speg cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	speg
Entrez Gene ID	100486621
Full Name	SPEG complex locus
General protein information	Preferred Names SPEG complex locus Names striated muscle preferentially expressed protein kinase
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031892844.1	XP_031748704.1	striated muscle preferentially expressed protein kinase isoform X2
XM_018097306.1	XP_017952795.1	striated muscle preferentially expressed protein kinase
XM_031892843.1	XP_031748703.1	striated muscle preferentially expressed protein kinase isoform X1
XM_031892847.1	XP_031748707.1	striated muscle preferentially expressed protein kinase isoform X4
XM_031892845.1	XP_031748705.1	striated muscle preferentially expressed protein kinase isoform X3
XM_031892848.1	XP_031748708.1	striated muscle preferentially expressed protein kinase isoform X5

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SPEG	NP_001135957.1
Mus musculus (house mouse)	Speg	NP_031489.4
Rattus norvegicus (Norway rat)	Speg	NP_001102272.1
Gallus gallus (chicken)	SPEG	XP_003641690.2
Danio rerio (zebrafish)	si:ch211-195m20.1	XP_691634.6
Homo sapiens (human)	SPEG	NP_005867.3
Pan troglodytes (chimpanzee)	SPEG	NP_001166952.1
Macaca mulatta (Rhesus monkey)	SPEG	XP_001104264.2
Canis lupus familiaris (dog)	SPEG	NP_001166949.1
Xenopus tropicalis (tropical clawed frog)	speg	XP_002934009.2

Gene Ontology

The following speg gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the speg cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa46745	XM_018097306.1 Latest version!	Xenopus tropicalis SPEG complex locus (speg), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa66222	XM_031892843.1 Latest version!	Xenopus tropicalis striated muscle enriched protein kinase (speg), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa66223	XM_031892844.1 Latest version!	Xenopus tropicalis striated muscle enriched protein kinase (speg), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa66224	XM_031892845.1 Latest version!	Xenopus tropicalis striated muscle enriched protein kinase (speg), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa66225	XM_031892847.1 Latest version!	Xenopus tropicalis striated muscle enriched protein kinase (speg), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa66226	XM_031892848.1 Latest version!	Xenopus tropicalis striated muscle enriched protein kinase (speg), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

speg ( XM_018097306.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017952795.1 Xenopus tropicalis SPEG complex locus (speg), mRNA.

Price & Availability↑

CloneID	OXa46745
Clone ID Related Accession (Same CDS sequence)	XM_018097306.1
Accession Version	XM_018097306.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	striated muscle preferentially expressed protein kinase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGCATCGTG CCTATGGGAG GAGGAACCTG GCCCGGAATG AAGAGGAAGC CCCTGTCTCC 
CCAACTCAAA CGTTTAGGTC TCCACCAAGC CCAGGCATTC CCCCAAAACA CCCCAAGTTG 
GCGGCTGAAC CTGGGCAGCC CTCTCCCCCT CAGTTTGTCC GGAAGCTGAA AAATGCTGCT 
ATAGGCACAG GCTGTGACAT CTGCCTAAAG GTGGCAGTGG CAGGGCACCC GCTGCCAACC 
CTAAACTGGT ACAAGAACCA GGAACGCATC CAACCCGGAG GAGAGGAATA TGGGACACTA 
TGCATCAGGG ACAGCAGGAT GGAAGATGCT GGAGTGTACA CCTGCGTGGC CCAGAATCCC 
CTGGGGGAGG CTATTACCTC TGCTGTGCTG GCAATCATTG AATTAGAAGA CTCAGAACCA 
GGGGAAGAAG ATGCAGGGGG CCCCCAATTA CAACGCACAA TTCGGGCACC CAACAATACG 
CCTCTGGGAA CCCCTTCAGG GGATTCAGAT ACGCTGCTGG CGAGCTCCCT GCACACCACG 
CCCAGCACGC TGACTGCACT CTCTCAAACA GACGAGCTCT CCGATTGGTC AGGCAGCCAA 
CAGACAGTAC TAGAAAGGGA CCCAAGGAAT GCAAGGCAAG GCTTCACTCA GGGAGGAGGG 
GCTCGCCATC TGGGGGTGGA GCCCCTCATT CCAGCTTCCA GCAACGAGTT CCCGGGCAGT 
GTCTGGGGGT CAGAGGTCAG CCTTTCTGGG TTCAGTGATC TGTATAGCAG CGCCTTCAGC 
CTGTACCGAG GCAGATCTTG TGCCATCCGG GTGTTCCTTC CGGAAGGGGG TCTGCGGAGG 
GGTAGCATCA ATGGTTCTGT AACAGACTCC CCACGAAACT TTGTCAATCC TAGGCCGCCT 
ATCATACCAC CACCCTCTCC GCGCATTGGC CAGCGTCCAG CTGTCATTCC TGCAGGCCGT 
GGACCCCCCA CCCCTGTTAC ACCTAGAAAG AAAACCACAA TGCCCAACCA GTACCAGGAC 
ACAGTGACTG AGGAGTTAGA AGAGAAGATT AAGAAGCCAA AGTCCTCTGT TGGCTCTCAG 
GCTAGTGGCC AAGATTCTCG CCCTGTAACT CCACTAAGTG AGACATCAGG GCGGGTGTCT 
ATCCTTAGGG CATCCCCAAA GCTAGTCCGC TCAGGCTCTA AGATCTTTGA AAAGCTTAGG 
TGTCTCGAAG AGAGGAGAAA AAGTCTGGAC CAGACAGACA GCCCCTTTCC AGTGCACTCA 
TGGTTGCCTC TCCGTAAGGC ACGATCCTTT GATCAGCCGG GGGCCGAGGG GCTCGGTGCT 
GGTCTAGGTT CCTCAAACGA AGAGCTAAGG GATGATCTTA GAGACGGGGT AAGATCAGAA 
ATAGGTGGGT TTACTCTGAG GTACCCCTCT CTGAGACACA AGACTACCTC ACTGGATGAC 
AGGGGGGCTC TCTATGGACG AATAAGCGAC ATTGAGACCC GCTTCTCACA AGAGCTGACT 
CGCATCAAAA GAACTGTGTC CCAGCAACAG CTAGTCCGTT CCTCACAGGA TCTATCTGGA 
AGATCACTGT CACCCCAAAG ATCCCTAGCT GCCCAGCCTG CTCCTGTGGA CCCTAAGCTA 
ACCAAAGTGT CAACGGCTCC AAAGCCTTCT GAAACAGAAG AGAAGCAGCC AGCAAGGAAG 
CCACTAGTCA GAAGCTTTGC CACCGTCAAC CAACTGTCAG AAATTAAGGA ACCTCTGGGG 
TTTTCAAGCC AACCCCCTAC ACTGCCATCA AAATCATCCA ATGGTTCAGC AGTATATCAG 
GATGCAGTGA GGCACTCGGA AACAAATATG AAATCACAAC CACACCTTAC AGGAAAAATC 
ATTGCACCAA AAACTGTTTC CCCACAAGTA TTTAAAGCTA TGACTCTCCC TGCACAATCC 
CAAAAAGAGG CAGTCGGCGA CACACAGCCA AAGAGAGTTC CTGATGTTGG TAGCGTGCCT 
CCACCACCCA GCACACATGC CCCGGCAGCC CTAGAGATAG CTCAGAGGGG ACTTGTTAAA 
GAGAAGGGGA CTGTAAGCGA AGAGAAGAAG TATCTACCCT GGGCACTTCC TGTAGGAGAC 
AATAACAACA GCCAAATGCC ACAGGGTAAG GGTGGGGGAC AGGCCAAAAA GCACAGCGAT 
GCACACACCA GCAGCAAAAG CAACAAAAGC GGCAGAAGCA AAGGGAAGAG TCGCCGAAGC 
AGGCCATTGT CACCAGAACT TGAGTCATCG GATGATTCCT ACGTATCGGC GGGAGAGGAT 
CCAGCAGAGG CGCCTGTGTT TGAGATTCCT TTGCAAAGTG CACTAGTAAA CGCCGGCACT 
GAAGTCTTAC TGAAATGTAT TATATCTGGA AACCCAAGTC CAGAAGTTTT TTGGAGAAAG 
GATCACGTGT TGCTGAAAAA CAGTCCGACT CATCAAATAC GAGTAGAGGG CGAGAGACAC 
ACGTTGCTAC TGCGCTGGGC ACTGCCCTCA GACTCGGGTA TCTATACAGT GACTGCGCGG 
AATGAAGTTG GGGAAGCCAG CAGCTGCGGT GCCCTCACTG TAAAACCAGC ACCTACCAAA 
GAGTCGCCGG CACATCGGGG AACACCTCGT GACCTGCTCA GCCCAATAAC ATCGGATGAA 
GAGTATCTAA GCCCCCAGGA AGATTTAAGT GAGCCTACCA CACCCCAACA TAAGATGGGA 
GGCAAAGGCC CCGCATTCAA AGCTCCACCC ACTTTCAAGA TGCCTCTTTT GGACCAGAAA 
GGGTTTGAAG GCCAAGAGAT TGTTCTCAGC GTTCAGGTGG AAGGGGAACC CAAGCCTATT 
ATCAACTGGC TGAAGAACAA GCAGCAGGTG AAGCCTGGTG GAAGGTTCCG CATATCAGAA 
GGTGCTTGGG GGATCTTCTC GCTACATATT GCCGGGGCTG ATAAACGAGA TTCCGGATTC 
TACACCTGTA AAGCAATAAA TGAGTATGGG ACAAAGCAAT GTGAAGCCAA GATAGAGGTT 
CTAGCACCCC CTGGCAGTAC CAGCTTGGAG GTTCTGGCCC CCTTGCAGGA TGTGAGTGTG 
TGCGCTGGGG AGGCAGCGGT GTTTGAATGC GTGGTGTCGG GCCCCGCCGA TATGGACGTG 
GACTGGATGT TCCGGGGCAA GCTGCTCCAG CCAGCACTGC TGGACTGTAA GATGCGTTTC 
AACGGAAAGC AGTGTGTGCT GCTGCTGAAT TCAGTCCATG AAGATGACAG TGGGGTCTAC 
ACCTGCAAAC TGAGCACTGC AAGAGATGAG CTGACCTGTA GCGCTATGCT AACTGTGCAC 
CCATCGCTGG CGCCTCTCTT CACTCGCAAG TTGTCAGATC GAGATGTTAT AGAGGGACGC 
ACAGCTCGGC TGGACTGTAA AATCAGTGGC ACCCCGCCCC CTGTTGTCAC GTGGACTCAT 
TTTGGACAGC CCATAGAGGA AAGTGAGAAC ATCCACGTTT TGAGAGAAGG CGGACATCAC 
ACCTTACTGA TACCCCACGT GGCGAATGAG GACGAGGGTC AGTACACAGC GACTGCCCAT 
AACAAGCATG GGGAGGCCGA ATGCTCTGCT GAACTCTACG TGGAGGAGCC TCGTTCTGCT 
ACAGCATCTC ATATCTCCAA GCTGGAGAAG ATGCCATCTA TTCCAGAAGA GCCAGAGATG 
CAGGAGTGTG AGGTAGAGGG TGTGATGATG CCGGATTTCC TGCGACCTCT GCAGGACATT 
GACGTGGTGG AATTTCGGGA GGTACAACTT GAGTGTCAGG TAACCGGTCT GCCGTACCCT 
ACCATCACCT GGTATCACAA TGGTCAGAAG ATCCAGAGCT CTGATGATCG CAGAATGACT 
CAATATAAAG ACATCCACCG CCTGGTTTTC CCCTCTGTTA CTCATTCTCA TGCCGGGGTC 
TACAAAAGTG TCATCTCAAA CAAAGTGGGG AAAGCAGCCT GCTATGCGCA CCTGTATGTC 
ACAGATTTGG TACCCACTCC TCCAGATGGT CCCCCCATCA TTACCTCTGT AACTGGGAGA 
ATTGTGAAGC TCCAGTGGAA GCCACCCAAG AGATTGGACC CAGCCATTGA TCTGGCTCAG 
ATTACATACT CAGTCCAGCA ACAAGCGGTG GGATCTCCCC AATGGACAGT CATCGCCACC 
AACCTAAAAG ACACTTGTTA TTGTGTCAGA TCTCTGACCA AGGAACATCA GTATCTTTTC 
CGTGTGACCA CAAACACCCC AACTTCACAC AGTAAACCTT CCCCTCCCAG TGAAAGCGTT 
TGTCTCTTTA ATAGAGGTCC CTACCTGGAG GAGATGCCTT CTATACTGGA TCGGCCTGAA 
ATCTTGTATA TGGTGGAAAA TCAGCCTCTT TGCATAACCG TGACGCTGAA CCACGTACAT 
GCCAAAGTCA CGTGGCAGAG AATAGACACC GTTCTGAGCA ATGTCGAGGG TGTGTATGAG 
CTGAGTATGC CGGATGACGA CCAGCACTCG CTCACGTTCT TCAGCCCAAG GAAGTCTGAC 
CTGGGACAGC TGACATTTGA AGCTAAAAAC CGCTACGGAT CAGATAGCTG CACTATTTCC 
ATCGAGCTCG CAGAGGCTCC TCGCTTTGAG TCCATCATGG AAGACATAGA GATCGGTGTT 
GGTGAAACAG CTCGTTTTGT GGTGGTGGTG GAAGGGAAGC CTATTCCAGA CATCATGTGG 
TACAAAGATG GGGTACTTTT GGCAGAGAGC GGTCACTTCA GCTTTGTGTA TGATGATACC 
GAGTGCTCTC TGGTGATCTT AAACACGGCA CTTGGTGACT CCGGTGTGTA CACCTGCACG 
GCACGGAACC TTTCAGGGGA AATTTCCTGC AAGGCGGAGC TGCAAGTGTG CCCAGATGAA 
CCAGGGGCAG GATCTGCAAT ACAGGAAATA GATATACTCA AAGTTCGTAG ATTGACTGAT 
TTCTACAACA TCCATAAAGA AATTGGCAGA GGGGCCTTCT CCTATGTCCG ACATGTGGTA 
GAAAAGAGCA ACGGGCGAGA TCTTGCTGCT AAATTCATAC CGTTCCGTGG GGCAACCAGG 
GAGAGTGCAC GGAGGGAGCG GGACATACTG TCCAAGCTCA GACACGACAG GATCATCTTC 
TTCTACGATG CATTCGAGAA AAGAAGTGCT CTAATCATTG TCATGGAACT CTGTTCTCAA 
GAGGAGCTTC TAGAGAGGTT GACAAGAAAG CCTACAGTGA GCGAATCAGA GATCCGCTCG 
TTTATACGGC AAATCCTAGA AGGGTTAGAT TATTTGCACC ATAAAAACAT TCTGCATTTG 
GACATAAAGC CAGAGAACAT CCTGATGGCC GATACGACTA GTGAACAGAT TCGCATTTGT 
GACTTTGGAA ACGCTCAGGA ACTAACCCCG GGGGATCCTC AGTACTGCAA ATACGGAACC 
CCGGAATTTG TGGCGCCCGA AATTGTGAAC CAGATGCCAA TTTCTACAGT AACGGACATA 
TGGCCAGTTG GAGTCCTGGC ATATTTATGC CTTACCGGAG TGTCGCCCTT TGTTGGGGAG 
AATGATTACA CCACTCTGAT GAATATCCGG GGTTACACTG TGGCATTTGA AGAGAAGATG 
TTTGTTGGTC TCACACGAGA AGCACGTGGT TTCCTAATCA AAGTCTTGGG CAATGAGAAG 
CTGAGGCCTA ATGCTGAAGA GAGTTTGGAG CACCCCTGGT TCAAAACTTT AGCGAAAGGG 
AAAAGCATCA GCACAGACCA TCTGAAGCTG TTCCAGTCTC GCCGTAAATG GCAGCGCTCA 
CTTATCAGCT ATAAGTCCAA TATGGTGATG AGAACCATTC CAGAGCTGCT TCAGGACACA 
AGCAATCACT TGTCCATTGC TGTACCCAAG AATTCCAAGG AAGCATCCGG ATTATCATCC 
TCCTCTGATT CTGATGACCT GGAAGAGCTT CCCTTTGTCC CTATGCCTCT CCAGGTCCAA 
TTCTCTGGAT CACGCATGTC CTTGAATGAG ATCCCAACAG ATGAGGAACA ACTGTCTCAG 
CCCCAGGACA ATGGCAGCTG TAGAGAAGAT GAGGAAGAAG GAAGTCAGGC AGTGGAAATT 
AGTAGCACAT TTAAAGAACA AGCAGTTCAG CAGAAAGAAG CAACAGTCCA AGCAAGTCCA 
TTGTCCAAGC ATGCCAAAGG AACCCTTACA AGGAACAGGT CTGCTGAAGT TGGTTCTTCT 
TCTGATGAGG AAACTTCAGA AACTGAAAAA AGAGAGCATC CTCGTAGGCC TCTGAAAAAA 
GGTTCCAGCT TAGAATCTGC CGACGTTCCT GATGGGGAAA TTAAAGCAGC TCGTAGAGGA 
GAGCTGAGGA GGGGAAGCTC TGCAGATAGT GCTCTTTTGC TCAATGTGTC TACAACAGAT 
GGAGATGAAG AGAGAGATCG ACCAGACAAG GGAATGATAA AAGCTGCCTC AATGGAGCTA 
CCAACCAGAT GCAGGAGCCC AGGTAGATTG GAGGATCGAG GCACACTACG AAGAAAGTTT 
GGATCAGCTG ATGAAGAATA TGCTCAGCGG TTAGAAATGA TGAGACAGAG GCTATTGAGG 
GGAGGTTCGG CAGATGGAAA ACTCAGTGGT CTTAGAGGCC CACTCATAGA AACACTTTCT 
GTAGACAAGA AGCGAGCAGA GCAGATATCT ATACGCTCCC CAAGAAGTGA TCAGTCTTCT 
CTGAGTCCTA TTCCCGCCAT CAAACTAACC CGAGCTGCTT CCAGTGAGGC TGCGCCTGGT 
AGAGAAGCAA CCGAAGAAAG AGTCTTAAGG AAAACCAGTT CCTTTTCTCA CAGCGACACA 
GAGCCACTGG TCTTGCATCG CAGGTCAGGG GCACCCCTAG AAATACCTCT TGCTCAGTTA 
GAGGCACAGC GTCTTAAAGA GTCTCCCTCT CTTTCAGCAT TGACAGACCG ATTAGAATCA 
CGTCCTCAAA CACCGCGAGA GATGATTCCC AAATCTCTAA CTCCAGAAGC ACCGCAATTG 
AAGGCAGAAA GTGGGGAGCC AGAAACTCAG AGTGATAAGG AAGCTGAGAA GGGCACCACA 
CCTCTTATTC AGCATCATAA AGACAAAGAC TCAAATTCAT TGAAGGACTT GGTTATTGTT 
CTTCCAAGAG TGACTCCACC TTTGGATAAA ATGGCATCTA AAACAGAGTT ACCAAGTATT 
CAAACAGAGA GGGCACAGGA CAAAATCCCT AGAGCAGATG TGAACCTGCC AGCTACTACT 
TCATCAAATG AAACTGTGCC AACAAAGCCA CCACCCTTAC TTTTGCCTTC TAAGCAGTCT 
CTTTCTGTGC CACCTGAACC TTTACCCTCT TCAGTACTGA CAAATGAGAT GCCTAAATCT 
AAAGAGGAGT ACTCGTCATC AAGGGCCATT AAGGCCAAGG AATCCATATC AAAAACAGAA 
ACAACAGACC AGCAAGATAA AATTAAAGAA CCTTTACCAC AAGCTGTTGT TCCATTACCA 
TTGCCTATGC CAGCCTCCCA AGTGCTAAAA CCTTCCACTG CAAAAGTACA GATAGCAGAT 
TCTCCAACAA TGCCTGAGAA TGATGCATCA CTTCCACAGA TAACAGGTAA AGAGAAACCA 
TCTTCTCCAG CTGTCCAACC AGTAGATAAA ACAGCAGCTA TATCATCACC TTATGCTGCT 
ATGATTCAAA CACTTCAAGT GCCCGAGGAA CCTGAGACAG TTGTTGAGGA ATGTTCAAAG 
GCCCCATTAA AAGCTCAGCC AGTATTTCAA GAAATTCCTA TAGCCACTGG AGATGCAAAG 
GCTCAAGGAA TATCACAAGT AAACAATAAA GAAGGCAAGG ATGAGCTATC ACAAGAACCC 
AAGCTGATTA ACACCAGATC TTCCAGCACA GAGAATGTCC CATTTATCAG CAACATTGAT 
TCAGAAGAAA TCTTTGAGGC TAAGTTCAAG AGGAACCGTG AAAATTCTCT TACCCGAGGT 
CTAAAAAAGC TTACCAGGAC TTGGTCAGAG GAGAAGAATT TAGCTGCAGC CCATGCATCT 
CGGGAAGAAG AAATGTACAG GCCTAGTCCT GTGGGTGTCC CACTAGAGTT CCTTGTTCCT 
GCAGCTCTTG GGCTACATGA AAGATCAAGA TCAGTGCAAG ATCTGCCAGA TGCTGAGAGG 
GATTCAAGCT TTATGAGAAA GCTTTCTTTG AGATTTAAGA AAACTCCACC AACTGAAAGG 
AAGCAGAAAC CAGCAGAAGA AGTAGAAGGT ACTGGTAGAC GACTTTCATG GTCAATAGGT 
AGAGGAAGTT CCAAAGACAA GAAGGATACA GAGAGCCTGA ACTCTGAAAC AGGCTCAATA 
GAAACCATCA CAGACAAACA TCTAAAGGAC CAGAAAAAGA GTAGTCAATC ACCAGTGTTG 
GCCATGAGAA AGAAAATAGG CAGTACTATG GATCGACTTT CTATGAAACT AAGGAGCCAA 
TCAGAAGATA GAAGGGATGC AGAGAGAACA GAGAAGAAAG AAGGGCGTAC AGACAAAAGC 
TCTCCATTAA TGTCTTTACT TAGGAGATCC AACTCAGAAG GAGAGAACTT AAAGAAACTA 
GGTATTCCCC AGAACCAGCT TGCTTCCCAG GTTTCAGCTC CATCAACTGA GTCTATTGAA 
TCTGGTCTCA GCATCAAATC TGAAATGGTA GTCAAGGATG ATAGGCGCTC AAGGTGGGAT 
CGTTGGGGCC TGTCACGTAG CAAAAAGGAG AAGATGACAT CGCAGCCAAG TATTCCAGCC 
AGTCTGATGC ATGAAGATGG CAGTATTGTT GGCAGACAAT ACATTCGTAA TGAGTCAGAC 
TTCCCCCCAG TCTTCCATAT CAAGCTGAAG GACATCGTGG TGCTAGAGGG AGAGCCGGTG 
ACTCTGTCCT GCTTACCTGC TGGCAGCCCT TCCCCCCAGA TCCTGTGGAA GAAAGATAAG 
ATGTCCTTGG AAAGAGGAGA AAAGGTGCAT ATAAAATCCT GCCCCGATGG CAGACAGCAG 
CTCATTATTC CCGCCGCCGG GCAGAGGGAA GCCGGTCTGT ATGAATGCGT AGCAGCCAAT 
GCCCTGGGCT CAGTGGCCAG CTCCTGCACA GTGTCTGTGG CAAGAATACC TGATCCCCCG 
GGGACCCCAG AGATCCCACA GAAGTATAAG AACACGGTGC TGGTTCTATG GAAGACCCAC 
GAGCACAACT CCCCTTGCAC CTACATCCTG GAGAAACAGG TTAATGGTGA TGGGGAATGG 
AAGGTTCTAA GTTCTGGAAT CACCGACTGT TACTTCAATG TGACGGAGCT CCAGGAAGGC 
ACTGTCAGGT TCCGCGTCGC TTGTGCCAAC AAAGCAGGAA AGGGGCCCTA CAGCAATGTG 
TCCAAGACAA TCGTCGTAGA TGCAGAAGAT CGTAAACCAG GAACACCCTC TGTCCAGAAG 
GCACGGCCCG TGGGAGCTGC AGTGTCCACT ACTGTTCCAA TGACTCTCCG GACCCCTTCT 
ACCCCAGCTG TTGCGCCCCC TGTTGTCTCT ACCCCCAAAT TACCTCCATC TTGTGGATCT 
GCTCTTGCAG TTTCCTCAAC AGTTATCACT ACAACCGCAA CTGTGTCTCC AGCCCAGCCT 
CCCACCCCTC CTACCCCCAT TAGCCCCACT TCCCCAACCG CCCCATTTAC CCCCACTTCC 
CAAACTGTCC CTACACGGGT GATAGTAAGA AGTGTCACCC CCGGGAAGGA TGCACGTTAT 
ACACCCGGAG GAAGAGTCAC ACCCTCGGGG AGAGAAAGCA CCACCCTCCG ACAAGGGGTA 
CCCCAAAAAC CTTACACTTT CTTGGATGAG AAAGCAAGGG GGCGGTTTGG AGTGATCCGA 
GAGTGTAAGG AGAATGCAAC TGGGAAGCAC TTCATGGCAA AGATAATCCA GTACGAACCA 
GAAAACAAGC AAAACGTGCT GCAGGAGTAT GAGATCCTCA AGGGTCTCCA CCATCAACGA 
ATCCTGAGTC TTCATGAAGC TTATATTACC CCTCGCTACC TTGTGTTGAT TTCTGAAAAC 
TGCACAGGAC GGGAACTGCT TTACTGCCTA GTTGAGAGGT TCCGTTATTC TGAAGACGAC 
GTGGTCAATT ACCTCCTGCA GATCCTACAG GGGTTGGAGT ACCTCCATGA ACAAAAGGTC 
CTGCACCTCG ATATCAAACC TGAGAACGTC ATTGTGTCCT ACATGAACAC TGTCAAGATC 
ATCGATTTGG GGAGTGCCCA GAACTTTACA CCGCTGGTCC TGCGACCCCT CGGCAAACGC 
ATAGGGACCC TGGAATATAT GTCCCCCGAA ATGCTCAAAG GAGACGCAGT AGGCCCAGCA 
GCTGATATTT GGGGTGTAGG TGTTCTGACG TACATAATGC TCAGCGGCCG CTCTCCGTTC 
TTTGAGCTGG ACCCCGTGGA AACCGAGCAC AAGATCTTGT CGGGACGATT TGATATCTTT 
AAATTGTATT CCAACGCCTC CCAAAGTGCT TCCCTCTTCA TTCGCAAGAT CCTGACTATA 
TATCCTTGGA GCCGCCCATC ACTTAAGGAA TGTTTTTCCA ATCCCTGGCT CCAGGATGCC 
TACCTAATGA AGCTTAGACG CCAAACCCTA ACCTTCACCA CTAACAAGCT CAAAGAGTTC 
CTAGTGGAGC AACAGAGGAG ACGCGCAGAC ACAGCCACTA AGCACAAAGT CCTCCTTCGT 
TCGTACCACG GAAGCCAGCC CTCAGCTCCT GTCACTCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017952795.1
CDS	448..11109
Translation	MHRAYGRRNLARNEEEAPVSPTQTFRSPPSPGIPPKHPKLAAEPGQPSPPQFVRKLKNAAIGTGCDICLKVAVAGHPLPTLNWYKNQERIQPGGEEYGTLCIRDSRMEDAGVYTCVAQNPLGEAITSAVLAIIELEDSEPGEEDAGGPQLQRTIRAPNNTPLGTPSGDSDTLLASSLHTTPSTLTALSQTDELSDWSGSQQTVLERDPRNARQGFTQGGGARHLGVEPLIPASSNEFPGSVWGSEVSLSGFSDLYSSAFSLYRGRSCAIRVFLPEGGLRRGSINGSVTDSPRNFVNPRPPIIPPPSPRIGQRPAVIPAGRGPPTPVTPRKKTTMPNQYQDTVTEELEEKIKKPKSSVGSQASGQDSRPVTPLSETSGRVSILRASPKLVRSGSKIFEKLRCLEERRKSLDQTDSPFPVHSWLPLRKARSFDQPGAEGLGAGLGSSNEELRDDLRDGVRSEIGGFTLRYPSLRHKTTSLDDRGALYGRISDIETRFSQELTRIKRTVSQQQLVRSSQDLSGRSLSPQRSLAAQPAPVDPKLTKVSTAPKPSETEEKQPARKPLVRSFATVNQLSEIKEPLGFSSQPPTLPSKSSNGSAVYQDAVRHSETNMKSQPHLTGKIIAPKTVSPQVFKAMTLPAQSQKEAVGDTQPKRVPDVGSVPPPPSTHAPAALEIAQRGLVKEKGTVSEEKKYLPWALPVGDNNNSQMPQGKGGGQAKKHSDAHTSSKSNKSGRSKGKSRRSRPLSPELESSDDSYVSAGEDPAEAPVFEIPLQSALVNAGTEVLLKCIISGNPSPEVFWRKDHVLLKNSPTHQIRVEGERHTLLLRWALPSDSGIYTVTARNEVGEASSCGALTVKPAPTKESPAHRGTPRDLLSPITSDEEYLSPQEDLSEPTTPQHKMGGKGPAFKAPPTFKMPLLDQKGFEGQEIVLSVQVEGEPKPIINWLKNKQQVKPGGRFRISEGAWGIFSLHIAGADKRDSGFYTCKAINEYGTKQCEAKIEVLAPPGSTSLEVLAPLQDVSVCAGEAAVFECVVSGPADMDVDWMFRGKLLQPALLDCKMRFNGKQCVLLLNSVHEDDSGVYTCKLSTARDELTCSAMLTVHPSLAPLFTRKLSDRDVIEGRTARLDCKISGTPPPVVTWTHFGQPIEESENIHVLREGGHHTLLIPHVANEDEGQYTATAHNKHGEAECSAELYVEEPRSATASHISKLEKMPSIPEEPEMQECEVEGVMMPDFLRPLQDIDVVEFREVQLECQVTGLPYPTITWYHNGQKIQSSDDRRMTQYKDIHRLVFPSVTHSHAGVYKSVISNKVGKAACYAHLYVTDLVPTPPDGPPIITSVTGRIVKLQWKPPKRLDPAIDLAQITYSVQQQAVGSPQWTVIATNLKDTCYCVRSLTKEHQYLFRVTTNTPTSHSKPSPPSESVCLFNRGPYLEEMPSILDRPEILYMVENQPLCITVTLNHVHAKVTWQRIDTVLSNVEGVYELSMPDDDQHSLTFFSPRKSDLGQLTFEAKNRYGSDSCTISIELAEAPRFESIMEDIEIGVGETARFVVVVEGKPIPDIMWYKDGVLLAESGHFSFVYDDTECSLVILNTALGDSGVYTCTARNLSGEISCKAELQVCPDEPGAGSAIQEIDILKVRRLTDFYNIHKEIGRGAFSYVRHVVEKSNGRDLAAKFIPFRGATRESARRERDILSKLRHDRIIFFYDAFEKRSALIIVMELCSQEELLERLTRKPTVSESEIRSFIRQILEGLDYLHHKNILHLDIKPENILMADTTSEQIRICDFGNAQELTPGDPQYCKYGTPEFVAPEIVNQMPISTVTDIWPVGVLAYLCLTGVSPFVGENDYTTLMNIRGYTVAFEEKMFVGLTREARGFLIKVLGNEKLRPNAEESLEHPWFKTLAKGKSISTDHLKLFQSRRKWQRSLISYKSNMVMRTIPELLQDTSNHLSIAVPKNSKEASGLSSSSDSDDLEELPFVPMPLQVQFSGSRMSLNEIPTDEEQLSQPQDNGSCREDEEEGSQAVEISSTFKEQAVQQKEATVQASPLSKHAKGTLTRNRSAEVGSSSDEETSETEKREHPRRPLKKGSSLESADVPDGEIKAARRGELRRGSSADSALLLNVSTTDGDEERDRPDKGMIKAASMELPTRCRSPGRLEDRGTLRRKFGSADEEYAQRLEMMRQRLLRGGSADGKLSGLRGPLIETLSVDKKRAEQISIRSPRSDQSSLSPIPAIKLTRAASSEAAPGREATEERVLRKTSSFSHSDTEPLVLHRRSGAPLEIPLAQLEAQRLKESPSLSALTDRLESRPQTPREMIPKSLTPEAPQLKAESGEPETQSDKEAEKGTTPLIQHHKDKDSNSLKDLVIVLPRVTPPLDKMASKTELPSIQTERAQDKIPRADVNLPATTSSNETVPTKPPPLLLPSKQSLSVPPEPLPSSVLTNEMPKSKEEYSSSRAIKAKESISKTETTDQQDKIKEPLPQAVVPLPLPMPASQVLKPSTAKVQIADSPTMPENDASLPQITGKEKPSSPAVQPVDKTAAISSPYAAMIQTLQVPEEPETVVEECSKAPLKAQPVFQEIPIATGDAKAQGISQVNNKEGKDELSQEPKLINTRSSSTENVPFISNIDSEEIFEAKFKRNRENSLTRGLKKLTRTWSEEKNLAAAHASREEEMYRPSPVGVPLEFLVPAALGLHERSRSVQDLPDAERDSSFMRKLSLRFKKTPPTERKQKPAEEVEGTGRRLSWSIGRGSSKDKKDTESLNSETGSIETITDKHLKDQKKSSQSPVLAMRKKIGSTMDRLSMKLRSQSEDRRDAERTEKKEGRTDKSSPLMSLLRRSNSEGENLKKLGIPQNQLASQVSAPSTESIESGLSIKSEMVVKDDRRSRWDRWGLSRSKKEKMTSQPSIPASLMHEDGSIVGRQYIRNESDFPPVFHIKLKDIVVLEGEPVTLSCLPAGSPSPQILWKKDKMSLERGEKVHIKSCPDGRQQLIIPAAGQREAGLYECVAANALGSVASSCTVSVARIPDPPGTPEIPQKYKNTVLVLWKTHEHNSPCTYILEKQVNGDGEWKVLSSGITDCYFNVTELQEGTVRFRVACANKAGKGPYSNVSKTIVVDAEDRKPGTPSVQKARPVGAAVSTTVPMTLRTPSTPAVAPPVVSTPKLPPSCGSALAVSSTVITTTATVSPAQPPTPPTPISPTSPTAPFTPTSQTVPTRVIVRSVTPGKDARYTPGGRVTPSGRESTTLRQGVPQKPYTFLDEKARGRFGVIRECKENATGKHFMAKIIQYEPENKQNVLQEYEILKGLHHQRILSLHEAYITPRYLVLISENCTGRELLYCLVERFRYSEDDVVNYLLQILQGLEYLHEQKVLHLDIKPENVIVSYMNTVKIIDLGSAQNFTPLVLRPLGKRIGTLEYMSPEMLKGDAVGPAADIWGVGVLTYIMLSGRSPFFELDPVETEHKILSGRFDIFKLYSNASQSASLFIRKILTIYPWSRPSLKECFSNPWLQDAYLMKLRRQTLTFTTNKLKEFLVEQQRRRADTATKHKVLLRSYHGSQPSAPVTQ

Target ORF information:

RefSeq Version	XM_018097306.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis SPEG complex locus (speg), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018097306.1

ORF Insert Sequence:

ATGCATCGTG CCTATGGGAG GAGGAACCTG GCCCGGAATG AAGAGGAAGC CCCTGTCTCC 
CCAACTCAAA CGTTTAGGTC TCCACCAAGC CCAGGCATTC CCCCAAAACA CCCCAAGTTG 
GCGGCTGAAC CTGGGCAGCC CTCTCCCCCT CAGTTTGTCC GGAAGCTGAA AAATGCTGCT 
ATAGGCACAG GCTGTGACAT CTGCCTAAAG GTGGCAGTGG CAGGGCACCC GCTGCCAACC 
CTAAACTGGT ACAAGAACCA GGAACGCATC CAACCCGGAG GAGAGGAATA TGGGACACTA 
TGCATCAGGG ACAGCAGGAT GGAAGATGCT GGAGTGTACA CCTGCGTGGC CCAGAATCCC 
CTGGGGGAGG CTATTACCTC TGCTGTGCTG GCAATCATTG AATTAGAAGA CTCAGAACCA 
GGGGAAGAAG ATGCAGGGGG CCCCCAATTA CAACGCACAA TTCGGGCACC CAACAATACG 
CCTCTGGGAA CCCCTTCAGG GGATTCAGAT ACGCTGCTGG CGAGCTCCCT GCACACCACG 
CCCAGCACGC TGACTGCACT CTCTCAAACA GACGAGCTCT CCGATTGGTC AGGCAGCCAA 
CAGACAGTAC TAGAAAGGGA CCCAAGGAAT GCAAGGCAAG GCTTCACTCA GGGAGGAGGG 
GCTCGCCATC TGGGGGTGGA GCCCCTCATT CCAGCTTCCA GCAACGAGTT CCCGGGCAGT 
GTCTGGGGGT CAGAGGTCAG CCTTTCTGGG TTCAGTGATC TGTATAGCAG CGCCTTCAGC 
CTGTACCGAG GCAGATCTTG TGCCATCCGG GTGTTCCTTC CGGAAGGGGG TCTGCGGAGG 
GGTAGCATCA ATGGTTCTGT AACAGACTCC CCACGAAACT TTGTCAATCC TAGGCCGCCT 
ATCATACCAC CACCCTCTCC GCGCATTGGC CAGCGTCCAG CTGTCATTCC TGCAGGCCGT 
GGACCCCCCA CCCCTGTTAC ACCTAGAAAG AAAACCACAA TGCCCAACCA GTACCAGGAC 
ACAGTGACTG AGGAGTTAGA AGAGAAGATT AAGAAGCCAA AGTCCTCTGT TGGCTCTCAG 
GCTAGTGGCC AAGATTCTCG CCCTGTAACT CCACTAAGTG AGACATCAGG GCGGGTGTCT 
ATCCTTAGGG CATCCCCAAA GCTAGTCCGC TCAGGCTCTA AGATCTTTGA AAAGCTTAGG 
TGTCTCGAAG AGAGGAGAAA AAGTCTGGAC CAGACAGACA GCCCCTTTCC AGTGCACTCA 
TGGTTGCCTC TCCGTAAGGC ACGATCCTTT GATCAGCCGG GGGCCGAGGG GCTCGGTGCT 
GGTCTAGGTT CCTCAAACGA AGAGCTAAGG GATGATCTTA GAGACGGGGT AAGATCAGAA 
ATAGGTGGGT TTACTCTGAG GTACCCCTCT CTGAGACACA AGACTACCTC ACTGGATGAC 
AGGGGGGCTC TCTATGGACG AATAAGCGAC ATTGAGACCC GCTTCTCACA AGAGCTGACT 
CGCATCAAAA GAACTGTGTC CCAGCAACAG CTAGTCCGTT CCTCACAGGA TCTATCTGGA 
AGATCACTGT CACCCCAAAG ATCCCTAGCT GCCCAGCCTG CTCCTGTGGA CCCTAAGCTA 
ACCAAAGTGT CAACGGCTCC AAAGCCTTCT GAAACAGAAG AGAAGCAGCC AGCAAGGAAG 
CCACTAGTCA GAAGCTTTGC CACCGTCAAC CAACTGTCAG AAATTAAGGA ACCTCTGGGG 
TTTTCAAGCC AACCCCCTAC ACTGCCATCA AAATCATCCA ATGGTTCAGC AGTATATCAG 
GATGCAGTGA GGCACTCGGA AACAAATATG AAATCACAAC CACACCTTAC AGGAAAAATC 
ATTGCACCAA AAACTGTTTC CCCACAAGTA TTTAAAGCTA TGACTCTCCC TGCACAATCC 
CAAAAAGAGG CAGTCGGCGA CACACAGCCA AAGAGAGTTC CTGATGTTGG TAGCGTGCCT 
CCACCACCCA GCACACATGC CCCGGCAGCC CTAGAGATAG CTCAGAGGGG ACTTGTTAAA 
GAGAAGGGGA CTGTAAGCGA AGAGAAGAAG TATCTACCCT GGGCACTTCC TGTAGGAGAC 
AATAACAACA GCCAAATGCC ACAGGGTAAG GGTGGGGGAC AGGCCAAAAA GCACAGCGAT 
GCACACACCA GCAGCAAAAG CAACAAAAGC GGCAGAAGCA AAGGGAAGAG TCGCCGAAGC 
AGGCCATTGT CACCAGAACT TGAGTCATCG GATGATTCCT ACGTATCGGC GGGAGAGGAT 
CCAGCAGAGG CGCCTGTGTT TGAGATTCCT TTGCAAAGTG CACTAGTAAA CGCCGGCACT 
GAAGTCTTAC TGAAATGTAT TATATCTGGA AACCCAAGTC CAGAAGTTTT TTGGAGAAAG 
GATCACGTGT TGCTGAAAAA CAGTCCGACT CATCAAATAC GAGTAGAGGG CGAGAGACAC 
ACGTTGCTAC TGCGCTGGGC ACTGCCCTCA GACTCGGGTA TCTATACAGT GACTGCGCGG 
AATGAAGTTG GGGAAGCCAG CAGCTGCGGT GCCCTCACTG TAAAACCAGC ACCTACCAAA 
GAGTCGCCGG CACATCGGGG AACACCTCGT GACCTGCTCA GCCCAATAAC ATCGGATGAA 
GAGTATCTAA GCCCCCAGGA AGATTTAAGT GAGCCTACCA CACCCCAACA TAAGATGGGA 
GGCAAAGGCC CCGCATTCAA AGCTCCACCC ACTTTCAAGA TGCCTCTTTT GGACCAGAAA 
GGGTTTGAAG GCCAAGAGAT TGTTCTCAGC GTTCAGGTGG AAGGGGAACC CAAGCCTATT 
ATCAACTGGC TGAAGAACAA GCAGCAGGTG AAGCCTGGTG GAAGGTTCCG CATATCAGAA 
GGTGCTTGGG GGATCTTCTC GCTACATATT GCCGGGGCTG ATAAACGAGA TTCCGGATTC 
TACACCTGTA AAGCAATAAA TGAGTATGGG ACAAAGCAAT GTGAAGCCAA GATAGAGGTT 
CTAGCACCCC CTGGCAGTAC CAGCTTGGAG GTTCTGGCCC CCTTGCAGGA TGTGAGTGTG 
TGCGCTGGGG AGGCAGCGGT GTTTGAATGC GTGGTGTCGG GCCCCGCCGA TATGGACGTG 
GACTGGATGT TCCGGGGCAA GCTGCTCCAG CCAGCACTGC TGGACTGTAA GATGCGTTTC 
AACGGAAAGC AGTGTGTGCT GCTGCTGAAT TCAGTCCATG AAGATGACAG TGGGGTCTAC 
ACCTGCAAAC TGAGCACTGC AAGAGATGAG CTGACCTGTA GCGCTATGCT AACTGTGCAC 
CCATCGCTGG CGCCTCTCTT CACTCGCAAG TTGTCAGATC GAGATGTTAT AGAGGGACGC 
ACAGCTCGGC TGGACTGTAA AATCAGTGGC ACCCCGCCCC CTGTTGTCAC GTGGACTCAT 
TTTGGACAGC CCATAGAGGA AAGTGAGAAC ATCCACGTTT TGAGAGAAGG CGGACATCAC 
ACCTTACTGA TACCCCACGT GGCGAATGAG GACGAGGGTC AGTACACAGC GACTGCCCAT 
AACAAGCATG GGGAGGCCGA ATGCTCTGCT GAACTCTACG TGGAGGAGCC TCGTTCTGCT 
ACAGCATCTC ATATCTCCAA GCTGGAGAAG ATGCCATCTA TTCCAGAAGA GCCAGAGATG 
CAGGAGTGTG AGGTAGAGGG TGTGATGATG CCGGATTTCC TGCGACCTCT GCAGGACATT 
GACGTGGTGG AATTTCGGGA GGTACAACTT GAGTGTCAGG TAACCGGTCT GCCGTACCCT 
ACCATCACCT GGTATCACAA TGGTCAGAAG ATCCAGAGCT CTGATGATCG CAGAATGACT 
CAATATAAAG ACATCCACCG CCTGGTTTTC CCCTCTGTTA CTCATTCTCA TGCCGGGGTC 
TACAAAAGTG TCATCTCAAA CAAAGTGGGG AAAGCAGCCT GCTATGCGCA CCTGTATGTC 
ACAGATTTGG TACCCACTCC TCCAGATGGT CCCCCCATCA TTACCTCTGT AACTGGGAGA 
ATTGTGAAGC TCCAGTGGAA GCCACCCAAG AGATTGGACC CAGCCATTGA TCTGGCTCAG 
ATTACATACT CAGTCCAGCA ACAAGCGGTG GGATCTCCCC AATGGACAGT CATCGCCACC 
AACCTAAAAG ACACTTGTTA TTGTGTCAGA TCTCTGACCA AGGAACATCA GTATCTTTTC 
CGTGTGACCA CAAACACCCC AACTTCACAC AGTAAACCTT CCCCTCCCAG TGAAAGCGTT 
TGTCTCTTTA ATAGAGGTCC CTACCTGGAG GAGATGCCTT CTATACTGGA TCGGCCTGAA 
ATCTTGTATA TGGTGGAAAA TCAGCCTCTT TGCATAACCG TGACGCTGAA CCACGTACAT 
GCCAAAGTCA CGTGGCAGAG AATAGACACC GTTCTGAGCA ATGTCGAGGG TGTGTATGAG 
CTGAGTATGC CGGATGACGA CCAGCACTCG CTCACGTTCT TCAGCCCAAG GAAGTCTGAC 
CTGGGACAGC TGACATTTGA AGCTAAAAAC CGCTACGGAT CAGATAGCTG CACTATTTCC 
ATCGAGCTCG CAGAGGCTCC TCGCTTTGAG TCCATCATGG AAGACATAGA GATCGGTGTT 
GGTGAAACAG CTCGTTTTGT GGTGGTGGTG GAAGGGAAGC CTATTCCAGA CATCATGTGG 
TACAAAGATG GGGTACTTTT GGCAGAGAGC GGTCACTTCA GCTTTGTGTA TGATGATACC 
GAGTGCTCTC TGGTGATCTT AAACACGGCA CTTGGTGACT CCGGTGTGTA CACCTGCACG 
GCACGGAACC TTTCAGGGGA AATTTCCTGC AAGGCGGAGC TGCAAGTGTG CCCAGATGAA 
CCAGGGGCAG GATCTGCAAT ACAGGAAATA GATATACTCA AAGTTCGTAG ATTGACTGAT 
TTCTACAACA TCCATAAAGA AATTGGCAGA GGGGCCTTCT CCTATGTCCG ACATGTGGTA 
GAAAAGAGCA ACGGGCGAGA TCTTGCTGCT AAATTCATAC CGTTCCGTGG GGCAACCAGG 
GAGAGTGCAC GGAGGGAGCG GGACATACTG TCCAAGCTCA GACACGACAG GATCATCTTC 
TTCTACGATG CATTCGAGAA AAGAAGTGCT CTAATCATTG TCATGGAACT CTGTTCTCAA 
GAGGAGCTTC TAGAGAGGTT GACAAGAAAG CCTACAGTGA GCGAATCAGA GATCCGCTCG 
TTTATACGGC AAATCCTAGA AGGGTTAGAT TATTTGCACC ATAAAAACAT TCTGCATTTG 
GACATAAAGC CAGAGAACAT CCTGATGGCC GATACGACTA GTGAACAGAT TCGCATTTGT 
GACTTTGGAA ACGCTCAGGA ACTAACCCCG GGGGATCCTC AGTACTGCAA ATACGGAACC 
CCGGAATTTG TGGCGCCCGA AATTGTGAAC CAGATGCCAA TTTCTACAGT AACGGACATA 
TGGCCAGTTG GAGTCCTGGC ATATTTATGC CTTACCGGAG TGTCGCCCTT TGTTGGGGAG 
AATGATTACA CCACTCTGAT GAATATCCGG GGTTACACTG TGGCATTTGA AGAGAAGATG 
TTTGTTGGTC TCACACGAGA AGCACGTGGT TTCCTAATCA AAGTCTTGGG CAATGAGAAG 
CTGAGGCCTA ATGCTGAAGA GAGTTTGGAG CACCCCTGGT TCAAAACTTT AGCGAAAGGG 
AAAAGCATCA GCACAGACCA TCTGAAGCTG TTCCAGTCTC GCCGTAAATG GCAGCGCTCA 
CTTATCAGCT ATAAGTCCAA TATGGTGATG AGAACCATTC CAGAGCTGCT TCAGGACACA 
AGCAATCACT TGTCCATTGC TGTACCCAAG AATTCCAAGG AAGCATCCGG ATTATCATCC 
TCCTCTGATT CTGATGACCT GGAAGAGCTT CCCTTTGTCC CTATGCCTCT CCAGGTCCAA 
TTCTCTGGAT CACGCATGTC CTTGAATGAG ATCCCAACAG ATGAGGAACA ACTGTCTCAG 
CCCCAGGACA ATGGCAGCTG TAGAGAAGAT GAGGAAGAAG GAAGTCAGGC AGTGGAAATT 
AGTAGCACAT TTAAAGAACA AGCAGTTCAG CAGAAAGAAG CAACAGTCCA AGCAAGTCCA 
TTGTCCAAGC ATGCCAAAGG AACCCTTACA AGGAACAGGT CTGCTGAAGT TGGTTCTTCT 
TCTGATGAGG AAACTTCAGA AACTGAAAAA AGAGAGCATC CTCGTAGGCC TCTGAAAAAA 
GGTTCCAGCT TAGAATCTGC CGACGTTCCT GATGGGGAAA TTAAAGCAGC TCGTAGAGGA 
GAGCTGAGGA GGGGAAGCTC TGCAGATAGT GCTCTTTTGC TCAATGTGTC TACAACAGAT 
GGAGATGAAG AGAGAGATCG ACCAGACAAG GGAATGATAA AAGCTGCCTC AATGGAGCTA 
CCAACCAGAT GCAGGAGCCC AGGTAGATTG GAGGATCGAG GCACACTACG AAGAAAGTTT 
GGATCAGCTG ATGAAGAATA TGCTCAGCGG TTAGAAATGA TGAGACAGAG GCTATTGAGG 
GGAGGTTCGG CAGATGGAAA ACTCAGTGGT CTTAGAGGCC CACTCATAGA AACACTTTCT 
GTAGACAAGA AGCGAGCAGA GCAGATATCT ATACGCTCCC CAAGAAGTGA TCAGTCTTCT 
CTGAGTCCTA TTCCCGCCAT CAAACTAACC CGAGCTGCTT CCAGTGAGGC TGCGCCTGGT 
AGAGAAGCAA CCGAAGAAAG AGTCTTAAGG AAAACCAGTT CCTTTTCTCA CAGCGACACA 
GAGCCACTGG TCTTGCATCG CAGGTCAGGG GCACCCCTAG AAATACCTCT TGCTCAGTTA 
GAGGCACAGC GTCTTAAAGA GTCTCCCTCT CTTTCAGCAT TGACAGACCG ATTAGAATCA 
CGTCCTCAAA CACCGCGAGA GATGATTCCC AAATCTCTAA CTCCAGAAGC ACCGCAATTG 
AAGGCAGAAA GTGGGGAGCC AGAAACTCAG AGTGATAAGG AAGCTGAGAA GGGCACCACA 
CCTCTTATTC AGCATCATAA AGACAAAGAC TCAAATTCAT TGAAGGACTT GGTTATTGTT 
CTTCCAAGAG TGACTCCACC TTTGGATAAA ATGGCATCTA AAACAGAGTT ACCAAGTATT 
CAAACAGAGA GGGCACAGGA CAAAATCCCT AGAGCAGATG TGAACCTGCC AGCTACTACT 
TCATCAAATG AAACTGTGCC AACAAAGCCA CCACCCTTAC TTTTGCCTTC TAAGCAGTCT 
CTTTCTGTGC CACCTGAACC TTTACCCTCT TCAGTACTGA CAAATGAGAT GCCTAAATCT 
AAAGAGGAGT ACTCGTCATC AAGGGCCATT AAGGCCAAGG AATCCATATC AAAAACAGAA 
ACAACAGACC AGCAAGATAA AATTAAAGAA CCTTTACCAC AAGCTGTTGT TCCATTACCA 
TTGCCTATGC CAGCCTCCCA AGTGCTAAAA CCTTCCACTG CAAAAGTACA GATAGCAGAT 
TCTCCAACAA TGCCTGAGAA TGATGCATCA CTTCCACAGA TAACAGGTAA AGAGAAACCA 
TCTTCTCCAG CTGTCCAACC AGTAGATAAA ACAGCAGCTA TATCATCACC TTATGCTGCT 
ATGATTCAAA CACTTCAAGT GCCCGAGGAA CCTGAGACAG TTGTTGAGGA ATGTTCAAAG 
GCCCCATTAA AAGCTCAGCC AGTATTTCAA GAAATTCCTA TAGCCACTGG AGATGCAAAG 
GCTCAAGGAA TATCACAAGT AAACAATAAA GAAGGCAAGG ATGAGCTATC ACAAGAACCC 
AAGCTGATTA ACACCAGATC TTCCAGCACA GAGAATGTCC CATTTATCAG CAACATTGAT 
TCAGAAGAAA TCTTTGAGGC TAAGTTCAAG AGGAACCGTG AAAATTCTCT TACCCGAGGT 
CTAAAAAAGC TTACCAGGAC TTGGTCAGAG GAGAAGAATT TAGCTGCAGC CCATGCATCT 
CGGGAAGAAG AAATGTACAG GCCTAGTCCT GTGGGTGTCC CACTAGAGTT CCTTGTTCCT 
GCAGCTCTTG GGCTACATGA AAGATCAAGA TCAGTGCAAG ATCTGCCAGA TGCTGAGAGG 
GATTCAAGCT TTATGAGAAA GCTTTCTTTG AGATTTAAGA AAACTCCACC AACTGAAAGG 
AAGCAGAAAC CAGCAGAAGA AGTAGAAGGT ACTGGTAGAC GACTTTCATG GTCAATAGGT 
AGAGGAAGTT CCAAAGACAA GAAGGATACA GAGAGCCTGA ACTCTGAAAC AGGCTCAATA 
GAAACCATCA CAGACAAACA TCTAAAGGAC CAGAAAAAGA GTAGTCAATC ACCAGTGTTG 
GCCATGAGAA AGAAAATAGG CAGTACTATG GATCGACTTT CTATGAAACT AAGGAGCCAA 
TCAGAAGATA GAAGGGATGC AGAGAGAACA GAGAAGAAAG AAGGGCGTAC AGACAAAAGC 
TCTCCATTAA TGTCTTTACT TAGGAGATCC AACTCAGAAG GAGAGAACTT AAAGAAACTA 
GGTATTCCCC AGAACCAGCT TGCTTCCCAG GTTTCAGCTC CATCAACTGA GTCTATTGAA 
TCTGGTCTCA GCATCAAATC TGAAATGGTA GTCAAGGATG ATAGGCGCTC AAGGTGGGAT 
CGTTGGGGCC TGTCACGTAG CAAAAAGGAG AAGATGACAT CGCAGCCAAG TATTCCAGCC 
AGTCTGATGC ATGAAGATGG CAGTATTGTT GGCAGACAAT ACATTCGTAA TGAGTCAGAC 
TTCCCCCCAG TCTTCCATAT CAAGCTGAAG GACATCGTGG TGCTAGAGGG AGAGCCGGTG 
ACTCTGTCCT GCTTACCTGC TGGCAGCCCT TCCCCCCAGA TCCTGTGGAA GAAAGATAAG 
ATGTCCTTGG AAAGAGGAGA AAAGGTGCAT ATAAAATCCT GCCCCGATGG CAGACAGCAG 
CTCATTATTC CCGCCGCCGG GCAGAGGGAA GCCGGTCTGT ATGAATGCGT AGCAGCCAAT 
GCCCTGGGCT CAGTGGCCAG CTCCTGCACA GTGTCTGTGG CAAGAATACC TGATCCCCCG 
GGGACCCCAG AGATCCCACA GAAGTATAAG AACACGGTGC TGGTTCTATG GAAGACCCAC 
GAGCACAACT CCCCTTGCAC CTACATCCTG GAGAAACAGG TTAATGGTGA TGGGGAATGG 
AAGGTTCTAA GTTCTGGAAT CACCGACTGT TACTTCAATG TGACGGAGCT CCAGGAAGGC 
ACTGTCAGGT TCCGCGTCGC TTGTGCCAAC AAAGCAGGAA AGGGGCCCTA CAGCAATGTG 
TCCAAGACAA TCGTCGTAGA TGCAGAAGAT CGTAAACCAG GAACACCCTC TGTCCAGAAG 
GCACGGCCCG TGGGAGCTGC AGTGTCCACT ACTGTTCCAA TGACTCTCCG GACCCCTTCT 
ACCCCAGCTG TTGCGCCCCC TGTTGTCTCT ACCCCCAAAT TACCTCCATC TTGTGGATCT 
GCTCTTGCAG TTTCCTCAAC AGTTATCACT ACAACCGCAA CTGTGTCTCC AGCCCAGCCT 
CCCACCCCTC CTACCCCCAT TAGCCCCACT TCCCCAACCG CCCCATTTAC CCCCACTTCC 
CAAACTGTCC CTACACGGGT GATAGTAAGA AGTGTCACCC CCGGGAAGGA TGCACGTTAT 
ACACCCGGAG GAAGAGTCAC ACCCTCGGGG AGAGAAAGCA CCACCCTCCG ACAAGGGGTA 
CCCCAAAAAC CTTACACTTT CTTGGATGAG AAAGCAAGGG GGCGGTTTGG AGTGATCCGA 
GAGTGTAAGG AGAATGCAAC TGGGAAGCAC TTCATGGCAA AGATAATCCA GTACGAACCA 
GAAAACAAGC AAAACGTGCT GCAGGAGTAT GAGATCCTCA AGGGTCTCCA CCATCAACGA 
ATCCTGAGTC TTCATGAAGC TTATATTACC CCTCGCTACC TTGTGTTGAT TTCTGAAAAC 
TGCACAGGAC GGGAACTGCT TTACTGCCTA GTTGAGAGGT TCCGTTATTC TGAAGACGAC 
GTGGTCAATT ACCTCCTGCA GATCCTACAG GGGTTGGAGT ACCTCCATGA ACAAAAGGTC 
CTGCACCTCG ATATCAAACC TGAGAACGTC ATTGTGTCCT ACATGAACAC TGTCAAGATC 
ATCGATTTGG GGAGTGCCCA GAACTTTACA CCGCTGGTCC TGCGACCCCT CGGCAAACGC 
ATAGGGACCC TGGAATATAT GTCCCCCGAA ATGCTCAAAG GAGACGCAGT AGGCCCAGCA 
GCTGATATTT GGGGTGTAGG TGTTCTGACG TACATAATGC TCAGCGGCCG CTCTCCGTTC 
TTTGAGCTGG ACCCCGTGGA AACCGAGCAC AAGATCTTGT CGGGACGATT TGATATCTTT 
AAATTGTATT CCAACGCCTC CCAAAGTGCT TCCCTCTTCA TTCGCAAGAT CCTGACTATA 
TATCCTTGGA GCCGCCCATC ACTTAAGGAA TGTTTTTCCA ATCCCTGGCT CCAGGATGCC 
TACCTAATGA AGCTTAGACG CCAAACCCTA ACCTTCACCA CTAACAAGCT CAAAGAGTTC 
CTAGTGGAGC AACAGAGGAG ACGCGCAGAC ACAGCCACTA AGCACAAAGT CCTCCTTCGT 
TCGTACCACG GAAGCCAGCC CTCAGCTCCT GTCACTCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

speg ( XM_031892843.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031748703.1 Xenopus tropicalis striated muscle enriched protein kinase (speg), transcript variant X1, mRNA.

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CloneID	OXa66222
Clone ID Related Accession (Same CDS sequence)	XM_031892843.1
Accession Version	XM_031892843.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11427bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	striated muscle preferentially expressed protein kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGTG CCTATGGGAG GAGGAACCTG GCCCGGAATG AAGAGGAAGC CCCTGTCTCC 
CCAACTCAAA CGTTTAGGTC TCCACCAAGC CCAGGCATTC CCCCAAAACA CCCCAAGTTG 
GCGGCTGAAC CTGGGCAGCC CTCTCCCCCT CAGTTTGTCC GGAAGCTGAA AAATGCTGCT 
ATAGGCACAG GCTGTGACAT CTGCCTAAAG GTGGCAGTGG CAGGGCACCC GCTGCCAACC 
CTAAACTGGT ACAAGAACCA GGAACGCATC CAACCCGGAG GAGAGGAATA TGGGACACTA 
TGCATCAGGG ACAGCAGGAT GGAAGATGCT GGAGTGTACA CCTGCGTGGC CCAGAATCCC 
CTGGGGGAGG CTATTACCTC TGCTGTGCTG GCAATCATTG AATTAGAAGA CTCAGAACCA 
GGGGAAGAAG ATGCAGGGGG CCCCCAATTA CAACGCACAA TTCGGGCACC CAACAATACG 
CCTCTGGGAA CCCCTTCAGG GGATTCAGAT ACGCTGCTGG CGAGCTCCCT GCACACCACG 
CCCAGCACGC TGACTGCACT CTCTCAAACA GACGAGCTCT CCGATTGGTC AGGCAGCCAA 
CAGACAGTAC TAGAAAGGGA CCCAAGGAAT GCAAGGCAAG GCTTCACTCA GGGAGGAGGG 
GCTCGCCATC TGGGGGTGGA GCCCCTCATT CCAGCTTCCA GCAACGAGTT CCCGGGCAGT 
GTCTGGGGGT CAGAGGTCAG CCTTTCTGGG TTCAGTGATC TGTATAGCAG CGCCTTCAGC 
CTGTACCGAG GCAGATCTTG TGCCATCCGG GTGTTCCTTC CGGAAGGGGG TCTGCGGAGG 
GGTAGCATCA ATGGTTCTGT AACAGACTCC CCACGAAACT TTGTCAATCC TAGGCCGCCT 
ATCATACCAC CACCCTCTCC GCGCATTGGC CAGCGTCCAG CTGTCATTCC TGCAGGCCGT 
GGACCCCCCA CCCCTGTTAC ACCTAGAAAG AAAACCACAA TGCCCAACCA GTACCAGGAC 
ACAGTGACTG AGGAGTTAGA AGAGAAGATT AAGAAGCCAA AGTCCTCTGT TGGCTCTCAG 
GCTAGTGGCC AAGATTCTCG CCCTGTAACT CCACTAAGTG AGACATCAGG GCGGGTGTCT 
ATCCTTAGGG CATCCCCAAA GCTAGTCCGC TCAGGCTCTA AGATCTTTGA AAAGCTTAGG 
TGTCTCGAAG AGAGGAGAAA AAGTCTGGAC CAGACAGACA GCCCCTTTCC AGTGCACTCA 
TGGTTGCCTC TCCGTAAGGC ACGATCCTTT GATCAGCCGG GGGCTGAGGG GCTTGGTGCT 
GGTCTAGGTT CCTCAAACGA AGAGCTAAGG GATGATCTTA GAGACGGGGT AAGATCAGAA 
ATAGGTGGGT TTACTCTGAG GTACCCCTCT CTGAGACACA AGACTACCTC ACTGGATGAC 
AGGGGAGCTC TCTATGGACG AATAAGTGAC ATTGAGACCC GCTTCTCACA AGAGCTGACT 
CGCATCAAAA GAACTGTGTC CCAGCAACAG CTAGTCCGTT CCTCACAGGA TCTATCTGGA 
AGATCACCGT CACCCCAAAG ATCCCTAGCT GCCCAGCCTG CTCCTGTGGA CCCTAAGCTA 
ACCAAAGTGT CAACGGCTCC AAAGCCTTCT GAAACAGAAG AGAAGCAGCC AGCAAGGAAG 
CCACTAGTCA GAAGCTTTGC CACCGTCAAC CAACTGTCAG AAATTAAGGA ACCTCTGGGG 
TTTTCAAGCC AACCCCCTAC ACTGCCATCA AAATCATCCA ATGGTTCTGC AGTATATCAG 
GATGCAGTGA GGCACTCGGA AACAAATATG AAATCACAAC CACACCTTAC AGGAAAAATC 
ATTGCACCAA AAACTGTTTC CCCACAAGTA TTTAAAGCTA TGACTCTCCC CGCACAATCC 
CAAAAAGAGG CAGTCGGCGA CACACAGCCG AAGAGAGTTC CTGATGTTGG TAGCGTGCCT 
CCGCCACCCA GCACACATGC CCCGGCAGCC CTAGAGATAG CTCAGAGGGG ACTTGTTAAA 
GAGAAGGGGA CTGTAAGTGA AGAGAAGAAG TATCTACCCT GGGCACTTCC TGTAGGAGAC 
AATAACAACA GCCAAATGCC ACAGGGTAAG GGTGGGGGAC AGGCCAAAAA GCACAGCGAT 
GCACACACCA GCAGCAAAAG CAACAAAAGC GGCAGAAGCA AAGGGAAGAG TCGCCGAAGC 
AGGCCATTGT CACCAGAACT TGAGTCATCG GATGATTCCT ACGTATCGGC GGGAGAGGAT 
CCAGCAGAGG CGCCTGTGTT TGAGATTCCT TTGCAAAGTG CACTAGTAAA CGCCGGCACT 
GAGGTCTTAC TGAAATGTAT TATATCTGGA AACCCAAGTC CAGAAGTGTT TTGGAGAAAG 
GATCACGTGT TGCTGAAAAA CAGTCCGACC CATCAAATAC GAGTAGAGGG CGAGAGACAC 
ACGTTGCTAC TGCGCTGGGC ACTGCCCTCA GACTCGGGTA TCTATACAGT GACTGCGCGG 
AATGAAGTTG GGGAAGCCAG CAGCTGCGGT GCCCTCACTG TAAAACCAGC ACCTACCAAA 
GAGTCGCCGG CACATCGGGG AACACCTCGT GACCTGCTCA GCCCAATAAC ATCGGATGAA 
GAGTATCTAA GCCCCCAGGA AGATTTAAGT GAGCCTACCA CCCCCCAACA TAAGATGGCA 
GGCAAAGGCC CCGCATTCAA AGCTCCACCC ACTTTCAAGA TGCCTCTTTT GGACCAGAAA 
GGGTTTGAAG GCCAAGAGAT TGTTCTCAGC GTTCAGGTGG AAGGGGAACC CAAGCCTATT 
ATCAACTGGC TGAAGAACAA GCAGCAGGTG AAGCCTGGTG GAAGGTTCCG CATATCAGAA 
GGTGCTTGGG GGATCTTCTC GCTACATATT GCCGGGGCTG ATAAACGAGA TTCCGGATTC 
TACACCTGTA AAGCAATAAA TGAGTATGGG ACAAAGCAAT GTGAAGCCAA GATAGAGGTT 
CTAGCACCCC CTGGCAGTAC CAGCTTGGAG GTTCTGGCCC CCTTGCAGGA TGTGAGTGTG 
TGCGCTGGGG AGGCAGCGGT GTTTGAATGC GTGGTGTCGG GCCCCGCCGA TATGGACGTG 
GACTGGATGT TCCGGGGCAA GCTGCTCCAG CCAGCACTGC TGGACTGTAA GATGCGTTTC 
AACGGAAAGC AGTGTGTGCT GCTGCTGAAT TCAGTCCATG AAGATGACAG TGGGGTCTAC 
ACCTGCAAAC TGAGCACTGC AAGAGATGAG CTGACCTGTA GCGCTATGCT AACTGTGCAC 
CCATCGCTGG CGCCTCTCTT CACTCGCAAG TTGTCAGATC GAGATGTTAT AGAGGGACGC 
ACAGCTCGGC TGGACTGTAA AATCAGTGGC ACCCCGCCCC CTGTTGTCAC GTGGACTCAT 
TTTGGACAGC CCATAGAGGA AAGTGAGAAC ATCCACGTTT TGAGAGAAGG TGGACATCAC 
ACCTTACTGA TACCCCACGT GGCGAATGAG GACGAGGGTC AGTACACAGC GACTGCCCAT 
AACAAGCATG GGGAGGCCGA ATGCTCTGCT GAACTCTACG TGGAGGAGCC TCGTTCTGCT 
ACAGCATCTC ATATCTCCAA GCTGGAGAAG ATGCCATCTA TTCCAGAAGA GCCAGAGATG 
CAGGAGTGTG AGGTAGAGGG TGTGATGATG CCGGATTTCC TGCGACCTCT GCAGGACATT 
GACGTGGTGG AATTTCGGGA GGTACAACTT GAGTGTCAGG TAACCGGTCT GCCGTACCCT 
ACCATCACCT GGTATCACAA TGGTCAGAAG ATCCAGAGCT CTGATGATCG CAGAATGACT 
CAATATAAAG ACATCCACCG CCTGGTTTTC CCCTCTGTTA CTCATTCTCA TGCCGGAGTC 
TATAAAAGTG TCATCTCAAA CAAAGTGGGG AAAGCAGCCT GCTATGCGCA CCTGTATGTC 
ACAGATTTGG TACCCACTCC TCCAGATGGT CCCCCCATCA TTACCTCTGT AACTGGGAGA 
ATTGTGAAGC TCCAGTGGAA GCCACCCAAG AGATTGGACC CAGCCATTGA TCTGGCTCAG 
ATTACATACT CAGTCCAGCA GCAAGCGGTG GGATCTCCCC AATGGACAGT CATCACCACC 
AACCTAAAAG ACACTTGTTA TTGTGTCAGA TCTCTGACCA AGGAACATCA GTATCTTTTC 
CGTGTGACCA CAAACACCCC AACTTCTCAC AGTAAACCTT CCCCTCCCAG TGAAAGCGTT 
TGTCTCTTTA ATAGAGGTCC CTACCTGGAG GAGATGCCTT CTATACTGGA TCGGCCTGAA 
ATCTTGTATA TGGTGGAAAA TCAGCCTCTT TGCATAACCG TGACACTGAA CCACGTACAT 
GCCAAAGTCA CGTGGCAGAG AATAGACACC GTTCTGAGCA ATGTCGAGGG TGTGTATGAG 
CTGAGTATGC CGGATGACGA CCAGCACTCG CTCACGTTGT TCAGCCCAAG GAAGTCTGAC 
CTGGGGCAGC TGACATTTGA GGCTAAAAAC CGCTACGGAT CAGATAGCTG CACTATTTCC 
ATCGAGCTCG CAGAGGCTCC TCGCTTTGAG TCCATCATGG AAGACATAGA GATCGGTGTT 
GGTGAAACAG CTCGTTTTGT GGTCGTGGTG GAAGGGAAGC CTATTCCAGA CATCATGTGG 
TACAAAGATG GGGTACTTTT GGCAGAGAGC GGTCACTTCA GCTTTGTGTA TGATGATACC 
GAGTGCTCTC TGGTGATCTT AAACACGGCA CTTGGTGACT CCGGTGTGTA CACCTGCACG 
GCACGGAACC TTTCAGGGGA AATTTCCTGC AAAGCGGAGC TGCAAGTGTG CCCAGATGAA 
CCAGGGGCAG GATCTGCAAT ACAGGAAATA GATATACTCA AAGTTCGTAG ATTGACTGAT 
TTCTACAACA TCCATAAAGA AATTGGCAGA GGGGCCTTCT CCTATGTCCG ACATGTGGTA 
GAAAAGAGCA ACGGGCGAGA TCTTGCTGCT AAATTCATAC CGTTCCGTGG GGCAACCAGG 
GAGAGTGCAC GGAGGGAGCG GGACATACTG TCCAAGCTCA GACACGACAG GATCATCTTC 
TTCTACGATG CATTTGAGAA AAGAAGTGCT CTAATCATTG TCATGGAACT CTGTTCTCAA 
GAGGAGCTCC TAGAGAGGTT GACAAGAAAG CCTACAGTGA GCGAATCAGA GATCCGCTCG 
TTTATACGGC AAATCCTAGA AGGGTTAGAT TATTTGCACC ATAAAAACAT TCTGCATTTG 
GACATAAAGC CAGAGAACAT CCTGATGGCC GATACGACTA GTGAACAGAT TCGCATTTGT 
GACTTTGGAA ACGCTCAGGA ACTAACCCCG GGGGATCCTC AGTACTGCAA ATACGGAACC 
CCGGAATTTG TGGCGCCCGA AATTGTGAAC CAGATGCCAA TTTCTACAGT AACGGACATA 
TGGCCAGTTG GAGTCCTGGC ATATTTATGC CTTACCGGAG TGTCGCCCTT TGTTGGGGAG 
AATGATTACA CCACTCTGAT GAATATCCGG GGTTACACTG TGGCATTTGA AGAGAAGATG 
TTTGTTGGTC TCACACGAGA AGCACGTGGT TTCCTAATCA AAGTCTTGGG CAATGAGAAG 
CTGAGGCCTA ATGCTGAAGA GAGTTTGGAG CACCCCTGGT TCAAAACTTT AGCGAAAGGG 
AAAAGCATCA GCACAGACCA TCTGAAGCTG TTCCAGTCTC GCCGTAAATG GCAGCGCTCA 
CTTATCAGCT ATAAGTCCAA TATGGTGATG AGAACCATTC CAGAGCTGCT TCAGGACACA 
AGCAATCACT TGTCCATTGC TGTACCCAAG AATTCCAAGG AAGCATCCGG ATTGTCATCC 
TCCTCTGATT CTGATGACCT GGAAGAGCTT CCCTTTGTCC CTATGCCTCT CCAGGTCCAA 
TTCTCTGGAT CACGCATGTC CTTGAATGAG ATCCCAACAG ATGAGGAACA ACTGTCTCAG 
CCCCAGGACA ATGGCAGCTG TAGAGAAGAT GAGGAAGAAG GAAGTCAGGC AGTGGAAATT 
AGTAGCACAT TTAAAGAACA AGCAGTTCAG CAGAAAGAAG CAACAGTCCA AGCAAGTCCA 
TTGTCCAAGC ATGCCAAAGG AACCCTTACA AGGAACAGGT CTGCTGAAGT TGGTTCTTCT 
TCTGATGAGG AAACTTCAGA AACTGAAAAA AGAGAGCATC CTCGTAGGCC TCTGAAAAAA 
GGTTCCAGCT TAGAATCTGC CGACGTTCCT GATGGGGAAA TTAAAGCAGC TCGTAGAGGA 
GAGCTGAGGA GGGGAAGCTC TGCAGATAGT GCTCTTTTGC TCAATGTGTC TACAACAGAT 
GGAGATGAAG AGAGAGATCG ACCAGACAAG GGAATGATAA AAGCTGCCTC AATGGAGCTA 
CCAACCAGAT GCAGGAGCCC AGGTAGATTG GAGGATCGAG GCACACTACG AAGAAAGTTT 
GGATCAGCTG ATGAAGAATA TGCTCAGCGG TTAGAAATGA TGAGACAGAG GCTATTGAGG 
GGAGGTTCGG CAGATGGAAA ACTCAGTGGT CTTAGAGGCC CACTCATAGA AACACTTTCT 
GTAGACAAGA AGCGAGCAGA GCAGATATCT ATACGCTCCC CAAGAAGTGA TCAGTCTTCT 
CTGAGTCCTA TTCCCGCCAT CAAACTAACC CGAGCTGCTT CCAGTGAGGC TGCGCCTGGT 
AGAGAAGCAA CTGAAGAAAG AGTCTTAAGG AAAACCAGTT CCTTTTCTCA CAGCGACACA 
GAGCCACTGG TCTTGCATCG CAGGTCAGGG GCACCCCTAG AAATACCTCT TGCTCAGTTA 
GAGGCACAGC GTCTTAAAGA GTCTCCCTCT CTTTCAGCAT TGACAGACCG ATTAGAATCA 
CGTCCTCAAA CACCGCGAGA GATGATTCCC AAATCTCTAA CTCCAGAAGC ACCGCAATTG 
AAGGCAGAAA GTGGGGAGCC AGAAACTCAG AGTGATAAGG AAGCTGAGAA GGGCACCACA 
CCTCTTATTC AGCATCATAA AGACAAAGAC TCAAATTCAT TGAAGGACTT GGTTATTGTT 
CTTCCAAGAG TGACTCCACC TTTGGATAAA ATGGCATCTA AAACAGAGTT ACCAAGTATT 
CAAACAGAGA GGGCACAGGA CAAAATCCCT AGAGCAGATG TGAACCTGCC AGCTACTACT 
TCATCAAATG AAACTGTGCC AACAAAGCCA CCACCCTTAC TTTTGCCTTC TAAGCAGTCT 
CTTTCTGTGC CACCTGAACC TTTACCCTCT TCAGTACTGA CAAATGAGAT GCCTAAATCT 
AAAGAGGAGT ACTCGTCATC AAGGGCCATT AAGGCCAAGG AATCCATATC AAAAACAGAA 
ACAACAGACC AGCAAGATAA AATTAAAGAA CCTTTACCAC AAGCTGTTGT TCCATTACCA 
TTGCCTATGC CAGCCTCCCA AGTGCTAAAA CCTTCCACTG CAAAAGTACA GATAGCAGAT 
TCTCCAACAA TGCCTGAGAA TGATGCATCA CTTCCACAGA TAACAGGTAA AGAGAAACTA 
TCTTCTCCAG CTGTCCAACC AGTAGATAAA ACAGCAGCTA TATCATCACC TTATGCTGCT 
ATGATTCAAA CACTTCAAGT GCCCGAGGAA CCTGAGACAG TTGTTGAGGA ATGTTCAAAG 
GCCCCATTAA AAGCTCAGCC AGTATTTCAA GAAATTCCTA TAGCCACTGG AGATGCAAAG 
GCTCAAGGAA TATCACAAGT AAACAATAAA GAAGGCAAGG ATGAGCTATC ACAAGAACCC 
AAGCTGATTA ACACCAGATC TTCCAGCACA GAGAATGTCC CATTTATCAG CAACATTGAT 
TCAGAAGAAA TCTTTGAGGC TAAGTTCAAG AGGAACCGTG AAAATTCTCT TACCCGAGGT 
CTAAAAAAGC TTACCAGGAC TTGGTCAGAG GAGAAGAATT TAGCTGCAGC CCATGCATCT 
CGGGAAGAAG AAATGTACAG GCCTAGTCCT GTGGGTGTCC CACTAGAGTT CCTTGTTCCT 
GCAGCTCTTG GGCTACATGA AAGATCAAGA TCAGTGCAAG ATCTGCCAGA TGCTGAGAGG 
GATTCAAGCT TTATGAGAAA GCTTTCTTTG AGATTTAAGA AAACTCCACC AACTGAAAGG 
AAGCAGAAAC CAGCAGAAGA AGTAGAAGGT ACTGGTAGAC GACTTTCATG GTCAATAGGT 
AGAGGAAGTT CCAAAGATAA GAAGGATACA GAGAGCCTGA ACTCTGAAAC AGGCTCAATA 
GAAACCATCA CAGACAAACA TCTAAAGGAC CAGAAAAAGA GTAGTCAATC ACCAGTGTTG 
GCCATGAGAA AGAAAATAGG CAGTACTATG GATCGACTTT CTATGAAACT AAGGAGCCAA 
TCAGAAGATA GAAGGGATGC AGAGAGAACA GAGAAGAAAG AAGGGCGTAC AGACAAAAGT 
TCTCCATTAA TGTCTTTACT TAGGAGATCC AACTCAGAAG GAGAGAACTT AAAGAAACTA 
GGTATTCCCC AGAACCAACT TGCTTCCCAG GTTTCAGCTC CATCAACTGA GTCTATTGAA 
TCTGGTCTCA GCATCAAATC TGAAATGGTA GTCAAGGATG ATAGGCGCTC AAGGTGGGAT 
CGTTGGGGCC TGTCACGTAG CAAAAAGGAG AAGATGACAT CGCAGCCAAG TATTCCAGCC 
AGTCTGATGC ATGAAGATGG CAGTATTGTT GGCAGACAAT ACATTCGTAA TGAGTCAGAC 
TTCCCCCCAG TCTTCCATAT CAAGCTGAAG GACATCGTGG TGCTAGAGGG AGAGCCGGTG 
ACTCTGTCCT GCTTACCTGC TGGCAGCCCT TCCCCCCAGA TCCTGTGGAA GAAAGATAAG 
ATGTCCTTGG AAAGAGGAGA AAAGGTGCAT ATAAAATCCT GCCCCGATGG CAGACAGCAG 
CTCATTATTC CCGCCGCCGG GCAGAGGGAA GCCGGTCTGT ATGAATGCGT AGCAGCCAAT 
GCCCTGGGCT CAGTGGCCAG CTCCTGCACA GTGTCTGTGG CAAGAATACC TGATCCCCCG 
GGGACCCCAG AGATCCCACA GAAGTATAAG AACACGGTGC TGGTTCTATG GAAGACCCAC 
GAGCACAACT CCCCTTGCAC CTACATCCTG GAGAAACAGG TTAATGGTGA TGGGGAATGG 
AAGGTTCTAA GTTCTGGAAT CACCGACTGT TACTTCAATG TGACGGAGCT CCAGGAAGGC 
ACTGTCAGGT TCCGCGTCGC TTGTGCCAAC AAAGCAGGAA AGGGGCCCTA CAGCAATGTG 
TCCAAGACAA TTGTCGTAGA TGCAGAAGAT CGTAAGCCAG GAACACCCTC TGTCCAGAAG 
GCACGGCCCG TGGGAGCTGC AGTGTCCACT ACTGTTCCAA TGACTCTCCG GACCCCTTCT 
ACCCCAGCTG TTGCGCCCCC TGTTGTCTCT ACCCCCAAAT TACCTCCATC TTGTGGATCT 
GCTCTTGCAG TTTCCTCAAC AGTTATCACT ACAACCGCAA CTGTGTCTCC AGCCCAGCCT 
CTCCCCAAAG GGCCTCCACC TGCAACTCCT CCACGGAAAC ACCGAGGGAT ACTCTCCACT 
CCCCCAACCA TGCACAAGCC TGTCATTGCC ACCACTCTCA GTGCCGATAT TACTCCCCAA 
ACTCTGCCCA ATAACAGGGC TCCATCTTCT GTGGCTCCTC CTGCCGTATG TTCTCATGTC 
TCCACGGTGA CTGTGTCTAT GTCACCAGGT TCTTCGGTGA CCCATTTAAG GGCCCCCTTC 
TCTACCCCCA TGCAGAAACC TACCCCAGAA CCAATAACAA AACCATTACC AGTGTCACAA 
GTACAATCAC CCAAAGCTCC TTCTGTGCCA ACAGAGCAAA CCCAGAAAAT TGCCCTTGCC 
CCGGCTGTTG CCCAGGTTAA AGCACCTTTT GTGCCAACTA CTGCAAAAAC TAACGAACCA 
TCACGTCTTC CATATAGTGT CCCTACGGAA CCACAGATGC CATCACAGTT AGCAGCTCCA 
AAACTATCTG CCAAGTCCCC CTCTGTTCCA ATTATGCTGC ACATTCCCCC CTTTAAATCA 
GCAACCCTAC CTGCTTCCAG TCCCACTTCC AAAACCATAT CTTTTCCCTC TCCTCCTACA 
AGCCCAGTAA CCAAACCCTT AACACCTACC TCCCCAGCTT ATATGGTCAC TTCCTTTATA 
TCAATGCCTG TGCCTTCACC CTCTGTTGAG TCCTCCACTC CACCTACTCC ACTTACCCCC 
ATCACCCCAC CTACCCCCAT CACCCCACCT ACCCCACTTA CCCCCACCAC CCCTCCTACC 
CCCATTAGCC CCACTTCCCC AACCGCCCCA TTTACCCCCA CTTCCCAAAC TGTCCCTACA 
CGGGTGATAG TAAGAAGTGT CACCCCCGGG AAGGATGCAC GTTATACACC CGGAGGAAGA 
GTCACACCCT CGGGGAGAGA AAGCACCACC CTCCGACAAG GGGTACCCCA AAAACCTTAC 
ACTTTCTTGG ATGAGAAAGC AAGGGGGCGG TTTGGAGTGA TCCGAGAGTG TAAGGAGAAT 
GCAACTGGGA AGCACTTCAT GGCAAAGATA ATCCAGTACG AACCAGAAAA CAAGCAAAAC 
GTGCTGCAGG AGTATGAGAT CCTCAAGGGT CTCCACCATC AACGAATCTT GAGTCTTCAT 
GAAGCTTATA TTACCCCTCG CTACCTTGTG TTGATTTCTG AAAACTGCAC AGGACGGGAA 
CTGCTTTACT GCCTAGTTGA GAGGTTCCGT TATTCTGAAG ACGACGTGGT CAATTACCTC 
CTGCAGATCC TACAGGGGTT GGAGTACCTC CATGAACAAA AGGTCCTGCA CCTCGATATC 
AAACCTGAGA ACGTCATTGT GTCCTACATG AACACTGTCA AGATCATCGA TTTGGGGAGT 
GCCCAGAACT TTACACCGCT GGTCCTGCGA CCCCTCGGCA AACGCATAGG GACCCTGGAA 
TATATGTCCC CCGAAATGCT CAAAGGTGAC GCAGTAGGCC CAGCAGCTGA TATTTGGGGT 
GTAGGCGTTC TGACTTACAT AATGCTCAGC GGCCGCTCTC CGTTCTTTGA GCTGGACCCC 
GTGGAAACCG AGAACAAGAT CTTGTCGGGA CGATTTGATA TCTTTAAATT GTATTCCAAC 
GCCTCCCAAA GTGCTTCCCT CTTCATTCGC AAGATCCTGA CTATATATCC TTGGAGCCGC 
CCATCACTTA AGGAATGTTT TTCCAATCCC TGGCTCCAGG ATGCCTACCT AATGAAGCTT 
AGACGCCAAA CCCTAACCTT CACCACTAAC AAGCTCAAAG AGTTCCTCGT GGAGCAACAG 
AGGAGACGCG CAGACACAGC CACTAAGCAC AAAGTCCTCC TTCGTTCGTA CCACGGAAGC 
CAGCCCTCAG CTCCTGTCAC TCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031748703.1
CDS	445..11871
Translation	MHRAYGRRNLARNEEEAPVSPTQTFRSPPSPGIPPKHPKLAAEPGQPSPPQFVRKLKNAAIGTGCDICLKVAVAGHPLPTLNWYKNQERIQPGGEEYGTLCIRDSRMEDAGVYTCVAQNPLGEAITSAVLAIIELEDSEPGEEDAGGPQLQRTIRAPNNTPLGTPSGDSDTLLASSLHTTPSTLTALSQTDELSDWSGSQQTVLERDPRNARQGFTQGGGARHLGVEPLIPASSNEFPGSVWGSEVSLSGFSDLYSSAFSLYRGRSCAIRVFLPEGGLRRGSINGSVTDSPRNFVNPRPPIIPPPSPRIGQRPAVIPAGRGPPTPVTPRKKTTMPNQYQDTVTEELEEKIKKPKSSVGSQASGQDSRPVTPLSETSGRVSILRASPKLVRSGSKIFEKLRCLEERRKSLDQTDSPFPVHSWLPLRKARSFDQPGAEGLGAGLGSSNEELRDDLRDGVRSEIGGFTLRYPSLRHKTTSLDDRGALYGRISDIETRFSQELTRIKRTVSQQQLVRSSQDLSGRSPSPQRSLAAQPAPVDPKLTKVSTAPKPSETEEKQPARKPLVRSFATVNQLSEIKEPLGFSSQPPTLPSKSSNGSAVYQDAVRHSETNMKSQPHLTGKIIAPKTVSPQVFKAMTLPAQSQKEAVGDTQPKRVPDVGSVPPPPSTHAPAALEIAQRGLVKEKGTVSEEKKYLPWALPVGDNNNSQMPQGKGGGQAKKHSDAHTSSKSNKSGRSKGKSRRSRPLSPELESSDDSYVSAGEDPAEAPVFEIPLQSALVNAGTEVLLKCIISGNPSPEVFWRKDHVLLKNSPTHQIRVEGERHTLLLRWALPSDSGIYTVTARNEVGEASSCGALTVKPAPTKESPAHRGTPRDLLSPITSDEEYLSPQEDLSEPTTPQHKMAGKGPAFKAPPTFKMPLLDQKGFEGQEIVLSVQVEGEPKPIINWLKNKQQVKPGGRFRISEGAWGIFSLHIAGADKRDSGFYTCKAINEYGTKQCEAKIEVLAPPGSTSLEVLAPLQDVSVCAGEAAVFECVVSGPADMDVDWMFRGKLLQPALLDCKMRFNGKQCVLLLNSVHEDDSGVYTCKLSTARDELTCSAMLTVHPSLAPLFTRKLSDRDVIEGRTARLDCKISGTPPPVVTWTHFGQPIEESENIHVLREGGHHTLLIPHVANEDEGQYTATAHNKHGEAECSAELYVEEPRSATASHISKLEKMPSIPEEPEMQECEVEGVMMPDFLRPLQDIDVVEFREVQLECQVTGLPYPTITWYHNGQKIQSSDDRRMTQYKDIHRLVFPSVTHSHAGVYKSVISNKVGKAACYAHLYVTDLVPTPPDGPPIITSVTGRIVKLQWKPPKRLDPAIDLAQITYSVQQQAVGSPQWTVITTNLKDTCYCVRSLTKEHQYLFRVTTNTPTSHSKPSPPSESVCLFNRGPYLEEMPSILDRPEILYMVENQPLCITVTLNHVHAKVTWQRIDTVLSNVEGVYELSMPDDDQHSLTLFSPRKSDLGQLTFEAKNRYGSDSCTISIELAEAPRFESIMEDIEIGVGETARFVVVVEGKPIPDIMWYKDGVLLAESGHFSFVYDDTECSLVILNTALGDSGVYTCTARNLSGEISCKAELQVCPDEPGAGSAIQEIDILKVRRLTDFYNIHKEIGRGAFSYVRHVVEKSNGRDLAAKFIPFRGATRESARRERDILSKLRHDRIIFFYDAFEKRSALIIVMELCSQEELLERLTRKPTVSESEIRSFIRQILEGLDYLHHKNILHLDIKPENILMADTTSEQIRICDFGNAQELTPGDPQYCKYGTPEFVAPEIVNQMPISTVTDIWPVGVLAYLCLTGVSPFVGENDYTTLMNIRGYTVAFEEKMFVGLTREARGFLIKVLGNEKLRPNAEESLEHPWFKTLAKGKSISTDHLKLFQSRRKWQRSLISYKSNMVMRTIPELLQDTSNHLSIAVPKNSKEASGLSSSSDSDDLEELPFVPMPLQVQFSGSRMSLNEIPTDEEQLSQPQDNGSCREDEEEGSQAVEISSTFKEQAVQQKEATVQASPLSKHAKGTLTRNRSAEVGSSSDEETSETEKREHPRRPLKKGSSLESADVPDGEIKAARRGELRRGSSADSALLLNVSTTDGDEERDRPDKGMIKAASMELPTRCRSPGRLEDRGTLRRKFGSADEEYAQRLEMMRQRLLRGGSADGKLSGLRGPLIETLSVDKKRAEQISIRSPRSDQSSLSPIPAIKLTRAASSEAAPGREATEERVLRKTSSFSHSDTEPLVLHRRSGAPLEIPLAQLEAQRLKESPSLSALTDRLESRPQTPREMIPKSLTPEAPQLKAESGEPETQSDKEAEKGTTPLIQHHKDKDSNSLKDLVIVLPRVTPPLDKMASKTELPSIQTERAQDKIPRADVNLPATTSSNETVPTKPPPLLLPSKQSLSVPPEPLPSSVLTNEMPKSKEEYSSSRAIKAKESISKTETTDQQDKIKEPLPQAVVPLPLPMPASQVLKPSTAKVQIADSPTMPENDASLPQITGKEKLSSPAVQPVDKTAAISSPYAAMIQTLQVPEEPETVVEECSKAPLKAQPVFQEIPIATGDAKAQGISQVNNKEGKDELSQEPKLINTRSSSTENVPFISNIDSEEIFEAKFKRNRENSLTRGLKKLTRTWSEEKNLAAAHASREEEMYRPSPVGVPLEFLVPAALGLHERSRSVQDLPDAERDSSFMRKLSLRFKKTPPTERKQKPAEEVEGTGRRLSWSIGRGSSKDKKDTESLNSETGSIETITDKHLKDQKKSSQSPVLAMRKKIGSTMDRLSMKLRSQSEDRRDAERTEKKEGRTDKSSPLMSLLRRSNSEGENLKKLGIPQNQLASQVSAPSTESIESGLSIKSEMVVKDDRRSRWDRWGLSRSKKEKMTSQPSIPASLMHEDGSIVGRQYIRNESDFPPVFHIKLKDIVVLEGEPVTLSCLPAGSPSPQILWKKDKMSLERGEKVHIKSCPDGRQQLIIPAAGQREAGLYECVAANALGSVASSCTVSVARIPDPPGTPEIPQKYKNTVLVLWKTHEHNSPCTYILEKQVNGDGEWKVLSSGITDCYFNVTELQEGTVRFRVACANKAGKGPYSNVSKTIVVDAEDRKPGTPSVQKARPVGAAVSTTVPMTLRTPSTPAVAPPVVSTPKLPPSCGSALAVSSTVITTTATVSPAQPLPKGPPPATPPRKHRGILSTPPTMHKPVIATTLSADITPQTLPNNRAPSSVAPPAVCSHVSTVTVSMSPGSSVTHLRAPFSTPMQKPTPEPITKPLPVSQVQSPKAPSVPTEQTQKIALAPAVAQVKAPFVPTTAKTNEPSRLPYSVPTEPQMPSQLAAPKLSAKSPSVPIMLHIPPFKSATLPASSPTSKTISFPSPPTSPVTKPLTPTSPAYMVTSFISMPVPSPSVESSTPPTPLTPITPPTPITPPTPLTPTTPPTPISPTSPTAPFTPTSQTVPTRVIVRSVTPGKDARYTPGGRVTPSGRESTTLRQGVPQKPYTFLDEKARGRFGVIRECKENATGKHFMAKIIQYEPENKQNVLQEYEILKGLHHQRILSLHEAYITPRYLVLISENCTGRELLYCLVERFRYSEDDVVNYLLQILQGLEYLHEQKVLHLDIKPENVIVSYMNTVKIIDLGSAQNFTPLVLRPLGKRIGTLEYMSPEMLKGDAVGPAADIWGVGVLTYIMLSGRSPFFELDPVETENKILSGRFDIFKLYSNASQSASLFIRKILTIYPWSRPSLKECFSNPWLQDAYLMKLRRQTLTFTTNKLKEFLVEQQRRRADTATKHKVLLRSYHGSQPSAPVTQ

Target ORF information:

RefSeq Version	XM_031892843.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis striated muscle enriched protein kinase (speg), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031892843.1

ORF Insert Sequence:

ATGCATCGTG CCTATGGGAG GAGGAACCTG GCCCGGAATG AAGAGGAAGC CCCTGTCTCC 
CCAACTCAAA CGTTTAGGTC TCCACCAAGC CCAGGCATTC CCCCAAAACA CCCCAAGTTG 
GCGGCTGAAC CTGGGCAGCC CTCTCCCCCT CAGTTTGTCC GGAAGCTGAA AAATGCTGCT 
ATAGGCACAG GCTGTGACAT CTGCCTAAAG GTGGCAGTGG CAGGGCACCC GCTGCCAACC 
CTAAACTGGT ACAAGAACCA GGAACGCATC CAACCCGGAG GAGAGGAATA TGGGACACTA 
TGCATCAGGG ACAGCAGGAT GGAAGATGCT GGAGTGTACA CCTGCGTGGC CCAGAATCCC 
CTGGGGGAGG CTATTACCTC TGCTGTGCTG GCAATCATTG AATTAGAAGA CTCAGAACCA 
GGGGAAGAAG ATGCAGGGGG CCCCCAATTA CAACGCACAA TTCGGGCACC CAACAATACG 
CCTCTGGGAA CCCCTTCAGG GGATTCAGAT ACGCTGCTGG CGAGCTCCCT GCACACCACG 
CCCAGCACGC TGACTGCACT CTCTCAAACA GACGAGCTCT CCGATTGGTC AGGCAGCCAA 
CAGACAGTAC TAGAAAGGGA CCCAAGGAAT GCAAGGCAAG GCTTCACTCA GGGAGGAGGG 
GCTCGCCATC TGGGGGTGGA GCCCCTCATT CCAGCTTCCA GCAACGAGTT CCCGGGCAGT 
GTCTGGGGGT CAGAGGTCAG CCTTTCTGGG TTCAGTGATC TGTATAGCAG CGCCTTCAGC 
CTGTACCGAG GCAGATCTTG TGCCATCCGG GTGTTCCTTC CGGAAGGGGG TCTGCGGAGG 
GGTAGCATCA ATGGTTCTGT AACAGACTCC CCACGAAACT TTGTCAATCC TAGGCCGCCT 
ATCATACCAC CACCCTCTCC GCGCATTGGC CAGCGTCCAG CTGTCATTCC TGCAGGCCGT 
GGACCCCCCA CCCCTGTTAC ACCTAGAAAG AAAACCACAA TGCCCAACCA GTACCAGGAC 
ACAGTGACTG AGGAGTTAGA AGAGAAGATT AAGAAGCCAA AGTCCTCTGT TGGCTCTCAG 
GCTAGTGGCC AAGATTCTCG CCCTGTAACT CCACTAAGTG AGACATCAGG GCGGGTGTCT 
ATCCTTAGGG CATCCCCAAA GCTAGTCCGC TCAGGCTCTA AGATCTTTGA AAAGCTTAGG 
TGTCTCGAAG AGAGGAGAAA AAGTCTGGAC CAGACAGACA GCCCCTTTCC AGTGCACTCA 
TGGTTGCCTC TCCGTAAGGC ACGATCCTTT GATCAGCCGG GGGCTGAGGG GCTTGGTGCT 
GGTCTAGGTT CCTCAAACGA AGAGCTAAGG GATGATCTTA GAGACGGGGT AAGATCAGAA 
ATAGGTGGGT TTACTCTGAG GTACCCCTCT CTGAGACACA AGACTACCTC ACTGGATGAC 
AGGGGAGCTC TCTATGGACG AATAAGTGAC ATTGAGACCC GCTTCTCACA AGAGCTGACT 
CGCATCAAAA GAACTGTGTC CCAGCAACAG CTAGTCCGTT CCTCACAGGA TCTATCTGGA 
AGATCACCGT CACCCCAAAG ATCCCTAGCT GCCCAGCCTG CTCCTGTGGA CCCTAAGCTA 
ACCAAAGTGT CAACGGCTCC AAAGCCTTCT GAAACAGAAG AGAAGCAGCC AGCAAGGAAG 
CCACTAGTCA GAAGCTTTGC CACCGTCAAC CAACTGTCAG AAATTAAGGA ACCTCTGGGG 
TTTTCAAGCC AACCCCCTAC ACTGCCATCA AAATCATCCA ATGGTTCTGC AGTATATCAG 
GATGCAGTGA GGCACTCGGA AACAAATATG AAATCACAAC CACACCTTAC AGGAAAAATC 
ATTGCACCAA AAACTGTTTC CCCACAAGTA TTTAAAGCTA TGACTCTCCC CGCACAATCC 
CAAAAAGAGG CAGTCGGCGA CACACAGCCG AAGAGAGTTC CTGATGTTGG TAGCGTGCCT 
CCGCCACCCA GCACACATGC CCCGGCAGCC CTAGAGATAG CTCAGAGGGG ACTTGTTAAA 
GAGAAGGGGA CTGTAAGTGA AGAGAAGAAG TATCTACCCT GGGCACTTCC TGTAGGAGAC 
AATAACAACA GCCAAATGCC ACAGGGTAAG GGTGGGGGAC AGGCCAAAAA GCACAGCGAT 
GCACACACCA GCAGCAAAAG CAACAAAAGC GGCAGAAGCA AAGGGAAGAG TCGCCGAAGC 
AGGCCATTGT CACCAGAACT TGAGTCATCG GATGATTCCT ACGTATCGGC GGGAGAGGAT 
CCAGCAGAGG CGCCTGTGTT TGAGATTCCT TTGCAAAGTG CACTAGTAAA CGCCGGCACT 
GAGGTCTTAC TGAAATGTAT TATATCTGGA AACCCAAGTC CAGAAGTGTT TTGGAGAAAG 
GATCACGTGT TGCTGAAAAA CAGTCCGACC CATCAAATAC GAGTAGAGGG CGAGAGACAC 
ACGTTGCTAC TGCGCTGGGC ACTGCCCTCA GACTCGGGTA TCTATACAGT GACTGCGCGG 
AATGAAGTTG GGGAAGCCAG CAGCTGCGGT GCCCTCACTG TAAAACCAGC ACCTACCAAA 
GAGTCGCCGG CACATCGGGG AACACCTCGT GACCTGCTCA GCCCAATAAC ATCGGATGAA 
GAGTATCTAA GCCCCCAGGA AGATTTAAGT GAGCCTACCA CCCCCCAACA TAAGATGGCA 
GGCAAAGGCC CCGCATTCAA AGCTCCACCC ACTTTCAAGA TGCCTCTTTT GGACCAGAAA 
GGGTTTGAAG GCCAAGAGAT TGTTCTCAGC GTTCAGGTGG AAGGGGAACC CAAGCCTATT 
ATCAACTGGC TGAAGAACAA GCAGCAGGTG AAGCCTGGTG GAAGGTTCCG CATATCAGAA 
GGTGCTTGGG GGATCTTCTC GCTACATATT GCCGGGGCTG ATAAACGAGA TTCCGGATTC 
TACACCTGTA AAGCAATAAA TGAGTATGGG ACAAAGCAAT GTGAAGCCAA GATAGAGGTT 
CTAGCACCCC CTGGCAGTAC CAGCTTGGAG GTTCTGGCCC CCTTGCAGGA TGTGAGTGTG 
TGCGCTGGGG AGGCAGCGGT GTTTGAATGC GTGGTGTCGG GCCCCGCCGA TATGGACGTG 
GACTGGATGT TCCGGGGCAA GCTGCTCCAG CCAGCACTGC TGGACTGTAA GATGCGTTTC 
AACGGAAAGC AGTGTGTGCT GCTGCTGAAT TCAGTCCATG AAGATGACAG TGGGGTCTAC 
ACCTGCAAAC TGAGCACTGC AAGAGATGAG CTGACCTGTA GCGCTATGCT AACTGTGCAC 
CCATCGCTGG CGCCTCTCTT CACTCGCAAG TTGTCAGATC GAGATGTTAT AGAGGGACGC 
ACAGCTCGGC TGGACTGTAA AATCAGTGGC ACCCCGCCCC CTGTTGTCAC GTGGACTCAT 
TTTGGACAGC CCATAGAGGA AAGTGAGAAC ATCCACGTTT TGAGAGAAGG TGGACATCAC 
ACCTTACTGA TACCCCACGT GGCGAATGAG GACGAGGGTC AGTACACAGC GACTGCCCAT 
AACAAGCATG GGGAGGCCGA ATGCTCTGCT GAACTCTACG TGGAGGAGCC TCGTTCTGCT 
ACAGCATCTC ATATCTCCAA GCTGGAGAAG ATGCCATCTA TTCCAGAAGA GCCAGAGATG 
CAGGAGTGTG AGGTAGAGGG TGTGATGATG CCGGATTTCC TGCGACCTCT GCAGGACATT 
GACGTGGTGG AATTTCGGGA GGTACAACTT GAGTGTCAGG TAACCGGTCT GCCGTACCCT 
ACCATCACCT GGTATCACAA TGGTCAGAAG ATCCAGAGCT CTGATGATCG CAGAATGACT 
CAATATAAAG ACATCCACCG CCTGGTTTTC CCCTCTGTTA CTCATTCTCA TGCCGGAGTC 
TATAAAAGTG TCATCTCAAA CAAAGTGGGG AAAGCAGCCT GCTATGCGCA CCTGTATGTC 
ACAGATTTGG TACCCACTCC TCCAGATGGT CCCCCCATCA TTACCTCTGT AACTGGGAGA 
ATTGTGAAGC TCCAGTGGAA GCCACCCAAG AGATTGGACC CAGCCATTGA TCTGGCTCAG 
ATTACATACT CAGTCCAGCA GCAAGCGGTG GGATCTCCCC AATGGACAGT CATCACCACC 
AACCTAAAAG ACACTTGTTA TTGTGTCAGA TCTCTGACCA AGGAACATCA GTATCTTTTC 
CGTGTGACCA CAAACACCCC AACTTCTCAC AGTAAACCTT CCCCTCCCAG TGAAAGCGTT 
TGTCTCTTTA ATAGAGGTCC CTACCTGGAG GAGATGCCTT CTATACTGGA TCGGCCTGAA 
ATCTTGTATA TGGTGGAAAA TCAGCCTCTT TGCATAACCG TGACACTGAA CCACGTACAT 
GCCAAAGTCA CGTGGCAGAG AATAGACACC GTTCTGAGCA ATGTCGAGGG TGTGTATGAG 
CTGAGTATGC CGGATGACGA CCAGCACTCG CTCACGTTGT TCAGCCCAAG GAAGTCTGAC 
CTGGGGCAGC TGACATTTGA GGCTAAAAAC CGCTACGGAT CAGATAGCTG CACTATTTCC 
ATCGAGCTCG CAGAGGCTCC TCGCTTTGAG TCCATCATGG AAGACATAGA GATCGGTGTT 
GGTGAAACAG CTCGTTTTGT GGTCGTGGTG GAAGGGAAGC CTATTCCAGA CATCATGTGG 
TACAAAGATG GGGTACTTTT GGCAGAGAGC GGTCACTTCA GCTTTGTGTA TGATGATACC 
GAGTGCTCTC TGGTGATCTT AAACACGGCA CTTGGTGACT CCGGTGTGTA CACCTGCACG 
GCACGGAACC TTTCAGGGGA AATTTCCTGC AAAGCGGAGC TGCAAGTGTG CCCAGATGAA 
CCAGGGGCAG GATCTGCAAT ACAGGAAATA GATATACTCA AAGTTCGTAG ATTGACTGAT 
TTCTACAACA TCCATAAAGA AATTGGCAGA GGGGCCTTCT CCTATGTCCG ACATGTGGTA 
GAAAAGAGCA ACGGGCGAGA TCTTGCTGCT AAATTCATAC CGTTCCGTGG GGCAACCAGG 
GAGAGTGCAC GGAGGGAGCG GGACATACTG TCCAAGCTCA GACACGACAG GATCATCTTC 
TTCTACGATG CATTTGAGAA AAGAAGTGCT CTAATCATTG TCATGGAACT CTGTTCTCAA 
GAGGAGCTCC TAGAGAGGTT GACAAGAAAG CCTACAGTGA GCGAATCAGA GATCCGCTCG 
TTTATACGGC AAATCCTAGA AGGGTTAGAT TATTTGCACC ATAAAAACAT TCTGCATTTG 
GACATAAAGC CAGAGAACAT CCTGATGGCC GATACGACTA GTGAACAGAT TCGCATTTGT 
GACTTTGGAA ACGCTCAGGA ACTAACCCCG GGGGATCCTC AGTACTGCAA ATACGGAACC 
CCGGAATTTG TGGCGCCCGA AATTGTGAAC CAGATGCCAA TTTCTACAGT AACGGACATA 
TGGCCAGTTG GAGTCCTGGC ATATTTATGC CTTACCGGAG TGTCGCCCTT TGTTGGGGAG 
AATGATTACA CCACTCTGAT GAATATCCGG GGTTACACTG TGGCATTTGA AGAGAAGATG 
TTTGTTGGTC TCACACGAGA AGCACGTGGT TTCCTAATCA AAGTCTTGGG CAATGAGAAG 
CTGAGGCCTA ATGCTGAAGA GAGTTTGGAG CACCCCTGGT TCAAAACTTT AGCGAAAGGG 
AAAAGCATCA GCACAGACCA TCTGAAGCTG TTCCAGTCTC GCCGTAAATG GCAGCGCTCA 
CTTATCAGCT ATAAGTCCAA TATGGTGATG AGAACCATTC CAGAGCTGCT TCAGGACACA 
AGCAATCACT TGTCCATTGC TGTACCCAAG AATTCCAAGG AAGCATCCGG ATTGTCATCC 
TCCTCTGATT CTGATGACCT GGAAGAGCTT CCCTTTGTCC CTATGCCTCT CCAGGTCCAA 
TTCTCTGGAT CACGCATGTC CTTGAATGAG ATCCCAACAG ATGAGGAACA ACTGTCTCAG 
CCCCAGGACA ATGGCAGCTG TAGAGAAGAT GAGGAAGAAG GAAGTCAGGC AGTGGAAATT 
AGTAGCACAT TTAAAGAACA AGCAGTTCAG CAGAAAGAAG CAACAGTCCA AGCAAGTCCA 
TTGTCCAAGC ATGCCAAAGG AACCCTTACA AGGAACAGGT CTGCTGAAGT TGGTTCTTCT 
TCTGATGAGG AAACTTCAGA AACTGAAAAA AGAGAGCATC CTCGTAGGCC TCTGAAAAAA 
GGTTCCAGCT TAGAATCTGC CGACGTTCCT GATGGGGAAA TTAAAGCAGC TCGTAGAGGA 
GAGCTGAGGA GGGGAAGCTC TGCAGATAGT GCTCTTTTGC TCAATGTGTC TACAACAGAT 
GGAGATGAAG AGAGAGATCG ACCAGACAAG GGAATGATAA AAGCTGCCTC AATGGAGCTA 
CCAACCAGAT GCAGGAGCCC AGGTAGATTG GAGGATCGAG GCACACTACG AAGAAAGTTT 
GGATCAGCTG ATGAAGAATA TGCTCAGCGG TTAGAAATGA TGAGACAGAG GCTATTGAGG 
GGAGGTTCGG CAGATGGAAA ACTCAGTGGT CTTAGAGGCC CACTCATAGA AACACTTTCT 
GTAGACAAGA AGCGAGCAGA GCAGATATCT ATACGCTCCC CAAGAAGTGA TCAGTCTTCT 
CTGAGTCCTA TTCCCGCCAT CAAACTAACC CGAGCTGCTT CCAGTGAGGC TGCGCCTGGT 
AGAGAAGCAA CTGAAGAAAG AGTCTTAAGG AAAACCAGTT CCTTTTCTCA CAGCGACACA 
GAGCCACTGG TCTTGCATCG CAGGTCAGGG GCACCCCTAG AAATACCTCT TGCTCAGTTA 
GAGGCACAGC GTCTTAAAGA GTCTCCCTCT CTTTCAGCAT TGACAGACCG ATTAGAATCA 
CGTCCTCAAA CACCGCGAGA GATGATTCCC AAATCTCTAA CTCCAGAAGC ACCGCAATTG 
AAGGCAGAAA GTGGGGAGCC AGAAACTCAG AGTGATAAGG AAGCTGAGAA GGGCACCACA 
CCTCTTATTC AGCATCATAA AGACAAAGAC TCAAATTCAT TGAAGGACTT GGTTATTGTT 
CTTCCAAGAG TGACTCCACC TTTGGATAAA ATGGCATCTA AAACAGAGTT ACCAAGTATT 
CAAACAGAGA GGGCACAGGA CAAAATCCCT AGAGCAGATG TGAACCTGCC AGCTACTACT 
TCATCAAATG AAACTGTGCC AACAAAGCCA CCACCCTTAC TTTTGCCTTC TAAGCAGTCT 
CTTTCTGTGC CACCTGAACC TTTACCCTCT TCAGTACTGA CAAATGAGAT GCCTAAATCT 
AAAGAGGAGT ACTCGTCATC AAGGGCCATT AAGGCCAAGG AATCCATATC AAAAACAGAA 
ACAACAGACC AGCAAGATAA AATTAAAGAA CCTTTACCAC AAGCTGTTGT TCCATTACCA 
TTGCCTATGC CAGCCTCCCA AGTGCTAAAA CCTTCCACTG CAAAAGTACA GATAGCAGAT 
TCTCCAACAA TGCCTGAGAA TGATGCATCA CTTCCACAGA TAACAGGTAA AGAGAAACTA 
TCTTCTCCAG CTGTCCAACC AGTAGATAAA ACAGCAGCTA TATCATCACC TTATGCTGCT 
ATGATTCAAA CACTTCAAGT GCCCGAGGAA CCTGAGACAG TTGTTGAGGA ATGTTCAAAG 
GCCCCATTAA AAGCTCAGCC AGTATTTCAA GAAATTCCTA TAGCCACTGG AGATGCAAAG 
GCTCAAGGAA TATCACAAGT AAACAATAAA GAAGGCAAGG ATGAGCTATC ACAAGAACCC 
AAGCTGATTA ACACCAGATC TTCCAGCACA GAGAATGTCC CATTTATCAG CAACATTGAT 
TCAGAAGAAA TCTTTGAGGC TAAGTTCAAG AGGAACCGTG AAAATTCTCT TACCCGAGGT 
CTAAAAAAGC TTACCAGGAC TTGGTCAGAG GAGAAGAATT TAGCTGCAGC CCATGCATCT 
CGGGAAGAAG AAATGTACAG GCCTAGTCCT GTGGGTGTCC CACTAGAGTT CCTTGTTCCT 
GCAGCTCTTG GGCTACATGA AAGATCAAGA TCAGTGCAAG ATCTGCCAGA TGCTGAGAGG 
GATTCAAGCT TTATGAGAAA GCTTTCTTTG AGATTTAAGA AAACTCCACC AACTGAAAGG 
AAGCAGAAAC CAGCAGAAGA AGTAGAAGGT ACTGGTAGAC GACTTTCATG GTCAATAGGT 
AGAGGAAGTT CCAAAGATAA GAAGGATACA GAGAGCCTGA ACTCTGAAAC AGGCTCAATA 
GAAACCATCA CAGACAAACA TCTAAAGGAC CAGAAAAAGA GTAGTCAATC ACCAGTGTTG 
GCCATGAGAA AGAAAATAGG CAGTACTATG GATCGACTTT CTATGAAACT AAGGAGCCAA 
TCAGAAGATA GAAGGGATGC AGAGAGAACA GAGAAGAAAG AAGGGCGTAC AGACAAAAGT 
TCTCCATTAA TGTCTTTACT TAGGAGATCC AACTCAGAAG GAGAGAACTT AAAGAAACTA 
GGTATTCCCC AGAACCAACT TGCTTCCCAG GTTTCAGCTC CATCAACTGA GTCTATTGAA 
TCTGGTCTCA GCATCAAATC TGAAATGGTA GTCAAGGATG ATAGGCGCTC AAGGTGGGAT 
CGTTGGGGCC TGTCACGTAG CAAAAAGGAG AAGATGACAT CGCAGCCAAG TATTCCAGCC 
AGTCTGATGC ATGAAGATGG CAGTATTGTT GGCAGACAAT ACATTCGTAA TGAGTCAGAC 
TTCCCCCCAG TCTTCCATAT CAAGCTGAAG GACATCGTGG TGCTAGAGGG AGAGCCGGTG 
ACTCTGTCCT GCTTACCTGC TGGCAGCCCT TCCCCCCAGA TCCTGTGGAA GAAAGATAAG 
ATGTCCTTGG AAAGAGGAGA AAAGGTGCAT ATAAAATCCT GCCCCGATGG CAGACAGCAG 
CTCATTATTC CCGCCGCCGG GCAGAGGGAA GCCGGTCTGT ATGAATGCGT AGCAGCCAAT 
GCCCTGGGCT CAGTGGCCAG CTCCTGCACA GTGTCTGTGG CAAGAATACC TGATCCCCCG 
GGGACCCCAG AGATCCCACA GAAGTATAAG AACACGGTGC TGGTTCTATG GAAGACCCAC 
GAGCACAACT CCCCTTGCAC CTACATCCTG GAGAAACAGG TTAATGGTGA TGGGGAATGG 
AAGGTTCTAA GTTCTGGAAT CACCGACTGT TACTTCAATG TGACGGAGCT CCAGGAAGGC 
ACTGTCAGGT TCCGCGTCGC TTGTGCCAAC AAAGCAGGAA AGGGGCCCTA CAGCAATGTG 
TCCAAGACAA TTGTCGTAGA TGCAGAAGAT CGTAAGCCAG GAACACCCTC TGTCCAGAAG 
GCACGGCCCG TGGGAGCTGC AGTGTCCACT ACTGTTCCAA TGACTCTCCG GACCCCTTCT 
ACCCCAGCTG TTGCGCCCCC TGTTGTCTCT ACCCCCAAAT TACCTCCATC TTGTGGATCT 
GCTCTTGCAG TTTCCTCAAC AGTTATCACT ACAACCGCAA CTGTGTCTCC AGCCCAGCCT 
CTCCCCAAAG GGCCTCCACC TGCAACTCCT CCACGGAAAC ACCGAGGGAT ACTCTCCACT 
CCCCCAACCA TGCACAAGCC TGTCATTGCC ACCACTCTCA GTGCCGATAT TACTCCCCAA 
ACTCTGCCCA ATAACAGGGC TCCATCTTCT GTGGCTCCTC CTGCCGTATG TTCTCATGTC 
TCCACGGTGA CTGTGTCTAT GTCACCAGGT TCTTCGGTGA CCCATTTAAG GGCCCCCTTC 
TCTACCCCCA TGCAGAAACC TACCCCAGAA CCAATAACAA AACCATTACC AGTGTCACAA 
GTACAATCAC CCAAAGCTCC TTCTGTGCCA ACAGAGCAAA CCCAGAAAAT TGCCCTTGCC 
CCGGCTGTTG CCCAGGTTAA AGCACCTTTT GTGCCAACTA CTGCAAAAAC TAACGAACCA 
TCACGTCTTC CATATAGTGT CCCTACGGAA CCACAGATGC CATCACAGTT AGCAGCTCCA 
AAACTATCTG CCAAGTCCCC CTCTGTTCCA ATTATGCTGC ACATTCCCCC CTTTAAATCA 
GCAACCCTAC CTGCTTCCAG TCCCACTTCC AAAACCATAT CTTTTCCCTC TCCTCCTACA 
AGCCCAGTAA CCAAACCCTT AACACCTACC TCCCCAGCTT ATATGGTCAC TTCCTTTATA 
TCAATGCCTG TGCCTTCACC CTCTGTTGAG TCCTCCACTC CACCTACTCC ACTTACCCCC 
ATCACCCCAC CTACCCCCAT CACCCCACCT ACCCCACTTA CCCCCACCAC CCCTCCTACC 
CCCATTAGCC CCACTTCCCC AACCGCCCCA TTTACCCCCA CTTCCCAAAC TGTCCCTACA 
CGGGTGATAG TAAGAAGTGT CACCCCCGGG AAGGATGCAC GTTATACACC CGGAGGAAGA 
GTCACACCCT CGGGGAGAGA AAGCACCACC CTCCGACAAG GGGTACCCCA AAAACCTTAC 
ACTTTCTTGG ATGAGAAAGC AAGGGGGCGG TTTGGAGTGA TCCGAGAGTG TAAGGAGAAT 
GCAACTGGGA AGCACTTCAT GGCAAAGATA ATCCAGTACG AACCAGAAAA CAAGCAAAAC 
GTGCTGCAGG AGTATGAGAT CCTCAAGGGT CTCCACCATC AACGAATCTT GAGTCTTCAT 
GAAGCTTATA TTACCCCTCG CTACCTTGTG TTGATTTCTG AAAACTGCAC AGGACGGGAA 
CTGCTTTACT GCCTAGTTGA GAGGTTCCGT TATTCTGAAG ACGACGTGGT CAATTACCTC 
CTGCAGATCC TACAGGGGTT GGAGTACCTC CATGAACAAA AGGTCCTGCA CCTCGATATC 
AAACCTGAGA ACGTCATTGT GTCCTACATG AACACTGTCA AGATCATCGA TTTGGGGAGT 
GCCCAGAACT TTACACCGCT GGTCCTGCGA CCCCTCGGCA AACGCATAGG GACCCTGGAA 
TATATGTCCC CCGAAATGCT CAAAGGTGAC GCAGTAGGCC CAGCAGCTGA TATTTGGGGT 
GTAGGCGTTC TGACTTACAT AATGCTCAGC GGCCGCTCTC CGTTCTTTGA GCTGGACCCC 
GTGGAAACCG AGAACAAGAT CTTGTCGGGA CGATTTGATA TCTTTAAATT GTATTCCAAC 
GCCTCCCAAA GTGCTTCCCT CTTCATTCGC AAGATCCTGA CTATATATCC TTGGAGCCGC 
CCATCACTTA AGGAATGTTT TTCCAATCCC TGGCTCCAGG ATGCCTACCT AATGAAGCTT 
AGACGCCAAA CCCTAACCTT CACCACTAAC AAGCTCAAAG AGTTCCTCGT GGAGCAACAG 
AGGAGACGCG CAGACACAGC CACTAAGCAC AAAGTCCTCC TTCGTTCGTA CCACGGAAGC 
CAGCCCTCAG CTCCTGTCAC TCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

speg ( XM_031892844.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031748704.1 Xenopus tropicalis striated muscle enriched protein kinase (speg), transcript variant X2, mRNA.

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CloneID	OXa66223
Clone ID Related Accession (Same CDS sequence)	XM_031892844.1
Accession Version	XM_031892844.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	striated muscle preferentially expressed protein kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCGTG CCTATGGGAG GAGGAACCTG GCCCGGAATG AAGAGGAAGC CCCTGTCTCC 
CCAACTCAAA CGTTTAGGTC TCCACCAAGC CCAGGCATTC CCCCAAAACA CCCCAAGTTG 
GCGGCTGAAC CTGGGCAGCC CTCTCCCCCT CAGTTTGTCC GGAAGCTGAA AAATGCTGCT 
ATAGGCACAG GCTGTGACAT CTGCCTAAAG GTGGCAGTGG CAGGGCACCC GCTGCCAACC 
CTAAACTGGT ACAAGAACCA GGAACGCATC CAACCCGGAG GAGAGGAATA TGGGACACTA 
TGCATCAGGG ACAGCAGGAT GGAAGATGCT GGAGTGTACA CCTGCGTGGC CCAGAATCCC 
CTGGGGGAGG CTATTACCTC TGCTGTGCTG GCAATCATTG AATTAGAAGA CTCAGAACCA 
GGGGAAGAAG ATGCAGGGGG CCCCCAATTA CAACGCACAA TTCGGGCACC CAACAATACG 
CCTCTGGGAA CCCCTTCAGG GGATTCAGAT ACGCTGCTGG CGAGCTCCCT GCACACCACG 
CCCAGCACGC TGACTGCACT CTCTCAAACA GACGAGCTCT CCGATTGGTC AGGCAGCCAA 
CAGACAGTAC TAGAAAGGGA CCCAAGGAAT GCAAGGTTCC TTCCGGAAGG GGGTCTGCGG 
AGGGGTAGCA TCAATGGTTC TGTAACAGAC TCCCCACGAA ACTTTGTCAA TCCTAGGCCG 
CCTATCATAC CACCACCCTC TCCGCGCATT GGCCAGCGTC CAGCTGTCAT TCCTGCAGGC 
CGTGGACCCC CCACCCCTGT TACACCTAGA AAGAAAACCA CAATGCCCAA CCAGTACCAG 
GACACAGTGA CTGAGGAGTT AGAAGAGAAG ATTAAGAAGC CAAAGTCCTC TGTTGGCTCT 
CAGGCTAGTG GCCAAGATTC TCGCCCTGTA ACTCCACTAA GTGAGACATC AGGGCGGGTG 
TCTATCCTTA GGGCATCCCC AAAGCTAGTC CGCTCAGGCT CTAAGATCTT TGAAAAGCTT 
AGGTGTCTCG AAGAGAGGAG AAAAAGTCTG GACCAGACAG ACAGCCCCTT TCCAGTGCAC 
TCATGGTTGC CTCTCCGTAA GGCACGATCC TTTGATCAGC CGGGGGCTGA GGGGCTTGGT 
GCTGGTCTAG GTTCCTCAAA CGAAGAGCTA AGGGATGATC TTAGAGACGG GGTAAGATCA 
GAAATAGGTG GGTTTACTCT GAGGTACCCC TCTCTGAGAC ACAAGACTAC CTCACTGGAT 
GACAGGGGAG CTCTCTATGG ACGAATAAGT GACATTGAGA CCCGCTTCTC ACAAGAGCTG 
ACTCGCATCA AAAGAACTGT GTCCCAGCAA CAGCTAGTCC GTTCCTCACA GGATCTATCT 
GGAAGATCAC CGTCACCCCA AAGATCCCTA GCTGCCCAGC CTGCTCCTGT GGACCCTAAG 
CTAACCAAAG TGTCAACGGC TCCAAAGCCT TCTGAAACAG AAGAGAAGCA GCCAGCAAGG 
AAGCCACTAG TCAGAAGCTT TGCCACCGTC AACCAACTGT CAGAAATTAA GGAACCTCTG 
GGGTTTTCAA GCCAACCCCC TACACTGCCA TCAAAATCAT CCAATGGTTC TGCAGTATAT 
CAGGATGCAG TGAGGCACTC GGAAACAAAT ATGAAATCAC AACCACACCT TACAGGAAAA 
ATCATTGCAC CAAAAACTGT TTCCCCACAA GTATTTAAAG CTATGACTCT CCCCGCACAA 
TCCCAAAAAG AGGCAGTCGG CGACACACAG CCGAAGAGAG TTCCTGATGT TGGTAGCGTG 
CCTCCGCCAC CCAGCACACA TGCCCCGGCA GCCCTAGAGA TAGCTCAGAG GGGACTTGTT 
AAAGAGAAGG GGACTGTAAG TGAAGAGAAG AAGTATCTAC CCTGGGCACT TCCTGTAGGA 
GACAATAACA ACAGCCAAAT GCCACAGGGT AAGGGTGGGG GACAGGCCAA AAAGCACAGC 
GATGCACACA CCAGCAGCAA AAGCAACAAA AGCGGCAGAA GCAAAGGGAA GAGTCGCCGA 
AGCAGGCCAT TGTCACCAGA ACTTGAGTCA TCGGATGATT CCTACGTATC GGCGGGAGAG 
GATCCAGCAG AGGCGCCTGT GTTTGAGATT CCTTTGCAAA GTGCACTAGT AAACGCCGGC 
ACTGAGGTCT TACTGAAATG TATTATATCT GGAAACCCAA GTCCAGAAGT GTTTTGGAGA 
AAGGATCACG TGTTGCTGAA AAACAGTCCG ACCCATCAAA TACGAGTAGA GGGCGAGAGA 
CACACGTTGC TACTGCGCTG GGCACTGCCC TCAGACTCGG GTATCTATAC AGTGACTGCG 
CGGAATGAAG TTGGGGAAGC CAGCAGCTGC GGTGCCCTCA CTGTAAAACC AGCACCTACC 
AAAGAGTCGC CGGCACATCG GGGAACACCT CGTGACCTGC TCAGCCCAAT AACATCGGAT 
GAAGAGTATC TAAGCCCCCA GGAAGATTTA AGTGAGCCTA CCACCCCCCA ACATAAGATG 
GCAGGCAAAG GCCCCGCATT CAAAGCTCCA CCCACTTTCA AGATGCCTCT TTTGGACCAG 
AAAGGGTTTG AAGGCCAAGA GATTGTTCTC AGCGTTCAGG TGGAAGGGGA ACCCAAGCCT 
ATTATCAACT GGCTGAAGAA CAAGCAGCAG GTGAAGCCTG GTGGAAGGTT CCGCATATCA 
GAAGGTGCTT GGGGGATCTT CTCGCTACAT ATTGCCGGGG CTGATAAACG AGATTCCGGA 
TTCTACACCT GTAAAGCAAT AAATGAGTAT GGGACAAAGC AATGTGAAGC CAAGATAGAG 
GTTCTAGCAC CCCCTGGCAG TACCAGCTTG GAGGTTCTGG CCCCCTTGCA GGATGTGAGT 
GTGTGCGCTG GGGAGGCAGC GGTGTTTGAA TGCGTGGTGT CGGGCCCCGC CGATATGGAC 
GTGGACTGGA TGTTCCGGGG CAAGCTGCTC CAGCCAGCAC TGCTGGACTG TAAGATGCGT 
TTCAACGGAA AGCAGTGTGT GCTGCTGCTG AATTCAGTCC ATGAAGATGA CAGTGGGGTC 
TACACCTGCA AACTGAGCAC TGCAAGAGAT GAGCTGACCT GTAGCGCTAT GCTAACTGTG 
CACCCATCGC TGGCGCCTCT CTTCACTCGC AAGTTGTCAG ATCGAGATGT TATAGAGGGA 
CGCACAGCTC GGCTGGACTG TAAAATCAGT GGCACCCCGC CCCCTGTTGT CACGTGGACT 
CATTTTGGAC AGCCCATAGA GGAAAGTGAG AACATCCACG TTTTGAGAGA AGGTGGACAT 
CACACCTTAC TGATACCCCA CGTGGCGAAT GAGGACGAGG GTCAGTACAC AGCGACTGCC 
CATAACAAGC ATGGGGAGGC CGAATGCTCT GCTGAACTCT ACGTGGAGGA GCCTCGTTCT 
GCTACAGCAT CTCATATCTC CAAGCTGGAG AAGATGCCAT CTATTCCAGA AGAGCCAGAG 
ATGCAGGAGT GTGAGGTAGA GGGTGTGATG ATGCCGGATT TCCTGCGACC TCTGCAGGAC 
ATTGACGTGG TGGAATTTCG GGAGGTACAA CTTGAGTGTC AGGTAACCGG TCTGCCGTAC 
CCTACCATCA CCTGGTATCA CAATGGTCAG AAGATCCAGA GCTCTGATGA TCGCAGAATG 
ACTCAATATA AAGACATCCA CCGCCTGGTT TTCCCCTCTG TTACTCATTC TCATGCCGGA 
GTCTATAAAA GTGTCATCTC AAACAAAGTG GGGAAAGCAG CCTGCTATGC GCACCTGTAT 
GTCACAGATT TGGTACCCAC TCCTCCAGAT GGTCCCCCCA TCATTACCTC TGTAACTGGG 
AGAATTGTGA AGCTCCAGTG GAAGCCACCC AAGAGATTGG ACCCAGCCAT TGATCTGGCT 
CAGATTACAT ACTCAGTCCA GCAGCAAGCG GTGGGATCTC CCCAATGGAC AGTCATCACC 
ACCAACCTAA AAGACACTTG TTATTGTGTC AGATCTCTGA CCAAGGAACA TCAGTATCTT 
TTCCGTGTGA CCACAAACAC CCCAACTTCT CACAGTAAAC CTTCCCCTCC CAGTGAAAGC 
GTTTGTCTCT TTAATAGAGG TCCCTACCTG GAGGAGATGC CTTCTATACT GGATCGGCCT 
GAAATCTTGT ATATGGTGGA AAATCAGCCT CTTTGCATAA CCGTGACACT GAACCACGTA 
CATGCCAAAG TCACGTGGCA GAGAATAGAC ACCGTTCTGA GCAATGTCGA GGGTGTGTAT 
GAGCTGAGTA TGCCGGATGA CGACCAGCAC TCGCTCACGT TGTTCAGCCC AAGGAAGTCT 
GACCTGGGGC AGCTGACATT TGAGGCTAAA AACCGCTACG GATCAGATAG CTGCACTATT 
TCCATCGAGC TCGCAGAGGC TCCTCGCTTT GAGTCCATCA TGGAAGACAT AGAGATCGGT 
GTTGGTGAAA CAGCTCGTTT TGTGGTCGTG GTGGAAGGGA AGCCTATTCC AGACATCATG 
TGGTACAAAG ATGGGGTACT TTTGGCAGAG AGCGGTCACT TCAGCTTTGT GTATGATGAT 
ACCGAGTGCT CTCTGGTGAT CTTAAACACG GCACTTGGTG ACTCCGGTGT GTACACCTGC 
ACGGCACGGA ACCTTTCAGG GGAAATTTCC TGCAAAGCGG AGCTGCAAGT GTGCCCAGAT 
GAACCAGGGG CAGGATCTGC AATACAGGAA ATAGATATAC TCAAAGTTCG TAGATTGACT 
GATTTCTACA ACATCCATAA AGAAATTGGC AGAGGGGCCT TCTCCTATGT CCGACATGTG 
GTAGAAAAGA GCAACGGGCG AGATCTTGCT GCTAAATTCA TACCGTTCCG TGGGGCAACC 
AGGGAGAGTG CACGGAGGGA GCGGGACATA CTGTCCAAGC TCAGACACGA CAGGATCATC 
TTCTTCTACG ATGCATTTGA GAAAAGAAGT GCTCTAATCA TTGTCATGGA ACTCTGTTCT 
CAAGAGGAGC TCCTAGAGAG GTTGACAAGA AAGCCTACAG TGAGCGAATC AGAGATCCGC 
TCGTTTATAC GGCAAATCCT AGAAGGGTTA GATTATTTGC ACCATAAAAA CATTCTGCAT 
TTGGACATAA AGCCAGAGAA CATCCTGATG GCCGATACGA CTAGTGAACA GATTCGCATT 
TGTGACTTTG GAAACGCTCA GGAACTAACC CCGGGGGATC CTCAGTACTG CAAATACGGA 
ACCCCGGAAT TTGTGGCGCC CGAAATTGTG AACCAGATGC CAATTTCTAC AGTAACGGAC 
ATATGGCCAG TTGGAGTCCT GGCATATTTA TGCCTTACCG GAGTGTCGCC CTTTGTTGGG 
GAGAATGATT ACACCACTCT GATGAATATC CGGGGTTACA CTGTGGCATT TGAAGAGAAG 
ATGTTTGTTG GTCTCACACG AGAAGCACGT GGTTTCCTAA TCAAAGTCTT GGGCAATGAG 
AAGCTGAGGC CTAATGCTGA AGAGAGTTTG GAGCACCCCT GGTTCAAAAC TTTAGCGAAA 
GGGAAAAGCA TCAGCACAGA CCATCTGAAG CTGTTCCAGT CTCGCCGTAA ATGGCAGCGC 
TCACTTATCA GCTATAAGTC CAATATGGTG ATGAGAACCA TTCCAGAGCT GCTTCAGGAC 
ACAAGCAATC ACTTGTCCAT TGCTGTACCC AAGAATTCCA AGGAAGCATC CGGATTGTCA 
TCCTCCTCTG ATTCTGATGA CCTGGAAGAG CTTCCCTTTG TCCCTATGCC TCTCCAGGTC 
CAATTCTCTG GATCACGCAT GTCCTTGAAT GAGATCCCAA CAGATGAGGA ACAACTGTCT 
CAGCCCCAGG ACAATGGCAG CTGTAGAGAA GATGAGGAAG AAGGAAGTCA GGCAGTGGAA 
ATTAGTAGCA CATTTAAAGA ACAAGCAGTT CAGCAGAAAG AAGCAACAGT CCAAGCAAGT 
CCATTGTCCA AGCATGCCAA AGGAACCCTT ACAAGGAACA GGTCTGCTGA AGTTGGTTCT 
TCTTCTGATG AGGAAACTTC AGAAACTGAA AAAAGAGAGC ATCCTCGTAG GCCTCTGAAA 
AAAGGTTCCA GCTTAGAATC TGCCGACGTT CCTGATGGGG AAATTAAAGC AGCTCGTAGA 
GGAGAGCTGA GGAGGGGAAG CTCTGCAGAT AGTGCTCTTT TGCTCAATGT GTCTACAACA 
GATGGAGATG AAGAGAGAGA TCGACCAGAC AAGGGAATGA TAAAAGCTGC CTCAATGGAG 
CTACCAACCA GATGCAGGAG CCCAGGTAGA TTGGAGGATC GAGGCACACT ACGAAGAAAG 
TTTGGATCAG CTGATGAAGA ATATGCTCAG CGGTTAGAAA TGATGAGACA GAGGCTATTG 
AGGGGAGGTT CGGCAGATGG AAAACTCAGT GGTCTTAGAG GCCCACTCAT AGAAACACTT 
TCTGTAGACA AGAAGCGAGC AGAGCAGATA TCTATACGCT CCCCAAGAAG TGATCAGTCT 
TCTCTGAGTC CTATTCCCGC CATCAAACTA ACCCGAGCTG CTTCCAGTGA GGCTGCGCCT 
GGTAGAGAAG CAACTGAAGA AAGAGTCTTA AGGAAAACCA GTTCCTTTTC TCACAGCGAC 
ACAGAGCCAC TGGTCTTGCA TCGCAGGTCA GGGGCACCCC TAGAAATACC TCTTGCTCAG 
TTAGAGGCAC AGCGTCTTAA AGAGTCTCCC TCTCTTTCAG CATTGACAGA CCGATTAGAA 
TCACGTCCTC AAACACCGCG AGAGATGATT CCCAAATCTC TAACTCCAGA AGCACCGCAA 
TTGAAGGCAG AAAGTGGGGA GCCAGAAACT CAGAGTGATA AGGAAGCTGA GAAGGGCACC 
ACACCTCTTA TTCAGCATCA TAAAGACAAA GACTCAAATT CATTGAAGGA CTTGGTTATT 
GTTCTTCCAA GAGTGACTCC ACCTTTGGAT AAAATGGCAT CTAAAACAGA GTTACCAAGT 
ATTCAAACAG AGAGGGCACA GGACAAAATC CCTAGAGCAG ATGTGAACCT GCCAGCTACT 
ACTTCATCAA ATGAAACTGT GCCAACAAAG CCACCACCCT TACTTTTGCC TTCTAAGCAG 
TCTCTTTCTG TGCCACCTGA ACCTTTACCC TCTTCAGTAC TGACAAATGA GATGCCTAAA 
TCTAAAGAGG AGTACTCGTC ATCAAGGGCC ATTAAGGCCA AGGAATCCAT ATCAAAAACA 
GAAACAACAG ACCAGCAAGA TAAAATTAAA GAACCTTTAC CACAAGCTGT TGTTCCATTA 
CCATTGCCTA TGCCAGCCTC CCAAGTGCTA AAACCTTCCA CTGCAAAAGT ACAGATAGCA 
GATTCTCCAA CAATGCCTGA GAATGATGCA TCACTTCCAC AGATAACAGG TAAAGAGAAA 
CTATCTTCTC CAGCTGTCCA ACCAGTAGAT AAAACAGCAG CTATATCATC ACCTTATGCT 
GCTATGATTC AAACACTTCA AGTGCCCGAG GAACCTGAGA CAGTTGTTGA GGAATGTTCA 
AAGGCCCCAT TAAAAGCTCA GCCAGTATTT CAAGAAATTC CTATAGCCAC TGGAGATGCA 
AAGGCTCAAG GAATATCACA AGTAAACAAT AAAGAAGGCA AGGATGAGCT ATCACAAGAA 
CCCAAGCTGA TTAACACCAG ATCTTCCAGC ACAGAGAATG TCCCATTTAT CAGCAACATT 
GATTCAGAAG AAATCTTTGA GGCTAAGTTC AAGAGGAACC GTGAAAATTC TCTTACCCGA 
GGTCTAAAAA AGCTTACCAG GACTTGGTCA GAGGAGAAGA ATTTAGCTGC AGCCCATGCA 
TCTCGGGAAG AAGAAATGTA CAGGCCTAGT CCTGTGGGTG TCCCACTAGA GTTCCTTGTT 
CCTGCAGCTC TTGGGCTACA TGAAAGATCA AGATCAGTGC AAGATCTGCC AGATGCTGAG 
AGGGATTCAA GCTTTATGAG AAAGCTTTCT TTGAGATTTA AGAAAACTCC ACCAACTGAA 
AGGAAGCAGA AACCAGCAGA AGAAGTAGAA GGTACTGGTA GACGACTTTC ATGGTCAATA 
GGTAGAGGAA GTTCCAAAGA TAAGAAGGAT ACAGAGAGCC TGAACTCTGA AACAGGCTCA 
ATAGAAACCA TCACAGACAA ACATCTAAAG GACCAGAAAA AGAGTAGTCA ATCACCAGTG 
TTGGCCATGA GAAAGAAAAT AGGCAGTACT ATGGATCGAC TTTCTATGAA ACTAAGGAGC 
CAATCAGAAG ATAGAAGGGA TGCAGAGAGA ACAGAGAAGA AAGAAGGGCG TACAGACAAA 
AGTTCTCCAT TAATGTCTTT ACTTAGGAGA TCCAACTCAG AAGGAGAGAA CTTAAAGAAA 
CTAGGTATTC CCCAGAACCA ACTTGCTTCC CAGGTTTCAG CTCCATCAAC TGAGTCTATT 
GAATCTGGTC TCAGCATCAA ATCTGAAATG GTAGTCAAGG ATGATAGGCG CTCAAGGTGG 
GATCGTTGGG GCCTGTCACG TAGCAAAAAG GAGAAGATGA CATCGCAGCC AAGTATTCCA 
GCCAGTCTGA TGCATGAAGA TGGCAGTATT GTTGGCAGAC AATACATTCG TAATGAGTCA 
GACTTCCCCC CAGTCTTCCA TATCAAGCTG AAGGACATCG TGGTGCTAGA GGGAGAGCCG 
GTGACTCTGT CCTGCTTACC TGCTGGCAGC CCTTCCCCCC AGATCCTGTG GAAGAAAGAT 
AAGATGTCCT TGGAAAGAGG AGAAAAGGTG CATATAAAAT CCTGCCCCGA TGGCAGACAG 
CAGCTCATTA TTCCCGCCGC CGGGCAGAGG GAAGCCGGTC TGTATGAATG CGTAGCAGCC 
AATGCCCTGG GCTCAGTGGC CAGCTCCTGC ACAGTGTCTG TGGCAAGAAT ACCTGATCCC 
CCGGGGACCC CAGAGATCCC ACAGAAGTAT AAGAACACGG TGCTGGTTCT ATGGAAGACC 
CACGAGCACA ACTCCCCTTG CACCTACATC CTGGAGAAAC AGGTTAATGG TGATGGGGAA 
TGGAAGGTTC TAAGTTCTGG AATCACCGAC TGTTACTTCA ATGTGACGGA GCTCCAGGAA 
GGCACTGTCA GGTTCCGCGT CGCTTGTGCC AACAAAGCAG GAAAGGGGCC CTACAGCAAT 
GTGTCCAAGA CAATTGTCGT AGATGCAGAA GATCGTAAGC CAGGAACACC CTCTGTCCAG 
AAGGCACGGC CCGTGGGAGC TGCAGTGTCC ACTACTGTTC CAATGACTCT CCGGACCCCT 
TCTACCCCAG CTGTTGCGCC CCCTGTTGTC TCTACCCCCA AATTACCTCC ATCTTGTGGA 
TCTGCTCTTG CAGTTTCCTC AACAGTTATC ACTACAACCG CAACTGTGTC TCCAGCCCAG 
CCTCTCCCCA AAGGGCCTCC ACCTGCAACT CCTCCACGGA AACACCGAGG GATACTCTCC 
ACTCCCCCAA CCATGCACAA GCCTGTCATT GCCACCACTC TCAGTGCCGA TATTACTCCC 
CAAACTCTGC CCAATAACAG GGCTCCATCT TCTGTGGCTC CTCCTGCCGT ATGTTCTCAT 
GTCTCCACGG TGACTGTGTC TATGTCACCA GGTTCTTCGG TGACCCATTT AAGGGCCCCC 
TTCTCTACCC CCATGCAGAA ACCTACCCCA GAACCAATAA CAAAACCATT ACCAGTGTCA 
CAAGTACAAT CACCCAAAGC TCCTTCTGTG CCAACAGAGC AAACCCAGAA AATTGCCCTT 
GCCCCGGCTG TTGCCCAGGT TAAAGCACCT TTTGTGCCAA CTACTGCAAA AACTAACGAA 
CCATCACGTC TTCCATATAG TGTCCCTACG GAACCACAGA TGCCATCACA GTTAGCAGCT 
CCAAAACTAT CTGCCAAGTC CCCCTCTGTT CCAATTATGC TGCACATTCC CCCCTTTAAA 
TCAGCAACCC TACCTGCTTC CAGTCCCACT TCCAAAACCA TATCTTTTCC CTCTCCTCCT 
ACAAGCCCAG TAACCAAACC CTTAACACCT ACCTCCCCAG CTTATATGGT CACTTCCTTT 
ATATCAATGC CTGTGCCTTC ACCCTCTGTT GAGTCCTCCA CTCCACCTAC TCCACTTACC 
CCCATCACCC CACCTACCCC CATCACCCCA CCTACCCCAC TTACCCCCAC CACCCCTCCT 
ACCCCCATTA GCCCCACTTC CCCAACCGCC CCATTTACCC CCACTTCCCA AACTGTCCCT 
ACACGGGTGA TAGTAAGAAG TGTCACCCCC GGGAAGGATG CACGTTATAC ACCCGGAGGA 
AGAGTCACAC CCTCGGGGAG AGAAAGCACC ACCCTCCGAC AAGGGGTACC CCAAAAACCT 
TACACTTTCT TGGATGAGAA AGCAAGGGGG CGGTTTGGAG TGATCCGAGA GTGTAAGGAG 
AATGCAACTG GGAAGCACTT CATGGCAAAG ATAATCCAGT ACGAACCAGA AAACAAGCAA 
AACGTGCTGC AGGAGTATGA GATCCTCAAG GGTCTCCACC ATCAACGAAT CTTGAGTCTT 
CATGAAGCTT ATATTACCCC TCGCTACCTT GTGTTGATTT CTGAAAACTG CACAGGACGG 
GAACTGCTTT ACTGCCTAGT TGAGAGGTTC CGTTATTCTG AAGACGACGT GGTCAATTAC 
CTCCTGCAGA TCCTACAGGG GTTGGAGTAC CTCCATGAAC AAAAGGTCCT GCACCTCGAT 
ATCAAACCTG AGAACGTCAT TGTGTCCTAC ATGAACACTG TCAAGATCAT CGATTTGGGG 
AGTGCCCAGA ACTTTACACC GCTGGTCCTG CGACCCCTCG GCAAACGCAT AGGGACCCTG 
GAATATATGT CCCCCGAAAT GCTCAAAGGT GACGCAGTAG GCCCAGCAGC TGATATTTGG 
GGTGTAGGCG TTCTGACTTA CATAATGCTC AGCGGCCGCT CTCCGTTCTT TGAGCTGGAC 
CCCGTGGAAA CCGAGAACAA GATCTTGTCG GGACGATTTG ATATCTTTAA ATTGTATTCC 
AACGCCTCCC AAAGTGCTTC CCTCTTCATT CGCAAGATCC TGACTATATA TCCTTGGAGC 
CGCCCATCAC TTAAGGAATG TTTTTCCAAT CCCTGGCTCC AGGATGCCTA CCTAATGAAG 
CTTAGACGCC AAACCCTAAC CTTCACCACT AACAAGCTCA AAGAGTTCCT CGTGGAGCAA 
CAGAGGAGAC GCGCAGACAC AGCCACTAAG CACAAAGTCC TCCTTCGTTC GTACCACGGA 
AGCCAGCCCT CAGCTCCTGT CACTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031748704.1
CDS	445..11694
Translation	MHRAYGRRNLARNEEEAPVSPTQTFRSPPSPGIPPKHPKLAAEPGQPSPPQFVRKLKNAAIGTGCDICLKVAVAGHPLPTLNWYKNQERIQPGGEEYGTLCIRDSRMEDAGVYTCVAQNPLGEAITSAVLAIIELEDSEPGEEDAGGPQLQRTIRAPNNTPLGTPSGDSDTLLASSLHTTPSTLTALSQTDELSDWSGSQQTVLERDPRNARFLPEGGLRRGSINGSVTDSPRNFVNPRPPIIPPPSPRIGQRPAVIPAGRGPPTPVTPRKKTTMPNQYQDTVTEELEEKIKKPKSSVGSQASGQDSRPVTPLSETSGRVSILRASPKLVRSGSKIFEKLRCLEERRKSLDQTDSPFPVHSWLPLRKARSFDQPGAEGLGAGLGSSNEELRDDLRDGVRSEIGGFTLRYPSLRHKTTSLDDRGALYGRISDIETRFSQELTRIKRTVSQQQLVRSSQDLSGRSPSPQRSLAAQPAPVDPKLTKVSTAPKPSETEEKQPARKPLVRSFATVNQLSEIKEPLGFSSQPPTLPSKSSNGSAVYQDAVRHSETNMKSQPHLTGKIIAPKTVSPQVFKAMTLPAQSQKEAVGDTQPKRVPDVGSVPPPPSTHAPAALEIAQRGLVKEKGTVSEEKKYLPWALPVGDNNNSQMPQGKGGGQAKKHSDAHTSSKSNKSGRSKGKSRRSRPLSPELESSDDSYVSAGEDPAEAPVFEIPLQSALVNAGTEVLLKCIISGNPSPEVFWRKDHVLLKNSPTHQIRVEGERHTLLLRWALPSDSGIYTVTARNEVGEASSCGALTVKPAPTKESPAHRGTPRDLLSPITSDEEYLSPQEDLSEPTTPQHKMAGKGPAFKAPPTFKMPLLDQKGFEGQEIVLSVQVEGEPKPIINWLKNKQQVKPGGRFRISEGAWGIFSLHIAGADKRDSGFYTCKAINEYGTKQCEAKIEVLAPPGSTSLEVLAPLQDVSVCAGEAAVFECVVSGPADMDVDWMFRGKLLQPALLDCKMRFNGKQCVLLLNSVHEDDSGVYTCKLSTARDELTCSAMLTVHPSLAPLFTRKLSDRDVIEGRTARLDCKISGTPPPVVTWTHFGQPIEESENIHVLREGGHHTLLIPHVANEDEGQYTATAHNKHGEAECSAELYVEEPRSATASHISKLEKMPSIPEEPEMQECEVEGVMMPDFLRPLQDIDVVEFREVQLECQVTGLPYPTITWYHNGQKIQSSDDRRMTQYKDIHRLVFPSVTHSHAGVYKSVISNKVGKAACYAHLYVTDLVPTPPDGPPIITSVTGRIVKLQWKPPKRLDPAIDLAQITYSVQQQAVGSPQWTVITTNLKDTCYCVRSLTKEHQYLFRVTTNTPTSHSKPSPPSESVCLFNRGPYLEEMPSILDRPEILYMVENQPLCITVTLNHVHAKVTWQRIDTVLSNVEGVYELSMPDDDQHSLTLFSPRKSDLGQLTFEAKNRYGSDSCTISIELAEAPRFESIMEDIEIGVGETARFVVVVEGKPIPDIMWYKDGVLLAESGHFSFVYDDTECSLVILNTALGDSGVYTCTARNLSGEISCKAELQVCPDEPGAGSAIQEIDILKVRRLTDFYNIHKEIGRGAFSYVRHVVEKSNGRDLAAKFIPFRGATRESARRERDILSKLRHDRIIFFYDAFEKRSALIIVMELCSQEELLERLTRKPTVSESEIRSFIRQILEGLDYLHHKNILHLDIKPENILMADTTSEQIRICDFGNAQELTPGDPQYCKYGTPEFVAPEIVNQMPISTVTDIWPVGVLAYLCLTGVSPFVGENDYTTLMNIRGYTVAFEEKMFVGLTREARGFLIKVLGNEKLRPNAEESLEHPWFKTLAKGKSISTDHLKLFQSRRKWQRSLISYKSNMVMRTIPELLQDTSNHLSIAVPKNSKEASGLSSSSDSDDLEELPFVPMPLQVQFSGSRMSLNEIPTDEEQLSQPQDNGSCREDEEEGSQAVEISSTFKEQAVQQKEATVQASPLSKHAKGTLTRNRSAEVGSSSDEETSETEKREHPRRPLKKGSSLESADVPDGEIKAARRGELRRGSSADSALLLNVSTTDGDEERDRPDKGMIKAASMELPTRCRSPGRLEDRGTLRRKFGSADEEYAQRLEMMRQRLLRGGSADGKLSGLRGPLIETLSVDKKRAEQISIRSPRSDQSSLSPIPAIKLTRAASSEAAPGREATEERVLRKTSSFSHSDTEPLVLHRRSGAPLEIPLAQLEAQRLKESPSLSALTDRLESRPQTPREMIPKSLTPEAPQLKAESGEPETQSDKEAEKGTTPLIQHHKDKDSNSLKDLVIVLPRVTPPLDKMASKTELPSIQTERAQDKIPRADVNLPATTSSNETVPTKPPPLLLPSKQSLSVPPEPLPSSVLTNEMPKSKEEYSSSRAIKAKESISKTETTDQQDKIKEPLPQAVVPLPLPMPASQVLKPSTAKVQIADSPTMPENDASLPQITGKEKLSSPAVQPVDKTAAISSPYAAMIQTLQVPEEPETVVEECSKAPLKAQPVFQEIPIATGDAKAQGISQVNNKEGKDELSQEPKLINTRSSSTENVPFISNIDSEEIFEAKFKRNRENSLTRGLKKLTRTWSEEKNLAAAHASREEEMYRPSPVGVPLEFLVPAALGLHERSRSVQDLPDAERDSSFMRKLSLRFKKTPPTERKQKPAEEVEGTGRRLSWSIGRGSSKDKKDTESLNSETGSIETITDKHLKDQKKSSQSPVLAMRKKIGSTMDRLSMKLRSQSEDRRDAERTEKKEGRTDKSSPLMSLLRRSNSEGENLKKLGIPQNQLASQVSAPSTESIESGLSIKSEMVVKDDRRSRWDRWGLSRSKKEKMTSQPSIPASLMHEDGSIVGRQYIRNESDFPPVFHIKLKDIVVLEGEPVTLSCLPAGSPSPQILWKKDKMSLERGEKVHIKSCPDGRQQLIIPAAGQREAGLYECVAANALGSVASSCTVSVARIPDPPGTPEIPQKYKNTVLVLWKTHEHNSPCTYILEKQVNGDGEWKVLSSGITDCYFNVTELQEGTVRFRVACANKAGKGPYSNVSKTIVVDAEDRKPGTPSVQKARPVGAAVSTTVPMTLRTPSTPAVAPPVVSTPKLPPSCGSALAVSSTVITTTATVSPAQPLPKGPPPATPPRKHRGILSTPPTMHKPVIATTLSADITPQTLPNNRAPSSVAPPAVCSHVSTVTVSMSPGSSVTHLRAPFSTPMQKPTPEPITKPLPVSQVQSPKAPSVPTEQTQKIALAPAVAQVKAPFVPTTAKTNEPSRLPYSVPTEPQMPSQLAAPKLSAKSPSVPIMLHIPPFKSATLPASSPTSKTISFPSPPTSPVTKPLTPTSPAYMVTSFISMPVPSPSVESSTPPTPLTPITPPTPITPPTPLTPTTPPTPISPTSPTAPFTPTSQTVPTRVIVRSVTPGKDARYTPGGRVTPSGRESTTLRQGVPQKPYTFLDEKARGRFGVIRECKENATGKHFMAKIIQYEPENKQNVLQEYEILKGLHHQRILSLHEAYITPRYLVLISENCTGRELLYCLVERFRYSEDDVVNYLLQILQGLEYLHEQKVLHLDIKPENVIVSYMNTVKIIDLGSAQNFTPLVLRPLGKRIGTLEYMSPEMLKGDAVGPAADIWGVGVLTYIMLSGRSPFFELDPVETENKILSGRFDIFKLYSNASQSASLFIRKILTIYPWSRPSLKECFSNPWLQDAYLMKLRRQTLTFTTNKLKEFLVEQQRRRADTATKHKVLLRSYHGSQPSAPVTQ

Target ORF information:

RefSeq Version	XM_031892844.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis striated muscle enriched protein kinase (speg), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031892844.1

ORF Insert Sequence:

ATGCATCGTG CCTATGGGAG GAGGAACCTG GCCCGGAATG AAGAGGAAGC CCCTGTCTCC 
CCAACTCAAA CGTTTAGGTC TCCACCAAGC CCAGGCATTC CCCCAAAACA CCCCAAGTTG 
GCGGCTGAAC CTGGGCAGCC CTCTCCCCCT CAGTTTGTCC GGAAGCTGAA AAATGCTGCT 
ATAGGCACAG GCTGTGACAT CTGCCTAAAG GTGGCAGTGG CAGGGCACCC GCTGCCAACC 
CTAAACTGGT ACAAGAACCA GGAACGCATC CAACCCGGAG GAGAGGAATA TGGGACACTA 
TGCATCAGGG ACAGCAGGAT GGAAGATGCT GGAGTGTACA CCTGCGTGGC CCAGAATCCC 
CTGGGGGAGG CTATTACCTC TGCTGTGCTG GCAATCATTG AATTAGAAGA CTCAGAACCA 
GGGGAAGAAG ATGCAGGGGG CCCCCAATTA CAACGCACAA TTCGGGCACC CAACAATACG 
CCTCTGGGAA CCCCTTCAGG GGATTCAGAT ACGCTGCTGG CGAGCTCCCT GCACACCACG 
CCCAGCACGC TGACTGCACT CTCTCAAACA GACGAGCTCT CCGATTGGTC AGGCAGCCAA 
CAGACAGTAC TAGAAAGGGA CCCAAGGAAT GCAAGGTTCC TTCCGGAAGG GGGTCTGCGG 
AGGGGTAGCA TCAATGGTTC TGTAACAGAC TCCCCACGAA ACTTTGTCAA TCCTAGGCCG 
CCTATCATAC CACCACCCTC TCCGCGCATT GGCCAGCGTC CAGCTGTCAT TCCTGCAGGC 
CGTGGACCCC CCACCCCTGT TACACCTAGA AAGAAAACCA CAATGCCCAA CCAGTACCAG 
GACACAGTGA CTGAGGAGTT AGAAGAGAAG ATTAAGAAGC CAAAGTCCTC TGTTGGCTCT 
CAGGCTAGTG GCCAAGATTC TCGCCCTGTA ACTCCACTAA GTGAGACATC AGGGCGGGTG 
TCTATCCTTA GGGCATCCCC AAAGCTAGTC CGCTCAGGCT CTAAGATCTT TGAAAAGCTT 
AGGTGTCTCG AAGAGAGGAG AAAAAGTCTG GACCAGACAG ACAGCCCCTT TCCAGTGCAC 
TCATGGTTGC CTCTCCGTAA GGCACGATCC TTTGATCAGC CGGGGGCTGA GGGGCTTGGT 
GCTGGTCTAG GTTCCTCAAA CGAAGAGCTA AGGGATGATC TTAGAGACGG GGTAAGATCA 
GAAATAGGTG GGTTTACTCT GAGGTACCCC TCTCTGAGAC ACAAGACTAC CTCACTGGAT 
GACAGGGGAG CTCTCTATGG ACGAATAAGT GACATTGAGA CCCGCTTCTC ACAAGAGCTG 
ACTCGCATCA AAAGAACTGT GTCCCAGCAA CAGCTAGTCC GTTCCTCACA GGATCTATCT 
GGAAGATCAC CGTCACCCCA AAGATCCCTA GCTGCCCAGC CTGCTCCTGT GGACCCTAAG 
CTAACCAAAG TGTCAACGGC TCCAAAGCCT TCTGAAACAG AAGAGAAGCA GCCAGCAAGG 
AAGCCACTAG TCAGAAGCTT TGCCACCGTC AACCAACTGT CAGAAATTAA GGAACCTCTG 
GGGTTTTCAA GCCAACCCCC TACACTGCCA TCAAAATCAT CCAATGGTTC TGCAGTATAT 
CAGGATGCAG TGAGGCACTC GGAAACAAAT ATGAAATCAC AACCACACCT TACAGGAAAA 
ATCATTGCAC CAAAAACTGT TTCCCCACAA GTATTTAAAG CTATGACTCT CCCCGCACAA 
TCCCAAAAAG AGGCAGTCGG CGACACACAG CCGAAGAGAG TTCCTGATGT TGGTAGCGTG 
CCTCCGCCAC CCAGCACACA TGCCCCGGCA GCCCTAGAGA TAGCTCAGAG GGGACTTGTT 
AAAGAGAAGG GGACTGTAAG TGAAGAGAAG AAGTATCTAC CCTGGGCACT TCCTGTAGGA 
GACAATAACA ACAGCCAAAT GCCACAGGGT AAGGGTGGGG GACAGGCCAA AAAGCACAGC 
GATGCACACA CCAGCAGCAA AAGCAACAAA AGCGGCAGAA GCAAAGGGAA GAGTCGCCGA 
AGCAGGCCAT TGTCACCAGA ACTTGAGTCA TCGGATGATT CCTACGTATC GGCGGGAGAG 
GATCCAGCAG AGGCGCCTGT GTTTGAGATT CCTTTGCAAA GTGCACTAGT AAACGCCGGC 
ACTGAGGTCT TACTGAAATG TATTATATCT GGAAACCCAA GTCCAGAAGT GTTTTGGAGA 
AAGGATCACG TGTTGCTGAA AAACAGTCCG ACCCATCAAA TACGAGTAGA GGGCGAGAGA 
CACACGTTGC TACTGCGCTG GGCACTGCCC TCAGACTCGG GTATCTATAC AGTGACTGCG 
CGGAATGAAG TTGGGGAAGC CAGCAGCTGC GGTGCCCTCA CTGTAAAACC AGCACCTACC 
AAAGAGTCGC CGGCACATCG GGGAACACCT CGTGACCTGC TCAGCCCAAT AACATCGGAT 
GAAGAGTATC TAAGCCCCCA GGAAGATTTA AGTGAGCCTA CCACCCCCCA ACATAAGATG 
GCAGGCAAAG GCCCCGCATT CAAAGCTCCA CCCACTTTCA AGATGCCTCT TTTGGACCAG 
AAAGGGTTTG AAGGCCAAGA GATTGTTCTC AGCGTTCAGG TGGAAGGGGA ACCCAAGCCT 
ATTATCAACT GGCTGAAGAA CAAGCAGCAG GTGAAGCCTG GTGGAAGGTT CCGCATATCA 
GAAGGTGCTT GGGGGATCTT CTCGCTACAT ATTGCCGGGG CTGATAAACG AGATTCCGGA 
TTCTACACCT GTAAAGCAAT AAATGAGTAT GGGACAAAGC AATGTGAAGC CAAGATAGAG 
GTTCTAGCAC CCCCTGGCAG TACCAGCTTG GAGGTTCTGG CCCCCTTGCA GGATGTGAGT 
GTGTGCGCTG GGGAGGCAGC GGTGTTTGAA TGCGTGGTGT CGGGCCCCGC CGATATGGAC 
GTGGACTGGA TGTTCCGGGG CAAGCTGCTC CAGCCAGCAC TGCTGGACTG TAAGATGCGT 
TTCAACGGAA AGCAGTGTGT GCTGCTGCTG AATTCAGTCC ATGAAGATGA CAGTGGGGTC 
TACACCTGCA AACTGAGCAC TGCAAGAGAT GAGCTGACCT GTAGCGCTAT GCTAACTGTG 
CACCCATCGC TGGCGCCTCT CTTCACTCGC AAGTTGTCAG ATCGAGATGT TATAGAGGGA 
CGCACAGCTC GGCTGGACTG TAAAATCAGT GGCACCCCGC CCCCTGTTGT CACGTGGACT 
CATTTTGGAC AGCCCATAGA GGAAAGTGAG AACATCCACG TTTTGAGAGA AGGTGGACAT 
CACACCTTAC TGATACCCCA CGTGGCGAAT GAGGACGAGG GTCAGTACAC AGCGACTGCC 
CATAACAAGC ATGGGGAGGC CGAATGCTCT GCTGAACTCT ACGTGGAGGA GCCTCGTTCT 
GCTACAGCAT CTCATATCTC CAAGCTGGAG AAGATGCCAT CTATTCCAGA AGAGCCAGAG 
ATGCAGGAGT GTGAGGTAGA GGGTGTGATG ATGCCGGATT TCCTGCGACC TCTGCAGGAC 
ATTGACGTGG TGGAATTTCG GGAGGTACAA CTTGAGTGTC AGGTAACCGG TCTGCCGTAC 
CCTACCATCA CCTGGTATCA CAATGGTCAG AAGATCCAGA GCTCTGATGA TCGCAGAATG 
ACTCAATATA AAGACATCCA CCGCCTGGTT TTCCCCTCTG TTACTCATTC TCATGCCGGA 
GTCTATAAAA GTGTCATCTC AAACAAAGTG GGGAAAGCAG CCTGCTATGC GCACCTGTAT 
GTCACAGATT TGGTACCCAC TCCTCCAGAT GGTCCCCCCA TCATTACCTC TGTAACTGGG 
AGAATTGTGA AGCTCCAGTG GAAGCCACCC AAGAGATTGG ACCCAGCCAT TGATCTGGCT 
CAGATTACAT ACTCAGTCCA GCAGCAAGCG GTGGGATCTC CCCAATGGAC AGTCATCACC 
ACCAACCTAA AAGACACTTG TTATTGTGTC AGATCTCTGA CCAAGGAACA TCAGTATCTT 
TTCCGTGTGA CCACAAACAC CCCAACTTCT CACAGTAAAC CTTCCCCTCC CAGTGAAAGC 
GTTTGTCTCT TTAATAGAGG TCCCTACCTG GAGGAGATGC CTTCTATACT GGATCGGCCT 
GAAATCTTGT ATATGGTGGA AAATCAGCCT CTTTGCATAA CCGTGACACT GAACCACGTA 
CATGCCAAAG TCACGTGGCA GAGAATAGAC ACCGTTCTGA GCAATGTCGA GGGTGTGTAT 
GAGCTGAGTA TGCCGGATGA CGACCAGCAC TCGCTCACGT TGTTCAGCCC AAGGAAGTCT 
GACCTGGGGC AGCTGACATT TGAGGCTAAA AACCGCTACG GATCAGATAG CTGCACTATT 
TCCATCGAGC TCGCAGAGGC TCCTCGCTTT GAGTCCATCA TGGAAGACAT AGAGATCGGT 
GTTGGTGAAA CAGCTCGTTT TGTGGTCGTG GTGGAAGGGA AGCCTATTCC AGACATCATG 
TGGTACAAAG ATGGGGTACT TTTGGCAGAG AGCGGTCACT TCAGCTTTGT GTATGATGAT 
ACCGAGTGCT CTCTGGTGAT CTTAAACACG GCACTTGGTG ACTCCGGTGT GTACACCTGC 
ACGGCACGGA ACCTTTCAGG GGAAATTTCC TGCAAAGCGG AGCTGCAAGT GTGCCCAGAT 
GAACCAGGGG CAGGATCTGC AATACAGGAA ATAGATATAC TCAAAGTTCG TAGATTGACT 
GATTTCTACA ACATCCATAA AGAAATTGGC AGAGGGGCCT TCTCCTATGT CCGACATGTG 
GTAGAAAAGA GCAACGGGCG AGATCTTGCT GCTAAATTCA TACCGTTCCG TGGGGCAACC 
AGGGAGAGTG CACGGAGGGA GCGGGACATA CTGTCCAAGC TCAGACACGA CAGGATCATC 
TTCTTCTACG ATGCATTTGA GAAAAGAAGT GCTCTAATCA TTGTCATGGA ACTCTGTTCT 
CAAGAGGAGC TCCTAGAGAG GTTGACAAGA AAGCCTACAG TGAGCGAATC AGAGATCCGC 
TCGTTTATAC GGCAAATCCT AGAAGGGTTA GATTATTTGC ACCATAAAAA CATTCTGCAT 
TTGGACATAA AGCCAGAGAA CATCCTGATG GCCGATACGA CTAGTGAACA GATTCGCATT 
TGTGACTTTG GAAACGCTCA GGAACTAACC CCGGGGGATC CTCAGTACTG CAAATACGGA 
ACCCCGGAAT TTGTGGCGCC CGAAATTGTG AACCAGATGC CAATTTCTAC AGTAACGGAC 
ATATGGCCAG TTGGAGTCCT GGCATATTTA TGCCTTACCG GAGTGTCGCC CTTTGTTGGG 
GAGAATGATT ACACCACTCT GATGAATATC CGGGGTTACA CTGTGGCATT TGAAGAGAAG 
ATGTTTGTTG GTCTCACACG AGAAGCACGT GGTTTCCTAA TCAAAGTCTT GGGCAATGAG 
AAGCTGAGGC CTAATGCTGA AGAGAGTTTG GAGCACCCCT GGTTCAAAAC TTTAGCGAAA 
GGGAAAAGCA TCAGCACAGA CCATCTGAAG CTGTTCCAGT CTCGCCGTAA ATGGCAGCGC 
TCACTTATCA GCTATAAGTC CAATATGGTG ATGAGAACCA TTCCAGAGCT GCTTCAGGAC 
ACAAGCAATC ACTTGTCCAT TGCTGTACCC AAGAATTCCA AGGAAGCATC CGGATTGTCA 
TCCTCCTCTG ATTCTGATGA CCTGGAAGAG CTTCCCTTTG TCCCTATGCC TCTCCAGGTC 
CAATTCTCTG GATCACGCAT GTCCTTGAAT GAGATCCCAA CAGATGAGGA ACAACTGTCT 
CAGCCCCAGG ACAATGGCAG CTGTAGAGAA GATGAGGAAG AAGGAAGTCA GGCAGTGGAA 
ATTAGTAGCA CATTTAAAGA ACAAGCAGTT CAGCAGAAAG AAGCAACAGT CCAAGCAAGT 
CCATTGTCCA AGCATGCCAA AGGAACCCTT ACAAGGAACA GGTCTGCTGA AGTTGGTTCT 
TCTTCTGATG AGGAAACTTC AGAAACTGAA AAAAGAGAGC ATCCTCGTAG GCCTCTGAAA 
AAAGGTTCCA GCTTAGAATC TGCCGACGTT CCTGATGGGG AAATTAAAGC AGCTCGTAGA 
GGAGAGCTGA GGAGGGGAAG CTCTGCAGAT AGTGCTCTTT TGCTCAATGT GTCTACAACA 
GATGGAGATG AAGAGAGAGA TCGACCAGAC AAGGGAATGA TAAAAGCTGC CTCAATGGAG 
CTACCAACCA GATGCAGGAG CCCAGGTAGA TTGGAGGATC GAGGCACACT ACGAAGAAAG 
TTTGGATCAG CTGATGAAGA ATATGCTCAG CGGTTAGAAA TGATGAGACA GAGGCTATTG 
AGGGGAGGTT CGGCAGATGG AAAACTCAGT GGTCTTAGAG GCCCACTCAT AGAAACACTT 
TCTGTAGACA AGAAGCGAGC AGAGCAGATA TCTATACGCT CCCCAAGAAG TGATCAGTCT 
TCTCTGAGTC CTATTCCCGC CATCAAACTA ACCCGAGCTG CTTCCAGTGA GGCTGCGCCT 
GGTAGAGAAG CAACTGAAGA AAGAGTCTTA AGGAAAACCA GTTCCTTTTC TCACAGCGAC 
ACAGAGCCAC TGGTCTTGCA TCGCAGGTCA GGGGCACCCC TAGAAATACC TCTTGCTCAG 
TTAGAGGCAC AGCGTCTTAA AGAGTCTCCC TCTCTTTCAG CATTGACAGA CCGATTAGAA 
TCACGTCCTC AAACACCGCG AGAGATGATT CCCAAATCTC TAACTCCAGA AGCACCGCAA 
TTGAAGGCAG AAAGTGGGGA GCCAGAAACT CAGAGTGATA AGGAAGCTGA GAAGGGCACC 
ACACCTCTTA TTCAGCATCA TAAAGACAAA GACTCAAATT CATTGAAGGA CTTGGTTATT 
GTTCTTCCAA GAGTGACTCC ACCTTTGGAT AAAATGGCAT CTAAAACAGA GTTACCAAGT 
ATTCAAACAG AGAGGGCACA GGACAAAATC CCTAGAGCAG ATGTGAACCT GCCAGCTACT 
ACTTCATCAA ATGAAACTGT GCCAACAAAG CCACCACCCT TACTTTTGCC TTCTAAGCAG 
TCTCTTTCTG TGCCACCTGA ACCTTTACCC TCTTCAGTAC TGACAAATGA GATGCCTAAA 
TCTAAAGAGG AGTACTCGTC ATCAAGGGCC ATTAAGGCCA AGGAATCCAT ATCAAAAACA 
GAAACAACAG ACCAGCAAGA TAAAATTAAA GAACCTTTAC CACAAGCTGT TGTTCCATTA 
CCATTGCCTA TGCCAGCCTC CCAAGTGCTA AAACCTTCCA CTGCAAAAGT ACAGATAGCA 
GATTCTCCAA CAATGCCTGA GAATGATGCA TCACTTCCAC AGATAACAGG TAAAGAGAAA 
CTATCTTCTC CAGCTGTCCA ACCAGTAGAT AAAACAGCAG CTATATCATC ACCTTATGCT 
GCTATGATTC AAACACTTCA AGTGCCCGAG GAACCTGAGA CAGTTGTTGA GGAATGTTCA 
AAGGCCCCAT TAAAAGCTCA GCCAGTATTT CAAGAAATTC CTATAGCCAC TGGAGATGCA 
AAGGCTCAAG GAATATCACA AGTAAACAAT AAAGAAGGCA AGGATGAGCT ATCACAAGAA 
CCCAAGCTGA TTAACACCAG ATCTTCCAGC ACAGAGAATG TCCCATTTAT CAGCAACATT 
GATTCAGAAG AAATCTTTGA GGCTAAGTTC AAGAGGAACC GTGAAAATTC TCTTACCCGA 
GGTCTAAAAA AGCTTACCAG GACTTGGTCA GAGGAGAAGA ATTTAGCTGC AGCCCATGCA 
TCTCGGGAAG AAGAAATGTA CAGGCCTAGT CCTGTGGGTG TCCCACTAGA GTTCCTTGTT 
CCTGCAGCTC TTGGGCTACA TGAAAGATCA AGATCAGTGC AAGATCTGCC AGATGCTGAG 
AGGGATTCAA GCTTTATGAG AAAGCTTTCT TTGAGATTTA AGAAAACTCC ACCAACTGAA 
AGGAAGCAGA AACCAGCAGA AGAAGTAGAA GGTACTGGTA GACGACTTTC ATGGTCAATA 
GGTAGAGGAA GTTCCAAAGA TAAGAAGGAT ACAGAGAGCC TGAACTCTGA AACAGGCTCA 
ATAGAAACCA TCACAGACAA ACATCTAAAG GACCAGAAAA AGAGTAGTCA ATCACCAGTG 
TTGGCCATGA GAAAGAAAAT AGGCAGTACT ATGGATCGAC TTTCTATGAA ACTAAGGAGC 
CAATCAGAAG ATAGAAGGGA TGCAGAGAGA ACAGAGAAGA AAGAAGGGCG TACAGACAAA 
AGTTCTCCAT TAATGTCTTT ACTTAGGAGA TCCAACTCAG AAGGAGAGAA CTTAAAGAAA 
CTAGGTATTC CCCAGAACCA ACTTGCTTCC CAGGTTTCAG CTCCATCAAC TGAGTCTATT 
GAATCTGGTC TCAGCATCAA ATCTGAAATG GTAGTCAAGG ATGATAGGCG CTCAAGGTGG 
GATCGTTGGG GCCTGTCACG TAGCAAAAAG GAGAAGATGA CATCGCAGCC AAGTATTCCA 
GCCAGTCTGA TGCATGAAGA TGGCAGTATT GTTGGCAGAC AATACATTCG TAATGAGTCA 
GACTTCCCCC CAGTCTTCCA TATCAAGCTG AAGGACATCG TGGTGCTAGA GGGAGAGCCG 
GTGACTCTGT CCTGCTTACC TGCTGGCAGC CCTTCCCCCC AGATCCTGTG GAAGAAAGAT 
AAGATGTCCT TGGAAAGAGG AGAAAAGGTG CATATAAAAT CCTGCCCCGA TGGCAGACAG 
CAGCTCATTA TTCCCGCCGC CGGGCAGAGG GAAGCCGGTC TGTATGAATG CGTAGCAGCC 
AATGCCCTGG GCTCAGTGGC CAGCTCCTGC ACAGTGTCTG TGGCAAGAAT ACCTGATCCC 
CCGGGGACCC CAGAGATCCC ACAGAAGTAT AAGAACACGG TGCTGGTTCT ATGGAAGACC 
CACGAGCACA ACTCCCCTTG CACCTACATC CTGGAGAAAC AGGTTAATGG TGATGGGGAA 
TGGAAGGTTC TAAGTTCTGG AATCACCGAC TGTTACTTCA ATGTGACGGA GCTCCAGGAA 
GGCACTGTCA GGTTCCGCGT CGCTTGTGCC AACAAAGCAG GAAAGGGGCC CTACAGCAAT 
GTGTCCAAGA CAATTGTCGT AGATGCAGAA GATCGTAAGC CAGGAACACC CTCTGTCCAG 
AAGGCACGGC CCGTGGGAGC TGCAGTGTCC ACTACTGTTC CAATGACTCT CCGGACCCCT 
TCTACCCCAG CTGTTGCGCC CCCTGTTGTC TCTACCCCCA AATTACCTCC ATCTTGTGGA 
TCTGCTCTTG CAGTTTCCTC AACAGTTATC ACTACAACCG CAACTGTGTC TCCAGCCCAG 
CCTCTCCCCA AAGGGCCTCC ACCTGCAACT CCTCCACGGA AACACCGAGG GATACTCTCC 
ACTCCCCCAA CCATGCACAA GCCTGTCATT GCCACCACTC TCAGTGCCGA TATTACTCCC 
CAAACTCTGC CCAATAACAG GGCTCCATCT TCTGTGGCTC CTCCTGCCGT ATGTTCTCAT 
GTCTCCACGG TGACTGTGTC TATGTCACCA GGTTCTTCGG TGACCCATTT AAGGGCCCCC 
TTCTCTACCC CCATGCAGAA ACCTACCCCA GAACCAATAA CAAAACCATT ACCAGTGTCA 
CAAGTACAAT CACCCAAAGC TCCTTCTGTG CCAACAGAGC AAACCCAGAA AATTGCCCTT 
GCCCCGGCTG TTGCCCAGGT TAAAGCACCT TTTGTGCCAA CTACTGCAAA AACTAACGAA 
CCATCACGTC TTCCATATAG TGTCCCTACG GAACCACAGA TGCCATCACA GTTAGCAGCT 
CCAAAACTAT CTGCCAAGTC CCCCTCTGTT CCAATTATGC TGCACATTCC CCCCTTTAAA 
TCAGCAACCC TACCTGCTTC CAGTCCCACT TCCAAAACCA TATCTTTTCC CTCTCCTCCT 
ACAAGCCCAG TAACCAAACC CTTAACACCT ACCTCCCCAG CTTATATGGT CACTTCCTTT 
ATATCAATGC CTGTGCCTTC ACCCTCTGTT GAGTCCTCCA CTCCACCTAC TCCACTTACC 
CCCATCACCC CACCTACCCC CATCACCCCA CCTACCCCAC TTACCCCCAC CACCCCTCCT 
ACCCCCATTA GCCCCACTTC CCCAACCGCC CCATTTACCC CCACTTCCCA AACTGTCCCT 
ACACGGGTGA TAGTAAGAAG TGTCACCCCC GGGAAGGATG CACGTTATAC ACCCGGAGGA 
AGAGTCACAC CCTCGGGGAG AGAAAGCACC ACCCTCCGAC AAGGGGTACC CCAAAAACCT 
TACACTTTCT TGGATGAGAA AGCAAGGGGG CGGTTTGGAG TGATCCGAGA GTGTAAGGAG 
AATGCAACTG GGAAGCACTT CATGGCAAAG ATAATCCAGT ACGAACCAGA AAACAAGCAA 
AACGTGCTGC AGGAGTATGA GATCCTCAAG GGTCTCCACC ATCAACGAAT CTTGAGTCTT 
CATGAAGCTT ATATTACCCC TCGCTACCTT GTGTTGATTT CTGAAAACTG CACAGGACGG 
GAACTGCTTT ACTGCCTAGT TGAGAGGTTC CGTTATTCTG AAGACGACGT GGTCAATTAC 
CTCCTGCAGA TCCTACAGGG GTTGGAGTAC CTCCATGAAC AAAAGGTCCT GCACCTCGAT 
ATCAAACCTG AGAACGTCAT TGTGTCCTAC ATGAACACTG TCAAGATCAT CGATTTGGGG 
AGTGCCCAGA ACTTTACACC GCTGGTCCTG CGACCCCTCG GCAAACGCAT AGGGACCCTG 
GAATATATGT CCCCCGAAAT GCTCAAAGGT GACGCAGTAG GCCCAGCAGC TGATATTTGG 
GGTGTAGGCG TTCTGACTTA CATAATGCTC AGCGGCCGCT CTCCGTTCTT TGAGCTGGAC 
CCCGTGGAAA CCGAGAACAA GATCTTGTCG GGACGATTTG ATATCTTTAA ATTGTATTCC 
AACGCCTCCC AAAGTGCTTC CCTCTTCATT CGCAAGATCC TGACTATATA TCCTTGGAGC 
CGCCCATCAC TTAAGGAATG TTTTTCCAAT CCCTGGCTCC AGGATGCCTA CCTAATGAAG 
CTTAGACGCC AAACCCTAAC CTTCACCACT AACAAGCTCA AAGAGTTCCT CGTGGAGCAA 
CAGAGGAGAC GCGCAGACAC AGCCACTAAG CACAAAGTCC TCCTTCGTTC GTACCACGGA 
AGCCAGCCCT CAGCTCCTGT CACTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

speg ( XM_031892845.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031748705.1 Xenopus tropicalis striated muscle enriched protein kinase (speg), transcript variant X3, mRNA.

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CloneID	OXa66224
Clone ID Related Accession (Same CDS sequence)	XM_031892845.1
Accession Version	XM_031892845.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11124bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	striated muscle preferentially expressed protein kinase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGA GTTGGATGAA AAGACGCTCC CAGAAATCGG GACACCCTCG GAGGGTCTTC 
GGACGCTTTA CTCATGTATT CCGCTCCTGC AGGAAACACA GCTACGACTC AGAACCAGGG 
GAAGAAGATG CAGGGGGCCC CCAATTACAA CGCACAATTC GGGCACCCAA CAATACGCCT 
CTGGGAACCC CTTCAGGGGA TTCAGATACG CTGCTGGCGA GCTCCCTGCA CACCACGCCC 
AGCACGCTGA CTGCACTCTC TCAAACAGAC GAGCTCTCCG ATTGGTCAGG CAGCCAACAG 
ACAGTACTAG AAAGGGACCC AAGGAATGCA AGGCAAGGCT TCACTCAGGG AGGAGGGGCT 
CGCCATCTGG GGGTGGAGCC CCTCATTCCA GCTTCCAGCA ACGAGTTCCC GGGCAGTGTC 
TGGGGGTCAG AGGTCAGCCT TTCTGGGTTC AGTGATCTGT ATAGCAGCGC CTTCAGCCTG 
TACCGAGGCA GATCTTGTGC CATCCGGGTG TTCCTTCCGG AAGGGGGTCT GCGGAGGGGT 
AGCATCAATG GTTCTGTAAC AGACTCCCCA CGAAACTTTG TCAATCCTAG GCCGCCTATC 
ATACCACCAC CCTCTCCGCG CATTGGCCAG CGTCCAGCTG TCATTCCTGC AGGCCGTGGA 
CCCCCCACCC CTGTTACACC TAGAAAGAAA ACCACAATGC CCAACCAGTA CCAGGACACA 
GTGACTGAGG AGTTAGAAGA GAAGATTAAG AAGCCAAAGT CCTCTGTTGG CTCTCAGGCT 
AGTGGCCAAG ATTCTCGCCC TGTAACTCCA CTAAGTGAGA CATCAGGGCG GGTGTCTATC 
CTTAGGGCAT CCCCAAAGCT AGTCCGCTCA GGCTCTAAGA TCTTTGAAAA GCTTAGGTGT 
CTCGAAGAGA GGAGAAAAAG TCTGGACCAG ACAGACAGCC CCTTTCCAGT GCACTCATGG 
TTGCCTCTCC GTAAGGCACG ATCCTTTGAT CAGCCGGGGG CTGAGGGGCT TGGTGCTGGT 
CTAGGTTCCT CAAACGAAGA GCTAAGGGAT GATCTTAGAG ACGGGGTAAG ATCAGAAATA 
GGTGGGTTTA CTCTGAGGTA CCCCTCTCTG AGACACAAGA CTACCTCACT GGATGACAGG 
GGAGCTCTCT ATGGACGAAT AAGTGACATT GAGACCCGCT TCTCACAAGA GCTGACTCGC 
ATCAAAAGAA CTGTGTCCCA GCAACAGCTA GTCCGTTCCT CACAGGATCT ATCTGGAAGA 
TCACCGTCAC CCCAAAGATC CCTAGCTGCC CAGCCTGCTC CTGTGGACCC TAAGCTAACC 
AAAGTGTCAA CGGCTCCAAA GCCTTCTGAA ACAGAAGAGA AGCAGCCAGC AAGGAAGCCA 
CTAGTCAGAA GCTTTGCCAC CGTCAACCAA CTGTCAGAAA TTAAGGAACC TCTGGGGTTT 
TCAAGCCAAC CCCCTACACT GCCATCAAAA TCATCCAATG GTTCTGCAGT ATATCAGGAT 
GCAGTGAGGC ACTCGGAAAC AAATATGAAA TCACAACCAC ACCTTACAGG AAAAATCATT 
GCACCAAAAA CTGTTTCCCC ACAAGTATTT AAAGCTATGA CTCTCCCCGC ACAATCCCAA 
AAAGAGGCAG TCGGCGACAC ACAGCCGAAG AGAGTTCCTG ATGTTGGTAG CGTGCCTCCG 
CCACCCAGCA CACATGCCCC GGCAGCCCTA GAGATAGCTC AGAGGGGACT TGTTAAAGAG 
AAGGGGACTG TAAGTGAAGA GAAGAAGTAT CTACCCTGGG CACTTCCTGT AGGAGACAAT 
AACAACAGCC AAATGCCACA GGGTAAGGGT GGGGGACAGG CCAAAAAGCA CAGCGATGCA 
CACACCAGCA GCAAAAGCAA CAAAAGCGGC AGAAGCAAAG GGAAGAGTCG CCGAAGCAGG 
CCATTGTCAC CAGAACTTGA GTCATCGGAT GATTCCTACG TATCGGCGGG AGAGGATCCA 
GCAGAGGCGC CTGTGTTTGA GATTCCTTTG CAAAGTGCAC TAGTAAACGC CGGCACTGAG 
GTCTTACTGA AATGTATTAT ATCTGGAAAC CCAAGTCCAG AAGTGTTTTG GAGAAAGGAT 
CACGTGTTGC TGAAAAACAG TCCGACCCAT CAAATACGAG TAGAGGGCGA GAGACACACG 
TTGCTACTGC GCTGGGCACT GCCCTCAGAC TCGGGTATCT ATACAGTGAC TGCGCGGAAT 
GAAGTTGGGG AAGCCAGCAG CTGCGGTGCC CTCACTGTAA AACCAGCACC TACCAAAGAG 
TCGCCGGCAC ATCGGGGAAC ACCTCGTGAC CTGCTCAGCC CAATAACATC GGATGAAGAG 
TATCTAAGCC CCCAGGAAGA TTTAAGTGAG CCTACCACCC CCCAACATAA GATGGCAGGC 
AAAGGCCCCG CATTCAAAGC TCCACCCACT TTCAAGATGC CTCTTTTGGA CCAGAAAGGG 
TTTGAAGGCC AAGAGATTGT TCTCAGCGTT CAGGTGGAAG GGGAACCCAA GCCTATTATC 
AACTGGCTGA AGAACAAGCA GCAGGTGAAG CCTGGTGGAA GGTTCCGCAT ATCAGAAGGT 
GCTTGGGGGA TCTTCTCGCT ACATATTGCC GGGGCTGATA AACGAGATTC CGGATTCTAC 
ACCTGTAAAG CAATAAATGA GTATGGGACA AAGCAATGTG AAGCCAAGAT AGAGGTTCTA 
GCACCCCCTG GCAGTACCAG CTTGGAGGTT CTGGCCCCCT TGCAGGATGT GAGTGTGTGC 
GCTGGGGAGG CAGCGGTGTT TGAATGCGTG GTGTCGGGCC CCGCCGATAT GGACGTGGAC 
TGGATGTTCC GGGGCAAGCT GCTCCAGCCA GCACTGCTGG ACTGTAAGAT GCGTTTCAAC 
GGAAAGCAGT GTGTGCTGCT GCTGAATTCA GTCCATGAAG ATGACAGTGG GGTCTACACC 
TGCAAACTGA GCACTGCAAG AGATGAGCTG ACCTGTAGCG CTATGCTAAC TGTGCACCCA 
TCGCTGGCGC CTCTCTTCAC TCGCAAGTTG TCAGATCGAG ATGTTATAGA GGGACGCACA 
GCTCGGCTGG ACTGTAAAAT CAGTGGCACC CCGCCCCCTG TTGTCACGTG GACTCATTTT 
GGACAGCCCA TAGAGGAAAG TGAGAACATC CACGTTTTGA GAGAAGGTGG ACATCACACC 
TTACTGATAC CCCACGTGGC GAATGAGGAC GAGGGTCAGT ACACAGCGAC TGCCCATAAC 
AAGCATGGGG AGGCCGAATG CTCTGCTGAA CTCTACGTGG AGGAGCCTCG TTCTGCTACA 
GCATCTCATA TCTCCAAGCT GGAGAAGATG CCATCTATTC CAGAAGAGCC AGAGATGCAG 
GAGTGTGAGG TAGAGGGTGT GATGATGCCG GATTTCCTGC GACCTCTGCA GGACATTGAC 
GTGGTGGAAT TTCGGGAGGT ACAACTTGAG TGTCAGGTAA CCGGTCTGCC GTACCCTACC 
ATCACCTGGT ATCACAATGG TCAGAAGATC CAGAGCTCTG ATGATCGCAG AATGACTCAA 
TATAAAGACA TCCACCGCCT GGTTTTCCCC TCTGTTACTC ATTCTCATGC CGGAGTCTAT 
AAAAGTGTCA TCTCAAACAA AGTGGGGAAA GCAGCCTGCT ATGCGCACCT GTATGTCACA 
GATTTGGTAC CCACTCCTCC AGATGGTCCC CCCATCATTA CCTCTGTAAC TGGGAGAATT 
GTGAAGCTCC AGTGGAAGCC ACCCAAGAGA TTGGACCCAG CCATTGATCT GGCTCAGATT 
ACATACTCAG TCCAGCAGCA AGCGGTGGGA TCTCCCCAAT GGACAGTCAT CACCACCAAC 
CTAAAAGACA CTTGTTATTG TGTCAGATCT CTGACCAAGG AACATCAGTA TCTTTTCCGT 
GTGACCACAA ACACCCCAAC TTCTCACAGT AAACCTTCCC CTCCCAGTGA AAGCGTTTGT 
CTCTTTAATA GAGGTCCCTA CCTGGAGGAG ATGCCTTCTA TACTGGATCG GCCTGAAATC 
TTGTATATGG TGGAAAATCA GCCTCTTTGC ATAACCGTGA CACTGAACCA CGTACATGCC 
AAAGTCACGT GGCAGAGAAT AGACACCGTT CTGAGCAATG TCGAGGGTGT GTATGAGCTG 
AGTATGCCGG ATGACGACCA GCACTCGCTC ACGTTGTTCA GCCCAAGGAA GTCTGACCTG 
GGGCAGCTGA CATTTGAGGC TAAAAACCGC TACGGATCAG ATAGCTGCAC TATTTCCATC 
GAGCTCGCAG AGGCTCCTCG CTTTGAGTCC ATCATGGAAG ACATAGAGAT CGGTGTTGGT 
GAAACAGCTC GTTTTGTGGT CGTGGTGGAA GGGAAGCCTA TTCCAGACAT CATGTGGTAC 
AAAGATGGGG TACTTTTGGC AGAGAGCGGT CACTTCAGCT TTGTGTATGA TGATACCGAG 
TGCTCTCTGG TGATCTTAAA CACGGCACTT GGTGACTCCG GTGTGTACAC CTGCACGGCA 
CGGAACCTTT CAGGGGAAAT TTCCTGCAAA GCGGAGCTGC AAGTGTGCCC AGATGAACCA 
GGGGCAGGAT CTGCAATACA GGAAATAGAT ATACTCAAAG TTCGTAGATT GACTGATTTC 
TACAACATCC ATAAAGAAAT TGGCAGAGGG GCCTTCTCCT ATGTCCGACA TGTGGTAGAA 
AAGAGCAACG GGCGAGATCT TGCTGCTAAA TTCATACCGT TCCGTGGGGC AACCAGGGAG 
AGTGCACGGA GGGAGCGGGA CATACTGTCC AAGCTCAGAC ACGACAGGAT CATCTTCTTC 
TACGATGCAT TTGAGAAAAG AAGTGCTCTA ATCATTGTCA TGGAACTCTG TTCTCAAGAG 
GAGCTCCTAG AGAGGTTGAC AAGAAAGCCT ACAGTGAGCG AATCAGAGAT CCGCTCGTTT 
ATACGGCAAA TCCTAGAAGG GTTAGATTAT TTGCACCATA AAAACATTCT GCATTTGGAC 
ATAAAGCCAG AGAACATCCT GATGGCCGAT ACGACTAGTG AACAGATTCG CATTTGTGAC 
TTTGGAAACG CTCAGGAACT AACCCCGGGG GATCCTCAGT ACTGCAAATA CGGAACCCCG 
GAATTTGTGG CGCCCGAAAT TGTGAACCAG ATGCCAATTT CTACAGTAAC GGACATATGG 
CCAGTTGGAG TCCTGGCATA TTTATGCCTT ACCGGAGTGT CGCCCTTTGT TGGGGAGAAT 
GATTACACCA CTCTGATGAA TATCCGGGGT TACACTGTGG CATTTGAAGA GAAGATGTTT 
GTTGGTCTCA CACGAGAAGC ACGTGGTTTC CTAATCAAAG TCTTGGGCAA TGAGAAGCTG 
AGGCCTAATG CTGAAGAGAG TTTGGAGCAC CCCTGGTTCA AAACTTTAGC GAAAGGGAAA 
AGCATCAGCA CAGACCATCT GAAGCTGTTC CAGTCTCGCC GTAAATGGCA GCGCTCACTT 
ATCAGCTATA AGTCCAATAT GGTGATGAGA ACCATTCCAG AGCTGCTTCA GGACACAAGC 
AATCACTTGT CCATTGCTGT ACCCAAGAAT TCCAAGGAAG CATCCGGATT GTCATCCTCC 
TCTGATTCTG ATGACCTGGA AGAGCTTCCC TTTGTCCCTA TGCCTCTCCA GGTCCAATTC 
TCTGGATCAC GCATGTCCTT GAATGAGATC CCAACAGATG AGGAACAACT GTCTCAGCCC 
CAGGACAATG GCAGCTGTAG AGAAGATGAG GAAGAAGGAA GTCAGGCAGT GGAAATTAGT 
AGCACATTTA AAGAACAAGC AGTTCAGCAG AAAGAAGCAA CAGTCCAAGC AAGTCCATTG 
TCCAAGCATG CCAAAGGAAC CCTTACAAGG AACAGGTCTG CTGAAGTTGG TTCTTCTTCT 
GATGAGGAAA CTTCAGAAAC TGAAAAAAGA GAGCATCCTC GTAGGCCTCT GAAAAAAGGT 
TCCAGCTTAG AATCTGCCGA CGTTCCTGAT GGGGAAATTA AAGCAGCTCG TAGAGGAGAG 
CTGAGGAGGG GAAGCTCTGC AGATAGTGCT CTTTTGCTCA ATGTGTCTAC AACAGATGGA 
GATGAAGAGA GAGATCGACC AGACAAGGGA ATGATAAAAG CTGCCTCAAT GGAGCTACCA 
ACCAGATGCA GGAGCCCAGG TAGATTGGAG GATCGAGGCA CACTACGAAG AAAGTTTGGA 
TCAGCTGATG AAGAATATGC TCAGCGGTTA GAAATGATGA GACAGAGGCT ATTGAGGGGA 
GGTTCGGCAG ATGGAAAACT CAGTGGTCTT AGAGGCCCAC TCATAGAAAC ACTTTCTGTA 
GACAAGAAGC GAGCAGAGCA GATATCTATA CGCTCCCCAA GAAGTGATCA GTCTTCTCTG 
AGTCCTATTC CCGCCATCAA ACTAACCCGA GCTGCTTCCA GTGAGGCTGC GCCTGGTAGA 
GAAGCAACTG AAGAAAGAGT CTTAAGGAAA ACCAGTTCCT TTTCTCACAG CGACACAGAG 
CCACTGGTCT TGCATCGCAG GTCAGGGGCA CCCCTAGAAA TACCTCTTGC TCAGTTAGAG 
GCACAGCGTC TTAAAGAGTC TCCCTCTCTT TCAGCATTGA CAGACCGATT AGAATCACGT 
CCTCAAACAC CGCGAGAGAT GATTCCCAAA TCTCTAACTC CAGAAGCACC GCAATTGAAG 
GCAGAAAGTG GGGAGCCAGA AACTCAGAGT GATAAGGAAG CTGAGAAGGG CACCACACCT 
CTTATTCAGC ATCATAAAGA CAAAGACTCA AATTCATTGA AGGACTTGGT TATTGTTCTT 
CCAAGAGTGA CTCCACCTTT GGATAAAATG GCATCTAAAA CAGAGTTACC AAGTATTCAA 
ACAGAGAGGG CACAGGACAA AATCCCTAGA GCAGATGTGA ACCTGCCAGC TACTACTTCA 
TCAAATGAAA CTGTGCCAAC AAAGCCACCA CCCTTACTTT TGCCTTCTAA GCAGTCTCTT 
TCTGTGCCAC CTGAACCTTT ACCCTCTTCA GTACTGACAA ATGAGATGCC TAAATCTAAA 
GAGGAGTACT CGTCATCAAG GGCCATTAAG GCCAAGGAAT CCATATCAAA AACAGAAACA 
ACAGACCAGC AAGATAAAAT TAAAGAACCT TTACCACAAG CTGTTGTTCC ATTACCATTG 
CCTATGCCAG CCTCCCAAGT GCTAAAACCT TCCACTGCAA AAGTACAGAT AGCAGATTCT 
CCAACAATGC CTGAGAATGA TGCATCACTT CCACAGATAA CAGGTAAAGA GAAACTATCT 
TCTCCAGCTG TCCAACCAGT AGATAAAACA GCAGCTATAT CATCACCTTA TGCTGCTATG 
ATTCAAACAC TTCAAGTGCC CGAGGAACCT GAGACAGTTG TTGAGGAATG TTCAAAGGCC 
CCATTAAAAG CTCAGCCAGT ATTTCAAGAA ATTCCTATAG CCACTGGAGA TGCAAAGGCT 
CAAGGAATAT CACAAGTAAA CAATAAAGAA GGCAAGGATG AGCTATCACA AGAACCCAAG 
CTGATTAACA CCAGATCTTC CAGCACAGAG AATGTCCCAT TTATCAGCAA CATTGATTCA 
GAAGAAATCT TTGAGGCTAA GTTCAAGAGG AACCGTGAAA ATTCTCTTAC CCGAGGTCTA 
AAAAAGCTTA CCAGGACTTG GTCAGAGGAG AAGAATTTAG CTGCAGCCCA TGCATCTCGG 
GAAGAAGAAA TGTACAGGCC TAGTCCTGTG GGTGTCCCAC TAGAGTTCCT TGTTCCTGCA 
GCTCTTGGGC TACATGAAAG ATCAAGATCA GTGCAAGATC TGCCAGATGC TGAGAGGGAT 
TCAAGCTTTA TGAGAAAGCT TTCTTTGAGA TTTAAGAAAA CTCCACCAAC TGAAAGGAAG 
CAGAAACCAG CAGAAGAAGT AGAAGGTACT GGTAGACGAC TTTCATGGTC AATAGGTAGA 
GGAAGTTCCA AAGATAAGAA GGATACAGAG AGCCTGAACT CTGAAACAGG CTCAATAGAA 
ACCATCACAG ACAAACATCT AAAGGACCAG AAAAAGAGTA GTCAATCACC AGTGTTGGCC 
ATGAGAAAGA AAATAGGCAG TACTATGGAT CGACTTTCTA TGAAACTAAG GAGCCAATCA 
GAAGATAGAA GGGATGCAGA GAGAACAGAG AAGAAAGAAG GGCGTACAGA CAAAAGTTCT 
CCATTAATGT CTTTACTTAG GAGATCCAAC TCAGAAGGAG AGAACTTAAA GAAACTAGGT 
ATTCCCCAGA ACCAACTTGC TTCCCAGGTT TCAGCTCCAT CAACTGAGTC TATTGAATCT 
GGTCTCAGCA TCAAATCTGA AATGGTAGTC AAGGATGATA GGCGCTCAAG GTGGGATCGT 
TGGGGCCTGT CACGTAGCAA AAAGGAGAAG ATGACATCGC AGCCAAGTAT TCCAGCCAGT 
CTGATGCATG AAGATGGCAG TATTGTTGGC AGACAATACA TTCGTAATGA GTCAGACTTC 
CCCCCAGTCT TCCATATCAA GCTGAAGGAC ATCGTGGTGC TAGAGGGAGA GCCGGTGACT 
CTGTCCTGCT TACCTGCTGG CAGCCCTTCC CCCCAGATCC TGTGGAAGAA AGATAAGATG 
TCCTTGGAAA GAGGAGAAAA GGTGCATATA AAATCCTGCC CCGATGGCAG ACAGCAGCTC 
ATTATTCCCG CCGCCGGGCA GAGGGAAGCC GGTCTGTATG AATGCGTAGC AGCCAATGCC 
CTGGGCTCAG TGGCCAGCTC CTGCACAGTG TCTGTGGCAA GAATACCTGA TCCCCCGGGG 
ACCCCAGAGA TCCCACAGAA GTATAAGAAC ACGGTGCTGG TTCTATGGAA GACCCACGAG 
CACAACTCCC CTTGCACCTA CATCCTGGAG AAACAGGTTA ATGGTGATGG GGAATGGAAG 
GTTCTAAGTT CTGGAATCAC CGACTGTTAC TTCAATGTGA CGGAGCTCCA GGAAGGCACT 
GTCAGGTTCC GCGTCGCTTG TGCCAACAAA GCAGGAAAGG GGCCCTACAG CAATGTGTCC 
AAGACAATTG TCGTAGATGC AGAAGATCGT AAGCCAGGAA CACCCTCTGT CCAGAAGGCA 
CGGCCCGTGG GAGCTGCAGT GTCCACTACT GTTCCAATGA CTCTCCGGAC CCCTTCTACC 
CCAGCTGTTG CGCCCCCTGT TGTCTCTACC CCCAAATTAC CTCCATCTTG TGGATCTGCT 
CTTGCAGTTT CCTCAACAGT TATCACTACA ACCGCAACTG TGTCTCCAGC CCAGCCTCTC 
CCCAAAGGGC CTCCACCTGC AACTCCTCCA CGGAAACACC GAGGGATACT CTCCACTCCC 
CCAACCATGC ACAAGCCTGT CATTGCCACC ACTCTCAGTG CCGATATTAC TCCCCAAACT 
CTGCCCAATA ACAGGGCTCC ATCTTCTGTG GCTCCTCCTG CCGTATGTTC TCATGTCTCC 
ACGGTGACTG TGTCTATGTC ACCAGGTTCT TCGGTGACCC ATTTAAGGGC CCCCTTCTCT 
ACCCCCATGC AGAAACCTAC CCCAGAACCA ATAACAAAAC CATTACCAGT GTCACAAGTA 
CAATCACCCA AAGCTCCTTC TGTGCCAACA GAGCAAACCC AGAAAATTGC CCTTGCCCCG 
GCTGTTGCCC AGGTTAAAGC ACCTTTTGTG CCAACTACTG CAAAAACTAA CGAACCATCA 
CGTCTTCCAT ATAGTGTCCC TACGGAACCA CAGATGCCAT CACAGTTAGC AGCTCCAAAA 
CTATCTGCCA AGTCCCCCTC TGTTCCAATT ATGCTGCACA TTCCCCCCTT TAAATCAGCA 
ACCCTACCTG CTTCCAGTCC CACTTCCAAA ACCATATCTT TTCCCTCTCC TCCTACAAGC 
CCAGTAACCA AACCCTTAAC ACCTACCTCC CCAGCTTATA TGGTCACTTC CTTTATATCA 
ATGCCTGTGC CTTCACCCTC TGTTGAGTCC TCCACTCCAC CTACTCCACT TACCCCCATC 
ACCCCACCTA CCCCCATCAC CCCACCTACC CCACTTACCC CCACCACCCC TCCTACCCCC 
ATTAGCCCCA CTTCCCCAAC CGCCCCATTT ACCCCCACTT CCCAAACTGT CCCTACACGG 
GTGATAGTAA GAAGTGTCAC CCCCGGGAAG GATGCACGTT ATACACCCGG AGGAAGAGTC 
ACACCCTCGG GGAGAGAAAG CACCACCCTC CGACAAGGGG TACCCCAAAA ACCTTACACT 
TTCTTGGATG AGAAAGCAAG GGGGCGGTTT GGAGTGATCC GAGAGTGTAA GGAGAATGCA 
ACTGGGAAGC ACTTCATGGC AAAGATAATC CAGTACGAAC CAGAAAACAA GCAAAACGTG 
CTGCAGGAGT ATGAGATCCT CAAGGGTCTC CACCATCAAC GAATCTTGAG TCTTCATGAA 
GCTTATATTA CCCCTCGCTA CCTTGTGTTG ATTTCTGAAA ACTGCACAGG ACGGGAACTG 
CTTTACTGCC TAGTTGAGAG GTTCCGTTAT TCTGAAGACG ACGTGGTCAA TTACCTCCTG 
CAGATCCTAC AGGGGTTGGA GTACCTCCAT GAACAAAAGG TCCTGCACCT CGATATCAAA 
CCTGAGAACG TCATTGTGTC CTACATGAAC ACTGTCAAGA TCATCGATTT GGGGAGTGCC 
CAGAACTTTA CACCGCTGGT CCTGCGACCC CTCGGCAAAC GCATAGGGAC CCTGGAATAT 
ATGTCCCCCG AAATGCTCAA AGGTGACGCA GTAGGCCCAG CAGCTGATAT TTGGGGTGTA 
GGCGTTCTGA CTTACATAAT GCTCAGCGGC CGCTCTCCGT TCTTTGAGCT GGACCCCGTG 
GAAACCGAGA ACAAGATCTT GTCGGGACGA TTTGATATCT TTAAATTGTA TTCCAACGCC 
TCCCAAAGTG CTTCCCTCTT CATTCGCAAG ATCCTGACTA TATATCCTTG GAGCCGCCCA 
TCACTTAAGG AATGTTTTTC CAATCCCTGG CTCCAGGATG CCTACCTAAT GAAGCTTAGA 
CGCCAAACCC TAACCTTCAC CACTAACAAG CTCAAAGAGT TCCTCGTGGA GCAACAGAGG 
AGACGCGCAG ACACAGCCAC TAAGCACAAA GTCCTCCTTC GTTCGTACCA CGGAAGCCAG 
CCCTCAGCTC CTGTCACTCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031748705.1
CDS	248..11371
Translation	MKKSWMKRRSQKSGHPRRVFGRFTHVFRSCRKHSYDSEPGEEDAGGPQLQRTIRAPNNTPLGTPSGDSDTLLASSLHTTPSTLTALSQTDELSDWSGSQQTVLERDPRNARQGFTQGGGARHLGVEPLIPASSNEFPGSVWGSEVSLSGFSDLYSSAFSLYRGRSCAIRVFLPEGGLRRGSINGSVTDSPRNFVNPRPPIIPPPSPRIGQRPAVIPAGRGPPTPVTPRKKTTMPNQYQDTVTEELEEKIKKPKSSVGSQASGQDSRPVTPLSETSGRVSILRASPKLVRSGSKIFEKLRCLEERRKSLDQTDSPFPVHSWLPLRKARSFDQPGAEGLGAGLGSSNEELRDDLRDGVRSEIGGFTLRYPSLRHKTTSLDDRGALYGRISDIETRFSQELTRIKRTVSQQQLVRSSQDLSGRSPSPQRSLAAQPAPVDPKLTKVSTAPKPSETEEKQPARKPLVRSFATVNQLSEIKEPLGFSSQPPTLPSKSSNGSAVYQDAVRHSETNMKSQPHLTGKIIAPKTVSPQVFKAMTLPAQSQKEAVGDTQPKRVPDVGSVPPPPSTHAPAALEIAQRGLVKEKGTVSEEKKYLPWALPVGDNNNSQMPQGKGGGQAKKHSDAHTSSKSNKSGRSKGKSRRSRPLSPELESSDDSYVSAGEDPAEAPVFEIPLQSALVNAGTEVLLKCIISGNPSPEVFWRKDHVLLKNSPTHQIRVEGERHTLLLRWALPSDSGIYTVTARNEVGEASSCGALTVKPAPTKESPAHRGTPRDLLSPITSDEEYLSPQEDLSEPTTPQHKMAGKGPAFKAPPTFKMPLLDQKGFEGQEIVLSVQVEGEPKPIINWLKNKQQVKPGGRFRISEGAWGIFSLHIAGADKRDSGFYTCKAINEYGTKQCEAKIEVLAPPGSTSLEVLAPLQDVSVCAGEAAVFECVVSGPADMDVDWMFRGKLLQPALLDCKMRFNGKQCVLLLNSVHEDDSGVYTCKLSTARDELTCSAMLTVHPSLAPLFTRKLSDRDVIEGRTARLDCKISGTPPPVVTWTHFGQPIEESENIHVLREGGHHTLLIPHVANEDEGQYTATAHNKHGEAECSAELYVEEPRSATASHISKLEKMPSIPEEPEMQECEVEGVMMPDFLRPLQDIDVVEFREVQLECQVTGLPYPTITWYHNGQKIQSSDDRRMTQYKDIHRLVFPSVTHSHAGVYKSVISNKVGKAACYAHLYVTDLVPTPPDGPPIITSVTGRIVKLQWKPPKRLDPAIDLAQITYSVQQQAVGSPQWTVITTNLKDTCYCVRSLTKEHQYLFRVTTNTPTSHSKPSPPSESVCLFNRGPYLEEMPSILDRPEILYMVENQPLCITVTLNHVHAKVTWQRIDTVLSNVEGVYELSMPDDDQHSLTLFSPRKSDLGQLTFEAKNRYGSDSCTISIELAEAPRFESIMEDIEIGVGETARFVVVVEGKPIPDIMWYKDGVLLAESGHFSFVYDDTECSLVILNTALGDSGVYTCTARNLSGEISCKAELQVCPDEPGAGSAIQEIDILKVRRLTDFYNIHKEIGRGAFSYVRHVVEKSNGRDLAAKFIPFRGATRESARRERDILSKLRHDRIIFFYDAFEKRSALIIVMELCSQEELLERLTRKPTVSESEIRSFIRQILEGLDYLHHKNILHLDIKPENILMADTTSEQIRICDFGNAQELTPGDPQYCKYGTPEFVAPEIVNQMPISTVTDIWPVGVLAYLCLTGVSPFVGENDYTTLMNIRGYTVAFEEKMFVGLTREARGFLIKVLGNEKLRPNAEESLEHPWFKTLAKGKSISTDHLKLFQSRRKWQRSLISYKSNMVMRTIPELLQDTSNHLSIAVPKNSKEASGLSSSSDSDDLEELPFVPMPLQVQFSGSRMSLNEIPTDEEQLSQPQDNGSCREDEEEGSQAVEISSTFKEQAVQQKEATVQASPLSKHAKGTLTRNRSAEVGSSSDEETSETEKREHPRRPLKKGSSLESADVPDGEIKAARRGELRRGSSADSALLLNVSTTDGDEERDRPDKGMIKAASMELPTRCRSPGRLEDRGTLRRKFGSADEEYAQRLEMMRQRLLRGGSADGKLSGLRGPLIETLSVDKKRAEQISIRSPRSDQSSLSPIPAIKLTRAASSEAAPGREATEERVLRKTSSFSHSDTEPLVLHRRSGAPLEIPLAQLEAQRLKESPSLSALTDRLESRPQTPREMIPKSLTPEAPQLKAESGEPETQSDKEAEKGTTPLIQHHKDKDSNSLKDLVIVLPRVTPPLDKMASKTELPSIQTERAQDKIPRADVNLPATTSSNETVPTKPPPLLLPSKQSLSVPPEPLPSSVLTNEMPKSKEEYSSSRAIKAKESISKTETTDQQDKIKEPLPQAVVPLPLPMPASQVLKPSTAKVQIADSPTMPENDASLPQITGKEKLSSPAVQPVDKTAAISSPYAAMIQTLQVPEEPETVVEECSKAPLKAQPVFQEIPIATGDAKAQGISQVNNKEGKDELSQEPKLINTRSSSTENVPFISNIDSEEIFEAKFKRNRENSLTRGLKKLTRTWSEEKNLAAAHASREEEMYRPSPVGVPLEFLVPAALGLHERSRSVQDLPDAERDSSFMRKLSLRFKKTPPTERKQKPAEEVEGTGRRLSWSIGRGSSKDKKDTESLNSETGSIETITDKHLKDQKKSSQSPVLAMRKKIGSTMDRLSMKLRSQSEDRRDAERTEKKEGRTDKSSPLMSLLRRSNSEGENLKKLGIPQNQLASQVSAPSTESIESGLSIKSEMVVKDDRRSRWDRWGLSRSKKEKMTSQPSIPASLMHEDGSIVGRQYIRNESDFPPVFHIKLKDIVVLEGEPVTLSCLPAGSPSPQILWKKDKMSLERGEKVHIKSCPDGRQQLIIPAAGQREAGLYECVAANALGSVASSCTVSVARIPDPPGTPEIPQKYKNTVLVLWKTHEHNSPCTYILEKQVNGDGEWKVLSSGITDCYFNVTELQEGTVRFRVACANKAGKGPYSNVSKTIVVDAEDRKPGTPSVQKARPVGAAVSTTVPMTLRTPSTPAVAPPVVSTPKLPPSCGSALAVSSTVITTTATVSPAQPLPKGPPPATPPRKHRGILSTPPTMHKPVIATTLSADITPQTLPNNRAPSSVAPPAVCSHVSTVTVSMSPGSSVTHLRAPFSTPMQKPTPEPITKPLPVSQVQSPKAPSVPTEQTQKIALAPAVAQVKAPFVPTTAKTNEPSRLPYSVPTEPQMPSQLAAPKLSAKSPSVPIMLHIPPFKSATLPASSPTSKTISFPSPPTSPVTKPLTPTSPAYMVTSFISMPVPSPSVESSTPPTPLTPITPPTPITPPTPLTPTTPPTPISPTSPTAPFTPTSQTVPTRVIVRSVTPGKDARYTPGGRVTPSGRESTTLRQGVPQKPYTFLDEKARGRFGVIRECKENATGKHFMAKIIQYEPENKQNVLQEYEILKGLHHQRILSLHEAYITPRYLVLISENCTGRELLYCLVERFRYSEDDVVNYLLQILQGLEYLHEQKVLHLDIKPENVIVSYMNTVKIIDLGSAQNFTPLVLRPLGKRIGTLEYMSPEMLKGDAVGPAADIWGVGVLTYIMLSGRSPFFELDPVETENKILSGRFDIFKLYSNASQSASLFIRKILTIYPWSRPSLKECFSNPWLQDAYLMKLRRQTLTFTTNKLKEFLVEQQRRRADTATKHKVLLRSYHGSQPSAPVTQ

Target ORF information:

RefSeq Version	XM_031892845.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis striated muscle enriched protein kinase (speg), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031892845.1

ORF Insert Sequence:

ATGAAGAAGA GTTGGATGAA AAGACGCTCC CAGAAATCGG GACACCCTCG GAGGGTCTTC 
GGACGCTTTA CTCATGTATT CCGCTCCTGC AGGAAACACA GCTACGACTC AGAACCAGGG 
GAAGAAGATG CAGGGGGCCC CCAATTACAA CGCACAATTC GGGCACCCAA CAATACGCCT 
CTGGGAACCC CTTCAGGGGA TTCAGATACG CTGCTGGCGA GCTCCCTGCA CACCACGCCC 
AGCACGCTGA CTGCACTCTC TCAAACAGAC GAGCTCTCCG ATTGGTCAGG CAGCCAACAG 
ACAGTACTAG AAAGGGACCC AAGGAATGCA AGGCAAGGCT TCACTCAGGG AGGAGGGGCT 
CGCCATCTGG GGGTGGAGCC CCTCATTCCA GCTTCCAGCA ACGAGTTCCC GGGCAGTGTC 
TGGGGGTCAG AGGTCAGCCT TTCTGGGTTC AGTGATCTGT ATAGCAGCGC CTTCAGCCTG 
TACCGAGGCA GATCTTGTGC CATCCGGGTG TTCCTTCCGG AAGGGGGTCT GCGGAGGGGT 
AGCATCAATG GTTCTGTAAC AGACTCCCCA CGAAACTTTG TCAATCCTAG GCCGCCTATC 
ATACCACCAC CCTCTCCGCG CATTGGCCAG CGTCCAGCTG TCATTCCTGC AGGCCGTGGA 
CCCCCCACCC CTGTTACACC TAGAAAGAAA ACCACAATGC CCAACCAGTA CCAGGACACA 
GTGACTGAGG AGTTAGAAGA GAAGATTAAG AAGCCAAAGT CCTCTGTTGG CTCTCAGGCT 
AGTGGCCAAG ATTCTCGCCC TGTAACTCCA CTAAGTGAGA CATCAGGGCG GGTGTCTATC 
CTTAGGGCAT CCCCAAAGCT AGTCCGCTCA GGCTCTAAGA TCTTTGAAAA GCTTAGGTGT 
CTCGAAGAGA GGAGAAAAAG TCTGGACCAG ACAGACAGCC CCTTTCCAGT GCACTCATGG 
TTGCCTCTCC GTAAGGCACG ATCCTTTGAT CAGCCGGGGG CTGAGGGGCT TGGTGCTGGT 
CTAGGTTCCT CAAACGAAGA GCTAAGGGAT GATCTTAGAG ACGGGGTAAG ATCAGAAATA 
GGTGGGTTTA CTCTGAGGTA CCCCTCTCTG AGACACAAGA CTACCTCACT GGATGACAGG 
GGAGCTCTCT ATGGACGAAT AAGTGACATT GAGACCCGCT TCTCACAAGA GCTGACTCGC 
ATCAAAAGAA CTGTGTCCCA GCAACAGCTA GTCCGTTCCT CACAGGATCT ATCTGGAAGA 
TCACCGTCAC CCCAAAGATC CCTAGCTGCC CAGCCTGCTC CTGTGGACCC TAAGCTAACC 
AAAGTGTCAA CGGCTCCAAA GCCTTCTGAA ACAGAAGAGA AGCAGCCAGC AAGGAAGCCA 
CTAGTCAGAA GCTTTGCCAC CGTCAACCAA CTGTCAGAAA TTAAGGAACC TCTGGGGTTT 
TCAAGCCAAC CCCCTACACT GCCATCAAAA TCATCCAATG GTTCTGCAGT ATATCAGGAT 
GCAGTGAGGC ACTCGGAAAC AAATATGAAA TCACAACCAC ACCTTACAGG AAAAATCATT 
GCACCAAAAA CTGTTTCCCC ACAAGTATTT AAAGCTATGA CTCTCCCCGC ACAATCCCAA 
AAAGAGGCAG TCGGCGACAC ACAGCCGAAG AGAGTTCCTG ATGTTGGTAG CGTGCCTCCG 
CCACCCAGCA CACATGCCCC GGCAGCCCTA GAGATAGCTC AGAGGGGACT TGTTAAAGAG 
AAGGGGACTG TAAGTGAAGA GAAGAAGTAT CTACCCTGGG CACTTCCTGT AGGAGACAAT 
AACAACAGCC AAATGCCACA GGGTAAGGGT GGGGGACAGG CCAAAAAGCA CAGCGATGCA 
CACACCAGCA GCAAAAGCAA CAAAAGCGGC AGAAGCAAAG GGAAGAGTCG CCGAAGCAGG 
CCATTGTCAC CAGAACTTGA GTCATCGGAT GATTCCTACG TATCGGCGGG AGAGGATCCA 
GCAGAGGCGC CTGTGTTTGA GATTCCTTTG CAAAGTGCAC TAGTAAACGC CGGCACTGAG 
GTCTTACTGA AATGTATTAT ATCTGGAAAC CCAAGTCCAG AAGTGTTTTG GAGAAAGGAT 
CACGTGTTGC TGAAAAACAG TCCGACCCAT CAAATACGAG TAGAGGGCGA GAGACACACG 
TTGCTACTGC GCTGGGCACT GCCCTCAGAC TCGGGTATCT ATACAGTGAC TGCGCGGAAT 
GAAGTTGGGG AAGCCAGCAG CTGCGGTGCC CTCACTGTAA AACCAGCACC TACCAAAGAG 
TCGCCGGCAC ATCGGGGAAC ACCTCGTGAC CTGCTCAGCC CAATAACATC GGATGAAGAG 
TATCTAAGCC CCCAGGAAGA TTTAAGTGAG CCTACCACCC CCCAACATAA GATGGCAGGC 
AAAGGCCCCG CATTCAAAGC TCCACCCACT TTCAAGATGC CTCTTTTGGA CCAGAAAGGG 
TTTGAAGGCC AAGAGATTGT TCTCAGCGTT CAGGTGGAAG GGGAACCCAA GCCTATTATC 
AACTGGCTGA AGAACAAGCA GCAGGTGAAG CCTGGTGGAA GGTTCCGCAT ATCAGAAGGT 
GCTTGGGGGA TCTTCTCGCT ACATATTGCC GGGGCTGATA AACGAGATTC CGGATTCTAC 
ACCTGTAAAG CAATAAATGA GTATGGGACA AAGCAATGTG AAGCCAAGAT AGAGGTTCTA 
GCACCCCCTG GCAGTACCAG CTTGGAGGTT CTGGCCCCCT TGCAGGATGT GAGTGTGTGC 
GCTGGGGAGG CAGCGGTGTT TGAATGCGTG GTGTCGGGCC CCGCCGATAT GGACGTGGAC 
TGGATGTTCC GGGGCAAGCT GCTCCAGCCA GCACTGCTGG ACTGTAAGAT GCGTTTCAAC 
GGAAAGCAGT GTGTGCTGCT GCTGAATTCA GTCCATGAAG ATGACAGTGG GGTCTACACC 
TGCAAACTGA GCACTGCAAG AGATGAGCTG ACCTGTAGCG CTATGCTAAC TGTGCACCCA 
TCGCTGGCGC CTCTCTTCAC TCGCAAGTTG TCAGATCGAG ATGTTATAGA GGGACGCACA 
GCTCGGCTGG ACTGTAAAAT CAGTGGCACC CCGCCCCCTG TTGTCACGTG GACTCATTTT 
GGACAGCCCA TAGAGGAAAG TGAGAACATC CACGTTTTGA GAGAAGGTGG ACATCACACC 
TTACTGATAC CCCACGTGGC GAATGAGGAC GAGGGTCAGT ACACAGCGAC TGCCCATAAC 
AAGCATGGGG AGGCCGAATG CTCTGCTGAA CTCTACGTGG AGGAGCCTCG TTCTGCTACA 
GCATCTCATA TCTCCAAGCT GGAGAAGATG CCATCTATTC CAGAAGAGCC AGAGATGCAG 
GAGTGTGAGG TAGAGGGTGT GATGATGCCG GATTTCCTGC GACCTCTGCA GGACATTGAC 
GTGGTGGAAT TTCGGGAGGT ACAACTTGAG TGTCAGGTAA CCGGTCTGCC GTACCCTACC 
ATCACCTGGT ATCACAATGG TCAGAAGATC CAGAGCTCTG ATGATCGCAG AATGACTCAA 
TATAAAGACA TCCACCGCCT GGTTTTCCCC TCTGTTACTC ATTCTCATGC CGGAGTCTAT 
AAAAGTGTCA TCTCAAACAA AGTGGGGAAA GCAGCCTGCT ATGCGCACCT GTATGTCACA 
GATTTGGTAC CCACTCCTCC AGATGGTCCC CCCATCATTA CCTCTGTAAC TGGGAGAATT 
GTGAAGCTCC AGTGGAAGCC ACCCAAGAGA TTGGACCCAG CCATTGATCT GGCTCAGATT 
ACATACTCAG TCCAGCAGCA AGCGGTGGGA TCTCCCCAAT GGACAGTCAT CACCACCAAC 
CTAAAAGACA CTTGTTATTG TGTCAGATCT CTGACCAAGG AACATCAGTA TCTTTTCCGT 
GTGACCACAA ACACCCCAAC TTCTCACAGT AAACCTTCCC CTCCCAGTGA AAGCGTTTGT 
CTCTTTAATA GAGGTCCCTA CCTGGAGGAG ATGCCTTCTA TACTGGATCG GCCTGAAATC 
TTGTATATGG TGGAAAATCA GCCTCTTTGC ATAACCGTGA CACTGAACCA CGTACATGCC 
AAAGTCACGT GGCAGAGAAT AGACACCGTT CTGAGCAATG TCGAGGGTGT GTATGAGCTG 
AGTATGCCGG ATGACGACCA GCACTCGCTC ACGTTGTTCA GCCCAAGGAA GTCTGACCTG 
GGGCAGCTGA CATTTGAGGC TAAAAACCGC TACGGATCAG ATAGCTGCAC TATTTCCATC 
GAGCTCGCAG AGGCTCCTCG CTTTGAGTCC ATCATGGAAG ACATAGAGAT CGGTGTTGGT 
GAAACAGCTC GTTTTGTGGT CGTGGTGGAA GGGAAGCCTA TTCCAGACAT CATGTGGTAC 
AAAGATGGGG TACTTTTGGC AGAGAGCGGT CACTTCAGCT TTGTGTATGA TGATACCGAG 
TGCTCTCTGG TGATCTTAAA CACGGCACTT GGTGACTCCG GTGTGTACAC CTGCACGGCA 
CGGAACCTTT CAGGGGAAAT TTCCTGCAAA GCGGAGCTGC AAGTGTGCCC AGATGAACCA 
GGGGCAGGAT CTGCAATACA GGAAATAGAT ATACTCAAAG TTCGTAGATT GACTGATTTC 
TACAACATCC ATAAAGAAAT TGGCAGAGGG GCCTTCTCCT ATGTCCGACA TGTGGTAGAA 
AAGAGCAACG GGCGAGATCT TGCTGCTAAA TTCATACCGT TCCGTGGGGC AACCAGGGAG 
AGTGCACGGA GGGAGCGGGA CATACTGTCC AAGCTCAGAC ACGACAGGAT CATCTTCTTC 
TACGATGCAT TTGAGAAAAG AAGTGCTCTA ATCATTGTCA TGGAACTCTG TTCTCAAGAG 
GAGCTCCTAG AGAGGTTGAC AAGAAAGCCT ACAGTGAGCG AATCAGAGAT CCGCTCGTTT 
ATACGGCAAA TCCTAGAAGG GTTAGATTAT TTGCACCATA AAAACATTCT GCATTTGGAC 
ATAAAGCCAG AGAACATCCT GATGGCCGAT ACGACTAGTG AACAGATTCG CATTTGTGAC 
TTTGGAAACG CTCAGGAACT AACCCCGGGG GATCCTCAGT ACTGCAAATA CGGAACCCCG 
GAATTTGTGG CGCCCGAAAT TGTGAACCAG ATGCCAATTT CTACAGTAAC GGACATATGG 
CCAGTTGGAG TCCTGGCATA TTTATGCCTT ACCGGAGTGT CGCCCTTTGT TGGGGAGAAT 
GATTACACCA CTCTGATGAA TATCCGGGGT TACACTGTGG CATTTGAAGA GAAGATGTTT 
GTTGGTCTCA CACGAGAAGC ACGTGGTTTC CTAATCAAAG TCTTGGGCAA TGAGAAGCTG 
AGGCCTAATG CTGAAGAGAG TTTGGAGCAC CCCTGGTTCA AAACTTTAGC GAAAGGGAAA 
AGCATCAGCA CAGACCATCT GAAGCTGTTC CAGTCTCGCC GTAAATGGCA GCGCTCACTT 
ATCAGCTATA AGTCCAATAT GGTGATGAGA ACCATTCCAG AGCTGCTTCA GGACACAAGC 
AATCACTTGT CCATTGCTGT ACCCAAGAAT TCCAAGGAAG CATCCGGATT GTCATCCTCC 
TCTGATTCTG ATGACCTGGA AGAGCTTCCC TTTGTCCCTA TGCCTCTCCA GGTCCAATTC 
TCTGGATCAC GCATGTCCTT GAATGAGATC CCAACAGATG AGGAACAACT GTCTCAGCCC 
CAGGACAATG GCAGCTGTAG AGAAGATGAG GAAGAAGGAA GTCAGGCAGT GGAAATTAGT 
AGCACATTTA AAGAACAAGC AGTTCAGCAG AAAGAAGCAA CAGTCCAAGC AAGTCCATTG 
TCCAAGCATG CCAAAGGAAC CCTTACAAGG AACAGGTCTG CTGAAGTTGG TTCTTCTTCT 
GATGAGGAAA CTTCAGAAAC TGAAAAAAGA GAGCATCCTC GTAGGCCTCT GAAAAAAGGT 
TCCAGCTTAG AATCTGCCGA CGTTCCTGAT GGGGAAATTA AAGCAGCTCG TAGAGGAGAG 
CTGAGGAGGG GAAGCTCTGC AGATAGTGCT CTTTTGCTCA ATGTGTCTAC AACAGATGGA 
GATGAAGAGA GAGATCGACC AGACAAGGGA ATGATAAAAG CTGCCTCAAT GGAGCTACCA 
ACCAGATGCA GGAGCCCAGG TAGATTGGAG GATCGAGGCA CACTACGAAG AAAGTTTGGA 
TCAGCTGATG AAGAATATGC TCAGCGGTTA GAAATGATGA GACAGAGGCT ATTGAGGGGA 
GGTTCGGCAG ATGGAAAACT CAGTGGTCTT AGAGGCCCAC TCATAGAAAC ACTTTCTGTA 
GACAAGAAGC GAGCAGAGCA GATATCTATA CGCTCCCCAA GAAGTGATCA GTCTTCTCTG 
AGTCCTATTC CCGCCATCAA ACTAACCCGA GCTGCTTCCA GTGAGGCTGC GCCTGGTAGA 
GAAGCAACTG AAGAAAGAGT CTTAAGGAAA ACCAGTTCCT TTTCTCACAG CGACACAGAG 
CCACTGGTCT TGCATCGCAG GTCAGGGGCA CCCCTAGAAA TACCTCTTGC TCAGTTAGAG 
GCACAGCGTC TTAAAGAGTC TCCCTCTCTT TCAGCATTGA CAGACCGATT AGAATCACGT 
CCTCAAACAC CGCGAGAGAT GATTCCCAAA TCTCTAACTC CAGAAGCACC GCAATTGAAG 
GCAGAAAGTG GGGAGCCAGA AACTCAGAGT GATAAGGAAG CTGAGAAGGG CACCACACCT 
CTTATTCAGC ATCATAAAGA CAAAGACTCA AATTCATTGA AGGACTTGGT TATTGTTCTT 
CCAAGAGTGA CTCCACCTTT GGATAAAATG GCATCTAAAA CAGAGTTACC AAGTATTCAA 
ACAGAGAGGG CACAGGACAA AATCCCTAGA GCAGATGTGA ACCTGCCAGC TACTACTTCA 
TCAAATGAAA CTGTGCCAAC AAAGCCACCA CCCTTACTTT TGCCTTCTAA GCAGTCTCTT 
TCTGTGCCAC CTGAACCTTT ACCCTCTTCA GTACTGACAA ATGAGATGCC TAAATCTAAA 
GAGGAGTACT CGTCATCAAG GGCCATTAAG GCCAAGGAAT CCATATCAAA AACAGAAACA 
ACAGACCAGC AAGATAAAAT TAAAGAACCT TTACCACAAG CTGTTGTTCC ATTACCATTG 
CCTATGCCAG CCTCCCAAGT GCTAAAACCT TCCACTGCAA AAGTACAGAT AGCAGATTCT 
CCAACAATGC CTGAGAATGA TGCATCACTT CCACAGATAA CAGGTAAAGA GAAACTATCT 
TCTCCAGCTG TCCAACCAGT AGATAAAACA GCAGCTATAT CATCACCTTA TGCTGCTATG 
ATTCAAACAC TTCAAGTGCC CGAGGAACCT GAGACAGTTG TTGAGGAATG TTCAAAGGCC 
CCATTAAAAG CTCAGCCAGT ATTTCAAGAA ATTCCTATAG CCACTGGAGA TGCAAAGGCT 
CAAGGAATAT CACAAGTAAA CAATAAAGAA GGCAAGGATG AGCTATCACA AGAACCCAAG 
CTGATTAACA CCAGATCTTC CAGCACAGAG AATGTCCCAT TTATCAGCAA CATTGATTCA 
GAAGAAATCT TTGAGGCTAA GTTCAAGAGG AACCGTGAAA ATTCTCTTAC CCGAGGTCTA 
AAAAAGCTTA CCAGGACTTG GTCAGAGGAG AAGAATTTAG CTGCAGCCCA TGCATCTCGG 
GAAGAAGAAA TGTACAGGCC TAGTCCTGTG GGTGTCCCAC TAGAGTTCCT TGTTCCTGCA 
GCTCTTGGGC TACATGAAAG ATCAAGATCA GTGCAAGATC TGCCAGATGC TGAGAGGGAT 
TCAAGCTTTA TGAGAAAGCT TTCTTTGAGA TTTAAGAAAA CTCCACCAAC TGAAAGGAAG 
CAGAAACCAG CAGAAGAAGT AGAAGGTACT GGTAGACGAC TTTCATGGTC AATAGGTAGA 
GGAAGTTCCA AAGATAAGAA GGATACAGAG AGCCTGAACT CTGAAACAGG CTCAATAGAA 
ACCATCACAG ACAAACATCT AAAGGACCAG AAAAAGAGTA GTCAATCACC AGTGTTGGCC 
ATGAGAAAGA AAATAGGCAG TACTATGGAT CGACTTTCTA TGAAACTAAG GAGCCAATCA 
GAAGATAGAA GGGATGCAGA GAGAACAGAG AAGAAAGAAG GGCGTACAGA CAAAAGTTCT 
CCATTAATGT CTTTACTTAG GAGATCCAAC TCAGAAGGAG AGAACTTAAA GAAACTAGGT 
ATTCCCCAGA ACCAACTTGC TTCCCAGGTT TCAGCTCCAT CAACTGAGTC TATTGAATCT 
GGTCTCAGCA TCAAATCTGA AATGGTAGTC AAGGATGATA GGCGCTCAAG GTGGGATCGT 
TGGGGCCTGT CACGTAGCAA AAAGGAGAAG ATGACATCGC AGCCAAGTAT TCCAGCCAGT 
CTGATGCATG AAGATGGCAG TATTGTTGGC AGACAATACA TTCGTAATGA GTCAGACTTC 
CCCCCAGTCT TCCATATCAA GCTGAAGGAC ATCGTGGTGC TAGAGGGAGA GCCGGTGACT 
CTGTCCTGCT TACCTGCTGG CAGCCCTTCC CCCCAGATCC TGTGGAAGAA AGATAAGATG 
TCCTTGGAAA GAGGAGAAAA GGTGCATATA AAATCCTGCC CCGATGGCAG ACAGCAGCTC 
ATTATTCCCG CCGCCGGGCA GAGGGAAGCC GGTCTGTATG AATGCGTAGC AGCCAATGCC 
CTGGGCTCAG TGGCCAGCTC CTGCACAGTG TCTGTGGCAA GAATACCTGA TCCCCCGGGG 
ACCCCAGAGA TCCCACAGAA GTATAAGAAC ACGGTGCTGG TTCTATGGAA GACCCACGAG 
CACAACTCCC CTTGCACCTA CATCCTGGAG AAACAGGTTA ATGGTGATGG GGAATGGAAG 
GTTCTAAGTT CTGGAATCAC CGACTGTTAC TTCAATGTGA CGGAGCTCCA GGAAGGCACT 
GTCAGGTTCC GCGTCGCTTG TGCCAACAAA GCAGGAAAGG GGCCCTACAG CAATGTGTCC 
AAGACAATTG TCGTAGATGC AGAAGATCGT AAGCCAGGAA CACCCTCTGT CCAGAAGGCA 
CGGCCCGTGG GAGCTGCAGT GTCCACTACT GTTCCAATGA CTCTCCGGAC CCCTTCTACC 
CCAGCTGTTG CGCCCCCTGT TGTCTCTACC CCCAAATTAC CTCCATCTTG TGGATCTGCT 
CTTGCAGTTT CCTCAACAGT TATCACTACA ACCGCAACTG TGTCTCCAGC CCAGCCTCTC 
CCCAAAGGGC CTCCACCTGC AACTCCTCCA CGGAAACACC GAGGGATACT CTCCACTCCC 
CCAACCATGC ACAAGCCTGT CATTGCCACC ACTCTCAGTG CCGATATTAC TCCCCAAACT 
CTGCCCAATA ACAGGGCTCC ATCTTCTGTG GCTCCTCCTG CCGTATGTTC TCATGTCTCC 
ACGGTGACTG TGTCTATGTC ACCAGGTTCT TCGGTGACCC ATTTAAGGGC CCCCTTCTCT 
ACCCCCATGC AGAAACCTAC CCCAGAACCA ATAACAAAAC CATTACCAGT GTCACAAGTA 
CAATCACCCA AAGCTCCTTC TGTGCCAACA GAGCAAACCC AGAAAATTGC CCTTGCCCCG 
GCTGTTGCCC AGGTTAAAGC ACCTTTTGTG CCAACTACTG CAAAAACTAA CGAACCATCA 
CGTCTTCCAT ATAGTGTCCC TACGGAACCA CAGATGCCAT CACAGTTAGC AGCTCCAAAA 
CTATCTGCCA AGTCCCCCTC TGTTCCAATT ATGCTGCACA TTCCCCCCTT TAAATCAGCA 
ACCCTACCTG CTTCCAGTCC CACTTCCAAA ACCATATCTT TTCCCTCTCC TCCTACAAGC 
CCAGTAACCA AACCCTTAAC ACCTACCTCC CCAGCTTATA TGGTCACTTC CTTTATATCA 
ATGCCTGTGC CTTCACCCTC TGTTGAGTCC TCCACTCCAC CTACTCCACT TACCCCCATC 
ACCCCACCTA CCCCCATCAC CCCACCTACC CCACTTACCC CCACCACCCC TCCTACCCCC 
ATTAGCCCCA CTTCCCCAAC CGCCCCATTT ACCCCCACTT CCCAAACTGT CCCTACACGG 
GTGATAGTAA GAAGTGTCAC CCCCGGGAAG GATGCACGTT ATACACCCGG AGGAAGAGTC 
ACACCCTCGG GGAGAGAAAG CACCACCCTC CGACAAGGGG TACCCCAAAA ACCTTACACT 
TTCTTGGATG AGAAAGCAAG GGGGCGGTTT GGAGTGATCC GAGAGTGTAA GGAGAATGCA 
ACTGGGAAGC ACTTCATGGC AAAGATAATC CAGTACGAAC CAGAAAACAA GCAAAACGTG 
CTGCAGGAGT ATGAGATCCT CAAGGGTCTC CACCATCAAC GAATCTTGAG TCTTCATGAA 
GCTTATATTA CCCCTCGCTA CCTTGTGTTG ATTTCTGAAA ACTGCACAGG ACGGGAACTG 
CTTTACTGCC TAGTTGAGAG GTTCCGTTAT TCTGAAGACG ACGTGGTCAA TTACCTCCTG 
CAGATCCTAC AGGGGTTGGA GTACCTCCAT GAACAAAAGG TCCTGCACCT CGATATCAAA 
CCTGAGAACG TCATTGTGTC CTACATGAAC ACTGTCAAGA TCATCGATTT GGGGAGTGCC 
CAGAACTTTA CACCGCTGGT CCTGCGACCC CTCGGCAAAC GCATAGGGAC CCTGGAATAT 
ATGTCCCCCG AAATGCTCAA AGGTGACGCA GTAGGCCCAG CAGCTGATAT TTGGGGTGTA 
GGCGTTCTGA CTTACATAAT GCTCAGCGGC CGCTCTCCGT TCTTTGAGCT GGACCCCGTG 
GAAACCGAGA ACAAGATCTT GTCGGGACGA TTTGATATCT TTAAATTGTA TTCCAACGCC 
TCCCAAAGTG CTTCCCTCTT CATTCGCAAG ATCCTGACTA TATATCCTTG GAGCCGCCCA 
TCACTTAAGG AATGTTTTTC CAATCCCTGG CTCCAGGATG CCTACCTAAT GAAGCTTAGA 
CGCCAAACCC TAACCTTCAC CACTAACAAG CTCAAAGAGT TCCTCGTGGA GCAACAGAGG 
AGACGCGCAG ACACAGCCAC TAAGCACAAA GTCCTCCTTC GTTCGTACCA CGGAAGCCAG 
CCCTCAGCTC CTGTCACTCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

speg ( XM_031892847.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031748707.1 Xenopus tropicalis striated muscle enriched protein kinase (speg), transcript variant X4, mRNA.

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CloneID	OXa66225
Clone ID Related Accession (Same CDS sequence)	XM_031892847.1
Accession Version	XM_031892847.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10428bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	striated muscle preferentially expressed protein kinase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAACC AGTACCAGGA CACAGTGACT GAGGAGTTAG AAGAGAAGAT TAAGAAGCCA 
AAGTCCTCTG TTGGCTCTCA GGCTAGTGGC CAAGATTCTC GCCCTGTAAC TCCACTAAGT 
GAGACATCAG GGCGGGTGTC TATCCTTAGG GCATCCCCAA AGCTAGTCCG CTCAGGCTCT 
AAGATCTTTG AAAAGCTTAG GTGTCTCGAA GAGAGGAGAA AAAGTCTGGA CCAGACAGAC 
AGCCCCTTTC CAGTGCACTC ATGGTTGCCT CTCCGTAAGG CACGATCCTT TGATCAGCCG 
GGGGCTGAGG GGCTTGGTGC TGGTCTAGGT TCCTCAAACG AAGAGCTAAG GGATGATCTT 
AGAGACGGGG TAAGATCAGA AATAGGTGGG TTTACTCTGA GGTACCCCTC TCTGAGACAC 
AAGACTACCT CACTGGATGA CAGGGGAGCT CTCTATGGAC GAATAAGTGA CATTGAGACC 
CGCTTCTCAC AAGAGCTGAC TCGCATCAAA AGAACTGTGT CCCAGCAACA GCTAGTCCGT 
TCCTCACAGG ATCTATCTGG AAGATCACCG TCACCCCAAA GATCCCTAGC TGCCCAGCCT 
GCTCCTGTGG ACCCTAAGCT AACCAAAGTG TCAACGGCTC CAAAGCCTTC TGAAACAGAA 
GAGAAGCAGC CAGCAAGGAA GCCACTAGTC AGAAGCTTTG CCACCGTCAA CCAACTGTCA 
GAAATTAAGG AACCTCTGGG GTTTTCAAGC CAACCCCCTA CACTGCCATC AAAATCATCC 
AATGGTTCTG CAGTATATCA GGATGCAGTG AGGCACTCGG AAACAAATAT GAAATCACAA 
CCACACCTTA CAGGAAAAAT CATTGCACCA AAAACTGTTT CCCCACAAGT ATTTAAAGCT 
ATGACTCTCC CCGCACAATC CCAAAAAGAG GCAGTCGGCG ACACACAGCC GAAGAGAGTT 
CCTGATGTTG GTAGCGTGCC TCCGCCACCC AGCACACATG CCCCGGCAGC CCTAGAGATA 
GCTCAGAGGG GACTTGTTAA AGAGAAGGGG ACTGTAAGTG AAGAGAAGAA GTATCTACCC 
TGGGCACTTC CTGTAGGAGA CAATAACAAC AGCCAAATGC CACAGGGTAA GGGTGGGGGA 
CAGGCCAAAA AGCACAGCGA TGCACACACC AGCAGCAAAA GCAACAAAAG CGGCAGAAGC 
AAAGGGAAGA GTCGCCGAAG CAGGCCATTG TCACCAGAAC TTGAGTCATC GGATGATTCC 
TACGTATCGG CGGGAGAGGA TCCAGCAGAG GCGCCTGTGT TTGAGATTCC TTTGCAAAGT 
GCACTAGTAA ACGCCGGCAC TGAGGTCTTA CTGAAATGTA TTATATCTGG AAACCCAAGT 
CCAGAAGTGT TTTGGAGAAA GGATCACGTG TTGCTGAAAA ACAGTCCGAC CCATCAAATA 
CGAGTAGAGG GCGAGAGACA CACGTTGCTA CTGCGCTGGG CACTGCCCTC AGACTCGGGT 
ATCTATACAG TGACTGCGCG GAATGAAGTT GGGGAAGCCA GCAGCTGCGG TGCCCTCACT 
GTAAAACCAG CACCTACCAA AGAGTCGCCG GCACATCGGG GAACACCTCG TGACCTGCTC 
AGCCCAATAA CATCGGATGA AGAGTATCTA AGCCCCCAGG AAGATTTAAG TGAGCCTACC 
ACCCCCCAAC ATAAGATGGC AGGCAAAGGC CCCGCATTCA AAGCTCCACC CACTTTCAAG 
ATGCCTCTTT TGGACCAGAA AGGGTTTGAA GGCCAAGAGA TTGTTCTCAG CGTTCAGGTG 
GAAGGGGAAC CCAAGCCTAT TATCAACTGG CTGAAGAACA AGCAGCAGGT GAAGCCTGGT 
GGAAGGTTCC GCATATCAGA AGGTGCTTGG GGGATCTTCT CGCTACATAT TGCCGGGGCT 
GATAAACGAG ATTCCGGATT CTACACCTGT AAAGCAATAA ATGAGTATGG GACAAAGCAA 
TGTGAAGCCA AGATAGAGGT TCTAGCACCC CCTGGCAGTA CCAGCTTGGA GGTTCTGGCC 
CCCTTGCAGG ATGTGAGTGT GTGCGCTGGG GAGGCAGCGG TGTTTGAATG CGTGGTGTCG 
GGCCCCGCCG ATATGGACGT GGACTGGATG TTCCGGGGCA AGCTGCTCCA GCCAGCACTG 
CTGGACTGTA AGATGCGTTT CAACGGAAAG CAGTGTGTGC TGCTGCTGAA TTCAGTCCAT 
GAAGATGACA GTGGGGTCTA CACCTGCAAA CTGAGCACTG CAAGAGATGA GCTGACCTGT 
AGCGCTATGC TAACTGTGCA CCCATCGCTG GCGCCTCTCT TCACTCGCAA GTTGTCAGAT 
CGAGATGTTA TAGAGGGACG CACAGCTCGG CTGGACTGTA AAATCAGTGG CACCCCGCCC 
CCTGTTGTCA CGTGGACTCA TTTTGGACAG CCCATAGAGG AAAGTGAGAA CATCCACGTT 
TTGAGAGAAG GTGGACATCA CACCTTACTG ATACCCCACG TGGCGAATGA GGACGAGGGT 
CAGTACACAG CGACTGCCCA TAACAAGCAT GGGGAGGCCG AATGCTCTGC TGAACTCTAC 
GTGGAGGAGC CTCGTTCTGC TACAGCATCT CATATCTCCA AGCTGGAGAA GATGCCATCT 
ATTCCAGAAG AGCCAGAGAT GCAGGAGTGT GAGGTAGAGG GTGTGATGAT GCCGGATTTC 
CTGCGACCTC TGCAGGACAT TGACGTGGTG GAATTTCGGG AGGTACAACT TGAGTGTCAG 
GTAACCGGTC TGCCGTACCC TACCATCACC TGGTATCACA ATGGTCAGAA GATCCAGAGC 
TCTGATGATC GCAGAATGAC TCAATATAAA GACATCCACC GCCTGGTTTT CCCCTCTGTT 
ACTCATTCTC ATGCCGGAGT CTATAAAAGT GTCATCTCAA ACAAAGTGGG GAAAGCAGCC 
TGCTATGCGC ACCTGTATGT CACAGATTTG GTACCCACTC CTCCAGATGG TCCCCCCATC 
ATTACCTCTG TAACTGGGAG AATTGTGAAG CTCCAGTGGA AGCCACCCAA GAGATTGGAC 
CCAGCCATTG ATCTGGCTCA GATTACATAC TCAGTCCAGC AGCAAGCGGT GGGATCTCCC 
CAATGGACAG TCATCACCAC CAACCTAAAA GACACTTGTT ATTGTGTCAG ATCTCTGACC 
AAGGAACATC AGTATCTTTT CCGTGTGACC ACAAACACCC CAACTTCTCA CAGTAAACCT 
TCCCCTCCCA GTGAAAGCGT TTGTCTCTTT AATAGAGGTC CCTACCTGGA GGAGATGCCT 
TCTATACTGG ATCGGCCTGA AATCTTGTAT ATGGTGGAAA ATCAGCCTCT TTGCATAACC 
GTGACACTGA ACCACGTACA TGCCAAAGTC ACGTGGCAGA GAATAGACAC CGTTCTGAGC 
AATGTCGAGG GTGTGTATGA GCTGAGTATG CCGGATGACG ACCAGCACTC GCTCACGTTG 
TTCAGCCCAA GGAAGTCTGA CCTGGGGCAG CTGACATTTG AGGCTAAAAA CCGCTACGGA 
TCAGATAGCT GCACTATTTC CATCGAGCTC GCAGAGGCTC CTCGCTTTGA GTCCATCATG 
GAAGACATAG AGATCGGTGT TGGTGAAACA GCTCGTTTTG TGGTCGTGGT GGAAGGGAAG 
CCTATTCCAG ACATCATGTG GTACAAAGAT GGGGTACTTT TGGCAGAGAG CGGTCACTTC 
AGCTTTGTGT ATGATGATAC CGAGTGCTCT CTGGTGATCT TAAACACGGC ACTTGGTGAC 
TCCGGTGTGT ACACCTGCAC GGCACGGAAC CTTTCAGGGG AAATTTCCTG CAAAGCGGAG 
CTGCAAGTGT GCCCAGATGA ACCAGGGGCA GGATCTGCAA TACAGGAAAT AGATATACTC 
AAAGTTCGTA GATTGACTGA TTTCTACAAC ATCCATAAAG AAATTGGCAG AGGGGCCTTC 
TCCTATGTCC GACATGTGGT AGAAAAGAGC AACGGGCGAG ATCTTGCTGC TAAATTCATA 
CCGTTCCGTG GGGCAACCAG GGAGAGTGCA CGGAGGGAGC GGGACATACT GTCCAAGCTC 
AGACACGACA GGATCATCTT CTTCTACGAT GCATTTGAGA AAAGAAGTGC TCTAATCATT 
GTCATGGAAC TCTGTTCTCA AGAGGAGCTC CTAGAGAGGT TGACAAGAAA GCCTACAGTG 
AGCGAATCAG AGATCCGCTC GTTTATACGG CAAATCCTAG AAGGGTTAGA TTATTTGCAC 
CATAAAAACA TTCTGCATTT GGACATAAAG CCAGAGAACA TCCTGATGGC CGATACGACT 
AGTGAACAGA TTCGCATTTG TGACTTTGGA AACGCTCAGG AACTAACCCC GGGGGATCCT 
CAGTACTGCA AATACGGAAC CCCGGAATTT GTGGCGCCCG AAATTGTGAA CCAGATGCCA 
ATTTCTACAG TAACGGACAT ATGGCCAGTT GGAGTCCTGG CATATTTATG CCTTACCGGA 
GTGTCGCCCT TTGTTGGGGA GAATGATTAC ACCACTCTGA TGAATATCCG GGGTTACACT 
GTGGCATTTG AAGAGAAGAT GTTTGTTGGT CTCACACGAG AAGCACGTGG TTTCCTAATC 
AAAGTCTTGG GCAATGAGAA GCTGAGGCCT AATGCTGAAG AGAGTTTGGA GCACCCCTGG 
TTCAAAACTT TAGCGAAAGG GAAAAGCATC AGCACAGACC ATCTGAAGCT GTTCCAGTCT 
CGCCGTAAAT GGCAGCGCTC ACTTATCAGC TATAAGTCCA ATATGGTGAT GAGAACCATT 
CCAGAGCTGC TTCAGGACAC AAGCAATCAC TTGTCCATTG CTGTACCCAA GAATTCCAAG 
GAAGCATCCG GATTGTCATC CTCCTCTGAT TCTGATGACC TGGAAGAGCT TCCCTTTGTC 
CCTATGCCTC TCCAGGTCCA ATTCTCTGGA TCACGCATGT CCTTGAATGA GATCCCAACA 
GATGAGGAAC AACTGTCTCA GCCCCAGGAC AATGGCAGCT GTAGAGAAGA TGAGGAAGAA 
GGAAGTCAGG CAGTGGAAAT TAGTAGCACA TTTAAAGAAC AAGCAGTTCA GCAGAAAGAA 
GCAACAGTCC AAGCAAGTCC ATTGTCCAAG CATGCCAAAG GAACCCTTAC AAGGAACAGG 
TCTGCTGAAG TTGGTTCTTC TTCTGATGAG GAAACTTCAG AAACTGAAAA AAGAGAGCAT 
CCTCGTAGGC CTCTGAAAAA AGGTTCCAGC TTAGAATCTG CCGACGTTCC TGATGGGGAA 
ATTAAAGCAG CTCGTAGAGG AGAGCTGAGG AGGGGAAGCT CTGCAGATAG TGCTCTTTTG 
CTCAATGTGT CTACAACAGA TGGAGATGAA GAGAGAGATC GACCAGACAA GGGAATGATA 
AAAGCTGCCT CAATGGAGCT ACCAACCAGA TGCAGGAGCC CAGGTAGATT GGAGGATCGA 
GGCACACTAC GAAGAAAGTT TGGATCAGCT GATGAAGAAT ATGCTCAGCG GTTAGAAATG 
ATGAGACAGA GGCTATTGAG GGGAGGTTCG GCAGATGGAA AACTCAGTGG TCTTAGAGGC 
CCACTCATAG AAACACTTTC TGTAGACAAG AAGCGAGCAG AGCAGATATC TATACGCTCC 
CCAAGAAGTG ATCAGTCTTC TCTGAGTCCT ATTCCCGCCA TCAAACTAAC CCGAGCTGCT 
TCCAGTGAGG CTGCGCCTGG TAGAGAAGCA ACTGAAGAAA GAGTCTTAAG GAAAACCAGT 
TCCTTTTCTC ACAGCGACAC AGAGCCACTG GTCTTGCATC GCAGGTCAGG GGCACCCCTA 
GAAATACCTC TTGCTCAGTT AGAGGCACAG CGTCTTAAAG AGTCTCCCTC TCTTTCAGCA 
TTGACAGACC GATTAGAATC ACGTCCTCAA ACACCGCGAG AGATGATTCC CAAATCTCTA 
ACTCCAGAAG CACCGCAATT GAAGGCAGAA AGTGGGGAGC CAGAAACTCA GAGTGATAAG 
GAAGCTGAGA AGGGCACCAC ACCTCTTATT CAGCATCATA AAGACAAAGA CTCAAATTCA 
TTGAAGGACT TGGTTATTGT TCTTCCAAGA GTGACTCCAC CTTTGGATAA AATGGCATCT 
AAAACAGAGT TACCAAGTAT TCAAACAGAG AGGGCACAGG ACAAAATCCC TAGAGCAGAT 
GTGAACCTGC CAGCTACTAC TTCATCAAAT GAAACTGTGC CAACAAAGCC ACCACCCTTA 
CTTTTGCCTT CTAAGCAGTC TCTTTCTGTG CCACCTGAAC CTTTACCCTC TTCAGTACTG 
ACAAATGAGA TGCCTAAATC TAAAGAGGAG TACTCGTCAT CAAGGGCCAT TAAGGCCAAG 
GAATCCATAT CAAAAACAGA AACAACAGAC CAGCAAGATA AAATTAAAGA ACCTTTACCA 
CAAGCTGTTG TTCCATTACC ATTGCCTATG CCAGCCTCCC AAGTGCTAAA ACCTTCCACT 
GCAAAAGTAC AGATAGCAGA TTCTCCAACA ATGCCTGAGA ATGATGCATC ACTTCCACAG 
ATAACAGGTA AAGAGAAACT ATCTTCTCCA GCTGTCCAAC CAGTAGATAA AACAGCAGCT 
ATATCATCAC CTTATGCTGC TATGATTCAA ACACTTCAAG TGCCCGAGGA ACCTGAGACA 
GTTGTTGAGG AATGTTCAAA GGCCCCATTA AAAGCTCAGC CAGTATTTCA AGAAATTCCT 
ATAGCCACTG GAGATGCAAA GGCTCAAGGA ATATCACAAG TAAACAATAA AGAAGGCAAG 
GATGAGCTAT CACAAGAACC CAAGCTGATT AACACCAGAT CTTCCAGCAC AGAGAATGTC 
CCATTTATCA GCAACATTGA TTCAGAAGAA ATCTTTGAGG CTAAGTTCAA GAGGAACCGT 
GAAAATTCTC TTACCCGAGG TCTAAAAAAG CTTACCAGGA CTTGGTCAGA GGAGAAGAAT 
TTAGCTGCAG CCCATGCATC TCGGGAAGAA GAAATGTACA GGCCTAGTCC TGTGGGTGTC 
CCACTAGAGT TCCTTGTTCC TGCAGCTCTT GGGCTACATG AAAGATCAAG ATCAGTGCAA 
GATCTGCCAG ATGCTGAGAG GGATTCAAGC TTTATGAGAA AGCTTTCTTT GAGATTTAAG 
AAAACTCCAC CAACTGAAAG GAAGCAGAAA CCAGCAGAAG AAGTAGAAGG TACTGGTAGA 
CGACTTTCAT GGTCAATAGG TAGAGGAAGT TCCAAAGATA AGAAGGATAC AGAGAGCCTG 
AACTCTGAAA CAGGCTCAAT AGAAACCATC ACAGACAAAC ATCTAAAGGA CCAGAAAAAG 
AGTAGTCAAT CACCAGTGTT GGCCATGAGA AAGAAAATAG GCAGTACTAT GGATCGACTT 
TCTATGAAAC TAAGGAGCCA ATCAGAAGAT AGAAGGGATG CAGAGAGAAC AGAGAAGAAA 
GAAGGGCGTA CAGACAAAAG TTCTCCATTA ATGTCTTTAC TTAGGAGATC CAACTCAGAA 
GGAGAGAACT TAAAGAAACT AGGTATTCCC CAGAACCAAC TTGCTTCCCA GGTTTCAGCT 
CCATCAACTG AGTCTATTGA ATCTGGTCTC AGCATCAAAT CTGAAATGGT AGTCAAGGAT 
GATAGGCGCT CAAGGTGGGA TCGTTGGGGC CTGTCACGTA GCAAAAAGGA GAAGATGACA 
TCGCAGCCAA GTATTCCAGC CAGTCTGATG CATGAAGATG GCAGTATTGT TGGCAGACAA 
TACATTCGTA ATGAGTCAGA CTTCCCCCCA GTCTTCCATA TCAAGCTGAA GGACATCGTG 
GTGCTAGAGG GAGAGCCGGT GACTCTGTCC TGCTTACCTG CTGGCAGCCC TTCCCCCCAG 
ATCCTGTGGA AGAAAGATAA GATGTCCTTG GAAAGAGGAG AAAAGGTGCA TATAAAATCC 
TGCCCCGATG GCAGACAGCA GCTCATTATT CCCGCCGCCG GGCAGAGGGA AGCCGGTCTG 
TATGAATGCG TAGCAGCCAA TGCCCTGGGC TCAGTGGCCA GCTCCTGCAC AGTGTCTGTG 
GCAAGAATAC CTGATCCCCC GGGGACCCCA GAGATCCCAC AGAAGTATAA GAACACGGTG 
CTGGTTCTAT GGAAGACCCA CGAGCACAAC TCCCCTTGCA CCTACATCCT GGAGAAACAG 
GTTAATGGTG ATGGGGAATG GAAGGTTCTA AGTTCTGGAA TCACCGACTG TTACTTCAAT 
GTGACGGAGC TCCAGGAAGG CACTGTCAGG TTCCGCGTCG CTTGTGCCAA CAAAGCAGGA 
AAGGGGCCCT ACAGCAATGT GTCCAAGACA ATTGTCGTAG ATGCAGAAGA TCGTAAGCCA 
GGAACACCCT CTGTCCAGAA GGCACGGCCC GTGGGAGCTG CAGTGTCCAC TACTGTTCCA 
ATGACTCTCC GGACCCCTTC TACCCCAGCT GTTGCGCCCC CTGTTGTCTC TACCCCCAAA 
TTACCTCCAT CTTGTGGATC TGCTCTTGCA GTTTCCTCAA CAGTTATCAC TACAACCGCA 
ACTGTGTCTC CAGCCCAGCC TCTCCCCAAA GGGCCTCCAC CTGCAACTCC TCCACGGAAA 
CACCGAGGGA TACTCTCCAC TCCCCCAACC ATGCACAAGC CTGTCATTGC CACCACTCTC 
AGTGCCGATA TTACTCCCCA AACTCTGCCC AATAACAGGG CTCCATCTTC TGTGGCTCCT 
CCTGCCGTAT GTTCTCATGT CTCCACGGTG ACTGTGTCTA TGTCACCAGG TTCTTCGGTG 
ACCCATTTAA GGGCCCCCTT CTCTACCCCC ATGCAGAAAC CTACCCCAGA ACCAATAACA 
AAACCATTAC CAGTGTCACA AGTACAATCA CCCAAAGCTC CTTCTGTGCC AACAGAGCAA 
ACCCAGAAAA TTGCCCTTGC CCCGGCTGTT GCCCAGGTTA AAGCACCTTT TGTGCCAACT 
ACTGCAAAAA CTAACGAACC ATCACGTCTT CCATATAGTG TCCCTACGGA ACCACAGATG 
CCATCACAGT TAGCAGCTCC AAAACTATCT GCCAAGTCCC CCTCTGTTCC AATTATGCTG 
CACATTCCCC CCTTTAAATC AGCAACCCTA CCTGCTTCCA GTCCCACTTC CAAAACCATA 
TCTTTTCCCT CTCCTCCTAC AAGCCCAGTA ACCAAACCCT TAACACCTAC CTCCCCAGCT 
TATATGGTCA CTTCCTTTAT ATCAATGCCT GTGCCTTCAC CCTCTGTTGA GTCCTCCACT 
CCACCTACTC CACTTACCCC CATCACCCCA CCTACCCCCA TCACCCCACC TACCCCACTT 
ACCCCCACCA CCCCTCCTAC CCCCATTAGC CCCACTTCCC CAACCGCCCC ATTTACCCCC 
ACTTCCCAAA CTGTCCCTAC ACGGGTGATA GTAAGAAGTG TCACCCCCGG GAAGGATGCA 
CGTTATACAC CCGGAGGAAG AGTCACACCC TCGGGGAGAG AAAGCACCAC CCTCCGACAA 
GGGGTACCCC AAAAACCTTA CACTTTCTTG GATGAGAAAG CAAGGGGGCG GTTTGGAGTG 
ATCCGAGAGT GTAAGGAGAA TGCAACTGGG AAGCACTTCA TGGCAAAGAT AATCCAGTAC 
GAACCAGAAA ACAAGCAAAA CGTGCTGCAG GAGTATGAGA TCCTCAAGGG TCTCCACCAT 
CAACGAATCT TGAGTCTTCA TGAAGCTTAT ATTACCCCTC GCTACCTTGT GTTGATTTCT 
GAAAACTGCA CAGGACGGGA ACTGCTTTAC TGCCTAGTTG AGAGGTTCCG TTATTCTGAA 
GACGACGTGG TCAATTACCT CCTGCAGATC CTACAGGGGT TGGAGTACCT CCATGAACAA 
AAGGTCCTGC ACCTCGATAT CAAACCTGAG AACGTCATTG TGTCCTACAT GAACACTGTC 
AAGATCATCG ATTTGGGGAG TGCCCAGAAC TTTACACCGC TGGTCCTGCG ACCCCTCGGC 
AAACGCATAG GGACCCTGGA ATATATGTCC CCCGAAATGC TCAAAGGTGA CGCAGTAGGC 
CCAGCAGCTG ATATTTGGGG TGTAGGCGTT CTGACTTACA TAATGCTCAG CGGCCGCTCT 
CCGTTCTTTG AGCTGGACCC CGTGGAAACC GAGAACAAGA TCTTGTCGGG ACGATTTGAT 
ATCTTTAAAT TGTATTCCAA CGCCTCCCAA AGTGCTTCCC TCTTCATTCG CAAGATCCTG 
ACTATATATC CTTGGAGCCG CCCATCACTT AAGGAATGTT TTTCCAATCC CTGGCTCCAG 
GATGCCTACC TAATGAAGCT TAGACGCCAA ACCCTAACCT TCACCACTAA CAAGCTCAAA 
GAGTTCCTCG TGGAGCAACA GAGGAGACGC GCAGACACAG CCACTAAGCA CAAAGTCCTC 
CTTCGTTCGT ACCACGGAAG CCAGCCCTCA GCTCCTGTCA CTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031748707.1
CDS	948..11375
Translation	MPNQYQDTVTEELEEKIKKPKSSVGSQASGQDSRPVTPLSETSGRVSILRASPKLVRSGSKIFEKLRCLEERRKSLDQTDSPFPVHSWLPLRKARSFDQPGAEGLGAGLGSSNEELRDDLRDGVRSEIGGFTLRYPSLRHKTTSLDDRGALYGRISDIETRFSQELTRIKRTVSQQQLVRSSQDLSGRSPSPQRSLAAQPAPVDPKLTKVSTAPKPSETEEKQPARKPLVRSFATVNQLSEIKEPLGFSSQPPTLPSKSSNGSAVYQDAVRHSETNMKSQPHLTGKIIAPKTVSPQVFKAMTLPAQSQKEAVGDTQPKRVPDVGSVPPPPSTHAPAALEIAQRGLVKEKGTVSEEKKYLPWALPVGDNNNSQMPQGKGGGQAKKHSDAHTSSKSNKSGRSKGKSRRSRPLSPELESSDDSYVSAGEDPAEAPVFEIPLQSALVNAGTEVLLKCIISGNPSPEVFWRKDHVLLKNSPTHQIRVEGERHTLLLRWALPSDSGIYTVTARNEVGEASSCGALTVKPAPTKESPAHRGTPRDLLSPITSDEEYLSPQEDLSEPTTPQHKMAGKGPAFKAPPTFKMPLLDQKGFEGQEIVLSVQVEGEPKPIINWLKNKQQVKPGGRFRISEGAWGIFSLHIAGADKRDSGFYTCKAINEYGTKQCEAKIEVLAPPGSTSLEVLAPLQDVSVCAGEAAVFECVVSGPADMDVDWMFRGKLLQPALLDCKMRFNGKQCVLLLNSVHEDDSGVYTCKLSTARDELTCSAMLTVHPSLAPLFTRKLSDRDVIEGRTARLDCKISGTPPPVVTWTHFGQPIEESENIHVLREGGHHTLLIPHVANEDEGQYTATAHNKHGEAECSAELYVEEPRSATASHISKLEKMPSIPEEPEMQECEVEGVMMPDFLRPLQDIDVVEFREVQLECQVTGLPYPTITWYHNGQKIQSSDDRRMTQYKDIHRLVFPSVTHSHAGVYKSVISNKVGKAACYAHLYVTDLVPTPPDGPPIITSVTGRIVKLQWKPPKRLDPAIDLAQITYSVQQQAVGSPQWTVITTNLKDTCYCVRSLTKEHQYLFRVTTNTPTSHSKPSPPSESVCLFNRGPYLEEMPSILDRPEILYMVENQPLCITVTLNHVHAKVTWQRIDTVLSNVEGVYELSMPDDDQHSLTLFSPRKSDLGQLTFEAKNRYGSDSCTISIELAEAPRFESIMEDIEIGVGETARFVVVVEGKPIPDIMWYKDGVLLAESGHFSFVYDDTECSLVILNTALGDSGVYTCTARNLSGEISCKAELQVCPDEPGAGSAIQEIDILKVRRLTDFYNIHKEIGRGAFSYVRHVVEKSNGRDLAAKFIPFRGATRESARRERDILSKLRHDRIIFFYDAFEKRSALIIVMELCSQEELLERLTRKPTVSESEIRSFIRQILEGLDYLHHKNILHLDIKPENILMADTTSEQIRICDFGNAQELTPGDPQYCKYGTPEFVAPEIVNQMPISTVTDIWPVGVLAYLCLTGVSPFVGENDYTTLMNIRGYTVAFEEKMFVGLTREARGFLIKVLGNEKLRPNAEESLEHPWFKTLAKGKSISTDHLKLFQSRRKWQRSLISYKSNMVMRTIPELLQDTSNHLSIAVPKNSKEASGLSSSSDSDDLEELPFVPMPLQVQFSGSRMSLNEIPTDEEQLSQPQDNGSCREDEEEGSQAVEISSTFKEQAVQQKEATVQASPLSKHAKGTLTRNRSAEVGSSSDEETSETEKREHPRRPLKKGSSLESADVPDGEIKAARRGELRRGSSADSALLLNVSTTDGDEERDRPDKGMIKAASMELPTRCRSPGRLEDRGTLRRKFGSADEEYAQRLEMMRQRLLRGGSADGKLSGLRGPLIETLSVDKKRAEQISIRSPRSDQSSLSPIPAIKLTRAASSEAAPGREATEERVLRKTSSFSHSDTEPLVLHRRSGAPLEIPLAQLEAQRLKESPSLSALTDRLESRPQTPREMIPKSLTPEAPQLKAESGEPETQSDKEAEKGTTPLIQHHKDKDSNSLKDLVIVLPRVTPPLDKMASKTELPSIQTERAQDKIPRADVNLPATTSSNETVPTKPPPLLLPSKQSLSVPPEPLPSSVLTNEMPKSKEEYSSSRAIKAKESISKTETTDQQDKIKEPLPQAVVPLPLPMPASQVLKPSTAKVQIADSPTMPENDASLPQITGKEKLSSPAVQPVDKTAAISSPYAAMIQTLQVPEEPETVVEECSKAPLKAQPVFQEIPIATGDAKAQGISQVNNKEGKDELSQEPKLINTRSSSTENVPFISNIDSEEIFEAKFKRNRENSLTRGLKKLTRTWSEEKNLAAAHASREEEMYRPSPVGVPLEFLVPAALGLHERSRSVQDLPDAERDSSFMRKLSLRFKKTPPTERKQKPAEEVEGTGRRLSWSIGRGSSKDKKDTESLNSETGSIETITDKHLKDQKKSSQSPVLAMRKKIGSTMDRLSMKLRSQSEDRRDAERTEKKEGRTDKSSPLMSLLRRSNSEGENLKKLGIPQNQLASQVSAPSTESIESGLSIKSEMVVKDDRRSRWDRWGLSRSKKEKMTSQPSIPASLMHEDGSIVGRQYIRNESDFPPVFHIKLKDIVVLEGEPVTLSCLPAGSPSPQILWKKDKMSLERGEKVHIKSCPDGRQQLIIPAAGQREAGLYECVAANALGSVASSCTVSVARIPDPPGTPEIPQKYKNTVLVLWKTHEHNSPCTYILEKQVNGDGEWKVLSSGITDCYFNVTELQEGTVRFRVACANKAGKGPYSNVSKTIVVDAEDRKPGTPSVQKARPVGAAVSTTVPMTLRTPSTPAVAPPVVSTPKLPPSCGSALAVSSTVITTTATVSPAQPLPKGPPPATPPRKHRGILSTPPTMHKPVIATTLSADITPQTLPNNRAPSSVAPPAVCSHVSTVTVSMSPGSSVTHLRAPFSTPMQKPTPEPITKPLPVSQVQSPKAPSVPTEQTQKIALAPAVAQVKAPFVPTTAKTNEPSRLPYSVPTEPQMPSQLAAPKLSAKSPSVPIMLHIPPFKSATLPASSPTSKTISFPSPPTSPVTKPLTPTSPAYMVTSFISMPVPSPSVESSTPPTPLTPITPPTPITPPTPLTPTTPPTPISPTSPTAPFTPTSQTVPTRVIVRSVTPGKDARYTPGGRVTPSGRESTTLRQGVPQKPYTFLDEKARGRFGVIRECKENATGKHFMAKIIQYEPENKQNVLQEYEILKGLHHQRILSLHEAYITPRYLVLISENCTGRELLYCLVERFRYSEDDVVNYLLQILQGLEYLHEQKVLHLDIKPENVIVSYMNTVKIIDLGSAQNFTPLVLRPLGKRIGTLEYMSPEMLKGDAVGPAADIWGVGVLTYIMLSGRSPFFELDPVETENKILSGRFDIFKLYSNASQSASLFIRKILTIYPWSRPSLKECFSNPWLQDAYLMKLRRQTLTFTTNKLKEFLVEQQRRRADTATKHKVLLRSYHGSQPSAPVTQ

Target ORF information:

RefSeq Version	XM_031892847.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis striated muscle enriched protein kinase (speg), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031892847.1

ORF Insert Sequence:

ATGCCCAACC AGTACCAGGA CACAGTGACT GAGGAGTTAG AAGAGAAGAT TAAGAAGCCA 
AAGTCCTCTG TTGGCTCTCA GGCTAGTGGC CAAGATTCTC GCCCTGTAAC TCCACTAAGT 
GAGACATCAG GGCGGGTGTC TATCCTTAGG GCATCCCCAA AGCTAGTCCG CTCAGGCTCT 
AAGATCTTTG AAAAGCTTAG GTGTCTCGAA GAGAGGAGAA AAAGTCTGGA CCAGACAGAC 
AGCCCCTTTC CAGTGCACTC ATGGTTGCCT CTCCGTAAGG CACGATCCTT TGATCAGCCG 
GGGGCTGAGG GGCTTGGTGC TGGTCTAGGT TCCTCAAACG AAGAGCTAAG GGATGATCTT 
AGAGACGGGG TAAGATCAGA AATAGGTGGG TTTACTCTGA GGTACCCCTC TCTGAGACAC 
AAGACTACCT CACTGGATGA CAGGGGAGCT CTCTATGGAC GAATAAGTGA CATTGAGACC 
CGCTTCTCAC AAGAGCTGAC TCGCATCAAA AGAACTGTGT CCCAGCAACA GCTAGTCCGT 
TCCTCACAGG ATCTATCTGG AAGATCACCG TCACCCCAAA GATCCCTAGC TGCCCAGCCT 
GCTCCTGTGG ACCCTAAGCT AACCAAAGTG TCAACGGCTC CAAAGCCTTC TGAAACAGAA 
GAGAAGCAGC CAGCAAGGAA GCCACTAGTC AGAAGCTTTG CCACCGTCAA CCAACTGTCA 
GAAATTAAGG AACCTCTGGG GTTTTCAAGC CAACCCCCTA CACTGCCATC AAAATCATCC 
AATGGTTCTG CAGTATATCA GGATGCAGTG AGGCACTCGG AAACAAATAT GAAATCACAA 
CCACACCTTA CAGGAAAAAT CATTGCACCA AAAACTGTTT CCCCACAAGT ATTTAAAGCT 
ATGACTCTCC CCGCACAATC CCAAAAAGAG GCAGTCGGCG ACACACAGCC GAAGAGAGTT 
CCTGATGTTG GTAGCGTGCC TCCGCCACCC AGCACACATG CCCCGGCAGC CCTAGAGATA 
GCTCAGAGGG GACTTGTTAA AGAGAAGGGG ACTGTAAGTG AAGAGAAGAA GTATCTACCC 
TGGGCACTTC CTGTAGGAGA CAATAACAAC AGCCAAATGC CACAGGGTAA GGGTGGGGGA 
CAGGCCAAAA AGCACAGCGA TGCACACACC AGCAGCAAAA GCAACAAAAG CGGCAGAAGC 
AAAGGGAAGA GTCGCCGAAG CAGGCCATTG TCACCAGAAC TTGAGTCATC GGATGATTCC 
TACGTATCGG CGGGAGAGGA TCCAGCAGAG GCGCCTGTGT TTGAGATTCC TTTGCAAAGT 
GCACTAGTAA ACGCCGGCAC TGAGGTCTTA CTGAAATGTA TTATATCTGG AAACCCAAGT 
CCAGAAGTGT TTTGGAGAAA GGATCACGTG TTGCTGAAAA ACAGTCCGAC CCATCAAATA 
CGAGTAGAGG GCGAGAGACA CACGTTGCTA CTGCGCTGGG CACTGCCCTC AGACTCGGGT 
ATCTATACAG TGACTGCGCG GAATGAAGTT GGGGAAGCCA GCAGCTGCGG TGCCCTCACT 
GTAAAACCAG CACCTACCAA AGAGTCGCCG GCACATCGGG GAACACCTCG TGACCTGCTC 
AGCCCAATAA CATCGGATGA AGAGTATCTA AGCCCCCAGG AAGATTTAAG TGAGCCTACC 
ACCCCCCAAC ATAAGATGGC AGGCAAAGGC CCCGCATTCA AAGCTCCACC CACTTTCAAG 
ATGCCTCTTT TGGACCAGAA AGGGTTTGAA GGCCAAGAGA TTGTTCTCAG CGTTCAGGTG 
GAAGGGGAAC CCAAGCCTAT TATCAACTGG CTGAAGAACA AGCAGCAGGT GAAGCCTGGT 
GGAAGGTTCC GCATATCAGA AGGTGCTTGG GGGATCTTCT CGCTACATAT TGCCGGGGCT 
GATAAACGAG ATTCCGGATT CTACACCTGT AAAGCAATAA ATGAGTATGG GACAAAGCAA 
TGTGAAGCCA AGATAGAGGT TCTAGCACCC CCTGGCAGTA CCAGCTTGGA GGTTCTGGCC 
CCCTTGCAGG ATGTGAGTGT GTGCGCTGGG GAGGCAGCGG TGTTTGAATG CGTGGTGTCG 
GGCCCCGCCG ATATGGACGT GGACTGGATG TTCCGGGGCA AGCTGCTCCA GCCAGCACTG 
CTGGACTGTA AGATGCGTTT CAACGGAAAG CAGTGTGTGC TGCTGCTGAA TTCAGTCCAT 
GAAGATGACA GTGGGGTCTA CACCTGCAAA CTGAGCACTG CAAGAGATGA GCTGACCTGT 
AGCGCTATGC TAACTGTGCA CCCATCGCTG GCGCCTCTCT TCACTCGCAA GTTGTCAGAT 
CGAGATGTTA TAGAGGGACG CACAGCTCGG CTGGACTGTA AAATCAGTGG CACCCCGCCC 
CCTGTTGTCA CGTGGACTCA TTTTGGACAG CCCATAGAGG AAAGTGAGAA CATCCACGTT 
TTGAGAGAAG GTGGACATCA CACCTTACTG ATACCCCACG TGGCGAATGA GGACGAGGGT 
CAGTACACAG CGACTGCCCA TAACAAGCAT GGGGAGGCCG AATGCTCTGC TGAACTCTAC 
GTGGAGGAGC CTCGTTCTGC TACAGCATCT CATATCTCCA AGCTGGAGAA GATGCCATCT 
ATTCCAGAAG AGCCAGAGAT GCAGGAGTGT GAGGTAGAGG GTGTGATGAT GCCGGATTTC 
CTGCGACCTC TGCAGGACAT TGACGTGGTG GAATTTCGGG AGGTACAACT TGAGTGTCAG 
GTAACCGGTC TGCCGTACCC TACCATCACC TGGTATCACA ATGGTCAGAA GATCCAGAGC 
TCTGATGATC GCAGAATGAC TCAATATAAA GACATCCACC GCCTGGTTTT CCCCTCTGTT 
ACTCATTCTC ATGCCGGAGT CTATAAAAGT GTCATCTCAA ACAAAGTGGG GAAAGCAGCC 
TGCTATGCGC ACCTGTATGT CACAGATTTG GTACCCACTC CTCCAGATGG TCCCCCCATC 
ATTACCTCTG TAACTGGGAG AATTGTGAAG CTCCAGTGGA AGCCACCCAA GAGATTGGAC 
CCAGCCATTG ATCTGGCTCA GATTACATAC TCAGTCCAGC AGCAAGCGGT GGGATCTCCC 
CAATGGACAG TCATCACCAC CAACCTAAAA GACACTTGTT ATTGTGTCAG ATCTCTGACC 
AAGGAACATC AGTATCTTTT CCGTGTGACC ACAAACACCC CAACTTCTCA CAGTAAACCT 
TCCCCTCCCA GTGAAAGCGT TTGTCTCTTT AATAGAGGTC CCTACCTGGA GGAGATGCCT 
TCTATACTGG ATCGGCCTGA AATCTTGTAT ATGGTGGAAA ATCAGCCTCT TTGCATAACC 
GTGACACTGA ACCACGTACA TGCCAAAGTC ACGTGGCAGA GAATAGACAC CGTTCTGAGC 
AATGTCGAGG GTGTGTATGA GCTGAGTATG CCGGATGACG ACCAGCACTC GCTCACGTTG 
TTCAGCCCAA GGAAGTCTGA CCTGGGGCAG CTGACATTTG AGGCTAAAAA CCGCTACGGA 
TCAGATAGCT GCACTATTTC CATCGAGCTC GCAGAGGCTC CTCGCTTTGA GTCCATCATG 
GAAGACATAG AGATCGGTGT TGGTGAAACA GCTCGTTTTG TGGTCGTGGT GGAAGGGAAG 
CCTATTCCAG ACATCATGTG GTACAAAGAT GGGGTACTTT TGGCAGAGAG CGGTCACTTC 
AGCTTTGTGT ATGATGATAC CGAGTGCTCT CTGGTGATCT TAAACACGGC ACTTGGTGAC 
TCCGGTGTGT ACACCTGCAC GGCACGGAAC CTTTCAGGGG AAATTTCCTG CAAAGCGGAG 
CTGCAAGTGT GCCCAGATGA ACCAGGGGCA GGATCTGCAA TACAGGAAAT AGATATACTC 
AAAGTTCGTA GATTGACTGA TTTCTACAAC ATCCATAAAG AAATTGGCAG AGGGGCCTTC 
TCCTATGTCC GACATGTGGT AGAAAAGAGC AACGGGCGAG ATCTTGCTGC TAAATTCATA 
CCGTTCCGTG GGGCAACCAG GGAGAGTGCA CGGAGGGAGC GGGACATACT GTCCAAGCTC 
AGACACGACA GGATCATCTT CTTCTACGAT GCATTTGAGA AAAGAAGTGC TCTAATCATT 
GTCATGGAAC TCTGTTCTCA AGAGGAGCTC CTAGAGAGGT TGACAAGAAA GCCTACAGTG 
AGCGAATCAG AGATCCGCTC GTTTATACGG CAAATCCTAG AAGGGTTAGA TTATTTGCAC 
CATAAAAACA TTCTGCATTT GGACATAAAG CCAGAGAACA TCCTGATGGC CGATACGACT 
AGTGAACAGA TTCGCATTTG TGACTTTGGA AACGCTCAGG AACTAACCCC GGGGGATCCT 
CAGTACTGCA AATACGGAAC CCCGGAATTT GTGGCGCCCG AAATTGTGAA CCAGATGCCA 
ATTTCTACAG TAACGGACAT ATGGCCAGTT GGAGTCCTGG CATATTTATG CCTTACCGGA 
GTGTCGCCCT TTGTTGGGGA GAATGATTAC ACCACTCTGA TGAATATCCG GGGTTACACT 
GTGGCATTTG AAGAGAAGAT GTTTGTTGGT CTCACACGAG AAGCACGTGG TTTCCTAATC 
AAAGTCTTGG GCAATGAGAA GCTGAGGCCT AATGCTGAAG AGAGTTTGGA GCACCCCTGG 
TTCAAAACTT TAGCGAAAGG GAAAAGCATC AGCACAGACC ATCTGAAGCT GTTCCAGTCT 
CGCCGTAAAT GGCAGCGCTC ACTTATCAGC TATAAGTCCA ATATGGTGAT GAGAACCATT 
CCAGAGCTGC TTCAGGACAC AAGCAATCAC TTGTCCATTG CTGTACCCAA GAATTCCAAG 
GAAGCATCCG GATTGTCATC CTCCTCTGAT TCTGATGACC TGGAAGAGCT TCCCTTTGTC 
CCTATGCCTC TCCAGGTCCA ATTCTCTGGA TCACGCATGT CCTTGAATGA GATCCCAACA 
GATGAGGAAC AACTGTCTCA GCCCCAGGAC AATGGCAGCT GTAGAGAAGA TGAGGAAGAA 
GGAAGTCAGG CAGTGGAAAT TAGTAGCACA TTTAAAGAAC AAGCAGTTCA GCAGAAAGAA 
GCAACAGTCC AAGCAAGTCC ATTGTCCAAG CATGCCAAAG GAACCCTTAC AAGGAACAGG 
TCTGCTGAAG TTGGTTCTTC TTCTGATGAG GAAACTTCAG AAACTGAAAA AAGAGAGCAT 
CCTCGTAGGC CTCTGAAAAA AGGTTCCAGC TTAGAATCTG CCGACGTTCC TGATGGGGAA 
ATTAAAGCAG CTCGTAGAGG AGAGCTGAGG AGGGGAAGCT CTGCAGATAG TGCTCTTTTG 
CTCAATGTGT CTACAACAGA TGGAGATGAA GAGAGAGATC GACCAGACAA GGGAATGATA 
AAAGCTGCCT CAATGGAGCT ACCAACCAGA TGCAGGAGCC CAGGTAGATT GGAGGATCGA 
GGCACACTAC GAAGAAAGTT TGGATCAGCT GATGAAGAAT ATGCTCAGCG GTTAGAAATG 
ATGAGACAGA GGCTATTGAG GGGAGGTTCG GCAGATGGAA AACTCAGTGG TCTTAGAGGC 
CCACTCATAG AAACACTTTC TGTAGACAAG AAGCGAGCAG AGCAGATATC TATACGCTCC 
CCAAGAAGTG ATCAGTCTTC TCTGAGTCCT ATTCCCGCCA TCAAACTAAC CCGAGCTGCT 
TCCAGTGAGG CTGCGCCTGG TAGAGAAGCA ACTGAAGAAA GAGTCTTAAG GAAAACCAGT 
TCCTTTTCTC ACAGCGACAC AGAGCCACTG GTCTTGCATC GCAGGTCAGG GGCACCCCTA 
GAAATACCTC TTGCTCAGTT AGAGGCACAG CGTCTTAAAG AGTCTCCCTC TCTTTCAGCA 
TTGACAGACC GATTAGAATC ACGTCCTCAA ACACCGCGAG AGATGATTCC CAAATCTCTA 
ACTCCAGAAG CACCGCAATT GAAGGCAGAA AGTGGGGAGC CAGAAACTCA GAGTGATAAG 
GAAGCTGAGA AGGGCACCAC ACCTCTTATT CAGCATCATA AAGACAAAGA CTCAAATTCA 
TTGAAGGACT TGGTTATTGT TCTTCCAAGA GTGACTCCAC CTTTGGATAA AATGGCATCT 
AAAACAGAGT TACCAAGTAT TCAAACAGAG AGGGCACAGG ACAAAATCCC TAGAGCAGAT 
GTGAACCTGC CAGCTACTAC TTCATCAAAT GAAACTGTGC CAACAAAGCC ACCACCCTTA 
CTTTTGCCTT CTAAGCAGTC TCTTTCTGTG CCACCTGAAC CTTTACCCTC TTCAGTACTG 
ACAAATGAGA TGCCTAAATC TAAAGAGGAG TACTCGTCAT CAAGGGCCAT TAAGGCCAAG 
GAATCCATAT CAAAAACAGA AACAACAGAC CAGCAAGATA AAATTAAAGA ACCTTTACCA 
CAAGCTGTTG TTCCATTACC ATTGCCTATG CCAGCCTCCC AAGTGCTAAA ACCTTCCACT 
GCAAAAGTAC AGATAGCAGA TTCTCCAACA ATGCCTGAGA ATGATGCATC ACTTCCACAG 
ATAACAGGTA AAGAGAAACT ATCTTCTCCA GCTGTCCAAC CAGTAGATAA AACAGCAGCT 
ATATCATCAC CTTATGCTGC TATGATTCAA ACACTTCAAG TGCCCGAGGA ACCTGAGACA 
GTTGTTGAGG AATGTTCAAA GGCCCCATTA AAAGCTCAGC CAGTATTTCA AGAAATTCCT 
ATAGCCACTG GAGATGCAAA GGCTCAAGGA ATATCACAAG TAAACAATAA AGAAGGCAAG 
GATGAGCTAT CACAAGAACC CAAGCTGATT AACACCAGAT CTTCCAGCAC AGAGAATGTC 
CCATTTATCA GCAACATTGA TTCAGAAGAA ATCTTTGAGG CTAAGTTCAA GAGGAACCGT 
GAAAATTCTC TTACCCGAGG TCTAAAAAAG CTTACCAGGA CTTGGTCAGA GGAGAAGAAT 
TTAGCTGCAG CCCATGCATC TCGGGAAGAA GAAATGTACA GGCCTAGTCC TGTGGGTGTC 
CCACTAGAGT TCCTTGTTCC TGCAGCTCTT GGGCTACATG AAAGATCAAG ATCAGTGCAA 
GATCTGCCAG ATGCTGAGAG GGATTCAAGC TTTATGAGAA AGCTTTCTTT GAGATTTAAG 
AAAACTCCAC CAACTGAAAG GAAGCAGAAA CCAGCAGAAG AAGTAGAAGG TACTGGTAGA 
CGACTTTCAT GGTCAATAGG TAGAGGAAGT TCCAAAGATA AGAAGGATAC AGAGAGCCTG 
AACTCTGAAA CAGGCTCAAT AGAAACCATC ACAGACAAAC ATCTAAAGGA CCAGAAAAAG 
AGTAGTCAAT CACCAGTGTT GGCCATGAGA AAGAAAATAG GCAGTACTAT GGATCGACTT 
TCTATGAAAC TAAGGAGCCA ATCAGAAGAT AGAAGGGATG CAGAGAGAAC AGAGAAGAAA 
GAAGGGCGTA CAGACAAAAG TTCTCCATTA ATGTCTTTAC TTAGGAGATC CAACTCAGAA 
GGAGAGAACT TAAAGAAACT AGGTATTCCC CAGAACCAAC TTGCTTCCCA GGTTTCAGCT 
CCATCAACTG AGTCTATTGA ATCTGGTCTC AGCATCAAAT CTGAAATGGT AGTCAAGGAT 
GATAGGCGCT CAAGGTGGGA TCGTTGGGGC CTGTCACGTA GCAAAAAGGA GAAGATGACA 
TCGCAGCCAA GTATTCCAGC CAGTCTGATG CATGAAGATG GCAGTATTGT TGGCAGACAA 
TACATTCGTA ATGAGTCAGA CTTCCCCCCA GTCTTCCATA TCAAGCTGAA GGACATCGTG 
GTGCTAGAGG GAGAGCCGGT GACTCTGTCC TGCTTACCTG CTGGCAGCCC TTCCCCCCAG 
ATCCTGTGGA AGAAAGATAA GATGTCCTTG GAAAGAGGAG AAAAGGTGCA TATAAAATCC 
TGCCCCGATG GCAGACAGCA GCTCATTATT CCCGCCGCCG GGCAGAGGGA AGCCGGTCTG 
TATGAATGCG TAGCAGCCAA TGCCCTGGGC TCAGTGGCCA GCTCCTGCAC AGTGTCTGTG 
GCAAGAATAC CTGATCCCCC GGGGACCCCA GAGATCCCAC AGAAGTATAA GAACACGGTG 
CTGGTTCTAT GGAAGACCCA CGAGCACAAC TCCCCTTGCA CCTACATCCT GGAGAAACAG 
GTTAATGGTG ATGGGGAATG GAAGGTTCTA AGTTCTGGAA TCACCGACTG TTACTTCAAT 
GTGACGGAGC TCCAGGAAGG CACTGTCAGG TTCCGCGTCG CTTGTGCCAA CAAAGCAGGA 
AAGGGGCCCT ACAGCAATGT GTCCAAGACA ATTGTCGTAG ATGCAGAAGA TCGTAAGCCA 
GGAACACCCT CTGTCCAGAA GGCACGGCCC GTGGGAGCTG CAGTGTCCAC TACTGTTCCA 
ATGACTCTCC GGACCCCTTC TACCCCAGCT GTTGCGCCCC CTGTTGTCTC TACCCCCAAA 
TTACCTCCAT CTTGTGGATC TGCTCTTGCA GTTTCCTCAA CAGTTATCAC TACAACCGCA 
ACTGTGTCTC CAGCCCAGCC TCTCCCCAAA GGGCCTCCAC CTGCAACTCC TCCACGGAAA 
CACCGAGGGA TACTCTCCAC TCCCCCAACC ATGCACAAGC CTGTCATTGC CACCACTCTC 
AGTGCCGATA TTACTCCCCA AACTCTGCCC AATAACAGGG CTCCATCTTC TGTGGCTCCT 
CCTGCCGTAT GTTCTCATGT CTCCACGGTG ACTGTGTCTA TGTCACCAGG TTCTTCGGTG 
ACCCATTTAA GGGCCCCCTT CTCTACCCCC ATGCAGAAAC CTACCCCAGA ACCAATAACA 
AAACCATTAC CAGTGTCACA AGTACAATCA CCCAAAGCTC CTTCTGTGCC AACAGAGCAA 
ACCCAGAAAA TTGCCCTTGC CCCGGCTGTT GCCCAGGTTA AAGCACCTTT TGTGCCAACT 
ACTGCAAAAA CTAACGAACC ATCACGTCTT CCATATAGTG TCCCTACGGA ACCACAGATG 
CCATCACAGT TAGCAGCTCC AAAACTATCT GCCAAGTCCC CCTCTGTTCC AATTATGCTG 
CACATTCCCC CCTTTAAATC AGCAACCCTA CCTGCTTCCA GTCCCACTTC CAAAACCATA 
TCTTTTCCCT CTCCTCCTAC AAGCCCAGTA ACCAAACCCT TAACACCTAC CTCCCCAGCT 
TATATGGTCA CTTCCTTTAT ATCAATGCCT GTGCCTTCAC CCTCTGTTGA GTCCTCCACT 
CCACCTACTC CACTTACCCC CATCACCCCA CCTACCCCCA TCACCCCACC TACCCCACTT 
ACCCCCACCA CCCCTCCTAC CCCCATTAGC CCCACTTCCC CAACCGCCCC ATTTACCCCC 
ACTTCCCAAA CTGTCCCTAC ACGGGTGATA GTAAGAAGTG TCACCCCCGG GAAGGATGCA 
CGTTATACAC CCGGAGGAAG AGTCACACCC TCGGGGAGAG AAAGCACCAC CCTCCGACAA 
GGGGTACCCC AAAAACCTTA CACTTTCTTG GATGAGAAAG CAAGGGGGCG GTTTGGAGTG 
ATCCGAGAGT GTAAGGAGAA TGCAACTGGG AAGCACTTCA TGGCAAAGAT AATCCAGTAC 
GAACCAGAAA ACAAGCAAAA CGTGCTGCAG GAGTATGAGA TCCTCAAGGG TCTCCACCAT 
CAACGAATCT TGAGTCTTCA TGAAGCTTAT ATTACCCCTC GCTACCTTGT GTTGATTTCT 
GAAAACTGCA CAGGACGGGA ACTGCTTTAC TGCCTAGTTG AGAGGTTCCG TTATTCTGAA 
GACGACGTGG TCAATTACCT CCTGCAGATC CTACAGGGGT TGGAGTACCT CCATGAACAA 
AAGGTCCTGC ACCTCGATAT CAAACCTGAG AACGTCATTG TGTCCTACAT GAACACTGTC 
AAGATCATCG ATTTGGGGAG TGCCCAGAAC TTTACACCGC TGGTCCTGCG ACCCCTCGGC 
AAACGCATAG GGACCCTGGA ATATATGTCC CCCGAAATGC TCAAAGGTGA CGCAGTAGGC 
CCAGCAGCTG ATATTTGGGG TGTAGGCGTT CTGACTTACA TAATGCTCAG CGGCCGCTCT 
CCGTTCTTTG AGCTGGACCC CGTGGAAACC GAGAACAAGA TCTTGTCGGG ACGATTTGAT 
ATCTTTAAAT TGTATTCCAA CGCCTCCCAA AGTGCTTCCC TCTTCATTCG CAAGATCCTG 
ACTATATATC CTTGGAGCCG CCCATCACTT AAGGAATGTT TTTCCAATCC CTGGCTCCAG 
GATGCCTACC TAATGAAGCT TAGACGCCAA ACCCTAACCT TCACCACTAA CAAGCTCAAA 
GAGTTCCTCG TGGAGCAACA GAGGAGACGC GCAGACACAG CCACTAAGCA CAAAGTCCTC 
CTTCGTTCGT ACCACGGAAG CCAGCCCTCA GCTCCTGTCA CTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

speg ( XM_031892848.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031748708.1 Xenopus tropicalis striated muscle enriched protein kinase (speg), transcript variant X5, mRNA.

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CloneID	OXa66226
Clone ID Related Accession (Same CDS sequence)	XM_031892848.1
Accession Version	XM_031892848.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	striated muscle preferentially expressed protein kinase isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGCA AAGGCCCCGC ATTCAAAGCT CCACCCACTT TCAAGATGCC TCTTTTGGAC 
CAGAAAGGGT TTGAAGGCCA AGAGATTGTT CTCAGCGTTC AGGTGGAAGG GGAACCCAAG 
CCTATTATCA ACTGGCTGAA GAACAAGCAG CAGGTGAAGC CTGGTGGAAG GTTCCGCATA 
TCAGAAGGTG CTTGGGGGAT CTTCTCGCTA CATATTGCCG GGGCTGATAA ACGAGATTCC 
GGATTCTACA CCTGTAAAGC AATAAATGAG TATGGGACAA AGCAATGTGA AGCCAAGATA 
GAGGTTCTAG CACCCCCTGG CAGTACCAGC TTGGAGGTTC TGGCCCCCTT GCAGGATGTG 
AGTGTGTGCG CTGGGGAGGC AGCGGTGTTT GAATGCGTGG TGTCGGGCCC CGCCGATATG 
GACGTGGACT GGATGTTCCG GGGCAAGCTG CTCCAGCCAG CACTGCTGGA CTGTAAGATG 
CGTTTCAACG GAAAGCAGTG TGTGCTGCTG CTGAATTCAG TCCATGAAGA TGACAGTGGG 
GTCTACACCT GCAAACTGAG CACTGCAAGA GATGAGCTGA CCTGTAGCGC TATGCTAACT 
GTGCACCCAT CGCTGGCGCC TCTCTTCACT CGCAAGTTGT CAGATCGAGA TGTTATAGAG 
GGACGCACAG CTCGGCTGGA CTGTAAAATC AGTGGCACCC CGCCCCCTGT TGTCACGTGG 
ACTCATTTTG GACAGCCCAT AGAGGAAAGT GAGAACATCC ACGTTTTGAG AGAAGGTGGA 
CATCACACCT TACTGATACC CCACGTGGCG AATGAGGACG AGGGTCAGTA CACAGCGACT 
GCCCATAACA AGCATGGGGA GGCCGAATGC TCTGCTGAAC TCTACGTGGA GGAGCCTCGT 
TCTGCTACAG CATCTCATAT CTCCAAGCTG GAGAAGATGC CATCTATTCC AGAAGAGCCA 
GAGATGCAGG AGTGTGAGGT AGAGGGTGTG ATGATGCCGG ATTTCCTGCG ACCTCTGCAG 
GACATTGACG TGGTGGAATT TCGGGAGGTA CAACTTGAGT GTCAGGTAAC CGGTCTGCCG 
TACCCTACCA TCACCTGGTA TCACAATGGT CAGAAGATCC AGAGCTCTGA TGATCGCAGA 
ATGACTCAAT ATAAAGACAT CCACCGCCTG GTTTTCCCCT CTGTTACTCA TTCTCATGCC 
GGAGTCTATA AAAGTGTCAT CTCAAACAAA GTGGGGAAAG CAGCCTGCTA TGCGCACCTG 
TATGTCACAG ATTTGGTACC CACTCCTCCA GATGGTCCCC CCATCATTAC CTCTGTAACT 
GGGAGAATTG TGAAGCTCCA GTGGAAGCCA CCCAAGAGAT TGGACCCAGC CATTGATCTG 
GCTCAGATTA CATACTCAGT CCAGCAGCAA GCGGTGGGAT CTCCCCAATG GACAGTCATC 
ACCACCAACC TAAAAGACAC TTGTTATTGT GTCAGATCTC TGACCAAGGA ACATCAGTAT 
CTTTTCCGTG TGACCACAAA CACCCCAACT TCTCACAGTA AACCTTCCCC TCCCAGTGAA 
AGCGTTTGTC TCTTTAATAG AGGTCCCTAC CTGGAGGAGA TGCCTTCTAT ACTGGATCGG 
CCTGAAATCT TGTATATGGT GGAAAATCAG CCTCTTTGCA TAACCGTGAC ACTGAACCAC 
GTACATGCCA AAGTCACGTG GCAGAGAATA GACACCGTTC TGAGCAATGT CGAGGGTGTG 
TATGAGCTGA GTATGCCGGA TGACGACCAG CACTCGCTCA CGTTGTTCAG CCCAAGGAAG 
TCTGACCTGG GGCAGCTGAC ATTTGAGGCT AAAAACCGCT ACGGATCAGA TAGCTGCACT 
ATTTCCATCG AGCTCGCAGA GGCTCCTCGC TTTGAGTCCA TCATGGAAGA CATAGAGATC 
GGTGTTGGTG AAACAGCTCG TTTTGTGGTC GTGGTGGAAG GGAAGCCTAT TCCAGACATC 
ATGTGGTACA AAGATGGGGT ACTTTTGGCA GAGAGCGGTC ACTTCAGCTT TGTGTATGAT 
GATACCGAGT GCTCTCTGGT GATCTTAAAC ACGGCACTTG GTGACTCCGG TGTGTACACC 
TGCACGGCAC GGAACCTTTC AGGGGAAATT TCCTGCAAAG CGGAGCTGCA AGTGTGCCCA 
GATGAACCAG GGGCAGGATC TGCAATACAG GAAATAGATA TACTCAAAGT TCGTAGATTG 
ACTGATTTCT ACAACATCCA TAAAGAAATT GGCAGAGGGG CCTTCTCCTA TGTCCGACAT 
GTGGTAGAAA AGAGCAACGG GCGAGATCTT GCTGCTAAAT TCATACCGTT CCGTGGGGCA 
ACCAGGGAGA GTGCACGGAG GGAGCGGGAC ATACTGTCCA AGCTCAGACA CGACAGGATC 
ATCTTCTTCT ACGATGCATT TGAGAAAAGA AGTGCTCTAA TCATTGTCAT GGAACTCTGT 
TCTCAAGAGG AGCTCCTAGA GAGGTTGACA AGAAAGCCTA CAGTGAGCGA ATCAGAGATC 
CGCTCGTTTA TACGGCAAAT CCTAGAAGGG TTAGATTATT TGCACCATAA AAACATTCTG 
CATTTGGACA TAAAGCCAGA GAACATCCTG ATGGCCGATA CGACTAGTGA ACAGATTCGC 
ATTTGTGACT TTGGAAACGC TCAGGAACTA ACCCCGGGGG ATCCTCAGTA CTGCAAATAC 
GGAACCCCGG AATTTGTGGC GCCCGAAATT GTGAACCAGA TGCCAATTTC TACAGTAACG 
GACATATGGC CAGTTGGAGT CCTGGCATAT TTATGCCTTA CCGGAGTGTC GCCCTTTGTT 
GGGGAGAATG ATTACACCAC TCTGATGAAT ATCCGGGGTT ACACTGTGGC ATTTGAAGAG 
AAGATGTTTG TTGGTCTCAC ACGAGAAGCA CGTGGTTTCC TAATCAAAGT CTTGGGCAAT 
GAGAAGCTGA GGCCTAATGC TGAAGAGAGT TTGGAGCACC CCTGGTTCAA AACTTTAGCG 
AAAGGGAAAA GCATCAGCAC AGACCATCTG AAGCTGTTCC AGTCTCGCCG TAAATGGCAG 
CGCTCACTTA TCAGCTATAA GTCCAATATG GTGATGAGAA CCATTCCAGA GCTGCTTCAG 
GACACAAGCA ATCACTTGTC CATTGCTGTA CCCAAGAATT CCAAGGAAGC ATCCGGATTG 
TCATCCTCCT CTGATTCTGA TGACCTGGAA GAGCTTCCCT TTGTCCCTAT GCCTCTCCAG 
GTCCAATTCT CTGGATCACG CATGTCCTTG AATGAGATCC CAACAGATGA GGAACAACTG 
TCTCAGCCCC AGGACAATGG CAGCTGTAGA GAAGATGAGG AAGAAGGAAG TCAGGCAGTG 
GAAATTAGTA GCACATTTAA AGAACAAGCA GTTCAGCAGA AAGAAGCAAC AGTCCAAGCA 
AGTCCATTGT CCAAGCATGC CAAAGGAACC CTTACAAGGA ACAGGTCTGC TGAAGTTGGT 
TCTTCTTCTG ATGAGGAAAC TTCAGAAACT GAAAAAAGAG AGCATCCTCG TAGGCCTCTG 
AAAAAAGGTT CCAGCTTAGA ATCTGCCGAC GTTCCTGATG GGGAAATTAA AGCAGCTCGT 
AGAGGAGAGC TGAGGAGGGG AAGCTCTGCA GATAGTGCTC TTTTGCTCAA TGTGTCTACA 
ACAGATGGAG ATGAAGAGAG AGATCGACCA GACAAGGGAA TGATAAAAGC TGCCTCAATG 
GAGCTACCAA CCAGATGCAG GAGCCCAGGT AGATTGGAGG ATCGAGGCAC ACTACGAAGA 
AAGTTTGGAT CAGCTGATGA AGAATATGCT CAGCGGTTAG AAATGATGAG ACAGAGGCTA 
TTGAGGGGAG GTTCGGCAGA TGGAAAACTC AGTGGTCTTA GAGGCCCACT CATAGAAACA 
CTTTCTGTAG ACAAGAAGCG AGCAGAGCAG ATATCTATAC GCTCCCCAAG AAGTGATCAG 
TCTTCTCTGA GTCCTATTCC CGCCATCAAA CTAACCCGAG CTGCTTCCAG TGAGGCTGCG 
CCTGGTAGAG AAGCAACTGA AGAAAGAGTC TTAAGGAAAA CCAGTTCCTT TTCTCACAGC 
GACACAGAGC CACTGGTCTT GCATCGCAGG TCAGGGGCAC CCCTAGAAAT ACCTCTTGCT 
CAGTTAGAGG CACAGCGTCT TAAAGAGTCT CCCTCTCTTT CAGCATTGAC AGACCGATTA 
GAATCACGTC CTCAAACACC GCGAGAGATG ATTCCCAAAT CTCTAACTCC AGAAGCACCG 
CAATTGAAGG CAGAAAGTGG GGAGCCAGAA ACTCAGAGTG ATAAGGAAGC TGAGAAGGGC 
ACCACACCTC TTATTCAGCA TCATAAAGAC AAAGACTCAA ATTCATTGAA GGACTTGGTT 
ATTGTTCTTC CAAGAGTGAC TCCACCTTTG GATAAAATGG CATCTAAAAC AGAGTTACCA 
AGTATTCAAA CAGAGAGGGC ACAGGACAAA ATCCCTAGAG CAGATGTGAA CCTGCCAGCT 
ACTACTTCAT CAAATGAAAC TGTGCCAACA AAGCCACCAC CCTTACTTTT GCCTTCTAAG 
CAGTCTCTTT CTGTGCCACC TGAACCTTTA CCCTCTTCAG TACTGACAAA TGAGATGCCT 
AAATCTAAAG AGGAGTACTC GTCATCAAGG GCCATTAAGG CCAAGGAATC CATATCAAAA 
ACAGAAACAA CAGACCAGCA AGATAAAATT AAAGAACCTT TACCACAAGC TGTTGTTCCA 
TTACCATTGC CTATGCCAGC CTCCCAAGTG CTAAAACCTT CCACTGCAAA AGTACAGATA 
GCAGATTCTC CAACAATGCC TGAGAATGAT GCATCACTTC CACAGATAAC AGGTAAAGAG 
AAACTATCTT CTCCAGCTGT CCAACCAGTA GATAAAACAG CAGCTATATC ATCACCTTAT 
GCTGCTATGA TTCAAACACT TCAAGTGCCC GAGGAACCTG AGACAGTTGT TGAGGAATGT 
TCAAAGGCCC CATTAAAAGC TCAGCCAGTA TTTCAAGAAA TTCCTATAGC CACTGGAGAT 
GCAAAGGCTC AAGGAATATC ACAAGTAAAC AATAAAGAAG GCAAGGATGA GCTATCACAA 
GAACCCAAGC TGATTAACAC CAGATCTTCC AGCACAGAGA ATGTCCCATT TATCAGCAAC 
ATTGATTCAG AAGAAATCTT TGAGGCTAAG TTCAAGAGGA ACCGTGAAAA TTCTCTTACC 
CGAGGTCTAA AAAAGCTTAC CAGGACTTGG TCAGAGGAGA AGAATTTAGC TGCAGCCCAT 
GCATCTCGGG AAGAAGAAAT GTACAGGCCT AGTCCTGTGG GTGTCCCACT AGAGTTCCTT 
GTTCCTGCAG CTCTTGGGCT ACATGAAAGA TCAAGATCAG TGCAAGATCT GCCAGATGCT 
GAGAGGGATT CAAGCTTTAT GAGAAAGCTT TCTTTGAGAT TTAAGAAAAC TCCACCAACT 
GAAAGGAAGC AGAAACCAGC AGAAGAAGTA GAAGGTACTG GTAGACGACT TTCATGGTCA 
ATAGGTAGAG GAAGTTCCAA AGATAAGAAG GATACAGAGA GCCTGAACTC TGAAACAGGC 
TCAATAGAAA CCATCACAGA CAAACATCTA AAGGACCAGA AAAAGAGTAG TCAATCACCA 
GTGTTGGCCA TGAGAAAGAA AATAGGCAGT ACTATGGATC GACTTTCTAT GAAACTAAGG 
AGCCAATCAG AAGATAGAAG GGATGCAGAG AGAACAGAGA AGAAAGAAGG GCGTACAGAC 
AAAAGTTCTC CATTAATGTC TTTACTTAGG AGATCCAACT CAGAAGGAGA GAACTTAAAG 
AAACTAGGTA TTCCCCAGAA CCAACTTGCT TCCCAGGTTT CAGCTCCATC AACTGAGTCT 
ATTGAATCTG GTCTCAGCAT CAAATCTGAA ATGGTAGTCA AGGATGATAG GCGCTCAAGG 
TGGGATCGTT GGGGCCTGTC ACGTAGCAAA AAGGAGAAGA TGACATCGCA GCCAAGTATT 
CCAGCCAGTC TGATGCATGA AGATGGCAGT ATTGTTGGCA GACAATACAT TCGTAATGAG 
TCAGACTTCC CCCCAGTCTT CCATATCAAG CTGAAGGACA TCGTGGTGCT AGAGGGAGAG 
CCGGTGACTC TGTCCTGCTT ACCTGCTGGC AGCCCTTCCC CCCAGATCCT GTGGAAGAAA 
GATAAGATGT CCTTGGAAAG AGGAGAAAAG GTGCATATAA AATCCTGCCC CGATGGCAGA 
CAGCAGCTCA TTATTCCCGC CGCCGGGCAG AGGGAAGCCG GTCTGTATGA ATGCGTAGCA 
GCCAATGCCC TGGGCTCAGT GGCCAGCTCC TGCACAGTGT CTGTGGCAAG AATACCTGAT 
CCCCCGGGGA CCCCAGAGAT CCCACAGAAG TATAAGAACA CGGTGCTGGT TCTATGGAAG 
ACCCACGAGC ACAACTCCCC TTGCACCTAC ATCCTGGAGA AACAGGTTAA TGGTGATGGG 
GAATGGAAGG TTCTAAGTTC TGGAATCACC GACTGTTACT TCAATGTGAC GGAGCTCCAG 
GAAGGCACTG TCAGGTTCCG CGTCGCTTGT GCCAACAAAG CAGGAAAGGG GCCCTACAGC 
AATGTGTCCA AGACAATTGT CGTAGATGCA GAAGATCGTA AGCCAGGAAC ACCCTCTGTC 
CAGAAGGCAC GGCCCGTGGG AGCTGCAGTG TCCACTACTG TTCCAATGAC TCTCCGGACC 
CCTTCTACCC CAGCTGTTGC GCCCCCTGTT GTCTCTACCC CCAAATTACC TCCATCTTGT 
GGATCTGCTC TTGCAGTTTC CTCAACAGTT ATCACTACAA CCGCAACTGT GTCTCCAGCC 
CAGCCTCTCC CCAAAGGGCC TCCACCTGCA ACTCCTCCAC GGAAACACCG AGGGATACTC 
TCCACTCCCC CAACCATGCA CAAGCCTGTC ATTGCCACCA CTCTCAGTGC CGATATTACT 
CCCCAAACTC TGCCCAATAA CAGGGCTCCA TCTTCTGTGG CTCCTCCTGC CGTATGTTCT 
CATGTCTCCA CGGTGACTGT GTCTATGTCA CCAGGTTCTT CGGTGACCCA TTTAAGGGCC 
CCCTTCTCTA CCCCCATGCA GAAACCTACC CCAGAACCAA TAACAAAACC ATTACCAGTG 
TCACAAGTAC AATCACCCAA AGCTCCTTCT GTGCCAACAG AGCAAACCCA GAAAATTGCC 
CTTGCCCCGG CTGTTGCCCA GGTTAAAGCA CCTTTTGTGC CAACTACTGC AAAAACTAAC 
GAACCATCAC GTCTTCCATA TAGTGTCCCT ACGGAACCAC AGATGCCATC ACAGTTAGCA 
GCTCCAAAAC TATCTGCCAA GTCCCCCTCT GTTCCAATTA TGCTGCACAT TCCCCCCTTT 
AAATCAGCAA CCCTACCTGC TTCCAGTCCC ACTTCCAAAA CCATATCTTT TCCCTCTCCT 
CCTACAAGCC CAGTAACCAA ACCCTTAACA CCTACCTCCC CAGCTTATAT GGTCACTTCC 
TTTATATCAA TGCCTGTGCC TTCACCCTCT GTTGAGTCCT CCACTCCACC TACTCCACTT 
ACCCCCATCA CCCCACCTAC CCCCATCACC CCACCTACCC CACTTACCCC CACCACCCCT 
CCTACCCCCA TTAGCCCCAC TTCCCCAACC GCCCCATTTA CCCCCACTTC CCAAACTGTC 
CCTACACGGG TGATAGTAAG AAGTGTCACC CCCGGGAAGG ATGCACGTTA TACACCCGGA 
GGAAGAGTCA CACCCTCGGG GAGAGAAAGC ACCACCCTCC GACAAGGGGT ACCCCAAAAA 
CCTTACACTT TCTTGGATGA GAAAGCAAGG GGGCGGTTTG GAGTGATCCG AGAGTGTAAG 
GAGAATGCAA CTGGGAAGCA CTTCATGGCA AAGATAATCC AGTACGAACC AGAAAACAAG 
CAAAACGTGC TGCAGGAGTA TGAGATCCTC AAGGGTCTCC ACCATCAACG AATCTTGAGT 
CTTCATGAAG CTTATATTAC CCCTCGCTAC CTTGTGTTGA TTTCTGAAAA CTGCACAGGA 
CGGGAACTGC TTTACTGCCT AGTTGAGAGG TTCCGTTATT CTGAAGACGA CGTGGTCAAT 
TACCTCCTGC AGATCCTACA GGGGTTGGAG TACCTCCATG AACAAAAGGT CCTGCACCTC 
GATATCAAAC CTGAGAACGT CATTGTGTCC TACATGAACA CTGTCAAGAT CATCGATTTG 
GGGAGTGCCC AGAACTTTAC ACCGCTGGTC CTGCGACCCC TCGGCAAACG CATAGGGACC 
CTGGAATATA TGTCCCCCGA AATGCTCAAA GGTGACGCAG TAGGCCCAGC AGCTGATATT 
TGGGGTGTAG GCGTTCTGAC TTACATAATG CTCAGCGGCC GCTCTCCGTT CTTTGAGCTG 
GACCCCGTGG AAACCGAGAA CAAGATCTTG TCGGGACGAT TTGATATCTT TAAATTGTAT 
TCCAACGCCT CCCAAAGTGC TTCCCTCTTC ATTCGCAAGA TCCTGACTAT ATATCCTTGG 
AGCCGCCCAT CACTTAAGGA ATGTTTTTCC AATCCCTGGC TCCAGGATGC CTACCTAATG 
AAGCTTAGAC GCCAAACCCT AACCTTCACC ACTAACAAGC TCAAAGAGTT CCTCGTGGAG 
CAACAGAGGA GACGCGCAGA CACAGCCACT AAGCACAAAG TCCTCCTTCG TTCGTACCAC 
GGAAGCCAGC CCTCAGCTCC TGTCACTCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031748708.1
CDS	374..9106
Translation	MAGKGPAFKAPPTFKMPLLDQKGFEGQEIVLSVQVEGEPKPIINWLKNKQQVKPGGRFRISEGAWGIFSLHIAGADKRDSGFYTCKAINEYGTKQCEAKIEVLAPPGSTSLEVLAPLQDVSVCAGEAAVFECVVSGPADMDVDWMFRGKLLQPALLDCKMRFNGKQCVLLLNSVHEDDSGVYTCKLSTARDELTCSAMLTVHPSLAPLFTRKLSDRDVIEGRTARLDCKISGTPPPVVTWTHFGQPIEESENIHVLREGGHHTLLIPHVANEDEGQYTATAHNKHGEAECSAELYVEEPRSATASHISKLEKMPSIPEEPEMQECEVEGVMMPDFLRPLQDIDVVEFREVQLECQVTGLPYPTITWYHNGQKIQSSDDRRMTQYKDIHRLVFPSVTHSHAGVYKSVISNKVGKAACYAHLYVTDLVPTPPDGPPIITSVTGRIVKLQWKPPKRLDPAIDLAQITYSVQQQAVGSPQWTVITTNLKDTCYCVRSLTKEHQYLFRVTTNTPTSHSKPSPPSESVCLFNRGPYLEEMPSILDRPEILYMVENQPLCITVTLNHVHAKVTWQRIDTVLSNVEGVYELSMPDDDQHSLTLFSPRKSDLGQLTFEAKNRYGSDSCTISIELAEAPRFESIMEDIEIGVGETARFVVVVEGKPIPDIMWYKDGVLLAESGHFSFVYDDTECSLVILNTALGDSGVYTCTARNLSGEISCKAELQVCPDEPGAGSAIQEIDILKVRRLTDFYNIHKEIGRGAFSYVRHVVEKSNGRDLAAKFIPFRGATRESARRERDILSKLRHDRIIFFYDAFEKRSALIIVMELCSQEELLERLTRKPTVSESEIRSFIRQILEGLDYLHHKNILHLDIKPENILMADTTSEQIRICDFGNAQELTPGDPQYCKYGTPEFVAPEIVNQMPISTVTDIWPVGVLAYLCLTGVSPFVGENDYTTLMNIRGYTVAFEEKMFVGLTREARGFLIKVLGNEKLRPNAEESLEHPWFKTLAKGKSISTDHLKLFQSRRKWQRSLISYKSNMVMRTIPELLQDTSNHLSIAVPKNSKEASGLSSSSDSDDLEELPFVPMPLQVQFSGSRMSLNEIPTDEEQLSQPQDNGSCREDEEEGSQAVEISSTFKEQAVQQKEATVQASPLSKHAKGTLTRNRSAEVGSSSDEETSETEKREHPRRPLKKGSSLESADVPDGEIKAARRGELRRGSSADSALLLNVSTTDGDEERDRPDKGMIKAASMELPTRCRSPGRLEDRGTLRRKFGSADEEYAQRLEMMRQRLLRGGSADGKLSGLRGPLIETLSVDKKRAEQISIRSPRSDQSSLSPIPAIKLTRAASSEAAPGREATEERVLRKTSSFSHSDTEPLVLHRRSGAPLEIPLAQLEAQRLKESPSLSALTDRLESRPQTPREMIPKSLTPEAPQLKAESGEPETQSDKEAEKGTTPLIQHHKDKDSNSLKDLVIVLPRVTPPLDKMASKTELPSIQTERAQDKIPRADVNLPATTSSNETVPTKPPPLLLPSKQSLSVPPEPLPSSVLTNEMPKSKEEYSSSRAIKAKESISKTETTDQQDKIKEPLPQAVVPLPLPMPASQVLKPSTAKVQIADSPTMPENDASLPQITGKEKLSSPAVQPVDKTAAISSPYAAMIQTLQVPEEPETVVEECSKAPLKAQPVFQEIPIATGDAKAQGISQVNNKEGKDELSQEPKLINTRSSSTENVPFISNIDSEEIFEAKFKRNRENSLTRGLKKLTRTWSEEKNLAAAHASREEEMYRPSPVGVPLEFLVPAALGLHERSRSVQDLPDAERDSSFMRKLSLRFKKTPPTERKQKPAEEVEGTGRRLSWSIGRGSSKDKKDTESLNSETGSIETITDKHLKDQKKSSQSPVLAMRKKIGSTMDRLSMKLRSQSEDRRDAERTEKKEGRTDKSSPLMSLLRRSNSEGENLKKLGIPQNQLASQVSAPSTESIESGLSIKSEMVVKDDRRSRWDRWGLSRSKKEKMTSQPSIPASLMHEDGSIVGRQYIRNESDFPPVFHIKLKDIVVLEGEPVTLSCLPAGSPSPQILWKKDKMSLERGEKVHIKSCPDGRQQLIIPAAGQREAGLYECVAANALGSVASSCTVSVARIPDPPGTPEIPQKYKNTVLVLWKTHEHNSPCTYILEKQVNGDGEWKVLSSGITDCYFNVTELQEGTVRFRVACANKAGKGPYSNVSKTIVVDAEDRKPGTPSVQKARPVGAAVSTTVPMTLRTPSTPAVAPPVVSTPKLPPSCGSALAVSSTVITTTATVSPAQPLPKGPPPATPPRKHRGILSTPPTMHKPVIATTLSADITPQTLPNNRAPSSVAPPAVCSHVSTVTVSMSPGSSVTHLRAPFSTPMQKPTPEPITKPLPVSQVQSPKAPSVPTEQTQKIALAPAVAQVKAPFVPTTAKTNEPSRLPYSVPTEPQMPSQLAAPKLSAKSPSVPIMLHIPPFKSATLPASSPTSKTISFPSPPTSPVTKPLTPTSPAYMVTSFISMPVPSPSVESSTPPTPLTPITPPTPITPPTPLTPTTPPTPISPTSPTAPFTPTSQTVPTRVIVRSVTPGKDARYTPGGRVTPSGRESTTLRQGVPQKPYTFLDEKARGRFGVIRECKENATGKHFMAKIIQYEPENKQNVLQEYEILKGLHHQRILSLHEAYITPRYLVLISENCTGRELLYCLVERFRYSEDDVVNYLLQILQGLEYLHEQKVLHLDIKPENVIVSYMNTVKIIDLGSAQNFTPLVLRPLGKRIGTLEYMSPEMLKGDAVGPAADIWGVGVLTYIMLSGRSPFFELDPVETENKILSGRFDIFKLYSNASQSASLFIRKILTIYPWSRPSLKECFSNPWLQDAYLMKLRRQTLTFTTNKLKEFLVEQQRRRADTATKHKVLLRSYHGSQPSAPVTQ

Target ORF information:

RefSeq Version	XM_031892848.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis striated muscle enriched protein kinase (speg), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031892848.1

ORF Insert Sequence:

ATGGCAGGCA AAGGCCCCGC ATTCAAAGCT CCACCCACTT TCAAGATGCC TCTTTTGGAC 
CAGAAAGGGT TTGAAGGCCA AGAGATTGTT CTCAGCGTTC AGGTGGAAGG GGAACCCAAG 
CCTATTATCA ACTGGCTGAA GAACAAGCAG CAGGTGAAGC CTGGTGGAAG GTTCCGCATA 
TCAGAAGGTG CTTGGGGGAT CTTCTCGCTA CATATTGCCG GGGCTGATAA ACGAGATTCC 
GGATTCTACA CCTGTAAAGC AATAAATGAG TATGGGACAA AGCAATGTGA AGCCAAGATA 
GAGGTTCTAG CACCCCCTGG CAGTACCAGC TTGGAGGTTC TGGCCCCCTT GCAGGATGTG 
AGTGTGTGCG CTGGGGAGGC AGCGGTGTTT GAATGCGTGG TGTCGGGCCC CGCCGATATG 
GACGTGGACT GGATGTTCCG GGGCAAGCTG CTCCAGCCAG CACTGCTGGA CTGTAAGATG 
CGTTTCAACG GAAAGCAGTG TGTGCTGCTG CTGAATTCAG TCCATGAAGA TGACAGTGGG 
GTCTACACCT GCAAACTGAG CACTGCAAGA GATGAGCTGA CCTGTAGCGC TATGCTAACT 
GTGCACCCAT CGCTGGCGCC TCTCTTCACT CGCAAGTTGT CAGATCGAGA TGTTATAGAG 
GGACGCACAG CTCGGCTGGA CTGTAAAATC AGTGGCACCC CGCCCCCTGT TGTCACGTGG 
ACTCATTTTG GACAGCCCAT AGAGGAAAGT GAGAACATCC ACGTTTTGAG AGAAGGTGGA 
CATCACACCT TACTGATACC CCACGTGGCG AATGAGGACG AGGGTCAGTA CACAGCGACT 
GCCCATAACA AGCATGGGGA GGCCGAATGC TCTGCTGAAC TCTACGTGGA GGAGCCTCGT 
TCTGCTACAG CATCTCATAT CTCCAAGCTG GAGAAGATGC CATCTATTCC AGAAGAGCCA 
GAGATGCAGG AGTGTGAGGT AGAGGGTGTG ATGATGCCGG ATTTCCTGCG ACCTCTGCAG 
GACATTGACG TGGTGGAATT TCGGGAGGTA CAACTTGAGT GTCAGGTAAC CGGTCTGCCG 
TACCCTACCA TCACCTGGTA TCACAATGGT CAGAAGATCC AGAGCTCTGA TGATCGCAGA 
ATGACTCAAT ATAAAGACAT CCACCGCCTG GTTTTCCCCT CTGTTACTCA TTCTCATGCC 
GGAGTCTATA AAAGTGTCAT CTCAAACAAA GTGGGGAAAG CAGCCTGCTA TGCGCACCTG 
TATGTCACAG ATTTGGTACC CACTCCTCCA GATGGTCCCC CCATCATTAC CTCTGTAACT 
GGGAGAATTG TGAAGCTCCA GTGGAAGCCA CCCAAGAGAT TGGACCCAGC CATTGATCTG 
GCTCAGATTA CATACTCAGT CCAGCAGCAA GCGGTGGGAT CTCCCCAATG GACAGTCATC 
ACCACCAACC TAAAAGACAC TTGTTATTGT GTCAGATCTC TGACCAAGGA ACATCAGTAT 
CTTTTCCGTG TGACCACAAA CACCCCAACT TCTCACAGTA AACCTTCCCC TCCCAGTGAA 
AGCGTTTGTC TCTTTAATAG AGGTCCCTAC CTGGAGGAGA TGCCTTCTAT ACTGGATCGG 
CCTGAAATCT TGTATATGGT GGAAAATCAG CCTCTTTGCA TAACCGTGAC ACTGAACCAC 
GTACATGCCA AAGTCACGTG GCAGAGAATA GACACCGTTC TGAGCAATGT CGAGGGTGTG 
TATGAGCTGA GTATGCCGGA TGACGACCAG CACTCGCTCA CGTTGTTCAG CCCAAGGAAG 
TCTGACCTGG GGCAGCTGAC ATTTGAGGCT AAAAACCGCT ACGGATCAGA TAGCTGCACT 
ATTTCCATCG AGCTCGCAGA GGCTCCTCGC TTTGAGTCCA TCATGGAAGA CATAGAGATC 
GGTGTTGGTG AAACAGCTCG TTTTGTGGTC GTGGTGGAAG GGAAGCCTAT TCCAGACATC 
ATGTGGTACA AAGATGGGGT ACTTTTGGCA GAGAGCGGTC ACTTCAGCTT TGTGTATGAT 
GATACCGAGT GCTCTCTGGT GATCTTAAAC ACGGCACTTG GTGACTCCGG TGTGTACACC 
TGCACGGCAC GGAACCTTTC AGGGGAAATT TCCTGCAAAG CGGAGCTGCA AGTGTGCCCA 
GATGAACCAG GGGCAGGATC TGCAATACAG GAAATAGATA TACTCAAAGT TCGTAGATTG 
ACTGATTTCT ACAACATCCA TAAAGAAATT GGCAGAGGGG CCTTCTCCTA TGTCCGACAT 
GTGGTAGAAA AGAGCAACGG GCGAGATCTT GCTGCTAAAT TCATACCGTT CCGTGGGGCA 
ACCAGGGAGA GTGCACGGAG GGAGCGGGAC ATACTGTCCA AGCTCAGACA CGACAGGATC 
ATCTTCTTCT ACGATGCATT TGAGAAAAGA AGTGCTCTAA TCATTGTCAT GGAACTCTGT 
TCTCAAGAGG AGCTCCTAGA GAGGTTGACA AGAAAGCCTA CAGTGAGCGA ATCAGAGATC 
CGCTCGTTTA TACGGCAAAT CCTAGAAGGG TTAGATTATT TGCACCATAA AAACATTCTG 
CATTTGGACA TAAAGCCAGA GAACATCCTG ATGGCCGATA CGACTAGTGA ACAGATTCGC 
ATTTGTGACT TTGGAAACGC TCAGGAACTA ACCCCGGGGG ATCCTCAGTA CTGCAAATAC 
GGAACCCCGG AATTTGTGGC GCCCGAAATT GTGAACCAGA TGCCAATTTC TACAGTAACG 
GACATATGGC CAGTTGGAGT CCTGGCATAT TTATGCCTTA CCGGAGTGTC GCCCTTTGTT 
GGGGAGAATG ATTACACCAC TCTGATGAAT ATCCGGGGTT ACACTGTGGC ATTTGAAGAG 
AAGATGTTTG TTGGTCTCAC ACGAGAAGCA CGTGGTTTCC TAATCAAAGT CTTGGGCAAT 
GAGAAGCTGA GGCCTAATGC TGAAGAGAGT TTGGAGCACC CCTGGTTCAA AACTTTAGCG 
AAAGGGAAAA GCATCAGCAC AGACCATCTG AAGCTGTTCC AGTCTCGCCG TAAATGGCAG 
CGCTCACTTA TCAGCTATAA GTCCAATATG GTGATGAGAA CCATTCCAGA GCTGCTTCAG 
GACACAAGCA ATCACTTGTC CATTGCTGTA CCCAAGAATT CCAAGGAAGC ATCCGGATTG 
TCATCCTCCT CTGATTCTGA TGACCTGGAA GAGCTTCCCT TTGTCCCTAT GCCTCTCCAG 
GTCCAATTCT CTGGATCACG CATGTCCTTG AATGAGATCC CAACAGATGA GGAACAACTG 
TCTCAGCCCC AGGACAATGG CAGCTGTAGA GAAGATGAGG AAGAAGGAAG TCAGGCAGTG 
GAAATTAGTA GCACATTTAA AGAACAAGCA GTTCAGCAGA AAGAAGCAAC AGTCCAAGCA 
AGTCCATTGT CCAAGCATGC CAAAGGAACC CTTACAAGGA ACAGGTCTGC TGAAGTTGGT 
TCTTCTTCTG ATGAGGAAAC TTCAGAAACT GAAAAAAGAG AGCATCCTCG TAGGCCTCTG 
AAAAAAGGTT CCAGCTTAGA ATCTGCCGAC GTTCCTGATG GGGAAATTAA AGCAGCTCGT 
AGAGGAGAGC TGAGGAGGGG AAGCTCTGCA GATAGTGCTC TTTTGCTCAA TGTGTCTACA 
ACAGATGGAG ATGAAGAGAG AGATCGACCA GACAAGGGAA TGATAAAAGC TGCCTCAATG 
GAGCTACCAA CCAGATGCAG GAGCCCAGGT AGATTGGAGG ATCGAGGCAC ACTACGAAGA 
AAGTTTGGAT CAGCTGATGA AGAATATGCT CAGCGGTTAG AAATGATGAG ACAGAGGCTA 
TTGAGGGGAG GTTCGGCAGA TGGAAAACTC AGTGGTCTTA GAGGCCCACT CATAGAAACA 
CTTTCTGTAG ACAAGAAGCG AGCAGAGCAG ATATCTATAC GCTCCCCAAG AAGTGATCAG 
TCTTCTCTGA GTCCTATTCC CGCCATCAAA CTAACCCGAG CTGCTTCCAG TGAGGCTGCG 
CCTGGTAGAG AAGCAACTGA AGAAAGAGTC TTAAGGAAAA CCAGTTCCTT TTCTCACAGC 
GACACAGAGC CACTGGTCTT GCATCGCAGG TCAGGGGCAC CCCTAGAAAT ACCTCTTGCT 
CAGTTAGAGG CACAGCGTCT TAAAGAGTCT CCCTCTCTTT CAGCATTGAC AGACCGATTA 
GAATCACGTC CTCAAACACC GCGAGAGATG ATTCCCAAAT CTCTAACTCC AGAAGCACCG 
CAATTGAAGG CAGAAAGTGG GGAGCCAGAA ACTCAGAGTG ATAAGGAAGC TGAGAAGGGC 
ACCACACCTC TTATTCAGCA TCATAAAGAC AAAGACTCAA ATTCATTGAA GGACTTGGTT 
ATTGTTCTTC CAAGAGTGAC TCCACCTTTG GATAAAATGG CATCTAAAAC AGAGTTACCA 
AGTATTCAAA CAGAGAGGGC ACAGGACAAA ATCCCTAGAG CAGATGTGAA CCTGCCAGCT 
ACTACTTCAT CAAATGAAAC TGTGCCAACA AAGCCACCAC CCTTACTTTT GCCTTCTAAG 
CAGTCTCTTT CTGTGCCACC TGAACCTTTA CCCTCTTCAG TACTGACAAA TGAGATGCCT 
AAATCTAAAG AGGAGTACTC GTCATCAAGG GCCATTAAGG CCAAGGAATC CATATCAAAA 
ACAGAAACAA CAGACCAGCA AGATAAAATT AAAGAACCTT TACCACAAGC TGTTGTTCCA 
TTACCATTGC CTATGCCAGC CTCCCAAGTG CTAAAACCTT CCACTGCAAA AGTACAGATA 
GCAGATTCTC CAACAATGCC TGAGAATGAT GCATCACTTC CACAGATAAC AGGTAAAGAG 
AAACTATCTT CTCCAGCTGT CCAACCAGTA GATAAAACAG CAGCTATATC ATCACCTTAT 
GCTGCTATGA TTCAAACACT TCAAGTGCCC GAGGAACCTG AGACAGTTGT TGAGGAATGT 
TCAAAGGCCC CATTAAAAGC TCAGCCAGTA TTTCAAGAAA TTCCTATAGC CACTGGAGAT 
GCAAAGGCTC AAGGAATATC ACAAGTAAAC AATAAAGAAG GCAAGGATGA GCTATCACAA 
GAACCCAAGC TGATTAACAC CAGATCTTCC AGCACAGAGA ATGTCCCATT TATCAGCAAC 
ATTGATTCAG AAGAAATCTT TGAGGCTAAG TTCAAGAGGA ACCGTGAAAA TTCTCTTACC 
CGAGGTCTAA AAAAGCTTAC CAGGACTTGG TCAGAGGAGA AGAATTTAGC TGCAGCCCAT 
GCATCTCGGG AAGAAGAAAT GTACAGGCCT AGTCCTGTGG GTGTCCCACT AGAGTTCCTT 
GTTCCTGCAG CTCTTGGGCT ACATGAAAGA TCAAGATCAG TGCAAGATCT GCCAGATGCT 
GAGAGGGATT CAAGCTTTAT GAGAAAGCTT TCTTTGAGAT TTAAGAAAAC TCCACCAACT 
GAAAGGAAGC AGAAACCAGC AGAAGAAGTA GAAGGTACTG GTAGACGACT TTCATGGTCA 
ATAGGTAGAG GAAGTTCCAA AGATAAGAAG GATACAGAGA GCCTGAACTC TGAAACAGGC 
TCAATAGAAA CCATCACAGA CAAACATCTA AAGGACCAGA AAAAGAGTAG TCAATCACCA 
GTGTTGGCCA TGAGAAAGAA AATAGGCAGT ACTATGGATC GACTTTCTAT GAAACTAAGG 
AGCCAATCAG AAGATAGAAG GGATGCAGAG AGAACAGAGA AGAAAGAAGG GCGTACAGAC 
AAAAGTTCTC CATTAATGTC TTTACTTAGG AGATCCAACT CAGAAGGAGA GAACTTAAAG 
AAACTAGGTA TTCCCCAGAA CCAACTTGCT TCCCAGGTTT CAGCTCCATC AACTGAGTCT 
ATTGAATCTG GTCTCAGCAT CAAATCTGAA ATGGTAGTCA AGGATGATAG GCGCTCAAGG 
TGGGATCGTT GGGGCCTGTC ACGTAGCAAA AAGGAGAAGA TGACATCGCA GCCAAGTATT 
CCAGCCAGTC TGATGCATGA AGATGGCAGT ATTGTTGGCA GACAATACAT TCGTAATGAG 
TCAGACTTCC CCCCAGTCTT CCATATCAAG CTGAAGGACA TCGTGGTGCT AGAGGGAGAG 
CCGGTGACTC TGTCCTGCTT ACCTGCTGGC AGCCCTTCCC CCCAGATCCT GTGGAAGAAA 
GATAAGATGT CCTTGGAAAG AGGAGAAAAG GTGCATATAA AATCCTGCCC CGATGGCAGA 
CAGCAGCTCA TTATTCCCGC CGCCGGGCAG AGGGAAGCCG GTCTGTATGA ATGCGTAGCA 
GCCAATGCCC TGGGCTCAGT GGCCAGCTCC TGCACAGTGT CTGTGGCAAG AATACCTGAT 
CCCCCGGGGA CCCCAGAGAT CCCACAGAAG TATAAGAACA CGGTGCTGGT TCTATGGAAG 
ACCCACGAGC ACAACTCCCC TTGCACCTAC ATCCTGGAGA AACAGGTTAA TGGTGATGGG 
GAATGGAAGG TTCTAAGTTC TGGAATCACC GACTGTTACT TCAATGTGAC GGAGCTCCAG 
GAAGGCACTG TCAGGTTCCG CGTCGCTTGT GCCAACAAAG CAGGAAAGGG GCCCTACAGC 
AATGTGTCCA AGACAATTGT CGTAGATGCA GAAGATCGTA AGCCAGGAAC ACCCTCTGTC 
CAGAAGGCAC GGCCCGTGGG AGCTGCAGTG TCCACTACTG TTCCAATGAC TCTCCGGACC 
CCTTCTACCC CAGCTGTTGC GCCCCCTGTT GTCTCTACCC CCAAATTACC TCCATCTTGT 
GGATCTGCTC TTGCAGTTTC CTCAACAGTT ATCACTACAA CCGCAACTGT GTCTCCAGCC 
CAGCCTCTCC CCAAAGGGCC TCCACCTGCA ACTCCTCCAC GGAAACACCG AGGGATACTC 
TCCACTCCCC CAACCATGCA CAAGCCTGTC ATTGCCACCA CTCTCAGTGC CGATATTACT 
CCCCAAACTC TGCCCAATAA CAGGGCTCCA TCTTCTGTGG CTCCTCCTGC CGTATGTTCT 
CATGTCTCCA CGGTGACTGT GTCTATGTCA CCAGGTTCTT CGGTGACCCA TTTAAGGGCC 
CCCTTCTCTA CCCCCATGCA GAAACCTACC CCAGAACCAA TAACAAAACC ATTACCAGTG 
TCACAAGTAC AATCACCCAA AGCTCCTTCT GTGCCAACAG AGCAAACCCA GAAAATTGCC 
CTTGCCCCGG CTGTTGCCCA GGTTAAAGCA CCTTTTGTGC CAACTACTGC AAAAACTAAC 
GAACCATCAC GTCTTCCATA TAGTGTCCCT ACGGAACCAC AGATGCCATC ACAGTTAGCA 
GCTCCAAAAC TATCTGCCAA GTCCCCCTCT GTTCCAATTA TGCTGCACAT TCCCCCCTTT 
AAATCAGCAA CCCTACCTGC TTCCAGTCCC ACTTCCAAAA CCATATCTTT TCCCTCTCCT 
CCTACAAGCC CAGTAACCAA ACCCTTAACA CCTACCTCCC CAGCTTATAT GGTCACTTCC 
TTTATATCAA TGCCTGTGCC TTCACCCTCT GTTGAGTCCT CCACTCCACC TACTCCACTT 
ACCCCCATCA CCCCACCTAC CCCCATCACC CCACCTACCC CACTTACCCC CACCACCCCT 
CCTACCCCCA TTAGCCCCAC TTCCCCAACC GCCCCATTTA CCCCCACTTC CCAAACTGTC 
CCTACACGGG TGATAGTAAG AAGTGTCACC CCCGGGAAGG ATGCACGTTA TACACCCGGA 
GGAAGAGTCA CACCCTCGGG GAGAGAAAGC ACCACCCTCC GACAAGGGGT ACCCCAAAAA 
CCTTACACTT TCTTGGATGA GAAAGCAAGG GGGCGGTTTG GAGTGATCCG AGAGTGTAAG 
GAGAATGCAA CTGGGAAGCA CTTCATGGCA AAGATAATCC AGTACGAACC AGAAAACAAG 
CAAAACGTGC TGCAGGAGTA TGAGATCCTC AAGGGTCTCC ACCATCAACG AATCTTGAGT 
CTTCATGAAG CTTATATTAC CCCTCGCTAC CTTGTGTTGA TTTCTGAAAA CTGCACAGGA 
CGGGAACTGC TTTACTGCCT AGTTGAGAGG TTCCGTTATT CTGAAGACGA CGTGGTCAAT 
TACCTCCTGC AGATCCTACA GGGGTTGGAG TACCTCCATG AACAAAAGGT CCTGCACCTC 
GATATCAAAC CTGAGAACGT CATTGTGTCC TACATGAACA CTGTCAAGAT CATCGATTTG 
GGGAGTGCCC AGAACTTTAC ACCGCTGGTC CTGCGACCCC TCGGCAAACG CATAGGGACC 
CTGGAATATA TGTCCCCCGA AATGCTCAAA GGTGACGCAG TAGGCCCAGC AGCTGATATT 
TGGGGTGTAG GCGTTCTGAC TTACATAATG CTCAGCGGCC GCTCTCCGTT CTTTGAGCTG 
GACCCCGTGG AAACCGAGAA CAAGATCTTG TCGGGACGAT TTGATATCTT TAAATTGTAT 
TCCAACGCCT CCCAAAGTGC TTCCCTCTTC ATTCGCAAGA TCCTGACTAT ATATCCTTGG 
AGCCGCCCAT CACTTAAGGA ATGTTTTTCC AATCCCTGGC TCCAGGATGC CTACCTAATG 
AAGCTTAGAC GCCAAACCCT AACCTTCACC ACTAACAAGC TCAAAGAGTT CCTCGTGGAG 
CAACAGAGGA GACGCGCAGA CACAGCCACT AAGCACAAAG TCCTCCTTCG TTCGTACCAC 
GGAAGCCAGC CCTCAGCTCC TGTCACTCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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speg cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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