pfas cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	pfas
Entrez Gene ID	100216263
Full Name	phosphoribosylformylglycinamidine synthase
General protein information	Preferred Names phosphoribosylformylglycinamidine synthase Names phosphoribosylformylglycinamidine synthase
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001142220.3	NP_001135692.3	phosphoribosylformylglycinamidine synthase
XM_012970190.2	XP_012825644.1	phosphoribosylformylglycinamidine synthase isoform X1
XM_012970192.2	XP_012825646.1	phosphoribosylformylglycinamidine synthase isoform X2
XM_012970192.3	XP_012825646.2	phosphoribosylformylglycinamidine synthase isoform X2
XM_012970190.3	XP_012825644.2	phosphoribosylformylglycinamidine synthase isoform X1
NM_001142220.2	NP_001135692.2	phosphoribosylformylglycinamidine synthase
XM_012970191.2	XP_012825645.1	phosphoribosylformylglycinamidine synthase isoform X1

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	PFAS	XP_001112405.1
Pan troglodytes (chimpanzee)	PFAS	XP_511854.3
Bos taurus (cattle)	PFAS	NP_001243493.1
Mus musculus (house mouse)	Pfas	NP_001152991.1
Drosophila melanogaster (fruit fly)	ade2	NP_723146.1
Caenorhabditis elegans (roundworm)	CELE_F10F2.2	NP_497942.2
Homo sapiens (human)	PFAS	NP_036525.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002091	XP_320948.2
Canis lupus familiaris (dog)	PFAS	XP_005620078.1
Rattus norvegicus (Norway rat)	Pfas	NP_001099261.1
Arabidopsis thaliana (thale cress)	PUR4	NP_177566.3
Danio rerio (zebrafish)	pfas	NP_001038667.1
Oryza sativa (rice)	Os01g0888500	NP_001045039.1
Xenopus tropicalis (tropical clawed frog)	pfas	NP_001135692.2

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following pfas gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pfas cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa02847	NM_001142220.2 Latest version!	Xenopus (Silurana) tropicalis phosphoribosylformylglycinamidine synthase (pfas), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa32769	NM_001142220.3 Latest version!	Xenopus tropicalis phosphoribosylformylglycinamidine synthase (pfas), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa32769	XM_012970191.2 Latest version!	Xenopus tropicalis phosphoribosylformylglycinamidine synthase (pfas), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa32769	XM_012970190.2 Latest version!	Xenopus tropicalis phosphoribosylformylglycinamidine synthase (pfas), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa32770	XM_012970192.2 Latest version!	Xenopus tropicalis phosphoribosylformylglycinamidine synthase (pfas), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa65930	XM_012970190.3 Latest version!	Xenopus tropicalis phosphoribosylformylglycinamidine synthase (pfas), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa65931	XM_012970192.3 Latest version!	Xenopus tropicalis phosphoribosylformylglycinamidine synthase (pfas), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pfas ( NM_001142220.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001135692.2 Xenopus (Silurana) tropicalis phosphoribosylformylglycinamidine synthase (pfas), mRNA.

Price & Availability↑

CloneID	OXa02847
Clone ID Related Accession (Same CDS sequence)	NM_001142220.2
Accession Version	NM_001142220.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3975bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1366128000000
Organism	Xenopus tropicalis(Tropical clawed frog)
Product	phosphoribosylformylglycinamidine synthase
Comment	VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAMC01096052.1. On Sep 25, 2010 this sequence version replaced NM_001142220.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC168641.1 [ECO:0000332] ##Evidence-Data-END##

ATGGTGGTCC TGCACTTCTA TCGCTCCTTG CAGAATTCAT CAGAGACCCT GCGGCTGCCC 
GGTGCGGAGA ATATACAGAG CCTGCAGAGG GAGCTGTGCT ACAATGTCAA TTGGACAGGT 
CCCGCGGCCC CATCCAGCCA GGAGACTGAC GCCCTTCGAT GGCTGTTTAG TTGCCCCTTT 
GACCCTCAGA GTATCTCTGA CACCTCATCC CTTCACTCGG AGCCTTCTGA CCTGCTGGTA 
GAGATCGGCC CAAGACTGAA TTTCTCCACC GCGACTTCCA CCAATGCTGT GTCCATCTGC 
CGCTCTGTTG GCCTGACCAA CGTGGACAGG ATCGAGTGCT CCAAGAGGTA TCTCATCAAG 
TTCCAGACTA GGCCCCAGGG CAGTGAGGAG CAAGAGCTGC TAGCCTCTCT GTACGACCGT 
ATGACGGAAT GCGTTTACCC GGAGCCGGTG AGGAGTTTCG AGGTGTCCGT TAGACCGGAG 
AAAGTGTACG AGGTGGATAT TTTGGGGCAA GGCAGGGCAG CACTGGAAAA GGCCAACACT 
GAGCTAGGTT TGGCCTTCGA CTCCTGGGAT CTTGATTACT ATAGCAGCCT TTTCCAGCGC 
GTGGGTCGGA ACCCCAGCAG CGTGGAGTGC TTTGATCTTG CACAGTCCAA CAGCGAGCAC 
AGCCGCCACT GGTTCTTTAA GGGCAAATTA AAGGTCGACG GGCAGGAGAA GGAGATGTCT 
CTGTTCGACA TGATCATGAA GACTCAGGAC ACCAGTAATC CCAACAACGT CATCAAGTTC 
TGTGACAACA GCAGTGCGAT CCAGGGAAGA GAAGTGGCAT CTCTAATCCC AACAGACCCT 
TCATGTGCTG GGCCCTATCA TCTCTGTCGC TCCACACGGC ACCTGATTTT CACTGCAGAG 
ACCCACAACT TCCCTACTGG CGTGGCCCCA TTCAGCGGCG CTACGACAGG AACAGGAGGG 
CGCATTCGGG ACGTGCAGAG CACCGGCAGG GGAGCACATG TCATTGCTGG GACGGCTGGG 
TACTGCTTTG GGAACCTGCA CATCCCAGGA TACTCTCTTC CCTGGGAGGA CCCCGCATAT 
CAGTACCCAG TGCAGTTTGC CAGGCCTCTG GAGGTGGCTA TTGAAGCCAG TAACGGAGCC 
TCTGATTATG GAAACAAGTT TGGGGAGCCT GTTTTGGCCG GATTTGCCCG TTCCTTTGGA 
GTGCGGTTGC CTTCGGGAGA AAGGAGGGAG TGGGTGAAAC CCATTATGTT CAGTGGTGGG 
ATCGGATCCA TGGAGGATGT TCACCGTACT AAGGAGGCCC CAGAGCCTGG CATGCACGTG 
GTTAAAGTGG GAGGTCCTGT CTATAGGATT GGGGTTGGCG GTGGAGCTGC CTCTTCTATT 
CAGGTCCAGG GGGATAACGC CAGCGAGCTG GATTTTGGGG CAGTGCAACG TGGAGATGCA 
GAGATGGAGC AGAAGATGAA TCGGGCCGTA CGGGCCTGCG TGGAGCGAGG AGGCAAGAAC 
CCCATCTGCA GCATTCACGA TCAGGGAGCC GGGGGCAACG GGAACGTCCT GAAGGAGCTC 
AGTGAGCCCC AAGGAGCAGT CATTTACACT AAAAGCTTCC AGCTGGGGGA CCCCACCCTG 
AGTGTTCTGG AGATATGGGG AGCAGAATAT CAGGAGTCCA ACGCTTTGCT CCTGCGCCCC 
GACGATGCCG AATTCCTGCG CTCTGTGTGC CGGCGGGAGA GGTCTCCTGT GGATTTTGTG 
GGCAAGATCA CCGGGGATGG CAGGATTGTG TTGATAAATG GCAGTGACAC AGATCCCGCC 
CCTGACTCCA CTGACCGCAA TGCTGTTCCC GTTGACCTCC AGCTGGAATG GGTCTTAGGA 
AAAATGCCTC GCAAGGAGTT TGTTCTAAAC CGCGTGGCAC CAAACTTGCA GCCGCTGGCG 
CTCCCTAGTG GCCTGACAGT CGGGCAGGCG CTTGATCGCG TGCTGAGGTT GCCGTCCGTA 
GCGAGCAAAC GCTACCTGAC CAACAAGGTG GACCGCTCCG TCACAGGCCT GGTGGCCCAG 
CAGCAGTGCG TGGGGCCTCT TCACACTCCA TTGGCCGACG TGGCTGTGGT GTCTCTGTCT 
TATTCGGACA TTGTAGGAGG AGCTACAGCA ATAGGAGAAC AGCCAATCAA AAGCCTACTG 
AATCCTGCAG CTGGGGCAAG ACTGGCGGTG GCCGAGGCAC TAACCAATCT CCTCTTTGCT 
CAAGTGACGG ACCTAAAGGA TGTGAAATGC AGCGGGAACT GGATGTGGGC CGCCAAGCTG 
CCCGGAGAAG GCGCCTTGCT TTACGACGCC TGTGCAGCTA TGTGTGATGT CATGGCGCAG 
CTCGGCGTTG CCATCGATGG AGGAAAAGAT TCTCTCAGCA TGGCCGCCAG AGTGGGCACA 
GAAACAGTTA AAGCTCCAGG TTCGCTAGTG ATCTCGGTGT ATGCAGTCTG CCCCGACATA 
ACTACCACCG TGACCCCCGA TCTTAAGAAC CCAGGGCAGA AAGGTGTTTT ACTGTATTTG 
CCCCTCTGCC CTGGTCAGCA CCGGCTTGGG GGCAGCGCCC TGGCTCAGTG CTACTCACAG 
CTGGGAGAGA CCCCCCCAGA CCTCGATGAC CCTCAGACTT TAATCTCCTG CTTCAACGTC 
ACCCAACAGC TGCTGAGAGA CCGCGTCCTT AGCGCCGGCC ATGACGTCAG TGATGGAGGC 
CTAGTCACCT GTATCCTGGA AATGGCTTTT GCTGGAAACT GTGGCCTGGA TATTGAGATT 
TCCTCCTCCT GCACCAATGT GCTGGAACTG CTGTTTGCCG AGGAACCGGG CCTGGTTCTA 
GAGGTTGCAG AACAAAGTGT AGAACTGGTC ATGGAACGTT ACAGAGCAGC AGGAGTGGAA 
TGCGTGAGGA TCGGATGCAC CAAGGAACGT GGCCCAGCGT CTATGGTCCG AATCCGTGCC 
AATGGGCAGG AGGTGGTCAA CGAGAAGTTG GGTTCCCTCA GAGCCGTATG GGAGGAGACC 
AGTTTCCAAC TTGAACGCCT TCAGGCCAAT CCAAGCTGTG TATCGCAGGA GGAGGCTGGG 
CTGAGAGTGA GGGAGGGGCC AAGTTATCAC CTGACTTTTA ACCCGTCTGA AATACCTTTG 
GTTCCTCCAT CAGTCGGTGG CAACCAACCG CGTGTTGCTG TTGTGCGTGA AGAGGGTAGC 
AACGGAGACC GGGAAATGGC GGCATCTCTC CTAATGGCTG GATTTCAGGT GTGGGATGTC 
ACCATGGAAG ATCTTCTTGC TGGAGGCACC ACCCTGGACT CTTTCAGGGG ACTTGTATTT 
GTTGGGGGAT TTAGCTACGC TGATGTGTTA GGGTCTGCCA AAGGTTGGGC CGCCTCGGTG 
AAGTTCAATG CCAGCGTGCG CGAGCAGTTC GAGAACTTCC GCCGTAGGGC AGATACTTTC 
AGCCTTGGGG TTTGCAATGG GTGCCAGCTG ATGGCTCTTC TGGGCTGGGT AGGACCCGAT 
AACCCAACAG ACACAGTAGG TGATCTCCCC ACCCAAGGGG TTCTGCTCAG CCACAATCTC 
TCCGGCCGGT TTGAATCTCG ATTTGTCACC CTGAAGATCC AACAGAGTCC TTCCATTTTG 
CTACGAGGGA TGGCCGGGTC CACGCTCGGA GTGTGGGTGG CCCATGGCGA AGGATACATG 
AGGTTCCGAT CTCCGAAAGT GCAGGACTAT GTGACGTCCA ATCACTTGGC GCCGCTCTGT 
TACGTGGATG ATGGAGGCAA GCCGACGGAA GAATACCCAA TGAATCCAAA TGGCTCTCCG 
TTGGGAATTG CTGGTCTTTG CTCTGCAGAT GGGCGGCACT TGGCCATGAT GCCCCACCCA 
GAGCGCTGTG TCATGAAGTG GCAGTGGCCT TGGATGCCGG AAAGCTGGCG CCACTCATTG 
GATGTCTCGC CGTGGATGCG TCTCTTCCAG AATGGGTACC GCTGGTGCCA GGAGAACCCA 
AGCAGGGATA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001135692.2
CDS	46..4020
Translation	MVVLHFYRSLQNSSETLRLPGAENIQSLQRELCYNVNWTGPAAPSSQETDALRWLFSCPFDPQSISDTSSLHSEPSDLLVEIGPRLNFSTATSTNAVSICRSVGLTNVDRIECSKRYLIKFQTRPQGSEEQELLASLYDRMTECVYPEPVRSFEVSVRPEKVYEVDILGQGRAALEKANTELGLAFDSWDLDYYSSLFQRVGRNPSSVECFDLAQSNSEHSRHWFFKGKLKVDGQEKEMSLFDMIMKTQDTSNPNNVIKFCDNSSAIQGREVASLIPTDPSCAGPYHLCRSTRHLIFTAETHNFPTGVAPFSGATTGTGGRIRDVQSTGRGAHVIAGTAGYCFGNLHIPGYSLPWEDPAYQYPVQFARPLEVAIEASNGASDYGNKFGEPVLAGFARSFGVRLPSGERREWVKPIMFSGGIGSMEDVHRTKEAPEPGMHVVKVGGPVYRIGVGGGAASSIQVQGDNASELDFGAVQRGDAEMEQKMNRAVRACVERGGKNPICSIHDQGAGGNGNVLKELSEPQGAVIYTKSFQLGDPTLSVLEIWGAEYQESNALLLRPDDAEFLRSVCRRERSPVDFVGKITGDGRIVLINGSDTDPAPDSTDRNAVPVDLQLEWVLGKMPRKEFVLNRVAPNLQPLALPSGLTVGQALDRVLRLPSVASKRYLTNKVDRSVTGLVAQQQCVGPLHTPLADVAVVSLSYSDIVGGATAIGEQPIKSLLNPAAGARLAVAEALTNLLFAQVTDLKDVKCSGNWMWAAKLPGEGALLYDACAAMCDVMAQLGVAIDGGKDSLSMAARVGTETVKAPGSLVISVYAVCPDITTTVTPDLKNPGQKGVLLYLPLCPGQHRLGGSALAQCYSQLGETPPDLDDPQTLISCFNVTQQLLRDRVLSAGHDVSDGGLVTCILEMAFAGNCGLDIEISSSCTNVLELLFAEEPGLVLEVAEQSVELVMERYRAAGVECVRIGCTKERGPASMVRIRANGQEVVNEKLGSLRAVWEETSFQLERLQANPSCVSQEEAGLRVREGPSYHLTFNPSEIPLVPPSVGGNQPRVAVVREEGSNGDREMAASLLMAGFQVWDVTMEDLLAGGTTLDSFRGLVFVGGFSYADVLGSAKGWAASVKFNASVREQFENFRRRADTFSLGVCNGCQLMALLGWVGPDNPTDTVGDLPTQGVLLSHNLSGRFESRFVTLKIQQSPSILLRGMAGSTLGVWVAHGEGYMRFRSPKVQDYVTSNHLAPLCYVDDGGKPTEEYPMNPNGSPLGIAGLCSADGRHLAMMPHPERCVMKWQWPWMPESWRHSLDVSPWMRLFQNGYRWCQENPSRDN

Target ORF information:

RefSeq Version	NM_001142220.2
Organism	Xenopus tropicalis(Tropical clawed frog)
Definition	Xenopus (Silurana) tropicalis phosphoribosylformylglycinamidine synthase (pfas), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142220.2

ORF Insert Sequence:

ATGGTGGTCC TGCACTTCTA TCGCTCCTTG CAGAATTCAT CAGAGACCCT GCGGCTGCCC 
GGTGCGGAGA ATATACAGAG CCTGCAGAGG GAGCTGTGCT ACAATGTCAA TTGGACAGGT 
CCCGCGGCCC CATCCAGCCA GGAGACTGAC GCCCTTCGAT GGCTGTTTAG TTGCCCCTTT 
GACCCTCAGA GTATCTCTGA CACCTCATCC CTTCACTCGG AGCCTTCTGA CCTGCTGGTA 
GAGATCGGCC CAAGACTGAA TTTCTCCACC GCGACTTCCA CCAATGCTGT GTCCATCTGC 
CGCTCTGTTG GCCTGACCAA CGTGGACAGG ATCGAGTGCT CCAAGAGGTA TCTCATCAAG 
TTCCAGACTA GGCCCCAGGG CAGTGAGGAG CAAGAGCTGC TAGCCTCTCT GTACGACCGT 
ATGACGGAAT GCGTTTACCC GGAGCCGGTG AGGAGTTTCG AGGTGTCCGT TAGACCGGAG 
AAAGTGTACG AGGTGGATAT TTTGGGGCAA GGCAGGGCAG CACTGGAAAA GGCCAACACT 
GAGCTAGGTT TGGCCTTCGA CTCCTGGGAT CTTGATTACT ATAGCAGCCT TTTCCAGCGC 
GTGGGTCGGA ACCCCAGCAG CGTGGAGTGC TTTGATCTTG CACAGTCCAA CAGCGAGCAC 
AGCCGCCACT GGTTCTTTAA GGGCAAATTA AAGGTCGACG GGCAGGAGAA GGAGATGTCT 
CTGTTCGACA TGATCATGAA GACTCAGGAC ACCAGTAATC CCAACAACGT CATCAAGTTC 
TGTGACAACA GCAGTGCGAT CCAGGGAAGA GAAGTGGCAT CTCTAATCCC AACAGACCCT 
TCATGTGCTG GGCCCTATCA TCTCTGTCGC TCCACACGGC ACCTGATTTT CACTGCAGAG 
ACCCACAACT TCCCTACTGG CGTGGCCCCA TTCAGCGGCG CTACGACAGG AACAGGAGGG 
CGCATTCGGG ACGTGCAGAG CACCGGCAGG GGAGCACATG TCATTGCTGG GACGGCTGGG 
TACTGCTTTG GGAACCTGCA CATCCCAGGA TACTCTCTTC CCTGGGAGGA CCCCGCATAT 
CAGTACCCAG TGCAGTTTGC CAGGCCTCTG GAGGTGGCTA TTGAAGCCAG TAACGGAGCC 
TCTGATTATG GAAACAAGTT TGGGGAGCCT GTTTTGGCCG GATTTGCCCG TTCCTTTGGA 
GTGCGGTTGC CTTCGGGAGA AAGGAGGGAG TGGGTGAAAC CCATTATGTT CAGTGGTGGG 
ATCGGATCCA TGGAGGATGT TCACCGTACT AAGGAGGCCC CAGAGCCTGG CATGCACGTG 
GTTAAAGTGG GAGGTCCTGT CTATAGGATT GGGGTTGGCG GTGGAGCTGC CTCTTCTATT 
CAGGTCCAGG GGGATAACGC CAGCGAGCTG GATTTTGGGG CAGTGCAACG TGGAGATGCA 
GAGATGGAGC AGAAGATGAA TCGGGCCGTA CGGGCCTGCG TGGAGCGAGG AGGCAAGAAC 
CCCATCTGCA GCATTCACGA TCAGGGAGCC GGGGGCAACG GGAACGTCCT GAAGGAGCTC 
AGTGAGCCCC AAGGAGCAGT CATTTACACT AAAAGCTTCC AGCTGGGGGA CCCCACCCTG 
AGTGTTCTGG AGATATGGGG AGCAGAATAT CAGGAGTCCA ACGCTTTGCT CCTGCGCCCC 
GACGATGCCG AATTCCTGCG CTCTGTGTGC CGGCGGGAGA GGTCTCCTGT GGATTTTGTG 
GGCAAGATCA CCGGGGATGG CAGGATTGTG TTGATAAATG GCAGTGACAC AGATCCCGCC 
CCTGACTCCA CTGACCGCAA TGCTGTTCCC GTTGACCTCC AGCTGGAATG GGTCTTAGGA 
AAAATGCCTC GCAAGGAGTT TGTTCTAAAC CGCGTGGCAC CAAACTTGCA GCCGCTGGCG 
CTCCCTAGTG GCCTGACAGT CGGGCAGGCG CTTGATCGCG TGCTGAGGTT GCCGTCCGTA 
GCGAGCAAAC GCTACCTGAC CAACAAGGTG GACCGCTCCG TCACAGGCCT GGTGGCCCAG 
CAGCAGTGCG TGGGGCCTCT TCACACTCCA TTGGCCGACG TGGCTGTGGT GTCTCTGTCT 
TATTCGGACA TTGTAGGAGG AGCTACAGCA ATAGGAGAAC AGCCAATCAA AAGCCTACTG 
AATCCTGCAG CTGGGGCAAG ACTGGCGGTG GCCGAGGCAC TAACCAATCT CCTCTTTGCT 
CAAGTGACGG ACCTAAAGGA TGTGAAATGC AGCGGGAACT GGATGTGGGC CGCCAAGCTG 
CCCGGAGAAG GCGCCTTGCT TTACGACGCC TGTGCAGCTA TGTGTGATGT CATGGCGCAG 
CTCGGCGTTG CCATCGATGG AGGAAAAGAT TCTCTCAGCA TGGCCGCCAG AGTGGGCACA 
GAAACAGTTA AAGCTCCAGG TTCGCTAGTG ATCTCGGTGT ATGCAGTCTG CCCCGACATA 
ACTACCACCG TGACCCCCGA TCTTAAGAAC CCAGGGCAGA AAGGTGTTTT ACTGTATTTG 
CCCCTCTGCC CTGGTCAGCA CCGGCTTGGG GGCAGCGCCC TGGCTCAGTG CTACTCACAG 
CTGGGAGAGA CCCCCCCAGA CCTCGATGAC CCTCAGACTT TAATCTCCTG CTTCAACGTC 
ACCCAACAGC TGCTGAGAGA CCGCGTCCTT AGCGCCGGCC ATGACGTCAG TGATGGAGGC 
CTAGTCACCT GTATCCTGGA AATGGCTTTT GCTGGAAACT GTGGCCTGGA TATTGAGATT 
TCCTCCTCCT GCACCAATGT GCTGGAACTG CTGTTTGCCG AGGAACCGGG CCTGGTTCTA 
GAGGTTGCAG AACAAAGTGT AGAACTGGTC ATGGAACGTT ACAGAGCAGC AGGAGTGGAA 
TGCGTGAGGA TCGGATGCAC CAAGGAACGT GGCCCAGCGT CTATGGTCCG AATCCGTGCC 
AATGGGCAGG AGGTGGTCAA CGAGAAGTTG GGTTCCCTCA GAGCCGTATG GGAGGAGACC 
AGTTTCCAAC TTGAACGCCT TCAGGCCAAT CCAAGCTGTG TATCGCAGGA GGAGGCTGGG 
CTGAGAGTGA GGGAGGGGCC AAGTTATCAC CTGACTTTTA ACCCGTCTGA AATACCTTTG 
GTTCCTCCAT CAGTCGGTGG CAACCAACCG CGTGTTGCTG TTGTGCGTGA AGAGGGTAGC 
AACGGAGACC GGGAAATGGC GGCATCTCTC CTAATGGCTG GATTTCAGGT GTGGGATGTC 
ACCATGGAAG ATCTTCTTGC TGGAGGCACC ACCCTGGACT CTTTCAGGGG ACTTGTATTT 
GTTGGGGGAT TTAGCTACGC TGATGTGTTA GGGTCTGCCA AAGGTTGGGC CGCCTCGGTG 
AAGTTCAATG CCAGCGTGCG CGAGCAGTTC GAGAACTTCC GCCGTAGGGC AGATACTTTC 
AGCCTTGGGG TTTGCAATGG GTGCCAGCTG ATGGCTCTTC TGGGCTGGGT AGGACCCGAT 
AACCCAACAG ACACAGTAGG TGATCTCCCC ACCCAAGGGG TTCTGCTCAG CCACAATCTC 
TCCGGCCGGT TTGAATCTCG ATTTGTCACC CTGAAGATCC AACAGAGTCC TTCCATTTTG 
CTACGAGGGA TGGCCGGGTC CACGCTCGGA GTGTGGGTGG CCCATGGCGA AGGATACATG 
AGGTTCCGAT CTCCGAAAGT GCAGGACTAT GTGACGTCCA ATCACTTGGC GCCGCTCTGT 
TACGTGGATG ATGGAGGCAA GCCGACGGAA GAATACCCAA TGAATCCAAA TGGCTCTCCG 
TTGGGAATTG CTGGTCTTTG CTCTGCAGAT GGGCGGCACT TGGCCATGAT GCCCCACCCA 
GAGCGCTGTG TCATGAAGTG GCAGTGGCCT TGGATGCCGG AAAGCTGGCG CCACTCATTG 
GATGTCTCGC CGTGGATGCG TCTCTTCCAG AATGGGTACC GCTGGTGCCA GGAGAACCCA 
AGCAGGGATA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pfas ( NM_001142220.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001135692.3 Xenopus tropicalis phosphoribosylformylglycinamidine synthase (pfas), mRNA.

Price & Availability↑

CloneID	OXa32769
Clone ID Related Accession (Same CDS sequence)	NM_001142220.3 , XM_012970190.2 , XM_012970191.2
Accession Version	NM_001142220.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3975bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1499270400000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	phosphoribosylformylglycinamidine synthase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAMC03033817.1. On Jul 7, 2017 this sequence version replaced NM_001142220.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC168641.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1025683, SAMEA1025684 [ECO:0000350] ##Evidence-Data-END##

ATGGTGGTCC TGCACTTCTA TCGCTCCTTG CAGAATTCAT CAGAGACCCT GCGGCTGCCC 
GGTGCGGAGA ATATACAGAG CCTGCAGAGG GAGCTGTGCT ACAATGTCAA TTGGACAGGT 
CCCGCGGCCC CATCCAGCCA GGAGACTGAC GCCCTTCGAT GGCTGTTTAG TTGCCCCTTT 
GACCCTCAGA GTATCTCTGA CACCTCATCC CTTCACTCGG AGCCTTCTGA CCTGCTGGTA 
GAGATCGGCC CAAGACTGAA TTTCTCCACC GCGACTTCCA CCAATGCTGT GTCCATCTGC 
CGCTCTGTTG GCCTGACCAA CGTGGACAGG ATCGAGTGCT CCAAGAGGTA TCTCATCAAG 
TTCCAGACTA GGCCCCAGGG CAGTGAGGAG CAAGAGCTGC TAGCCTCTCT GTACGACCGT 
ATGACGGAAT GCGTTTACCC GGAGCCGGTG AGGAGTTTCG AGGTGTCCGT TAGACCGGAG 
AAAGTGTACG AGGTGGATAT TTTGGGGCAA GGCAGGGCAG CACTGGAAAA GGCCAACACT 
GAGCTAGGTT TGGCCTTCGA CTCCTGGGAT CTTGATTACT ATAGCAGCCT TTTCCAGCGC 
GTGGGTCGGA ACCCCAGCAG CGTGGAGTGC TTTGATCTTG CACAGTCCAA CAGCGAGCAC 
AGCCGCCACT GGTTCTTTAA GGGCAAATTA AAGGTCGACG GGCAGGAGAA GGAGATGTCT 
CTGTTCGACA TGATCATGAA GACTCAGGAC ACCAGTAATC CCAACAACGT CATCAAGTTC 
TGTGACAACA GCAGTGCGAT CCAGGGAAGA GAAGTGGCAT CTCTAATCCC AACAGACCCT 
TCATGTGCTG GGCCCTATCA TCTCTGTCGC TCCACACGGC ACCTGATTTT CACTGCAGAG 
ACCCACAACT TCCCTACTGG CGTGGCCCCA TTCAGCGGCG CTACGACAGG AACAGGAGGG 
CGCATTCGGG ACGTGCAGAG CACCGGCAGG GGAGCACATG TCATTGCTGG GACGGCTGGG 
TACTGCTTTG GGAACCTGCA CATCCCAGGA TACTCTCTTC CCTGGGAGGA CCTCGCATAT 
CAGTACCCAG TGCAGTTTGC CAGGCCTCTG GAGGTGGCTA TTGAAGCCAG TAACGGAGCC 
TCTGATTATG GAAACAAGTT TGGGGAGCCT GTTTTGGCCG GATTTGCCCG TTCCTTTGGC 
CTGCGGTTGC CTTCGGGAGA AAGGAGGGAG TGGGTGAAAC CCATTATGTT CAGTGGTGGG 
ATCGGATCCA TGGAGGATGT TCACCGTACT AAGGAGGCCC CAGAGCCTGG CATGCACGTG 
GTTAAAGTGG GAGGTCCTGT CTATAGGATT GGGGTTGGCG GTGGAGCTGC CTCTTCTATT 
CAGGTCCAGG GGGATAACGC CAGCGAGCTG GATTTTGGGG CAGTGCAACG TGGAGATGCA 
GAGATGGAGC AGAAGATGAA TCGGGCCGTA CGGGCCTGCG TGGAGCGAGG AGGCAAGAAC 
CCCATCTGCA GCATTCACGA TCAGGGAGCC GGGGGCAACG GGAACGTCCT GAAGGAGCTC 
AGTGAGCCCC AAGGAGCAGT CATTTACACT AAAAGCTTCC AGCTGGGGGA CCCCACCCTG 
AGTGTTCTGG AGATATGGGG AGCAGAATAT CAGGAGTCCA ACGCTTTGCT CCTGCGCCCC 
GACGATGCCG AATTCCTGCG CTCTGTGTGC CGGCGGGAGA GGTCTCCTGT GGATTTTGTG 
GGCAAGATCA CCGGGGATGG CAGGATTGTG TTGATAAATG GCAGTGACAC AGATCCCGCC 
CCTGACTCCA CTGACCGCAA TGCTGTTCCC GTTGACCTCC AGCTGGAATG GGTCTTAGGA 
AAAATGCCTC GCAAGGAGTT TGTTCTAAAC CGCGTGGCAC CAAACTTGCA GCCGCTGGTG 
CTCCCTAGTG GCCTGACAGT CGGGCAGGCG CTTGATCGCG TGCTGAGGTT GCCGTCCGTA 
GCGAGCAAAC GCTACCTGAC CAACAAGGTG GACCGCTCCG TCACAGGCCT GGTGGCCCAG 
CAGCAGTGCG TGGGGCCTCT TCACACTCCA TTGGCCGACG TGGCTGTGGT GTCTCTGTCT 
TATTCGGACA TTGTAGGAGG AGCTACAGCA ATAGGAGAAC AGCCAATCAA AAGCCTACTG 
AATCCTGCAG CTGGGGCAAG ACTGGCGGTG GCCGAGGCAC TAACCAATCT CCTCTTTGCT 
CAAGTGACGG ACCTAAAGGA TGTGAAATGC AGCGGGAACT GGATGTGGGC CGCCAAGCTG 
CCCGGAGAAG GCGCCTTGCT TTACGACGCC TGTGCAGCTA TGTGTGATGT CATGGCGCAG 
CTCGGCGTTG CCATCGATGG AGGAAAAGAT TCTCTCAGCA TGGCCGCCAG AGTGGGCACA 
GAAACAGTTA AAGCTCCAGG TTCGCTAGTG ATCTCGGTGT ATGCAGTCTG CCCCGACATA 
ACTACCACCG TGACCCCCGA TCTTAAGAAC CCAGGGCAGA AAGGTGTTTT ACTGTATTTG 
CCCCTCTGCC CTGGTCAGCA CCGGCTTGGG GGCAGCGCCC TGGCTCAGTG CTACTCACAG 
CTGGGAGAGA CCCCCCCAGA CCTCGATGAC CCTCAGACTT TAATCTCCTG CTTCAACGTC 
ACCCAACAGC TGCTGAGAGA CCGCGTCCTT AGCGCCGGCC ATGACGTCAG TGATGGAGGC 
CTAGTCACGT GTATCCTGGA AATGGCTTTT GCTGGAAACT GTGGCCTGGA TATTGAGATT 
TCCTCCTCCT GCACCAATGT GCTGGAACTG CTGTTTGCCG AGGAACCGGG CCTGGTTCTA 
GAGGTTGCAG AACAAAGTGT AGAACTGGTC ATGGAACGTT ACAGAGCAGC AGGAGTGGAA 
TGCGTGAGGA TCGGATGCAC CAAGGAACGT GGCCCAGCGT CTATGGTCCG AATCCGTGCC 
AATGGGCAGG AGGTGGTCAA CGAGAAGTTG GGTTCCCTCA GAGCCGTATG GGAGGAGACC 
AGTTTCCAAC TTGAACGCCT TCAGGCCAAT CCAAGCTGTG TATCGCAGGA GGAGGCTGGG 
CTGAGAGTGA GGGAGGGGCC AAGTTATCAC CTGACTTTTA ACCCGTCTGA AATACCTTTG 
GTTCCTCCAT CAGTCGGTGG CAACCAACCG CGTGTTGCTG TTGTGCGTGA AGAGGGTAGC 
AACGGAGACC GGGAAATGGC GGCATCTCTC CTAATGGCTG GATTTCAGGT GTGGGATGTC 
ACCATGGAAG ATCTTCTTGC TGGAGGCACC ACCCTGGACT CTTTCAGGGG ACTTGTATTT 
GTTGGGGGAT TTAGCTACGC TGATGTGTTA GGGTCTGCCA AAGGTTGGGC CGCCTCGGTG 
AAGTTCAATG CCAGCGTGCG CGAGCAGTTC GAGAACTTCC GCCGTAGGGC AGATACTTTC 
AGCCTTGGGG TTTGCAATGG GTGCCAGCTG ATGGCTCTTC TGGGCTGGGT AGGACCCGAT 
AACCCAACAG ACACAGTAGG TGATCTCCCC ACCCAAGGGG TTCTGCTCAG CCACAATCTC 
TCCGGCCGGT TTGAATCTCG ATTTGTCACC CTGAAGATCC AACAGAGTCC TTCCATTTTG 
CTACGAGGGA TGGCCGGGTC CACGCTCGGA GTGTGGGTGG CCCATGGCGA AGGATACATG 
AGGTTCCGAT CTCCGAAAGT GCAGGACTAT GTGACGTCCA ATCACTTGGC GCCGCTCTGT 
TACGTGGATG ATGGAGGCAA GCCGACGGAA GAATACCCAA TGAATCCAAA TGGCTCTCCG 
TTGGGAATTG CTGGTCTTTG CTCTGCAGAT GGGCGGCACT TGGCCATGAT GCCCCACCCA 
GAGCGCTGTG TCATGAAGTG GCAGTGGCCT TGGATGCCGG AAAGCTGGCG CCACTCATTG 
GATGTCTCGC CGTGGATGCG TCTCTTCCAG AATGGGTACC GCTGGTGCCA GGAGAACCCA 
AGCAGGGATA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001135692.3
CDS	46..4020
Translation	MVVLHFYRSLQNSSETLRLPGAENIQSLQRELCYNVNWTGPAAPSSQETDALRWLFSCPFDPQSISDTSSLHSEPSDLLVEIGPRLNFSTATSTNAVSICRSVGLTNVDRIECSKRYLIKFQTRPQGSEEQELLASLYDRMTECVYPEPVRSFEVSVRPEKVYEVDILGQGRAALEKANTELGLAFDSWDLDYYSSLFQRVGRNPSSVECFDLAQSNSEHSRHWFFKGKLKVDGQEKEMSLFDMIMKTQDTSNPNNVIKFCDNSSAIQGREVASLIPTDPSCAGPYHLCRSTRHLIFTAETHNFPTGVAPFSGATTGTGGRIRDVQSTGRGAHVIAGTAGYCFGNLHIPGYSLPWEDLAYQYPVQFARPLEVAIEASNGASDYGNKFGEPVLAGFARSFGLRLPSGERREWVKPIMFSGGIGSMEDVHRTKEAPEPGMHVVKVGGPVYRIGVGGGAASSIQVQGDNASELDFGAVQRGDAEMEQKMNRAVRACVERGGKNPICSIHDQGAGGNGNVLKELSEPQGAVIYTKSFQLGDPTLSVLEIWGAEYQESNALLLRPDDAEFLRSVCRRERSPVDFVGKITGDGRIVLINGSDTDPAPDSTDRNAVPVDLQLEWVLGKMPRKEFVLNRVAPNLQPLVLPSGLTVGQALDRVLRLPSVASKRYLTNKVDRSVTGLVAQQQCVGPLHTPLADVAVVSLSYSDIVGGATAIGEQPIKSLLNPAAGARLAVAEALTNLLFAQVTDLKDVKCSGNWMWAAKLPGEGALLYDACAAMCDVMAQLGVAIDGGKDSLSMAARVGTETVKAPGSLVISVYAVCPDITTTVTPDLKNPGQKGVLLYLPLCPGQHRLGGSALAQCYSQLGETPPDLDDPQTLISCFNVTQQLLRDRVLSAGHDVSDGGLVTCILEMAFAGNCGLDIEISSSCTNVLELLFAEEPGLVLEVAEQSVELVMERYRAAGVECVRIGCTKERGPASMVRIRANGQEVVNEKLGSLRAVWEETSFQLERLQANPSCVSQEEAGLRVREGPSYHLTFNPSEIPLVPPSVGGNQPRVAVVREEGSNGDREMAASLLMAGFQVWDVTMEDLLAGGTTLDSFRGLVFVGGFSYADVLGSAKGWAASVKFNASVREQFENFRRRADTFSLGVCNGCQLMALLGWVGPDNPTDTVGDLPTQGVLLSHNLSGRFESRFVTLKIQQSPSILLRGMAGSTLGVWVAHGEGYMRFRSPKVQDYVTSNHLAPLCYVDDGGKPTEEYPMNPNGSPLGIAGLCSADGRHLAMMPHPERCVMKWQWPWMPESWRHSLDVSPWMRLFQNGYRWCQENPSRDN

Target ORF information:

RefSeq Version	NM_001142220.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphoribosylformylglycinamidine synthase (pfas), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142220.3

ORF Insert Sequence:

ATGGTGGTCC TGCACTTCTA TCGCTCCTTG CAGAATTCAT CAGAGACCCT GCGGCTGCCC 
GGTGCGGAGA ATATACAGAG CCTGCAGAGG GAGCTGTGCT ACAATGTCAA TTGGACAGGT 
CCCGCGGCCC CATCCAGCCA GGAGACTGAC GCCCTTCGAT GGCTGTTTAG TTGCCCCTTT 
GACCCTCAGA GTATCTCTGA CACCTCATCC CTTCACTCGG AGCCTTCTGA CCTGCTGGTA 
GAGATCGGCC CAAGACTGAA TTTCTCCACC GCGACTTCCA CCAATGCTGT GTCCATCTGC 
CGCTCTGTTG GCCTGACCAA CGTGGACAGG ATCGAGTGCT CCAAGAGGTA TCTCATCAAG 
TTCCAGACTA GGCCCCAGGG CAGTGAGGAG CAAGAGCTGC TAGCCTCTCT GTACGACCGT 
ATGACGGAAT GCGTTTACCC GGAGCCGGTG AGGAGTTTCG AGGTGTCCGT TAGACCGGAG 
AAAGTGTACG AGGTGGATAT TTTGGGGCAA GGCAGGGCAG CACTGGAAAA GGCCAACACT 
GAGCTAGGTT TGGCCTTCGA CTCCTGGGAT CTTGATTACT ATAGCAGCCT TTTCCAGCGC 
GTGGGTCGGA ACCCCAGCAG CGTGGAGTGC TTTGATCTTG CACAGTCCAA CAGCGAGCAC 
AGCCGCCACT GGTTCTTTAA GGGCAAATTA AAGGTCGACG GGCAGGAGAA GGAGATGTCT 
CTGTTCGACA TGATCATGAA GACTCAGGAC ACCAGTAATC CCAACAACGT CATCAAGTTC 
TGTGACAACA GCAGTGCGAT CCAGGGAAGA GAAGTGGCAT CTCTAATCCC AACAGACCCT 
TCATGTGCTG GGCCCTATCA TCTCTGTCGC TCCACACGGC ACCTGATTTT CACTGCAGAG 
ACCCACAACT TCCCTACTGG CGTGGCCCCA TTCAGCGGCG CTACGACAGG AACAGGAGGG 
CGCATTCGGG ACGTGCAGAG CACCGGCAGG GGAGCACATG TCATTGCTGG GACGGCTGGG 
TACTGCTTTG GGAACCTGCA CATCCCAGGA TACTCTCTTC CCTGGGAGGA CCTCGCATAT 
CAGTACCCAG TGCAGTTTGC CAGGCCTCTG GAGGTGGCTA TTGAAGCCAG TAACGGAGCC 
TCTGATTATG GAAACAAGTT TGGGGAGCCT GTTTTGGCCG GATTTGCCCG TTCCTTTGGC 
CTGCGGTTGC CTTCGGGAGA AAGGAGGGAG TGGGTGAAAC CCATTATGTT CAGTGGTGGG 
ATCGGATCCA TGGAGGATGT TCACCGTACT AAGGAGGCCC CAGAGCCTGG CATGCACGTG 
GTTAAAGTGG GAGGTCCTGT CTATAGGATT GGGGTTGGCG GTGGAGCTGC CTCTTCTATT 
CAGGTCCAGG GGGATAACGC CAGCGAGCTG GATTTTGGGG CAGTGCAACG TGGAGATGCA 
GAGATGGAGC AGAAGATGAA TCGGGCCGTA CGGGCCTGCG TGGAGCGAGG AGGCAAGAAC 
CCCATCTGCA GCATTCACGA TCAGGGAGCC GGGGGCAACG GGAACGTCCT GAAGGAGCTC 
AGTGAGCCCC AAGGAGCAGT CATTTACACT AAAAGCTTCC AGCTGGGGGA CCCCACCCTG 
AGTGTTCTGG AGATATGGGG AGCAGAATAT CAGGAGTCCA ACGCTTTGCT CCTGCGCCCC 
GACGATGCCG AATTCCTGCG CTCTGTGTGC CGGCGGGAGA GGTCTCCTGT GGATTTTGTG 
GGCAAGATCA CCGGGGATGG CAGGATTGTG TTGATAAATG GCAGTGACAC AGATCCCGCC 
CCTGACTCCA CTGACCGCAA TGCTGTTCCC GTTGACCTCC AGCTGGAATG GGTCTTAGGA 
AAAATGCCTC GCAAGGAGTT TGTTCTAAAC CGCGTGGCAC CAAACTTGCA GCCGCTGGTG 
CTCCCTAGTG GCCTGACAGT CGGGCAGGCG CTTGATCGCG TGCTGAGGTT GCCGTCCGTA 
GCGAGCAAAC GCTACCTGAC CAACAAGGTG GACCGCTCCG TCACAGGCCT GGTGGCCCAG 
CAGCAGTGCG TGGGGCCTCT TCACACTCCA TTGGCCGACG TGGCTGTGGT GTCTCTGTCT 
TATTCGGACA TTGTAGGAGG AGCTACAGCA ATAGGAGAAC AGCCAATCAA AAGCCTACTG 
AATCCTGCAG CTGGGGCAAG ACTGGCGGTG GCCGAGGCAC TAACCAATCT CCTCTTTGCT 
CAAGTGACGG ACCTAAAGGA TGTGAAATGC AGCGGGAACT GGATGTGGGC CGCCAAGCTG 
CCCGGAGAAG GCGCCTTGCT TTACGACGCC TGTGCAGCTA TGTGTGATGT CATGGCGCAG 
CTCGGCGTTG CCATCGATGG AGGAAAAGAT TCTCTCAGCA TGGCCGCCAG AGTGGGCACA 
GAAACAGTTA AAGCTCCAGG TTCGCTAGTG ATCTCGGTGT ATGCAGTCTG CCCCGACATA 
ACTACCACCG TGACCCCCGA TCTTAAGAAC CCAGGGCAGA AAGGTGTTTT ACTGTATTTG 
CCCCTCTGCC CTGGTCAGCA CCGGCTTGGG GGCAGCGCCC TGGCTCAGTG CTACTCACAG 
CTGGGAGAGA CCCCCCCAGA CCTCGATGAC CCTCAGACTT TAATCTCCTG CTTCAACGTC 
ACCCAACAGC TGCTGAGAGA CCGCGTCCTT AGCGCCGGCC ATGACGTCAG TGATGGAGGC 
CTAGTCACGT GTATCCTGGA AATGGCTTTT GCTGGAAACT GTGGCCTGGA TATTGAGATT 
TCCTCCTCCT GCACCAATGT GCTGGAACTG CTGTTTGCCG AGGAACCGGG CCTGGTTCTA 
GAGGTTGCAG AACAAAGTGT AGAACTGGTC ATGGAACGTT ACAGAGCAGC AGGAGTGGAA 
TGCGTGAGGA TCGGATGCAC CAAGGAACGT GGCCCAGCGT CTATGGTCCG AATCCGTGCC 
AATGGGCAGG AGGTGGTCAA CGAGAAGTTG GGTTCCCTCA GAGCCGTATG GGAGGAGACC 
AGTTTCCAAC TTGAACGCCT TCAGGCCAAT CCAAGCTGTG TATCGCAGGA GGAGGCTGGG 
CTGAGAGTGA GGGAGGGGCC AAGTTATCAC CTGACTTTTA ACCCGTCTGA AATACCTTTG 
GTTCCTCCAT CAGTCGGTGG CAACCAACCG CGTGTTGCTG TTGTGCGTGA AGAGGGTAGC 
AACGGAGACC GGGAAATGGC GGCATCTCTC CTAATGGCTG GATTTCAGGT GTGGGATGTC 
ACCATGGAAG ATCTTCTTGC TGGAGGCACC ACCCTGGACT CTTTCAGGGG ACTTGTATTT 
GTTGGGGGAT TTAGCTACGC TGATGTGTTA GGGTCTGCCA AAGGTTGGGC CGCCTCGGTG 
AAGTTCAATG CCAGCGTGCG CGAGCAGTTC GAGAACTTCC GCCGTAGGGC AGATACTTTC 
AGCCTTGGGG TTTGCAATGG GTGCCAGCTG ATGGCTCTTC TGGGCTGGGT AGGACCCGAT 
AACCCAACAG ACACAGTAGG TGATCTCCCC ACCCAAGGGG TTCTGCTCAG CCACAATCTC 
TCCGGCCGGT TTGAATCTCG ATTTGTCACC CTGAAGATCC AACAGAGTCC TTCCATTTTG 
CTACGAGGGA TGGCCGGGTC CACGCTCGGA GTGTGGGTGG CCCATGGCGA AGGATACATG 
AGGTTCCGAT CTCCGAAAGT GCAGGACTAT GTGACGTCCA ATCACTTGGC GCCGCTCTGT 
TACGTGGATG ATGGAGGCAA GCCGACGGAA GAATACCCAA TGAATCCAAA TGGCTCTCCG 
TTGGGAATTG CTGGTCTTTG CTCTGCAGAT GGGCGGCACT TGGCCATGAT GCCCCACCCA 
GAGCGCTGTG TCATGAAGTG GCAGTGGCCT TGGATGCCGG AAAGCTGGCG CCACTCATTG 
GATGTCTCGC CGTGGATGCG TCTCTTCCAG AATGGGTACC GCTGGTGCCA GGAGAACCCA 
AGCAGGGATA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pfas ( XM_012970190.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012825644.1 Xenopus tropicalis phosphoribosylformylglycinamidine synthase (pfas), transcript variant X1, mRNA.

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CloneID	OXa32769
Clone ID Related Accession (Same CDS sequence)	NM_001142220.3 , XM_012970190.2 , XM_012970191.2
Accession Version	XM_012970190.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3975bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	phosphoribosylformylglycinamidine synthase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970190.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGTCC TGCACTTCTA TCGCTCCTTG CAGAATTCAT CAGAGACCCT GCGGCTGCCC 
GGTGCGGAGA ATATACAGAG CCTGCAGAGG GAGCTGTGCT ACAATGTCAA TTGGACAGGT 
CCCGCGGCCC CATCCAGCCA GGAGACTGAC GCCCTTCGAT GGCTGTTTAG TTGCCCCTTT 
GACCCTCAGA GTATCTCTGA CACCTCATCC CTTCACTCGG AGCCTTCTGA CCTGCTGGTA 
GAGATCGGCC CAAGACTGAA TTTCTCCACC GCGACTTCCA CCAATGCTGT GTCCATCTGC 
CGCTCTGTTG GCCTGACCAA CGTGGACAGG ATCGAGTGCT CCAAGAGGTA TCTCATCAAG 
TTCCAGACTA GGCCCCAGGG CAGTGAGGAG CAAGAGCTGC TAGCCTCTCT GTACGACCGT 
ATGACGGAAT GCGTTTACCC GGAGCCGGTG AGGAGTTTCG AGGTGTCCGT TAGACCGGAG 
AAAGTGTACG AGGTGGATAT TTTGGGGCAA GGCAGGGCAG CACTGGAAAA GGCCAACACT 
GAGCTAGGTT TGGCCTTCGA CTCCTGGGAT CTTGATTACT ATAGCAGCCT TTTCCAGCGC 
GTGGGTCGGA ACCCCAGCAG CGTGGAGTGC TTTGATCTTG CACAGTCCAA CAGCGAGCAC 
AGCCGCCACT GGTTCTTTAA GGGCAAATTA AAGGTCGACG GGCAGGAGAA GGAGATGTCT 
CTGTTCGACA TGATCATGAA GACTCAGGAC ACCAGTAATC CCAACAACGT CATCAAGTTC 
TGTGACAACA GCAGTGCGAT CCAGGGAAGA GAAGTGGCAT CTCTAATCCC AACAGACCCT 
TCATGTGCTG GGCCCTATCA TCTCTGTCGC TCCACACGGC ACCTGATTTT CACTGCAGAG 
ACCCACAACT TCCCTACTGG CGTGGCCCCA TTCAGCGGCG CTACGACAGG AACAGGAGGG 
CGCATTCGGG ACGTGCAGAG CACCGGCAGG GGAGCACATG TCATTGCTGG GACGGCTGGG 
TACTGCTTTG GGAACCTGCA CATCCCAGGA TACTCTCTTC CCTGGGAGGA CCTCGCATAT 
CAGTACCCAG TGCAGTTTGC CAGGCCTCTG GAGGTGGCTA TTGAAGCCAG TAACGGAGCC 
TCTGATTATG GAAACAAGTT TGGGGAGCCT GTTTTGGCCG GATTTGCCCG TTCCTTTGGC 
CTGCGGTTGC CTTCGGGAGA AAGGAGGGAG TGGGTGAAAC CCATTATGTT CAGTGGTGGG 
ATCGGATCCA TGGAGGATGT TCACCGTACT AAGGAGGCCC CAGAGCCTGG CATGCACGTG 
GTTAAAGTGG GAGGTCCTGT CTATAGGATT GGGGTTGGCG GTGGAGCTGC CTCTTCTATT 
CAGGTCCAGG GGGATAACGC CAGCGAGCTG GATTTTGGGG CAGTGCAACG TGGAGATGCA 
GAGATGGAGC AGAAGATGAA TCGGGCCGTA CGGGCCTGCG TGGAGCGAGG AGGCAAGAAC 
CCCATCTGCA GCATTCACGA TCAGGGAGCC GGGGGCAACG GGAACGTCCT GAAGGAGCTC 
AGTGAGCCCC AAGGAGCAGT CATTTACACT AAAAGCTTCC AGCTGGGGGA CCCCACCCTG 
AGTGTTCTGG AGATATGGGG AGCAGAATAT CAGGAGTCCA ACGCTTTGCT CCTGCGCCCC 
GACGATGCCG AATTCCTGCG CTCTGTGTGC CGGCGGGAGA GGTCTCCTGT GGATTTTGTG 
GGCAAGATCA CCGGGGATGG CAGGATTGTG TTGATAAATG GCAGTGACAC AGATCCCGCC 
CCTGACTCCA CTGACCGCAA TGCTGTTCCC GTTGACCTCC AGCTGGAATG GGTCTTAGGA 
AAAATGCCTC GCAAGGAGTT TGTTCTAAAC CGCGTGGCAC CAAACTTGCA GCCGCTGGTG 
CTCCCTAGTG GCCTGACAGT CGGGCAGGCG CTTGATCGCG TGCTGAGGTT GCCGTCCGTA 
GCGAGCAAAC GCTACCTGAC CAACAAGGTG GACCGCTCCG TCACAGGCCT GGTGGCCCAG 
CAGCAGTGCG TGGGGCCTCT TCACACTCCA TTGGCCGACG TGGCTGTGGT GTCTCTGTCT 
TATTCGGACA TTGTAGGAGG AGCTACAGCA ATAGGAGAAC AGCCAATCAA AAGCCTACTG 
AATCCTGCAG CTGGGGCAAG ACTGGCGGTG GCCGAGGCAC TAACCAATCT CCTCTTTGCT 
CAAGTGACGG ACCTAAAGGA TGTGAAATGC AGCGGGAACT GGATGTGGGC CGCCAAGCTG 
CCCGGAGAAG GCGCCTTGCT TTACGACGCC TGTGCAGCTA TGTGTGATGT CATGGCGCAG 
CTCGGCGTTG CCATCGATGG AGGAAAAGAT TCTCTCAGCA TGGCCGCCAG AGTGGGCACA 
GAAACAGTTA AAGCTCCAGG TTCGCTAGTG ATCTCGGTGT ATGCAGTCTG CCCCGACATA 
ACTACCACCG TGACCCCCGA TCTTAAGAAC CCAGGGCAGA AAGGTGTTTT ACTGTATTTG 
CCCCTCTGCC CTGGTCAGCA CCGGCTTGGG GGCAGCGCCC TGGCTCAGTG CTACTCACAG 
CTGGGAGAGA CCCCCCCAGA CCTCGATGAC CCTCAGACTT TAATCTCCTG CTTCAACGTC 
ACCCAACAGC TGCTGAGAGA CCGCGTCCTT AGCGCCGGCC ATGACGTCAG TGATGGAGGC 
CTAGTCACGT GTATCCTGGA AATGGCTTTT GCTGGAAACT GTGGCCTGGA TATTGAGATT 
TCCTCCTCCT GCACCAATGT GCTGGAACTG CTGTTTGCCG AGGAACCGGG CCTGGTTCTA 
GAGGTTGCAG AACAAAGTGT AGAACTGGTC ATGGAACGTT ACAGAGCAGC AGGAGTGGAA 
TGCGTGAGGA TCGGATGCAC CAAGGAACGT GGCCCAGCGT CTATGGTCCG AATCCGTGCC 
AATGGGCAGG AGGTGGTCAA CGAGAAGTTG GGTTCCCTCA GAGCCGTATG GGAGGAGACC 
AGTTTCCAAC TTGAACGCCT TCAGGCCAAT CCAAGCTGTG TATCGCAGGA GGAGGCTGGG 
CTGAGAGTGA GGGAGGGGCC AAGTTATCAC CTGACTTTTA ACCCGTCTGA AATACCTTTG 
GTTCCTCCAT CAGTCGGTGG CAACCAACCG CGTGTTGCTG TTGTGCGTGA AGAGGGTAGC 
AACGGAGACC GGGAAATGGC GGCATCTCTC CTAATGGCTG GATTTCAGGT GTGGGATGTC 
ACCATGGAAG ATCTTCTTGC TGGAGGCACC ACCCTGGACT CTTTCAGGGG ACTTGTATTT 
GTTGGGGGAT TTAGCTACGC TGATGTGTTA GGGTCTGCCA AAGGTTGGGC CGCCTCGGTG 
AAGTTCAATG CCAGCGTGCG CGAGCAGTTC GAGAACTTCC GCCGTAGGGC AGATACTTTC 
AGCCTTGGGG TTTGCAATGG GTGCCAGCTG ATGGCTCTTC TGGGCTGGGT AGGACCCGAT 
AACCCAACAG ACACAGTAGG TGATCTCCCC ACCCAAGGGG TTCTGCTCAG CCACAATCTC 
TCCGGCCGGT TTGAATCTCG ATTTGTCACC CTGAAGATCC AACAGAGTCC TTCCATTTTG 
CTACGAGGGA TGGCCGGGTC CACGCTCGGA GTGTGGGTGG CCCATGGCGA AGGATACATG 
AGGTTCCGAT CTCCGAAAGT GCAGGACTAT GTGACGTCCA ATCACTTGGC GCCGCTCTGT 
TACGTGGATG ATGGAGGCAA GCCGACGGAA GAATACCCAA TGAATCCAAA TGGCTCTCCG 
TTGGGAATTG CTGGTCTTTG CTCTGCAGAT GGGCGGCACT TGGCCATGAT GCCCCACCCA 
GAGCGCTGTG TCATGAAGTG GCAGTGGCCT TGGATGCCGG AAAGCTGGCG CCACTCATTG 
GATGTCTCGC CGTGGATGCG TCTCTTCCAG AATGGGTACC GCTGGTGCCA GGAGAACCCA 
AGCAGGGATA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012825644.1
CDS	105..4079
Translation	MVVLHFYRSLQNSSETLRLPGAENIQSLQRELCYNVNWTGPAAPSSQETDALRWLFSCPFDPQSISDTSSLHSEPSDLLVEIGPRLNFSTATSTNAVSICRSVGLTNVDRIECSKRYLIKFQTRPQGSEEQELLASLYDRMTECVYPEPVRSFEVSVRPEKVYEVDILGQGRAALEKANTELGLAFDSWDLDYYSSLFQRVGRNPSSVECFDLAQSNSEHSRHWFFKGKLKVDGQEKEMSLFDMIMKTQDTSNPNNVIKFCDNSSAIQGREVASLIPTDPSCAGPYHLCRSTRHLIFTAETHNFPTGVAPFSGATTGTGGRIRDVQSTGRGAHVIAGTAGYCFGNLHIPGYSLPWEDLAYQYPVQFARPLEVAIEASNGASDYGNKFGEPVLAGFARSFGLRLPSGERREWVKPIMFSGGIGSMEDVHRTKEAPEPGMHVVKVGGPVYRIGVGGGAASSIQVQGDNASELDFGAVQRGDAEMEQKMNRAVRACVERGGKNPICSIHDQGAGGNGNVLKELSEPQGAVIYTKSFQLGDPTLSVLEIWGAEYQESNALLLRPDDAEFLRSVCRRERSPVDFVGKITGDGRIVLINGSDTDPAPDSTDRNAVPVDLQLEWVLGKMPRKEFVLNRVAPNLQPLVLPSGLTVGQALDRVLRLPSVASKRYLTNKVDRSVTGLVAQQQCVGPLHTPLADVAVVSLSYSDIVGGATAIGEQPIKSLLNPAAGARLAVAEALTNLLFAQVTDLKDVKCSGNWMWAAKLPGEGALLYDACAAMCDVMAQLGVAIDGGKDSLSMAARVGTETVKAPGSLVISVYAVCPDITTTVTPDLKNPGQKGVLLYLPLCPGQHRLGGSALAQCYSQLGETPPDLDDPQTLISCFNVTQQLLRDRVLSAGHDVSDGGLVTCILEMAFAGNCGLDIEISSSCTNVLELLFAEEPGLVLEVAEQSVELVMERYRAAGVECVRIGCTKERGPASMVRIRANGQEVVNEKLGSLRAVWEETSFQLERLQANPSCVSQEEAGLRVREGPSYHLTFNPSEIPLVPPSVGGNQPRVAVVREEGSNGDREMAASLLMAGFQVWDVTMEDLLAGGTTLDSFRGLVFVGGFSYADVLGSAKGWAASVKFNASVREQFENFRRRADTFSLGVCNGCQLMALLGWVGPDNPTDTVGDLPTQGVLLSHNLSGRFESRFVTLKIQQSPSILLRGMAGSTLGVWVAHGEGYMRFRSPKVQDYVTSNHLAPLCYVDDGGKPTEEYPMNPNGSPLGIAGLCSADGRHLAMMPHPERCVMKWQWPWMPESWRHSLDVSPWMRLFQNGYRWCQENPSRDN

Target ORF information:

RefSeq Version	XM_012970190.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphoribosylformylglycinamidine synthase (pfas), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970190.2

ORF Insert Sequence:

ATGGTGGTCC TGCACTTCTA TCGCTCCTTG CAGAATTCAT CAGAGACCCT GCGGCTGCCC 
GGTGCGGAGA ATATACAGAG CCTGCAGAGG GAGCTGTGCT ACAATGTCAA TTGGACAGGT 
CCCGCGGCCC CATCCAGCCA GGAGACTGAC GCCCTTCGAT GGCTGTTTAG TTGCCCCTTT 
GACCCTCAGA GTATCTCTGA CACCTCATCC CTTCACTCGG AGCCTTCTGA CCTGCTGGTA 
GAGATCGGCC CAAGACTGAA TTTCTCCACC GCGACTTCCA CCAATGCTGT GTCCATCTGC 
CGCTCTGTTG GCCTGACCAA CGTGGACAGG ATCGAGTGCT CCAAGAGGTA TCTCATCAAG 
TTCCAGACTA GGCCCCAGGG CAGTGAGGAG CAAGAGCTGC TAGCCTCTCT GTACGACCGT 
ATGACGGAAT GCGTTTACCC GGAGCCGGTG AGGAGTTTCG AGGTGTCCGT TAGACCGGAG 
AAAGTGTACG AGGTGGATAT TTTGGGGCAA GGCAGGGCAG CACTGGAAAA GGCCAACACT 
GAGCTAGGTT TGGCCTTCGA CTCCTGGGAT CTTGATTACT ATAGCAGCCT TTTCCAGCGC 
GTGGGTCGGA ACCCCAGCAG CGTGGAGTGC TTTGATCTTG CACAGTCCAA CAGCGAGCAC 
AGCCGCCACT GGTTCTTTAA GGGCAAATTA AAGGTCGACG GGCAGGAGAA GGAGATGTCT 
CTGTTCGACA TGATCATGAA GACTCAGGAC ACCAGTAATC CCAACAACGT CATCAAGTTC 
TGTGACAACA GCAGTGCGAT CCAGGGAAGA GAAGTGGCAT CTCTAATCCC AACAGACCCT 
TCATGTGCTG GGCCCTATCA TCTCTGTCGC TCCACACGGC ACCTGATTTT CACTGCAGAG 
ACCCACAACT TCCCTACTGG CGTGGCCCCA TTCAGCGGCG CTACGACAGG AACAGGAGGG 
CGCATTCGGG ACGTGCAGAG CACCGGCAGG GGAGCACATG TCATTGCTGG GACGGCTGGG 
TACTGCTTTG GGAACCTGCA CATCCCAGGA TACTCTCTTC CCTGGGAGGA CCTCGCATAT 
CAGTACCCAG TGCAGTTTGC CAGGCCTCTG GAGGTGGCTA TTGAAGCCAG TAACGGAGCC 
TCTGATTATG GAAACAAGTT TGGGGAGCCT GTTTTGGCCG GATTTGCCCG TTCCTTTGGC 
CTGCGGTTGC CTTCGGGAGA AAGGAGGGAG TGGGTGAAAC CCATTATGTT CAGTGGTGGG 
ATCGGATCCA TGGAGGATGT TCACCGTACT AAGGAGGCCC CAGAGCCTGG CATGCACGTG 
GTTAAAGTGG GAGGTCCTGT CTATAGGATT GGGGTTGGCG GTGGAGCTGC CTCTTCTATT 
CAGGTCCAGG GGGATAACGC CAGCGAGCTG GATTTTGGGG CAGTGCAACG TGGAGATGCA 
GAGATGGAGC AGAAGATGAA TCGGGCCGTA CGGGCCTGCG TGGAGCGAGG AGGCAAGAAC 
CCCATCTGCA GCATTCACGA TCAGGGAGCC GGGGGCAACG GGAACGTCCT GAAGGAGCTC 
AGTGAGCCCC AAGGAGCAGT CATTTACACT AAAAGCTTCC AGCTGGGGGA CCCCACCCTG 
AGTGTTCTGG AGATATGGGG AGCAGAATAT CAGGAGTCCA ACGCTTTGCT CCTGCGCCCC 
GACGATGCCG AATTCCTGCG CTCTGTGTGC CGGCGGGAGA GGTCTCCTGT GGATTTTGTG 
GGCAAGATCA CCGGGGATGG CAGGATTGTG TTGATAAATG GCAGTGACAC AGATCCCGCC 
CCTGACTCCA CTGACCGCAA TGCTGTTCCC GTTGACCTCC AGCTGGAATG GGTCTTAGGA 
AAAATGCCTC GCAAGGAGTT TGTTCTAAAC CGCGTGGCAC CAAACTTGCA GCCGCTGGTG 
CTCCCTAGTG GCCTGACAGT CGGGCAGGCG CTTGATCGCG TGCTGAGGTT GCCGTCCGTA 
GCGAGCAAAC GCTACCTGAC CAACAAGGTG GACCGCTCCG TCACAGGCCT GGTGGCCCAG 
CAGCAGTGCG TGGGGCCTCT TCACACTCCA TTGGCCGACG TGGCTGTGGT GTCTCTGTCT 
TATTCGGACA TTGTAGGAGG AGCTACAGCA ATAGGAGAAC AGCCAATCAA AAGCCTACTG 
AATCCTGCAG CTGGGGCAAG ACTGGCGGTG GCCGAGGCAC TAACCAATCT CCTCTTTGCT 
CAAGTGACGG ACCTAAAGGA TGTGAAATGC AGCGGGAACT GGATGTGGGC CGCCAAGCTG 
CCCGGAGAAG GCGCCTTGCT TTACGACGCC TGTGCAGCTA TGTGTGATGT CATGGCGCAG 
CTCGGCGTTG CCATCGATGG AGGAAAAGAT TCTCTCAGCA TGGCCGCCAG AGTGGGCACA 
GAAACAGTTA AAGCTCCAGG TTCGCTAGTG ATCTCGGTGT ATGCAGTCTG CCCCGACATA 
ACTACCACCG TGACCCCCGA TCTTAAGAAC CCAGGGCAGA AAGGTGTTTT ACTGTATTTG 
CCCCTCTGCC CTGGTCAGCA CCGGCTTGGG GGCAGCGCCC TGGCTCAGTG CTACTCACAG 
CTGGGAGAGA CCCCCCCAGA CCTCGATGAC CCTCAGACTT TAATCTCCTG CTTCAACGTC 
ACCCAACAGC TGCTGAGAGA CCGCGTCCTT AGCGCCGGCC ATGACGTCAG TGATGGAGGC 
CTAGTCACGT GTATCCTGGA AATGGCTTTT GCTGGAAACT GTGGCCTGGA TATTGAGATT 
TCCTCCTCCT GCACCAATGT GCTGGAACTG CTGTTTGCCG AGGAACCGGG CCTGGTTCTA 
GAGGTTGCAG AACAAAGTGT AGAACTGGTC ATGGAACGTT ACAGAGCAGC AGGAGTGGAA 
TGCGTGAGGA TCGGATGCAC CAAGGAACGT GGCCCAGCGT CTATGGTCCG AATCCGTGCC 
AATGGGCAGG AGGTGGTCAA CGAGAAGTTG GGTTCCCTCA GAGCCGTATG GGAGGAGACC 
AGTTTCCAAC TTGAACGCCT TCAGGCCAAT CCAAGCTGTG TATCGCAGGA GGAGGCTGGG 
CTGAGAGTGA GGGAGGGGCC AAGTTATCAC CTGACTTTTA ACCCGTCTGA AATACCTTTG 
GTTCCTCCAT CAGTCGGTGG CAACCAACCG CGTGTTGCTG TTGTGCGTGA AGAGGGTAGC 
AACGGAGACC GGGAAATGGC GGCATCTCTC CTAATGGCTG GATTTCAGGT GTGGGATGTC 
ACCATGGAAG ATCTTCTTGC TGGAGGCACC ACCCTGGACT CTTTCAGGGG ACTTGTATTT 
GTTGGGGGAT TTAGCTACGC TGATGTGTTA GGGTCTGCCA AAGGTTGGGC CGCCTCGGTG 
AAGTTCAATG CCAGCGTGCG CGAGCAGTTC GAGAACTTCC GCCGTAGGGC AGATACTTTC 
AGCCTTGGGG TTTGCAATGG GTGCCAGCTG ATGGCTCTTC TGGGCTGGGT AGGACCCGAT 
AACCCAACAG ACACAGTAGG TGATCTCCCC ACCCAAGGGG TTCTGCTCAG CCACAATCTC 
TCCGGCCGGT TTGAATCTCG ATTTGTCACC CTGAAGATCC AACAGAGTCC TTCCATTTTG 
CTACGAGGGA TGGCCGGGTC CACGCTCGGA GTGTGGGTGG CCCATGGCGA AGGATACATG 
AGGTTCCGAT CTCCGAAAGT GCAGGACTAT GTGACGTCCA ATCACTTGGC GCCGCTCTGT 
TACGTGGATG ATGGAGGCAA GCCGACGGAA GAATACCCAA TGAATCCAAA TGGCTCTCCG 
TTGGGAATTG CTGGTCTTTG CTCTGCAGAT GGGCGGCACT TGGCCATGAT GCCCCACCCA 
GAGCGCTGTG TCATGAAGTG GCAGTGGCCT TGGATGCCGG AAAGCTGGCG CCACTCATTG 
GATGTCTCGC CGTGGATGCG TCTCTTCCAG AATGGGTACC GCTGGTGCCA GGAGAACCCA 
AGCAGGGATA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pfas ( XM_012970191.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012825645.1 Xenopus tropicalis phosphoribosylformylglycinamidine synthase (pfas), transcript variant X2, mRNA.

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CloneID	OXa32769
Clone ID Related Accession (Same CDS sequence)	NM_001142220.3 , XM_012970190.2 , XM_012970191.2
Accession Version	XM_012970191.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3975bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	phosphoribosylformylglycinamidine synthase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970191.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGTCC TGCACTTCTA TCGCTCCTTG CAGAATTCAT CAGAGACCCT GCGGCTGCCC 
GGTGCGGAGA ATATACAGAG CCTGCAGAGG GAGCTGTGCT ACAATGTCAA TTGGACAGGT 
CCCGCGGCCC CATCCAGCCA GGAGACTGAC GCCCTTCGAT GGCTGTTTAG TTGCCCCTTT 
GACCCTCAGA GTATCTCTGA CACCTCATCC CTTCACTCGG AGCCTTCTGA CCTGCTGGTA 
GAGATCGGCC CAAGACTGAA TTTCTCCACC GCGACTTCCA CCAATGCTGT GTCCATCTGC 
CGCTCTGTTG GCCTGACCAA CGTGGACAGG ATCGAGTGCT CCAAGAGGTA TCTCATCAAG 
TTCCAGACTA GGCCCCAGGG CAGTGAGGAG CAAGAGCTGC TAGCCTCTCT GTACGACCGT 
ATGACGGAAT GCGTTTACCC GGAGCCGGTG AGGAGTTTCG AGGTGTCCGT TAGACCGGAG 
AAAGTGTACG AGGTGGATAT TTTGGGGCAA GGCAGGGCAG CACTGGAAAA GGCCAACACT 
GAGCTAGGTT TGGCCTTCGA CTCCTGGGAT CTTGATTACT ATAGCAGCCT TTTCCAGCGC 
GTGGGTCGGA ACCCCAGCAG CGTGGAGTGC TTTGATCTTG CACAGTCCAA CAGCGAGCAC 
AGCCGCCACT GGTTCTTTAA GGGCAAATTA AAGGTCGACG GGCAGGAGAA GGAGATGTCT 
CTGTTCGACA TGATCATGAA GACTCAGGAC ACCAGTAATC CCAACAACGT CATCAAGTTC 
TGTGACAACA GCAGTGCGAT CCAGGGAAGA GAAGTGGCAT CTCTAATCCC AACAGACCCT 
TCATGTGCTG GGCCCTATCA TCTCTGTCGC TCCACACGGC ACCTGATTTT CACTGCAGAG 
ACCCACAACT TCCCTACTGG CGTGGCCCCA TTCAGCGGCG CTACGACAGG AACAGGAGGG 
CGCATTCGGG ACGTGCAGAG CACCGGCAGG GGAGCACATG TCATTGCTGG GACGGCTGGG 
TACTGCTTTG GGAACCTGCA CATCCCAGGA TACTCTCTTC CCTGGGAGGA CCTCGCATAT 
CAGTACCCAG TGCAGTTTGC CAGGCCTCTG GAGGTGGCTA TTGAAGCCAG TAACGGAGCC 
TCTGATTATG GAAACAAGTT TGGGGAGCCT GTTTTGGCCG GATTTGCCCG TTCCTTTGGC 
CTGCGGTTGC CTTCGGGAGA AAGGAGGGAG TGGGTGAAAC CCATTATGTT CAGTGGTGGG 
ATCGGATCCA TGGAGGATGT TCACCGTACT AAGGAGGCCC CAGAGCCTGG CATGCACGTG 
GTTAAAGTGG GAGGTCCTGT CTATAGGATT GGGGTTGGCG GTGGAGCTGC CTCTTCTATT 
CAGGTCCAGG GGGATAACGC CAGCGAGCTG GATTTTGGGG CAGTGCAACG TGGAGATGCA 
GAGATGGAGC AGAAGATGAA TCGGGCCGTA CGGGCCTGCG TGGAGCGAGG AGGCAAGAAC 
CCCATCTGCA GCATTCACGA TCAGGGAGCC GGGGGCAACG GGAACGTCCT GAAGGAGCTC 
AGTGAGCCCC AAGGAGCAGT CATTTACACT AAAAGCTTCC AGCTGGGGGA CCCCACCCTG 
AGTGTTCTGG AGATATGGGG AGCAGAATAT CAGGAGTCCA ACGCTTTGCT CCTGCGCCCC 
GACGATGCCG AATTCCTGCG CTCTGTGTGC CGGCGGGAGA GGTCTCCTGT GGATTTTGTG 
GGCAAGATCA CCGGGGATGG CAGGATTGTG TTGATAAATG GCAGTGACAC AGATCCCGCC 
CCTGACTCCA CTGACCGCAA TGCTGTTCCC GTTGACCTCC AGCTGGAATG GGTCTTAGGA 
AAAATGCCTC GCAAGGAGTT TGTTCTAAAC CGCGTGGCAC CAAACTTGCA GCCGCTGGTG 
CTCCCTAGTG GCCTGACAGT CGGGCAGGCG CTTGATCGCG TGCTGAGGTT GCCGTCCGTA 
GCGAGCAAAC GCTACCTGAC CAACAAGGTG GACCGCTCCG TCACAGGCCT GGTGGCCCAG 
CAGCAGTGCG TGGGGCCTCT TCACACTCCA TTGGCCGACG TGGCTGTGGT GTCTCTGTCT 
TATTCGGACA TTGTAGGAGG AGCTACAGCA ATAGGAGAAC AGCCAATCAA AAGCCTACTG 
AATCCTGCAG CTGGGGCAAG ACTGGCGGTG GCCGAGGCAC TAACCAATCT CCTCTTTGCT 
CAAGTGACGG ACCTAAAGGA TGTGAAATGC AGCGGGAACT GGATGTGGGC CGCCAAGCTG 
CCCGGAGAAG GCGCCTTGCT TTACGACGCC TGTGCAGCTA TGTGTGATGT CATGGCGCAG 
CTCGGCGTTG CCATCGATGG AGGAAAAGAT TCTCTCAGCA TGGCCGCCAG AGTGGGCACA 
GAAACAGTTA AAGCTCCAGG TTCGCTAGTG ATCTCGGTGT ATGCAGTCTG CCCCGACATA 
ACTACCACCG TGACCCCCGA TCTTAAGAAC CCAGGGCAGA AAGGTGTTTT ACTGTATTTG 
CCCCTCTGCC CTGGTCAGCA CCGGCTTGGG GGCAGCGCCC TGGCTCAGTG CTACTCACAG 
CTGGGAGAGA CCCCCCCAGA CCTCGATGAC CCTCAGACTT TAATCTCCTG CTTCAACGTC 
ACCCAACAGC TGCTGAGAGA CCGCGTCCTT AGCGCCGGCC ATGACGTCAG TGATGGAGGC 
CTAGTCACGT GTATCCTGGA AATGGCTTTT GCTGGAAACT GTGGCCTGGA TATTGAGATT 
TCCTCCTCCT GCACCAATGT GCTGGAACTG CTGTTTGCCG AGGAACCGGG CCTGGTTCTA 
GAGGTTGCAG AACAAAGTGT AGAACTGGTC ATGGAACGTT ACAGAGCAGC AGGAGTGGAA 
TGCGTGAGGA TCGGATGCAC CAAGGAACGT GGCCCAGCGT CTATGGTCCG AATCCGTGCC 
AATGGGCAGG AGGTGGTCAA CGAGAAGTTG GGTTCCCTCA GAGCCGTATG GGAGGAGACC 
AGTTTCCAAC TTGAACGCCT TCAGGCCAAT CCAAGCTGTG TATCGCAGGA GGAGGCTGGG 
CTGAGAGTGA GGGAGGGGCC AAGTTATCAC CTGACTTTTA ACCCGTCTGA AATACCTTTG 
GTTCCTCCAT CAGTCGGTGG CAACCAACCG CGTGTTGCTG TTGTGCGTGA AGAGGGTAGC 
AACGGAGACC GGGAAATGGC GGCATCTCTC CTAATGGCTG GATTTCAGGT GTGGGATGTC 
ACCATGGAAG ATCTTCTTGC TGGAGGCACC ACCCTGGACT CTTTCAGGGG ACTTGTATTT 
GTTGGGGGAT TTAGCTACGC TGATGTGTTA GGGTCTGCCA AAGGTTGGGC CGCCTCGGTG 
AAGTTCAATG CCAGCGTGCG CGAGCAGTTC GAGAACTTCC GCCGTAGGGC AGATACTTTC 
AGCCTTGGGG TTTGCAATGG GTGCCAGCTG ATGGCTCTTC TGGGCTGGGT AGGACCCGAT 
AACCCAACAG ACACAGTAGG TGATCTCCCC ACCCAAGGGG TTCTGCTCAG CCACAATCTC 
TCCGGCCGGT TTGAATCTCG ATTTGTCACC CTGAAGATCC AACAGAGTCC TTCCATTTTG 
CTACGAGGGA TGGCCGGGTC CACGCTCGGA GTGTGGGTGG CCCATGGCGA AGGATACATG 
AGGTTCCGAT CTCCGAAAGT GCAGGACTAT GTGACGTCCA ATCACTTGGC GCCGCTCTGT 
TACGTGGATG ATGGAGGCAA GCCGACGGAA GAATACCCAA TGAATCCAAA TGGCTCTCCG 
TTGGGAATTG CTGGTCTTTG CTCTGCAGAT GGGCGGCACT TGGCCATGAT GCCCCACCCA 
GAGCGCTGTG TCATGAAGTG GCAGTGGCCT TGGATGCCGG AAAGCTGGCG CCACTCATTG 
GATGTCTCGC CGTGGATGCG TCTCTTCCAG AATGGGTACC GCTGGTGCCA GGAGAACCCA 
AGCAGGGATA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012825645.1
CDS	43..4017
Translation	MVVLHFYRSLQNSSETLRLPGAENIQSLQRELCYNVNWTGPAAPSSQETDALRWLFSCPFDPQSISDTSSLHSEPSDLLVEIGPRLNFSTATSTNAVSICRSVGLTNVDRIECSKRYLIKFQTRPQGSEEQELLASLYDRMTECVYPEPVRSFEVSVRPEKVYEVDILGQGRAALEKANTELGLAFDSWDLDYYSSLFQRVGRNPSSVECFDLAQSNSEHSRHWFFKGKLKVDGQEKEMSLFDMIMKTQDTSNPNNVIKFCDNSSAIQGREVASLIPTDPSCAGPYHLCRSTRHLIFTAETHNFPTGVAPFSGATTGTGGRIRDVQSTGRGAHVIAGTAGYCFGNLHIPGYSLPWEDLAYQYPVQFARPLEVAIEASNGASDYGNKFGEPVLAGFARSFGLRLPSGERREWVKPIMFSGGIGSMEDVHRTKEAPEPGMHVVKVGGPVYRIGVGGGAASSIQVQGDNASELDFGAVQRGDAEMEQKMNRAVRACVERGGKNPICSIHDQGAGGNGNVLKELSEPQGAVIYTKSFQLGDPTLSVLEIWGAEYQESNALLLRPDDAEFLRSVCRRERSPVDFVGKITGDGRIVLINGSDTDPAPDSTDRNAVPVDLQLEWVLGKMPRKEFVLNRVAPNLQPLVLPSGLTVGQALDRVLRLPSVASKRYLTNKVDRSVTGLVAQQQCVGPLHTPLADVAVVSLSYSDIVGGATAIGEQPIKSLLNPAAGARLAVAEALTNLLFAQVTDLKDVKCSGNWMWAAKLPGEGALLYDACAAMCDVMAQLGVAIDGGKDSLSMAARVGTETVKAPGSLVISVYAVCPDITTTVTPDLKNPGQKGVLLYLPLCPGQHRLGGSALAQCYSQLGETPPDLDDPQTLISCFNVTQQLLRDRVLSAGHDVSDGGLVTCILEMAFAGNCGLDIEISSSCTNVLELLFAEEPGLVLEVAEQSVELVMERYRAAGVECVRIGCTKERGPASMVRIRANGQEVVNEKLGSLRAVWEETSFQLERLQANPSCVSQEEAGLRVREGPSYHLTFNPSEIPLVPPSVGGNQPRVAVVREEGSNGDREMAASLLMAGFQVWDVTMEDLLAGGTTLDSFRGLVFVGGFSYADVLGSAKGWAASVKFNASVREQFENFRRRADTFSLGVCNGCQLMALLGWVGPDNPTDTVGDLPTQGVLLSHNLSGRFESRFVTLKIQQSPSILLRGMAGSTLGVWVAHGEGYMRFRSPKVQDYVTSNHLAPLCYVDDGGKPTEEYPMNPNGSPLGIAGLCSADGRHLAMMPHPERCVMKWQWPWMPESWRHSLDVSPWMRLFQNGYRWCQENPSRDN

Target ORF information:

RefSeq Version	XM_012970191.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphoribosylformylglycinamidine synthase (pfas), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970191.2

ORF Insert Sequence:

ATGGTGGTCC TGCACTTCTA TCGCTCCTTG CAGAATTCAT CAGAGACCCT GCGGCTGCCC 
GGTGCGGAGA ATATACAGAG CCTGCAGAGG GAGCTGTGCT ACAATGTCAA TTGGACAGGT 
CCCGCGGCCC CATCCAGCCA GGAGACTGAC GCCCTTCGAT GGCTGTTTAG TTGCCCCTTT 
GACCCTCAGA GTATCTCTGA CACCTCATCC CTTCACTCGG AGCCTTCTGA CCTGCTGGTA 
GAGATCGGCC CAAGACTGAA TTTCTCCACC GCGACTTCCA CCAATGCTGT GTCCATCTGC 
CGCTCTGTTG GCCTGACCAA CGTGGACAGG ATCGAGTGCT CCAAGAGGTA TCTCATCAAG 
TTCCAGACTA GGCCCCAGGG CAGTGAGGAG CAAGAGCTGC TAGCCTCTCT GTACGACCGT 
ATGACGGAAT GCGTTTACCC GGAGCCGGTG AGGAGTTTCG AGGTGTCCGT TAGACCGGAG 
AAAGTGTACG AGGTGGATAT TTTGGGGCAA GGCAGGGCAG CACTGGAAAA GGCCAACACT 
GAGCTAGGTT TGGCCTTCGA CTCCTGGGAT CTTGATTACT ATAGCAGCCT TTTCCAGCGC 
GTGGGTCGGA ACCCCAGCAG CGTGGAGTGC TTTGATCTTG CACAGTCCAA CAGCGAGCAC 
AGCCGCCACT GGTTCTTTAA GGGCAAATTA AAGGTCGACG GGCAGGAGAA GGAGATGTCT 
CTGTTCGACA TGATCATGAA GACTCAGGAC ACCAGTAATC CCAACAACGT CATCAAGTTC 
TGTGACAACA GCAGTGCGAT CCAGGGAAGA GAAGTGGCAT CTCTAATCCC AACAGACCCT 
TCATGTGCTG GGCCCTATCA TCTCTGTCGC TCCACACGGC ACCTGATTTT CACTGCAGAG 
ACCCACAACT TCCCTACTGG CGTGGCCCCA TTCAGCGGCG CTACGACAGG AACAGGAGGG 
CGCATTCGGG ACGTGCAGAG CACCGGCAGG GGAGCACATG TCATTGCTGG GACGGCTGGG 
TACTGCTTTG GGAACCTGCA CATCCCAGGA TACTCTCTTC CCTGGGAGGA CCTCGCATAT 
CAGTACCCAG TGCAGTTTGC CAGGCCTCTG GAGGTGGCTA TTGAAGCCAG TAACGGAGCC 
TCTGATTATG GAAACAAGTT TGGGGAGCCT GTTTTGGCCG GATTTGCCCG TTCCTTTGGC 
CTGCGGTTGC CTTCGGGAGA AAGGAGGGAG TGGGTGAAAC CCATTATGTT CAGTGGTGGG 
ATCGGATCCA TGGAGGATGT TCACCGTACT AAGGAGGCCC CAGAGCCTGG CATGCACGTG 
GTTAAAGTGG GAGGTCCTGT CTATAGGATT GGGGTTGGCG GTGGAGCTGC CTCTTCTATT 
CAGGTCCAGG GGGATAACGC CAGCGAGCTG GATTTTGGGG CAGTGCAACG TGGAGATGCA 
GAGATGGAGC AGAAGATGAA TCGGGCCGTA CGGGCCTGCG TGGAGCGAGG AGGCAAGAAC 
CCCATCTGCA GCATTCACGA TCAGGGAGCC GGGGGCAACG GGAACGTCCT GAAGGAGCTC 
AGTGAGCCCC AAGGAGCAGT CATTTACACT AAAAGCTTCC AGCTGGGGGA CCCCACCCTG 
AGTGTTCTGG AGATATGGGG AGCAGAATAT CAGGAGTCCA ACGCTTTGCT CCTGCGCCCC 
GACGATGCCG AATTCCTGCG CTCTGTGTGC CGGCGGGAGA GGTCTCCTGT GGATTTTGTG 
GGCAAGATCA CCGGGGATGG CAGGATTGTG TTGATAAATG GCAGTGACAC AGATCCCGCC 
CCTGACTCCA CTGACCGCAA TGCTGTTCCC GTTGACCTCC AGCTGGAATG GGTCTTAGGA 
AAAATGCCTC GCAAGGAGTT TGTTCTAAAC CGCGTGGCAC CAAACTTGCA GCCGCTGGTG 
CTCCCTAGTG GCCTGACAGT CGGGCAGGCG CTTGATCGCG TGCTGAGGTT GCCGTCCGTA 
GCGAGCAAAC GCTACCTGAC CAACAAGGTG GACCGCTCCG TCACAGGCCT GGTGGCCCAG 
CAGCAGTGCG TGGGGCCTCT TCACACTCCA TTGGCCGACG TGGCTGTGGT GTCTCTGTCT 
TATTCGGACA TTGTAGGAGG AGCTACAGCA ATAGGAGAAC AGCCAATCAA AAGCCTACTG 
AATCCTGCAG CTGGGGCAAG ACTGGCGGTG GCCGAGGCAC TAACCAATCT CCTCTTTGCT 
CAAGTGACGG ACCTAAAGGA TGTGAAATGC AGCGGGAACT GGATGTGGGC CGCCAAGCTG 
CCCGGAGAAG GCGCCTTGCT TTACGACGCC TGTGCAGCTA TGTGTGATGT CATGGCGCAG 
CTCGGCGTTG CCATCGATGG AGGAAAAGAT TCTCTCAGCA TGGCCGCCAG AGTGGGCACA 
GAAACAGTTA AAGCTCCAGG TTCGCTAGTG ATCTCGGTGT ATGCAGTCTG CCCCGACATA 
ACTACCACCG TGACCCCCGA TCTTAAGAAC CCAGGGCAGA AAGGTGTTTT ACTGTATTTG 
CCCCTCTGCC CTGGTCAGCA CCGGCTTGGG GGCAGCGCCC TGGCTCAGTG CTACTCACAG 
CTGGGAGAGA CCCCCCCAGA CCTCGATGAC CCTCAGACTT TAATCTCCTG CTTCAACGTC 
ACCCAACAGC TGCTGAGAGA CCGCGTCCTT AGCGCCGGCC ATGACGTCAG TGATGGAGGC 
CTAGTCACGT GTATCCTGGA AATGGCTTTT GCTGGAAACT GTGGCCTGGA TATTGAGATT 
TCCTCCTCCT GCACCAATGT GCTGGAACTG CTGTTTGCCG AGGAACCGGG CCTGGTTCTA 
GAGGTTGCAG AACAAAGTGT AGAACTGGTC ATGGAACGTT ACAGAGCAGC AGGAGTGGAA 
TGCGTGAGGA TCGGATGCAC CAAGGAACGT GGCCCAGCGT CTATGGTCCG AATCCGTGCC 
AATGGGCAGG AGGTGGTCAA CGAGAAGTTG GGTTCCCTCA GAGCCGTATG GGAGGAGACC 
AGTTTCCAAC TTGAACGCCT TCAGGCCAAT CCAAGCTGTG TATCGCAGGA GGAGGCTGGG 
CTGAGAGTGA GGGAGGGGCC AAGTTATCAC CTGACTTTTA ACCCGTCTGA AATACCTTTG 
GTTCCTCCAT CAGTCGGTGG CAACCAACCG CGTGTTGCTG TTGTGCGTGA AGAGGGTAGC 
AACGGAGACC GGGAAATGGC GGCATCTCTC CTAATGGCTG GATTTCAGGT GTGGGATGTC 
ACCATGGAAG ATCTTCTTGC TGGAGGCACC ACCCTGGACT CTTTCAGGGG ACTTGTATTT 
GTTGGGGGAT TTAGCTACGC TGATGTGTTA GGGTCTGCCA AAGGTTGGGC CGCCTCGGTG 
AAGTTCAATG CCAGCGTGCG CGAGCAGTTC GAGAACTTCC GCCGTAGGGC AGATACTTTC 
AGCCTTGGGG TTTGCAATGG GTGCCAGCTG ATGGCTCTTC TGGGCTGGGT AGGACCCGAT 
AACCCAACAG ACACAGTAGG TGATCTCCCC ACCCAAGGGG TTCTGCTCAG CCACAATCTC 
TCCGGCCGGT TTGAATCTCG ATTTGTCACC CTGAAGATCC AACAGAGTCC TTCCATTTTG 
CTACGAGGGA TGGCCGGGTC CACGCTCGGA GTGTGGGTGG CCCATGGCGA AGGATACATG 
AGGTTCCGAT CTCCGAAAGT GCAGGACTAT GTGACGTCCA ATCACTTGGC GCCGCTCTGT 
TACGTGGATG ATGGAGGCAA GCCGACGGAA GAATACCCAA TGAATCCAAA TGGCTCTCCG 
TTGGGAATTG CTGGTCTTTG CTCTGCAGAT GGGCGGCACT TGGCCATGAT GCCCCACCCA 
GAGCGCTGTG TCATGAAGTG GCAGTGGCCT TGGATGCCGG AAAGCTGGCG CCACTCATTG 
GATGTCTCGC CGTGGATGCG TCTCTTCCAG AATGGGTACC GCTGGTGCCA GGAGAACCCA 
AGCAGGGATA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pfas ( XM_012970192.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012825646.1 Xenopus tropicalis phosphoribosylformylglycinamidine synthase (pfas), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa32770
Clone ID Related Accession (Same CDS sequence)	XM_012970192.2
Accession Version	XM_012970192.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	phosphoribosylformylglycinamidine synthase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970192.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGTCC TGCACTTCTA TCGCTCCTTG CAGAATTCAT CAGAGACCCT GCGGCTGCCC 
GGTGCGGAGA ATATACAGAG CCTGCAGAGG GAGCTGTGCT ACAATGTCAA TTGGACAGGT 
CCCGCGGCCC CATCCAGCCA GGAGACTGAC GCCCTTCGAT GGCTGTTTAG TTGCCCCTTT 
GACCCTCAGA GTATCTCTGA CACCTCATCC CTTCACTCGG AGCCTTCTGA CCTGCTGGTA 
GAGATCGGCC CAAGACTGAA TTTCTCCACC GCGACTTCCA CCAATGCTGT GTCCATCTGC 
CGCTCTGTTG GCCTGACCAA CGTGGACAGG ATCGAGTGCT CCAAGAGGTA TCTCATCAAG 
TTCCAGACTA GGCCCCAGGG CAGTGAGGAG CAAGAGCTGC TAGCCTCTCT GTACGACCGT 
ATGACGGAAT GCGTTTACCC GGAGCCGGTG AGGAGTTTCG AGGTGTCCGT TAGACCGGAG 
AAAGTGTACG AGGTGGATAT TTTGGGGCAA GGCAGGGCAG CACTGGAAAA GGCCAACACT 
GAGCTAGGTT TGGCCTTCGA CTCCTGGGAT CTTGATTACT ATAGCAGCCT TTTCCAGCGC 
GTGGGTCGGA ACCCCAGCAG CGTGGAGTGC TTTGATCTTG CACAGTCCAA CAGCGAGCAC 
AGCCGCCACT GGTTCTTTAA GGGCAAATTA AAGGTCGACG GGCAGGAGAA GGAGATGTCT 
CTGTTCGACA TGATCATGAA GACTCAGGAC ACCAGTAATC CCAACAACGT CATCAAGTTC 
TGTGACAACA GCAGTGCGAT CCAGGGAAGA GAAGTGGCAT CTCTAATCCC AACAGACCCT 
TCATGTGCTG GGCCCTATCA TCTCTGTCGC TCCACACGGC ACCTGATTTT CACTGCAGAG 
ACCCACAACT TCCCTACTGG CGTGGCCCCA TTCAGCGGCG CTACGACAGG AACAGGAGGG 
CGCATTCGGG ACGTGCAGAG CACCGGCAGG GGAGCACATG TCATTGCTGG GACGGCTGGG 
TACTGCTTTG GGAACCTGCA CATCCCAGGA TACTCTCTTC CCTGGGAGGA CCTCGCATAT 
CAGTACCCAG TGCAGTTTGC CAGGCCTCTG GAGGTGGCTA TTGAAGCCAG TAACGGAGCC 
TCTGATTATG GAAACAAGTT TGGGGAGCCT GTTTTGGCCG GATTTGCCCG TTCCTTTGGC 
CTGCGGTTGC CTTCGGGAGA AAGGAGGGAG TGGGTGAAAC CCATTATGTT CAGTGGTGGG 
ATCGGATCCA TGGAGGATGT TCACCGTACT AAGGAGGCCC CAGAGCCTGG CATGCACGTG 
GTTAAAGTGG GAGGTCCTGT CTATAGGATT GGGGTTGGCG GTGGAGCTGC CTCTTCTATT 
CAGGTCCAGG GGGATAACGC CAGCGAGCTG GATTTTGGGG CAGTGCAACG TGGAGATGCA 
GAGATGGAGC AGAAGATGAA TCGGGCCGTA CGGGCCTGCG TGGAGCGAGG AGGCAAGAAC 
CCCATCTGCA GCATTCACGA TCAGGGAGCC GGGGGCAACG GGAACGTCCT GAAGGAGCTC 
AGTGAGCCCC AAGGAGCAGT CATTTACACT AAAAGCTTCC AGCTGGGGGA CCCCACCCTG 
AGTGTTCTGG AGATATGGGG AGCAGAATAT CAGGAGTCCA ACGCTTTGCT CCTGCGCCCC 
GACGATGCCG AATTCCTGCG CTCTGTGTGC CGGCGGGAGA GGTCTCCTGT GGATTTTGTG 
GGCAAGATCA CCGGGGATGG CAGGATTGTG TTGATAAATG GCAGTGACAC AGATCCCGCC 
CCTGACTCCA CTGACCGCAA TGCTGTTCCC GTTGACCTCC AGCTGGAATG GGTCTTAGGA 
AAAATGCCTC GCAAGGAGTT TGTTCTAAAC CGCGTGGCAC CAAACTTGCA GCCGCTGGTG 
CTCCCTAGTG GCCTGACAGT CGGGCAGGCG CTTGATCGCG TGCTGAGGTT GCCGTCCGTA 
GCGAGCAAAC GCTACCTGAC CAACAAGGTG GACCGCTCCG TCACAGGCCT GGTGGCCCAG 
CAGCAGTGCG TGGGGCCTCT TCACACTCCA TTGGCCGACG TGGCTGTGGT GTCTCTGTCT 
TATTCGGACA TTGTAGGAGG AGCTACAGCA ATAGGAGAAC AGCCAATCAA AAGCCTACTG 
AATCCTGCAG CTGGGGCAAG ACTGGCGGTG GCCGAGGCAC TAACCAATCT CCTCTTTGCT 
CAAGTGACGG ACCTAAAGGA TGTGAAATGC AGCGGGAACT GGATGTGGGC CGCCAAGCTG 
CCCGGAGAAG GCGCCTTGCT TTACGACGCC TGTGCAGCTA TGTGTGATGT CATGGCGCAG 
CTCGGCGTTG CCATCGATGG AGGAAAAGAT TCTCTCAGCA TGGCCGCCAG AGTGGGCACA 
GAAACAGTTA AAGCTCCAGG TTCGCTAGTG ATCTCGGTGT ATGCAGTCTG CCCCGACATA 
ACTACCACCG TGACCCCCGA TCTTAAGAAC CCAGGGCAGA AAGGTGTTTT ACTGTATTTG 
CCCCTCTGCC CTGGTCAGCA CCGGCTTGGG GGCAGCGCCC TGGCTCAGTG CTACTCACAG 
CTGGGAGAGA CCCCCCCAGA CCTCGATGAC CCTCAGACTT TAATCTCCTG CTTCAACGTC 
ACCCAACAGC TGCTGAGAGA CCGCGTCCTT AGCGCCGGCC ATGACGTCAG TGATGGAGGC 
CTAGTCACGT GTATCCTGGA AATGGCTTTT GCTGGAAACT GTGGCCTGGA TATTGAGATT 
TCCTCCTCCT GCACCAATGT GCTGGAACTG CTGTTTGCCG AGGAACCGGG CCTGGTTCTA 
GAGGTTGCAG AACAAAGTGT AGAACTGGTC ATGGAACGTT ACAGAGCAGC AGGAGTGGAA 
TGCGTGAGGA TCGGATGCAC CAAGGAACGT GGCCCAGCGT CTATGGTCCG AATCCGTGCC 
AATGGGCAGG AGGTGGTCAA CGAGAAGTTG GGTTCCCTCA GAGCCGTATG GGAGGAGACC 
AGTTTCCAAC TTGAACGCCT TCAGGCCAAT CCAAGCTGTG TATCGCAGGA GGAGGCTGGG 
CTGAGAGTGA GGGAGGGGCC AAGTTATCAC CTGACTTTTA ACCCGTCTGA AATACCTTTG 
GTTCCTCCAT CAGTCGGTGG CAACCAACCG CGTGTTGCTG TTGTGCGTGA AGAGGGTAGC 
AACGGAGACC GGGAAATGGC GGCATCTCTC CTAATGGCTG GATTTCAGGT GTGGGATGTC 
ACCATGGAAG ATCTTCTTGC TGGAGGCACC ACCCTGGACT CTTTCAGGGG ACTTGTATTT 
GTTGGGGGAT TTAGCTACGC TGATGTGTTA GGGTCTGCCA AAGGTTGGGC CGCCTCGGTG 
AAGTTCAATG CCAGCGTGCG CGAGCAGTTC GAGAACTTCC GCCGTAGGGC AGATACTTTC 
AGCCTTGGGG TTTGCAATGG GTGCCAGCTG ATGGCTCTTC TGGGCTGGGT AGGACCCGAT 
AACCCAACAG ACACAGGTGA TCTCCCCACC CAAGGGGTTC TGCTCAGCCA CAATCTCTCC 
GGCCGGTTTG AATCTCGATT TGTCACCCTG AAGATCCAAC AGAGTCCTTC CATTTTGCTA 
CGAGGGATGG CCGGGTCCAC GCTCGGAGTG TGGGTGGCCC ATGGCGAAGG ATACATGAGG 
TTCCGATCTC CGAAAGTGCA GGACTATGTG ACGTCCAATC ACTTGGCGCC GCTCTGTTAC 
GTGGATGATG GAGGCAAGCC GACGGAAGAA TACCCAATGA ATCCAAATGG CTCTCCGTTG 
GGAATTGCTG GTCTTTGCTC TGCAGATGGG CGGCACTTGG CCATGATGCC CCACCCAGAG 
CGCTGTGTCA TGAAGTGGCA GTGGCCTTGG ATGCCGGAAA GCTGGCGCCA CTCATTGGAT 
GTCTCGCCGT GGATGCGTCT CTTCCAGAAT GGGTACCGCT GGTGCCAGGA GAACCCAAGC 
AGGGATAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012825646.1
CDS	129..4100
Translation	MVVLHFYRSLQNSSETLRLPGAENIQSLQRELCYNVNWTGPAAPSSQETDALRWLFSCPFDPQSISDTSSLHSEPSDLLVEIGPRLNFSTATSTNAVSICRSVGLTNVDRIECSKRYLIKFQTRPQGSEEQELLASLYDRMTECVYPEPVRSFEVSVRPEKVYEVDILGQGRAALEKANTELGLAFDSWDLDYYSSLFQRVGRNPSSVECFDLAQSNSEHSRHWFFKGKLKVDGQEKEMSLFDMIMKTQDTSNPNNVIKFCDNSSAIQGREVASLIPTDPSCAGPYHLCRSTRHLIFTAETHNFPTGVAPFSGATTGTGGRIRDVQSTGRGAHVIAGTAGYCFGNLHIPGYSLPWEDLAYQYPVQFARPLEVAIEASNGASDYGNKFGEPVLAGFARSFGLRLPSGERREWVKPIMFSGGIGSMEDVHRTKEAPEPGMHVVKVGGPVYRIGVGGGAASSIQVQGDNASELDFGAVQRGDAEMEQKMNRAVRACVERGGKNPICSIHDQGAGGNGNVLKELSEPQGAVIYTKSFQLGDPTLSVLEIWGAEYQESNALLLRPDDAEFLRSVCRRERSPVDFVGKITGDGRIVLINGSDTDPAPDSTDRNAVPVDLQLEWVLGKMPRKEFVLNRVAPNLQPLVLPSGLTVGQALDRVLRLPSVASKRYLTNKVDRSVTGLVAQQQCVGPLHTPLADVAVVSLSYSDIVGGATAIGEQPIKSLLNPAAGARLAVAEALTNLLFAQVTDLKDVKCSGNWMWAAKLPGEGALLYDACAAMCDVMAQLGVAIDGGKDSLSMAARVGTETVKAPGSLVISVYAVCPDITTTVTPDLKNPGQKGVLLYLPLCPGQHRLGGSALAQCYSQLGETPPDLDDPQTLISCFNVTQQLLRDRVLSAGHDVSDGGLVTCILEMAFAGNCGLDIEISSSCTNVLELLFAEEPGLVLEVAEQSVELVMERYRAAGVECVRIGCTKERGPASMVRIRANGQEVVNEKLGSLRAVWEETSFQLERLQANPSCVSQEEAGLRVREGPSYHLTFNPSEIPLVPPSVGGNQPRVAVVREEGSNGDREMAASLLMAGFQVWDVTMEDLLAGGTTLDSFRGLVFVGGFSYADVLGSAKGWAASVKFNASVREQFENFRRRADTFSLGVCNGCQLMALLGWVGPDNPTDTGDLPTQGVLLSHNLSGRFESRFVTLKIQQSPSILLRGMAGSTLGVWVAHGEGYMRFRSPKVQDYVTSNHLAPLCYVDDGGKPTEEYPMNPNGSPLGIAGLCSADGRHLAMMPHPERCVMKWQWPWMPESWRHSLDVSPWMRLFQNGYRWCQENPSRDN

Target ORF information:

RefSeq Version	XM_012970192.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphoribosylformylglycinamidine synthase (pfas), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970192.2

ORF Insert Sequence:

ATGGTGGTCC TGCACTTCTA TCGCTCCTTG CAGAATTCAT CAGAGACCCT GCGGCTGCCC 
GGTGCGGAGA ATATACAGAG CCTGCAGAGG GAGCTGTGCT ACAATGTCAA TTGGACAGGT 
CCCGCGGCCC CATCCAGCCA GGAGACTGAC GCCCTTCGAT GGCTGTTTAG TTGCCCCTTT 
GACCCTCAGA GTATCTCTGA CACCTCATCC CTTCACTCGG AGCCTTCTGA CCTGCTGGTA 
GAGATCGGCC CAAGACTGAA TTTCTCCACC GCGACTTCCA CCAATGCTGT GTCCATCTGC 
CGCTCTGTTG GCCTGACCAA CGTGGACAGG ATCGAGTGCT CCAAGAGGTA TCTCATCAAG 
TTCCAGACTA GGCCCCAGGG CAGTGAGGAG CAAGAGCTGC TAGCCTCTCT GTACGACCGT 
ATGACGGAAT GCGTTTACCC GGAGCCGGTG AGGAGTTTCG AGGTGTCCGT TAGACCGGAG 
AAAGTGTACG AGGTGGATAT TTTGGGGCAA GGCAGGGCAG CACTGGAAAA GGCCAACACT 
GAGCTAGGTT TGGCCTTCGA CTCCTGGGAT CTTGATTACT ATAGCAGCCT TTTCCAGCGC 
GTGGGTCGGA ACCCCAGCAG CGTGGAGTGC TTTGATCTTG CACAGTCCAA CAGCGAGCAC 
AGCCGCCACT GGTTCTTTAA GGGCAAATTA AAGGTCGACG GGCAGGAGAA GGAGATGTCT 
CTGTTCGACA TGATCATGAA GACTCAGGAC ACCAGTAATC CCAACAACGT CATCAAGTTC 
TGTGACAACA GCAGTGCGAT CCAGGGAAGA GAAGTGGCAT CTCTAATCCC AACAGACCCT 
TCATGTGCTG GGCCCTATCA TCTCTGTCGC TCCACACGGC ACCTGATTTT CACTGCAGAG 
ACCCACAACT TCCCTACTGG CGTGGCCCCA TTCAGCGGCG CTACGACAGG AACAGGAGGG 
CGCATTCGGG ACGTGCAGAG CACCGGCAGG GGAGCACATG TCATTGCTGG GACGGCTGGG 
TACTGCTTTG GGAACCTGCA CATCCCAGGA TACTCTCTTC CCTGGGAGGA CCTCGCATAT 
CAGTACCCAG TGCAGTTTGC CAGGCCTCTG GAGGTGGCTA TTGAAGCCAG TAACGGAGCC 
TCTGATTATG GAAACAAGTT TGGGGAGCCT GTTTTGGCCG GATTTGCCCG TTCCTTTGGC 
CTGCGGTTGC CTTCGGGAGA AAGGAGGGAG TGGGTGAAAC CCATTATGTT CAGTGGTGGG 
ATCGGATCCA TGGAGGATGT TCACCGTACT AAGGAGGCCC CAGAGCCTGG CATGCACGTG 
GTTAAAGTGG GAGGTCCTGT CTATAGGATT GGGGTTGGCG GTGGAGCTGC CTCTTCTATT 
CAGGTCCAGG GGGATAACGC CAGCGAGCTG GATTTTGGGG CAGTGCAACG TGGAGATGCA 
GAGATGGAGC AGAAGATGAA TCGGGCCGTA CGGGCCTGCG TGGAGCGAGG AGGCAAGAAC 
CCCATCTGCA GCATTCACGA TCAGGGAGCC GGGGGCAACG GGAACGTCCT GAAGGAGCTC 
AGTGAGCCCC AAGGAGCAGT CATTTACACT AAAAGCTTCC AGCTGGGGGA CCCCACCCTG 
AGTGTTCTGG AGATATGGGG AGCAGAATAT CAGGAGTCCA ACGCTTTGCT CCTGCGCCCC 
GACGATGCCG AATTCCTGCG CTCTGTGTGC CGGCGGGAGA GGTCTCCTGT GGATTTTGTG 
GGCAAGATCA CCGGGGATGG CAGGATTGTG TTGATAAATG GCAGTGACAC AGATCCCGCC 
CCTGACTCCA CTGACCGCAA TGCTGTTCCC GTTGACCTCC AGCTGGAATG GGTCTTAGGA 
AAAATGCCTC GCAAGGAGTT TGTTCTAAAC CGCGTGGCAC CAAACTTGCA GCCGCTGGTG 
CTCCCTAGTG GCCTGACAGT CGGGCAGGCG CTTGATCGCG TGCTGAGGTT GCCGTCCGTA 
GCGAGCAAAC GCTACCTGAC CAACAAGGTG GACCGCTCCG TCACAGGCCT GGTGGCCCAG 
CAGCAGTGCG TGGGGCCTCT TCACACTCCA TTGGCCGACG TGGCTGTGGT GTCTCTGTCT 
TATTCGGACA TTGTAGGAGG AGCTACAGCA ATAGGAGAAC AGCCAATCAA AAGCCTACTG 
AATCCTGCAG CTGGGGCAAG ACTGGCGGTG GCCGAGGCAC TAACCAATCT CCTCTTTGCT 
CAAGTGACGG ACCTAAAGGA TGTGAAATGC AGCGGGAACT GGATGTGGGC CGCCAAGCTG 
CCCGGAGAAG GCGCCTTGCT TTACGACGCC TGTGCAGCTA TGTGTGATGT CATGGCGCAG 
CTCGGCGTTG CCATCGATGG AGGAAAAGAT TCTCTCAGCA TGGCCGCCAG AGTGGGCACA 
GAAACAGTTA AAGCTCCAGG TTCGCTAGTG ATCTCGGTGT ATGCAGTCTG CCCCGACATA 
ACTACCACCG TGACCCCCGA TCTTAAGAAC CCAGGGCAGA AAGGTGTTTT ACTGTATTTG 
CCCCTCTGCC CTGGTCAGCA CCGGCTTGGG GGCAGCGCCC TGGCTCAGTG CTACTCACAG 
CTGGGAGAGA CCCCCCCAGA CCTCGATGAC CCTCAGACTT TAATCTCCTG CTTCAACGTC 
ACCCAACAGC TGCTGAGAGA CCGCGTCCTT AGCGCCGGCC ATGACGTCAG TGATGGAGGC 
CTAGTCACGT GTATCCTGGA AATGGCTTTT GCTGGAAACT GTGGCCTGGA TATTGAGATT 
TCCTCCTCCT GCACCAATGT GCTGGAACTG CTGTTTGCCG AGGAACCGGG CCTGGTTCTA 
GAGGTTGCAG AACAAAGTGT AGAACTGGTC ATGGAACGTT ACAGAGCAGC AGGAGTGGAA 
TGCGTGAGGA TCGGATGCAC CAAGGAACGT GGCCCAGCGT CTATGGTCCG AATCCGTGCC 
AATGGGCAGG AGGTGGTCAA CGAGAAGTTG GGTTCCCTCA GAGCCGTATG GGAGGAGACC 
AGTTTCCAAC TTGAACGCCT TCAGGCCAAT CCAAGCTGTG TATCGCAGGA GGAGGCTGGG 
CTGAGAGTGA GGGAGGGGCC AAGTTATCAC CTGACTTTTA ACCCGTCTGA AATACCTTTG 
GTTCCTCCAT CAGTCGGTGG CAACCAACCG CGTGTTGCTG TTGTGCGTGA AGAGGGTAGC 
AACGGAGACC GGGAAATGGC GGCATCTCTC CTAATGGCTG GATTTCAGGT GTGGGATGTC 
ACCATGGAAG ATCTTCTTGC TGGAGGCACC ACCCTGGACT CTTTCAGGGG ACTTGTATTT 
GTTGGGGGAT TTAGCTACGC TGATGTGTTA GGGTCTGCCA AAGGTTGGGC CGCCTCGGTG 
AAGTTCAATG CCAGCGTGCG CGAGCAGTTC GAGAACTTCC GCCGTAGGGC AGATACTTTC 
AGCCTTGGGG TTTGCAATGG GTGCCAGCTG ATGGCTCTTC TGGGCTGGGT AGGACCCGAT 
AACCCAACAG ACACAGGTGA TCTCCCCACC CAAGGGGTTC TGCTCAGCCA CAATCTCTCC 
GGCCGGTTTG AATCTCGATT TGTCACCCTG AAGATCCAAC AGAGTCCTTC CATTTTGCTA 
CGAGGGATGG CCGGGTCCAC GCTCGGAGTG TGGGTGGCCC ATGGCGAAGG ATACATGAGG 
TTCCGATCTC CGAAAGTGCA GGACTATGTG ACGTCCAATC ACTTGGCGCC GCTCTGTTAC 
GTGGATGATG GAGGCAAGCC GACGGAAGAA TACCCAATGA ATCCAAATGG CTCTCCGTTG 
GGAATTGCTG GTCTTTGCTC TGCAGATGGG CGGCACTTGG CCATGATGCC CCACCCAGAG 
CGCTGTGTCA TGAAGTGGCA GTGGCCTTGG ATGCCGGAAA GCTGGCGCCA CTCATTGGAT 
GTCTCGCCGT GGATGCGTCT CTTCCAGAAT GGGTACCGCT GGTGCCAGGA GAACCCAAGC 
AGGGATAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pfas ( XM_012970190.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012825644.2 Xenopus tropicalis phosphoribosylformylglycinamidine synthase (pfas), transcript variant X1, mRNA.

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CloneID	OXa65930
Clone ID Related Accession (Same CDS sequence)	XM_012970190.3
Accession Version	XM_012970190.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3975bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	phosphoribosylformylglycinamidine synthase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970190.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGTCC TGCACTTCTA TCGCTCCTTG CAGAATTCAT CAGAGACCCT GCGGCTGCCC 
GGTGCGGAGA ATATACAGAG CCTGCAGAGG GAGCTGTGCT ACAATGTCAA TTGGACAGGT 
CCCGCGGCCC CATCCAGCCA GGAGACTGAC GCCCTTCGAT GGCTGTTTAG TTGCCCCTTT 
GACCCTCAGA GTATCTCTGA CACCTCATCC CTTCACTCGG AGCCTTCTGA CCTGCTGGTA 
GAGATCGGCC CAAGACTGAA TTTCTCCACC GCGACTTCCA CCAATGCTGT GTCCATCTGC 
CGCTCTGTTG GCCTGACCAA CGTGGACAGG ATCGAGTGCT CCAAGAGGTA TCTCATCAAG 
TTCCAGACTA GGCCCCAGGG CAGTGAGGAG CAAGAGCTGC TAGCCTCTCT GTACGACCGT 
ATGACGGAAT GCGTTTACCC GGAGCCGGTG AGGAGTTTCG AGGTGTCCGT TAGACCGGAG 
AAAGTGTACG AGGTGGATAT TTTGGGGCAA GGCAGGGCAG CACTGGAAAA GGCCAACACT 
GAGCTAGGTT TGGCCTTCGA CTCCTGGGAT CTTGATTACT ATAGCAGCCT TTTCCAGCGC 
GTGGGTCGGA ACCCCAGCAG CGTGGAGTGC TTTGATCTTG CACAGTCCAA CAGCGAGCAC 
AGCCGCCACT GGTTCTTTAA GGGCAAATTA AAGGTCGACG GGCAGGAGAA GGAGATGTCT 
CTGTTCGACA TGATCATGAA GACTCAGGAC ACCAGTAATC CCAACAACGT CATCAAGTTC 
TGTGACAACA GCAGTGCGAT CCAGGGAAGA GAAGTGGCAT CTCTAATCCC AACAGACCCT 
TCATGTGCTG GGCCCTATCA TCTCTGTCGC TCCACACGGC ACCTGATTTT CACTGCAGAG 
ACCCACAACT TCCCTACTGG CGTGGCCCCA TTCAGCGGCG CTACGACAGG AACAGGAGGG 
CGCATTCGGG ACGTGCAGAG CACCGGCAGG GGAGCACATG TCATTGCTGG GACGGCTGGG 
TACTGCTTTG GGAACCTGCA CATCCCAGGA TACTCTCTTC CCTGGGAGGA CCCCGCATAT 
CAGTACCCAG TGCAGTTTGC CAGGCCTCTG GAGGTGGCTA TTGAAGCCAG TAACGGAGCC 
TCTGATTATG GAAACAAGTT TGGGGAGCCT GTTTTGGCCG GATTTGCCCG TTCCTTTGGA 
GTGCGGTTGC CTTCGGGAGA AAGGAGGGAG TGGGTGAAAC CCATTATGTT CAGTGGTGGG 
ATCGGATCCA TGGAGGATGT TCACCGTACT AAGGAGGCCC CAGAGCCTGG CATGCACGTG 
GTTAAAGTGG GAGGTCCTGT CTATAGGATT GGGGTTGGCG GTGGAGCTGC CTCTTCTATT 
CAGGTCCAGG GGGATAACGC CAGCGAGCTG GATTTTGGGG CAGTGCAACG TGGAGATGCA 
GAGATGGAGC AGAAGATGAA TCGGGCCGTA CGGGCCTGCG TGGAGCGAGG AGGCAAGAAC 
CCCATCTGCA GCATTCACGA TCAGGGAGCC GGGGGCAACG GGAACGTCCT GAAGGAGCTC 
AGTGAGCCCC AAGGAGCAGT CATTTACACT AAAAGCTTCC AGCTGGGGGA CCCCACCCTG 
AGTGTTCTGG AGATATGGGG AGCAGAATAT CAGGAGTCCA ACGCTTTGCT CCTGCGCCCC 
GACGATGCCG AATTCCTGCG CTCTGTGTGC CGGCGGGAGA GGTCTCCTGT GGATTTTGTG 
GGCAAGATCA CCGGGGATGG CAGGATTGTG TTGATAAATG GCAGTGACAC AGATCCCGCC 
CCTGACTCCA CTGACCGCAA TGCTGTTCCC GTTGACCTCC AGCTGGAATG GGTCTTAGGA 
AAAATGCCTC GCAAGGAGTT TGTTCTAAAC CGCGTGGCAC CAAACTTGCA GCCGCTGGTG 
CTCCCTAGTG GCCTGACAGT CGGGCAGGCG CTTGATCGCG TGCTGAGGTT GCCGTCCGTA 
GCGAGCAAAC GCTACCTGAC CAACAAGGTG GACCGCTCCG TCACAGGCCT GGTGGCCCAG 
CAGCAGTGCG TGGGGCCTCT TCACACTCCA TTGGCCGACG TGGCTGTGGT GTCTCTGTCT 
TATTCGGACA TTGTAGGAGG AGCTACAGCA ATAGGAGAAC AGCCAATCAA AAGCCTACTG 
AATCCTGCAG CTGGGGCAAG ACTGGCGGTG GCCGAGGCAC TAACCAATCT CCTCTTTGCT 
CAAGTGACGG ACCTAAAGGA TGTGAAATGC AGCGGGAACT GGATGTGGGC CGCCAAGCTG 
CCCGGAGAAG GCGCCTTGCT TTACGACGCC TGTGCAGCTA TGTGTGATGT CATGGCGCAG 
CTCGGCGTTG CCATCGATGG AGGAAAAGAT TCTCTCAGCA TGGCCGCCAG AGTGGGCACA 
GAAACAGTTA AAGCTCCAGG TTCGCTAGTG ATCTCGGTGT ATGCAGTCTG CCCCGACATA 
ACTACCACCG TGACCCCCGA TCTTAAGAAC CCAGGGCAGA AAGGTGTTTT ACTGTATTTG 
CCCCTCTGCC CTGGTCAGCA CCGGCTTGGG GGCAGCGCCC TGGCTCAGTG CTACTCACAG 
CTGGGAGAGA CCCCCCCAGA CCTCGATGAC CCTCAGACTT TAATCTCCTG CTTCAACGTC 
ACCCAACAGC TGCTGAGAGA CCGCGTCCTT AGCGCCGGCC ATGACGTCAG TGATGGAGGC 
CTAGTCACCT GTATCCTGGA AATGGCTTTT GCTGGAAACT GTGGCCTGGA TATTGAGATT 
TCCTCCTCCT GCACCAATGT GCTGGAACTG CTGTTTGCCG AGGAACCGGG CCTGGTTCTA 
GAGGTTGCAG AACAAAGTGT AGAACTGGTC ATGGAACGTT ACAGAGCAGC AGGAGTGGAA 
TGCGTGAGGA TCGGATGCAC CAAGGAACGT GGCCCAGCGT CTATGGTCCG AATCCGTGCC 
AATGGGCAGG AGGTGGTCAA CGAGAAGTTG GGTTCCCTCA GAGCCGTATG GGAGGAGACC 
AGTTTCCAAC TTGAACGCCT TCAGGCCAAT CCAAGCTGTG TATCGCAGGA GGAGGCTGGG 
CTGAGAGTGA GGGAGGGGCC AAGTTATCAC CTGACTTTTA ACCCGTCTGA AATACCTTTG 
GTTCCTCCAT CAGTCGGTGG CAACCAACCG CGTGTTGCTG TTGTGCGTGA AGAGGGTAGC 
AACGGAGACC GGGAAATGGC GGCATCTCTC CTAATGGCTG GATTTCAGGT GTGGGATGTC 
ACCATGGAAG ATCTTCTTGC TGGAGGCACC ACCCTGGACT CTTTCAGGGG ACTTGTATTT 
GTTGGGGGAT TTAGCTACGC TGATGTGTTA GGGTCTGCCA AAGGTTGGGC CGCCTCGGTG 
AAGTTCAATG CCAGCGTGCG CGAGCAGTTC GAGAACTTCC GCCGTAGGGC AGATACTTTC 
AGCCTTGGGG TTTGCAATGG GTGCCAGCTG ATGGCTCTTC TGGGCTGGGT AGGACCCGAT 
AACCCAACAG ACACAGTAGG TGATCTCCCC ACCCAAGGGG TTCTGCTCAG CCACAATCTC 
TCCGGCCGGT TTGAATCTCG ATTTGTCACC CTGAAGATCC AACAGAGTCC TTCCATTTTG 
CTACGAGGGA TGGCCGGGTC CACGCTCGGA GTGTGGGTGG CCCATGGCGA AGGATACATG 
AGGTTCCGAT CTCCGAAAGT GCAGGACTAT GTGACGTCCA ATCACTTGGC GCCGCTCTGT 
TACGTGGATG ATGGAGGCAA GCCGACGGAA GAATACCCAA TGAATCCAAA TGGCTCTCCG 
TTGGGAATTG CTGGTCTTTG CTCTGCAGAT GGGCGGCACT TGGCCATGAT GCCCCACCCA 
GAGCGCTGTG TCATGAAGTG GCAGTGGCCT TGGATGCCGG AAAGCTGGCG CCACTCATTG 
GATGTCTCGC CGTGGATGCG TCTCTTCCAG AATGGGTACC GCTGGTGCCA GGAGAACCCA 
AGCAGGGATA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012825644.2
CDS	101..4075
Translation	MVVLHFYRSLQNSSETLRLPGAENIQSLQRELCYNVNWTGPAAPSSQETDALRWLFSCPFDPQSISDTSSLHSEPSDLLVEIGPRLNFSTATSTNAVSICRSVGLTNVDRIECSKRYLIKFQTRPQGSEEQELLASLYDRMTECVYPEPVRSFEVSVRPEKVYEVDILGQGRAALEKANTELGLAFDSWDLDYYSSLFQRVGRNPSSVECFDLAQSNSEHSRHWFFKGKLKVDGQEKEMSLFDMIMKTQDTSNPNNVIKFCDNSSAIQGREVASLIPTDPSCAGPYHLCRSTRHLIFTAETHNFPTGVAPFSGATTGTGGRIRDVQSTGRGAHVIAGTAGYCFGNLHIPGYSLPWEDPAYQYPVQFARPLEVAIEASNGASDYGNKFGEPVLAGFARSFGVRLPSGERREWVKPIMFSGGIGSMEDVHRTKEAPEPGMHVVKVGGPVYRIGVGGGAASSIQVQGDNASELDFGAVQRGDAEMEQKMNRAVRACVERGGKNPICSIHDQGAGGNGNVLKELSEPQGAVIYTKSFQLGDPTLSVLEIWGAEYQESNALLLRPDDAEFLRSVCRRERSPVDFVGKITGDGRIVLINGSDTDPAPDSTDRNAVPVDLQLEWVLGKMPRKEFVLNRVAPNLQPLVLPSGLTVGQALDRVLRLPSVASKRYLTNKVDRSVTGLVAQQQCVGPLHTPLADVAVVSLSYSDIVGGATAIGEQPIKSLLNPAAGARLAVAEALTNLLFAQVTDLKDVKCSGNWMWAAKLPGEGALLYDACAAMCDVMAQLGVAIDGGKDSLSMAARVGTETVKAPGSLVISVYAVCPDITTTVTPDLKNPGQKGVLLYLPLCPGQHRLGGSALAQCYSQLGETPPDLDDPQTLISCFNVTQQLLRDRVLSAGHDVSDGGLVTCILEMAFAGNCGLDIEISSSCTNVLELLFAEEPGLVLEVAEQSVELVMERYRAAGVECVRIGCTKERGPASMVRIRANGQEVVNEKLGSLRAVWEETSFQLERLQANPSCVSQEEAGLRVREGPSYHLTFNPSEIPLVPPSVGGNQPRVAVVREEGSNGDREMAASLLMAGFQVWDVTMEDLLAGGTTLDSFRGLVFVGGFSYADVLGSAKGWAASVKFNASVREQFENFRRRADTFSLGVCNGCQLMALLGWVGPDNPTDTVGDLPTQGVLLSHNLSGRFESRFVTLKIQQSPSILLRGMAGSTLGVWVAHGEGYMRFRSPKVQDYVTSNHLAPLCYVDDGGKPTEEYPMNPNGSPLGIAGLCSADGRHLAMMPHPERCVMKWQWPWMPESWRHSLDVSPWMRLFQNGYRWCQENPSRDN

Target ORF information:

RefSeq Version	XM_012970190.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphoribosylformylglycinamidine synthase (pfas), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970190.3

ORF Insert Sequence:

ATGGTGGTCC TGCACTTCTA TCGCTCCTTG CAGAATTCAT CAGAGACCCT GCGGCTGCCC 
GGTGCGGAGA ATATACAGAG CCTGCAGAGG GAGCTGTGCT ACAATGTCAA TTGGACAGGT 
CCCGCGGCCC CATCCAGCCA GGAGACTGAC GCCCTTCGAT GGCTGTTTAG TTGCCCCTTT 
GACCCTCAGA GTATCTCTGA CACCTCATCC CTTCACTCGG AGCCTTCTGA CCTGCTGGTA 
GAGATCGGCC CAAGACTGAA TTTCTCCACC GCGACTTCCA CCAATGCTGT GTCCATCTGC 
CGCTCTGTTG GCCTGACCAA CGTGGACAGG ATCGAGTGCT CCAAGAGGTA TCTCATCAAG 
TTCCAGACTA GGCCCCAGGG CAGTGAGGAG CAAGAGCTGC TAGCCTCTCT GTACGACCGT 
ATGACGGAAT GCGTTTACCC GGAGCCGGTG AGGAGTTTCG AGGTGTCCGT TAGACCGGAG 
AAAGTGTACG AGGTGGATAT TTTGGGGCAA GGCAGGGCAG CACTGGAAAA GGCCAACACT 
GAGCTAGGTT TGGCCTTCGA CTCCTGGGAT CTTGATTACT ATAGCAGCCT TTTCCAGCGC 
GTGGGTCGGA ACCCCAGCAG CGTGGAGTGC TTTGATCTTG CACAGTCCAA CAGCGAGCAC 
AGCCGCCACT GGTTCTTTAA GGGCAAATTA AAGGTCGACG GGCAGGAGAA GGAGATGTCT 
CTGTTCGACA TGATCATGAA GACTCAGGAC ACCAGTAATC CCAACAACGT CATCAAGTTC 
TGTGACAACA GCAGTGCGAT CCAGGGAAGA GAAGTGGCAT CTCTAATCCC AACAGACCCT 
TCATGTGCTG GGCCCTATCA TCTCTGTCGC TCCACACGGC ACCTGATTTT CACTGCAGAG 
ACCCACAACT TCCCTACTGG CGTGGCCCCA TTCAGCGGCG CTACGACAGG AACAGGAGGG 
CGCATTCGGG ACGTGCAGAG CACCGGCAGG GGAGCACATG TCATTGCTGG GACGGCTGGG 
TACTGCTTTG GGAACCTGCA CATCCCAGGA TACTCTCTTC CCTGGGAGGA CCCCGCATAT 
CAGTACCCAG TGCAGTTTGC CAGGCCTCTG GAGGTGGCTA TTGAAGCCAG TAACGGAGCC 
TCTGATTATG GAAACAAGTT TGGGGAGCCT GTTTTGGCCG GATTTGCCCG TTCCTTTGGA 
GTGCGGTTGC CTTCGGGAGA AAGGAGGGAG TGGGTGAAAC CCATTATGTT CAGTGGTGGG 
ATCGGATCCA TGGAGGATGT TCACCGTACT AAGGAGGCCC CAGAGCCTGG CATGCACGTG 
GTTAAAGTGG GAGGTCCTGT CTATAGGATT GGGGTTGGCG GTGGAGCTGC CTCTTCTATT 
CAGGTCCAGG GGGATAACGC CAGCGAGCTG GATTTTGGGG CAGTGCAACG TGGAGATGCA 
GAGATGGAGC AGAAGATGAA TCGGGCCGTA CGGGCCTGCG TGGAGCGAGG AGGCAAGAAC 
CCCATCTGCA GCATTCACGA TCAGGGAGCC GGGGGCAACG GGAACGTCCT GAAGGAGCTC 
AGTGAGCCCC AAGGAGCAGT CATTTACACT AAAAGCTTCC AGCTGGGGGA CCCCACCCTG 
AGTGTTCTGG AGATATGGGG AGCAGAATAT CAGGAGTCCA ACGCTTTGCT CCTGCGCCCC 
GACGATGCCG AATTCCTGCG CTCTGTGTGC CGGCGGGAGA GGTCTCCTGT GGATTTTGTG 
GGCAAGATCA CCGGGGATGG CAGGATTGTG TTGATAAATG GCAGTGACAC AGATCCCGCC 
CCTGACTCCA CTGACCGCAA TGCTGTTCCC GTTGACCTCC AGCTGGAATG GGTCTTAGGA 
AAAATGCCTC GCAAGGAGTT TGTTCTAAAC CGCGTGGCAC CAAACTTGCA GCCGCTGGTG 
CTCCCTAGTG GCCTGACAGT CGGGCAGGCG CTTGATCGCG TGCTGAGGTT GCCGTCCGTA 
GCGAGCAAAC GCTACCTGAC CAACAAGGTG GACCGCTCCG TCACAGGCCT GGTGGCCCAG 
CAGCAGTGCG TGGGGCCTCT TCACACTCCA TTGGCCGACG TGGCTGTGGT GTCTCTGTCT 
TATTCGGACA TTGTAGGAGG AGCTACAGCA ATAGGAGAAC AGCCAATCAA AAGCCTACTG 
AATCCTGCAG CTGGGGCAAG ACTGGCGGTG GCCGAGGCAC TAACCAATCT CCTCTTTGCT 
CAAGTGACGG ACCTAAAGGA TGTGAAATGC AGCGGGAACT GGATGTGGGC CGCCAAGCTG 
CCCGGAGAAG GCGCCTTGCT TTACGACGCC TGTGCAGCTA TGTGTGATGT CATGGCGCAG 
CTCGGCGTTG CCATCGATGG AGGAAAAGAT TCTCTCAGCA TGGCCGCCAG AGTGGGCACA 
GAAACAGTTA AAGCTCCAGG TTCGCTAGTG ATCTCGGTGT ATGCAGTCTG CCCCGACATA 
ACTACCACCG TGACCCCCGA TCTTAAGAAC CCAGGGCAGA AAGGTGTTTT ACTGTATTTG 
CCCCTCTGCC CTGGTCAGCA CCGGCTTGGG GGCAGCGCCC TGGCTCAGTG CTACTCACAG 
CTGGGAGAGA CCCCCCCAGA CCTCGATGAC CCTCAGACTT TAATCTCCTG CTTCAACGTC 
ACCCAACAGC TGCTGAGAGA CCGCGTCCTT AGCGCCGGCC ATGACGTCAG TGATGGAGGC 
CTAGTCACCT GTATCCTGGA AATGGCTTTT GCTGGAAACT GTGGCCTGGA TATTGAGATT 
TCCTCCTCCT GCACCAATGT GCTGGAACTG CTGTTTGCCG AGGAACCGGG CCTGGTTCTA 
GAGGTTGCAG AACAAAGTGT AGAACTGGTC ATGGAACGTT ACAGAGCAGC AGGAGTGGAA 
TGCGTGAGGA TCGGATGCAC CAAGGAACGT GGCCCAGCGT CTATGGTCCG AATCCGTGCC 
AATGGGCAGG AGGTGGTCAA CGAGAAGTTG GGTTCCCTCA GAGCCGTATG GGAGGAGACC 
AGTTTCCAAC TTGAACGCCT TCAGGCCAAT CCAAGCTGTG TATCGCAGGA GGAGGCTGGG 
CTGAGAGTGA GGGAGGGGCC AAGTTATCAC CTGACTTTTA ACCCGTCTGA AATACCTTTG 
GTTCCTCCAT CAGTCGGTGG CAACCAACCG CGTGTTGCTG TTGTGCGTGA AGAGGGTAGC 
AACGGAGACC GGGAAATGGC GGCATCTCTC CTAATGGCTG GATTTCAGGT GTGGGATGTC 
ACCATGGAAG ATCTTCTTGC TGGAGGCACC ACCCTGGACT CTTTCAGGGG ACTTGTATTT 
GTTGGGGGAT TTAGCTACGC TGATGTGTTA GGGTCTGCCA AAGGTTGGGC CGCCTCGGTG 
AAGTTCAATG CCAGCGTGCG CGAGCAGTTC GAGAACTTCC GCCGTAGGGC AGATACTTTC 
AGCCTTGGGG TTTGCAATGG GTGCCAGCTG ATGGCTCTTC TGGGCTGGGT AGGACCCGAT 
AACCCAACAG ACACAGTAGG TGATCTCCCC ACCCAAGGGG TTCTGCTCAG CCACAATCTC 
TCCGGCCGGT TTGAATCTCG ATTTGTCACC CTGAAGATCC AACAGAGTCC TTCCATTTTG 
CTACGAGGGA TGGCCGGGTC CACGCTCGGA GTGTGGGTGG CCCATGGCGA AGGATACATG 
AGGTTCCGAT CTCCGAAAGT GCAGGACTAT GTGACGTCCA ATCACTTGGC GCCGCTCTGT 
TACGTGGATG ATGGAGGCAA GCCGACGGAA GAATACCCAA TGAATCCAAA TGGCTCTCCG 
TTGGGAATTG CTGGTCTTTG CTCTGCAGAT GGGCGGCACT TGGCCATGAT GCCCCACCCA 
GAGCGCTGTG TCATGAAGTG GCAGTGGCCT TGGATGCCGG AAAGCTGGCG CCACTCATTG 
GATGTCTCGC CGTGGATGCG TCTCTTCCAG AATGGGTACC GCTGGTGCCA GGAGAACCCA 
AGCAGGGATA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pfas ( XM_012970192.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012825646.2 Xenopus tropicalis phosphoribosylformylglycinamidine synthase (pfas), transcript variant X2, mRNA.

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CloneID	OXa65931
Clone ID Related Accession (Same CDS sequence)	XM_012970192.3
Accession Version	XM_012970192.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	phosphoribosylformylglycinamidine synthase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970192.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGTCC TGCACTTCTA TCGCTCCTTG CAGAATTCAT CAGAGACCCT GCGGCTGCCC 
GGTGCGGAGA ATATACAGAG CCTGCAGAGG GAGCTGTGCT ACAATGTCAA TTGGACAGGT 
CCCGCGGCCC CATCCAGCCA GGAGACTGAC GCCCTTCGAT GGCTGTTTAG TTGCCCCTTT 
GACCCTCAGA GTATCTCTGA CACCTCATCC CTTCACTCGG AGCCTTCTGA CCTGCTGGTA 
GAGATCGGCC CAAGACTGAA TTTCTCCACC GCGACTTCCA CCAATGCTGT GTCCATCTGC 
CGCTCTGTTG GCCTGACCAA CGTGGACAGG ATCGAGTGCT CCAAGAGGTA TCTCATCAAG 
TTCCAGACTA GGCCCCAGGG CAGTGAGGAG CAAGAGCTGC TAGCCTCTCT GTACGACCGT 
ATGACGGAAT GCGTTTACCC GGAGCCGGTG AGGAGTTTCG AGGTGTCCGT TAGACCGGAG 
AAAGTGTACG AGGTGGATAT TTTGGGGCAA GGCAGGGCAG CACTGGAAAA GGCCAACACT 
GAGCTAGGTT TGGCCTTCGA CTCCTGGGAT CTTGATTACT ATAGCAGCCT TTTCCAGCGC 
GTGGGTCGGA ACCCCAGCAG CGTGGAGTGC TTTGATCTTG CACAGTCCAA CAGCGAGCAC 
AGCCGCCACT GGTTCTTTAA GGGCAAATTA AAGGTCGACG GGCAGGAGAA GGAGATGTCT 
CTGTTCGACA TGATCATGAA GACTCAGGAC ACCAGTAATC CCAACAACGT CATCAAGTTC 
TGTGACAACA GCAGTGCGAT CCAGGGAAGA GAAGTGGCAT CTCTAATCCC AACAGACCCT 
TCATGTGCTG GGCCCTATCA TCTCTGTCGC TCCACACGGC ACCTGATTTT CACTGCAGAG 
ACCCACAACT TCCCTACTGG CGTGGCCCCA TTCAGCGGCG CTACGACAGG AACAGGAGGG 
CGCATTCGGG ACGTGCAGAG CACCGGCAGG GGAGCACATG TCATTGCTGG GACGGCTGGG 
TACTGCTTTG GGAACCTGCA CATCCCAGGA TACTCTCTTC CCTGGGAGGA CCCCGCATAT 
CAGTACCCAG TGCAGTTTGC CAGGCCTCTG GAGGTGGCTA TTGAAGCCAG TAACGGAGCC 
TCTGATTATG GAAACAAGTT TGGGGAGCCT GTTTTGGCCG GATTTGCCCG TTCCTTTGGA 
GTGCGGTTGC CTTCGGGAGA AAGGAGGGAG TGGGTGAAAC CCATTATGTT CAGTGGTGGG 
ATCGGATCCA TGGAGGATGT TCACCGTACT AAGGAGGCCC CAGAGCCTGG CATGCACGTG 
GTTAAAGTGG GAGGTCCTGT CTATAGGATT GGGGTTGGCG GTGGAGCTGC CTCTTCTATT 
CAGGTCCAGG GGGATAACGC CAGCGAGCTG GATTTTGGGG CAGTGCAACG TGGAGATGCA 
GAGATGGAGC AGAAGATGAA TCGGGCCGTA CGGGCCTGCG TGGAGCGAGG AGGCAAGAAC 
CCCATCTGCA GCATTCACGA TCAGGGAGCC GGGGGCAACG GGAACGTCCT GAAGGAGCTC 
AGTGAGCCCC AAGGAGCAGT CATTTACACT AAAAGCTTCC AGCTGGGGGA CCCCACCCTG 
AGTGTTCTGG AGATATGGGG AGCAGAATAT CAGGAGTCCA ACGCTTTGCT CCTGCGCCCC 
GACGATGCCG AATTCCTGCG CTCTGTGTGC CGGCGGGAGA GGTCTCCTGT GGATTTTGTG 
GGCAAGATCA CCGGGGATGG CAGGATTGTG TTGATAAATG GCAGTGACAC AGATCCCGCC 
CCTGACTCCA CTGACCGCAA TGCTGTTCCC GTTGACCTCC AGCTGGAATG GGTCTTAGGA 
AAAATGCCTC GCAAGGAGTT TGTTCTAAAC CGCGTGGCAC CAAACTTGCA GCCGCTGGTG 
CTCCCTAGTG GCCTGACAGT CGGGCAGGCG CTTGATCGCG TGCTGAGGTT GCCGTCCGTA 
GCGAGCAAAC GCTACCTGAC CAACAAGGTG GACCGCTCCG TCACAGGCCT GGTGGCCCAG 
CAGCAGTGCG TGGGGCCTCT TCACACTCCA TTGGCCGACG TGGCTGTGGT GTCTCTGTCT 
TATTCGGACA TTGTAGGAGG AGCTACAGCA ATAGGAGAAC AGCCAATCAA AAGCCTACTG 
AATCCTGCAG CTGGGGCAAG ACTGGCGGTG GCCGAGGCAC TAACCAATCT CCTCTTTGCT 
CAAGTGACGG ACCTAAAGGA TGTGAAATGC AGCGGGAACT GGATGTGGGC CGCCAAGCTG 
CCCGGAGAAG GCGCCTTGCT TTACGACGCC TGTGCAGCTA TGTGTGATGT CATGGCGCAG 
CTCGGCGTTG CCATCGATGG AGGAAAAGAT TCTCTCAGCA TGGCCGCCAG AGTGGGCACA 
GAAACAGTTA AAGCTCCAGG TTCGCTAGTG ATCTCGGTGT ATGCAGTCTG CCCCGACATA 
ACTACCACCG TGACCCCCGA TCTTAAGAAC CCAGGGCAGA AAGGTGTTTT ACTGTATTTG 
CCCCTCTGCC CTGGTCAGCA CCGGCTTGGG GGCAGCGCCC TGGCTCAGTG CTACTCACAG 
CTGGGAGAGA CCCCCCCAGA CCTCGATGAC CCTCAGACTT TAATCTCCTG CTTCAACGTC 
ACCCAACAGC TGCTGAGAGA CCGCGTCCTT AGCGCCGGCC ATGACGTCAG TGATGGAGGC 
CTAGTCACCT GTATCCTGGA AATGGCTTTT GCTGGAAACT GTGGCCTGGA TATTGAGATT 
TCCTCCTCCT GCACCAATGT GCTGGAACTG CTGTTTGCCG AGGAACCGGG CCTGGTTCTA 
GAGGTTGCAG AACAAAGTGT AGAACTGGTC ATGGAACGTT ACAGAGCAGC AGGAGTGGAA 
TGCGTGAGGA TCGGATGCAC CAAGGAACGT GGCCCAGCGT CTATGGTCCG AATCCGTGCC 
AATGGGCAGG AGGTGGTCAA CGAGAAGTTG GGTTCCCTCA GAGCCGTATG GGAGGAGACC 
AGTTTCCAAC TTGAACGCCT TCAGGCCAAT CCAAGCTGTG TATCGCAGGA GGAGGCTGGG 
CTGAGAGTGA GGGAGGGGCC AAGTTATCAC CTGACTTTTA ACCCGTCTGA AATACCTTTG 
GTTCCTCCAT CAGTCGGTGG CAACCAACCG CGTGTTGCTG TTGTGCGTGA AGAGGGTAGC 
AACGGAGACC GGGAAATGGC GGCATCTCTC CTAATGGCTG GATTTCAGGT GTGGGATGTC 
ACCATGGAAG ATCTTCTTGC TGGAGGCACC ACCCTGGACT CTTTCAGGGG ACTTGTATTT 
GTTGGGGGAT TTAGCTACGC TGATGTGTTA GGGTCTGCCA AAGGTTGGGC CGCCTCGGTG 
AAGTTCAATG CCAGCGTGCG CGAGCAGTTC GAGAACTTCC GCCGTAGGGC AGATACTTTC 
AGCCTTGGGG TTTGCAATGG GTGCCAGCTG ATGGCTCTTC TGGGCTGGGT AGGACCCGAT 
AACCCAACAG ACACAGGTGA TCTCCCCACC CAAGGGGTTC TGCTCAGCCA CAATCTCTCC 
GGCCGGTTTG AATCTCGATT TGTCACCCTG AAGATCCAAC AGAGTCCTTC CATTTTGCTA 
CGAGGGATGG CCGGGTCCAC GCTCGGAGTG TGGGTGGCCC ATGGCGAAGG ATACATGAGG 
TTCCGATCTC CGAAAGTGCA GGACTATGTG ACGTCCAATC ACTTGGCGCC GCTCTGTTAC 
GTGGATGATG GAGGCAAGCC GACGGAAGAA TACCCAATGA ATCCAAATGG CTCTCCGTTG 
GGAATTGCTG GTCTTTGCTC TGCAGATGGG CGGCACTTGG CCATGATGCC CCACCCAGAG 
CGCTGTGTCA TGAAGTGGCA GTGGCCTTGG ATGCCGGAAA GCTGGCGCCA CTCATTGGAT 
GTCTCGCCGT GGATGCGTCT CTTCCAGAAT GGGTACCGCT GGTGCCAGGA GAACCCAAGC 
AGGGATAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012825646.2
CDS	112..4083
Translation	MVVLHFYRSLQNSSETLRLPGAENIQSLQRELCYNVNWTGPAAPSSQETDALRWLFSCPFDPQSISDTSSLHSEPSDLLVEIGPRLNFSTATSTNAVSICRSVGLTNVDRIECSKRYLIKFQTRPQGSEEQELLASLYDRMTECVYPEPVRSFEVSVRPEKVYEVDILGQGRAALEKANTELGLAFDSWDLDYYSSLFQRVGRNPSSVECFDLAQSNSEHSRHWFFKGKLKVDGQEKEMSLFDMIMKTQDTSNPNNVIKFCDNSSAIQGREVASLIPTDPSCAGPYHLCRSTRHLIFTAETHNFPTGVAPFSGATTGTGGRIRDVQSTGRGAHVIAGTAGYCFGNLHIPGYSLPWEDPAYQYPVQFARPLEVAIEASNGASDYGNKFGEPVLAGFARSFGVRLPSGERREWVKPIMFSGGIGSMEDVHRTKEAPEPGMHVVKVGGPVYRIGVGGGAASSIQVQGDNASELDFGAVQRGDAEMEQKMNRAVRACVERGGKNPICSIHDQGAGGNGNVLKELSEPQGAVIYTKSFQLGDPTLSVLEIWGAEYQESNALLLRPDDAEFLRSVCRRERSPVDFVGKITGDGRIVLINGSDTDPAPDSTDRNAVPVDLQLEWVLGKMPRKEFVLNRVAPNLQPLVLPSGLTVGQALDRVLRLPSVASKRYLTNKVDRSVTGLVAQQQCVGPLHTPLADVAVVSLSYSDIVGGATAIGEQPIKSLLNPAAGARLAVAEALTNLLFAQVTDLKDVKCSGNWMWAAKLPGEGALLYDACAAMCDVMAQLGVAIDGGKDSLSMAARVGTETVKAPGSLVISVYAVCPDITTTVTPDLKNPGQKGVLLYLPLCPGQHRLGGSALAQCYSQLGETPPDLDDPQTLISCFNVTQQLLRDRVLSAGHDVSDGGLVTCILEMAFAGNCGLDIEISSSCTNVLELLFAEEPGLVLEVAEQSVELVMERYRAAGVECVRIGCTKERGPASMVRIRANGQEVVNEKLGSLRAVWEETSFQLERLQANPSCVSQEEAGLRVREGPSYHLTFNPSEIPLVPPSVGGNQPRVAVVREEGSNGDREMAASLLMAGFQVWDVTMEDLLAGGTTLDSFRGLVFVGGFSYADVLGSAKGWAASVKFNASVREQFENFRRRADTFSLGVCNGCQLMALLGWVGPDNPTDTGDLPTQGVLLSHNLSGRFESRFVTLKIQQSPSILLRGMAGSTLGVWVAHGEGYMRFRSPKVQDYVTSNHLAPLCYVDDGGKPTEEYPMNPNGSPLGIAGLCSADGRHLAMMPHPERCVMKWQWPWMPESWRHSLDVSPWMRLFQNGYRWCQENPSRDN

Target ORF information:

RefSeq Version	XM_012970192.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphoribosylformylglycinamidine synthase (pfas), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970192.3

ORF Insert Sequence:

ATGGTGGTCC TGCACTTCTA TCGCTCCTTG CAGAATTCAT CAGAGACCCT GCGGCTGCCC 
GGTGCGGAGA ATATACAGAG CCTGCAGAGG GAGCTGTGCT ACAATGTCAA TTGGACAGGT 
CCCGCGGCCC CATCCAGCCA GGAGACTGAC GCCCTTCGAT GGCTGTTTAG TTGCCCCTTT 
GACCCTCAGA GTATCTCTGA CACCTCATCC CTTCACTCGG AGCCTTCTGA CCTGCTGGTA 
GAGATCGGCC CAAGACTGAA TTTCTCCACC GCGACTTCCA CCAATGCTGT GTCCATCTGC 
CGCTCTGTTG GCCTGACCAA CGTGGACAGG ATCGAGTGCT CCAAGAGGTA TCTCATCAAG 
TTCCAGACTA GGCCCCAGGG CAGTGAGGAG CAAGAGCTGC TAGCCTCTCT GTACGACCGT 
ATGACGGAAT GCGTTTACCC GGAGCCGGTG AGGAGTTTCG AGGTGTCCGT TAGACCGGAG 
AAAGTGTACG AGGTGGATAT TTTGGGGCAA GGCAGGGCAG CACTGGAAAA GGCCAACACT 
GAGCTAGGTT TGGCCTTCGA CTCCTGGGAT CTTGATTACT ATAGCAGCCT TTTCCAGCGC 
GTGGGTCGGA ACCCCAGCAG CGTGGAGTGC TTTGATCTTG CACAGTCCAA CAGCGAGCAC 
AGCCGCCACT GGTTCTTTAA GGGCAAATTA AAGGTCGACG GGCAGGAGAA GGAGATGTCT 
CTGTTCGACA TGATCATGAA GACTCAGGAC ACCAGTAATC CCAACAACGT CATCAAGTTC 
TGTGACAACA GCAGTGCGAT CCAGGGAAGA GAAGTGGCAT CTCTAATCCC AACAGACCCT 
TCATGTGCTG GGCCCTATCA TCTCTGTCGC TCCACACGGC ACCTGATTTT CACTGCAGAG 
ACCCACAACT TCCCTACTGG CGTGGCCCCA TTCAGCGGCG CTACGACAGG AACAGGAGGG 
CGCATTCGGG ACGTGCAGAG CACCGGCAGG GGAGCACATG TCATTGCTGG GACGGCTGGG 
TACTGCTTTG GGAACCTGCA CATCCCAGGA TACTCTCTTC CCTGGGAGGA CCCCGCATAT 
CAGTACCCAG TGCAGTTTGC CAGGCCTCTG GAGGTGGCTA TTGAAGCCAG TAACGGAGCC 
TCTGATTATG GAAACAAGTT TGGGGAGCCT GTTTTGGCCG GATTTGCCCG TTCCTTTGGA 
GTGCGGTTGC CTTCGGGAGA AAGGAGGGAG TGGGTGAAAC CCATTATGTT CAGTGGTGGG 
ATCGGATCCA TGGAGGATGT TCACCGTACT AAGGAGGCCC CAGAGCCTGG CATGCACGTG 
GTTAAAGTGG GAGGTCCTGT CTATAGGATT GGGGTTGGCG GTGGAGCTGC CTCTTCTATT 
CAGGTCCAGG GGGATAACGC CAGCGAGCTG GATTTTGGGG CAGTGCAACG TGGAGATGCA 
GAGATGGAGC AGAAGATGAA TCGGGCCGTA CGGGCCTGCG TGGAGCGAGG AGGCAAGAAC 
CCCATCTGCA GCATTCACGA TCAGGGAGCC GGGGGCAACG GGAACGTCCT GAAGGAGCTC 
AGTGAGCCCC AAGGAGCAGT CATTTACACT AAAAGCTTCC AGCTGGGGGA CCCCACCCTG 
AGTGTTCTGG AGATATGGGG AGCAGAATAT CAGGAGTCCA ACGCTTTGCT CCTGCGCCCC 
GACGATGCCG AATTCCTGCG CTCTGTGTGC CGGCGGGAGA GGTCTCCTGT GGATTTTGTG 
GGCAAGATCA CCGGGGATGG CAGGATTGTG TTGATAAATG GCAGTGACAC AGATCCCGCC 
CCTGACTCCA CTGACCGCAA TGCTGTTCCC GTTGACCTCC AGCTGGAATG GGTCTTAGGA 
AAAATGCCTC GCAAGGAGTT TGTTCTAAAC CGCGTGGCAC CAAACTTGCA GCCGCTGGTG 
CTCCCTAGTG GCCTGACAGT CGGGCAGGCG CTTGATCGCG TGCTGAGGTT GCCGTCCGTA 
GCGAGCAAAC GCTACCTGAC CAACAAGGTG GACCGCTCCG TCACAGGCCT GGTGGCCCAG 
CAGCAGTGCG TGGGGCCTCT TCACACTCCA TTGGCCGACG TGGCTGTGGT GTCTCTGTCT 
TATTCGGACA TTGTAGGAGG AGCTACAGCA ATAGGAGAAC AGCCAATCAA AAGCCTACTG 
AATCCTGCAG CTGGGGCAAG ACTGGCGGTG GCCGAGGCAC TAACCAATCT CCTCTTTGCT 
CAAGTGACGG ACCTAAAGGA TGTGAAATGC AGCGGGAACT GGATGTGGGC CGCCAAGCTG 
CCCGGAGAAG GCGCCTTGCT TTACGACGCC TGTGCAGCTA TGTGTGATGT CATGGCGCAG 
CTCGGCGTTG CCATCGATGG AGGAAAAGAT TCTCTCAGCA TGGCCGCCAG AGTGGGCACA 
GAAACAGTTA AAGCTCCAGG TTCGCTAGTG ATCTCGGTGT ATGCAGTCTG CCCCGACATA 
ACTACCACCG TGACCCCCGA TCTTAAGAAC CCAGGGCAGA AAGGTGTTTT ACTGTATTTG 
CCCCTCTGCC CTGGTCAGCA CCGGCTTGGG GGCAGCGCCC TGGCTCAGTG CTACTCACAG 
CTGGGAGAGA CCCCCCCAGA CCTCGATGAC CCTCAGACTT TAATCTCCTG CTTCAACGTC 
ACCCAACAGC TGCTGAGAGA CCGCGTCCTT AGCGCCGGCC ATGACGTCAG TGATGGAGGC 
CTAGTCACCT GTATCCTGGA AATGGCTTTT GCTGGAAACT GTGGCCTGGA TATTGAGATT 
TCCTCCTCCT GCACCAATGT GCTGGAACTG CTGTTTGCCG AGGAACCGGG CCTGGTTCTA 
GAGGTTGCAG AACAAAGTGT AGAACTGGTC ATGGAACGTT ACAGAGCAGC AGGAGTGGAA 
TGCGTGAGGA TCGGATGCAC CAAGGAACGT GGCCCAGCGT CTATGGTCCG AATCCGTGCC 
AATGGGCAGG AGGTGGTCAA CGAGAAGTTG GGTTCCCTCA GAGCCGTATG GGAGGAGACC 
AGTTTCCAAC TTGAACGCCT TCAGGCCAAT CCAAGCTGTG TATCGCAGGA GGAGGCTGGG 
CTGAGAGTGA GGGAGGGGCC AAGTTATCAC CTGACTTTTA ACCCGTCTGA AATACCTTTG 
GTTCCTCCAT CAGTCGGTGG CAACCAACCG CGTGTTGCTG TTGTGCGTGA AGAGGGTAGC 
AACGGAGACC GGGAAATGGC GGCATCTCTC CTAATGGCTG GATTTCAGGT GTGGGATGTC 
ACCATGGAAG ATCTTCTTGC TGGAGGCACC ACCCTGGACT CTTTCAGGGG ACTTGTATTT 
GTTGGGGGAT TTAGCTACGC TGATGTGTTA GGGTCTGCCA AAGGTTGGGC CGCCTCGGTG 
AAGTTCAATG CCAGCGTGCG CGAGCAGTTC GAGAACTTCC GCCGTAGGGC AGATACTTTC 
AGCCTTGGGG TTTGCAATGG GTGCCAGCTG ATGGCTCTTC TGGGCTGGGT AGGACCCGAT 
AACCCAACAG ACACAGGTGA TCTCCCCACC CAAGGGGTTC TGCTCAGCCA CAATCTCTCC 
GGCCGGTTTG AATCTCGATT TGTCACCCTG AAGATCCAAC AGAGTCCTTC CATTTTGCTA 
CGAGGGATGG CCGGGTCCAC GCTCGGAGTG TGGGTGGCCC ATGGCGAAGG ATACATGAGG 
TTCCGATCTC CGAAAGTGCA GGACTATGTG ACGTCCAATC ACTTGGCGCC GCTCTGTTAC 
GTGGATGATG GAGGCAAGCC GACGGAAGAA TACCCAATGA ATCCAAATGG CTCTCCGTTG 
GGAATTGCTG GTCTTTGCTC TGCAGATGGG CGGCACTTGG CCATGATGCC CCACCCAGAG 
CGCTGTGTCA TGAAGTGGCA GTGGCCTTGG ATGCCGGAAA GCTGGCGCCA CTCATTGGAT 
GTCTCGCCGT GGATGCGTCT CTTCCAGAAT GGGTACCGCT GGTGCCAGGA GAACCCAAGC 
AGGGATAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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pfas cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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