ACAGATACCT GCTTCTACAT AAATATCTCA AAAGAAGAGA ACTCGGCATT CAGTTTGAAA 
GACAACGAAG AAGCCAAGAA ATCCGGGTAC CCAAAGTCTA CCTACCATGG GCTGACAAGG 
CTCCCTGTTC ACAGCATTAT AGCAAGCATG GGAACAGTGG CCATGGATCT CCAAGGCTCC 
AGAAGCCCAG AGAGTGTGGA TGGGAATATG CTGAGCCCAC CTGCTGATGG AACTCATACA 
AGGAAACACA GTATAGACGC AGTGCTAGAT GTTGTGGATG GCGACACCAG TACAAATTTC 
GACCTTTTGA GTGACCAATC AGAAGATGAG ACCAGCCAGT CAGACGAGGA GCTGGATTCC 
TCCATCAACC TGGTTAAGGG ATACCCCTCA AACACACCGT ACATTGGCAG CTCACCCACT 
CTGTGCCACT TGCTCCAGCA GAAGAATCCA TATTGTTGCC TGCGTCTTGA CAAGAGCTGC 
CAACATTACC CATATGAAGA TGCAAGGGCA TATGGCTTTA GAAATGCACT GGTGATAGTG 
TCTGCAGAGC ATGCTGGTAA CGGGCTGTAT AACTTTATTG TTCCACTAAG AGCTTACTAC 
AGAGGCAAGA AGGAGCTAAG TCCCATCATC CTGCTTCTGG AGAACCCGCC AGATGTTCAG 
TTCCTGGAAG CCATTTGCTG GTTCCCTCTG GTCTACTACA TGGTTGGATC CATCGATAAT 
CTAGATGACA TGCTGCGCTG TGGGGTCAGT TACGCGGCAC ACATGGTAGT TGTTGGAGAG 
GAAAGCTCTA TGATTGCAGA AGAAGACTAC ATGGCTGACG CGCGCACTAT TGTCAATGTG 
CAGACTTTAT TTAGGCTGTT CCCAGGGTTG AACATCATCA CAGAGCTAAC GCATCCTGCA 
AACATGAGGT TCATGCAGTT CAAAGCCAAG GATAAATACT CCGTTGTACT CTCCAAGCTG 
GAGAAGAGGG AACGGGAACG GGGCTCGAAC CTGGTCTTCA TGTTCAGACT GCCCTTTGCC 
GCTGGGAAGG TTTTCAGCGT CAGCATGTTA GACACCCTCC TATACCAGTC ATTTGTTAAG 
GATTACATGA TCTCCATCAC ACGACTGCTG CTCGGCCTGG ATTCCATGCC TGGTTCTGGA 
TTCCTCTGTG CCCTCCGAGT GACAGAAGAA GACCTGTGGA TCCGTTGCTA TGGGCGGCTG 
TATCAGAAGC TCTGTTCCAC TACAGGCGAT ATTCCCATTG GGATATACCG TACTGAGACA 
CACTTGCTGC CTGCCTCTGA GTCTCAGGTA TCAATCAGTG TTGAAGATTA TGAAGATACA 
AGAGAGACTC GATGGGGAGA CCCTAGCCAG GGGCGACCCC TTCACCGGAA CTCAACCTCC 
AGTGACCAAT CAGAGCACCC CTTACTGCGC AGAAAGAGCA TGCAGTGGGC CAGAAGGCTA 
AGCCGCAAGG GAGCAAGGCA GCCTGGGAAA TCGGCTTCGG AACGAATCAG CCAGCAGCGA 
ATGAGTCTGT ACCGGCGGTC GGAGCGGCAG GAACTCAATG CACTGGTCAA GACCAGGATG 
AAGCATTTGG GGCTCTCCAC TGCTGAGTAC AGTATGAAAC AGATGGACAT TATGGACACT 
GACAACACTT TGTCCTATAT CCTAATAAAC CCATCTCCAG ATACCAAACT GGAACTTAAT 
GATGTTGTTT ACCTCATCCG ACCAGACCCT CTTACTCTGG TGTCTATGAA GTCCCGTAAA 
GCTAGCGTCA CACGCTCGGA AGGCACGGTA TACGACATGA AGGACAGCAC CCATCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012808572.1
CDS	2398..5877
Translation	MELEKDVQPLPPRYRFRDLLMGDWQQDDRVHVEFYVNENSFKERLKLFFIKNQRSSLRVRVFNCSLKLLSCLLYIIRVLLDDPRESRGGCWECTKQNYSAQQKFDWTPIIWVNRPLPLWGLQVLVALISFWETMLLTYLGYKGNIWEQLLRIPFILEMLNTLPFFVTIFWPPLRNVFIPVFLNCWLAKHALENMINDLHRAIQRTHSAMFNQVFILICTLICLMFTCICGIQHLERAGNNLTLFDSLYFCIVTFSTVGFGDVTPKIWPSQLLVVIMICVALIVLPIQFEQLVFLWMERQKSGGNYSRYRAQTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQDHYVVLLCPTEMDLPVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKIDDAEACFILSNRFERDRIAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHIKFADHVVCEEEFKYAMLALNCVCPATSTLITLLIHTSRGQDSASSPDQWQNTYSRCSANEVYHIRLSESKFFGEYEGKSFTYSSFHAHKKYGVCLIGVRRDNKTSILLNPGPCHIMSSTDTCFYINISKEENSAFSLKDNEEAKKSGYPKSTYHGLTRLPVHSIIASMGTVAMDLQGSRSPESVDGNMLSPPADGTHTRKHSIDAVLDVVDGDTSTNFDLLSDQSEDETSQSDEELDSSINLVKGYPSNTPYIGSSPTLCHLLQQKNPYCCLRLDKSCQHYPYEDARAYGFRNALVIVSAEHAGNGLYNFIVPLRAYYRGKKELSPIILLLENPPDVQFLEAICWFPLVYYMVGSIDNLDDMLRCGVSYAAHMVVVGEESSMIAEEDYMADARTIVNVQTLFRLFPGLNIITELTHPANMRFMQFKAKDKYSVVLSKLEKRERERGSNLVFMFRLPFAAGKVFSVSMLDTLLYQSFVKDYMISITRLLLGLDSMPGSGFLCALRVTEEDLWIRCYGRLYQKLCSTTGDIPIGIYRTETHLLPASESQVSISVEDYEDTRETRWGDPSQGRPLHRNSTSSDQSEHPLLRRKSMQWARRLSRKGARQPGKSASERISQQRMSLYRRSERQELNALVKTRMKHLGLSTAEYSMKQMDIMDTDNTLSYILINPSPDTKLELNDVVYLIRPDPLTLVSMKSRKASVTRSEGTVYDMKDSTHL

Target ORF information:

RefSeq Version	XM_012953118.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis potassium channel, sodium activated subfamily T, member 1 (kcnt1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012953118.2

ORF Insert Sequence:

ATGGAGCTGG AGAAAGATGT GCAACCTTTG CCTCCACGCT ATCGATTTCG GGACTTGTTG 
ATGGGTGACT GGCAACAGGA TGACAGGGTC CATGTGGAAT TTTATGTCAA TGAAAACTCG 
TTTAAGGAAC GACTAAAGTT ATTTTTTATA AAGAATCAGA GATCAAGTTT GAGAGTCAGA 
GTCTTTAACT GTTCCTTAAA GTTACTGAGT TGCCTCCTCT ATATTATAAG GGTCCTTCTG 
GATGATCCGA GAGAGAGCAG GGGCGGATGC TGGGAATGTA CCAAACAGAA TTACTCTGCA 
CAACAGAAAT TTGACTGGAC CCCAATTATT TGGGTAAATA GACCTCTTCC CCTGTGGGGG 
CTTCAGGTAC TCGTGGCGCT AATTAGCTTC TGGGAAACAA TGCTTCTCAC CTACCTTGGC 
TACAAGGGAA ACATATGGGA GCAGCTCCTC CGGATCCCCT TCATTCTAGA GATGCTGAAC 
ACCCTGCCTT TCTTTGTCAC GATTTTCTGG CCTCCGTTGA GGAATGTCTT CATTCCTGTT 
TTCCTCAATT GCTGGTTGGC TAAACATGCG CTGGAGAACA TGATAAATGA CTTGCACCGA 
GCCATTCAGA GGACACACTC GGCCATGTTT AACCAGGTCT TTATTCTGAT ATGCACCCTC 
ATCTGCCTCA TGTTCACTTG CATCTGTGGG ATTCAGCATC TGGAGAGAGC GGGCAATAAC 
CTCACCCTCT TTGACTCCCT GTATTTCTGC ATTGTCACTT TCTCCACGGT GGGCTTCGGA 
GACGTCACCC CAAAGATCTG GCCGTCACAG CTCCTGGTTG TCATTATGAT CTGTGTGGCA 
CTCATTGTGC TGCCCATCCA GTTTGAGCAG CTCGTCTTCC TATGGATGGA AAGGCAGAAA 
TCTGGCGGGA ATTACAGCCG ATACCGAGCG CAGACGGAGA AGCACGTGGT CCTGTGCGTC 
AGTTCTTTGA AGATTGACCT GCTTATGGAT TTCCTCAATG AATTCTATGC TCATCCCCGG 
CTTCAGGACC ATTATGTTGT TCTCCTGTGC CCCACTGAGA TGGACCTGCC TGTGAGGAGA 
GTCCTGCAAA TTCCTCTCTG GTCCCAACGA GTCATCTATC TGCAGGGTTC TGCCCTCAAG 
GACCAGGATC TCATGAGAGC CAAAATTGAT GACGCAGAAG CCTGTTTTAT ACTCAGCAAT 
CGTTTCGAGC GAGACCGCAT CGCAGCTGAC CATCAGACCA TCCTCCGTGC CTGGGCCGTG 
AAGGACTTCG CACCAAACTG TCCCCTCTAC GTTCAGATAC TCAAACCGGA AAACAAGTTT 
CACATCAAAT TTGCAGATCA TGTGGTCTGT GAAGAAGAAT TCAAGTACGC CATGTTGGCT 
CTAAATTGCG TCTGCCCAGC GACTTCCACC CTGATAACTC TGCTCATCCA CACCTCTAGG 
GGGCAAGACA GTGCCAGTTC TCCGGATCAG TGGCAGAACA CCTACAGCCG CTGCTCGGCC 
AATGAAGTTT ATCACATCCG ACTGAGTGAA AGCAAATTCT TTGGAGAGTA TGAAGGGAAA 
AGCTTTACCT ATTCATCCTT CCACGCACAT AAAAAGTACG GGGTCTGTTT GATTGGTGTC 
CGTCGGGATA ACAAAACCAG CATCCTGCTG AATCCAGGGC CTTGCCATAT AATGTCCTCA 
ACAGATACCT GCTTCTACAT AAATATCTCA AAAGAAGAGA ACTCGGCATT CAGTTTGAAA 
GACAACGAAG AAGCCAAGAA ATCCGGGTAC CCAAAGTCTA CCTACCATGG GCTGACAAGG 
CTCCCTGTTC ACAGCATTAT AGCAAGCATG GGAACAGTGG CCATGGATCT CCAAGGCTCC 
AGAAGCCCAG AGAGTGTGGA TGGGAATATG CTGAGCCCAC CTGCTGATGG AACTCATACA 
AGGAAACACA GTATAGACGC AGTGCTAGAT GTTGTGGATG GCGACACCAG TACAAATTTC 
GACCTTTTGA GTGACCAATC AGAAGATGAG ACCAGCCAGT CAGACGAGGA GCTGGATTCC 
TCCATCAACC TGGTTAAGGG ATACCCCTCA AACACACCGT ACATTGGCAG CTCACCCACT 
CTGTGCCACT TGCTCCAGCA GAAGAATCCA TATTGTTGCC TGCGTCTTGA CAAGAGCTGC 
CAACATTACC CATATGAAGA TGCAAGGGCA TATGGCTTTA GAAATGCACT GGTGATAGTG 
TCTGCAGAGC ATGCTGGTAA CGGGCTGTAT AACTTTATTG TTCCACTAAG AGCTTACTAC 
AGAGGCAAGA AGGAGCTAAG TCCCATCATC CTGCTTCTGG AGAACCCGCC AGATGTTCAG 
TTCCTGGAAG CCATTTGCTG GTTCCCTCTG GTCTACTACA TGGTTGGATC CATCGATAAT 
CTAGATGACA TGCTGCGCTG TGGGGTCAGT TACGCGGCAC ACATGGTAGT TGTTGGAGAG 
GAAAGCTCTA TGATTGCAGA AGAAGACTAC ATGGCTGACG CGCGCACTAT TGTCAATGTG 
CAGACTTTAT TTAGGCTGTT CCCAGGGTTG AACATCATCA CAGAGCTAAC GCATCCTGCA 
AACATGAGGT TCATGCAGTT CAAAGCCAAG GATAAATACT CCGTTGTACT CTCCAAGCTG 
GAGAAGAGGG AACGGGAACG GGGCTCGAAC CTGGTCTTCA TGTTCAGACT GCCCTTTGCC 
GCTGGGAAGG TTTTCAGCGT CAGCATGTTA GACACCCTCC TATACCAGTC ATTTGTTAAG 
GATTACATGA TCTCCATCAC ACGACTGCTG CTCGGCCTGG ATTCCATGCC TGGTTCTGGA 
TTCCTCTGTG CCCTCCGAGT GACAGAAGAA GACCTGTGGA TCCGTTGCTA TGGGCGGCTG 
TATCAGAAGC TCTGTTCCAC TACAGGCGAT ATTCCCATTG GGATATACCG TACTGAGACA 
CACTTGCTGC CTGCCTCTGA GTCTCAGGTA TCAATCAGTG TTGAAGATTA TGAAGATACA 
AGAGAGACTC GATGGGGAGA CCCTAGCCAG GGGCGACCCC TTCACCGGAA CTCAACCTCC 
AGTGACCAAT CAGAGCACCC CTTACTGCGC AGAAAGAGCA TGCAGTGGGC CAGAAGGCTA 
AGCCGCAAGG GAGCAAGGCA GCCTGGGAAA TCGGCTTCGG AACGAATCAG CCAGCAGCGA 
ATGAGTCTGT ACCGGCGGTC GGAGCGGCAG GAACTCAATG CACTGGTCAA GACCAGGATG 
AAGCATTTGG GGCTCTCCAC TGCTGAGTAC AGTATGAAAC AGATGGACAT TATGGACACT 
GACAACACTT TGTCCTATAT CCTAATAAAC CCATCTCCAG ATACCAAACT GGAACTTAAT 
GATGTTGTTT ACCTCATCCG ACCAGACCCT CTTACTCTGG TGTCTATGAA GTCCCGTAAA 
GCTAGCGTCA CACGCTCGGA AGGCACGGTA TACGACATGA AGGACAGCAC CCATCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kcnt1 ( XM_012953117.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012808571.1 Xenopus tropicalis potassium channel, sodium activated subfamily T, member 1 (kcnt1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa29209
Clone ID Related Accession (Same CDS sequence)	XM_012953117.3 , XM_012953118.2 , XM_012953117.2
Accession Version	XM_012953117.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3480bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	potassium channel subfamily T member 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012953117.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGG AGAAAGATGT GCAACCTTTG CCTCCACGCT ATCGATTTCG GGACTTGTTG 
ATGGGTGACT GGCAACAGGA TGACAGGGTC CATGTGGAAT TTTATGTCAA TGAAAACTCG 
TTTAAGGAAC GACTAAAGTT ATTTTTTATA AAGAATCAGA GATCAAGTTT GAGAGTCAGA 
GTCTTTAACT GTTCCTTAAA GTTACTGAGT TGCCTCCTCT ATATTATAAG GGTCCTTCTG 
GATGATCCGA GAGAGAGCAG GGGCGGATGC TGGGAATGTA CCAAACAGAA TTACTCTGCA 
CAACAGAAAT TTGACTGGAC CCCAATTATT TGGGTAAATA GACCTCTTCC CCTGTGGGGG 
CTTCAGGTAC TCGTGGCGCT AATTAGCTTC TGGGAAACAA TGCTTCTCAC CTACCTTGGC 
TACAAGGGAA ACATATGGGA GCAGCTCCTC CGGATCCCCT TCATTCTAGA GATGCTGAAC 
ACCCTGCCTT TCTTTGTCAC GATTTTCTGG CCTCCGTTGA GGAATGTCTT CATTCCTGTT 
TTCCTCAATT GCTGGTTGGC TAAACATGCG CTGGAGAACA TGATAAATGA CTTGCACCGA 
GCCATTCAGA GGACACACTC GGCCATGTTT AACCAGGTCT TTATTCTGAT ATGCACCCTC 
ATCTGCCTCA TGTTCACTTG CATCTGTGGG ATTCAGCATC TGGAGAGAGC GGGCAATAAC 
CTCACCCTCT TTGACTCCCT GTATTTCTGC ATTGTCACTT TCTCCACGGT GGGCTTCGGA 
GACGTCACCC CAAAGATCTG GCCGTCACAG CTCCTGGTTG TCATTATGAT CTGTGTGGCA 
CTCATTGTGC TGCCCATCCA GTTTGAGCAG CTCGTCTTCC TATGGATGGA AAGGCAGAAA 
TCTGGCGGGA ATTACAGCCG ATACCGAGCG CAGACGGAGA AGCACGTGGT CCTGTGCGTC 
AGTTCTTTGA AGATTGACCT GCTTATGGAT TTCCTCAATG AATTCTATGC TCATCCCCGG 
CTTCAGGACC ATTATGTTGT TCTCCTGTGC CCCACTGAGA TGGACCTGCC TGTGAGGAGA 
GTCCTGCAAA TTCCTCTCTG GTCCCAACGA GTCATCTATC TGCAGGGTTC TGCCCTCAAG 
GACCAGGATC TCATGAGAGC CAAAATTGAT GACGCAGAAG CCTGTTTTAT ACTCAGCAAT 
CGTTTCGAGC GAGACCGCAT CGCAGCTGAC CATCAGACCA TCCTCCGTGC CTGGGCCGTG 
AAGGACTTCG CACCAAACTG TCCCCTCTAC GTTCAGATAC TCAAACCGGA AAACAAGTTT 
CACATCAAAT TTGCAGATCA TGTGGTCTGT GAAGAAGAAT TCAAGTACGC CATGTTGGCT 
CTAAATTGCG TCTGCCCAGC GACTTCCACC CTGATAACTC TGCTCATCCA CACCTCTAGG 
GGGCAAGACA GTGCCAGTTC TCCGGATCAG TGGCAGAACA CCTACAGCCG CTGCTCGGCC 
AATGAAGTTT ATCACATCCG ACTGAGTGAA AGCAAATTCT TTGGAGAGTA TGAAGGGAAA 
AGCTTTACCT ATTCATCCTT CCACGCACAT AAAAAGTACG GGGTCTGTTT GATTGGTGTC 
CGTCGGGATA ACAAAACCAG CATCCTGCTG AATCCAGGGC CTTGCCATAT AATGTCCTCA 
ACAGATACCT GCTTCTACAT AAATATCTCA AAAGAAGAGA ACTCGGCATT CAGTTTGAAA 
GACAACGAAG AAGCCAAGAA ATCCGGGTAC CCAAAGTCTA CCTACCATGG GCTGACAAGG 
CTCCCTGTTC ACAGCATTAT AGCAAGCATG GGAACAGTGG CCATGGATCT CCAAGGCTCC 
AGAAGCCCAG AGAGTGTGGA TGGGAATATG CTGAGCCCAC CTGCTGATGG AACTCATACA 
AGGAAACACA GTATAGACGC AGTGCTAGAT GTTGTGGATG GCGACACCAG TACAAATTTC 
GACCTTTTGA GTGACCAATC AGAAGATGAG ACCAGCCAGT CAGACGAGGA GCTGGATTCC 
TCCATCAACC TGGTTAAGGG ATACCCCTCA AACACACCGT ACATTGGCAG CTCACCCACT 
CTGTGCCACT TGCTCCAGCA GAAGAATCCA TATTGTTGCC TGCGTCTTGA CAAGAGCTGC 
CAACATTACC CATATGAAGA TGCAAGGGCA TATGGCTTTA GAAATGCACT GGTGATAGTG 
TCTGCAGAGC ATGCTGGTAA CGGGCTGTAT AACTTTATTG TTCCACTAAG AGCTTACTAC 
AGAGGCAAGA AGGAGCTAAG TCCCATCATC CTGCTTCTGG AGAACCCGCC AGATGTTCAG 
TTCCTGGAAG CCATTTGCTG GTTCCCTCTG GTCTACTACA TGGTTGGATC CATCGATAAT 
CTAGATGACA TGCTGCGCTG TGGGGTCAGT TACGCGGCAC ACATGGTAGT TGTTGGAGAG 
GAAAGCTCTA TGATTGCAGA AGAAGACTAC ATGGCTGACG CGCGCACTAT TGTCAATGTG 
CAGACTTTAT TTAGGCTGTT CCCAGGGTTG AACATCATCA CAGAGCTAAC GCATCCTGCA 
AACATGAGGT TCATGCAGTT CAAAGCCAAG GATAAATACT CCGTTGTACT CTCCAAGCTG 
GAGAAGAGGG AACGGGAACG GGGCTCGAAC CTGGTCTTCA TGTTCAGACT GCCCTTTGCC 
GCTGGGAAGG TTTTCAGCGT CAGCATGTTA GACACCCTCC TATACCAGTC ATTTGTTAAG 
GATTACATGA TCTCCATCAC ACGACTGCTG CTCGGCCTGG ATTCCATGCC TGGTTCTGGA 
TTCCTCTGTG CCCTCCGAGT GACAGAAGAA GACCTGTGGA TCCGTTGCTA TGGGCGGCTG 
TATCAGAAGC TCTGTTCCAC TACAGGCGAT ATTCCCATTG GGATATACCG TACTGAGACA 
CACTTGCTGC CTGCCTCTGA GTCTCAGGTA TCAATCAGTG TTGAAGATTA TGAAGATACA 
AGAGAGACTC GATGGGGAGA CCCTAGCCAG GGGCGACCCC TTCACCGGAA CTCAACCTCC 
AGTGACCAAT CAGAGCACCC CTTACTGCGC AGAAAGAGCA TGCAGTGGGC CAGAAGGCTA 
AGCCGCAAGG GAGCAAGGCA GCCTGGGAAA TCGGCTTCGG AACGAATCAG CCAGCAGCGA 
ATGAGTCTGT ACCGGCGGTC GGAGCGGCAG GAACTCAATG CACTGGTCAA GACCAGGATG 
AAGCATTTGG GGCTCTCCAC TGCTGAGTAC AGTATGAAAC AGATGGACAT TATGGACACT 
GACAACACTT TGTCCTATAT CCTAATAAAC CCATCTCCAG ATACCAAACT GGAACTTAAT 
GATGTTGTTT ACCTCATCCG ACCAGACCCT CTTACTCTGG TGTCTATGAA GTCCCGTAAA 
GCTAGCGTCA CACGCTCGGA AGGCACGGTA TACGACATGA AGGACAGCAC CCATCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012808571.1
CDS	2411..5890
Translation	MELEKDVQPLPPRYRFRDLLMGDWQQDDRVHVEFYVNENSFKERLKLFFIKNQRSSLRVRVFNCSLKLLSCLLYIIRVLLDDPRESRGGCWECTKQNYSAQQKFDWTPIIWVNRPLPLWGLQVLVALISFWETMLLTYLGYKGNIWEQLLRIPFILEMLNTLPFFVTIFWPPLRNVFIPVFLNCWLAKHALENMINDLHRAIQRTHSAMFNQVFILICTLICLMFTCICGIQHLERAGNNLTLFDSLYFCIVTFSTVGFGDVTPKIWPSQLLVVIMICVALIVLPIQFEQLVFLWMERQKSGGNYSRYRAQTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQDHYVVLLCPTEMDLPVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKIDDAEACFILSNRFERDRIAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHIKFADHVVCEEEFKYAMLALNCVCPATSTLITLLIHTSRGQDSASSPDQWQNTYSRCSANEVYHIRLSESKFFGEYEGKSFTYSSFHAHKKYGVCLIGVRRDNKTSILLNPGPCHIMSSTDTCFYINISKEENSAFSLKDNEEAKKSGYPKSTYHGLTRLPVHSIIASMGTVAMDLQGSRSPESVDGNMLSPPADGTHTRKHSIDAVLDVVDGDTSTNFDLLSDQSEDETSQSDEELDSSINLVKGYPSNTPYIGSSPTLCHLLQQKNPYCCLRLDKSCQHYPYEDARAYGFRNALVIVSAEHAGNGLYNFIVPLRAYYRGKKELSPIILLLENPPDVQFLEAICWFPLVYYMVGSIDNLDDMLRCGVSYAAHMVVVGEESSMIAEEDYMADARTIVNVQTLFRLFPGLNIITELTHPANMRFMQFKAKDKYSVVLSKLEKRERERGSNLVFMFRLPFAAGKVFSVSMLDTLLYQSFVKDYMISITRLLLGLDSMPGSGFLCALRVTEEDLWIRCYGRLYQKLCSTTGDIPIGIYRTETHLLPASESQVSISVEDYEDTRETRWGDPSQGRPLHRNSTSSDQSEHPLLRRKSMQWARRLSRKGARQPGKSASERISQQRMSLYRRSERQELNALVKTRMKHLGLSTAEYSMKQMDIMDTDNTLSYILINPSPDTKLELNDVVYLIRPDPLTLVSMKSRKASVTRSEGTVYDMKDSTHL

Target ORF information:

RefSeq Version	XM_012953117.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis potassium channel, sodium activated subfamily T, member 1 (kcnt1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012953117.2

ORF Insert Sequence:

ATGGAGCTGG AGAAAGATGT GCAACCTTTG CCTCCACGCT ATCGATTTCG GGACTTGTTG 
ATGGGTGACT GGCAACAGGA TGACAGGGTC CATGTGGAAT TTTATGTCAA TGAAAACTCG 
TTTAAGGAAC GACTAAAGTT ATTTTTTATA AAGAATCAGA GATCAAGTTT GAGAGTCAGA 
GTCTTTAACT GTTCCTTAAA GTTACTGAGT TGCCTCCTCT ATATTATAAG GGTCCTTCTG 
GATGATCCGA GAGAGAGCAG GGGCGGATGC TGGGAATGTA CCAAACAGAA TTACTCTGCA 
CAACAGAAAT TTGACTGGAC CCCAATTATT TGGGTAAATA GACCTCTTCC CCTGTGGGGG 
CTTCAGGTAC TCGTGGCGCT AATTAGCTTC TGGGAAACAA TGCTTCTCAC CTACCTTGGC 
TACAAGGGAA ACATATGGGA GCAGCTCCTC CGGATCCCCT TCATTCTAGA GATGCTGAAC 
ACCCTGCCTT TCTTTGTCAC GATTTTCTGG CCTCCGTTGA GGAATGTCTT CATTCCTGTT 
TTCCTCAATT GCTGGTTGGC TAAACATGCG CTGGAGAACA TGATAAATGA CTTGCACCGA 
GCCATTCAGA GGACACACTC GGCCATGTTT AACCAGGTCT TTATTCTGAT ATGCACCCTC 
ATCTGCCTCA TGTTCACTTG CATCTGTGGG ATTCAGCATC TGGAGAGAGC GGGCAATAAC 
CTCACCCTCT TTGACTCCCT GTATTTCTGC ATTGTCACTT TCTCCACGGT GGGCTTCGGA 
GACGTCACCC CAAAGATCTG GCCGTCACAG CTCCTGGTTG TCATTATGAT CTGTGTGGCA 
CTCATTGTGC TGCCCATCCA GTTTGAGCAG CTCGTCTTCC TATGGATGGA AAGGCAGAAA 
TCTGGCGGGA ATTACAGCCG ATACCGAGCG CAGACGGAGA AGCACGTGGT CCTGTGCGTC 
AGTTCTTTGA AGATTGACCT GCTTATGGAT TTCCTCAATG AATTCTATGC TCATCCCCGG 
CTTCAGGACC ATTATGTTGT TCTCCTGTGC CCCACTGAGA TGGACCTGCC TGTGAGGAGA 
GTCCTGCAAA TTCCTCTCTG GTCCCAACGA GTCATCTATC TGCAGGGTTC TGCCCTCAAG 
GACCAGGATC TCATGAGAGC CAAAATTGAT GACGCAGAAG CCTGTTTTAT ACTCAGCAAT 
CGTTTCGAGC GAGACCGCAT CGCAGCTGAC CATCAGACCA TCCTCCGTGC CTGGGCCGTG 
AAGGACTTCG CACCAAACTG TCCCCTCTAC GTTCAGATAC TCAAACCGGA AAACAAGTTT 
CACATCAAAT TTGCAGATCA TGTGGTCTGT GAAGAAGAAT TCAAGTACGC CATGTTGGCT 
CTAAATTGCG TCTGCCCAGC GACTTCCACC CTGATAACTC TGCTCATCCA CACCTCTAGG 
GGGCAAGACA GTGCCAGTTC TCCGGATCAG TGGCAGAACA CCTACAGCCG CTGCTCGGCC 
AATGAAGTTT ATCACATCCG ACTGAGTGAA AGCAAATTCT TTGGAGAGTA TGAAGGGAAA 
AGCTTTACCT ATTCATCCTT CCACGCACAT AAAAAGTACG GGGTCTGTTT GATTGGTGTC 
CGTCGGGATA ACAAAACCAG CATCCTGCTG AATCCAGGGC CTTGCCATAT AATGTCCTCA 
ACAGATACCT GCTTCTACAT AAATATCTCA AAAGAAGAGA ACTCGGCATT CAGTTTGAAA 
GACAACGAAG AAGCCAAGAA ATCCGGGTAC CCAAAGTCTA CCTACCATGG GCTGACAAGG 
CTCCCTGTTC ACAGCATTAT AGCAAGCATG GGAACAGTGG CCATGGATCT CCAAGGCTCC 
AGAAGCCCAG AGAGTGTGGA TGGGAATATG CTGAGCCCAC CTGCTGATGG AACTCATACA 
AGGAAACACA GTATAGACGC AGTGCTAGAT GTTGTGGATG GCGACACCAG TACAAATTTC 
GACCTTTTGA GTGACCAATC AGAAGATGAG ACCAGCCAGT CAGACGAGGA GCTGGATTCC 
TCCATCAACC TGGTTAAGGG ATACCCCTCA AACACACCGT ACATTGGCAG CTCACCCACT 
CTGTGCCACT TGCTCCAGCA GAAGAATCCA TATTGTTGCC TGCGTCTTGA CAAGAGCTGC 
CAACATTACC CATATGAAGA TGCAAGGGCA TATGGCTTTA GAAATGCACT GGTGATAGTG 
TCTGCAGAGC ATGCTGGTAA CGGGCTGTAT AACTTTATTG TTCCACTAAG AGCTTACTAC 
AGAGGCAAGA AGGAGCTAAG TCCCATCATC CTGCTTCTGG AGAACCCGCC AGATGTTCAG 
TTCCTGGAAG CCATTTGCTG GTTCCCTCTG GTCTACTACA TGGTTGGATC CATCGATAAT 
CTAGATGACA TGCTGCGCTG TGGGGTCAGT TACGCGGCAC ACATGGTAGT TGTTGGAGAG 
GAAAGCTCTA TGATTGCAGA AGAAGACTAC ATGGCTGACG CGCGCACTAT TGTCAATGTG 
CAGACTTTAT TTAGGCTGTT CCCAGGGTTG AACATCATCA CAGAGCTAAC GCATCCTGCA 
AACATGAGGT TCATGCAGTT CAAAGCCAAG GATAAATACT CCGTTGTACT CTCCAAGCTG 
GAGAAGAGGG AACGGGAACG GGGCTCGAAC CTGGTCTTCA TGTTCAGACT GCCCTTTGCC 
GCTGGGAAGG TTTTCAGCGT CAGCATGTTA GACACCCTCC TATACCAGTC ATTTGTTAAG 
GATTACATGA TCTCCATCAC ACGACTGCTG CTCGGCCTGG ATTCCATGCC TGGTTCTGGA 
TTCCTCTGTG CCCTCCGAGT GACAGAAGAA GACCTGTGGA TCCGTTGCTA TGGGCGGCTG 
TATCAGAAGC TCTGTTCCAC TACAGGCGAT ATTCCCATTG GGATATACCG TACTGAGACA 
CACTTGCTGC CTGCCTCTGA GTCTCAGGTA TCAATCAGTG TTGAAGATTA TGAAGATACA 
AGAGAGACTC GATGGGGAGA CCCTAGCCAG GGGCGACCCC TTCACCGGAA CTCAACCTCC 
AGTGACCAAT CAGAGCACCC CTTACTGCGC AGAAAGAGCA TGCAGTGGGC CAGAAGGCTA 
AGCCGCAAGG GAGCAAGGCA GCCTGGGAAA TCGGCTTCGG AACGAATCAG CCAGCAGCGA 
ATGAGTCTGT ACCGGCGGTC GGAGCGGCAG GAACTCAATG CACTGGTCAA GACCAGGATG 
AAGCATTTGG GGCTCTCCAC TGCTGAGTAC AGTATGAAAC AGATGGACAT TATGGACACT 
GACAACACTT TGTCCTATAT CCTAATAAAC CCATCTCCAG ATACCAAACT GGAACTTAAT 
GATGTTGTTT ACCTCATCCG ACCAGACCCT CTTACTCTGG TGTCTATGAA GTCCCGTAAA 
GCTAGCGTCA CACGCTCGGA AGGCACGGTA TACGACATGA AGGACAGCAC CCATCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kcnt1 ( XM_012953119.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012808573.1 Xenopus tropicalis potassium channel, sodium activated subfamily T, member 2 like (kcnt2l), transcript variant X2, mRNA.

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CloneID	OXa29210
Clone ID Related Accession (Same CDS sequence)	XM_012953119.3 , XM_012953119.2
Accession Version	XM_012953119.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	potassium channel subfamily T member 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012953119.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGG AGAAAGATGT GCAACCTTTG CCTCCACGCT ATCGATTTCG GGACTTGTTG 
ATGGGTGACT GGCAACAGGA TGACAGGGTC CATGTGGAAT TTTATGTCAA TGAAAACTCG 
TTTAAGGAAC GACTAAAGTT ATTTTTTATA AAGAATCAGA GATCAAGTTT GAGAGTCAGA 
GTCTTTAACT GTTCCTTAAA GTTACTGAGT TGCCTCCTCT ATATTATAAG GGTCCTTCTG 
GATGATCCGA GAGAGAGCAG GGGCGGATGC TGGGAATGTA CCAAACAGAA TTACTCTGCA 
CAACAGAAAT TTGACTGGAC CCCAATTATT TGGGTAAATA GACCTCTTCC CCTGTGGGGG 
CTTCAGGTAC TCGTGGCGCT AATTAGCTTC TGGGAAACAA TGCTTCTCAC CTACCTTGGC 
TACAAGGGAA ACATATGGGA GCAGCTCCTC CGGATCCCCT TCATTCTAGA GATGCTGAAC 
ACCCTGCCTT TCTTTGTCAC GATTTTCTGG CCTCCGTTGA GGAATGTCTT CATTCCTGTT 
TTCCTCAATT GCTGGTTGGC TAAACATGCG CTGGAGAACA TGATAAATGA CTTGCACCGA 
GCCATTCAGA GGACACACTC GGCCATGTTT AACCAGGTCT TTATTCTGAT ATGCACCCTC 
ATCTGCCTCA TGTTCACTTG CATCTGTGGG ATTCAGCATC TGGAGAGAGC GGGCAATAAC 
CTCACCCTCT TTGACTCCCT GTATTTCTGC ATTGTCACTT TCTCCACGGT GGGCTTCGGA 
GACGTCACCC CAAAGATCTG GCCGTCACAG CTCCTGGTTG TCATTATGAT CTGTGTGGCA 
CTCATTGTGC TGCCCATCCA GTTTGAGCAG CTCGTCTTCC TATGGATGGA AAGGCAGAAA 
TCTGGCGGGA ATTACAGCCG ATACCGAGCG CAGACGGAGA AGCACGTGGT CCTGTGCGTC 
AGTTCTTTGA AGATTGACCT GCTTATGGAT TTCCTCAATG AATTCTATGC TCATCCCCGG 
CTTCAGGACC ATTATGTTGT TCTCCTGTGC CCCACTGAGA TGGACCTGCC TGTGAGGAGA 
GTCCTGCAAA TTCCTCTCTG GTCCCAACGA GTCATCTATC TGCAGGGTTC TGCCCTCAAG 
GACCAGGATC TCATGAGAGC CAAAATTGAT GACGCAGAAG CCTGTTTTAT ACTCAGCAAT 
CGTTTCGAGC GAGACCGCAT CGCAGCTGAC CATCAGACCA TCCTCCGTGC CTGGGCCGTG 
AAGGACTTCG CACCAAACTG TCCCCTCTAC GTTCAGATAC TCAAACCGGA AAACAAGTTT 
CACATCAAAT TTGCAGATCA TGTGGTCTGT GAAGAAGAAT TCAAGTACGC CATGTTGGCT 
CTAAATTGCG TCTGCCCAGC GACTTCCACC CTGATAACTC TGCTCATCCA CACCTCTAGG 
GGGCAAGACA GTGCCAGTTC TCCGGATCAG TGGCAGAACA CCTACAGCCG CTGCTCGGCC 
AATGAAGTTT ATCACATCCG ACTGAGTGAA AGCAAATTCT TTGGAGAGTA TGAAGGGAAA 
AGCTTTACCT ATTCATCCTT CCACGCACAT AAAAAGTACG GGGTCTGTTT GATTGGTGTC 
CGTCGGGATA ACAAAACCAG CATCCTGCTG AATCCAGGGC CTTGCCATAT AATGTCCTCA 
ACAGATACCT GCTTCTACAT AAATATCTCA AAAGAAGAGA ACTCGGCATT CAGTTTGAAA 
GACAACGAAG AAGCCAAGAA ATCCGGGTAC CCAAAGTCTA CCTACCATGG GCTGACAAGG 
CTCCCTGTTC ACAGCATTAT AGCAAGCATG GGAACAGTGG CCATGGATCT CCAAGGCTCC 
AGAAGCCCAG AGAGTGTGGA TGGGAATATG CTGAGCCCAC CTGCTGATGG AACTCATACA 
AGGAAACACA GTATAGACGC AGTGCTAGAT GTTGTGGATG GCGACACCAG TACAAATTTC 
GACCTTTTGA GTGACCAATC AGAAGATGAG ACCAGCCAGT CAGACGAGGA GCTGGATTCC 
TCCATCAACC TGGTTAAGGG ATACCCCTCA AACACACCGT ACATTGGCAG CTCACCCACT 
CTGTGCCACT TGCTCCAGCA GAAGAATCCA TATTGTTGCC TGCGTCTTGA CAAGAGCTGC 
CAACATTACC CATATGAAGA TGCAAGGGCA TATGGCTTTA GAAATGCACT GGTGATAGTG 
TCTGCAGAGC ATGCTGGTAA CGGGCTGTAT AACTTTATTG TTCCACTAAG AGCTTACTAC 
AGAGGCAAGA AGGAGCTAAG TCCCATCATC CTGCTTCTGG AGAACCCGCC AGATGTTCAG 
TTCCTGGAAG CCATTTGCTG GTTCCCTCTG GTCTACTACA TGGTTGGATC CATCGATAAT 
CTAGATGACA TGCTGCGCTG TGGGGTCAGT TACGCGGCAC ACATGGTAGT TGTTGGAGAG 
GAAAGCTCTA TGATTGCAGA AGAAGACTAC ATGGCTGACG CGCGCACTAT TGTCAATGTG 
CAGACTTTAT TTAGGCTGTT CCCAGGGTTG AACATCATCA CAGAGCTAAC GCATCCTGCA 
AACATGAGGT TCATGCAGTT CAAAGCCAAG GATAAATACT CCGTTGTACT CTCCAAGCTG 
GAGAAGAGGG AACGGGAACG GGGCTCGAAC CTGGTCTTCA TGTTCAGACT GCCCTTTGCC 
GCTGGGAAGG TTTTCAGCGT CAGCATGTTA GACACCCTCC TATACCAGTC ATTTGTTAAG 
GATTACATGA TCTCCATCAC ACGACTGCTG CTCGGCCTGG ATTCCATGCC TGGTTCTGGA 
TTCCTCTGTG CCCTCCGAGT GACAGAAGAA GACCTGTGGA TCCGTTGCTA TGGGCGGCTG 
TATCAGAAGC TCTGTTCCAC TACAGGCGAT ATTCCCATTG GGATATACCG TACTGAGACA 
CACTTGCTGC CTGCCTCTGA GTCTCAGGTA TCAATCAGTG TTGAAGATTA TGAAGATACA 
AGAGAGACTC GATGGGGAGA CCCTAGCCAG GGGCGACCCC TTCACCGGAA CTCAACCTCC 
AGTGACCAAT CAGAGCACCC CTTACTGCGC AGAAAGAGCA TGCAGTGGGC CAGAAGGCTA 
AGCCGCAAGG GAGCAAGGCA GCCTGGGAAA TCGGCTTCGG AACGAATCAG CCAGCAGCGA 
ATGAGTCTGT ACCGGCGGTC GGAGCGGCAG GAACTCAATG CACTGGTCAA GACCAGGATG 
AAGCATTTGG GGCTCTCCAC TGCTGAGTAC ATGGACATTA TGGACACTGA CAACACTTTG 
TCCTATATCC TAATAAACCC ATCTCCAGAT ACCAAACTGG AACTTAATGA TGTTGTTTAC 
CTCATCCGAC CAGACCCTCT TACTCTGGTG TCTATGAAGT CCCGTAAAGC TAGCGTCACA 
CGCTCGGAAG GCACGGTATA CGACATGAAG GACAGCACCC ATCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012808573.1
CDS	1474..4941
Translation	MELEKDVQPLPPRYRFRDLLMGDWQQDDRVHVEFYVNENSFKERLKLFFIKNQRSSLRVRVFNCSLKLLSCLLYIIRVLLDDPRESRGGCWECTKQNYSAQQKFDWTPIIWVNRPLPLWGLQVLVALISFWETMLLTYLGYKGNIWEQLLRIPFILEMLNTLPFFVTIFWPPLRNVFIPVFLNCWLAKHALENMINDLHRAIQRTHSAMFNQVFILICTLICLMFTCICGIQHLERAGNNLTLFDSLYFCIVTFSTVGFGDVTPKIWPSQLLVVIMICVALIVLPIQFEQLVFLWMERQKSGGNYSRYRAQTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQDHYVVLLCPTEMDLPVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKIDDAEACFILSNRFERDRIAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHIKFADHVVCEEEFKYAMLALNCVCPATSTLITLLIHTSRGQDSASSPDQWQNTYSRCSANEVYHIRLSESKFFGEYEGKSFTYSSFHAHKKYGVCLIGVRRDNKTSILLNPGPCHIMSSTDTCFYINISKEENSAFSLKDNEEAKKSGYPKSTYHGLTRLPVHSIIASMGTVAMDLQGSRSPESVDGNMLSPPADGTHTRKHSIDAVLDVVDGDTSTNFDLLSDQSEDETSQSDEELDSSINLVKGYPSNTPYIGSSPTLCHLLQQKNPYCCLRLDKSCQHYPYEDARAYGFRNALVIVSAEHAGNGLYNFIVPLRAYYRGKKELSPIILLLENPPDVQFLEAICWFPLVYYMVGSIDNLDDMLRCGVSYAAHMVVVGEESSMIAEEDYMADARTIVNVQTLFRLFPGLNIITELTHPANMRFMQFKAKDKYSVVLSKLEKRERERGSNLVFMFRLPFAAGKVFSVSMLDTLLYQSFVKDYMISITRLLLGLDSMPGSGFLCALRVTEEDLWIRCYGRLYQKLCSTTGDIPIGIYRTETHLLPASESQVSISVEDYEDTRETRWGDPSQGRPLHRNSTSSDQSEHPLLRRKSMQWARRLSRKGARQPGKSASERISQQRMSLYRRSERQELNALVKTRMKHLGLSTAEYMDIMDTDNTLSYILINPSPDTKLELNDVVYLIRPDPLTLVSMKSRKASVTRSEGTVYDMKDSTHL

Target ORF information:

RefSeq Version	XM_012953119.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis potassium channel, sodium activated subfamily T, member 2 like (kcnt2l), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012953119.3

ORF Insert Sequence:

ATGGAGCTGG AGAAAGATGT GCAACCTTTG CCTCCACGCT ATCGATTTCG GGACTTGTTG 
ATGGGTGACT GGCAACAGGA TGACAGGGTC CATGTGGAAT TTTATGTCAA TGAAAACTCG 
TTTAAGGAAC GACTAAAGTT ATTTTTTATA AAGAATCAGA GATCAAGTTT GAGAGTCAGA 
GTCTTTAACT GTTCCTTAAA GTTACTGAGT TGCCTCCTCT ATATTATAAG GGTCCTTCTG 
GATGATCCGA GAGAGAGCAG GGGCGGATGC TGGGAATGTA CCAAACAGAA TTACTCTGCA 
CAACAGAAAT TTGACTGGAC CCCAATTATT TGGGTAAATA GACCTCTTCC CCTGTGGGGG 
CTTCAGGTAC TCGTGGCGCT AATTAGCTTC TGGGAAACAA TGCTTCTCAC CTACCTTGGC 
TACAAGGGAA ACATATGGGA GCAGCTCCTC CGGATCCCCT TCATTCTAGA GATGCTGAAC 
ACCCTGCCTT TCTTTGTCAC GATTTTCTGG CCTCCGTTGA GGAATGTCTT CATTCCTGTT 
TTCCTCAATT GCTGGTTGGC TAAACATGCG CTGGAGAACA TGATAAATGA CTTGCACCGA 
GCCATTCAGA GGACACACTC GGCCATGTTT AACCAGGTCT TTATTCTGAT ATGCACCCTC 
ATCTGCCTCA TGTTCACTTG CATCTGTGGG ATTCAGCATC TGGAGAGAGC GGGCAATAAC 
CTCACCCTCT TTGACTCCCT GTATTTCTGC ATTGTCACTT TCTCCACGGT GGGCTTCGGA 
GACGTCACCC CAAAGATCTG GCCGTCACAG CTCCTGGTTG TCATTATGAT CTGTGTGGCA 
CTCATTGTGC TGCCCATCCA GTTTGAGCAG CTCGTCTTCC TATGGATGGA AAGGCAGAAA 
TCTGGCGGGA ATTACAGCCG ATACCGAGCG CAGACGGAGA AGCACGTGGT CCTGTGCGTC 
AGTTCTTTGA AGATTGACCT GCTTATGGAT TTCCTCAATG AATTCTATGC TCATCCCCGG 
CTTCAGGACC ATTATGTTGT TCTCCTGTGC CCCACTGAGA TGGACCTGCC TGTGAGGAGA 
GTCCTGCAAA TTCCTCTCTG GTCCCAACGA GTCATCTATC TGCAGGGTTC TGCCCTCAAG 
GACCAGGATC TCATGAGAGC CAAAATTGAT GACGCAGAAG CCTGTTTTAT ACTCAGCAAT 
CGTTTCGAGC GAGACCGCAT CGCAGCTGAC CATCAGACCA TCCTCCGTGC CTGGGCCGTG 
AAGGACTTCG CACCAAACTG TCCCCTCTAC GTTCAGATAC TCAAACCGGA AAACAAGTTT 
CACATCAAAT TTGCAGATCA TGTGGTCTGT GAAGAAGAAT TCAAGTACGC CATGTTGGCT 
CTAAATTGCG TCTGCCCAGC GACTTCCACC CTGATAACTC TGCTCATCCA CACCTCTAGG 
GGGCAAGACA GTGCCAGTTC TCCGGATCAG TGGCAGAACA CCTACAGCCG CTGCTCGGCC 
AATGAAGTTT ATCACATCCG ACTGAGTGAA AGCAAATTCT TTGGAGAGTA TGAAGGGAAA 
AGCTTTACCT ATTCATCCTT CCACGCACAT AAAAAGTACG GGGTCTGTTT GATTGGTGTC 
CGTCGGGATA ACAAAACCAG CATCCTGCTG AATCCAGGGC CTTGCCATAT AATGTCCTCA 
ACAGATACCT GCTTCTACAT AAATATCTCA AAAGAAGAGA ACTCGGCATT CAGTTTGAAA 
GACAACGAAG AAGCCAAGAA ATCCGGGTAC CCAAAGTCTA CCTACCATGG GCTGACAAGG 
CTCCCTGTTC ACAGCATTAT AGCAAGCATG GGAACAGTGG CCATGGATCT CCAAGGCTCC 
AGAAGCCCAG AGAGTGTGGA TGGGAATATG CTGAGCCCAC CTGCTGATGG AACTCATACA 
AGGAAACACA GTATAGACGC AGTGCTAGAT GTTGTGGATG GCGACACCAG TACAAATTTC 
GACCTTTTGA GTGACCAATC AGAAGATGAG ACCAGCCAGT CAGACGAGGA GCTGGATTCC 
TCCATCAACC TGGTTAAGGG ATACCCCTCA AACACACCGT ACATTGGCAG CTCACCCACT 
CTGTGCCACT TGCTCCAGCA GAAGAATCCA TATTGTTGCC TGCGTCTTGA CAAGAGCTGC 
CAACATTACC CATATGAAGA TGCAAGGGCA TATGGCTTTA GAAATGCACT GGTGATAGTG 
TCTGCAGAGC ATGCTGGTAA CGGGCTGTAT AACTTTATTG TTCCACTAAG AGCTTACTAC 
AGAGGCAAGA AGGAGCTAAG TCCCATCATC CTGCTTCTGG AGAACCCGCC AGATGTTCAG 
TTCCTGGAAG CCATTTGCTG GTTCCCTCTG GTCTACTACA TGGTTGGATC CATCGATAAT 
CTAGATGACA TGCTGCGCTG TGGGGTCAGT TACGCGGCAC ACATGGTAGT TGTTGGAGAG 
GAAAGCTCTA TGATTGCAGA AGAAGACTAC ATGGCTGACG CGCGCACTAT TGTCAATGTG 
CAGACTTTAT TTAGGCTGTT CCCAGGGTTG AACATCATCA CAGAGCTAAC GCATCCTGCA 
AACATGAGGT TCATGCAGTT CAAAGCCAAG GATAAATACT CCGTTGTACT CTCCAAGCTG 
GAGAAGAGGG AACGGGAACG GGGCTCGAAC CTGGTCTTCA TGTTCAGACT GCCCTTTGCC 
GCTGGGAAGG TTTTCAGCGT CAGCATGTTA GACACCCTCC TATACCAGTC ATTTGTTAAG 
GATTACATGA TCTCCATCAC ACGACTGCTG CTCGGCCTGG ATTCCATGCC TGGTTCTGGA 
TTCCTCTGTG CCCTCCGAGT GACAGAAGAA GACCTGTGGA TCCGTTGCTA TGGGCGGCTG 
TATCAGAAGC TCTGTTCCAC TACAGGCGAT ATTCCCATTG GGATATACCG TACTGAGACA 
CACTTGCTGC CTGCCTCTGA GTCTCAGGTA TCAATCAGTG TTGAAGATTA TGAAGATACA 
AGAGAGACTC GATGGGGAGA CCCTAGCCAG GGGCGACCCC TTCACCGGAA CTCAACCTCC 
AGTGACCAAT CAGAGCACCC CTTACTGCGC AGAAAGAGCA TGCAGTGGGC CAGAAGGCTA 
AGCCGCAAGG GAGCAAGGCA GCCTGGGAAA TCGGCTTCGG AACGAATCAG CCAGCAGCGA 
ATGAGTCTGT ACCGGCGGTC GGAGCGGCAG GAACTCAATG CACTGGTCAA GACCAGGATG 
AAGCATTTGG GGCTCTCCAC TGCTGAGTAC ATGGACATTA TGGACACTGA CAACACTTTG 
TCCTATATCC TAATAAACCC ATCTCCAGAT ACCAAACTGG AACTTAATGA TGTTGTTTAC 
CTCATCCGAC CAGACCCTCT TACTCTGGTG TCTATGAAGT CCCGTAAAGC TAGCGTCACA 
CGCTCGGAAG GCACGGTATA CGACATGAAG GACAGCACCC ATCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kcnt1 ( XM_012953119.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012808573.1 Xenopus tropicalis potassium channel, sodium activated subfamily T, member 1 (kcnt1), transcript variant X3, mRNA.

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CloneID	OXa29210
Clone ID Related Accession (Same CDS sequence)	XM_012953119.3 , XM_012953119.2
Accession Version	XM_012953119.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	potassium channel subfamily T member 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012953119.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGG AGAAAGATGT GCAACCTTTG CCTCCACGCT ATCGATTTCG GGACTTGTTG 
ATGGGTGACT GGCAACAGGA TGACAGGGTC CATGTGGAAT TTTATGTCAA TGAAAACTCG 
TTTAAGGAAC GACTAAAGTT ATTTTTTATA AAGAATCAGA GATCAAGTTT GAGAGTCAGA 
GTCTTTAACT GTTCCTTAAA GTTACTGAGT TGCCTCCTCT ATATTATAAG GGTCCTTCTG 
GATGATCCGA GAGAGAGCAG GGGCGGATGC TGGGAATGTA CCAAACAGAA TTACTCTGCA 
CAACAGAAAT TTGACTGGAC CCCAATTATT TGGGTAAATA GACCTCTTCC CCTGTGGGGG 
CTTCAGGTAC TCGTGGCGCT AATTAGCTTC TGGGAAACAA TGCTTCTCAC CTACCTTGGC 
TACAAGGGAA ACATATGGGA GCAGCTCCTC CGGATCCCCT TCATTCTAGA GATGCTGAAC 
ACCCTGCCTT TCTTTGTCAC GATTTTCTGG CCTCCGTTGA GGAATGTCTT CATTCCTGTT 
TTCCTCAATT GCTGGTTGGC TAAACATGCG CTGGAGAACA TGATAAATGA CTTGCACCGA 
GCCATTCAGA GGACACACTC GGCCATGTTT AACCAGGTCT TTATTCTGAT ATGCACCCTC 
ATCTGCCTCA TGTTCACTTG CATCTGTGGG ATTCAGCATC TGGAGAGAGC GGGCAATAAC 
CTCACCCTCT TTGACTCCCT GTATTTCTGC ATTGTCACTT TCTCCACGGT GGGCTTCGGA 
GACGTCACCC CAAAGATCTG GCCGTCACAG CTCCTGGTTG TCATTATGAT CTGTGTGGCA 
CTCATTGTGC TGCCCATCCA GTTTGAGCAG CTCGTCTTCC TATGGATGGA AAGGCAGAAA 
TCTGGCGGGA ATTACAGCCG ATACCGAGCG CAGACGGAGA AGCACGTGGT CCTGTGCGTC 
AGTTCTTTGA AGATTGACCT GCTTATGGAT TTCCTCAATG AATTCTATGC TCATCCCCGG 
CTTCAGGACC ATTATGTTGT TCTCCTGTGC CCCACTGAGA TGGACCTGCC TGTGAGGAGA 
GTCCTGCAAA TTCCTCTCTG GTCCCAACGA GTCATCTATC TGCAGGGTTC TGCCCTCAAG 
GACCAGGATC TCATGAGAGC CAAAATTGAT GACGCAGAAG CCTGTTTTAT ACTCAGCAAT 
CGTTTCGAGC GAGACCGCAT CGCAGCTGAC CATCAGACCA TCCTCCGTGC CTGGGCCGTG 
AAGGACTTCG CACCAAACTG TCCCCTCTAC GTTCAGATAC TCAAACCGGA AAACAAGTTT 
CACATCAAAT TTGCAGATCA TGTGGTCTGT GAAGAAGAAT TCAAGTACGC CATGTTGGCT 
CTAAATTGCG TCTGCCCAGC GACTTCCACC CTGATAACTC TGCTCATCCA CACCTCTAGG 
GGGCAAGACA GTGCCAGTTC TCCGGATCAG TGGCAGAACA CCTACAGCCG CTGCTCGGCC 
AATGAAGTTT ATCACATCCG ACTGAGTGAA AGCAAATTCT TTGGAGAGTA TGAAGGGAAA 
AGCTTTACCT ATTCATCCTT CCACGCACAT AAAAAGTACG GGGTCTGTTT GATTGGTGTC 
CGTCGGGATA ACAAAACCAG CATCCTGCTG AATCCAGGGC CTTGCCATAT AATGTCCTCA 
ACAGATACCT GCTTCTACAT AAATATCTCA AAAGAAGAGA ACTCGGCATT CAGTTTGAAA 
GACAACGAAG AAGCCAAGAA ATCCGGGTAC CCAAAGTCTA CCTACCATGG GCTGACAAGG 
CTCCCTGTTC ACAGCATTAT AGCAAGCATG GGAACAGTGG CCATGGATCT CCAAGGCTCC 
AGAAGCCCAG AGAGTGTGGA TGGGAATATG CTGAGCCCAC CTGCTGATGG AACTCATACA 
AGGAAACACA GTATAGACGC AGTGCTAGAT GTTGTGGATG GCGACACCAG TACAAATTTC 
GACCTTTTGA GTGACCAATC AGAAGATGAG ACCAGCCAGT CAGACGAGGA GCTGGATTCC 
TCCATCAACC TGGTTAAGGG ATACCCCTCA AACACACCGT ACATTGGCAG CTCACCCACT 
CTGTGCCACT TGCTCCAGCA GAAGAATCCA TATTGTTGCC TGCGTCTTGA CAAGAGCTGC 
CAACATTACC CATATGAAGA TGCAAGGGCA TATGGCTTTA GAAATGCACT GGTGATAGTG 
TCTGCAGAGC ATGCTGGTAA CGGGCTGTAT AACTTTATTG TTCCACTAAG AGCTTACTAC 
AGAGGCAAGA AGGAGCTAAG TCCCATCATC CTGCTTCTGG AGAACCCGCC AGATGTTCAG 
TTCCTGGAAG CCATTTGCTG GTTCCCTCTG GTCTACTACA TGGTTGGATC CATCGATAAT 
CTAGATGACA TGCTGCGCTG TGGGGTCAGT TACGCGGCAC ACATGGTAGT TGTTGGAGAG 
GAAAGCTCTA TGATTGCAGA AGAAGACTAC ATGGCTGACG CGCGCACTAT TGTCAATGTG 
CAGACTTTAT TTAGGCTGTT CCCAGGGTTG AACATCATCA CAGAGCTAAC GCATCCTGCA 
AACATGAGGT TCATGCAGTT CAAAGCCAAG GATAAATACT CCGTTGTACT CTCCAAGCTG 
GAGAAGAGGG AACGGGAACG GGGCTCGAAC CTGGTCTTCA TGTTCAGACT GCCCTTTGCC 
GCTGGGAAGG TTTTCAGCGT CAGCATGTTA GACACCCTCC TATACCAGTC ATTTGTTAAG 
GATTACATGA TCTCCATCAC ACGACTGCTG CTCGGCCTGG ATTCCATGCC TGGTTCTGGA 
TTCCTCTGTG CCCTCCGAGT GACAGAAGAA GACCTGTGGA TCCGTTGCTA TGGGCGGCTG 
TATCAGAAGC TCTGTTCCAC TACAGGCGAT ATTCCCATTG GGATATACCG TACTGAGACA 
CACTTGCTGC CTGCCTCTGA GTCTCAGGTA TCAATCAGTG TTGAAGATTA TGAAGATACA 
AGAGAGACTC GATGGGGAGA CCCTAGCCAG GGGCGACCCC TTCACCGGAA CTCAACCTCC 
AGTGACCAAT CAGAGCACCC CTTACTGCGC AGAAAGAGCA TGCAGTGGGC CAGAAGGCTA 
AGCCGCAAGG GAGCAAGGCA GCCTGGGAAA TCGGCTTCGG AACGAATCAG CCAGCAGCGA 
ATGAGTCTGT ACCGGCGGTC GGAGCGGCAG GAACTCAATG CACTGGTCAA GACCAGGATG 
AAGCATTTGG GGCTCTCCAC TGCTGAGTAC ATGGACATTA TGGACACTGA CAACACTTTG 
TCCTATATCC TAATAAACCC ATCTCCAGAT ACCAAACTGG AACTTAATGA TGTTGTTTAC 
CTCATCCGAC CAGACCCTCT TACTCTGGTG TCTATGAAGT CCCGTAAAGC TAGCGTCACA 
CGCTCGGAAG GCACGGTATA CGACATGAAG GACAGCACCC ATCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012808573.1
CDS	2411..5878
Translation	MELEKDVQPLPPRYRFRDLLMGDWQQDDRVHVEFYVNENSFKERLKLFFIKNQRSSLRVRVFNCSLKLLSCLLYIIRVLLDDPRESRGGCWECTKQNYSAQQKFDWTPIIWVNRPLPLWGLQVLVALISFWETMLLTYLGYKGNIWEQLLRIPFILEMLNTLPFFVTIFWPPLRNVFIPVFLNCWLAKHALENMINDLHRAIQRTHSAMFNQVFILICTLICLMFTCICGIQHLERAGNNLTLFDSLYFCIVTFSTVGFGDVTPKIWPSQLLVVIMICVALIVLPIQFEQLVFLWMERQKSGGNYSRYRAQTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQDHYVVLLCPTEMDLPVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKIDDAEACFILSNRFERDRIAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHIKFADHVVCEEEFKYAMLALNCVCPATSTLITLLIHTSRGQDSASSPDQWQNTYSRCSANEVYHIRLSESKFFGEYEGKSFTYSSFHAHKKYGVCLIGVRRDNKTSILLNPGPCHIMSSTDTCFYINISKEENSAFSLKDNEEAKKSGYPKSTYHGLTRLPVHSIIASMGTVAMDLQGSRSPESVDGNMLSPPADGTHTRKHSIDAVLDVVDGDTSTNFDLLSDQSEDETSQSDEELDSSINLVKGYPSNTPYIGSSPTLCHLLQQKNPYCCLRLDKSCQHYPYEDARAYGFRNALVIVSAEHAGNGLYNFIVPLRAYYRGKKELSPIILLLENPPDVQFLEAICWFPLVYYMVGSIDNLDDMLRCGVSYAAHMVVVGEESSMIAEEDYMADARTIVNVQTLFRLFPGLNIITELTHPANMRFMQFKAKDKYSVVLSKLEKRERERGSNLVFMFRLPFAAGKVFSVSMLDTLLYQSFVKDYMISITRLLLGLDSMPGSGFLCALRVTEEDLWIRCYGRLYQKLCSTTGDIPIGIYRTETHLLPASESQVSISVEDYEDTRETRWGDPSQGRPLHRNSTSSDQSEHPLLRRKSMQWARRLSRKGARQPGKSASERISQQRMSLYRRSERQELNALVKTRMKHLGLSTAEYMDIMDTDNTLSYILINPSPDTKLELNDVVYLIRPDPLTLVSMKSRKASVTRSEGTVYDMKDSTHL

Target ORF information:

RefSeq Version	XM_012953119.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis potassium channel, sodium activated subfamily T, member 1 (kcnt1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012953119.2

ORF Insert Sequence:

ATGGAGCTGG AGAAAGATGT GCAACCTTTG CCTCCACGCT ATCGATTTCG GGACTTGTTG 
ATGGGTGACT GGCAACAGGA TGACAGGGTC CATGTGGAAT TTTATGTCAA TGAAAACTCG 
TTTAAGGAAC GACTAAAGTT ATTTTTTATA AAGAATCAGA GATCAAGTTT GAGAGTCAGA 
GTCTTTAACT GTTCCTTAAA GTTACTGAGT TGCCTCCTCT ATATTATAAG GGTCCTTCTG 
GATGATCCGA GAGAGAGCAG GGGCGGATGC TGGGAATGTA CCAAACAGAA TTACTCTGCA 
CAACAGAAAT TTGACTGGAC CCCAATTATT TGGGTAAATA GACCTCTTCC CCTGTGGGGG 
CTTCAGGTAC TCGTGGCGCT AATTAGCTTC TGGGAAACAA TGCTTCTCAC CTACCTTGGC 
TACAAGGGAA ACATATGGGA GCAGCTCCTC CGGATCCCCT TCATTCTAGA GATGCTGAAC 
ACCCTGCCTT TCTTTGTCAC GATTTTCTGG CCTCCGTTGA GGAATGTCTT CATTCCTGTT 
TTCCTCAATT GCTGGTTGGC TAAACATGCG CTGGAGAACA TGATAAATGA CTTGCACCGA 
GCCATTCAGA GGACACACTC GGCCATGTTT AACCAGGTCT TTATTCTGAT ATGCACCCTC 
ATCTGCCTCA TGTTCACTTG CATCTGTGGG ATTCAGCATC TGGAGAGAGC GGGCAATAAC 
CTCACCCTCT TTGACTCCCT GTATTTCTGC ATTGTCACTT TCTCCACGGT GGGCTTCGGA 
GACGTCACCC CAAAGATCTG GCCGTCACAG CTCCTGGTTG TCATTATGAT CTGTGTGGCA 
CTCATTGTGC TGCCCATCCA GTTTGAGCAG CTCGTCTTCC TATGGATGGA AAGGCAGAAA 
TCTGGCGGGA ATTACAGCCG ATACCGAGCG CAGACGGAGA AGCACGTGGT CCTGTGCGTC 
AGTTCTTTGA AGATTGACCT GCTTATGGAT TTCCTCAATG AATTCTATGC TCATCCCCGG 
CTTCAGGACC ATTATGTTGT TCTCCTGTGC CCCACTGAGA TGGACCTGCC TGTGAGGAGA 
GTCCTGCAAA TTCCTCTCTG GTCCCAACGA GTCATCTATC TGCAGGGTTC TGCCCTCAAG 
GACCAGGATC TCATGAGAGC CAAAATTGAT GACGCAGAAG CCTGTTTTAT ACTCAGCAAT 
CGTTTCGAGC GAGACCGCAT CGCAGCTGAC CATCAGACCA TCCTCCGTGC CTGGGCCGTG 
AAGGACTTCG CACCAAACTG TCCCCTCTAC GTTCAGATAC TCAAACCGGA AAACAAGTTT 
CACATCAAAT TTGCAGATCA TGTGGTCTGT GAAGAAGAAT TCAAGTACGC CATGTTGGCT 
CTAAATTGCG TCTGCCCAGC GACTTCCACC CTGATAACTC TGCTCATCCA CACCTCTAGG 
GGGCAAGACA GTGCCAGTTC TCCGGATCAG TGGCAGAACA CCTACAGCCG CTGCTCGGCC 
AATGAAGTTT ATCACATCCG ACTGAGTGAA AGCAAATTCT TTGGAGAGTA TGAAGGGAAA 
AGCTTTACCT ATTCATCCTT CCACGCACAT AAAAAGTACG GGGTCTGTTT GATTGGTGTC 
CGTCGGGATA ACAAAACCAG CATCCTGCTG AATCCAGGGC CTTGCCATAT AATGTCCTCA 
ACAGATACCT GCTTCTACAT AAATATCTCA AAAGAAGAGA ACTCGGCATT CAGTTTGAAA 
GACAACGAAG AAGCCAAGAA ATCCGGGTAC CCAAAGTCTA CCTACCATGG GCTGACAAGG 
CTCCCTGTTC ACAGCATTAT AGCAAGCATG GGAACAGTGG CCATGGATCT CCAAGGCTCC 
AGAAGCCCAG AGAGTGTGGA TGGGAATATG CTGAGCCCAC CTGCTGATGG AACTCATACA 
AGGAAACACA GTATAGACGC AGTGCTAGAT GTTGTGGATG GCGACACCAG TACAAATTTC 
GACCTTTTGA GTGACCAATC AGAAGATGAG ACCAGCCAGT CAGACGAGGA GCTGGATTCC 
TCCATCAACC TGGTTAAGGG ATACCCCTCA AACACACCGT ACATTGGCAG CTCACCCACT 
CTGTGCCACT TGCTCCAGCA GAAGAATCCA TATTGTTGCC TGCGTCTTGA CAAGAGCTGC 
CAACATTACC CATATGAAGA TGCAAGGGCA TATGGCTTTA GAAATGCACT GGTGATAGTG 
TCTGCAGAGC ATGCTGGTAA CGGGCTGTAT AACTTTATTG TTCCACTAAG AGCTTACTAC 
AGAGGCAAGA AGGAGCTAAG TCCCATCATC CTGCTTCTGG AGAACCCGCC AGATGTTCAG 
TTCCTGGAAG CCATTTGCTG GTTCCCTCTG GTCTACTACA TGGTTGGATC CATCGATAAT 
CTAGATGACA TGCTGCGCTG TGGGGTCAGT TACGCGGCAC ACATGGTAGT TGTTGGAGAG 
GAAAGCTCTA TGATTGCAGA AGAAGACTAC ATGGCTGACG CGCGCACTAT TGTCAATGTG 
CAGACTTTAT TTAGGCTGTT CCCAGGGTTG AACATCATCA CAGAGCTAAC GCATCCTGCA 
AACATGAGGT TCATGCAGTT CAAAGCCAAG GATAAATACT CCGTTGTACT CTCCAAGCTG 
GAGAAGAGGG AACGGGAACG GGGCTCGAAC CTGGTCTTCA TGTTCAGACT GCCCTTTGCC 
GCTGGGAAGG TTTTCAGCGT CAGCATGTTA GACACCCTCC TATACCAGTC ATTTGTTAAG 
GATTACATGA TCTCCATCAC ACGACTGCTG CTCGGCCTGG ATTCCATGCC TGGTTCTGGA 
TTCCTCTGTG CCCTCCGAGT GACAGAAGAA GACCTGTGGA TCCGTTGCTA TGGGCGGCTG 
TATCAGAAGC TCTGTTCCAC TACAGGCGAT ATTCCCATTG GGATATACCG TACTGAGACA 
CACTTGCTGC CTGCCTCTGA GTCTCAGGTA TCAATCAGTG TTGAAGATTA TGAAGATACA 
AGAGAGACTC GATGGGGAGA CCCTAGCCAG GGGCGACCCC TTCACCGGAA CTCAACCTCC 
AGTGACCAAT CAGAGCACCC CTTACTGCGC AGAAAGAGCA TGCAGTGGGC CAGAAGGCTA 
AGCCGCAAGG GAGCAAGGCA GCCTGGGAAA TCGGCTTCGG AACGAATCAG CCAGCAGCGA 
ATGAGTCTGT ACCGGCGGTC GGAGCGGCAG GAACTCAATG CACTGGTCAA GACCAGGATG 
AAGCATTTGG GGCTCTCCAC TGCTGAGTAC ATGGACATTA TGGACACTGA CAACACTTTG 
TCCTATATCC TAATAAACCC ATCTCCAGAT ACCAAACTGG AACTTAATGA TGTTGTTTAC 
CTCATCCGAC CAGACCCTCT TACTCTGGTG TCTATGAAGT CCCGTAAAGC TAGCGTCACA 
CGCTCGGAAG GCACGGTATA CGACATGAAG GACAGCACCC ATCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kcnt1 ( XM_031899402.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031755262.1 Xenopus tropicalis potassium channel, sodium activated subfamily T, member 2 like (kcnt2l), transcript variant X3, mRNA.

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CloneID	OXa29211
Clone ID Related Accession (Same CDS sequence)	XM_031899402.1
Accession Version	XM_031899402.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	potassium channel subfamily T member 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATC TAAAGTTCTT TGGGGGAGTG AATTTCCCCG GGCCAGCCGT GGATTCTGGG 
GTCCATGTGG AATTTTATGT CAATGAAAAC TCGTTTAAGG AACGACTAAA GTTATTTTTT 
ATAAAGAATC AGAGATCAAG TTTGAGAGTC AGAGTCTTTA ACTGTTCCTT AAAGTTACTG 
AGTTGCCTCC TCTATATTAT AAGGGTCCTT CTGGATGATC CGAGAGAGAG CAGGGGCGGA 
TGCTGGGAAT GTACCAAACA GAATTACTCT GCACAACAGA AATTTGACTG GACCCCAATT 
ATTTGGGTAA ATAGACCTCT TCCCCTGTGG GGGCTTCAGG TACTCGTGGC GCTAATTAGC 
TTCTGGGAAA CAATGCTTCT CACCTACCTT GGCTACAAGG GAAACATATG GGAGCAGCTC 
CTCCGGATCC CCTTCATTCT AGAGATGCTG AACACCCTGC CTTTCTTTGT CACGATTTTC 
TGGCCTCCGT TGAGGAATGT CTTCATTCCT GTTTTCCTCA ATTGCTGGTT GGCTAAACAT 
GCGCTGGAGA ACATGATAAA TGACTTGCAC CGAGCCATTC AGAGGACACA CTCGGCCATG 
TTTAACCAGG TCTTTATTCT GATATGCACC CTCATCTGCC TCATGTTCAC TTGCATCTGT 
GGGATTCAGC ATCTGGAGAG AGCGGGCAAT AACCTCACCC TCTTTGACTC CCTGTATTTC 
TGCATTGTCA CTTTCTCCAC GGTGGGCTTC GGAGACGTCA CCCCAAAGAT CTGGCCGTCA 
CAGCTCCTGG TTGTCATTAT GATCTGTGTG GCACTCATTG TGCTGCCCAT CCAGTTTGAG 
CAGCTCGTCT TCCTATGGAT GGAAAGGCAG AAATCTGGCG GGAATTACAG CCGATACCGA 
GCGCAGACGG AGAAGCACGT GGTCCTGTGC GTCAGTTCTT TGAAGATTGA CCTGCTTATG 
GATTTCCTCA ATGAATTCTA TGCTCATCCC CGGCTTCAGG ACCATTATGT TGTTCTCCTG 
TGCCCCACTG AGATGGACCT GCCTGTGAGG AGAGTCCTGC AAATTCCTCT CTGGTCCCAA 
CGAGTCATCT ATCTGCAGGG TTCTGCCCTC AAGGACCAGG ATCTCATGAG AGCCAAAATT 
GATGACGCAG AAGCCTGTTT TATACTCAGC AATCGTTTCG AGCGAGACCG CATCGCAGCT 
GACCATCAGA CCATCCTCCG TGCCTGGGCC GTGAAGGACT TCGCACCAAA CTGTCCCCTC 
TACGTTCAGA TACTCAAACC GGAAAACAAG TTTCACATCA AATTTGCAGA TCATGTGGTC 
TGTGAAGAAG AATTCAAGTA CGCCATGTTG GCTCTAAATT GCGTCTGCCC AGCGACTTCC 
ACCCTGATAA CTCTGCTCAT CCACACCTCT AGGGGGCAAG ACAGTGCCAG TTCTCCGGAT 
CAGTGGCAGA ACACCTACAG CCGCTGCTCG GCCAATGAAG TTTATCACAT CCGACTGAGT 
GAAAGCAAAT TCTTTGGAGA GTATGAAGGG AAAAGCTTTA CCTATTCATC CTTCCACGCA 
CATAAAAAGT ACGGGGTCTG TTTGATTGGT GTCCGTCGGG ATAACAAAAC CAGCATCCTG 
CTGAATCCAG GGCCTTGCCA TATAATGTCC TCAACAGATA CCTGCTTCTA CATAAATATC 
TCAAAAGAAG AGAACTCGGC ATTCAGTTTG AAAGACAACG AAGAAGCCAA GAAATCCGGG 
TACCCAAAGT CTACCTACCA TGGGCTGACA AGGCTCCCTG TTCACAGCAT TATAGCAAGC 
ATGGGAACAG TGGCCATGGA TCTCCAAGGC TCCAGAAGCC CAGAGAGTGT GGATGGGAAT 
ATGCTGAGCC CACCTGCTGA TGGAACTCAT ACAAGGAAAC ACAGTATAGA CGCAGTGCTA 
GATGTTGTGG ATGGCGACAC CAGTACAAAT TTCGACCTTT TGAGTGACCA ATCAGAAGAT 
GAGACCAGCC AGTCAGACGA GGAGCTGGAT TCCTCCATCA ACCTGGTTAA GGGATACCCC 
TCAAACACAC CGTACATTGG CAGCTCACCC ACTCTGTGCC ACTTGCTCCA GCAGAAGAAT 
CCATATTGTT GCCTGCGTCT TGACAAGAGC TGCCAACATT ACCCATATGA AGATGCAAGG 
GCATATGGCT TTAGAAATGC ACTGGTGATA GTGTCTGCAG AGCATGCTGG TAACGGGCTG 
TATAACTTTA TTGTTCCACT AAGAGCTTAC TACAGAGGCA AGAAGGAGCT AAGTCCCATC 
ATCCTGCTTC TGGAGAACCC GCCAGATGTT CAGTTCCTGG AAGCCATTTG CTGGTTCCCT 
CTGGTCTACT ACATGGTTGG ATCCATCGAT AATCTAGATG ACATGCTGCG CTGTGGGGTC 
AGTTACGCGG CACACATGGT AGTTGTTGGA GAGGAAAGCT CTATGATTGC AGAAGAAGAC 
TACATGGCTG ACGCGCGCAC TATTGTCAAT GTGCAGACTT TATTTAGGCT GTTCCCAGGG 
TTGAACATCA TCACAGAGCT AACGCATCCT GCAAACATGA GGTTCATGCA GTTCAAAGCC 
AAGGATAAAT ACTCCGTTGT ACTCTCCAAG CTGGAGAAGA GGGAACGGGA ACGGGGCTCG 
AACCTGGTCT TCATGTTCAG ACTGCCCTTT GCCGCTGGGA AGGTTTTCAG CGTCAGCATG 
TTAGACACCC TCCTATACCA GTCATTTGTT AAGGATTACA TGATCTCCAT CACACGACTG 
CTGCTCGGCC TGGATTCCAT GCCTGGTTCT GGATTCCTCT GTGCCCTCCG AGTGACAGAA 
GAAGACCTGT GGATCCGTTG CTATGGGCGG CTGTATCAGA AGCTCTGTTC CACTACAGGC 
GATATTCCCA TTGGGATATA CCGTACTGAG ACACACTTGC TGCCTGCCTC TGAGTCTCAG 
GTATCAATCA GTGTTGAAGA TTATGAAGAT ACAAGAGAGA CTCGATGGGG AGACCCTAGC 
CAGGGGCGAC CCCTTCACCG GAACTCAACC TCCAGTGACC AATCAGAGCA CCCCTTACTG 
CGCAGAAAGA GCATGCAGTG GGCCAGAAGG CTAAGCCGCA AGGGAGCAAG GCAGCCTGGG 
AAATCGGCTT CGGAACGAAT CAGCCAGCAG CGAATGAGTC TGTACCGGCG GTCGGAGCGG 
CAGGAACTCA ATGCACTGGT CAAGACCAGG ATGAAGCATT TGGGGCTCTC CACTGCTGAG 
TACAGTATGA AACAGATGGA CATTATGGAC ACTGACAACA CTTTGTCCTA TATCCTAATA 
AACCCATCTC CAGATACCAA ACTGGAACTT AATGATGTTG TTTACCTCAT CCGACCAGAC 
CCTCTTACTC TGGTGTCTAT GAAGTCCCGT AAAGCTAGCG TCACACGCTC GGAAGGCACG 
GTATACGACA TGAAGGACAG CACCCATCTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031755262.1
CDS	289..3741
Translation	MVDLKFFGGVNFPGPAVDSGVHVEFYVNENSFKERLKLFFIKNQRSSLRVRVFNCSLKLLSCLLYIIRVLLDDPRESRGGCWECTKQNYSAQQKFDWTPIIWVNRPLPLWGLQVLVALISFWETMLLTYLGYKGNIWEQLLRIPFILEMLNTLPFFVTIFWPPLRNVFIPVFLNCWLAKHALENMINDLHRAIQRTHSAMFNQVFILICTLICLMFTCICGIQHLERAGNNLTLFDSLYFCIVTFSTVGFGDVTPKIWPSQLLVVIMICVALIVLPIQFEQLVFLWMERQKSGGNYSRYRAQTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQDHYVVLLCPTEMDLPVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKIDDAEACFILSNRFERDRIAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHIKFADHVVCEEEFKYAMLALNCVCPATSTLITLLIHTSRGQDSASSPDQWQNTYSRCSANEVYHIRLSESKFFGEYEGKSFTYSSFHAHKKYGVCLIGVRRDNKTSILLNPGPCHIMSSTDTCFYINISKEENSAFSLKDNEEAKKSGYPKSTYHGLTRLPVHSIIASMGTVAMDLQGSRSPESVDGNMLSPPADGTHTRKHSIDAVLDVVDGDTSTNFDLLSDQSEDETSQSDEELDSSINLVKGYPSNTPYIGSSPTLCHLLQQKNPYCCLRLDKSCQHYPYEDARAYGFRNALVIVSAEHAGNGLYNFIVPLRAYYRGKKELSPIILLLENPPDVQFLEAICWFPLVYYMVGSIDNLDDMLRCGVSYAAHMVVVGEESSMIAEEDYMADARTIVNVQTLFRLFPGLNIITELTHPANMRFMQFKAKDKYSVVLSKLEKRERERGSNLVFMFRLPFAAGKVFSVSMLDTLLYQSFVKDYMISITRLLLGLDSMPGSGFLCALRVTEEDLWIRCYGRLYQKLCSTTGDIPIGIYRTETHLLPASESQVSISVEDYEDTRETRWGDPSQGRPLHRNSTSSDQSEHPLLRRKSMQWARRLSRKGARQPGKSASERISQQRMSLYRRSERQELNALVKTRMKHLGLSTAEYSMKQMDIMDTDNTLSYILINPSPDTKLELNDVVYLIRPDPLTLVSMKSRKASVTRSEGTVYDMKDSTHL

Target ORF information:

RefSeq Version	XM_031899402.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis potassium channel, sodium activated subfamily T, member 2 like (kcnt2l), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031899402.1

ORF Insert Sequence:

ATGGTGGATC TAAAGTTCTT TGGGGGAGTG AATTTCCCCG GGCCAGCCGT GGATTCTGGG 
GTCCATGTGG AATTTTATGT CAATGAAAAC TCGTTTAAGG AACGACTAAA GTTATTTTTT 
ATAAAGAATC AGAGATCAAG TTTGAGAGTC AGAGTCTTTA ACTGTTCCTT AAAGTTACTG 
AGTTGCCTCC TCTATATTAT AAGGGTCCTT CTGGATGATC CGAGAGAGAG CAGGGGCGGA 
TGCTGGGAAT GTACCAAACA GAATTACTCT GCACAACAGA AATTTGACTG GACCCCAATT 
ATTTGGGTAA ATAGACCTCT TCCCCTGTGG GGGCTTCAGG TACTCGTGGC GCTAATTAGC 
TTCTGGGAAA CAATGCTTCT CACCTACCTT GGCTACAAGG GAAACATATG GGAGCAGCTC 
CTCCGGATCC CCTTCATTCT AGAGATGCTG AACACCCTGC CTTTCTTTGT CACGATTTTC 
TGGCCTCCGT TGAGGAATGT CTTCATTCCT GTTTTCCTCA ATTGCTGGTT GGCTAAACAT 
GCGCTGGAGA ACATGATAAA TGACTTGCAC CGAGCCATTC AGAGGACACA CTCGGCCATG 
TTTAACCAGG TCTTTATTCT GATATGCACC CTCATCTGCC TCATGTTCAC TTGCATCTGT 
GGGATTCAGC ATCTGGAGAG AGCGGGCAAT AACCTCACCC TCTTTGACTC CCTGTATTTC 
TGCATTGTCA CTTTCTCCAC GGTGGGCTTC GGAGACGTCA CCCCAAAGAT CTGGCCGTCA 
CAGCTCCTGG TTGTCATTAT GATCTGTGTG GCACTCATTG TGCTGCCCAT CCAGTTTGAG 
CAGCTCGTCT TCCTATGGAT GGAAAGGCAG AAATCTGGCG GGAATTACAG CCGATACCGA 
GCGCAGACGG AGAAGCACGT GGTCCTGTGC GTCAGTTCTT TGAAGATTGA CCTGCTTATG 
GATTTCCTCA ATGAATTCTA TGCTCATCCC CGGCTTCAGG ACCATTATGT TGTTCTCCTG 
TGCCCCACTG AGATGGACCT GCCTGTGAGG AGAGTCCTGC AAATTCCTCT CTGGTCCCAA 
CGAGTCATCT ATCTGCAGGG TTCTGCCCTC AAGGACCAGG ATCTCATGAG AGCCAAAATT 
GATGACGCAG AAGCCTGTTT TATACTCAGC AATCGTTTCG AGCGAGACCG CATCGCAGCT 
GACCATCAGA CCATCCTCCG TGCCTGGGCC GTGAAGGACT TCGCACCAAA CTGTCCCCTC 
TACGTTCAGA TACTCAAACC GGAAAACAAG TTTCACATCA AATTTGCAGA TCATGTGGTC 
TGTGAAGAAG AATTCAAGTA CGCCATGTTG GCTCTAAATT GCGTCTGCCC AGCGACTTCC 
ACCCTGATAA CTCTGCTCAT CCACACCTCT AGGGGGCAAG ACAGTGCCAG TTCTCCGGAT 
CAGTGGCAGA ACACCTACAG CCGCTGCTCG GCCAATGAAG TTTATCACAT CCGACTGAGT 
GAAAGCAAAT TCTTTGGAGA GTATGAAGGG AAAAGCTTTA CCTATTCATC CTTCCACGCA 
CATAAAAAGT ACGGGGTCTG TTTGATTGGT GTCCGTCGGG ATAACAAAAC CAGCATCCTG 
CTGAATCCAG GGCCTTGCCA TATAATGTCC TCAACAGATA CCTGCTTCTA CATAAATATC 
TCAAAAGAAG AGAACTCGGC ATTCAGTTTG AAAGACAACG AAGAAGCCAA GAAATCCGGG 
TACCCAAAGT CTACCTACCA TGGGCTGACA AGGCTCCCTG TTCACAGCAT TATAGCAAGC 
ATGGGAACAG TGGCCATGGA TCTCCAAGGC TCCAGAAGCC CAGAGAGTGT GGATGGGAAT 
ATGCTGAGCC CACCTGCTGA TGGAACTCAT ACAAGGAAAC ACAGTATAGA CGCAGTGCTA 
GATGTTGTGG ATGGCGACAC CAGTACAAAT TTCGACCTTT TGAGTGACCA ATCAGAAGAT 
GAGACCAGCC AGTCAGACGA GGAGCTGGAT TCCTCCATCA ACCTGGTTAA GGGATACCCC 
TCAAACACAC CGTACATTGG CAGCTCACCC ACTCTGTGCC ACTTGCTCCA GCAGAAGAAT 
CCATATTGTT GCCTGCGTCT TGACAAGAGC TGCCAACATT ACCCATATGA AGATGCAAGG 
GCATATGGCT TTAGAAATGC ACTGGTGATA GTGTCTGCAG AGCATGCTGG TAACGGGCTG 
TATAACTTTA TTGTTCCACT AAGAGCTTAC TACAGAGGCA AGAAGGAGCT AAGTCCCATC 
ATCCTGCTTC TGGAGAACCC GCCAGATGTT CAGTTCCTGG AAGCCATTTG CTGGTTCCCT 
CTGGTCTACT ACATGGTTGG ATCCATCGAT AATCTAGATG ACATGCTGCG CTGTGGGGTC 
AGTTACGCGG CACACATGGT AGTTGTTGGA GAGGAAAGCT CTATGATTGC AGAAGAAGAC 
TACATGGCTG ACGCGCGCAC TATTGTCAAT GTGCAGACTT TATTTAGGCT GTTCCCAGGG 
TTGAACATCA TCACAGAGCT AACGCATCCT GCAAACATGA GGTTCATGCA GTTCAAAGCC 
AAGGATAAAT ACTCCGTTGT ACTCTCCAAG CTGGAGAAGA GGGAACGGGA ACGGGGCTCG 
AACCTGGTCT TCATGTTCAG ACTGCCCTTT GCCGCTGGGA AGGTTTTCAG CGTCAGCATG 
TTAGACACCC TCCTATACCA GTCATTTGTT AAGGATTACA TGATCTCCAT CACACGACTG 
CTGCTCGGCC TGGATTCCAT GCCTGGTTCT GGATTCCTCT GTGCCCTCCG AGTGACAGAA 
GAAGACCTGT GGATCCGTTG CTATGGGCGG CTGTATCAGA AGCTCTGTTC CACTACAGGC 
GATATTCCCA TTGGGATATA CCGTACTGAG ACACACTTGC TGCCTGCCTC TGAGTCTCAG 
GTATCAATCA GTGTTGAAGA TTATGAAGAT ACAAGAGAGA CTCGATGGGG AGACCCTAGC 
CAGGGGCGAC CCCTTCACCG GAACTCAACC TCCAGTGACC AATCAGAGCA CCCCTTACTG 
CGCAGAAAGA GCATGCAGTG GGCCAGAAGG CTAAGCCGCA AGGGAGCAAG GCAGCCTGGG 
AAATCGGCTT CGGAACGAAT CAGCCAGCAG CGAATGAGTC TGTACCGGCG GTCGGAGCGG 
CAGGAACTCA ATGCACTGGT CAAGACCAGG ATGAAGCATT TGGGGCTCTC CACTGCTGAG 
TACAGTATGA AACAGATGGA CATTATGGAC ACTGACAACA CTTTGTCCTA TATCCTAATA 
AACCCATCTC CAGATACCAA ACTGGAACTT AATGATGTTG TTTACCTCAT CCGACCAGAC 
CCTCTTACTC TGGTGTCTAT GAAGTCCCGT AAAGCTAGCG TCACACGCTC GGAAGGCACG 
GTATACGACA TGAAGGACAG CACCCATCTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kcnt1 ( XM_012953121.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012808575.1 Xenopus tropicalis potassium channel, sodium activated subfamily T, member 1 (kcnt1), transcript variant X4, mRNA.

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CloneID	OXa29212
Clone ID Related Accession (Same CDS sequence)	XM_012953121.2
Accession Version	XM_012953121.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	potassium channel subfamily T member 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012953121.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGG AGAAAGATGT GCAACCTTTG CCTCCACGCT ATCGATTTCG GGACTTGTTG 
ATGGGTGACT GGCAACAGGA TGACAGGGTC CATGTGGAAT TTTATGTCAA TGAAAACTCG 
TTTAAGGAAC GACTAAAGTT ATTTTTTATA AAGAATCAGA GATCAAGTTT GAGAGTCAGA 
GTCTTTAACT GTTCCTTAAA GTTACTGAGT TGCCTCCTCT ATATTATAAG GGTCCTTCTG 
GATGATCCGA GAGAGAGCAG GGGCGGATGC TGGGAATGTA CCAAACAGAA TTACTCTGCA 
CAACAGAAAT TTGACTGGAC CCCAATTATT TGGGTAAATA GACCTCTTCC CCTGTGGGGG 
CTTCAGGTAC TCGTGGCGCT AATTAGCTTC TGGGAAACAA TGCTTCTCAC CTACCTTGGC 
TACAAGGGAA ACATATGGGA GCAGCTCCTC CGGATCCCCT TCATTCTAGA GATGCTGAAC 
ACCCTGCCTT TCTTTGTCAC GATTTTCTGG CCTCCGTTGA GGAATGTCTT CATTCCTGTT 
TTCCTCAATT GCTGGTTGGC TAAACATGCG CTGGAGAACA TGATAAATGA CTTGCACCGA 
GCCATTCAGA GGACACACTC GGCCATGTTT AACCAGGTCT TTATTCTGAT ATGCACCCTC 
ATCTGCCTCA TGTTCACTTG CATCTGTGGG ATTCAGCATC TGGAGAGAGC GGGCAATAAC 
CTCACCCTCT TTGACTCCCT GTATTTCTGC ATTGTCACTT TCTCCACGGT GGGCTTCGGA 
GACGTCACCC CAAAGATCTG GCCGTCACAG CTCCTGGTTG TCATTATGAT CTGTGTGGCA 
CTCATTGTGC TGCCCATCCA GTTTGAGCAG CTCGTCTTCC TATGGATGGA AAGGCAGAAA 
TCTGGCGGGA ATTACAGCCG ATACCGAGCG CAGACGGAGA AGCACGTGGT CCTGTGCGTC 
AGTTCTTTGA AGATTGACCT GCTTATGGAT TTCCTCAATG AATTCTATGC TCATCCCCGG 
CTTCAGGACC ATTATGTTGT TCTCCTGTGC CCCACTGAGA TGGACCTGCC TGTGAGGAGA 
GTCCTGCAAA TTCCTCTCTG GTCCCAACGA GTCATCTATC TGCAGGGTTC TGCCCTCAAG 
GACCAGGATC TCATGAGAGC CAAAATTGAT GACGCAGAAG CCTGTTTTAT ACTCAGCAAT 
CGTTTCGAGC GAGACCGCAT CGCAGCTGAC CATCAGACCA TCCTCCGTGC CTGGGCCGTG 
AAGGACTTCG CACCAAACTG TCCCCTCTAC GTTCAGATAC TCAAACCGGA AAACAAGTTT 
CACATCAAAT TTGCAGATCA TGTGGTCTGT GAAGAAGAAT TCAAGTACGC CATGTTGGCT 
CTAAATTGCG TCTGCCCAGC GACTTCCACC CTGATAACTC TGCTCATCCA CACCTCTAGG 
GGGCAAGACA GTGCCAGTTC TCCGGATCAG TGGCAGAACA CCTACAGCCG CTGCTCGGCC 
AATGAAGTTT ATCACATCCG ACTGAGTGAA AGCAAATTCT TTGGAGAGTA TGAAGGGAAA 
AGCTTTACCT ATTCATCCTT CCACGCACAT AAAAAGTACG GGGTCTGTTT GATTGGTGTC 
CGTCGGGATA ACAAAACCAG CATCCTGCTG AATCCAGGGC CTTGCCATAT AATGTCCTCA 
ACAGATACCT GCTTCTACAT AAATATCTCA AAAGAAGAGA ACTCGGCATT CAGTTTGAAA 
GACAACGAAG AAGCCAAGAA ATCCGGGTAC CCAAAGTCTA CCTACCATGG GCTGACAAGG 
CTCCCTGTTC ACAGCATTAT AGCAAGCATG GGAACAGTGG CCATGGATCT CCAAGGCTCC 
AGAAGCCCAG AGAGTGTGGA TGGGAATATG CTGAGCCCAC CTGCTGATGG AACTCATACA 
AGGAAACACA GTATAGACGC AGTGCTAGAT GTTGTGGATG GCGACACCAG TACAAATTTC 
GACCTTTTGA GTGACCAATC AGAAGATGAG ACCAGCCAGT CAGACGAGGA GCTGGATTCC 
TCCATCAACC TGGTTAAGGG ATACCCCTCA AACACACCGT ACATTGGCAG CTCACCCACT 
CTGTGCCACT TGCTCCAGCA GAAGAATCCA TATTGTTGCC TGCGTCTTGA CAAGAGCTGC 
CAACATTACC CATATGAAGA TGCAAGGGCA TATGGCTTTA GAAATGCACT GGTGATAGTG 
TCTGCAGAGC ATGCTGGTAA CGGGCTGTAT AACTTTATTG TTCCACTAAG AGCTTACTAC 
AGAGGCAAGA AGGAGCTAAG TCCCATCATC CTGCTTCTGG AGAACCCGCC AGATGTTCAG 
TTCCTGGAAG CCATTTGCTG GTTCCCTCTG GTCTACTACA TGGTTGGATC CATCGATAAT 
CTAGATGACA TGCTGCGCTG TGGGGTCAGT TACGCGGCAC ACATGGTAGT TGTTGGAGAG 
GAAAGCTCTA TGATTGCAGA AGAAGACTAC ATGGCTGACG CGCGCACTAT TGTCAATGTG 
CAGACTTTAT TTAGGCTGTT CCCAGGGTTG AACATCATCA CAGAGCTAAC GCATCCTGCA 
AACATGAGGT TCATGCAGTT CAAAGCCAAG GATAAATACT CCGTTGTACT CTCCAAGCTG 
GAGAAGAGGG AACGGGAACG GGGCTCGAAC CTGGTCTTCA TGTTCAGACT GCCCTTTGCC 
GCTGGGAAGG TTTTCAGCGT CAGCATGTTA GACACCCTCC TATACCAGCT CCGAGTGACA 
GAAGAAGACC TGTGGATCCG TTGCTATGGG CGGCTGTATC AGAAGCTCTG TTCCACTACA 
GGCGATATTC CCATTGGGAT ATACCGTACT GAGACACACT TGCTGCCTGC CTCTGAGTCT 
CAGGTATCAA TCAGTGTTGA AGATTATGAA GATACAAGAG AGACTCGATG GGGAGACCCT 
AGCCAGGGGC GACCCCTTCA CCGGAACTCA ACCTCCAGTG ACCAATCAGA GCACCCCTTA 
CTGCGCAGAA AGAGCATGCA GTGGGCCAGA AGGCTAAGCC GCAAGGGAGC AAGGCAGCCT 
GGGAAATCGG CTTCGGAACG AATCAGCCAG CAGCGAATGA GTCTGTACCG GCGGTCGGAG 
CGGCAGGAAC TCAATGCACT GGTCAAGACC AGGATGAAGC ATTTGGGGCT CTCCACTGCT 
GAGTACAGTA TGAAACAGAT GGACATTATG GACACTGACA ACACTTTGTC CTATATCCTA 
ATAAACCCAT CTCCAGATAC CAAACTGGAA CTTAATGATG TTGTTTACCT CATCCGACCA 
GACCCTCTTA CTCTGGTGTC TATGAAGTCC CGTAAAGCTA GCGTCACACG CTCGGAAGGC 
ACGGTATACG ACATGAAGGA CAGCACCCAT CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012808575.1
CDS	2411..5806
Translation	MELEKDVQPLPPRYRFRDLLMGDWQQDDRVHVEFYVNENSFKERLKLFFIKNQRSSLRVRVFNCSLKLLSCLLYIIRVLLDDPRESRGGCWECTKQNYSAQQKFDWTPIIWVNRPLPLWGLQVLVALISFWETMLLTYLGYKGNIWEQLLRIPFILEMLNTLPFFVTIFWPPLRNVFIPVFLNCWLAKHALENMINDLHRAIQRTHSAMFNQVFILICTLICLMFTCICGIQHLERAGNNLTLFDSLYFCIVTFSTVGFGDVTPKIWPSQLLVVIMICVALIVLPIQFEQLVFLWMERQKSGGNYSRYRAQTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQDHYVVLLCPTEMDLPVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKIDDAEACFILSNRFERDRIAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHIKFADHVVCEEEFKYAMLALNCVCPATSTLITLLIHTSRGQDSASSPDQWQNTYSRCSANEVYHIRLSESKFFGEYEGKSFTYSSFHAHKKYGVCLIGVRRDNKTSILLNPGPCHIMSSTDTCFYINISKEENSAFSLKDNEEAKKSGYPKSTYHGLTRLPVHSIIASMGTVAMDLQGSRSPESVDGNMLSPPADGTHTRKHSIDAVLDVVDGDTSTNFDLLSDQSEDETSQSDEELDSSINLVKGYPSNTPYIGSSPTLCHLLQQKNPYCCLRLDKSCQHYPYEDARAYGFRNALVIVSAEHAGNGLYNFIVPLRAYYRGKKELSPIILLLENPPDVQFLEAICWFPLVYYMVGSIDNLDDMLRCGVSYAAHMVVVGEESSMIAEEDYMADARTIVNVQTLFRLFPGLNIITELTHPANMRFMQFKAKDKYSVVLSKLEKRERERGSNLVFMFRLPFAAGKVFSVSMLDTLLYQLRVTEEDLWIRCYGRLYQKLCSTTGDIPIGIYRTETHLLPASESQVSISVEDYEDTRETRWGDPSQGRPLHRNSTSSDQSEHPLLRRKSMQWARRLSRKGARQPGKSASERISQQRMSLYRRSERQELNALVKTRMKHLGLSTAEYSMKQMDIMDTDNTLSYILINPSPDTKLELNDVVYLIRPDPLTLVSMKSRKASVTRSEGTVYDMKDSTHL

Target ORF information:

RefSeq Version	XM_012953121.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis potassium channel, sodium activated subfamily T, member 1 (kcnt1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012953121.2

ORF Insert Sequence:

ATGGAGCTGG AGAAAGATGT GCAACCTTTG CCTCCACGCT ATCGATTTCG GGACTTGTTG 
ATGGGTGACT GGCAACAGGA TGACAGGGTC CATGTGGAAT TTTATGTCAA TGAAAACTCG 
TTTAAGGAAC GACTAAAGTT ATTTTTTATA AAGAATCAGA GATCAAGTTT GAGAGTCAGA 
GTCTTTAACT GTTCCTTAAA GTTACTGAGT TGCCTCCTCT ATATTATAAG GGTCCTTCTG 
GATGATCCGA GAGAGAGCAG GGGCGGATGC TGGGAATGTA CCAAACAGAA TTACTCTGCA 
CAACAGAAAT TTGACTGGAC CCCAATTATT TGGGTAAATA GACCTCTTCC CCTGTGGGGG 
CTTCAGGTAC TCGTGGCGCT AATTAGCTTC TGGGAAACAA TGCTTCTCAC CTACCTTGGC 
TACAAGGGAA ACATATGGGA GCAGCTCCTC CGGATCCCCT TCATTCTAGA GATGCTGAAC 
ACCCTGCCTT TCTTTGTCAC GATTTTCTGG CCTCCGTTGA GGAATGTCTT CATTCCTGTT 
TTCCTCAATT GCTGGTTGGC TAAACATGCG CTGGAGAACA TGATAAATGA CTTGCACCGA 
GCCATTCAGA GGACACACTC GGCCATGTTT AACCAGGTCT TTATTCTGAT ATGCACCCTC 
ATCTGCCTCA TGTTCACTTG CATCTGTGGG ATTCAGCATC TGGAGAGAGC GGGCAATAAC 
CTCACCCTCT TTGACTCCCT GTATTTCTGC ATTGTCACTT TCTCCACGGT GGGCTTCGGA 
GACGTCACCC CAAAGATCTG GCCGTCACAG CTCCTGGTTG TCATTATGAT CTGTGTGGCA 
CTCATTGTGC TGCCCATCCA GTTTGAGCAG CTCGTCTTCC TATGGATGGA AAGGCAGAAA 
TCTGGCGGGA ATTACAGCCG ATACCGAGCG CAGACGGAGA AGCACGTGGT CCTGTGCGTC 
AGTTCTTTGA AGATTGACCT GCTTATGGAT TTCCTCAATG AATTCTATGC TCATCCCCGG 
CTTCAGGACC ATTATGTTGT TCTCCTGTGC CCCACTGAGA TGGACCTGCC TGTGAGGAGA 
GTCCTGCAAA TTCCTCTCTG GTCCCAACGA GTCATCTATC TGCAGGGTTC TGCCCTCAAG 
GACCAGGATC TCATGAGAGC CAAAATTGAT GACGCAGAAG CCTGTTTTAT ACTCAGCAAT 
CGTTTCGAGC GAGACCGCAT CGCAGCTGAC CATCAGACCA TCCTCCGTGC CTGGGCCGTG 
AAGGACTTCG CACCAAACTG TCCCCTCTAC GTTCAGATAC TCAAACCGGA AAACAAGTTT 
CACATCAAAT TTGCAGATCA TGTGGTCTGT GAAGAAGAAT TCAAGTACGC CATGTTGGCT 
CTAAATTGCG TCTGCCCAGC GACTTCCACC CTGATAACTC TGCTCATCCA CACCTCTAGG 
GGGCAAGACA GTGCCAGTTC TCCGGATCAG TGGCAGAACA CCTACAGCCG CTGCTCGGCC 
AATGAAGTTT ATCACATCCG ACTGAGTGAA AGCAAATTCT TTGGAGAGTA TGAAGGGAAA 
AGCTTTACCT ATTCATCCTT CCACGCACAT AAAAAGTACG GGGTCTGTTT GATTGGTGTC 
CGTCGGGATA ACAAAACCAG CATCCTGCTG AATCCAGGGC CTTGCCATAT AATGTCCTCA 
ACAGATACCT GCTTCTACAT AAATATCTCA AAAGAAGAGA ACTCGGCATT CAGTTTGAAA 
GACAACGAAG AAGCCAAGAA ATCCGGGTAC CCAAAGTCTA CCTACCATGG GCTGACAAGG 
CTCCCTGTTC ACAGCATTAT AGCAAGCATG GGAACAGTGG CCATGGATCT CCAAGGCTCC 
AGAAGCCCAG AGAGTGTGGA TGGGAATATG CTGAGCCCAC CTGCTGATGG AACTCATACA 
AGGAAACACA GTATAGACGC AGTGCTAGAT GTTGTGGATG GCGACACCAG TACAAATTTC 
GACCTTTTGA GTGACCAATC AGAAGATGAG ACCAGCCAGT CAGACGAGGA GCTGGATTCC 
TCCATCAACC TGGTTAAGGG ATACCCCTCA AACACACCGT ACATTGGCAG CTCACCCACT 
CTGTGCCACT TGCTCCAGCA GAAGAATCCA TATTGTTGCC TGCGTCTTGA CAAGAGCTGC 
CAACATTACC CATATGAAGA TGCAAGGGCA TATGGCTTTA GAAATGCACT GGTGATAGTG 
TCTGCAGAGC ATGCTGGTAA CGGGCTGTAT AACTTTATTG TTCCACTAAG AGCTTACTAC 
AGAGGCAAGA AGGAGCTAAG TCCCATCATC CTGCTTCTGG AGAACCCGCC AGATGTTCAG 
TTCCTGGAAG CCATTTGCTG GTTCCCTCTG GTCTACTACA TGGTTGGATC CATCGATAAT 
CTAGATGACA TGCTGCGCTG TGGGGTCAGT TACGCGGCAC ACATGGTAGT TGTTGGAGAG 
GAAAGCTCTA TGATTGCAGA AGAAGACTAC ATGGCTGACG CGCGCACTAT TGTCAATGTG 
CAGACTTTAT TTAGGCTGTT CCCAGGGTTG AACATCATCA CAGAGCTAAC GCATCCTGCA 
AACATGAGGT TCATGCAGTT CAAAGCCAAG GATAAATACT CCGTTGTACT CTCCAAGCTG 
GAGAAGAGGG AACGGGAACG GGGCTCGAAC CTGGTCTTCA TGTTCAGACT GCCCTTTGCC 
GCTGGGAAGG TTTTCAGCGT CAGCATGTTA GACACCCTCC TATACCAGCT CCGAGTGACA 
GAAGAAGACC TGTGGATCCG TTGCTATGGG CGGCTGTATC AGAAGCTCTG TTCCACTACA 
GGCGATATTC CCATTGGGAT ATACCGTACT GAGACACACT TGCTGCCTGC CTCTGAGTCT 
CAGGTATCAA TCAGTGTTGA AGATTATGAA GATACAAGAG AGACTCGATG GGGAGACCCT 
AGCCAGGGGC GACCCCTTCA CCGGAACTCA ACCTCCAGTG ACCAATCAGA GCACCCCTTA 
CTGCGCAGAA AGAGCATGCA GTGGGCCAGA AGGCTAAGCC GCAAGGGAGC AAGGCAGCCT 
GGGAAATCGG CTTCGGAACG AATCAGCCAG CAGCGAATGA GTCTGTACCG GCGGTCGGAG 
CGGCAGGAAC TCAATGCACT GGTCAAGACC AGGATGAAGC ATTTGGGGCT CTCCACTGCT 
GAGTACAGTA TGAAACAGAT GGACATTATG GACACTGACA ACACTTTGTC CTATATCCTA 
ATAAACCCAT CTCCAGATAC CAAACTGGAA CTTAATGATG TTGTTTACCT CATCCGACCA 
GACCCTCTTA CTCTGGTGTC TATGAAGTCC CGTAAAGCTA GCGTCACACG CTCGGAAGGC 
ACGGTATACG ACATGAAGGA CAGCACCCAT CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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kcnt1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

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Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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