spegb gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_009304895.2	XP_009303170.1	striated muscle preferentially expressed protein kinase
XM_021479009.1	XP_021334684.1	striated muscle preferentially expressed protein kinase isoform X4
XM_021479006.1	XP_021334681.1	striated muscle preferentially expressed protein kinase isoform X1
XM_009304895.3	XP_009303170.2	striated muscle preferentially expressed protein kinase isoform X5
XM_009304896.2	XP_009303171.1	striated muscle preferentially expressed protein kinase
XM_021479008.1	XP_021334683.1	striated muscle preferentially expressed protein kinase isoform X3
XM_021479010.1	XP_021334685.1	striated muscle preferentially expressed protein kinase isoform X5
XM_021479011.1	XP_021334686.1	striated muscle preferentially expressed protein kinase isoform X6
XM_021479007.1	XP_021334682.1	striated muscle preferentially expressed protein kinase isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa144102	XM_021479006.1 Latest version!	Danio rerio SPEG complex locus b (spegb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa144103	XM_021479007.1 Latest version!	Danio rerio SPEG complex locus b (spegb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa144104	XM_021479008.1 Latest version!	Danio rerio SPEG complex locus b (spegb), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa144105	XM_021479009.1 Latest version!	Danio rerio SPEG complex locus b (spegb), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa144106	XM_009304895.3 Latest version!	Danio rerio SPEG complex locus b (spegb), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa144106	XM_021479010.1 Latest version!	Danio rerio SPEG complex locus b (spegb), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa43992	XM_009304895.2 Latest version!	Danio rerio SPEG complex locus b (spegb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa43992	XM_009304896.2 Latest version!	Danio rerio SPEG complex locus b (spegb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa43993	XM_021479011.1 Latest version!	Danio rerio SPEG complex locus b (spegb), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

spegb ( XM_021479006.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334681.1 Danio rerio SPEG complex locus b (spegb), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa144102
Clone ID Related Accession (Same CDS sequence)	XM_021479006.1
Accession Version	XM_021479006.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10827bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	striated muscle preferentially expressed protein kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAAGG CTGAGGTACA GATGACCCTG AAGAAGGGTG CTGATGAAGA AGCTGGACGA 
ATGGCTCCTC CAAGCCCCAG GATACCATCG AAACGGGCAA AAGTTAGTTC TGAGCAGGTC 
GTCCCAGATC CCTCCGCATC CGTCTACCCA ACTCCAACTC CTCCAGTGTT CATCCGGAAG 
ATGAGGAACG CTGCGGTGGG TACTGGCTGC GATATCCGTT TGAAAGTGGC CGTGGCTGGA 
GACCCACAGC CTACTTTGTA CTGGTACCAC AACGATGACC TGGTCAACAT GGACAACCAG 
GAATATGGAG GCCTGTGGAT CAGGGACTGT AAACCCAGTG ATGCCGGCCT TTATACCTGC 
ATCGCCACCA ACCATCTTGG AGAAGCCAGA AGCAGTGCAG TCCTAGCTGT ATTGGATTTG 
GGTGAAGAAA ACCGGACACT CTACCCGAAC ATTTGGGCGG TTCACTCATG CGAAGTTTTC 
CGCTCCTGTC GGAAACAAAG TTATGACTCT GAGACTACAG AAGACGAAGC CGCTGAGCCA 
CAAATACCCC TGGAGGCCAA AGAAGGAGCC AGAGGTTTTC AGGATGAGGC CCAACGAGTG 
CCTGCAGGTG AGAGAATGAT GGATGTCGCC CCAGATGCGA GCAATCGCTG GGCCGCCAGT 
GAAAGCACAG TGTTGGAGAG AGAAGCTCTA GCCCTGGGAT CGAGAGCCCC AGGGCCGCAG 
TCTGCCAGGA GCCCATCTGA CGATGTTTTG AGGGATTCTC CCCCTCAAGT TCTACCACCC 
CCTTCCCCCA AATTTGGCCG CTCTACTGCC TCTCCTTTGC TCAGCCGTTC TGGTTCGGGC 
CCTGCCACAC CCCTTGCCCC TCGCAAGAAA GTTGTCGTGC CAACTGAATA TCAAGACACA 
GTACCAGGAG AGTTTGAGGA GAAGATTAAG CAACCCAAAT CCTCTGGGAT GTCTCAGAGT 
AGTGCTCAAG ACTCTCGACC CCAAACCCCT GTCAGTGAGT ATTCCCGTAA GGAGCTTACA 
CTTCGGCCCT CACCAAAACT GACAAGAGCT AGCTCCAAAA TCTTTGAGAA GGTGCGAGTC 
TTCGAAGAGC GCAGAAGAAG CATTGATAAC CCTGAAGGAT CTATATCTGG ACGTTCTTGG 
GCTGGATTTA ACAGAGCTTC ATCTATCGAC TCTGATGAAG GAGGGAGCAG GTTGGGCATC 
TCCAGGGAGA GCTCCAAAGA AGACCTTCGT GAGGCTTTGA AGGCAGATGC AGCCCAACGA 
AGGACCATGT TTAAACAGCG GGCTGCTTCC CTGGAGGACA GACCTCGTTA CACCCAGAAG 
GTGCAGGACA TTGAAAACAA GTTCACAGAG GAGCTCCAGC GCATCAAAAA GCTTGTCGGC 
AAACCACACA TGAAGAAATC CTTCTCAACA GAGCAGCTCT CTACTTGTCA CAGAAGCAGG 
CAGCCTGTAA GAAAGTTAGA GCCCATCCCA CCTCAGGTCC TTCAAAAGCT GCAGGATAGA 
GAACGTGCTC AGCTAGCTCA GCTAGAGCAA GAGATGAGAG AGCGAGACCA GGCCAAAGAA 
CGATCACCTC CGAAATCACC GTCTCGCAGA AGGAAAGACC ATCTGGCACA ACAACCACCA 
GAAAGAGCTG AGACCAAGGT GGTAAGCACA ACTGGACTTG AACCATCCCC TCCAGAAGCC 
ATGTCACTCT CTGATTTACC AGGTCAAAGA TCTCCAAGAT TTCGGGGCCC AAGCCCAAGC 
AGAGATAGTA CACGAAGGTC TCCAACCGTA GAGATTAGTG CCATGGCTGA GGAGCGCCCT 
CGAGGAAGAG CTGAATCTCC TTCAAGAAAT GTTTTGGAGA TGACGTTACG CAAAGTGGAG 
CCAAGACCTG CAAGTCCACT GGTAAAGAGG GTGGTTCGTG TTCAAGAAGT TCCACAAGCA 
AACGATCAGC CTATGCATAG AAAGACGCCA ATAGAGGTCT CGCTAAGAAA ACTGGAAAGA 
AGGCCTGAAA GTCCCCTGGT GCAAGGAGAA ACCGTAGCCA TTCAAGACTT CCCTGTTCAA 
GCTCCCCCCA AACCTCCAAG AGTTTCCTCA TCTTCCTCGT CTGAGGAGAA GATGGAGCTG 
GAAGTGACTC CTCCACCACC CCCACTAAAA CTCACTATAC CAAAAATTAT TGTTGAAGAA 
GAGCCCATGG AGACAGACAC ACCTGTTGAG AAGACTGAAA AGGCTGTGAA AGTTGGCACT 
GGTAAAGATG AAAAACATCA AAGATCCAGA GGAAGAGGAC GCAGACAAAG ACCAATGTCC 
CCTGAATTAG AATCTTCGGA CGACTCCTAT GTGTCTGCAG GAGAGGATCC ACTAGAAGCT 
CCTGTGTTCG AGTTTCCACT TCAGGACACT GTGGCCTCCA CTGGAACGGA GGTGCTATTA 
AAATGCATCA TCAGTGGCAC CCCTCTCCCT GAAGTTATAT GGAAGAAAGA CAACACTGAG 
GTCAAGAACA GTCCCACTCA TGTTGTAAAG ATAGAGGGGG AACGCCACTC CTTGCTCATC 
AAATGGACAA AGCCAAGTGA TGCAGGAACC TACACAGTTA CCGCTGTCAA TGAGGTCGGT 
GAGGTGTCCA GCAGTGCTAC ACTTTTCATC AAACCAGAGC CCAGTCAGGA CCATCGTGGT 
AACCTTGGTG TCCCTATGGA TGTCAGCAGT CCCATCACAT CTGATGAGGA GTATCTCAGT 
CCACTGGAAG AGAGCATGGA CTTCTCCTCC TACAGGGGAC CGGAGCCAAG AAAGATCGTG 
GACACTCGAT TTAAGGAGCC TCCAGCCTTT CTGGCTGTTA TTGGAGACCA AAGCGTAATA 
GAGGGTCAGG AGGTGACCAT GTCTGTTCTA GTGACCGGAC AACCTAAACC TATGCTTTAC 
TGGCTGAGAG ACAGAGTGAC CATTAAAACC GGAACACGGC ATATCGTCCA AGAGACAGAG 
GAGGGCAACT TTGAAATGAT AATTAAATCA GCACAGAGGT CAGACACCGG TGTTTACACC 
TGCAAGATCA TCAATGAATA TGGAACTAAG CAATGTGAAG GCAAACTGGA GGTGAAAGCT 
CGTCCAGTAG AGCCAGGTCT GGCCATCATT CGGCCAGTGA GGGACGTGAT GGTGAAGGCA 
GGGGAGACCG CTCTGTTTGA GTGTCATGTG ATCGGCCCGC AGGACACAGA TGTTGACTGG 
CTGTCAGATG GGAAACTCAT CCAGCCTGCT TTGTTGAACT GCAAGATGCA TTTTGATGGG 
AAAAGGTGCA GGCTGCTTCT AAACTCTGTA CATGAGGATG ACAGTGGAAC GTACACCTGC 
AAACTGAGCA CAGCTAAAGA GGAACTGACC TCCAGTGCCA AGCTAAAAGT CATAGCATCC 
ATAGAGCCCC TTTTCACTCG TAAGCTGGAT GTGCTGGAAG TTATCGAGGG TCGTAATGCA 
CGATTTGACT GCAAGGTCAG CGGGACGCCG CCGCCTCAAG TCATATGGAG CCACTTCGAT 
CGTCCGCTCG AGGAAAACGA AGATATCAGG ATCTTAAAAG AAGGAGGACG GCACTCATTA 
ATCATCTCTC ATGTTAGTAA TGAAGATGAG GGCCTCTACA CGGTTGCAGC GCGAAACTCA 
CACGGGGAAG ACGAATGTGC AGCGGAGCTC TATGTGCAGG AGCCCAGACC AGCAATATCT 
TCTCAAATAG CGAAGCTGGA AAAAATGCCT TCCATTCCCG AAGAACCCGA AGTTCCTGAA 
AATGAGATTG AGCGTTTCAC CATGCCTGAC TTCGTAAAGC CGCTTTATGA TATGGATGTA 
GTGGAGGGCA GAGAGGCTGT GCTCAGGTGC AAAGTGGCCG GCTTGCCGTA CCCCACCATA 
ACATGGTTTC ACAATGGCAA GAGAATTGAC AGCACGGAGG ACAGAAAGAT GACACAGTTC 
AGAGATGTTC ACAGCCTGGT GGTCCGCTGT GTTTGTCATG CTCACGGAGG GGTTTACAAG 
TGTGTCATAT CCAATAAAGT GGGCAAGGCC ACCTGCTATT CTCATCTTTA CGTGACAGAC 
ATCCTTCCTG ACCCTCCAGA TGGTGCCCCA GTCGTTGAGT CCATCACTGG CAAGACTATC 
ACGCTAAGCT GGAAGAAACC CAAAAGACTG GATCCGTCCA TTGACCCCAG CTCCCTGATG 
TATGCCATTC AGCAACAGGC CCTGGGGTCG ATCCAGTGGA CCATCATCGC TTCTTGCCTG 
AAACAGACCA CCTATACCAT CAGCAATCTG TCTAAAGGTG TCCGCTACGC CTTCAGGGTG 
CTATCAATCA CCAGCAAGGC CTTTAGCAAG CCGTCGCCTG CCACCGAGCC AATGCAGCTG 
CTAGATAGAG GTCCGTACGT CCAGGAGGCT CCGGTAATCA TTGACAAGCC AGATATCGTG 
TATATGGTGG AGAAGCAGCC CTTGAGCATC ACAGTCACTC TCAACCACGT CAACGCATCG 
GTCACATGGA AGAGAGGAGG AGTGACATTA TCTAACCGAC CGGGTCTTTT TGAGTTGAGC 
ATGCCTGATG ATGACCAGCA CACACTGAAG CTGTGTAAGG TGAAGAGCAG TGATGTTGGC 
CAGCTCACCT GCATTGCCAA CAACAAGTAC GGCAGCGACT CCTGTGTCCT CACCCTGGAG 
ATGGCAGTGG CACCAACTTT TGAGACAATT ATGGAAGACC TGGATGTGAA TGTAGGCGAG 
ACTCCTCGAT TTGCAGTGGT GGTTGAAGGA AAGCCCGTCC CAGACATCCT CTGGTACAAG 
GGAGACACCC TTCTGTCCGA AAGCAGCCAT TTCACATTTG TGTATGATGA CAATGAATGT 
TCTCTAGTGG TGCTCAACAC TCAAGCAGAC GACTCAGGAG TGTACACTTG TACTGCGAAG 
AATCTGGCCG GTTCTGTGTC CTGCAAGGCT GAGCTGACCG TCCACACCAC CAAACAGTAC 
AAAGACGACC CGATGGAGGA TGAGGCGTCC ATCCTCAGAA AGATGCGGAG GCTCACTGAT 
TACTATGATG TGCACAAGGA AATTGGGAGA GGGGCTTTCT CATATGTGAA GCGGGTGATA 
CAGAAGGCAG GAAAGCTGGA ATACGCAGCC AAGTTTATTT CTGCTCGGGC TAAACGAAAG 
GCATCCGCTC TGAGAGAACT GAACATCCTC TCTCATCTGG ACCATGAGAG GATCCTCTAT 
TTTCATGATG CCTTTGAGAA GAAGAATGCT GTCATCATCA TTACAGAGCT ATGCCATGAG 
GAGCTTCTCG ATAGGCTTAC AAAGAAATCA ACCATCTTGG AGTCCGAGAT CCGATCCAGC 
GTGAGGCAGC TGTTGGAGGG TATTAACTAT CTCCATCAGC TTGACATACT TCATCTAGAC 
ATCAAGCCTG ATAATATCCT CATGGCAGAC CACAGTAGCG ATCAGATCCG CATATGTGAT 
TTTGGAAATG CTGTAAAGTT CATGCCTGAT GAGGCACAGT ACTGCAAATA TGGCACTCCA 
GAATTTGTCG CCCCAGAGAT TGTTAACCAG ACACCCGTCT CCAAGGCAAC GGACATCTGG 
CCCATTGGTG TTCTGACATA CTTATGTCTT ACTGGAGTTT CTCCTTTTGC TGGGGAAAAC 
GACCGCAGCT CTGTTCTCAA CATCAGGAAT TATAATGTGG CTTTTGAAGA AAGCATGTTT 
ACAGACCTCT GCCATGAAGC CAAGGGATTC GTCATCAAGC TGCTGGTGGC TGATAGATTG 
AGGCCTGATG CTAATGAATG TCTTCGCCAC CCCTGGTTCA AGACCCTCAA CAAGGGTAAA 
AGCATCAGTA CAGAGTCCCT TAAAAAGTTT TTGTCCAGAA GAAAATGGCA GCGATCGCTT 
ATCAGCTACA AATCTAAAAT GGTGATGAGG TCTGTACCTG AGCTACTGGA TGACTCCTCC 
AGCCATATTT CCATTGCTGT TCCGCGACAT CTGAAAAAAG GCTCACCACC ACTTTCATCA 
TCTTCAGATT CAGATGAAGA CATCGATGAG TTGCCTTTTA TTCCCATGCC ACTCAACGTG 
GAATTCTCAG GATCTAGAAT GTCACTGACC GAAATTGTAG GTGATGATGA CATGACAGGT 
AAATTGAATG GAAATGCTGA GAGGCCTGCT TCACTCCATC CGAAGTCCGA GCCAATGGAA 
GTGGAGATGT CAGAGAGAGA AGGTGGAGAT CCATCTGCTA GGGGTAGAGC CAAGAAGAGG 
ACTACAACAG AAGATGAAAA AGGGTCGTCT GATGAGGAAC CATCACAACT GGCCAAAAGA 
GCAGAACTGG CAAAGAGACC AATGCGCAGA GGTTCAAGCA TGGAGTCAGA TGAGCCAGAG 
GGAGGACGTC GCAGAGGAGA GTTACGCAGA GGGAGTTCGG CTGACAGTGC CTTACAGCTC 
CATATCAAAC CAGAGGAGGG CACAGAAGAA AGTCCTACTG ATGGAAGTAG AATTCTACAA 
AAGTCTGTAT CTATGGAGCT TCCAAGGAGA AGCCCAAGTC CAGGTGCTGC AAAGCTTAGC 
CAGGAAGACT ATGCTCTGAA ACTGGAACTG ATGAGGCAGC GACTACTCAG GGGTGGCTCT 
GCTGACAACA AGATGAGTGG ATTACGAGGC CCATTGCTGG AGACACTAGG TATGGGAGAT 
GAGAAGCGGT TTGTGCGGGG ATCTCGTCTA GGTAACCCTC CACTTGTTAG AGCGGCATCT 
AGTGAGTCAC CTAGAGATGA CATCCCAAAG ACAAAGATTC TCCGCAAGAG TGCTTCCTTT 
AGCCAGGGGG ATTCAGAGCC CATGCCCCTG CACAGAAGAA TCGGAGCACC TCTAGAGATT 
CCTCTAGCAC AAATAGAGGA GAGACGCCTA CAGGAAGCAA TATCCATGTC TGCTCTCACA 
GAGCCTGTCA AAGATTCTCG CCCAATCACT CCAAAACTTC AAATGCCTGA AGAAGAAAGA 
CATGAAAAGA AGAAAACTGA AGAAAAAGAA TCTGTCTACA AGGACAATAA ACCTGAAGAG 
GTTATTGCTG TGTCAAAGAC TGAGGAAGAA GGAAGACGTG TTGATCAGAA ATCTTGGGAT 
TCTGATGCAC AGAAAAAGGA CCAGAGCAAG TCAGAGGAAA CCAGGGAAGA GTCTGAAAAA 
GTGGCTGAAA TTGAAAAAAA GACCTCTATA GTGGACGAAA CAATTGAGGA AGAGGAAACA 
AGAAAAGATG AGGAGCCAGA GAAAGAAACT GAGCCTCCTA CTCCAAAGAT TGAGGTTAAG 
GAATATGAGG GTGATGTAAA GGTATTACCA AAGTCTCCAG AGTTTATCAT TTCCACAAAC 
TCAATCATGG TGACCCCAAC AGCTGCCCCA GCATCCAGTC CCCAGAGTGT ATCAACCTAT 
GTTACCCCAT CTCCCCCTGC ACGCACAGTG CTCCCAGATG GTAGGATGTC CGCATATGCC 
AGTATTATGC AGACTATCAT GGTACCATCT GTCCCAGCAA ATACCCAATC TGACACTGTT 
CCTATTCCTA CACCAACTTC CACTCCACCA TCAGTTCCAG TCTATGCACC CATTTCCAGA 
CACATCTCAG AACCAACCCT TTCATCAACT TCTTCATCCA AATCAGATCT TCACCAAACA 
ACTGAGCATC CTGCAGTCTT TTCTCGGGTT GCCTCCACTG AACACCCTCC CAAAGAGCCA 
AGTCCTCCTA AGACACCCAC TCGCGCCTCC CCTAAGCGAG AGCCATCTCC TGGAAGCAAC 
ATCCAGGACC TTGCATCCGA GGAGGTTTTT GAAGCTCGCT TCAAGAAACG TGAGTCTTCT 
CTAACTCGTA GTCTCAAACG TTTCTCATTT CAGAAGACTG AAGACAAGCC CTCCACTGTT 
TCTCCTGAAA CTGCTGAAGA AATGTATCGC CCTGGACCAA CTGGTGCTCC TTTAGAGTTT 
GTGCCAAGGA GGTTGGAGGA GAAGTCCAAA TCAGTACAAG ATCTTCGAGA GGCAGAGAAG 
GACCCTGGGT TCATGAGGCG ACTCTCCATG CGCTTGAAAA GGACCCCATC AGTGGAAAAG 
CAGGAGGAGA AGCTCAAAGA GGAGGAGGCT TCAGCACCCA GGCGTCGTCT TTCTTGGGCA 
CTGGGACGGA GGGGATCCCA AGAGAAGAAG GAGATTGAGA TGATGAGGTT GGATGGGGGT 
CCTGAGCCTC CCCCAGAGCC TGAGTCTAAA GCACCTTCTG AGTCACCTAT TCTTGCCATG 
CGCAAGAAGA TTGGCAGCAC CATGGCAAGC TTATCTACAA GAATTAGGAG CCATTCTGAG 
GACAGGAAAG ATGACAGTGA AGGCAAGATG GAGACCAAGA GGACTCCTAT ATTCAACAAA 
CTAAGGCGTT CCACTTCTGA GGGGGGCAGT CTGAAGAGAA TGGGTATCCC ACAAAACCAA 
CTGGCTGCTC AGTCAGGGCA AGGGACCTCA TCAGAATCTC TTGATTCTAT GTCAAGCATC 
CAGTCAGAAT CAGCTATTAA AAATGCAGAA CTTGACCGCA GATCACGGTG GGACCGATGG 
GGCTTGACTC GAGGGAAAAA GGACAAGACA GTTTCCCAAC CAGACATCCC AGCTTCCATT 
GCTAGAGAGA ATGGTTCTCT ACGGTCTCGC CAATATACAC GTCTAACATC TGACTTCCCT 
CCGGTGTTCC ATATCAAACT GAGGGACCAT GTACTCTTGG AGGGAGATCC TGTCACTCTC 
AGCTGCCTCC CTGCTGGCAG TCCTCACCCA CACATCTCAT GGATGAAAGA CAAGAAGCCA 
CTTGAGATTG ACCCCAGGAT GAATATGATA TCTTGCCCCG ATGGGAGACA ACTGCTGATG 
ATTATGAAGA CCACCAAGAA GGATGCAGGC CTCTATGAAT GTGTGGCTGC AAACCCACTG 
GCCACAGTCA CCAGCTCATG CGTTGTGTCC CTCGCTCGTC TGCCTAATAG TCCTGGGACA 
CCCGAGGTTC CTCAGAAGTA TAAAAACACT GCTCTAGTGG TATGGAGACC CTCAGACACT 
ACGGCACCCT GCACCTACTC TCTGGAGAGG AAGACAGAGG GTGAGAATAA CTGGCTGATA 
GTCGCCACAG GTGTTGCTGA CTGTTACTAT AACGTGTTGG ATCTTCCGGC CGGCGCTTCA 
TACAGGTTCA GAGTGGCCTG CGTCAATAAG GCTGGTCAGG GACCTTACAG CAGCTTGTCT 
GAAGTAGTGG TCCTGGACTC AACAGAGCCC CAGAAGTCCA GTGGCACAGT TGTAGTCAAA 
ACGCTTTCAT CAGCTCCCGC TCCTGCAGTG GTAACGTCCA CCATGACCCT TCCTGCAATG 
AAGCCTGCTG CAATAAAACC AGCACCATCA ACAAATTCAG CGCCCTCTAC AGTCCAACCG 
GTAAAGCCTG CGCCTCCTCC TGCATCATCA ACACCTACCT ATCGTCCCGT GCCTGACATT 
CGAACTCCAT CGCCTGGCAC TCCAACCCCT GCCAAAGCCA AAAGCACCCT CAACATTACT 
ATGGCCAAAC CCTCCCCAGC ATCCCCTGCA GCTCAACCAC AACCACAACC TCCACCCAAA 
CCCTCGCCTC CGGTCATTCT GCCCAAACCT CAGACCCCAG TCAGTGTGGT TTCACCTCCA 
TCCCAAACTC CTCCAGTGTC ACCTCCACCT CTAGTGTCTC CTCCTCCCAT TATTGGTAAA 
CCAATCTCCT CAGTGCCCAT GTATGTCCCT GCTACAACCA CCTCGACTCC AGTGACTCCG 
GTCACTCCTG TCACCCCAGT CACTCCAGTC ACCCCAAATA CGCCCTCTCC TGCCCTGTCG 
CCTCCAGTGG TGTTGGTTAC AAGTCTGAGT CCTGTAGGGG AGGGAACTGG GACACCCAAT 
CGAATGACCC CTAGTGGAAG AGCCACGCCT TCTGGACGCA TCACTCCATC AGGCCGAAGG 
ACCCCTATTG GAAAGCCTGG AGACTCAACG CTGAGGCAAG GAGTCCCTCA GAAACCTTAC 
ACCTTCATGG ATGAGAAAGC AAGGGGTCGC TTTGGTGTGA TCAGAGAGTG TCGAGAAAAT 
GCCACTGGTA ACCTGTACAT GGCTAAGATT GTGCCATATG AGCCAGAGAG CAAGCAGACT 
GTATTACAGG AATACGACAT CCTGAAGTCT CTCCATCATG AGAAGATCAT GGCTCTCCAC 
GAGGCCTACG TCACTCCACG CTACCTGGTG CTCATCTCTG AGTGCTGCTC TGGCAAAGAA 
CTACTCCACA GTCTCATTGA CAGGTTCCGA TACTCTGAGG ATGATGTGGT TGCATATATC 
GTACAGATCC TTCAGGGTCT GGACTACCTG CACAGCCGGA GGATCCTGCA TCTCGACATC 
AAACCAGAAA ACATCATCGT CACTTACATG AATGTGGTGA AGATTATCGA TTTTGGTAGT 
GCACAGACCT TCAATCCTCT GTTCCTGAAG CAGTTCTCCC CACCTATTGG AACACTGGAT 
TATATGTCTC CAGAGATGCT GAAAGGAGAT GTGGTGGGTC CACCAGCTGA CATCTGGAGC 
ATTGGCATAT TGACTTATAT CATGCTTAGC GGGAGGTTGC CGTTCACAGA AAATGACCCT 
GCCGAGACCG AGGCCAGAAT ACAGGCAGCC AAGTTTGACC TCAGTAAGCT TTACCAAAAC 
GTGTCCCAAA GTGCCTCTCT GTTCATCAAA AAGATCCTGT GCAGCTACCC TTGGGCCCGT 
CCGACCATCA AGGACTGCTT TACCAACTCG TGGCTGCAGG ACGCATATCT GATGCGCCTG 
CGAAGACAGA CTCTCACATT CACCACCACA CGCCTCAAAG AGTTCCTGGA GCAGCAGCAG 
CACATCAGAG CCCAAGAGGC AACAAAGCAC AAAGTCCTCC TGCGTTCCTA CCAGAGCAAC 
CCACAAACCC CATCCACCCC TCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334681.1
CDS	712..11538
Translation	MRKAEVQMTLKKGADEEAGRMAPPSPRIPSKRAKVSSEQVVPDPSASVYPTPTPPVFIRKMRNAAVGTGCDIRLKVAVAGDPQPTLYWYHNDDLVNMDNQEYGGLWIRDCKPSDAGLYTCIATNHLGEARSSAVLAVLDLGEENRTLYPNIWAVHSCEVFRSCRKQSYDSETTEDEAAEPQIPLEAKEGARGFQDEAQRVPAGERMMDVAPDASNRWAASESTVLEREALALGSRAPGPQSARSPSDDVLRDSPPQVLPPPSPKFGRSTASPLLSRSGSGPATPLAPRKKVVVPTEYQDTVPGEFEEKIKQPKSSGMSQSSAQDSRPQTPVSEYSRKELTLRPSPKLTRASSKIFEKVRVFEERRRSIDNPEGSISGRSWAGFNRASSIDSDEGGSRLGISRESSKEDLREALKADAAQRRTMFKQRAASLEDRPRYTQKVQDIENKFTEELQRIKKLVGKPHMKKSFSTEQLSTCHRSRQPVRKLEPIPPQVLQKLQDRERAQLAQLEQEMRERDQAKERSPPKSPSRRRKDHLAQQPPERAETKVVSTTGLEPSPPEAMSLSDLPGQRSPRFRGPSPSRDSTRRSPTVEISAMAEERPRGRAESPSRNVLEMTLRKVEPRPASPLVKRVVRVQEVPQANDQPMHRKTPIEVSLRKLERRPESPLVQGETVAIQDFPVQAPPKPPRVSSSSSSEEKMELEVTPPPPPLKLTIPKIIVEEEPMETDTPVEKTEKAVKVGTGKDEKHQRSRGRGRRQRPMSPELESSDDSYVSAGEDPLEAPVFEFPLQDTVASTGTEVLLKCIISGTPLPEVIWKKDNTEVKNSPTHVVKIEGERHSLLIKWTKPSDAGTYTVTAVNEVGEVSSSATLFIKPEPSQDHRGNLGVPMDVSSPITSDEEYLSPLEESMDFSSYRGPEPRKIVDTRFKEPPAFLAVIGDQSVIEGQEVTMSVLVTGQPKPMLYWLRDRVTIKTGTRHIVQETEEGNFEMIIKSAQRSDTGVYTCKIINEYGTKQCEGKLEVKARPVEPGLAIIRPVRDVMVKAGETALFECHVIGPQDTDVDWLSDGKLIQPALLNCKMHFDGKRCRLLLNSVHEDDSGTYTCKLSTAKEELTSSAKLKVIASIEPLFTRKLDVLEVIEGRNARFDCKVSGTPPPQVIWSHFDRPLEENEDIRILKEGGRHSLIISHVSNEDEGLYTVAARNSHGEDECAAELYVQEPRPAISSQIAKLEKMPSIPEEPEVPENEIERFTMPDFVKPLYDMDVVEGREAVLRCKVAGLPYPTITWFHNGKRIDSTEDRKMTQFRDVHSLVVRCVCHAHGGVYKCVISNKVGKATCYSHLYVTDILPDPPDGAPVVESITGKTITLSWKKPKRLDPSIDPSSLMYAIQQQALGSIQWTIIASCLKQTTYTISNLSKGVRYAFRVLSITSKAFSKPSPATEPMQLLDRGPYVQEAPVIIDKPDIVYMVEKQPLSITVTLNHVNASVTWKRGGVTLSNRPGLFELSMPDDDQHTLKLCKVKSSDVGQLTCIANNKYGSDSCVLTLEMAVAPTFETIMEDLDVNVGETPRFAVVVEGKPVPDILWYKGDTLLSESSHFTFVYDDNECSLVVLNTQADDSGVYTCTAKNLAGSVSCKAELTVHTTKQYKDDPMEDEASILRKMRRLTDYYDVHKEIGRGAFSYVKRVIQKAGKLEYAAKFISARAKRKASALRELNILSHLDHERILYFHDAFEKKNAVIIITELCHEELLDRLTKKSTILESEIRSSVRQLLEGINYLHQLDILHLDIKPDNILMADHSSDQIRICDFGNAVKFMPDEAQYCKYGTPEFVAPEIVNQTPVSKATDIWPIGVLTYLCLTGVSPFAGENDRSSVLNIRNYNVAFEESMFTDLCHEAKGFVIKLLVADRLRPDANECLRHPWFKTLNKGKSISTESLKKFLSRRKWQRSLISYKSKMVMRSVPELLDDSSSHISIAVPRHLKKGSPPLSSSSDSDEDIDELPFIPMPLNVEFSGSRMSLTEIVGDDDMTGKLNGNAERPASLHPKSEPMEVEMSEREGGDPSARGRAKKRTTTEDEKGSSDEEPSQLAKRAELAKRPMRRGSSMESDEPEGGRRRGELRRGSSADSALQLHIKPEEGTEESPTDGSRILQKSVSMELPRRSPSPGAAKLSQEDYALKLELMRQRLLRGGSADNKMSGLRGPLLETLGMGDEKRFVRGSRLGNPPLVRAASSESPRDDIPKTKILRKSASFSQGDSEPMPLHRRIGAPLEIPLAQIEERRLQEAISMSALTEPVKDSRPITPKLQMPEEERHEKKKTEEKESVYKDNKPEEVIAVSKTEEEGRRVDQKSWDSDAQKKDQSKSEETREESEKVAEIEKKTSIVDETIEEEETRKDEEPEKETEPPTPKIEVKEYEGDVKVLPKSPEFIISTNSIMVTPTAAPASSPQSVSTYVTPSPPARTVLPDGRMSAYASIMQTIMVPSVPANTQSDTVPIPTPTSTPPSVPVYAPISRHISEPTLSSTSSSKSDLHQTTEHPAVFSRVASTEHPPKEPSPPKTPTRASPKREPSPGSNIQDLASEEVFEARFKKRESSLTRSLKRFSFQKTEDKPSTVSPETAEEMYRPGPTGAPLEFVPRRLEEKSKSVQDLREAEKDPGFMRRLSMRLKRTPSVEKQEEKLKEEEASAPRRRLSWALGRRGSQEKKEIEMMRLDGGPEPPPEPESKAPSESPILAMRKKIGSTMASLSTRIRSHSEDRKDDSEGKMETKRTPIFNKLRRSTSEGGSLKRMGIPQNQLAAQSGQGTSSESLDSMSSIQSESAIKNAELDRRSRWDRWGLTRGKKDKTVSQPDIPASIARENGSLRSRQYTRLTSDFPPVFHIKLRDHVLLEGDPVTLSCLPAGSPHPHISWMKDKKPLEIDPRMNMISCPDGRQLLMIMKTTKKDAGLYECVAANPLATVTSSCVVSLARLPNSPGTPEVPQKYKNTALVVWRPSDTTAPCTYSLERKTEGENNWLIVATGVADCYYNVLDLPAGASYRFRVACVNKAGQGPYSSLSEVVVLDSTEPQKSSGTVVVKTLSSAPAPAVVTSTMTLPAMKPAAIKPAPSTNSAPSTVQPVKPAPPPASSTPTYRPVPDIRTPSPGTPTPAKAKSTLNITMAKPSPASPAAQPQPQPPPKPSPPVILPKPQTPVSVVSPPSQTPPVSPPPLVSPPPIIGKPISSVPMYVPATTTSTPVTPVTPVTPVTPVTPNTPSPALSPPVVLVTSLSPVGEGTGTPNRMTPSGRATPSGRITPSGRRTPIGKPGDSTLRQGVPQKPYTFMDEKARGRFGVIRECRENATGNLYMAKIVPYEPESKQTVLQEYDILKSLHHEKIMALHEAYVTPRYLVLISECCSGKELLHSLIDRFRYSEDDVVAYIVQILQGLDYLHSRRILHLDIKPENIIVTYMNVVKIIDFGSAQTFNPLFLKQFSPPIGTLDYMSPEMLKGDVVGPPADIWSIGILTYIMLSGRLPFTENDPAETEARIQAAKFDLSKLYQNVSQSASLFIKKILCSYPWARPTIKDCFTNSWLQDAYLMRLRRQTLTFTTTRLKEFLEQQQHIRAQEATKHKVLLRSYQSNPQTPSTPQ

Target ORF information:

RefSeq Version	XM_021479006.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SPEG complex locus b (spegb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479006.1

ORF Insert Sequence:

ATGAGGAAGG CTGAGGTACA GATGACCCTG AAGAAGGGTG CTGATGAAGA AGCTGGACGA 
ATGGCTCCTC CAAGCCCCAG GATACCATCG AAACGGGCAA AAGTTAGTTC TGAGCAGGTC 
GTCCCAGATC CCTCCGCATC CGTCTACCCA ACTCCAACTC CTCCAGTGTT CATCCGGAAG 
ATGAGGAACG CTGCGGTGGG TACTGGCTGC GATATCCGTT TGAAAGTGGC CGTGGCTGGA 
GACCCACAGC CTACTTTGTA CTGGTACCAC AACGATGACC TGGTCAACAT GGACAACCAG 
GAATATGGAG GCCTGTGGAT CAGGGACTGT AAACCCAGTG ATGCCGGCCT TTATACCTGC 
ATCGCCACCA ACCATCTTGG AGAAGCCAGA AGCAGTGCAG TCCTAGCTGT ATTGGATTTG 
GGTGAAGAAA ACCGGACACT CTACCCGAAC ATTTGGGCGG TTCACTCATG CGAAGTTTTC 
CGCTCCTGTC GGAAACAAAG TTATGACTCT GAGACTACAG AAGACGAAGC CGCTGAGCCA 
CAAATACCCC TGGAGGCCAA AGAAGGAGCC AGAGGTTTTC AGGATGAGGC CCAACGAGTG 
CCTGCAGGTG AGAGAATGAT GGATGTCGCC CCAGATGCGA GCAATCGCTG GGCCGCCAGT 
GAAAGCACAG TGTTGGAGAG AGAAGCTCTA GCCCTGGGAT CGAGAGCCCC AGGGCCGCAG 
TCTGCCAGGA GCCCATCTGA CGATGTTTTG AGGGATTCTC CCCCTCAAGT TCTACCACCC 
CCTTCCCCCA AATTTGGCCG CTCTACTGCC TCTCCTTTGC TCAGCCGTTC TGGTTCGGGC 
CCTGCCACAC CCCTTGCCCC TCGCAAGAAA GTTGTCGTGC CAACTGAATA TCAAGACACA 
GTACCAGGAG AGTTTGAGGA GAAGATTAAG CAACCCAAAT CCTCTGGGAT GTCTCAGAGT 
AGTGCTCAAG ACTCTCGACC CCAAACCCCT GTCAGTGAGT ATTCCCGTAA GGAGCTTACA 
CTTCGGCCCT CACCAAAACT GACAAGAGCT AGCTCCAAAA TCTTTGAGAA GGTGCGAGTC 
TTCGAAGAGC GCAGAAGAAG CATTGATAAC CCTGAAGGAT CTATATCTGG ACGTTCTTGG 
GCTGGATTTA ACAGAGCTTC ATCTATCGAC TCTGATGAAG GAGGGAGCAG GTTGGGCATC 
TCCAGGGAGA GCTCCAAAGA AGACCTTCGT GAGGCTTTGA AGGCAGATGC AGCCCAACGA 
AGGACCATGT TTAAACAGCG GGCTGCTTCC CTGGAGGACA GACCTCGTTA CACCCAGAAG 
GTGCAGGACA TTGAAAACAA GTTCACAGAG GAGCTCCAGC GCATCAAAAA GCTTGTCGGC 
AAACCACACA TGAAGAAATC CTTCTCAACA GAGCAGCTCT CTACTTGTCA CAGAAGCAGG 
CAGCCTGTAA GAAAGTTAGA GCCCATCCCA CCTCAGGTCC TTCAAAAGCT GCAGGATAGA 
GAACGTGCTC AGCTAGCTCA GCTAGAGCAA GAGATGAGAG AGCGAGACCA GGCCAAAGAA 
CGATCACCTC CGAAATCACC GTCTCGCAGA AGGAAAGACC ATCTGGCACA ACAACCACCA 
GAAAGAGCTG AGACCAAGGT GGTAAGCACA ACTGGACTTG AACCATCCCC TCCAGAAGCC 
ATGTCACTCT CTGATTTACC AGGTCAAAGA TCTCCAAGAT TTCGGGGCCC AAGCCCAAGC 
AGAGATAGTA CACGAAGGTC TCCAACCGTA GAGATTAGTG CCATGGCTGA GGAGCGCCCT 
CGAGGAAGAG CTGAATCTCC TTCAAGAAAT GTTTTGGAGA TGACGTTACG CAAAGTGGAG 
CCAAGACCTG CAAGTCCACT GGTAAAGAGG GTGGTTCGTG TTCAAGAAGT TCCACAAGCA 
AACGATCAGC CTATGCATAG AAAGACGCCA ATAGAGGTCT CGCTAAGAAA ACTGGAAAGA 
AGGCCTGAAA GTCCCCTGGT GCAAGGAGAA ACCGTAGCCA TTCAAGACTT CCCTGTTCAA 
GCTCCCCCCA AACCTCCAAG AGTTTCCTCA TCTTCCTCGT CTGAGGAGAA GATGGAGCTG 
GAAGTGACTC CTCCACCACC CCCACTAAAA CTCACTATAC CAAAAATTAT TGTTGAAGAA 
GAGCCCATGG AGACAGACAC ACCTGTTGAG AAGACTGAAA AGGCTGTGAA AGTTGGCACT 
GGTAAAGATG AAAAACATCA AAGATCCAGA GGAAGAGGAC GCAGACAAAG ACCAATGTCC 
CCTGAATTAG AATCTTCGGA CGACTCCTAT GTGTCTGCAG GAGAGGATCC ACTAGAAGCT 
CCTGTGTTCG AGTTTCCACT TCAGGACACT GTGGCCTCCA CTGGAACGGA GGTGCTATTA 
AAATGCATCA TCAGTGGCAC CCCTCTCCCT GAAGTTATAT GGAAGAAAGA CAACACTGAG 
GTCAAGAACA GTCCCACTCA TGTTGTAAAG ATAGAGGGGG AACGCCACTC CTTGCTCATC 
AAATGGACAA AGCCAAGTGA TGCAGGAACC TACACAGTTA CCGCTGTCAA TGAGGTCGGT 
GAGGTGTCCA GCAGTGCTAC ACTTTTCATC AAACCAGAGC CCAGTCAGGA CCATCGTGGT 
AACCTTGGTG TCCCTATGGA TGTCAGCAGT CCCATCACAT CTGATGAGGA GTATCTCAGT 
CCACTGGAAG AGAGCATGGA CTTCTCCTCC TACAGGGGAC CGGAGCCAAG AAAGATCGTG 
GACACTCGAT TTAAGGAGCC TCCAGCCTTT CTGGCTGTTA TTGGAGACCA AAGCGTAATA 
GAGGGTCAGG AGGTGACCAT GTCTGTTCTA GTGACCGGAC AACCTAAACC TATGCTTTAC 
TGGCTGAGAG ACAGAGTGAC CATTAAAACC GGAACACGGC ATATCGTCCA AGAGACAGAG 
GAGGGCAACT TTGAAATGAT AATTAAATCA GCACAGAGGT CAGACACCGG TGTTTACACC 
TGCAAGATCA TCAATGAATA TGGAACTAAG CAATGTGAAG GCAAACTGGA GGTGAAAGCT 
CGTCCAGTAG AGCCAGGTCT GGCCATCATT CGGCCAGTGA GGGACGTGAT GGTGAAGGCA 
GGGGAGACCG CTCTGTTTGA GTGTCATGTG ATCGGCCCGC AGGACACAGA TGTTGACTGG 
CTGTCAGATG GGAAACTCAT CCAGCCTGCT TTGTTGAACT GCAAGATGCA TTTTGATGGG 
AAAAGGTGCA GGCTGCTTCT AAACTCTGTA CATGAGGATG ACAGTGGAAC GTACACCTGC 
AAACTGAGCA CAGCTAAAGA GGAACTGACC TCCAGTGCCA AGCTAAAAGT CATAGCATCC 
ATAGAGCCCC TTTTCACTCG TAAGCTGGAT GTGCTGGAAG TTATCGAGGG TCGTAATGCA 
CGATTTGACT GCAAGGTCAG CGGGACGCCG CCGCCTCAAG TCATATGGAG CCACTTCGAT 
CGTCCGCTCG AGGAAAACGA AGATATCAGG ATCTTAAAAG AAGGAGGACG GCACTCATTA 
ATCATCTCTC ATGTTAGTAA TGAAGATGAG GGCCTCTACA CGGTTGCAGC GCGAAACTCA 
CACGGGGAAG ACGAATGTGC AGCGGAGCTC TATGTGCAGG AGCCCAGACC AGCAATATCT 
TCTCAAATAG CGAAGCTGGA AAAAATGCCT TCCATTCCCG AAGAACCCGA AGTTCCTGAA 
AATGAGATTG AGCGTTTCAC CATGCCTGAC TTCGTAAAGC CGCTTTATGA TATGGATGTA 
GTGGAGGGCA GAGAGGCTGT GCTCAGGTGC AAAGTGGCCG GCTTGCCGTA CCCCACCATA 
ACATGGTTTC ACAATGGCAA GAGAATTGAC AGCACGGAGG ACAGAAAGAT GACACAGTTC 
AGAGATGTTC ACAGCCTGGT GGTCCGCTGT GTTTGTCATG CTCACGGAGG GGTTTACAAG 
TGTGTCATAT CCAATAAAGT GGGCAAGGCC ACCTGCTATT CTCATCTTTA CGTGACAGAC 
ATCCTTCCTG ACCCTCCAGA TGGTGCCCCA GTCGTTGAGT CCATCACTGG CAAGACTATC 
ACGCTAAGCT GGAAGAAACC CAAAAGACTG GATCCGTCCA TTGACCCCAG CTCCCTGATG 
TATGCCATTC AGCAACAGGC CCTGGGGTCG ATCCAGTGGA CCATCATCGC TTCTTGCCTG 
AAACAGACCA CCTATACCAT CAGCAATCTG TCTAAAGGTG TCCGCTACGC CTTCAGGGTG 
CTATCAATCA CCAGCAAGGC CTTTAGCAAG CCGTCGCCTG CCACCGAGCC AATGCAGCTG 
CTAGATAGAG GTCCGTACGT CCAGGAGGCT CCGGTAATCA TTGACAAGCC AGATATCGTG 
TATATGGTGG AGAAGCAGCC CTTGAGCATC ACAGTCACTC TCAACCACGT CAACGCATCG 
GTCACATGGA AGAGAGGAGG AGTGACATTA TCTAACCGAC CGGGTCTTTT TGAGTTGAGC 
ATGCCTGATG ATGACCAGCA CACACTGAAG CTGTGTAAGG TGAAGAGCAG TGATGTTGGC 
CAGCTCACCT GCATTGCCAA CAACAAGTAC GGCAGCGACT CCTGTGTCCT CACCCTGGAG 
ATGGCAGTGG CACCAACTTT TGAGACAATT ATGGAAGACC TGGATGTGAA TGTAGGCGAG 
ACTCCTCGAT TTGCAGTGGT GGTTGAAGGA AAGCCCGTCC CAGACATCCT CTGGTACAAG 
GGAGACACCC TTCTGTCCGA AAGCAGCCAT TTCACATTTG TGTATGATGA CAATGAATGT 
TCTCTAGTGG TGCTCAACAC TCAAGCAGAC GACTCAGGAG TGTACACTTG TACTGCGAAG 
AATCTGGCCG GTTCTGTGTC CTGCAAGGCT GAGCTGACCG TCCACACCAC CAAACAGTAC 
AAAGACGACC CGATGGAGGA TGAGGCGTCC ATCCTCAGAA AGATGCGGAG GCTCACTGAT 
TACTATGATG TGCACAAGGA AATTGGGAGA GGGGCTTTCT CATATGTGAA GCGGGTGATA 
CAGAAGGCAG GAAAGCTGGA ATACGCAGCC AAGTTTATTT CTGCTCGGGC TAAACGAAAG 
GCATCCGCTC TGAGAGAACT GAACATCCTC TCTCATCTGG ACCATGAGAG GATCCTCTAT 
TTTCATGATG CCTTTGAGAA GAAGAATGCT GTCATCATCA TTACAGAGCT ATGCCATGAG 
GAGCTTCTCG ATAGGCTTAC AAAGAAATCA ACCATCTTGG AGTCCGAGAT CCGATCCAGC 
GTGAGGCAGC TGTTGGAGGG TATTAACTAT CTCCATCAGC TTGACATACT TCATCTAGAC 
ATCAAGCCTG ATAATATCCT CATGGCAGAC CACAGTAGCG ATCAGATCCG CATATGTGAT 
TTTGGAAATG CTGTAAAGTT CATGCCTGAT GAGGCACAGT ACTGCAAATA TGGCACTCCA 
GAATTTGTCG CCCCAGAGAT TGTTAACCAG ACACCCGTCT CCAAGGCAAC GGACATCTGG 
CCCATTGGTG TTCTGACATA CTTATGTCTT ACTGGAGTTT CTCCTTTTGC TGGGGAAAAC 
GACCGCAGCT CTGTTCTCAA CATCAGGAAT TATAATGTGG CTTTTGAAGA AAGCATGTTT 
ACAGACCTCT GCCATGAAGC CAAGGGATTC GTCATCAAGC TGCTGGTGGC TGATAGATTG 
AGGCCTGATG CTAATGAATG TCTTCGCCAC CCCTGGTTCA AGACCCTCAA CAAGGGTAAA 
AGCATCAGTA CAGAGTCCCT TAAAAAGTTT TTGTCCAGAA GAAAATGGCA GCGATCGCTT 
ATCAGCTACA AATCTAAAAT GGTGATGAGG TCTGTACCTG AGCTACTGGA TGACTCCTCC 
AGCCATATTT CCATTGCTGT TCCGCGACAT CTGAAAAAAG GCTCACCACC ACTTTCATCA 
TCTTCAGATT CAGATGAAGA CATCGATGAG TTGCCTTTTA TTCCCATGCC ACTCAACGTG 
GAATTCTCAG GATCTAGAAT GTCACTGACC GAAATTGTAG GTGATGATGA CATGACAGGT 
AAATTGAATG GAAATGCTGA GAGGCCTGCT TCACTCCATC CGAAGTCCGA GCCAATGGAA 
GTGGAGATGT CAGAGAGAGA AGGTGGAGAT CCATCTGCTA GGGGTAGAGC CAAGAAGAGG 
ACTACAACAG AAGATGAAAA AGGGTCGTCT GATGAGGAAC CATCACAACT GGCCAAAAGA 
GCAGAACTGG CAAAGAGACC AATGCGCAGA GGTTCAAGCA TGGAGTCAGA TGAGCCAGAG 
GGAGGACGTC GCAGAGGAGA GTTACGCAGA GGGAGTTCGG CTGACAGTGC CTTACAGCTC 
CATATCAAAC CAGAGGAGGG CACAGAAGAA AGTCCTACTG ATGGAAGTAG AATTCTACAA 
AAGTCTGTAT CTATGGAGCT TCCAAGGAGA AGCCCAAGTC CAGGTGCTGC AAAGCTTAGC 
CAGGAAGACT ATGCTCTGAA ACTGGAACTG ATGAGGCAGC GACTACTCAG GGGTGGCTCT 
GCTGACAACA AGATGAGTGG ATTACGAGGC CCATTGCTGG AGACACTAGG TATGGGAGAT 
GAGAAGCGGT TTGTGCGGGG ATCTCGTCTA GGTAACCCTC CACTTGTTAG AGCGGCATCT 
AGTGAGTCAC CTAGAGATGA CATCCCAAAG ACAAAGATTC TCCGCAAGAG TGCTTCCTTT 
AGCCAGGGGG ATTCAGAGCC CATGCCCCTG CACAGAAGAA TCGGAGCACC TCTAGAGATT 
CCTCTAGCAC AAATAGAGGA GAGACGCCTA CAGGAAGCAA TATCCATGTC TGCTCTCACA 
GAGCCTGTCA AAGATTCTCG CCCAATCACT CCAAAACTTC AAATGCCTGA AGAAGAAAGA 
CATGAAAAGA AGAAAACTGA AGAAAAAGAA TCTGTCTACA AGGACAATAA ACCTGAAGAG 
GTTATTGCTG TGTCAAAGAC TGAGGAAGAA GGAAGACGTG TTGATCAGAA ATCTTGGGAT 
TCTGATGCAC AGAAAAAGGA CCAGAGCAAG TCAGAGGAAA CCAGGGAAGA GTCTGAAAAA 
GTGGCTGAAA TTGAAAAAAA GACCTCTATA GTGGACGAAA CAATTGAGGA AGAGGAAACA 
AGAAAAGATG AGGAGCCAGA GAAAGAAACT GAGCCTCCTA CTCCAAAGAT TGAGGTTAAG 
GAATATGAGG GTGATGTAAA GGTATTACCA AAGTCTCCAG AGTTTATCAT TTCCACAAAC 
TCAATCATGG TGACCCCAAC AGCTGCCCCA GCATCCAGTC CCCAGAGTGT ATCAACCTAT 
GTTACCCCAT CTCCCCCTGC ACGCACAGTG CTCCCAGATG GTAGGATGTC CGCATATGCC 
AGTATTATGC AGACTATCAT GGTACCATCT GTCCCAGCAA ATACCCAATC TGACACTGTT 
CCTATTCCTA CACCAACTTC CACTCCACCA TCAGTTCCAG TCTATGCACC CATTTCCAGA 
CACATCTCAG AACCAACCCT TTCATCAACT TCTTCATCCA AATCAGATCT TCACCAAACA 
ACTGAGCATC CTGCAGTCTT TTCTCGGGTT GCCTCCACTG AACACCCTCC CAAAGAGCCA 
AGTCCTCCTA AGACACCCAC TCGCGCCTCC CCTAAGCGAG AGCCATCTCC TGGAAGCAAC 
ATCCAGGACC TTGCATCCGA GGAGGTTTTT GAAGCTCGCT TCAAGAAACG TGAGTCTTCT 
CTAACTCGTA GTCTCAAACG TTTCTCATTT CAGAAGACTG AAGACAAGCC CTCCACTGTT 
TCTCCTGAAA CTGCTGAAGA AATGTATCGC CCTGGACCAA CTGGTGCTCC TTTAGAGTTT 
GTGCCAAGGA GGTTGGAGGA GAAGTCCAAA TCAGTACAAG ATCTTCGAGA GGCAGAGAAG 
GACCCTGGGT TCATGAGGCG ACTCTCCATG CGCTTGAAAA GGACCCCATC AGTGGAAAAG 
CAGGAGGAGA AGCTCAAAGA GGAGGAGGCT TCAGCACCCA GGCGTCGTCT TTCTTGGGCA 
CTGGGACGGA GGGGATCCCA AGAGAAGAAG GAGATTGAGA TGATGAGGTT GGATGGGGGT 
CCTGAGCCTC CCCCAGAGCC TGAGTCTAAA GCACCTTCTG AGTCACCTAT TCTTGCCATG 
CGCAAGAAGA TTGGCAGCAC CATGGCAAGC TTATCTACAA GAATTAGGAG CCATTCTGAG 
GACAGGAAAG ATGACAGTGA AGGCAAGATG GAGACCAAGA GGACTCCTAT ATTCAACAAA 
CTAAGGCGTT CCACTTCTGA GGGGGGCAGT CTGAAGAGAA TGGGTATCCC ACAAAACCAA 
CTGGCTGCTC AGTCAGGGCA AGGGACCTCA TCAGAATCTC TTGATTCTAT GTCAAGCATC 
CAGTCAGAAT CAGCTATTAA AAATGCAGAA CTTGACCGCA GATCACGGTG GGACCGATGG 
GGCTTGACTC GAGGGAAAAA GGACAAGACA GTTTCCCAAC CAGACATCCC AGCTTCCATT 
GCTAGAGAGA ATGGTTCTCT ACGGTCTCGC CAATATACAC GTCTAACATC TGACTTCCCT 
CCGGTGTTCC ATATCAAACT GAGGGACCAT GTACTCTTGG AGGGAGATCC TGTCACTCTC 
AGCTGCCTCC CTGCTGGCAG TCCTCACCCA CACATCTCAT GGATGAAAGA CAAGAAGCCA 
CTTGAGATTG ACCCCAGGAT GAATATGATA TCTTGCCCCG ATGGGAGACA ACTGCTGATG 
ATTATGAAGA CCACCAAGAA GGATGCAGGC CTCTATGAAT GTGTGGCTGC AAACCCACTG 
GCCACAGTCA CCAGCTCATG CGTTGTGTCC CTCGCTCGTC TGCCTAATAG TCCTGGGACA 
CCCGAGGTTC CTCAGAAGTA TAAAAACACT GCTCTAGTGG TATGGAGACC CTCAGACACT 
ACGGCACCCT GCACCTACTC TCTGGAGAGG AAGACAGAGG GTGAGAATAA CTGGCTGATA 
GTCGCCACAG GTGTTGCTGA CTGTTACTAT AACGTGTTGG ATCTTCCGGC CGGCGCTTCA 
TACAGGTTCA GAGTGGCCTG CGTCAATAAG GCTGGTCAGG GACCTTACAG CAGCTTGTCT 
GAAGTAGTGG TCCTGGACTC AACAGAGCCC CAGAAGTCCA GTGGCACAGT TGTAGTCAAA 
ACGCTTTCAT CAGCTCCCGC TCCTGCAGTG GTAACGTCCA CCATGACCCT TCCTGCAATG 
AAGCCTGCTG CAATAAAACC AGCACCATCA ACAAATTCAG CGCCCTCTAC AGTCCAACCG 
GTAAAGCCTG CGCCTCCTCC TGCATCATCA ACACCTACCT ATCGTCCCGT GCCTGACATT 
CGAACTCCAT CGCCTGGCAC TCCAACCCCT GCCAAAGCCA AAAGCACCCT CAACATTACT 
ATGGCCAAAC CCTCCCCAGC ATCCCCTGCA GCTCAACCAC AACCACAACC TCCACCCAAA 
CCCTCGCCTC CGGTCATTCT GCCCAAACCT CAGACCCCAG TCAGTGTGGT TTCACCTCCA 
TCCCAAACTC CTCCAGTGTC ACCTCCACCT CTAGTGTCTC CTCCTCCCAT TATTGGTAAA 
CCAATCTCCT CAGTGCCCAT GTATGTCCCT GCTACAACCA CCTCGACTCC AGTGACTCCG 
GTCACTCCTG TCACCCCAGT CACTCCAGTC ACCCCAAATA CGCCCTCTCC TGCCCTGTCG 
CCTCCAGTGG TGTTGGTTAC AAGTCTGAGT CCTGTAGGGG AGGGAACTGG GACACCCAAT 
CGAATGACCC CTAGTGGAAG AGCCACGCCT TCTGGACGCA TCACTCCATC AGGCCGAAGG 
ACCCCTATTG GAAAGCCTGG AGACTCAACG CTGAGGCAAG GAGTCCCTCA GAAACCTTAC 
ACCTTCATGG ATGAGAAAGC AAGGGGTCGC TTTGGTGTGA TCAGAGAGTG TCGAGAAAAT 
GCCACTGGTA ACCTGTACAT GGCTAAGATT GTGCCATATG AGCCAGAGAG CAAGCAGACT 
GTATTACAGG AATACGACAT CCTGAAGTCT CTCCATCATG AGAAGATCAT GGCTCTCCAC 
GAGGCCTACG TCACTCCACG CTACCTGGTG CTCATCTCTG AGTGCTGCTC TGGCAAAGAA 
CTACTCCACA GTCTCATTGA CAGGTTCCGA TACTCTGAGG ATGATGTGGT TGCATATATC 
GTACAGATCC TTCAGGGTCT GGACTACCTG CACAGCCGGA GGATCCTGCA TCTCGACATC 
AAACCAGAAA ACATCATCGT CACTTACATG AATGTGGTGA AGATTATCGA TTTTGGTAGT 
GCACAGACCT TCAATCCTCT GTTCCTGAAG CAGTTCTCCC CACCTATTGG AACACTGGAT 
TATATGTCTC CAGAGATGCT GAAAGGAGAT GTGGTGGGTC CACCAGCTGA CATCTGGAGC 
ATTGGCATAT TGACTTATAT CATGCTTAGC GGGAGGTTGC CGTTCACAGA AAATGACCCT 
GCCGAGACCG AGGCCAGAAT ACAGGCAGCC AAGTTTGACC TCAGTAAGCT TTACCAAAAC 
GTGTCCCAAA GTGCCTCTCT GTTCATCAAA AAGATCCTGT GCAGCTACCC TTGGGCCCGT 
CCGACCATCA AGGACTGCTT TACCAACTCG TGGCTGCAGG ACGCATATCT GATGCGCCTG 
CGAAGACAGA CTCTCACATT CACCACCACA CGCCTCAAAG AGTTCCTGGA GCAGCAGCAG 
CACATCAGAG CCCAAGAGGC AACAAAGCAC AAAGTCCTCC TGCGTTCCTA CCAGAGCAAC 
CCACAAACCC CATCCACCCC TCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spegb ( XM_021479007.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334682.1 Danio rerio SPEG complex locus b (spegb), transcript variant X2, mRNA.

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CloneID	ODa144103
Clone ID Related Accession (Same CDS sequence)	XM_021479007.1
Accession Version	XM_021479007.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	striated muscle preferentially expressed protein kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAAGG CTGAGGTACA GATGACCCTG AAGAAGGGTG CTGATGAAGA AGCTGGACGA 
ATGGCTCCTC CAAGCCCCAG GATACCATCG AAACGGGCAA AAGTTAGTTC TGAGCAGGTC 
GTCCCAGATC CCTCCGCATC CGTCTACCCA ACTCCAACTC CTCCAGTGTT CATCCGGAAG 
ATGAGGAACG CTGCGGTGGG TACTGGCTGC GATATCCGTT TGAAAGTGGC CGTGGCTGGA 
GACCCACAGC CTACTTTGTA CTGGTACCAC AACGATGACC TGGTCAACAT GGACAACCAG 
GAATATGGAG GCCTGTGGAT CAGGGACTGT AAACCCAGTG ATGCCGGCCT TTATACCTGC 
ATCGCCACCA ACCATCTTGG AGAAGCCAGA AGCAGTGCAG TCCTAGCTGT ATTGGATTTG 
GGTGAAGACT CTGAGACTAC AGAAGACGAA GCCGCTGAGC CACAAATACC CCTGGAGGCC 
AAAGAAGGAG CCAGAGGTTT TCAGGATGAG GCCCAACGAG TGCCTGCAGG TGAGAGAATG 
ATGGATGTCG CCCCAGATGC GAGCAATCGC TGGGCCGCCA GTGAAAGCAC AGTGTTGGAG 
AGAGAAGCTC TAGCCCTGGG ATCGAGAGCC CCAGGGCCGC AGTCTGCCAG GAGCCCATCT 
GACGATGTTT TGAGGGATTC TCCCCCTCAA GTTCTACCAC CCCCTTCCCC CAAATTTGGC 
CGCTCTACTG CCTCTCCTTT GCTCAGCCGT TCTGGTTCGG GCCCTGCCAC ACCCCTTGCC 
CCTCGCAAGA AAGTTGTCGT GCCAACTGAA TATCAAGACA CAGTACCAGG AGAGTTTGAG 
GAGAAGATTA AGCAACCCAA ATCCTCTGGG ATGTCTCAGA GTAGTGCTCA AGACTCTCGA 
CCCCAAACCC CTGTCAGTGA GTATTCCCGT AAGGAGCTTA CACTTCGGCC CTCACCAAAA 
CTGACAAGAG CTAGCTCCAA AATCTTTGAG AAGGTGCGAG TCTTCGAAGA GCGCAGAAGA 
AGCATTGATA ACCCTGAAGG ATCTATATCT GGACGTTCTT GGGCTGGATT TAACAGAGCT 
TCATCTATCG ACTCTGATGA AGGAGGGAGC AGGTTGGGCA TCTCCAGGGA GAGCTCCAAA 
GAAGACCTTC GTGAGGCTTT GAAGGCAGAT GCAGCCCAAC GAAGGACCAT GTTTAAACAG 
CGGGCTGCTT CCCTGGAGGA CAGACCTCGT TACACCCAGA AGGTGCAGGA CATTGAAAAC 
AAGTTCACAG AGGAGCTCCA GCGCATCAAA AAGCTTGTCG GCAAACCACA CATGAAGAAA 
TCCTTCTCAA CAGAGCAGCT CTCTACTTGT CACAGAAGCA GGCAGCCTGT AAGAAAGTTA 
GAGCCCATCC CACCTCAGGT CCTTCAAAAG CTGCAGGATA GAGAACGTGC TCAGCTAGCT 
CAGCTAGAGC AAGAGATGAG AGAGCGAGAC CAGGCCAAAG AACGATCACC TCCGAAATCA 
CCGTCTCGCA GAAGGAAAGA CCATCTGGCA CAACAACCAC CAGAAAGAGC TGAGACCAAG 
GTGGTAAGCA CAACTGGACT TGAACCATCC CCTCCAGAAG CCATGTCACT CTCTGATTTA 
CCAGGTCAAA GATCTCCAAG ATTTCGGGGC CCAAGCCCAA GCAGAGATAG TACACGAAGG 
TCTCCAACCG TAGAGATTAG TGCCATGGCT GAGGAGCGCC CTCGAGGAAG AGCTGAATCT 
CCTTCAAGAA ATGTTTTGGA GATGACGTTA CGCAAAGTGG AGCCAAGACC TGCAAGTCCA 
CTGGTAAAGA GGGTGGTTCG TGTTCAAGAA GTTCCACAAG CAAACGATCA GCCTATGCAT 
AGAAAGACGC CAATAGAGGT CTCGCTAAGA AAACTGGAAA GAAGGCCTGA AAGTCCCCTG 
GTGCAAGGAG AAACCGTAGC CATTCAAGAC TTCCCTGTTC AAGCTCCCCC CAAACCTCCA 
AGAGTTTCCT CATCTTCCTC GTCTGAGGAG AAGATGGAGC TGGAAGTGAC TCCTCCACCA 
CCCCCACTAA AACTCACTAT ACCAAAAATT ATTGTTGAAG AAGAGCCCAT GGAGACAGAC 
ACACCTGTTG AGAAGACTGA AAAGGCTGTG AAAGTTGGCA CTGGTAAAGA TGAAAAACAT 
CAAAGATCCA GAGGAAGAGG ACGCAGACAA AGACCAATGT CCCCTGAATT AGAATCTTCG 
GACGACTCCT ATGTGTCTGC AGGAGAGGAT CCACTAGAAG CTCCTGTGTT CGAGTTTCCA 
CTTCAGGACA CTGTGGCCTC CACTGGAACG GAGGTGCTAT TAAAATGCAT CATCAGTGGC 
ACCCCTCTCC CTGAAGTTAT ATGGAAGAAA GACAACACTG AGGTCAAGAA CAGTCCCACT 
CATGTTGTAA AGATAGAGGG GGAACGCCAC TCCTTGCTCA TCAAATGGAC AAAGCCAAGT 
GATGCAGGAA CCTACACAGT TACCGCTGTC AATGAGGTCG GTGAGGTGTC CAGCAGTGCT 
ACACTTTTCA TCAAACCAGA GCCCAGTCAG GACCATCGTG GTAACCTTGG TGTCCCTATG 
GATGTCAGCA GTCCCATCAC ATCTGATGAG GAGTATCTCA GTCCACTGGA AGAGAGCATG 
GACTTCTCCT CCTACAGGGG ACCGGAGCCA AGAAAGATCG TGGACACTCG ATTTAAGGAG 
CCTCCAGCCT TTCTGGCTGT TATTGGAGAC CAAAGCGTAA TAGAGGGTCA GGAGGTGACC 
ATGTCTGTTC TAGTGACCGG ACAACCTAAA CCTATGCTTT ACTGGCTGAG AGACAGAGTG 
ACCATTAAAA CCGGAACACG GCATATCGTC CAAGAGACAG AGGAGGGCAA CTTTGAAATG 
ATAATTAAAT CAGCACAGAG GTCAGACACC GGTGTTTACA CCTGCAAGAT CATCAATGAA 
TATGGAACTA AGCAATGTGA AGGCAAACTG GAGGTGAAAG CTCGTCCAGT AGAGCCAGGT 
CTGGCCATCA TTCGGCCAGT GAGGGACGTG ATGGTGAAGG CAGGGGAGAC CGCTCTGTTT 
GAGTGTCATG TGATCGGCCC GCAGGACACA GATGTTGACT GGCTGTCAGA TGGGAAACTC 
ATCCAGCCTG CTTTGTTGAA CTGCAAGATG CATTTTGATG GGAAAAGGTG CAGGCTGCTT 
CTAAACTCTG TACATGAGGA TGACAGTGGA ACGTACACCT GCAAACTGAG CACAGCTAAA 
GAGGAACTGA CCTCCAGTGC CAAGCTAAAA GTCATAGCAT CCATAGAGCC CCTTTTCACT 
CGTAAGCTGG ATGTGCTGGA AGTTATCGAG GGTCGTAATG CACGATTTGA CTGCAAGGTC 
AGCGGGACGC CGCCGCCTCA AGTCATATGG AGCCACTTCG ATCGTCCGCT CGAGGAAAAC 
GAAGATATCA GGATCTTAAA AGAAGGAGGA CGGCACTCAT TAATCATCTC TCATGTTAGT 
AATGAAGATG AGGGCCTCTA CACGGTTGCA GCGCGAAACT CACACGGGGA AGACGAATGT 
GCAGCGGAGC TCTATGTGCA GGAGCCCAGA CCAGCAATAT CTTCTCAAAT AGCGAAGCTG 
GAAAAAATGC CTTCCATTCC CGAAGAACCC GAAGTTCCTG AAAATGAGAT TGAGCGTTTC 
ACCATGCCTG ACTTCGTAAA GCCGCTTTAT GATATGGATG TAGTGGAGGG CAGAGAGGCT 
GTGCTCAGGT GCAAAGTGGC CGGCTTGCCG TACCCCACCA TAACATGGTT TCACAATGGC 
AAGAGAATTG ACAGCACGGA GGACAGAAAG ATGACACAGT TCAGAGATGT TCACAGCCTG 
GTGGTCCGCT GTGTTTGTCA TGCTCACGGA GGGGTTTACA AGTGTGTCAT ATCCAATAAA 
GTGGGCAAGG CCACCTGCTA TTCTCATCTT TACGTGACAG ACATCCTTCC TGACCCTCCA 
GATGGTGCCC CAGTCGTTGA GTCCATCACT GGCAAGACTA TCACGCTAAG CTGGAAGAAA 
CCCAAAAGAC TGGATCCGTC CATTGACCCC AGCTCCCTGA TGTATGCCAT TCAGCAACAG 
GCCCTGGGGT CGATCCAGTG GACCATCATC GCTTCTTGCC TGAAACAGAC CACCTATACC 
ATCAGCAATC TGTCTAAAGG TGTCCGCTAC GCCTTCAGGG TGCTATCAAT CACCAGCAAG 
GCCTTTAGCA AGCCGTCGCC TGCCACCGAG CCAATGCAGC TGCTAGATAG AGGTCCGTAC 
GTCCAGGAGG CTCCGGTAAT CATTGACAAG CCAGATATCG TGTATATGGT GGAGAAGCAG 
CCCTTGAGCA TCACAGTCAC TCTCAACCAC GTCAACGCAT CGGTCACATG GAAGAGAGGA 
GGAGTGACAT TATCTAACCG ACCGGGTCTT TTTGAGTTGA GCATGCCTGA TGATGACCAG 
CACACACTGA AGCTGTGTAA GGTGAAGAGC AGTGATGTTG GCCAGCTCAC CTGCATTGCC 
AACAACAAGT ACGGCAGCGA CTCCTGTGTC CTCACCCTGG AGATGGCAGT GGCACCAACT 
TTTGAGACAA TTATGGAAGA CCTGGATGTG AATGTAGGCG AGACTCCTCG ATTTGCAGTG 
GTGGTTGAAG GAAAGCCCGT CCCAGACATC CTCTGGTACA AGGGAGACAC CCTTCTGTCC 
GAAAGCAGCC ATTTCACATT TGTGTATGAT GACAATGAAT GTTCTCTAGT GGTGCTCAAC 
ACTCAAGCAG ACGACTCAGG AGTGTACACT TGTACTGCGA AGAATCTGGC CGGTTCTGTG 
TCCTGCAAGG CTGAGCTGAC CGTCCACACC ACCAAACAGT ACAAAGACGA CCCGATGGAG 
GATGAGGCGT CCATCCTCAG AAAGATGCGG AGGCTCACTG ATTACTATGA TGTGCACAAG 
GAAATTGGGA GAGGGGCTTT CTCATATGTG AAGCGGGTGA TACAGAAGGC AGGAAAGCTG 
GAATACGCAG CCAAGTTTAT TTCTGCTCGG GCTAAACGAA AGGCATCCGC TCTGAGAGAA 
CTGAACATCC TCTCTCATCT GGACCATGAG AGGATCCTCT ATTTTCATGA TGCCTTTGAG 
AAGAAGAATG CTGTCATCAT CATTACAGAG CTATGCCATG AGGAGCTTCT CGATAGGCTT 
ACAAAGAAAT CAACCATCTT GGAGTCCGAG ATCCGATCCA GCGTGAGGCA GCTGTTGGAG 
GGTATTAACT ATCTCCATCA GCTTGACATA CTTCATCTAG ACATCAAGCC TGATAATATC 
CTCATGGCAG ACCACAGTAG CGATCAGATC CGCATATGTG ATTTTGGAAA TGCTGTAAAG 
TTCATGCCTG ATGAGGCACA GTACTGCAAA TATGGCACTC CAGAATTTGT CGCCCCAGAG 
ATTGTTAACC AGACACCCGT CTCCAAGGCA ACGGACATCT GGCCCATTGG TGTTCTGACA 
TACTTATGTC TTACTGGAGT TTCTCCTTTT GCTGGGGAAA ACGACCGCAG CTCTGTTCTC 
AACATCAGGA ATTATAATGT GGCTTTTGAA GAAAGCATGT TTACAGACCT CTGCCATGAA 
GCCAAGGGAT TCGTCATCAA GCTGCTGGTG GCTGATAGAT TGAGGCCTGA TGCTAATGAA 
TGTCTTCGCC ACCCCTGGTT CAAGACCCTC AACAAGGGTA AAAGCATCAG TACAGAGTCC 
CTTAAAAAGT TTTTGTCCAG AAGAAAATGG CAGCGATCGC TTATCAGCTA CAAATCTAAA 
ATGGTGATGA GGTCTGTACC TGAGCTACTG GATGACTCCT CCAGCCATAT TTCCATTGCT 
GTTCCGCGAC ATCTGAAAAA AGGCTCACCA CCACTTTCAT CATCTTCAGA TTCAGATGAA 
GACATCGATG AGTTGCCTTT TATTCCCATG CCACTCAACG TGGAATTCTC AGGATCTAGA 
ATGTCACTGA CCGAAATTGT AGGTGATGAT GACATGACAG GTAAATTGAA TGGAAATGCT 
GAGAGGCCTG CTTCACTCCA TCCGAAGTCC GAGCCAATGG AAGTGGAGAT GTCAGAGAGA 
GAAGGTGGAG ATCCATCTGC TAGGGGTAGA GCCAAGAAGA GGACTACAAC AGAAGATGAA 
AAAGGGTCGT CTGATGAGGA ACCATCACAA CTGGCCAAAA GAGCAGAACT GGCAAAGAGA 
CCAATGCGCA GAGGTTCAAG CATGGAGTCA GATGAGCCAG AGGGAGGACG TCGCAGAGGA 
GAGTTACGCA GAGGGAGTTC GGCTGACAGT GCCTTACAGC TCCATATCAA ACCAGAGGAG 
GGCACAGAAG AAAGTCCTAC TGATGGAAGT AGAATTCTAC AAAAGTCTGT ATCTATGGAG 
CTTCCAAGGA GAAGCCCAAG TCCAGGTGCT GCAAAGCTTA GCCAGGAAGA CTATGCTCTG 
AAACTGGAAC TGATGAGGCA GCGACTACTC AGGGGTGGCT CTGCTGACAA CAAGATGAGT 
GGATTACGAG GCCCATTGCT GGAGACACTA GGTATGGGAG ATGAGAAGCG GTTTGTGCGG 
GGATCTCGTC TAGGTAACCC TCCACTTGTT AGAGCGGCAT CTAGTGAGTC ACCTAGAGAT 
GACATCCCAA AGACAAAGAT TCTCCGCAAG AGTGCTTCCT TTAGCCAGGG GGATTCAGAG 
CCCATGCCCC TGCACAGAAG AATCGGAGCA CCTCTAGAGA TTCCTCTAGC ACAAATAGAG 
GAGAGACGCC TACAGGAAGC AATATCCATG TCTGCTCTCA CAGAGCCTGT CAAAGATTCT 
CGCCCAATCA CTCCAAAACT TCAAATGCCT GAAGAAGAAA GACATGAAAA GAAGAAAACT 
GAAGAAAAAG AATCTGTCTA CAAGGACAAT AAACCTGAAG AGGTTATTGC TGTGTCAAAG 
ACTGAGGAAG AAGGAAGACG TGTTGATCAG AAATCTTGGG ATTCTGATGC ACAGAAAAAG 
GACCAGAGCA AGTCAGAGGA AACCAGGGAA GAGTCTGAAA AAGTGGCTGA AATTGAAAAA 
AAGACCTCTA TAGTGGACGA AACAATTGAG GAAGAGGAAA CAAGAAAAGA TGAGGAGCCA 
GAGAAAGAAA CTGAGCCTCC TACTCCAAAG ATTGAGGTTA AGGAATATGA GGGTGATGTA 
AAGGTATTAC CAAAGTCTCC AGAGTTTATC ATTTCCACAA ACTCAATCAT GGTGACCCCA 
ACAGCTGCCC CAGCATCCAG TCCCCAGAGT GTATCAACCT ATGTTACCCC ATCTCCCCCT 
GCACGCACAG TGCTCCCAGA TGGTAGGATG TCCGCATATG CCAGTATTAT GCAGACTATC 
ATGGTACCAT CTGTCCCAGC AAATACCCAA TCTGACACTG TTCCTATTCC TACACCAACT 
TCCACTCCAC CATCAGTTCC AGTCTATGCA CCCATTTCCA GACACATCTC AGAACCAACC 
CTTTCATCAA CTTCTTCATC CAAATCAGAT CTTCACCAAA CAACTGAGCA TCCTGCAGTC 
TTTTCTCGGG TTGCCTCCAC TGAACACCCT CCCAAAGAGC CAAGTCCTCC TAAGACACCC 
ACTCGCGCCT CCCCTAAGCG AGAGCCATCT CCTGGAAGCA ACATCCAGGA CCTTGCATCC 
GAGGAGGTTT TTGAAGCTCG CTTCAAGAAA CGTGAGTCTT CTCTAACTCG TAGTCTCAAA 
CGTTTCTCAT TTCAGAAGAC TGAAGACAAG CCCTCCACTG TTTCTCCTGA AACTGCTGAA 
GAAATGTATC GCCCTGGACC AACTGGTGCT CCTTTAGAGT TTGTGCCAAG GAGGTTGGAG 
GAGAAGTCCA AATCAGTACA AGATCTTCGA GAGGCAGAGA AGGACCCTGG GTTCATGAGG 
CGACTCTCCA TGCGCTTGAA AAGGACCCCA TCAGTGGAAA AGCAGGAGGA GAAGCTCAAA 
GAGGAGGAGG CTTCAGCACC CAGGCGTCGT CTTTCTTGGG CACTGGGACG GAGGGGATCC 
CAAGAGAAGA AGGAGATTGA GATGATGAGG TTGGATGGGG GTCCTGAGCC TCCCCCAGAG 
CCTGAGTCTA AAGCACCTTC TGAGTCACCT ATTCTTGCCA TGCGCAAGAA GATTGGCAGC 
ACCATGGCAA GCTTATCTAC AAGAATTAGG AGCCATTCTG AGGACAGGAA AGATGACAGT 
GAAGGCAAGA TGGAGACCAA GAGGACTCCT ATATTCAACA AACTAAGGCG TTCCACTTCT 
GAGGGGGGCA GTCTGAAGAG AATGGGTATC CCACAAAACC AACTGGCTGC TCAGTCAGGG 
CAAGGGACCT CATCAGAATC TCTTGATTCT ATGTCAAGCA TCCAGTCAGA ATCAGCTATT 
AAAAATGCAG AACTTGACCG CAGATCACGG TGGGACCGAT GGGGCTTGAC TCGAGGGAAA 
AAGGACAAGA CAGTTTCCCA ACCAGACATC CCAGCTTCCA TTGCTAGAGA GAATGGTTCT 
CTACGGTCTC GCCAATATAC ACGTCTAACA TCTGACTTCC CTCCGGTGTT CCATATCAAA 
CTGAGGGACC ATGTACTCTT GGAGGGAGAT CCTGTCACTC TCAGCTGCCT CCCTGCTGGC 
AGTCCTCACC CACACATCTC ATGGATGAAA GACAAGAAGC CACTTGAGAT TGACCCCAGG 
ATGAATATGA TATCTTGCCC CGATGGGAGA CAACTGCTGA TGATTATGAA GACCACCAAG 
AAGGATGCAG GCCTCTATGA ATGTGTGGCT GCAAACCCAC TGGCCACAGT CACCAGCTCA 
TGCGTTGTGT CCCTCGCTCG TCTGCCTAAT AGTCCTGGGA CACCCGAGGT TCCTCAGAAG 
TATAAAAACA CTGCTCTAGT GGTATGGAGA CCCTCAGACA CTACGGCACC CTGCACCTAC 
TCTCTGGAGA GGAAGACAGA GGGTGAGAAT AACTGGCTGA TAGTCGCCAC AGGTGTTGCT 
GACTGTTACT ATAACGTGTT GGATCTTCCG GCCGGCGCTT CATACAGGTT CAGAGTGGCC 
TGCGTCAATA AGGCTGGTCA GGGACCTTAC AGCAGCTTGT CTGAAGTAGT GGTCCTGGAC 
TCAACAGAGC CCCAGAAGTC CAGTGGCACA GTTGTAGTCA AAACGCTTTC ATCAGCTCCC 
GCTCCTGCAG TGGTAACGTC CACCATGACC CTTCCTGCAA TGAAGCCTGC TGCAATAAAA 
CCAGCACCAT CAACAAATTC AGCGCCCTCT ACAGTCCAAC CGGTAAAGCC TGCGCCTCCT 
CCTGCATCAT CAACACCTAC CTATCGTCCC GTGCCTGACA TTCGAACTCC ATCGCCTGGC 
ACTCCAACCC CTGCCAAAGC CAAAAGCACC CTCAACATTA CTATGGCCAA ACCCTCCCCA 
GCATCCCCTG CAGCTCAACC ACAACCACAA CCTCCACCCA AACCCTCGCC TCCGGTCATT 
CTGCCCAAAC CTCAGACCCC AGTCAGTGTG GTTTCACCTC CATCCCAAAC TCCTCCAGTG 
TCACCTCCAC CTCTAGTGTC TCCTCCTCCC ATTATTGGTA AACCAATCTC CTCAGTGCCC 
ATGTATGTCC CTGCTACAAC CACCTCGACT CCAGTGACTC CGGTCACTCC TGTCACCCCA 
GTCACTCCAG TCACCCCAAA TACGCCCTCT CCTGCCCTGT CGCCTCCAGT GGTGTTGGTT 
ACAAGTCTGA GTCCTGTAGG GGAGGGAACT GGGACACCCA ATCGAATGAC CCCTAGTGGA 
AGAGCCACGC CTTCTGGACG CATCACTCCA TCAGGCCGAA GGACCCCTAT TGGAAAGCCT 
GGAGACTCAA CGCTGAGGCA AGGAGTCCCT CAGAAACCTT ACACCTTCAT GGATGAGAAA 
GCAAGGGGTC GCTTTGGTGT GATCAGAGAG TGTCGAGAAA ATGCCACTGG TAACCTGTAC 
ATGGCTAAGA TTGTGCCATA TGAGCCAGAG AGCAAGCAGA CTGTATTACA GGAATACGAC 
ATCCTGAAGT CTCTCCATCA TGAGAAGATC ATGGCTCTCC ACGAGGCCTA CGTCACTCCA 
CGCTACCTGG TGCTCATCTC TGAGTGCTGC TCTGGCAAAG AACTACTCCA CAGTCTCATT 
GACAGGTTCC GATACTCTGA GGATGATGTG GTTGCATATA TCGTACAGAT CCTTCAGGGT 
CTGGACTACC TGCACAGCCG GAGGATCCTG CATCTCGACA TCAAACCAGA AAACATCATC 
GTCACTTACA TGAATGTGGT GAAGATTATC GATTTTGGTA GTGCACAGAC CTTCAATCCT 
CTGTTCCTGA AGCAGTTCTC CCCACCTATT GGAACACTGG ATTATATGTC TCCAGAGATG 
CTGAAAGGAG ATGTGGTGGG TCCACCAGCT GACATCTGGA GCATTGGCAT ATTGACTTAT 
ATCATGCTTA GCGGGAGGTT GCCGTTCACA GAAAATGACC CTGCCGAGAC CGAGGCCAGA 
ATACAGGCAG CCAAGTTTGA CCTCAGTAAG CTTTACCAAA ACGTGTCCCA AAGTGCCTCT 
CTGTTCATCA AAAAGATCCT GTGCAGCTAC CCTTGGGCCC GTCCGACCAT CAAGGACTGC 
TTTACCAACT CGTGGCTGCA GGACGCATAT CTGATGCGCC TGCGAAGACA GACTCTCACA 
TTCACCACCA CACGCCTCAA AGAGTTCCTG GAGCAGCAGC AGCACATCAG AGCCCAAGAG 
GCAACAAAGC ACAAAGTCCT CCTGCGTTCC TACCAGAGCA ACCCACAAAC CCCATCCACC 
CCTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334682.1
CDS	712..11460
Translation	MRKAEVQMTLKKGADEEAGRMAPPSPRIPSKRAKVSSEQVVPDPSASVYPTPTPPVFIRKMRNAAVGTGCDIRLKVAVAGDPQPTLYWYHNDDLVNMDNQEYGGLWIRDCKPSDAGLYTCIATNHLGEARSSAVLAVLDLGEDSETTEDEAAEPQIPLEAKEGARGFQDEAQRVPAGERMMDVAPDASNRWAASESTVLEREALALGSRAPGPQSARSPSDDVLRDSPPQVLPPPSPKFGRSTASPLLSRSGSGPATPLAPRKKVVVPTEYQDTVPGEFEEKIKQPKSSGMSQSSAQDSRPQTPVSEYSRKELTLRPSPKLTRASSKIFEKVRVFEERRRSIDNPEGSISGRSWAGFNRASSIDSDEGGSRLGISRESSKEDLREALKADAAQRRTMFKQRAASLEDRPRYTQKVQDIENKFTEELQRIKKLVGKPHMKKSFSTEQLSTCHRSRQPVRKLEPIPPQVLQKLQDRERAQLAQLEQEMRERDQAKERSPPKSPSRRRKDHLAQQPPERAETKVVSTTGLEPSPPEAMSLSDLPGQRSPRFRGPSPSRDSTRRSPTVEISAMAEERPRGRAESPSRNVLEMTLRKVEPRPASPLVKRVVRVQEVPQANDQPMHRKTPIEVSLRKLERRPESPLVQGETVAIQDFPVQAPPKPPRVSSSSSSEEKMELEVTPPPPPLKLTIPKIIVEEEPMETDTPVEKTEKAVKVGTGKDEKHQRSRGRGRRQRPMSPELESSDDSYVSAGEDPLEAPVFEFPLQDTVASTGTEVLLKCIISGTPLPEVIWKKDNTEVKNSPTHVVKIEGERHSLLIKWTKPSDAGTYTVTAVNEVGEVSSSATLFIKPEPSQDHRGNLGVPMDVSSPITSDEEYLSPLEESMDFSSYRGPEPRKIVDTRFKEPPAFLAVIGDQSVIEGQEVTMSVLVTGQPKPMLYWLRDRVTIKTGTRHIVQETEEGNFEMIIKSAQRSDTGVYTCKIINEYGTKQCEGKLEVKARPVEPGLAIIRPVRDVMVKAGETALFECHVIGPQDTDVDWLSDGKLIQPALLNCKMHFDGKRCRLLLNSVHEDDSGTYTCKLSTAKEELTSSAKLKVIASIEPLFTRKLDVLEVIEGRNARFDCKVSGTPPPQVIWSHFDRPLEENEDIRILKEGGRHSLIISHVSNEDEGLYTVAARNSHGEDECAAELYVQEPRPAISSQIAKLEKMPSIPEEPEVPENEIERFTMPDFVKPLYDMDVVEGREAVLRCKVAGLPYPTITWFHNGKRIDSTEDRKMTQFRDVHSLVVRCVCHAHGGVYKCVISNKVGKATCYSHLYVTDILPDPPDGAPVVESITGKTITLSWKKPKRLDPSIDPSSLMYAIQQQALGSIQWTIIASCLKQTTYTISNLSKGVRYAFRVLSITSKAFSKPSPATEPMQLLDRGPYVQEAPVIIDKPDIVYMVEKQPLSITVTLNHVNASVTWKRGGVTLSNRPGLFELSMPDDDQHTLKLCKVKSSDVGQLTCIANNKYGSDSCVLTLEMAVAPTFETIMEDLDVNVGETPRFAVVVEGKPVPDILWYKGDTLLSESSHFTFVYDDNECSLVVLNTQADDSGVYTCTAKNLAGSVSCKAELTVHTTKQYKDDPMEDEASILRKMRRLTDYYDVHKEIGRGAFSYVKRVIQKAGKLEYAAKFISARAKRKASALRELNILSHLDHERILYFHDAFEKKNAVIIITELCHEELLDRLTKKSTILESEIRSSVRQLLEGINYLHQLDILHLDIKPDNILMADHSSDQIRICDFGNAVKFMPDEAQYCKYGTPEFVAPEIVNQTPVSKATDIWPIGVLTYLCLTGVSPFAGENDRSSVLNIRNYNVAFEESMFTDLCHEAKGFVIKLLVADRLRPDANECLRHPWFKTLNKGKSISTESLKKFLSRRKWQRSLISYKSKMVMRSVPELLDDSSSHISIAVPRHLKKGSPPLSSSSDSDEDIDELPFIPMPLNVEFSGSRMSLTEIVGDDDMTGKLNGNAERPASLHPKSEPMEVEMSEREGGDPSARGRAKKRTTTEDEKGSSDEEPSQLAKRAELAKRPMRRGSSMESDEPEGGRRRGELRRGSSADSALQLHIKPEEGTEESPTDGSRILQKSVSMELPRRSPSPGAAKLSQEDYALKLELMRQRLLRGGSADNKMSGLRGPLLETLGMGDEKRFVRGSRLGNPPLVRAASSESPRDDIPKTKILRKSASFSQGDSEPMPLHRRIGAPLEIPLAQIEERRLQEAISMSALTEPVKDSRPITPKLQMPEEERHEKKKTEEKESVYKDNKPEEVIAVSKTEEEGRRVDQKSWDSDAQKKDQSKSEETREESEKVAEIEKKTSIVDETIEEEETRKDEEPEKETEPPTPKIEVKEYEGDVKVLPKSPEFIISTNSIMVTPTAAPASSPQSVSTYVTPSPPARTVLPDGRMSAYASIMQTIMVPSVPANTQSDTVPIPTPTSTPPSVPVYAPISRHISEPTLSSTSSSKSDLHQTTEHPAVFSRVASTEHPPKEPSPPKTPTRASPKREPSPGSNIQDLASEEVFEARFKKRESSLTRSLKRFSFQKTEDKPSTVSPETAEEMYRPGPTGAPLEFVPRRLEEKSKSVQDLREAEKDPGFMRRLSMRLKRTPSVEKQEEKLKEEEASAPRRRLSWALGRRGSQEKKEIEMMRLDGGPEPPPEPESKAPSESPILAMRKKIGSTMASLSTRIRSHSEDRKDDSEGKMETKRTPIFNKLRRSTSEGGSLKRMGIPQNQLAAQSGQGTSSESLDSMSSIQSESAIKNAELDRRSRWDRWGLTRGKKDKTVSQPDIPASIARENGSLRSRQYTRLTSDFPPVFHIKLRDHVLLEGDPVTLSCLPAGSPHPHISWMKDKKPLEIDPRMNMISCPDGRQLLMIMKTTKKDAGLYECVAANPLATVTSSCVVSLARLPNSPGTPEVPQKYKNTALVVWRPSDTTAPCTYSLERKTEGENNWLIVATGVADCYYNVLDLPAGASYRFRVACVNKAGQGPYSSLSEVVVLDSTEPQKSSGTVVVKTLSSAPAPAVVTSTMTLPAMKPAAIKPAPSTNSAPSTVQPVKPAPPPASSTPTYRPVPDIRTPSPGTPTPAKAKSTLNITMAKPSPASPAAQPQPQPPPKPSPPVILPKPQTPVSVVSPPSQTPPVSPPPLVSPPPIIGKPISSVPMYVPATTTSTPVTPVTPVTPVTPVTPNTPSPALSPPVVLVTSLSPVGEGTGTPNRMTPSGRATPSGRITPSGRRTPIGKPGDSTLRQGVPQKPYTFMDEKARGRFGVIRECRENATGNLYMAKIVPYEPESKQTVLQEYDILKSLHHEKIMALHEAYVTPRYLVLISECCSGKELLHSLIDRFRYSEDDVVAYIVQILQGLDYLHSRRILHLDIKPENIIVTYMNVVKIIDFGSAQTFNPLFLKQFSPPIGTLDYMSPEMLKGDVVGPPADIWSIGILTYIMLSGRLPFTENDPAETEARIQAAKFDLSKLYQNVSQSASLFIKKILCSYPWARPTIKDCFTNSWLQDAYLMRLRRQTLTFTTTRLKEFLEQQQHIRAQEATKHKVLLRSYQSNPQTPSTPQ

Target ORF information:

RefSeq Version	XM_021479007.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SPEG complex locus b (spegb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479007.1

ORF Insert Sequence:

ATGAGGAAGG CTGAGGTACA GATGACCCTG AAGAAGGGTG CTGATGAAGA AGCTGGACGA 
ATGGCTCCTC CAAGCCCCAG GATACCATCG AAACGGGCAA AAGTTAGTTC TGAGCAGGTC 
GTCCCAGATC CCTCCGCATC CGTCTACCCA ACTCCAACTC CTCCAGTGTT CATCCGGAAG 
ATGAGGAACG CTGCGGTGGG TACTGGCTGC GATATCCGTT TGAAAGTGGC CGTGGCTGGA 
GACCCACAGC CTACTTTGTA CTGGTACCAC AACGATGACC TGGTCAACAT GGACAACCAG 
GAATATGGAG GCCTGTGGAT CAGGGACTGT AAACCCAGTG ATGCCGGCCT TTATACCTGC 
ATCGCCACCA ACCATCTTGG AGAAGCCAGA AGCAGTGCAG TCCTAGCTGT ATTGGATTTG 
GGTGAAGACT CTGAGACTAC AGAAGACGAA GCCGCTGAGC CACAAATACC CCTGGAGGCC 
AAAGAAGGAG CCAGAGGTTT TCAGGATGAG GCCCAACGAG TGCCTGCAGG TGAGAGAATG 
ATGGATGTCG CCCCAGATGC GAGCAATCGC TGGGCCGCCA GTGAAAGCAC AGTGTTGGAG 
AGAGAAGCTC TAGCCCTGGG ATCGAGAGCC CCAGGGCCGC AGTCTGCCAG GAGCCCATCT 
GACGATGTTT TGAGGGATTC TCCCCCTCAA GTTCTACCAC CCCCTTCCCC CAAATTTGGC 
CGCTCTACTG CCTCTCCTTT GCTCAGCCGT TCTGGTTCGG GCCCTGCCAC ACCCCTTGCC 
CCTCGCAAGA AAGTTGTCGT GCCAACTGAA TATCAAGACA CAGTACCAGG AGAGTTTGAG 
GAGAAGATTA AGCAACCCAA ATCCTCTGGG ATGTCTCAGA GTAGTGCTCA AGACTCTCGA 
CCCCAAACCC CTGTCAGTGA GTATTCCCGT AAGGAGCTTA CACTTCGGCC CTCACCAAAA 
CTGACAAGAG CTAGCTCCAA AATCTTTGAG AAGGTGCGAG TCTTCGAAGA GCGCAGAAGA 
AGCATTGATA ACCCTGAAGG ATCTATATCT GGACGTTCTT GGGCTGGATT TAACAGAGCT 
TCATCTATCG ACTCTGATGA AGGAGGGAGC AGGTTGGGCA TCTCCAGGGA GAGCTCCAAA 
GAAGACCTTC GTGAGGCTTT GAAGGCAGAT GCAGCCCAAC GAAGGACCAT GTTTAAACAG 
CGGGCTGCTT CCCTGGAGGA CAGACCTCGT TACACCCAGA AGGTGCAGGA CATTGAAAAC 
AAGTTCACAG AGGAGCTCCA GCGCATCAAA AAGCTTGTCG GCAAACCACA CATGAAGAAA 
TCCTTCTCAA CAGAGCAGCT CTCTACTTGT CACAGAAGCA GGCAGCCTGT AAGAAAGTTA 
GAGCCCATCC CACCTCAGGT CCTTCAAAAG CTGCAGGATA GAGAACGTGC TCAGCTAGCT 
CAGCTAGAGC AAGAGATGAG AGAGCGAGAC CAGGCCAAAG AACGATCACC TCCGAAATCA 
CCGTCTCGCA GAAGGAAAGA CCATCTGGCA CAACAACCAC CAGAAAGAGC TGAGACCAAG 
GTGGTAAGCA CAACTGGACT TGAACCATCC CCTCCAGAAG CCATGTCACT CTCTGATTTA 
CCAGGTCAAA GATCTCCAAG ATTTCGGGGC CCAAGCCCAA GCAGAGATAG TACACGAAGG 
TCTCCAACCG TAGAGATTAG TGCCATGGCT GAGGAGCGCC CTCGAGGAAG AGCTGAATCT 
CCTTCAAGAA ATGTTTTGGA GATGACGTTA CGCAAAGTGG AGCCAAGACC TGCAAGTCCA 
CTGGTAAAGA GGGTGGTTCG TGTTCAAGAA GTTCCACAAG CAAACGATCA GCCTATGCAT 
AGAAAGACGC CAATAGAGGT CTCGCTAAGA AAACTGGAAA GAAGGCCTGA AAGTCCCCTG 
GTGCAAGGAG AAACCGTAGC CATTCAAGAC TTCCCTGTTC AAGCTCCCCC CAAACCTCCA 
AGAGTTTCCT CATCTTCCTC GTCTGAGGAG AAGATGGAGC TGGAAGTGAC TCCTCCACCA 
CCCCCACTAA AACTCACTAT ACCAAAAATT ATTGTTGAAG AAGAGCCCAT GGAGACAGAC 
ACACCTGTTG AGAAGACTGA AAAGGCTGTG AAAGTTGGCA CTGGTAAAGA TGAAAAACAT 
CAAAGATCCA GAGGAAGAGG ACGCAGACAA AGACCAATGT CCCCTGAATT AGAATCTTCG 
GACGACTCCT ATGTGTCTGC AGGAGAGGAT CCACTAGAAG CTCCTGTGTT CGAGTTTCCA 
CTTCAGGACA CTGTGGCCTC CACTGGAACG GAGGTGCTAT TAAAATGCAT CATCAGTGGC 
ACCCCTCTCC CTGAAGTTAT ATGGAAGAAA GACAACACTG AGGTCAAGAA CAGTCCCACT 
CATGTTGTAA AGATAGAGGG GGAACGCCAC TCCTTGCTCA TCAAATGGAC AAAGCCAAGT 
GATGCAGGAA CCTACACAGT TACCGCTGTC AATGAGGTCG GTGAGGTGTC CAGCAGTGCT 
ACACTTTTCA TCAAACCAGA GCCCAGTCAG GACCATCGTG GTAACCTTGG TGTCCCTATG 
GATGTCAGCA GTCCCATCAC ATCTGATGAG GAGTATCTCA GTCCACTGGA AGAGAGCATG 
GACTTCTCCT CCTACAGGGG ACCGGAGCCA AGAAAGATCG TGGACACTCG ATTTAAGGAG 
CCTCCAGCCT TTCTGGCTGT TATTGGAGAC CAAAGCGTAA TAGAGGGTCA GGAGGTGACC 
ATGTCTGTTC TAGTGACCGG ACAACCTAAA CCTATGCTTT ACTGGCTGAG AGACAGAGTG 
ACCATTAAAA CCGGAACACG GCATATCGTC CAAGAGACAG AGGAGGGCAA CTTTGAAATG 
ATAATTAAAT CAGCACAGAG GTCAGACACC GGTGTTTACA CCTGCAAGAT CATCAATGAA 
TATGGAACTA AGCAATGTGA AGGCAAACTG GAGGTGAAAG CTCGTCCAGT AGAGCCAGGT 
CTGGCCATCA TTCGGCCAGT GAGGGACGTG ATGGTGAAGG CAGGGGAGAC CGCTCTGTTT 
GAGTGTCATG TGATCGGCCC GCAGGACACA GATGTTGACT GGCTGTCAGA TGGGAAACTC 
ATCCAGCCTG CTTTGTTGAA CTGCAAGATG CATTTTGATG GGAAAAGGTG CAGGCTGCTT 
CTAAACTCTG TACATGAGGA TGACAGTGGA ACGTACACCT GCAAACTGAG CACAGCTAAA 
GAGGAACTGA CCTCCAGTGC CAAGCTAAAA GTCATAGCAT CCATAGAGCC CCTTTTCACT 
CGTAAGCTGG ATGTGCTGGA AGTTATCGAG GGTCGTAATG CACGATTTGA CTGCAAGGTC 
AGCGGGACGC CGCCGCCTCA AGTCATATGG AGCCACTTCG ATCGTCCGCT CGAGGAAAAC 
GAAGATATCA GGATCTTAAA AGAAGGAGGA CGGCACTCAT TAATCATCTC TCATGTTAGT 
AATGAAGATG AGGGCCTCTA CACGGTTGCA GCGCGAAACT CACACGGGGA AGACGAATGT 
GCAGCGGAGC TCTATGTGCA GGAGCCCAGA CCAGCAATAT CTTCTCAAAT AGCGAAGCTG 
GAAAAAATGC CTTCCATTCC CGAAGAACCC GAAGTTCCTG AAAATGAGAT TGAGCGTTTC 
ACCATGCCTG ACTTCGTAAA GCCGCTTTAT GATATGGATG TAGTGGAGGG CAGAGAGGCT 
GTGCTCAGGT GCAAAGTGGC CGGCTTGCCG TACCCCACCA TAACATGGTT TCACAATGGC 
AAGAGAATTG ACAGCACGGA GGACAGAAAG ATGACACAGT TCAGAGATGT TCACAGCCTG 
GTGGTCCGCT GTGTTTGTCA TGCTCACGGA GGGGTTTACA AGTGTGTCAT ATCCAATAAA 
GTGGGCAAGG CCACCTGCTA TTCTCATCTT TACGTGACAG ACATCCTTCC TGACCCTCCA 
GATGGTGCCC CAGTCGTTGA GTCCATCACT GGCAAGACTA TCACGCTAAG CTGGAAGAAA 
CCCAAAAGAC TGGATCCGTC CATTGACCCC AGCTCCCTGA TGTATGCCAT TCAGCAACAG 
GCCCTGGGGT CGATCCAGTG GACCATCATC GCTTCTTGCC TGAAACAGAC CACCTATACC 
ATCAGCAATC TGTCTAAAGG TGTCCGCTAC GCCTTCAGGG TGCTATCAAT CACCAGCAAG 
GCCTTTAGCA AGCCGTCGCC TGCCACCGAG CCAATGCAGC TGCTAGATAG AGGTCCGTAC 
GTCCAGGAGG CTCCGGTAAT CATTGACAAG CCAGATATCG TGTATATGGT GGAGAAGCAG 
CCCTTGAGCA TCACAGTCAC TCTCAACCAC GTCAACGCAT CGGTCACATG GAAGAGAGGA 
GGAGTGACAT TATCTAACCG ACCGGGTCTT TTTGAGTTGA GCATGCCTGA TGATGACCAG 
CACACACTGA AGCTGTGTAA GGTGAAGAGC AGTGATGTTG GCCAGCTCAC CTGCATTGCC 
AACAACAAGT ACGGCAGCGA CTCCTGTGTC CTCACCCTGG AGATGGCAGT GGCACCAACT 
TTTGAGACAA TTATGGAAGA CCTGGATGTG AATGTAGGCG AGACTCCTCG ATTTGCAGTG 
GTGGTTGAAG GAAAGCCCGT CCCAGACATC CTCTGGTACA AGGGAGACAC CCTTCTGTCC 
GAAAGCAGCC ATTTCACATT TGTGTATGAT GACAATGAAT GTTCTCTAGT GGTGCTCAAC 
ACTCAAGCAG ACGACTCAGG AGTGTACACT TGTACTGCGA AGAATCTGGC CGGTTCTGTG 
TCCTGCAAGG CTGAGCTGAC CGTCCACACC ACCAAACAGT ACAAAGACGA CCCGATGGAG 
GATGAGGCGT CCATCCTCAG AAAGATGCGG AGGCTCACTG ATTACTATGA TGTGCACAAG 
GAAATTGGGA GAGGGGCTTT CTCATATGTG AAGCGGGTGA TACAGAAGGC AGGAAAGCTG 
GAATACGCAG CCAAGTTTAT TTCTGCTCGG GCTAAACGAA AGGCATCCGC TCTGAGAGAA 
CTGAACATCC TCTCTCATCT GGACCATGAG AGGATCCTCT ATTTTCATGA TGCCTTTGAG 
AAGAAGAATG CTGTCATCAT CATTACAGAG CTATGCCATG AGGAGCTTCT CGATAGGCTT 
ACAAAGAAAT CAACCATCTT GGAGTCCGAG ATCCGATCCA GCGTGAGGCA GCTGTTGGAG 
GGTATTAACT ATCTCCATCA GCTTGACATA CTTCATCTAG ACATCAAGCC TGATAATATC 
CTCATGGCAG ACCACAGTAG CGATCAGATC CGCATATGTG ATTTTGGAAA TGCTGTAAAG 
TTCATGCCTG ATGAGGCACA GTACTGCAAA TATGGCACTC CAGAATTTGT CGCCCCAGAG 
ATTGTTAACC AGACACCCGT CTCCAAGGCA ACGGACATCT GGCCCATTGG TGTTCTGACA 
TACTTATGTC TTACTGGAGT TTCTCCTTTT GCTGGGGAAA ACGACCGCAG CTCTGTTCTC 
AACATCAGGA ATTATAATGT GGCTTTTGAA GAAAGCATGT TTACAGACCT CTGCCATGAA 
GCCAAGGGAT TCGTCATCAA GCTGCTGGTG GCTGATAGAT TGAGGCCTGA TGCTAATGAA 
TGTCTTCGCC ACCCCTGGTT CAAGACCCTC AACAAGGGTA AAAGCATCAG TACAGAGTCC 
CTTAAAAAGT TTTTGTCCAG AAGAAAATGG CAGCGATCGC TTATCAGCTA CAAATCTAAA 
ATGGTGATGA GGTCTGTACC TGAGCTACTG GATGACTCCT CCAGCCATAT TTCCATTGCT 
GTTCCGCGAC ATCTGAAAAA AGGCTCACCA CCACTTTCAT CATCTTCAGA TTCAGATGAA 
GACATCGATG AGTTGCCTTT TATTCCCATG CCACTCAACG TGGAATTCTC AGGATCTAGA 
ATGTCACTGA CCGAAATTGT AGGTGATGAT GACATGACAG GTAAATTGAA TGGAAATGCT 
GAGAGGCCTG CTTCACTCCA TCCGAAGTCC GAGCCAATGG AAGTGGAGAT GTCAGAGAGA 
GAAGGTGGAG ATCCATCTGC TAGGGGTAGA GCCAAGAAGA GGACTACAAC AGAAGATGAA 
AAAGGGTCGT CTGATGAGGA ACCATCACAA CTGGCCAAAA GAGCAGAACT GGCAAAGAGA 
CCAATGCGCA GAGGTTCAAG CATGGAGTCA GATGAGCCAG AGGGAGGACG TCGCAGAGGA 
GAGTTACGCA GAGGGAGTTC GGCTGACAGT GCCTTACAGC TCCATATCAA ACCAGAGGAG 
GGCACAGAAG AAAGTCCTAC TGATGGAAGT AGAATTCTAC AAAAGTCTGT ATCTATGGAG 
CTTCCAAGGA GAAGCCCAAG TCCAGGTGCT GCAAAGCTTA GCCAGGAAGA CTATGCTCTG 
AAACTGGAAC TGATGAGGCA GCGACTACTC AGGGGTGGCT CTGCTGACAA CAAGATGAGT 
GGATTACGAG GCCCATTGCT GGAGACACTA GGTATGGGAG ATGAGAAGCG GTTTGTGCGG 
GGATCTCGTC TAGGTAACCC TCCACTTGTT AGAGCGGCAT CTAGTGAGTC ACCTAGAGAT 
GACATCCCAA AGACAAAGAT TCTCCGCAAG AGTGCTTCCT TTAGCCAGGG GGATTCAGAG 
CCCATGCCCC TGCACAGAAG AATCGGAGCA CCTCTAGAGA TTCCTCTAGC ACAAATAGAG 
GAGAGACGCC TACAGGAAGC AATATCCATG TCTGCTCTCA CAGAGCCTGT CAAAGATTCT 
CGCCCAATCA CTCCAAAACT TCAAATGCCT GAAGAAGAAA GACATGAAAA GAAGAAAACT 
GAAGAAAAAG AATCTGTCTA CAAGGACAAT AAACCTGAAG AGGTTATTGC TGTGTCAAAG 
ACTGAGGAAG AAGGAAGACG TGTTGATCAG AAATCTTGGG ATTCTGATGC ACAGAAAAAG 
GACCAGAGCA AGTCAGAGGA AACCAGGGAA GAGTCTGAAA AAGTGGCTGA AATTGAAAAA 
AAGACCTCTA TAGTGGACGA AACAATTGAG GAAGAGGAAA CAAGAAAAGA TGAGGAGCCA 
GAGAAAGAAA CTGAGCCTCC TACTCCAAAG ATTGAGGTTA AGGAATATGA GGGTGATGTA 
AAGGTATTAC CAAAGTCTCC AGAGTTTATC ATTTCCACAA ACTCAATCAT GGTGACCCCA 
ACAGCTGCCC CAGCATCCAG TCCCCAGAGT GTATCAACCT ATGTTACCCC ATCTCCCCCT 
GCACGCACAG TGCTCCCAGA TGGTAGGATG TCCGCATATG CCAGTATTAT GCAGACTATC 
ATGGTACCAT CTGTCCCAGC AAATACCCAA TCTGACACTG TTCCTATTCC TACACCAACT 
TCCACTCCAC CATCAGTTCC AGTCTATGCA CCCATTTCCA GACACATCTC AGAACCAACC 
CTTTCATCAA CTTCTTCATC CAAATCAGAT CTTCACCAAA CAACTGAGCA TCCTGCAGTC 
TTTTCTCGGG TTGCCTCCAC TGAACACCCT CCCAAAGAGC CAAGTCCTCC TAAGACACCC 
ACTCGCGCCT CCCCTAAGCG AGAGCCATCT CCTGGAAGCA ACATCCAGGA CCTTGCATCC 
GAGGAGGTTT TTGAAGCTCG CTTCAAGAAA CGTGAGTCTT CTCTAACTCG TAGTCTCAAA 
CGTTTCTCAT TTCAGAAGAC TGAAGACAAG CCCTCCACTG TTTCTCCTGA AACTGCTGAA 
GAAATGTATC GCCCTGGACC AACTGGTGCT CCTTTAGAGT TTGTGCCAAG GAGGTTGGAG 
GAGAAGTCCA AATCAGTACA AGATCTTCGA GAGGCAGAGA AGGACCCTGG GTTCATGAGG 
CGACTCTCCA TGCGCTTGAA AAGGACCCCA TCAGTGGAAA AGCAGGAGGA GAAGCTCAAA 
GAGGAGGAGG CTTCAGCACC CAGGCGTCGT CTTTCTTGGG CACTGGGACG GAGGGGATCC 
CAAGAGAAGA AGGAGATTGA GATGATGAGG TTGGATGGGG GTCCTGAGCC TCCCCCAGAG 
CCTGAGTCTA AAGCACCTTC TGAGTCACCT ATTCTTGCCA TGCGCAAGAA GATTGGCAGC 
ACCATGGCAA GCTTATCTAC AAGAATTAGG AGCCATTCTG AGGACAGGAA AGATGACAGT 
GAAGGCAAGA TGGAGACCAA GAGGACTCCT ATATTCAACA AACTAAGGCG TTCCACTTCT 
GAGGGGGGCA GTCTGAAGAG AATGGGTATC CCACAAAACC AACTGGCTGC TCAGTCAGGG 
CAAGGGACCT CATCAGAATC TCTTGATTCT ATGTCAAGCA TCCAGTCAGA ATCAGCTATT 
AAAAATGCAG AACTTGACCG CAGATCACGG TGGGACCGAT GGGGCTTGAC TCGAGGGAAA 
AAGGACAAGA CAGTTTCCCA ACCAGACATC CCAGCTTCCA TTGCTAGAGA GAATGGTTCT 
CTACGGTCTC GCCAATATAC ACGTCTAACA TCTGACTTCC CTCCGGTGTT CCATATCAAA 
CTGAGGGACC ATGTACTCTT GGAGGGAGAT CCTGTCACTC TCAGCTGCCT CCCTGCTGGC 
AGTCCTCACC CACACATCTC ATGGATGAAA GACAAGAAGC CACTTGAGAT TGACCCCAGG 
ATGAATATGA TATCTTGCCC CGATGGGAGA CAACTGCTGA TGATTATGAA GACCACCAAG 
AAGGATGCAG GCCTCTATGA ATGTGTGGCT GCAAACCCAC TGGCCACAGT CACCAGCTCA 
TGCGTTGTGT CCCTCGCTCG TCTGCCTAAT AGTCCTGGGA CACCCGAGGT TCCTCAGAAG 
TATAAAAACA CTGCTCTAGT GGTATGGAGA CCCTCAGACA CTACGGCACC CTGCACCTAC 
TCTCTGGAGA GGAAGACAGA GGGTGAGAAT AACTGGCTGA TAGTCGCCAC AGGTGTTGCT 
GACTGTTACT ATAACGTGTT GGATCTTCCG GCCGGCGCTT CATACAGGTT CAGAGTGGCC 
TGCGTCAATA AGGCTGGTCA GGGACCTTAC AGCAGCTTGT CTGAAGTAGT GGTCCTGGAC 
TCAACAGAGC CCCAGAAGTC CAGTGGCACA GTTGTAGTCA AAACGCTTTC ATCAGCTCCC 
GCTCCTGCAG TGGTAACGTC CACCATGACC CTTCCTGCAA TGAAGCCTGC TGCAATAAAA 
CCAGCACCAT CAACAAATTC AGCGCCCTCT ACAGTCCAAC CGGTAAAGCC TGCGCCTCCT 
CCTGCATCAT CAACACCTAC CTATCGTCCC GTGCCTGACA TTCGAACTCC ATCGCCTGGC 
ACTCCAACCC CTGCCAAAGC CAAAAGCACC CTCAACATTA CTATGGCCAA ACCCTCCCCA 
GCATCCCCTG CAGCTCAACC ACAACCACAA CCTCCACCCA AACCCTCGCC TCCGGTCATT 
CTGCCCAAAC CTCAGACCCC AGTCAGTGTG GTTTCACCTC CATCCCAAAC TCCTCCAGTG 
TCACCTCCAC CTCTAGTGTC TCCTCCTCCC ATTATTGGTA AACCAATCTC CTCAGTGCCC 
ATGTATGTCC CTGCTACAAC CACCTCGACT CCAGTGACTC CGGTCACTCC TGTCACCCCA 
GTCACTCCAG TCACCCCAAA TACGCCCTCT CCTGCCCTGT CGCCTCCAGT GGTGTTGGTT 
ACAAGTCTGA GTCCTGTAGG GGAGGGAACT GGGACACCCA ATCGAATGAC CCCTAGTGGA 
AGAGCCACGC CTTCTGGACG CATCACTCCA TCAGGCCGAA GGACCCCTAT TGGAAAGCCT 
GGAGACTCAA CGCTGAGGCA AGGAGTCCCT CAGAAACCTT ACACCTTCAT GGATGAGAAA 
GCAAGGGGTC GCTTTGGTGT GATCAGAGAG TGTCGAGAAA ATGCCACTGG TAACCTGTAC 
ATGGCTAAGA TTGTGCCATA TGAGCCAGAG AGCAAGCAGA CTGTATTACA GGAATACGAC 
ATCCTGAAGT CTCTCCATCA TGAGAAGATC ATGGCTCTCC ACGAGGCCTA CGTCACTCCA 
CGCTACCTGG TGCTCATCTC TGAGTGCTGC TCTGGCAAAG AACTACTCCA CAGTCTCATT 
GACAGGTTCC GATACTCTGA GGATGATGTG GTTGCATATA TCGTACAGAT CCTTCAGGGT 
CTGGACTACC TGCACAGCCG GAGGATCCTG CATCTCGACA TCAAACCAGA AAACATCATC 
GTCACTTACA TGAATGTGGT GAAGATTATC GATTTTGGTA GTGCACAGAC CTTCAATCCT 
CTGTTCCTGA AGCAGTTCTC CCCACCTATT GGAACACTGG ATTATATGTC TCCAGAGATG 
CTGAAAGGAG ATGTGGTGGG TCCACCAGCT GACATCTGGA GCATTGGCAT ATTGACTTAT 
ATCATGCTTA GCGGGAGGTT GCCGTTCACA GAAAATGACC CTGCCGAGAC CGAGGCCAGA 
ATACAGGCAG CCAAGTTTGA CCTCAGTAAG CTTTACCAAA ACGTGTCCCA AAGTGCCTCT 
CTGTTCATCA AAAAGATCCT GTGCAGCTAC CCTTGGGCCC GTCCGACCAT CAAGGACTGC 
TTTACCAACT CGTGGCTGCA GGACGCATAT CTGATGCGCC TGCGAAGACA GACTCTCACA 
TTCACCACCA CACGCCTCAA AGAGTTCCTG GAGCAGCAGC AGCACATCAG AGCCCAAGAG 
GCAACAAAGC ACAAAGTCCT CCTGCGTTCC TACCAGAGCA ACCCACAAAC CCCATCCACC 
CCTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spegb ( XM_021479008.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334683.1 Danio rerio SPEG complex locus b (spegb), transcript variant X3, mRNA.

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CloneID	ODa144104
Clone ID Related Accession (Same CDS sequence)	XM_021479008.1
Accession Version	XM_021479008.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10458bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	striated muscle preferentially expressed protein kinase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAA TCTGGTCCAA GAAACGCTTT CAGAAAACCG GACACTCTAC CCGAACATTT 
GGGCGGTTCA CTCATGGTGT GTTTGGCTTT ATAGATAACA GCGAAGTTTT CCGCTCCTGT 
CGGAAACAAA GTTATGACTC TGAGACTACA GAAGACGAAG CCGCTGAGCC ACAAATACCC 
CTGGAGGCCA AAGAAGGAGC CAGAGGTTTT CAGGATGAGG CCCAACGAGT GCCTGCAGGT 
GAGAGAATGA TGGATGTCGC CCCAGATGCG AGCAATCGCT GGGCCGCCAG TGAAAGCACA 
GTGTTGGAGA GAGAAGCTCT AGCCCTGGGA TCGAGAGCCC CAGGGCCGCA GTCTGCCAGG 
AGCCCATCTG ACGATGTTTT GAGGGATTCT CCCCCTCAAG TTCTACCACC CCCTTCCCCC 
AAATTTGGCC GCTCTACTGC CTCTCCTTTG CTCAGCCGTT CTGGTTCGGG CCCTGCCACA 
CCCCTTGCCC CTCGCAAGAA AGTTGTCGTG CCAACTGAAT ATCAAGACAC AGTACCAGGA 
GAGTTTGAGG AGAAGATTAA GCAACCCAAA TCCTCTGGGA TGTCTCAGAG TAGTGCTCAA 
GACTCTCGAC CCCAAACCCC TGTCAGTGAG TATTCCCGTA AGGAGCTTAC ACTTCGGCCC 
TCACCAAAAC TGACAAGAGC TAGCTCCAAA ATCTTTGAGA AGGTGCGAGT CTTCGAAGAG 
CGCAGAAGAA GCATTGATAA CCCTGAAGGA TCTATATCTG GACGTTCTTG GGCTGGATTT 
AACAGAGCTT CATCTATCGA CTCTGATGAA GGAGGGAGCA GGTTGGGCAT CTCCAGGGAG 
AGCTCCAAAG AAGACCTTCG TGAGGCTTTG AAGGCAGATG CAGCCCAACG AAGGACCATG 
TTTAAACAGC GGGCTGCTTC CCTGGAGGAC AGACCTCGTT ACACCCAGAA GGTGCAGGAC 
ATTGAAAACA AGTTCACAGA GGAGCTCCAG CGCATCAAAA AGCTTGTCGG CAAACCACAC 
ATGAAGAAAT CCTTCTCAAC AGAGCAGCTC TCTACTTGTC ACAGAAGCAG GCAGCCTGTA 
AGAAAGTTAG AGCCCATCCC ACCTCAGGTC CTTCAAAAGC TGCAGGATAG AGAACGTGCT 
CAGCTAGCTC AGCTAGAGCA AGAGATGAGA GAGCGAGACC AGGCCAAAGA ACGATCACCT 
CCGAAATCAC CGTCTCGCAG AAGGAAAGAC CATCTGGCAC AACAACCACC AGAAAGAGCT 
GAGACCAAGG TGGTAAGCAC AACTGGACTT GAACCATCCC CTCCAGAAGC CATGTCACTC 
TCTGATTTAC CAGGTCAAAG ATCTCCAAGA TTTCGGGGCC CAAGCCCAAG CAGAGATAGT 
ACACGAAGGT CTCCAACCGT AGAGATTAGT GCCATGGCTG AGGAGCGCCC TCGAGGAAGA 
GCTGAATCTC CTTCAAGAAA TGTTTTGGAG ATGACGTTAC GCAAAGTGGA GCCAAGACCT 
GCAAGTCCAC TGGTAAAGAG GGTGGTTCGT GTTCAAGAAG TTCCACAAGC AAACGATCAG 
CCTATGCATA GAAAGACGCC AATAGAGGTC TCGCTAAGAA AACTGGAAAG AAGGCCTGAA 
AGTCCCCTGG TGCAAGGAGA AACCGTAGCC ATTCAAGACT TCCCTGTTCA AGCTCCCCCC 
AAACCTCCAA GAGTTTCCTC ATCTTCCTCG TCTGAGGAGA AGATGGAGCT GGAAGTGACT 
CCTCCACCAC CCCCACTAAA ACTCACTATA CCAAAAATTA TTGTTGAAGA AGAGCCCATG 
GAGACAGACA CACCTGTTGA GAAGACTGAA AAGGCTGTGA AAGTTGGCAC TGGTAAAGAT 
GAAAAACATC AAAGATCCAG AGGAAGAGGA CGCAGACAAA GACCAATGTC CCCTGAATTA 
GAATCTTCGG ACGACTCCTA TGTGTCTGCA GGAGAGGATC CACTAGAAGC TCCTGTGTTC 
GAGTTTCCAC TTCAGGACAC TGTGGCCTCC ACTGGAACGG AGGTGCTATT AAAATGCATC 
ATCAGTGGCA CCCCTCTCCC TGAAGTTATA TGGAAGAAAG ACAACACTGA GGTCAAGAAC 
AGTCCCACTC ATGTTGTAAA GATAGAGGGG GAACGCCACT CCTTGCTCAT CAAATGGACA 
AAGCCAAGTG ATGCAGGAAC CTACACAGTT ACCGCTGTCA ATGAGGTCGG TGAGGTGTCC 
AGCAGTGCTA CACTTTTCAT CAAACCAGAG CCCAGTCAGG ACCATCGTGG TAACCTTGGT 
GTCCCTATGG ATGTCAGCAG TCCCATCACA TCTGATGAGG AGTATCTCAG TCCACTGGAA 
GAGAGCATGG ACTTCTCCTC CTACAGGGGA CCGGAGCCAA GAAAGATCGT GGACACTCGA 
TTTAAGGAGC CTCCAGCCTT TCTGGCTGTT ATTGGAGACC AAAGCGTAAT AGAGGGTCAG 
GAGGTGACCA TGTCTGTTCT AGTGACCGGA CAACCTAAAC CTATGCTTTA CTGGCTGAGA 
GACAGAGTGA CCATTAAAAC CGGAACACGG CATATCGTCC AAGAGACAGA GGAGGGCAAC 
TTTGAAATGA TAATTAAATC AGCACAGAGG TCAGACACCG GTGTTTACAC CTGCAAGATC 
ATCAATGAAT ATGGAACTAA GCAATGTGAA GGCAAACTGG AGGTGAAAGC TCGTCCAGTA 
GAGCCAGGTC TGGCCATCAT TCGGCCAGTG AGGGACGTGA TGGTGAAGGC AGGGGAGACC 
GCTCTGTTTG AGTGTCATGT GATCGGCCCG CAGGACACAG ATGTTGACTG GCTGTCAGAT 
GGGAAACTCA TCCAGCCTGC TTTGTTGAAC TGCAAGATGC ATTTTGATGG GAAAAGGTGC 
AGGCTGCTTC TAAACTCTGT ACATGAGGAT GACAGTGGAA CGTACACCTG CAAACTGAGC 
ACAGCTAAAG AGGAACTGAC CTCCAGTGCC AAGCTAAAAG TCATAGCATC CATAGAGCCC 
CTTTTCACTC GTAAGCTGGA TGTGCTGGAA GTTATCGAGG GTCGTAATGC ACGATTTGAC 
TGCAAGGTCA GCGGGACGCC GCCGCCTCAA GTCATATGGA GCCACTTCGA TCGTCCGCTC 
GAGGAAAACG AAGATATCAG GATCTTAAAA GAAGGAGGAC GGCACTCATT AATCATCTCT 
CATGTTAGTA ATGAAGATGA GGGCCTCTAC ACGGTTGCAG CGCGAAACTC ACACGGGGAA 
GACGAATGTG CAGCGGAGCT CTATGTGCAG GAGCCCAGAC CAGCAATATC TTCTCAAATA 
GCGAAGCTGG AAAAAATGCC TTCCATTCCC GAAGAACCCG AAGTTCCTGA AAATGAGATT 
GAGCGTTTCA CCATGCCTGA CTTCGTAAAG CCGCTTTATG ATATGGATGT AGTGGAGGGC 
AGAGAGGCTG TGCTCAGGTG CAAAGTGGCC GGCTTGCCGT ACCCCACCAT AACATGGTTT 
CACAATGGCA AGAGAATTGA CAGCACGGAG GACAGAAAGA TGACACAGTT CAGAGATGTT 
CACAGCCTGG TGGTCCGCTG TGTTTGTCAT GCTCACGGAG GGGTTTACAA GTGTGTCATA 
TCCAATAAAG TGGGCAAGGC CACCTGCTAT TCTCATCTTT ACGTGACAGA CATCCTTCCT 
GACCCTCCAG ATGGTGCCCC AGTCGTTGAG TCCATCACTG GCAAGACTAT CACGCTAAGC 
TGGAAGAAAC CCAAAAGACT GGATCCGTCC ATTGACCCCA GCTCCCTGAT GTATGCCATT 
CAGCAACAGG CCCTGGGGTC GATCCAGTGG ACCATCATCG CTTCTTGCCT GAAACAGACC 
ACCTATACCA TCAGCAATCT GTCTAAAGGT GTCCGCTACG CCTTCAGGGT GCTATCAATC 
ACCAGCAAGG CCTTTAGCAA GCCGTCGCCT GCCACCGAGC CAATGCAGCT GCTAGATAGA 
GGTCCGTACG TCCAGGAGGC TCCGGTAATC ATTGACAAGC CAGATATCGT GTATATGGTG 
GAGAAGCAGC CCTTGAGCAT CACAGTCACT CTCAACCACG TCAACGCATC GGTCACATGG 
AAGAGAGGAG GAGTGACATT ATCTAACCGA CCGGGTCTTT TTGAGTTGAG CATGCCTGAT 
GATGACCAGC ACACACTGAA GCTGTGTAAG GTGAAGAGCA GTGATGTTGG CCAGCTCACC 
TGCATTGCCA ACAACAAGTA CGGCAGCGAC TCCTGTGTCC TCACCCTGGA GATGGCAGTG 
GCACCAACTT TTGAGACAAT TATGGAAGAC CTGGATGTGA ATGTAGGCGA GACTCCTCGA 
TTTGCAGTGG TGGTTGAAGG AAAGCCCGTC CCAGACATCC TCTGGTACAA GGGAGACACC 
CTTCTGTCCG AAAGCAGCCA TTTCACATTT GTGTATGATG ACAATGAATG TTCTCTAGTG 
GTGCTCAACA CTCAAGCAGA CGACTCAGGA GTGTACACTT GTACTGCGAA GAATCTGGCC 
GGTTCTGTGT CCTGCAAGGC TGAGCTGACC GTCCACACCA CCAAACAGTA CAAAGACGAC 
CCGATGGAGG ATGAGGCGTC CATCCTCAGA AAGATGCGGA GGCTCACTGA TTACTATGAT 
GTGCACAAGG AAATTGGGAG AGGGGCTTTC TCATATGTGA AGCGGGTGAT ACAGAAGGCA 
GGAAAGCTGG AATACGCAGC CAAGTTTATT TCTGCTCGGG CTAAACGAAA GGCATCCGCT 
CTGAGAGAAC TGAACATCCT CTCTCATCTG GACCATGAGA GGATCCTCTA TTTTCATGAT 
GCCTTTGAGA AGAAGAATGC TGTCATCATC ATTACAGAGC TATGCCATGA GGAGCTTCTC 
GATAGGCTTA CAAAGAAATC AACCATCTTG GAGTCCGAGA TCCGATCCAG CGTGAGGCAG 
CTGTTGGAGG GTATTAACTA TCTCCATCAG CTTGACATAC TTCATCTAGA CATCAAGCCT 
GATAATATCC TCATGGCAGA CCACAGTAGC GATCAGATCC GCATATGTGA TTTTGGAAAT 
GCTGTAAAGT TCATGCCTGA TGAGGCACAG TACTGCAAAT ATGGCACTCC AGAATTTGTC 
GCCCCAGAGA TTGTTAACCA GACACCCGTC TCCAAGGCAA CGGACATCTG GCCCATTGGT 
GTTCTGACAT ACTTATGTCT TACTGGAGTT TCTCCTTTTG CTGGGGAAAA CGACCGCAGC 
TCTGTTCTCA ACATCAGGAA TTATAATGTG GCTTTTGAAG AAAGCATGTT TACAGACCTC 
TGCCATGAAG CCAAGGGATT CGTCATCAAG CTGCTGGTGG CTGATAGATT GAGGCCTGAT 
GCTAATGAAT GTCTTCGCCA CCCCTGGTTC AAGACCCTCA ACAAGGGTAA AAGCATCAGT 
ACAGAGTCCC TTAAAAAGTT TTTGTCCAGA AGAAAATGGC AGCGATCGCT TATCAGCTAC 
AAATCTAAAA TGGTGATGAG GTCTGTACCT GAGCTACTGG ATGACTCCTC CAGCCATATT 
TCCATTGCTG TTCCGCGACA TCTGAAAAAA GGCTCACCAC CACTTTCATC ATCTTCAGAT 
TCAGATGAAG ACATCGATGA GTTGCCTTTT ATTCCCATGC CACTCAACGT GGAATTCTCA 
GGATCTAGAA TGTCACTGAC CGAAATTGTA GGTGATGATG ACATGACAGG TAAATTGAAT 
GGAAATGCTG AGAGGCCTGC TTCACTCCAT CCGAAGTCCG AGCCAATGGA AGTGGAGATG 
TCAGAGAGAG AAGGTGGAGA TCCATCTGCT AGGGGTAGAG CCAAGAAGAG GACTACAACA 
GAAGATGAAA AAGGGTCGTC TGATGAGGAA CCATCACAAC TGGCCAAAAG AGCAGAACTG 
GCAAAGAGAC CAATGCGCAG AGGTTCAAGC ATGGAGTCAG ATGAGCCAGA GGGAGGACGT 
CGCAGAGGAG AGTTACGCAG AGGGAGTTCG GCTGACAGTG CCTTACAGCT CCATATCAAA 
CCAGAGGAGG GCACAGAAGA AAGTCCTACT GATGGAAGTA GAATTCTACA AAAGTCTGTA 
TCTATGGAGC TTCCAAGGAG AAGCCCAAGT CCAGGTGCTG CAAAGCTTAG CCAGGAAGAC 
TATGCTCTGA AACTGGAACT GATGAGGCAG CGACTACTCA GGGGTGGCTC TGCTGACAAC 
AAGATGAGTG GATTACGAGG CCCATTGCTG GAGACACTAG GTATGGGAGA TGAGAAGCGG 
TTTGTGCGGG GATCTCGTCT AGGTAACCCT CCACTTGTTA GAGCGGCATC TAGTGAGTCA 
CCTAGAGATG ACATCCCAAA GACAAAGATT CTCCGCAAGA GTGCTTCCTT TAGCCAGGGG 
GATTCAGAGC CCATGCCCCT GCACAGAAGA ATCGGAGCAC CTCTAGAGAT TCCTCTAGCA 
CAAATAGAGG AGAGACGCCT ACAGGAAGCA ATATCCATGT CTGCTCTCAC AGAGCCTGTC 
AAAGATTCTC GCCCAATCAC TCCAAAACTT CAAATGCCTG AAGAAGAAAG ACATGAAAAG 
AAGAAAACTG AAGAAAAAGA ATCTGTCTAC AAGGACAATA AACCTGAAGA GGTTATTGCT 
GTGTCAAAGA CTGAGGAAGA AGGAAGACGT GTTGATCAGA AATCTTGGGA TTCTGATGCA 
CAGAAAAAGG ACCAGAGCAA GTCAGAGGAA ACCAGGGAAG AGTCTGAAAA AGTGGCTGAA 
ATTGAAAAAA AGACCTCTAT AGTGGACGAA ACAATTGAGG AAGAGGAAAC AAGAAAAGAT 
GAGGAGCCAG AGAAAGAAAC TGAGCCTCCT ACTCCAAAGA TTGAGGTTAA GGAATATGAG 
GGTGATGTAA AGGTATTACC AAAGTCTCCA GAGTTTATCA TTTCCACAAA CTCAATCATG 
GTGACCCCAA CAGCTGCCCC AGCATCCAGT CCCCAGAGTG TATCAACCTA TGTTACCCCA 
TCTCCCCCTG CACGCACAGT GCTCCCAGAT GGTAGGATGT CCGCATATGC CAGTATTATG 
CAGACTATCA TGGTACCATC TGTCCCAGCA AATACCCAAT CTGACACTGT TCCTATTCCT 
ACACCAACTT CCACTCCACC ATCAGTTCCA GTCTATGCAC CCATTTCCAG ACACATCTCA 
GAACCAACCC TTTCATCAAC TTCTTCATCC AAATCAGATC TTCACCAAAC AACTGAGCAT 
CCTGCAGTCT TTTCTCGGGT TGCCTCCACT GAACACCCTC CCAAAGAGCC AAGTCCTCCT 
AAGACACCCA CTCGCGCCTC CCCTAAGCGA GAGCCATCTC CTGGAAGCAA CATCCAGGAC 
CTTGCATCCG AGGAGGTTTT TGAAGCTCGC TTCAAGAAAC GTGAGTCTTC TCTAACTCGT 
AGTCTCAAAC GTTTCTCATT TCAGAAGACT GAAGACAAGC CCTCCACTGT TTCTCCTGAA 
ACTGCTGAAG AAATGTATCG CCCTGGACCA ACTGGTGCTC CTTTAGAGTT TGTGCCAAGG 
AGGTTGGAGG AGAAGTCCAA ATCAGTACAA GATCTTCGAG AGGCAGAGAA GGACCCTGGG 
TTCATGAGGC GACTCTCCAT GCGCTTGAAA AGGACCCCAT CAGTGGAAAA GCAGGAGGAG 
AAGCTCAAAG AGGAGGAGGC TTCAGCACCC AGGCGTCGTC TTTCTTGGGC ACTGGGACGG 
AGGGGATCCC AAGAGAAGAA GGAGATTGAG ATGATGAGGT TGGATGGGGG TCCTGAGCCT 
CCCCCAGAGC CTGAGTCTAA AGCACCTTCT GAGTCACCTA TTCTTGCCAT GCGCAAGAAG 
ATTGGCAGCA CCATGGCAAG CTTATCTACA AGAATTAGGA GCCATTCTGA GGACAGGAAA 
GATGACAGTG AAGGCAAGAT GGAGACCAAG AGGACTCCTA TATTCAACAA ACTAAGGCGT 
TCCACTTCTG AGGGGGGCAG TCTGAAGAGA ATGGGTATCC CACAAAACCA ACTGGCTGCT 
CAGTCAGGGC AAGGGACCTC ATCAGAATCT CTTGATTCTA TGTCAAGCAT CCAGTCAGAA 
TCAGCTATTA AAAATGCAGA ACTTGACCGC AGATCACGGT GGGACCGATG GGGCTTGACT 
CGAGGGAAAA AGGACAAGAC AGTTTCCCAA CCAGACATCC CAGCTTCCAT TGCTAGAGAG 
AATGGTTCTC TACGGTCTCG CCAATATACA CGTCTAACAT CTGACTTCCC TCCGGTGTTC 
CATATCAAAC TGAGGGACCA TGTACTCTTG GAGGGAGATC CTGTCACTCT CAGCTGCCTC 
CCTGCTGGCA GTCCTCACCC ACACATCTCA TGGATGAAAG ACAAGAAGCC ACTTGAGATT 
GACCCCAGGA TGAATATGAT ATCTTGCCCC GATGGGAGAC AACTGCTGAT GATTATGAAG 
ACCACCAAGA AGGATGCAGG CCTCTATGAA TGTGTGGCTG CAAACCCACT GGCCACAGTC 
ACCAGCTCAT GCGTTGTGTC CCTCGCTCGT CTGCCTAATA GTCCTGGGAC ACCCGAGGTT 
CCTCAGAAGT ATAAAAACAC TGCTCTAGTG GTATGGAGAC CCTCAGACAC TACGGCACCC 
TGCACCTACT CTCTGGAGAG GAAGACAGAG GGTGAGAATA ACTGGCTGAT AGTCGCCACA 
GGTGTTGCTG ACTGTTACTA TAACGTGTTG GATCTTCCGG CCGGCGCTTC ATACAGGTTC 
AGAGTGGCCT GCGTCAATAA GGCTGGTCAG GGACCTTACA GCAGCTTGTC TGAAGTAGTG 
GTCCTGGACT CAACAGAGCC CCAGAAGTCC AGTGGCACAG TTGTAGTCAA AACGCTTTCA 
TCAGCTCCCG CTCCTGCAGT GGTAACGTCC ACCATGACCC TTCCTGCAAT GAAGCCTGCT 
GCAATAAAAC CAGCACCATC AACAAATTCA GCGCCCTCTA CAGTCCAACC GGTAAAGCCT 
GCGCCTCCTC CTGCATCATC AACACCTACC TATCGTCCCG TGCCTGACAT TCGAACTCCA 
TCGCCTGGCA CTCCAACCCC TGCCAAAGCC AAAAGCACCC TCAACATTAC TATGGCCAAA 
CCCTCCCCAG CATCCCCTGC AGCTCAACCA CAACCACAAC CTCCACCCAA ACCCTCGCCT 
CCGGTCATTC TGCCCAAACC TCAGACCCCA GTCAGTGTGG TTTCACCTCC ATCCCAAACT 
CCTCCAGTGT CACCTCCACC TCTAGTGTCT CCTCCTCCCA TTATTGGTAA ACCAATCTCC 
TCAGTGCCCA TGTATGTCCC TGCTACAACC ACCTCGACTC CAGTGACTCC GGTCACTCCT 
GTCACCCCAG TCACTCCAGT CACCCCAAAT ACGCCCTCTC CTGCCCTGTC GCCTCCAGTG 
GTGTTGGTTA CAAGTCTGAG TCCTGTAGGG GAGGGAACTG GGACACCCAA TCGAATGACC 
CCTAGTGGAA GAGCCACGCC TTCTGGACGC ATCACTCCAT CAGGCCGAAG GACCCCTATT 
GGAAAGCCTG GAGACTCAAC GCTGAGGCAA GGAGTCCCTC AGAAACCTTA CACCTTCATG 
GATGAGAAAG CAAGGGGTCG CTTTGGTGTG ATCAGAGAGT GTCGAGAAAA TGCCACTGGT 
AACCTGTACA TGGCTAAGAT TGTGCCATAT GAGCCAGAGA GCAAGCAGAC TGTATTACAG 
GAATACGACA TCCTGAAGTC TCTCCATCAT GAGAAGATCA TGGCTCTCCA CGAGGCCTAC 
GTCACTCCAC GCTACCTGGT GCTCATCTCT GAGTGCTGCT CTGGCAAAGA ACTACTCCAC 
AGTCTCATTG ACAGGTTCCG ATACTCTGAG GATGATGTGG TTGCATATAT CGTACAGATC 
CTTCAGGGTC TGGACTACCT GCACAGCCGG AGGATCCTGC ATCTCGACAT CAAACCAGAA 
AACATCATCG TCACTTACAT GAATGTGGTG AAGATTATCG ATTTTGGTAG TGCACAGACC 
TTCAATCCTC TGTTCCTGAA GCAGTTCTCC CCACCTATTG GAACACTGGA TTATATGTCT 
CCAGAGATGC TGAAAGGAGA TGTGGTGGGT CCACCAGCTG ACATCTGGAG CATTGGCATA 
TTGACTTATA TCATGCTTAG CGGGAGGTTG CCGTTCACAG AAAATGACCC TGCCGAGACC 
GAGGCCAGAA TACAGGCAGC CAAGTTTGAC CTCAGTAAGC TTTACCAAAA CGTGTCCCAA 
AGTGCCTCTC TGTTCATCAA AAAGATCCTG TGCAGCTACC CTTGGGCCCG TCCGACCATC 
AAGGACTGCT TTACCAACTC GTGGCTGCAG GACGCATATC TGATGCGCCT GCGAAGACAG 
ACTCTCACAT TCACCACCAC ACGCCTCAAA GAGTTCCTGG AGCAGCAGCA GCACATCAGA 
GCCCAAGAGG CAACAAAGCA CAAAGTCCTC CTGCGTTCCT ACCAGAGCAA CCCACAAACC 
CCATCCACCC CTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334683.1
CDS	940..11397
Translation	MKKIWSKKRFQKTGHSTRTFGRFTHGVFGFIDNSEVFRSCRKQSYDSETTEDEAAEPQIPLEAKEGARGFQDEAQRVPAGERMMDVAPDASNRWAASESTVLEREALALGSRAPGPQSARSPSDDVLRDSPPQVLPPPSPKFGRSTASPLLSRSGSGPATPLAPRKKVVVPTEYQDTVPGEFEEKIKQPKSSGMSQSSAQDSRPQTPVSEYSRKELTLRPSPKLTRASSKIFEKVRVFEERRRSIDNPEGSISGRSWAGFNRASSIDSDEGGSRLGISRESSKEDLREALKADAAQRRTMFKQRAASLEDRPRYTQKVQDIENKFTEELQRIKKLVGKPHMKKSFSTEQLSTCHRSRQPVRKLEPIPPQVLQKLQDRERAQLAQLEQEMRERDQAKERSPPKSPSRRRKDHLAQQPPERAETKVVSTTGLEPSPPEAMSLSDLPGQRSPRFRGPSPSRDSTRRSPTVEISAMAEERPRGRAESPSRNVLEMTLRKVEPRPASPLVKRVVRVQEVPQANDQPMHRKTPIEVSLRKLERRPESPLVQGETVAIQDFPVQAPPKPPRVSSSSSSEEKMELEVTPPPPPLKLTIPKIIVEEEPMETDTPVEKTEKAVKVGTGKDEKHQRSRGRGRRQRPMSPELESSDDSYVSAGEDPLEAPVFEFPLQDTVASTGTEVLLKCIISGTPLPEVIWKKDNTEVKNSPTHVVKIEGERHSLLIKWTKPSDAGTYTVTAVNEVGEVSSSATLFIKPEPSQDHRGNLGVPMDVSSPITSDEEYLSPLEESMDFSSYRGPEPRKIVDTRFKEPPAFLAVIGDQSVIEGQEVTMSVLVTGQPKPMLYWLRDRVTIKTGTRHIVQETEEGNFEMIIKSAQRSDTGVYTCKIINEYGTKQCEGKLEVKARPVEPGLAIIRPVRDVMVKAGETALFECHVIGPQDTDVDWLSDGKLIQPALLNCKMHFDGKRCRLLLNSVHEDDSGTYTCKLSTAKEELTSSAKLKVIASIEPLFTRKLDVLEVIEGRNARFDCKVSGTPPPQVIWSHFDRPLEENEDIRILKEGGRHSLIISHVSNEDEGLYTVAARNSHGEDECAAELYVQEPRPAISSQIAKLEKMPSIPEEPEVPENEIERFTMPDFVKPLYDMDVVEGREAVLRCKVAGLPYPTITWFHNGKRIDSTEDRKMTQFRDVHSLVVRCVCHAHGGVYKCVISNKVGKATCYSHLYVTDILPDPPDGAPVVESITGKTITLSWKKPKRLDPSIDPSSLMYAIQQQALGSIQWTIIASCLKQTTYTISNLSKGVRYAFRVLSITSKAFSKPSPATEPMQLLDRGPYVQEAPVIIDKPDIVYMVEKQPLSITVTLNHVNASVTWKRGGVTLSNRPGLFELSMPDDDQHTLKLCKVKSSDVGQLTCIANNKYGSDSCVLTLEMAVAPTFETIMEDLDVNVGETPRFAVVVEGKPVPDILWYKGDTLLSESSHFTFVYDDNECSLVVLNTQADDSGVYTCTAKNLAGSVSCKAELTVHTTKQYKDDPMEDEASILRKMRRLTDYYDVHKEIGRGAFSYVKRVIQKAGKLEYAAKFISARAKRKASALRELNILSHLDHERILYFHDAFEKKNAVIIITELCHEELLDRLTKKSTILESEIRSSVRQLLEGINYLHQLDILHLDIKPDNILMADHSSDQIRICDFGNAVKFMPDEAQYCKYGTPEFVAPEIVNQTPVSKATDIWPIGVLTYLCLTGVSPFAGENDRSSVLNIRNYNVAFEESMFTDLCHEAKGFVIKLLVADRLRPDANECLRHPWFKTLNKGKSISTESLKKFLSRRKWQRSLISYKSKMVMRSVPELLDDSSSHISIAVPRHLKKGSPPLSSSSDSDEDIDELPFIPMPLNVEFSGSRMSLTEIVGDDDMTGKLNGNAERPASLHPKSEPMEVEMSEREGGDPSARGRAKKRTTTEDEKGSSDEEPSQLAKRAELAKRPMRRGSSMESDEPEGGRRRGELRRGSSADSALQLHIKPEEGTEESPTDGSRILQKSVSMELPRRSPSPGAAKLSQEDYALKLELMRQRLLRGGSADNKMSGLRGPLLETLGMGDEKRFVRGSRLGNPPLVRAASSESPRDDIPKTKILRKSASFSQGDSEPMPLHRRIGAPLEIPLAQIEERRLQEAISMSALTEPVKDSRPITPKLQMPEEERHEKKKTEEKESVYKDNKPEEVIAVSKTEEEGRRVDQKSWDSDAQKKDQSKSEETREESEKVAEIEKKTSIVDETIEEEETRKDEEPEKETEPPTPKIEVKEYEGDVKVLPKSPEFIISTNSIMVTPTAAPASSPQSVSTYVTPSPPARTVLPDGRMSAYASIMQTIMVPSVPANTQSDTVPIPTPTSTPPSVPVYAPISRHISEPTLSSTSSSKSDLHQTTEHPAVFSRVASTEHPPKEPSPPKTPTRASPKREPSPGSNIQDLASEEVFEARFKKRESSLTRSLKRFSFQKTEDKPSTVSPETAEEMYRPGPTGAPLEFVPRRLEEKSKSVQDLREAEKDPGFMRRLSMRLKRTPSVEKQEEKLKEEEASAPRRRLSWALGRRGSQEKKEIEMMRLDGGPEPPPEPESKAPSESPILAMRKKIGSTMASLSTRIRSHSEDRKDDSEGKMETKRTPIFNKLRRSTSEGGSLKRMGIPQNQLAAQSGQGTSSESLDSMSSIQSESAIKNAELDRRSRWDRWGLTRGKKDKTVSQPDIPASIARENGSLRSRQYTRLTSDFPPVFHIKLRDHVLLEGDPVTLSCLPAGSPHPHISWMKDKKPLEIDPRMNMISCPDGRQLLMIMKTTKKDAGLYECVAANPLATVTSSCVVSLARLPNSPGTPEVPQKYKNTALVVWRPSDTTAPCTYSLERKTEGENNWLIVATGVADCYYNVLDLPAGASYRFRVACVNKAGQGPYSSLSEVVVLDSTEPQKSSGTVVVKTLSSAPAPAVVTSTMTLPAMKPAAIKPAPSTNSAPSTVQPVKPAPPPASSTPTYRPVPDIRTPSPGTPTPAKAKSTLNITMAKPSPASPAAQPQPQPPPKPSPPVILPKPQTPVSVVSPPSQTPPVSPPPLVSPPPIIGKPISSVPMYVPATTTSTPVTPVTPVTPVTPVTPNTPSPALSPPVVLVTSLSPVGEGTGTPNRMTPSGRATPSGRITPSGRRTPIGKPGDSTLRQGVPQKPYTFMDEKARGRFGVIRECRENATGNLYMAKIVPYEPESKQTVLQEYDILKSLHHEKIMALHEAYVTPRYLVLISECCSGKELLHSLIDRFRYSEDDVVAYIVQILQGLDYLHSRRILHLDIKPENIIVTYMNVVKIIDFGSAQTFNPLFLKQFSPPIGTLDYMSPEMLKGDVVGPPADIWSIGILTYIMLSGRLPFTENDPAETEARIQAAKFDLSKLYQNVSQSASLFIKKILCSYPWARPTIKDCFTNSWLQDAYLMRLRRQTLTFTTTRLKEFLEQQQHIRAQEATKHKVLLRSYQSNPQTPSTPQ

Target ORF information:

RefSeq Version	XM_021479008.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SPEG complex locus b (spegb), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479008.1

ORF Insert Sequence:

ATGAAGAAAA TCTGGTCCAA GAAACGCTTT CAGAAAACCG GACACTCTAC CCGAACATTT 
GGGCGGTTCA CTCATGGTGT GTTTGGCTTT ATAGATAACA GCGAAGTTTT CCGCTCCTGT 
CGGAAACAAA GTTATGACTC TGAGACTACA GAAGACGAAG CCGCTGAGCC ACAAATACCC 
CTGGAGGCCA AAGAAGGAGC CAGAGGTTTT CAGGATGAGG CCCAACGAGT GCCTGCAGGT 
GAGAGAATGA TGGATGTCGC CCCAGATGCG AGCAATCGCT GGGCCGCCAG TGAAAGCACA 
GTGTTGGAGA GAGAAGCTCT AGCCCTGGGA TCGAGAGCCC CAGGGCCGCA GTCTGCCAGG 
AGCCCATCTG ACGATGTTTT GAGGGATTCT CCCCCTCAAG TTCTACCACC CCCTTCCCCC 
AAATTTGGCC GCTCTACTGC CTCTCCTTTG CTCAGCCGTT CTGGTTCGGG CCCTGCCACA 
CCCCTTGCCC CTCGCAAGAA AGTTGTCGTG CCAACTGAAT ATCAAGACAC AGTACCAGGA 
GAGTTTGAGG AGAAGATTAA GCAACCCAAA TCCTCTGGGA TGTCTCAGAG TAGTGCTCAA 
GACTCTCGAC CCCAAACCCC TGTCAGTGAG TATTCCCGTA AGGAGCTTAC ACTTCGGCCC 
TCACCAAAAC TGACAAGAGC TAGCTCCAAA ATCTTTGAGA AGGTGCGAGT CTTCGAAGAG 
CGCAGAAGAA GCATTGATAA CCCTGAAGGA TCTATATCTG GACGTTCTTG GGCTGGATTT 
AACAGAGCTT CATCTATCGA CTCTGATGAA GGAGGGAGCA GGTTGGGCAT CTCCAGGGAG 
AGCTCCAAAG AAGACCTTCG TGAGGCTTTG AAGGCAGATG CAGCCCAACG AAGGACCATG 
TTTAAACAGC GGGCTGCTTC CCTGGAGGAC AGACCTCGTT ACACCCAGAA GGTGCAGGAC 
ATTGAAAACA AGTTCACAGA GGAGCTCCAG CGCATCAAAA AGCTTGTCGG CAAACCACAC 
ATGAAGAAAT CCTTCTCAAC AGAGCAGCTC TCTACTTGTC ACAGAAGCAG GCAGCCTGTA 
AGAAAGTTAG AGCCCATCCC ACCTCAGGTC CTTCAAAAGC TGCAGGATAG AGAACGTGCT 
CAGCTAGCTC AGCTAGAGCA AGAGATGAGA GAGCGAGACC AGGCCAAAGA ACGATCACCT 
CCGAAATCAC CGTCTCGCAG AAGGAAAGAC CATCTGGCAC AACAACCACC AGAAAGAGCT 
GAGACCAAGG TGGTAAGCAC AACTGGACTT GAACCATCCC CTCCAGAAGC CATGTCACTC 
TCTGATTTAC CAGGTCAAAG ATCTCCAAGA TTTCGGGGCC CAAGCCCAAG CAGAGATAGT 
ACACGAAGGT CTCCAACCGT AGAGATTAGT GCCATGGCTG AGGAGCGCCC TCGAGGAAGA 
GCTGAATCTC CTTCAAGAAA TGTTTTGGAG ATGACGTTAC GCAAAGTGGA GCCAAGACCT 
GCAAGTCCAC TGGTAAAGAG GGTGGTTCGT GTTCAAGAAG TTCCACAAGC AAACGATCAG 
CCTATGCATA GAAAGACGCC AATAGAGGTC TCGCTAAGAA AACTGGAAAG AAGGCCTGAA 
AGTCCCCTGG TGCAAGGAGA AACCGTAGCC ATTCAAGACT TCCCTGTTCA AGCTCCCCCC 
AAACCTCCAA GAGTTTCCTC ATCTTCCTCG TCTGAGGAGA AGATGGAGCT GGAAGTGACT 
CCTCCACCAC CCCCACTAAA ACTCACTATA CCAAAAATTA TTGTTGAAGA AGAGCCCATG 
GAGACAGACA CACCTGTTGA GAAGACTGAA AAGGCTGTGA AAGTTGGCAC TGGTAAAGAT 
GAAAAACATC AAAGATCCAG AGGAAGAGGA CGCAGACAAA GACCAATGTC CCCTGAATTA 
GAATCTTCGG ACGACTCCTA TGTGTCTGCA GGAGAGGATC CACTAGAAGC TCCTGTGTTC 
GAGTTTCCAC TTCAGGACAC TGTGGCCTCC ACTGGAACGG AGGTGCTATT AAAATGCATC 
ATCAGTGGCA CCCCTCTCCC TGAAGTTATA TGGAAGAAAG ACAACACTGA GGTCAAGAAC 
AGTCCCACTC ATGTTGTAAA GATAGAGGGG GAACGCCACT CCTTGCTCAT CAAATGGACA 
AAGCCAAGTG ATGCAGGAAC CTACACAGTT ACCGCTGTCA ATGAGGTCGG TGAGGTGTCC 
AGCAGTGCTA CACTTTTCAT CAAACCAGAG CCCAGTCAGG ACCATCGTGG TAACCTTGGT 
GTCCCTATGG ATGTCAGCAG TCCCATCACA TCTGATGAGG AGTATCTCAG TCCACTGGAA 
GAGAGCATGG ACTTCTCCTC CTACAGGGGA CCGGAGCCAA GAAAGATCGT GGACACTCGA 
TTTAAGGAGC CTCCAGCCTT TCTGGCTGTT ATTGGAGACC AAAGCGTAAT AGAGGGTCAG 
GAGGTGACCA TGTCTGTTCT AGTGACCGGA CAACCTAAAC CTATGCTTTA CTGGCTGAGA 
GACAGAGTGA CCATTAAAAC CGGAACACGG CATATCGTCC AAGAGACAGA GGAGGGCAAC 
TTTGAAATGA TAATTAAATC AGCACAGAGG TCAGACACCG GTGTTTACAC CTGCAAGATC 
ATCAATGAAT ATGGAACTAA GCAATGTGAA GGCAAACTGG AGGTGAAAGC TCGTCCAGTA 
GAGCCAGGTC TGGCCATCAT TCGGCCAGTG AGGGACGTGA TGGTGAAGGC AGGGGAGACC 
GCTCTGTTTG AGTGTCATGT GATCGGCCCG CAGGACACAG ATGTTGACTG GCTGTCAGAT 
GGGAAACTCA TCCAGCCTGC TTTGTTGAAC TGCAAGATGC ATTTTGATGG GAAAAGGTGC 
AGGCTGCTTC TAAACTCTGT ACATGAGGAT GACAGTGGAA CGTACACCTG CAAACTGAGC 
ACAGCTAAAG AGGAACTGAC CTCCAGTGCC AAGCTAAAAG TCATAGCATC CATAGAGCCC 
CTTTTCACTC GTAAGCTGGA TGTGCTGGAA GTTATCGAGG GTCGTAATGC ACGATTTGAC 
TGCAAGGTCA GCGGGACGCC GCCGCCTCAA GTCATATGGA GCCACTTCGA TCGTCCGCTC 
GAGGAAAACG AAGATATCAG GATCTTAAAA GAAGGAGGAC GGCACTCATT AATCATCTCT 
CATGTTAGTA ATGAAGATGA GGGCCTCTAC ACGGTTGCAG CGCGAAACTC ACACGGGGAA 
GACGAATGTG CAGCGGAGCT CTATGTGCAG GAGCCCAGAC CAGCAATATC TTCTCAAATA 
GCGAAGCTGG AAAAAATGCC TTCCATTCCC GAAGAACCCG AAGTTCCTGA AAATGAGATT 
GAGCGTTTCA CCATGCCTGA CTTCGTAAAG CCGCTTTATG ATATGGATGT AGTGGAGGGC 
AGAGAGGCTG TGCTCAGGTG CAAAGTGGCC GGCTTGCCGT ACCCCACCAT AACATGGTTT 
CACAATGGCA AGAGAATTGA CAGCACGGAG GACAGAAAGA TGACACAGTT CAGAGATGTT 
CACAGCCTGG TGGTCCGCTG TGTTTGTCAT GCTCACGGAG GGGTTTACAA GTGTGTCATA 
TCCAATAAAG TGGGCAAGGC CACCTGCTAT TCTCATCTTT ACGTGACAGA CATCCTTCCT 
GACCCTCCAG ATGGTGCCCC AGTCGTTGAG TCCATCACTG GCAAGACTAT CACGCTAAGC 
TGGAAGAAAC CCAAAAGACT GGATCCGTCC ATTGACCCCA GCTCCCTGAT GTATGCCATT 
CAGCAACAGG CCCTGGGGTC GATCCAGTGG ACCATCATCG CTTCTTGCCT GAAACAGACC 
ACCTATACCA TCAGCAATCT GTCTAAAGGT GTCCGCTACG CCTTCAGGGT GCTATCAATC 
ACCAGCAAGG CCTTTAGCAA GCCGTCGCCT GCCACCGAGC CAATGCAGCT GCTAGATAGA 
GGTCCGTACG TCCAGGAGGC TCCGGTAATC ATTGACAAGC CAGATATCGT GTATATGGTG 
GAGAAGCAGC CCTTGAGCAT CACAGTCACT CTCAACCACG TCAACGCATC GGTCACATGG 
AAGAGAGGAG GAGTGACATT ATCTAACCGA CCGGGTCTTT TTGAGTTGAG CATGCCTGAT 
GATGACCAGC ACACACTGAA GCTGTGTAAG GTGAAGAGCA GTGATGTTGG CCAGCTCACC 
TGCATTGCCA ACAACAAGTA CGGCAGCGAC TCCTGTGTCC TCACCCTGGA GATGGCAGTG 
GCACCAACTT TTGAGACAAT TATGGAAGAC CTGGATGTGA ATGTAGGCGA GACTCCTCGA 
TTTGCAGTGG TGGTTGAAGG AAAGCCCGTC CCAGACATCC TCTGGTACAA GGGAGACACC 
CTTCTGTCCG AAAGCAGCCA TTTCACATTT GTGTATGATG ACAATGAATG TTCTCTAGTG 
GTGCTCAACA CTCAAGCAGA CGACTCAGGA GTGTACACTT GTACTGCGAA GAATCTGGCC 
GGTTCTGTGT CCTGCAAGGC TGAGCTGACC GTCCACACCA CCAAACAGTA CAAAGACGAC 
CCGATGGAGG ATGAGGCGTC CATCCTCAGA AAGATGCGGA GGCTCACTGA TTACTATGAT 
GTGCACAAGG AAATTGGGAG AGGGGCTTTC TCATATGTGA AGCGGGTGAT ACAGAAGGCA 
GGAAAGCTGG AATACGCAGC CAAGTTTATT TCTGCTCGGG CTAAACGAAA GGCATCCGCT 
CTGAGAGAAC TGAACATCCT CTCTCATCTG GACCATGAGA GGATCCTCTA TTTTCATGAT 
GCCTTTGAGA AGAAGAATGC TGTCATCATC ATTACAGAGC TATGCCATGA GGAGCTTCTC 
GATAGGCTTA CAAAGAAATC AACCATCTTG GAGTCCGAGA TCCGATCCAG CGTGAGGCAG 
CTGTTGGAGG GTATTAACTA TCTCCATCAG CTTGACATAC TTCATCTAGA CATCAAGCCT 
GATAATATCC TCATGGCAGA CCACAGTAGC GATCAGATCC GCATATGTGA TTTTGGAAAT 
GCTGTAAAGT TCATGCCTGA TGAGGCACAG TACTGCAAAT ATGGCACTCC AGAATTTGTC 
GCCCCAGAGA TTGTTAACCA GACACCCGTC TCCAAGGCAA CGGACATCTG GCCCATTGGT 
GTTCTGACAT ACTTATGTCT TACTGGAGTT TCTCCTTTTG CTGGGGAAAA CGACCGCAGC 
TCTGTTCTCA ACATCAGGAA TTATAATGTG GCTTTTGAAG AAAGCATGTT TACAGACCTC 
TGCCATGAAG CCAAGGGATT CGTCATCAAG CTGCTGGTGG CTGATAGATT GAGGCCTGAT 
GCTAATGAAT GTCTTCGCCA CCCCTGGTTC AAGACCCTCA ACAAGGGTAA AAGCATCAGT 
ACAGAGTCCC TTAAAAAGTT TTTGTCCAGA AGAAAATGGC AGCGATCGCT TATCAGCTAC 
AAATCTAAAA TGGTGATGAG GTCTGTACCT GAGCTACTGG ATGACTCCTC CAGCCATATT 
TCCATTGCTG TTCCGCGACA TCTGAAAAAA GGCTCACCAC CACTTTCATC ATCTTCAGAT 
TCAGATGAAG ACATCGATGA GTTGCCTTTT ATTCCCATGC CACTCAACGT GGAATTCTCA 
GGATCTAGAA TGTCACTGAC CGAAATTGTA GGTGATGATG ACATGACAGG TAAATTGAAT 
GGAAATGCTG AGAGGCCTGC TTCACTCCAT CCGAAGTCCG AGCCAATGGA AGTGGAGATG 
TCAGAGAGAG AAGGTGGAGA TCCATCTGCT AGGGGTAGAG CCAAGAAGAG GACTACAACA 
GAAGATGAAA AAGGGTCGTC TGATGAGGAA CCATCACAAC TGGCCAAAAG AGCAGAACTG 
GCAAAGAGAC CAATGCGCAG AGGTTCAAGC ATGGAGTCAG ATGAGCCAGA GGGAGGACGT 
CGCAGAGGAG AGTTACGCAG AGGGAGTTCG GCTGACAGTG CCTTACAGCT CCATATCAAA 
CCAGAGGAGG GCACAGAAGA AAGTCCTACT GATGGAAGTA GAATTCTACA AAAGTCTGTA 
TCTATGGAGC TTCCAAGGAG AAGCCCAAGT CCAGGTGCTG CAAAGCTTAG CCAGGAAGAC 
TATGCTCTGA AACTGGAACT GATGAGGCAG CGACTACTCA GGGGTGGCTC TGCTGACAAC 
AAGATGAGTG GATTACGAGG CCCATTGCTG GAGACACTAG GTATGGGAGA TGAGAAGCGG 
TTTGTGCGGG GATCTCGTCT AGGTAACCCT CCACTTGTTA GAGCGGCATC TAGTGAGTCA 
CCTAGAGATG ACATCCCAAA GACAAAGATT CTCCGCAAGA GTGCTTCCTT TAGCCAGGGG 
GATTCAGAGC CCATGCCCCT GCACAGAAGA ATCGGAGCAC CTCTAGAGAT TCCTCTAGCA 
CAAATAGAGG AGAGACGCCT ACAGGAAGCA ATATCCATGT CTGCTCTCAC AGAGCCTGTC 
AAAGATTCTC GCCCAATCAC TCCAAAACTT CAAATGCCTG AAGAAGAAAG ACATGAAAAG 
AAGAAAACTG AAGAAAAAGA ATCTGTCTAC AAGGACAATA AACCTGAAGA GGTTATTGCT 
GTGTCAAAGA CTGAGGAAGA AGGAAGACGT GTTGATCAGA AATCTTGGGA TTCTGATGCA 
CAGAAAAAGG ACCAGAGCAA GTCAGAGGAA ACCAGGGAAG AGTCTGAAAA AGTGGCTGAA 
ATTGAAAAAA AGACCTCTAT AGTGGACGAA ACAATTGAGG AAGAGGAAAC AAGAAAAGAT 
GAGGAGCCAG AGAAAGAAAC TGAGCCTCCT ACTCCAAAGA TTGAGGTTAA GGAATATGAG 
GGTGATGTAA AGGTATTACC AAAGTCTCCA GAGTTTATCA TTTCCACAAA CTCAATCATG 
GTGACCCCAA CAGCTGCCCC AGCATCCAGT CCCCAGAGTG TATCAACCTA TGTTACCCCA 
TCTCCCCCTG CACGCACAGT GCTCCCAGAT GGTAGGATGT CCGCATATGC CAGTATTATG 
CAGACTATCA TGGTACCATC TGTCCCAGCA AATACCCAAT CTGACACTGT TCCTATTCCT 
ACACCAACTT CCACTCCACC ATCAGTTCCA GTCTATGCAC CCATTTCCAG ACACATCTCA 
GAACCAACCC TTTCATCAAC TTCTTCATCC AAATCAGATC TTCACCAAAC AACTGAGCAT 
CCTGCAGTCT TTTCTCGGGT TGCCTCCACT GAACACCCTC CCAAAGAGCC AAGTCCTCCT 
AAGACACCCA CTCGCGCCTC CCCTAAGCGA GAGCCATCTC CTGGAAGCAA CATCCAGGAC 
CTTGCATCCG AGGAGGTTTT TGAAGCTCGC TTCAAGAAAC GTGAGTCTTC TCTAACTCGT 
AGTCTCAAAC GTTTCTCATT TCAGAAGACT GAAGACAAGC CCTCCACTGT TTCTCCTGAA 
ACTGCTGAAG AAATGTATCG CCCTGGACCA ACTGGTGCTC CTTTAGAGTT TGTGCCAAGG 
AGGTTGGAGG AGAAGTCCAA ATCAGTACAA GATCTTCGAG AGGCAGAGAA GGACCCTGGG 
TTCATGAGGC GACTCTCCAT GCGCTTGAAA AGGACCCCAT CAGTGGAAAA GCAGGAGGAG 
AAGCTCAAAG AGGAGGAGGC TTCAGCACCC AGGCGTCGTC TTTCTTGGGC ACTGGGACGG 
AGGGGATCCC AAGAGAAGAA GGAGATTGAG ATGATGAGGT TGGATGGGGG TCCTGAGCCT 
CCCCCAGAGC CTGAGTCTAA AGCACCTTCT GAGTCACCTA TTCTTGCCAT GCGCAAGAAG 
ATTGGCAGCA CCATGGCAAG CTTATCTACA AGAATTAGGA GCCATTCTGA GGACAGGAAA 
GATGACAGTG AAGGCAAGAT GGAGACCAAG AGGACTCCTA TATTCAACAA ACTAAGGCGT 
TCCACTTCTG AGGGGGGCAG TCTGAAGAGA ATGGGTATCC CACAAAACCA ACTGGCTGCT 
CAGTCAGGGC AAGGGACCTC ATCAGAATCT CTTGATTCTA TGTCAAGCAT CCAGTCAGAA 
TCAGCTATTA AAAATGCAGA ACTTGACCGC AGATCACGGT GGGACCGATG GGGCTTGACT 
CGAGGGAAAA AGGACAAGAC AGTTTCCCAA CCAGACATCC CAGCTTCCAT TGCTAGAGAG 
AATGGTTCTC TACGGTCTCG CCAATATACA CGTCTAACAT CTGACTTCCC TCCGGTGTTC 
CATATCAAAC TGAGGGACCA TGTACTCTTG GAGGGAGATC CTGTCACTCT CAGCTGCCTC 
CCTGCTGGCA GTCCTCACCC ACACATCTCA TGGATGAAAG ACAAGAAGCC ACTTGAGATT 
GACCCCAGGA TGAATATGAT ATCTTGCCCC GATGGGAGAC AACTGCTGAT GATTATGAAG 
ACCACCAAGA AGGATGCAGG CCTCTATGAA TGTGTGGCTG CAAACCCACT GGCCACAGTC 
ACCAGCTCAT GCGTTGTGTC CCTCGCTCGT CTGCCTAATA GTCCTGGGAC ACCCGAGGTT 
CCTCAGAAGT ATAAAAACAC TGCTCTAGTG GTATGGAGAC CCTCAGACAC TACGGCACCC 
TGCACCTACT CTCTGGAGAG GAAGACAGAG GGTGAGAATA ACTGGCTGAT AGTCGCCACA 
GGTGTTGCTG ACTGTTACTA TAACGTGTTG GATCTTCCGG CCGGCGCTTC ATACAGGTTC 
AGAGTGGCCT GCGTCAATAA GGCTGGTCAG GGACCTTACA GCAGCTTGTC TGAAGTAGTG 
GTCCTGGACT CAACAGAGCC CCAGAAGTCC AGTGGCACAG TTGTAGTCAA AACGCTTTCA 
TCAGCTCCCG CTCCTGCAGT GGTAACGTCC ACCATGACCC TTCCTGCAAT GAAGCCTGCT 
GCAATAAAAC CAGCACCATC AACAAATTCA GCGCCCTCTA CAGTCCAACC GGTAAAGCCT 
GCGCCTCCTC CTGCATCATC AACACCTACC TATCGTCCCG TGCCTGACAT TCGAACTCCA 
TCGCCTGGCA CTCCAACCCC TGCCAAAGCC AAAAGCACCC TCAACATTAC TATGGCCAAA 
CCCTCCCCAG CATCCCCTGC AGCTCAACCA CAACCACAAC CTCCACCCAA ACCCTCGCCT 
CCGGTCATTC TGCCCAAACC TCAGACCCCA GTCAGTGTGG TTTCACCTCC ATCCCAAACT 
CCTCCAGTGT CACCTCCACC TCTAGTGTCT CCTCCTCCCA TTATTGGTAA ACCAATCTCC 
TCAGTGCCCA TGTATGTCCC TGCTACAACC ACCTCGACTC CAGTGACTCC GGTCACTCCT 
GTCACCCCAG TCACTCCAGT CACCCCAAAT ACGCCCTCTC CTGCCCTGTC GCCTCCAGTG 
GTGTTGGTTA CAAGTCTGAG TCCTGTAGGG GAGGGAACTG GGACACCCAA TCGAATGACC 
CCTAGTGGAA GAGCCACGCC TTCTGGACGC ATCACTCCAT CAGGCCGAAG GACCCCTATT 
GGAAAGCCTG GAGACTCAAC GCTGAGGCAA GGAGTCCCTC AGAAACCTTA CACCTTCATG 
GATGAGAAAG CAAGGGGTCG CTTTGGTGTG ATCAGAGAGT GTCGAGAAAA TGCCACTGGT 
AACCTGTACA TGGCTAAGAT TGTGCCATAT GAGCCAGAGA GCAAGCAGAC TGTATTACAG 
GAATACGACA TCCTGAAGTC TCTCCATCAT GAGAAGATCA TGGCTCTCCA CGAGGCCTAC 
GTCACTCCAC GCTACCTGGT GCTCATCTCT GAGTGCTGCT CTGGCAAAGA ACTACTCCAC 
AGTCTCATTG ACAGGTTCCG ATACTCTGAG GATGATGTGG TTGCATATAT CGTACAGATC 
CTTCAGGGTC TGGACTACCT GCACAGCCGG AGGATCCTGC ATCTCGACAT CAAACCAGAA 
AACATCATCG TCACTTACAT GAATGTGGTG AAGATTATCG ATTTTGGTAG TGCACAGACC 
TTCAATCCTC TGTTCCTGAA GCAGTTCTCC CCACCTATTG GAACACTGGA TTATATGTCT 
CCAGAGATGC TGAAAGGAGA TGTGGTGGGT CCACCAGCTG ACATCTGGAG CATTGGCATA 
TTGACTTATA TCATGCTTAG CGGGAGGTTG CCGTTCACAG AAAATGACCC TGCCGAGACC 
GAGGCCAGAA TACAGGCAGC CAAGTTTGAC CTCAGTAAGC TTTACCAAAA CGTGTCCCAA 
AGTGCCTCTC TGTTCATCAA AAAGATCCTG TGCAGCTACC CTTGGGCCCG TCCGACCATC 
AAGGACTGCT TTACCAACTC GTGGCTGCAG GACGCATATC TGATGCGCCT GCGAAGACAG 
ACTCTCACAT TCACCACCAC ACGCCTCAAA GAGTTCCTGG AGCAGCAGCA GCACATCAGA 
GCCCAAGAGG CAACAAAGCA CAAAGTCCTC CTGCGTTCCT ACCAGAGCAA CCCACAAACC 
CCATCCACCC CTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spegb ( XM_021479009.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334684.1 Danio rerio SPEG complex locus b (spegb), transcript variant X4, mRNA.

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CloneID	ODa144105
Clone ID Related Accession (Same CDS sequence)	XM_021479009.1
Accession Version	XM_021479009.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10428bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	striated muscle preferentially expressed protein kinase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAA TCTGGTCCAA GAAACGCTTT CAGAAAACCG GACACTCTAC CCGAACATTT 
GGGCGGTTCA CTCATGTTTT CCGCTCCTGT CGGAAACAAA GTTATGACTC TGAGACTACA 
GAAGACGAAG CCGCTGAGCC ACAAATACCC CTGGAGGCCA AAGAAGGAGC CAGAGGTTTT 
CAGGATGAGG CCCAACGAGT GCCTGCAGGT GAGAGAATGA TGGATGTCGC CCCAGATGCG 
AGCAATCGCT GGGCCGCCAG TGAAAGCACA GTGTTGGAGA GAGAAGCTCT AGCCCTGGGA 
TCGAGAGCCC CAGGGCCGCA GTCTGCCAGG AGCCCATCTG ACGATGTTTT GAGGGATTCT 
CCCCCTCAAG TTCTACCACC CCCTTCCCCC AAATTTGGCC GCTCTACTGC CTCTCCTTTG 
CTCAGCCGTT CTGGTTCGGG CCCTGCCACA CCCCTTGCCC CTCGCAAGAA AGTTGTCGTG 
CCAACTGAAT ATCAAGACAC AGTACCAGGA GAGTTTGAGG AGAAGATTAA GCAACCCAAA 
TCCTCTGGGA TGTCTCAGAG TAGTGCTCAA GACTCTCGAC CCCAAACCCC TGTCAGTGAG 
TATTCCCGTA AGGAGCTTAC ACTTCGGCCC TCACCAAAAC TGACAAGAGC TAGCTCCAAA 
ATCTTTGAGA AGGTGCGAGT CTTCGAAGAG CGCAGAAGAA GCATTGATAA CCCTGAAGGA 
TCTATATCTG GACGTTCTTG GGCTGGATTT AACAGAGCTT CATCTATCGA CTCTGATGAA 
GGAGGGAGCA GGTTGGGCAT CTCCAGGGAG AGCTCCAAAG AAGACCTTCG TGAGGCTTTG 
AAGGCAGATG CAGCCCAACG AAGGACCATG TTTAAACAGC GGGCTGCTTC CCTGGAGGAC 
AGACCTCGTT ACACCCAGAA GGTGCAGGAC ATTGAAAACA AGTTCACAGA GGAGCTCCAG 
CGCATCAAAA AGCTTGTCGG CAAACCACAC ATGAAGAAAT CCTTCTCAAC AGAGCAGCTC 
TCTACTTGTC ACAGAAGCAG GCAGCCTGTA AGAAAGTTAG AGCCCATCCC ACCTCAGGTC 
CTTCAAAAGC TGCAGGATAG AGAACGTGCT CAGCTAGCTC AGCTAGAGCA AGAGATGAGA 
GAGCGAGACC AGGCCAAAGA ACGATCACCT CCGAAATCAC CGTCTCGCAG AAGGAAAGAC 
CATCTGGCAC AACAACCACC AGAAAGAGCT GAGACCAAGG TGGTAAGCAC AACTGGACTT 
GAACCATCCC CTCCAGAAGC CATGTCACTC TCTGATTTAC CAGGTCAAAG ATCTCCAAGA 
TTTCGGGGCC CAAGCCCAAG CAGAGATAGT ACACGAAGGT CTCCAACCGT AGAGATTAGT 
GCCATGGCTG AGGAGCGCCC TCGAGGAAGA GCTGAATCTC CTTCAAGAAA TGTTTTGGAG 
ATGACGTTAC GCAAAGTGGA GCCAAGACCT GCAAGTCCAC TGGTAAAGAG GGTGGTTCGT 
GTTCAAGAAG TTCCACAAGC AAACGATCAG CCTATGCATA GAAAGACGCC AATAGAGGTC 
TCGCTAAGAA AACTGGAAAG AAGGCCTGAA AGTCCCCTGG TGCAAGGAGA AACCGTAGCC 
ATTCAAGACT TCCCTGTTCA AGCTCCCCCC AAACCTCCAA GAGTTTCCTC ATCTTCCTCG 
TCTGAGGAGA AGATGGAGCT GGAAGTGACT CCTCCACCAC CCCCACTAAA ACTCACTATA 
CCAAAAATTA TTGTTGAAGA AGAGCCCATG GAGACAGACA CACCTGTTGA GAAGACTGAA 
AAGGCTGTGA AAGTTGGCAC TGGTAAAGAT GAAAAACATC AAAGATCCAG AGGAAGAGGA 
CGCAGACAAA GACCAATGTC CCCTGAATTA GAATCTTCGG ACGACTCCTA TGTGTCTGCA 
GGAGAGGATC CACTAGAAGC TCCTGTGTTC GAGTTTCCAC TTCAGGACAC TGTGGCCTCC 
ACTGGAACGG AGGTGCTATT AAAATGCATC ATCAGTGGCA CCCCTCTCCC TGAAGTTATA 
TGGAAGAAAG ACAACACTGA GGTCAAGAAC AGTCCCACTC ATGTTGTAAA GATAGAGGGG 
GAACGCCACT CCTTGCTCAT CAAATGGACA AAGCCAAGTG ATGCAGGAAC CTACACAGTT 
ACCGCTGTCA ATGAGGTCGG TGAGGTGTCC AGCAGTGCTA CACTTTTCAT CAAACCAGAG 
CCCAGTCAGG ACCATCGTGG TAACCTTGGT GTCCCTATGG ATGTCAGCAG TCCCATCACA 
TCTGATGAGG AGTATCTCAG TCCACTGGAA GAGAGCATGG ACTTCTCCTC CTACAGGGGA 
CCGGAGCCAA GAAAGATCGT GGACACTCGA TTTAAGGAGC CTCCAGCCTT TCTGGCTGTT 
ATTGGAGACC AAAGCGTAAT AGAGGGTCAG GAGGTGACCA TGTCTGTTCT AGTGACCGGA 
CAACCTAAAC CTATGCTTTA CTGGCTGAGA GACAGAGTGA CCATTAAAAC CGGAACACGG 
CATATCGTCC AAGAGACAGA GGAGGGCAAC TTTGAAATGA TAATTAAATC AGCACAGAGG 
TCAGACACCG GTGTTTACAC CTGCAAGATC ATCAATGAAT ATGGAACTAA GCAATGTGAA 
GGCAAACTGG AGGTGAAAGC TCGTCCAGTA GAGCCAGGTC TGGCCATCAT TCGGCCAGTG 
AGGGACGTGA TGGTGAAGGC AGGGGAGACC GCTCTGTTTG AGTGTCATGT GATCGGCCCG 
CAGGACACAG ATGTTGACTG GCTGTCAGAT GGGAAACTCA TCCAGCCTGC TTTGTTGAAC 
TGCAAGATGC ATTTTGATGG GAAAAGGTGC AGGCTGCTTC TAAACTCTGT ACATGAGGAT 
GACAGTGGAA CGTACACCTG CAAACTGAGC ACAGCTAAAG AGGAACTGAC CTCCAGTGCC 
AAGCTAAAAG TCATAGCATC CATAGAGCCC CTTTTCACTC GTAAGCTGGA TGTGCTGGAA 
GTTATCGAGG GTCGTAATGC ACGATTTGAC TGCAAGGTCA GCGGGACGCC GCCGCCTCAA 
GTCATATGGA GCCACTTCGA TCGTCCGCTC GAGGAAAACG AAGATATCAG GATCTTAAAA 
GAAGGAGGAC GGCACTCATT AATCATCTCT CATGTTAGTA ATGAAGATGA GGGCCTCTAC 
ACGGTTGCAG CGCGAAACTC ACACGGGGAA GACGAATGTG CAGCGGAGCT CTATGTGCAG 
GAGCCCAGAC CAGCAATATC TTCTCAAATA GCGAAGCTGG AAAAAATGCC TTCCATTCCC 
GAAGAACCCG AAGTTCCTGA AAATGAGATT GAGCGTTTCA CCATGCCTGA CTTCGTAAAG 
CCGCTTTATG ATATGGATGT AGTGGAGGGC AGAGAGGCTG TGCTCAGGTG CAAAGTGGCC 
GGCTTGCCGT ACCCCACCAT AACATGGTTT CACAATGGCA AGAGAATTGA CAGCACGGAG 
GACAGAAAGA TGACACAGTT CAGAGATGTT CACAGCCTGG TGGTCCGCTG TGTTTGTCAT 
GCTCACGGAG GGGTTTACAA GTGTGTCATA TCCAATAAAG TGGGCAAGGC CACCTGCTAT 
TCTCATCTTT ACGTGACAGA CATCCTTCCT GACCCTCCAG ATGGTGCCCC AGTCGTTGAG 
TCCATCACTG GCAAGACTAT CACGCTAAGC TGGAAGAAAC CCAAAAGACT GGATCCGTCC 
ATTGACCCCA GCTCCCTGAT GTATGCCATT CAGCAACAGG CCCTGGGGTC GATCCAGTGG 
ACCATCATCG CTTCTTGCCT GAAACAGACC ACCTATACCA TCAGCAATCT GTCTAAAGGT 
GTCCGCTACG CCTTCAGGGT GCTATCAATC ACCAGCAAGG CCTTTAGCAA GCCGTCGCCT 
GCCACCGAGC CAATGCAGCT GCTAGATAGA GGTCCGTACG TCCAGGAGGC TCCGGTAATC 
ATTGACAAGC CAGATATCGT GTATATGGTG GAGAAGCAGC CCTTGAGCAT CACAGTCACT 
CTCAACCACG TCAACGCATC GGTCACATGG AAGAGAGGAG GAGTGACATT ATCTAACCGA 
CCGGGTCTTT TTGAGTTGAG CATGCCTGAT GATGACCAGC ACACACTGAA GCTGTGTAAG 
GTGAAGAGCA GTGATGTTGG CCAGCTCACC TGCATTGCCA ACAACAAGTA CGGCAGCGAC 
TCCTGTGTCC TCACCCTGGA GATGGCAGTG GCACCAACTT TTGAGACAAT TATGGAAGAC 
CTGGATGTGA ATGTAGGCGA GACTCCTCGA TTTGCAGTGG TGGTTGAAGG AAAGCCCGTC 
CCAGACATCC TCTGGTACAA GGGAGACACC CTTCTGTCCG AAAGCAGCCA TTTCACATTT 
GTGTATGATG ACAATGAATG TTCTCTAGTG GTGCTCAACA CTCAAGCAGA CGACTCAGGA 
GTGTACACTT GTACTGCGAA GAATCTGGCC GGTTCTGTGT CCTGCAAGGC TGAGCTGACC 
GTCCACACCA CCAAACAGTA CAAAGACGAC CCGATGGAGG ATGAGGCGTC CATCCTCAGA 
AAGATGCGGA GGCTCACTGA TTACTATGAT GTGCACAAGG AAATTGGGAG AGGGGCTTTC 
TCATATGTGA AGCGGGTGAT ACAGAAGGCA GGAAAGCTGG AATACGCAGC CAAGTTTATT 
TCTGCTCGGG CTAAACGAAA GGCATCCGCT CTGAGAGAAC TGAACATCCT CTCTCATCTG 
GACCATGAGA GGATCCTCTA TTTTCATGAT GCCTTTGAGA AGAAGAATGC TGTCATCATC 
ATTACAGAGC TATGCCATGA GGAGCTTCTC GATAGGCTTA CAAAGAAATC AACCATCTTG 
GAGTCCGAGA TCCGATCCAG CGTGAGGCAG CTGTTGGAGG GTATTAACTA TCTCCATCAG 
CTTGACATAC TTCATCTAGA CATCAAGCCT GATAATATCC TCATGGCAGA CCACAGTAGC 
GATCAGATCC GCATATGTGA TTTTGGAAAT GCTGTAAAGT TCATGCCTGA TGAGGCACAG 
TACTGCAAAT ATGGCACTCC AGAATTTGTC GCCCCAGAGA TTGTTAACCA GACACCCGTC 
TCCAAGGCAA CGGACATCTG GCCCATTGGT GTTCTGACAT ACTTATGTCT TACTGGAGTT 
TCTCCTTTTG CTGGGGAAAA CGACCGCAGC TCTGTTCTCA ACATCAGGAA TTATAATGTG 
GCTTTTGAAG AAAGCATGTT TACAGACCTC TGCCATGAAG CCAAGGGATT CGTCATCAAG 
CTGCTGGTGG CTGATAGATT GAGGCCTGAT GCTAATGAAT GTCTTCGCCA CCCCTGGTTC 
AAGACCCTCA ACAAGGGTAA AAGCATCAGT ACAGAGTCCC TTAAAAAGTT TTTGTCCAGA 
AGAAAATGGC AGCGATCGCT TATCAGCTAC AAATCTAAAA TGGTGATGAG GTCTGTACCT 
GAGCTACTGG ATGACTCCTC CAGCCATATT TCCATTGCTG TTCCGCGACA TCTGAAAAAA 
GGCTCACCAC CACTTTCATC ATCTTCAGAT TCAGATGAAG ACATCGATGA GTTGCCTTTT 
ATTCCCATGC CACTCAACGT GGAATTCTCA GGATCTAGAA TGTCACTGAC CGAAATTGTA 
GGTGATGATG ACATGACAGG TAAATTGAAT GGAAATGCTG AGAGGCCTGC TTCACTCCAT 
CCGAAGTCCG AGCCAATGGA AGTGGAGATG TCAGAGAGAG AAGGTGGAGA TCCATCTGCT 
AGGGGTAGAG CCAAGAAGAG GACTACAACA GAAGATGAAA AAGGGTCGTC TGATGAGGAA 
CCATCACAAC TGGCCAAAAG AGCAGAACTG GCAAAGAGAC CAATGCGCAG AGGTTCAAGC 
ATGGAGTCAG ATGAGCCAGA GGGAGGACGT CGCAGAGGAG AGTTACGCAG AGGGAGTTCG 
GCTGACAGTG CCTTACAGCT CCATATCAAA CCAGAGGAGG GCACAGAAGA AAGTCCTACT 
GATGGAAGTA GAATTCTACA AAAGTCTGTA TCTATGGAGC TTCCAAGGAG AAGCCCAAGT 
CCAGGTGCTG CAAAGCTTAG CCAGGAAGAC TATGCTCTGA AACTGGAACT GATGAGGCAG 
CGACTACTCA GGGGTGGCTC TGCTGACAAC AAGATGAGTG GATTACGAGG CCCATTGCTG 
GAGACACTAG GTATGGGAGA TGAGAAGCGG TTTGTGCGGG GATCTCGTCT AGGTAACCCT 
CCACTTGTTA GAGCGGCATC TAGTGAGTCA CCTAGAGATG ACATCCCAAA GACAAAGATT 
CTCCGCAAGA GTGCTTCCTT TAGCCAGGGG GATTCAGAGC CCATGCCCCT GCACAGAAGA 
ATCGGAGCAC CTCTAGAGAT TCCTCTAGCA CAAATAGAGG AGAGACGCCT ACAGGAAGCA 
ATATCCATGT CTGCTCTCAC AGAGCCTGTC AAAGATTCTC GCCCAATCAC TCCAAAACTT 
CAAATGCCTG AAGAAGAAAG ACATGAAAAG AAGAAAACTG AAGAAAAAGA ATCTGTCTAC 
AAGGACAATA AACCTGAAGA GGTTATTGCT GTGTCAAAGA CTGAGGAAGA AGGAAGACGT 
GTTGATCAGA AATCTTGGGA TTCTGATGCA CAGAAAAAGG ACCAGAGCAA GTCAGAGGAA 
ACCAGGGAAG AGTCTGAAAA AGTGGCTGAA ATTGAAAAAA AGACCTCTAT AGTGGACGAA 
ACAATTGAGG AAGAGGAAAC AAGAAAAGAT GAGGAGCCAG AGAAAGAAAC TGAGCCTCCT 
ACTCCAAAGA TTGAGGTTAA GGAATATGAG GGTGATGTAA AGGTATTACC AAAGTCTCCA 
GAGTTTATCA TTTCCACAAA CTCAATCATG GTGACCCCAA CAGCTGCCCC AGCATCCAGT 
CCCCAGAGTG TATCAACCTA TGTTACCCCA TCTCCCCCTG CACGCACAGT GCTCCCAGAT 
GGTAGGATGT CCGCATATGC CAGTATTATG CAGACTATCA TGGTACCATC TGTCCCAGCA 
AATACCCAAT CTGACACTGT TCCTATTCCT ACACCAACTT CCACTCCACC ATCAGTTCCA 
GTCTATGCAC CCATTTCCAG ACACATCTCA GAACCAACCC TTTCATCAAC TTCTTCATCC 
AAATCAGATC TTCACCAAAC AACTGAGCAT CCTGCAGTCT TTTCTCGGGT TGCCTCCACT 
GAACACCCTC CCAAAGAGCC AAGTCCTCCT AAGACACCCA CTCGCGCCTC CCCTAAGCGA 
GAGCCATCTC CTGGAAGCAA CATCCAGGAC CTTGCATCCG AGGAGGTTTT TGAAGCTCGC 
TTCAAGAAAC GTGAGTCTTC TCTAACTCGT AGTCTCAAAC GTTTCTCATT TCAGAAGACT 
GAAGACAAGC CCTCCACTGT TTCTCCTGAA ACTGCTGAAG AAATGTATCG CCCTGGACCA 
ACTGGTGCTC CTTTAGAGTT TGTGCCAAGG AGGTTGGAGG AGAAGTCCAA ATCAGTACAA 
GATCTTCGAG AGGCAGAGAA GGACCCTGGG TTCATGAGGC GACTCTCCAT GCGCTTGAAA 
AGGACCCCAT CAGTGGAAAA GCAGGAGGAG AAGCTCAAAG AGGAGGAGGC TTCAGCACCC 
AGGCGTCGTC TTTCTTGGGC ACTGGGACGG AGGGGATCCC AAGAGAAGAA GGAGATTGAG 
ATGATGAGGT TGGATGGGGG TCCTGAGCCT CCCCCAGAGC CTGAGTCTAA AGCACCTTCT 
GAGTCACCTA TTCTTGCCAT GCGCAAGAAG ATTGGCAGCA CCATGGCAAG CTTATCTACA 
AGAATTAGGA GCCATTCTGA GGACAGGAAA GATGACAGTG AAGGCAAGAT GGAGACCAAG 
AGGACTCCTA TATTCAACAA ACTAAGGCGT TCCACTTCTG AGGGGGGCAG TCTGAAGAGA 
ATGGGTATCC CACAAAACCA ACTGGCTGCT CAGTCAGGGC AAGGGACCTC ATCAGAATCT 
CTTGATTCTA TGTCAAGCAT CCAGTCAGAA TCAGCTATTA AAAATGCAGA ACTTGACCGC 
AGATCACGGT GGGACCGATG GGGCTTGACT CGAGGGAAAA AGGACAAGAC AGTTTCCCAA 
CCAGACATCC CAGCTTCCAT TGCTAGAGAG AATGGTTCTC TACGGTCTCG CCAATATACA 
CGTCTAACAT CTGACTTCCC TCCGGTGTTC CATATCAAAC TGAGGGACCA TGTACTCTTG 
GAGGGAGATC CTGTCACTCT CAGCTGCCTC CCTGCTGGCA GTCCTCACCC ACACATCTCA 
TGGATGAAAG ACAAGAAGCC ACTTGAGATT GACCCCAGGA TGAATATGAT ATCTTGCCCC 
GATGGGAGAC AACTGCTGAT GATTATGAAG ACCACCAAGA AGGATGCAGG CCTCTATGAA 
TGTGTGGCTG CAAACCCACT GGCCACAGTC ACCAGCTCAT GCGTTGTGTC CCTCGCTCGT 
CTGCCTAATA GTCCTGGGAC ACCCGAGGTT CCTCAGAAGT ATAAAAACAC TGCTCTAGTG 
GTATGGAGAC CCTCAGACAC TACGGCACCC TGCACCTACT CTCTGGAGAG GAAGACAGAG 
GGTGAGAATA ACTGGCTGAT AGTCGCCACA GGTGTTGCTG ACTGTTACTA TAACGTGTTG 
GATCTTCCGG CCGGCGCTTC ATACAGGTTC AGAGTGGCCT GCGTCAATAA GGCTGGTCAG 
GGACCTTACA GCAGCTTGTC TGAAGTAGTG GTCCTGGACT CAACAGAGCC CCAGAAGTCC 
AGTGGCACAG TTGTAGTCAA AACGCTTTCA TCAGCTCCCG CTCCTGCAGT GGTAACGTCC 
ACCATGACCC TTCCTGCAAT GAAGCCTGCT GCAATAAAAC CAGCACCATC AACAAATTCA 
GCGCCCTCTA CAGTCCAACC GGTAAAGCCT GCGCCTCCTC CTGCATCATC AACACCTACC 
TATCGTCCCG TGCCTGACAT TCGAACTCCA TCGCCTGGCA CTCCAACCCC TGCCAAAGCC 
AAAAGCACCC TCAACATTAC TATGGCCAAA CCCTCCCCAG CATCCCCTGC AGCTCAACCA 
CAACCACAAC CTCCACCCAA ACCCTCGCCT CCGGTCATTC TGCCCAAACC TCAGACCCCA 
GTCAGTGTGG TTTCACCTCC ATCCCAAACT CCTCCAGTGT CACCTCCACC TCTAGTGTCT 
CCTCCTCCCA TTATTGGTAA ACCAATCTCC TCAGTGCCCA TGTATGTCCC TGCTACAACC 
ACCTCGACTC CAGTGACTCC GGTCACTCCT GTCACCCCAG TCACTCCAGT CACCCCAAAT 
ACGCCCTCTC CTGCCCTGTC GCCTCCAGTG GTGTTGGTTA CAAGTCTGAG TCCTGTAGGG 
GAGGGAACTG GGACACCCAA TCGAATGACC CCTAGTGGAA GAGCCACGCC TTCTGGACGC 
ATCACTCCAT CAGGCCGAAG GACCCCTATT GGAAAGCCTG GAGACTCAAC GCTGAGGCAA 
GGAGTCCCTC AGAAACCTTA CACCTTCATG GATGAGAAAG CAAGGGGTCG CTTTGGTGTG 
ATCAGAGAGT GTCGAGAAAA TGCCACTGGT AACCTGTACA TGGCTAAGAT TGTGCCATAT 
GAGCCAGAGA GCAAGCAGAC TGTATTACAG GAATACGACA TCCTGAAGTC TCTCCATCAT 
GAGAAGATCA TGGCTCTCCA CGAGGCCTAC GTCACTCCAC GCTACCTGGT GCTCATCTCT 
GAGTGCTGCT CTGGCAAAGA ACTACTCCAC AGTCTCATTG ACAGGTTCCG ATACTCTGAG 
GATGATGTGG TTGCATATAT CGTACAGATC CTTCAGGGTC TGGACTACCT GCACAGCCGG 
AGGATCCTGC ATCTCGACAT CAAACCAGAA AACATCATCG TCACTTACAT GAATGTGGTG 
AAGATTATCG ATTTTGGTAG TGCACAGACC TTCAATCCTC TGTTCCTGAA GCAGTTCTCC 
CCACCTATTG GAACACTGGA TTATATGTCT CCAGAGATGC TGAAAGGAGA TGTGGTGGGT 
CCACCAGCTG ACATCTGGAG CATTGGCATA TTGACTTATA TCATGCTTAG CGGGAGGTTG 
CCGTTCACAG AAAATGACCC TGCCGAGACC GAGGCCAGAA TACAGGCAGC CAAGTTTGAC 
CTCAGTAAGC TTTACCAAAA CGTGTCCCAA AGTGCCTCTC TGTTCATCAA AAAGATCCTG 
TGCAGCTACC CTTGGGCCCG TCCGACCATC AAGGACTGCT TTACCAACTC GTGGCTGCAG 
GACGCATATC TGATGCGCCT GCGAAGACAG ACTCTCACAT TCACCACCAC ACGCCTCAAA 
GAGTTCCTGG AGCAGCAGCA GCACATCAGA GCCCAAGAGG CAACAAAGCA CAAAGTCCTC 
CTGCGTTCCT ACCAGAGCAA CCCACAAACC CCATCCACCC CTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334684.1
CDS	940..11367
Translation	MKKIWSKKRFQKTGHSTRTFGRFTHVFRSCRKQSYDSETTEDEAAEPQIPLEAKEGARGFQDEAQRVPAGERMMDVAPDASNRWAASESTVLEREALALGSRAPGPQSARSPSDDVLRDSPPQVLPPPSPKFGRSTASPLLSRSGSGPATPLAPRKKVVVPTEYQDTVPGEFEEKIKQPKSSGMSQSSAQDSRPQTPVSEYSRKELTLRPSPKLTRASSKIFEKVRVFEERRRSIDNPEGSISGRSWAGFNRASSIDSDEGGSRLGISRESSKEDLREALKADAAQRRTMFKQRAASLEDRPRYTQKVQDIENKFTEELQRIKKLVGKPHMKKSFSTEQLSTCHRSRQPVRKLEPIPPQVLQKLQDRERAQLAQLEQEMRERDQAKERSPPKSPSRRRKDHLAQQPPERAETKVVSTTGLEPSPPEAMSLSDLPGQRSPRFRGPSPSRDSTRRSPTVEISAMAEERPRGRAESPSRNVLEMTLRKVEPRPASPLVKRVVRVQEVPQANDQPMHRKTPIEVSLRKLERRPESPLVQGETVAIQDFPVQAPPKPPRVSSSSSSEEKMELEVTPPPPPLKLTIPKIIVEEEPMETDTPVEKTEKAVKVGTGKDEKHQRSRGRGRRQRPMSPELESSDDSYVSAGEDPLEAPVFEFPLQDTVASTGTEVLLKCIISGTPLPEVIWKKDNTEVKNSPTHVVKIEGERHSLLIKWTKPSDAGTYTVTAVNEVGEVSSSATLFIKPEPSQDHRGNLGVPMDVSSPITSDEEYLSPLEESMDFSSYRGPEPRKIVDTRFKEPPAFLAVIGDQSVIEGQEVTMSVLVTGQPKPMLYWLRDRVTIKTGTRHIVQETEEGNFEMIIKSAQRSDTGVYTCKIINEYGTKQCEGKLEVKARPVEPGLAIIRPVRDVMVKAGETALFECHVIGPQDTDVDWLSDGKLIQPALLNCKMHFDGKRCRLLLNSVHEDDSGTYTCKLSTAKEELTSSAKLKVIASIEPLFTRKLDVLEVIEGRNARFDCKVSGTPPPQVIWSHFDRPLEENEDIRILKEGGRHSLIISHVSNEDEGLYTVAARNSHGEDECAAELYVQEPRPAISSQIAKLEKMPSIPEEPEVPENEIERFTMPDFVKPLYDMDVVEGREAVLRCKVAGLPYPTITWFHNGKRIDSTEDRKMTQFRDVHSLVVRCVCHAHGGVYKCVISNKVGKATCYSHLYVTDILPDPPDGAPVVESITGKTITLSWKKPKRLDPSIDPSSLMYAIQQQALGSIQWTIIASCLKQTTYTISNLSKGVRYAFRVLSITSKAFSKPSPATEPMQLLDRGPYVQEAPVIIDKPDIVYMVEKQPLSITVTLNHVNASVTWKRGGVTLSNRPGLFELSMPDDDQHTLKLCKVKSSDVGQLTCIANNKYGSDSCVLTLEMAVAPTFETIMEDLDVNVGETPRFAVVVEGKPVPDILWYKGDTLLSESSHFTFVYDDNECSLVVLNTQADDSGVYTCTAKNLAGSVSCKAELTVHTTKQYKDDPMEDEASILRKMRRLTDYYDVHKEIGRGAFSYVKRVIQKAGKLEYAAKFISARAKRKASALRELNILSHLDHERILYFHDAFEKKNAVIIITELCHEELLDRLTKKSTILESEIRSSVRQLLEGINYLHQLDILHLDIKPDNILMADHSSDQIRICDFGNAVKFMPDEAQYCKYGTPEFVAPEIVNQTPVSKATDIWPIGVLTYLCLTGVSPFAGENDRSSVLNIRNYNVAFEESMFTDLCHEAKGFVIKLLVADRLRPDANECLRHPWFKTLNKGKSISTESLKKFLSRRKWQRSLISYKSKMVMRSVPELLDDSSSHISIAVPRHLKKGSPPLSSSSDSDEDIDELPFIPMPLNVEFSGSRMSLTEIVGDDDMTGKLNGNAERPASLHPKSEPMEVEMSEREGGDPSARGRAKKRTTTEDEKGSSDEEPSQLAKRAELAKRPMRRGSSMESDEPEGGRRRGELRRGSSADSALQLHIKPEEGTEESPTDGSRILQKSVSMELPRRSPSPGAAKLSQEDYALKLELMRQRLLRGGSADNKMSGLRGPLLETLGMGDEKRFVRGSRLGNPPLVRAASSESPRDDIPKTKILRKSASFSQGDSEPMPLHRRIGAPLEIPLAQIEERRLQEAISMSALTEPVKDSRPITPKLQMPEEERHEKKKTEEKESVYKDNKPEEVIAVSKTEEEGRRVDQKSWDSDAQKKDQSKSEETREESEKVAEIEKKTSIVDETIEEEETRKDEEPEKETEPPTPKIEVKEYEGDVKVLPKSPEFIISTNSIMVTPTAAPASSPQSVSTYVTPSPPARTVLPDGRMSAYASIMQTIMVPSVPANTQSDTVPIPTPTSTPPSVPVYAPISRHISEPTLSSTSSSKSDLHQTTEHPAVFSRVASTEHPPKEPSPPKTPTRASPKREPSPGSNIQDLASEEVFEARFKKRESSLTRSLKRFSFQKTEDKPSTVSPETAEEMYRPGPTGAPLEFVPRRLEEKSKSVQDLREAEKDPGFMRRLSMRLKRTPSVEKQEEKLKEEEASAPRRRLSWALGRRGSQEKKEIEMMRLDGGPEPPPEPESKAPSESPILAMRKKIGSTMASLSTRIRSHSEDRKDDSEGKMETKRTPIFNKLRRSTSEGGSLKRMGIPQNQLAAQSGQGTSSESLDSMSSIQSESAIKNAELDRRSRWDRWGLTRGKKDKTVSQPDIPASIARENGSLRSRQYTRLTSDFPPVFHIKLRDHVLLEGDPVTLSCLPAGSPHPHISWMKDKKPLEIDPRMNMISCPDGRQLLMIMKTTKKDAGLYECVAANPLATVTSSCVVSLARLPNSPGTPEVPQKYKNTALVVWRPSDTTAPCTYSLERKTEGENNWLIVATGVADCYYNVLDLPAGASYRFRVACVNKAGQGPYSSLSEVVVLDSTEPQKSSGTVVVKTLSSAPAPAVVTSTMTLPAMKPAAIKPAPSTNSAPSTVQPVKPAPPPASSTPTYRPVPDIRTPSPGTPTPAKAKSTLNITMAKPSPASPAAQPQPQPPPKPSPPVILPKPQTPVSVVSPPSQTPPVSPPPLVSPPPIIGKPISSVPMYVPATTTSTPVTPVTPVTPVTPVTPNTPSPALSPPVVLVTSLSPVGEGTGTPNRMTPSGRATPSGRITPSGRRTPIGKPGDSTLRQGVPQKPYTFMDEKARGRFGVIRECRENATGNLYMAKIVPYEPESKQTVLQEYDILKSLHHEKIMALHEAYVTPRYLVLISECCSGKELLHSLIDRFRYSEDDVVAYIVQILQGLDYLHSRRILHLDIKPENIIVTYMNVVKIIDFGSAQTFNPLFLKQFSPPIGTLDYMSPEMLKGDVVGPPADIWSIGILTYIMLSGRLPFTENDPAETEARIQAAKFDLSKLYQNVSQSASLFIKKILCSYPWARPTIKDCFTNSWLQDAYLMRLRRQTLTFTTTRLKEFLEQQQHIRAQEATKHKVLLRSYQSNPQTPSTPQ

Target ORF information:

RefSeq Version	XM_021479009.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SPEG complex locus b (spegb), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479009.1

ORF Insert Sequence:

ATGAAGAAAA TCTGGTCCAA GAAACGCTTT CAGAAAACCG GACACTCTAC CCGAACATTT 
GGGCGGTTCA CTCATGTTTT CCGCTCCTGT CGGAAACAAA GTTATGACTC TGAGACTACA 
GAAGACGAAG CCGCTGAGCC ACAAATACCC CTGGAGGCCA AAGAAGGAGC CAGAGGTTTT 
CAGGATGAGG CCCAACGAGT GCCTGCAGGT GAGAGAATGA TGGATGTCGC CCCAGATGCG 
AGCAATCGCT GGGCCGCCAG TGAAAGCACA GTGTTGGAGA GAGAAGCTCT AGCCCTGGGA 
TCGAGAGCCC CAGGGCCGCA GTCTGCCAGG AGCCCATCTG ACGATGTTTT GAGGGATTCT 
CCCCCTCAAG TTCTACCACC CCCTTCCCCC AAATTTGGCC GCTCTACTGC CTCTCCTTTG 
CTCAGCCGTT CTGGTTCGGG CCCTGCCACA CCCCTTGCCC CTCGCAAGAA AGTTGTCGTG 
CCAACTGAAT ATCAAGACAC AGTACCAGGA GAGTTTGAGG AGAAGATTAA GCAACCCAAA 
TCCTCTGGGA TGTCTCAGAG TAGTGCTCAA GACTCTCGAC CCCAAACCCC TGTCAGTGAG 
TATTCCCGTA AGGAGCTTAC ACTTCGGCCC TCACCAAAAC TGACAAGAGC TAGCTCCAAA 
ATCTTTGAGA AGGTGCGAGT CTTCGAAGAG CGCAGAAGAA GCATTGATAA CCCTGAAGGA 
TCTATATCTG GACGTTCTTG GGCTGGATTT AACAGAGCTT CATCTATCGA CTCTGATGAA 
GGAGGGAGCA GGTTGGGCAT CTCCAGGGAG AGCTCCAAAG AAGACCTTCG TGAGGCTTTG 
AAGGCAGATG CAGCCCAACG AAGGACCATG TTTAAACAGC GGGCTGCTTC CCTGGAGGAC 
AGACCTCGTT ACACCCAGAA GGTGCAGGAC ATTGAAAACA AGTTCACAGA GGAGCTCCAG 
CGCATCAAAA AGCTTGTCGG CAAACCACAC ATGAAGAAAT CCTTCTCAAC AGAGCAGCTC 
TCTACTTGTC ACAGAAGCAG GCAGCCTGTA AGAAAGTTAG AGCCCATCCC ACCTCAGGTC 
CTTCAAAAGC TGCAGGATAG AGAACGTGCT CAGCTAGCTC AGCTAGAGCA AGAGATGAGA 
GAGCGAGACC AGGCCAAAGA ACGATCACCT CCGAAATCAC CGTCTCGCAG AAGGAAAGAC 
CATCTGGCAC AACAACCACC AGAAAGAGCT GAGACCAAGG TGGTAAGCAC AACTGGACTT 
GAACCATCCC CTCCAGAAGC CATGTCACTC TCTGATTTAC CAGGTCAAAG ATCTCCAAGA 
TTTCGGGGCC CAAGCCCAAG CAGAGATAGT ACACGAAGGT CTCCAACCGT AGAGATTAGT 
GCCATGGCTG AGGAGCGCCC TCGAGGAAGA GCTGAATCTC CTTCAAGAAA TGTTTTGGAG 
ATGACGTTAC GCAAAGTGGA GCCAAGACCT GCAAGTCCAC TGGTAAAGAG GGTGGTTCGT 
GTTCAAGAAG TTCCACAAGC AAACGATCAG CCTATGCATA GAAAGACGCC AATAGAGGTC 
TCGCTAAGAA AACTGGAAAG AAGGCCTGAA AGTCCCCTGG TGCAAGGAGA AACCGTAGCC 
ATTCAAGACT TCCCTGTTCA AGCTCCCCCC AAACCTCCAA GAGTTTCCTC ATCTTCCTCG 
TCTGAGGAGA AGATGGAGCT GGAAGTGACT CCTCCACCAC CCCCACTAAA ACTCACTATA 
CCAAAAATTA TTGTTGAAGA AGAGCCCATG GAGACAGACA CACCTGTTGA GAAGACTGAA 
AAGGCTGTGA AAGTTGGCAC TGGTAAAGAT GAAAAACATC AAAGATCCAG AGGAAGAGGA 
CGCAGACAAA GACCAATGTC CCCTGAATTA GAATCTTCGG ACGACTCCTA TGTGTCTGCA 
GGAGAGGATC CACTAGAAGC TCCTGTGTTC GAGTTTCCAC TTCAGGACAC TGTGGCCTCC 
ACTGGAACGG AGGTGCTATT AAAATGCATC ATCAGTGGCA CCCCTCTCCC TGAAGTTATA 
TGGAAGAAAG ACAACACTGA GGTCAAGAAC AGTCCCACTC ATGTTGTAAA GATAGAGGGG 
GAACGCCACT CCTTGCTCAT CAAATGGACA AAGCCAAGTG ATGCAGGAAC CTACACAGTT 
ACCGCTGTCA ATGAGGTCGG TGAGGTGTCC AGCAGTGCTA CACTTTTCAT CAAACCAGAG 
CCCAGTCAGG ACCATCGTGG TAACCTTGGT GTCCCTATGG ATGTCAGCAG TCCCATCACA 
TCTGATGAGG AGTATCTCAG TCCACTGGAA GAGAGCATGG ACTTCTCCTC CTACAGGGGA 
CCGGAGCCAA GAAAGATCGT GGACACTCGA TTTAAGGAGC CTCCAGCCTT TCTGGCTGTT 
ATTGGAGACC AAAGCGTAAT AGAGGGTCAG GAGGTGACCA TGTCTGTTCT AGTGACCGGA 
CAACCTAAAC CTATGCTTTA CTGGCTGAGA GACAGAGTGA CCATTAAAAC CGGAACACGG 
CATATCGTCC AAGAGACAGA GGAGGGCAAC TTTGAAATGA TAATTAAATC AGCACAGAGG 
TCAGACACCG GTGTTTACAC CTGCAAGATC ATCAATGAAT ATGGAACTAA GCAATGTGAA 
GGCAAACTGG AGGTGAAAGC TCGTCCAGTA GAGCCAGGTC TGGCCATCAT TCGGCCAGTG 
AGGGACGTGA TGGTGAAGGC AGGGGAGACC GCTCTGTTTG AGTGTCATGT GATCGGCCCG 
CAGGACACAG ATGTTGACTG GCTGTCAGAT GGGAAACTCA TCCAGCCTGC TTTGTTGAAC 
TGCAAGATGC ATTTTGATGG GAAAAGGTGC AGGCTGCTTC TAAACTCTGT ACATGAGGAT 
GACAGTGGAA CGTACACCTG CAAACTGAGC ACAGCTAAAG AGGAACTGAC CTCCAGTGCC 
AAGCTAAAAG TCATAGCATC CATAGAGCCC CTTTTCACTC GTAAGCTGGA TGTGCTGGAA 
GTTATCGAGG GTCGTAATGC ACGATTTGAC TGCAAGGTCA GCGGGACGCC GCCGCCTCAA 
GTCATATGGA GCCACTTCGA TCGTCCGCTC GAGGAAAACG AAGATATCAG GATCTTAAAA 
GAAGGAGGAC GGCACTCATT AATCATCTCT CATGTTAGTA ATGAAGATGA GGGCCTCTAC 
ACGGTTGCAG CGCGAAACTC ACACGGGGAA GACGAATGTG CAGCGGAGCT CTATGTGCAG 
GAGCCCAGAC CAGCAATATC TTCTCAAATA GCGAAGCTGG AAAAAATGCC TTCCATTCCC 
GAAGAACCCG AAGTTCCTGA AAATGAGATT GAGCGTTTCA CCATGCCTGA CTTCGTAAAG 
CCGCTTTATG ATATGGATGT AGTGGAGGGC AGAGAGGCTG TGCTCAGGTG CAAAGTGGCC 
GGCTTGCCGT ACCCCACCAT AACATGGTTT CACAATGGCA AGAGAATTGA CAGCACGGAG 
GACAGAAAGA TGACACAGTT CAGAGATGTT CACAGCCTGG TGGTCCGCTG TGTTTGTCAT 
GCTCACGGAG GGGTTTACAA GTGTGTCATA TCCAATAAAG TGGGCAAGGC CACCTGCTAT 
TCTCATCTTT ACGTGACAGA CATCCTTCCT GACCCTCCAG ATGGTGCCCC AGTCGTTGAG 
TCCATCACTG GCAAGACTAT CACGCTAAGC TGGAAGAAAC CCAAAAGACT GGATCCGTCC 
ATTGACCCCA GCTCCCTGAT GTATGCCATT CAGCAACAGG CCCTGGGGTC GATCCAGTGG 
ACCATCATCG CTTCTTGCCT GAAACAGACC ACCTATACCA TCAGCAATCT GTCTAAAGGT 
GTCCGCTACG CCTTCAGGGT GCTATCAATC ACCAGCAAGG CCTTTAGCAA GCCGTCGCCT 
GCCACCGAGC CAATGCAGCT GCTAGATAGA GGTCCGTACG TCCAGGAGGC TCCGGTAATC 
ATTGACAAGC CAGATATCGT GTATATGGTG GAGAAGCAGC CCTTGAGCAT CACAGTCACT 
CTCAACCACG TCAACGCATC GGTCACATGG AAGAGAGGAG GAGTGACATT ATCTAACCGA 
CCGGGTCTTT TTGAGTTGAG CATGCCTGAT GATGACCAGC ACACACTGAA GCTGTGTAAG 
GTGAAGAGCA GTGATGTTGG CCAGCTCACC TGCATTGCCA ACAACAAGTA CGGCAGCGAC 
TCCTGTGTCC TCACCCTGGA GATGGCAGTG GCACCAACTT TTGAGACAAT TATGGAAGAC 
CTGGATGTGA ATGTAGGCGA GACTCCTCGA TTTGCAGTGG TGGTTGAAGG AAAGCCCGTC 
CCAGACATCC TCTGGTACAA GGGAGACACC CTTCTGTCCG AAAGCAGCCA TTTCACATTT 
GTGTATGATG ACAATGAATG TTCTCTAGTG GTGCTCAACA CTCAAGCAGA CGACTCAGGA 
GTGTACACTT GTACTGCGAA GAATCTGGCC GGTTCTGTGT CCTGCAAGGC TGAGCTGACC 
GTCCACACCA CCAAACAGTA CAAAGACGAC CCGATGGAGG ATGAGGCGTC CATCCTCAGA 
AAGATGCGGA GGCTCACTGA TTACTATGAT GTGCACAAGG AAATTGGGAG AGGGGCTTTC 
TCATATGTGA AGCGGGTGAT ACAGAAGGCA GGAAAGCTGG AATACGCAGC CAAGTTTATT 
TCTGCTCGGG CTAAACGAAA GGCATCCGCT CTGAGAGAAC TGAACATCCT CTCTCATCTG 
GACCATGAGA GGATCCTCTA TTTTCATGAT GCCTTTGAGA AGAAGAATGC TGTCATCATC 
ATTACAGAGC TATGCCATGA GGAGCTTCTC GATAGGCTTA CAAAGAAATC AACCATCTTG 
GAGTCCGAGA TCCGATCCAG CGTGAGGCAG CTGTTGGAGG GTATTAACTA TCTCCATCAG 
CTTGACATAC TTCATCTAGA CATCAAGCCT GATAATATCC TCATGGCAGA CCACAGTAGC 
GATCAGATCC GCATATGTGA TTTTGGAAAT GCTGTAAAGT TCATGCCTGA TGAGGCACAG 
TACTGCAAAT ATGGCACTCC AGAATTTGTC GCCCCAGAGA TTGTTAACCA GACACCCGTC 
TCCAAGGCAA CGGACATCTG GCCCATTGGT GTTCTGACAT ACTTATGTCT TACTGGAGTT 
TCTCCTTTTG CTGGGGAAAA CGACCGCAGC TCTGTTCTCA ACATCAGGAA TTATAATGTG 
GCTTTTGAAG AAAGCATGTT TACAGACCTC TGCCATGAAG CCAAGGGATT CGTCATCAAG 
CTGCTGGTGG CTGATAGATT GAGGCCTGAT GCTAATGAAT GTCTTCGCCA CCCCTGGTTC 
AAGACCCTCA ACAAGGGTAA AAGCATCAGT ACAGAGTCCC TTAAAAAGTT TTTGTCCAGA 
AGAAAATGGC AGCGATCGCT TATCAGCTAC AAATCTAAAA TGGTGATGAG GTCTGTACCT 
GAGCTACTGG ATGACTCCTC CAGCCATATT TCCATTGCTG TTCCGCGACA TCTGAAAAAA 
GGCTCACCAC CACTTTCATC ATCTTCAGAT TCAGATGAAG ACATCGATGA GTTGCCTTTT 
ATTCCCATGC CACTCAACGT GGAATTCTCA GGATCTAGAA TGTCACTGAC CGAAATTGTA 
GGTGATGATG ACATGACAGG TAAATTGAAT GGAAATGCTG AGAGGCCTGC TTCACTCCAT 
CCGAAGTCCG AGCCAATGGA AGTGGAGATG TCAGAGAGAG AAGGTGGAGA TCCATCTGCT 
AGGGGTAGAG CCAAGAAGAG GACTACAACA GAAGATGAAA AAGGGTCGTC TGATGAGGAA 
CCATCACAAC TGGCCAAAAG AGCAGAACTG GCAAAGAGAC CAATGCGCAG AGGTTCAAGC 
ATGGAGTCAG ATGAGCCAGA GGGAGGACGT CGCAGAGGAG AGTTACGCAG AGGGAGTTCG 
GCTGACAGTG CCTTACAGCT CCATATCAAA CCAGAGGAGG GCACAGAAGA AAGTCCTACT 
GATGGAAGTA GAATTCTACA AAAGTCTGTA TCTATGGAGC TTCCAAGGAG AAGCCCAAGT 
CCAGGTGCTG CAAAGCTTAG CCAGGAAGAC TATGCTCTGA AACTGGAACT GATGAGGCAG 
CGACTACTCA GGGGTGGCTC TGCTGACAAC AAGATGAGTG GATTACGAGG CCCATTGCTG 
GAGACACTAG GTATGGGAGA TGAGAAGCGG TTTGTGCGGG GATCTCGTCT AGGTAACCCT 
CCACTTGTTA GAGCGGCATC TAGTGAGTCA CCTAGAGATG ACATCCCAAA GACAAAGATT 
CTCCGCAAGA GTGCTTCCTT TAGCCAGGGG GATTCAGAGC CCATGCCCCT GCACAGAAGA 
ATCGGAGCAC CTCTAGAGAT TCCTCTAGCA CAAATAGAGG AGAGACGCCT ACAGGAAGCA 
ATATCCATGT CTGCTCTCAC AGAGCCTGTC AAAGATTCTC GCCCAATCAC TCCAAAACTT 
CAAATGCCTG AAGAAGAAAG ACATGAAAAG AAGAAAACTG AAGAAAAAGA ATCTGTCTAC 
AAGGACAATA AACCTGAAGA GGTTATTGCT GTGTCAAAGA CTGAGGAAGA AGGAAGACGT 
GTTGATCAGA AATCTTGGGA TTCTGATGCA CAGAAAAAGG ACCAGAGCAA GTCAGAGGAA 
ACCAGGGAAG AGTCTGAAAA AGTGGCTGAA ATTGAAAAAA AGACCTCTAT AGTGGACGAA 
ACAATTGAGG AAGAGGAAAC AAGAAAAGAT GAGGAGCCAG AGAAAGAAAC TGAGCCTCCT 
ACTCCAAAGA TTGAGGTTAA GGAATATGAG GGTGATGTAA AGGTATTACC AAAGTCTCCA 
GAGTTTATCA TTTCCACAAA CTCAATCATG GTGACCCCAA CAGCTGCCCC AGCATCCAGT 
CCCCAGAGTG TATCAACCTA TGTTACCCCA TCTCCCCCTG CACGCACAGT GCTCCCAGAT 
GGTAGGATGT CCGCATATGC CAGTATTATG CAGACTATCA TGGTACCATC TGTCCCAGCA 
AATACCCAAT CTGACACTGT TCCTATTCCT ACACCAACTT CCACTCCACC ATCAGTTCCA 
GTCTATGCAC CCATTTCCAG ACACATCTCA GAACCAACCC TTTCATCAAC TTCTTCATCC 
AAATCAGATC TTCACCAAAC AACTGAGCAT CCTGCAGTCT TTTCTCGGGT TGCCTCCACT 
GAACACCCTC CCAAAGAGCC AAGTCCTCCT AAGACACCCA CTCGCGCCTC CCCTAAGCGA 
GAGCCATCTC CTGGAAGCAA CATCCAGGAC CTTGCATCCG AGGAGGTTTT TGAAGCTCGC 
TTCAAGAAAC GTGAGTCTTC TCTAACTCGT AGTCTCAAAC GTTTCTCATT TCAGAAGACT 
GAAGACAAGC CCTCCACTGT TTCTCCTGAA ACTGCTGAAG AAATGTATCG CCCTGGACCA 
ACTGGTGCTC CTTTAGAGTT TGTGCCAAGG AGGTTGGAGG AGAAGTCCAA ATCAGTACAA 
GATCTTCGAG AGGCAGAGAA GGACCCTGGG TTCATGAGGC GACTCTCCAT GCGCTTGAAA 
AGGACCCCAT CAGTGGAAAA GCAGGAGGAG AAGCTCAAAG AGGAGGAGGC TTCAGCACCC 
AGGCGTCGTC TTTCTTGGGC ACTGGGACGG AGGGGATCCC AAGAGAAGAA GGAGATTGAG 
ATGATGAGGT TGGATGGGGG TCCTGAGCCT CCCCCAGAGC CTGAGTCTAA AGCACCTTCT 
GAGTCACCTA TTCTTGCCAT GCGCAAGAAG ATTGGCAGCA CCATGGCAAG CTTATCTACA 
AGAATTAGGA GCCATTCTGA GGACAGGAAA GATGACAGTG AAGGCAAGAT GGAGACCAAG 
AGGACTCCTA TATTCAACAA ACTAAGGCGT TCCACTTCTG AGGGGGGCAG TCTGAAGAGA 
ATGGGTATCC CACAAAACCA ACTGGCTGCT CAGTCAGGGC AAGGGACCTC ATCAGAATCT 
CTTGATTCTA TGTCAAGCAT CCAGTCAGAA TCAGCTATTA AAAATGCAGA ACTTGACCGC 
AGATCACGGT GGGACCGATG GGGCTTGACT CGAGGGAAAA AGGACAAGAC AGTTTCCCAA 
CCAGACATCC CAGCTTCCAT TGCTAGAGAG AATGGTTCTC TACGGTCTCG CCAATATACA 
CGTCTAACAT CTGACTTCCC TCCGGTGTTC CATATCAAAC TGAGGGACCA TGTACTCTTG 
GAGGGAGATC CTGTCACTCT CAGCTGCCTC CCTGCTGGCA GTCCTCACCC ACACATCTCA 
TGGATGAAAG ACAAGAAGCC ACTTGAGATT GACCCCAGGA TGAATATGAT ATCTTGCCCC 
GATGGGAGAC AACTGCTGAT GATTATGAAG ACCACCAAGA AGGATGCAGG CCTCTATGAA 
TGTGTGGCTG CAAACCCACT GGCCACAGTC ACCAGCTCAT GCGTTGTGTC CCTCGCTCGT 
CTGCCTAATA GTCCTGGGAC ACCCGAGGTT CCTCAGAAGT ATAAAAACAC TGCTCTAGTG 
GTATGGAGAC CCTCAGACAC TACGGCACCC TGCACCTACT CTCTGGAGAG GAAGACAGAG 
GGTGAGAATA ACTGGCTGAT AGTCGCCACA GGTGTTGCTG ACTGTTACTA TAACGTGTTG 
GATCTTCCGG CCGGCGCTTC ATACAGGTTC AGAGTGGCCT GCGTCAATAA GGCTGGTCAG 
GGACCTTACA GCAGCTTGTC TGAAGTAGTG GTCCTGGACT CAACAGAGCC CCAGAAGTCC 
AGTGGCACAG TTGTAGTCAA AACGCTTTCA TCAGCTCCCG CTCCTGCAGT GGTAACGTCC 
ACCATGACCC TTCCTGCAAT GAAGCCTGCT GCAATAAAAC CAGCACCATC AACAAATTCA 
GCGCCCTCTA CAGTCCAACC GGTAAAGCCT GCGCCTCCTC CTGCATCATC AACACCTACC 
TATCGTCCCG TGCCTGACAT TCGAACTCCA TCGCCTGGCA CTCCAACCCC TGCCAAAGCC 
AAAAGCACCC TCAACATTAC TATGGCCAAA CCCTCCCCAG CATCCCCTGC AGCTCAACCA 
CAACCACAAC CTCCACCCAA ACCCTCGCCT CCGGTCATTC TGCCCAAACC TCAGACCCCA 
GTCAGTGTGG TTTCACCTCC ATCCCAAACT CCTCCAGTGT CACCTCCACC TCTAGTGTCT 
CCTCCTCCCA TTATTGGTAA ACCAATCTCC TCAGTGCCCA TGTATGTCCC TGCTACAACC 
ACCTCGACTC CAGTGACTCC GGTCACTCCT GTCACCCCAG TCACTCCAGT CACCCCAAAT 
ACGCCCTCTC CTGCCCTGTC GCCTCCAGTG GTGTTGGTTA CAAGTCTGAG TCCTGTAGGG 
GAGGGAACTG GGACACCCAA TCGAATGACC CCTAGTGGAA GAGCCACGCC TTCTGGACGC 
ATCACTCCAT CAGGCCGAAG GACCCCTATT GGAAAGCCTG GAGACTCAAC GCTGAGGCAA 
GGAGTCCCTC AGAAACCTTA CACCTTCATG GATGAGAAAG CAAGGGGTCG CTTTGGTGTG 
ATCAGAGAGT GTCGAGAAAA TGCCACTGGT AACCTGTACA TGGCTAAGAT TGTGCCATAT 
GAGCCAGAGA GCAAGCAGAC TGTATTACAG GAATACGACA TCCTGAAGTC TCTCCATCAT 
GAGAAGATCA TGGCTCTCCA CGAGGCCTAC GTCACTCCAC GCTACCTGGT GCTCATCTCT 
GAGTGCTGCT CTGGCAAAGA ACTACTCCAC AGTCTCATTG ACAGGTTCCG ATACTCTGAG 
GATGATGTGG TTGCATATAT CGTACAGATC CTTCAGGGTC TGGACTACCT GCACAGCCGG 
AGGATCCTGC ATCTCGACAT CAAACCAGAA AACATCATCG TCACTTACAT GAATGTGGTG 
AAGATTATCG ATTTTGGTAG TGCACAGACC TTCAATCCTC TGTTCCTGAA GCAGTTCTCC 
CCACCTATTG GAACACTGGA TTATATGTCT CCAGAGATGC TGAAAGGAGA TGTGGTGGGT 
CCACCAGCTG ACATCTGGAG CATTGGCATA TTGACTTATA TCATGCTTAG CGGGAGGTTG 
CCGTTCACAG AAAATGACCC TGCCGAGACC GAGGCCAGAA TACAGGCAGC CAAGTTTGAC 
CTCAGTAAGC TTTACCAAAA CGTGTCCCAA AGTGCCTCTC TGTTCATCAA AAAGATCCTG 
TGCAGCTACC CTTGGGCCCG TCCGACCATC AAGGACTGCT TTACCAACTC GTGGCTGCAG 
GACGCATATC TGATGCGCCT GCGAAGACAG ACTCTCACAT TCACCACCAC ACGCCTCAAA 
GAGTTCCTGG AGCAGCAGCA GCACATCAGA GCCCAAGAGG CAACAAAGCA CAAAGTCCTC 
CTGCGTTCCT ACCAGAGCAA CCCACAAACC CCATCCACCC CTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spegb ( XM_009304895.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009303170.2 Danio rerio SPEG complex locus b (spegb), transcript variant X6, mRNA.

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CloneID	ODa144106
Clone ID Related Accession (Same CDS sequence)	XM_009304895.3 , XM_021479010.1
Accession Version	XM_009304895.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	striated muscle preferentially expressed protein kinase isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009304895.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGATG TCGCCCCAGA TGCGAGCAAT CGCTGGGCCG CCAGTGAAAG CACAGTGTTG 
GAGAGAGAAG CTCTAGCCCT GGGATCGAGA GCCCCAGGGC CGCAGTCTGC CAGGAGCCCA 
TCTGACGATG TTTTGAGGGA TTCTCCCCCT CAAGTTCTAC CACCCCCTTC CCCCAAATTT 
GGCCGCTCTA CTGCCTCTCC TTTGCTCAGC CGTTCTGGTT CGGGCCCTGC CACACCCCTT 
GCCCCTCGCA AGAAAGTTGT CGTGCCAACT GAATATCAAG ACACAGTACC AGGAGAGTTT 
GAGGAGAAGA TTAAGCAACC CAAATCCTCT GGGATGTCTC AGAGTAGTGC TCAAGACTCT 
CGACCCCAAA CCCCTGTCAG TGAGTATTCC CGTAAGGAGC TTACACTTCG GCCCTCACCA 
AAACTGACAA GAGCTAGCTC CAAAATCTTT GAGAAGGTGC GAGTCTTCGA AGAGCGCAGA 
AGAAGCATTG ATAACCCTGA AGGATCTATA TCTGGACGTT CTTGGGCTGG ATTTAACAGA 
GCTTCATCTA TCGACTCTGA TGAAGGAGGG AGCAGGTTGG GCATCTCCAG GGAGAGCTCC 
AAAGAAGACC TTCGTGAGGC TTTGAAGGCA GATGCAGCCC AACGAAGGAC CATGTTTAAA 
CAGCGGGCTG CTTCCCTGGA GGACAGACCT CGTTACACCC AGAAGGTGCA GGACATTGAA 
AACAAGTTCA CAGAGGAGCT CCAGCGCATC AAAAAGCTTG TCGGCAAACC ACACATGAAG 
AAATCCTTCT CAACAGAGCA GCTCTCTACT TGTCACAGAA GCAGGCAGCC TGTAAGAAAG 
TTAGAGCCCA TCCCACCTCA GGTCCTTCAA AAGCTGCAGG ATAGAGAACG TGCTCAGCTA 
GCTCAGCTAG AGCAAGAGAT GAGAGAGCGA GACCAGGCCA AAGAACGATC ACCTCCGAAA 
TCACCGTCTC GCAGAAGGAA AGACCATCTG GCACAACAAC CACCAGAAAG AGCTGAGACC 
AAGGTGGTAA GCACAACTGG ACTTGAACCA TCCCCTCCAG AAGCCATGTC ACTCTCTGAT 
TTACCAGGTC AAAGATCTCC AAGATTTCGG GGCCCAAGCC CAAGCAGAGA TAGTACACGA 
AGGTCTCCAA CCGTAGAGAT TAGTGCCATG GCTGAGGAGC GCCCTCGAGG AAGAGCTGAA 
TCTCCTTCAA GAAATGTTTT GGAGATGACG TTACGCAAAG TGGAGCCAAG ACCTGCAAGT 
CCACTGGTAA AGAGGGTGGT TCGTGTTCAA GAAGTTCCAC AAGCAAACGA TCAGCCTATG 
CATAGAAAGA CGCCAATAGA GGTCTCGCTA AGAAAACTGG AAAGAAGGCC TGAAAGTCCC 
CTGGTGCAAG GAGAAACCGT AGCCATTCAA GACTTCCCTG TTCAAGCTCC CCCCAAACCT 
CCAAGAGTTT CCTCATCTTC CTCGTCTGAG GAGAAGATGG AGCTGGAAGT GACTCCTCCA 
CCACCCCCAC TAAAACTCAC TATACCAAAA ATTATTGTTG AAGAAGAGCC CATGGAGACA 
GACACACCTG TTGAGAAGAC TGAAAAGGCT GTGAAAGTTG GCACTGGTAA AGATGAAAAA 
CATCAAAGAT CCAGAGGAAG AGGACGCAGA CAAAGACCAA TGTCCCCTGA ATTAGAATCT 
TCGGACGACT CCTATGTGTC TGCAGGAGAG GATCCACTAG AAGCTCCTGT GTTCGAGTTT 
CCACTTCAGG ACACTGTGGC CTCCACTGGA ACGGAGGTGC TATTAAAATG CATCATCAGT 
GGCACCCCTC TCCCTGAAGT TATATGGAAG AAAGACAACA CTGAGGTCAA GAACAGTCCC 
ACTCATGTTG TAAAGATAGA GGGGGAACGC CACTCCTTGC TCATCAAATG GACAAAGCCA 
AGTGATGCAG GAACCTACAC AGTTACCGCT GTCAATGAGG TCGGTGAGGT GTCCAGCAGT 
GCTACACTTT TCATCAAACC AGAGCCCAGT CAGGACCATC GTGGTAACCT TGGTGTCCCT 
ATGGATGTCA GCAGTCCCAT CACATCTGAT GAGGAGTATC TCAGTCCACT GGAAGAGAGC 
ATGGACTTCT CCTCCTACAG GGGACCGGAG CCAAGAAAGA TCGTGGACAC TCGATTTAAG 
GAGCCTCCAG CCTTTCTGGC TGTTATTGGA GACCAAAGCG TAATAGAGGG TCAGGAGGTG 
ACCATGTCTG TTCTAGTGAC CGGACAACCT AAACCTATGC TTTACTGGCT GAGAGACAGA 
GTGACCATTA AAACCGGAAC ACGGCATATC GTCCAAGAGA CAGAGGAGGG CAACTTTGAA 
ATGATAATTA AATCAGCACA GAGGTCAGAC ACCGGTGTTT ACACCTGCAA GATCATCAAT 
GAATATGGAA CTAAGCAATG TGAAGGCAAA CTGGAGGTGA AAGCTCGTCC AGTAGAGCCA 
GGTCTGGCCA TCATTCGGCC AGTGAGGGAC GTGATGGTGA AGGCAGGGGA GACCGCTCTG 
TTTGAGTGTC ATGTGATCGG CCCGCAGGAC ACAGATGTTG ACTGGCTGTC AGATGGGAAA 
CTCATCCAGC CTGCTTTGTT GAACTGCAAG ATGCATTTTG ATGGGAAAAG GTGCAGGCTG 
CTTCTAAACT CTGTACATGA GGATGACAGT GGAACGTACA CCTGCAAACT GAGCACAGCT 
AAAGAGGAAC TGACCTCCAG TGCCAAGCTA AAAGTCATAG CATCCATAGA GCCCCTTTTC 
ACTCGTAAGC TGGATGTGCT GGAAGTTATC GAGGGTCGTA ATGCACGATT TGACTGCAAG 
GTCAGCGGGA CGCCGCCGCC TCAAGTCATA TGGAGCCACT TCGATCGTCC GCTCGAGGAA 
AACGAAGATA TCAGGATCTT AAAAGAAGGA GGACGGCACT CATTAATCAT CTCTCATGTT 
AGTAATGAAG ATGAGGGCCT CTACACGGTT GCAGCGCGAA ACTCACACGG GGAAGACGAA 
TGTGCAGCGG AGCTCTATGT GCAGGAGCCC AGACCAGCAA TATCTTCTCA AATAGCGAAG 
CTGGAAAAAA TGCCTTCCAT TCCCGAAGAA CCCGAAGTTC CTGAAAATGA GATTGAGCGT 
TTCACCATGC CTGACTTCGT AAAGCCGCTT TATGATATGG ATGTAGTGGA GGGCAGAGAG 
GCTGTGCTCA GGTGCAAAGT GGCCGGCTTG CCGTACCCCA CCATAACATG GTTTCACAAT 
GGCAAGAGAA TTGACAGCAC GGAGGACAGA AAGATGACAC AGTTCAGAGA TGTTCACAGC 
CTGGTGGTCC GCTGTGTTTG TCATGCTCAC GGAGGGGTTT ACAAGTGTGT CATATCCAAT 
AAAGTGGGCA AGGCCACCTG CTATTCTCAT CTTTACGTGA CAGACATCCT TCCTGACCCT 
CCAGATGGTG CCCCAGTCGT TGAGTCCATC ACTGGCAAGA CTATCACGCT AAGCTGGAAG 
AAACCCAAAA GACTGGATCC GTCCATTGAC CCCAGCTCCC TGATGTATGC CATTCAGCAA 
CAGGCCCTGG GGTCGATCCA GTGGACCATC ATCGCTTCTT GCCTGAAACA GACCACCTAT 
ACCATCAGCA ATCTGTCTAA AGGTGTCCGC TACGCCTTCA GGGTGCTATC AATCACCAGC 
AAGGCCTTTA GCAAGCCGTC GCCTGCCACC GAGCCAATGC AGCTGCTAGA TAGAGGTCCG 
TACGTCCAGG AGGCTCCGGT AATCATTGAC AAGCCAGATA TCGTGTATAT GGTGGAGAAG 
CAGCCCTTGA GCATCACAGT CACTCTCAAC CACGTCAACG CATCGGTCAC ATGGAAGAGA 
GGAGGAGTGA CATTATCTAA CCGACCGGGT CTTTTTGAGT TGAGCATGCC TGATGATGAC 
CAGCACACAC TGAAGCTGTG TAAGGTGAAG AGCAGTGATG TTGGCCAGCT CACCTGCATT 
GCCAACAACA AGTACGGCAG CGACTCCTGT GTCCTCACCC TGGAGATGGC AGTGGCACCA 
ACTTTTGAGA CAATTATGGA AGACCTGGAT GTGAATGTAG GCGAGACTCC TCGATTTGCA 
GTGGTGGTTG AAGGAAAGCC CGTCCCAGAC ATCCTCTGGT ACAAGGGAGA CACCCTTCTG 
TCCGAAAGCA GCCATTTCAC ATTTGTGTAT GATGACAATG AATGTTCTCT AGTGGTGCTC 
AACACTCAAG CAGACGACTC AGGAGTGTAC ACTTGTACTG CGAAGAATCT GGCCGGTTCT 
GTGTCCTGCA AGGCTGAGCT GACCGTCCAC ACCACCAAAC AGTACAAAGA CGACCCGATG 
GAGGATGAGG CGTCCATCCT CAGAAAGATG CGGAGGCTCA CTGATTACTA TGATGTGCAC 
AAGGAAATTG GGAGAGGGGC TTTCTCATAT GTGAAGCGGG TGATACAGAA GGCAGGAAAG 
CTGGAATACG CAGCCAAGTT TATTTCTGCT CGGGCTAAAC GAAAGGCATC CGCTCTGAGA 
GAACTGAACA TCCTCTCTCA TCTGGACCAT GAGAGGATCC TCTATTTTCA TGATGCCTTT 
GAGAAGAAGA ATGCTGTCAT CATCATTACA GAGCTATGCC ATGAGGAGCT TCTCGATAGG 
CTTACAAAGA AATCAACCAT CTTGGAGTCC GAGATCCGAT CCAGCGTGAG GCAGCTGTTG 
GAGGGTATTA ACTATCTCCA TCAGCTTGAC ATACTTCATC TAGACATCAA GCCTGATAAT 
ATCCTCATGG CAGACCACAG TAGCGATCAG ATCCGCATAT GTGATTTTGG AAATGCTGTA 
AAGTTCATGC CTGATGAGGC ACAGTACTGC AAATATGGCA CTCCAGAATT TGTCGCCCCA 
GAGATTGTTA ACCAGACACC CGTCTCCAAG GCAACGGACA TCTGGCCCAT TGGTGTTCTG 
ACATACTTAT GTCTTACTGG AGTTTCTCCT TTTGCTGGGG AAAACGACCG CAGCTCTGTT 
CTCAACATCA GGAATTATAA TGTGGCTTTT GAAGAAAGCA TGTTTACAGA CCTCTGCCAT 
GAAGCCAAGG GATTCGTCAT CAAGCTGCTG GTGGCTGATA GATTGAGGCC TGATGCTAAT 
GAATGTCTTC GCCACCCCTG GTTCAAGACC CTCAACAAGG GTAAAAGCAT CAGTACAGAG 
TCCCTTAAAA AGTTTTTGTC CAGAAGAAAA TGGCAGCGAT CGCTTATCAG CTACAAATCT 
AAAATGGTGA TGAGGTCTGT ACCTGAGCTA CTGGATGACT CCTCCAGCCA TATTTCCATT 
GCTGTTCCGC GACATCTGAA AAAAGGCTCA CCACCACTTT CATCATCTTC AGATTCAGAT 
GAAGACATCG ATGAGTTGCC TTTTATTCCC ATGCCACTCA ACGTGGAATT CTCAGGATCT 
AGAATGTCAC TGACCGAAAT TGTAGGTGAT GATGACATGA CAGGTAAATT GAATGGAAAT 
GCTGAGAGGC CTGCTTCACT CCATCCGAAG TCCGAGCCAA TGGAAGTGGA GATGTCAGAG 
AGAGAAGGTG GAGATCCATC TGCTAGGGGT AGAGCCAAGA AGAGGACTAC AACAGAAGAT 
GAAAAAGGGT CGTCTGATGA GGAACCATCA CAACTGGCCA AAAGAGCAGA ACTGGCAAAG 
AGACCAATGC GCAGAGGTTC AAGCATGGAG TCAGATGAGC CAGAGGGAGG ACGTCGCAGA 
GGAGAGTTAC GCAGAGGGAG TTCGGCTGAC AGTGCCTTAC AGCTCCATAT CAAACCAGAG 
GAGGGCACAG AAGAAAGTCC TACTGATGGA AGTAGAATTC TACAAAAGTC TGTATCTATG 
GAGCTTCCAA GGAGAAGCCC AAGTCCAGGT GCTGCAAAGC TTAGCCAGGA AGACTATGCT 
CTGAAACTGG AACTGATGAG GCAGCGACTA CTCAGGGGTG GCTCTGCTGA CAACAAGATG 
AGTGGATTAC GAGGCCCATT GCTGGAGACA CTAGGTATGG GAGATGAGAA GCGGTTTGTG 
CGGGGATCTC GTCTAGGTAA CCCTCCACTT GTTAGAGCGG CATCTAGTGA GTCACCTAGA 
GATGACATCC CAAAGACAAA GATTCTCCGC AAGAGTGCTT CCTTTAGCCA GGGGGATTCA 
GAGCCCATGC CCCTGCACAG AAGAATCGGA GCACCTCTAG AGATTCCTCT AGCACAAATA 
GAGGAGAGAC GCCTACAGGA AGCAATATCC ATGTCTGCTC TCACAGAGCC TGTCAAAGAT 
TCTCGCCCAA TCACTCCAAA ACTTCAAATG CCTGAAGAAG AAAGACATGA AAAGAAGAAA 
ACTGAAGAAA AAGAATCTGT CTACAAGGAC AATAAACCTG AAGAGGTTAT TGCTGTGTCA 
AAGACTGAGG AAGAAGGAAG ACGTGTTGAT CAGAAATCTT GGGATTCTGA TGCACAGAAA 
AAGGACCAGA GCAAGTCAGA GGAAACCAGG GAAGAGTCTG AAAAAGTGGC TGAAATTGAA 
AAAAAGACCT CTATAGTGGA CGAAACAATT GAGGAAGAGG AAACAAGAAA AGATGAGGAG 
CCAGAGAAAG AAACTGAGCC TCCTACTCCA AAGATTGAGG TTAAGGAATA TGAGGGTGAT 
GTAAAGGTAT TACCAAAGTC TCCAGAGTTT ATCATTTCCA CAAACTCAAT CATGGTGACC 
CCAACAGCTG CCCCAGCATC CAGTCCCCAG AGTGTATCAA CCTATGTTAC CCCATCTCCC 
CCTGCACGCA CAGTGCTCCC AGATGGTAGG ATGTCCGCAT ATGCCAGTAT TATGCAGACT 
ATCATGGTAC CATCTGTCCC AGCAAATACC CAATCTGACA CTGTTCCTAT TCCTACACCA 
ACTTCCACTC CACCATCAGT TCCAGTCTAT GCACCCATTT CCAGACACAT CTCAGAACCA 
ACCCTTTCAT CAACTTCTTC ATCCAAATCA GATCTTCACC AAACAACTGA GCATCCTGCA 
GTCTTTTCTC GGGTTGCCTC CACTGAACAC CCTCCCAAAG AGCCAAGTCC TCCTAAGACA 
CCCACTCGCG CCTCCCCTAA GCGAGAGCCA TCTCCTGGAA GCAACATCCA GGACCTTGCA 
TCCGAGGAGG TTTTTGAAGC TCGCTTCAAG AAACGTGAGT CTTCTCTAAC TCGTAGTCTC 
AAACGTTTCT CATTTCAGAA GACTGAAGAC AAGCCCTCCA CTGTTTCTCC TGAAACTGCT 
GAAGAAATGT ATCGCCCTGG ACCAACTGGT GCTCCTTTAG AGTTTGTGCC AAGGAGGTTG 
GAGGAGAAGT CCAAATCAGT ACAAGATCTT CGAGAGGCAG AGAAGGACCC TGGGTTCATG 
AGGCGACTCT CCATGCGCTT GAAAAGGACC CCATCAGTGG AAAAGCAGGA GGAGAAGCTC 
AAAGAGGAGG AGGCTTCAGC ACCCAGGCGT CGTCTTTCTT GGGCACTGGG ACGGAGGGGA 
TCCCAAGAGA AGAAGGAGAT TGAGATGATG AGGTTGGATG GGGGTCCTGA GCCTCCCCCA 
GAGCCTGAGT CTAAAGCACC TTCTGAGTCA CCTATTCTTG CCATGCGCAA GAAGATTGGC 
AGCACCATGG CAAGCTTATC TACAAGAATT AGGAGCCATT CTGAGGACAG GAAAGATGAC 
AGTGAAGGCA AGATGGAGAC CAAGAGGACT CCTATATTCA ACAAACTAAG GCGTTCCACT 
TCTGAGGGGG GCAGTCTGAA GAGAATGGGT ATCCCACAAA ACCAACTGGC TGCTCAGTCA 
GGGCAAGGGA CCTCATCAGA ATCTCTTGAT TCTATGTCAA GCATCCAGTC AGAATCAGCT 
ATTAAAAATG CAGAACTTGA CCGCAGATCA CGGTGGGACC GATGGGGCTT GACTCGAGGG 
AAAAAGGACA AGACAGTTTC CCAACCAGAC ATCCCAGCTT CCATTGCTAG AGAGAATGGT 
TCTCTACGGT CTCGCCAATA TACACGTCTA ACATCTGACT TCCCTCCGGT GTTCCATATC 
AAACTGAGGG ACCATGTACT CTTGGAGGGA GATCCTGTCA CTCTCAGCTG CCTCCCTGCT 
GGCAGTCCTC ACCCACACAT CTCATGGATG AAAGACAAGA AGCCACTTGA GATTGACCCC 
AGGATGAATA TGATATCTTG CCCCGATGGG AGACAACTGC TGATGATTAT GAAGACCACC 
AAGAAGGATG CAGGCCTCTA TGAATGTGTG GCTGCAAACC CACTGGCCAC AGTCACCAGC 
TCATGCGTTG TGTCCCTCGC TCGTCTGCCT AATAGTCCTG GGACACCCGA GGTTCCTCAG 
AAGTATAAAA ACACTGCTCT AGTGGTATGG AGACCCTCAG ACACTACGGC ACCCTGCACC 
TACTCTCTGG AGAGGAAGAC AGAGGGTGAG AATAACTGGC TGATAGTCGC CACAGGTGTT 
GCTGACTGTT ACTATAACGT GTTGGATCTT CCGGCCGGCG CTTCATACAG GTTCAGAGTG 
GCCTGCGTCA ATAAGGCTGG TCAGGGACCT TACAGCAGCT TGTCTGAAGT AGTGGTCCTG 
GACTCAACAG AGCCCCAGAA GTCCAGTGGC ACAGTTGTAG TCAAAACGCT TTCATCAGCT 
CCCGCTCCTG CAGTGGTAAC GTCCACCATG ACCCTTCCTG CAATGAAGCC TGCTGCAATA 
AAACCAGCAC CATCAACAAA TTCAGCGCCC TCTACAGTCC AACCGGTAAA GCCTGCGCCT 
CCTCCTGCAT CATCAACACC TACCTATCGT CCCGTGCCTG ACATTCGAAC TCCATCGCCT 
GGCACTCCAA CCCCTGCCAA AGCCAAAAGC ACCCTCAACA TTACTATGGC CAAACCCTCC 
CCAGCATCCC CTGCAGCTCA ACCACAACCA CAACCTCCAC CCAAACCCTC GCCTCCGGTC 
ATTCTGCCCA AACCTCAGAC CCCAGTCAGT GTGGTTTCAC CTCCATCCCA AACTCCTCCA 
GTGTCACCTC CACCTCTAGT GTCTCCTCCT CCCATTATTG GTAAACCAAT CTCCTCAGTG 
CCCATGTATG TCCCTGCTAC AACCACCTCG ACTCCAGTGA CTCCGGTCAC TCCTGTCACC 
CCAGTCACTC CAGTCACCCC AAATACGCCC TCTCCTGCCC TGTCGCCTCC AGTGGTGTTG 
GTTACAAGTC TGAGTCCTGT AGGGGAGGGA ACTGGGACAC CCAATCGAAT GACCCCTAGT 
GGAAGAGCCA CGCCTTCTGG ACGCATCACT CCATCAGGCC GAAGGACCCC TATTGGAAAG 
CCTGGAGACT CAACGCTGAG GCAAGGAGTC CCTCAGAAAC CTTACACCTT CATGGATGAG 
AAAGCAAGGG GTCGCTTTGG TGTGATCAGA GAGTGTCGAG AAAATGCCAC TGGTAACCTG 
TACATGGCTA AGATTGTGCC ATATGAGCCA GAGAGCAAGC AGACTGTATT ACAGGAATAC 
GACATCCTGA AGTCTCTCCA TCATGAGAAG ATCATGGCTC TCCACGAGGC CTACGTCACT 
CCACGCTACC TGGTGCTCAT CTCTGAGTGC TGCTCTGGCA AAGAACTACT CCACAGTCTC 
ATTGACAGGT TCCGATACTC TGAGGATGAT GTGGTTGCAT ATATCGTACA GATCCTTCAG 
GGTCTGGACT ACCTGCACAG CCGGAGGATC CTGCATCTCG ACATCAAACC AGAAAACATC 
ATCGTCACTT ACATGAATGT GGTGAAGATT ATCGATTTTG GTAGTGCACA GACCTTCAAT 
CCTCTGTTCC TGAAGCAGTT CTCCCCACCT ATTGGAACAC TGGATTATAT GTCTCCAGAG 
ATGCTGAAAG GAGATGTGGT GGGTCCACCA GCTGACATCT GGAGCATTGG CATATTGACT 
TATATCATGC TTAGCGGGAG GTTGCCGTTC ACAGAAAATG ACCCTGCCGA GACCGAGGCC 
AGAATACAGG CAGCCAAGTT TGACCTCAGT AAGCTTTACC AAAACGTGTC CCAAAGTGCC 
TCTCTGTTCA TCAAAAAGAT CCTGTGCAGC TACCCTTGGG CCCGTCCGAC CATCAAGGAC 
TGCTTTACCA ACTCGTGGCT GCAGGACGCA TATCTGATGC GCCTGCGAAG ACAGACTCTC 
ACATTCACCA CCACACGCCT CAAAGAGTTC CTGGAGCAGC AGCAGCACAT CAGAGCCCAA 
GAGGCAACAA AGCACAAAGT CCTCCTGCGT TCCTACCAGA GCAACCCACA AACCCCATCC 
ACCCCTCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303170.2
CDS	195..10406
Translation	MMDVAPDASNRWAASESTVLEREALALGSRAPGPQSARSPSDDVLRDSPPQVLPPPSPKFGRSTASPLLSRSGSGPATPLAPRKKVVVPTEYQDTVPGEFEEKIKQPKSSGMSQSSAQDSRPQTPVSEYSRKELTLRPSPKLTRASSKIFEKVRVFEERRRSIDNPEGSISGRSWAGFNRASSIDSDEGGSRLGISRESSKEDLREALKADAAQRRTMFKQRAASLEDRPRYTQKVQDIENKFTEELQRIKKLVGKPHMKKSFSTEQLSTCHRSRQPVRKLEPIPPQVLQKLQDRERAQLAQLEQEMRERDQAKERSPPKSPSRRRKDHLAQQPPERAETKVVSTTGLEPSPPEAMSLSDLPGQRSPRFRGPSPSRDSTRRSPTVEISAMAEERPRGRAESPSRNVLEMTLRKVEPRPASPLVKRVVRVQEVPQANDQPMHRKTPIEVSLRKLERRPESPLVQGETVAIQDFPVQAPPKPPRVSSSSSSEEKMELEVTPPPPPLKLTIPKIIVEEEPMETDTPVEKTEKAVKVGTGKDEKHQRSRGRGRRQRPMSPELESSDDSYVSAGEDPLEAPVFEFPLQDTVASTGTEVLLKCIISGTPLPEVIWKKDNTEVKNSPTHVVKIEGERHSLLIKWTKPSDAGTYTVTAVNEVGEVSSSATLFIKPEPSQDHRGNLGVPMDVSSPITSDEEYLSPLEESMDFSSYRGPEPRKIVDTRFKEPPAFLAVIGDQSVIEGQEVTMSVLVTGQPKPMLYWLRDRVTIKTGTRHIVQETEEGNFEMIIKSAQRSDTGVYTCKIINEYGTKQCEGKLEVKARPVEPGLAIIRPVRDVMVKAGETALFECHVIGPQDTDVDWLSDGKLIQPALLNCKMHFDGKRCRLLLNSVHEDDSGTYTCKLSTAKEELTSSAKLKVIASIEPLFTRKLDVLEVIEGRNARFDCKVSGTPPPQVIWSHFDRPLEENEDIRILKEGGRHSLIISHVSNEDEGLYTVAARNSHGEDECAAELYVQEPRPAISSQIAKLEKMPSIPEEPEVPENEIERFTMPDFVKPLYDMDVVEGREAVLRCKVAGLPYPTITWFHNGKRIDSTEDRKMTQFRDVHSLVVRCVCHAHGGVYKCVISNKVGKATCYSHLYVTDILPDPPDGAPVVESITGKTITLSWKKPKRLDPSIDPSSLMYAIQQQALGSIQWTIIASCLKQTTYTISNLSKGVRYAFRVLSITSKAFSKPSPATEPMQLLDRGPYVQEAPVIIDKPDIVYMVEKQPLSITVTLNHVNASVTWKRGGVTLSNRPGLFELSMPDDDQHTLKLCKVKSSDVGQLTCIANNKYGSDSCVLTLEMAVAPTFETIMEDLDVNVGETPRFAVVVEGKPVPDILWYKGDTLLSESSHFTFVYDDNECSLVVLNTQADDSGVYTCTAKNLAGSVSCKAELTVHTTKQYKDDPMEDEASILRKMRRLTDYYDVHKEIGRGAFSYVKRVIQKAGKLEYAAKFISARAKRKASALRELNILSHLDHERILYFHDAFEKKNAVIIITELCHEELLDRLTKKSTILESEIRSSVRQLLEGINYLHQLDILHLDIKPDNILMADHSSDQIRICDFGNAVKFMPDEAQYCKYGTPEFVAPEIVNQTPVSKATDIWPIGVLTYLCLTGVSPFAGENDRSSVLNIRNYNVAFEESMFTDLCHEAKGFVIKLLVADRLRPDANECLRHPWFKTLNKGKSISTESLKKFLSRRKWQRSLISYKSKMVMRSVPELLDDSSSHISIAVPRHLKKGSPPLSSSSDSDEDIDELPFIPMPLNVEFSGSRMSLTEIVGDDDMTGKLNGNAERPASLHPKSEPMEVEMSEREGGDPSARGRAKKRTTTEDEKGSSDEEPSQLAKRAELAKRPMRRGSSMESDEPEGGRRRGELRRGSSADSALQLHIKPEEGTEESPTDGSRILQKSVSMELPRRSPSPGAAKLSQEDYALKLELMRQRLLRGGSADNKMSGLRGPLLETLGMGDEKRFVRGSRLGNPPLVRAASSESPRDDIPKTKILRKSASFSQGDSEPMPLHRRIGAPLEIPLAQIEERRLQEAISMSALTEPVKDSRPITPKLQMPEEERHEKKKTEEKESVYKDNKPEEVIAVSKTEEEGRRVDQKSWDSDAQKKDQSKSEETREESEKVAEIEKKTSIVDETIEEEETRKDEEPEKETEPPTPKIEVKEYEGDVKVLPKSPEFIISTNSIMVTPTAAPASSPQSVSTYVTPSPPARTVLPDGRMSAYASIMQTIMVPSVPANTQSDTVPIPTPTSTPPSVPVYAPISRHISEPTLSSTSSSKSDLHQTTEHPAVFSRVASTEHPPKEPSPPKTPTRASPKREPSPGSNIQDLASEEVFEARFKKRESSLTRSLKRFSFQKTEDKPSTVSPETAEEMYRPGPTGAPLEFVPRRLEEKSKSVQDLREAEKDPGFMRRLSMRLKRTPSVEKQEEKLKEEEASAPRRRLSWALGRRGSQEKKEIEMMRLDGGPEPPPEPESKAPSESPILAMRKKIGSTMASLSTRIRSHSEDRKDDSEGKMETKRTPIFNKLRRSTSEGGSLKRMGIPQNQLAAQSGQGTSSESLDSMSSIQSESAIKNAELDRRSRWDRWGLTRGKKDKTVSQPDIPASIARENGSLRSRQYTRLTSDFPPVFHIKLRDHVLLEGDPVTLSCLPAGSPHPHISWMKDKKPLEIDPRMNMISCPDGRQLLMIMKTTKKDAGLYECVAANPLATVTSSCVVSLARLPNSPGTPEVPQKYKNTALVVWRPSDTTAPCTYSLERKTEGENNWLIVATGVADCYYNVLDLPAGASYRFRVACVNKAGQGPYSSLSEVVVLDSTEPQKSSGTVVVKTLSSAPAPAVVTSTMTLPAMKPAAIKPAPSTNSAPSTVQPVKPAPPPASSTPTYRPVPDIRTPSPGTPTPAKAKSTLNITMAKPSPASPAAQPQPQPPPKPSPPVILPKPQTPVSVVSPPSQTPPVSPPPLVSPPPIIGKPISSVPMYVPATTTSTPVTPVTPVTPVTPVTPNTPSPALSPPVVLVTSLSPVGEGTGTPNRMTPSGRATPSGRITPSGRRTPIGKPGDSTLRQGVPQKPYTFMDEKARGRFGVIRECRENATGNLYMAKIVPYEPESKQTVLQEYDILKSLHHEKIMALHEAYVTPRYLVLISECCSGKELLHSLIDRFRYSEDDVVAYIVQILQGLDYLHSRRILHLDIKPENIIVTYMNVVKIIDFGSAQTFNPLFLKQFSPPIGTLDYMSPEMLKGDVVGPPADIWSIGILTYIMLSGRLPFTENDPAETEARIQAAKFDLSKLYQNVSQSASLFIKKILCSYPWARPTIKDCFTNSWLQDAYLMRLRRQTLTFTTTRLKEFLEQQQHIRAQEATKHKVLLRSYQSNPQTPSTPQ

Target ORF information:

RefSeq Version	XM_009304895.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SPEG complex locus b (spegb), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304895.3

ORF Insert Sequence:

ATGATGGATG TCGCCCCAGA TGCGAGCAAT CGCTGGGCCG CCAGTGAAAG CACAGTGTTG 
GAGAGAGAAG CTCTAGCCCT GGGATCGAGA GCCCCAGGGC CGCAGTCTGC CAGGAGCCCA 
TCTGACGATG TTTTGAGGGA TTCTCCCCCT CAAGTTCTAC CACCCCCTTC CCCCAAATTT 
GGCCGCTCTA CTGCCTCTCC TTTGCTCAGC CGTTCTGGTT CGGGCCCTGC CACACCCCTT 
GCCCCTCGCA AGAAAGTTGT CGTGCCAACT GAATATCAAG ACACAGTACC AGGAGAGTTT 
GAGGAGAAGA TTAAGCAACC CAAATCCTCT GGGATGTCTC AGAGTAGTGC TCAAGACTCT 
CGACCCCAAA CCCCTGTCAG TGAGTATTCC CGTAAGGAGC TTACACTTCG GCCCTCACCA 
AAACTGACAA GAGCTAGCTC CAAAATCTTT GAGAAGGTGC GAGTCTTCGA AGAGCGCAGA 
AGAAGCATTG ATAACCCTGA AGGATCTATA TCTGGACGTT CTTGGGCTGG ATTTAACAGA 
GCTTCATCTA TCGACTCTGA TGAAGGAGGG AGCAGGTTGG GCATCTCCAG GGAGAGCTCC 
AAAGAAGACC TTCGTGAGGC TTTGAAGGCA GATGCAGCCC AACGAAGGAC CATGTTTAAA 
CAGCGGGCTG CTTCCCTGGA GGACAGACCT CGTTACACCC AGAAGGTGCA GGACATTGAA 
AACAAGTTCA CAGAGGAGCT CCAGCGCATC AAAAAGCTTG TCGGCAAACC ACACATGAAG 
AAATCCTTCT CAACAGAGCA GCTCTCTACT TGTCACAGAA GCAGGCAGCC TGTAAGAAAG 
TTAGAGCCCA TCCCACCTCA GGTCCTTCAA AAGCTGCAGG ATAGAGAACG TGCTCAGCTA 
GCTCAGCTAG AGCAAGAGAT GAGAGAGCGA GACCAGGCCA AAGAACGATC ACCTCCGAAA 
TCACCGTCTC GCAGAAGGAA AGACCATCTG GCACAACAAC CACCAGAAAG AGCTGAGACC 
AAGGTGGTAA GCACAACTGG ACTTGAACCA TCCCCTCCAG AAGCCATGTC ACTCTCTGAT 
TTACCAGGTC AAAGATCTCC AAGATTTCGG GGCCCAAGCC CAAGCAGAGA TAGTACACGA 
AGGTCTCCAA CCGTAGAGAT TAGTGCCATG GCTGAGGAGC GCCCTCGAGG AAGAGCTGAA 
TCTCCTTCAA GAAATGTTTT GGAGATGACG TTACGCAAAG TGGAGCCAAG ACCTGCAAGT 
CCACTGGTAA AGAGGGTGGT TCGTGTTCAA GAAGTTCCAC AAGCAAACGA TCAGCCTATG 
CATAGAAAGA CGCCAATAGA GGTCTCGCTA AGAAAACTGG AAAGAAGGCC TGAAAGTCCC 
CTGGTGCAAG GAGAAACCGT AGCCATTCAA GACTTCCCTG TTCAAGCTCC CCCCAAACCT 
CCAAGAGTTT CCTCATCTTC CTCGTCTGAG GAGAAGATGG AGCTGGAAGT GACTCCTCCA 
CCACCCCCAC TAAAACTCAC TATACCAAAA ATTATTGTTG AAGAAGAGCC CATGGAGACA 
GACACACCTG TTGAGAAGAC TGAAAAGGCT GTGAAAGTTG GCACTGGTAA AGATGAAAAA 
CATCAAAGAT CCAGAGGAAG AGGACGCAGA CAAAGACCAA TGTCCCCTGA ATTAGAATCT 
TCGGACGACT CCTATGTGTC TGCAGGAGAG GATCCACTAG AAGCTCCTGT GTTCGAGTTT 
CCACTTCAGG ACACTGTGGC CTCCACTGGA ACGGAGGTGC TATTAAAATG CATCATCAGT 
GGCACCCCTC TCCCTGAAGT TATATGGAAG AAAGACAACA CTGAGGTCAA GAACAGTCCC 
ACTCATGTTG TAAAGATAGA GGGGGAACGC CACTCCTTGC TCATCAAATG GACAAAGCCA 
AGTGATGCAG GAACCTACAC AGTTACCGCT GTCAATGAGG TCGGTGAGGT GTCCAGCAGT 
GCTACACTTT TCATCAAACC AGAGCCCAGT CAGGACCATC GTGGTAACCT TGGTGTCCCT 
ATGGATGTCA GCAGTCCCAT CACATCTGAT GAGGAGTATC TCAGTCCACT GGAAGAGAGC 
ATGGACTTCT CCTCCTACAG GGGACCGGAG CCAAGAAAGA TCGTGGACAC TCGATTTAAG 
GAGCCTCCAG CCTTTCTGGC TGTTATTGGA GACCAAAGCG TAATAGAGGG TCAGGAGGTG 
ACCATGTCTG TTCTAGTGAC CGGACAACCT AAACCTATGC TTTACTGGCT GAGAGACAGA 
GTGACCATTA AAACCGGAAC ACGGCATATC GTCCAAGAGA CAGAGGAGGG CAACTTTGAA 
ATGATAATTA AATCAGCACA GAGGTCAGAC ACCGGTGTTT ACACCTGCAA GATCATCAAT 
GAATATGGAA CTAAGCAATG TGAAGGCAAA CTGGAGGTGA AAGCTCGTCC AGTAGAGCCA 
GGTCTGGCCA TCATTCGGCC AGTGAGGGAC GTGATGGTGA AGGCAGGGGA GACCGCTCTG 
TTTGAGTGTC ATGTGATCGG CCCGCAGGAC ACAGATGTTG ACTGGCTGTC AGATGGGAAA 
CTCATCCAGC CTGCTTTGTT GAACTGCAAG ATGCATTTTG ATGGGAAAAG GTGCAGGCTG 
CTTCTAAACT CTGTACATGA GGATGACAGT GGAACGTACA CCTGCAAACT GAGCACAGCT 
AAAGAGGAAC TGACCTCCAG TGCCAAGCTA AAAGTCATAG CATCCATAGA GCCCCTTTTC 
ACTCGTAAGC TGGATGTGCT GGAAGTTATC GAGGGTCGTA ATGCACGATT TGACTGCAAG 
GTCAGCGGGA CGCCGCCGCC TCAAGTCATA TGGAGCCACT TCGATCGTCC GCTCGAGGAA 
AACGAAGATA TCAGGATCTT AAAAGAAGGA GGACGGCACT CATTAATCAT CTCTCATGTT 
AGTAATGAAG ATGAGGGCCT CTACACGGTT GCAGCGCGAA ACTCACACGG GGAAGACGAA 
TGTGCAGCGG AGCTCTATGT GCAGGAGCCC AGACCAGCAA TATCTTCTCA AATAGCGAAG 
CTGGAAAAAA TGCCTTCCAT TCCCGAAGAA CCCGAAGTTC CTGAAAATGA GATTGAGCGT 
TTCACCATGC CTGACTTCGT AAAGCCGCTT TATGATATGG ATGTAGTGGA GGGCAGAGAG 
GCTGTGCTCA GGTGCAAAGT GGCCGGCTTG CCGTACCCCA CCATAACATG GTTTCACAAT 
GGCAAGAGAA TTGACAGCAC GGAGGACAGA AAGATGACAC AGTTCAGAGA TGTTCACAGC 
CTGGTGGTCC GCTGTGTTTG TCATGCTCAC GGAGGGGTTT ACAAGTGTGT CATATCCAAT 
AAAGTGGGCA AGGCCACCTG CTATTCTCAT CTTTACGTGA CAGACATCCT TCCTGACCCT 
CCAGATGGTG CCCCAGTCGT TGAGTCCATC ACTGGCAAGA CTATCACGCT AAGCTGGAAG 
AAACCCAAAA GACTGGATCC GTCCATTGAC CCCAGCTCCC TGATGTATGC CATTCAGCAA 
CAGGCCCTGG GGTCGATCCA GTGGACCATC ATCGCTTCTT GCCTGAAACA GACCACCTAT 
ACCATCAGCA ATCTGTCTAA AGGTGTCCGC TACGCCTTCA GGGTGCTATC AATCACCAGC 
AAGGCCTTTA GCAAGCCGTC GCCTGCCACC GAGCCAATGC AGCTGCTAGA TAGAGGTCCG 
TACGTCCAGG AGGCTCCGGT AATCATTGAC AAGCCAGATA TCGTGTATAT GGTGGAGAAG 
CAGCCCTTGA GCATCACAGT CACTCTCAAC CACGTCAACG CATCGGTCAC ATGGAAGAGA 
GGAGGAGTGA CATTATCTAA CCGACCGGGT CTTTTTGAGT TGAGCATGCC TGATGATGAC 
CAGCACACAC TGAAGCTGTG TAAGGTGAAG AGCAGTGATG TTGGCCAGCT CACCTGCATT 
GCCAACAACA AGTACGGCAG CGACTCCTGT GTCCTCACCC TGGAGATGGC AGTGGCACCA 
ACTTTTGAGA CAATTATGGA AGACCTGGAT GTGAATGTAG GCGAGACTCC TCGATTTGCA 
GTGGTGGTTG AAGGAAAGCC CGTCCCAGAC ATCCTCTGGT ACAAGGGAGA CACCCTTCTG 
TCCGAAAGCA GCCATTTCAC ATTTGTGTAT GATGACAATG AATGTTCTCT AGTGGTGCTC 
AACACTCAAG CAGACGACTC AGGAGTGTAC ACTTGTACTG CGAAGAATCT GGCCGGTTCT 
GTGTCCTGCA AGGCTGAGCT GACCGTCCAC ACCACCAAAC AGTACAAAGA CGACCCGATG 
GAGGATGAGG CGTCCATCCT CAGAAAGATG CGGAGGCTCA CTGATTACTA TGATGTGCAC 
AAGGAAATTG GGAGAGGGGC TTTCTCATAT GTGAAGCGGG TGATACAGAA GGCAGGAAAG 
CTGGAATACG CAGCCAAGTT TATTTCTGCT CGGGCTAAAC GAAAGGCATC CGCTCTGAGA 
GAACTGAACA TCCTCTCTCA TCTGGACCAT GAGAGGATCC TCTATTTTCA TGATGCCTTT 
GAGAAGAAGA ATGCTGTCAT CATCATTACA GAGCTATGCC ATGAGGAGCT TCTCGATAGG 
CTTACAAAGA AATCAACCAT CTTGGAGTCC GAGATCCGAT CCAGCGTGAG GCAGCTGTTG 
GAGGGTATTA ACTATCTCCA TCAGCTTGAC ATACTTCATC TAGACATCAA GCCTGATAAT 
ATCCTCATGG CAGACCACAG TAGCGATCAG ATCCGCATAT GTGATTTTGG AAATGCTGTA 
AAGTTCATGC CTGATGAGGC ACAGTACTGC AAATATGGCA CTCCAGAATT TGTCGCCCCA 
GAGATTGTTA ACCAGACACC CGTCTCCAAG GCAACGGACA TCTGGCCCAT TGGTGTTCTG 
ACATACTTAT GTCTTACTGG AGTTTCTCCT TTTGCTGGGG AAAACGACCG CAGCTCTGTT 
CTCAACATCA GGAATTATAA TGTGGCTTTT GAAGAAAGCA TGTTTACAGA CCTCTGCCAT 
GAAGCCAAGG GATTCGTCAT CAAGCTGCTG GTGGCTGATA GATTGAGGCC TGATGCTAAT 
GAATGTCTTC GCCACCCCTG GTTCAAGACC CTCAACAAGG GTAAAAGCAT CAGTACAGAG 
TCCCTTAAAA AGTTTTTGTC CAGAAGAAAA TGGCAGCGAT CGCTTATCAG CTACAAATCT 
AAAATGGTGA TGAGGTCTGT ACCTGAGCTA CTGGATGACT CCTCCAGCCA TATTTCCATT 
GCTGTTCCGC GACATCTGAA AAAAGGCTCA CCACCACTTT CATCATCTTC AGATTCAGAT 
GAAGACATCG ATGAGTTGCC TTTTATTCCC ATGCCACTCA ACGTGGAATT CTCAGGATCT 
AGAATGTCAC TGACCGAAAT TGTAGGTGAT GATGACATGA CAGGTAAATT GAATGGAAAT 
GCTGAGAGGC CTGCTTCACT CCATCCGAAG TCCGAGCCAA TGGAAGTGGA GATGTCAGAG 
AGAGAAGGTG GAGATCCATC TGCTAGGGGT AGAGCCAAGA AGAGGACTAC AACAGAAGAT 
GAAAAAGGGT CGTCTGATGA GGAACCATCA CAACTGGCCA AAAGAGCAGA ACTGGCAAAG 
AGACCAATGC GCAGAGGTTC AAGCATGGAG TCAGATGAGC CAGAGGGAGG ACGTCGCAGA 
GGAGAGTTAC GCAGAGGGAG TTCGGCTGAC AGTGCCTTAC AGCTCCATAT CAAACCAGAG 
GAGGGCACAG AAGAAAGTCC TACTGATGGA AGTAGAATTC TACAAAAGTC TGTATCTATG 
GAGCTTCCAA GGAGAAGCCC AAGTCCAGGT GCTGCAAAGC TTAGCCAGGA AGACTATGCT 
CTGAAACTGG AACTGATGAG GCAGCGACTA CTCAGGGGTG GCTCTGCTGA CAACAAGATG 
AGTGGATTAC GAGGCCCATT GCTGGAGACA CTAGGTATGG GAGATGAGAA GCGGTTTGTG 
CGGGGATCTC GTCTAGGTAA CCCTCCACTT GTTAGAGCGG CATCTAGTGA GTCACCTAGA 
GATGACATCC CAAAGACAAA GATTCTCCGC AAGAGTGCTT CCTTTAGCCA GGGGGATTCA 
GAGCCCATGC CCCTGCACAG AAGAATCGGA GCACCTCTAG AGATTCCTCT AGCACAAATA 
GAGGAGAGAC GCCTACAGGA AGCAATATCC ATGTCTGCTC TCACAGAGCC TGTCAAAGAT 
TCTCGCCCAA TCACTCCAAA ACTTCAAATG CCTGAAGAAG AAAGACATGA AAAGAAGAAA 
ACTGAAGAAA AAGAATCTGT CTACAAGGAC AATAAACCTG AAGAGGTTAT TGCTGTGTCA 
AAGACTGAGG AAGAAGGAAG ACGTGTTGAT CAGAAATCTT GGGATTCTGA TGCACAGAAA 
AAGGACCAGA GCAAGTCAGA GGAAACCAGG GAAGAGTCTG AAAAAGTGGC TGAAATTGAA 
AAAAAGACCT CTATAGTGGA CGAAACAATT GAGGAAGAGG AAACAAGAAA AGATGAGGAG 
CCAGAGAAAG AAACTGAGCC TCCTACTCCA AAGATTGAGG TTAAGGAATA TGAGGGTGAT 
GTAAAGGTAT TACCAAAGTC TCCAGAGTTT ATCATTTCCA CAAACTCAAT CATGGTGACC 
CCAACAGCTG CCCCAGCATC CAGTCCCCAG AGTGTATCAA CCTATGTTAC CCCATCTCCC 
CCTGCACGCA CAGTGCTCCC AGATGGTAGG ATGTCCGCAT ATGCCAGTAT TATGCAGACT 
ATCATGGTAC CATCTGTCCC AGCAAATACC CAATCTGACA CTGTTCCTAT TCCTACACCA 
ACTTCCACTC CACCATCAGT TCCAGTCTAT GCACCCATTT CCAGACACAT CTCAGAACCA 
ACCCTTTCAT CAACTTCTTC ATCCAAATCA GATCTTCACC AAACAACTGA GCATCCTGCA 
GTCTTTTCTC GGGTTGCCTC CACTGAACAC CCTCCCAAAG AGCCAAGTCC TCCTAAGACA 
CCCACTCGCG CCTCCCCTAA GCGAGAGCCA TCTCCTGGAA GCAACATCCA GGACCTTGCA 
TCCGAGGAGG TTTTTGAAGC TCGCTTCAAG AAACGTGAGT CTTCTCTAAC TCGTAGTCTC 
AAACGTTTCT CATTTCAGAA GACTGAAGAC AAGCCCTCCA CTGTTTCTCC TGAAACTGCT 
GAAGAAATGT ATCGCCCTGG ACCAACTGGT GCTCCTTTAG AGTTTGTGCC AAGGAGGTTG 
GAGGAGAAGT CCAAATCAGT ACAAGATCTT CGAGAGGCAG AGAAGGACCC TGGGTTCATG 
AGGCGACTCT CCATGCGCTT GAAAAGGACC CCATCAGTGG AAAAGCAGGA GGAGAAGCTC 
AAAGAGGAGG AGGCTTCAGC ACCCAGGCGT CGTCTTTCTT GGGCACTGGG ACGGAGGGGA 
TCCCAAGAGA AGAAGGAGAT TGAGATGATG AGGTTGGATG GGGGTCCTGA GCCTCCCCCA 
GAGCCTGAGT CTAAAGCACC TTCTGAGTCA CCTATTCTTG CCATGCGCAA GAAGATTGGC 
AGCACCATGG CAAGCTTATC TACAAGAATT AGGAGCCATT CTGAGGACAG GAAAGATGAC 
AGTGAAGGCA AGATGGAGAC CAAGAGGACT CCTATATTCA ACAAACTAAG GCGTTCCACT 
TCTGAGGGGG GCAGTCTGAA GAGAATGGGT ATCCCACAAA ACCAACTGGC TGCTCAGTCA 
GGGCAAGGGA CCTCATCAGA ATCTCTTGAT TCTATGTCAA GCATCCAGTC AGAATCAGCT 
ATTAAAAATG CAGAACTTGA CCGCAGATCA CGGTGGGACC GATGGGGCTT GACTCGAGGG 
AAAAAGGACA AGACAGTTTC CCAACCAGAC ATCCCAGCTT CCATTGCTAG AGAGAATGGT 
TCTCTACGGT CTCGCCAATA TACACGTCTA ACATCTGACT TCCCTCCGGT GTTCCATATC 
AAACTGAGGG ACCATGTACT CTTGGAGGGA GATCCTGTCA CTCTCAGCTG CCTCCCTGCT 
GGCAGTCCTC ACCCACACAT CTCATGGATG AAAGACAAGA AGCCACTTGA GATTGACCCC 
AGGATGAATA TGATATCTTG CCCCGATGGG AGACAACTGC TGATGATTAT GAAGACCACC 
AAGAAGGATG CAGGCCTCTA TGAATGTGTG GCTGCAAACC CACTGGCCAC AGTCACCAGC 
TCATGCGTTG TGTCCCTCGC TCGTCTGCCT AATAGTCCTG GGACACCCGA GGTTCCTCAG 
AAGTATAAAA ACACTGCTCT AGTGGTATGG AGACCCTCAG ACACTACGGC ACCCTGCACC 
TACTCTCTGG AGAGGAAGAC AGAGGGTGAG AATAACTGGC TGATAGTCGC CACAGGTGTT 
GCTGACTGTT ACTATAACGT GTTGGATCTT CCGGCCGGCG CTTCATACAG GTTCAGAGTG 
GCCTGCGTCA ATAAGGCTGG TCAGGGACCT TACAGCAGCT TGTCTGAAGT AGTGGTCCTG 
GACTCAACAG AGCCCCAGAA GTCCAGTGGC ACAGTTGTAG TCAAAACGCT TTCATCAGCT 
CCCGCTCCTG CAGTGGTAAC GTCCACCATG ACCCTTCCTG CAATGAAGCC TGCTGCAATA 
AAACCAGCAC CATCAACAAA TTCAGCGCCC TCTACAGTCC AACCGGTAAA GCCTGCGCCT 
CCTCCTGCAT CATCAACACC TACCTATCGT CCCGTGCCTG ACATTCGAAC TCCATCGCCT 
GGCACTCCAA CCCCTGCCAA AGCCAAAAGC ACCCTCAACA TTACTATGGC CAAACCCTCC 
CCAGCATCCC CTGCAGCTCA ACCACAACCA CAACCTCCAC CCAAACCCTC GCCTCCGGTC 
ATTCTGCCCA AACCTCAGAC CCCAGTCAGT GTGGTTTCAC CTCCATCCCA AACTCCTCCA 
GTGTCACCTC CACCTCTAGT GTCTCCTCCT CCCATTATTG GTAAACCAAT CTCCTCAGTG 
CCCATGTATG TCCCTGCTAC AACCACCTCG ACTCCAGTGA CTCCGGTCAC TCCTGTCACC 
CCAGTCACTC CAGTCACCCC AAATACGCCC TCTCCTGCCC TGTCGCCTCC AGTGGTGTTG 
GTTACAAGTC TGAGTCCTGT AGGGGAGGGA ACTGGGACAC CCAATCGAAT GACCCCTAGT 
GGAAGAGCCA CGCCTTCTGG ACGCATCACT CCATCAGGCC GAAGGACCCC TATTGGAAAG 
CCTGGAGACT CAACGCTGAG GCAAGGAGTC CCTCAGAAAC CTTACACCTT CATGGATGAG 
AAAGCAAGGG GTCGCTTTGG TGTGATCAGA GAGTGTCGAG AAAATGCCAC TGGTAACCTG 
TACATGGCTA AGATTGTGCC ATATGAGCCA GAGAGCAAGC AGACTGTATT ACAGGAATAC 
GACATCCTGA AGTCTCTCCA TCATGAGAAG ATCATGGCTC TCCACGAGGC CTACGTCACT 
CCACGCTACC TGGTGCTCAT CTCTGAGTGC TGCTCTGGCA AAGAACTACT CCACAGTCTC 
ATTGACAGGT TCCGATACTC TGAGGATGAT GTGGTTGCAT ATATCGTACA GATCCTTCAG 
GGTCTGGACT ACCTGCACAG CCGGAGGATC CTGCATCTCG ACATCAAACC AGAAAACATC 
ATCGTCACTT ACATGAATGT GGTGAAGATT ATCGATTTTG GTAGTGCACA GACCTTCAAT 
CCTCTGTTCC TGAAGCAGTT CTCCCCACCT ATTGGAACAC TGGATTATAT GTCTCCAGAG 
ATGCTGAAAG GAGATGTGGT GGGTCCACCA GCTGACATCT GGAGCATTGG CATATTGACT 
TATATCATGC TTAGCGGGAG GTTGCCGTTC ACAGAAAATG ACCCTGCCGA GACCGAGGCC 
AGAATACAGG CAGCCAAGTT TGACCTCAGT AAGCTTTACC AAAACGTGTC CCAAAGTGCC 
TCTCTGTTCA TCAAAAAGAT CCTGTGCAGC TACCCTTGGG CCCGTCCGAC CATCAAGGAC 
TGCTTTACCA ACTCGTGGCT GCAGGACGCA TATCTGATGC GCCTGCGAAG ACAGACTCTC 
ACATTCACCA CCACACGCCT CAAAGAGTTC CTGGAGCAGC AGCAGCACAT CAGAGCCCAA 
GAGGCAACAA AGCACAAAGT CCTCCTGCGT TCCTACCAGA GCAACCCACA AACCCCATCC 
ACCCCTCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spegb ( XM_021479010.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334685.1 Danio rerio SPEG complex locus b (spegb), transcript variant X5, mRNA.

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CloneID	ODa144106
Clone ID Related Accession (Same CDS sequence)	XM_009304895.3 , XM_021479010.1
Accession Version	XM_021479010.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	striated muscle preferentially expressed protein kinase isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGATG TCGCCCCAGA TGCGAGCAAT CGCTGGGCCG CCAGTGAAAG CACAGTGTTG 
GAGAGAGAAG CTCTAGCCCT GGGATCGAGA GCCCCAGGGC CGCAGTCTGC CAGGAGCCCA 
TCTGACGATG TTTTGAGGGA TTCTCCCCCT CAAGTTCTAC CACCCCCTTC CCCCAAATTT 
GGCCGCTCTA CTGCCTCTCC TTTGCTCAGC CGTTCTGGTT CGGGCCCTGC CACACCCCTT 
GCCCCTCGCA AGAAAGTTGT CGTGCCAACT GAATATCAAG ACACAGTACC AGGAGAGTTT 
GAGGAGAAGA TTAAGCAACC CAAATCCTCT GGGATGTCTC AGAGTAGTGC TCAAGACTCT 
CGACCCCAAA CCCCTGTCAG TGAGTATTCC CGTAAGGAGC TTACACTTCG GCCCTCACCA 
AAACTGACAA GAGCTAGCTC CAAAATCTTT GAGAAGGTGC GAGTCTTCGA AGAGCGCAGA 
AGAAGCATTG ATAACCCTGA AGGATCTATA TCTGGACGTT CTTGGGCTGG ATTTAACAGA 
GCTTCATCTA TCGACTCTGA TGAAGGAGGG AGCAGGTTGG GCATCTCCAG GGAGAGCTCC 
AAAGAAGACC TTCGTGAGGC TTTGAAGGCA GATGCAGCCC AACGAAGGAC CATGTTTAAA 
CAGCGGGCTG CTTCCCTGGA GGACAGACCT CGTTACACCC AGAAGGTGCA GGACATTGAA 
AACAAGTTCA CAGAGGAGCT CCAGCGCATC AAAAAGCTTG TCGGCAAACC ACACATGAAG 
AAATCCTTCT CAACAGAGCA GCTCTCTACT TGTCACAGAA GCAGGCAGCC TGTAAGAAAG 
TTAGAGCCCA TCCCACCTCA GGTCCTTCAA AAGCTGCAGG ATAGAGAACG TGCTCAGCTA 
GCTCAGCTAG AGCAAGAGAT GAGAGAGCGA GACCAGGCCA AAGAACGATC ACCTCCGAAA 
TCACCGTCTC GCAGAAGGAA AGACCATCTG GCACAACAAC CACCAGAAAG AGCTGAGACC 
AAGGTGGTAA GCACAACTGG ACTTGAACCA TCCCCTCCAG AAGCCATGTC ACTCTCTGAT 
TTACCAGGTC AAAGATCTCC AAGATTTCGG GGCCCAAGCC CAAGCAGAGA TAGTACACGA 
AGGTCTCCAA CCGTAGAGAT TAGTGCCATG GCTGAGGAGC GCCCTCGAGG AAGAGCTGAA 
TCTCCTTCAA GAAATGTTTT GGAGATGACG TTACGCAAAG TGGAGCCAAG ACCTGCAAGT 
CCACTGGTAA AGAGGGTGGT TCGTGTTCAA GAAGTTCCAC AAGCAAACGA TCAGCCTATG 
CATAGAAAGA CGCCAATAGA GGTCTCGCTA AGAAAACTGG AAAGAAGGCC TGAAAGTCCC 
CTGGTGCAAG GAGAAACCGT AGCCATTCAA GACTTCCCTG TTCAAGCTCC CCCCAAACCT 
CCAAGAGTTT CCTCATCTTC CTCGTCTGAG GAGAAGATGG AGCTGGAAGT GACTCCTCCA 
CCACCCCCAC TAAAACTCAC TATACCAAAA ATTATTGTTG AAGAAGAGCC CATGGAGACA 
GACACACCTG TTGAGAAGAC TGAAAAGGCT GTGAAAGTTG GCACTGGTAA AGATGAAAAA 
CATCAAAGAT CCAGAGGAAG AGGACGCAGA CAAAGACCAA TGTCCCCTGA ATTAGAATCT 
TCGGACGACT CCTATGTGTC TGCAGGAGAG GATCCACTAG AAGCTCCTGT GTTCGAGTTT 
CCACTTCAGG ACACTGTGGC CTCCACTGGA ACGGAGGTGC TATTAAAATG CATCATCAGT 
GGCACCCCTC TCCCTGAAGT TATATGGAAG AAAGACAACA CTGAGGTCAA GAACAGTCCC 
ACTCATGTTG TAAAGATAGA GGGGGAACGC CACTCCTTGC TCATCAAATG GACAAAGCCA 
AGTGATGCAG GAACCTACAC AGTTACCGCT GTCAATGAGG TCGGTGAGGT GTCCAGCAGT 
GCTACACTTT TCATCAAACC AGAGCCCAGT CAGGACCATC GTGGTAACCT TGGTGTCCCT 
ATGGATGTCA GCAGTCCCAT CACATCTGAT GAGGAGTATC TCAGTCCACT GGAAGAGAGC 
ATGGACTTCT CCTCCTACAG GGGACCGGAG CCAAGAAAGA TCGTGGACAC TCGATTTAAG 
GAGCCTCCAG CCTTTCTGGC TGTTATTGGA GACCAAAGCG TAATAGAGGG TCAGGAGGTG 
ACCATGTCTG TTCTAGTGAC CGGACAACCT AAACCTATGC TTTACTGGCT GAGAGACAGA 
GTGACCATTA AAACCGGAAC ACGGCATATC GTCCAAGAGA CAGAGGAGGG CAACTTTGAA 
ATGATAATTA AATCAGCACA GAGGTCAGAC ACCGGTGTTT ACACCTGCAA GATCATCAAT 
GAATATGGAA CTAAGCAATG TGAAGGCAAA CTGGAGGTGA AAGCTCGTCC AGTAGAGCCA 
GGTCTGGCCA TCATTCGGCC AGTGAGGGAC GTGATGGTGA AGGCAGGGGA GACCGCTCTG 
TTTGAGTGTC ATGTGATCGG CCCGCAGGAC ACAGATGTTG ACTGGCTGTC AGATGGGAAA 
CTCATCCAGC CTGCTTTGTT GAACTGCAAG ATGCATTTTG ATGGGAAAAG GTGCAGGCTG 
CTTCTAAACT CTGTACATGA GGATGACAGT GGAACGTACA CCTGCAAACT GAGCACAGCT 
AAAGAGGAAC TGACCTCCAG TGCCAAGCTA AAAGTCATAG CATCCATAGA GCCCCTTTTC 
ACTCGTAAGC TGGATGTGCT GGAAGTTATC GAGGGTCGTA ATGCACGATT TGACTGCAAG 
GTCAGCGGGA CGCCGCCGCC TCAAGTCATA TGGAGCCACT TCGATCGTCC GCTCGAGGAA 
AACGAAGATA TCAGGATCTT AAAAGAAGGA GGACGGCACT CATTAATCAT CTCTCATGTT 
AGTAATGAAG ATGAGGGCCT CTACACGGTT GCAGCGCGAA ACTCACACGG GGAAGACGAA 
TGTGCAGCGG AGCTCTATGT GCAGGAGCCC AGACCAGCAA TATCTTCTCA AATAGCGAAG 
CTGGAAAAAA TGCCTTCCAT TCCCGAAGAA CCCGAAGTTC CTGAAAATGA GATTGAGCGT 
TTCACCATGC CTGACTTCGT AAAGCCGCTT TATGATATGG ATGTAGTGGA GGGCAGAGAG 
GCTGTGCTCA GGTGCAAAGT GGCCGGCTTG CCGTACCCCA CCATAACATG GTTTCACAAT 
GGCAAGAGAA TTGACAGCAC GGAGGACAGA AAGATGACAC AGTTCAGAGA TGTTCACAGC 
CTGGTGGTCC GCTGTGTTTG TCATGCTCAC GGAGGGGTTT ACAAGTGTGT CATATCCAAT 
AAAGTGGGCA AGGCCACCTG CTATTCTCAT CTTTACGTGA CAGACATCCT TCCTGACCCT 
CCAGATGGTG CCCCAGTCGT TGAGTCCATC ACTGGCAAGA CTATCACGCT AAGCTGGAAG 
AAACCCAAAA GACTGGATCC GTCCATTGAC CCCAGCTCCC TGATGTATGC CATTCAGCAA 
CAGGCCCTGG GGTCGATCCA GTGGACCATC ATCGCTTCTT GCCTGAAACA GACCACCTAT 
ACCATCAGCA ATCTGTCTAA AGGTGTCCGC TACGCCTTCA GGGTGCTATC AATCACCAGC 
AAGGCCTTTA GCAAGCCGTC GCCTGCCACC GAGCCAATGC AGCTGCTAGA TAGAGGTCCG 
TACGTCCAGG AGGCTCCGGT AATCATTGAC AAGCCAGATA TCGTGTATAT GGTGGAGAAG 
CAGCCCTTGA GCATCACAGT CACTCTCAAC CACGTCAACG CATCGGTCAC ATGGAAGAGA 
GGAGGAGTGA CATTATCTAA CCGACCGGGT CTTTTTGAGT TGAGCATGCC TGATGATGAC 
CAGCACACAC TGAAGCTGTG TAAGGTGAAG AGCAGTGATG TTGGCCAGCT CACCTGCATT 
GCCAACAACA AGTACGGCAG CGACTCCTGT GTCCTCACCC TGGAGATGGC AGTGGCACCA 
ACTTTTGAGA CAATTATGGA AGACCTGGAT GTGAATGTAG GCGAGACTCC TCGATTTGCA 
GTGGTGGTTG AAGGAAAGCC CGTCCCAGAC ATCCTCTGGT ACAAGGGAGA CACCCTTCTG 
TCCGAAAGCA GCCATTTCAC ATTTGTGTAT GATGACAATG AATGTTCTCT AGTGGTGCTC 
AACACTCAAG CAGACGACTC AGGAGTGTAC ACTTGTACTG CGAAGAATCT GGCCGGTTCT 
GTGTCCTGCA AGGCTGAGCT GACCGTCCAC ACCACCAAAC AGTACAAAGA CGACCCGATG 
GAGGATGAGG CGTCCATCCT CAGAAAGATG CGGAGGCTCA CTGATTACTA TGATGTGCAC 
AAGGAAATTG GGAGAGGGGC TTTCTCATAT GTGAAGCGGG TGATACAGAA GGCAGGAAAG 
CTGGAATACG CAGCCAAGTT TATTTCTGCT CGGGCTAAAC GAAAGGCATC CGCTCTGAGA 
GAACTGAACA TCCTCTCTCA TCTGGACCAT GAGAGGATCC TCTATTTTCA TGATGCCTTT 
GAGAAGAAGA ATGCTGTCAT CATCATTACA GAGCTATGCC ATGAGGAGCT TCTCGATAGG 
CTTACAAAGA AATCAACCAT CTTGGAGTCC GAGATCCGAT CCAGCGTGAG GCAGCTGTTG 
GAGGGTATTA ACTATCTCCA TCAGCTTGAC ATACTTCATC TAGACATCAA GCCTGATAAT 
ATCCTCATGG CAGACCACAG TAGCGATCAG ATCCGCATAT GTGATTTTGG AAATGCTGTA 
AAGTTCATGC CTGATGAGGC ACAGTACTGC AAATATGGCA CTCCAGAATT TGTCGCCCCA 
GAGATTGTTA ACCAGACACC CGTCTCCAAG GCAACGGACA TCTGGCCCAT TGGTGTTCTG 
ACATACTTAT GTCTTACTGG AGTTTCTCCT TTTGCTGGGG AAAACGACCG CAGCTCTGTT 
CTCAACATCA GGAATTATAA TGTGGCTTTT GAAGAAAGCA TGTTTACAGA CCTCTGCCAT 
GAAGCCAAGG GATTCGTCAT CAAGCTGCTG GTGGCTGATA GATTGAGGCC TGATGCTAAT 
GAATGTCTTC GCCACCCCTG GTTCAAGACC CTCAACAAGG GTAAAAGCAT CAGTACAGAG 
TCCCTTAAAA AGTTTTTGTC CAGAAGAAAA TGGCAGCGAT CGCTTATCAG CTACAAATCT 
AAAATGGTGA TGAGGTCTGT ACCTGAGCTA CTGGATGACT CCTCCAGCCA TATTTCCATT 
GCTGTTCCGC GACATCTGAA AAAAGGCTCA CCACCACTTT CATCATCTTC AGATTCAGAT 
GAAGACATCG ATGAGTTGCC TTTTATTCCC ATGCCACTCA ACGTGGAATT CTCAGGATCT 
AGAATGTCAC TGACCGAAAT TGTAGGTGAT GATGACATGA CAGGTAAATT GAATGGAAAT 
GCTGAGAGGC CTGCTTCACT CCATCCGAAG TCCGAGCCAA TGGAAGTGGA GATGTCAGAG 
AGAGAAGGTG GAGATCCATC TGCTAGGGGT AGAGCCAAGA AGAGGACTAC AACAGAAGAT 
GAAAAAGGGT CGTCTGATGA GGAACCATCA CAACTGGCCA AAAGAGCAGA ACTGGCAAAG 
AGACCAATGC GCAGAGGTTC AAGCATGGAG TCAGATGAGC CAGAGGGAGG ACGTCGCAGA 
GGAGAGTTAC GCAGAGGGAG TTCGGCTGAC AGTGCCTTAC AGCTCCATAT CAAACCAGAG 
GAGGGCACAG AAGAAAGTCC TACTGATGGA AGTAGAATTC TACAAAAGTC TGTATCTATG 
GAGCTTCCAA GGAGAAGCCC AAGTCCAGGT GCTGCAAAGC TTAGCCAGGA AGACTATGCT 
CTGAAACTGG AACTGATGAG GCAGCGACTA CTCAGGGGTG GCTCTGCTGA CAACAAGATG 
AGTGGATTAC GAGGCCCATT GCTGGAGACA CTAGGTATGG GAGATGAGAA GCGGTTTGTG 
CGGGGATCTC GTCTAGGTAA CCCTCCACTT GTTAGAGCGG CATCTAGTGA GTCACCTAGA 
GATGACATCC CAAAGACAAA GATTCTCCGC AAGAGTGCTT CCTTTAGCCA GGGGGATTCA 
GAGCCCATGC CCCTGCACAG AAGAATCGGA GCACCTCTAG AGATTCCTCT AGCACAAATA 
GAGGAGAGAC GCCTACAGGA AGCAATATCC ATGTCTGCTC TCACAGAGCC TGTCAAAGAT 
TCTCGCCCAA TCACTCCAAA ACTTCAAATG CCTGAAGAAG AAAGACATGA AAAGAAGAAA 
ACTGAAGAAA AAGAATCTGT CTACAAGGAC AATAAACCTG AAGAGGTTAT TGCTGTGTCA 
AAGACTGAGG AAGAAGGAAG ACGTGTTGAT CAGAAATCTT GGGATTCTGA TGCACAGAAA 
AAGGACCAGA GCAAGTCAGA GGAAACCAGG GAAGAGTCTG AAAAAGTGGC TGAAATTGAA 
AAAAAGACCT CTATAGTGGA CGAAACAATT GAGGAAGAGG AAACAAGAAA AGATGAGGAG 
CCAGAGAAAG AAACTGAGCC TCCTACTCCA AAGATTGAGG TTAAGGAATA TGAGGGTGAT 
GTAAAGGTAT TACCAAAGTC TCCAGAGTTT ATCATTTCCA CAAACTCAAT CATGGTGACC 
CCAACAGCTG CCCCAGCATC CAGTCCCCAG AGTGTATCAA CCTATGTTAC CCCATCTCCC 
CCTGCACGCA CAGTGCTCCC AGATGGTAGG ATGTCCGCAT ATGCCAGTAT TATGCAGACT 
ATCATGGTAC CATCTGTCCC AGCAAATACC CAATCTGACA CTGTTCCTAT TCCTACACCA 
ACTTCCACTC CACCATCAGT TCCAGTCTAT GCACCCATTT CCAGACACAT CTCAGAACCA 
ACCCTTTCAT CAACTTCTTC ATCCAAATCA GATCTTCACC AAACAACTGA GCATCCTGCA 
GTCTTTTCTC GGGTTGCCTC CACTGAACAC CCTCCCAAAG AGCCAAGTCC TCCTAAGACA 
CCCACTCGCG CCTCCCCTAA GCGAGAGCCA TCTCCTGGAA GCAACATCCA GGACCTTGCA 
TCCGAGGAGG TTTTTGAAGC TCGCTTCAAG AAACGTGAGT CTTCTCTAAC TCGTAGTCTC 
AAACGTTTCT CATTTCAGAA GACTGAAGAC AAGCCCTCCA CTGTTTCTCC TGAAACTGCT 
GAAGAAATGT ATCGCCCTGG ACCAACTGGT GCTCCTTTAG AGTTTGTGCC AAGGAGGTTG 
GAGGAGAAGT CCAAATCAGT ACAAGATCTT CGAGAGGCAG AGAAGGACCC TGGGTTCATG 
AGGCGACTCT CCATGCGCTT GAAAAGGACC CCATCAGTGG AAAAGCAGGA GGAGAAGCTC 
AAAGAGGAGG AGGCTTCAGC ACCCAGGCGT CGTCTTTCTT GGGCACTGGG ACGGAGGGGA 
TCCCAAGAGA AGAAGGAGAT TGAGATGATG AGGTTGGATG GGGGTCCTGA GCCTCCCCCA 
GAGCCTGAGT CTAAAGCACC TTCTGAGTCA CCTATTCTTG CCATGCGCAA GAAGATTGGC 
AGCACCATGG CAAGCTTATC TACAAGAATT AGGAGCCATT CTGAGGACAG GAAAGATGAC 
AGTGAAGGCA AGATGGAGAC CAAGAGGACT CCTATATTCA ACAAACTAAG GCGTTCCACT 
TCTGAGGGGG GCAGTCTGAA GAGAATGGGT ATCCCACAAA ACCAACTGGC TGCTCAGTCA 
GGGCAAGGGA CCTCATCAGA ATCTCTTGAT TCTATGTCAA GCATCCAGTC AGAATCAGCT 
ATTAAAAATG CAGAACTTGA CCGCAGATCA CGGTGGGACC GATGGGGCTT GACTCGAGGG 
AAAAAGGACA AGACAGTTTC CCAACCAGAC ATCCCAGCTT CCATTGCTAG AGAGAATGGT 
TCTCTACGGT CTCGCCAATA TACACGTCTA ACATCTGACT TCCCTCCGGT GTTCCATATC 
AAACTGAGGG ACCATGTACT CTTGGAGGGA GATCCTGTCA CTCTCAGCTG CCTCCCTGCT 
GGCAGTCCTC ACCCACACAT CTCATGGATG AAAGACAAGA AGCCACTTGA GATTGACCCC 
AGGATGAATA TGATATCTTG CCCCGATGGG AGACAACTGC TGATGATTAT GAAGACCACC 
AAGAAGGATG CAGGCCTCTA TGAATGTGTG GCTGCAAACC CACTGGCCAC AGTCACCAGC 
TCATGCGTTG TGTCCCTCGC TCGTCTGCCT AATAGTCCTG GGACACCCGA GGTTCCTCAG 
AAGTATAAAA ACACTGCTCT AGTGGTATGG AGACCCTCAG ACACTACGGC ACCCTGCACC 
TACTCTCTGG AGAGGAAGAC AGAGGGTGAG AATAACTGGC TGATAGTCGC CACAGGTGTT 
GCTGACTGTT ACTATAACGT GTTGGATCTT CCGGCCGGCG CTTCATACAG GTTCAGAGTG 
GCCTGCGTCA ATAAGGCTGG TCAGGGACCT TACAGCAGCT TGTCTGAAGT AGTGGTCCTG 
GACTCAACAG AGCCCCAGAA GTCCAGTGGC ACAGTTGTAG TCAAAACGCT TTCATCAGCT 
CCCGCTCCTG CAGTGGTAAC GTCCACCATG ACCCTTCCTG CAATGAAGCC TGCTGCAATA 
AAACCAGCAC CATCAACAAA TTCAGCGCCC TCTACAGTCC AACCGGTAAA GCCTGCGCCT 
CCTCCTGCAT CATCAACACC TACCTATCGT CCCGTGCCTG ACATTCGAAC TCCATCGCCT 
GGCACTCCAA CCCCTGCCAA AGCCAAAAGC ACCCTCAACA TTACTATGGC CAAACCCTCC 
CCAGCATCCC CTGCAGCTCA ACCACAACCA CAACCTCCAC CCAAACCCTC GCCTCCGGTC 
ATTCTGCCCA AACCTCAGAC CCCAGTCAGT GTGGTTTCAC CTCCATCCCA AACTCCTCCA 
GTGTCACCTC CACCTCTAGT GTCTCCTCCT CCCATTATTG GTAAACCAAT CTCCTCAGTG 
CCCATGTATG TCCCTGCTAC AACCACCTCG ACTCCAGTGA CTCCGGTCAC TCCTGTCACC 
CCAGTCACTC CAGTCACCCC AAATACGCCC TCTCCTGCCC TGTCGCCTCC AGTGGTGTTG 
GTTACAAGTC TGAGTCCTGT AGGGGAGGGA ACTGGGACAC CCAATCGAAT GACCCCTAGT 
GGAAGAGCCA CGCCTTCTGG ACGCATCACT CCATCAGGCC GAAGGACCCC TATTGGAAAG 
CCTGGAGACT CAACGCTGAG GCAAGGAGTC CCTCAGAAAC CTTACACCTT CATGGATGAG 
AAAGCAAGGG GTCGCTTTGG TGTGATCAGA GAGTGTCGAG AAAATGCCAC TGGTAACCTG 
TACATGGCTA AGATTGTGCC ATATGAGCCA GAGAGCAAGC AGACTGTATT ACAGGAATAC 
GACATCCTGA AGTCTCTCCA TCATGAGAAG ATCATGGCTC TCCACGAGGC CTACGTCACT 
CCACGCTACC TGGTGCTCAT CTCTGAGTGC TGCTCTGGCA AAGAACTACT CCACAGTCTC 
ATTGACAGGT TCCGATACTC TGAGGATGAT GTGGTTGCAT ATATCGTACA GATCCTTCAG 
GGTCTGGACT ACCTGCACAG CCGGAGGATC CTGCATCTCG ACATCAAACC AGAAAACATC 
ATCGTCACTT ACATGAATGT GGTGAAGATT ATCGATTTTG GTAGTGCACA GACCTTCAAT 
CCTCTGTTCC TGAAGCAGTT CTCCCCACCT ATTGGAACAC TGGATTATAT GTCTCCAGAG 
ATGCTGAAAG GAGATGTGGT GGGTCCACCA GCTGACATCT GGAGCATTGG CATATTGACT 
TATATCATGC TTAGCGGGAG GTTGCCGTTC ACAGAAAATG ACCCTGCCGA GACCGAGGCC 
AGAATACAGG CAGCCAAGTT TGACCTCAGT AAGCTTTACC AAAACGTGTC CCAAAGTGCC 
TCTCTGTTCA TCAAAAAGAT CCTGTGCAGC TACCCTTGGG CCCGTCCGAC CATCAAGGAC 
TGCTTTACCA ACTCGTGGCT GCAGGACGCA TATCTGATGC GCCTGCGAAG ACAGACTCTC 
ACATTCACCA CCACACGCCT CAAAGAGTTC CTGGAGCAGC AGCAGCACAT CAGAGCCCAA 
GAGGCAACAA AGCACAAAGT CCTCCTGCGT TCCTACCAGA GCAACCCACA AACCCCATCC 
ACCCCTCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334685.1
CDS	209..10420
Translation	MMDVAPDASNRWAASESTVLEREALALGSRAPGPQSARSPSDDVLRDSPPQVLPPPSPKFGRSTASPLLSRSGSGPATPLAPRKKVVVPTEYQDTVPGEFEEKIKQPKSSGMSQSSAQDSRPQTPVSEYSRKELTLRPSPKLTRASSKIFEKVRVFEERRRSIDNPEGSISGRSWAGFNRASSIDSDEGGSRLGISRESSKEDLREALKADAAQRRTMFKQRAASLEDRPRYTQKVQDIENKFTEELQRIKKLVGKPHMKKSFSTEQLSTCHRSRQPVRKLEPIPPQVLQKLQDRERAQLAQLEQEMRERDQAKERSPPKSPSRRRKDHLAQQPPERAETKVVSTTGLEPSPPEAMSLSDLPGQRSPRFRGPSPSRDSTRRSPTVEISAMAEERPRGRAESPSRNVLEMTLRKVEPRPASPLVKRVVRVQEVPQANDQPMHRKTPIEVSLRKLERRPESPLVQGETVAIQDFPVQAPPKPPRVSSSSSSEEKMELEVTPPPPPLKLTIPKIIVEEEPMETDTPVEKTEKAVKVGTGKDEKHQRSRGRGRRQRPMSPELESSDDSYVSAGEDPLEAPVFEFPLQDTVASTGTEVLLKCIISGTPLPEVIWKKDNTEVKNSPTHVVKIEGERHSLLIKWTKPSDAGTYTVTAVNEVGEVSSSATLFIKPEPSQDHRGNLGVPMDVSSPITSDEEYLSPLEESMDFSSYRGPEPRKIVDTRFKEPPAFLAVIGDQSVIEGQEVTMSVLVTGQPKPMLYWLRDRVTIKTGTRHIVQETEEGNFEMIIKSAQRSDTGVYTCKIINEYGTKQCEGKLEVKARPVEPGLAIIRPVRDVMVKAGETALFECHVIGPQDTDVDWLSDGKLIQPALLNCKMHFDGKRCRLLLNSVHEDDSGTYTCKLSTAKEELTSSAKLKVIASIEPLFTRKLDVLEVIEGRNARFDCKVSGTPPPQVIWSHFDRPLEENEDIRILKEGGRHSLIISHVSNEDEGLYTVAARNSHGEDECAAELYVQEPRPAISSQIAKLEKMPSIPEEPEVPENEIERFTMPDFVKPLYDMDVVEGREAVLRCKVAGLPYPTITWFHNGKRIDSTEDRKMTQFRDVHSLVVRCVCHAHGGVYKCVISNKVGKATCYSHLYVTDILPDPPDGAPVVESITGKTITLSWKKPKRLDPSIDPSSLMYAIQQQALGSIQWTIIASCLKQTTYTISNLSKGVRYAFRVLSITSKAFSKPSPATEPMQLLDRGPYVQEAPVIIDKPDIVYMVEKQPLSITVTLNHVNASVTWKRGGVTLSNRPGLFELSMPDDDQHTLKLCKVKSSDVGQLTCIANNKYGSDSCVLTLEMAVAPTFETIMEDLDVNVGETPRFAVVVEGKPVPDILWYKGDTLLSESSHFTFVYDDNECSLVVLNTQADDSGVYTCTAKNLAGSVSCKAELTVHTTKQYKDDPMEDEASILRKMRRLTDYYDVHKEIGRGAFSYVKRVIQKAGKLEYAAKFISARAKRKASALRELNILSHLDHERILYFHDAFEKKNAVIIITELCHEELLDRLTKKSTILESEIRSSVRQLLEGINYLHQLDILHLDIKPDNILMADHSSDQIRICDFGNAVKFMPDEAQYCKYGTPEFVAPEIVNQTPVSKATDIWPIGVLTYLCLTGVSPFAGENDRSSVLNIRNYNVAFEESMFTDLCHEAKGFVIKLLVADRLRPDANECLRHPWFKTLNKGKSISTESLKKFLSRRKWQRSLISYKSKMVMRSVPELLDDSSSHISIAVPRHLKKGSPPLSSSSDSDEDIDELPFIPMPLNVEFSGSRMSLTEIVGDDDMTGKLNGNAERPASLHPKSEPMEVEMSEREGGDPSARGRAKKRTTTEDEKGSSDEEPSQLAKRAELAKRPMRRGSSMESDEPEGGRRRGELRRGSSADSALQLHIKPEEGTEESPTDGSRILQKSVSMELPRRSPSPGAAKLSQEDYALKLELMRQRLLRGGSADNKMSGLRGPLLETLGMGDEKRFVRGSRLGNPPLVRAASSESPRDDIPKTKILRKSASFSQGDSEPMPLHRRIGAPLEIPLAQIEERRLQEAISMSALTEPVKDSRPITPKLQMPEEERHEKKKTEEKESVYKDNKPEEVIAVSKTEEEGRRVDQKSWDSDAQKKDQSKSEETREESEKVAEIEKKTSIVDETIEEEETRKDEEPEKETEPPTPKIEVKEYEGDVKVLPKSPEFIISTNSIMVTPTAAPASSPQSVSTYVTPSPPARTVLPDGRMSAYASIMQTIMVPSVPANTQSDTVPIPTPTSTPPSVPVYAPISRHISEPTLSSTSSSKSDLHQTTEHPAVFSRVASTEHPPKEPSPPKTPTRASPKREPSPGSNIQDLASEEVFEARFKKRESSLTRSLKRFSFQKTEDKPSTVSPETAEEMYRPGPTGAPLEFVPRRLEEKSKSVQDLREAEKDPGFMRRLSMRLKRTPSVEKQEEKLKEEEASAPRRRLSWALGRRGSQEKKEIEMMRLDGGPEPPPEPESKAPSESPILAMRKKIGSTMASLSTRIRSHSEDRKDDSEGKMETKRTPIFNKLRRSTSEGGSLKRMGIPQNQLAAQSGQGTSSESLDSMSSIQSESAIKNAELDRRSRWDRWGLTRGKKDKTVSQPDIPASIARENGSLRSRQYTRLTSDFPPVFHIKLRDHVLLEGDPVTLSCLPAGSPHPHISWMKDKKPLEIDPRMNMISCPDGRQLLMIMKTTKKDAGLYECVAANPLATVTSSCVVSLARLPNSPGTPEVPQKYKNTALVVWRPSDTTAPCTYSLERKTEGENNWLIVATGVADCYYNVLDLPAGASYRFRVACVNKAGQGPYSSLSEVVVLDSTEPQKSSGTVVVKTLSSAPAPAVVTSTMTLPAMKPAAIKPAPSTNSAPSTVQPVKPAPPPASSTPTYRPVPDIRTPSPGTPTPAKAKSTLNITMAKPSPASPAAQPQPQPPPKPSPPVILPKPQTPVSVVSPPSQTPPVSPPPLVSPPPIIGKPISSVPMYVPATTTSTPVTPVTPVTPVTPVTPNTPSPALSPPVVLVTSLSPVGEGTGTPNRMTPSGRATPSGRITPSGRRTPIGKPGDSTLRQGVPQKPYTFMDEKARGRFGVIRECRENATGNLYMAKIVPYEPESKQTVLQEYDILKSLHHEKIMALHEAYVTPRYLVLISECCSGKELLHSLIDRFRYSEDDVVAYIVQILQGLDYLHSRRILHLDIKPENIIVTYMNVVKIIDFGSAQTFNPLFLKQFSPPIGTLDYMSPEMLKGDVVGPPADIWSIGILTYIMLSGRLPFTENDPAETEARIQAAKFDLSKLYQNVSQSASLFIKKILCSYPWARPTIKDCFTNSWLQDAYLMRLRRQTLTFTTTRLKEFLEQQQHIRAQEATKHKVLLRSYQSNPQTPSTPQ

Target ORF information:

RefSeq Version	XM_021479010.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SPEG complex locus b (spegb), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479010.1

ORF Insert Sequence:

ATGATGGATG TCGCCCCAGA TGCGAGCAAT CGCTGGGCCG CCAGTGAAAG CACAGTGTTG 
GAGAGAGAAG CTCTAGCCCT GGGATCGAGA GCCCCAGGGC CGCAGTCTGC CAGGAGCCCA 
TCTGACGATG TTTTGAGGGA TTCTCCCCCT CAAGTTCTAC CACCCCCTTC CCCCAAATTT 
GGCCGCTCTA CTGCCTCTCC TTTGCTCAGC CGTTCTGGTT CGGGCCCTGC CACACCCCTT 
GCCCCTCGCA AGAAAGTTGT CGTGCCAACT GAATATCAAG ACACAGTACC AGGAGAGTTT 
GAGGAGAAGA TTAAGCAACC CAAATCCTCT GGGATGTCTC AGAGTAGTGC TCAAGACTCT 
CGACCCCAAA CCCCTGTCAG TGAGTATTCC CGTAAGGAGC TTACACTTCG GCCCTCACCA 
AAACTGACAA GAGCTAGCTC CAAAATCTTT GAGAAGGTGC GAGTCTTCGA AGAGCGCAGA 
AGAAGCATTG ATAACCCTGA AGGATCTATA TCTGGACGTT CTTGGGCTGG ATTTAACAGA 
GCTTCATCTA TCGACTCTGA TGAAGGAGGG AGCAGGTTGG GCATCTCCAG GGAGAGCTCC 
AAAGAAGACC TTCGTGAGGC TTTGAAGGCA GATGCAGCCC AACGAAGGAC CATGTTTAAA 
CAGCGGGCTG CTTCCCTGGA GGACAGACCT CGTTACACCC AGAAGGTGCA GGACATTGAA 
AACAAGTTCA CAGAGGAGCT CCAGCGCATC AAAAAGCTTG TCGGCAAACC ACACATGAAG 
AAATCCTTCT CAACAGAGCA GCTCTCTACT TGTCACAGAA GCAGGCAGCC TGTAAGAAAG 
TTAGAGCCCA TCCCACCTCA GGTCCTTCAA AAGCTGCAGG ATAGAGAACG TGCTCAGCTA 
GCTCAGCTAG AGCAAGAGAT GAGAGAGCGA GACCAGGCCA AAGAACGATC ACCTCCGAAA 
TCACCGTCTC GCAGAAGGAA AGACCATCTG GCACAACAAC CACCAGAAAG AGCTGAGACC 
AAGGTGGTAA GCACAACTGG ACTTGAACCA TCCCCTCCAG AAGCCATGTC ACTCTCTGAT 
TTACCAGGTC AAAGATCTCC AAGATTTCGG GGCCCAAGCC CAAGCAGAGA TAGTACACGA 
AGGTCTCCAA CCGTAGAGAT TAGTGCCATG GCTGAGGAGC GCCCTCGAGG AAGAGCTGAA 
TCTCCTTCAA GAAATGTTTT GGAGATGACG TTACGCAAAG TGGAGCCAAG ACCTGCAAGT 
CCACTGGTAA AGAGGGTGGT TCGTGTTCAA GAAGTTCCAC AAGCAAACGA TCAGCCTATG 
CATAGAAAGA CGCCAATAGA GGTCTCGCTA AGAAAACTGG AAAGAAGGCC TGAAAGTCCC 
CTGGTGCAAG GAGAAACCGT AGCCATTCAA GACTTCCCTG TTCAAGCTCC CCCCAAACCT 
CCAAGAGTTT CCTCATCTTC CTCGTCTGAG GAGAAGATGG AGCTGGAAGT GACTCCTCCA 
CCACCCCCAC TAAAACTCAC TATACCAAAA ATTATTGTTG AAGAAGAGCC CATGGAGACA 
GACACACCTG TTGAGAAGAC TGAAAAGGCT GTGAAAGTTG GCACTGGTAA AGATGAAAAA 
CATCAAAGAT CCAGAGGAAG AGGACGCAGA CAAAGACCAA TGTCCCCTGA ATTAGAATCT 
TCGGACGACT CCTATGTGTC TGCAGGAGAG GATCCACTAG AAGCTCCTGT GTTCGAGTTT 
CCACTTCAGG ACACTGTGGC CTCCACTGGA ACGGAGGTGC TATTAAAATG CATCATCAGT 
GGCACCCCTC TCCCTGAAGT TATATGGAAG AAAGACAACA CTGAGGTCAA GAACAGTCCC 
ACTCATGTTG TAAAGATAGA GGGGGAACGC CACTCCTTGC TCATCAAATG GACAAAGCCA 
AGTGATGCAG GAACCTACAC AGTTACCGCT GTCAATGAGG TCGGTGAGGT GTCCAGCAGT 
GCTACACTTT TCATCAAACC AGAGCCCAGT CAGGACCATC GTGGTAACCT TGGTGTCCCT 
ATGGATGTCA GCAGTCCCAT CACATCTGAT GAGGAGTATC TCAGTCCACT GGAAGAGAGC 
ATGGACTTCT CCTCCTACAG GGGACCGGAG CCAAGAAAGA TCGTGGACAC TCGATTTAAG 
GAGCCTCCAG CCTTTCTGGC TGTTATTGGA GACCAAAGCG TAATAGAGGG TCAGGAGGTG 
ACCATGTCTG TTCTAGTGAC CGGACAACCT AAACCTATGC TTTACTGGCT GAGAGACAGA 
GTGACCATTA AAACCGGAAC ACGGCATATC GTCCAAGAGA CAGAGGAGGG CAACTTTGAA 
ATGATAATTA AATCAGCACA GAGGTCAGAC ACCGGTGTTT ACACCTGCAA GATCATCAAT 
GAATATGGAA CTAAGCAATG TGAAGGCAAA CTGGAGGTGA AAGCTCGTCC AGTAGAGCCA 
GGTCTGGCCA TCATTCGGCC AGTGAGGGAC GTGATGGTGA AGGCAGGGGA GACCGCTCTG 
TTTGAGTGTC ATGTGATCGG CCCGCAGGAC ACAGATGTTG ACTGGCTGTC AGATGGGAAA 
CTCATCCAGC CTGCTTTGTT GAACTGCAAG ATGCATTTTG ATGGGAAAAG GTGCAGGCTG 
CTTCTAAACT CTGTACATGA GGATGACAGT GGAACGTACA CCTGCAAACT GAGCACAGCT 
AAAGAGGAAC TGACCTCCAG TGCCAAGCTA AAAGTCATAG CATCCATAGA GCCCCTTTTC 
ACTCGTAAGC TGGATGTGCT GGAAGTTATC GAGGGTCGTA ATGCACGATT TGACTGCAAG 
GTCAGCGGGA CGCCGCCGCC TCAAGTCATA TGGAGCCACT TCGATCGTCC GCTCGAGGAA 
AACGAAGATA TCAGGATCTT AAAAGAAGGA GGACGGCACT CATTAATCAT CTCTCATGTT 
AGTAATGAAG ATGAGGGCCT CTACACGGTT GCAGCGCGAA ACTCACACGG GGAAGACGAA 
TGTGCAGCGG AGCTCTATGT GCAGGAGCCC AGACCAGCAA TATCTTCTCA AATAGCGAAG 
CTGGAAAAAA TGCCTTCCAT TCCCGAAGAA CCCGAAGTTC CTGAAAATGA GATTGAGCGT 
TTCACCATGC CTGACTTCGT AAAGCCGCTT TATGATATGG ATGTAGTGGA GGGCAGAGAG 
GCTGTGCTCA GGTGCAAAGT GGCCGGCTTG CCGTACCCCA CCATAACATG GTTTCACAAT 
GGCAAGAGAA TTGACAGCAC GGAGGACAGA AAGATGACAC AGTTCAGAGA TGTTCACAGC 
CTGGTGGTCC GCTGTGTTTG TCATGCTCAC GGAGGGGTTT ACAAGTGTGT CATATCCAAT 
AAAGTGGGCA AGGCCACCTG CTATTCTCAT CTTTACGTGA CAGACATCCT TCCTGACCCT 
CCAGATGGTG CCCCAGTCGT TGAGTCCATC ACTGGCAAGA CTATCACGCT AAGCTGGAAG 
AAACCCAAAA GACTGGATCC GTCCATTGAC CCCAGCTCCC TGATGTATGC CATTCAGCAA 
CAGGCCCTGG GGTCGATCCA GTGGACCATC ATCGCTTCTT GCCTGAAACA GACCACCTAT 
ACCATCAGCA ATCTGTCTAA AGGTGTCCGC TACGCCTTCA GGGTGCTATC AATCACCAGC 
AAGGCCTTTA GCAAGCCGTC GCCTGCCACC GAGCCAATGC AGCTGCTAGA TAGAGGTCCG 
TACGTCCAGG AGGCTCCGGT AATCATTGAC AAGCCAGATA TCGTGTATAT GGTGGAGAAG 
CAGCCCTTGA GCATCACAGT CACTCTCAAC CACGTCAACG CATCGGTCAC ATGGAAGAGA 
GGAGGAGTGA CATTATCTAA CCGACCGGGT CTTTTTGAGT TGAGCATGCC TGATGATGAC 
CAGCACACAC TGAAGCTGTG TAAGGTGAAG AGCAGTGATG TTGGCCAGCT CACCTGCATT 
GCCAACAACA AGTACGGCAG CGACTCCTGT GTCCTCACCC TGGAGATGGC AGTGGCACCA 
ACTTTTGAGA CAATTATGGA AGACCTGGAT GTGAATGTAG GCGAGACTCC TCGATTTGCA 
GTGGTGGTTG AAGGAAAGCC CGTCCCAGAC ATCCTCTGGT ACAAGGGAGA CACCCTTCTG 
TCCGAAAGCA GCCATTTCAC ATTTGTGTAT GATGACAATG AATGTTCTCT AGTGGTGCTC 
AACACTCAAG CAGACGACTC AGGAGTGTAC ACTTGTACTG CGAAGAATCT GGCCGGTTCT 
GTGTCCTGCA AGGCTGAGCT GACCGTCCAC ACCACCAAAC AGTACAAAGA CGACCCGATG 
GAGGATGAGG CGTCCATCCT CAGAAAGATG CGGAGGCTCA CTGATTACTA TGATGTGCAC 
AAGGAAATTG GGAGAGGGGC TTTCTCATAT GTGAAGCGGG TGATACAGAA GGCAGGAAAG 
CTGGAATACG CAGCCAAGTT TATTTCTGCT CGGGCTAAAC GAAAGGCATC CGCTCTGAGA 
GAACTGAACA TCCTCTCTCA TCTGGACCAT GAGAGGATCC TCTATTTTCA TGATGCCTTT 
GAGAAGAAGA ATGCTGTCAT CATCATTACA GAGCTATGCC ATGAGGAGCT TCTCGATAGG 
CTTACAAAGA AATCAACCAT CTTGGAGTCC GAGATCCGAT CCAGCGTGAG GCAGCTGTTG 
GAGGGTATTA ACTATCTCCA TCAGCTTGAC ATACTTCATC TAGACATCAA GCCTGATAAT 
ATCCTCATGG CAGACCACAG TAGCGATCAG ATCCGCATAT GTGATTTTGG AAATGCTGTA 
AAGTTCATGC CTGATGAGGC ACAGTACTGC AAATATGGCA CTCCAGAATT TGTCGCCCCA 
GAGATTGTTA ACCAGACACC CGTCTCCAAG GCAACGGACA TCTGGCCCAT TGGTGTTCTG 
ACATACTTAT GTCTTACTGG AGTTTCTCCT TTTGCTGGGG AAAACGACCG CAGCTCTGTT 
CTCAACATCA GGAATTATAA TGTGGCTTTT GAAGAAAGCA TGTTTACAGA CCTCTGCCAT 
GAAGCCAAGG GATTCGTCAT CAAGCTGCTG GTGGCTGATA GATTGAGGCC TGATGCTAAT 
GAATGTCTTC GCCACCCCTG GTTCAAGACC CTCAACAAGG GTAAAAGCAT CAGTACAGAG 
TCCCTTAAAA AGTTTTTGTC CAGAAGAAAA TGGCAGCGAT CGCTTATCAG CTACAAATCT 
AAAATGGTGA TGAGGTCTGT ACCTGAGCTA CTGGATGACT CCTCCAGCCA TATTTCCATT 
GCTGTTCCGC GACATCTGAA AAAAGGCTCA CCACCACTTT CATCATCTTC AGATTCAGAT 
GAAGACATCG ATGAGTTGCC TTTTATTCCC ATGCCACTCA ACGTGGAATT CTCAGGATCT 
AGAATGTCAC TGACCGAAAT TGTAGGTGAT GATGACATGA CAGGTAAATT GAATGGAAAT 
GCTGAGAGGC CTGCTTCACT CCATCCGAAG TCCGAGCCAA TGGAAGTGGA GATGTCAGAG 
AGAGAAGGTG GAGATCCATC TGCTAGGGGT AGAGCCAAGA AGAGGACTAC AACAGAAGAT 
GAAAAAGGGT CGTCTGATGA GGAACCATCA CAACTGGCCA AAAGAGCAGA ACTGGCAAAG 
AGACCAATGC GCAGAGGTTC AAGCATGGAG TCAGATGAGC CAGAGGGAGG ACGTCGCAGA 
GGAGAGTTAC GCAGAGGGAG TTCGGCTGAC AGTGCCTTAC AGCTCCATAT CAAACCAGAG 
GAGGGCACAG AAGAAAGTCC TACTGATGGA AGTAGAATTC TACAAAAGTC TGTATCTATG 
GAGCTTCCAA GGAGAAGCCC AAGTCCAGGT GCTGCAAAGC TTAGCCAGGA AGACTATGCT 
CTGAAACTGG AACTGATGAG GCAGCGACTA CTCAGGGGTG GCTCTGCTGA CAACAAGATG 
AGTGGATTAC GAGGCCCATT GCTGGAGACA CTAGGTATGG GAGATGAGAA GCGGTTTGTG 
CGGGGATCTC GTCTAGGTAA CCCTCCACTT GTTAGAGCGG CATCTAGTGA GTCACCTAGA 
GATGACATCC CAAAGACAAA GATTCTCCGC AAGAGTGCTT CCTTTAGCCA GGGGGATTCA 
GAGCCCATGC CCCTGCACAG AAGAATCGGA GCACCTCTAG AGATTCCTCT AGCACAAATA 
GAGGAGAGAC GCCTACAGGA AGCAATATCC ATGTCTGCTC TCACAGAGCC TGTCAAAGAT 
TCTCGCCCAA TCACTCCAAA ACTTCAAATG CCTGAAGAAG AAAGACATGA AAAGAAGAAA 
ACTGAAGAAA AAGAATCTGT CTACAAGGAC AATAAACCTG AAGAGGTTAT TGCTGTGTCA 
AAGACTGAGG AAGAAGGAAG ACGTGTTGAT CAGAAATCTT GGGATTCTGA TGCACAGAAA 
AAGGACCAGA GCAAGTCAGA GGAAACCAGG GAAGAGTCTG AAAAAGTGGC TGAAATTGAA 
AAAAAGACCT CTATAGTGGA CGAAACAATT GAGGAAGAGG AAACAAGAAA AGATGAGGAG 
CCAGAGAAAG AAACTGAGCC TCCTACTCCA AAGATTGAGG TTAAGGAATA TGAGGGTGAT 
GTAAAGGTAT TACCAAAGTC TCCAGAGTTT ATCATTTCCA CAAACTCAAT CATGGTGACC 
CCAACAGCTG CCCCAGCATC CAGTCCCCAG AGTGTATCAA CCTATGTTAC CCCATCTCCC 
CCTGCACGCA CAGTGCTCCC AGATGGTAGG ATGTCCGCAT ATGCCAGTAT TATGCAGACT 
ATCATGGTAC CATCTGTCCC AGCAAATACC CAATCTGACA CTGTTCCTAT TCCTACACCA 
ACTTCCACTC CACCATCAGT TCCAGTCTAT GCACCCATTT CCAGACACAT CTCAGAACCA 
ACCCTTTCAT CAACTTCTTC ATCCAAATCA GATCTTCACC AAACAACTGA GCATCCTGCA 
GTCTTTTCTC GGGTTGCCTC CACTGAACAC CCTCCCAAAG AGCCAAGTCC TCCTAAGACA 
CCCACTCGCG CCTCCCCTAA GCGAGAGCCA TCTCCTGGAA GCAACATCCA GGACCTTGCA 
TCCGAGGAGG TTTTTGAAGC TCGCTTCAAG AAACGTGAGT CTTCTCTAAC TCGTAGTCTC 
AAACGTTTCT CATTTCAGAA GACTGAAGAC AAGCCCTCCA CTGTTTCTCC TGAAACTGCT 
GAAGAAATGT ATCGCCCTGG ACCAACTGGT GCTCCTTTAG AGTTTGTGCC AAGGAGGTTG 
GAGGAGAAGT CCAAATCAGT ACAAGATCTT CGAGAGGCAG AGAAGGACCC TGGGTTCATG 
AGGCGACTCT CCATGCGCTT GAAAAGGACC CCATCAGTGG AAAAGCAGGA GGAGAAGCTC 
AAAGAGGAGG AGGCTTCAGC ACCCAGGCGT CGTCTTTCTT GGGCACTGGG ACGGAGGGGA 
TCCCAAGAGA AGAAGGAGAT TGAGATGATG AGGTTGGATG GGGGTCCTGA GCCTCCCCCA 
GAGCCTGAGT CTAAAGCACC TTCTGAGTCA CCTATTCTTG CCATGCGCAA GAAGATTGGC 
AGCACCATGG CAAGCTTATC TACAAGAATT AGGAGCCATT CTGAGGACAG GAAAGATGAC 
AGTGAAGGCA AGATGGAGAC CAAGAGGACT CCTATATTCA ACAAACTAAG GCGTTCCACT 
TCTGAGGGGG GCAGTCTGAA GAGAATGGGT ATCCCACAAA ACCAACTGGC TGCTCAGTCA 
GGGCAAGGGA CCTCATCAGA ATCTCTTGAT TCTATGTCAA GCATCCAGTC AGAATCAGCT 
ATTAAAAATG CAGAACTTGA CCGCAGATCA CGGTGGGACC GATGGGGCTT GACTCGAGGG 
AAAAAGGACA AGACAGTTTC CCAACCAGAC ATCCCAGCTT CCATTGCTAG AGAGAATGGT 
TCTCTACGGT CTCGCCAATA TACACGTCTA ACATCTGACT TCCCTCCGGT GTTCCATATC 
AAACTGAGGG ACCATGTACT CTTGGAGGGA GATCCTGTCA CTCTCAGCTG CCTCCCTGCT 
GGCAGTCCTC ACCCACACAT CTCATGGATG AAAGACAAGA AGCCACTTGA GATTGACCCC 
AGGATGAATA TGATATCTTG CCCCGATGGG AGACAACTGC TGATGATTAT GAAGACCACC 
AAGAAGGATG CAGGCCTCTA TGAATGTGTG GCTGCAAACC CACTGGCCAC AGTCACCAGC 
TCATGCGTTG TGTCCCTCGC TCGTCTGCCT AATAGTCCTG GGACACCCGA GGTTCCTCAG 
AAGTATAAAA ACACTGCTCT AGTGGTATGG AGACCCTCAG ACACTACGGC ACCCTGCACC 
TACTCTCTGG AGAGGAAGAC AGAGGGTGAG AATAACTGGC TGATAGTCGC CACAGGTGTT 
GCTGACTGTT ACTATAACGT GTTGGATCTT CCGGCCGGCG CTTCATACAG GTTCAGAGTG 
GCCTGCGTCA ATAAGGCTGG TCAGGGACCT TACAGCAGCT TGTCTGAAGT AGTGGTCCTG 
GACTCAACAG AGCCCCAGAA GTCCAGTGGC ACAGTTGTAG TCAAAACGCT TTCATCAGCT 
CCCGCTCCTG CAGTGGTAAC GTCCACCATG ACCCTTCCTG CAATGAAGCC TGCTGCAATA 
AAACCAGCAC CATCAACAAA TTCAGCGCCC TCTACAGTCC AACCGGTAAA GCCTGCGCCT 
CCTCCTGCAT CATCAACACC TACCTATCGT CCCGTGCCTG ACATTCGAAC TCCATCGCCT 
GGCACTCCAA CCCCTGCCAA AGCCAAAAGC ACCCTCAACA TTACTATGGC CAAACCCTCC 
CCAGCATCCC CTGCAGCTCA ACCACAACCA CAACCTCCAC CCAAACCCTC GCCTCCGGTC 
ATTCTGCCCA AACCTCAGAC CCCAGTCAGT GTGGTTTCAC CTCCATCCCA AACTCCTCCA 
GTGTCACCTC CACCTCTAGT GTCTCCTCCT CCCATTATTG GTAAACCAAT CTCCTCAGTG 
CCCATGTATG TCCCTGCTAC AACCACCTCG ACTCCAGTGA CTCCGGTCAC TCCTGTCACC 
CCAGTCACTC CAGTCACCCC AAATACGCCC TCTCCTGCCC TGTCGCCTCC AGTGGTGTTG 
GTTACAAGTC TGAGTCCTGT AGGGGAGGGA ACTGGGACAC CCAATCGAAT GACCCCTAGT 
GGAAGAGCCA CGCCTTCTGG ACGCATCACT CCATCAGGCC GAAGGACCCC TATTGGAAAG 
CCTGGAGACT CAACGCTGAG GCAAGGAGTC CCTCAGAAAC CTTACACCTT CATGGATGAG 
AAAGCAAGGG GTCGCTTTGG TGTGATCAGA GAGTGTCGAG AAAATGCCAC TGGTAACCTG 
TACATGGCTA AGATTGTGCC ATATGAGCCA GAGAGCAAGC AGACTGTATT ACAGGAATAC 
GACATCCTGA AGTCTCTCCA TCATGAGAAG ATCATGGCTC TCCACGAGGC CTACGTCACT 
CCACGCTACC TGGTGCTCAT CTCTGAGTGC TGCTCTGGCA AAGAACTACT CCACAGTCTC 
ATTGACAGGT TCCGATACTC TGAGGATGAT GTGGTTGCAT ATATCGTACA GATCCTTCAG 
GGTCTGGACT ACCTGCACAG CCGGAGGATC CTGCATCTCG ACATCAAACC AGAAAACATC 
ATCGTCACTT ACATGAATGT GGTGAAGATT ATCGATTTTG GTAGTGCACA GACCTTCAAT 
CCTCTGTTCC TGAAGCAGTT CTCCCCACCT ATTGGAACAC TGGATTATAT GTCTCCAGAG 
ATGCTGAAAG GAGATGTGGT GGGTCCACCA GCTGACATCT GGAGCATTGG CATATTGACT 
TATATCATGC TTAGCGGGAG GTTGCCGTTC ACAGAAAATG ACCCTGCCGA GACCGAGGCC 
AGAATACAGG CAGCCAAGTT TGACCTCAGT AAGCTTTACC AAAACGTGTC CCAAAGTGCC 
TCTCTGTTCA TCAAAAAGAT CCTGTGCAGC TACCCTTGGG CCCGTCCGAC CATCAAGGAC 
TGCTTTACCA ACTCGTGGCT GCAGGACGCA TATCTGATGC GCCTGCGAAG ACAGACTCTC 
ACATTCACCA CCACACGCCT CAAAGAGTTC CTGGAGCAGC AGCAGCACAT CAGAGCCCAA 
GAGGCAACAA AGCACAAAGT CCTCCTGCGT TCCTACCAGA GCAACCCACA AACCCCATCC 
ACCCCTCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spegb ( XM_009304895.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009303170.1 Danio rerio SPEG complex locus b (spegb), transcript variant X1, mRNA.

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CloneID	ODa43992
Clone ID Related Accession (Same CDS sequence)	XM_009304895.2 , XM_009304896.2
Accession Version	XM_009304895.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9879bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	striated muscle preferentially expressed protein kinase
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304895.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGA GTAGTGCTCA AGACTCTCGA CCCCAAACCC CTGTCAGTGA GTATTCCCGT 
AAGGAGCTTA CACTTCGGCC CTCACCAAAA CTGACAAGAG CTAGCTCCAA AATCTTTGAG 
AAGGTGCGAG TCTTCGAAGA GCGCAGAAGA AGCATTGATA ACCCTGAAGG ATCTATATCT 
GGACGTTCTT GGGCTGGATT TAACAGAGCT TCATCTATCG ACTCTGATGA AGGAGGGAGC 
AGGTTGGGCA TCTCCAGGGA GAGCTCCAAA GAAGACCTTC GTGAGGCTTT GAAGGCAGAT 
GCAGCCCAAC GAAGGACCAT GTTTAAACAG CGGGCTGCTT CCCTGGAGGA CAGACCTCGT 
TACACCCAGA AGGTGCAGGA CATTGAAAAC AAGTTCACAG AGGAGCTCCA GCGCATCAAA 
AAGCTTGTCG GCAAACCACA CATGAAGAAA TCCTTCTCAA CAGAGCAGCT CTCTACTTGT 
CACAGAAGCA GGCAGCCTGT AAGAAAGTTA GAGCCCATCC CACCTCAGGT CCTTCAAAAG 
CTGCAGGATA GAGAACGTGC TCAGCTAGCT CAGCTAGAGC AAGAGATGAG AGAGCGAGAC 
CAGGCCAAAG AACGATCACC TCCGAAATCA CCGTCTCGCA GAAGGAAAGA CCATCTGGCA 
CAACAACCAC CAGAAAGAGC TGAGACCAAG GTGGTAAGCA CAACTGGACT TGAACCATCC 
CCTCCAGAAG CCATGTCACT CTCTGATTTA CCAGGTCAAA GATCTCCAAG ATTTCGGGGC 
CCAAGCCCAA GCAGAGATAG TACACGAAGG TCTCCAACCG TAGAGATTAG TGCCATGGCT 
GAGGAGCGCC CTCGAGGAAG AGCTGAATCT CCTTCAAGAA ATGTTTTGGA GATGACGTTA 
CGCAAAGTGG AGCCAAGACC TGCAAGTCCA CTGGTAAAGA GGGTGGTTCG TGTTCAAGAA 
GTTCCACAAG CAAACGATCA GCCTATGCAT AGAAAGACGC CAATAGAGGT CTCGCTAAGA 
AAACTGGAAA GAAGGCCTGA AAGTCCCCTG GTGCAAGGAG AAACCGTAGC CATTCAAGAC 
TTCCCTGTTC AAGCTCCCCC CAAACCTCCA AGAGTTTCCT CATCTTCCTC GTCTGAGGAG 
AAGATGGAGC TGGAAGTGAC TCCTCCACCA CCCCCACTAA AACTCACTAT ACCAAAAATT 
ATTGTTGAAG AAGAGCCCAT GGAGACAGAC ACACCTGTTG AGAAGACTGA AAAGGCTGTG 
AAAGTTGGCA CTGGTAAAGA TGAAAAACAT CAAAGATCCA GAGGAAGAGG ACGCAGACAA 
AGACCAATGT CCCCTGAATT AGAATCTTCG GACGACTCCT ATGTGTCTGC AGGAGAGGAT 
CCACTAGAAG CTCCTGTGTT CGAGTTTCCA CTTCAGGACA CTGTGGCCTC CACTGGAACG 
GAGGTGCTAT TAAAATGCAT CATCAGTGGC ACCCCTCTCC CTGAAGTTAT ATGGAAGAAA 
GACAACACTG AGGTCAAGAA CAGTCCCACT CATGTTGTAA AGATAGAGGG GGAACGCCAC 
TCCTTGCTCA TCAAATGGAC AAAGCCAAGT GATGCAGGAA CCTACACAGT TACCGCTGTC 
AATGAGGTCG GTGAGGTGTC CAGCAGTGCT ACACTTTTCA TCAAACCAGA GCCCAGTCAG 
GACCATCGTG GTAACCTTGG TGTCCCTATG GATGTCAGCA GTCCCATCAC ATCTGATGAG 
GAGTATCTCA GTCCACTGGA AGAGAGCATG GACTTCTCCT CCTACAGGGG ACCGGAGCCA 
AGAAAGATCG TGGACACTCG ATTTAAGGAG CCTCCAGCCT TTCTGGCTGT TATTGGAGAC 
CAAAGCGTAA TAGAGGGTCA GGAGGTGACC ATGTCTGTTC TAGTGACCGG ACAACCTAAA 
CCTATGCTTT ACTGGCTGAG AGACAGAGTG ACCATTAAAA CCGGAACACG GCATATCGTC 
CAAGAGACAG AGGAGGGCAA CTTTGAAATG ATAATTAAAT CAGCACAGAG GTCAGACACC 
GGTGTTTACA CCTGCAAGAT CATCAATGAA TATGGAACTA AGCAATGTGA AGGCAAACTG 
GAGGTGAAAG CTCGTCCAGT AGAGCCAGGT CTGGCCATCA TTCGGCCAGT GAGGGACGTG 
ATGGTGAAGG CAGGGGAGAC CGCTCTGTTT GAGTGTCATG TGATCGGCCC GCAGGACACA 
GATGTTGACT GGCTGTCAGA TGGGAAACTC ATCCAGCCTG CTTTGTTGAA CTGCAAGATG 
CATTTTGATG GGAAAAGGTG CAGGCTGCTT CTAAACTCTG TACATGAGGA TGACAGTGGA 
ACGTACACCT GCAAACTGAG CACAGCTAAA GAGGAACTGA CCTCCAGTGC CAAGCTAAAA 
GTCATAGCAT CCATAGAGCC CCTTTTCACT CGTAAGCTGG ATGTGCTGGA AGTTATCGAG 
GGTCGTAATG CACGATTTGA CTGCAAGGTC AGCGGGACGC CGCCGCCTCA AGTCATATGG 
AGCCACTTCG ATCGTCCGCT CGAGGAAAAC GAAGATATCA GGATCTTAAA AGAAGGAGGA 
CGGCACTCAT TAATCATCTC TCATGTTAGT AATGAAGATG AGGGCCTCTA CACGGTTGCA 
GCGCGAAACT CACACGGGGA AGACGAATGT GCAGCGGAGC TCTATGTGCA GGAGCCCAGA 
CCAGCAATAT CTTCTCAAAT AGCGAAGCTG GAAAAAATGC CTTCCATTCC CGAAGAACCC 
GAAGTTCCTG AAAATGAGAT TGAGCGTTTC ACCATGCCTG ACTTCGTAAA GCCGCTTTAT 
GATATGGATG TAGTGGAGGG CAGAGAGGCT GTGCTCAGGT GCAAAGTGGC CGGCTTGCCG 
TACCCCACCA TAACATGGTT TCACAATGGC AAGAGAATTG ACAGCACGGA GGACAGAAAG 
ATGACACAGT TCAGAGATGT TCACAGCCTG GTGGTCCGCT GTGTTTGTCA TGCTCACGGA 
GGGGTTTACA AGTGTGTCAT ATCCAATAAA GTGGGCAAGG CCACCTGCTA TTCTCATCTT 
TACGTGACAG ACATCCTTCC TGACCCTCCA GATGGTGCCC CAGTCGTTGA GTCCATCACT 
GGCAAGACTA TCACGCTAAG CTGGAAGAAA CCCAAAAGAC TGGATCCGTC CATTGACCCC 
AGCTCCCTGA TGTATGCCAT TCAGCAACAG GCCCTGGGGT CGATCCAGTG GACCATCATC 
GCTTCTTGCC TGAAACAGAC CACCTATACC ATCAGCAATC TGTCTAAAGG TGTCCGCTAC 
GCCTTCAGGG TGCTATCAAT CACCAGCAAG GCCTTTAGCA AGCCGTCGCC TGCCACCGAG 
CCAATGCAGC TGCTAGATAG AGGTCCGTAC GTCCAGGAGG CTCCGGTAAT CATTGACAAG 
CCAGATATCG TGTATATGGT GGAGAAGCAG CCCTTGAGCA TCACAGTCAC TCTCAACCAC 
GTCAACGCAT CGGTCACATG GAAGAGAGGA GGAGTGACAT TATCTAACCG ACCGGGTCTT 
TTTGAGTTGA GCATGCCTGA TGATGACCAG CACACACTGA AGCTGTGTAA GGTGAAGAGC 
AGTGATGTTG GCCAGCTCAC CTGCATTGCC AACAACAAGT ACGGCAGCGA CTCCTGTGTC 
CTCACCCTGG AGATGGCAGT GGCACCAACT TTTGAGACAA TTATGGAAGA CCTGGATGTG 
AATGTAGGCG AGACTCCTCG ATTTGCAGTG GTGGTTGAAG GAAAGCCCGT CCCAGACATC 
CTCTGGTACA AGGGAGACAC CCTTCTGTCC GAAAGCAGCC ATTTCACATT TGTGTATGAT 
GACAATGAAT GTTCTCTAGT GGTGCTCAAC ACTCAAGCAG ACGACTCAGG AGTGTACACT 
TGTACTGCGA AGAATCTGGC CGGTTCTGTG TCCTGCAAGG CTGAGCTGAC CGTCCACACC 
ACCAAACAGT ACAAAGACGA CCCGATGGAG GATGAGGCGT CCATCCTCAG AAAGATGCGG 
AGGCTCACTG ATTACTATGA TGTGCACAAG GAAATTGGGA GAGGGGCTTT CTCATATGTG 
AAGCGGGTGA TACAGAAGGC AGGAAAGCTG GAATACGCAG CCAAGTTTAT TTCTGCTCGG 
GCTAAACGAA AGGCATCCGC TCTGAGAGAA CTGAACATCC TCTCTCATCT GGACCATGAG 
AGGATCCTCT ATTTTCATGA TGCCTTTGAG AAGAAGAATG CTGTCATCAT CATTACAGAG 
CTATGCCATG AGGAGCTTCT CGATAGGCTT ACAAAGAAAT CAACCATCTT GGAGTCCGAG 
ATCCGATCCA GCGTGAGGCA GCTGTTGGAG GGTATTAACT ATCTCCATCA GCTTGACATA 
CTTCATCTAG ACATCAAGCC TGATAATATC CTCATGGCAG ACCACAGTAG CGATCAGATC 
CGCATATGTG ATTTTGGAAA TGCTGTAAAG TTCATGCCTG ATGAGGCACA GTACTGCAAA 
TATGGCACTC CAGAATTTGT CGCCCCAGAG ATTGTTAACC AGACACCCGT CTCCAAGGCA 
ACGGACATCT GGCCCATTGG TGTTCTGACA TACTTATGTC TTACTGGAGT TTCTCCTTTT 
GCTGGGGAAA ACGACCGCAG CTCTGTTCTC AACATCAGGA ATTATAATGT GGCTTTTGAA 
GAAAGCATGT TTACAGACCT CTGCCATGAA GCCAAGGGAT TCGTCATCAA GCTGCTGGTG 
GCTGATAGAT TGAGGCCTGA TGCTAATGAA TGTCTTCGCC ACCCCTGGTT CAAGACCCTC 
AACAAGGGTA AAAGCATCAG TACAGAGTCC CTTAAAAAGT TTTTGTCCAG AAGAAAATGG 
CAGCGATCGC TTATCAGCTA CAAATCTAAA ATGGTGATGA GGTCTGTACC TGAGCTACTG 
GATGACTCCT CCAGCCATAT TTCCATTGCT GTTCCGCGAC ATCTGAAAAA AGGCTCACCA 
CCACTTTCAT CATCTTCAGA TTCAGATGAA GACATCGATG AGTTGCCTTT TATTCCCATG 
CCACTCAACG TGGAATTCTC AGGATCTAGA ATGTCACTGA CCGAAATTGT AGGTGATGAT 
GACATGACAG GTAAATTGAA TGGAAATGCT GAGAGGCCTG CTTCACTCCA TCCGAAGTCC 
GAGCCAATGG AAGTGGAGAT GTCAGAGAGA GAAGGTGGAG ATCCATCTGC TAGGGGTAGA 
GCCAAGAAGA GGACTACAAC AGAAGATGAA AAAGGGTCGT CTGATGAGGA ACCATCACAA 
CTGGCCAAAA GAGCAGAACT GGCAAAGAGA CCAATGCGCA GAGGTTCAAG CATGGAGTCA 
GATGAGCCAG AGGGAGGACG TCGCAGAGGA GAGTTACGCA GAGGGAGTTC GGCTGACAGT 
GCCTTACAGC TCCATATCAA ACCAGAGGAG GGCACAGAAG AAAGTCCTAC TGATGGAAGT 
AGAATTCTAC AAAAGTCTGT ATCTATGGAG CTTCCAAGGA GAAGCCCAAG TCCAGGTGCT 
GCAAAGCTTA GCCAGGAAGA CTATGCTCTG AAACTGGAAC TGATGAGGCA GCGACTACTC 
AGGGGTGGCT CTGCTGACAA CAAGATGAGT GGATTACGAG GCCCATTGCT GGAGACACTA 
GGTATGGGAG ATGAGAAGCG GTTTGTGCGG GGATCTCGTC TAGGTAACCC TCCACTTGTT 
AGAGCGGCAT CTAGTGAGTC ACCTAGAGAT GACATCCCAA AGACAAAGAT TCTCCGCAAG 
AGTGCTTCCT TTAGCCAGGG GGATTCAGAG CCCATGCCCC TGCACAGAAG AATCGGAGCA 
CCTCTAGAGA TTCCTCTAGC ACAAATAGAG GAGAGACGCC TACAGGAAGC AATATCCATG 
TCTGCTCTCA CAGAGCCTGT CAAAGATTCT CGCCCAATCA CTCCAAAACT TCAAATGCCT 
GAAGAAGAAA GACATGAAAA GAAGAAAACT GAAGAAAAAG AATCTGTCTA CAAGGACAAT 
AAACCTGAAG AGGTTATTGC TGTGTCAAAG ACTGAGGAAG AAGGAAGACG TGTTGATCAG 
AAATCTTGGG ATTCTGATGC ACAGAAAAAG GACCAGAGCA AGTCAGAGGA AACCAGGGAA 
GAGTCTGAAA AAGTGGCTGA AATTGAAAAA AAGACCTCTA TAGTGGACGA AACAATTGAG 
GAAGAGGAAA CAAGAAAAGA TGAGGAGCCA GAGAAAGAAA CTGAGCCTCC TACTCCAAAG 
ATTGAGGTTA AGGAATATGA GGGTGATGTA AAGGTATTAC CAAAGTCTCC AGAGTTTATC 
ATTTCCACAA ACTCAATCAT GGTGACCCCA ACAGCTGCCC CAGCATCCAG TCCCCAGAGT 
GTATCAACCT ATGTTACCCC ATCTCCCCCT GCACGCACAG TGCTCCCAGA TGGTAGGATG 
TCCGCATATG CCAGTATTAT GCAGACTATC ATGGTACCAT CTGTCCCAGC AAATACCCAA 
TCTGACACTG TTCCTATTCC TACACCAACT TCCACTCCAC CATCAGTTCC AGTCTATGCA 
CCCATTTCCA GACACATCTC AGAACCAACC CTTTCATCAA CTTCTTCATC CAAATCAGAT 
CTTCACCAAA CAACTGAGCA TCCTGCAGTC TTTTCTCGGG TTGCCTCCAC TGAACACCCT 
CCCAAAGAGC CAAGTCCTCC TAAGACACCC ACTCGCGCCT CCCCTAAGCG AGAGCCATCT 
CCTGGAAGCA ACATCCAGGA CCTTGCATCC GAGGAGGTTT TTGAAGCTCG CTTCAAGAAA 
CGTGAGTCTT CTCTAACTCG TAGTCTCAAA CGTTTCTCAT TTCAGAAGAC TGAAGACAAG 
CCCTCCACTG TTTCTCCTGA AACTGCTGAA GAAATGTATC GCCCTGGACC AACTGGTGCT 
CCTTTAGAGT TTGTGCCAAG GAGGTTGGAG GAGAAGTCCA AATCAGTACA AGATCTTCGA 
GAGGCAGAGA AGGACCCTGG GTTCATGAGG CGACTCTCCA TGCGCTTGAA AAGGACCCCA 
TCAGTGGAAA AGCAGGAGGA GAAGCTCAAA GAGGAGGAGG CTTCAGCACC CAGGCGTCGT 
CTTTCTTGGG CACTGGGACG GAGGGGATCC CAAGAGAAGA AGGAGATTGA GATGATGAGG 
TTGGATGGGG GTCCTGAGCC TCCCCCAGAG CCTGAGTCTA AAGCACCTTC TGAGTCACCT 
ATTCTTGCCA TGCGCAAGAA GATTGGCAGC ACCATGGCAA GCTTATCTAC AAGAATTAGG 
AGCCATTCTG AGGACAGGAA AGATGACAGT GAAGGCAAGA TGGAGACCAA GAGGACTCCT 
ATATTCAACA AACTAAGGCG TTCCACTTCT GAGGGGGGCA GTCTGAAGAG AATGGGTATC 
CCACAAAACC AACTGGCTGC TCAGTCAGGG CAAGGGACCT CATCAGAATC TCTTGATTCT 
ATGTCAAGCA TCCAGTCAGA ATCAGCTATT AAAAATGCAG AACTTGACCG CAGATCACGG 
TGGGACCGAT GGGGCTTGAC TCGAGGGAAA AAGGACAAGA CAGTTTCCCA ACCAGACATC 
CCAGCTTCCA TTGCTAGAGA GAATGGTTCT CTACGGTCTC GCCAATATAC ACGTCTAACA 
TCTGACTTCC CTCCGGTGTT CCATATCAAA CTGAGGGACC ATGTACTCTT GGAGGGAGAT 
CCTGTCACTC TCAGCTGCCT CCCTGCTGGC AGTCCTCACC CACACATCTC ATGGATGAAA 
GACAAGAAGC CACTTGAGAT TGACCCCAGG ATGAATATGA TATCTTGCCC CGATGGGAGA 
CAACTGCTGA TGATTATGAA GACCACCAAG AAGGATGCAG GCCTCTATGA ATGTGTGGCT 
GCAAACCCAC TGGCCACAGT CACCAGCTCA TGCGTTGTGT CCCTCGCTCG TCTGCCTAAT 
AGTCCTGGGA CACCCGAGGT TCCTCAGAAG TATAAAAACA CTGCTCTAGT GGTATGGAGA 
CCCTCAGACA CTACGGCACC CTGCACCTAC TCTCTGGAGA GGAAGACAGA GGGTGAGAAT 
AACTGGCTGA TAGTCGCCAC AGGTGTTGCT GACTGTTACT ATAACGTGTT GGATCTTCCG 
GCCGGCGCTT CATACAGGTT CAGAGTGGCC TGCGTCAATA AGGCTGGTCA GGGACCTTAC 
AGCAGCTTGT CTGAAGTAGT GGTCCTGGAC TCAACAGAGC CCCAGAAGTC CAGTGGCACA 
GTTGTAGTCA AAACGCTTTC ATCAGCTCCC GCTCCTGCAG TGGTAACGTC CACCATGACC 
CTTCCTGCAA TGAAGCCTGC TGCAATAAAA CCAGCACCAT CAACAAATTC AGCGCCCTCT 
ACAGTCCAAC CGGTAAAGCC TGCGCCTCCT CCTGCATCAT CAACACCTAC CTATCGTCCC 
GTGCCTGACA TTCGAACTCC ATCGCCTGGC ACTCCAACCC CTGCCAAAGC CAAAAGCACC 
CTCAACATTA CTATGGCCAA ACCCTCCCCA GCATCCCCTG CAGCTCAACC ACAACCACAA 
CCTCCACCCA AACCCTCGCC TCCGGTCATT CTGCCCAAAC CTCAGACCCC AGTCAGTGTG 
GTTTCACCTC CATCCCAAAC TCCTCCAGTG TCACCTCCAC CTCTAGTGTC TCCTCCTCCC 
ATTATTGGTA AACCAATCTC CTCAGTGCCC ATGTATGTCC CTGCTACAAC CACCTCGACT 
CCAGTGACTC CGGTCACTCC TGTCACCCCA GTCACTCCAG TCACCCCAAA TACGCCCTCT 
CCTGCCCTGT CGCCTCCAGT GGTGTTGGTT ACAAGTCTGA GTCCTGTAGG GGAGGGAACT 
GGGACACCCA ATCGAATGAC CCCTAGTGGA AGAGCCACGC CTTCTGGACG CATCACTCCA 
TCAGGCCGAA GGACCCCTAT TGGAAAGCCT GGAGACTCAA CGCTGAGGCA AGGAGTCCCT 
CAGAAACCTT ACACCTTCAT GGATGAGAAA GCAAGGGGTC GCTTTGGTGT GATCAGAGAG 
TGTCGAGAAA ATGCCACTGG TAACCTGTAC ATGGCTAAGA TTGTGCCATA TGAGCCAGAG 
AGCAAGCAGA CTGTATTACA GGAATACGAC ATCCTGAAGT CTCTCCATCA TGAGAAGATC 
ATGGCTCTCC ACGAGGCCTA CGTCACTCCA CGCTACCTGG TGCTCATCTC TGAGTGCTGC 
TCTGGCAAAG AACTACTCCA CAGTCTCATT GACAGGTTCC GATACTCTGA GGATGATGTG 
GTTGCATATA TCGTACAGAT CCTTCAGGGT CTGGACTACC TGCACAGCCG GAGGATCCTG 
CATCTCGACA TCAAACCAGA AAACATCATC GTCACTTACA TGAATGTGGT GAAGATTATC 
GATTTTGGTA GTGCACAGAC CTTCAATCCT CTGTTCCTGA AGCAGTTCTC CCCACCTATT 
GGAACACTGG ATTATATGTC TCCAGAGATG CTGAAAGGAG ATGTGGTGGG TCCACCAGCT 
GACATCTGGA GCATTGGCAT ATTGACTTAT ATCATGCTTA GCGGGAGGTT GCCGTTCACA 
GAAAATGACC CTGCCGAGAC CGAGGCCAGA ATACAGGCAG CCAAGTTTGA CCTCAGTAAG 
CTTTACCAAA ACGTGTCCCA AAGTGCCTCT CTGTTCATCA AAAAGATCCT GTGCAGCTAC 
CCTTGGGCCC GTCCGACCAT CAAGGACTGC TTTACCAACT CGTGGCTGCA GGACGCATAT 
CTGATGCGCC TGCGAAGACA GACTCTCACA TTCACCACCA CACGCCTCAA AGAGTTCCTG 
GAGCAGCAGC AGCACATCAG AGCCCAAGAG GCAACAAAGC ACAAAGTCCT CCTGCGTTCC 
TACCAGAGCA ACCCACAAAC CCCATCCACC CCTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303170.1
CDS	3038..12916
Translation	MSQSSAQDSRPQTPVSEYSRKELTLRPSPKLTRASSKIFEKVRVFEERRRSIDNPEGSISGRSWAGFNRASSIDSDEGGSRLGISRESSKEDLREALKADAAQRRTMFKQRAASLEDRPRYTQKVQDIENKFTEELQRIKKLVGKPHMKKSFSTEQLSTCHRSRQPVRKLEPIPPQVLQKLQDRERAQLAQLEQEMRERDQAKERSPPKSPSRRRKDHLAQQPPERAETKVVSTTGLEPSPPEAMSLSDLPGQRSPRFRGPSPSRDSTRRSPTVEISAMAEERPRGRAESPSRNVLEMTLRKVEPRPASPLVKRVVRVQEVPQANDQPMHRKTPIEVSLRKLERRPESPLVQGETVAIQDFPVQAPPKPPRVSSSSSSEEKMELEVTPPPPPLKLTIPKIIVEEEPMETDTPVEKTEKAVKVGTGKDEKHQRSRGRGRRQRPMSPELESSDDSYVSAGEDPLEAPVFEFPLQDTVASTGTEVLLKCIISGTPLPEVIWKKDNTEVKNSPTHVVKIEGERHSLLIKWTKPSDAGTYTVTAVNEVGEVSSSATLFIKPEPSQDHRGNLGVPMDVSSPITSDEEYLSPLEESMDFSSYRGPEPRKIVDTRFKEPPAFLAVIGDQSVIEGQEVTMSVLVTGQPKPMLYWLRDRVTIKTGTRHIVQETEEGNFEMIIKSAQRSDTGVYTCKIINEYGTKQCEGKLEVKARPVEPGLAIIRPVRDVMVKAGETALFECHVIGPQDTDVDWLSDGKLIQPALLNCKMHFDGKRCRLLLNSVHEDDSGTYTCKLSTAKEELTSSAKLKVIASIEPLFTRKLDVLEVIEGRNARFDCKVSGTPPPQVIWSHFDRPLEENEDIRILKEGGRHSLIISHVSNEDEGLYTVAARNSHGEDECAAELYVQEPRPAISSQIAKLEKMPSIPEEPEVPENEIERFTMPDFVKPLYDMDVVEGREAVLRCKVAGLPYPTITWFHNGKRIDSTEDRKMTQFRDVHSLVVRCVCHAHGGVYKCVISNKVGKATCYSHLYVTDILPDPPDGAPVVESITGKTITLSWKKPKRLDPSIDPSSLMYAIQQQALGSIQWTIIASCLKQTTYTISNLSKGVRYAFRVLSITSKAFSKPSPATEPMQLLDRGPYVQEAPVIIDKPDIVYMVEKQPLSITVTLNHVNASVTWKRGGVTLSNRPGLFELSMPDDDQHTLKLCKVKSSDVGQLTCIANNKYGSDSCVLTLEMAVAPTFETIMEDLDVNVGETPRFAVVVEGKPVPDILWYKGDTLLSESSHFTFVYDDNECSLVVLNTQADDSGVYTCTAKNLAGSVSCKAELTVHTTKQYKDDPMEDEASILRKMRRLTDYYDVHKEIGRGAFSYVKRVIQKAGKLEYAAKFISARAKRKASALRELNILSHLDHERILYFHDAFEKKNAVIIITELCHEELLDRLTKKSTILESEIRSSVRQLLEGINYLHQLDILHLDIKPDNILMADHSSDQIRICDFGNAVKFMPDEAQYCKYGTPEFVAPEIVNQTPVSKATDIWPIGVLTYLCLTGVSPFAGENDRSSVLNIRNYNVAFEESMFTDLCHEAKGFVIKLLVADRLRPDANECLRHPWFKTLNKGKSISTESLKKFLSRRKWQRSLISYKSKMVMRSVPELLDDSSSHISIAVPRHLKKGSPPLSSSSDSDEDIDELPFIPMPLNVEFSGSRMSLTEIVGDDDMTGKLNGNAERPASLHPKSEPMEVEMSEREGGDPSARGRAKKRTTTEDEKGSSDEEPSQLAKRAELAKRPMRRGSSMESDEPEGGRRRGELRRGSSADSALQLHIKPEEGTEESPTDGSRILQKSVSMELPRRSPSPGAAKLSQEDYALKLELMRQRLLRGGSADNKMSGLRGPLLETLGMGDEKRFVRGSRLGNPPLVRAASSESPRDDIPKTKILRKSASFSQGDSEPMPLHRRIGAPLEIPLAQIEERRLQEAISMSALTEPVKDSRPITPKLQMPEEERHEKKKTEEKESVYKDNKPEEVIAVSKTEEEGRRVDQKSWDSDAQKKDQSKSEETREESEKVAEIEKKTSIVDETIEEEETRKDEEPEKETEPPTPKIEVKEYEGDVKVLPKSPEFIISTNSIMVTPTAAPASSPQSVSTYVTPSPPARTVLPDGRMSAYASIMQTIMVPSVPANTQSDTVPIPTPTSTPPSVPVYAPISRHISEPTLSSTSSSKSDLHQTTEHPAVFSRVASTEHPPKEPSPPKTPTRASPKREPSPGSNIQDLASEEVFEARFKKRESSLTRSLKRFSFQKTEDKPSTVSPETAEEMYRPGPTGAPLEFVPRRLEEKSKSVQDLREAEKDPGFMRRLSMRLKRTPSVEKQEEKLKEEEASAPRRRLSWALGRRGSQEKKEIEMMRLDGGPEPPPEPESKAPSESPILAMRKKIGSTMASLSTRIRSHSEDRKDDSEGKMETKRTPIFNKLRRSTSEGGSLKRMGIPQNQLAAQSGQGTSSESLDSMSSIQSESAIKNAELDRRSRWDRWGLTRGKKDKTVSQPDIPASIARENGSLRSRQYTRLTSDFPPVFHIKLRDHVLLEGDPVTLSCLPAGSPHPHISWMKDKKPLEIDPRMNMISCPDGRQLLMIMKTTKKDAGLYECVAANPLATVTSSCVVSLARLPNSPGTPEVPQKYKNTALVVWRPSDTTAPCTYSLERKTEGENNWLIVATGVADCYYNVLDLPAGASYRFRVACVNKAGQGPYSSLSEVVVLDSTEPQKSSGTVVVKTLSSAPAPAVVTSTMTLPAMKPAAIKPAPSTNSAPSTVQPVKPAPPPASSTPTYRPVPDIRTPSPGTPTPAKAKSTLNITMAKPSPASPAAQPQPQPPPKPSPPVILPKPQTPVSVVSPPSQTPPVSPPPLVSPPPIIGKPISSVPMYVPATTTSTPVTPVTPVTPVTPVTPNTPSPALSPPVVLVTSLSPVGEGTGTPNRMTPSGRATPSGRITPSGRRTPIGKPGDSTLRQGVPQKPYTFMDEKARGRFGVIRECRENATGNLYMAKIVPYEPESKQTVLQEYDILKSLHHEKIMALHEAYVTPRYLVLISECCSGKELLHSLIDRFRYSEDDVVAYIVQILQGLDYLHSRRILHLDIKPENIIVTYMNVVKIIDFGSAQTFNPLFLKQFSPPIGTLDYMSPEMLKGDVVGPPADIWSIGILTYIMLSGRLPFTENDPAETEARIQAAKFDLSKLYQNVSQSASLFIKKILCSYPWARPTIKDCFTNSWLQDAYLMRLRRQTLTFTTTRLKEFLEQQQHIRAQEATKHKVLLRSYQSNPQTPSTPQ

Target ORF information:

RefSeq Version	XM_009304895.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio SPEG complex locus b (spegb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304895.2

ORF Insert Sequence:

ATGTCTCAGA GTAGTGCTCA AGACTCTCGA CCCCAAACCC CTGTCAGTGA GTATTCCCGT 
AAGGAGCTTA CACTTCGGCC CTCACCAAAA CTGACAAGAG CTAGCTCCAA AATCTTTGAG 
AAGGTGCGAG TCTTCGAAGA GCGCAGAAGA AGCATTGATA ACCCTGAAGG ATCTATATCT 
GGACGTTCTT GGGCTGGATT TAACAGAGCT TCATCTATCG ACTCTGATGA AGGAGGGAGC 
AGGTTGGGCA TCTCCAGGGA GAGCTCCAAA GAAGACCTTC GTGAGGCTTT GAAGGCAGAT 
GCAGCCCAAC GAAGGACCAT GTTTAAACAG CGGGCTGCTT CCCTGGAGGA CAGACCTCGT 
TACACCCAGA AGGTGCAGGA CATTGAAAAC AAGTTCACAG AGGAGCTCCA GCGCATCAAA 
AAGCTTGTCG GCAAACCACA CATGAAGAAA TCCTTCTCAA CAGAGCAGCT CTCTACTTGT 
CACAGAAGCA GGCAGCCTGT AAGAAAGTTA GAGCCCATCC CACCTCAGGT CCTTCAAAAG 
CTGCAGGATA GAGAACGTGC TCAGCTAGCT CAGCTAGAGC AAGAGATGAG AGAGCGAGAC 
CAGGCCAAAG AACGATCACC TCCGAAATCA CCGTCTCGCA GAAGGAAAGA CCATCTGGCA 
CAACAACCAC CAGAAAGAGC TGAGACCAAG GTGGTAAGCA CAACTGGACT TGAACCATCC 
CCTCCAGAAG CCATGTCACT CTCTGATTTA CCAGGTCAAA GATCTCCAAG ATTTCGGGGC 
CCAAGCCCAA GCAGAGATAG TACACGAAGG TCTCCAACCG TAGAGATTAG TGCCATGGCT 
GAGGAGCGCC CTCGAGGAAG AGCTGAATCT CCTTCAAGAA ATGTTTTGGA GATGACGTTA 
CGCAAAGTGG AGCCAAGACC TGCAAGTCCA CTGGTAAAGA GGGTGGTTCG TGTTCAAGAA 
GTTCCACAAG CAAACGATCA GCCTATGCAT AGAAAGACGC CAATAGAGGT CTCGCTAAGA 
AAACTGGAAA GAAGGCCTGA AAGTCCCCTG GTGCAAGGAG AAACCGTAGC CATTCAAGAC 
TTCCCTGTTC AAGCTCCCCC CAAACCTCCA AGAGTTTCCT CATCTTCCTC GTCTGAGGAG 
AAGATGGAGC TGGAAGTGAC TCCTCCACCA CCCCCACTAA AACTCACTAT ACCAAAAATT 
ATTGTTGAAG AAGAGCCCAT GGAGACAGAC ACACCTGTTG AGAAGACTGA AAAGGCTGTG 
AAAGTTGGCA CTGGTAAAGA TGAAAAACAT CAAAGATCCA GAGGAAGAGG ACGCAGACAA 
AGACCAATGT CCCCTGAATT AGAATCTTCG GACGACTCCT ATGTGTCTGC AGGAGAGGAT 
CCACTAGAAG CTCCTGTGTT CGAGTTTCCA CTTCAGGACA CTGTGGCCTC CACTGGAACG 
GAGGTGCTAT TAAAATGCAT CATCAGTGGC ACCCCTCTCC CTGAAGTTAT ATGGAAGAAA 
GACAACACTG AGGTCAAGAA CAGTCCCACT CATGTTGTAA AGATAGAGGG GGAACGCCAC 
TCCTTGCTCA TCAAATGGAC AAAGCCAAGT GATGCAGGAA CCTACACAGT TACCGCTGTC 
AATGAGGTCG GTGAGGTGTC CAGCAGTGCT ACACTTTTCA TCAAACCAGA GCCCAGTCAG 
GACCATCGTG GTAACCTTGG TGTCCCTATG GATGTCAGCA GTCCCATCAC ATCTGATGAG 
GAGTATCTCA GTCCACTGGA AGAGAGCATG GACTTCTCCT CCTACAGGGG ACCGGAGCCA 
AGAAAGATCG TGGACACTCG ATTTAAGGAG CCTCCAGCCT TTCTGGCTGT TATTGGAGAC 
CAAAGCGTAA TAGAGGGTCA GGAGGTGACC ATGTCTGTTC TAGTGACCGG ACAACCTAAA 
CCTATGCTTT ACTGGCTGAG AGACAGAGTG ACCATTAAAA CCGGAACACG GCATATCGTC 
CAAGAGACAG AGGAGGGCAA CTTTGAAATG ATAATTAAAT CAGCACAGAG GTCAGACACC 
GGTGTTTACA CCTGCAAGAT CATCAATGAA TATGGAACTA AGCAATGTGA AGGCAAACTG 
GAGGTGAAAG CTCGTCCAGT AGAGCCAGGT CTGGCCATCA TTCGGCCAGT GAGGGACGTG 
ATGGTGAAGG CAGGGGAGAC CGCTCTGTTT GAGTGTCATG TGATCGGCCC GCAGGACACA 
GATGTTGACT GGCTGTCAGA TGGGAAACTC ATCCAGCCTG CTTTGTTGAA CTGCAAGATG 
CATTTTGATG GGAAAAGGTG CAGGCTGCTT CTAAACTCTG TACATGAGGA TGACAGTGGA 
ACGTACACCT GCAAACTGAG CACAGCTAAA GAGGAACTGA CCTCCAGTGC CAAGCTAAAA 
GTCATAGCAT CCATAGAGCC CCTTTTCACT CGTAAGCTGG ATGTGCTGGA AGTTATCGAG 
GGTCGTAATG CACGATTTGA CTGCAAGGTC AGCGGGACGC CGCCGCCTCA AGTCATATGG 
AGCCACTTCG ATCGTCCGCT CGAGGAAAAC GAAGATATCA GGATCTTAAA AGAAGGAGGA 
CGGCACTCAT TAATCATCTC TCATGTTAGT AATGAAGATG AGGGCCTCTA CACGGTTGCA 
GCGCGAAACT CACACGGGGA AGACGAATGT GCAGCGGAGC TCTATGTGCA GGAGCCCAGA 
CCAGCAATAT CTTCTCAAAT AGCGAAGCTG GAAAAAATGC CTTCCATTCC CGAAGAACCC 
GAAGTTCCTG AAAATGAGAT TGAGCGTTTC ACCATGCCTG ACTTCGTAAA GCCGCTTTAT 
GATATGGATG TAGTGGAGGG CAGAGAGGCT GTGCTCAGGT GCAAAGTGGC CGGCTTGCCG 
TACCCCACCA TAACATGGTT TCACAATGGC AAGAGAATTG ACAGCACGGA GGACAGAAAG 
ATGACACAGT TCAGAGATGT TCACAGCCTG GTGGTCCGCT GTGTTTGTCA TGCTCACGGA 
GGGGTTTACA AGTGTGTCAT ATCCAATAAA GTGGGCAAGG CCACCTGCTA TTCTCATCTT 
TACGTGACAG ACATCCTTCC TGACCCTCCA GATGGTGCCC CAGTCGTTGA GTCCATCACT 
GGCAAGACTA TCACGCTAAG CTGGAAGAAA CCCAAAAGAC TGGATCCGTC CATTGACCCC 
AGCTCCCTGA TGTATGCCAT TCAGCAACAG GCCCTGGGGT CGATCCAGTG GACCATCATC 
GCTTCTTGCC TGAAACAGAC CACCTATACC ATCAGCAATC TGTCTAAAGG TGTCCGCTAC 
GCCTTCAGGG TGCTATCAAT CACCAGCAAG GCCTTTAGCA AGCCGTCGCC TGCCACCGAG 
CCAATGCAGC TGCTAGATAG AGGTCCGTAC GTCCAGGAGG CTCCGGTAAT CATTGACAAG 
CCAGATATCG TGTATATGGT GGAGAAGCAG CCCTTGAGCA TCACAGTCAC TCTCAACCAC 
GTCAACGCAT CGGTCACATG GAAGAGAGGA GGAGTGACAT TATCTAACCG ACCGGGTCTT 
TTTGAGTTGA GCATGCCTGA TGATGACCAG CACACACTGA AGCTGTGTAA GGTGAAGAGC 
AGTGATGTTG GCCAGCTCAC CTGCATTGCC AACAACAAGT ACGGCAGCGA CTCCTGTGTC 
CTCACCCTGG AGATGGCAGT GGCACCAACT TTTGAGACAA TTATGGAAGA CCTGGATGTG 
AATGTAGGCG AGACTCCTCG ATTTGCAGTG GTGGTTGAAG GAAAGCCCGT CCCAGACATC 
CTCTGGTACA AGGGAGACAC CCTTCTGTCC GAAAGCAGCC ATTTCACATT TGTGTATGAT 
GACAATGAAT GTTCTCTAGT GGTGCTCAAC ACTCAAGCAG ACGACTCAGG AGTGTACACT 
TGTACTGCGA AGAATCTGGC CGGTTCTGTG TCCTGCAAGG CTGAGCTGAC CGTCCACACC 
ACCAAACAGT ACAAAGACGA CCCGATGGAG GATGAGGCGT CCATCCTCAG AAAGATGCGG 
AGGCTCACTG ATTACTATGA TGTGCACAAG GAAATTGGGA GAGGGGCTTT CTCATATGTG 
AAGCGGGTGA TACAGAAGGC AGGAAAGCTG GAATACGCAG CCAAGTTTAT TTCTGCTCGG 
GCTAAACGAA AGGCATCCGC TCTGAGAGAA CTGAACATCC TCTCTCATCT GGACCATGAG 
AGGATCCTCT ATTTTCATGA TGCCTTTGAG AAGAAGAATG CTGTCATCAT CATTACAGAG 
CTATGCCATG AGGAGCTTCT CGATAGGCTT ACAAAGAAAT CAACCATCTT GGAGTCCGAG 
ATCCGATCCA GCGTGAGGCA GCTGTTGGAG GGTATTAACT ATCTCCATCA GCTTGACATA 
CTTCATCTAG ACATCAAGCC TGATAATATC CTCATGGCAG ACCACAGTAG CGATCAGATC 
CGCATATGTG ATTTTGGAAA TGCTGTAAAG TTCATGCCTG ATGAGGCACA GTACTGCAAA 
TATGGCACTC CAGAATTTGT CGCCCCAGAG ATTGTTAACC AGACACCCGT CTCCAAGGCA 
ACGGACATCT GGCCCATTGG TGTTCTGACA TACTTATGTC TTACTGGAGT TTCTCCTTTT 
GCTGGGGAAA ACGACCGCAG CTCTGTTCTC AACATCAGGA ATTATAATGT GGCTTTTGAA 
GAAAGCATGT TTACAGACCT CTGCCATGAA GCCAAGGGAT TCGTCATCAA GCTGCTGGTG 
GCTGATAGAT TGAGGCCTGA TGCTAATGAA TGTCTTCGCC ACCCCTGGTT CAAGACCCTC 
AACAAGGGTA AAAGCATCAG TACAGAGTCC CTTAAAAAGT TTTTGTCCAG AAGAAAATGG 
CAGCGATCGC TTATCAGCTA CAAATCTAAA ATGGTGATGA GGTCTGTACC TGAGCTACTG 
GATGACTCCT CCAGCCATAT TTCCATTGCT GTTCCGCGAC ATCTGAAAAA AGGCTCACCA 
CCACTTTCAT CATCTTCAGA TTCAGATGAA GACATCGATG AGTTGCCTTT TATTCCCATG 
CCACTCAACG TGGAATTCTC AGGATCTAGA ATGTCACTGA CCGAAATTGT AGGTGATGAT 
GACATGACAG GTAAATTGAA TGGAAATGCT GAGAGGCCTG CTTCACTCCA TCCGAAGTCC 
GAGCCAATGG AAGTGGAGAT GTCAGAGAGA GAAGGTGGAG ATCCATCTGC TAGGGGTAGA 
GCCAAGAAGA GGACTACAAC AGAAGATGAA AAAGGGTCGT CTGATGAGGA ACCATCACAA 
CTGGCCAAAA GAGCAGAACT GGCAAAGAGA CCAATGCGCA GAGGTTCAAG CATGGAGTCA 
GATGAGCCAG AGGGAGGACG TCGCAGAGGA GAGTTACGCA GAGGGAGTTC GGCTGACAGT 
GCCTTACAGC TCCATATCAA ACCAGAGGAG GGCACAGAAG AAAGTCCTAC TGATGGAAGT 
AGAATTCTAC AAAAGTCTGT ATCTATGGAG CTTCCAAGGA GAAGCCCAAG TCCAGGTGCT 
GCAAAGCTTA GCCAGGAAGA CTATGCTCTG AAACTGGAAC TGATGAGGCA GCGACTACTC 
AGGGGTGGCT CTGCTGACAA CAAGATGAGT GGATTACGAG GCCCATTGCT GGAGACACTA 
GGTATGGGAG ATGAGAAGCG GTTTGTGCGG GGATCTCGTC TAGGTAACCC TCCACTTGTT 
AGAGCGGCAT CTAGTGAGTC ACCTAGAGAT GACATCCCAA AGACAAAGAT TCTCCGCAAG 
AGTGCTTCCT TTAGCCAGGG GGATTCAGAG CCCATGCCCC TGCACAGAAG AATCGGAGCA 
CCTCTAGAGA TTCCTCTAGC ACAAATAGAG GAGAGACGCC TACAGGAAGC AATATCCATG 
TCTGCTCTCA CAGAGCCTGT CAAAGATTCT CGCCCAATCA CTCCAAAACT TCAAATGCCT 
GAAGAAGAAA GACATGAAAA GAAGAAAACT GAAGAAAAAG AATCTGTCTA CAAGGACAAT 
AAACCTGAAG AGGTTATTGC TGTGTCAAAG ACTGAGGAAG AAGGAAGACG TGTTGATCAG 
AAATCTTGGG ATTCTGATGC ACAGAAAAAG GACCAGAGCA AGTCAGAGGA AACCAGGGAA 
GAGTCTGAAA AAGTGGCTGA AATTGAAAAA AAGACCTCTA TAGTGGACGA AACAATTGAG 
GAAGAGGAAA CAAGAAAAGA TGAGGAGCCA GAGAAAGAAA CTGAGCCTCC TACTCCAAAG 
ATTGAGGTTA AGGAATATGA GGGTGATGTA AAGGTATTAC CAAAGTCTCC AGAGTTTATC 
ATTTCCACAA ACTCAATCAT GGTGACCCCA ACAGCTGCCC CAGCATCCAG TCCCCAGAGT 
GTATCAACCT ATGTTACCCC ATCTCCCCCT GCACGCACAG TGCTCCCAGA TGGTAGGATG 
TCCGCATATG CCAGTATTAT GCAGACTATC ATGGTACCAT CTGTCCCAGC AAATACCCAA 
TCTGACACTG TTCCTATTCC TACACCAACT TCCACTCCAC CATCAGTTCC AGTCTATGCA 
CCCATTTCCA GACACATCTC AGAACCAACC CTTTCATCAA CTTCTTCATC CAAATCAGAT 
CTTCACCAAA CAACTGAGCA TCCTGCAGTC TTTTCTCGGG TTGCCTCCAC TGAACACCCT 
CCCAAAGAGC CAAGTCCTCC TAAGACACCC ACTCGCGCCT CCCCTAAGCG AGAGCCATCT 
CCTGGAAGCA ACATCCAGGA CCTTGCATCC GAGGAGGTTT TTGAAGCTCG CTTCAAGAAA 
CGTGAGTCTT CTCTAACTCG TAGTCTCAAA CGTTTCTCAT TTCAGAAGAC TGAAGACAAG 
CCCTCCACTG TTTCTCCTGA AACTGCTGAA GAAATGTATC GCCCTGGACC AACTGGTGCT 
CCTTTAGAGT TTGTGCCAAG GAGGTTGGAG GAGAAGTCCA AATCAGTACA AGATCTTCGA 
GAGGCAGAGA AGGACCCTGG GTTCATGAGG CGACTCTCCA TGCGCTTGAA AAGGACCCCA 
TCAGTGGAAA AGCAGGAGGA GAAGCTCAAA GAGGAGGAGG CTTCAGCACC CAGGCGTCGT 
CTTTCTTGGG CACTGGGACG GAGGGGATCC CAAGAGAAGA AGGAGATTGA GATGATGAGG 
TTGGATGGGG GTCCTGAGCC TCCCCCAGAG CCTGAGTCTA AAGCACCTTC TGAGTCACCT 
ATTCTTGCCA TGCGCAAGAA GATTGGCAGC ACCATGGCAA GCTTATCTAC AAGAATTAGG 
AGCCATTCTG AGGACAGGAA AGATGACAGT GAAGGCAAGA TGGAGACCAA GAGGACTCCT 
ATATTCAACA AACTAAGGCG TTCCACTTCT GAGGGGGGCA GTCTGAAGAG AATGGGTATC 
CCACAAAACC AACTGGCTGC TCAGTCAGGG CAAGGGACCT CATCAGAATC TCTTGATTCT 
ATGTCAAGCA TCCAGTCAGA ATCAGCTATT AAAAATGCAG AACTTGACCG CAGATCACGG 
TGGGACCGAT GGGGCTTGAC TCGAGGGAAA AAGGACAAGA CAGTTTCCCA ACCAGACATC 
CCAGCTTCCA TTGCTAGAGA GAATGGTTCT CTACGGTCTC GCCAATATAC ACGTCTAACA 
TCTGACTTCC CTCCGGTGTT CCATATCAAA CTGAGGGACC ATGTACTCTT GGAGGGAGAT 
CCTGTCACTC TCAGCTGCCT CCCTGCTGGC AGTCCTCACC CACACATCTC ATGGATGAAA 
GACAAGAAGC CACTTGAGAT TGACCCCAGG ATGAATATGA TATCTTGCCC CGATGGGAGA 
CAACTGCTGA TGATTATGAA GACCACCAAG AAGGATGCAG GCCTCTATGA ATGTGTGGCT 
GCAAACCCAC TGGCCACAGT CACCAGCTCA TGCGTTGTGT CCCTCGCTCG TCTGCCTAAT 
AGTCCTGGGA CACCCGAGGT TCCTCAGAAG TATAAAAACA CTGCTCTAGT GGTATGGAGA 
CCCTCAGACA CTACGGCACC CTGCACCTAC TCTCTGGAGA GGAAGACAGA GGGTGAGAAT 
AACTGGCTGA TAGTCGCCAC AGGTGTTGCT GACTGTTACT ATAACGTGTT GGATCTTCCG 
GCCGGCGCTT CATACAGGTT CAGAGTGGCC TGCGTCAATA AGGCTGGTCA GGGACCTTAC 
AGCAGCTTGT CTGAAGTAGT GGTCCTGGAC TCAACAGAGC CCCAGAAGTC CAGTGGCACA 
GTTGTAGTCA AAACGCTTTC ATCAGCTCCC GCTCCTGCAG TGGTAACGTC CACCATGACC 
CTTCCTGCAA TGAAGCCTGC TGCAATAAAA CCAGCACCAT CAACAAATTC AGCGCCCTCT 
ACAGTCCAAC CGGTAAAGCC TGCGCCTCCT CCTGCATCAT CAACACCTAC CTATCGTCCC 
GTGCCTGACA TTCGAACTCC ATCGCCTGGC ACTCCAACCC CTGCCAAAGC CAAAAGCACC 
CTCAACATTA CTATGGCCAA ACCCTCCCCA GCATCCCCTG CAGCTCAACC ACAACCACAA 
CCTCCACCCA AACCCTCGCC TCCGGTCATT CTGCCCAAAC CTCAGACCCC AGTCAGTGTG 
GTTTCACCTC CATCCCAAAC TCCTCCAGTG TCACCTCCAC CTCTAGTGTC TCCTCCTCCC 
ATTATTGGTA AACCAATCTC CTCAGTGCCC ATGTATGTCC CTGCTACAAC CACCTCGACT 
CCAGTGACTC CGGTCACTCC TGTCACCCCA GTCACTCCAG TCACCCCAAA TACGCCCTCT 
CCTGCCCTGT CGCCTCCAGT GGTGTTGGTT ACAAGTCTGA GTCCTGTAGG GGAGGGAACT 
GGGACACCCA ATCGAATGAC CCCTAGTGGA AGAGCCACGC CTTCTGGACG CATCACTCCA 
TCAGGCCGAA GGACCCCTAT TGGAAAGCCT GGAGACTCAA CGCTGAGGCA AGGAGTCCCT 
CAGAAACCTT ACACCTTCAT GGATGAGAAA GCAAGGGGTC GCTTTGGTGT GATCAGAGAG 
TGTCGAGAAA ATGCCACTGG TAACCTGTAC ATGGCTAAGA TTGTGCCATA TGAGCCAGAG 
AGCAAGCAGA CTGTATTACA GGAATACGAC ATCCTGAAGT CTCTCCATCA TGAGAAGATC 
ATGGCTCTCC ACGAGGCCTA CGTCACTCCA CGCTACCTGG TGCTCATCTC TGAGTGCTGC 
TCTGGCAAAG AACTACTCCA CAGTCTCATT GACAGGTTCC GATACTCTGA GGATGATGTG 
GTTGCATATA TCGTACAGAT CCTTCAGGGT CTGGACTACC TGCACAGCCG GAGGATCCTG 
CATCTCGACA TCAAACCAGA AAACATCATC GTCACTTACA TGAATGTGGT GAAGATTATC 
GATTTTGGTA GTGCACAGAC CTTCAATCCT CTGTTCCTGA AGCAGTTCTC CCCACCTATT 
GGAACACTGG ATTATATGTC TCCAGAGATG CTGAAAGGAG ATGTGGTGGG TCCACCAGCT 
GACATCTGGA GCATTGGCAT ATTGACTTAT ATCATGCTTA GCGGGAGGTT GCCGTTCACA 
GAAAATGACC CTGCCGAGAC CGAGGCCAGA ATACAGGCAG CCAAGTTTGA CCTCAGTAAG 
CTTTACCAAA ACGTGTCCCA AAGTGCCTCT CTGTTCATCA AAAAGATCCT GTGCAGCTAC 
CCTTGGGCCC GTCCGACCAT CAAGGACTGC TTTACCAACT CGTGGCTGCA GGACGCATAT 
CTGATGCGCC TGCGAAGACA GACTCTCACA TTCACCACCA CACGCCTCAA AGAGTTCCTG 
GAGCAGCAGC AGCACATCAG AGCCCAAGAG GCAACAAAGC ACAAAGTCCT CCTGCGTTCC 
TACCAGAGCA ACCCACAAAC CCCATCCACC CCTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spegb ( XM_009304896.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009303171.1 Danio rerio SPEG complex locus b (spegb), transcript variant X2, mRNA.

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CloneID	ODa43992
Clone ID Related Accession (Same CDS sequence)	XM_009304895.2 , XM_009304896.2
Accession Version	XM_009304896.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9879bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	striated muscle preferentially expressed protein kinase
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304896.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGA GTAGTGCTCA AGACTCTCGA CCCCAAACCC CTGTCAGTGA GTATTCCCGT 
AAGGAGCTTA CACTTCGGCC CTCACCAAAA CTGACAAGAG CTAGCTCCAA AATCTTTGAG 
AAGGTGCGAG TCTTCGAAGA GCGCAGAAGA AGCATTGATA ACCCTGAAGG ATCTATATCT 
GGACGTTCTT GGGCTGGATT TAACAGAGCT TCATCTATCG ACTCTGATGA AGGAGGGAGC 
AGGTTGGGCA TCTCCAGGGA GAGCTCCAAA GAAGACCTTC GTGAGGCTTT GAAGGCAGAT 
GCAGCCCAAC GAAGGACCAT GTTTAAACAG CGGGCTGCTT CCCTGGAGGA CAGACCTCGT 
TACACCCAGA AGGTGCAGGA CATTGAAAAC AAGTTCACAG AGGAGCTCCA GCGCATCAAA 
AAGCTTGTCG GCAAACCACA CATGAAGAAA TCCTTCTCAA CAGAGCAGCT CTCTACTTGT 
CACAGAAGCA GGCAGCCTGT AAGAAAGTTA GAGCCCATCC CACCTCAGGT CCTTCAAAAG 
CTGCAGGATA GAGAACGTGC TCAGCTAGCT CAGCTAGAGC AAGAGATGAG AGAGCGAGAC 
CAGGCCAAAG AACGATCACC TCCGAAATCA CCGTCTCGCA GAAGGAAAGA CCATCTGGCA 
CAACAACCAC CAGAAAGAGC TGAGACCAAG GTGGTAAGCA CAACTGGACT TGAACCATCC 
CCTCCAGAAG CCATGTCACT CTCTGATTTA CCAGGTCAAA GATCTCCAAG ATTTCGGGGC 
CCAAGCCCAA GCAGAGATAG TACACGAAGG TCTCCAACCG TAGAGATTAG TGCCATGGCT 
GAGGAGCGCC CTCGAGGAAG AGCTGAATCT CCTTCAAGAA ATGTTTTGGA GATGACGTTA 
CGCAAAGTGG AGCCAAGACC TGCAAGTCCA CTGGTAAAGA GGGTGGTTCG TGTTCAAGAA 
GTTCCACAAG CAAACGATCA GCCTATGCAT AGAAAGACGC CAATAGAGGT CTCGCTAAGA 
AAACTGGAAA GAAGGCCTGA AAGTCCCCTG GTGCAAGGAG AAACCGTAGC CATTCAAGAC 
TTCCCTGTTC AAGCTCCCCC CAAACCTCCA AGAGTTTCCT CATCTTCCTC GTCTGAGGAG 
AAGATGGAGC TGGAAGTGAC TCCTCCACCA CCCCCACTAA AACTCACTAT ACCAAAAATT 
ATTGTTGAAG AAGAGCCCAT GGAGACAGAC ACACCTGTTG AGAAGACTGA AAAGGCTGTG 
AAAGTTGGCA CTGGTAAAGA TGAAAAACAT CAAAGATCCA GAGGAAGAGG ACGCAGACAA 
AGACCAATGT CCCCTGAATT AGAATCTTCG GACGACTCCT ATGTGTCTGC AGGAGAGGAT 
CCACTAGAAG CTCCTGTGTT CGAGTTTCCA CTTCAGGACA CTGTGGCCTC CACTGGAACG 
GAGGTGCTAT TAAAATGCAT CATCAGTGGC ACCCCTCTCC CTGAAGTTAT ATGGAAGAAA 
GACAACACTG AGGTCAAGAA CAGTCCCACT CATGTTGTAA AGATAGAGGG GGAACGCCAC 
TCCTTGCTCA TCAAATGGAC AAAGCCAAGT GATGCAGGAA CCTACACAGT TACCGCTGTC 
AATGAGGTCG GTGAGGTGTC CAGCAGTGCT ACACTTTTCA TCAAACCAGA GCCCAGTCAG 
GACCATCGTG GTAACCTTGG TGTCCCTATG GATGTCAGCA GTCCCATCAC ATCTGATGAG 
GAGTATCTCA GTCCACTGGA AGAGAGCATG GACTTCTCCT CCTACAGGGG ACCGGAGCCA 
AGAAAGATCG TGGACACTCG ATTTAAGGAG CCTCCAGCCT TTCTGGCTGT TATTGGAGAC 
CAAAGCGTAA TAGAGGGTCA GGAGGTGACC ATGTCTGTTC TAGTGACCGG ACAACCTAAA 
CCTATGCTTT ACTGGCTGAG AGACAGAGTG ACCATTAAAA CCGGAACACG GCATATCGTC 
CAAGAGACAG AGGAGGGCAA CTTTGAAATG ATAATTAAAT CAGCACAGAG GTCAGACACC 
GGTGTTTACA CCTGCAAGAT CATCAATGAA TATGGAACTA AGCAATGTGA AGGCAAACTG 
GAGGTGAAAG CTCGTCCAGT AGAGCCAGGT CTGGCCATCA TTCGGCCAGT GAGGGACGTG 
ATGGTGAAGG CAGGGGAGAC CGCTCTGTTT GAGTGTCATG TGATCGGCCC GCAGGACACA 
GATGTTGACT GGCTGTCAGA TGGGAAACTC ATCCAGCCTG CTTTGTTGAA CTGCAAGATG 
CATTTTGATG GGAAAAGGTG CAGGCTGCTT CTAAACTCTG TACATGAGGA TGACAGTGGA 
ACGTACACCT GCAAACTGAG CACAGCTAAA GAGGAACTGA CCTCCAGTGC CAAGCTAAAA 
GTCATAGCAT CCATAGAGCC CCTTTTCACT CGTAAGCTGG ATGTGCTGGA AGTTATCGAG 
GGTCGTAATG CACGATTTGA CTGCAAGGTC AGCGGGACGC CGCCGCCTCA AGTCATATGG 
AGCCACTTCG ATCGTCCGCT CGAGGAAAAC GAAGATATCA GGATCTTAAA AGAAGGAGGA 
CGGCACTCAT TAATCATCTC TCATGTTAGT AATGAAGATG AGGGCCTCTA CACGGTTGCA 
GCGCGAAACT CACACGGGGA AGACGAATGT GCAGCGGAGC TCTATGTGCA GGAGCCCAGA 
CCAGCAATAT CTTCTCAAAT AGCGAAGCTG GAAAAAATGC CTTCCATTCC CGAAGAACCC 
GAAGTTCCTG AAAATGAGAT TGAGCGTTTC ACCATGCCTG ACTTCGTAAA GCCGCTTTAT 
GATATGGATG TAGTGGAGGG CAGAGAGGCT GTGCTCAGGT GCAAAGTGGC CGGCTTGCCG 
TACCCCACCA TAACATGGTT TCACAATGGC AAGAGAATTG ACAGCACGGA GGACAGAAAG 
ATGACACAGT TCAGAGATGT TCACAGCCTG GTGGTCCGCT GTGTTTGTCA TGCTCACGGA 
GGGGTTTACA AGTGTGTCAT ATCCAATAAA GTGGGCAAGG CCACCTGCTA TTCTCATCTT 
TACGTGACAG ACATCCTTCC TGACCCTCCA GATGGTGCCC CAGTCGTTGA GTCCATCACT 
GGCAAGACTA TCACGCTAAG CTGGAAGAAA CCCAAAAGAC TGGATCCGTC CATTGACCCC 
AGCTCCCTGA TGTATGCCAT TCAGCAACAG GCCCTGGGGT CGATCCAGTG GACCATCATC 
GCTTCTTGCC TGAAACAGAC CACCTATACC ATCAGCAATC TGTCTAAAGG TGTCCGCTAC 
GCCTTCAGGG TGCTATCAAT CACCAGCAAG GCCTTTAGCA AGCCGTCGCC TGCCACCGAG 
CCAATGCAGC TGCTAGATAG AGGTCCGTAC GTCCAGGAGG CTCCGGTAAT CATTGACAAG 
CCAGATATCG TGTATATGGT GGAGAAGCAG CCCTTGAGCA TCACAGTCAC TCTCAACCAC 
GTCAACGCAT CGGTCACATG GAAGAGAGGA GGAGTGACAT TATCTAACCG ACCGGGTCTT 
TTTGAGTTGA GCATGCCTGA TGATGACCAG CACACACTGA AGCTGTGTAA GGTGAAGAGC 
AGTGATGTTG GCCAGCTCAC CTGCATTGCC AACAACAAGT ACGGCAGCGA CTCCTGTGTC 
CTCACCCTGG AGATGGCAGT GGCACCAACT TTTGAGACAA TTATGGAAGA CCTGGATGTG 
AATGTAGGCG AGACTCCTCG ATTTGCAGTG GTGGTTGAAG GAAAGCCCGT CCCAGACATC 
CTCTGGTACA AGGGAGACAC CCTTCTGTCC GAAAGCAGCC ATTTCACATT TGTGTATGAT 
GACAATGAAT GTTCTCTAGT GGTGCTCAAC ACTCAAGCAG ACGACTCAGG AGTGTACACT 
TGTACTGCGA AGAATCTGGC CGGTTCTGTG TCCTGCAAGG CTGAGCTGAC CGTCCACACC 
ACCAAACAGT ACAAAGACGA CCCGATGGAG GATGAGGCGT CCATCCTCAG AAAGATGCGG 
AGGCTCACTG ATTACTATGA TGTGCACAAG GAAATTGGGA GAGGGGCTTT CTCATATGTG 
AAGCGGGTGA TACAGAAGGC AGGAAAGCTG GAATACGCAG CCAAGTTTAT TTCTGCTCGG 
GCTAAACGAA AGGCATCCGC TCTGAGAGAA CTGAACATCC TCTCTCATCT GGACCATGAG 
AGGATCCTCT ATTTTCATGA TGCCTTTGAG AAGAAGAATG CTGTCATCAT CATTACAGAG 
CTATGCCATG AGGAGCTTCT CGATAGGCTT ACAAAGAAAT CAACCATCTT GGAGTCCGAG 
ATCCGATCCA GCGTGAGGCA GCTGTTGGAG GGTATTAACT ATCTCCATCA GCTTGACATA 
CTTCATCTAG ACATCAAGCC TGATAATATC CTCATGGCAG ACCACAGTAG CGATCAGATC 
CGCATATGTG ATTTTGGAAA TGCTGTAAAG TTCATGCCTG ATGAGGCACA GTACTGCAAA 
TATGGCACTC CAGAATTTGT CGCCCCAGAG ATTGTTAACC AGACACCCGT CTCCAAGGCA 
ACGGACATCT GGCCCATTGG TGTTCTGACA TACTTATGTC TTACTGGAGT TTCTCCTTTT 
GCTGGGGAAA ACGACCGCAG CTCTGTTCTC AACATCAGGA ATTATAATGT GGCTTTTGAA 
GAAAGCATGT TTACAGACCT CTGCCATGAA GCCAAGGGAT TCGTCATCAA GCTGCTGGTG 
GCTGATAGAT TGAGGCCTGA TGCTAATGAA TGTCTTCGCC ACCCCTGGTT CAAGACCCTC 
AACAAGGGTA AAAGCATCAG TACAGAGTCC CTTAAAAAGT TTTTGTCCAG AAGAAAATGG 
CAGCGATCGC TTATCAGCTA CAAATCTAAA ATGGTGATGA GGTCTGTACC TGAGCTACTG 
GATGACTCCT CCAGCCATAT TTCCATTGCT GTTCCGCGAC ATCTGAAAAA AGGCTCACCA 
CCACTTTCAT CATCTTCAGA TTCAGATGAA GACATCGATG AGTTGCCTTT TATTCCCATG 
CCACTCAACG TGGAATTCTC AGGATCTAGA ATGTCACTGA CCGAAATTGT AGGTGATGAT 
GACATGACAG GTAAATTGAA TGGAAATGCT GAGAGGCCTG CTTCACTCCA TCCGAAGTCC 
GAGCCAATGG AAGTGGAGAT GTCAGAGAGA GAAGGTGGAG ATCCATCTGC TAGGGGTAGA 
GCCAAGAAGA GGACTACAAC AGAAGATGAA AAAGGGTCGT CTGATGAGGA ACCATCACAA 
CTGGCCAAAA GAGCAGAACT GGCAAAGAGA CCAATGCGCA GAGGTTCAAG CATGGAGTCA 
GATGAGCCAG AGGGAGGACG TCGCAGAGGA GAGTTACGCA GAGGGAGTTC GGCTGACAGT 
GCCTTACAGC TCCATATCAA ACCAGAGGAG GGCACAGAAG AAAGTCCTAC TGATGGAAGT 
AGAATTCTAC AAAAGTCTGT ATCTATGGAG CTTCCAAGGA GAAGCCCAAG TCCAGGTGCT 
GCAAAGCTTA GCCAGGAAGA CTATGCTCTG AAACTGGAAC TGATGAGGCA GCGACTACTC 
AGGGGTGGCT CTGCTGACAA CAAGATGAGT GGATTACGAG GCCCATTGCT GGAGACACTA 
GGTATGGGAG ATGAGAAGCG GTTTGTGCGG GGATCTCGTC TAGGTAACCC TCCACTTGTT 
AGAGCGGCAT CTAGTGAGTC ACCTAGAGAT GACATCCCAA AGACAAAGAT TCTCCGCAAG 
AGTGCTTCCT TTAGCCAGGG GGATTCAGAG CCCATGCCCC TGCACAGAAG AATCGGAGCA 
CCTCTAGAGA TTCCTCTAGC ACAAATAGAG GAGAGACGCC TACAGGAAGC AATATCCATG 
TCTGCTCTCA CAGAGCCTGT CAAAGATTCT CGCCCAATCA CTCCAAAACT TCAAATGCCT 
GAAGAAGAAA GACATGAAAA GAAGAAAACT GAAGAAAAAG AATCTGTCTA CAAGGACAAT 
AAACCTGAAG AGGTTATTGC TGTGTCAAAG ACTGAGGAAG AAGGAAGACG TGTTGATCAG 
AAATCTTGGG ATTCTGATGC ACAGAAAAAG GACCAGAGCA AGTCAGAGGA AACCAGGGAA 
GAGTCTGAAA AAGTGGCTGA AATTGAAAAA AAGACCTCTA TAGTGGACGA AACAATTGAG 
GAAGAGGAAA CAAGAAAAGA TGAGGAGCCA GAGAAAGAAA CTGAGCCTCC TACTCCAAAG 
ATTGAGGTTA AGGAATATGA GGGTGATGTA AAGGTATTAC CAAAGTCTCC AGAGTTTATC 
ATTTCCACAA ACTCAATCAT GGTGACCCCA ACAGCTGCCC CAGCATCCAG TCCCCAGAGT 
GTATCAACCT ATGTTACCCC ATCTCCCCCT GCACGCACAG TGCTCCCAGA TGGTAGGATG 
TCCGCATATG CCAGTATTAT GCAGACTATC ATGGTACCAT CTGTCCCAGC AAATACCCAA 
TCTGACACTG TTCCTATTCC TACACCAACT TCCACTCCAC CATCAGTTCC AGTCTATGCA 
CCCATTTCCA GACACATCTC AGAACCAACC CTTTCATCAA CTTCTTCATC CAAATCAGAT 
CTTCACCAAA CAACTGAGCA TCCTGCAGTC TTTTCTCGGG TTGCCTCCAC TGAACACCCT 
CCCAAAGAGC CAAGTCCTCC TAAGACACCC ACTCGCGCCT CCCCTAAGCG AGAGCCATCT 
CCTGGAAGCA ACATCCAGGA CCTTGCATCC GAGGAGGTTT TTGAAGCTCG CTTCAAGAAA 
CGTGAGTCTT CTCTAACTCG TAGTCTCAAA CGTTTCTCAT TTCAGAAGAC TGAAGACAAG 
CCCTCCACTG TTTCTCCTGA AACTGCTGAA GAAATGTATC GCCCTGGACC AACTGGTGCT 
CCTTTAGAGT TTGTGCCAAG GAGGTTGGAG GAGAAGTCCA AATCAGTACA AGATCTTCGA 
GAGGCAGAGA AGGACCCTGG GTTCATGAGG CGACTCTCCA TGCGCTTGAA AAGGACCCCA 
TCAGTGGAAA AGCAGGAGGA GAAGCTCAAA GAGGAGGAGG CTTCAGCACC CAGGCGTCGT 
CTTTCTTGGG CACTGGGACG GAGGGGATCC CAAGAGAAGA AGGAGATTGA GATGATGAGG 
TTGGATGGGG GTCCTGAGCC TCCCCCAGAG CCTGAGTCTA AAGCACCTTC TGAGTCACCT 
ATTCTTGCCA TGCGCAAGAA GATTGGCAGC ACCATGGCAA GCTTATCTAC AAGAATTAGG 
AGCCATTCTG AGGACAGGAA AGATGACAGT GAAGGCAAGA TGGAGACCAA GAGGACTCCT 
ATATTCAACA AACTAAGGCG TTCCACTTCT GAGGGGGGCA GTCTGAAGAG AATGGGTATC 
CCACAAAACC AACTGGCTGC TCAGTCAGGG CAAGGGACCT CATCAGAATC TCTTGATTCT 
ATGTCAAGCA TCCAGTCAGA ATCAGCTATT AAAAATGCAG AACTTGACCG CAGATCACGG 
TGGGACCGAT GGGGCTTGAC TCGAGGGAAA AAGGACAAGA CAGTTTCCCA ACCAGACATC 
CCAGCTTCCA TTGCTAGAGA GAATGGTTCT CTACGGTCTC GCCAATATAC ACGTCTAACA 
TCTGACTTCC CTCCGGTGTT CCATATCAAA CTGAGGGACC ATGTACTCTT GGAGGGAGAT 
CCTGTCACTC TCAGCTGCCT CCCTGCTGGC AGTCCTCACC CACACATCTC ATGGATGAAA 
GACAAGAAGC CACTTGAGAT TGACCCCAGG ATGAATATGA TATCTTGCCC CGATGGGAGA 
CAACTGCTGA TGATTATGAA GACCACCAAG AAGGATGCAG GCCTCTATGA ATGTGTGGCT 
GCAAACCCAC TGGCCACAGT CACCAGCTCA TGCGTTGTGT CCCTCGCTCG TCTGCCTAAT 
AGTCCTGGGA CACCCGAGGT TCCTCAGAAG TATAAAAACA CTGCTCTAGT GGTATGGAGA 
CCCTCAGACA CTACGGCACC CTGCACCTAC TCTCTGGAGA GGAAGACAGA GGGTGAGAAT 
AACTGGCTGA TAGTCGCCAC AGGTGTTGCT GACTGTTACT ATAACGTGTT GGATCTTCCG 
GCCGGCGCTT CATACAGGTT CAGAGTGGCC TGCGTCAATA AGGCTGGTCA GGGACCTTAC 
AGCAGCTTGT CTGAAGTAGT GGTCCTGGAC TCAACAGAGC CCCAGAAGTC CAGTGGCACA 
GTTGTAGTCA AAACGCTTTC ATCAGCTCCC GCTCCTGCAG TGGTAACGTC CACCATGACC 
CTTCCTGCAA TGAAGCCTGC TGCAATAAAA CCAGCACCAT CAACAAATTC AGCGCCCTCT 
ACAGTCCAAC CGGTAAAGCC TGCGCCTCCT CCTGCATCAT CAACACCTAC CTATCGTCCC 
GTGCCTGACA TTCGAACTCC ATCGCCTGGC ACTCCAACCC CTGCCAAAGC CAAAAGCACC 
CTCAACATTA CTATGGCCAA ACCCTCCCCA GCATCCCCTG CAGCTCAACC ACAACCACAA 
CCTCCACCCA AACCCTCGCC TCCGGTCATT CTGCCCAAAC CTCAGACCCC AGTCAGTGTG 
GTTTCACCTC CATCCCAAAC TCCTCCAGTG TCACCTCCAC CTCTAGTGTC TCCTCCTCCC 
ATTATTGGTA AACCAATCTC CTCAGTGCCC ATGTATGTCC CTGCTACAAC CACCTCGACT 
CCAGTGACTC CGGTCACTCC TGTCACCCCA GTCACTCCAG TCACCCCAAA TACGCCCTCT 
CCTGCCCTGT CGCCTCCAGT GGTGTTGGTT ACAAGTCTGA GTCCTGTAGG GGAGGGAACT 
GGGACACCCA ATCGAATGAC CCCTAGTGGA AGAGCCACGC CTTCTGGACG CATCACTCCA 
TCAGGCCGAA GGACCCCTAT TGGAAAGCCT GGAGACTCAA CGCTGAGGCA AGGAGTCCCT 
CAGAAACCTT ACACCTTCAT GGATGAGAAA GCAAGGGGTC GCTTTGGTGT GATCAGAGAG 
TGTCGAGAAA ATGCCACTGG TAACCTGTAC ATGGCTAAGA TTGTGCCATA TGAGCCAGAG 
AGCAAGCAGA CTGTATTACA GGAATACGAC ATCCTGAAGT CTCTCCATCA TGAGAAGATC 
ATGGCTCTCC ACGAGGCCTA CGTCACTCCA CGCTACCTGG TGCTCATCTC TGAGTGCTGC 
TCTGGCAAAG AACTACTCCA CAGTCTCATT GACAGGTTCC GATACTCTGA GGATGATGTG 
GTTGCATATA TCGTACAGAT CCTTCAGGGT CTGGACTACC TGCACAGCCG GAGGATCCTG 
CATCTCGACA TCAAACCAGA AAACATCATC GTCACTTACA TGAATGTGGT GAAGATTATC 
GATTTTGGTA GTGCACAGAC CTTCAATCCT CTGTTCCTGA AGCAGTTCTC CCCACCTATT 
GGAACACTGG ATTATATGTC TCCAGAGATG CTGAAAGGAG ATGTGGTGGG TCCACCAGCT 
GACATCTGGA GCATTGGCAT ATTGACTTAT ATCATGCTTA GCGGGAGGTT GCCGTTCACA 
GAAAATGACC CTGCCGAGAC CGAGGCCAGA ATACAGGCAG CCAAGTTTGA CCTCAGTAAG 
CTTTACCAAA ACGTGTCCCA AAGTGCCTCT CTGTTCATCA AAAAGATCCT GTGCAGCTAC 
CCTTGGGCCC GTCCGACCAT CAAGGACTGC TTTACCAACT CGTGGCTGCA GGACGCATAT 
CTGATGCGCC TGCGAAGACA GACTCTCACA TTCACCACCA CACGCCTCAA AGAGTTCCTG 
GAGCAGCAGC AGCACATCAG AGCCCAAGAG GCAACAAAGC ACAAAGTCCT CCTGCGTTCC 
TACCAGAGCA ACCCACAAAC CCCATCCACC CCTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303171.1
CDS	482..10360
Translation	MSQSSAQDSRPQTPVSEYSRKELTLRPSPKLTRASSKIFEKVRVFEERRRSIDNPEGSISGRSWAGFNRASSIDSDEGGSRLGISRESSKEDLREALKADAAQRRTMFKQRAASLEDRPRYTQKVQDIENKFTEELQRIKKLVGKPHMKKSFSTEQLSTCHRSRQPVRKLEPIPPQVLQKLQDRERAQLAQLEQEMRERDQAKERSPPKSPSRRRKDHLAQQPPERAETKVVSTTGLEPSPPEAMSLSDLPGQRSPRFRGPSPSRDSTRRSPTVEISAMAEERPRGRAESPSRNVLEMTLRKVEPRPASPLVKRVVRVQEVPQANDQPMHRKTPIEVSLRKLERRPESPLVQGETVAIQDFPVQAPPKPPRVSSSSSSEEKMELEVTPPPPPLKLTIPKIIVEEEPMETDTPVEKTEKAVKVGTGKDEKHQRSRGRGRRQRPMSPELESSDDSYVSAGEDPLEAPVFEFPLQDTVASTGTEVLLKCIISGTPLPEVIWKKDNTEVKNSPTHVVKIEGERHSLLIKWTKPSDAGTYTVTAVNEVGEVSSSATLFIKPEPSQDHRGNLGVPMDVSSPITSDEEYLSPLEESMDFSSYRGPEPRKIVDTRFKEPPAFLAVIGDQSVIEGQEVTMSVLVTGQPKPMLYWLRDRVTIKTGTRHIVQETEEGNFEMIIKSAQRSDTGVYTCKIINEYGTKQCEGKLEVKARPVEPGLAIIRPVRDVMVKAGETALFECHVIGPQDTDVDWLSDGKLIQPALLNCKMHFDGKRCRLLLNSVHEDDSGTYTCKLSTAKEELTSSAKLKVIASIEPLFTRKLDVLEVIEGRNARFDCKVSGTPPPQVIWSHFDRPLEENEDIRILKEGGRHSLIISHVSNEDEGLYTVAARNSHGEDECAAELYVQEPRPAISSQIAKLEKMPSIPEEPEVPENEIERFTMPDFVKPLYDMDVVEGREAVLRCKVAGLPYPTITWFHNGKRIDSTEDRKMTQFRDVHSLVVRCVCHAHGGVYKCVISNKVGKATCYSHLYVTDILPDPPDGAPVVESITGKTITLSWKKPKRLDPSIDPSSLMYAIQQQALGSIQWTIIASCLKQTTYTISNLSKGVRYAFRVLSITSKAFSKPSPATEPMQLLDRGPYVQEAPVIIDKPDIVYMVEKQPLSITVTLNHVNASVTWKRGGVTLSNRPGLFELSMPDDDQHTLKLCKVKSSDVGQLTCIANNKYGSDSCVLTLEMAVAPTFETIMEDLDVNVGETPRFAVVVEGKPVPDILWYKGDTLLSESSHFTFVYDDNECSLVVLNTQADDSGVYTCTAKNLAGSVSCKAELTVHTTKQYKDDPMEDEASILRKMRRLTDYYDVHKEIGRGAFSYVKRVIQKAGKLEYAAKFISARAKRKASALRELNILSHLDHERILYFHDAFEKKNAVIIITELCHEELLDRLTKKSTILESEIRSSVRQLLEGINYLHQLDILHLDIKPDNILMADHSSDQIRICDFGNAVKFMPDEAQYCKYGTPEFVAPEIVNQTPVSKATDIWPIGVLTYLCLTGVSPFAGENDRSSVLNIRNYNVAFEESMFTDLCHEAKGFVIKLLVADRLRPDANECLRHPWFKTLNKGKSISTESLKKFLSRRKWQRSLISYKSKMVMRSVPELLDDSSSHISIAVPRHLKKGSPPLSSSSDSDEDIDELPFIPMPLNVEFSGSRMSLTEIVGDDDMTGKLNGNAERPASLHPKSEPMEVEMSEREGGDPSARGRAKKRTTTEDEKGSSDEEPSQLAKRAELAKRPMRRGSSMESDEPEGGRRRGELRRGSSADSALQLHIKPEEGTEESPTDGSRILQKSVSMELPRRSPSPGAAKLSQEDYALKLELMRQRLLRGGSADNKMSGLRGPLLETLGMGDEKRFVRGSRLGNPPLVRAASSESPRDDIPKTKILRKSASFSQGDSEPMPLHRRIGAPLEIPLAQIEERRLQEAISMSALTEPVKDSRPITPKLQMPEEERHEKKKTEEKESVYKDNKPEEVIAVSKTEEEGRRVDQKSWDSDAQKKDQSKSEETREESEKVAEIEKKTSIVDETIEEEETRKDEEPEKETEPPTPKIEVKEYEGDVKVLPKSPEFIISTNSIMVTPTAAPASSPQSVSTYVTPSPPARTVLPDGRMSAYASIMQTIMVPSVPANTQSDTVPIPTPTSTPPSVPVYAPISRHISEPTLSSTSSSKSDLHQTTEHPAVFSRVASTEHPPKEPSPPKTPTRASPKREPSPGSNIQDLASEEVFEARFKKRESSLTRSLKRFSFQKTEDKPSTVSPETAEEMYRPGPTGAPLEFVPRRLEEKSKSVQDLREAEKDPGFMRRLSMRLKRTPSVEKQEEKLKEEEASAPRRRLSWALGRRGSQEKKEIEMMRLDGGPEPPPEPESKAPSESPILAMRKKIGSTMASLSTRIRSHSEDRKDDSEGKMETKRTPIFNKLRRSTSEGGSLKRMGIPQNQLAAQSGQGTSSESLDSMSSIQSESAIKNAELDRRSRWDRWGLTRGKKDKTVSQPDIPASIARENGSLRSRQYTRLTSDFPPVFHIKLRDHVLLEGDPVTLSCLPAGSPHPHISWMKDKKPLEIDPRMNMISCPDGRQLLMIMKTTKKDAGLYECVAANPLATVTSSCVVSLARLPNSPGTPEVPQKYKNTALVVWRPSDTTAPCTYSLERKTEGENNWLIVATGVADCYYNVLDLPAGASYRFRVACVNKAGQGPYSSLSEVVVLDSTEPQKSSGTVVVKTLSSAPAPAVVTSTMTLPAMKPAAIKPAPSTNSAPSTVQPVKPAPPPASSTPTYRPVPDIRTPSPGTPTPAKAKSTLNITMAKPSPASPAAQPQPQPPPKPSPPVILPKPQTPVSVVSPPSQTPPVSPPPLVSPPPIIGKPISSVPMYVPATTTSTPVTPVTPVTPVTPVTPNTPSPALSPPVVLVTSLSPVGEGTGTPNRMTPSGRATPSGRITPSGRRTPIGKPGDSTLRQGVPQKPYTFMDEKARGRFGVIRECRENATGNLYMAKIVPYEPESKQTVLQEYDILKSLHHEKIMALHEAYVTPRYLVLISECCSGKELLHSLIDRFRYSEDDVVAYIVQILQGLDYLHSRRILHLDIKPENIIVTYMNVVKIIDFGSAQTFNPLFLKQFSPPIGTLDYMSPEMLKGDVVGPPADIWSIGILTYIMLSGRLPFTENDPAETEARIQAAKFDLSKLYQNVSQSASLFIKKILCSYPWARPTIKDCFTNSWLQDAYLMRLRRQTLTFTTTRLKEFLEQQQHIRAQEATKHKVLLRSYQSNPQTPSTPQ

Target ORF information:

RefSeq Version	XM_009304896.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio SPEG complex locus b (spegb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304896.2

ORF Insert Sequence:

ATGTCTCAGA GTAGTGCTCA AGACTCTCGA CCCCAAACCC CTGTCAGTGA GTATTCCCGT 
AAGGAGCTTA CACTTCGGCC CTCACCAAAA CTGACAAGAG CTAGCTCCAA AATCTTTGAG 
AAGGTGCGAG TCTTCGAAGA GCGCAGAAGA AGCATTGATA ACCCTGAAGG ATCTATATCT 
GGACGTTCTT GGGCTGGATT TAACAGAGCT TCATCTATCG ACTCTGATGA AGGAGGGAGC 
AGGTTGGGCA TCTCCAGGGA GAGCTCCAAA GAAGACCTTC GTGAGGCTTT GAAGGCAGAT 
GCAGCCCAAC GAAGGACCAT GTTTAAACAG CGGGCTGCTT CCCTGGAGGA CAGACCTCGT 
TACACCCAGA AGGTGCAGGA CATTGAAAAC AAGTTCACAG AGGAGCTCCA GCGCATCAAA 
AAGCTTGTCG GCAAACCACA CATGAAGAAA TCCTTCTCAA CAGAGCAGCT CTCTACTTGT 
CACAGAAGCA GGCAGCCTGT AAGAAAGTTA GAGCCCATCC CACCTCAGGT CCTTCAAAAG 
CTGCAGGATA GAGAACGTGC TCAGCTAGCT CAGCTAGAGC AAGAGATGAG AGAGCGAGAC 
CAGGCCAAAG AACGATCACC TCCGAAATCA CCGTCTCGCA GAAGGAAAGA CCATCTGGCA 
CAACAACCAC CAGAAAGAGC TGAGACCAAG GTGGTAAGCA CAACTGGACT TGAACCATCC 
CCTCCAGAAG CCATGTCACT CTCTGATTTA CCAGGTCAAA GATCTCCAAG ATTTCGGGGC 
CCAAGCCCAA GCAGAGATAG TACACGAAGG TCTCCAACCG TAGAGATTAG TGCCATGGCT 
GAGGAGCGCC CTCGAGGAAG AGCTGAATCT CCTTCAAGAA ATGTTTTGGA GATGACGTTA 
CGCAAAGTGG AGCCAAGACC TGCAAGTCCA CTGGTAAAGA GGGTGGTTCG TGTTCAAGAA 
GTTCCACAAG CAAACGATCA GCCTATGCAT AGAAAGACGC CAATAGAGGT CTCGCTAAGA 
AAACTGGAAA GAAGGCCTGA AAGTCCCCTG GTGCAAGGAG AAACCGTAGC CATTCAAGAC 
TTCCCTGTTC AAGCTCCCCC CAAACCTCCA AGAGTTTCCT CATCTTCCTC GTCTGAGGAG 
AAGATGGAGC TGGAAGTGAC TCCTCCACCA CCCCCACTAA AACTCACTAT ACCAAAAATT 
ATTGTTGAAG AAGAGCCCAT GGAGACAGAC ACACCTGTTG AGAAGACTGA AAAGGCTGTG 
AAAGTTGGCA CTGGTAAAGA TGAAAAACAT CAAAGATCCA GAGGAAGAGG ACGCAGACAA 
AGACCAATGT CCCCTGAATT AGAATCTTCG GACGACTCCT ATGTGTCTGC AGGAGAGGAT 
CCACTAGAAG CTCCTGTGTT CGAGTTTCCA CTTCAGGACA CTGTGGCCTC CACTGGAACG 
GAGGTGCTAT TAAAATGCAT CATCAGTGGC ACCCCTCTCC CTGAAGTTAT ATGGAAGAAA 
GACAACACTG AGGTCAAGAA CAGTCCCACT CATGTTGTAA AGATAGAGGG GGAACGCCAC 
TCCTTGCTCA TCAAATGGAC AAAGCCAAGT GATGCAGGAA CCTACACAGT TACCGCTGTC 
AATGAGGTCG GTGAGGTGTC CAGCAGTGCT ACACTTTTCA TCAAACCAGA GCCCAGTCAG 
GACCATCGTG GTAACCTTGG TGTCCCTATG GATGTCAGCA GTCCCATCAC ATCTGATGAG 
GAGTATCTCA GTCCACTGGA AGAGAGCATG GACTTCTCCT CCTACAGGGG ACCGGAGCCA 
AGAAAGATCG TGGACACTCG ATTTAAGGAG CCTCCAGCCT TTCTGGCTGT TATTGGAGAC 
CAAAGCGTAA TAGAGGGTCA GGAGGTGACC ATGTCTGTTC TAGTGACCGG ACAACCTAAA 
CCTATGCTTT ACTGGCTGAG AGACAGAGTG ACCATTAAAA CCGGAACACG GCATATCGTC 
CAAGAGACAG AGGAGGGCAA CTTTGAAATG ATAATTAAAT CAGCACAGAG GTCAGACACC 
GGTGTTTACA CCTGCAAGAT CATCAATGAA TATGGAACTA AGCAATGTGA AGGCAAACTG 
GAGGTGAAAG CTCGTCCAGT AGAGCCAGGT CTGGCCATCA TTCGGCCAGT GAGGGACGTG 
ATGGTGAAGG CAGGGGAGAC CGCTCTGTTT GAGTGTCATG TGATCGGCCC GCAGGACACA 
GATGTTGACT GGCTGTCAGA TGGGAAACTC ATCCAGCCTG CTTTGTTGAA CTGCAAGATG 
CATTTTGATG GGAAAAGGTG CAGGCTGCTT CTAAACTCTG TACATGAGGA TGACAGTGGA 
ACGTACACCT GCAAACTGAG CACAGCTAAA GAGGAACTGA CCTCCAGTGC CAAGCTAAAA 
GTCATAGCAT CCATAGAGCC CCTTTTCACT CGTAAGCTGG ATGTGCTGGA AGTTATCGAG 
GGTCGTAATG CACGATTTGA CTGCAAGGTC AGCGGGACGC CGCCGCCTCA AGTCATATGG 
AGCCACTTCG ATCGTCCGCT CGAGGAAAAC GAAGATATCA GGATCTTAAA AGAAGGAGGA 
CGGCACTCAT TAATCATCTC TCATGTTAGT AATGAAGATG AGGGCCTCTA CACGGTTGCA 
GCGCGAAACT CACACGGGGA AGACGAATGT GCAGCGGAGC TCTATGTGCA GGAGCCCAGA 
CCAGCAATAT CTTCTCAAAT AGCGAAGCTG GAAAAAATGC CTTCCATTCC CGAAGAACCC 
GAAGTTCCTG AAAATGAGAT TGAGCGTTTC ACCATGCCTG ACTTCGTAAA GCCGCTTTAT 
GATATGGATG TAGTGGAGGG CAGAGAGGCT GTGCTCAGGT GCAAAGTGGC CGGCTTGCCG 
TACCCCACCA TAACATGGTT TCACAATGGC AAGAGAATTG ACAGCACGGA GGACAGAAAG 
ATGACACAGT TCAGAGATGT TCACAGCCTG GTGGTCCGCT GTGTTTGTCA TGCTCACGGA 
GGGGTTTACA AGTGTGTCAT ATCCAATAAA GTGGGCAAGG CCACCTGCTA TTCTCATCTT 
TACGTGACAG ACATCCTTCC TGACCCTCCA GATGGTGCCC CAGTCGTTGA GTCCATCACT 
GGCAAGACTA TCACGCTAAG CTGGAAGAAA CCCAAAAGAC TGGATCCGTC CATTGACCCC 
AGCTCCCTGA TGTATGCCAT TCAGCAACAG GCCCTGGGGT CGATCCAGTG GACCATCATC 
GCTTCTTGCC TGAAACAGAC CACCTATACC ATCAGCAATC TGTCTAAAGG TGTCCGCTAC 
GCCTTCAGGG TGCTATCAAT CACCAGCAAG GCCTTTAGCA AGCCGTCGCC TGCCACCGAG 
CCAATGCAGC TGCTAGATAG AGGTCCGTAC GTCCAGGAGG CTCCGGTAAT CATTGACAAG 
CCAGATATCG TGTATATGGT GGAGAAGCAG CCCTTGAGCA TCACAGTCAC TCTCAACCAC 
GTCAACGCAT CGGTCACATG GAAGAGAGGA GGAGTGACAT TATCTAACCG ACCGGGTCTT 
TTTGAGTTGA GCATGCCTGA TGATGACCAG CACACACTGA AGCTGTGTAA GGTGAAGAGC 
AGTGATGTTG GCCAGCTCAC CTGCATTGCC AACAACAAGT ACGGCAGCGA CTCCTGTGTC 
CTCACCCTGG AGATGGCAGT GGCACCAACT TTTGAGACAA TTATGGAAGA CCTGGATGTG 
AATGTAGGCG AGACTCCTCG ATTTGCAGTG GTGGTTGAAG GAAAGCCCGT CCCAGACATC 
CTCTGGTACA AGGGAGACAC CCTTCTGTCC GAAAGCAGCC ATTTCACATT TGTGTATGAT 
GACAATGAAT GTTCTCTAGT GGTGCTCAAC ACTCAAGCAG ACGACTCAGG AGTGTACACT 
TGTACTGCGA AGAATCTGGC CGGTTCTGTG TCCTGCAAGG CTGAGCTGAC CGTCCACACC 
ACCAAACAGT ACAAAGACGA CCCGATGGAG GATGAGGCGT CCATCCTCAG AAAGATGCGG 
AGGCTCACTG ATTACTATGA TGTGCACAAG GAAATTGGGA GAGGGGCTTT CTCATATGTG 
AAGCGGGTGA TACAGAAGGC AGGAAAGCTG GAATACGCAG CCAAGTTTAT TTCTGCTCGG 
GCTAAACGAA AGGCATCCGC TCTGAGAGAA CTGAACATCC TCTCTCATCT GGACCATGAG 
AGGATCCTCT ATTTTCATGA TGCCTTTGAG AAGAAGAATG CTGTCATCAT CATTACAGAG 
CTATGCCATG AGGAGCTTCT CGATAGGCTT ACAAAGAAAT CAACCATCTT GGAGTCCGAG 
ATCCGATCCA GCGTGAGGCA GCTGTTGGAG GGTATTAACT ATCTCCATCA GCTTGACATA 
CTTCATCTAG ACATCAAGCC TGATAATATC CTCATGGCAG ACCACAGTAG CGATCAGATC 
CGCATATGTG ATTTTGGAAA TGCTGTAAAG TTCATGCCTG ATGAGGCACA GTACTGCAAA 
TATGGCACTC CAGAATTTGT CGCCCCAGAG ATTGTTAACC AGACACCCGT CTCCAAGGCA 
ACGGACATCT GGCCCATTGG TGTTCTGACA TACTTATGTC TTACTGGAGT TTCTCCTTTT 
GCTGGGGAAA ACGACCGCAG CTCTGTTCTC AACATCAGGA ATTATAATGT GGCTTTTGAA 
GAAAGCATGT TTACAGACCT CTGCCATGAA GCCAAGGGAT TCGTCATCAA GCTGCTGGTG 
GCTGATAGAT TGAGGCCTGA TGCTAATGAA TGTCTTCGCC ACCCCTGGTT CAAGACCCTC 
AACAAGGGTA AAAGCATCAG TACAGAGTCC CTTAAAAAGT TTTTGTCCAG AAGAAAATGG 
CAGCGATCGC TTATCAGCTA CAAATCTAAA ATGGTGATGA GGTCTGTACC TGAGCTACTG 
GATGACTCCT CCAGCCATAT TTCCATTGCT GTTCCGCGAC ATCTGAAAAA AGGCTCACCA 
CCACTTTCAT CATCTTCAGA TTCAGATGAA GACATCGATG AGTTGCCTTT TATTCCCATG 
CCACTCAACG TGGAATTCTC AGGATCTAGA ATGTCACTGA CCGAAATTGT AGGTGATGAT 
GACATGACAG GTAAATTGAA TGGAAATGCT GAGAGGCCTG CTTCACTCCA TCCGAAGTCC 
GAGCCAATGG AAGTGGAGAT GTCAGAGAGA GAAGGTGGAG ATCCATCTGC TAGGGGTAGA 
GCCAAGAAGA GGACTACAAC AGAAGATGAA AAAGGGTCGT CTGATGAGGA ACCATCACAA 
CTGGCCAAAA GAGCAGAACT GGCAAAGAGA CCAATGCGCA GAGGTTCAAG CATGGAGTCA 
GATGAGCCAG AGGGAGGACG TCGCAGAGGA GAGTTACGCA GAGGGAGTTC GGCTGACAGT 
GCCTTACAGC TCCATATCAA ACCAGAGGAG GGCACAGAAG AAAGTCCTAC TGATGGAAGT 
AGAATTCTAC AAAAGTCTGT ATCTATGGAG CTTCCAAGGA GAAGCCCAAG TCCAGGTGCT 
GCAAAGCTTA GCCAGGAAGA CTATGCTCTG AAACTGGAAC TGATGAGGCA GCGACTACTC 
AGGGGTGGCT CTGCTGACAA CAAGATGAGT GGATTACGAG GCCCATTGCT GGAGACACTA 
GGTATGGGAG ATGAGAAGCG GTTTGTGCGG GGATCTCGTC TAGGTAACCC TCCACTTGTT 
AGAGCGGCAT CTAGTGAGTC ACCTAGAGAT GACATCCCAA AGACAAAGAT TCTCCGCAAG 
AGTGCTTCCT TTAGCCAGGG GGATTCAGAG CCCATGCCCC TGCACAGAAG AATCGGAGCA 
CCTCTAGAGA TTCCTCTAGC ACAAATAGAG GAGAGACGCC TACAGGAAGC AATATCCATG 
TCTGCTCTCA CAGAGCCTGT CAAAGATTCT CGCCCAATCA CTCCAAAACT TCAAATGCCT 
GAAGAAGAAA GACATGAAAA GAAGAAAACT GAAGAAAAAG AATCTGTCTA CAAGGACAAT 
AAACCTGAAG AGGTTATTGC TGTGTCAAAG ACTGAGGAAG AAGGAAGACG TGTTGATCAG 
AAATCTTGGG ATTCTGATGC ACAGAAAAAG GACCAGAGCA AGTCAGAGGA AACCAGGGAA 
GAGTCTGAAA AAGTGGCTGA AATTGAAAAA AAGACCTCTA TAGTGGACGA AACAATTGAG 
GAAGAGGAAA CAAGAAAAGA TGAGGAGCCA GAGAAAGAAA CTGAGCCTCC TACTCCAAAG 
ATTGAGGTTA AGGAATATGA GGGTGATGTA AAGGTATTAC CAAAGTCTCC AGAGTTTATC 
ATTTCCACAA ACTCAATCAT GGTGACCCCA ACAGCTGCCC CAGCATCCAG TCCCCAGAGT 
GTATCAACCT ATGTTACCCC ATCTCCCCCT GCACGCACAG TGCTCCCAGA TGGTAGGATG 
TCCGCATATG CCAGTATTAT GCAGACTATC ATGGTACCAT CTGTCCCAGC AAATACCCAA 
TCTGACACTG TTCCTATTCC TACACCAACT TCCACTCCAC CATCAGTTCC AGTCTATGCA 
CCCATTTCCA GACACATCTC AGAACCAACC CTTTCATCAA CTTCTTCATC CAAATCAGAT 
CTTCACCAAA CAACTGAGCA TCCTGCAGTC TTTTCTCGGG TTGCCTCCAC TGAACACCCT 
CCCAAAGAGC CAAGTCCTCC TAAGACACCC ACTCGCGCCT CCCCTAAGCG AGAGCCATCT 
CCTGGAAGCA ACATCCAGGA CCTTGCATCC GAGGAGGTTT TTGAAGCTCG CTTCAAGAAA 
CGTGAGTCTT CTCTAACTCG TAGTCTCAAA CGTTTCTCAT TTCAGAAGAC TGAAGACAAG 
CCCTCCACTG TTTCTCCTGA AACTGCTGAA GAAATGTATC GCCCTGGACC AACTGGTGCT 
CCTTTAGAGT TTGTGCCAAG GAGGTTGGAG GAGAAGTCCA AATCAGTACA AGATCTTCGA 
GAGGCAGAGA AGGACCCTGG GTTCATGAGG CGACTCTCCA TGCGCTTGAA AAGGACCCCA 
TCAGTGGAAA AGCAGGAGGA GAAGCTCAAA GAGGAGGAGG CTTCAGCACC CAGGCGTCGT 
CTTTCTTGGG CACTGGGACG GAGGGGATCC CAAGAGAAGA AGGAGATTGA GATGATGAGG 
TTGGATGGGG GTCCTGAGCC TCCCCCAGAG CCTGAGTCTA AAGCACCTTC TGAGTCACCT 
ATTCTTGCCA TGCGCAAGAA GATTGGCAGC ACCATGGCAA GCTTATCTAC AAGAATTAGG 
AGCCATTCTG AGGACAGGAA AGATGACAGT GAAGGCAAGA TGGAGACCAA GAGGACTCCT 
ATATTCAACA AACTAAGGCG TTCCACTTCT GAGGGGGGCA GTCTGAAGAG AATGGGTATC 
CCACAAAACC AACTGGCTGC TCAGTCAGGG CAAGGGACCT CATCAGAATC TCTTGATTCT 
ATGTCAAGCA TCCAGTCAGA ATCAGCTATT AAAAATGCAG AACTTGACCG CAGATCACGG 
TGGGACCGAT GGGGCTTGAC TCGAGGGAAA AAGGACAAGA CAGTTTCCCA ACCAGACATC 
CCAGCTTCCA TTGCTAGAGA GAATGGTTCT CTACGGTCTC GCCAATATAC ACGTCTAACA 
TCTGACTTCC CTCCGGTGTT CCATATCAAA CTGAGGGACC ATGTACTCTT GGAGGGAGAT 
CCTGTCACTC TCAGCTGCCT CCCTGCTGGC AGTCCTCACC CACACATCTC ATGGATGAAA 
GACAAGAAGC CACTTGAGAT TGACCCCAGG ATGAATATGA TATCTTGCCC CGATGGGAGA 
CAACTGCTGA TGATTATGAA GACCACCAAG AAGGATGCAG GCCTCTATGA ATGTGTGGCT 
GCAAACCCAC TGGCCACAGT CACCAGCTCA TGCGTTGTGT CCCTCGCTCG TCTGCCTAAT 
AGTCCTGGGA CACCCGAGGT TCCTCAGAAG TATAAAAACA CTGCTCTAGT GGTATGGAGA 
CCCTCAGACA CTACGGCACC CTGCACCTAC TCTCTGGAGA GGAAGACAGA GGGTGAGAAT 
AACTGGCTGA TAGTCGCCAC AGGTGTTGCT GACTGTTACT ATAACGTGTT GGATCTTCCG 
GCCGGCGCTT CATACAGGTT CAGAGTGGCC TGCGTCAATA AGGCTGGTCA GGGACCTTAC 
AGCAGCTTGT CTGAAGTAGT GGTCCTGGAC TCAACAGAGC CCCAGAAGTC CAGTGGCACA 
GTTGTAGTCA AAACGCTTTC ATCAGCTCCC GCTCCTGCAG TGGTAACGTC CACCATGACC 
CTTCCTGCAA TGAAGCCTGC TGCAATAAAA CCAGCACCAT CAACAAATTC AGCGCCCTCT 
ACAGTCCAAC CGGTAAAGCC TGCGCCTCCT CCTGCATCAT CAACACCTAC CTATCGTCCC 
GTGCCTGACA TTCGAACTCC ATCGCCTGGC ACTCCAACCC CTGCCAAAGC CAAAAGCACC 
CTCAACATTA CTATGGCCAA ACCCTCCCCA GCATCCCCTG CAGCTCAACC ACAACCACAA 
CCTCCACCCA AACCCTCGCC TCCGGTCATT CTGCCCAAAC CTCAGACCCC AGTCAGTGTG 
GTTTCACCTC CATCCCAAAC TCCTCCAGTG TCACCTCCAC CTCTAGTGTC TCCTCCTCCC 
ATTATTGGTA AACCAATCTC CTCAGTGCCC ATGTATGTCC CTGCTACAAC CACCTCGACT 
CCAGTGACTC CGGTCACTCC TGTCACCCCA GTCACTCCAG TCACCCCAAA TACGCCCTCT 
CCTGCCCTGT CGCCTCCAGT GGTGTTGGTT ACAAGTCTGA GTCCTGTAGG GGAGGGAACT 
GGGACACCCA ATCGAATGAC CCCTAGTGGA AGAGCCACGC CTTCTGGACG CATCACTCCA 
TCAGGCCGAA GGACCCCTAT TGGAAAGCCT GGAGACTCAA CGCTGAGGCA AGGAGTCCCT 
CAGAAACCTT ACACCTTCAT GGATGAGAAA GCAAGGGGTC GCTTTGGTGT GATCAGAGAG 
TGTCGAGAAA ATGCCACTGG TAACCTGTAC ATGGCTAAGA TTGTGCCATA TGAGCCAGAG 
AGCAAGCAGA CTGTATTACA GGAATACGAC ATCCTGAAGT CTCTCCATCA TGAGAAGATC 
ATGGCTCTCC ACGAGGCCTA CGTCACTCCA CGCTACCTGG TGCTCATCTC TGAGTGCTGC 
TCTGGCAAAG AACTACTCCA CAGTCTCATT GACAGGTTCC GATACTCTGA GGATGATGTG 
GTTGCATATA TCGTACAGAT CCTTCAGGGT CTGGACTACC TGCACAGCCG GAGGATCCTG 
CATCTCGACA TCAAACCAGA AAACATCATC GTCACTTACA TGAATGTGGT GAAGATTATC 
GATTTTGGTA GTGCACAGAC CTTCAATCCT CTGTTCCTGA AGCAGTTCTC CCCACCTATT 
GGAACACTGG ATTATATGTC TCCAGAGATG CTGAAAGGAG ATGTGGTGGG TCCACCAGCT 
GACATCTGGA GCATTGGCAT ATTGACTTAT ATCATGCTTA GCGGGAGGTT GCCGTTCACA 
GAAAATGACC CTGCCGAGAC CGAGGCCAGA ATACAGGCAG CCAAGTTTGA CCTCAGTAAG 
CTTTACCAAA ACGTGTCCCA AAGTGCCTCT CTGTTCATCA AAAAGATCCT GTGCAGCTAC 
CCTTGGGCCC GTCCGACCAT CAAGGACTGC TTTACCAACT CGTGGCTGCA GGACGCATAT 
CTGATGCGCC TGCGAAGACA GACTCTCACA TTCACCACCA CACGCCTCAA AGAGTTCCTG 
GAGCAGCAGC AGCACATCAG AGCCCAAGAG GCAACAAAGC ACAAAGTCCT CCTGCGTTCC 
TACCAGAGCA ACCCACAAAC CCCATCCACC CCTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spegb ( XM_021479011.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334686.1 Danio rerio SPEG complex locus b (spegb), transcript variant X7, mRNA.

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CloneID	ODa43993
Clone ID Related Accession (Same CDS sequence)	XM_021479011.1
Accession Version	XM_021479011.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8172bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	striated muscle preferentially expressed protein kinase isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGTCA GCAGTCCCAT CACATCTGAT GAGGAGTATC TCAGTCCACT GGAAGAGAGC 
ATGGACTTCT CCTCCTACAG GGGACCGGAG CCAAGAAAGA TCGTGGACAC TCGATTTAAG 
GAGCCTCCAG CCTTTCTGGC TGTTATTGGA GACCAAAGCG TAATAGAGGG TCAGGAGGTG 
ACCATGTCTG TTCTAGTGAC CGGACAACCT AAACCTATGC TTTACTGGCT GAGAGACAGA 
GTGACCATTA AAACCGGAAC ACGGCATATC GTCCAAGAGA CAGAGGAGGG CAACTTTGAA 
ATGATAATTA AATCAGCACA GAGGTCAGAC ACCGGTGTTT ACACCTGCAA GATCATCAAT 
GAATATGGAA CTAAGCAATG TGAAGGCAAA CTGGAGGTGA AAGCTCGTCC AGTAGAGCCA 
GGTCTGGCCA TCATTCGGCC AGTGAGGGAC GTGATGGTGA AGGCAGGGGA GACCGCTCTG 
TTTGAGTGTC ATGTGATCGG CCCGCAGGAC ACAGATGTTG ACTGGCTGTC AGATGGGAAA 
CTCATCCAGC CTGCTTTGTT GAACTGCAAG ATGCATTTTG ATGGGAAAAG GTGCAGGCTG 
CTTCTAAACT CTGTACATGA GGATGACAGT GGAACGTACA CCTGCAAACT GAGCACAGCT 
AAAGAGGAAC TGACCTCCAG TGCCAAGCTA AAAGTCATAG CATCCATAGA GCCCCTTTTC 
ACTCGTAAGC TGGATGTGCT GGAAGTTATC GAGGGTCGTA ATGCACGATT TGACTGCAAG 
GTCAGCGGGA CGCCGCCGCC TCAAGTCATA TGGAGCCACT TCGATCGTCC GCTCGAGGAA 
AACGAAGATA TCAGGATCTT AAAAGAAGGA GGACGGCACT CATTAATCAT CTCTCATGTT 
AGTAATGAAG ATGAGGGCCT CTACACGGTT GCAGCGCGAA ACTCACACGG GGAAGACGAA 
TGTGCAGCGG AGCTCTATGT GCAGGAGCCC AGACCAGCAA TATCTTCTCA AATAGCGAAG 
CTGGAAAAAA TGCCTTCCAT TCCCGAAGAA CCCGAAGTTC CTGAAAATGA GATTGAGCGT 
TTCACCATGC CTGACTTCGT AAAGCCGCTT TATGATATGG ATGTAGTGGA GGGCAGAGAG 
GCTGTGCTCA GGTGCAAAGT GGCCGGCTTG CCGTACCCCA CCATAACATG GTTTCACAAT 
GGCAAGAGAA TTGACAGCAC GGAGGACAGA AAGATGACAC AGTTCAGAGA TGTTCACAGC 
CTGGTGGTCC GCTGTGTTTG TCATGCTCAC GGAGGGGTTT ACAAGTGTGT CATATCCAAT 
AAAGTGGGCA AGGCCACCTG CTATTCTCAT CTTTACGTGA CAGACATCCT TCCTGACCCT 
CCAGATGGTG CCCCAGTCGT TGAGTCCATC ACTGGCAAGA CTATCACGCT AAGCTGGAAG 
AAACCCAAAA GACTGGATCC GTCCATTGAC CCCAGCTCCC TGATGTATGC CATTCAGCAA 
CAGGCCCTGG GGTCGATCCA GTGGACCATC ATCGCTTCTT GCCTGAAACA GACCACCTAT 
ACCATCAGCA ATCTGTCTAA AGGTGTCCGC TACGCCTTCA GGGTGCTATC AATCACCAGC 
AAGGCCTTTA GCAAGCCGTC GCCTGCCACC GAGCCAATGC AGCTGCTAGA TAGAGGTCCG 
TACGTCCAGG AGGCTCCGGT AATCATTGAC AAGCCAGATA TCGTGTATAT GGTGGAGAAG 
CAGCCCTTGA GCATCACAGT CACTCTCAAC CACGTCAACG CATCGGTCAC ATGGAAGAGA 
GGAGGAGTGA CATTATCTAA CCGACCGGGT CTTTTTGAGT TGAGCATGCC TGATGATGAC 
CAGCACACAC TGAAGCTGTG TAAGGTGAAG AGCAGTGATG TTGGCCAGCT CACCTGCATT 
GCCAACAACA AGTACGGCAG CGACTCCTGT GTCCTCACCC TGGAGATGGC AGTGGCACCA 
ACTTTTGAGA CAATTATGGA AGACCTGGAT GTGAATGTAG GCGAGACTCC TCGATTTGCA 
GTGGTGGTTG AAGGAAAGCC CGTCCCAGAC ATCCTCTGGT ACAAGGGAGA CACCCTTCTG 
TCCGAAAGCA GCCATTTCAC ATTTGTGTAT GATGACAATG AATGTTCTCT AGTGGTGCTC 
AACACTCAAG CAGACGACTC AGGAGTGTAC ACTTGTACTG CGAAGAATCT GGCCGGTTCT 
GTGTCCTGCA AGGCTGAGCT GACCGTCCAC ACCACCAAAC AGTACAAAGA CGACCCGATG 
GAGGATGAGG CGTCCATCCT CAGAAAGATG CGGAGGCTCA CTGATTACTA TGATGTGCAC 
AAGGAAATTG GGAGAGGGGC TTTCTCATAT GTGAAGCGGG TGATACAGAA GGCAGGAAAG 
CTGGAATACG CAGCCAAGTT TATTTCTGCT CGGGCTAAAC GAAAGGCATC CGCTCTGAGA 
GAACTGAACA TCCTCTCTCA TCTGGACCAT GAGAGGATCC TCTATTTTCA TGATGCCTTT 
GAGAAGAAGA ATGCTGTCAT CATCATTACA GAGCTATGCC ATGAGGAGCT TCTCGATAGG 
CTTACAAAGA AATCAACCAT CTTGGAGTCC GAGATCCGAT CCAGCGTGAG GCAGCTGTTG 
GAGGGTATTA ACTATCTCCA TCAGCTTGAC ATACTTCATC TAGACATCAA GCCTGATAAT 
ATCCTCATGG CAGACCACAG TAGCGATCAG ATCCGCATAT GTGATTTTGG AAATGCTGTA 
AAGTTCATGC CTGATGAGGC ACAGTACTGC AAATATGGCA CTCCAGAATT TGTCGCCCCA 
GAGATTGTTA ACCAGACACC CGTCTCCAAG GCAACGGACA TCTGGCCCAT TGGTGTTCTG 
ACATACTTAT GTCTTACTGG AGTTTCTCCT TTTGCTGGGG AAAACGACCG CAGCTCTGTT 
CTCAACATCA GGAATTATAA TGTGGCTTTT GAAGAAAGCA TGTTTACAGA CCTCTGCCAT 
GAAGCCAAGG GATTCGTCAT CAAGCTGCTG GTGGCTGATA GATTGAGGCC TGATGCTAAT 
GAATGTCTTC GCCACCCCTG GTTCAAGACC CTCAACAAGG GTAAAAGCAT CAGTACAGAG 
TCCCTTAAAA AGTTTTTGTC CAGAAGAAAA TGGCAGCGAT CGCTTATCAG CTACAAATCT 
AAAATGGTGA TGAGGTCTGT ACCTGAGCTA CTGGATGACT CCTCCAGCCA TATTTCCATT 
GCTGTTCCGC GACATCTGAA AAAAGGCTCA CCACCACTTT CATCATCTTC AGATTCAGAT 
GAAGACATCG ATGAGTTGCC TTTTATTCCC ATGCCACTCA ACGTGGAATT CTCAGGATCT 
AGAATGTCAC TGACCGAAAT TGTAGGTGAT GATGACATGA CAGGTAAATT GAATGGAAAT 
GCTGAGAGGC CTGCTTCACT CCATCCGAAG TCCGAGCCAA TGGAAGTGGA GATGTCAGAG 
AGAGAAGGTG GAGATCCATC TGCTAGGGGT AGAGCCAAGA AGAGGACTAC AACAGAAGAT 
GAAAAAGGGT CGTCTGATGA GGAACCATCA CAACTGGCCA AAAGAGCAGA ACTGGCAAAG 
AGACCAATGC GCAGAGGTTC AAGCATGGAG TCAGATGAGC CAGAGGGAGG ACGTCGCAGA 
GGAGAGTTAC GCAGAGGGAG TTCGGCTGAC AGTGCCTTAC AGCTCCATAT CAAACCAGAG 
GAGGGCACAG AAGAAAGTCC TACTGATGGA AGTAGAATTC TACAAAAGTC TGTATCTATG 
GAGCTTCCAA GGAGAAGCCC AAGTCCAGGT GCTGCAAAGC TTAGCCAGGA AGACTATGCT 
CTGAAACTGG AACTGATGAG GCAGCGACTA CTCAGGGGTG GCTCTGCTGA CAACAAGATG 
AGTGGATTAC GAGGCCCATT GCTGGAGACA CTAGGTATGG GAGATGAGAA GCGGTTTGTG 
CGGGGATCTC GTCTAGGTAA CCCTCCACTT GTTAGAGCGG CATCTAGTGA GTCACCTAGA 
GATGACATCC CAAAGACAAA GATTCTCCGC AAGAGTGCTT CCTTTAGCCA GGGGGATTCA 
GAGCCCATGC CCCTGCACAG AAGAATCGGA GCACCTCTAG AGATTCCTCT AGCACAAATA 
GAGGAGAGAC GCCTACAGGA AGCAATATCC ATGTCTGCTC TCACAGAGCC TGTCAAAGAT 
TCTCGCCCAA TCACTCCAAA ACTTCAAATG CCTGAAGAAG AAAGACATGA AAAGAAGAAA 
ACTGAAGAAA AAGAATCTGT CTACAAGGAC AATAAACCTG AAGAGGTTAT TGCTGTGTCA 
AAGACTGAGG AAGAAGGAAG ACGTGTTGAT CAGAAATCTT GGGATTCTGA TGCACAGAAA 
AAGGACCAGA GCAAGTCAGA GGAAACCAGG GAAGAGTCTG AAAAAGTGGC TGAAATTGAA 
AAAAAGACCT CTATAGTGGA CGAAACAATT GAGGAAGAGG AAACAAGAAA AGATGAGGAG 
CCAGAGAAAG AAACTGAGCC TCCTACTCCA AAGATTGAGG TTAAGGAATA TGAGGGTGAT 
GTAAAGGTAT TACCAAAGTC TCCAGAGTTT ATCATTTCCA CAAACTCAAT CATGGTGACC 
CCAACAGCTG CCCCAGCATC CAGTCCCCAG AGTGTATCAA CCTATGTTAC CCCATCTCCC 
CCTGCACGCA CAGTGCTCCC AGATGGTAGG ATGTCCGCAT ATGCCAGTAT TATGCAGACT 
ATCATGGTAC CATCTGTCCC AGCAAATACC CAATCTGACA CTGTTCCTAT TCCTACACCA 
ACTTCCACTC CACCATCAGT TCCAGTCTAT GCACCCATTT CCAGACACAT CTCAGAACCA 
ACCCTTTCAT CAACTTCTTC ATCCAAATCA GATCTTCACC AAACAACTGA GCATCCTGCA 
GTCTTTTCTC GGGTTGCCTC CACTGAACAC CCTCCCAAAG AGCCAAGTCC TCCTAAGACA 
CCCACTCGCG CCTCCCCTAA GCGAGAGCCA TCTCCTGGAA GCAACATCCA GGACCTTGCA 
TCCGAGGAGG TTTTTGAAGC TCGCTTCAAG AAACGTGAGT CTTCTCTAAC TCGTAGTCTC 
AAACGTTTCT CATTTCAGAA GACTGAAGAC AAGCCCTCCA CTGTTTCTCC TGAAACTGCT 
GAAGAAATGT ATCGCCCTGG ACCAACTGGT GCTCCTTTAG AGTTTGTGCC AAGGAGGTTG 
GAGGAGAAGT CCAAATCAGT ACAAGATCTT CGAGAGGCAG AGAAGGACCC TGGGTTCATG 
AGGCGACTCT CCATGCGCTT GAAAAGGACC CCATCAGTGG AAAAGCAGGA GGAGAAGCTC 
AAAGAGGAGG AGGCTTCAGC ACCCAGGCGT CGTCTTTCTT GGGCACTGGG ACGGAGGGGA 
TCCCAAGAGA AGAAGGAGAT TGAGATGATG AGGTTGGATG GGGGTCCTGA GCCTCCCCCA 
GAGCCTGAGT CTAAAGCACC TTCTGAGTCA CCTATTCTTG CCATGCGCAA GAAGATTGGC 
AGCACCATGG CAAGCTTATC TACAAGAATT AGGAGCCATT CTGAGGACAG GAAAGATGAC 
AGTGAAGGCA AGATGGAGAC CAAGAGGACT CCTATATTCA ACAAACTAAG GCGTTCCACT 
TCTGAGGGGG GCAGTCTGAA GAGAATGGGT ATCCCACAAA ACCAACTGGC TGCTCAGTCA 
GGGCAAGGGA CCTCATCAGA ATCTCTTGAT TCTATGTCAA GCATCCAGTC AGAATCAGCT 
ATTAAAAATG CAGAACTTGA CCGCAGATCA CGGTGGGACC GATGGGGCTT GACTCGAGGG 
AAAAAGGACA AGACAGTTTC CCAACCAGAC ATCCCAGCTT CCATTGCTAG AGAGAATGGT 
TCTCTACGGT CTCGCCAATA TACACGTCTA ACATCTGACT TCCCTCCGGT GTTCCATATC 
AAACTGAGGG ACCATGTACT CTTGGAGGGA GATCCTGTCA CTCTCAGCTG CCTCCCTGCT 
GGCAGTCCTC ACCCACACAT CTCATGGATG AAAGACAAGA AGCCACTTGA GATTGACCCC 
AGGATGAATA TGATATCTTG CCCCGATGGG AGACAACTGC TGATGATTAT GAAGACCACC 
AAGAAGGATG CAGGCCTCTA TGAATGTGTG GCTGCAAACC CACTGGCCAC AGTCACCAGC 
TCATGCGTTG TGTCCCTCGC TCGTCTGCCT AATAGTCCTG GGACACCCGA GGTTCCTCAG 
AAGTATAAAA ACACTGCTCT AGTGGTATGG AGACCCTCAG ACACTACGGC ACCCTGCACC 
TACTCTCTGG AGAGGAAGAC AGAGGGTGAG AATAACTGGC TGATAGTCGC CACAGGTGTT 
GCTGACTGTT ACTATAACGT GTTGGATCTT CCGGCCGGCG CTTCATACAG GTTCAGAGTG 
GCCTGCGTCA ATAAGGCTGG TCAGGGACCT TACAGCAGCT TGTCTGAAGT AGTGGTCCTG 
GACTCAACAG AGCCCCAGAA GTCCAGTGGC ACAGTTGTAG TCAAAACGCT TTCATCAGCT 
CCCGCTCCTG CAGTGGTAAC GTCCACCATG ACCCTTCCTG CAATGAAGCC TGCTGCAATA 
AAACCAGCAC CATCAACAAA TTCAGCGCCC TCTACAGTCC AACCGGTAAA GCCTGCGCCT 
CCTCCTGCAT CATCAACACC TACCTATCGT CCCGTGCCTG ACATTCGAAC TCCATCGCCT 
GGCACTCCAA CCCCTGCCAA AGCCAAAAGC ACCCTCAACA TTACTATGGC CAAACCCTCC 
CCAGCATCCC CTGCAGCTCA ACCACAACCA CAACCTCCAC CCAAACCCTC GCCTCCGGTC 
ATTCTGCCCA AACCTCAGAC CCCAGTCAGT GTGGTTTCAC CTCCATCCCA AACTCCTCCA 
GTGTCACCTC CACCTCTAGT GTCTCCTCCT CCCATTATTG GTAAACCAAT CTCCTCAGTG 
CCCATGTATG TCCCTGCTAC AACCACCTCG ACTCCAGTGA CTCCGGTCAC TCCTGTCACC 
CCAGTCACTC CAGTCACCCC AAATACGCCC TCTCCTGCCC TGTCGCCTCC AGTGGTGTTG 
GTTACAAGTC TGAGTCCTGT AGGGGAGGGA ACTGGGACAC CCAATCGAAT GACCCCTAGT 
GGAAGAGCCA CGCCTTCTGG ACGCATCACT CCATCAGGCC GAAGGACCCC TATTGGAAAG 
CCTGGAGACT CAACGCTGAG GCAAGGAGTC CCTCAGAAAC CTTACACCTT CATGGATGAG 
AAAGCAAGGG GTCGCTTTGG TGTGATCAGA GAGTGTCGAG AAAATGCCAC TGGTAACCTG 
TACATGGCTA AGATTGTGCC ATATGAGCCA GAGAGCAAGC AGACTGTATT ACAGGAATAC 
GACATCCTGA AGTCTCTCCA TCATGAGAAG ATCATGGCTC TCCACGAGGC CTACGTCACT 
CCACGCTACC TGGTGCTCAT CTCTGAGTGC TGCTCTGGCA AAGAACTACT CCACAGTCTC 
ATTGACAGGT TCCGATACTC TGAGGATGAT GTGGTTGCAT ATATCGTACA GATCCTTCAG 
GGTCTGGACT ACCTGCACAG CCGGAGGATC CTGCATCTCG ACATCAAACC AGAAAACATC 
ATCGTCACTT ACATGAATGT GGTGAAGATT ATCGATTTTG GTAGTGCACA GACCTTCAAT 
CCTCTGTTCC TGAAGCAGTT CTCCCCACCT ATTGGAACAC TGGATTATAT GTCTCCAGAG 
ATGCTGAAAG GAGATGTGGT GGGTCCACCA GCTGACATCT GGAGCATTGG CATATTGACT 
TATATCATGC TTAGCGGGAG GTTGCCGTTC ACAGAAAATG ACCCTGCCGA GACCGAGGCC 
AGAATACAGG CAGCCAAGTT TGACCTCAGT AAGCTTTACC AAAACGTGTC CCAAAGTGCC 
TCTCTGTTCA TCAAAAAGAT CCTGTGCAGC TACCCTTGGG CCCGTCCGAC CATCAAGGAC 
TGCTTTACCA ACTCGTGGCT GCAGGACGCA TATCTGATGC GCCTGCGAAG ACAGACTCTC 
ACATTCACCA CCACACGCCT CAAAGAGTTC CTGGAGCAGC AGCAGCACAT CAGAGCCCAA 
GAGGCAACAA AGCACAAAGT CCTCCTGCGT TCCTACCAGA GCAACCCACA AACCCCATCC 
ACCCCTCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334686.1
CDS	212..8383
Translation	MDVSSPITSDEEYLSPLEESMDFSSYRGPEPRKIVDTRFKEPPAFLAVIGDQSVIEGQEVTMSVLVTGQPKPMLYWLRDRVTIKTGTRHIVQETEEGNFEMIIKSAQRSDTGVYTCKIINEYGTKQCEGKLEVKARPVEPGLAIIRPVRDVMVKAGETALFECHVIGPQDTDVDWLSDGKLIQPALLNCKMHFDGKRCRLLLNSVHEDDSGTYTCKLSTAKEELTSSAKLKVIASIEPLFTRKLDVLEVIEGRNARFDCKVSGTPPPQVIWSHFDRPLEENEDIRILKEGGRHSLIISHVSNEDEGLYTVAARNSHGEDECAAELYVQEPRPAISSQIAKLEKMPSIPEEPEVPENEIERFTMPDFVKPLYDMDVVEGREAVLRCKVAGLPYPTITWFHNGKRIDSTEDRKMTQFRDVHSLVVRCVCHAHGGVYKCVISNKVGKATCYSHLYVTDILPDPPDGAPVVESITGKTITLSWKKPKRLDPSIDPSSLMYAIQQQALGSIQWTIIASCLKQTTYTISNLSKGVRYAFRVLSITSKAFSKPSPATEPMQLLDRGPYVQEAPVIIDKPDIVYMVEKQPLSITVTLNHVNASVTWKRGGVTLSNRPGLFELSMPDDDQHTLKLCKVKSSDVGQLTCIANNKYGSDSCVLTLEMAVAPTFETIMEDLDVNVGETPRFAVVVEGKPVPDILWYKGDTLLSESSHFTFVYDDNECSLVVLNTQADDSGVYTCTAKNLAGSVSCKAELTVHTTKQYKDDPMEDEASILRKMRRLTDYYDVHKEIGRGAFSYVKRVIQKAGKLEYAAKFISARAKRKASALRELNILSHLDHERILYFHDAFEKKNAVIIITELCHEELLDRLTKKSTILESEIRSSVRQLLEGINYLHQLDILHLDIKPDNILMADHSSDQIRICDFGNAVKFMPDEAQYCKYGTPEFVAPEIVNQTPVSKATDIWPIGVLTYLCLTGVSPFAGENDRSSVLNIRNYNVAFEESMFTDLCHEAKGFVIKLLVADRLRPDANECLRHPWFKTLNKGKSISTESLKKFLSRRKWQRSLISYKSKMVMRSVPELLDDSSSHISIAVPRHLKKGSPPLSSSSDSDEDIDELPFIPMPLNVEFSGSRMSLTEIVGDDDMTGKLNGNAERPASLHPKSEPMEVEMSEREGGDPSARGRAKKRTTTEDEKGSSDEEPSQLAKRAELAKRPMRRGSSMESDEPEGGRRRGELRRGSSADSALQLHIKPEEGTEESPTDGSRILQKSVSMELPRRSPSPGAAKLSQEDYALKLELMRQRLLRGGSADNKMSGLRGPLLETLGMGDEKRFVRGSRLGNPPLVRAASSESPRDDIPKTKILRKSASFSQGDSEPMPLHRRIGAPLEIPLAQIEERRLQEAISMSALTEPVKDSRPITPKLQMPEEERHEKKKTEEKESVYKDNKPEEVIAVSKTEEEGRRVDQKSWDSDAQKKDQSKSEETREESEKVAEIEKKTSIVDETIEEEETRKDEEPEKETEPPTPKIEVKEYEGDVKVLPKSPEFIISTNSIMVTPTAAPASSPQSVSTYVTPSPPARTVLPDGRMSAYASIMQTIMVPSVPANTQSDTVPIPTPTSTPPSVPVYAPISRHISEPTLSSTSSSKSDLHQTTEHPAVFSRVASTEHPPKEPSPPKTPTRASPKREPSPGSNIQDLASEEVFEARFKKRESSLTRSLKRFSFQKTEDKPSTVSPETAEEMYRPGPTGAPLEFVPRRLEEKSKSVQDLREAEKDPGFMRRLSMRLKRTPSVEKQEEKLKEEEASAPRRRLSWALGRRGSQEKKEIEMMRLDGGPEPPPEPESKAPSESPILAMRKKIGSTMASLSTRIRSHSEDRKDDSEGKMETKRTPIFNKLRRSTSEGGSLKRMGIPQNQLAAQSGQGTSSESLDSMSSIQSESAIKNAELDRRSRWDRWGLTRGKKDKTVSQPDIPASIARENGSLRSRQYTRLTSDFPPVFHIKLRDHVLLEGDPVTLSCLPAGSPHPHISWMKDKKPLEIDPRMNMISCPDGRQLLMIMKTTKKDAGLYECVAANPLATVTSSCVVSLARLPNSPGTPEVPQKYKNTALVVWRPSDTTAPCTYSLERKTEGENNWLIVATGVADCYYNVLDLPAGASYRFRVACVNKAGQGPYSSLSEVVVLDSTEPQKSSGTVVVKTLSSAPAPAVVTSTMTLPAMKPAAIKPAPSTNSAPSTVQPVKPAPPPASSTPTYRPVPDIRTPSPGTPTPAKAKSTLNITMAKPSPASPAAQPQPQPPPKPSPPVILPKPQTPVSVVSPPSQTPPVSPPPLVSPPPIIGKPISSVPMYVPATTTSTPVTPVTPVTPVTPVTPNTPSPALSPPVVLVTSLSPVGEGTGTPNRMTPSGRATPSGRITPSGRRTPIGKPGDSTLRQGVPQKPYTFMDEKARGRFGVIRECRENATGNLYMAKIVPYEPESKQTVLQEYDILKSLHHEKIMALHEAYVTPRYLVLISECCSGKELLHSLIDRFRYSEDDVVAYIVQILQGLDYLHSRRILHLDIKPENIIVTYMNVVKIIDFGSAQTFNPLFLKQFSPPIGTLDYMSPEMLKGDVVGPPADIWSIGILTYIMLSGRLPFTENDPAETEARIQAAKFDLSKLYQNVSQSASLFIKKILCSYPWARPTIKDCFTNSWLQDAYLMRLRRQTLTFTTTRLKEFLEQQQHIRAQEATKHKVLLRSYQSNPQTPSTPQ

Target ORF information:

RefSeq Version	XM_021479011.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SPEG complex locus b (spegb), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479011.1

ORF Insert Sequence:

ATGGATGTCA GCAGTCCCAT CACATCTGAT GAGGAGTATC TCAGTCCACT GGAAGAGAGC 
ATGGACTTCT CCTCCTACAG GGGACCGGAG CCAAGAAAGA TCGTGGACAC TCGATTTAAG 
GAGCCTCCAG CCTTTCTGGC TGTTATTGGA GACCAAAGCG TAATAGAGGG TCAGGAGGTG 
ACCATGTCTG TTCTAGTGAC CGGACAACCT AAACCTATGC TTTACTGGCT GAGAGACAGA 
GTGACCATTA AAACCGGAAC ACGGCATATC GTCCAAGAGA CAGAGGAGGG CAACTTTGAA 
ATGATAATTA AATCAGCACA GAGGTCAGAC ACCGGTGTTT ACACCTGCAA GATCATCAAT 
GAATATGGAA CTAAGCAATG TGAAGGCAAA CTGGAGGTGA AAGCTCGTCC AGTAGAGCCA 
GGTCTGGCCA TCATTCGGCC AGTGAGGGAC GTGATGGTGA AGGCAGGGGA GACCGCTCTG 
TTTGAGTGTC ATGTGATCGG CCCGCAGGAC ACAGATGTTG ACTGGCTGTC AGATGGGAAA 
CTCATCCAGC CTGCTTTGTT GAACTGCAAG ATGCATTTTG ATGGGAAAAG GTGCAGGCTG 
CTTCTAAACT CTGTACATGA GGATGACAGT GGAACGTACA CCTGCAAACT GAGCACAGCT 
AAAGAGGAAC TGACCTCCAG TGCCAAGCTA AAAGTCATAG CATCCATAGA GCCCCTTTTC 
ACTCGTAAGC TGGATGTGCT GGAAGTTATC GAGGGTCGTA ATGCACGATT TGACTGCAAG 
GTCAGCGGGA CGCCGCCGCC TCAAGTCATA TGGAGCCACT TCGATCGTCC GCTCGAGGAA 
AACGAAGATA TCAGGATCTT AAAAGAAGGA GGACGGCACT CATTAATCAT CTCTCATGTT 
AGTAATGAAG ATGAGGGCCT CTACACGGTT GCAGCGCGAA ACTCACACGG GGAAGACGAA 
TGTGCAGCGG AGCTCTATGT GCAGGAGCCC AGACCAGCAA TATCTTCTCA AATAGCGAAG 
CTGGAAAAAA TGCCTTCCAT TCCCGAAGAA CCCGAAGTTC CTGAAAATGA GATTGAGCGT 
TTCACCATGC CTGACTTCGT AAAGCCGCTT TATGATATGG ATGTAGTGGA GGGCAGAGAG 
GCTGTGCTCA GGTGCAAAGT GGCCGGCTTG CCGTACCCCA CCATAACATG GTTTCACAAT 
GGCAAGAGAA TTGACAGCAC GGAGGACAGA AAGATGACAC AGTTCAGAGA TGTTCACAGC 
CTGGTGGTCC GCTGTGTTTG TCATGCTCAC GGAGGGGTTT ACAAGTGTGT CATATCCAAT 
AAAGTGGGCA AGGCCACCTG CTATTCTCAT CTTTACGTGA CAGACATCCT TCCTGACCCT 
CCAGATGGTG CCCCAGTCGT TGAGTCCATC ACTGGCAAGA CTATCACGCT AAGCTGGAAG 
AAACCCAAAA GACTGGATCC GTCCATTGAC CCCAGCTCCC TGATGTATGC CATTCAGCAA 
CAGGCCCTGG GGTCGATCCA GTGGACCATC ATCGCTTCTT GCCTGAAACA GACCACCTAT 
ACCATCAGCA ATCTGTCTAA AGGTGTCCGC TACGCCTTCA GGGTGCTATC AATCACCAGC 
AAGGCCTTTA GCAAGCCGTC GCCTGCCACC GAGCCAATGC AGCTGCTAGA TAGAGGTCCG 
TACGTCCAGG AGGCTCCGGT AATCATTGAC AAGCCAGATA TCGTGTATAT GGTGGAGAAG 
CAGCCCTTGA GCATCACAGT CACTCTCAAC CACGTCAACG CATCGGTCAC ATGGAAGAGA 
GGAGGAGTGA CATTATCTAA CCGACCGGGT CTTTTTGAGT TGAGCATGCC TGATGATGAC 
CAGCACACAC TGAAGCTGTG TAAGGTGAAG AGCAGTGATG TTGGCCAGCT CACCTGCATT 
GCCAACAACA AGTACGGCAG CGACTCCTGT GTCCTCACCC TGGAGATGGC AGTGGCACCA 
ACTTTTGAGA CAATTATGGA AGACCTGGAT GTGAATGTAG GCGAGACTCC TCGATTTGCA 
GTGGTGGTTG AAGGAAAGCC CGTCCCAGAC ATCCTCTGGT ACAAGGGAGA CACCCTTCTG 
TCCGAAAGCA GCCATTTCAC ATTTGTGTAT GATGACAATG AATGTTCTCT AGTGGTGCTC 
AACACTCAAG CAGACGACTC AGGAGTGTAC ACTTGTACTG CGAAGAATCT GGCCGGTTCT 
GTGTCCTGCA AGGCTGAGCT GACCGTCCAC ACCACCAAAC AGTACAAAGA CGACCCGATG 
GAGGATGAGG CGTCCATCCT CAGAAAGATG CGGAGGCTCA CTGATTACTA TGATGTGCAC 
AAGGAAATTG GGAGAGGGGC TTTCTCATAT GTGAAGCGGG TGATACAGAA GGCAGGAAAG 
CTGGAATACG CAGCCAAGTT TATTTCTGCT CGGGCTAAAC GAAAGGCATC CGCTCTGAGA 
GAACTGAACA TCCTCTCTCA TCTGGACCAT GAGAGGATCC TCTATTTTCA TGATGCCTTT 
GAGAAGAAGA ATGCTGTCAT CATCATTACA GAGCTATGCC ATGAGGAGCT TCTCGATAGG 
CTTACAAAGA AATCAACCAT CTTGGAGTCC GAGATCCGAT CCAGCGTGAG GCAGCTGTTG 
GAGGGTATTA ACTATCTCCA TCAGCTTGAC ATACTTCATC TAGACATCAA GCCTGATAAT 
ATCCTCATGG CAGACCACAG TAGCGATCAG ATCCGCATAT GTGATTTTGG AAATGCTGTA 
AAGTTCATGC CTGATGAGGC ACAGTACTGC AAATATGGCA CTCCAGAATT TGTCGCCCCA 
GAGATTGTTA ACCAGACACC CGTCTCCAAG GCAACGGACA TCTGGCCCAT TGGTGTTCTG 
ACATACTTAT GTCTTACTGG AGTTTCTCCT TTTGCTGGGG AAAACGACCG CAGCTCTGTT 
CTCAACATCA GGAATTATAA TGTGGCTTTT GAAGAAAGCA TGTTTACAGA CCTCTGCCAT 
GAAGCCAAGG GATTCGTCAT CAAGCTGCTG GTGGCTGATA GATTGAGGCC TGATGCTAAT 
GAATGTCTTC GCCACCCCTG GTTCAAGACC CTCAACAAGG GTAAAAGCAT CAGTACAGAG 
TCCCTTAAAA AGTTTTTGTC CAGAAGAAAA TGGCAGCGAT CGCTTATCAG CTACAAATCT 
AAAATGGTGA TGAGGTCTGT ACCTGAGCTA CTGGATGACT CCTCCAGCCA TATTTCCATT 
GCTGTTCCGC GACATCTGAA AAAAGGCTCA CCACCACTTT CATCATCTTC AGATTCAGAT 
GAAGACATCG ATGAGTTGCC TTTTATTCCC ATGCCACTCA ACGTGGAATT CTCAGGATCT 
AGAATGTCAC TGACCGAAAT TGTAGGTGAT GATGACATGA CAGGTAAATT GAATGGAAAT 
GCTGAGAGGC CTGCTTCACT CCATCCGAAG TCCGAGCCAA TGGAAGTGGA GATGTCAGAG 
AGAGAAGGTG GAGATCCATC TGCTAGGGGT AGAGCCAAGA AGAGGACTAC AACAGAAGAT 
GAAAAAGGGT CGTCTGATGA GGAACCATCA CAACTGGCCA AAAGAGCAGA ACTGGCAAAG 
AGACCAATGC GCAGAGGTTC AAGCATGGAG TCAGATGAGC CAGAGGGAGG ACGTCGCAGA 
GGAGAGTTAC GCAGAGGGAG TTCGGCTGAC AGTGCCTTAC AGCTCCATAT CAAACCAGAG 
GAGGGCACAG AAGAAAGTCC TACTGATGGA AGTAGAATTC TACAAAAGTC TGTATCTATG 
GAGCTTCCAA GGAGAAGCCC AAGTCCAGGT GCTGCAAAGC TTAGCCAGGA AGACTATGCT 
CTGAAACTGG AACTGATGAG GCAGCGACTA CTCAGGGGTG GCTCTGCTGA CAACAAGATG 
AGTGGATTAC GAGGCCCATT GCTGGAGACA CTAGGTATGG GAGATGAGAA GCGGTTTGTG 
CGGGGATCTC GTCTAGGTAA CCCTCCACTT GTTAGAGCGG CATCTAGTGA GTCACCTAGA 
GATGACATCC CAAAGACAAA GATTCTCCGC AAGAGTGCTT CCTTTAGCCA GGGGGATTCA 
GAGCCCATGC CCCTGCACAG AAGAATCGGA GCACCTCTAG AGATTCCTCT AGCACAAATA 
GAGGAGAGAC GCCTACAGGA AGCAATATCC ATGTCTGCTC TCACAGAGCC TGTCAAAGAT 
TCTCGCCCAA TCACTCCAAA ACTTCAAATG CCTGAAGAAG AAAGACATGA AAAGAAGAAA 
ACTGAAGAAA AAGAATCTGT CTACAAGGAC AATAAACCTG AAGAGGTTAT TGCTGTGTCA 
AAGACTGAGG AAGAAGGAAG ACGTGTTGAT CAGAAATCTT GGGATTCTGA TGCACAGAAA 
AAGGACCAGA GCAAGTCAGA GGAAACCAGG GAAGAGTCTG AAAAAGTGGC TGAAATTGAA 
AAAAAGACCT CTATAGTGGA CGAAACAATT GAGGAAGAGG AAACAAGAAA AGATGAGGAG 
CCAGAGAAAG AAACTGAGCC TCCTACTCCA AAGATTGAGG TTAAGGAATA TGAGGGTGAT 
GTAAAGGTAT TACCAAAGTC TCCAGAGTTT ATCATTTCCA CAAACTCAAT CATGGTGACC 
CCAACAGCTG CCCCAGCATC CAGTCCCCAG AGTGTATCAA CCTATGTTAC CCCATCTCCC 
CCTGCACGCA CAGTGCTCCC AGATGGTAGG ATGTCCGCAT ATGCCAGTAT TATGCAGACT 
ATCATGGTAC CATCTGTCCC AGCAAATACC CAATCTGACA CTGTTCCTAT TCCTACACCA 
ACTTCCACTC CACCATCAGT TCCAGTCTAT GCACCCATTT CCAGACACAT CTCAGAACCA 
ACCCTTTCAT CAACTTCTTC ATCCAAATCA GATCTTCACC AAACAACTGA GCATCCTGCA 
GTCTTTTCTC GGGTTGCCTC CACTGAACAC CCTCCCAAAG AGCCAAGTCC TCCTAAGACA 
CCCACTCGCG CCTCCCCTAA GCGAGAGCCA TCTCCTGGAA GCAACATCCA GGACCTTGCA 
TCCGAGGAGG TTTTTGAAGC TCGCTTCAAG AAACGTGAGT CTTCTCTAAC TCGTAGTCTC 
AAACGTTTCT CATTTCAGAA GACTGAAGAC AAGCCCTCCA CTGTTTCTCC TGAAACTGCT 
GAAGAAATGT ATCGCCCTGG ACCAACTGGT GCTCCTTTAG AGTTTGTGCC AAGGAGGTTG 
GAGGAGAAGT CCAAATCAGT ACAAGATCTT CGAGAGGCAG AGAAGGACCC TGGGTTCATG 
AGGCGACTCT CCATGCGCTT GAAAAGGACC CCATCAGTGG AAAAGCAGGA GGAGAAGCTC 
AAAGAGGAGG AGGCTTCAGC ACCCAGGCGT CGTCTTTCTT GGGCACTGGG ACGGAGGGGA 
TCCCAAGAGA AGAAGGAGAT TGAGATGATG AGGTTGGATG GGGGTCCTGA GCCTCCCCCA 
GAGCCTGAGT CTAAAGCACC TTCTGAGTCA CCTATTCTTG CCATGCGCAA GAAGATTGGC 
AGCACCATGG CAAGCTTATC TACAAGAATT AGGAGCCATT CTGAGGACAG GAAAGATGAC 
AGTGAAGGCA AGATGGAGAC CAAGAGGACT CCTATATTCA ACAAACTAAG GCGTTCCACT 
TCTGAGGGGG GCAGTCTGAA GAGAATGGGT ATCCCACAAA ACCAACTGGC TGCTCAGTCA 
GGGCAAGGGA CCTCATCAGA ATCTCTTGAT TCTATGTCAA GCATCCAGTC AGAATCAGCT 
ATTAAAAATG CAGAACTTGA CCGCAGATCA CGGTGGGACC GATGGGGCTT GACTCGAGGG 
AAAAAGGACA AGACAGTTTC CCAACCAGAC ATCCCAGCTT CCATTGCTAG AGAGAATGGT 
TCTCTACGGT CTCGCCAATA TACACGTCTA ACATCTGACT TCCCTCCGGT GTTCCATATC 
AAACTGAGGG ACCATGTACT CTTGGAGGGA GATCCTGTCA CTCTCAGCTG CCTCCCTGCT 
GGCAGTCCTC ACCCACACAT CTCATGGATG AAAGACAAGA AGCCACTTGA GATTGACCCC 
AGGATGAATA TGATATCTTG CCCCGATGGG AGACAACTGC TGATGATTAT GAAGACCACC 
AAGAAGGATG CAGGCCTCTA TGAATGTGTG GCTGCAAACC CACTGGCCAC AGTCACCAGC 
TCATGCGTTG TGTCCCTCGC TCGTCTGCCT AATAGTCCTG GGACACCCGA GGTTCCTCAG 
AAGTATAAAA ACACTGCTCT AGTGGTATGG AGACCCTCAG ACACTACGGC ACCCTGCACC 
TACTCTCTGG AGAGGAAGAC AGAGGGTGAG AATAACTGGC TGATAGTCGC CACAGGTGTT 
GCTGACTGTT ACTATAACGT GTTGGATCTT CCGGCCGGCG CTTCATACAG GTTCAGAGTG 
GCCTGCGTCA ATAAGGCTGG TCAGGGACCT TACAGCAGCT TGTCTGAAGT AGTGGTCCTG 
GACTCAACAG AGCCCCAGAA GTCCAGTGGC ACAGTTGTAG TCAAAACGCT TTCATCAGCT 
CCCGCTCCTG CAGTGGTAAC GTCCACCATG ACCCTTCCTG CAATGAAGCC TGCTGCAATA 
AAACCAGCAC CATCAACAAA TTCAGCGCCC TCTACAGTCC AACCGGTAAA GCCTGCGCCT 
CCTCCTGCAT CATCAACACC TACCTATCGT CCCGTGCCTG ACATTCGAAC TCCATCGCCT 
GGCACTCCAA CCCCTGCCAA AGCCAAAAGC ACCCTCAACA TTACTATGGC CAAACCCTCC 
CCAGCATCCC CTGCAGCTCA ACCACAACCA CAACCTCCAC CCAAACCCTC GCCTCCGGTC 
ATTCTGCCCA AACCTCAGAC CCCAGTCAGT GTGGTTTCAC CTCCATCCCA AACTCCTCCA 
GTGTCACCTC CACCTCTAGT GTCTCCTCCT CCCATTATTG GTAAACCAAT CTCCTCAGTG 
CCCATGTATG TCCCTGCTAC AACCACCTCG ACTCCAGTGA CTCCGGTCAC TCCTGTCACC 
CCAGTCACTC CAGTCACCCC AAATACGCCC TCTCCTGCCC TGTCGCCTCC AGTGGTGTTG 
GTTACAAGTC TGAGTCCTGT AGGGGAGGGA ACTGGGACAC CCAATCGAAT GACCCCTAGT 
GGAAGAGCCA CGCCTTCTGG ACGCATCACT CCATCAGGCC GAAGGACCCC TATTGGAAAG 
CCTGGAGACT CAACGCTGAG GCAAGGAGTC CCTCAGAAAC CTTACACCTT CATGGATGAG 
AAAGCAAGGG GTCGCTTTGG TGTGATCAGA GAGTGTCGAG AAAATGCCAC TGGTAACCTG 
TACATGGCTA AGATTGTGCC ATATGAGCCA GAGAGCAAGC AGACTGTATT ACAGGAATAC 
GACATCCTGA AGTCTCTCCA TCATGAGAAG ATCATGGCTC TCCACGAGGC CTACGTCACT 
CCACGCTACC TGGTGCTCAT CTCTGAGTGC TGCTCTGGCA AAGAACTACT CCACAGTCTC 
ATTGACAGGT TCCGATACTC TGAGGATGAT GTGGTTGCAT ATATCGTACA GATCCTTCAG 
GGTCTGGACT ACCTGCACAG CCGGAGGATC CTGCATCTCG ACATCAAACC AGAAAACATC 
ATCGTCACTT ACATGAATGT GGTGAAGATT ATCGATTTTG GTAGTGCACA GACCTTCAAT 
CCTCTGTTCC TGAAGCAGTT CTCCCCACCT ATTGGAACAC TGGATTATAT GTCTCCAGAG 
ATGCTGAAAG GAGATGTGGT GGGTCCACCA GCTGACATCT GGAGCATTGG CATATTGACT 
TATATCATGC TTAGCGGGAG GTTGCCGTTC ACAGAAAATG ACCCTGCCGA GACCGAGGCC 
AGAATACAGG CAGCCAAGTT TGACCTCAGT AAGCTTTACC AAAACGTGTC CCAAAGTGCC 
TCTCTGTTCA TCAAAAAGAT CCTGTGCAGC TACCCTTGGG CCCGTCCGAC CATCAAGGAC 
TGCTTTACCA ACTCGTGGCT GCAGGACGCA TATCTGATGC GCCTGCGAAG ACAGACTCTC 
ACATTCACCA CCACACGCCT CAAAGAGTTC CTGGAGCAGC AGCAGCACAT CAGAGCCCAA 
GAGGCAACAA AGCACAAAGT CCTCCTGCGT TCCTACCAGA GCAACCCACA AACCCCATCC 
ACCCCTCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	spegb
Entrez Gene ID	563174
Full Name	SPEG complex locus b
Synonyms	si:ch211-195m20.1
General protein information	Preferred Names SPEG complex locus b Names SPEG complex locus b striated muscle preferentially expressed protein kinase
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Bos taurus (cattle)	SPEG	NP_001135957.1
Mus musculus (house mouse)	Speg	NP_031489.4
Rattus norvegicus (Norway rat)	Speg	NP_001102272.1
Gallus gallus (chicken)	SPEG	XP_003641690.2
Danio rerio (zebrafish)	si:ch211-195m20.1	XP_691634.6
Homo sapiens (human)	SPEG	NP_005867.3
Pan troglodytes (chimpanzee)	SPEG	NP_001166952.1
Macaca mulatta (Rhesus monkey)	SPEG	XP_001104264.2
Canis lupus familiaris (dog)	SPEG	NP_001166949.1
Xenopus tropicalis (tropical clawed frog)	speg	XP_002934009.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

spegb cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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