brwd3 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	brwd3
Entrez Gene ID	733720
Full Name	bromodomain and WD repeat domain containing 3
General protein information	Preferred Names bromodomain and WD repeat domain containing 3 Names bromodomain and WD repeat-containing protein 3 novel WD repeat protein
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018096621.1	XP_017952110.1	bromodomain and WD repeat-containing protein 3 isoform X3
XM_018096621.2	XP_017952110.2	bromodomain and WD repeat-containing protein 3 isoform X3
XM_004916894.3	XP_004916951.1	bromodomain and WD repeat-containing protein 3 isoform X2
XM_004916894.4	XP_004916951.2	bromodomain and WD repeat-containing protein 3 isoform X2
XM_002940436.4	XP_002940482.2	bromodomain and WD repeat-containing protein 3 isoform X1
XM_002940436.5	XP_002940482.3	bromodomain and WD repeat-containing protein 3 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	BRWD3	NP_694984.4
Macaca mulatta (Rhesus monkey)	BRWD3	XP_002806340.1
Canis lupus familiaris (dog)	BRWD3	XP_005641586.1
Mus musculus (house mouse)	Brwd3	NP_001074946.1
Rattus norvegicus (Norway rat)	Brwd3	XP_006257245.1
Gallus gallus (chicken)	BRWD3	XP_003641127.2
Bos taurus (cattle)	BRWD3	XP_002699983.1
Xenopus tropicalis (tropical clawed frog)	brwd3	XP_002940482.2
Pan troglodytes (chimpanzee)	BRWD3	XP_003954075.1

Gene Ontology

The following brwd3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the brwd3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa14411	XM_002940436.4 Latest version!	Xenopus tropicalis bromodomain and WD repeat domain containing 3 (brwd3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa14412	XM_004916894.3 Latest version!	Xenopus tropicalis bromodomain and WD repeat domain containing 3 (brwd3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa46289	XM_018096621.1 Latest version!	Xenopus tropicalis bromodomain and WD repeat domain containing 3 (brwd3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa64563	XM_002940436.5 Latest version!	Xenopus tropicalis bromodomain and WD repeat domain containing 3 (brwd3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa64564	XM_004916894.4 Latest version!	Xenopus tropicalis bromodomain and WD repeat domain containing 3 (brwd3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa64565	XM_018096621.2 Latest version!	Xenopus tropicalis bromodomain and WD repeat domain containing 3 (brwd3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

brwd3 ( XM_002940436.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002940482.2 Xenopus tropicalis bromodomain and WD repeat domain containing 3 (brwd3), transcript variant X1, mRNA.

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CloneID	OXa14411
Clone ID Related Accession (Same CDS sequence)	XM_002940436.4
Accession Version	XM_002940436.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5331bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	bromodomain and WD repeat-containing protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002940436.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCCG AGCTTTACTT CCTGATCGCC CGCTTCCTAC AGTCTGGCCC CTGCCGGAGA 
TCCGCACAGG TTCTGGCGGA GGAACTGGAG GAGTACCGGC TCATCCCACC GCGCTTAGAC 
TGGGAAGGGA AGGAACACCG CAGAACTTAT CAGGATTTGG TGGCAGCCAA TAAACACATC 
CCATCAGACT ACCTGCTTAA AATCTGCGAG CGTTTTGGAC CTATCCTAGA TCAAGAAATC 
CCGCAGAGTG TTCCTGGGTT GCATAGTCTG TTGGGACTTG GAAAACAGTC ACTGCTCAGA 
AGTAAAAAAG ACTGCAAGAA TACACCATGG AAAGGGACAG CTTTTGCAGC TTTGCACAGA 
GGGAGACCAC CAGAACTTCC AGTCAACTAT GGCAAGCCCC TCAACGTTGT AAATATCACT 
TGCGCCAGAT ATCAGACTGG ATATGCACAT TACCATCGCA TATTTCCATC TTCAACTTAT 
CAGCAGATGA AAATGCACAA GAGGATTTTG GGCCATCTTT CATCTGTATA CTGTGTGGCT 
TTTGATCGCA CTGGAAGACG TATTTTCACT GGTTCAGATG ACTGTCTAGT CAAAATATGG 
GCAAGTGATG ATGGACGTCT TCTTTCCACA CTACGTGGCC ATTCAGCTGA AATCTCTGAT 
CTGGCTGTGA ATCATGAGAA TACATTGATT GCTGCTGGCA GCTGTGATAA GGTGGTGCGT 
GTGTGGTGCC TTCGTACATG TGCACCAATA GCTGTGCTGC AGGGTCATAC AGCTTCTATC 
ACTTCCATAC AGTTTTGCCC AGGAACAAAA GAAAAAACAA GATACCTCAC ATCTACTGGT 
GCTGATGGGA CAATCTGCTT CTGGGAGTGG CATGCAGATA CCATGACATT TAATGATCGC 
CCTGTGAAAT TTGCAGAGAG GTCCAGGCCT GGGGTTCAGA TCTCCTGCTC ATCCTTTAGT 
TCAGGTGGTA TGTTTATAGC CACAGGAAGT ACTGATCATG TGATAAGAAT ATACTACCTG 
GGATCTGGTT CACCAGAGAA GATAGCTGAA TTGGAAGCAC ATTTGGACAA AGTGGTCGCT 
ATTCAGTTCT GCAACAATTC AGATCGATTG TGCTTTGTCA GTGGAAGCAG AGATGGCACT 
GCTCGGATTT GGCAATATCA GCAGCAAGAG TGGAAAATGA CTGTTCTGGA TATGACTGCC 
AAGATGGCTG GAAGCAGTGT TATAAATACA GAAGACAAAG TGACCAAGCT TAAAGTCACT 
ATGGTAGCAT GGGACCGATC TGATTCTTCG GTTATTACTG CTGTAAACAA TTTCTTTCTG 
AAAGTTTGGA ACTCTCATAC TGGCGAACTT CTTCATATAT TGTCTGGCCA TGATGATGAA 
GTTTTTGTTT TGGAACCTCA TCCTTTTGAT CCAAGGATTT TAATCTCTGC TGGGCATGAT 
GGAAACATAT TTGTGTGGGA CCTCGAAACA GGAACCAAAA TTCGTAATTA CTTTAACATG 
ATTGAAGGTC AGGGTCATGG AGCCGTGTTT GACTGTAAGT TCTCGCCTGA TGGTCAAAAC 
TTTGCCTGTA CAGATTCTCA TGGACATCTT TTACTCTTTG GTTTTGGTTG CAGTAAACCA 
TATGAAAAGA TTCCAGAACA GATGTTCTTC CATACAGACT TTAGACCTCT AATAAGAGAC 
TCCAACAGCT ATGTTTTGGA TGAACAAACT CAACAAGCCC CACACCTAAT GCCCCCTCCA 
TTCCTAGTAG ACGTTGATGG CAACCCACAT CCTACCCGTT TTCAGCGCTT GGTGCCAGGA 
CGTGAGAATT GTAAAGATGA GCAGCTCATA CCTCAGCTGG GATACATGTC AAATGGCAAT 
GGCGATGTGG TGGAGCAGGT CATTGGCCAG CAAACAGGAA ACCAGGAGGA AAGTATTCTT 
GATGGGATAA TCCGAGAACT CCAGAGGGAA CAGGATGAGA GGCATACAAA TGAAGCCGAG 
GATGTGGATA CACCCAGATA TAACAGAACA GACTCGAGAA ATGGAGTATT AAGAAGTATT 
AGCTTGGATT TATCATCTCC ACCTAATGTT GGCCTTCGCC GGAGTGGCCA AATTGAGGGT 
GTTCGCCAAA TGCATAGTAA TGCACCTCGG AGTCAAATTG CAACAGAAAG AGACCTAATG 
GCCTGGAGCA GAAGAGTGGT AGTAAATGAA CTGCATCCTG GTGTTATCAG GGTCCAGGAA 
GAATGCAGAA TTTCTAAAGG CGAAATGGAA ATCAGTTTGT ACAACACAGA GAAAAAGAGG 
AAACCAACTA ACCCTGCTCA GAGGAATGAT TATCAAGCTT TTGGTGGGCG ATCACTTAGA 
CGAATACAGC GGAAGCGCCA GCATGCCTAC CAGACACGTT CAACCATTGA ACATGATGCA 
AGAGCTAAAG TTCCAAGTAC AAATGGGGAA GATTCGCAGA GTTCCCCCGA GGAGGAGGAG 
TCCCAACATT CAACAGAGGC TTCAGGAGAT GAAGCTGCAG CTCAGTGGCA ATCAGAAACC 
TCTTCCAGTA ATTCATCTAG TGAATATTCC GACTGGACTG CAGATGCAGG AATCAATCTC 
CAGCCTCCCA AAAGACAGAC ACGGCAAACA ACAAGGAAAA TGTGCAGCAG TTCAGAGGAT 
GAGAACGCTA AAGAGGAAGA GAAGCCGAGG AAACACAAAC TGACCAGACG GAAGAAACAA 
AGTGAGCGTC TTTCTATGGA AGGAGAGCCA AGTGAAGAAT GGATTCCCCC CCAGTGGATC 
TTGGACACCA TCCCACGTCG TTCTCCTTTT GTTCCCCAGA TGGGAGATGA GGTTATTTAT 
TTCAGACAAG GCCATGAAGC TTATGTACGA GCAGTAAGGA AGGCTAAAAT ATATAATATT 
AATCCACAAA AGGAGCCATG GAACAAAACG GATCTCAGGG ATCAAGAATT TGCTAAGATT 
GTAGGAATTA AGTATGAGGT GGGGCCGCCT ACATTATGCT GCTTAAAACT TGCCTTACTG 
GATCCTACTT CAGGCAAAAT AACTGCAGAC TCTTTCTCTG TTAAATACCA TGACATGCCT 
GATGTTATTG ACTTCCTTGT CCTTTACCAG TTTTATAAGG AGGCGAAAGA GAGAAACTGG 
CAGATAGGAG ACCGGTTTCG AAGCATTATT GATGATGCAT GGTGGTTTGG CACAGTGGAA 
AGTCAGCAGC CATTCCAGAC TGAGTATCCT GACAGTTTAT TCCAGTGCTA CAGTGTACAC 
TGGGATAATA ATGAAAGAGA AAACATGAGT CCTTGGGACA TGGAACCAAT TCCAGATGGC 
ACAGCTTTGC CTGATGAAAT GGGTGCTGGT GTTCCCGTTA CTTCCGATGA ACTGAAGTCT 
TTGCAGTATA AACCGGAGGA GGGAGAGTGG GGATTGTACT CAAGAGATGA GGTTTGCGAA 
AGAGTTATCC AAGGAATTGA TCAGCTACTT ACTCTTGATT TTGCCAGCCC ATTCAGTGCT 
CCTGTGGATC TCAGTGCCTA CCCCTTATAC TGCACTGTGG TAGCATACCC AACAGATCTA 
AGCACCACAA GGAAGAGACT GGAGAGCCAC TACTATAGGA GGCTTTCTGC CCTAATATGG 
GAGGTCCGCT ATATTGAACA CAATGCAAGA ACATTCAATG AGCCTGACAC TCCAATTGTT 
AGAACTGCCA AAATTGTGAC AGATGTGCTG TTGCGTTATA TTGGGGATGA GAATTGTAAT 
GATATATTAG AGATCTACAA TCAAGTAAAG ACTGAAGATA CTGGCAATAC AGAAGATGAG 
GAGGGTGGAG CAGTTGATAT GGAATCAGAT GCCCCAGGGA CATCATCTGG TCGGGCTGTT 
ACCCGTCGCA CTAAAAGAGA TCTACTTCGT TCCAATCCTG ATTCATGGAA AGATAAATGC 
AAAGAACTAC TAGATTTAAT ATATGAGCGG GAAGATTCTG AACCCTTTAG GCAACCAGTC 
AATCAAGTCT CATATCCTGA TTATCAGGAT ATTATTGAAA CACCAATGGA TTTTAGCACC 
GTGAAAACAA CGTTAGAAGC TGGAAATTAT AGTGACCCGT TGGAATTTGC TGCAGATGTT 
CGACTAATCT TCAGTAACTC CAAAGCATAC ACTCCAAACA AAAAATCTAG AATCTACAGT 
ATGACTCTTC GACTCTCTGC ACTATGTGAA ACCTACATAA AGGATATCAT CTCTGACTAC 
AAACTAGCCA TGCAACTCCA ACAGAGAACA AGGCGGAAAT ACAGAAGGCG AATTAGGAGC 
AGCAGCAGCA GCAGCAGCAG CAGCAGTAGT AATAGAAGAA GAAGTAGTAG TAGTAGTAGT 
AGTTCTAGTT CAGCATCCAG CAGAGCATCC AGCCCAGACA CAAAACCTCG GAAAATGAAG 
CTTCAACCGA AACTTAATCA GAACCCTCCA CATCCTCTTC CTAGGAGCAG CTCTTCATCC 
ACTTCTCAAA TGTCTGATAC TGATGTAGAG CCTGATGGAA AGCCTTTTTC TGAAGATATG 
GATCAAGAGC CATCTTCATC TGGATGCAGC AAACCTGAAA CACACAAAGA ACAGGAGAAT 
GTGACCAAGA GCAAAAATTC TCCTGTGAAA GAGGAACATA CAAATGGCCC TCTAACAAAT 
GGCGATGGAG GAAACACACC AAAAACCAAG AGAAAGTTGC TAAGTTCTTC TGAGGATGAA 
AGTCCAGAAG ATGGAGAGCA AATGGAAGAA AAGCCTGATA AAAGTCCAGC ATGTTCTCGT 
GAAAGAGACT CTGAATCAAG TTTAAGTTCG AAGAGTGAGT CAGAATCAGA GAGCAGTTCT 
GATTCTGAAT CCATTAAGTC AGATCAGGAT TATATTGATG GCGACCATCA TTATAGCAAA 
GAGCCCCGTA CAAAGTTTAA GAGAAAATTT TGTAAATCTG GAGAGGCTAA AACGAGAGGA 
AGGGGTCGAG GAAGAGGCAG AGGAAGAGGA CGTGCAAAAG GTTATAATCG TGGTGTGGAG 
GAAGAGTTAA AAAAAGGTAG GGGCCGGGGC AGAGGAAGAG GAAGAGGAGC ATGGAAAAAA 
GAACCTTCAA GGACACCGGC ATTAAAACGT GCGCAATTGA CAGATGATGA AGATGATGAC 
AATGTGGATG ATGAAGAAAG TGATTGTGTA GAGCAACGAA GAAAAAAACC TAGGATAAAC 
ACCAGGAACC AGGGCAGAAG AACTGTGTTA TATAATGATG AGTCTGATAA TGAGCATGAT 
GGTGCGGGGC AATATGATGA TCCCTTAAAC CTGGGAACAT CCAGGTCTGG AAGAGTGAGA 
AAAATGACGG AAAAGGCCAG AGTGAGTCAT CTGATGGGAT GGAATGGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002940482.2
CDS	1169..6499
Translation	MEAELYFLIARFLQSGPCRRSAQVLAEELEEYRLIPPRLDWEGKEHRRTYQDLVAANKHIPSDYLLKICERFGPILDQEIPQSVPGLHSLLGLGKQSLLRSKKDCKNTPWKGTAFAALHRGRPPELPVNYGKPLNVVNITCARYQTGYAHYHRIFPSSTYQQMKMHKRILGHLSSVYCVAFDRTGRRIFTGSDDCLVKIWASDDGRLLSTLRGHSAEISDLAVNHENTLIAAGSCDKVVRVWCLRTCAPIAVLQGHTASITSIQFCPGTKEKTRYLTSTGADGTICFWEWHADTMTFNDRPVKFAERSRPGVQISCSSFSSGGMFIATGSTDHVIRIYYLGSGSPEKIAELEAHLDKVVAIQFCNNSDRLCFVSGSRDGTARIWQYQQQEWKMTVLDMTAKMAGSSVINTEDKVTKLKVTMVAWDRSDSSVITAVNNFFLKVWNSHTGELLHILSGHDDEVFVLEPHPFDPRILISAGHDGNIFVWDLETGTKIRNYFNMIEGQGHGAVFDCKFSPDGQNFACTDSHGHLLLFGFGCSKPYEKIPEQMFFHTDFRPLIRDSNSYVLDEQTQQAPHLMPPPFLVDVDGNPHPTRFQRLVPGRENCKDEQLIPQLGYMSNGNGDVVEQVIGQQTGNQEESILDGIIRELQREQDERHTNEAEDVDTPRYNRTDSRNGVLRSISLDLSSPPNVGLRRSGQIEGVRQMHSNAPRSQIATERDLMAWSRRVVVNELHPGVIRVQEECRISKGEMEISLYNTEKKRKPTNPAQRNDYQAFGGRSLRRIQRKRQHAYQTRSTIEHDARAKVPSTNGEDSQSSPEEEESQHSTEASGDEAAAQWQSETSSSNSSSEYSDWTADAGINLQPPKRQTRQTTRKMCSSSEDENAKEEEKPRKHKLTRRKKQSERLSMEGEPSEEWIPPQWILDTIPRRSPFVPQMGDEVIYFRQGHEAYVRAVRKAKIYNINPQKEPWNKTDLRDQEFAKIVGIKYEVGPPTLCCLKLALLDPTSGKITADSFSVKYHDMPDVIDFLVLYQFYKEAKERNWQIGDRFRSIIDDAWWFGTVESQQPFQTEYPDSLFQCYSVHWDNNERENMSPWDMEPIPDGTALPDEMGAGVPVTSDELKSLQYKPEEGEWGLYSRDEVCERVIQGIDQLLTLDFASPFSAPVDLSAYPLYCTVVAYPTDLSTTRKRLESHYYRRLSALIWEVRYIEHNARTFNEPDTPIVRTAKIVTDVLLRYIGDENCNDILEIYNQVKTEDTGNTEDEEGGAVDMESDAPGTSSGRAVTRRTKRDLLRSNPDSWKDKCKELLDLIYEREDSEPFRQPVNQVSYPDYQDIIETPMDFSTVKTTLEAGNYSDPLEFAADVRLIFSNSKAYTPNKKSRIYSMTLRLSALCETYIKDIISDYKLAMQLQQRTRRKYRRRIRSSSSSSSSSSSNRRRSSSSSSSSSSASSRASSPDTKPRKMKLQPKLNQNPPHPLPRSSSSSTSQMSDTDVEPDGKPFSEDMDQEPSSSGCSKPETHKEQENVTKSKNSPVKEEHTNGPLTNGDGGNTPKTKRKLLSSSEDESPEDGEQMEEKPDKSPACSRERDSESSLSSKSESESESSSDSESIKSDQDYIDGDHHYSKEPRTKFKRKFCKSGEAKTRGRGRGRGRGRGRAKGYNRGVEEELKKGRGRGRGRGRGAWKKEPSRTPALKRAQLTDDEDDDNVDDEESDCVEQRRKKPRINTRNQGRRTVLYNDESDNEHDGAGQYDDPLNLGTSRSGRVRKMTEKARVSHLMGWNG

Target ORF information:

RefSeq Version	XM_002940436.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis bromodomain and WD repeat domain containing 3 (brwd3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002940436.4

ORF Insert Sequence:

ATGGAAGCCG AGCTTTACTT CCTGATCGCC CGCTTCCTAC AGTCTGGCCC CTGCCGGAGA 
TCCGCACAGG TTCTGGCGGA GGAACTGGAG GAGTACCGGC TCATCCCACC GCGCTTAGAC 
TGGGAAGGGA AGGAACACCG CAGAACTTAT CAGGATTTGG TGGCAGCCAA TAAACACATC 
CCATCAGACT ACCTGCTTAA AATCTGCGAG CGTTTTGGAC CTATCCTAGA TCAAGAAATC 
CCGCAGAGTG TTCCTGGGTT GCATAGTCTG TTGGGACTTG GAAAACAGTC ACTGCTCAGA 
AGTAAAAAAG ACTGCAAGAA TACACCATGG AAAGGGACAG CTTTTGCAGC TTTGCACAGA 
GGGAGACCAC CAGAACTTCC AGTCAACTAT GGCAAGCCCC TCAACGTTGT AAATATCACT 
TGCGCCAGAT ATCAGACTGG ATATGCACAT TACCATCGCA TATTTCCATC TTCAACTTAT 
CAGCAGATGA AAATGCACAA GAGGATTTTG GGCCATCTTT CATCTGTATA CTGTGTGGCT 
TTTGATCGCA CTGGAAGACG TATTTTCACT GGTTCAGATG ACTGTCTAGT CAAAATATGG 
GCAAGTGATG ATGGACGTCT TCTTTCCACA CTACGTGGCC ATTCAGCTGA AATCTCTGAT 
CTGGCTGTGA ATCATGAGAA TACATTGATT GCTGCTGGCA GCTGTGATAA GGTGGTGCGT 
GTGTGGTGCC TTCGTACATG TGCACCAATA GCTGTGCTGC AGGGTCATAC AGCTTCTATC 
ACTTCCATAC AGTTTTGCCC AGGAACAAAA GAAAAAACAA GATACCTCAC ATCTACTGGT 
GCTGATGGGA CAATCTGCTT CTGGGAGTGG CATGCAGATA CCATGACATT TAATGATCGC 
CCTGTGAAAT TTGCAGAGAG GTCCAGGCCT GGGGTTCAGA TCTCCTGCTC ATCCTTTAGT 
TCAGGTGGTA TGTTTATAGC CACAGGAAGT ACTGATCATG TGATAAGAAT ATACTACCTG 
GGATCTGGTT CACCAGAGAA GATAGCTGAA TTGGAAGCAC ATTTGGACAA AGTGGTCGCT 
ATTCAGTTCT GCAACAATTC AGATCGATTG TGCTTTGTCA GTGGAAGCAG AGATGGCACT 
GCTCGGATTT GGCAATATCA GCAGCAAGAG TGGAAAATGA CTGTTCTGGA TATGACTGCC 
AAGATGGCTG GAAGCAGTGT TATAAATACA GAAGACAAAG TGACCAAGCT TAAAGTCACT 
ATGGTAGCAT GGGACCGATC TGATTCTTCG GTTATTACTG CTGTAAACAA TTTCTTTCTG 
AAAGTTTGGA ACTCTCATAC TGGCGAACTT CTTCATATAT TGTCTGGCCA TGATGATGAA 
GTTTTTGTTT TGGAACCTCA TCCTTTTGAT CCAAGGATTT TAATCTCTGC TGGGCATGAT 
GGAAACATAT TTGTGTGGGA CCTCGAAACA GGAACCAAAA TTCGTAATTA CTTTAACATG 
ATTGAAGGTC AGGGTCATGG AGCCGTGTTT GACTGTAAGT TCTCGCCTGA TGGTCAAAAC 
TTTGCCTGTA CAGATTCTCA TGGACATCTT TTACTCTTTG GTTTTGGTTG CAGTAAACCA 
TATGAAAAGA TTCCAGAACA GATGTTCTTC CATACAGACT TTAGACCTCT AATAAGAGAC 
TCCAACAGCT ATGTTTTGGA TGAACAAACT CAACAAGCCC CACACCTAAT GCCCCCTCCA 
TTCCTAGTAG ACGTTGATGG CAACCCACAT CCTACCCGTT TTCAGCGCTT GGTGCCAGGA 
CGTGAGAATT GTAAAGATGA GCAGCTCATA CCTCAGCTGG GATACATGTC AAATGGCAAT 
GGCGATGTGG TGGAGCAGGT CATTGGCCAG CAAACAGGAA ACCAGGAGGA AAGTATTCTT 
GATGGGATAA TCCGAGAACT CCAGAGGGAA CAGGATGAGA GGCATACAAA TGAAGCCGAG 
GATGTGGATA CACCCAGATA TAACAGAACA GACTCGAGAA ATGGAGTATT AAGAAGTATT 
AGCTTGGATT TATCATCTCC ACCTAATGTT GGCCTTCGCC GGAGTGGCCA AATTGAGGGT 
GTTCGCCAAA TGCATAGTAA TGCACCTCGG AGTCAAATTG CAACAGAAAG AGACCTAATG 
GCCTGGAGCA GAAGAGTGGT AGTAAATGAA CTGCATCCTG GTGTTATCAG GGTCCAGGAA 
GAATGCAGAA TTTCTAAAGG CGAAATGGAA ATCAGTTTGT ACAACACAGA GAAAAAGAGG 
AAACCAACTA ACCCTGCTCA GAGGAATGAT TATCAAGCTT TTGGTGGGCG ATCACTTAGA 
CGAATACAGC GGAAGCGCCA GCATGCCTAC CAGACACGTT CAACCATTGA ACATGATGCA 
AGAGCTAAAG TTCCAAGTAC AAATGGGGAA GATTCGCAGA GTTCCCCCGA GGAGGAGGAG 
TCCCAACATT CAACAGAGGC TTCAGGAGAT GAAGCTGCAG CTCAGTGGCA ATCAGAAACC 
TCTTCCAGTA ATTCATCTAG TGAATATTCC GACTGGACTG CAGATGCAGG AATCAATCTC 
CAGCCTCCCA AAAGACAGAC ACGGCAAACA ACAAGGAAAA TGTGCAGCAG TTCAGAGGAT 
GAGAACGCTA AAGAGGAAGA GAAGCCGAGG AAACACAAAC TGACCAGACG GAAGAAACAA 
AGTGAGCGTC TTTCTATGGA AGGAGAGCCA AGTGAAGAAT GGATTCCCCC CCAGTGGATC 
TTGGACACCA TCCCACGTCG TTCTCCTTTT GTTCCCCAGA TGGGAGATGA GGTTATTTAT 
TTCAGACAAG GCCATGAAGC TTATGTACGA GCAGTAAGGA AGGCTAAAAT ATATAATATT 
AATCCACAAA AGGAGCCATG GAACAAAACG GATCTCAGGG ATCAAGAATT TGCTAAGATT 
GTAGGAATTA AGTATGAGGT GGGGCCGCCT ACATTATGCT GCTTAAAACT TGCCTTACTG 
GATCCTACTT CAGGCAAAAT AACTGCAGAC TCTTTCTCTG TTAAATACCA TGACATGCCT 
GATGTTATTG ACTTCCTTGT CCTTTACCAG TTTTATAAGG AGGCGAAAGA GAGAAACTGG 
CAGATAGGAG ACCGGTTTCG AAGCATTATT GATGATGCAT GGTGGTTTGG CACAGTGGAA 
AGTCAGCAGC CATTCCAGAC TGAGTATCCT GACAGTTTAT TCCAGTGCTA CAGTGTACAC 
TGGGATAATA ATGAAAGAGA AAACATGAGT CCTTGGGACA TGGAACCAAT TCCAGATGGC 
ACAGCTTTGC CTGATGAAAT GGGTGCTGGT GTTCCCGTTA CTTCCGATGA ACTGAAGTCT 
TTGCAGTATA AACCGGAGGA GGGAGAGTGG GGATTGTACT CAAGAGATGA GGTTTGCGAA 
AGAGTTATCC AAGGAATTGA TCAGCTACTT ACTCTTGATT TTGCCAGCCC ATTCAGTGCT 
CCTGTGGATC TCAGTGCCTA CCCCTTATAC TGCACTGTGG TAGCATACCC AACAGATCTA 
AGCACCACAA GGAAGAGACT GGAGAGCCAC TACTATAGGA GGCTTTCTGC CCTAATATGG 
GAGGTCCGCT ATATTGAACA CAATGCAAGA ACATTCAATG AGCCTGACAC TCCAATTGTT 
AGAACTGCCA AAATTGTGAC AGATGTGCTG TTGCGTTATA TTGGGGATGA GAATTGTAAT 
GATATATTAG AGATCTACAA TCAAGTAAAG ACTGAAGATA CTGGCAATAC AGAAGATGAG 
GAGGGTGGAG CAGTTGATAT GGAATCAGAT GCCCCAGGGA CATCATCTGG TCGGGCTGTT 
ACCCGTCGCA CTAAAAGAGA TCTACTTCGT TCCAATCCTG ATTCATGGAA AGATAAATGC 
AAAGAACTAC TAGATTTAAT ATATGAGCGG GAAGATTCTG AACCCTTTAG GCAACCAGTC 
AATCAAGTCT CATATCCTGA TTATCAGGAT ATTATTGAAA CACCAATGGA TTTTAGCACC 
GTGAAAACAA CGTTAGAAGC TGGAAATTAT AGTGACCCGT TGGAATTTGC TGCAGATGTT 
CGACTAATCT TCAGTAACTC CAAAGCATAC ACTCCAAACA AAAAATCTAG AATCTACAGT 
ATGACTCTTC GACTCTCTGC ACTATGTGAA ACCTACATAA AGGATATCAT CTCTGACTAC 
AAACTAGCCA TGCAACTCCA ACAGAGAACA AGGCGGAAAT ACAGAAGGCG AATTAGGAGC 
AGCAGCAGCA GCAGCAGCAG CAGCAGTAGT AATAGAAGAA GAAGTAGTAG TAGTAGTAGT 
AGTTCTAGTT CAGCATCCAG CAGAGCATCC AGCCCAGACA CAAAACCTCG GAAAATGAAG 
CTTCAACCGA AACTTAATCA GAACCCTCCA CATCCTCTTC CTAGGAGCAG CTCTTCATCC 
ACTTCTCAAA TGTCTGATAC TGATGTAGAG CCTGATGGAA AGCCTTTTTC TGAAGATATG 
GATCAAGAGC CATCTTCATC TGGATGCAGC AAACCTGAAA CACACAAAGA ACAGGAGAAT 
GTGACCAAGA GCAAAAATTC TCCTGTGAAA GAGGAACATA CAAATGGCCC TCTAACAAAT 
GGCGATGGAG GAAACACACC AAAAACCAAG AGAAAGTTGC TAAGTTCTTC TGAGGATGAA 
AGTCCAGAAG ATGGAGAGCA AATGGAAGAA AAGCCTGATA AAAGTCCAGC ATGTTCTCGT 
GAAAGAGACT CTGAATCAAG TTTAAGTTCG AAGAGTGAGT CAGAATCAGA GAGCAGTTCT 
GATTCTGAAT CCATTAAGTC AGATCAGGAT TATATTGATG GCGACCATCA TTATAGCAAA 
GAGCCCCGTA CAAAGTTTAA GAGAAAATTT TGTAAATCTG GAGAGGCTAA AACGAGAGGA 
AGGGGTCGAG GAAGAGGCAG AGGAAGAGGA CGTGCAAAAG GTTATAATCG TGGTGTGGAG 
GAAGAGTTAA AAAAAGGTAG GGGCCGGGGC AGAGGAAGAG GAAGAGGAGC ATGGAAAAAA 
GAACCTTCAA GGACACCGGC ATTAAAACGT GCGCAATTGA CAGATGATGA AGATGATGAC 
AATGTGGATG ATGAAGAAAG TGATTGTGTA GAGCAACGAA GAAAAAAACC TAGGATAAAC 
ACCAGGAACC AGGGCAGAAG AACTGTGTTA TATAATGATG AGTCTGATAA TGAGCATGAT 
GGTGCGGGGC AATATGATGA TCCCTTAAAC CTGGGAACAT CCAGGTCTGG AAGAGTGAGA 
AAAATGACGG AAAAGGCCAG AGTGAGTCAT CTGATGGGAT GGAATGGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

brwd3 ( XM_004916894.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004916951.1 Xenopus tropicalis bromodomain and WD repeat domain containing 3 (brwd3), transcript variant X2, mRNA.

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CloneID	OXa14412
Clone ID Related Accession (Same CDS sequence)	XM_004916894.3
Accession Version	XM_004916894.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	bromodomain and WD repeat-containing protein 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004916894.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCCG AGCTTTACTT CCTGATCGCC CGCTTCCTAC AGTCTGGCCC CTGCCGGAGA 
TCCGCACAGG TTCTGGCGGA GGAACTGGAG GAGTACCGGC TCATCCCACC GCGCTTAGAC 
TGGGAAGGGA AGGAACACCG CAGAACTTAT CAGGATTTGG TGGCAGCCAA TAAACACATC 
CCATCAGACT ACCTGCTTAA AATCTGCGAG CGTTTTGGAC CTATCCTAGA TCAAGAAATC 
CCGCAGAGTG TTCCTGGGTT GCATAGTCTG TTGGGACTTG GAAAACAGTC ACTGCTCAGA 
AGTAAAAAAG ACTGCAAGAA TACACCATGG AAAGGGACAG CTTTTGCAGC TTTGCACAGA 
GGGAGACCAC CAGAACTTCC AGTCAACTAT GGCAAGCCCC TCAACGTTGT AAATATCACT 
TGCGCCAGAT ATCAGACTGG ATATGCACAT TACCATCGCA TATTTCCATC TTCAACTTAT 
CAGCAGATGA AAATGCACAA GAGGATTTTG GGCCATCTTT CATCTGTATA CTGTGTGGCT 
TTTGATCGCA CTGGAAGACG TATTTTCACT GGTTCAGATG ACTGTCTAGT CAAAATATGG 
GCAAGTGATG ATGGACGTCT TCTTTCCACA CTACGTGGCC ATTCAGCTGA AATCTCTGAT 
CTGGCTGTGA ATCATGAGAA TACATTGATT GCTGCTGGCA GCTGTGATAA GGTGGTGCGT 
GTGTGGTGCC TTCGTACATG TGCACCAATA GCTGTGCTGC AGGGTCATAC AGCTTCTATC 
ACTTCCATAC AGTTTTGCCC AGGAACAAAA GAAAAAACAA GATACCTCAC ATCTACTGGT 
GCTGATGGGA CAATCTGCTT CTGGGAGTGG CATGCAGATA CCATGACATT TAATGATCGC 
CCTGTGAAAT TTGCAGAGAG GTCCAGGCCT GGGGTTCAGA TCTCCTGCTC ATCCTTTAGT 
TCAGGTGGTA TGTTTATAGC CACAGGAAGT ACTGATCATG TGATAAGAAT ATACTACCTG 
GGATCTGGTT CACCAGAGAA GATAGCTGAA TTGGAAGCAC ATTTGGACAA AGTGGTCGCT 
ATTCAGTTCT GCAACAATTC AGATCGATTG TGCTTTGTCA GTGGAAGCAG AGATGGCACT 
GCTCGGATTT GGCAATATCA GCAGCAAGAG TGGAAAATGA CTGTTCTGGA TATGACTGCC 
AAGATGGCTG GAAGCAGTGT TATAAATACA GAAGACAAAG TGACCAAGCT TAAAGTCACT 
ATGGTAGCAT GGGACCGATC TGATTCTTCG GTTATTACTG CTGTAAACAA TTTCTTTCTG 
AAAGTTTGGA ACTCTCATAC TGGCGAACTT CTTCATATAT TGTCTGGCCA TGATGATGAA 
GTTTTTGTTT TGGAACCTCA TCCTTTTGAT CCAAGGATTT TAATCTCTGC TGGGCATGAT 
GGAAACATAT TTGTGTGGGA CCTCGAAACA GGAACCAAAA TTCGTAATTA CTTTAACATG 
ATTGAAGGTC AGGGTCATGG AGCCGTGTTT GACTGTAAGT TCTCGCCTGA TGGTCAAAAC 
TTTGCCTGTA CAGATTCTCA TGGACATCTT TTACTCTTTG GTTTTGGTTG CAGTAAACCA 
TATGAAAAGA TTCCAGAACA GATGTTCTTC CATACAGACT TTAGACCTCT AATAAGAGAC 
TCCAACAGCT ATGTTTTGGA TGAACAAACT CAACAAGCCC CACACCTAAT GCCCCCTCCA 
TTCCTAGTAG ACGTTGATGG CAACCCACAT CCTACCCGTT TTCAGCGCTT GGTGCCAGGA 
CGTGAGAATT GTAAAGATGA GCAGCTCATA CCTCAGCTGG GATACATGTC AAATGGCAAT 
GGCGATGTGG TGGAGCAGGT CATTGGCCAG CAAACAGGAA ACCAGGAGGA AAGTATTCTT 
GATGGGATAA TCCGAGAACT CCAGAGGGAA CAGGATGAGA GGCATACAAA TGAAGCCGAG 
GATGTGGATA CACCCAGATA TAACAGAACA GACTCGAGAA ATGGAGTATT AAGAAGTATT 
AGCTTGGATT TATCATCTCC ACCTAATGTT GGCCTTCGCC GGAGTGGCCA AATTGAGGGT 
GTTCGCCAAA TGCATAGTAA TGCACCTCGG AGTCAAATTG CAACAGAAAG AGACCTAATG 
GCCTGGAGCA GAAGAGTGGT AGTAAATGAA CTGCATCCTG GTGTTATCAG GGTCCAGGAA 
GAATGCAGAA TTTCTAAAGG CGAAATGGAA ATCAGTTTGT ACAACACAGA GAAAAAGAGG 
AAACCAACTA ACCCTGCTCA GAGGAATGAT TATCAAGCTT TTGGTGGGCG ATCACTTAGA 
CGAATACAGC GGAAGCGCCA GCATGCCTAC CAGACACGTT CAACCATTGA ACATGATGCA 
AGAGCTAAAG TTCCAAGTAC AAATGGGGAA GATTCGCAGA GTTCCCCCGA GGAGGAGGAG 
TCCCAACATT CAACAGAGGC TTCAGGAGAT GAAGCTGCAG CTCAGTGGCA ATCAGAAACC 
TCTTCCAGTA ATTCATCTAG TGAATATTCC GACTGGACTG CAGATGCAGG AATCAATCTC 
CAGCCTCCCA AAAGACAGAC ACGGCAAACA ACAAGGAAAA TGTGCAGCAG TTCAGAGGAT 
GAGAACGCTA AAGAGGAAGA GAAGCCGAGG AAACACAAAC TGACCAGACG GAAGAAACAA 
AGTGAGCGTC TTTCTATGGA AGGAGAGCCA AGTGAAGAAT GGATTCCCCC CCAGTGGATC 
TTGGACACCA TCCCACGTCG TTCTCCTTTT GTTCCCCAGA TGGGAGATGA GGTTATTTAT 
TTCAGACAAG GCCATGAAGC TTATGTACGA GCAGTAAGGA AGGCTAAAAT ATATAATATT 
AATCCACAAA AGGAGCCATG GAACAAAACG GATCTCAGGG ATCAAGAATT TGCTAAGATT 
GTAGGAATTA AGTATGAGGT GGGGCCGCCT ACATTATGCT GCTTAAAACT TGCCTTACTG 
GATCCTACTT CAGGCAAAAT AACTGCAGAC TCTTTCTCTG TTAAATACCA TGACATGCCT 
GATGTTATTG ACTTCCTTGT CCTTTACCAG TTTTATAAGG AGGCGAAAGA GAGAAACTGG 
CAGATAGGAG ACCGGTTTCG AAGCATTATT GATGATGCAT GGTGGTTTGG CACAGTGGAA 
AGTCAGCAGC CATTCCAGAC TGAGTATCCT GACAGTTTAT TCCAGTGCTA CAGTGTACAC 
TGGGATAATA ATGAAAGAGA AAACATGAGT CCTTGGGACA TGGAACCAAT TCCAGATGGC 
ACTTTGCCTG ATGAAATGGG TGCTGGTGTT CCCGTTACTT CCGATGAACT GAAGTCTTTG 
CAGTATAAAC CGGAGGAGGG AGAGTGGGGA TTGTACTCAA GAGATGAGGT TTGCGAAAGA 
GTTATCCAAG GAATTGATCA GCTACTTACT CTTGATTTTG CCAGCCCATT CAGTGCTCCT 
GTGGATCTCA GTGCCTACCC CTTATACTGC ACTGTGGTAG CATACCCAAC AGATCTAAGC 
ACCACAAGGA AGAGACTGGA GAGCCACTAC TATAGGAGGC TTTCTGCCCT AATATGGGAG 
GTCCGCTATA TTGAACACAA TGCAAGAACA TTCAATGAGC CTGACACTCC AATTGTTAGA 
ACTGCCAAAA TTGTGACAGA TGTGCTGTTG CGTTATATTG GGGATGAGAA TTGTAATGAT 
ATATTAGAGA TCTACAATCA AGTAAAGACT GAAGATACTG GCAATACAGA AGATGAGGAG 
GGTGGAGCAG TTGATATGGA ATCAGATGCC CCAGGGACAT CATCTGGTCG GGCTGTTACC 
CGTCGCACTA AAAGAGATCT ACTTCGTTCC AATCCTGATT CATGGAAAGA TAAATGCAAA 
GAACTACTAG ATTTAATATA TGAGCGGGAA GATTCTGAAC CCTTTAGGCA ACCAGTCAAT 
CAAGTCTCAT ATCCTGATTA TCAGGATATT ATTGAAACAC CAATGGATTT TAGCACCGTG 
AAAACAACGT TAGAAGCTGG AAATTATAGT GACCCGTTGG AATTTGCTGC AGATGTTCGA 
CTAATCTTCA GTAACTCCAA AGCATACACT CCAAACAAAA AATCTAGAAT CTACAGTATG 
ACTCTTCGAC TCTCTGCACT ATGTGAAACC TACATAAAGG ATATCATCTC TGACTACAAA 
CTAGCCATGC AACTCCAACA GAGAACAAGG CGGAAATACA GAAGGCGAAT TAGGAGCAGC 
AGCAGCAGCA GCAGCAGCAG CAGTAGTAAT AGAAGAAGAA GTAGTAGTAG TAGTAGTAGT 
TCTAGTTCAG CATCCAGCAG AGCATCCAGC CCAGACACAA AACCTCGGAA AATGAAGCTT 
CAACCGAAAC TTAATCAGAA CCCTCCACAT CCTCTTCCTA GGAGCAGCTC TTCATCCACT 
TCTCAAATGT CTGATACTGA TGTAGAGCCT GATGGAAAGC CTTTTTCTGA AGATATGGAT 
CAAGAGCCAT CTTCATCTGG ATGCAGCAAA CCTGAAACAC ACAAAGAACA GGAGAATGTG 
ACCAAGAGCA AAAATTCTCC TGTGAAAGAG GAACATACAA ATGGCCCTCT AACAAATGGC 
GATGGAGGAA ACACACCAAA AACCAAGAGA AAGTTGCTAA GTTCTTCTGA GGATGAAAGT 
CCAGAAGATG GAGAGCAAAT GGAAGAAAAG CCTGATAAAA GTCCAGCATG TTCTCGTGAA 
AGAGACTCTG AATCAAGTTT AAGTTCGAAG AGTGAGTCAG AATCAGAGAG CAGTTCTGAT 
TCTGAATCCA TTAAGTCAGA TCAGGATTAT ATTGATGGCG ACCATCATTA TAGCAAAGAG 
CCCCGTACAA AGTTTAAGAG AAAATTTTGT AAATCTGGAG AGGCTAAAAC GAGAGGAAGG 
GGTCGAGGAA GAGGCAGAGG AAGAGGACGT GCAAAAGGTT ATAATCGTGG TGTGGAGGAA 
GAGTTAAAAA AAGGTAGGGG CCGGGGCAGA GGAAGAGGAA GAGGAGCATG GAAAAAAGAA 
CCTTCAAGGA CACCGGCATT AAAACGTGCG CAATTGACAG ATGATGAAGA TGATGACAAT 
GTGGATGATG AAGAAAGTGA TTGTGTAGAG CAACGAAGAA AAAAACCTAG GATAAACACC 
AGGAACCAGG GCAGAAGAAC TGTGTTATAT AATGATGAGT CTGATAATGA GCATGATGGT 
GCGGGGCAAT ATGATGATCC CTTAAACCTG GGAACATCCA GGTCTGGAAG AGTGAGAAAA 
ATGACGGAAA AGGCCAGAGT GAGTCATCTG ATGGGATGGA ATGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004916951.1
CDS	1168..6495
Translation	MEAELYFLIARFLQSGPCRRSAQVLAEELEEYRLIPPRLDWEGKEHRRTYQDLVAANKHIPSDYLLKICERFGPILDQEIPQSVPGLHSLLGLGKQSLLRSKKDCKNTPWKGTAFAALHRGRPPELPVNYGKPLNVVNITCARYQTGYAHYHRIFPSSTYQQMKMHKRILGHLSSVYCVAFDRTGRRIFTGSDDCLVKIWASDDGRLLSTLRGHSAEISDLAVNHENTLIAAGSCDKVVRVWCLRTCAPIAVLQGHTASITSIQFCPGTKEKTRYLTSTGADGTICFWEWHADTMTFNDRPVKFAERSRPGVQISCSSFSSGGMFIATGSTDHVIRIYYLGSGSPEKIAELEAHLDKVVAIQFCNNSDRLCFVSGSRDGTARIWQYQQQEWKMTVLDMTAKMAGSSVINTEDKVTKLKVTMVAWDRSDSSVITAVNNFFLKVWNSHTGELLHILSGHDDEVFVLEPHPFDPRILISAGHDGNIFVWDLETGTKIRNYFNMIEGQGHGAVFDCKFSPDGQNFACTDSHGHLLLFGFGCSKPYEKIPEQMFFHTDFRPLIRDSNSYVLDEQTQQAPHLMPPPFLVDVDGNPHPTRFQRLVPGRENCKDEQLIPQLGYMSNGNGDVVEQVIGQQTGNQEESILDGIIRELQREQDERHTNEAEDVDTPRYNRTDSRNGVLRSISLDLSSPPNVGLRRSGQIEGVRQMHSNAPRSQIATERDLMAWSRRVVVNELHPGVIRVQEECRISKGEMEISLYNTEKKRKPTNPAQRNDYQAFGGRSLRRIQRKRQHAYQTRSTIEHDARAKVPSTNGEDSQSSPEEEESQHSTEASGDEAAAQWQSETSSSNSSSEYSDWTADAGINLQPPKRQTRQTTRKMCSSSEDENAKEEEKPRKHKLTRRKKQSERLSMEGEPSEEWIPPQWILDTIPRRSPFVPQMGDEVIYFRQGHEAYVRAVRKAKIYNINPQKEPWNKTDLRDQEFAKIVGIKYEVGPPTLCCLKLALLDPTSGKITADSFSVKYHDMPDVIDFLVLYQFYKEAKERNWQIGDRFRSIIDDAWWFGTVESQQPFQTEYPDSLFQCYSVHWDNNERENMSPWDMEPIPDGTLPDEMGAGVPVTSDELKSLQYKPEEGEWGLYSRDEVCERVIQGIDQLLTLDFASPFSAPVDLSAYPLYCTVVAYPTDLSTTRKRLESHYYRRLSALIWEVRYIEHNARTFNEPDTPIVRTAKIVTDVLLRYIGDENCNDILEIYNQVKTEDTGNTEDEEGGAVDMESDAPGTSSGRAVTRRTKRDLLRSNPDSWKDKCKELLDLIYEREDSEPFRQPVNQVSYPDYQDIIETPMDFSTVKTTLEAGNYSDPLEFAADVRLIFSNSKAYTPNKKSRIYSMTLRLSALCETYIKDIISDYKLAMQLQQRTRRKYRRRIRSSSSSSSSSSSNRRRSSSSSSSSSSASSRASSPDTKPRKMKLQPKLNQNPPHPLPRSSSSSTSQMSDTDVEPDGKPFSEDMDQEPSSSGCSKPETHKEQENVTKSKNSPVKEEHTNGPLTNGDGGNTPKTKRKLLSSSEDESPEDGEQMEEKPDKSPACSRERDSESSLSSKSESESESSSDSESIKSDQDYIDGDHHYSKEPRTKFKRKFCKSGEAKTRGRGRGRGRGRGRAKGYNRGVEEELKKGRGRGRGRGRGAWKKEPSRTPALKRAQLTDDEDDDNVDDEESDCVEQRRKKPRINTRNQGRRTVLYNDESDNEHDGAGQYDDPLNLGTSRSGRVRKMTEKARVSHLMGWNG

Target ORF information:

RefSeq Version	XM_004916894.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis bromodomain and WD repeat domain containing 3 (brwd3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916894.3

ORF Insert Sequence:

ATGGAAGCCG AGCTTTACTT CCTGATCGCC CGCTTCCTAC AGTCTGGCCC CTGCCGGAGA 
TCCGCACAGG TTCTGGCGGA GGAACTGGAG GAGTACCGGC TCATCCCACC GCGCTTAGAC 
TGGGAAGGGA AGGAACACCG CAGAACTTAT CAGGATTTGG TGGCAGCCAA TAAACACATC 
CCATCAGACT ACCTGCTTAA AATCTGCGAG CGTTTTGGAC CTATCCTAGA TCAAGAAATC 
CCGCAGAGTG TTCCTGGGTT GCATAGTCTG TTGGGACTTG GAAAACAGTC ACTGCTCAGA 
AGTAAAAAAG ACTGCAAGAA TACACCATGG AAAGGGACAG CTTTTGCAGC TTTGCACAGA 
GGGAGACCAC CAGAACTTCC AGTCAACTAT GGCAAGCCCC TCAACGTTGT AAATATCACT 
TGCGCCAGAT ATCAGACTGG ATATGCACAT TACCATCGCA TATTTCCATC TTCAACTTAT 
CAGCAGATGA AAATGCACAA GAGGATTTTG GGCCATCTTT CATCTGTATA CTGTGTGGCT 
TTTGATCGCA CTGGAAGACG TATTTTCACT GGTTCAGATG ACTGTCTAGT CAAAATATGG 
GCAAGTGATG ATGGACGTCT TCTTTCCACA CTACGTGGCC ATTCAGCTGA AATCTCTGAT 
CTGGCTGTGA ATCATGAGAA TACATTGATT GCTGCTGGCA GCTGTGATAA GGTGGTGCGT 
GTGTGGTGCC TTCGTACATG TGCACCAATA GCTGTGCTGC AGGGTCATAC AGCTTCTATC 
ACTTCCATAC AGTTTTGCCC AGGAACAAAA GAAAAAACAA GATACCTCAC ATCTACTGGT 
GCTGATGGGA CAATCTGCTT CTGGGAGTGG CATGCAGATA CCATGACATT TAATGATCGC 
CCTGTGAAAT TTGCAGAGAG GTCCAGGCCT GGGGTTCAGA TCTCCTGCTC ATCCTTTAGT 
TCAGGTGGTA TGTTTATAGC CACAGGAAGT ACTGATCATG TGATAAGAAT ATACTACCTG 
GGATCTGGTT CACCAGAGAA GATAGCTGAA TTGGAAGCAC ATTTGGACAA AGTGGTCGCT 
ATTCAGTTCT GCAACAATTC AGATCGATTG TGCTTTGTCA GTGGAAGCAG AGATGGCACT 
GCTCGGATTT GGCAATATCA GCAGCAAGAG TGGAAAATGA CTGTTCTGGA TATGACTGCC 
AAGATGGCTG GAAGCAGTGT TATAAATACA GAAGACAAAG TGACCAAGCT TAAAGTCACT 
ATGGTAGCAT GGGACCGATC TGATTCTTCG GTTATTACTG CTGTAAACAA TTTCTTTCTG 
AAAGTTTGGA ACTCTCATAC TGGCGAACTT CTTCATATAT TGTCTGGCCA TGATGATGAA 
GTTTTTGTTT TGGAACCTCA TCCTTTTGAT CCAAGGATTT TAATCTCTGC TGGGCATGAT 
GGAAACATAT TTGTGTGGGA CCTCGAAACA GGAACCAAAA TTCGTAATTA CTTTAACATG 
ATTGAAGGTC AGGGTCATGG AGCCGTGTTT GACTGTAAGT TCTCGCCTGA TGGTCAAAAC 
TTTGCCTGTA CAGATTCTCA TGGACATCTT TTACTCTTTG GTTTTGGTTG CAGTAAACCA 
TATGAAAAGA TTCCAGAACA GATGTTCTTC CATACAGACT TTAGACCTCT AATAAGAGAC 
TCCAACAGCT ATGTTTTGGA TGAACAAACT CAACAAGCCC CACACCTAAT GCCCCCTCCA 
TTCCTAGTAG ACGTTGATGG CAACCCACAT CCTACCCGTT TTCAGCGCTT GGTGCCAGGA 
CGTGAGAATT GTAAAGATGA GCAGCTCATA CCTCAGCTGG GATACATGTC AAATGGCAAT 
GGCGATGTGG TGGAGCAGGT CATTGGCCAG CAAACAGGAA ACCAGGAGGA AAGTATTCTT 
GATGGGATAA TCCGAGAACT CCAGAGGGAA CAGGATGAGA GGCATACAAA TGAAGCCGAG 
GATGTGGATA CACCCAGATA TAACAGAACA GACTCGAGAA ATGGAGTATT AAGAAGTATT 
AGCTTGGATT TATCATCTCC ACCTAATGTT GGCCTTCGCC GGAGTGGCCA AATTGAGGGT 
GTTCGCCAAA TGCATAGTAA TGCACCTCGG AGTCAAATTG CAACAGAAAG AGACCTAATG 
GCCTGGAGCA GAAGAGTGGT AGTAAATGAA CTGCATCCTG GTGTTATCAG GGTCCAGGAA 
GAATGCAGAA TTTCTAAAGG CGAAATGGAA ATCAGTTTGT ACAACACAGA GAAAAAGAGG 
AAACCAACTA ACCCTGCTCA GAGGAATGAT TATCAAGCTT TTGGTGGGCG ATCACTTAGA 
CGAATACAGC GGAAGCGCCA GCATGCCTAC CAGACACGTT CAACCATTGA ACATGATGCA 
AGAGCTAAAG TTCCAAGTAC AAATGGGGAA GATTCGCAGA GTTCCCCCGA GGAGGAGGAG 
TCCCAACATT CAACAGAGGC TTCAGGAGAT GAAGCTGCAG CTCAGTGGCA ATCAGAAACC 
TCTTCCAGTA ATTCATCTAG TGAATATTCC GACTGGACTG CAGATGCAGG AATCAATCTC 
CAGCCTCCCA AAAGACAGAC ACGGCAAACA ACAAGGAAAA TGTGCAGCAG TTCAGAGGAT 
GAGAACGCTA AAGAGGAAGA GAAGCCGAGG AAACACAAAC TGACCAGACG GAAGAAACAA 
AGTGAGCGTC TTTCTATGGA AGGAGAGCCA AGTGAAGAAT GGATTCCCCC CCAGTGGATC 
TTGGACACCA TCCCACGTCG TTCTCCTTTT GTTCCCCAGA TGGGAGATGA GGTTATTTAT 
TTCAGACAAG GCCATGAAGC TTATGTACGA GCAGTAAGGA AGGCTAAAAT ATATAATATT 
AATCCACAAA AGGAGCCATG GAACAAAACG GATCTCAGGG ATCAAGAATT TGCTAAGATT 
GTAGGAATTA AGTATGAGGT GGGGCCGCCT ACATTATGCT GCTTAAAACT TGCCTTACTG 
GATCCTACTT CAGGCAAAAT AACTGCAGAC TCTTTCTCTG TTAAATACCA TGACATGCCT 
GATGTTATTG ACTTCCTTGT CCTTTACCAG TTTTATAAGG AGGCGAAAGA GAGAAACTGG 
CAGATAGGAG ACCGGTTTCG AAGCATTATT GATGATGCAT GGTGGTTTGG CACAGTGGAA 
AGTCAGCAGC CATTCCAGAC TGAGTATCCT GACAGTTTAT TCCAGTGCTA CAGTGTACAC 
TGGGATAATA ATGAAAGAGA AAACATGAGT CCTTGGGACA TGGAACCAAT TCCAGATGGC 
ACTTTGCCTG ATGAAATGGG TGCTGGTGTT CCCGTTACTT CCGATGAACT GAAGTCTTTG 
CAGTATAAAC CGGAGGAGGG AGAGTGGGGA TTGTACTCAA GAGATGAGGT TTGCGAAAGA 
GTTATCCAAG GAATTGATCA GCTACTTACT CTTGATTTTG CCAGCCCATT CAGTGCTCCT 
GTGGATCTCA GTGCCTACCC CTTATACTGC ACTGTGGTAG CATACCCAAC AGATCTAAGC 
ACCACAAGGA AGAGACTGGA GAGCCACTAC TATAGGAGGC TTTCTGCCCT AATATGGGAG 
GTCCGCTATA TTGAACACAA TGCAAGAACA TTCAATGAGC CTGACACTCC AATTGTTAGA 
ACTGCCAAAA TTGTGACAGA TGTGCTGTTG CGTTATATTG GGGATGAGAA TTGTAATGAT 
ATATTAGAGA TCTACAATCA AGTAAAGACT GAAGATACTG GCAATACAGA AGATGAGGAG 
GGTGGAGCAG TTGATATGGA ATCAGATGCC CCAGGGACAT CATCTGGTCG GGCTGTTACC 
CGTCGCACTA AAAGAGATCT ACTTCGTTCC AATCCTGATT CATGGAAAGA TAAATGCAAA 
GAACTACTAG ATTTAATATA TGAGCGGGAA GATTCTGAAC CCTTTAGGCA ACCAGTCAAT 
CAAGTCTCAT ATCCTGATTA TCAGGATATT ATTGAAACAC CAATGGATTT TAGCACCGTG 
AAAACAACGT TAGAAGCTGG AAATTATAGT GACCCGTTGG AATTTGCTGC AGATGTTCGA 
CTAATCTTCA GTAACTCCAA AGCATACACT CCAAACAAAA AATCTAGAAT CTACAGTATG 
ACTCTTCGAC TCTCTGCACT ATGTGAAACC TACATAAAGG ATATCATCTC TGACTACAAA 
CTAGCCATGC AACTCCAACA GAGAACAAGG CGGAAATACA GAAGGCGAAT TAGGAGCAGC 
AGCAGCAGCA GCAGCAGCAG CAGTAGTAAT AGAAGAAGAA GTAGTAGTAG TAGTAGTAGT 
TCTAGTTCAG CATCCAGCAG AGCATCCAGC CCAGACACAA AACCTCGGAA AATGAAGCTT 
CAACCGAAAC TTAATCAGAA CCCTCCACAT CCTCTTCCTA GGAGCAGCTC TTCATCCACT 
TCTCAAATGT CTGATACTGA TGTAGAGCCT GATGGAAAGC CTTTTTCTGA AGATATGGAT 
CAAGAGCCAT CTTCATCTGG ATGCAGCAAA CCTGAAACAC ACAAAGAACA GGAGAATGTG 
ACCAAGAGCA AAAATTCTCC TGTGAAAGAG GAACATACAA ATGGCCCTCT AACAAATGGC 
GATGGAGGAA ACACACCAAA AACCAAGAGA AAGTTGCTAA GTTCTTCTGA GGATGAAAGT 
CCAGAAGATG GAGAGCAAAT GGAAGAAAAG CCTGATAAAA GTCCAGCATG TTCTCGTGAA 
AGAGACTCTG AATCAAGTTT AAGTTCGAAG AGTGAGTCAG AATCAGAGAG CAGTTCTGAT 
TCTGAATCCA TTAAGTCAGA TCAGGATTAT ATTGATGGCG ACCATCATTA TAGCAAAGAG 
CCCCGTACAA AGTTTAAGAG AAAATTTTGT AAATCTGGAG AGGCTAAAAC GAGAGGAAGG 
GGTCGAGGAA GAGGCAGAGG AAGAGGACGT GCAAAAGGTT ATAATCGTGG TGTGGAGGAA 
GAGTTAAAAA AAGGTAGGGG CCGGGGCAGA GGAAGAGGAA GAGGAGCATG GAAAAAAGAA 
CCTTCAAGGA CACCGGCATT AAAACGTGCG CAATTGACAG ATGATGAAGA TGATGACAAT 
GTGGATGATG AAGAAAGTGA TTGTGTAGAG CAACGAAGAA AAAAACCTAG GATAAACACC 
AGGAACCAGG GCAGAAGAAC TGTGTTATAT AATGATGAGT CTGATAATGA GCATGATGGT 
GCGGGGCAAT ATGATGATCC CTTAAACCTG GGAACATCCA GGTCTGGAAG AGTGAGAAAA 
ATGACGGAAA AGGCCAGAGT GAGTCATCTG ATGGGATGGA ATGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

brwd3 ( XM_018096621.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017952110.1 Xenopus tropicalis bromodomain and WD repeat domain containing 3 (brwd3), transcript variant X3, mRNA.

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CloneID	OXa46289
Clone ID Related Accession (Same CDS sequence)	XM_018096621.1
Accession Version	XM_018096621.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5313bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	bromodomain and WD repeat-containing protein 3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCCG AGCTTTACTT CCTGATCGCC CGCTTCCTAC AGTCTGGCCC CTGCCGGAGA 
TCCGCACAGG TTCTGGCGGA GGAACTGGAG GAGTACCGGC TCATCCCACC GCGCTTAGAC 
TGGGAAGGGA AGGAACACCG CAGAACTTAT CAGGATTTGG TGGCAGCCAA TAAACACATC 
CCATCAGACT ACCTGCTTAA AATCTGCGAG CGTTTTGGAC CTATCCTAGA TCAAGAAATC 
CCGCAGAGTG TTCCTGGGTT GCATAGTCTG TTGGGACTTG GAAAACAGTC ACTGCTCAGA 
AGTAAAAAAG ACTGCAAGAA TACACCATGG AAAGGGACAG CTTTTGCAGC TTTGCACAGA 
GGGAGACCAC CAGAACTTCC AGTCAACTAT GGCAAGCCCC TCAACGTTGT AAATATCACT 
TGCGCCAGAT ATCAGACTGG ATATGCACAT TACCATCGCA TATTTCCATC TTCAACTTAT 
CAGCAGATGA AAATGCACAA GAGGATTTTG GGCCATCTTT CATCTGTATA CTGTGTGGCT 
TTTGATCGCA CTGGAAGACG TATTTTCACT GGTTCAGATG ACTGTCTAGT CAAAATATGG 
GCAAGTGATG ATGGACGTCT TCTTTCCACA CTACGTGGCC ATTCAGCTGA AATCTCTGAT 
CTGGCTGTGA ATCATGAGAA TACATTGATT GCTGCTGGCA GCTGTGATAA GGTGGTGCGT 
GTGTGGTGCC TTCGTACATG TGCACCAATA GCTGTGCTGC AGGGTCATAC AGCTTCTATC 
ACTTCCATAC AGTTTTGCCC AGGAACAAAA GAAAAAACAA GATACCTCAC ATCTACTGGT 
GCTGATGGGA CAATCTGCTT CTGGGAGTGG CATGCAGATA CCATGACATT TAATGATCGC 
CCTGTGAAAT TTGCAGAGAG GTCCAGGCCT GGGGTTCAGA TCTCCTGCTC ATCCTTTAGT 
TCAGGTGGTA TGTTTATAGC CACAGGAAGT ACTGATCATG TGATAAGAAT ATACTACCTG 
GGATCTGGTT CACCAGAGAA GATAGCTGAA TTGGAAGCAC ATTTGGACAA AGTGGTCGCT 
ATTCAGTTCT GCAACAATTC AGATCGATTG TGCTTTGTCA GTGGAAGCAG AGATGGCACT 
GCTCGGATTT GGCAATATCA GCAGCAAGAG TGGAAAATGA CTGTTCTGGA TATGACTGCC 
AAGATGGCTG GAAGCAGTGT TATAAATACA GAAGACAAAG TGACCAAGCT TAAAGTCACT 
ATGGTAGCAT GGGACCGATC TGATTCTTCG GTTATTACTG CTGTAAACAA TTTCTTTCTG 
AAAGTTTGGA ACTCTCATAC TGGCGAACTT CTTCATATAT TGTCTGGCCA TGATGATGAA 
GTTTTTGTTT TGGAACCTCA TCCTTTTGAT CCAAGGATTT TAATCTCTGC TGGGCATGAT 
GGAAACATAT TTGTGTGGGA CCTCGAAACA GGAACCAAAA TTCGTAATTA CTTTAACATG 
ATTGAAGGTC AGGGTCATGG AGCCGTGTTT GACTGTAAGT TCTCGCCTGA TGGTCAAAAC 
TTTGCCTGTA CAGATTCTCA TGGACATCTT TTACTCTTTG GTTTTGGTTG CAGTAAACCA 
TATGAAAAGA TTCCAGAACA GATGTTCTTC CATACAGACT TTAGACCTCT AATAAGAGAC 
TCCAACAGCT ATGTTTTGGA TGAACAAACT CAACAAGCCC CACACCTAAT GCCCCCTCCA 
TTCCTAGTAG ACGTTGATGG CAACCCACAT CCTACCCGTT TTCAGCGCTT GGTGCCAGGA 
CGTGAGAATT GTAAAGATGA GCAGCTCATA CCTCAGCTGG GATACATGTC AAATGGCAAT 
GGCGATGTGG TGGAGCAGGT CATTGGCCAG CAAACAGGAA ACCAGGAGGA AAGTATTCTT 
GATGGGATAA TCCGAGAACT CCAGAGGGAA CAGGATGAGA GGCATACAAA TGAAGCCGAG 
GATGTGGATA CACCCAGATA TAACAGAACA GACTCGAGAA ATGGAGTATT AAGAAGTATT 
AGCTTGGATT TATCATCTCC ACCTAATGTT GGCCTTCGCC GGAGTGGCCA AATTGAGGGT 
GTTCGCCAAA TGCATAGTAA TGCACCTCGG AGTCAAATTG CAACAGAAAG AGACCTAATG 
GCCTGGAGCA GAAGAGTGGT AGTAAATGAA CTGCATCCTG GTGTTATCAG GGTCCAGGAA 
GAATGCAGAA TTTCTAAAGG CGAAATGGAA ATCAGTTTGT ACAACACAGA GAAAAAGAGG 
AAACCAACTA ACCCTGCTCA GAGGAATGAT TATCAAGCTT TTGGTGGGCG ATCACTTAGA 
CGAATACAGC GGAAGCGCCA GCATGCCTAC CAGACACGTT CAACCATTGA ACATGATGCA 
AGAGCTAAAG TTCCAAGTAC AAATGGGGAA GATTCGCAGA GTTCCCCCGA GGAGGAGGAG 
TCCCAACATT CAACAGAGGC TTCAGGAGAT GAAGCTGCAG CTCAGTGGCA ATCAGAAACC 
TCTTCCAGTA ATTCATCTAG TGAATATTCC GACTGGACTG CAGATGCAGG AATCAATCTC 
CAGCCTCCCA AAAGACAGAC ACGGCAAACA ACAAGGAAAA TGTGCAGCAG TTCAGAGGAT 
GAGAACGCTA AAGAGGAAGA GAAGCCGAGG AAACACAAAC TGACCAGACG GAAGAAACAA 
AGTGAGCGTC TTTCTATGGA AGGAGAGCCA AGTGAAGAAT GGATTCCCCC CCAGTGGATC 
TTGGACACCA TCCCACGTCG TTCTCCTTTT GTTCCCCAGA TGGGAGATGA GGTTATTTAT 
TTCAGACAAG GCCATGAAGC TTATGTACGA GCAGTAAGGA AGGCTAAAAT ATATAATATT 
AATCCACAAA AGGAGCCATG GAACAAAACG GATCTCAGGG ATCAAGAATT TGCTAAGATT 
GTAGGAATTA AGTATGAGGT GGGGCCGCCT ACATTATGCT GCTTAAAACT TGCCTTACTG 
GATCCTACTT CAGGCAAAAT AACTGCAGAC TCTTTCTCTG TTAAATACCA TGACATGCCT 
GATGTTATTG ACTTCCTTGT CCTTTACCAG TTTTATAAGG AGGCGAAAGA GAGAAACTGG 
CAGATAGGAG ACCGGTTTCG AAGCATTATT GATGATGCAT GGTGGTTTGG CACAGTGGAA 
AGTCAGCAGC CATTCCAGAC TGAGTATCCT GACAGTTTAT TCCAGTGCTA CAGTGTACAC 
TGGGATAATA ATGAAAGAGA AAACATGAGT CCTTGGGACA TGGAACCAAT TCCAGATGGC 
ACAGCTTTGC CTGATGAAAT GGGTGCTGGT GTTCCCGTTA CTTCCGATGA ACTGAAGTCT 
TTGCAGTATA AACCGGAGGA GGGAGAGTGG GGATTGTACT CAAGAGATGA GGTTTGCGAA 
AGAGTTATCC AAGGAATTGA TCAGCTACTT ACTCTTGATT TTGCCAGCCC ATTCAGTGCT 
CCTGTGGATC TCAGTGCCTA CCCCTTATAC TGCACTGTGG TAGCATACCC AACAGATCTA 
AGCACCACAA GGAAGAGACT GGAGAGCCAC TACTATAGGA GGCTTTCTGC CCTAATATGG 
GAGGTCCGCT ATATTGAACA CAATGCAAGA ACATTCAATG AGCCTGACAC TCCAATTGTT 
AGAACTGCCA AAATTGTGAC AGATGTGCTG TTGCGTTATA TTGGGGATGA GAATTGTAAT 
GATATATTAG AGATCTACAA TCAAGTAAAG ACTGAAGATA CTGGCAATAC AGAAGATGAG 
GAGGGTGGAG CAGTTGATAT GGAATCAGAT GCCCCAGGGA CATCATCTGG TCGGGCTGTT 
ACCCGTCGCA CTAAAAGAGA TCTACTTCGT TCCAATCCTG ATTCATGGAA AGATAAATGC 
AAAGAACTAC TAGATTTAAT ATATGAGCGG GAAGATTCTG AACCCTTTAG GCAACCAGTC 
AATCAAGTCT CATATCCTGA TTATCAGGAT ATTATTGAAA CACCAATGGA TTTTAGCACC 
GTGAAAACAA CGTTAGAAGC TGGAAATTAT AGTGACCCGT TGGAATTTGC TGCAGATGTT 
CGACTAATCT TCAGTAACTC CAAAGCATAC ACTCCAAACA AAAAATCTAG AATCTACAGT 
ATGACTCTTC GACTCTCTGC ACTATGTGAA ACCTACATAA AGGATATCAT CTCTGACTAC 
AAACTAGCCA TGCAACTCCA ACAGAGAACA AGGCGGAAAT ACAGAAGGCG AATTAGGAGC 
AGCAGCAGCA GCAGCAGCAG CAGCAGTAGT AATAGAAGAA GAAGTAGTAG TAGTAGTAGT 
AGTTCTAGTT CAGCATCCAG CAGAGCATCC AGCCCAGACA CAAAACCTCG GAAAATGAAG 
CTTCAACCGA AACTTAATCA GAACCCTCCA CATCCTCTTC CTAGGAGCAG CTCTTCATCC 
ACTTCTCAAA AGCCTGATGG AAAGCCTTTT TCTGAAGATA TGGATCAAGA GCCATCTTCA 
TCTGGATGCA GCAAACCTGA AACACACAAA GAACAGGAGA ATGTGACCAA GAGCAAAAAT 
TCTCCTGTGA AAGAGGAACA TACAAATGGC CCTCTAACAA ATGGCGATGG AGGAAACACA 
CCAAAAACCA AGAGAAAGTT GCTAAGTTCT TCTGAGGATG AAAGTCCAGA AGATGGAGAG 
CAAATGGAAG AAAAGCCTGA TAAAAGTCCA GCATGTTCTC GTGAAAGAGA CTCTGAATCA 
AGTTTAAGTT CGAAGAGTGA GTCAGAATCA GAGAGCAGTT CTGATTCTGA ATCCATTAAG 
TCAGATCAGG ATTATATTGA TGGCGACCAT CATTATAGCA AAGAGCCCCG TACAAAGTTT 
AAGAGAAAAT TTTGTAAATC TGGAGAGGCT AAAACGAGAG GAAGGGGTCG AGGAAGAGGC 
AGAGGAAGAG GACGTGCAAA AGGTTATAAT CGTGGTGTGG AGGAAGAGTT AAAAAAAGGT 
AGGGGCCGGG GCAGAGGAAG AGGAAGAGGA GCATGGAAAA AAGAACCTTC AAGGACACCG 
GCATTAAAAC GTGCGCAATT GACAGATGAT GAAGATGATG ACAATGTGGA TGATGAAGAA 
AGTGATTGTG TAGAGCAACG AAGAAAAAAA CCTAGGATAA ACACCAGGAA CCAGGGCAGA 
AGAACTGTGT TATATAATGA TGAGTCTGAT AATGAGCATG ATGGTGCGGG GCAATATGAT 
GATCCCTTAA ACCTGGGAAC ATCCAGGTCT GGAAGAGTGA GAAAAATGAC GGAAAAGGCC 
AGAGTGAGTC ATCTGATGGG ATGGAATGGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017952110.1
CDS	1171..6483
Translation	MEAELYFLIARFLQSGPCRRSAQVLAEELEEYRLIPPRLDWEGKEHRRTYQDLVAANKHIPSDYLLKICERFGPILDQEIPQSVPGLHSLLGLGKQSLLRSKKDCKNTPWKGTAFAALHRGRPPELPVNYGKPLNVVNITCARYQTGYAHYHRIFPSSTYQQMKMHKRILGHLSSVYCVAFDRTGRRIFTGSDDCLVKIWASDDGRLLSTLRGHSAEISDLAVNHENTLIAAGSCDKVVRVWCLRTCAPIAVLQGHTASITSIQFCPGTKEKTRYLTSTGADGTICFWEWHADTMTFNDRPVKFAERSRPGVQISCSSFSSGGMFIATGSTDHVIRIYYLGSGSPEKIAELEAHLDKVVAIQFCNNSDRLCFVSGSRDGTARIWQYQQQEWKMTVLDMTAKMAGSSVINTEDKVTKLKVTMVAWDRSDSSVITAVNNFFLKVWNSHTGELLHILSGHDDEVFVLEPHPFDPRILISAGHDGNIFVWDLETGTKIRNYFNMIEGQGHGAVFDCKFSPDGQNFACTDSHGHLLLFGFGCSKPYEKIPEQMFFHTDFRPLIRDSNSYVLDEQTQQAPHLMPPPFLVDVDGNPHPTRFQRLVPGRENCKDEQLIPQLGYMSNGNGDVVEQVIGQQTGNQEESILDGIIRELQREQDERHTNEAEDVDTPRYNRTDSRNGVLRSISLDLSSPPNVGLRRSGQIEGVRQMHSNAPRSQIATERDLMAWSRRVVVNELHPGVIRVQEECRISKGEMEISLYNTEKKRKPTNPAQRNDYQAFGGRSLRRIQRKRQHAYQTRSTIEHDARAKVPSTNGEDSQSSPEEEESQHSTEASGDEAAAQWQSETSSSNSSSEYSDWTADAGINLQPPKRQTRQTTRKMCSSSEDENAKEEEKPRKHKLTRRKKQSERLSMEGEPSEEWIPPQWILDTIPRRSPFVPQMGDEVIYFRQGHEAYVRAVRKAKIYNINPQKEPWNKTDLRDQEFAKIVGIKYEVGPPTLCCLKLALLDPTSGKITADSFSVKYHDMPDVIDFLVLYQFYKEAKERNWQIGDRFRSIIDDAWWFGTVESQQPFQTEYPDSLFQCYSVHWDNNERENMSPWDMEPIPDGTALPDEMGAGVPVTSDELKSLQYKPEEGEWGLYSRDEVCERVIQGIDQLLTLDFASPFSAPVDLSAYPLYCTVVAYPTDLSTTRKRLESHYYRRLSALIWEVRYIEHNARTFNEPDTPIVRTAKIVTDVLLRYIGDENCNDILEIYNQVKTEDTGNTEDEEGGAVDMESDAPGTSSGRAVTRRTKRDLLRSNPDSWKDKCKELLDLIYEREDSEPFRQPVNQVSYPDYQDIIETPMDFSTVKTTLEAGNYSDPLEFAADVRLIFSNSKAYTPNKKSRIYSMTLRLSALCETYIKDIISDYKLAMQLQQRTRRKYRRRIRSSSSSSSSSSSNRRRSSSSSSSSSSASSRASSPDTKPRKMKLQPKLNQNPPHPLPRSSSSSTSQKPDGKPFSEDMDQEPSSSGCSKPETHKEQENVTKSKNSPVKEEHTNGPLTNGDGGNTPKTKRKLLSSSEDESPEDGEQMEEKPDKSPACSRERDSESSLSSKSESESESSSDSESIKSDQDYIDGDHHYSKEPRTKFKRKFCKSGEAKTRGRGRGRGRGRGRAKGYNRGVEEELKKGRGRGRGRGRGAWKKEPSRTPALKRAQLTDDEDDDNVDDEESDCVEQRRKKPRINTRNQGRRTVLYNDESDNEHDGAGQYDDPLNLGTSRSGRVRKMTEKARVSHLMGWNG

Target ORF information:

RefSeq Version	XM_018096621.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis bromodomain and WD repeat domain containing 3 (brwd3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096621.1

ORF Insert Sequence:

ATGGAAGCCG AGCTTTACTT CCTGATCGCC CGCTTCCTAC AGTCTGGCCC CTGCCGGAGA 
TCCGCACAGG TTCTGGCGGA GGAACTGGAG GAGTACCGGC TCATCCCACC GCGCTTAGAC 
TGGGAAGGGA AGGAACACCG CAGAACTTAT CAGGATTTGG TGGCAGCCAA TAAACACATC 
CCATCAGACT ACCTGCTTAA AATCTGCGAG CGTTTTGGAC CTATCCTAGA TCAAGAAATC 
CCGCAGAGTG TTCCTGGGTT GCATAGTCTG TTGGGACTTG GAAAACAGTC ACTGCTCAGA 
AGTAAAAAAG ACTGCAAGAA TACACCATGG AAAGGGACAG CTTTTGCAGC TTTGCACAGA 
GGGAGACCAC CAGAACTTCC AGTCAACTAT GGCAAGCCCC TCAACGTTGT AAATATCACT 
TGCGCCAGAT ATCAGACTGG ATATGCACAT TACCATCGCA TATTTCCATC TTCAACTTAT 
CAGCAGATGA AAATGCACAA GAGGATTTTG GGCCATCTTT CATCTGTATA CTGTGTGGCT 
TTTGATCGCA CTGGAAGACG TATTTTCACT GGTTCAGATG ACTGTCTAGT CAAAATATGG 
GCAAGTGATG ATGGACGTCT TCTTTCCACA CTACGTGGCC ATTCAGCTGA AATCTCTGAT 
CTGGCTGTGA ATCATGAGAA TACATTGATT GCTGCTGGCA GCTGTGATAA GGTGGTGCGT 
GTGTGGTGCC TTCGTACATG TGCACCAATA GCTGTGCTGC AGGGTCATAC AGCTTCTATC 
ACTTCCATAC AGTTTTGCCC AGGAACAAAA GAAAAAACAA GATACCTCAC ATCTACTGGT 
GCTGATGGGA CAATCTGCTT CTGGGAGTGG CATGCAGATA CCATGACATT TAATGATCGC 
CCTGTGAAAT TTGCAGAGAG GTCCAGGCCT GGGGTTCAGA TCTCCTGCTC ATCCTTTAGT 
TCAGGTGGTA TGTTTATAGC CACAGGAAGT ACTGATCATG TGATAAGAAT ATACTACCTG 
GGATCTGGTT CACCAGAGAA GATAGCTGAA TTGGAAGCAC ATTTGGACAA AGTGGTCGCT 
ATTCAGTTCT GCAACAATTC AGATCGATTG TGCTTTGTCA GTGGAAGCAG AGATGGCACT 
GCTCGGATTT GGCAATATCA GCAGCAAGAG TGGAAAATGA CTGTTCTGGA TATGACTGCC 
AAGATGGCTG GAAGCAGTGT TATAAATACA GAAGACAAAG TGACCAAGCT TAAAGTCACT 
ATGGTAGCAT GGGACCGATC TGATTCTTCG GTTATTACTG CTGTAAACAA TTTCTTTCTG 
AAAGTTTGGA ACTCTCATAC TGGCGAACTT CTTCATATAT TGTCTGGCCA TGATGATGAA 
GTTTTTGTTT TGGAACCTCA TCCTTTTGAT CCAAGGATTT TAATCTCTGC TGGGCATGAT 
GGAAACATAT TTGTGTGGGA CCTCGAAACA GGAACCAAAA TTCGTAATTA CTTTAACATG 
ATTGAAGGTC AGGGTCATGG AGCCGTGTTT GACTGTAAGT TCTCGCCTGA TGGTCAAAAC 
TTTGCCTGTA CAGATTCTCA TGGACATCTT TTACTCTTTG GTTTTGGTTG CAGTAAACCA 
TATGAAAAGA TTCCAGAACA GATGTTCTTC CATACAGACT TTAGACCTCT AATAAGAGAC 
TCCAACAGCT ATGTTTTGGA TGAACAAACT CAACAAGCCC CACACCTAAT GCCCCCTCCA 
TTCCTAGTAG ACGTTGATGG CAACCCACAT CCTACCCGTT TTCAGCGCTT GGTGCCAGGA 
CGTGAGAATT GTAAAGATGA GCAGCTCATA CCTCAGCTGG GATACATGTC AAATGGCAAT 
GGCGATGTGG TGGAGCAGGT CATTGGCCAG CAAACAGGAA ACCAGGAGGA AAGTATTCTT 
GATGGGATAA TCCGAGAACT CCAGAGGGAA CAGGATGAGA GGCATACAAA TGAAGCCGAG 
GATGTGGATA CACCCAGATA TAACAGAACA GACTCGAGAA ATGGAGTATT AAGAAGTATT 
AGCTTGGATT TATCATCTCC ACCTAATGTT GGCCTTCGCC GGAGTGGCCA AATTGAGGGT 
GTTCGCCAAA TGCATAGTAA TGCACCTCGG AGTCAAATTG CAACAGAAAG AGACCTAATG 
GCCTGGAGCA GAAGAGTGGT AGTAAATGAA CTGCATCCTG GTGTTATCAG GGTCCAGGAA 
GAATGCAGAA TTTCTAAAGG CGAAATGGAA ATCAGTTTGT ACAACACAGA GAAAAAGAGG 
AAACCAACTA ACCCTGCTCA GAGGAATGAT TATCAAGCTT TTGGTGGGCG ATCACTTAGA 
CGAATACAGC GGAAGCGCCA GCATGCCTAC CAGACACGTT CAACCATTGA ACATGATGCA 
AGAGCTAAAG TTCCAAGTAC AAATGGGGAA GATTCGCAGA GTTCCCCCGA GGAGGAGGAG 
TCCCAACATT CAACAGAGGC TTCAGGAGAT GAAGCTGCAG CTCAGTGGCA ATCAGAAACC 
TCTTCCAGTA ATTCATCTAG TGAATATTCC GACTGGACTG CAGATGCAGG AATCAATCTC 
CAGCCTCCCA AAAGACAGAC ACGGCAAACA ACAAGGAAAA TGTGCAGCAG TTCAGAGGAT 
GAGAACGCTA AAGAGGAAGA GAAGCCGAGG AAACACAAAC TGACCAGACG GAAGAAACAA 
AGTGAGCGTC TTTCTATGGA AGGAGAGCCA AGTGAAGAAT GGATTCCCCC CCAGTGGATC 
TTGGACACCA TCCCACGTCG TTCTCCTTTT GTTCCCCAGA TGGGAGATGA GGTTATTTAT 
TTCAGACAAG GCCATGAAGC TTATGTACGA GCAGTAAGGA AGGCTAAAAT ATATAATATT 
AATCCACAAA AGGAGCCATG GAACAAAACG GATCTCAGGG ATCAAGAATT TGCTAAGATT 
GTAGGAATTA AGTATGAGGT GGGGCCGCCT ACATTATGCT GCTTAAAACT TGCCTTACTG 
GATCCTACTT CAGGCAAAAT AACTGCAGAC TCTTTCTCTG TTAAATACCA TGACATGCCT 
GATGTTATTG ACTTCCTTGT CCTTTACCAG TTTTATAAGG AGGCGAAAGA GAGAAACTGG 
CAGATAGGAG ACCGGTTTCG AAGCATTATT GATGATGCAT GGTGGTTTGG CACAGTGGAA 
AGTCAGCAGC CATTCCAGAC TGAGTATCCT GACAGTTTAT TCCAGTGCTA CAGTGTACAC 
TGGGATAATA ATGAAAGAGA AAACATGAGT CCTTGGGACA TGGAACCAAT TCCAGATGGC 
ACAGCTTTGC CTGATGAAAT GGGTGCTGGT GTTCCCGTTA CTTCCGATGA ACTGAAGTCT 
TTGCAGTATA AACCGGAGGA GGGAGAGTGG GGATTGTACT CAAGAGATGA GGTTTGCGAA 
AGAGTTATCC AAGGAATTGA TCAGCTACTT ACTCTTGATT TTGCCAGCCC ATTCAGTGCT 
CCTGTGGATC TCAGTGCCTA CCCCTTATAC TGCACTGTGG TAGCATACCC AACAGATCTA 
AGCACCACAA GGAAGAGACT GGAGAGCCAC TACTATAGGA GGCTTTCTGC CCTAATATGG 
GAGGTCCGCT ATATTGAACA CAATGCAAGA ACATTCAATG AGCCTGACAC TCCAATTGTT 
AGAACTGCCA AAATTGTGAC AGATGTGCTG TTGCGTTATA TTGGGGATGA GAATTGTAAT 
GATATATTAG AGATCTACAA TCAAGTAAAG ACTGAAGATA CTGGCAATAC AGAAGATGAG 
GAGGGTGGAG CAGTTGATAT GGAATCAGAT GCCCCAGGGA CATCATCTGG TCGGGCTGTT 
ACCCGTCGCA CTAAAAGAGA TCTACTTCGT TCCAATCCTG ATTCATGGAA AGATAAATGC 
AAAGAACTAC TAGATTTAAT ATATGAGCGG GAAGATTCTG AACCCTTTAG GCAACCAGTC 
AATCAAGTCT CATATCCTGA TTATCAGGAT ATTATTGAAA CACCAATGGA TTTTAGCACC 
GTGAAAACAA CGTTAGAAGC TGGAAATTAT AGTGACCCGT TGGAATTTGC TGCAGATGTT 
CGACTAATCT TCAGTAACTC CAAAGCATAC ACTCCAAACA AAAAATCTAG AATCTACAGT 
ATGACTCTTC GACTCTCTGC ACTATGTGAA ACCTACATAA AGGATATCAT CTCTGACTAC 
AAACTAGCCA TGCAACTCCA ACAGAGAACA AGGCGGAAAT ACAGAAGGCG AATTAGGAGC 
AGCAGCAGCA GCAGCAGCAG CAGCAGTAGT AATAGAAGAA GAAGTAGTAG TAGTAGTAGT 
AGTTCTAGTT CAGCATCCAG CAGAGCATCC AGCCCAGACA CAAAACCTCG GAAAATGAAG 
CTTCAACCGA AACTTAATCA GAACCCTCCA CATCCTCTTC CTAGGAGCAG CTCTTCATCC 
ACTTCTCAAA AGCCTGATGG AAAGCCTTTT TCTGAAGATA TGGATCAAGA GCCATCTTCA 
TCTGGATGCA GCAAACCTGA AACACACAAA GAACAGGAGA ATGTGACCAA GAGCAAAAAT 
TCTCCTGTGA AAGAGGAACA TACAAATGGC CCTCTAACAA ATGGCGATGG AGGAAACACA 
CCAAAAACCA AGAGAAAGTT GCTAAGTTCT TCTGAGGATG AAAGTCCAGA AGATGGAGAG 
CAAATGGAAG AAAAGCCTGA TAAAAGTCCA GCATGTTCTC GTGAAAGAGA CTCTGAATCA 
AGTTTAAGTT CGAAGAGTGA GTCAGAATCA GAGAGCAGTT CTGATTCTGA ATCCATTAAG 
TCAGATCAGG ATTATATTGA TGGCGACCAT CATTATAGCA AAGAGCCCCG TACAAAGTTT 
AAGAGAAAAT TTTGTAAATC TGGAGAGGCT AAAACGAGAG GAAGGGGTCG AGGAAGAGGC 
AGAGGAAGAG GACGTGCAAA AGGTTATAAT CGTGGTGTGG AGGAAGAGTT AAAAAAAGGT 
AGGGGCCGGG GCAGAGGAAG AGGAAGAGGA GCATGGAAAA AAGAACCTTC AAGGACACCG 
GCATTAAAAC GTGCGCAATT GACAGATGAT GAAGATGATG ACAATGTGGA TGATGAAGAA 
AGTGATTGTG TAGAGCAACG AAGAAAAAAA CCTAGGATAA ACACCAGGAA CCAGGGCAGA 
AGAACTGTGT TATATAATGA TGAGTCTGAT AATGAGCATG ATGGTGCGGG GCAATATGAT 
GATCCCTTAA ACCTGGGAAC ATCCAGGTCT GGAAGAGTGA GAAAAATGAC GGAAAAGGCC 
AGAGTGAGTC ATCTGATGGG ATGGAATGGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

brwd3 ( XM_002940436.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002940482.3 Xenopus tropicalis bromodomain and WD repeat domain containing 3 (brwd3), transcript variant X1, mRNA.

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CloneID	OXa64563
Clone ID Related Accession (Same CDS sequence)	XM_002940436.5
Accession Version	XM_002940436.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5337bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	bromodomain and WD repeat-containing protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002940436.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCCG AGCTTTACTT CCTGATCGCC CGCTTCCTAC AGTCTGGCCC CTGCCGGAGA 
TCCGCACAGG TTCTGGCGGA GGAACTGGAG GAGTACCGGC TCATCCCACC GCGCTTAGAC 
TGGGAAGGGA AGGAACACCG CAGAACTTAT CAGGATTTGG TGGCAGCCAA TAAACACATC 
CCATCAGACT ACCTGCTTAA AATCTGCGAG CGTTTTGGAC CTATCCTAGA TCAAGAAATC 
CCGCAGAGTG TTCCTGGGTT GCATAGTCTG TTGGGACTTG GAAAACAGTC ACTGCTCAGA 
AGTAAAAAAG ACTGCAAGAA TACACCATGG AAAGGGACAG CTTTTGCAGC TTTGCACAGA 
GGGAGACCAC CAGAACTTCC AGTCAACTAT GGCAAGCCCC TCAACGTTGT AAATATCACT 
TGCGCCAGAT ATCAGACTGG ATATGCACAT TACCATCGCA TATTTCCATC TTCAACTTAT 
CAGCAGATGA AAATGCACAA GAGGATTTTG GGCCATCTTT CATCTGTATA CTGTGTGGCT 
TTTGATCGCA CTGGAAGACG TATTTTCACT GGTTCAGATG ACTGTCTAGT CAAAATATGG 
GCAAGTGATG ATGGACGTCT TCTTTCCACA CTACGTGGCC ATTCAGCTGA AATCTCTGAT 
CTGGCTGTGA ATCATGAGAA TACATTGATT GCTGCTGGCA GCTGTGATAA GGTGGTGCGT 
GTGTGGTGCC TTCGTACATG TGCACCAATA GCTGTGCTGC AGGGTCATAC AGCTTCTATC 
ACTTCCATAC AGTTTTGCCC AGGAACAAAA GAAAAAACAA GATACCTCAC ATCTACTGGT 
GCTGATGGGA CAATCTGCTT CTGGGAGTGG CATGCAGATA CCATGACATT TAATGATCGC 
CCTGTGAAAT TTGCAGAGAG GTCCAGGCCT GGGGTTCAGA TCTCCTGCTC ATCCTTTAGT 
TCAGGTGGTA TGTTTATAGC CACAGGAAGT ACTGATCATG TGATAAGAAT ATACTACCTG 
GGATCTGGTT CACCAGAGAA GATAGCTGAA TTGGAAGCAC ATTTGGACAA AGTGGTCGCT 
ATTCAGTTCT GCAACAATTC AGATCGATTG TGCTTTGTCA GTGGAAGCAG AGATGGCACT 
GCTCGGATTT GGCAATATCA GCAGCAAGAG TGGAAAATGA CTGTTCTGGA TATGACTGCC 
AAGATGGCTG GAAGCAGTGT TATAAATACA GAAGACAAAG TGACCAAGCT TAAAGTCACT 
ATGGTAGCAT GGGACCGATC TGATTCTTCG GTTATTACTG CTGTAAACAA TTTCTTTCTG 
AAAGTTTGGA ACTCTCATAC TGGCGAACTT CTTCATATAT TGTCTGGCCA TGATGATGAA 
GTTTTTGTTT TGGAACCTCA TCCTTTTGAT CCAAGGATTT TAATCTCTGC TGGGCATGAT 
GGAAACATAT TTGTGTGGGA CCTCGAAACA GGAACCAAAA TTCGTAATTA CTTTAACATG 
ATTGAAGGTC AGGGTCATGG AGCCGTGTTT GACTGTAAGT TCTCGCCTGA TGGTCAAAAC 
TTTGCCTGTA CAGATTCTCA TGGACATCTT TTACTCTTTG GTTTTGGTTG CAGTAAACCA 
TATGAAAAGA TTCCAGAACA GATGTTCTTC CATACAGACT TTAGACCTCT AATAAGAGAC 
TCCAACAGCT ATGTTTTGGA TGAACAAACT CAACAAGCCC CACACCTAAT GCCCCCTCCA 
TTCCTAGTAG ACGTTGATGG CAACCCACAT CCTACCCGTT TTCAGCGCTT GGTGCCAGGA 
CGTGAGAATT GTAAAGATGA GCAGCTCATA CCTCAGCTGG GATACATGTC AAATGGCAAT 
GGCGATGTGG TGGAGCAGGT CATTGGCCAG CAAACAGGAA ACCAGGAGGA AAGTATTCTT 
GATGGGATAA TCCGAGAACT CCAGAGGGAA CAGGATGAGA GGCATACAAA TGAAGCCGAG 
GATGTGGATA CACCCAGATA TAACAGAACA GACTCGAGAA ATGGAGTATT AAGAAGTATT 
AGCTTGGATT TATCATCTCC ACCTAATGTT GGCCTTCGCC GGAGTGGCCA AATTGAGGGT 
GTTCGCCAAA TGCATAGTAA TGCACCTCGG AGTCAAATTG CAACAGAAAG AGACCTAATG 
GCCTGGAGCA GAAGAGTGGT AGTAAATGAA CTGCATCCTG GTGTTATCAG GGTCCAGGAA 
GAATGCAGAA TTTCTAAAGG CGAAATGGAA ATCAGTTTGT ACAACACAGA GAAAAAGAGG 
AAACCAACTA ACCCTGCTCA GAGGAATGAT TATCAAGCTT TTGGTGGGCG ATCACTTAGA 
CGAATACAGC GGAAGCGCCA GCATGCCTAC CAGACACGTT CAACCATTGA ACATGATGCA 
AGAGCTAAAG TTCCAAGTAC AAATGGGGAA GATTCGCAGA GTTCCCCCGA GGAGGAGGAG 
TCCCAACATT CAACAGAGGC TTCAGGAGAT GAAGCTGCAG CTCAGTGGCA ATCAGAAACC 
TCTTCCAGTA ATTCATCTAG TGAATATTCC GACTGGACTG CAGATGCAGG AATCAATCTC 
CAGCCTCCCA AAAGACAGAC ACGGCAAACA ACAAGGAAAA TGTGCAGCAG TTCAGAGGAT 
GAGAACGCTA AAGAGGAAGA GAAGCCGAGG AAACACAAAC TGACCAGACG GAAGAAACAA 
AGTGAGCGTC TTTCTATGGA AGGAGAGCCA AGTGAAGAAT GGATTCCCCC CCAGTGGATC 
TTGGACACCA TCCCACGTCG TTCTCCTTTT GTTCCCCAGA TGGGAGATGA GGTTATTTAT 
TTCAGACAAG GCCATGAAGC TTATGTACGA GCAGTAAGGA AGGCTAAAAT ATATAATATT 
AATCCACAAA AGGAGCCATG GAACAAAACG GATCTCAGGG ATCAAGAATT TGCTAAGATT 
GTAGGAATTA AGTATGAGGT GGGGCCGCCT ACATTATGCT GCTTAAAACT TGCCTTACTG 
GATCCTACTT CAGGCAAAAT AACTGCAGAC TCTTTCTCTG TTAAATACCA TGACATGCCT 
GATGTTATTG ACTTCCTTGT CCTTTACCAG TTTTATAAGG AGGCGAAAGA GAGAAACTGG 
CAGATAGGAG ACCGGTTTCG AAGCATTATT GATGATGCAT GGTGGTTTGG CACAGTGGAA 
AGTCAGCAGC CATTCCAGAC TGAGTATCCT GACAGTTTAT TCCAGTGCTA CAGTGTACAC 
TGGGATAATA ATGAAAGAGA AAACATGAGT CCTTGGGACA TGGAACCAAT TCCAGATGGC 
ACAGCTTTGC CTGATGAAAT GGGTGCTGGT GTTCCCGTTA CTTCCGATGA ACTGAAGTCT 
TTGCAGTATA AACCGGAGGA GGGAGAGTGG GGATTGTACT CAAGAGATGA GGTTTGCGAA 
AGAGTTATCC AAGGAATTGA TCAGCTACTT ACTCTTGATT TTGCCAGCCC ATTCAGTGCT 
CCTGTGGATC TCAGTGCCTA CCCCTTATAC TGCACTGTGG TAGCATACCC AACAGATCTA 
AGCACCACAA GGAAGAGACT GGAGAGCCAC TACTATAGGA GGCTTTCTGC CCTAATATGG 
GAGGTCCGCT ATATTGAACA CAATGCAAGA ACATTCAATG AGCCTGACAC TCCAATTGTT 
AGAACTGCCA AAATTGTGAC AGATGTGCTG TTGCGTTATA TTGGGGATGA GAATTGTAAT 
GATATATTAG AGATCTACAA TCAAGTAAAG ACTGAAGATA CTGGCAATAC AGAAGATGAG 
GAGGGTGGAG CAGTTGATAT GGAATCAGAT GCCCCAGGGA CATCATCTGG TCGGGCTGTT 
ACCCGTCGCA CTAAAAGAGA TCTACTTCGT TCCAATCCTG ATTCATGGAA AGATAAATGC 
AAAGAACTAC TAGATTTAAT ATATGAGCGG GAAGATTCTG AACCCTTTAG GCAACCAGTC 
AATCAAGTCT CATATCCTGA TTATCAGGAT ATTATTGAAA CACCAATGGA TTTTAGCACC 
GTGAAAACAA CGTTAGAAGC TGGAAATTAT AGTGACCCGT TGGAATTTGC TGCAGATGTT 
CGACTAATCT TCAGTAACTC CAAAGCATAC ACTCCAAACA AAAAATCTAG AATCTACAGT 
ATGACTCTTC GACTCTCTGC ACTATGTGAA ACCTACATAA AGGATATCAT CTCTGACTAC 
AAACTAGCCA TGCAACTCCA ACAGAGAACA AGGCGGAAAT ACAGAAGGCG AATTAGGAGC 
AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC AGTAGTAATA GAAGAAGAAG TAGTAGTAGT 
AGTAGTAGTT CTAGTTCAGC ATCCAGCAGA GCATCCAGCC CAGACACAAA ACCTCGGAAA 
ATGAAGCTTC AACCGAAACT TAATCAGAAC CCTCCACATC CTCTTCCTAG GAGCAGCTCT 
TCATCCACTT CTCAAATGTC TGATACTGAT GTAGAGCCTG ATGGAAAGCC TTTTTCTGAA 
GATATGGATC AAGAGCCATC TTCATCTGGA TGCAGCAAAC CTGAAACACA CAAAGAACAG 
GAGAATGTGA CCAAGAGCAA AAATTCTCCT GTGAAAGAGG AACATACAAA TGGCCCTCTA 
ACAAATGGCG ATGGAGGAAA CACACCAAAA ACCAAGAGAA AGTTGCTAAG TTCTTCTGAG 
GATGAAAGTC CAGAAGATGG AGAGCAAATG GAAGAAAAGC CTGATAAAAG TCCAGCATGT 
TCTCGTGAAA GAGACTCTGA ATCAAGTTTA AGTTCGAAGA GTGAGTCAGA ATCAGAGAGC 
AGTTCTGATT CTGAATCCAT TAAGTCAGAT CAGGATTATA TTGATGGCGA CCATCATTAT 
AGCAAAGAGC CCCGTACAAA GTTTAAGAGA AAATTTTGTA AATCTGGAGA GGCTAAAACG 
AGAGGAAGGG GTCGAGGAAG AGGCAGAGGA AGAGGACGTG CAAAAGGTTA TAATCGTGGT 
GTGGAGGAAG AGTTAAAAAA AGGTAGGGGC CGGGGCAGAG GAAGAGGAAG AGGAGCATGG 
AAAAAAGAAC CTTCAAGGAC ACCGGCATTA AAACGTGCGC AATTGACAGA TGATGAAGAT 
GATGACAATG TGGATGATGA AGAAAGTGAT TGTGTAGAGC AACGAAGAAA AAAACCTAGG 
ATAAACACCA GGAACCAGGG CAGAAGAACT GTGTTATATA ATGATGAGTC TGATAATGAG 
CATGATGGTG CGGGGCAATA TGATGATCCC TTAAACCTGG GAACATCCAG GTCTGGAAGA 
GTGAGAAAAA TGACGGAAAA GGCCAGAGTG AGTCATCTGA TGGGATGGAA TGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002940482.3
CDS	335..5671
Translation	MEAELYFLIARFLQSGPCRRSAQVLAEELEEYRLIPPRLDWEGKEHRRTYQDLVAANKHIPSDYLLKICERFGPILDQEIPQSVPGLHSLLGLGKQSLLRSKKDCKNTPWKGTAFAALHRGRPPELPVNYGKPLNVVNITCARYQTGYAHYHRIFPSSTYQQMKMHKRILGHLSSVYCVAFDRTGRRIFTGSDDCLVKIWASDDGRLLSTLRGHSAEISDLAVNHENTLIAAGSCDKVVRVWCLRTCAPIAVLQGHTASITSIQFCPGTKEKTRYLTSTGADGTICFWEWHADTMTFNDRPVKFAERSRPGVQISCSSFSSGGMFIATGSTDHVIRIYYLGSGSPEKIAELEAHLDKVVAIQFCNNSDRLCFVSGSRDGTARIWQYQQQEWKMTVLDMTAKMAGSSVINTEDKVTKLKVTMVAWDRSDSSVITAVNNFFLKVWNSHTGELLHILSGHDDEVFVLEPHPFDPRILISAGHDGNIFVWDLETGTKIRNYFNMIEGQGHGAVFDCKFSPDGQNFACTDSHGHLLLFGFGCSKPYEKIPEQMFFHTDFRPLIRDSNSYVLDEQTQQAPHLMPPPFLVDVDGNPHPTRFQRLVPGRENCKDEQLIPQLGYMSNGNGDVVEQVIGQQTGNQEESILDGIIRELQREQDERHTNEAEDVDTPRYNRTDSRNGVLRSISLDLSSPPNVGLRRSGQIEGVRQMHSNAPRSQIATERDLMAWSRRVVVNELHPGVIRVQEECRISKGEMEISLYNTEKKRKPTNPAQRNDYQAFGGRSLRRIQRKRQHAYQTRSTIEHDARAKVPSTNGEDSQSSPEEEESQHSTEASGDEAAAQWQSETSSSNSSSEYSDWTADAGINLQPPKRQTRQTTRKMCSSSEDENAKEEEKPRKHKLTRRKKQSERLSMEGEPSEEWIPPQWILDTIPRRSPFVPQMGDEVIYFRQGHEAYVRAVRKAKIYNINPQKEPWNKTDLRDQEFAKIVGIKYEVGPPTLCCLKLALLDPTSGKITADSFSVKYHDMPDVIDFLVLYQFYKEAKERNWQIGDRFRSIIDDAWWFGTVESQQPFQTEYPDSLFQCYSVHWDNNERENMSPWDMEPIPDGTALPDEMGAGVPVTSDELKSLQYKPEEGEWGLYSRDEVCERVIQGIDQLLTLDFASPFSAPVDLSAYPLYCTVVAYPTDLSTTRKRLESHYYRRLSALIWEVRYIEHNARTFNEPDTPIVRTAKIVTDVLLRYIGDENCNDILEIYNQVKTEDTGNTEDEEGGAVDMESDAPGTSSGRAVTRRTKRDLLRSNPDSWKDKCKELLDLIYEREDSEPFRQPVNQVSYPDYQDIIETPMDFSTVKTTLEAGNYSDPLEFAADVRLIFSNSKAYTPNKKSRIYSMTLRLSALCETYIKDIISDYKLAMQLQQRTRRKYRRRIRSSSSSSSSSSSSSNRRRSSSSSSSSSSASSRASSPDTKPRKMKLQPKLNQNPPHPLPRSSSSSTSQMSDTDVEPDGKPFSEDMDQEPSSSGCSKPETHKEQENVTKSKNSPVKEEHTNGPLTNGDGGNTPKTKRKLLSSSEDESPEDGEQMEEKPDKSPACSRERDSESSLSSKSESESESSSDSESIKSDQDYIDGDHHYSKEPRTKFKRKFCKSGEAKTRGRGRGRGRGRGRAKGYNRGVEEELKKGRGRGRGRGRGAWKKEPSRTPALKRAQLTDDEDDDNVDDEESDCVEQRRKKPRINTRNQGRRTVLYNDESDNEHDGAGQYDDPLNLGTSRSGRVRKMTEKARVSHLMGWNG

Target ORF information:

RefSeq Version	XM_002940436.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis bromodomain and WD repeat domain containing 3 (brwd3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002940436.5

ORF Insert Sequence:

ATGGAAGCCG AGCTTTACTT CCTGATCGCC CGCTTCCTAC AGTCTGGCCC CTGCCGGAGA 
TCCGCACAGG TTCTGGCGGA GGAACTGGAG GAGTACCGGC TCATCCCACC GCGCTTAGAC 
TGGGAAGGGA AGGAACACCG CAGAACTTAT CAGGATTTGG TGGCAGCCAA TAAACACATC 
CCATCAGACT ACCTGCTTAA AATCTGCGAG CGTTTTGGAC CTATCCTAGA TCAAGAAATC 
CCGCAGAGTG TTCCTGGGTT GCATAGTCTG TTGGGACTTG GAAAACAGTC ACTGCTCAGA 
AGTAAAAAAG ACTGCAAGAA TACACCATGG AAAGGGACAG CTTTTGCAGC TTTGCACAGA 
GGGAGACCAC CAGAACTTCC AGTCAACTAT GGCAAGCCCC TCAACGTTGT AAATATCACT 
TGCGCCAGAT ATCAGACTGG ATATGCACAT TACCATCGCA TATTTCCATC TTCAACTTAT 
CAGCAGATGA AAATGCACAA GAGGATTTTG GGCCATCTTT CATCTGTATA CTGTGTGGCT 
TTTGATCGCA CTGGAAGACG TATTTTCACT GGTTCAGATG ACTGTCTAGT CAAAATATGG 
GCAAGTGATG ATGGACGTCT TCTTTCCACA CTACGTGGCC ATTCAGCTGA AATCTCTGAT 
CTGGCTGTGA ATCATGAGAA TACATTGATT GCTGCTGGCA GCTGTGATAA GGTGGTGCGT 
GTGTGGTGCC TTCGTACATG TGCACCAATA GCTGTGCTGC AGGGTCATAC AGCTTCTATC 
ACTTCCATAC AGTTTTGCCC AGGAACAAAA GAAAAAACAA GATACCTCAC ATCTACTGGT 
GCTGATGGGA CAATCTGCTT CTGGGAGTGG CATGCAGATA CCATGACATT TAATGATCGC 
CCTGTGAAAT TTGCAGAGAG GTCCAGGCCT GGGGTTCAGA TCTCCTGCTC ATCCTTTAGT 
TCAGGTGGTA TGTTTATAGC CACAGGAAGT ACTGATCATG TGATAAGAAT ATACTACCTG 
GGATCTGGTT CACCAGAGAA GATAGCTGAA TTGGAAGCAC ATTTGGACAA AGTGGTCGCT 
ATTCAGTTCT GCAACAATTC AGATCGATTG TGCTTTGTCA GTGGAAGCAG AGATGGCACT 
GCTCGGATTT GGCAATATCA GCAGCAAGAG TGGAAAATGA CTGTTCTGGA TATGACTGCC 
AAGATGGCTG GAAGCAGTGT TATAAATACA GAAGACAAAG TGACCAAGCT TAAAGTCACT 
ATGGTAGCAT GGGACCGATC TGATTCTTCG GTTATTACTG CTGTAAACAA TTTCTTTCTG 
AAAGTTTGGA ACTCTCATAC TGGCGAACTT CTTCATATAT TGTCTGGCCA TGATGATGAA 
GTTTTTGTTT TGGAACCTCA TCCTTTTGAT CCAAGGATTT TAATCTCTGC TGGGCATGAT 
GGAAACATAT TTGTGTGGGA CCTCGAAACA GGAACCAAAA TTCGTAATTA CTTTAACATG 
ATTGAAGGTC AGGGTCATGG AGCCGTGTTT GACTGTAAGT TCTCGCCTGA TGGTCAAAAC 
TTTGCCTGTA CAGATTCTCA TGGACATCTT TTACTCTTTG GTTTTGGTTG CAGTAAACCA 
TATGAAAAGA TTCCAGAACA GATGTTCTTC CATACAGACT TTAGACCTCT AATAAGAGAC 
TCCAACAGCT ATGTTTTGGA TGAACAAACT CAACAAGCCC CACACCTAAT GCCCCCTCCA 
TTCCTAGTAG ACGTTGATGG CAACCCACAT CCTACCCGTT TTCAGCGCTT GGTGCCAGGA 
CGTGAGAATT GTAAAGATGA GCAGCTCATA CCTCAGCTGG GATACATGTC AAATGGCAAT 
GGCGATGTGG TGGAGCAGGT CATTGGCCAG CAAACAGGAA ACCAGGAGGA AAGTATTCTT 
GATGGGATAA TCCGAGAACT CCAGAGGGAA CAGGATGAGA GGCATACAAA TGAAGCCGAG 
GATGTGGATA CACCCAGATA TAACAGAACA GACTCGAGAA ATGGAGTATT AAGAAGTATT 
AGCTTGGATT TATCATCTCC ACCTAATGTT GGCCTTCGCC GGAGTGGCCA AATTGAGGGT 
GTTCGCCAAA TGCATAGTAA TGCACCTCGG AGTCAAATTG CAACAGAAAG AGACCTAATG 
GCCTGGAGCA GAAGAGTGGT AGTAAATGAA CTGCATCCTG GTGTTATCAG GGTCCAGGAA 
GAATGCAGAA TTTCTAAAGG CGAAATGGAA ATCAGTTTGT ACAACACAGA GAAAAAGAGG 
AAACCAACTA ACCCTGCTCA GAGGAATGAT TATCAAGCTT TTGGTGGGCG ATCACTTAGA 
CGAATACAGC GGAAGCGCCA GCATGCCTAC CAGACACGTT CAACCATTGA ACATGATGCA 
AGAGCTAAAG TTCCAAGTAC AAATGGGGAA GATTCGCAGA GTTCCCCCGA GGAGGAGGAG 
TCCCAACATT CAACAGAGGC TTCAGGAGAT GAAGCTGCAG CTCAGTGGCA ATCAGAAACC 
TCTTCCAGTA ATTCATCTAG TGAATATTCC GACTGGACTG CAGATGCAGG AATCAATCTC 
CAGCCTCCCA AAAGACAGAC ACGGCAAACA ACAAGGAAAA TGTGCAGCAG TTCAGAGGAT 
GAGAACGCTA AAGAGGAAGA GAAGCCGAGG AAACACAAAC TGACCAGACG GAAGAAACAA 
AGTGAGCGTC TTTCTATGGA AGGAGAGCCA AGTGAAGAAT GGATTCCCCC CCAGTGGATC 
TTGGACACCA TCCCACGTCG TTCTCCTTTT GTTCCCCAGA TGGGAGATGA GGTTATTTAT 
TTCAGACAAG GCCATGAAGC TTATGTACGA GCAGTAAGGA AGGCTAAAAT ATATAATATT 
AATCCACAAA AGGAGCCATG GAACAAAACG GATCTCAGGG ATCAAGAATT TGCTAAGATT 
GTAGGAATTA AGTATGAGGT GGGGCCGCCT ACATTATGCT GCTTAAAACT TGCCTTACTG 
GATCCTACTT CAGGCAAAAT AACTGCAGAC TCTTTCTCTG TTAAATACCA TGACATGCCT 
GATGTTATTG ACTTCCTTGT CCTTTACCAG TTTTATAAGG AGGCGAAAGA GAGAAACTGG 
CAGATAGGAG ACCGGTTTCG AAGCATTATT GATGATGCAT GGTGGTTTGG CACAGTGGAA 
AGTCAGCAGC CATTCCAGAC TGAGTATCCT GACAGTTTAT TCCAGTGCTA CAGTGTACAC 
TGGGATAATA ATGAAAGAGA AAACATGAGT CCTTGGGACA TGGAACCAAT TCCAGATGGC 
ACAGCTTTGC CTGATGAAAT GGGTGCTGGT GTTCCCGTTA CTTCCGATGA ACTGAAGTCT 
TTGCAGTATA AACCGGAGGA GGGAGAGTGG GGATTGTACT CAAGAGATGA GGTTTGCGAA 
AGAGTTATCC AAGGAATTGA TCAGCTACTT ACTCTTGATT TTGCCAGCCC ATTCAGTGCT 
CCTGTGGATC TCAGTGCCTA CCCCTTATAC TGCACTGTGG TAGCATACCC AACAGATCTA 
AGCACCACAA GGAAGAGACT GGAGAGCCAC TACTATAGGA GGCTTTCTGC CCTAATATGG 
GAGGTCCGCT ATATTGAACA CAATGCAAGA ACATTCAATG AGCCTGACAC TCCAATTGTT 
AGAACTGCCA AAATTGTGAC AGATGTGCTG TTGCGTTATA TTGGGGATGA GAATTGTAAT 
GATATATTAG AGATCTACAA TCAAGTAAAG ACTGAAGATA CTGGCAATAC AGAAGATGAG 
GAGGGTGGAG CAGTTGATAT GGAATCAGAT GCCCCAGGGA CATCATCTGG TCGGGCTGTT 
ACCCGTCGCA CTAAAAGAGA TCTACTTCGT TCCAATCCTG ATTCATGGAA AGATAAATGC 
AAAGAACTAC TAGATTTAAT ATATGAGCGG GAAGATTCTG AACCCTTTAG GCAACCAGTC 
AATCAAGTCT CATATCCTGA TTATCAGGAT ATTATTGAAA CACCAATGGA TTTTAGCACC 
GTGAAAACAA CGTTAGAAGC TGGAAATTAT AGTGACCCGT TGGAATTTGC TGCAGATGTT 
CGACTAATCT TCAGTAACTC CAAAGCATAC ACTCCAAACA AAAAATCTAG AATCTACAGT 
ATGACTCTTC GACTCTCTGC ACTATGTGAA ACCTACATAA AGGATATCAT CTCTGACTAC 
AAACTAGCCA TGCAACTCCA ACAGAGAACA AGGCGGAAAT ACAGAAGGCG AATTAGGAGC 
AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC AGTAGTAATA GAAGAAGAAG TAGTAGTAGT 
AGTAGTAGTT CTAGTTCAGC ATCCAGCAGA GCATCCAGCC CAGACACAAA ACCTCGGAAA 
ATGAAGCTTC AACCGAAACT TAATCAGAAC CCTCCACATC CTCTTCCTAG GAGCAGCTCT 
TCATCCACTT CTCAAATGTC TGATACTGAT GTAGAGCCTG ATGGAAAGCC TTTTTCTGAA 
GATATGGATC AAGAGCCATC TTCATCTGGA TGCAGCAAAC CTGAAACACA CAAAGAACAG 
GAGAATGTGA CCAAGAGCAA AAATTCTCCT GTGAAAGAGG AACATACAAA TGGCCCTCTA 
ACAAATGGCG ATGGAGGAAA CACACCAAAA ACCAAGAGAA AGTTGCTAAG TTCTTCTGAG 
GATGAAAGTC CAGAAGATGG AGAGCAAATG GAAGAAAAGC CTGATAAAAG TCCAGCATGT 
TCTCGTGAAA GAGACTCTGA ATCAAGTTTA AGTTCGAAGA GTGAGTCAGA ATCAGAGAGC 
AGTTCTGATT CTGAATCCAT TAAGTCAGAT CAGGATTATA TTGATGGCGA CCATCATTAT 
AGCAAAGAGC CCCGTACAAA GTTTAAGAGA AAATTTTGTA AATCTGGAGA GGCTAAAACG 
AGAGGAAGGG GTCGAGGAAG AGGCAGAGGA AGAGGACGTG CAAAAGGTTA TAATCGTGGT 
GTGGAGGAAG AGTTAAAAAA AGGTAGGGGC CGGGGCAGAG GAAGAGGAAG AGGAGCATGG 
AAAAAAGAAC CTTCAAGGAC ACCGGCATTA AAACGTGCGC AATTGACAGA TGATGAAGAT 
GATGACAATG TGGATGATGA AGAAAGTGAT TGTGTAGAGC AACGAAGAAA AAAACCTAGG 
ATAAACACCA GGAACCAGGG CAGAAGAACT GTGTTATATA ATGATGAGTC TGATAATGAG 
CATGATGGTG CGGGGCAATA TGATGATCCC TTAAACCTGG GAACATCCAG GTCTGGAAGA 
GTGAGAAAAA TGACGGAAAA GGCCAGAGTG AGTCATCTGA TGGGATGGAA TGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

brwd3 ( XM_004916894.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004916951.2 Xenopus tropicalis bromodomain and WD repeat domain containing 3 (brwd3), transcript variant X2, mRNA.

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CloneID	OXa64564
Clone ID Related Accession (Same CDS sequence)	XM_004916894.4
Accession Version	XM_004916894.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5334bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	bromodomain and WD repeat-containing protein 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004916894.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCCG AGCTTTACTT CCTGATCGCC CGCTTCCTAC AGTCTGGCCC CTGCCGGAGA 
TCCGCACAGG TTCTGGCGGA GGAACTGGAG GAGTACCGGC TCATCCCACC GCGCTTAGAC 
TGGGAAGGGA AGGAACACCG CAGAACTTAT CAGGATTTGG TGGCAGCCAA TAAACACATC 
CCATCAGACT ACCTGCTTAA AATCTGCGAG CGTTTTGGAC CTATCCTAGA TCAAGAAATC 
CCGCAGAGTG TTCCTGGGTT GCATAGTCTG TTGGGACTTG GAAAACAGTC ACTGCTCAGA 
AGTAAAAAAG ACTGCAAGAA TACACCATGG AAAGGGACAG CTTTTGCAGC TTTGCACAGA 
GGGAGACCAC CAGAACTTCC AGTCAACTAT GGCAAGCCCC TCAACGTTGT AAATATCACT 
TGCGCCAGAT ATCAGACTGG ATATGCACAT TACCATCGCA TATTTCCATC TTCAACTTAT 
CAGCAGATGA AAATGCACAA GAGGATTTTG GGCCATCTTT CATCTGTATA CTGTGTGGCT 
TTTGATCGCA CTGGAAGACG TATTTTCACT GGTTCAGATG ACTGTCTAGT CAAAATATGG 
GCAAGTGATG ATGGACGTCT TCTTTCCACA CTACGTGGCC ATTCAGCTGA AATCTCTGAT 
CTGGCTGTGA ATCATGAGAA TACATTGATT GCTGCTGGCA GCTGTGATAA GGTGGTGCGT 
GTGTGGTGCC TTCGTACATG TGCACCAATA GCTGTGCTGC AGGGTCATAC AGCTTCTATC 
ACTTCCATAC AGTTTTGCCC AGGAACAAAA GAAAAAACAA GATACCTCAC ATCTACTGGT 
GCTGATGGGA CAATCTGCTT CTGGGAGTGG CATGCAGATA CCATGACATT TAATGATCGC 
CCTGTGAAAT TTGCAGAGAG GTCCAGGCCT GGGGTTCAGA TCTCCTGCTC ATCCTTTAGT 
TCAGGTGGTA TGTTTATAGC CACAGGAAGT ACTGATCATG TGATAAGAAT ATACTACCTG 
GGATCTGGTT CACCAGAGAA GATAGCTGAA TTGGAAGCAC ATTTGGACAA AGTGGTCGCT 
ATTCAGTTCT GCAACAATTC AGATCGATTG TGCTTTGTCA GTGGAAGCAG AGATGGCACT 
GCTCGGATTT GGCAATATCA GCAGCAAGAG TGGAAAATGA CTGTTCTGGA TATGACTGCC 
AAGATGGCTG GAAGCAGTGT TATAAATACA GAAGACAAAG TGACCAAGCT TAAAGTCACT 
ATGGTAGCAT GGGACCGATC TGATTCTTCG GTTATTACTG CTGTAAACAA TTTCTTTCTG 
AAAGTTTGGA ACTCTCATAC TGGCGAACTT CTTCATATAT TGTCTGGCCA TGATGATGAA 
GTTTTTGTTT TGGAACCTCA TCCTTTTGAT CCAAGGATTT TAATCTCTGC TGGGCATGAT 
GGAAACATAT TTGTGTGGGA CCTCGAAACA GGAACCAAAA TTCGTAATTA CTTTAACATG 
ATTGAAGGTC AGGGTCATGG AGCCGTGTTT GACTGTAAGT TCTCGCCTGA TGGTCAAAAC 
TTTGCCTGTA CAGATTCTCA TGGACATCTT TTACTCTTTG GTTTTGGTTG CAGTAAACCA 
TATGAAAAGA TTCCAGAACA GATGTTCTTC CATACAGACT TTAGACCTCT AATAAGAGAC 
TCCAACAGCT ATGTTTTGGA TGAACAAACT CAACAAGCCC CACACCTAAT GCCCCCTCCA 
TTCCTAGTAG ACGTTGATGG CAACCCACAT CCTACCCGTT TTCAGCGCTT GGTGCCAGGA 
CGTGAGAATT GTAAAGATGA GCAGCTCATA CCTCAGCTGG GATACATGTC AAATGGCAAT 
GGCGATGTGG TGGAGCAGGT CATTGGCCAG CAAACAGGAA ACCAGGAGGA AAGTATTCTT 
GATGGGATAA TCCGAGAACT CCAGAGGGAA CAGGATGAGA GGCATACAAA TGAAGCCGAG 
GATGTGGATA CACCCAGATA TAACAGAACA GACTCGAGAA ATGGAGTATT AAGAAGTATT 
AGCTTGGATT TATCATCTCC ACCTAATGTT GGCCTTCGCC GGAGTGGCCA AATTGAGGGT 
GTTCGCCAAA TGCATAGTAA TGCACCTCGG AGTCAAATTG CAACAGAAAG AGACCTAATG 
GCCTGGAGCA GAAGAGTGGT AGTAAATGAA CTGCATCCTG GTGTTATCAG GGTCCAGGAA 
GAATGCAGAA TTTCTAAAGG CGAAATGGAA ATCAGTTTGT ACAACACAGA GAAAAAGAGG 
AAACCAACTA ACCCTGCTCA GAGGAATGAT TATCAAGCTT TTGGTGGGCG ATCACTTAGA 
CGAATACAGC GGAAGCGCCA GCATGCCTAC CAGACACGTT CAACCATTGA ACATGATGCA 
AGAGCTAAAG TTCCAAGTAC AAATGGGGAA GATTCGCAGA GTTCCCCCGA GGAGGAGGAG 
TCCCAACATT CAACAGAGGC TTCAGGAGAT GAAGCTGCAG CTCAGTGGCA ATCAGAAACC 
TCTTCCAGTA ATTCATCTAG TGAATATTCC GACTGGACTG CAGATGCAGG AATCAATCTC 
CAGCCTCCCA AAAGACAGAC ACGGCAAACA ACAAGGAAAA TGTGCAGCAG TTCAGAGGAT 
GAGAACGCTA AAGAGGAAGA GAAGCCGAGG AAACACAAAC TGACCAGACG GAAGAAACAA 
AGTGAGCGTC TTTCTATGGA AGGAGAGCCA AGTGAAGAAT GGATTCCCCC CCAGTGGATC 
TTGGACACCA TCCCACGTCG TTCTCCTTTT GTTCCCCAGA TGGGAGATGA GGTTATTTAT 
TTCAGACAAG GCCATGAAGC TTATGTACGA GCAGTAAGGA AGGCTAAAAT ATATAATATT 
AATCCACAAA AGGAGCCATG GAACAAAACG GATCTCAGGG ATCAAGAATT TGCTAAGATT 
GTAGGAATTA AGTATGAGGT GGGGCCGCCT ACATTATGCT GCTTAAAACT TGCCTTACTG 
GATCCTACTT CAGGCAAAAT AACTGCAGAC TCTTTCTCTG TTAAATACCA TGACATGCCT 
GATGTTATTG ACTTCCTTGT CCTTTACCAG TTTTATAAGG AGGCGAAAGA GAGAAACTGG 
CAGATAGGAG ACCGGTTTCG AAGCATTATT GATGATGCAT GGTGGTTTGG CACAGTGGAA 
AGTCAGCAGC CATTCCAGAC TGAGTATCCT GACAGTTTAT TCCAGTGCTA CAGTGTACAC 
TGGGATAATA ATGAAAGAGA AAACATGAGT CCTTGGGACA TGGAACCAAT TCCAGATGGC 
ACTTTGCCTG ATGAAATGGG TGCTGGTGTT CCCGTTACTT CCGATGAACT GAAGTCTTTG 
CAGTATAAAC CGGAGGAGGG AGAGTGGGGA TTGTACTCAA GAGATGAGGT TTGCGAAAGA 
GTTATCCAAG GAATTGATCA GCTACTTACT CTTGATTTTG CCAGCCCATT CAGTGCTCCT 
GTGGATCTCA GTGCCTACCC CTTATACTGC ACTGTGGTAG CATACCCAAC AGATCTAAGC 
ACCACAAGGA AGAGACTGGA GAGCCACTAC TATAGGAGGC TTTCTGCCCT AATATGGGAG 
GTCCGCTATA TTGAACACAA TGCAAGAACA TTCAATGAGC CTGACACTCC AATTGTTAGA 
ACTGCCAAAA TTGTGACAGA TGTGCTGTTG CGTTATATTG GGGATGAGAA TTGTAATGAT 
ATATTAGAGA TCTACAATCA AGTAAAGACT GAAGATACTG GCAATACAGA AGATGAGGAG 
GGTGGAGCAG TTGATATGGA ATCAGATGCC CCAGGGACAT CATCTGGTCG GGCTGTTACC 
CGTCGCACTA AAAGAGATCT ACTTCGTTCC AATCCTGATT CATGGAAAGA TAAATGCAAA 
GAACTACTAG ATTTAATATA TGAGCGGGAA GATTCTGAAC CCTTTAGGCA ACCAGTCAAT 
CAAGTCTCAT ATCCTGATTA TCAGGATATT ATTGAAACAC CAATGGATTT TAGCACCGTG 
AAAACAACGT TAGAAGCTGG AAATTATAGT GACCCGTTGG AATTTGCTGC AGATGTTCGA 
CTAATCTTCA GTAACTCCAA AGCATACACT CCAAACAAAA AATCTAGAAT CTACAGTATG 
ACTCTTCGAC TCTCTGCACT ATGTGAAACC TACATAAAGG ATATCATCTC TGACTACAAA 
CTAGCCATGC AACTCCAACA GAGAACAAGG CGGAAATACA GAAGGCGAAT TAGGAGCAGC 
AGCAGCAGCA GCAGCAGCAG CAGCAGCAGT AGTAATAGAA GAAGAAGTAG TAGTAGTAGT 
AGTAGTTCTA GTTCAGCATC CAGCAGAGCA TCCAGCCCAG ACACAAAACC TCGGAAAATG 
AAGCTTCAAC CGAAACTTAA TCAGAACCCT CCACATCCTC TTCCTAGGAG CAGCTCTTCA 
TCCACTTCTC AAATGTCTGA TACTGATGTA GAGCCTGATG GAAAGCCTTT TTCTGAAGAT 
ATGGATCAAG AGCCATCTTC ATCTGGATGC AGCAAACCTG AAACACACAA AGAACAGGAG 
AATGTGACCA AGAGCAAAAA TTCTCCTGTG AAAGAGGAAC ATACAAATGG CCCTCTAACA 
AATGGCGATG GAGGAAACAC ACCAAAAACC AAGAGAAAGT TGCTAAGTTC TTCTGAGGAT 
GAAAGTCCAG AAGATGGAGA GCAAATGGAA GAAAAGCCTG ATAAAAGTCC AGCATGTTCT 
CGTGAAAGAG ACTCTGAATC AAGTTTAAGT TCGAAGAGTG AGTCAGAATC AGAGAGCAGT 
TCTGATTCTG AATCCATTAA GTCAGATCAG GATTATATTG ATGGCGACCA TCATTATAGC 
AAAGAGCCCC GTACAAAGTT TAAGAGAAAA TTTTGTAAAT CTGGAGAGGC TAAAACGAGA 
GGAAGGGGTC GAGGAAGAGG CAGAGGAAGA GGACGTGCAA AAGGTTATAA TCGTGGTGTG 
GAGGAAGAGT TAAAAAAAGG TAGGGGCCGG GGCAGAGGAA GAGGAAGAGG AGCATGGAAA 
AAAGAACCTT CAAGGACACC GGCATTAAAA CGTGCGCAAT TGACAGATGA TGAAGATGAT 
GACAATGTGG ATGATGAAGA AAGTGATTGT GTAGAGCAAC GAAGAAAAAA ACCTAGGATA 
AACACCAGGA ACCAGGGCAG AAGAACTGTG TTATATAATG ATGAGTCTGA TAATGAGCAT 
GATGGTGCGG GGCAATATGA TGATCCCTTA AACCTGGGAA CATCCAGGTC TGGAAGAGTG 
AGAAAAATGA CGGAAAAGGC CAGAGTGAGT CATCTGATGG GATGGAATGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004916951.2
CDS	335..5668
Translation	MEAELYFLIARFLQSGPCRRSAQVLAEELEEYRLIPPRLDWEGKEHRRTYQDLVAANKHIPSDYLLKICERFGPILDQEIPQSVPGLHSLLGLGKQSLLRSKKDCKNTPWKGTAFAALHRGRPPELPVNYGKPLNVVNITCARYQTGYAHYHRIFPSSTYQQMKMHKRILGHLSSVYCVAFDRTGRRIFTGSDDCLVKIWASDDGRLLSTLRGHSAEISDLAVNHENTLIAAGSCDKVVRVWCLRTCAPIAVLQGHTASITSIQFCPGTKEKTRYLTSTGADGTICFWEWHADTMTFNDRPVKFAERSRPGVQISCSSFSSGGMFIATGSTDHVIRIYYLGSGSPEKIAELEAHLDKVVAIQFCNNSDRLCFVSGSRDGTARIWQYQQQEWKMTVLDMTAKMAGSSVINTEDKVTKLKVTMVAWDRSDSSVITAVNNFFLKVWNSHTGELLHILSGHDDEVFVLEPHPFDPRILISAGHDGNIFVWDLETGTKIRNYFNMIEGQGHGAVFDCKFSPDGQNFACTDSHGHLLLFGFGCSKPYEKIPEQMFFHTDFRPLIRDSNSYVLDEQTQQAPHLMPPPFLVDVDGNPHPTRFQRLVPGRENCKDEQLIPQLGYMSNGNGDVVEQVIGQQTGNQEESILDGIIRELQREQDERHTNEAEDVDTPRYNRTDSRNGVLRSISLDLSSPPNVGLRRSGQIEGVRQMHSNAPRSQIATERDLMAWSRRVVVNELHPGVIRVQEECRISKGEMEISLYNTEKKRKPTNPAQRNDYQAFGGRSLRRIQRKRQHAYQTRSTIEHDARAKVPSTNGEDSQSSPEEEESQHSTEASGDEAAAQWQSETSSSNSSSEYSDWTADAGINLQPPKRQTRQTTRKMCSSSEDENAKEEEKPRKHKLTRRKKQSERLSMEGEPSEEWIPPQWILDTIPRRSPFVPQMGDEVIYFRQGHEAYVRAVRKAKIYNINPQKEPWNKTDLRDQEFAKIVGIKYEVGPPTLCCLKLALLDPTSGKITADSFSVKYHDMPDVIDFLVLYQFYKEAKERNWQIGDRFRSIIDDAWWFGTVESQQPFQTEYPDSLFQCYSVHWDNNERENMSPWDMEPIPDGTLPDEMGAGVPVTSDELKSLQYKPEEGEWGLYSRDEVCERVIQGIDQLLTLDFASPFSAPVDLSAYPLYCTVVAYPTDLSTTRKRLESHYYRRLSALIWEVRYIEHNARTFNEPDTPIVRTAKIVTDVLLRYIGDENCNDILEIYNQVKTEDTGNTEDEEGGAVDMESDAPGTSSGRAVTRRTKRDLLRSNPDSWKDKCKELLDLIYEREDSEPFRQPVNQVSYPDYQDIIETPMDFSTVKTTLEAGNYSDPLEFAADVRLIFSNSKAYTPNKKSRIYSMTLRLSALCETYIKDIISDYKLAMQLQQRTRRKYRRRIRSSSSSSSSSSSSSNRRRSSSSSSSSSSASSRASSPDTKPRKMKLQPKLNQNPPHPLPRSSSSSTSQMSDTDVEPDGKPFSEDMDQEPSSSGCSKPETHKEQENVTKSKNSPVKEEHTNGPLTNGDGGNTPKTKRKLLSSSEDESPEDGEQMEEKPDKSPACSRERDSESSLSSKSESESESSSDSESIKSDQDYIDGDHHYSKEPRTKFKRKFCKSGEAKTRGRGRGRGRGRGRAKGYNRGVEEELKKGRGRGRGRGRGAWKKEPSRTPALKRAQLTDDEDDDNVDDEESDCVEQRRKKPRINTRNQGRRTVLYNDESDNEHDGAGQYDDPLNLGTSRSGRVRKMTEKARVSHLMGWNG

Target ORF information:

RefSeq Version	XM_004916894.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis bromodomain and WD repeat domain containing 3 (brwd3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916894.4

ORF Insert Sequence:

ATGGAAGCCG AGCTTTACTT CCTGATCGCC CGCTTCCTAC AGTCTGGCCC CTGCCGGAGA 
TCCGCACAGG TTCTGGCGGA GGAACTGGAG GAGTACCGGC TCATCCCACC GCGCTTAGAC 
TGGGAAGGGA AGGAACACCG CAGAACTTAT CAGGATTTGG TGGCAGCCAA TAAACACATC 
CCATCAGACT ACCTGCTTAA AATCTGCGAG CGTTTTGGAC CTATCCTAGA TCAAGAAATC 
CCGCAGAGTG TTCCTGGGTT GCATAGTCTG TTGGGACTTG GAAAACAGTC ACTGCTCAGA 
AGTAAAAAAG ACTGCAAGAA TACACCATGG AAAGGGACAG CTTTTGCAGC TTTGCACAGA 
GGGAGACCAC CAGAACTTCC AGTCAACTAT GGCAAGCCCC TCAACGTTGT AAATATCACT 
TGCGCCAGAT ATCAGACTGG ATATGCACAT TACCATCGCA TATTTCCATC TTCAACTTAT 
CAGCAGATGA AAATGCACAA GAGGATTTTG GGCCATCTTT CATCTGTATA CTGTGTGGCT 
TTTGATCGCA CTGGAAGACG TATTTTCACT GGTTCAGATG ACTGTCTAGT CAAAATATGG 
GCAAGTGATG ATGGACGTCT TCTTTCCACA CTACGTGGCC ATTCAGCTGA AATCTCTGAT 
CTGGCTGTGA ATCATGAGAA TACATTGATT GCTGCTGGCA GCTGTGATAA GGTGGTGCGT 
GTGTGGTGCC TTCGTACATG TGCACCAATA GCTGTGCTGC AGGGTCATAC AGCTTCTATC 
ACTTCCATAC AGTTTTGCCC AGGAACAAAA GAAAAAACAA GATACCTCAC ATCTACTGGT 
GCTGATGGGA CAATCTGCTT CTGGGAGTGG CATGCAGATA CCATGACATT TAATGATCGC 
CCTGTGAAAT TTGCAGAGAG GTCCAGGCCT GGGGTTCAGA TCTCCTGCTC ATCCTTTAGT 
TCAGGTGGTA TGTTTATAGC CACAGGAAGT ACTGATCATG TGATAAGAAT ATACTACCTG 
GGATCTGGTT CACCAGAGAA GATAGCTGAA TTGGAAGCAC ATTTGGACAA AGTGGTCGCT 
ATTCAGTTCT GCAACAATTC AGATCGATTG TGCTTTGTCA GTGGAAGCAG AGATGGCACT 
GCTCGGATTT GGCAATATCA GCAGCAAGAG TGGAAAATGA CTGTTCTGGA TATGACTGCC 
AAGATGGCTG GAAGCAGTGT TATAAATACA GAAGACAAAG TGACCAAGCT TAAAGTCACT 
ATGGTAGCAT GGGACCGATC TGATTCTTCG GTTATTACTG CTGTAAACAA TTTCTTTCTG 
AAAGTTTGGA ACTCTCATAC TGGCGAACTT CTTCATATAT TGTCTGGCCA TGATGATGAA 
GTTTTTGTTT TGGAACCTCA TCCTTTTGAT CCAAGGATTT TAATCTCTGC TGGGCATGAT 
GGAAACATAT TTGTGTGGGA CCTCGAAACA GGAACCAAAA TTCGTAATTA CTTTAACATG 
ATTGAAGGTC AGGGTCATGG AGCCGTGTTT GACTGTAAGT TCTCGCCTGA TGGTCAAAAC 
TTTGCCTGTA CAGATTCTCA TGGACATCTT TTACTCTTTG GTTTTGGTTG CAGTAAACCA 
TATGAAAAGA TTCCAGAACA GATGTTCTTC CATACAGACT TTAGACCTCT AATAAGAGAC 
TCCAACAGCT ATGTTTTGGA TGAACAAACT CAACAAGCCC CACACCTAAT GCCCCCTCCA 
TTCCTAGTAG ACGTTGATGG CAACCCACAT CCTACCCGTT TTCAGCGCTT GGTGCCAGGA 
CGTGAGAATT GTAAAGATGA GCAGCTCATA CCTCAGCTGG GATACATGTC AAATGGCAAT 
GGCGATGTGG TGGAGCAGGT CATTGGCCAG CAAACAGGAA ACCAGGAGGA AAGTATTCTT 
GATGGGATAA TCCGAGAACT CCAGAGGGAA CAGGATGAGA GGCATACAAA TGAAGCCGAG 
GATGTGGATA CACCCAGATA TAACAGAACA GACTCGAGAA ATGGAGTATT AAGAAGTATT 
AGCTTGGATT TATCATCTCC ACCTAATGTT GGCCTTCGCC GGAGTGGCCA AATTGAGGGT 
GTTCGCCAAA TGCATAGTAA TGCACCTCGG AGTCAAATTG CAACAGAAAG AGACCTAATG 
GCCTGGAGCA GAAGAGTGGT AGTAAATGAA CTGCATCCTG GTGTTATCAG GGTCCAGGAA 
GAATGCAGAA TTTCTAAAGG CGAAATGGAA ATCAGTTTGT ACAACACAGA GAAAAAGAGG 
AAACCAACTA ACCCTGCTCA GAGGAATGAT TATCAAGCTT TTGGTGGGCG ATCACTTAGA 
CGAATACAGC GGAAGCGCCA GCATGCCTAC CAGACACGTT CAACCATTGA ACATGATGCA 
AGAGCTAAAG TTCCAAGTAC AAATGGGGAA GATTCGCAGA GTTCCCCCGA GGAGGAGGAG 
TCCCAACATT CAACAGAGGC TTCAGGAGAT GAAGCTGCAG CTCAGTGGCA ATCAGAAACC 
TCTTCCAGTA ATTCATCTAG TGAATATTCC GACTGGACTG CAGATGCAGG AATCAATCTC 
CAGCCTCCCA AAAGACAGAC ACGGCAAACA ACAAGGAAAA TGTGCAGCAG TTCAGAGGAT 
GAGAACGCTA AAGAGGAAGA GAAGCCGAGG AAACACAAAC TGACCAGACG GAAGAAACAA 
AGTGAGCGTC TTTCTATGGA AGGAGAGCCA AGTGAAGAAT GGATTCCCCC CCAGTGGATC 
TTGGACACCA TCCCACGTCG TTCTCCTTTT GTTCCCCAGA TGGGAGATGA GGTTATTTAT 
TTCAGACAAG GCCATGAAGC TTATGTACGA GCAGTAAGGA AGGCTAAAAT ATATAATATT 
AATCCACAAA AGGAGCCATG GAACAAAACG GATCTCAGGG ATCAAGAATT TGCTAAGATT 
GTAGGAATTA AGTATGAGGT GGGGCCGCCT ACATTATGCT GCTTAAAACT TGCCTTACTG 
GATCCTACTT CAGGCAAAAT AACTGCAGAC TCTTTCTCTG TTAAATACCA TGACATGCCT 
GATGTTATTG ACTTCCTTGT CCTTTACCAG TTTTATAAGG AGGCGAAAGA GAGAAACTGG 
CAGATAGGAG ACCGGTTTCG AAGCATTATT GATGATGCAT GGTGGTTTGG CACAGTGGAA 
AGTCAGCAGC CATTCCAGAC TGAGTATCCT GACAGTTTAT TCCAGTGCTA CAGTGTACAC 
TGGGATAATA ATGAAAGAGA AAACATGAGT CCTTGGGACA TGGAACCAAT TCCAGATGGC 
ACTTTGCCTG ATGAAATGGG TGCTGGTGTT CCCGTTACTT CCGATGAACT GAAGTCTTTG 
CAGTATAAAC CGGAGGAGGG AGAGTGGGGA TTGTACTCAA GAGATGAGGT TTGCGAAAGA 
GTTATCCAAG GAATTGATCA GCTACTTACT CTTGATTTTG CCAGCCCATT CAGTGCTCCT 
GTGGATCTCA GTGCCTACCC CTTATACTGC ACTGTGGTAG CATACCCAAC AGATCTAAGC 
ACCACAAGGA AGAGACTGGA GAGCCACTAC TATAGGAGGC TTTCTGCCCT AATATGGGAG 
GTCCGCTATA TTGAACACAA TGCAAGAACA TTCAATGAGC CTGACACTCC AATTGTTAGA 
ACTGCCAAAA TTGTGACAGA TGTGCTGTTG CGTTATATTG GGGATGAGAA TTGTAATGAT 
ATATTAGAGA TCTACAATCA AGTAAAGACT GAAGATACTG GCAATACAGA AGATGAGGAG 
GGTGGAGCAG TTGATATGGA ATCAGATGCC CCAGGGACAT CATCTGGTCG GGCTGTTACC 
CGTCGCACTA AAAGAGATCT ACTTCGTTCC AATCCTGATT CATGGAAAGA TAAATGCAAA 
GAACTACTAG ATTTAATATA TGAGCGGGAA GATTCTGAAC CCTTTAGGCA ACCAGTCAAT 
CAAGTCTCAT ATCCTGATTA TCAGGATATT ATTGAAACAC CAATGGATTT TAGCACCGTG 
AAAACAACGT TAGAAGCTGG AAATTATAGT GACCCGTTGG AATTTGCTGC AGATGTTCGA 
CTAATCTTCA GTAACTCCAA AGCATACACT CCAAACAAAA AATCTAGAAT CTACAGTATG 
ACTCTTCGAC TCTCTGCACT ATGTGAAACC TACATAAAGG ATATCATCTC TGACTACAAA 
CTAGCCATGC AACTCCAACA GAGAACAAGG CGGAAATACA GAAGGCGAAT TAGGAGCAGC 
AGCAGCAGCA GCAGCAGCAG CAGCAGCAGT AGTAATAGAA GAAGAAGTAG TAGTAGTAGT 
AGTAGTTCTA GTTCAGCATC CAGCAGAGCA TCCAGCCCAG ACACAAAACC TCGGAAAATG 
AAGCTTCAAC CGAAACTTAA TCAGAACCCT CCACATCCTC TTCCTAGGAG CAGCTCTTCA 
TCCACTTCTC AAATGTCTGA TACTGATGTA GAGCCTGATG GAAAGCCTTT TTCTGAAGAT 
ATGGATCAAG AGCCATCTTC ATCTGGATGC AGCAAACCTG AAACACACAA AGAACAGGAG 
AATGTGACCA AGAGCAAAAA TTCTCCTGTG AAAGAGGAAC ATACAAATGG CCCTCTAACA 
AATGGCGATG GAGGAAACAC ACCAAAAACC AAGAGAAAGT TGCTAAGTTC TTCTGAGGAT 
GAAAGTCCAG AAGATGGAGA GCAAATGGAA GAAAAGCCTG ATAAAAGTCC AGCATGTTCT 
CGTGAAAGAG ACTCTGAATC AAGTTTAAGT TCGAAGAGTG AGTCAGAATC AGAGAGCAGT 
TCTGATTCTG AATCCATTAA GTCAGATCAG GATTATATTG ATGGCGACCA TCATTATAGC 
AAAGAGCCCC GTACAAAGTT TAAGAGAAAA TTTTGTAAAT CTGGAGAGGC TAAAACGAGA 
GGAAGGGGTC GAGGAAGAGG CAGAGGAAGA GGACGTGCAA AAGGTTATAA TCGTGGTGTG 
GAGGAAGAGT TAAAAAAAGG TAGGGGCCGG GGCAGAGGAA GAGGAAGAGG AGCATGGAAA 
AAAGAACCTT CAAGGACACC GGCATTAAAA CGTGCGCAAT TGACAGATGA TGAAGATGAT 
GACAATGTGG ATGATGAAGA AAGTGATTGT GTAGAGCAAC GAAGAAAAAA ACCTAGGATA 
AACACCAGGA ACCAGGGCAG AAGAACTGTG TTATATAATG ATGAGTCTGA TAATGAGCAT 
GATGGTGCGG GGCAATATGA TGATCCCTTA AACCTGGGAA CATCCAGGTC TGGAAGAGTG 
AGAAAAATGA CGGAAAAGGC CAGAGTGAGT CATCTGATGG GATGGAATGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

brwd3 ( XM_018096621.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017952110.2 Xenopus tropicalis bromodomain and WD repeat domain containing 3 (brwd3), transcript variant X3, mRNA.

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CloneID	OXa64565
Clone ID Related Accession (Same CDS sequence)	XM_018096621.2
Accession Version	XM_018096621.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5319bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	bromodomain and WD repeat-containing protein 3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018096621.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCCG AGCTTTACTT CCTGATCGCC CGCTTCCTAC AGTCTGGCCC CTGCCGGAGA 
TCCGCACAGG TTCTGGCGGA GGAACTGGAG GAGTACCGGC TCATCCCACC GCGCTTAGAC 
TGGGAAGGGA AGGAACACCG CAGAACTTAT CAGGATTTGG TGGCAGCCAA TAAACACATC 
CCATCAGACT ACCTGCTTAA AATCTGCGAG CGTTTTGGAC CTATCCTAGA TCAAGAAATC 
CCGCAGAGTG TTCCTGGGTT GCATAGTCTG TTGGGACTTG GAAAACAGTC ACTGCTCAGA 
AGTAAAAAAG ACTGCAAGAA TACACCATGG AAAGGGACAG CTTTTGCAGC TTTGCACAGA 
GGGAGACCAC CAGAACTTCC AGTCAACTAT GGCAAGCCCC TCAACGTTGT AAATATCACT 
TGCGCCAGAT ATCAGACTGG ATATGCACAT TACCATCGCA TATTTCCATC TTCAACTTAT 
CAGCAGATGA AAATGCACAA GAGGATTTTG GGCCATCTTT CATCTGTATA CTGTGTGGCT 
TTTGATCGCA CTGGAAGACG TATTTTCACT GGTTCAGATG ACTGTCTAGT CAAAATATGG 
GCAAGTGATG ATGGACGTCT TCTTTCCACA CTACGTGGCC ATTCAGCTGA AATCTCTGAT 
CTGGCTGTGA ATCATGAGAA TACATTGATT GCTGCTGGCA GCTGTGATAA GGTGGTGCGT 
GTGTGGTGCC TTCGTACATG TGCACCAATA GCTGTGCTGC AGGGTCATAC AGCTTCTATC 
ACTTCCATAC AGTTTTGCCC AGGAACAAAA GAAAAAACAA GATACCTCAC ATCTACTGGT 
GCTGATGGGA CAATCTGCTT CTGGGAGTGG CATGCAGATA CCATGACATT TAATGATCGC 
CCTGTGAAAT TTGCAGAGAG GTCCAGGCCT GGGGTTCAGA TCTCCTGCTC ATCCTTTAGT 
TCAGGTGGTA TGTTTATAGC CACAGGAAGT ACTGATCATG TGATAAGAAT ATACTACCTG 
GGATCTGGTT CACCAGAGAA GATAGCTGAA TTGGAAGCAC ATTTGGACAA AGTGGTCGCT 
ATTCAGTTCT GCAACAATTC AGATCGATTG TGCTTTGTCA GTGGAAGCAG AGATGGCACT 
GCTCGGATTT GGCAATATCA GCAGCAAGAG TGGAAAATGA CTGTTCTGGA TATGACTGCC 
AAGATGGCTG GAAGCAGTGT TATAAATACA GAAGACAAAG TGACCAAGCT TAAAGTCACT 
ATGGTAGCAT GGGACCGATC TGATTCTTCG GTTATTACTG CTGTAAACAA TTTCTTTCTG 
AAAGTTTGGA ACTCTCATAC TGGCGAACTT CTTCATATAT TGTCTGGCCA TGATGATGAA 
GTTTTTGTTT TGGAACCTCA TCCTTTTGAT CCAAGGATTT TAATCTCTGC TGGGCATGAT 
GGAAACATAT TTGTGTGGGA CCTCGAAACA GGAACCAAAA TTCGTAATTA CTTTAACATG 
ATTGAAGGTC AGGGTCATGG AGCCGTGTTT GACTGTAAGT TCTCGCCTGA TGGTCAAAAC 
TTTGCCTGTA CAGATTCTCA TGGACATCTT TTACTCTTTG GTTTTGGTTG CAGTAAACCA 
TATGAAAAGA TTCCAGAACA GATGTTCTTC CATACAGACT TTAGACCTCT AATAAGAGAC 
TCCAACAGCT ATGTTTTGGA TGAACAAACT CAACAAGCCC CACACCTAAT GCCCCCTCCA 
TTCCTAGTAG ACGTTGATGG CAACCCACAT CCTACCCGTT TTCAGCGCTT GGTGCCAGGA 
CGTGAGAATT GTAAAGATGA GCAGCTCATA CCTCAGCTGG GATACATGTC AAATGGCAAT 
GGCGATGTGG TGGAGCAGGT CATTGGCCAG CAAACAGGAA ACCAGGAGGA AAGTATTCTT 
GATGGGATAA TCCGAGAACT CCAGAGGGAA CAGGATGAGA GGCATACAAA TGAAGCCGAG 
GATGTGGATA CACCCAGATA TAACAGAACA GACTCGAGAA ATGGAGTATT AAGAAGTATT 
AGCTTGGATT TATCATCTCC ACCTAATGTT GGCCTTCGCC GGAGTGGCCA AATTGAGGGT 
GTTCGCCAAA TGCATAGTAA TGCACCTCGG AGTCAAATTG CAACAGAAAG AGACCTAATG 
GCCTGGAGCA GAAGAGTGGT AGTAAATGAA CTGCATCCTG GTGTTATCAG GGTCCAGGAA 
GAATGCAGAA TTTCTAAAGG CGAAATGGAA ATCAGTTTGT ACAACACAGA GAAAAAGAGG 
AAACCAACTA ACCCTGCTCA GAGGAATGAT TATCAAGCTT TTGGTGGGCG ATCACTTAGA 
CGAATACAGC GGAAGCGCCA GCATGCCTAC CAGACACGTT CAACCATTGA ACATGATGCA 
AGAGCTAAAG TTCCAAGTAC AAATGGGGAA GATTCGCAGA GTTCCCCCGA GGAGGAGGAG 
TCCCAACATT CAACAGAGGC TTCAGGAGAT GAAGCTGCAG CTCAGTGGCA ATCAGAAACC 
TCTTCCAGTA ATTCATCTAG TGAATATTCC GACTGGACTG CAGATGCAGG AATCAATCTC 
CAGCCTCCCA AAAGACAGAC ACGGCAAACA ACAAGGAAAA TGTGCAGCAG TTCAGAGGAT 
GAGAACGCTA AAGAGGAAGA GAAGCCGAGG AAACACAAAC TGACCAGACG GAAGAAACAA 
AGTGAGCGTC TTTCTATGGA AGGAGAGCCA AGTGAAGAAT GGATTCCCCC CCAGTGGATC 
TTGGACACCA TCCCACGTCG TTCTCCTTTT GTTCCCCAGA TGGGAGATGA GGTTATTTAT 
TTCAGACAAG GCCATGAAGC TTATGTACGA GCAGTAAGGA AGGCTAAAAT ATATAATATT 
AATCCACAAA AGGAGCCATG GAACAAAACG GATCTCAGGG ATCAAGAATT TGCTAAGATT 
GTAGGAATTA AGTATGAGGT GGGGCCGCCT ACATTATGCT GCTTAAAACT TGCCTTACTG 
GATCCTACTT CAGGCAAAAT AACTGCAGAC TCTTTCTCTG TTAAATACCA TGACATGCCT 
GATGTTATTG ACTTCCTTGT CCTTTACCAG TTTTATAAGG AGGCGAAAGA GAGAAACTGG 
CAGATAGGAG ACCGGTTTCG AAGCATTATT GATGATGCAT GGTGGTTTGG CACAGTGGAA 
AGTCAGCAGC CATTCCAGAC TGAGTATCCT GACAGTTTAT TCCAGTGCTA CAGTGTACAC 
TGGGATAATA ATGAAAGAGA AAACATGAGT CCTTGGGACA TGGAACCAAT TCCAGATGGC 
ACAGCTTTGC CTGATGAAAT GGGTGCTGGT GTTCCCGTTA CTTCCGATGA ACTGAAGTCT 
TTGCAGTATA AACCGGAGGA GGGAGAGTGG GGATTGTACT CAAGAGATGA GGTTTGCGAA 
AGAGTTATCC AAGGAATTGA TCAGCTACTT ACTCTTGATT TTGCCAGCCC ATTCAGTGCT 
CCTGTGGATC TCAGTGCCTA CCCCTTATAC TGCACTGTGG TAGCATACCC AACAGATCTA 
AGCACCACAA GGAAGAGACT GGAGAGCCAC TACTATAGGA GGCTTTCTGC CCTAATATGG 
GAGGTCCGCT ATATTGAACA CAATGCAAGA ACATTCAATG AGCCTGACAC TCCAATTGTT 
AGAACTGCCA AAATTGTGAC AGATGTGCTG TTGCGTTATA TTGGGGATGA GAATTGTAAT 
GATATATTAG AGATCTACAA TCAAGTAAAG ACTGAAGATA CTGGCAATAC AGAAGATGAG 
GAGGGTGGAG CAGTTGATAT GGAATCAGAT GCCCCAGGGA CATCATCTGG TCGGGCTGTT 
ACCCGTCGCA CTAAAAGAGA TCTACTTCGT TCCAATCCTG ATTCATGGAA AGATAAATGC 
AAAGAACTAC TAGATTTAAT ATATGAGCGG GAAGATTCTG AACCCTTTAG GCAACCAGTC 
AATCAAGTCT CATATCCTGA TTATCAGGAT ATTATTGAAA CACCAATGGA TTTTAGCACC 
GTGAAAACAA CGTTAGAAGC TGGAAATTAT AGTGACCCGT TGGAATTTGC TGCAGATGTT 
CGACTAATCT TCAGTAACTC CAAAGCATAC ACTCCAAACA AAAAATCTAG AATCTACAGT 
ATGACTCTTC GACTCTCTGC ACTATGTGAA ACCTACATAA AGGATATCAT CTCTGACTAC 
AAACTAGCCA TGCAACTCCA ACAGAGAACA AGGCGGAAAT ACAGAAGGCG AATTAGGAGC 
AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC AGTAGTAATA GAAGAAGAAG TAGTAGTAGT 
AGTAGTAGTT CTAGTTCAGC ATCCAGCAGA GCATCCAGCC CAGACACAAA ACCTCGGAAA 
ATGAAGCTTC AACCGAAACT TAATCAGAAC CCTCCACATC CTCTTCCTAG GAGCAGCTCT 
TCATCCACTT CTCAAAAGCC TGATGGAAAG CCTTTTTCTG AAGATATGGA TCAAGAGCCA 
TCTTCATCTG GATGCAGCAA ACCTGAAACA CACAAAGAAC AGGAGAATGT GACCAAGAGC 
AAAAATTCTC CTGTGAAAGA GGAACATACA AATGGCCCTC TAACAAATGG CGATGGAGGA 
AACACACCAA AAACCAAGAG AAAGTTGCTA AGTTCTTCTG AGGATGAAAG TCCAGAAGAT 
GGAGAGCAAA TGGAAGAAAA GCCTGATAAA AGTCCAGCAT GTTCTCGTGA AAGAGACTCT 
GAATCAAGTT TAAGTTCGAA GAGTGAGTCA GAATCAGAGA GCAGTTCTGA TTCTGAATCC 
ATTAAGTCAG ATCAGGATTA TATTGATGGC GACCATCATT ATAGCAAAGA GCCCCGTACA 
AAGTTTAAGA GAAAATTTTG TAAATCTGGA GAGGCTAAAA CGAGAGGAAG GGGTCGAGGA 
AGAGGCAGAG GAAGAGGACG TGCAAAAGGT TATAATCGTG GTGTGGAGGA AGAGTTAAAA 
AAAGGTAGGG GCCGGGGCAG AGGAAGAGGA AGAGGAGCAT GGAAAAAAGA ACCTTCAAGG 
ACACCGGCAT TAAAACGTGC GCAATTGACA GATGATGAAG ATGATGACAA TGTGGATGAT 
GAAGAAAGTG ATTGTGTAGA GCAACGAAGA AAAAAACCTA GGATAAACAC CAGGAACCAG 
GGCAGAAGAA CTGTGTTATA TAATGATGAG TCTGATAATG AGCATGATGG TGCGGGGCAA 
TATGATGATC CCTTAAACCT GGGAACATCC AGGTCTGGAA GAGTGAGAAA AATGACGGAA 
AAGGCCAGAG TGAGTCATCT GATGGGATGG AATGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017952110.2
CDS	336..5654
Translation	MEAELYFLIARFLQSGPCRRSAQVLAEELEEYRLIPPRLDWEGKEHRRTYQDLVAANKHIPSDYLLKICERFGPILDQEIPQSVPGLHSLLGLGKQSLLRSKKDCKNTPWKGTAFAALHRGRPPELPVNYGKPLNVVNITCARYQTGYAHYHRIFPSSTYQQMKMHKRILGHLSSVYCVAFDRTGRRIFTGSDDCLVKIWASDDGRLLSTLRGHSAEISDLAVNHENTLIAAGSCDKVVRVWCLRTCAPIAVLQGHTASITSIQFCPGTKEKTRYLTSTGADGTICFWEWHADTMTFNDRPVKFAERSRPGVQISCSSFSSGGMFIATGSTDHVIRIYYLGSGSPEKIAELEAHLDKVVAIQFCNNSDRLCFVSGSRDGTARIWQYQQQEWKMTVLDMTAKMAGSSVINTEDKVTKLKVTMVAWDRSDSSVITAVNNFFLKVWNSHTGELLHILSGHDDEVFVLEPHPFDPRILISAGHDGNIFVWDLETGTKIRNYFNMIEGQGHGAVFDCKFSPDGQNFACTDSHGHLLLFGFGCSKPYEKIPEQMFFHTDFRPLIRDSNSYVLDEQTQQAPHLMPPPFLVDVDGNPHPTRFQRLVPGRENCKDEQLIPQLGYMSNGNGDVVEQVIGQQTGNQEESILDGIIRELQREQDERHTNEAEDVDTPRYNRTDSRNGVLRSISLDLSSPPNVGLRRSGQIEGVRQMHSNAPRSQIATERDLMAWSRRVVVNELHPGVIRVQEECRISKGEMEISLYNTEKKRKPTNPAQRNDYQAFGGRSLRRIQRKRQHAYQTRSTIEHDARAKVPSTNGEDSQSSPEEEESQHSTEASGDEAAAQWQSETSSSNSSSEYSDWTADAGINLQPPKRQTRQTTRKMCSSSEDENAKEEEKPRKHKLTRRKKQSERLSMEGEPSEEWIPPQWILDTIPRRSPFVPQMGDEVIYFRQGHEAYVRAVRKAKIYNINPQKEPWNKTDLRDQEFAKIVGIKYEVGPPTLCCLKLALLDPTSGKITADSFSVKYHDMPDVIDFLVLYQFYKEAKERNWQIGDRFRSIIDDAWWFGTVESQQPFQTEYPDSLFQCYSVHWDNNERENMSPWDMEPIPDGTALPDEMGAGVPVTSDELKSLQYKPEEGEWGLYSRDEVCERVIQGIDQLLTLDFASPFSAPVDLSAYPLYCTVVAYPTDLSTTRKRLESHYYRRLSALIWEVRYIEHNARTFNEPDTPIVRTAKIVTDVLLRYIGDENCNDILEIYNQVKTEDTGNTEDEEGGAVDMESDAPGTSSGRAVTRRTKRDLLRSNPDSWKDKCKELLDLIYEREDSEPFRQPVNQVSYPDYQDIIETPMDFSTVKTTLEAGNYSDPLEFAADVRLIFSNSKAYTPNKKSRIYSMTLRLSALCETYIKDIISDYKLAMQLQQRTRRKYRRRIRSSSSSSSSSSSSSNRRRSSSSSSSSSSASSRASSPDTKPRKMKLQPKLNQNPPHPLPRSSSSSTSQKPDGKPFSEDMDQEPSSSGCSKPETHKEQENVTKSKNSPVKEEHTNGPLTNGDGGNTPKTKRKLLSSSEDESPEDGEQMEEKPDKSPACSRERDSESSLSSKSESESESSSDSESIKSDQDYIDGDHHYSKEPRTKFKRKFCKSGEAKTRGRGRGRGRGRGRAKGYNRGVEEELKKGRGRGRGRGRGAWKKEPSRTPALKRAQLTDDEDDDNVDDEESDCVEQRRKKPRINTRNQGRRTVLYNDESDNEHDGAGQYDDPLNLGTSRSGRVRKMTEKARVSHLMGWNG

Target ORF information:

RefSeq Version	XM_018096621.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis bromodomain and WD repeat domain containing 3 (brwd3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096621.2

ORF Insert Sequence:

ATGGAAGCCG AGCTTTACTT CCTGATCGCC CGCTTCCTAC AGTCTGGCCC CTGCCGGAGA 
TCCGCACAGG TTCTGGCGGA GGAACTGGAG GAGTACCGGC TCATCCCACC GCGCTTAGAC 
TGGGAAGGGA AGGAACACCG CAGAACTTAT CAGGATTTGG TGGCAGCCAA TAAACACATC 
CCATCAGACT ACCTGCTTAA AATCTGCGAG CGTTTTGGAC CTATCCTAGA TCAAGAAATC 
CCGCAGAGTG TTCCTGGGTT GCATAGTCTG TTGGGACTTG GAAAACAGTC ACTGCTCAGA 
AGTAAAAAAG ACTGCAAGAA TACACCATGG AAAGGGACAG CTTTTGCAGC TTTGCACAGA 
GGGAGACCAC CAGAACTTCC AGTCAACTAT GGCAAGCCCC TCAACGTTGT AAATATCACT 
TGCGCCAGAT ATCAGACTGG ATATGCACAT TACCATCGCA TATTTCCATC TTCAACTTAT 
CAGCAGATGA AAATGCACAA GAGGATTTTG GGCCATCTTT CATCTGTATA CTGTGTGGCT 
TTTGATCGCA CTGGAAGACG TATTTTCACT GGTTCAGATG ACTGTCTAGT CAAAATATGG 
GCAAGTGATG ATGGACGTCT TCTTTCCACA CTACGTGGCC ATTCAGCTGA AATCTCTGAT 
CTGGCTGTGA ATCATGAGAA TACATTGATT GCTGCTGGCA GCTGTGATAA GGTGGTGCGT 
GTGTGGTGCC TTCGTACATG TGCACCAATA GCTGTGCTGC AGGGTCATAC AGCTTCTATC 
ACTTCCATAC AGTTTTGCCC AGGAACAAAA GAAAAAACAA GATACCTCAC ATCTACTGGT 
GCTGATGGGA CAATCTGCTT CTGGGAGTGG CATGCAGATA CCATGACATT TAATGATCGC 
CCTGTGAAAT TTGCAGAGAG GTCCAGGCCT GGGGTTCAGA TCTCCTGCTC ATCCTTTAGT 
TCAGGTGGTA TGTTTATAGC CACAGGAAGT ACTGATCATG TGATAAGAAT ATACTACCTG 
GGATCTGGTT CACCAGAGAA GATAGCTGAA TTGGAAGCAC ATTTGGACAA AGTGGTCGCT 
ATTCAGTTCT GCAACAATTC AGATCGATTG TGCTTTGTCA GTGGAAGCAG AGATGGCACT 
GCTCGGATTT GGCAATATCA GCAGCAAGAG TGGAAAATGA CTGTTCTGGA TATGACTGCC 
AAGATGGCTG GAAGCAGTGT TATAAATACA GAAGACAAAG TGACCAAGCT TAAAGTCACT 
ATGGTAGCAT GGGACCGATC TGATTCTTCG GTTATTACTG CTGTAAACAA TTTCTTTCTG 
AAAGTTTGGA ACTCTCATAC TGGCGAACTT CTTCATATAT TGTCTGGCCA TGATGATGAA 
GTTTTTGTTT TGGAACCTCA TCCTTTTGAT CCAAGGATTT TAATCTCTGC TGGGCATGAT 
GGAAACATAT TTGTGTGGGA CCTCGAAACA GGAACCAAAA TTCGTAATTA CTTTAACATG 
ATTGAAGGTC AGGGTCATGG AGCCGTGTTT GACTGTAAGT TCTCGCCTGA TGGTCAAAAC 
TTTGCCTGTA CAGATTCTCA TGGACATCTT TTACTCTTTG GTTTTGGTTG CAGTAAACCA 
TATGAAAAGA TTCCAGAACA GATGTTCTTC CATACAGACT TTAGACCTCT AATAAGAGAC 
TCCAACAGCT ATGTTTTGGA TGAACAAACT CAACAAGCCC CACACCTAAT GCCCCCTCCA 
TTCCTAGTAG ACGTTGATGG CAACCCACAT CCTACCCGTT TTCAGCGCTT GGTGCCAGGA 
CGTGAGAATT GTAAAGATGA GCAGCTCATA CCTCAGCTGG GATACATGTC AAATGGCAAT 
GGCGATGTGG TGGAGCAGGT CATTGGCCAG CAAACAGGAA ACCAGGAGGA AAGTATTCTT 
GATGGGATAA TCCGAGAACT CCAGAGGGAA CAGGATGAGA GGCATACAAA TGAAGCCGAG 
GATGTGGATA CACCCAGATA TAACAGAACA GACTCGAGAA ATGGAGTATT AAGAAGTATT 
AGCTTGGATT TATCATCTCC ACCTAATGTT GGCCTTCGCC GGAGTGGCCA AATTGAGGGT 
GTTCGCCAAA TGCATAGTAA TGCACCTCGG AGTCAAATTG CAACAGAAAG AGACCTAATG 
GCCTGGAGCA GAAGAGTGGT AGTAAATGAA CTGCATCCTG GTGTTATCAG GGTCCAGGAA 
GAATGCAGAA TTTCTAAAGG CGAAATGGAA ATCAGTTTGT ACAACACAGA GAAAAAGAGG 
AAACCAACTA ACCCTGCTCA GAGGAATGAT TATCAAGCTT TTGGTGGGCG ATCACTTAGA 
CGAATACAGC GGAAGCGCCA GCATGCCTAC CAGACACGTT CAACCATTGA ACATGATGCA 
AGAGCTAAAG TTCCAAGTAC AAATGGGGAA GATTCGCAGA GTTCCCCCGA GGAGGAGGAG 
TCCCAACATT CAACAGAGGC TTCAGGAGAT GAAGCTGCAG CTCAGTGGCA ATCAGAAACC 
TCTTCCAGTA ATTCATCTAG TGAATATTCC GACTGGACTG CAGATGCAGG AATCAATCTC 
CAGCCTCCCA AAAGACAGAC ACGGCAAACA ACAAGGAAAA TGTGCAGCAG TTCAGAGGAT 
GAGAACGCTA AAGAGGAAGA GAAGCCGAGG AAACACAAAC TGACCAGACG GAAGAAACAA 
AGTGAGCGTC TTTCTATGGA AGGAGAGCCA AGTGAAGAAT GGATTCCCCC CCAGTGGATC 
TTGGACACCA TCCCACGTCG TTCTCCTTTT GTTCCCCAGA TGGGAGATGA GGTTATTTAT 
TTCAGACAAG GCCATGAAGC TTATGTACGA GCAGTAAGGA AGGCTAAAAT ATATAATATT 
AATCCACAAA AGGAGCCATG GAACAAAACG GATCTCAGGG ATCAAGAATT TGCTAAGATT 
GTAGGAATTA AGTATGAGGT GGGGCCGCCT ACATTATGCT GCTTAAAACT TGCCTTACTG 
GATCCTACTT CAGGCAAAAT AACTGCAGAC TCTTTCTCTG TTAAATACCA TGACATGCCT 
GATGTTATTG ACTTCCTTGT CCTTTACCAG TTTTATAAGG AGGCGAAAGA GAGAAACTGG 
CAGATAGGAG ACCGGTTTCG AAGCATTATT GATGATGCAT GGTGGTTTGG CACAGTGGAA 
AGTCAGCAGC CATTCCAGAC TGAGTATCCT GACAGTTTAT TCCAGTGCTA CAGTGTACAC 
TGGGATAATA ATGAAAGAGA AAACATGAGT CCTTGGGACA TGGAACCAAT TCCAGATGGC 
ACAGCTTTGC CTGATGAAAT GGGTGCTGGT GTTCCCGTTA CTTCCGATGA ACTGAAGTCT 
TTGCAGTATA AACCGGAGGA GGGAGAGTGG GGATTGTACT CAAGAGATGA GGTTTGCGAA 
AGAGTTATCC AAGGAATTGA TCAGCTACTT ACTCTTGATT TTGCCAGCCC ATTCAGTGCT 
CCTGTGGATC TCAGTGCCTA CCCCTTATAC TGCACTGTGG TAGCATACCC AACAGATCTA 
AGCACCACAA GGAAGAGACT GGAGAGCCAC TACTATAGGA GGCTTTCTGC CCTAATATGG 
GAGGTCCGCT ATATTGAACA CAATGCAAGA ACATTCAATG AGCCTGACAC TCCAATTGTT 
AGAACTGCCA AAATTGTGAC AGATGTGCTG TTGCGTTATA TTGGGGATGA GAATTGTAAT 
GATATATTAG AGATCTACAA TCAAGTAAAG ACTGAAGATA CTGGCAATAC AGAAGATGAG 
GAGGGTGGAG CAGTTGATAT GGAATCAGAT GCCCCAGGGA CATCATCTGG TCGGGCTGTT 
ACCCGTCGCA CTAAAAGAGA TCTACTTCGT TCCAATCCTG ATTCATGGAA AGATAAATGC 
AAAGAACTAC TAGATTTAAT ATATGAGCGG GAAGATTCTG AACCCTTTAG GCAACCAGTC 
AATCAAGTCT CATATCCTGA TTATCAGGAT ATTATTGAAA CACCAATGGA TTTTAGCACC 
GTGAAAACAA CGTTAGAAGC TGGAAATTAT AGTGACCCGT TGGAATTTGC TGCAGATGTT 
CGACTAATCT TCAGTAACTC CAAAGCATAC ACTCCAAACA AAAAATCTAG AATCTACAGT 
ATGACTCTTC GACTCTCTGC ACTATGTGAA ACCTACATAA AGGATATCAT CTCTGACTAC 
AAACTAGCCA TGCAACTCCA ACAGAGAACA AGGCGGAAAT ACAGAAGGCG AATTAGGAGC 
AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC AGTAGTAATA GAAGAAGAAG TAGTAGTAGT 
AGTAGTAGTT CTAGTTCAGC ATCCAGCAGA GCATCCAGCC CAGACACAAA ACCTCGGAAA 
ATGAAGCTTC AACCGAAACT TAATCAGAAC CCTCCACATC CTCTTCCTAG GAGCAGCTCT 
TCATCCACTT CTCAAAAGCC TGATGGAAAG CCTTTTTCTG AAGATATGGA TCAAGAGCCA 
TCTTCATCTG GATGCAGCAA ACCTGAAACA CACAAAGAAC AGGAGAATGT GACCAAGAGC 
AAAAATTCTC CTGTGAAAGA GGAACATACA AATGGCCCTC TAACAAATGG CGATGGAGGA 
AACACACCAA AAACCAAGAG AAAGTTGCTA AGTTCTTCTG AGGATGAAAG TCCAGAAGAT 
GGAGAGCAAA TGGAAGAAAA GCCTGATAAA AGTCCAGCAT GTTCTCGTGA AAGAGACTCT 
GAATCAAGTT TAAGTTCGAA GAGTGAGTCA GAATCAGAGA GCAGTTCTGA TTCTGAATCC 
ATTAAGTCAG ATCAGGATTA TATTGATGGC GACCATCATT ATAGCAAAGA GCCCCGTACA 
AAGTTTAAGA GAAAATTTTG TAAATCTGGA GAGGCTAAAA CGAGAGGAAG GGGTCGAGGA 
AGAGGCAGAG GAAGAGGACG TGCAAAAGGT TATAATCGTG GTGTGGAGGA AGAGTTAAAA 
AAAGGTAGGG GCCGGGGCAG AGGAAGAGGA AGAGGAGCAT GGAAAAAAGA ACCTTCAAGG 
ACACCGGCAT TAAAACGTGC GCAATTGACA GATGATGAAG ATGATGACAA TGTGGATGAT 
GAAGAAAGTG ATTGTGTAGA GCAACGAAGA AAAAAACCTA GGATAAACAC CAGGAACCAG 
GGCAGAAGAA CTGTGTTATA TAATGATGAG TCTGATAATG AGCATGATGG TGCGGGGCAA 
TATGATGATC CCTTAAACCT GGGAACATCC AGGTCTGGAA GAGTGAGAAA AATGACGGAA 
AAGGCCAGAG TGAGTCATCT GATGGGATGG AATGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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brwd3 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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