Brwd3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Brwd3
Entrez Gene ID	382236
Full Name	bromodomain and WD repeat domain containing 3
Synonyms	Brodl,D030064D06Rik,Gm596
General protein information	Preferred Names bromodomain and WD repeat domain containing 3 Names bromodomain and WD repeat-containing protein 3 bromo domain-containing protein disrupted in leukemia
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: X Map Location: X\|X D

mRNA and Protein(s)

mRNA	Protein	Name
NM_001081477.1	NP_001074946.1	bromodomain and WD repeat-containing protein 3
XM_006528114.3	XP_006528177.1	bromodomain and WD repeat-containing protein 3 isoform X1
NM_001081477.2	NP_001074946.1	bromodomain and WD repeat-containing protein 3

Pathways from BioSystems

Homologies

Homo sapiens (human)	BRWD3	NP_694984.4
Macaca mulatta (Rhesus monkey)	BRWD3	XP_002806340.1
Canis lupus familiaris (dog)	BRWD3	XP_005641586.1
Mus musculus (house mouse)	Brwd3	NP_001074946.1
Rattus norvegicus (Norway rat)	Brwd3	XP_006257245.1
Gallus gallus (chicken)	BRWD3	XP_003641127.2
Bos taurus (cattle)	BRWD3	XP_002699983.1
Xenopus tropicalis (tropical clawed frog)	brwd3	XP_002940482.2
Pan troglodytes (chimpanzee)	BRWD3	XP_003954075.1

Gene Ontology
Bibliography

Related articles in PubMed

Epigenetic functions of smchd1 repress gene clusters on the inactive X chromosome and on autosomes.
Gendrel AV, Tang YA, Suzuki M, Godwin J, Nesterova TB, Greally JM, Heard E, Brockdorff N
Molecular and cellular biology33(16)3150-65(2013 Aug)

Phenotypic annotation of the mouse X chromosome.
Cox BJ, Vollmer M, Tamplin O, Lu M, Biechele S, Gertsenstein M, van Campenhout C, Floss T, Kühn R, Wurst W, Lickert H, Rossant J
Genome research20(8)1154-64(2010 Aug)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Brwd3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Brwd3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu02510	NM_001081477.2 Latest version!	Mus musculus bromodomain and WD repeat domain containing 3 (Brwd3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu56305	XM_006528114.3 Latest version!	Mus musculus bromodomain and WD repeat domain containing 3 (Brwd3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OMu02510	NM_001081477.1	Mus musculus bromodomain and WD repeat domain containing 3 (Brwd3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Brwd3 ( NM_001081477.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001074946.1 Mus musculus bromodomain and WD repeat domain containing 3 (Brwd3), mRNA.

Price & Availability↑

CloneID	OMu02510
Clone ID Related Accession (Same CDS sequence)	NM_001081477.1 , NM_001081477.2
Accession Version	NM_001081477.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5400bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1509206400000
Organism	Mus musculus(house mouse)
Product	bromodomain and WD repeat-containing protein 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL731764.11 and BX000698.11. On Feb 9, 2007 this sequence version replaced XM_897923.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGCGG CACCTACCCA GATCGAAGCC GAGCTGTATT ACCTGATCGC AAGGTTCTTG 
CAGTCCGGAC CCTGTAACAA ATCCGCTCAG GTGCTAGTGC AAGAGCTCGA AGAGCATCAG 
CTGATTCCGC GCCGCTTAGA CTGGGAAGGG AAAGAGCACC GAAGAAGCTT CGAGGATCTG 
GTGGCAGCCA ATGCACACAT TCCTCCAGAC TACCTCCTTA AAATTTGTGA GAGGATTGGC 
CCTTTATTAG ATAAGGAAAT CCCTCAGAGT GTTCCTGGGG TACAGACATT ACTAGGTGTC 
GGTCGGCAGT CTCTGTTACG GGATGCCAAA GACTGTAAGA GTACACTATG GAATGGGTCT 
GCTTTTGCAG CCCTCCACAG AGGCAGACCC CCAGAACTAC CTGTAAATTA TGTGAAACCT 
CCAAACGTGG TGAATATCAC CTCAGCCAGG CAATTAACCG GATGTAGTCG CTTCAGCCAT 
GTTTTCCCCT CATCTGCTTA CCAGCACATT AAGATGCATA AGAGAATTCT GGGACACTTA 
TCTTCTGTCT ACTGTGTAGC CTTTGATCGA AGCGGGAGGC GAATTTTTAC AGGTTCAGAT 
GACTGTTTGG TGAAAATCTG GGCAACAGAT GATGGACGCC TCCTTGCCAC ACTGCGAGGG 
CACTCTGCTG AAATCTCCGA CATGGCTGTT AATTATGAAA ATACTCTCAT TGCTGCAGGC 
AGCTGTGATA AAGTTGTGAG AGTATGGTGT CTTCGTACTT GTGCACCAGT TGCTGTCCTT 
CAGGGACATT CAGCTTCTAT TACTTCCATA CAGTTTTGTC CATCAACTAA AGGCACAACT 
AGATACCTCA CTTCTACTGG TGCTGATGGA ACAATTTGTT TCTGGCAATG GCATGTAAAA 
ACAATGAAGT TTAGAGATCG CCCAGTAAAA TTTACTGAGA GATCCAGACC TGGAGTTCAG 
ATATCTTGTT CATCTTTCAG TTCTGGTGGT ATGTTCATTA CAACAGGGAG CACTGATCAT 
GTAATTAGGA TATACTATTT GGGTTCTGAG ATACCTGAGA AAATTGCTGA ATTAGAATCA 
CATACGGACA AAGTTGTTGC TGTTCAGTTC TGTAACAACG GAGACAGCTT AAGGTTTGTT 
AGTGGAAGTA GAGACGGAAC AGCAAGAATA TGGCAATATC AACAACAAGA GTGGAAGAGC 
ATAGTGCTAG ATATGGCTAC TAAAATGAGT GGGAATAATT TGACATCAGC TGAAGACAAA 
GTCACTAAAC TAAAGGTGAC TATGGTGGCT TGGGATCGGT ATGATACCAC TGTTATTACT 
GCAGTGAACA ATTTCCTTTT GAAAGTTTGG AATTCTGTCA CAGGGCAGCT TCTTCATACT 
TTATCTGGGC ATGATGATGA AGTATTTGTT CTAGAAGCAC ATCCATTTGA TCAAAGGATC 
ATACTTTCAG CAGGTCATGA TGGGAACATT TTTATTTGGG ACCTTGACCG GGGGACCAAA 
ATACGAAATT ACTTTAATAT GATCGAAGGC CAAGGTCATG GTGCAGTATT TGATTGTAAA 
TTTTCACCAG ATGGAAACCA TTTTGCCTGT ACAGACTCTC ATGGGCATTT GTTGCTATTT 
GGCTTTGGAT GCAGTAAATA CTATGAAAAG ATTCCAGATC AGATGTTCTT CCATACCGAT 
TATCGGCCCC TCATCAGAGA TGCCAATAAC TATGTATTAG ATGAACAAAC TCAACAAGCT 
CCTCACCTCA TGCCTCCCCC ATTCTTGGTG GATGTCGATG GAAATCCTCA TCCTACAAAA 
TTTCAACGGC TGGTACCAGG ACGGGAAAAC TGTAAAGATG AACAACTTAT TCCACAGTTA 
GGATATGTGG CTAATGGTGA CGGTGAGGTG GTAGAACAGG TAATTGGGCA GCAAACCAAT 
GACCAAGAAG AAAGCATTCT TGATGGAATA ATCAGGGAGT TACAGAGAGA ACAAGATCTC 
AGATTAATTA ATGAAGGAGA TGTTCCACAT TTTCCAATTA ATAGATCATA TTCTGTTAAT 
GGTGCTCTGA GTAGTCCAAA TATGGACATA CCTTCTTCCC CAAATATTGG CCTTCGGCGT 
AGTGGCCAAA TTGAAGGTGT TCGGCAGATG CACAACAATG CTCCCCGGAG CCAGATGGCC 
ACTGAAAGAG ATCTCATGGC CTGGAGCCGA AGAGTGGTGG TGAATGAGCT AAATAGTGGC 
GTTAGTAGGG TACAAGAAGA ATGTCGAAAT GCAAAAGGTG ACCTAGAAGT TAGTCTTTAT 
ACTGTTGAAA AGAAGAAAAA GCCATCTTAT CCTATCCAAA GAAATGATTA TCAGCCCAGC 
TGTGGAAGGT CTTTACGAAG GACACAACGC AAGCGACAGC ATACTTACCT AACAAGGTCG 
AACATAGAGC ATAATTCACA GGCATCATCT CAAACTTCCG GTGTACAAGA AGATTCAGAC 
AGCTCTTCAG AGGAAGATGA AACTGTTGGC ACAAGTGATG CTTCCGTAGA AGACCCTGTT 
GTTGAGTGGC AAAGTGAGAG TTCTTCCAGC GATTCATCAA GTGAATATTC TGACTGGATA 
GCAGATGCTG GGATAAATTT GCAGCCTCCC AAAAGACAAA CAAGACAGGC AACACAGAAA 
ATATACAGCA GTTCTGAAGA TGAAAATTTG AAATTAGAAG ATAGGCAGAA GAAACCTAAA 
CAGACTAAAA AGAAGAAAGG GGGACTTGTT TCTATGGCAG GCGAACCCAA TGAAGAATGG 
CTTGCCCCTC AGTGGATCTT GGATACCATA CCACGACGTT CTCCTTTTGT TCCACAGATG 
GGAGATGAGC TTATTTACTT TCGACAAGGA CATGAAGCTT ATGTGCGAGC TGTAAGGAAA 
TCAAAAATCT ATAGTGTTAA TTTACAAAAA CAGCCATGGA ACAAAATGGA TCTCAGGGAG 
CAAGAATTTG TGAAGATTGT AGGAATCAAA TATGAGATTG GACCACCTAC GCTATGCTGC 
TTGAAACTTG CATTTTTGGA TCCAATTTCA GGCAAAATGA CTGGAGAGTC TTTTTCCATT 
AAGTATCATG ACATGCCAGA TGTCATTGAT TTCCTTGTGT TACATCAGTT TTATAATGAA 
GCCAAAGAAA GAAACTGGCA GATTGGTGAT AGATTCCGCA GTATAATAGA TGATGCCTGG 
TGGTTTGGGA CTGTGGAAAG TCAGCAGCCT TTTCAGCCAG AATATCCTGA TAGCTCCTTC 
CAGTGTTACA GTGTTCACTG GGACAATAAT GAAAGAGAAA AAATGAGCCC CTGGGATATG 
GAGCCGATTC CAGATGGAAC TGCTTTTCCA GATGAAGTTG GTGCTGGTAT TCCTGTGTCC 
CAGGAAGAAC TAACTGCATT GCTCTACAAA CCCCAGGAAG GAGAGTGGGG TGCTCATTCC 
AGAGATGAGG AGTGTGAACG AGTTATTCAG GGCATCAACA ACCTTCTTTC CCTGGATTTT 
GCCAGCCCTT TTGCTGTTCC AGTGGATCTC AGTGCCTACC CCTTGTATTG TACTGTAGTT 
GCTTATCCAA CTGACCTCAA TACCATCAAA CAAAGACTTG AAAATCGCTT TTACAGGAGA 
ATATCAGCAT TAATGTGGGA GGTACGCTAT ATTGAACACA ATGCTAGGAC TTTCAATGAG 
CCAGATAGTC CCATAGTTAA AGCAGCCAAG ATTGTGACTG ATGTCCTACT TCGGTTTATT 
GGGGATCAAA GCTGTTCTGA TATTTTGGAT ACTTACAATA AAATCAAAGC AGAAGATCCA 
GACAGCAGCG ATCTGGAGGA GGATTCAGAA ATGGTTGACT TAGATTCAGA TGGTCCTGGC 
ACTTCATCTG GAAGGAGGGC TAAATGTCGA GGCAGACGAC AGTCTCTCAA GTGTAATCCT 
GATGCTTGGA AAAAGCAATG CGAAGAGCTA CTGAGCCTCA TTTATGAACG GGAAGACTCA 
GAGCCATTTC GTCAGCCAGC AGATCCTCAA TCTTACCCAG TTCAACAGCA ACAAGAAGGG 
GAATCCTCAC AATCAGTTCC TCCAGATAGG CAAGACCCAT CTCTTTCTGA GGATTATCAA 
GATGGTATAG ACACACCTAT GGACTTCAGC ACTGTGAAAG AAACTTTGGA ATCAGGAAAT 
TATGATAGTC CTCTGGAATT TTATAAAGAT GTTCGCCAAA TATTCAGCAA CTCCAAAGCT 
TATACTTCTA ATAAAAAGTC AAGGATCTAT AGCATGACGC TGAGATTGTC AGCCTTATTT 
GAAAATCACA TTAAAAACAT CATCTCTGAC TATAAGTCAG CAATCCAGAG TCAGAAGAGG 
AGAAGACCAC GGTACAGAAA GCGTCTGAGA AGCAGCAGCA GCTCGTTATC TAGCAGCAGA 
GCTCCCAGTC CAAAAGGCAA ACAGAAGCAA ATGAAGTTAC AGCCTAAAAA TGACCAGAAT 
ACCTCAGTGG CTTATGCCAG GACTAGCTCT CCTTTTTCTT CTCCTGTTTC AGATGCTGCT 
GAAGGTGTTT CACTGTATCT ACTTGATGAT GAAGGGGATG GGCCTTTTTC TCCATCAAGT 
TTCAGTGGAT ATAGTAGAAG TGGAAATAGC CATGACCCAG GGAAAGCCAA ATCATTTCGT 
AATAGAGTTT TACCAGCTAA ACAAGATCAC TCCCTTGATG GACCTCTTAC AAATGTTGAT 
GGCAGAGAGC CCCGGACAGG AGCCAAAAGA AAACTGCTCA GTGCTTCAGA AGAAGATGAA 
AGCATGGGAG GAGAAGAGAA AGAAATGAAA GAAACAAAGG AGCAAGTACA TTTATCCTCC 
TCTGAGAGTG GAGAGTTAGG CTCCAGTTTA AGTTCAGAAA GTACCTCTGG CTCTGACTCT 
GACTCTGAAT CCACTTCCAG AACCGATCAA GATTATGTTG ATGGAGACCA TGACTATAGC 
AAATTCATAC AAACCAGACC TAAAAGGAAA CTCAGAAAAC AACACACCAA TGGAAAAAGA 
AACTGGAAGA CGAGAGGCAC AGGAGGAAGA GGCAGATGGG GAAGATGGGG AAGATGGAGC 
AGAGGAGGCA GAGGAAGAGG TGGCAGGGGT CGAGGGGGTC GAGGGCGAGG TGGAGGTGGG 
GGGAGAGGGA GAGGACGAGG GAGAGGAGGA AGAGGTGCCT CTAGAGGATC AAGCCGAGCC 
AAACGAGCCC GAGTTGCAGA TGATGAATTT GATACGATGT TTTCAGGACG TTTCAGTCGA 
CTTCCACGGA TTAAAACAAG AAACCAAGGC AGGAGAACTG TTTTATATAA TGATGATTCA 
GATAATGACA ACTTTGTGTC CACTGAGGAT CCTCTGAATC TTGGTACATC CAGATCAGGC 
AGAGTACGTA AAATGACCGA AAAAGCCAGG GTTAGCCATC TCATGGGATG GAATTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074946.1
CDS	227..5626
Translation	MAAAPTQIEAELYYLIARFLQSGPCNKSAQVLVQELEEHQLIPRRLDWEGKEHRRSFEDLVAANAHIPPDYLLKICERIGPLLDKEIPQSVPGVQTLLGVGRQSLLRDAKDCKSTLWNGSAFAALHRGRPPELPVNYVKPPNVVNITSARQLTGCSRFSHVFPSSAYQHIKMHKRILGHLSSVYCVAFDRSGRRIFTGSDDCLVKIWATDDGRLLATLRGHSAEISDMAVNYENTLIAAGSCDKVVRVWCLRTCAPVAVLQGHSASITSIQFCPSTKGTTRYLTSTGADGTICFWQWHVKTMKFRDRPVKFTERSRPGVQISCSSFSSGGMFITTGSTDHVIRIYYLGSEIPEKIAELESHTDKVVAVQFCNNGDSLRFVSGSRDGTARIWQYQQQEWKSIVLDMATKMSGNNLTSAEDKVTKLKVTMVAWDRYDTTVITAVNNFLLKVWNSVTGQLLHTLSGHDDEVFVLEAHPFDQRIILSAGHDGNIFIWDLDRGTKIRNYFNMIEGQGHGAVFDCKFSPDGNHFACTDSHGHLLLFGFGCSKYYEKIPDQMFFHTDYRPLIRDANNYVLDEQTQQAPHLMPPPFLVDVDGNPHPTKFQRLVPGRENCKDEQLIPQLGYVANGDGEVVEQVIGQQTNDQEESILDGIIRELQREQDLRLINEGDVPHFPINRSYSVNGALSSPNMDIPSSPNIGLRRSGQIEGVRQMHNNAPRSQMATERDLMAWSRRVVVNELNSGVSRVQEECRNAKGDLEVSLYTVEKKKKPSYPIQRNDYQPSCGRSLRRTQRKRQHTYLTRSNIEHNSQASSQTSGVQEDSDSSSEEDETVGTSDASVEDPVVEWQSESSSSDSSSEYSDWIADAGINLQPPKRQTRQATQKIYSSSEDENLKLEDRQKKPKQTKKKKGGLVSMAGEPNEEWLAPQWILDTIPRRSPFVPQMGDELIYFRQGHEAYVRAVRKSKIYSVNLQKQPWNKMDLREQEFVKIVGIKYEIGPPTLCCLKLAFLDPISGKMTGESFSIKYHDMPDVIDFLVLHQFYNEAKERNWQIGDRFRSIIDDAWWFGTVESQQPFQPEYPDSSFQCYSVHWDNNEREKMSPWDMEPIPDGTAFPDEVGAGIPVSQEELTALLYKPQEGEWGAHSRDEECERVIQGINNLLSLDFASPFAVPVDLSAYPLYCTVVAYPTDLNTIKQRLENRFYRRISALMWEVRYIEHNARTFNEPDSPIVKAAKIVTDVLLRFIGDQSCSDILDTYNKIKAEDPDSSDLEEDSEMVDLDSDGPGTSSGRRAKCRGRRQSLKCNPDAWKKQCEELLSLIYEREDSEPFRQPADPQSYPVQQQQEGESSQSVPPDRQDPSLSEDYQDGIDTPMDFSTVKETLESGNYDSPLEFYKDVRQIFSNSKAYTSNKKSRIYSMTLRLSALFENHIKNIISDYKSAIQSQKRRRPRYRKRLRSSSSSLSSSRAPSPKGKQKQMKLQPKNDQNTSVAYARTSSPFSSPVSDAAEGVSLYLLDDEGDGPFSPSSFSGYSRSGNSHDPGKAKSFRNRVLPAKQDHSLDGPLTNVDGREPRTGAKRKLLSASEEDESMGGEEKEMKETKEQVHLSSSESGELGSSLSSESTSGSDSDSESTSRTDQDYVDGDHDYSKFIQTRPKRKLRKQHTNGKRNWKTRGTGGRGRWGRWGRWSRGGRGRGGRGRGGRGRGGGGGRGRGRGRGGRGASRGSSRAKRARVADDEFDTMFSGRFSRLPRIKTRNQGRRTVLYNDDSDNDNFVSTEDPLNLGTSRSGRVRKMTEKARVSHLMGWNY

Target ORF information:

RefSeq Version	NM_001081477.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus bromodomain and WD repeat domain containing 3 (Brwd3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081477.1

ORF Insert Sequence:

ATGGCGGCGG CACCTACCCA GATCGAAGCC GAGCTGTATT ACCTGATCGC AAGGTTCTTG 
CAGTCCGGAC CCTGTAACAA ATCCGCTCAG GTGCTAGTGC AAGAGCTCGA AGAGCATCAG 
CTGATTCCGC GCCGCTTAGA CTGGGAAGGG AAAGAGCACC GAAGAAGCTT CGAGGATCTG 
GTGGCAGCCA ATGCACACAT TCCTCCAGAC TACCTCCTTA AAATTTGTGA GAGGATTGGC 
CCTTTATTAG ATAAGGAAAT CCCTCAGAGT GTTCCTGGGG TACAGACATT ACTAGGTGTC 
GGTCGGCAGT CTCTGTTACG GGATGCCAAA GACTGTAAGA GTACACTATG GAATGGGTCT 
GCTTTTGCAG CCCTCCACAG AGGCAGACCC CCAGAACTAC CTGTAAATTA TGTGAAACCT 
CCAAACGTGG TGAATATCAC CTCAGCCAGG CAATTAACCG GATGTAGTCG CTTCAGCCAT 
GTTTTCCCCT CATCTGCTTA CCAGCACATT AAGATGCATA AGAGAATTCT GGGACACTTA 
TCTTCTGTCT ACTGTGTAGC CTTTGATCGA AGCGGGAGGC GAATTTTTAC AGGTTCAGAT 
GACTGTTTGG TGAAAATCTG GGCAACAGAT GATGGACGCC TCCTTGCCAC ACTGCGAGGG 
CACTCTGCTG AAATCTCCGA CATGGCTGTT AATTATGAAA ATACTCTCAT TGCTGCAGGC 
AGCTGTGATA AAGTTGTGAG AGTATGGTGT CTTCGTACTT GTGCACCAGT TGCTGTCCTT 
CAGGGACATT CAGCTTCTAT TACTTCCATA CAGTTTTGTC CATCAACTAA AGGCACAACT 
AGATACCTCA CTTCTACTGG TGCTGATGGA ACAATTTGTT TCTGGCAATG GCATGTAAAA 
ACAATGAAGT TTAGAGATCG CCCAGTAAAA TTTACTGAGA GATCCAGACC TGGAGTTCAG 
ATATCTTGTT CATCTTTCAG TTCTGGTGGT ATGTTCATTA CAACAGGGAG CACTGATCAT 
GTAATTAGGA TATACTATTT GGGTTCTGAG ATACCTGAGA AAATTGCTGA ATTAGAATCA 
CATACGGACA AAGTTGTTGC TGTTCAGTTC TGTAACAACG GAGACAGCTT AAGGTTTGTT 
AGTGGAAGTA GAGACGGAAC AGCAAGAATA TGGCAATATC AACAACAAGA GTGGAAGAGC 
ATAGTGCTAG ATATGGCTAC TAAAATGAGT GGGAATAATT TGACATCAGC TGAAGACAAA 
GTCACTAAAC TAAAGGTGAC TATGGTGGCT TGGGATCGGT ATGATACCAC TGTTATTACT 
GCAGTGAACA ATTTCCTTTT GAAAGTTTGG AATTCTGTCA CAGGGCAGCT TCTTCATACT 
TTATCTGGGC ATGATGATGA AGTATTTGTT CTAGAAGCAC ATCCATTTGA TCAAAGGATC 
ATACTTTCAG CAGGTCATGA TGGGAACATT TTTATTTGGG ACCTTGACCG GGGGACCAAA 
ATACGAAATT ACTTTAATAT GATCGAAGGC CAAGGTCATG GTGCAGTATT TGATTGTAAA 
TTTTCACCAG ATGGAAACCA TTTTGCCTGT ACAGACTCTC ATGGGCATTT GTTGCTATTT 
GGCTTTGGAT GCAGTAAATA CTATGAAAAG ATTCCAGATC AGATGTTCTT CCATACCGAT 
TATCGGCCCC TCATCAGAGA TGCCAATAAC TATGTATTAG ATGAACAAAC TCAACAAGCT 
CCTCACCTCA TGCCTCCCCC ATTCTTGGTG GATGTCGATG GAAATCCTCA TCCTACAAAA 
TTTCAACGGC TGGTACCAGG ACGGGAAAAC TGTAAAGATG AACAACTTAT TCCACAGTTA 
GGATATGTGG CTAATGGTGA CGGTGAGGTG GTAGAACAGG TAATTGGGCA GCAAACCAAT 
GACCAAGAAG AAAGCATTCT TGATGGAATA ATCAGGGAGT TACAGAGAGA ACAAGATCTC 
AGATTAATTA ATGAAGGAGA TGTTCCACAT TTTCCAATTA ATAGATCATA TTCTGTTAAT 
GGTGCTCTGA GTAGTCCAAA TATGGACATA CCTTCTTCCC CAAATATTGG CCTTCGGCGT 
AGTGGCCAAA TTGAAGGTGT TCGGCAGATG CACAACAATG CTCCCCGGAG CCAGATGGCC 
ACTGAAAGAG ATCTCATGGC CTGGAGCCGA AGAGTGGTGG TGAATGAGCT AAATAGTGGC 
GTTAGTAGGG TACAAGAAGA ATGTCGAAAT GCAAAAGGTG ACCTAGAAGT TAGTCTTTAT 
ACTGTTGAAA AGAAGAAAAA GCCATCTTAT CCTATCCAAA GAAATGATTA TCAGCCCAGC 
TGTGGAAGGT CTTTACGAAG GACACAACGC AAGCGACAGC ATACTTACCT AACAAGGTCG 
AACATAGAGC ATAATTCACA GGCATCATCT CAAACTTCCG GTGTACAAGA AGATTCAGAC 
AGCTCTTCAG AGGAAGATGA AACTGTTGGC ACAAGTGATG CTTCCGTAGA AGACCCTGTT 
GTTGAGTGGC AAAGTGAGAG TTCTTCCAGC GATTCATCAA GTGAATATTC TGACTGGATA 
GCAGATGCTG GGATAAATTT GCAGCCTCCC AAAAGACAAA CAAGACAGGC AACACAGAAA 
ATATACAGCA GTTCTGAAGA TGAAAATTTG AAATTAGAAG ATAGGCAGAA GAAACCTAAA 
CAGACTAAAA AGAAGAAAGG GGGACTTGTT TCTATGGCAG GCGAACCCAA TGAAGAATGG 
CTTGCCCCTC AGTGGATCTT GGATACCATA CCACGACGTT CTCCTTTTGT TCCACAGATG 
GGAGATGAGC TTATTTACTT TCGACAAGGA CATGAAGCTT ATGTGCGAGC TGTAAGGAAA 
TCAAAAATCT ATAGTGTTAA TTTACAAAAA CAGCCATGGA ACAAAATGGA TCTCAGGGAG 
CAAGAATTTG TGAAGATTGT AGGAATCAAA TATGAGATTG GACCACCTAC GCTATGCTGC 
TTGAAACTTG CATTTTTGGA TCCAATTTCA GGCAAAATGA CTGGAGAGTC TTTTTCCATT 
AAGTATCATG ACATGCCAGA TGTCATTGAT TTCCTTGTGT TACATCAGTT TTATAATGAA 
GCCAAAGAAA GAAACTGGCA GATTGGTGAT AGATTCCGCA GTATAATAGA TGATGCCTGG 
TGGTTTGGGA CTGTGGAAAG TCAGCAGCCT TTTCAGCCAG AATATCCTGA TAGCTCCTTC 
CAGTGTTACA GTGTTCACTG GGACAATAAT GAAAGAGAAA AAATGAGCCC CTGGGATATG 
GAGCCGATTC CAGATGGAAC TGCTTTTCCA GATGAAGTTG GTGCTGGTAT TCCTGTGTCC 
CAGGAAGAAC TAACTGCATT GCTCTACAAA CCCCAGGAAG GAGAGTGGGG TGCTCATTCC 
AGAGATGAGG AGTGTGAACG AGTTATTCAG GGCATCAACA ACCTTCTTTC CCTGGATTTT 
GCCAGCCCTT TTGCTGTTCC AGTGGATCTC AGTGCCTACC CCTTGTATTG TACTGTAGTT 
GCTTATCCAA CTGACCTCAA TACCATCAAA CAAAGACTTG AAAATCGCTT TTACAGGAGA 
ATATCAGCAT TAATGTGGGA GGTACGCTAT ATTGAACACA ATGCTAGGAC TTTCAATGAG 
CCAGATAGTC CCATAGTTAA AGCAGCCAAG ATTGTGACTG ATGTCCTACT TCGGTTTATT 
GGGGATCAAA GCTGTTCTGA TATTTTGGAT ACTTACAATA AAATCAAAGC AGAAGATCCA 
GACAGCAGCG ATCTGGAGGA GGATTCAGAA ATGGTTGACT TAGATTCAGA TGGTCCTGGC 
ACTTCATCTG GAAGGAGGGC TAAATGTCGA GGCAGACGAC AGTCTCTCAA GTGTAATCCT 
GATGCTTGGA AAAAGCAATG CGAAGAGCTA CTGAGCCTCA TTTATGAACG GGAAGACTCA 
GAGCCATTTC GTCAGCCAGC AGATCCTCAA TCTTACCCAG TTCAACAGCA ACAAGAAGGG 
GAATCCTCAC AATCAGTTCC TCCAGATAGG CAAGACCCAT CTCTTTCTGA GGATTATCAA 
GATGGTATAG ACACACCTAT GGACTTCAGC ACTGTGAAAG AAACTTTGGA ATCAGGAAAT 
TATGATAGTC CTCTGGAATT TTATAAAGAT GTTCGCCAAA TATTCAGCAA CTCCAAAGCT 
TATACTTCTA ATAAAAAGTC AAGGATCTAT AGCATGACGC TGAGATTGTC AGCCTTATTT 
GAAAATCACA TTAAAAACAT CATCTCTGAC TATAAGTCAG CAATCCAGAG TCAGAAGAGG 
AGAAGACCAC GGTACAGAAA GCGTCTGAGA AGCAGCAGCA GCTCGTTATC TAGCAGCAGA 
GCTCCCAGTC CAAAAGGCAA ACAGAAGCAA ATGAAGTTAC AGCCTAAAAA TGACCAGAAT 
ACCTCAGTGG CTTATGCCAG GACTAGCTCT CCTTTTTCTT CTCCTGTTTC AGATGCTGCT 
GAAGGTGTTT CACTGTATCT ACTTGATGAT GAAGGGGATG GGCCTTTTTC TCCATCAAGT 
TTCAGTGGAT ATAGTAGAAG TGGAAATAGC CATGACCCAG GGAAAGCCAA ATCATTTCGT 
AATAGAGTTT TACCAGCTAA ACAAGATCAC TCCCTTGATG GACCTCTTAC AAATGTTGAT 
GGCAGAGAGC CCCGGACAGG AGCCAAAAGA AAACTGCTCA GTGCTTCAGA AGAAGATGAA 
AGCATGGGAG GAGAAGAGAA AGAAATGAAA GAAACAAAGG AGCAAGTACA TTTATCCTCC 
TCTGAGAGTG GAGAGTTAGG CTCCAGTTTA AGTTCAGAAA GTACCTCTGG CTCTGACTCT 
GACTCTGAAT CCACTTCCAG AACCGATCAA GATTATGTTG ATGGAGACCA TGACTATAGC 
AAATTCATAC AAACCAGACC TAAAAGGAAA CTCAGAAAAC AACACACCAA TGGAAAAAGA 
AACTGGAAGA CGAGAGGCAC AGGAGGAAGA GGCAGATGGG GAAGATGGGG AAGATGGAGC 
AGAGGAGGCA GAGGAAGAGG TGGCAGGGGT CGAGGGGGTC GAGGGCGAGG TGGAGGTGGG 
GGGAGAGGGA GAGGACGAGG GAGAGGAGGA AGAGGTGCCT CTAGAGGATC AAGCCGAGCC 
AAACGAGCCC GAGTTGCAGA TGATGAATTT GATACGATGT TTTCAGGACG TTTCAGTCGA 
CTTCCACGGA TTAAAACAAG AAACCAAGGC AGGAGAACTG TTTTATATAA TGATGATTCA 
GATAATGACA ACTTTGTGTC CACTGAGGAT CCTCTGAATC TTGGTACATC CAGATCAGGC 
AGAGTACGTA AAATGACCGA AAAAGCCAGG GTTAGCCATC TCATGGGATG GAATTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brwd3 ( NM_001081477.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001074946.1 Mus musculus bromodomain and WD repeat domain containing 3 (Brwd3), mRNA.

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CloneID	OMu02510
Clone ID Related Accession (Same CDS sequence)	NM_001081477.1 , NM_001081477.2
Accession Version	NM_001081477.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5400bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1553011200000
Organism	Mus musculus(house mouse)
Product	bromodomain and WD repeat-containing protein 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL731764.11 and BX000698.11. On Jul 28, 2018 this sequence version replaced NM_001081477.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGG CACCTACCCA GATCGAAGCC GAGCTGTATT ACCTGATCGC AAGGTTCTTG 
CAGTCCGGAC CCTGTAACAA ATCCGCTCAG GTGCTAGTGC AAGAGCTCGA AGAGCATCAG 
CTGATTCCGC GCCGCTTAGA CTGGGAAGGG AAAGAGCACC GAAGAAGCTT CGAGGATCTG 
GTGGCAGCCA ATGCACACAT TCCTCCAGAC TACCTCCTTA AAATTTGTGA GAGGATTGGC 
CCTTTATTAG ATAAGGAAAT CCCTCAGAGT GTTCCTGGGG TACAGACATT ACTAGGTGTC 
GGTCGGCAGT CTCTGTTACG GGATGCCAAA GACTGTAAGA GTACACTATG GAATGGGTCT 
GCTTTTGCAG CCCTCCACAG AGGCAGACCC CCAGAACTAC CTGTAAATTA TGTGAAACCT 
CCAAACGTGG TGAATATCAC CTCAGCCAGG CAATTAACCG GATGTAGTCG CTTCAGCCAT 
GTTTTCCCCT CATCTGCTTA CCAGCACATT AAGATGCATA AGAGAATTCT GGGACACTTA 
TCTTCTGTCT ACTGTGTAGC CTTTGATCGA AGCGGGAGGC GAATTTTTAC AGGTTCAGAT 
GACTGTTTGG TGAAAATCTG GGCAACAGAT GATGGACGCC TCCTTGCCAC ACTGCGAGGG 
CACTCTGCTG AAATCTCCGA CATGGCTGTT AATTATGAAA ATACTCTCAT TGCTGCAGGC 
AGCTGTGATA AAGTTGTGAG AGTATGGTGT CTTCGTACTT GTGCACCAGT TGCTGTCCTT 
CAGGGACATT CAGCTTCTAT TACTTCCATA CAGTTTTGTC CATCAACTAA AGGCACAACT 
AGATACCTCA CTTCTACTGG TGCTGATGGA ACAATTTGTT TCTGGCAATG GCATGTAAAA 
ACAATGAAGT TTAGAGATCG CCCAGTAAAA TTTACTGAGA GATCCAGACC TGGAGTTCAG 
ATATCTTGTT CATCTTTCAG TTCTGGTGGT ATGTTCATTA CAACAGGGAG CACTGATCAT 
GTAATTAGGA TATACTATTT GGGTTCTGAG ATACCTGAGA AAATTGCTGA ATTAGAATCA 
CATACGGACA AAGTTGTTGC TGTTCAGTTC TGTAACAACG GAGACAGCTT AAGGTTTGTT 
AGTGGAAGTA GAGACGGAAC AGCAAGAATA TGGCAATATC AACAACAAGA GTGGAAGAGC 
ATAGTGCTAG ATATGGCTAC TAAAATGAGT GGGAATAATT TGACATCAGC TGAAGACAAA 
GTCACTAAAC TAAAGGTGAC TATGGTGGCT TGGGATCGGT ATGATACCAC TGTTATTACT 
GCAGTGAACA ATTTCCTTTT GAAAGTTTGG AATTCTGTCA CAGGGCAGCT TCTTCATACT 
TTATCTGGGC ATGATGATGA AGTATTTGTT CTAGAAGCAC ATCCATTTGA TCAAAGGATC 
ATACTTTCAG CAGGTCATGA TGGGAACATT TTTATTTGGG ACCTTGACCG GGGGACCAAA 
ATACGAAATT ACTTTAATAT GATCGAAGGC CAAGGTCATG GTGCAGTATT TGATTGTAAA 
TTTTCACCAG ATGGAAACCA TTTTGCCTGT ACAGACTCTC ATGGGCATTT GTTGCTATTT 
GGCTTTGGAT GCAGTAAATA CTATGAAAAG ATTCCAGATC AGATGTTCTT CCATACCGAT 
TATCGGCCCC TCATCAGAGA TGCCAATAAC TATGTATTAG ATGAACAAAC TCAACAAGCT 
CCTCACCTCA TGCCTCCCCC ATTCTTGGTG GATGTCGATG GAAATCCTCA TCCTACAAAA 
TTTCAACGGC TGGTACCAGG ACGGGAAAAC TGTAAAGATG AACAACTTAT TCCACAGTTA 
GGATATGTGG CTAATGGTGA CGGTGAGGTG GTAGAACAGG TAATTGGGCA GCAAACCAAT 
GACCAAGAAG AAAGCATTCT TGATGGAATA ATCAGGGAGT TACAGAGAGA ACAAGATCTC 
AGATTAATTA ATGAAGGAGA TGTTCCACAT TTTCCAATTA ATAGATCATA TTCTGTTAAT 
GGTGCTCTGA GTAGTCCAAA TATGGACATA CCTTCTTCCC CAAATATTGG CCTTCGGCGT 
AGTGGCCAAA TTGAAGGTGT TCGGCAGATG CACAACAATG CTCCCCGGAG CCAGATGGCC 
ACTGAAAGAG ATCTCATGGC CTGGAGCCGA AGAGTGGTGG TGAATGAGCT AAATAGTGGC 
GTTAGTAGGG TACAAGAAGA ATGTCGAAAT GCAAAAGGTG ACCTAGAAGT TAGTCTTTAT 
ACTGTTGAAA AGAAGAAAAA GCCATCTTAT CCTATCCAAA GAAATGATTA TCAGCCCAGC 
TGTGGAAGGT CTTTACGAAG GACACAACGC AAGCGACAGC ATACTTACCT AACAAGGTCG 
AACATAGAGC ATAATTCACA GGCATCATCT CAAACTTCCG GTGTACAAGA AGATTCAGAC 
AGCTCTTCAG AGGAAGATGA AACTGTTGGC ACAAGTGATG CTTCCGTAGA AGACCCTGTT 
GTTGAGTGGC AAAGTGAGAG TTCTTCCAGC GATTCATCAA GTGAATATTC TGACTGGATA 
GCAGATGCTG GGATAAATTT GCAGCCTCCC AAAAGACAAA CAAGACAGGC AACACAGAAA 
ATATACAGCA GTTCTGAAGA TGAAAATTTG AAATTAGAAG ATAGGCAGAA GAAACCTAAA 
CAGACTAAAA AGAAGAAAGG GGGACTTGTT TCTATGGCAG GCGAACCCAA TGAAGAATGG 
CTTGCCCCTC AGTGGATCTT GGATACCATA CCACGACGTT CTCCTTTTGT TCCACAGATG 
GGAGATGAGC TTATTTACTT TCGACAAGGA CATGAAGCTT ATGTGCGAGC TGTAAGGAAA 
TCAAAAATCT ATAGTGTTAA TTTACAAAAA CAGCCATGGA ACAAAATGGA TCTCAGGGAG 
CAAGAATTTG TGAAGATTGT AGGAATCAAA TATGAGATTG GACCACCTAC GCTATGCTGC 
TTGAAACTTG CATTTTTGGA TCCAATTTCA GGCAAAATGA CTGGAGAGTC TTTTTCCATT 
AAGTATCATG ACATGCCAGA TGTCATTGAT TTCCTTGTGT TACATCAGTT TTATAATGAA 
GCCAAAGAAA GAAACTGGCA GATTGGTGAT AGATTCCGCA GTATAATAGA TGATGCCTGG 
TGGTTTGGGA CTGTGGAAAG TCAGCAGCCT TTTCAGCCAG AATATCCTGA TAGCTCCTTC 
CAGTGTTACA GTGTTCACTG GGACAATAAT GAAAGAGAAA AAATGAGCCC CTGGGATATG 
GAGCCGATTC CAGATGGAAC TGCTTTTCCA GATGAAGTTG GTGCTGGTAT TCCTGTGTCC 
CAGGAAGAAC TAACTGCATT GCTCTACAAA CCCCAGGAAG GAGAGTGGGG TGCTCATTCC 
AGAGATGAGG AGTGTGAACG AGTTATTCAG GGCATCAACA ACCTTCTTTC CCTGGATTTT 
GCCAGCCCTT TTGCTGTTCC AGTGGATCTC AGTGCCTACC CCTTGTATTG TACTGTAGTT 
GCTTATCCAA CTGACCTCAA TACCATCAAA CAAAGACTTG AAAATCGCTT TTACAGGAGA 
ATATCAGCAT TAATGTGGGA GGTACGCTAT ATTGAACACA ATGCTAGGAC TTTCAATGAG 
CCAGATAGTC CCATAGTTAA AGCAGCCAAG ATTGTGACTG ATGTCCTACT TCGGTTTATT 
GGGGATCAAA GCTGTTCTGA TATTTTGGAT ACTTACAATA AAATCAAAGC AGAAGATCCA 
GACAGCAGCG ATCTGGAGGA GGATTCAGAA ATGGTTGACT TAGATTCAGA TGGTCCTGGC 
ACTTCATCTG GAAGGAGGGC TAAATGTCGA GGCAGACGAC AGTCTCTCAA GTGTAATCCT 
GATGCTTGGA AAAAGCAATG CGAAGAGCTA CTGAGCCTCA TTTATGAACG GGAAGACTCA 
GAGCCATTTC GTCAGCCAGC AGATCCTCAA TCTTACCCAG TTCAACAGCA ACAAGAAGGG 
GAATCCTCAC AATCAGTTCC TCCAGATAGG CAAGACCCAT CTCTTTCTGA GGATTATCAA 
GATGGTATAG ACACACCTAT GGACTTCAGC ACTGTGAAAG AAACTTTGGA ATCAGGAAAT 
TATGATAGTC CTCTGGAATT TTATAAAGAT GTTCGCCAAA TATTCAGCAA CTCCAAAGCT 
TATACTTCTA ATAAAAAGTC AAGGATCTAT AGCATGACGC TGAGATTGTC AGCCTTATTT 
GAAAATCACA TTAAAAACAT CATCTCTGAC TATAAGTCAG CAATCCAGAG TCAGAAGAGG 
AGAAGACCAC GGTACAGAAA GCGTCTGAGA AGCAGCAGCA GCTCGTTATC TAGCAGCAGA 
GCTCCCAGTC CAAAAGGCAA ACAGAAGCAA ATGAAGTTAC AGCCTAAAAA TGACCAGAAT 
ACCTCAGTGG CTTATGCCAG GACTAGCTCT CCTTTTTCTT CTCCTGTTTC AGATGCTGCT 
GAAGGTGTTT CACTGTATCT ACTTGATGAT GAAGGGGATG GGCCTTTTTC TCCATCAAGT 
TTCAGTGGAT ATAGTAGAAG TGGAAATAGC CATGACCCAG GGAAAGCCAA ATCATTTCGT 
AATAGAGTTT TACCAGCTAA ACAAGATCAC TCCCTTGATG GACCTCTTAC AAATGTTGAT 
GGCAGAGAGC CCCGGACAGG AGCCAAAAGA AAACTGCTCA GTGCTTCAGA AGAAGATGAA 
AGCATGGGAG GAGAAGAGAA AGAAATGAAA GAAACAAAGG AGCAAGTACA TTTATCCTCC 
TCTGAGAGTG GAGAGTTAGG CTCCAGTTTA AGTTCAGAAA GTACCTCTGG CTCTGACTCT 
GACTCTGAAT CCACTTCCAG AACCGATCAA GATTATGTTG ATGGAGACCA TGACTATAGC 
AAATTCATAC AAACCAGACC TAAAAGGAAA CTCAGAAAAC AACACACCAA TGGAAAAAGA 
AACTGGAAGA CGAGAGGCAC AGGAGGAAGA GGCAGATGGG GAAGATGGGG AAGATGGAGC 
AGAGGAGGCA GAGGAAGAGG TGGCAGGGGT CGAGGGGGTC GAGGGCGAGG TGGAGGTGGG 
GGGAGAGGGA GAGGACGAGG GAGAGGAGGA AGAGGTGCCT CTAGAGGATC AAGCCGAGCC 
AAACGAGCCC GAGTTGCAGA TGATGAATTT GATACGATGT TTTCAGGACG TTTCAGTCGA 
CTTCCACGGA TTAAAACAAG AAACCAAGGC AGGAGAACTG TTTTATATAA TGATGATTCA 
GATAATGACA ACTTTGTGTC CACTGAGGAT CCTCTGAATC TTGGTACATC CAGATCAGGC 
AGAGTACGTA AAATGACCGA AAAAGCCAGG GTTAGCCATC TCATGGGATG GAATTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074946.1
CDS	218..5617
Translation	MAAAPTQIEAELYYLIARFLQSGPCNKSAQVLVQELEEHQLIPRRLDWEGKEHRRSFEDLVAANAHIPPDYLLKICERIGPLLDKEIPQSVPGVQTLLGVGRQSLLRDAKDCKSTLWNGSAFAALHRGRPPELPVNYVKPPNVVNITSARQLTGCSRFSHVFPSSAYQHIKMHKRILGHLSSVYCVAFDRSGRRIFTGSDDCLVKIWATDDGRLLATLRGHSAEISDMAVNYENTLIAAGSCDKVVRVWCLRTCAPVAVLQGHSASITSIQFCPSTKGTTRYLTSTGADGTICFWQWHVKTMKFRDRPVKFTERSRPGVQISCSSFSSGGMFITTGSTDHVIRIYYLGSEIPEKIAELESHTDKVVAVQFCNNGDSLRFVSGSRDGTARIWQYQQQEWKSIVLDMATKMSGNNLTSAEDKVTKLKVTMVAWDRYDTTVITAVNNFLLKVWNSVTGQLLHTLSGHDDEVFVLEAHPFDQRIILSAGHDGNIFIWDLDRGTKIRNYFNMIEGQGHGAVFDCKFSPDGNHFACTDSHGHLLLFGFGCSKYYEKIPDQMFFHTDYRPLIRDANNYVLDEQTQQAPHLMPPPFLVDVDGNPHPTKFQRLVPGRENCKDEQLIPQLGYVANGDGEVVEQVIGQQTNDQEESILDGIIRELQREQDLRLINEGDVPHFPINRSYSVNGALSSPNMDIPSSPNIGLRRSGQIEGVRQMHNNAPRSQMATERDLMAWSRRVVVNELNSGVSRVQEECRNAKGDLEVSLYTVEKKKKPSYPIQRNDYQPSCGRSLRRTQRKRQHTYLTRSNIEHNSQASSQTSGVQEDSDSSSEEDETVGTSDASVEDPVVEWQSESSSSDSSSEYSDWIADAGINLQPPKRQTRQATQKIYSSSEDENLKLEDRQKKPKQTKKKKGGLVSMAGEPNEEWLAPQWILDTIPRRSPFVPQMGDELIYFRQGHEAYVRAVRKSKIYSVNLQKQPWNKMDLREQEFVKIVGIKYEIGPPTLCCLKLAFLDPISGKMTGESFSIKYHDMPDVIDFLVLHQFYNEAKERNWQIGDRFRSIIDDAWWFGTVESQQPFQPEYPDSSFQCYSVHWDNNEREKMSPWDMEPIPDGTAFPDEVGAGIPVSQEELTALLYKPQEGEWGAHSRDEECERVIQGINNLLSLDFASPFAVPVDLSAYPLYCTVVAYPTDLNTIKQRLENRFYRRISALMWEVRYIEHNARTFNEPDSPIVKAAKIVTDVLLRFIGDQSCSDILDTYNKIKAEDPDSSDLEEDSEMVDLDSDGPGTSSGRRAKCRGRRQSLKCNPDAWKKQCEELLSLIYEREDSEPFRQPADPQSYPVQQQQEGESSQSVPPDRQDPSLSEDYQDGIDTPMDFSTVKETLESGNYDSPLEFYKDVRQIFSNSKAYTSNKKSRIYSMTLRLSALFENHIKNIISDYKSAIQSQKRRRPRYRKRLRSSSSSLSSSRAPSPKGKQKQMKLQPKNDQNTSVAYARTSSPFSSPVSDAAEGVSLYLLDDEGDGPFSPSSFSGYSRSGNSHDPGKAKSFRNRVLPAKQDHSLDGPLTNVDGREPRTGAKRKLLSASEEDESMGGEEKEMKETKEQVHLSSSESGELGSSLSSESTSGSDSDSESTSRTDQDYVDGDHDYSKFIQTRPKRKLRKQHTNGKRNWKTRGTGGRGRWGRWGRWSRGGRGRGGRGRGGRGRGGGGGRGRGRGRGGRGASRGSSRAKRARVADDEFDTMFSGRFSRLPRIKTRNQGRRTVLYNDDSDNDNFVSTEDPLNLGTSRSGRVRKMTEKARVSHLMGWNY

Target ORF information:

RefSeq Version	NM_001081477.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus bromodomain and WD repeat domain containing 3 (Brwd3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081477.2

ORF Insert Sequence:

ATGGCGGCGG CACCTACCCA GATCGAAGCC GAGCTGTATT ACCTGATCGC AAGGTTCTTG 
CAGTCCGGAC CCTGTAACAA ATCCGCTCAG GTGCTAGTGC AAGAGCTCGA AGAGCATCAG 
CTGATTCCGC GCCGCTTAGA CTGGGAAGGG AAAGAGCACC GAAGAAGCTT CGAGGATCTG 
GTGGCAGCCA ATGCACACAT TCCTCCAGAC TACCTCCTTA AAATTTGTGA GAGGATTGGC 
CCTTTATTAG ATAAGGAAAT CCCTCAGAGT GTTCCTGGGG TACAGACATT ACTAGGTGTC 
GGTCGGCAGT CTCTGTTACG GGATGCCAAA GACTGTAAGA GTACACTATG GAATGGGTCT 
GCTTTTGCAG CCCTCCACAG AGGCAGACCC CCAGAACTAC CTGTAAATTA TGTGAAACCT 
CCAAACGTGG TGAATATCAC CTCAGCCAGG CAATTAACCG GATGTAGTCG CTTCAGCCAT 
GTTTTCCCCT CATCTGCTTA CCAGCACATT AAGATGCATA AGAGAATTCT GGGACACTTA 
TCTTCTGTCT ACTGTGTAGC CTTTGATCGA AGCGGGAGGC GAATTTTTAC AGGTTCAGAT 
GACTGTTTGG TGAAAATCTG GGCAACAGAT GATGGACGCC TCCTTGCCAC ACTGCGAGGG 
CACTCTGCTG AAATCTCCGA CATGGCTGTT AATTATGAAA ATACTCTCAT TGCTGCAGGC 
AGCTGTGATA AAGTTGTGAG AGTATGGTGT CTTCGTACTT GTGCACCAGT TGCTGTCCTT 
CAGGGACATT CAGCTTCTAT TACTTCCATA CAGTTTTGTC CATCAACTAA AGGCACAACT 
AGATACCTCA CTTCTACTGG TGCTGATGGA ACAATTTGTT TCTGGCAATG GCATGTAAAA 
ACAATGAAGT TTAGAGATCG CCCAGTAAAA TTTACTGAGA GATCCAGACC TGGAGTTCAG 
ATATCTTGTT CATCTTTCAG TTCTGGTGGT ATGTTCATTA CAACAGGGAG CACTGATCAT 
GTAATTAGGA TATACTATTT GGGTTCTGAG ATACCTGAGA AAATTGCTGA ATTAGAATCA 
CATACGGACA AAGTTGTTGC TGTTCAGTTC TGTAACAACG GAGACAGCTT AAGGTTTGTT 
AGTGGAAGTA GAGACGGAAC AGCAAGAATA TGGCAATATC AACAACAAGA GTGGAAGAGC 
ATAGTGCTAG ATATGGCTAC TAAAATGAGT GGGAATAATT TGACATCAGC TGAAGACAAA 
GTCACTAAAC TAAAGGTGAC TATGGTGGCT TGGGATCGGT ATGATACCAC TGTTATTACT 
GCAGTGAACA ATTTCCTTTT GAAAGTTTGG AATTCTGTCA CAGGGCAGCT TCTTCATACT 
TTATCTGGGC ATGATGATGA AGTATTTGTT CTAGAAGCAC ATCCATTTGA TCAAAGGATC 
ATACTTTCAG CAGGTCATGA TGGGAACATT TTTATTTGGG ACCTTGACCG GGGGACCAAA 
ATACGAAATT ACTTTAATAT GATCGAAGGC CAAGGTCATG GTGCAGTATT TGATTGTAAA 
TTTTCACCAG ATGGAAACCA TTTTGCCTGT ACAGACTCTC ATGGGCATTT GTTGCTATTT 
GGCTTTGGAT GCAGTAAATA CTATGAAAAG ATTCCAGATC AGATGTTCTT CCATACCGAT 
TATCGGCCCC TCATCAGAGA TGCCAATAAC TATGTATTAG ATGAACAAAC TCAACAAGCT 
CCTCACCTCA TGCCTCCCCC ATTCTTGGTG GATGTCGATG GAAATCCTCA TCCTACAAAA 
TTTCAACGGC TGGTACCAGG ACGGGAAAAC TGTAAAGATG AACAACTTAT TCCACAGTTA 
GGATATGTGG CTAATGGTGA CGGTGAGGTG GTAGAACAGG TAATTGGGCA GCAAACCAAT 
GACCAAGAAG AAAGCATTCT TGATGGAATA ATCAGGGAGT TACAGAGAGA ACAAGATCTC 
AGATTAATTA ATGAAGGAGA TGTTCCACAT TTTCCAATTA ATAGATCATA TTCTGTTAAT 
GGTGCTCTGA GTAGTCCAAA TATGGACATA CCTTCTTCCC CAAATATTGG CCTTCGGCGT 
AGTGGCCAAA TTGAAGGTGT TCGGCAGATG CACAACAATG CTCCCCGGAG CCAGATGGCC 
ACTGAAAGAG ATCTCATGGC CTGGAGCCGA AGAGTGGTGG TGAATGAGCT AAATAGTGGC 
GTTAGTAGGG TACAAGAAGA ATGTCGAAAT GCAAAAGGTG ACCTAGAAGT TAGTCTTTAT 
ACTGTTGAAA AGAAGAAAAA GCCATCTTAT CCTATCCAAA GAAATGATTA TCAGCCCAGC 
TGTGGAAGGT CTTTACGAAG GACACAACGC AAGCGACAGC ATACTTACCT AACAAGGTCG 
AACATAGAGC ATAATTCACA GGCATCATCT CAAACTTCCG GTGTACAAGA AGATTCAGAC 
AGCTCTTCAG AGGAAGATGA AACTGTTGGC ACAAGTGATG CTTCCGTAGA AGACCCTGTT 
GTTGAGTGGC AAAGTGAGAG TTCTTCCAGC GATTCATCAA GTGAATATTC TGACTGGATA 
GCAGATGCTG GGATAAATTT GCAGCCTCCC AAAAGACAAA CAAGACAGGC AACACAGAAA 
ATATACAGCA GTTCTGAAGA TGAAAATTTG AAATTAGAAG ATAGGCAGAA GAAACCTAAA 
CAGACTAAAA AGAAGAAAGG GGGACTTGTT TCTATGGCAG GCGAACCCAA TGAAGAATGG 
CTTGCCCCTC AGTGGATCTT GGATACCATA CCACGACGTT CTCCTTTTGT TCCACAGATG 
GGAGATGAGC TTATTTACTT TCGACAAGGA CATGAAGCTT ATGTGCGAGC TGTAAGGAAA 
TCAAAAATCT ATAGTGTTAA TTTACAAAAA CAGCCATGGA ACAAAATGGA TCTCAGGGAG 
CAAGAATTTG TGAAGATTGT AGGAATCAAA TATGAGATTG GACCACCTAC GCTATGCTGC 
TTGAAACTTG CATTTTTGGA TCCAATTTCA GGCAAAATGA CTGGAGAGTC TTTTTCCATT 
AAGTATCATG ACATGCCAGA TGTCATTGAT TTCCTTGTGT TACATCAGTT TTATAATGAA 
GCCAAAGAAA GAAACTGGCA GATTGGTGAT AGATTCCGCA GTATAATAGA TGATGCCTGG 
TGGTTTGGGA CTGTGGAAAG TCAGCAGCCT TTTCAGCCAG AATATCCTGA TAGCTCCTTC 
CAGTGTTACA GTGTTCACTG GGACAATAAT GAAAGAGAAA AAATGAGCCC CTGGGATATG 
GAGCCGATTC CAGATGGAAC TGCTTTTCCA GATGAAGTTG GTGCTGGTAT TCCTGTGTCC 
CAGGAAGAAC TAACTGCATT GCTCTACAAA CCCCAGGAAG GAGAGTGGGG TGCTCATTCC 
AGAGATGAGG AGTGTGAACG AGTTATTCAG GGCATCAACA ACCTTCTTTC CCTGGATTTT 
GCCAGCCCTT TTGCTGTTCC AGTGGATCTC AGTGCCTACC CCTTGTATTG TACTGTAGTT 
GCTTATCCAA CTGACCTCAA TACCATCAAA CAAAGACTTG AAAATCGCTT TTACAGGAGA 
ATATCAGCAT TAATGTGGGA GGTACGCTAT ATTGAACACA ATGCTAGGAC TTTCAATGAG 
CCAGATAGTC CCATAGTTAA AGCAGCCAAG ATTGTGACTG ATGTCCTACT TCGGTTTATT 
GGGGATCAAA GCTGTTCTGA TATTTTGGAT ACTTACAATA AAATCAAAGC AGAAGATCCA 
GACAGCAGCG ATCTGGAGGA GGATTCAGAA ATGGTTGACT TAGATTCAGA TGGTCCTGGC 
ACTTCATCTG GAAGGAGGGC TAAATGTCGA GGCAGACGAC AGTCTCTCAA GTGTAATCCT 
GATGCTTGGA AAAAGCAATG CGAAGAGCTA CTGAGCCTCA TTTATGAACG GGAAGACTCA 
GAGCCATTTC GTCAGCCAGC AGATCCTCAA TCTTACCCAG TTCAACAGCA ACAAGAAGGG 
GAATCCTCAC AATCAGTTCC TCCAGATAGG CAAGACCCAT CTCTTTCTGA GGATTATCAA 
GATGGTATAG ACACACCTAT GGACTTCAGC ACTGTGAAAG AAACTTTGGA ATCAGGAAAT 
TATGATAGTC CTCTGGAATT TTATAAAGAT GTTCGCCAAA TATTCAGCAA CTCCAAAGCT 
TATACTTCTA ATAAAAAGTC AAGGATCTAT AGCATGACGC TGAGATTGTC AGCCTTATTT 
GAAAATCACA TTAAAAACAT CATCTCTGAC TATAAGTCAG CAATCCAGAG TCAGAAGAGG 
AGAAGACCAC GGTACAGAAA GCGTCTGAGA AGCAGCAGCA GCTCGTTATC TAGCAGCAGA 
GCTCCCAGTC CAAAAGGCAA ACAGAAGCAA ATGAAGTTAC AGCCTAAAAA TGACCAGAAT 
ACCTCAGTGG CTTATGCCAG GACTAGCTCT CCTTTTTCTT CTCCTGTTTC AGATGCTGCT 
GAAGGTGTTT CACTGTATCT ACTTGATGAT GAAGGGGATG GGCCTTTTTC TCCATCAAGT 
TTCAGTGGAT ATAGTAGAAG TGGAAATAGC CATGACCCAG GGAAAGCCAA ATCATTTCGT 
AATAGAGTTT TACCAGCTAA ACAAGATCAC TCCCTTGATG GACCTCTTAC AAATGTTGAT 
GGCAGAGAGC CCCGGACAGG AGCCAAAAGA AAACTGCTCA GTGCTTCAGA AGAAGATGAA 
AGCATGGGAG GAGAAGAGAA AGAAATGAAA GAAACAAAGG AGCAAGTACA TTTATCCTCC 
TCTGAGAGTG GAGAGTTAGG CTCCAGTTTA AGTTCAGAAA GTACCTCTGG CTCTGACTCT 
GACTCTGAAT CCACTTCCAG AACCGATCAA GATTATGTTG ATGGAGACCA TGACTATAGC 
AAATTCATAC AAACCAGACC TAAAAGGAAA CTCAGAAAAC AACACACCAA TGGAAAAAGA 
AACTGGAAGA CGAGAGGCAC AGGAGGAAGA GGCAGATGGG GAAGATGGGG AAGATGGAGC 
AGAGGAGGCA GAGGAAGAGG TGGCAGGGGT CGAGGGGGTC GAGGGCGAGG TGGAGGTGGG 
GGGAGAGGGA GAGGACGAGG GAGAGGAGGA AGAGGTGCCT CTAGAGGATC AAGCCGAGCC 
AAACGAGCCC GAGTTGCAGA TGATGAATTT GATACGATGT TTTCAGGACG TTTCAGTCGA 
CTTCCACGGA TTAAAACAAG AAACCAAGGC AGGAGAACTG TTTTATATAA TGATGATTCA 
GATAATGACA ACTTTGTGTC CACTGAGGAT CCTCTGAATC TTGGTACATC CAGATCAGGC 
AGAGTACGTA AAATGACCGA AAAAGCCAGG GTTAGCCATC TCATGGGATG GAATTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brwd3 ( XM_006528114.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006528177.1 Mus musculus bromodomain and WD repeat domain containing 3 (Brwd3), transcript variant X1, mRNA.

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CloneID	OMu56305
Clone ID Related Accession (Same CDS sequence)	XM_006528114.3
Accession Version	XM_006528114.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5241bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	bromodomain and WD repeat-containing protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006528114.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CACCTACCCA GATCGAAGCC GAGCTGTATT ACCTGATCGC AAGGTTCTTG 
CAGTCCGGAC CCTGTAACAA ATCCGCTCAG GTGCTAGTGC AAGAGCTCGA AGAGCATCAG 
CTGATTCCGC GCCGCTTAGA CTGGGAAGGG AAAGAGCACC GAAGAAGCTT CGAGGATCTG 
GTGGCAGCCA ATGCACACAT TCCTCCAGAC TACCTCCTTA AAATTTGTGA GAGGATTGGC 
CCTTTATTAG ATAAGGAAAT CCCTCAGAGT GTTCCTGGGG TACAGACATT ACTAGGTGTC 
GGTCGGCAGT CTCTGTTACG GGATGCCAAA GACTGTAAGA GTACACTATG GAATGGGTCT 
GCTTTTGCAG CCCTCCACAG AGGCAGACCC CCAGAACTAC CTGTAAATTA TGTGAAACCT 
CCAAACGTGG TGAATATCAC CTCAGCCAGG CAATTAACCG GATGTAGTCG CTTCAGCCAT 
GTTTTCCCCT CATCTGCTTA CCAGCACATT AAGATGCATA AGAGAATTCT GGGACACTTA 
TCTTCTGTCT ACTGTGTAGC CTTTGATCGA AGCGGGAGGC GAATTTTTAC AGGTTCAGAT 
GACTGTTTGG TGAAAATCTG GGCAACAGAT GATGGACGCC TCCTTGCCAC ACTGCGAGGG 
CACTCTGCTG AAATCTCCGA CATGGCTGTT AATTATGAAA ATACTCTCAT TGCTGCAGGC 
AGCTGTGATA AAGTTGTGAG AGTATGGTGT CTTCGTACTT GTGCACCAGT TGCTGTCCTT 
CAGGGACATT CAGCTTCTAT TACTTCCATA CAGTTTTGTC CATCAACTAA AGGCACAACT 
AGATACCTCA CTTCTACTGG TGCTGATGGA ACAATTTGTT TCTGGCAATG GCATGTAAAA 
ACAATGAAGT TTAGAGATCG CCCAGTAAAA TTTACTGAGA GATCCAGACC TGGAGTTCAG 
ATATCTTGTT CATCTTTCAG TTCTGGTGGT ATGTTCATTA CAACAGGGAG CACTGATCAT 
GTAATTAGGA TATACTATTT GGGTTCTGAG ATACCTGAGA AAATTGCTGA ATTAGAATCA 
CATACGGACA AAGTTGTTGC TGTTCAGTTC TGTAACAACG GAGACAGCTT AAGGTTTGTT 
AGTGGAAGTA GAGACGGAAC AGCAAGAATA TGGCAATATC AACAACAAGA GTGGAAGAGC 
ATAGTGCTAG ATATGGCTAC TAAAATGAGT GGGAATAATT TGACATCAGC TGAAGACAAA 
GTCACTAAAC TAAAGGTGAC TATGGTGGCT TGGGATCGGT ATGATACCAC TGTTATTACT 
GCAGTGAACA ATTTCCTTTT GAAAGTTTGG AATTCTGTCA CAGGGCAGCT TCTTCATACT 
TTATCTGGGC ATGATGATGA AGTATTTGTT CTAGAAGCAC ATCCATTTGA TCAAAGGATC 
ATACTTTCAG CAGGTCATGA TGGGAACATT TTTATTTGGG ACCTTGACCG GGGGACCAAA 
ATACGAAATT ACTTTAATAT GATCGAAGGC CAAGGTCATG GTGCAGTATT TGATTGTAAA 
TTTTCACCAG ATGGAAACCA TTTTGCCTGT ACAGACTCTC ATGGGCATTT GTTGCTATTT 
GGCTTTGGAT GCAGTAAATA CTATGAAAAG ATTCCAGATC AGATGTTCTT CCATACCGAT 
TATCGGCCCC TCATCAGAGA TGCCAATAAC TATGTATTAG ATGAACAAAC TCAACAAGCT 
CCTCACCTCA TGCCTCCCCC ATTCTTGGTG GATGTCGATG GAAATCCTCA TCCTACAAAA 
TTTCAACGGC TGGTACCAGG ACGGGAAAAC TGTAAAGATG AACAACTTAT TCCACAGTTA 
GGATATGTGG CTAATGGTGA CGGTGAGGTG GTAGAACAGG TAATTGGGCA GCAAACCAAT 
GACCAAGAAG AAAGCATTCT TGATGGAATA ATCAGGGAGT TACAGAGAGA ACAAGATCTC 
AGATTAATTA ATGAAGGAGA TGTTCCACAT TTTCCAATTA ATAGATCATA TTCTGTTAAT 
GGTGCTCTGA GTAGTCCAAA TATGGACATA CCTTCTTCCC CAAATATTGG CCTTCGGCGT 
AGTGGCCAAA TTGAAGGTGT TCGGCAGATG CACAACAATG CTCCCCGGAG CCAGATGGCC 
ACTGAAAGAG ATCTCATGGC CTGGAGCCGA AGAGTGGTGG TGAATGAGCT AAATAGTGGC 
GTTAGTAGGG TACAAGAAGA ATGTCGAAAT GCAAAAGGTG ACCTAGAAGT TAGTCTTTAT 
ACTGTTGAAA AGAAGAAAAA GCCATCTTAT CCTATCCAAA GAAATGATTA TCAGCCCAGC 
TGTGGAAGGT CTTTACGAAG GACACAACGC AAGCGACAGC ATACTTACCT AACAAGGTCG 
AACATAGAGC ATAATTCACA GGCATCATCT CAAACTTCCG GTGTACAAGA AGATTCAGAC 
AGCTCTTCAG AGGAAGATGA AACTGTTGGC ACAAGTGATG CTTCCGTAGA AGACCCTGTT 
GTTGAGTGGC AAAGTGAGAG TTCTTCCAGC GATTCATCAA GTGAATATTC TGACTGGATA 
GCAGATGCTG GGATAAATTT GCAGCCTCCC AAAAGACAAA CAAGACAGGC AACACAGAAA 
ATATACAGCA GTTCTGAAGA TGAAAATTTG AAATTAGAAG ATAGGCAGAA GAAACCTAAA 
CAGACTAAAA AGAAGAAAGG GGGACTTGTT TCTATGGCAG GCGAACCCAA TGAAGAATGG 
CTTGCCCCTC AGTGGATCTT GGATACCATA CCACGACGTT CTCCTTTTGT TCCACAGATG 
GGAGATGAGC TTATTTACTT TCGACAAGGA CATGAAGCTT ATGTGCGAGC TGTAAGGAAA 
TCAAAAATCT ATAGTGTTAA TTTACAAAAA CAGCCATGGA ACAAAATGGA TCTCAGGGAG 
CAAGAATTTG TGAAGATTGT AGGAATCAAA TATGAGATTG GACCACCTAC GCTATGCTGC 
TTGAAACTTG CATTTTTGGA TCCAATTTCA GGCAAAATGA CTGGAGAGTC TTTTTCCATT 
AAGTATCATG ACATGCCAGA TGTCATTGAT TTCCTTGTGT TACATCAGTT TTATAATGAA 
GCCAAAGAAA GAAACTGGCA GATTGGTGAT AGATTCCGCA GTATAATAGA TGATGCCTGG 
TGGTTTGGGA CTGTGGAAAG TCAGCAGCCT TTTCAGCCAG AATATCCTGA TAGCTCCTTC 
CAGTGTTACA GTGTTCACTG GGACAATAAT GAAAGAGAAA AAATGAGCCC CTGGGATATG 
GAGCCGATTC CAGATGGAAC TGCTTTTCCA GATGAAGTTG GTGCTGGTAT TCCTGTGTCC 
CAGGAAGAAC TAACTGCATT GCTCTACAAA CCCCAGGAAG GAGAGTGGGG TGCTCATTCC 
AGAGATGAGG AGTGTGAACG AGTTATTCAG GGCATCAACA ACCTTCTTTC CCTGGATTTT 
GCCAGCCCTT TTGCTGTTCC AGTGGATCTC AGTGCCTACC CCTTGTATTG TACTGTAGTT 
GCTTATCCAA CTGACCTCAA TACCATCAAA CAAAGACTTG AAAATCGCTT TTACAGGAGA 
ATATCAGCAT TAATGTGGGA GGTACGCTAT ATTGAACACA ATGCTAGGAC TTTCAATGAG 
CCAGATAGTC CCATAGTTAA AGCAGCCAAG ATTGTGACTG ATGTCCTACT TCGGTTTATT 
GGGGATCAAA GCTGTTCTGA TATTTTGGAT ACTTACAATA AAATCAAAGC AGAAGATCCA 
GACAGCAGCG ATCTGGAGGA GGATTCAGAA ATGGTTGACT TAGATTCAGA TGGTCCTGGC 
ACTTCATCTG GAAGGAGGGC TAAATGTCGA GGCAGACGAC AGTCTCTCAA GTGTAATCCT 
GATGCTTGGA AAAAGCAATG CGAAGAGCTA CTGAGCCTCA TTTATGAACG GGAAGACTCA 
GAGCCATTTC GTCAGCCAGC AGATCCTCAA TCTTACCCAG TTCAACAGCA ACAAGAAGGG 
GAATCCTCAC AATCAGTTCC TCCAGATAGG CAAGACCCAT CTCTTTCTGA GGATTATCAA 
GATGGTATAG ACACACCTAT GGACTTCAGC ACTGTGAAAG AAACTTTGGA ATCAGGAAAT 
TATGATAGTC CTCTGGAATT TTATAAAGAT GTTCGCCAAA TATTCAGCAA CTCCAAAGCT 
TATACTTCTA ATAAAAAGTC AAGGATCTAT AGCATGACGC TGAGATTGTC AGCCTTATTT 
GAAAATCACA TTAAAAACAT CATCTCTGAC TATAAGTCAG CAATCCAGAG TCAGAAGAGG 
AGAAGACCAC GGTACAGAAA GCGTCTGAGA AGCAGCAGCA GCTCGTTATC TAGCAGCAGA 
GCTCCCAGTC CAAAAGGCAA ACAGAAGCAA ATGAAGTTAC AGCCTAAAAA TGACCAGAAT 
ACCTCAGTGG CTTATGCCAG GACTAGCTCT CCTTTTTCTT CTCCTGATCA CTCCCTTGAT 
GGACCTCTTA CAAATGTTGA TGGCAGAGAG CCCCGGACAG GAGCCAAAAG AAAACTGCTC 
AGTGCTTCAG AAGAAGATGA AAGCATGGGA GGAGAAGAGA AAGAAATGAA AGAAACAAAG 
GAGCAAGTAC ATTTATCCTC CTCTGAGAGT GGAGAGTTAG GCTCCAGTTT AAGTTCAGAA 
AGTACCTCTG GCTCTGACTC TGACTCTGAA TCCACTTCCA GAACCGATCA AGATTATGTT 
GATGGAGACC ATGACTATAG CAAATTCATA CAAACCAGAC CTAAAAGGAA ACTCAGAAAA 
CAACACACCA ATGGAAAAAG AAACTGGAAG ACGAGAGGCA CAGGAGGAAG AGGCAGATGG 
GGAAGATGGG GAAGATGGAG CAGAGGAGGC AGAGGAAGAG GTGGCAGGGG TCGAGGGGGT 
CGAGGGCGAG GTGGAGGTGG GGGGAGAGGG AGAGGACGAG GGAGAGGAGG AAGAGGTGCC 
TCTAGAGGAT CAAGCCGAGC CAAACGAGCC CGAGTTGCAG ATGATGAATT TGATACGATG 
TTTTCAGGAC GTTTCAGTCG ACTTCCACGG ATTAAAACAA GAAACCAAGG CAGGAGAACT 
GTTTTATATA ATGATGATTC AGATAATGAC AACTTTGTGT CCACTGAGGA TCCTCTGAAT 
CTTGGTACAT CCAGATCAGG CAGAGTACGT AAAATGACCG AAAAAGCCAG GGTTAGCCAT 
CTCATGGGAT GGAATTATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006528177.1
CDS	234..5474
Translation	MAAAPTQIEAELYYLIARFLQSGPCNKSAQVLVQELEEHQLIPRRLDWEGKEHRRSFEDLVAANAHIPPDYLLKICERIGPLLDKEIPQSVPGVQTLLGVGRQSLLRDAKDCKSTLWNGSAFAALHRGRPPELPVNYVKPPNVVNITSARQLTGCSRFSHVFPSSAYQHIKMHKRILGHLSSVYCVAFDRSGRRIFTGSDDCLVKIWATDDGRLLATLRGHSAEISDMAVNYENTLIAAGSCDKVVRVWCLRTCAPVAVLQGHSASITSIQFCPSTKGTTRYLTSTGADGTICFWQWHVKTMKFRDRPVKFTERSRPGVQISCSSFSSGGMFITTGSTDHVIRIYYLGSEIPEKIAELESHTDKVVAVQFCNNGDSLRFVSGSRDGTARIWQYQQQEWKSIVLDMATKMSGNNLTSAEDKVTKLKVTMVAWDRYDTTVITAVNNFLLKVWNSVTGQLLHTLSGHDDEVFVLEAHPFDQRIILSAGHDGNIFIWDLDRGTKIRNYFNMIEGQGHGAVFDCKFSPDGNHFACTDSHGHLLLFGFGCSKYYEKIPDQMFFHTDYRPLIRDANNYVLDEQTQQAPHLMPPPFLVDVDGNPHPTKFQRLVPGRENCKDEQLIPQLGYVANGDGEVVEQVIGQQTNDQEESILDGIIRELQREQDLRLINEGDVPHFPINRSYSVNGALSSPNMDIPSSPNIGLRRSGQIEGVRQMHNNAPRSQMATERDLMAWSRRVVVNELNSGVSRVQEECRNAKGDLEVSLYTVEKKKKPSYPIQRNDYQPSCGRSLRRTQRKRQHTYLTRSNIEHNSQASSQTSGVQEDSDSSSEEDETVGTSDASVEDPVVEWQSESSSSDSSSEYSDWIADAGINLQPPKRQTRQATQKIYSSSEDENLKLEDRQKKPKQTKKKKGGLVSMAGEPNEEWLAPQWILDTIPRRSPFVPQMGDELIYFRQGHEAYVRAVRKSKIYSVNLQKQPWNKMDLREQEFVKIVGIKYEIGPPTLCCLKLAFLDPISGKMTGESFSIKYHDMPDVIDFLVLHQFYNEAKERNWQIGDRFRSIIDDAWWFGTVESQQPFQPEYPDSSFQCYSVHWDNNEREKMSPWDMEPIPDGTAFPDEVGAGIPVSQEELTALLYKPQEGEWGAHSRDEECERVIQGINNLLSLDFASPFAVPVDLSAYPLYCTVVAYPTDLNTIKQRLENRFYRRISALMWEVRYIEHNARTFNEPDSPIVKAAKIVTDVLLRFIGDQSCSDILDTYNKIKAEDPDSSDLEEDSEMVDLDSDGPGTSSGRRAKCRGRRQSLKCNPDAWKKQCEELLSLIYEREDSEPFRQPADPQSYPVQQQQEGESSQSVPPDRQDPSLSEDYQDGIDTPMDFSTVKETLESGNYDSPLEFYKDVRQIFSNSKAYTSNKKSRIYSMTLRLSALFENHIKNIISDYKSAIQSQKRRRPRYRKRLRSSSSSLSSSRAPSPKGKQKQMKLQPKNDQNTSVAYARTSSPFSSPDHSLDGPLTNVDGREPRTGAKRKLLSASEEDESMGGEEKEMKETKEQVHLSSSESGELGSSLSSESTSGSDSDSESTSRTDQDYVDGDHDYSKFIQTRPKRKLRKQHTNGKRNWKTRGTGGRGRWGRWGRWSRGGRGRGGRGRGGRGRGGGGGRGRGRGRGGRGASRGSSRAKRARVADDEFDTMFSGRFSRLPRIKTRNQGRRTVLYNDDSDNDNFVSTEDPLNLGTSRSGRVRKMTEKARVSHLMGWNY

Target ORF information:

RefSeq Version	XM_006528114.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus bromodomain and WD repeat domain containing 3 (Brwd3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006528114.3

ORF Insert Sequence:

ATGGCGGCGG CACCTACCCA GATCGAAGCC GAGCTGTATT ACCTGATCGC AAGGTTCTTG 
CAGTCCGGAC CCTGTAACAA ATCCGCTCAG GTGCTAGTGC AAGAGCTCGA AGAGCATCAG 
CTGATTCCGC GCCGCTTAGA CTGGGAAGGG AAAGAGCACC GAAGAAGCTT CGAGGATCTG 
GTGGCAGCCA ATGCACACAT TCCTCCAGAC TACCTCCTTA AAATTTGTGA GAGGATTGGC 
CCTTTATTAG ATAAGGAAAT CCCTCAGAGT GTTCCTGGGG TACAGACATT ACTAGGTGTC 
GGTCGGCAGT CTCTGTTACG GGATGCCAAA GACTGTAAGA GTACACTATG GAATGGGTCT 
GCTTTTGCAG CCCTCCACAG AGGCAGACCC CCAGAACTAC CTGTAAATTA TGTGAAACCT 
CCAAACGTGG TGAATATCAC CTCAGCCAGG CAATTAACCG GATGTAGTCG CTTCAGCCAT 
GTTTTCCCCT CATCTGCTTA CCAGCACATT AAGATGCATA AGAGAATTCT GGGACACTTA 
TCTTCTGTCT ACTGTGTAGC CTTTGATCGA AGCGGGAGGC GAATTTTTAC AGGTTCAGAT 
GACTGTTTGG TGAAAATCTG GGCAACAGAT GATGGACGCC TCCTTGCCAC ACTGCGAGGG 
CACTCTGCTG AAATCTCCGA CATGGCTGTT AATTATGAAA ATACTCTCAT TGCTGCAGGC 
AGCTGTGATA AAGTTGTGAG AGTATGGTGT CTTCGTACTT GTGCACCAGT TGCTGTCCTT 
CAGGGACATT CAGCTTCTAT TACTTCCATA CAGTTTTGTC CATCAACTAA AGGCACAACT 
AGATACCTCA CTTCTACTGG TGCTGATGGA ACAATTTGTT TCTGGCAATG GCATGTAAAA 
ACAATGAAGT TTAGAGATCG CCCAGTAAAA TTTACTGAGA GATCCAGACC TGGAGTTCAG 
ATATCTTGTT CATCTTTCAG TTCTGGTGGT ATGTTCATTA CAACAGGGAG CACTGATCAT 
GTAATTAGGA TATACTATTT GGGTTCTGAG ATACCTGAGA AAATTGCTGA ATTAGAATCA 
CATACGGACA AAGTTGTTGC TGTTCAGTTC TGTAACAACG GAGACAGCTT AAGGTTTGTT 
AGTGGAAGTA GAGACGGAAC AGCAAGAATA TGGCAATATC AACAACAAGA GTGGAAGAGC 
ATAGTGCTAG ATATGGCTAC TAAAATGAGT GGGAATAATT TGACATCAGC TGAAGACAAA 
GTCACTAAAC TAAAGGTGAC TATGGTGGCT TGGGATCGGT ATGATACCAC TGTTATTACT 
GCAGTGAACA ATTTCCTTTT GAAAGTTTGG AATTCTGTCA CAGGGCAGCT TCTTCATACT 
TTATCTGGGC ATGATGATGA AGTATTTGTT CTAGAAGCAC ATCCATTTGA TCAAAGGATC 
ATACTTTCAG CAGGTCATGA TGGGAACATT TTTATTTGGG ACCTTGACCG GGGGACCAAA 
ATACGAAATT ACTTTAATAT GATCGAAGGC CAAGGTCATG GTGCAGTATT TGATTGTAAA 
TTTTCACCAG ATGGAAACCA TTTTGCCTGT ACAGACTCTC ATGGGCATTT GTTGCTATTT 
GGCTTTGGAT GCAGTAAATA CTATGAAAAG ATTCCAGATC AGATGTTCTT CCATACCGAT 
TATCGGCCCC TCATCAGAGA TGCCAATAAC TATGTATTAG ATGAACAAAC TCAACAAGCT 
CCTCACCTCA TGCCTCCCCC ATTCTTGGTG GATGTCGATG GAAATCCTCA TCCTACAAAA 
TTTCAACGGC TGGTACCAGG ACGGGAAAAC TGTAAAGATG AACAACTTAT TCCACAGTTA 
GGATATGTGG CTAATGGTGA CGGTGAGGTG GTAGAACAGG TAATTGGGCA GCAAACCAAT 
GACCAAGAAG AAAGCATTCT TGATGGAATA ATCAGGGAGT TACAGAGAGA ACAAGATCTC 
AGATTAATTA ATGAAGGAGA TGTTCCACAT TTTCCAATTA ATAGATCATA TTCTGTTAAT 
GGTGCTCTGA GTAGTCCAAA TATGGACATA CCTTCTTCCC CAAATATTGG CCTTCGGCGT 
AGTGGCCAAA TTGAAGGTGT TCGGCAGATG CACAACAATG CTCCCCGGAG CCAGATGGCC 
ACTGAAAGAG ATCTCATGGC CTGGAGCCGA AGAGTGGTGG TGAATGAGCT AAATAGTGGC 
GTTAGTAGGG TACAAGAAGA ATGTCGAAAT GCAAAAGGTG ACCTAGAAGT TAGTCTTTAT 
ACTGTTGAAA AGAAGAAAAA GCCATCTTAT CCTATCCAAA GAAATGATTA TCAGCCCAGC 
TGTGGAAGGT CTTTACGAAG GACACAACGC AAGCGACAGC ATACTTACCT AACAAGGTCG 
AACATAGAGC ATAATTCACA GGCATCATCT CAAACTTCCG GTGTACAAGA AGATTCAGAC 
AGCTCTTCAG AGGAAGATGA AACTGTTGGC ACAAGTGATG CTTCCGTAGA AGACCCTGTT 
GTTGAGTGGC AAAGTGAGAG TTCTTCCAGC GATTCATCAA GTGAATATTC TGACTGGATA 
GCAGATGCTG GGATAAATTT GCAGCCTCCC AAAAGACAAA CAAGACAGGC AACACAGAAA 
ATATACAGCA GTTCTGAAGA TGAAAATTTG AAATTAGAAG ATAGGCAGAA GAAACCTAAA 
CAGACTAAAA AGAAGAAAGG GGGACTTGTT TCTATGGCAG GCGAACCCAA TGAAGAATGG 
CTTGCCCCTC AGTGGATCTT GGATACCATA CCACGACGTT CTCCTTTTGT TCCACAGATG 
GGAGATGAGC TTATTTACTT TCGACAAGGA CATGAAGCTT ATGTGCGAGC TGTAAGGAAA 
TCAAAAATCT ATAGTGTTAA TTTACAAAAA CAGCCATGGA ACAAAATGGA TCTCAGGGAG 
CAAGAATTTG TGAAGATTGT AGGAATCAAA TATGAGATTG GACCACCTAC GCTATGCTGC 
TTGAAACTTG CATTTTTGGA TCCAATTTCA GGCAAAATGA CTGGAGAGTC TTTTTCCATT 
AAGTATCATG ACATGCCAGA TGTCATTGAT TTCCTTGTGT TACATCAGTT TTATAATGAA 
GCCAAAGAAA GAAACTGGCA GATTGGTGAT AGATTCCGCA GTATAATAGA TGATGCCTGG 
TGGTTTGGGA CTGTGGAAAG TCAGCAGCCT TTTCAGCCAG AATATCCTGA TAGCTCCTTC 
CAGTGTTACA GTGTTCACTG GGACAATAAT GAAAGAGAAA AAATGAGCCC CTGGGATATG 
GAGCCGATTC CAGATGGAAC TGCTTTTCCA GATGAAGTTG GTGCTGGTAT TCCTGTGTCC 
CAGGAAGAAC TAACTGCATT GCTCTACAAA CCCCAGGAAG GAGAGTGGGG TGCTCATTCC 
AGAGATGAGG AGTGTGAACG AGTTATTCAG GGCATCAACA ACCTTCTTTC CCTGGATTTT 
GCCAGCCCTT TTGCTGTTCC AGTGGATCTC AGTGCCTACC CCTTGTATTG TACTGTAGTT 
GCTTATCCAA CTGACCTCAA TACCATCAAA CAAAGACTTG AAAATCGCTT TTACAGGAGA 
ATATCAGCAT TAATGTGGGA GGTACGCTAT ATTGAACACA ATGCTAGGAC TTTCAATGAG 
CCAGATAGTC CCATAGTTAA AGCAGCCAAG ATTGTGACTG ATGTCCTACT TCGGTTTATT 
GGGGATCAAA GCTGTTCTGA TATTTTGGAT ACTTACAATA AAATCAAAGC AGAAGATCCA 
GACAGCAGCG ATCTGGAGGA GGATTCAGAA ATGGTTGACT TAGATTCAGA TGGTCCTGGC 
ACTTCATCTG GAAGGAGGGC TAAATGTCGA GGCAGACGAC AGTCTCTCAA GTGTAATCCT 
GATGCTTGGA AAAAGCAATG CGAAGAGCTA CTGAGCCTCA TTTATGAACG GGAAGACTCA 
GAGCCATTTC GTCAGCCAGC AGATCCTCAA TCTTACCCAG TTCAACAGCA ACAAGAAGGG 
GAATCCTCAC AATCAGTTCC TCCAGATAGG CAAGACCCAT CTCTTTCTGA GGATTATCAA 
GATGGTATAG ACACACCTAT GGACTTCAGC ACTGTGAAAG AAACTTTGGA ATCAGGAAAT 
TATGATAGTC CTCTGGAATT TTATAAAGAT GTTCGCCAAA TATTCAGCAA CTCCAAAGCT 
TATACTTCTA ATAAAAAGTC AAGGATCTAT AGCATGACGC TGAGATTGTC AGCCTTATTT 
GAAAATCACA TTAAAAACAT CATCTCTGAC TATAAGTCAG CAATCCAGAG TCAGAAGAGG 
AGAAGACCAC GGTACAGAAA GCGTCTGAGA AGCAGCAGCA GCTCGTTATC TAGCAGCAGA 
GCTCCCAGTC CAAAAGGCAA ACAGAAGCAA ATGAAGTTAC AGCCTAAAAA TGACCAGAAT 
ACCTCAGTGG CTTATGCCAG GACTAGCTCT CCTTTTTCTT CTCCTGATCA CTCCCTTGAT 
GGACCTCTTA CAAATGTTGA TGGCAGAGAG CCCCGGACAG GAGCCAAAAG AAAACTGCTC 
AGTGCTTCAG AAGAAGATGA AAGCATGGGA GGAGAAGAGA AAGAAATGAA AGAAACAAAG 
GAGCAAGTAC ATTTATCCTC CTCTGAGAGT GGAGAGTTAG GCTCCAGTTT AAGTTCAGAA 
AGTACCTCTG GCTCTGACTC TGACTCTGAA TCCACTTCCA GAACCGATCA AGATTATGTT 
GATGGAGACC ATGACTATAG CAAATTCATA CAAACCAGAC CTAAAAGGAA ACTCAGAAAA 
CAACACACCA ATGGAAAAAG AAACTGGAAG ACGAGAGGCA CAGGAGGAAG AGGCAGATGG 
GGAAGATGGG GAAGATGGAG CAGAGGAGGC AGAGGAAGAG GTGGCAGGGG TCGAGGGGGT 
CGAGGGCGAG GTGGAGGTGG GGGGAGAGGG AGAGGACGAG GGAGAGGAGG AAGAGGTGCC 
TCTAGAGGAT CAAGCCGAGC CAAACGAGCC CGAGTTGCAG ATGATGAATT TGATACGATG 
TTTTCAGGAC GTTTCAGTCG ACTTCCACGG ATTAAAACAA GAAACCAAGG CAGGAGAACT 
GTTTTATATA ATGATGATTC AGATAATGAC AACTTTGTGT CCACTGAGGA TCCTCTGAAT 
CTTGGTACAT CCAGATCAGG CAGAGTACGT AAAATGACCG AAAAAGCCAG GGTTAGCCAT 
CTCATGGGAT GGAATTATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Epigenetic functions of smchd1 repress gene clusters on the inactive X chromosome and on autosomes.
Molecular and cellular biology33(16)3150-65(2013 Aug)
Gendrel AV,Tang YA,Suzuki M,Godwin J,Nesterova TB,Greally JM,Heard E,Brockdorff N

Phenotypic annotation of the mouse X chromosome.
Genome research20(8)1154-64(2010 Aug)
Cox BJ,Vollmer M,Tamplin O,Lu M,Biechele S,Gertsenstein M,van Campenhout C,Floss T,Kühn R,Wurst W,Lickert H,Rossant J

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

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Brwd3 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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