dlc1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	dlc1
Entrez Gene ID	100488460
Full Name	DLC1 Rho GTPase activating protein
Synonyms	rhogap7
General protein information	Preferred Names DLC1 Rho GTPase activating protein Names rho GTPase-activating protein 7 deleted in liver cancer 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031895414.1	XP_031751274.1	rho GTPase-activating protein 7 isoform X1
XM_031895415.1	XP_031751275.1	rho GTPase-activating protein 7 isoform X2
XM_012955669.2	XP_012811123.1	rho GTPase-activating protein 7

Pathways from BioSystems

Homologies

Homo sapiens (human)	DLC1	NP_872584.2
Pan troglodytes (chimpanzee)	DLC1	XP_001139210.2
Mus musculus (house mouse)	Dlc1	NP_001181869.1
Rattus norvegicus (Norway rat)	Dlc1	NP_001120918.1
Gallus gallus (chicken)	DLC1	XP_420693.3
Macaca mulatta (Rhesus monkey)	DLC1	XP_001092830.1
Canis lupus familiaris (dog)	DLC1	NP_001138543.1
Bos taurus (cattle)	DLC1	NP_001095963.1
Xenopus tropicalis (tropical clawed frog)	dlc1	XP_002934721.2
Danio rerio (zebrafish)	LOC567965	XP_005159917.1

Gene Ontology

The following dlc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dlc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa04706	XM_012955669.2 Latest version!	Xenopus tropicalis DLC1 Rho GTPase activating protein (dlc1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa49751	XM_031895414.1 Latest version!	Xenopus tropicalis DLC1 Rho GTPase activating protein (dlc1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa49752	XM_031895415.1 Latest version!	Xenopus tropicalis DLC1 Rho GTPase activating protein (dlc1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dlc1 ( XM_012955669.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811123.1 Xenopus tropicalis DLC1 Rho GTPase activating protein (dlc1), mRNA.

Price & Availability↑

CloneID	OXa04706
Clone ID Related Accession (Same CDS sequence)	XM_012955669.2
Accession Version	XM_012955669.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho GTPase-activating protein 7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012955669.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCAACG AGAAGCTGGA CAGAATGATC CTCACACAAA TAGAGGCCAA GGAAGCATGT 
GACTGGCTAC GGGCAGCGGG ATTCCCACAG TATGCGCAGC TCTACGAAGA TCTTCTTTTT 
CCTATTGACA TCTCTTCCGT AAAGAAGGAC CATGACTTCT TGGACAGGGA TGCAATCGAG 
GCGCTCTGCA GGCGACTGAA TACTTTAAAC AAGTGTGCTG TGATGAAATT AGAAATTAGC 
CCAAATAGGA AAAGGAGTGA AGAGTCTGAT GAAGATGAAC CATGTGCAAT CAGTGGTAAA 
TGGACATTTC AAAGGGACAG CAAGAGGTGG TCTCGACTGG AAGAATTTGA AGTCTTTCCT 
CTTACTCAAG GACCAAAAGC TCTACTGTCA GACGCACACC AGCTGGGAAA TGCAATCAGC 
CAGGACAGTA TTCTTACTGA CCTTAGTGAG CACCAAGATG TAGTATCAAT TACCAGTACC 
AGCAGCGAAA GTCCCAGAGC ACACCTTCCC AGTGATGCCA GCACTACAAG AACAAATTCT 
GTCACCAGCA TTTGTTCATC TGGAAATTTT ATTGCAAACG ATGACTCATT TGGGAGCCTT 
CCATCGCCAA CAGAGCTTTC CACTTTCAAT TTCACCTCAA AGGCTAACCA AAAGAACTCA 
AAGTCTAGAA CAAAGAGCCT CCTTAAGAGG ATGGAAAGTC TGAAAATCAA AAGCTCTAAC 
AGTAAAAAGA AGTCACATTC CAAACTGGGT CTGATTATTA GTGAACCTAT CCTCCAAGAA 
GCGGTGGATG ATGATAAGCT AAAGCAACTT AACTGCGTAG AAATTTCCTC CCTTAATGGC 
AACCATATCA ACGCACCCAA GGTGCGGAAA AGGAGCACAT CAAACTCAAC TCAAACAAGT 
AGCAGTAGTA GCCAATCAGA CACTAGCAGT GCCGTAAGCA CTCCCAGTCC TGTAACAAGG 
ACAAGGAGTT TAAGTGCCTG CCATAAAAGA GTAGGTATGT ATTTAGAGGG CTTTGACCCT 
TTCAATCAGT CAACATTTAG TGATGTCCTC GAACAGAACT ATAAAAACCA GAGGACTTTT 
GGTGAGGATG CTGTGTTTTT TATTCCTGAA GACCACAAAC CTGGCACCTT TCCCAAGGCA 
CTTTCAAATG GCAATTTGTC TCCTTCTGAC AGTAGCCCCT CTGTCACCTG GAGAACTGGG 
AGTTTCCATG GACATGACAA CCTTAGCTTT AGAAGGGAAA ACAGCATCAG CAGCTGCAAA 
AGTTCGGTTA GCAGCCAGAG ACGGAATTCT TGTTGTTCTG AAGGAAGCCG CTTAAGTATT 
TATGACAATG TACCAGGAAC TCTTCTGTAT TCCAGTAACA GCGACCTAGC TGATCTGGAA 
AACGAAGACA TCTTTCCAGA ACTTGATGAT ATATTATACC ATGTGAAGGG GATGCAAAGG 
ATTGTGAATC AGTGGTCAGA GAAATTTTAT GATGAGGGAG ATTCTGATTC TGCTTTAGAT 
TCTGTATCTC CCTGCCCTTC CTCCCCCAAG CAGATACACT TGGATATTGA CAACGACTGC 
AATGCTCTTA GTGATCTCGA TAGCACAGGA AATTCATTTA ATGATTGTGA AGAAGCAACC 
GTAATCCAGG CGAGGAGAGA CTCCGGAGTT GGCGCTTCTC TGACGCGAAC TAACAGACAC 
CGGTTCAGAT GGCACAGCTT CCAGAGCTCC CACCGTCCCA GTTTAAGTTC TGCTTCATTG 
CAGATGAACT GCCAGTCCGT GACTCAGATC AACTTGCTTC AGAAGTATTC CCTCTTAAAG 
CTAACGGCTC TCCTGGAAAA ATATACCCCT TCCAACAAGC ACGGGTTCAG CTGGGCCGTG 
CCAAAATTTA TGAAACGCGT GAAAGTTCCA GACTACAAGG ACCGAAACGT GTTTGGCGTT 
CCTCTTGCAA TAAATGTACA GCGCACTGGT CAGCCATTGC CACAAAGCAT CCAGCTGGCC 
ATGCGTTATC TACGTAGTCA GTGCTTGGAT CAGGTGGGGC TTTTCAGAAA ATCAGGCGTC 
AAGTCCAGAA TCCTGGCTTT GCGGGAGATA AGTGAAAATA ACAACGACAG CTTGACCTAC 
GAGGGACAGT CTGCATATGA TGTGGCGGAC ATGTTAAAGC AGTATTTCAG AGACCTGCCA 
GAGCCACTTT TAACCAGCAA GCTTTCCGAA ACTTTCCTGC AAATCTACCA GTATGTCCCC 
AAGGAGCAGC GCCTGCAGGC AATCAAGGCG GCCATTATGC TGTTGCCTGA TGAAAACAGG 
GAAGTCCTGC AAACCCTTTT ATACTTCCTG AGTGATGTTG CAGCAGCTGT AGATGAGAAC 
CAGATGACCC CCACCAACCT CGCTGTCTGC TTGGCTCCTT CCTTGTTCCA CCTTAACACC 
TTGAAAAGAG AGAACTCCTC TCCCAGGGTT ATGCACAGAA AGCAGAGTCT AGGAAAGCCT 
GATCAGAAGG ACCTGAATGA AAACCTTGCT GCCACTCAGG GTTTGGCTCA CATGATTGCC 
GAGTGCAAGA AACTTTTCAA GATTCCTGAA GAAATGAGCA AGTGCCGTAA TTCATACATG 
GAGCACGACC TGAGACCCGT GTGCCTGGAG GAGCTTGTTA ATGGCAATGC AGAGGGGCTG 
TGTGACTATC AGTCGTACCT TCAAGAGAAC ATTGACGTCT TGCTCAAGGA TGCTAAAGAC 
AAATTCAAAG GCTGGGTCAA CTGTTCGACA TCAGAGCAAG CAGATCTCTC TTACAAAAAG 
GTGTGCGAAG GCCCTCCTCT TCGATTGTGG AAAGCCACCG TTGACATCCC TGCTCAGCCA 
GAAGATGTTC TTCACCGCTT GCTGAGAGAG CAGCACCTGT GGGATGAAGA TCTCCTGGAT 
TCCAAAGTTA TTGAGAGTTT GGATGACCAG ACTGATATTT ATCAATATGT GCAAAATTAC 
ATGGCGCCCC ATCCAGCCAG GGACTTTGTT GTATTAAGAA CATGGAGAAC CAACTTAGCG 
AAAGGCGCTT GTGTTCTTTC GTGCACTTCA GTGGATCACG ATCGTGCTCC TCAGCTCGGC 
GTCAGAGTAA ATGTTCTCAT GTCCAGATAC CTCATTGAAC CAGGCAGTTC GGGAAAATCC 
AAGCTCACAT ACCTGTGCAG AGCTGATTTG AGGGGCCATA TGCCAGAGTG GTACAATAAG 
GTTTTTGGGC ACGTGTGCGC AGCTGAAGTT GTAAGGATCA GAGACTCTTT TATGAACCAA 
CAATCAGAAA GCAAAGATGG AAAGTCCAGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811123.1
CDS	475..3747
Translation	MCNEKLDRMILTQIEAKEACDWLRAAGFPQYAQLYEDLLFPIDISSVKKDHDFLDRDAIEALCRRLNTLNKCAVMKLEISPNRKRSEESDEDEPCAISGKWTFQRDSKRWSRLEEFEVFPLTQGPKALLSDAHQLGNAISQDSILTDLSEHQDVVSITSTSSESPRAHLPSDASTTRTNSVTSICSSGNFIANDDSFGSLPSPTELSTFNFTSKANQKNSKSRTKSLLKRMESLKIKSSNSKKKSHSKLGLIISEPILQEAVDDDKLKQLNCVEISSLNGNHINAPKVRKRSTSNSTQTSSSSSQSDTSSAVSTPSPVTRTRSLSACHKRVGMYLEGFDPFNQSTFSDVLEQNYKNQRTFGEDAVFFIPEDHKPGTFPKALSNGNLSPSDSSPSVTWRTGSFHGHDNLSFRRENSISSCKSSVSSQRRNSCCSEGSRLSIYDNVPGTLLYSSNSDLADLENEDIFPELDDILYHVKGMQRIVNQWSEKFYDEGDSDSALDSVSPCPSSPKQIHLDIDNDCNALSDLDSTGNSFNDCEEATVIQARRDSGVGASLTRTNRHRFRWHSFQSSHRPSLSSASLQMNCQSVTQINLLQKYSLLKLTALLEKYTPSNKHGFSWAVPKFMKRVKVPDYKDRNVFGVPLAINVQRTGQPLPQSIQLAMRYLRSQCLDQVGLFRKSGVKSRILALREISENNNDSLTYEGQSAYDVADMLKQYFRDLPEPLLTSKLSETFLQIYQYVPKEQRLQAIKAAIMLLPDENREVLQTLLYFLSDVAAAVDENQMTPTNLAVCLAPSLFHLNTLKRENSSPRVMHRKQSLGKPDQKDLNENLAATQGLAHMIAECKKLFKIPEEMSKCRNSYMEHDLRPVCLEELVNGNAEGLCDYQSYLQENIDVLLKDAKDKFKGWVNCSTSEQADLSYKKVCEGPPLRLWKATVDIPAQPEDVLHRLLREQHLWDEDLLDSKVIESLDDQTDIYQYVQNYMAPHPARDFVVLRTWRTNLAKGACVLSCTSVDHDRAPQLGVRVNVLMSRYLIEPGSSGKSKLTYLCRADLRGHMPEWYNKVFGHVCAAEVVRIRDSFMNQQSESKDGKSR

Target ORF information:

RefSeq Version	XM_012955669.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DLC1 Rho GTPase activating protein (dlc1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012955669.2

ORF Insert Sequence:

ATGTGCAACG AGAAGCTGGA CAGAATGATC CTCACACAAA TAGAGGCCAA GGAAGCATGT 
GACTGGCTAC GGGCAGCGGG ATTCCCACAG TATGCGCAGC TCTACGAAGA TCTTCTTTTT 
CCTATTGACA TCTCTTCCGT AAAGAAGGAC CATGACTTCT TGGACAGGGA TGCAATCGAG 
GCGCTCTGCA GGCGACTGAA TACTTTAAAC AAGTGTGCTG TGATGAAATT AGAAATTAGC 
CCAAATAGGA AAAGGAGTGA AGAGTCTGAT GAAGATGAAC CATGTGCAAT CAGTGGTAAA 
TGGACATTTC AAAGGGACAG CAAGAGGTGG TCTCGACTGG AAGAATTTGA AGTCTTTCCT 
CTTACTCAAG GACCAAAAGC TCTACTGTCA GACGCACACC AGCTGGGAAA TGCAATCAGC 
CAGGACAGTA TTCTTACTGA CCTTAGTGAG CACCAAGATG TAGTATCAAT TACCAGTACC 
AGCAGCGAAA GTCCCAGAGC ACACCTTCCC AGTGATGCCA GCACTACAAG AACAAATTCT 
GTCACCAGCA TTTGTTCATC TGGAAATTTT ATTGCAAACG ATGACTCATT TGGGAGCCTT 
CCATCGCCAA CAGAGCTTTC CACTTTCAAT TTCACCTCAA AGGCTAACCA AAAGAACTCA 
AAGTCTAGAA CAAAGAGCCT CCTTAAGAGG ATGGAAAGTC TGAAAATCAA AAGCTCTAAC 
AGTAAAAAGA AGTCACATTC CAAACTGGGT CTGATTATTA GTGAACCTAT CCTCCAAGAA 
GCGGTGGATG ATGATAAGCT AAAGCAACTT AACTGCGTAG AAATTTCCTC CCTTAATGGC 
AACCATATCA ACGCACCCAA GGTGCGGAAA AGGAGCACAT CAAACTCAAC TCAAACAAGT 
AGCAGTAGTA GCCAATCAGA CACTAGCAGT GCCGTAAGCA CTCCCAGTCC TGTAACAAGG 
ACAAGGAGTT TAAGTGCCTG CCATAAAAGA GTAGGTATGT ATTTAGAGGG CTTTGACCCT 
TTCAATCAGT CAACATTTAG TGATGTCCTC GAACAGAACT ATAAAAACCA GAGGACTTTT 
GGTGAGGATG CTGTGTTTTT TATTCCTGAA GACCACAAAC CTGGCACCTT TCCCAAGGCA 
CTTTCAAATG GCAATTTGTC TCCTTCTGAC AGTAGCCCCT CTGTCACCTG GAGAACTGGG 
AGTTTCCATG GACATGACAA CCTTAGCTTT AGAAGGGAAA ACAGCATCAG CAGCTGCAAA 
AGTTCGGTTA GCAGCCAGAG ACGGAATTCT TGTTGTTCTG AAGGAAGCCG CTTAAGTATT 
TATGACAATG TACCAGGAAC TCTTCTGTAT TCCAGTAACA GCGACCTAGC TGATCTGGAA 
AACGAAGACA TCTTTCCAGA ACTTGATGAT ATATTATACC ATGTGAAGGG GATGCAAAGG 
ATTGTGAATC AGTGGTCAGA GAAATTTTAT GATGAGGGAG ATTCTGATTC TGCTTTAGAT 
TCTGTATCTC CCTGCCCTTC CTCCCCCAAG CAGATACACT TGGATATTGA CAACGACTGC 
AATGCTCTTA GTGATCTCGA TAGCACAGGA AATTCATTTA ATGATTGTGA AGAAGCAACC 
GTAATCCAGG CGAGGAGAGA CTCCGGAGTT GGCGCTTCTC TGACGCGAAC TAACAGACAC 
CGGTTCAGAT GGCACAGCTT CCAGAGCTCC CACCGTCCCA GTTTAAGTTC TGCTTCATTG 
CAGATGAACT GCCAGTCCGT GACTCAGATC AACTTGCTTC AGAAGTATTC CCTCTTAAAG 
CTAACGGCTC TCCTGGAAAA ATATACCCCT TCCAACAAGC ACGGGTTCAG CTGGGCCGTG 
CCAAAATTTA TGAAACGCGT GAAAGTTCCA GACTACAAGG ACCGAAACGT GTTTGGCGTT 
CCTCTTGCAA TAAATGTACA GCGCACTGGT CAGCCATTGC CACAAAGCAT CCAGCTGGCC 
ATGCGTTATC TACGTAGTCA GTGCTTGGAT CAGGTGGGGC TTTTCAGAAA ATCAGGCGTC 
AAGTCCAGAA TCCTGGCTTT GCGGGAGATA AGTGAAAATA ACAACGACAG CTTGACCTAC 
GAGGGACAGT CTGCATATGA TGTGGCGGAC ATGTTAAAGC AGTATTTCAG AGACCTGCCA 
GAGCCACTTT TAACCAGCAA GCTTTCCGAA ACTTTCCTGC AAATCTACCA GTATGTCCCC 
AAGGAGCAGC GCCTGCAGGC AATCAAGGCG GCCATTATGC TGTTGCCTGA TGAAAACAGG 
GAAGTCCTGC AAACCCTTTT ATACTTCCTG AGTGATGTTG CAGCAGCTGT AGATGAGAAC 
CAGATGACCC CCACCAACCT CGCTGTCTGC TTGGCTCCTT CCTTGTTCCA CCTTAACACC 
TTGAAAAGAG AGAACTCCTC TCCCAGGGTT ATGCACAGAA AGCAGAGTCT AGGAAAGCCT 
GATCAGAAGG ACCTGAATGA AAACCTTGCT GCCACTCAGG GTTTGGCTCA CATGATTGCC 
GAGTGCAAGA AACTTTTCAA GATTCCTGAA GAAATGAGCA AGTGCCGTAA TTCATACATG 
GAGCACGACC TGAGACCCGT GTGCCTGGAG GAGCTTGTTA ATGGCAATGC AGAGGGGCTG 
TGTGACTATC AGTCGTACCT TCAAGAGAAC ATTGACGTCT TGCTCAAGGA TGCTAAAGAC 
AAATTCAAAG GCTGGGTCAA CTGTTCGACA TCAGAGCAAG CAGATCTCTC TTACAAAAAG 
GTGTGCGAAG GCCCTCCTCT TCGATTGTGG AAAGCCACCG TTGACATCCC TGCTCAGCCA 
GAAGATGTTC TTCACCGCTT GCTGAGAGAG CAGCACCTGT GGGATGAAGA TCTCCTGGAT 
TCCAAAGTTA TTGAGAGTTT GGATGACCAG ACTGATATTT ATCAATATGT GCAAAATTAC 
ATGGCGCCCC ATCCAGCCAG GGACTTTGTT GTATTAAGAA CATGGAGAAC CAACTTAGCG 
AAAGGCGCTT GTGTTCTTTC GTGCACTTCA GTGGATCACG ATCGTGCTCC TCAGCTCGGC 
GTCAGAGTAA ATGTTCTCAT GTCCAGATAC CTCATTGAAC CAGGCAGTTC GGGAAAATCC 
AAGCTCACAT ACCTGTGCAG AGCTGATTTG AGGGGCCATA TGCCAGAGTG GTACAATAAG 
GTTTTTGGGC ACGTGTGCGC AGCTGAAGTT GTAAGGATCA GAGACTCTTT TATGAACCAA 
CAATCAGAAA GCAAAGATGG AAAGTCCAGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dlc1 ( XM_031895414.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031751274.1 Xenopus tropicalis DLC1 Rho GTPase activating protein (dlc1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa49751
Clone ID Related Accession (Same CDS sequence)	XM_031895414.1
Accession Version	XM_031895414.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho GTPase-activating protein 7 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGC CACTAAGAAC CCCACTAAGG AGATCATTCA GTGATCACAT CAGAAATTCC 
ACTGCCCGAG CTTTGGATGT GATCTGGAAA AATGCAAGAG AAAAACGTCT GGCAGAAATA 
GAGGCCAAGG AAGCATGTGA CTGGCTACGG GCAGCGGGAT TCCCACAGTA TGCGCAGCTC 
TACGAAGATC TTCTTTTTCC TATTGACATC TCTTCCGTAA AGAAGGACCA TGACTTCTTG 
GACAGGGATG CAATCGAGGC GCTCTGCAGG CGACTGAATA CTTTAAACAA GTGTGCTGTG 
ATGAAATTAG AAATTAGCCC AAATAGGAAA AGGAGTGAAG AGTCTGATGA AGATGAACCA 
TGTGCAATCA GTGGTAAATG GACATTTCAA AGGGACAGCA AGAGGTGGTC TCGACTGGAA 
GAATTTGAAG TCTTTCCTCT TACTCAAGGA CCAAAAGCTC TACTGTCAGA CGCACACCAG 
CTGGGAAATG CAATCAGCCA GGACAGTATT CTTACTGACC TTAGTGAGCA CCAAGATGTA 
GTATCAATTA CCAGTACCAG CAGCGAAAGT CCCAGAGCAC ACCTTCCCAG TGATGCCAGC 
ACTACAAGAA CAAATTCTGT CACCAGCATT TGTTCATCTG GAAATTTTAT TGCAAACGAT 
GACTCATTTG GGAGCCTTCC ATCGCCAACA GAGCTTTCCA CTTTCAATTT CACCTCAAAG 
GCTAACCAAA AGAACTCAAA GTCTAGAACA AAGAGCCTCC TTAAGAGGAT GGAAAGTCTG 
AAAATCAAAA GCTCTAACAG TAAAAAGAAG TCACATTCCA AACTGGGTCT GATTATTAGT 
GAACCTATCC TCCAAGAAGC GGTGGATGAT GATAAGCTAA AGCAACTTAA CTGCGTAGAA 
ATTTCCTCCC TTAATGGCAA CCATATCAAC GCACCCAAGG TGCGGAAAAG GAGCACATCA 
AACTCAACTC AAACAAGTAG CAGTAGTAGC CAATCAGACA CTAGCAGTGC CGTAAGCACT 
CCCAGTCCTG TAACAAGGAC AAGGAGTTTA AGTGCCTGCC ATAAAAGAGT AGGTATGTAT 
TTAGAGGGCT TTGACCCTTT CAATCAGTCA ACATTTAGTG ATGTCCTCGA ACAGAACTAT 
AAAAACCAGA GGACTTTTGG TGAGGATGCT GTGTTTTTTA TTCCTGAAGA CCACAAACCT 
GGCACCTTTC CCAAGGCACT TTCAAATGGC AATTTGTCTC CTTCTGACAG TAGCCCCTCT 
GTCACCTGGA GAACTGGGAG TTTCCATGGA CATGACAACC TTAGCTTTAG AAGGGAAAAC 
AGCATCAGCA GCTGCAAAAG TTCGGTTAGC AGCCAGAGAC GGAATTCTTG TTGTTCTGAA 
GGAAGCCGCT TAAGTATTTA TGACAATGTA CCAGGAACTC TTCTGTATTC CAGTAACAGC 
GACCTAGCTG ATCTGGAAAA CGAAGACATC TTTCCAGAAC TTGATGATAT ATTATACCAT 
GTGAAGGGGA TGCAAAGGAT TGTGAATCAG TGGTCAGAGA AATTTTATGA TGAGGGAGAT 
TCTGATTCTG CTTTAGATTC TGTATCTCCC TGCCCTTCCT CCCCCAAGCA GATACACTTG 
GATATTGACA ACGACTGCAA TGCTCTTAGT GATCTCGATA GCACAGGAAA TTCATTTAAT 
GATTGTGAAG AAGCAACCGT AATCCAGGCG AGGAGAGACT CCGGAGTTGG CGCTTCTCTG 
ACGCGAACTA ACAGACACCG GTTCAGATGG CACAGCTTCC AGAGCTCCCA CCGTCCCAGT 
TTAAGTTCTG CTTCATTGCA GATGAACTGC CAGTCCGTGA CTCAGATCAA CTTGCTTCAG 
AAGTATTCCC TCTTAAAGCT AACGGCTCTC CTGGAAAAAT ATACCCCTTC CAACAAGCAC 
GGGTTCAGCT GGGCCGTGCC AAAATTTATG AAACGCGTGA AAGTTCCAGA CTACAAGGAC 
CGAAACGTGT TTGGCGTTCC TCTTGCAATA AATGTACAGC GCACTGGTCA GCCATTGCCA 
CAAAGCATCC AGCTGGCCAT GCGTTACCTA CGTAGTCAGT GCTTGGATCA GGTGGGGCTT 
TTCAGAAAAT CAGGCGTCAA GTCCAGAATC CTGGCTTTGC GGGAGATAAG TGAAAATAAC 
AACGACAGCT TGACCTACGA GGGACAGTCT GCATATGATG TGGCGGACAT GTTAAAGCAG 
TATTTCAGAG ACCTGCCAGA GCCACTTTTA ACCAGCAAGC TTTCCGAAAC TTTCCTGCAA 
ATCTACCAGT ATGTCCCCAA GGAGCAGCGC CTGCAGGCAA TCAAGGCGGC CATTATGCTG 
TTGCCTGATG AAAACAGGGA AGTCCTGCAA ACCCTTTTAT ACTTCCTGAG TGATGTTGCA 
GCAGCTGTAG ATGAGAACCA GATGACCCCC ACCAACCTCG CTGTCTGCTT GGCTCCTTCC 
TTGTTCCACC TTAACACCTT GAAAAGAGAG AACTCCTCTC CCAGGGTTAT GCACAGAAAG 
CAGAGTCTAG GAAAGCCTGA TCAGAAGGAC CTGAATGAAA ACCTTGCTGC CACTCAGGGT 
TTGGCTCACA TGATTGCCGA GTGCAAGAAA CTTTTCAAGA TTCCTGAAGA AATGAGCAAG 
TGCCGTAATT CATACATGGA GCACGACCTG AGACCCGTGT GCCTGGAGGA GCTTGTTAAT 
GGCAATGCAG AGGGGCTGTG TGACTATCAG TCGTACCTTC AAGAGAACAT TGACGTCTTG 
CTCAAGGATG CTAAAGACAA ATTCAAAGGC TGGGTCAACT GTTCGACATC AGAGCAAGCA 
GATCTCTCTT ACAAAAAGGT GTGCGAAGGC CCTCCTCTTC GATTGTGGAA AGCCACCGTT 
GACATCCCTG CTCAGCCAGA AGATGTTCTT CACCGCTTGC TGAGAGAGCA GCACCTGTGG 
GATGAAGATC TCCTGGATTC CAAAGTTATT GAGAGTTTGG ATGACCAGAC TGATATTTAT 
CAATATGTGC AAAATTACAT GGCGCCCCAT CCAGCCAGGG ACTTTGTTGT ATTAAGAACA 
TGGAGAACCA ACTTAGCGAA AGGCGCTTGT GTTCTTTCGT GCACTTCAGT GGATCACGAT 
CGTGCTCCTC AGCTCGGCGT CAGAGTAAAT GTTCTCATGT CCAGATACCT CATTGAACCA 
GGCAGTTCGG GAAAATCCAA GCTCACATAC CTGTGCAGAG CTGATTTGAG GGGCCATATG 
CCAGAGTGGT ACAATAAGGT TTTTGGGCAC GTGTGCGCAG CTGAAGTTGT AAGGATCAGA 
GACTCTTTTA TGAACCAACA ATCAGAAAGC AAAGATGGAA AGTCCAGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031751274.1
CDS	158..3508
Translation	MEKPLRTPLRRSFSDHIRNSTARALDVIWKNAREKRLAEIEAKEACDWLRAAGFPQYAQLYEDLLFPIDISSVKKDHDFLDRDAIEALCRRLNTLNKCAVMKLEISPNRKRSEESDEDEPCAISGKWTFQRDSKRWSRLEEFEVFPLTQGPKALLSDAHQLGNAISQDSILTDLSEHQDVVSITSTSSESPRAHLPSDASTTRTNSVTSICSSGNFIANDDSFGSLPSPTELSTFNFTSKANQKNSKSRTKSLLKRMESLKIKSSNSKKKSHSKLGLIISEPILQEAVDDDKLKQLNCVEISSLNGNHINAPKVRKRSTSNSTQTSSSSSQSDTSSAVSTPSPVTRTRSLSACHKRVGMYLEGFDPFNQSTFSDVLEQNYKNQRTFGEDAVFFIPEDHKPGTFPKALSNGNLSPSDSSPSVTWRTGSFHGHDNLSFRRENSISSCKSSVSSQRRNSCCSEGSRLSIYDNVPGTLLYSSNSDLADLENEDIFPELDDILYHVKGMQRIVNQWSEKFYDEGDSDSALDSVSPCPSSPKQIHLDIDNDCNALSDLDSTGNSFNDCEEATVIQARRDSGVGASLTRTNRHRFRWHSFQSSHRPSLSSASLQMNCQSVTQINLLQKYSLLKLTALLEKYTPSNKHGFSWAVPKFMKRVKVPDYKDRNVFGVPLAINVQRTGQPLPQSIQLAMRYLRSQCLDQVGLFRKSGVKSRILALREISENNNDSLTYEGQSAYDVADMLKQYFRDLPEPLLTSKLSETFLQIYQYVPKEQRLQAIKAAIMLLPDENREVLQTLLYFLSDVAAAVDENQMTPTNLAVCLAPSLFHLNTLKRENSSPRVMHRKQSLGKPDQKDLNENLAATQGLAHMIAECKKLFKIPEEMSKCRNSYMEHDLRPVCLEELVNGNAEGLCDYQSYLQENIDVLLKDAKDKFKGWVNCSTSEQADLSYKKVCEGPPLRLWKATVDIPAQPEDVLHRLLREQHLWDEDLLDSKVIESLDDQTDIYQYVQNYMAPHPARDFVVLRTWRTNLAKGACVLSCTSVDHDRAPQLGVRVNVLMSRYLIEPGSSGKSKLTYLCRADLRGHMPEWYNKVFGHVCAAEVVRIRDSFMNQQSESKDGKSR

Target ORF information:

RefSeq Version	XM_031895414.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DLC1 Rho GTPase activating protein (dlc1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031895414.1

ORF Insert Sequence:

ATGGAGAAGC CACTAAGAAC CCCACTAAGG AGATCATTCA GTGATCACAT CAGAAATTCC 
ACTGCCCGAG CTTTGGATGT GATCTGGAAA AATGCAAGAG AAAAACGTCT GGCAGAAATA 
GAGGCCAAGG AAGCATGTGA CTGGCTACGG GCAGCGGGAT TCCCACAGTA TGCGCAGCTC 
TACGAAGATC TTCTTTTTCC TATTGACATC TCTTCCGTAA AGAAGGACCA TGACTTCTTG 
GACAGGGATG CAATCGAGGC GCTCTGCAGG CGACTGAATA CTTTAAACAA GTGTGCTGTG 
ATGAAATTAG AAATTAGCCC AAATAGGAAA AGGAGTGAAG AGTCTGATGA AGATGAACCA 
TGTGCAATCA GTGGTAAATG GACATTTCAA AGGGACAGCA AGAGGTGGTC TCGACTGGAA 
GAATTTGAAG TCTTTCCTCT TACTCAAGGA CCAAAAGCTC TACTGTCAGA CGCACACCAG 
CTGGGAAATG CAATCAGCCA GGACAGTATT CTTACTGACC TTAGTGAGCA CCAAGATGTA 
GTATCAATTA CCAGTACCAG CAGCGAAAGT CCCAGAGCAC ACCTTCCCAG TGATGCCAGC 
ACTACAAGAA CAAATTCTGT CACCAGCATT TGTTCATCTG GAAATTTTAT TGCAAACGAT 
GACTCATTTG GGAGCCTTCC ATCGCCAACA GAGCTTTCCA CTTTCAATTT CACCTCAAAG 
GCTAACCAAA AGAACTCAAA GTCTAGAACA AAGAGCCTCC TTAAGAGGAT GGAAAGTCTG 
AAAATCAAAA GCTCTAACAG TAAAAAGAAG TCACATTCCA AACTGGGTCT GATTATTAGT 
GAACCTATCC TCCAAGAAGC GGTGGATGAT GATAAGCTAA AGCAACTTAA CTGCGTAGAA 
ATTTCCTCCC TTAATGGCAA CCATATCAAC GCACCCAAGG TGCGGAAAAG GAGCACATCA 
AACTCAACTC AAACAAGTAG CAGTAGTAGC CAATCAGACA CTAGCAGTGC CGTAAGCACT 
CCCAGTCCTG TAACAAGGAC AAGGAGTTTA AGTGCCTGCC ATAAAAGAGT AGGTATGTAT 
TTAGAGGGCT TTGACCCTTT CAATCAGTCA ACATTTAGTG ATGTCCTCGA ACAGAACTAT 
AAAAACCAGA GGACTTTTGG TGAGGATGCT GTGTTTTTTA TTCCTGAAGA CCACAAACCT 
GGCACCTTTC CCAAGGCACT TTCAAATGGC AATTTGTCTC CTTCTGACAG TAGCCCCTCT 
GTCACCTGGA GAACTGGGAG TTTCCATGGA CATGACAACC TTAGCTTTAG AAGGGAAAAC 
AGCATCAGCA GCTGCAAAAG TTCGGTTAGC AGCCAGAGAC GGAATTCTTG TTGTTCTGAA 
GGAAGCCGCT TAAGTATTTA TGACAATGTA CCAGGAACTC TTCTGTATTC CAGTAACAGC 
GACCTAGCTG ATCTGGAAAA CGAAGACATC TTTCCAGAAC TTGATGATAT ATTATACCAT 
GTGAAGGGGA TGCAAAGGAT TGTGAATCAG TGGTCAGAGA AATTTTATGA TGAGGGAGAT 
TCTGATTCTG CTTTAGATTC TGTATCTCCC TGCCCTTCCT CCCCCAAGCA GATACACTTG 
GATATTGACA ACGACTGCAA TGCTCTTAGT GATCTCGATA GCACAGGAAA TTCATTTAAT 
GATTGTGAAG AAGCAACCGT AATCCAGGCG AGGAGAGACT CCGGAGTTGG CGCTTCTCTG 
ACGCGAACTA ACAGACACCG GTTCAGATGG CACAGCTTCC AGAGCTCCCA CCGTCCCAGT 
TTAAGTTCTG CTTCATTGCA GATGAACTGC CAGTCCGTGA CTCAGATCAA CTTGCTTCAG 
AAGTATTCCC TCTTAAAGCT AACGGCTCTC CTGGAAAAAT ATACCCCTTC CAACAAGCAC 
GGGTTCAGCT GGGCCGTGCC AAAATTTATG AAACGCGTGA AAGTTCCAGA CTACAAGGAC 
CGAAACGTGT TTGGCGTTCC TCTTGCAATA AATGTACAGC GCACTGGTCA GCCATTGCCA 
CAAAGCATCC AGCTGGCCAT GCGTTACCTA CGTAGTCAGT GCTTGGATCA GGTGGGGCTT 
TTCAGAAAAT CAGGCGTCAA GTCCAGAATC CTGGCTTTGC GGGAGATAAG TGAAAATAAC 
AACGACAGCT TGACCTACGA GGGACAGTCT GCATATGATG TGGCGGACAT GTTAAAGCAG 
TATTTCAGAG ACCTGCCAGA GCCACTTTTA ACCAGCAAGC TTTCCGAAAC TTTCCTGCAA 
ATCTACCAGT ATGTCCCCAA GGAGCAGCGC CTGCAGGCAA TCAAGGCGGC CATTATGCTG 
TTGCCTGATG AAAACAGGGA AGTCCTGCAA ACCCTTTTAT ACTTCCTGAG TGATGTTGCA 
GCAGCTGTAG ATGAGAACCA GATGACCCCC ACCAACCTCG CTGTCTGCTT GGCTCCTTCC 
TTGTTCCACC TTAACACCTT GAAAAGAGAG AACTCCTCTC CCAGGGTTAT GCACAGAAAG 
CAGAGTCTAG GAAAGCCTGA TCAGAAGGAC CTGAATGAAA ACCTTGCTGC CACTCAGGGT 
TTGGCTCACA TGATTGCCGA GTGCAAGAAA CTTTTCAAGA TTCCTGAAGA AATGAGCAAG 
TGCCGTAATT CATACATGGA GCACGACCTG AGACCCGTGT GCCTGGAGGA GCTTGTTAAT 
GGCAATGCAG AGGGGCTGTG TGACTATCAG TCGTACCTTC AAGAGAACAT TGACGTCTTG 
CTCAAGGATG CTAAAGACAA ATTCAAAGGC TGGGTCAACT GTTCGACATC AGAGCAAGCA 
GATCTCTCTT ACAAAAAGGT GTGCGAAGGC CCTCCTCTTC GATTGTGGAA AGCCACCGTT 
GACATCCCTG CTCAGCCAGA AGATGTTCTT CACCGCTTGC TGAGAGAGCA GCACCTGTGG 
GATGAAGATC TCCTGGATTC CAAAGTTATT GAGAGTTTGG ATGACCAGAC TGATATTTAT 
CAATATGTGC AAAATTACAT GGCGCCCCAT CCAGCCAGGG ACTTTGTTGT ATTAAGAACA 
TGGAGAACCA ACTTAGCGAA AGGCGCTTGT GTTCTTTCGT GCACTTCAGT GGATCACGAT 
CGTGCTCCTC AGCTCGGCGT CAGAGTAAAT GTTCTCATGT CCAGATACCT CATTGAACCA 
GGCAGTTCGG GAAAATCCAA GCTCACATAC CTGTGCAGAG CTGATTTGAG GGGCCATATG 
CCAGAGTGGT ACAATAAGGT TTTTGGGCAC GTGTGCGCAG CTGAAGTTGT AAGGATCAGA 
GACTCTTTTA TGAACCAACA ATCAGAAAGC AAAGATGGAA AGTCCAGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dlc1 ( XM_031895415.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031751275.1 Xenopus tropicalis DLC1 Rho GTPase activating protein (dlc1), transcript variant X2, mRNA.

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CloneID	OXa49752
Clone ID Related Accession (Same CDS sequence)	XM_031895415.1
Accession Version	XM_031895415.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho GTPase-activating protein 7 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCAACG AGAAGCTGGA CAGAATGATC CTCACACAAA TAGAGGCCAA GGAAGCATGT 
GACTGGCTAC GGGCAGCGGG ATTCCCACAG TATGCGCAGC TCTACGAAGA TCTTCTTTTT 
CCTATTGACA TCTCTTCCGT AAAGAAGGAC CATGACTTCT TGGACAGGGA TGCAATCGAG 
GCGCTCTGCA GGCGACTGAA TACTTTAAAC AAGTGTGCTG TGATGAAATT AGAAATTAGC 
CCAAATAGGA AAAGGAGTGA AGAGTCTGAT GAAGATGAAC CATGTGCAAT CAGTGGTAAA 
TGGACATTTC AAAGGGACAG CAAGAGGTGG TCTCGACTGG AAGAATTTGA AGTCTTTCCT 
CTTACTCAAG GACCAAAAGC TCTACTGTCA GACGCACACC AGCTGGGAAA TGCAATCAGC 
CAGGACAGTA TTCTTACTGA CCTTAGTGAG CACCAAGATG TAGTATCAAT TACCAGTACC 
AGCAGCGAAA GTCCCAGAGC ACACCTTCCC AGTGATGCCA GCACTACAAG AACAAATTCT 
GTCACCAGCA TTTGTTCATC TGGAAATTTT ATTGCAAACG ATGACTCATT TGGGAGCCTT 
CCATCGCCAA CAGAGCTTTC CACTTTCAAT TTCACCTCAA AGGCTAACCA AAAGAACTCA 
AAGTCTAGAA CAAAGAGCCT CCTTAAGAGG ATGGAAAGTC TGAAAATCAA AAGCTCTAAC 
AGTAAAAAGA AGTCACATTC CAAACTGGGT CTGATTATTA GTGAACCTAT CCTCCAAGAA 
GCGGTGGATG ATGATAAGCT AAAGCAACTT AACTGCGTAG AAATTTCCTC CCTTAATGGC 
AACCATATCA ACGCACCCAA GGTGCGGAAA AGGAGCACAT CAAACTCAAC TCAAACAAGT 
AGCAGTAGTA GCCAATCAGA CACTAGCAGT GCCGTAAGCA CTCCCAGTCC TGTAACAAGG 
ACAAGGAGTT TAAGTGCCTG CCATAAAAGA GTAGGTATGT ATTTAGAGGG CTTTGACCCT 
TTCAATCAGT CAACATTTAG TGATGTCCTC GAACAGAACT ATAAAAACCA GAGGACTTTT 
GGTGAGGATG CTGTGTTTTT TATTCCTGAA GACCACAAAC CTGGCACCTT TCCCAAGGCA 
CTTTCAAATG GCAATTTGTC TCCTTCTGAC AGTAGCCCCT CTGTCACCTG GAGAACTGGG 
AGTTTCCATG GACATGACAA CCTTAGCTTT AGAAGGGAAA ACAGCATCAG CAGCTGCAAA 
AGTTCGGTTA GCAGCCAGAG ACGGAATTCT TGTTGTTCTG AAGGAAGCCG CTTAAGTATT 
TATGACAATG TACCAGGAAC TCTTCTGTAT TCCAGTAACA GCGACCTAGC TGATCTGGAA 
AACGAAGACA TCTTTCCAGA ACTTGATGAT ATATTATACC ATGTGAAGGG GATGCAAAGG 
ATTGTGAATC AGTGGTCAGA GAAATTTTAT GATGAGGGAG ATTCTGATTC TGCTTTAGAT 
TCTGTATCTC CCTGCCCTTC CTCCCCCAAG CAGATACACT TGGATATTGA CAACGACTGC 
AATGCTCTTA GTGATCTCGA TAGCACAGGA AATTCATTTA ATGATTGTGA AGAAGCAACC 
GTAATCCAGG CGAGGAGAGA CTCCGGAGTT GGCGCTTCTC TGACGCGAAC TAACAGACAC 
CGGTTCAGAT GGCACAGCTT CCAGAGCTCC CACCGTCCCA GTTTAAGTTC TGCTTCATTG 
CAGATGAACT GCCAGTCCGT GACTCAGATC AACTTGCTTC AGAAGTATTC CCTCTTAAAG 
CTAACGGCTC TCCTGGAAAA ATATACCCCT TCCAACAAGC ACGGGTTCAG CTGGGCCGTG 
CCAAAATTTA TGAAACGCGT GAAAGTTCCA GACTACAAGG ACCGAAACGT GTTTGGCGTT 
CCTCTTGCAA TAAATGTACA GCGCACTGGT CAGCCATTGC CACAAAGCAT CCAGCTGGCC 
ATGCGTTACC TACGTAGTCA GTGCTTGGAT CAGGTGGGGC TTTTCAGAAA ATCAGGCGTC 
AAGTCCAGAA TCCTGGCTTT GCGGGAGATA AGTGAAAATA ACAACGACAG CTTGACCTAC 
GAGGGACAGT CTGCATATGA TGTGGCGGAC ATGTTAAAGC AGTATTTCAG AGACCTGCCA 
GAGCCACTTT TAACCAGCAA GCTTTCCGAA ACTTTCCTGC AAATCTACCA GTATGTCCCC 
AAGGAGCAGC GCCTGCAGGC AATCAAGGCG GCCATTATGC TGTTGCCTGA TGAAAACAGG 
GAAGTCCTGC AAACCCTTTT ATACTTCCTG AGTGATGTTG CAGCAGCTGT AGATGAGAAC 
CAGATGACCC CCACCAACCT CGCTGTCTGC TTGGCTCCTT CCTTGTTCCA CCTTAACACC 
TTGAAAAGAG AGAACTCCTC TCCCAGGGTT ATGCACAGAA AGCAGAGTCT AGGAAAGCCT 
GATCAGAAGG ACCTGAATGA AAACCTTGCT GCCACTCAGG GTTTGGCTCA CATGATTGCC 
GAGTGCAAGA AACTTTTCAA GATTCCTGAA GAAATGAGCA AGTGCCGTAA TTCATACATG 
GAGCACGACC TGAGACCCGT GTGCCTGGAG GAGCTTGTTA ATGGCAATGC AGAGGGGCTG 
TGTGACTATC AGTCGTACCT TCAAGAGAAC ATTGACGTCT TGCTCAAGGA TGCTAAAGAC 
AAATTCAAAG GCTGGGTCAA CTGTTCGACA TCAGAGCAAG CAGATCTCTC TTACAAAAAG 
GTGTGCGAAG GCCCTCCTCT TCGATTGTGG AAAGCCACCG TTGACATCCC TGCTCAGCCA 
GAAGATGTTC TTCACCGCTT GCTGAGAGAG CAGCACCTGT GGGATGAAGA TCTCCTGGAT 
TCCAAAGTTA TTGAGAGTTT GGATGACCAG ACTGATATTT ATCAATATGT GCAAAATTAC 
ATGGCGCCCC ATCCAGCCAG GGACTTTGTT GTATTAAGAA CATGGAGAAC CAACTTAGCG 
AAAGGCGCTT GTGTTCTTTC GTGCACTTCA GTGGATCACG ATCGTGCTCC TCAGCTCGGC 
GTCAGAGTAA ATGTTCTCAT GTCCAGATAC CTCATTGAAC CAGGCAGTTC GGGAAAATCC 
AAGCTCACAT ACCTGTGCAG AGCTGATTTG AGGGGCCATA TGCCAGAGTG GTACAATAAG 
GTTTTTGGGC ACGTGTGCGC AGCTGAAGTT GTAAGGATCA GAGACTCTTT TATGAACCAA 
CAATCAGAAA GCAAAGATGG AAAGTCCAGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031751275.1
CDS	471..3743
Translation	MCNEKLDRMILTQIEAKEACDWLRAAGFPQYAQLYEDLLFPIDISSVKKDHDFLDRDAIEALCRRLNTLNKCAVMKLEISPNRKRSEESDEDEPCAISGKWTFQRDSKRWSRLEEFEVFPLTQGPKALLSDAHQLGNAISQDSILTDLSEHQDVVSITSTSSESPRAHLPSDASTTRTNSVTSICSSGNFIANDDSFGSLPSPTELSTFNFTSKANQKNSKSRTKSLLKRMESLKIKSSNSKKKSHSKLGLIISEPILQEAVDDDKLKQLNCVEISSLNGNHINAPKVRKRSTSNSTQTSSSSSQSDTSSAVSTPSPVTRTRSLSACHKRVGMYLEGFDPFNQSTFSDVLEQNYKNQRTFGEDAVFFIPEDHKPGTFPKALSNGNLSPSDSSPSVTWRTGSFHGHDNLSFRRENSISSCKSSVSSQRRNSCCSEGSRLSIYDNVPGTLLYSSNSDLADLENEDIFPELDDILYHVKGMQRIVNQWSEKFYDEGDSDSALDSVSPCPSSPKQIHLDIDNDCNALSDLDSTGNSFNDCEEATVIQARRDSGVGASLTRTNRHRFRWHSFQSSHRPSLSSASLQMNCQSVTQINLLQKYSLLKLTALLEKYTPSNKHGFSWAVPKFMKRVKVPDYKDRNVFGVPLAINVQRTGQPLPQSIQLAMRYLRSQCLDQVGLFRKSGVKSRILALREISENNNDSLTYEGQSAYDVADMLKQYFRDLPEPLLTSKLSETFLQIYQYVPKEQRLQAIKAAIMLLPDENREVLQTLLYFLSDVAAAVDENQMTPTNLAVCLAPSLFHLNTLKRENSSPRVMHRKQSLGKPDQKDLNENLAATQGLAHMIAECKKLFKIPEEMSKCRNSYMEHDLRPVCLEELVNGNAEGLCDYQSYLQENIDVLLKDAKDKFKGWVNCSTSEQADLSYKKVCEGPPLRLWKATVDIPAQPEDVLHRLLREQHLWDEDLLDSKVIESLDDQTDIYQYVQNYMAPHPARDFVVLRTWRTNLAKGACVLSCTSVDHDRAPQLGVRVNVLMSRYLIEPGSSGKSKLTYLCRADLRGHMPEWYNKVFGHVCAAEVVRIRDSFMNQQSESKDGKSR

Target ORF information:

RefSeq Version	XM_031895415.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DLC1 Rho GTPase activating protein (dlc1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031895415.1

ORF Insert Sequence:

ATGTGCAACG AGAAGCTGGA CAGAATGATC CTCACACAAA TAGAGGCCAA GGAAGCATGT 
GACTGGCTAC GGGCAGCGGG ATTCCCACAG TATGCGCAGC TCTACGAAGA TCTTCTTTTT 
CCTATTGACA TCTCTTCCGT AAAGAAGGAC CATGACTTCT TGGACAGGGA TGCAATCGAG 
GCGCTCTGCA GGCGACTGAA TACTTTAAAC AAGTGTGCTG TGATGAAATT AGAAATTAGC 
CCAAATAGGA AAAGGAGTGA AGAGTCTGAT GAAGATGAAC CATGTGCAAT CAGTGGTAAA 
TGGACATTTC AAAGGGACAG CAAGAGGTGG TCTCGACTGG AAGAATTTGA AGTCTTTCCT 
CTTACTCAAG GACCAAAAGC TCTACTGTCA GACGCACACC AGCTGGGAAA TGCAATCAGC 
CAGGACAGTA TTCTTACTGA CCTTAGTGAG CACCAAGATG TAGTATCAAT TACCAGTACC 
AGCAGCGAAA GTCCCAGAGC ACACCTTCCC AGTGATGCCA GCACTACAAG AACAAATTCT 
GTCACCAGCA TTTGTTCATC TGGAAATTTT ATTGCAAACG ATGACTCATT TGGGAGCCTT 
CCATCGCCAA CAGAGCTTTC CACTTTCAAT TTCACCTCAA AGGCTAACCA AAAGAACTCA 
AAGTCTAGAA CAAAGAGCCT CCTTAAGAGG ATGGAAAGTC TGAAAATCAA AAGCTCTAAC 
AGTAAAAAGA AGTCACATTC CAAACTGGGT CTGATTATTA GTGAACCTAT CCTCCAAGAA 
GCGGTGGATG ATGATAAGCT AAAGCAACTT AACTGCGTAG AAATTTCCTC CCTTAATGGC 
AACCATATCA ACGCACCCAA GGTGCGGAAA AGGAGCACAT CAAACTCAAC TCAAACAAGT 
AGCAGTAGTA GCCAATCAGA CACTAGCAGT GCCGTAAGCA CTCCCAGTCC TGTAACAAGG 
ACAAGGAGTT TAAGTGCCTG CCATAAAAGA GTAGGTATGT ATTTAGAGGG CTTTGACCCT 
TTCAATCAGT CAACATTTAG TGATGTCCTC GAACAGAACT ATAAAAACCA GAGGACTTTT 
GGTGAGGATG CTGTGTTTTT TATTCCTGAA GACCACAAAC CTGGCACCTT TCCCAAGGCA 
CTTTCAAATG GCAATTTGTC TCCTTCTGAC AGTAGCCCCT CTGTCACCTG GAGAACTGGG 
AGTTTCCATG GACATGACAA CCTTAGCTTT AGAAGGGAAA ACAGCATCAG CAGCTGCAAA 
AGTTCGGTTA GCAGCCAGAG ACGGAATTCT TGTTGTTCTG AAGGAAGCCG CTTAAGTATT 
TATGACAATG TACCAGGAAC TCTTCTGTAT TCCAGTAACA GCGACCTAGC TGATCTGGAA 
AACGAAGACA TCTTTCCAGA ACTTGATGAT ATATTATACC ATGTGAAGGG GATGCAAAGG 
ATTGTGAATC AGTGGTCAGA GAAATTTTAT GATGAGGGAG ATTCTGATTC TGCTTTAGAT 
TCTGTATCTC CCTGCCCTTC CTCCCCCAAG CAGATACACT TGGATATTGA CAACGACTGC 
AATGCTCTTA GTGATCTCGA TAGCACAGGA AATTCATTTA ATGATTGTGA AGAAGCAACC 
GTAATCCAGG CGAGGAGAGA CTCCGGAGTT GGCGCTTCTC TGACGCGAAC TAACAGACAC 
CGGTTCAGAT GGCACAGCTT CCAGAGCTCC CACCGTCCCA GTTTAAGTTC TGCTTCATTG 
CAGATGAACT GCCAGTCCGT GACTCAGATC AACTTGCTTC AGAAGTATTC CCTCTTAAAG 
CTAACGGCTC TCCTGGAAAA ATATACCCCT TCCAACAAGC ACGGGTTCAG CTGGGCCGTG 
CCAAAATTTA TGAAACGCGT GAAAGTTCCA GACTACAAGG ACCGAAACGT GTTTGGCGTT 
CCTCTTGCAA TAAATGTACA GCGCACTGGT CAGCCATTGC CACAAAGCAT CCAGCTGGCC 
ATGCGTTACC TACGTAGTCA GTGCTTGGAT CAGGTGGGGC TTTTCAGAAA ATCAGGCGTC 
AAGTCCAGAA TCCTGGCTTT GCGGGAGATA AGTGAAAATA ACAACGACAG CTTGACCTAC 
GAGGGACAGT CTGCATATGA TGTGGCGGAC ATGTTAAAGC AGTATTTCAG AGACCTGCCA 
GAGCCACTTT TAACCAGCAA GCTTTCCGAA ACTTTCCTGC AAATCTACCA GTATGTCCCC 
AAGGAGCAGC GCCTGCAGGC AATCAAGGCG GCCATTATGC TGTTGCCTGA TGAAAACAGG 
GAAGTCCTGC AAACCCTTTT ATACTTCCTG AGTGATGTTG CAGCAGCTGT AGATGAGAAC 
CAGATGACCC CCACCAACCT CGCTGTCTGC TTGGCTCCTT CCTTGTTCCA CCTTAACACC 
TTGAAAAGAG AGAACTCCTC TCCCAGGGTT ATGCACAGAA AGCAGAGTCT AGGAAAGCCT 
GATCAGAAGG ACCTGAATGA AAACCTTGCT GCCACTCAGG GTTTGGCTCA CATGATTGCC 
GAGTGCAAGA AACTTTTCAA GATTCCTGAA GAAATGAGCA AGTGCCGTAA TTCATACATG 
GAGCACGACC TGAGACCCGT GTGCCTGGAG GAGCTTGTTA ATGGCAATGC AGAGGGGCTG 
TGTGACTATC AGTCGTACCT TCAAGAGAAC ATTGACGTCT TGCTCAAGGA TGCTAAAGAC 
AAATTCAAAG GCTGGGTCAA CTGTTCGACA TCAGAGCAAG CAGATCTCTC TTACAAAAAG 
GTGTGCGAAG GCCCTCCTCT TCGATTGTGG AAAGCCACCG TTGACATCCC TGCTCAGCCA 
GAAGATGTTC TTCACCGCTT GCTGAGAGAG CAGCACCTGT GGGATGAAGA TCTCCTGGAT 
TCCAAAGTTA TTGAGAGTTT GGATGACCAG ACTGATATTT ATCAATATGT GCAAAATTAC 
ATGGCGCCCC ATCCAGCCAG GGACTTTGTT GTATTAAGAA CATGGAGAAC CAACTTAGCG 
AAAGGCGCTT GTGTTCTTTC GTGCACTTCA GTGGATCACG ATCGTGCTCC TCAGCTCGGC 
GTCAGAGTAA ATGTTCTCAT GTCCAGATAC CTCATTGAAC CAGGCAGTTC GGGAAAATCC 
AAGCTCACAT ACCTGTGCAG AGCTGATTTG AGGGGCCATA TGCCAGAGTG GTACAATAAG 
GTTTTTGGGC ACGTGTGCGC AGCTGAAGTT GTAAGGATCA GAGACTCTTT TATGAACCAA 
CAATCAGAAA GCAAAGATGG AAAGTCCAGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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dlc1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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