upf2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	upf2
Entrez Gene ID	100158573
Full Name	UPF2, regulator of nonsense mediated mRNA decay
Synonyms	hupf2,rent2,smg-3
General protein information	Preferred Names UPF2, regulator of nonsense mediated mRNA decay Names regulator of nonsense transcripts 2 UPF2 regulator of nonsense transcripts homolog
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012958640.2	XP_012814094.1	regulator of nonsense transcripts 2 isoform X1
NM_001128003.1	NP_001121475.1	regulator of nonsense transcripts 2
XM_012958639.2	XP_012814093.1	regulator of nonsense transcripts 2 isoform X1
XM_012958641.3	XP_012814095.2	regulator of nonsense transcripts 2 isoform X2
XM_012958639.3	XP_012814093.2	regulator of nonsense transcripts 2 isoform X1
XM_012958641.2	XP_012814095.1	regulator of nonsense transcripts 2 isoform X2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	UPF2	XP_005214170.1
Homo sapiens (human)	UPF2	NP_542166.1
Rattus norvegicus (Norway rat)	Upf2	NP_001101891.1
Danio rerio (zebrafish)	upf2	XP_697460.6
Drosophila melanogaster (fruit fly)	Upf2	NP_572434.1
Neurospora crassa	NCU05267	XP_961757.2
Eremothecium gossypii	AGOS_AGR364C	NP_987030.2
Saccharomyces cerevisiae (baker's yeast)	NMD2	NP_011944.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000337	XP_310780.5
Magnaporthe oryzae (rice blast fungus)	MGG_06063	XP_003711916.1
Kluyveromyces lactis	KLLA0D13156g	XP_453649.1
Pan troglodytes (chimpanzee)	UPF2	XP_507656.3
Macaca mulatta (Rhesus monkey)	UPF2	XP_002805601.1
Gallus gallus (chicken)	UPF2	XP_004937542.1
Arabidopsis thaliana (thale cress)	AT2G39260	NP_181459.4
Canis lupus familiaris (dog)	UPF2	XP_005617199.1
Mus musculus (house mouse)	Upf2	NP_001074601.1
Schizosaccharomyces pombe (fission yeast)	upf2	NP_593784.1
Xenopus tropicalis (tropical clawed frog)	upf2	NP_001121475.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following upf2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the upf2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa24799	NM_001128003.1 Latest version!	Xenopus tropicalis UPF2, regulator of nonsense mediated mRNA decay (upf2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa24799	XM_012958639.3 Latest version!	Xenopus tropicalis UPF2, regulator of nonsense mediated mRNA decay (upf2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa32206	XM_012958640.2 Latest version!	Xenopus tropicalis UPF2 regulator of nonsense transcripts homolog (upf2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa32206	XM_012958639.2 Latest version!	Xenopus tropicalis UPF2 regulator of nonsense transcripts homolog (upf2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa32207	XM_012958641.2 Latest version!	Xenopus tropicalis UPF2 regulator of nonsense transcripts homolog (upf2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa53297	XM_012958641.3 Latest version!	Xenopus tropicalis UPF2, regulator of nonsense mediated mRNA decay (upf2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

upf2 ( NM_001128003.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001121475.1 Xenopus tropicalis UPF2, regulator of nonsense mediated mRNA decay (upf2), mRNA.

Price & Availability↑

CloneID	OXa24799
Clone ID Related Accession (Same CDS sequence)	NM_001128003.1 , XM_012958639.3
Accession Version	NM_001128003.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576857600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	regulator of nonsense transcripts 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC166247.1. ##Evidence-Data-START## Transcript exon combination :: BC166247.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028290, SAMD00028291 [ECO:0000348] ##Evidence-Data-END##

ATGCCAGCAG AACGCAAGAA ACCAGCAGGT ATGGAAGAAA AGGAATCTCC ATCTAGCCCA 
AGGGACAAAG AATGTGCTGA GAAGAAATCT ACAAGCAGCA AAGATCGGGT TAAGGAAGAA 
CCAAAAGCAA GCACCAAAAG AGATCAATCA AAGGCTTCTG AGGAAAAAAA GAAGCGAACT 
GATGAGGACA AGCGGAAAAA AGAGGAGAAG GAGTGCAAAA GAAAGGAGGA TGATCAAGTC 
AAAGCAGATG ATGAGCAGCA AAGTTCAGAG GGAAAAAATC AAGAAGATGA AATAAAAAAG 
AAGCAGGAAG AAGAGGAAGC TCATCAGAGA GAAGCTGCAG CATTACACAT CAAAGAGAAG 
GATGAAGCAA ATCAGTTACA CCAAGAAGCA TGGGAACGGC ACCACACTAG GAAGGAGCTT 
CGTAGCAAAA ATCAAAATGC CCCTGAAAAT CGACCTGAGG AGAGTTTCTT TAGCAGGCTG 
GACTCTAGTT TGAAGAAGAA CACTGCTTTT GTGAAGAAAC TCAAAACCAT TACTGAACAG 
CAAAGAGATT CATTGTCCCA TGACTTCAAC AGCCTTAACC TCAGCAAGTA CATAGCAGAG 
GCAGCAGCCT CCATTGTAGA GGCCAAGTTA AAAATATCTG ATGTTAACTG TGCTGTGCAT 
CTGTGTTCTC TTTTCCACCA GCGATATGCT GACTTTGCAC AGTCTCTCCT TCAGGTATGG 
AAGAAGCATT TTGAAGCCCG CAAAGAGGAA AAGACTCCTA ATATTACTAA GCTGAGAACA 
GACTTGCGCT TTATTGCAGA GCTAACCATC ATTGGCATCT TTACTGATAA AGAAGGCCTC 
TCTCTAATTT ATGAGCAACT TAAAAACATA ATTAATGCTG ATCGAGAATC CCACACACAT 
GTTTCAGTAG TAATCAGTTT TTGCCGGCAC TGTGGAGATG ATATTGCAGG CTTAGTGCCC 
AGGAAAGTGA AGTGTGCAGC TGAAAAATAC AATCTTAATT TTCCTCCTAG TGAGATTATC 
AGCCCAGAGA AACAACAGCC TTTCCAGAAT TTGCTGAAAG AGTATTTCAC ATCTCTGACG 
AAACACTTGA AGAGGGACCA CAGAGAATTA CAAAATATAG AGCGCCAGAA CAGGCGCATT 
CTTCACACTA AAGGGGAGCT AAGTGAAGAC AGGCACAAGC AATATGAGGA GTTTGCAACA 
TCTTATCAGA AGCTGTTAGC TAGCTGTCAA TCACTTGCAG ATCTGCTAGA TGAAAATATG 
CCAGAACTTC CTCAGGACAA ACCTGCTCCG GAGGAACATG GGCCTGGTAT TGATATCTTC 
ACACCTGGAA AACCTGGAGA TTATGACTTA GAAGGAGGCA TTTGGGAAGA CGAGGATGCC 
CGTAACTTTT ATGAGAATCT GATTGATTTG AAAGCTTTTG TCCCAGCCAT TTTATTTAAG 
GATAATGAGA AAAATTCTCA GAGTAAAGAA ACTGGCAAAG ACAGTTTCAA AGATAGTAAA 
GAGAAAGATG TGTCCACAAC TGAAGAGCTA GAGCTGGAGT TAGAGACATT GGATATTAAT 
GATGAAGGCA TGGAACTGGA AGGTGCCGAA GATGCAGAAG ACCTAACAAA AAAACTTTTG 
GATGAACAAG AGCAAGAAGA TGAAGAGGCA AGCACAGGCT CCCATTTAAA ACTCATTGTT 
GATGCTTTTA TTCAGCAGCT GCCAAACTGT GTTAATCGTG ATCTAATAGA TAAGGCTGCA 
ATGGATTTTT GCATGAACAT GAATACAAAA GCTAATAGAA GGAAATTGGT CAGAGCACTA 
TTCATTGTTC CCAGACAAAG ACTGGATCTT CTTCCATTCT ATGCCAGATT AGTGGCCACC 
TTGCATCCTT GCATGTCTGA TGTTGCAGAA GACCTTTGTT CCATGTTAAA GGGAGACTTT 
CGTTTCCACG TTAGAAAGAA GGATCAAATC AACATTGAAA CAAAGAACAA GACTGTGAGA 
TTTATTGGAG AACTTGCCAA ATTTAAAATG TTCAGCAAAA CAGATACGCT GCATTGTTTA 
AAGATGCTGT TGTCAGACTT TTCGCATCAC CACATAGAAA TGGCATGCAC TCTACTGGAA 
ACTTGTGGCA GATTTCTTTT TCGATCTCCA GATTCTCACT TGCGCACCAG TGTCCTGCTG 
GAGCAAATGA TGCGCAAGAA GCAAGCAATG CACTTAGATG CACGCTATGT TACAATGGTT 
GAAAATGCCT ACTATTACTG TAACCCACCT CCAGCTGAAA AAACTGTGAA AAAGAAGCGA 
CCTCCACTAC AAGAATACAT CAGAAAGCTT CTTTATAAAG ACCTTTCCAA GGTCACCACT 
GAAAAGGTTT TGAGGCAGAT GAGGAAGCTG TCTTGGCAGG ATCCTGAAGT GAAGGACTAT 
GTAATTTGCT GCATGATCAA CATCTGGAAT GTTAAATACA ACAGCATTCA TTGTGTAGCC 
AATCTTTTGG CAGGACTGGT ACTTTACCAA GAGGATGTTG GAATACATGT TGTAGATGGA 
GTGTTAGAAG ATATACGTCT TGGGATGGAG GTTAACCAGC CAAAATTTAA TCAGCGGAGG 
ATAAGCAGTG CAAAGTTCCT TGGGGAGCTT TACAACTATA GAATGGTGGA ATCTGCAGTT 
ATATTTCGCA CACTTTATTC ATTTATTTCC TTTGGTGTAA ATTCTGATGG AAGCCCCAGC 
CCACTTGATC CTCCTGAACA CTTGTTCCGC ATTCGATTAG TTTGCACTAT CCTTGACACT 
TGTGGCCAGT ATTTTGACCG TGGATCAAGC AAGAGAAAGC TTGATTGTTT TCTTGTTTAT 
TTTCAGCGCT ACGTTTGGTG GAAGAAAAGT CTTGATGTGT GGACTAAAGA CCACCCATTT 
CCAATAGATA TTGATTATAT GATAAGTGAT ACCTTGGAGC TTCTGAGGCC AAAAATGAAG 
CTCTGTGCCT CACTGAATGA ATCTGTCCGC CAAGTGCAGG ATTTGGAGCG TGAGTTTCTG 
ACAAAGCTAG GTCTCATGAA TGATAAGGAA TCCAAACATT CCATGACAGA AGGAGATCAC 
CTAGTGTATG AAGATGATGA TGAGGAAGAA GAGGCTGGGG CAGAGACAGA GGAGCAGTCT 
GGCAATGAAA GTGAAATGAA TGAACAAGAA GAAGATGCTT CTGAAAATGA AGAGGATGAA 
AGAGAGGAGG AAGAAGAGGA GAATACAGAT TATATGACTG ACTCAAACAA AGAAAATGAA 
ACAGATGAAG AAAACACTGA GGTGATGATT AAAGGAGGTG GAATTAAACA TGTACCCTGT 
GCTGAAGATG AAGACTTTAT TCAAGCCCTT GACAAGATGA TGCTGGAAAA TCTTCAGCAA 
CGTAGTGGAG AGTCAGTGAA GGTGCATCAG TTAGATGTTG CTATTCCCCT GCATCTTAAG 
AGCCAGCTTA AAAAGGGACC AGCTATGAGC ACTGGAGAAG TAGACCCGGA ATCTGGCGAC 
ACTATGCCAT TTGTTATGCT TACAAGAAGA GGTAACAAAC AGCAGTTCAA GATCCTTAAT 
GTGCCTATGT CCTCCCAACT GGCTGCAAAT CACTGGAGTC AGCAGCAAGC TGAACAAGAA 
GAGAGGATGA GGATGAAGAA ACTCACATTA GATATCAATG AGCGTCAGGA ACAAGAAGAT 
TACCAAGAAA TGTTGCAGTC ATTGGCTCAG CGCCCAGCAC CTGCCAACAC AAACAGGGAG 
CGCAGGCCTC GATACCAGCA TCCAAAGGGA GCACCTAACG CAGACCTCAT CTTCAAAACT 
GGGGGCAGGA GACGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001121475.1
CDS	148..3945
Translation	MPAERKKPAGMEEKESPSSPRDKECAEKKSTSSKDRVKEEPKASTKRDQSKASEEKKKRTDEDKRKKEEKECKRKEDDQVKADDEQQSSEGKNQEDEIKKKQEEEEAHQREAAALHIKEKDEANQLHQEAWERHHTRKELRSKNQNAPENRPEESFFSRLDSSLKKNTAFVKKLKTITEQQRDSLSHDFNSLNLSKYIAEAAASIVEAKLKISDVNCAVHLCSLFHQRYADFAQSLLQVWKKHFEARKEEKTPNITKLRTDLRFIAELTIIGIFTDKEGLSLIYEQLKNIINADRESHTHVSVVISFCRHCGDDIAGLVPRKVKCAAEKYNLNFPPSEIISPEKQQPFQNLLKEYFTSLTKHLKRDHRELQNIERQNRRILHTKGELSEDRHKQYEEFATSYQKLLASCQSLADLLDENMPELPQDKPAPEEHGPGIDIFTPGKPGDYDLEGGIWEDEDARNFYENLIDLKAFVPAILFKDNEKNSQSKETGKDSFKDSKEKDVSTTEELELELETLDINDEGMELEGAEDAEDLTKKLLDEQEQEDEEASTGSHLKLIVDAFIQQLPNCVNRDLIDKAAMDFCMNMNTKANRRKLVRALFIVPRQRLDLLPFYARLVATLHPCMSDVAEDLCSMLKGDFRFHVRKKDQINIETKNKTVRFIGELAKFKMFSKTDTLHCLKMLLSDFSHHHIEMACTLLETCGRFLFRSPDSHLRTSVLLEQMMRKKQAMHLDARYVTMVENAYYYCNPPPAEKTVKKKRPPLQEYIRKLLYKDLSKVTTEKVLRQMRKLSWQDPEVKDYVICCMINIWNVKYNSIHCVANLLAGLVLYQEDVGIHVVDGVLEDIRLGMEVNQPKFNQRRISSAKFLGELYNYRMVESAVIFRTLYSFISFGVNSDGSPSPLDPPEHLFRIRLVCTILDTCGQYFDRGSSKRKLDCFLVYFQRYVWWKKSLDVWTKDHPFPIDIDYMISDTLELLRPKMKLCASLNESVRQVQDLEREFLTKLGLMNDKESKHSMTEGDHLVYEDDDEEEEAGAETEEQSGNESEMNEQEEDASENEEDEREEEEEENTDYMTDSNKENETDEENTEVMIKGGGIKHVPCAEDEDFIQALDKMMLENLQQRSGESVKVHQLDVAIPLHLKSQLKKGPAMSTGEVDPESGDTMPFVMLTRRGNKQQFKILNVPMSSQLAANHWSQQQAEQEERMRMKKLTLDINERQEQEDYQEMLQSLAQRPAPANTNRERRPRYQHPKGAPNADLIFKTGGRRR

Target ORF information:

RefSeq Version	NM_001128003.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis UPF2, regulator of nonsense mediated mRNA decay (upf2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001128003.1

ORF Insert Sequence:

ATGCCAGCAG AACGCAAGAA ACCAGCAGGT ATGGAAGAAA AGGAATCTCC ATCTAGCCCA 
AGGGACAAAG AATGTGCTGA GAAGAAATCT ACAAGCAGCA AAGATCGGGT TAAGGAAGAA 
CCAAAAGCAA GCACCAAAAG AGATCAATCA AAGGCTTCTG AGGAAAAAAA GAAGCGAACT 
GATGAGGACA AGCGGAAAAA AGAGGAGAAG GAGTGCAAAA GAAAGGAGGA TGATCAAGTC 
AAAGCAGATG ATGAGCAGCA AAGTTCAGAG GGAAAAAATC AAGAAGATGA AATAAAAAAG 
AAGCAGGAAG AAGAGGAAGC TCATCAGAGA GAAGCTGCAG CATTACACAT CAAAGAGAAG 
GATGAAGCAA ATCAGTTACA CCAAGAAGCA TGGGAACGGC ACCACACTAG GAAGGAGCTT 
CGTAGCAAAA ATCAAAATGC CCCTGAAAAT CGACCTGAGG AGAGTTTCTT TAGCAGGCTG 
GACTCTAGTT TGAAGAAGAA CACTGCTTTT GTGAAGAAAC TCAAAACCAT TACTGAACAG 
CAAAGAGATT CATTGTCCCA TGACTTCAAC AGCCTTAACC TCAGCAAGTA CATAGCAGAG 
GCAGCAGCCT CCATTGTAGA GGCCAAGTTA AAAATATCTG ATGTTAACTG TGCTGTGCAT 
CTGTGTTCTC TTTTCCACCA GCGATATGCT GACTTTGCAC AGTCTCTCCT TCAGGTATGG 
AAGAAGCATT TTGAAGCCCG CAAAGAGGAA AAGACTCCTA ATATTACTAA GCTGAGAACA 
GACTTGCGCT TTATTGCAGA GCTAACCATC ATTGGCATCT TTACTGATAA AGAAGGCCTC 
TCTCTAATTT ATGAGCAACT TAAAAACATA ATTAATGCTG ATCGAGAATC CCACACACAT 
GTTTCAGTAG TAATCAGTTT TTGCCGGCAC TGTGGAGATG ATATTGCAGG CTTAGTGCCC 
AGGAAAGTGA AGTGTGCAGC TGAAAAATAC AATCTTAATT TTCCTCCTAG TGAGATTATC 
AGCCCAGAGA AACAACAGCC TTTCCAGAAT TTGCTGAAAG AGTATTTCAC ATCTCTGACG 
AAACACTTGA AGAGGGACCA CAGAGAATTA CAAAATATAG AGCGCCAGAA CAGGCGCATT 
CTTCACACTA AAGGGGAGCT AAGTGAAGAC AGGCACAAGC AATATGAGGA GTTTGCAACA 
TCTTATCAGA AGCTGTTAGC TAGCTGTCAA TCACTTGCAG ATCTGCTAGA TGAAAATATG 
CCAGAACTTC CTCAGGACAA ACCTGCTCCG GAGGAACATG GGCCTGGTAT TGATATCTTC 
ACACCTGGAA AACCTGGAGA TTATGACTTA GAAGGAGGCA TTTGGGAAGA CGAGGATGCC 
CGTAACTTTT ATGAGAATCT GATTGATTTG AAAGCTTTTG TCCCAGCCAT TTTATTTAAG 
GATAATGAGA AAAATTCTCA GAGTAAAGAA ACTGGCAAAG ACAGTTTCAA AGATAGTAAA 
GAGAAAGATG TGTCCACAAC TGAAGAGCTA GAGCTGGAGT TAGAGACATT GGATATTAAT 
GATGAAGGCA TGGAACTGGA AGGTGCCGAA GATGCAGAAG ACCTAACAAA AAAACTTTTG 
GATGAACAAG AGCAAGAAGA TGAAGAGGCA AGCACAGGCT CCCATTTAAA ACTCATTGTT 
GATGCTTTTA TTCAGCAGCT GCCAAACTGT GTTAATCGTG ATCTAATAGA TAAGGCTGCA 
ATGGATTTTT GCATGAACAT GAATACAAAA GCTAATAGAA GGAAATTGGT CAGAGCACTA 
TTCATTGTTC CCAGACAAAG ACTGGATCTT CTTCCATTCT ATGCCAGATT AGTGGCCACC 
TTGCATCCTT GCATGTCTGA TGTTGCAGAA GACCTTTGTT CCATGTTAAA GGGAGACTTT 
CGTTTCCACG TTAGAAAGAA GGATCAAATC AACATTGAAA CAAAGAACAA GACTGTGAGA 
TTTATTGGAG AACTTGCCAA ATTTAAAATG TTCAGCAAAA CAGATACGCT GCATTGTTTA 
AAGATGCTGT TGTCAGACTT TTCGCATCAC CACATAGAAA TGGCATGCAC TCTACTGGAA 
ACTTGTGGCA GATTTCTTTT TCGATCTCCA GATTCTCACT TGCGCACCAG TGTCCTGCTG 
GAGCAAATGA TGCGCAAGAA GCAAGCAATG CACTTAGATG CACGCTATGT TACAATGGTT 
GAAAATGCCT ACTATTACTG TAACCCACCT CCAGCTGAAA AAACTGTGAA AAAGAAGCGA 
CCTCCACTAC AAGAATACAT CAGAAAGCTT CTTTATAAAG ACCTTTCCAA GGTCACCACT 
GAAAAGGTTT TGAGGCAGAT GAGGAAGCTG TCTTGGCAGG ATCCTGAAGT GAAGGACTAT 
GTAATTTGCT GCATGATCAA CATCTGGAAT GTTAAATACA ACAGCATTCA TTGTGTAGCC 
AATCTTTTGG CAGGACTGGT ACTTTACCAA GAGGATGTTG GAATACATGT TGTAGATGGA 
GTGTTAGAAG ATATACGTCT TGGGATGGAG GTTAACCAGC CAAAATTTAA TCAGCGGAGG 
ATAAGCAGTG CAAAGTTCCT TGGGGAGCTT TACAACTATA GAATGGTGGA ATCTGCAGTT 
ATATTTCGCA CACTTTATTC ATTTATTTCC TTTGGTGTAA ATTCTGATGG AAGCCCCAGC 
CCACTTGATC CTCCTGAACA CTTGTTCCGC ATTCGATTAG TTTGCACTAT CCTTGACACT 
TGTGGCCAGT ATTTTGACCG TGGATCAAGC AAGAGAAAGC TTGATTGTTT TCTTGTTTAT 
TTTCAGCGCT ACGTTTGGTG GAAGAAAAGT CTTGATGTGT GGACTAAAGA CCACCCATTT 
CCAATAGATA TTGATTATAT GATAAGTGAT ACCTTGGAGC TTCTGAGGCC AAAAATGAAG 
CTCTGTGCCT CACTGAATGA ATCTGTCCGC CAAGTGCAGG ATTTGGAGCG TGAGTTTCTG 
ACAAAGCTAG GTCTCATGAA TGATAAGGAA TCCAAACATT CCATGACAGA AGGAGATCAC 
CTAGTGTATG AAGATGATGA TGAGGAAGAA GAGGCTGGGG CAGAGACAGA GGAGCAGTCT 
GGCAATGAAA GTGAAATGAA TGAACAAGAA GAAGATGCTT CTGAAAATGA AGAGGATGAA 
AGAGAGGAGG AAGAAGAGGA GAATACAGAT TATATGACTG ACTCAAACAA AGAAAATGAA 
ACAGATGAAG AAAACACTGA GGTGATGATT AAAGGAGGTG GAATTAAACA TGTACCCTGT 
GCTGAAGATG AAGACTTTAT TCAAGCCCTT GACAAGATGA TGCTGGAAAA TCTTCAGCAA 
CGTAGTGGAG AGTCAGTGAA GGTGCATCAG TTAGATGTTG CTATTCCCCT GCATCTTAAG 
AGCCAGCTTA AAAAGGGACC AGCTATGAGC ACTGGAGAAG TAGACCCGGA ATCTGGCGAC 
ACTATGCCAT TTGTTATGCT TACAAGAAGA GGTAACAAAC AGCAGTTCAA GATCCTTAAT 
GTGCCTATGT CCTCCCAACT GGCTGCAAAT CACTGGAGTC AGCAGCAAGC TGAACAAGAA 
GAGAGGATGA GGATGAAGAA ACTCACATTA GATATCAATG AGCGTCAGGA ACAAGAAGAT 
TACCAAGAAA TGTTGCAGTC ATTGGCTCAG CGCCCAGCAC CTGCCAACAC AAACAGGGAG 
CGCAGGCCTC GATACCAGCA TCCAAAGGGA GCACCTAACG CAGACCTCAT CTTCAAAACT 
GGGGGCAGGA GACGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

upf2 ( XM_012958639.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814093.2 Xenopus tropicalis UPF2, regulator of nonsense mediated mRNA decay (upf2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa24799
Clone ID Related Accession (Same CDS sequence)	NM_001128003.1 , XM_012958639.3
Accession Version	XM_012958639.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	regulator of nonsense transcripts 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012958639.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGCAG AACGCAAGAA ACCAGCAGGT ATGGAAGAAA AGGAATCTCC ATCTAGCCCA 
AGGGACAAAG AATGTGCTGA GAAGAAATCT ACAAGCAGCA AAGATCGGGT TAAGGAAGAA 
CCAAAAGCAA GCACCAAAAG AGATCAATCA AAGGCTTCTG AGGAAAAAAA GAAGCGAACT 
GATGAGGACA AGCGGAAAAA AGAGGAGAAG GAGTGCAAAA GAAAGGAGGA TGATCAAGTC 
AAAGCAGATG ATGAGCAGCA AAGTTCAGAG GGAAAAAATC AAGAAGATGA AATAAAAAAG 
AAGCAGGAAG AAGAGGAAGC TCATCAGAGA GAAGCTGCAG CATTACACAT CAAAGAGAAG 
GATGAAGCAA ATCAGTTACA CCAAGAAGCA TGGGAACGGC ACCACACTAG GAAGGAGCTT 
CGTAGCAAAA ATCAAAATGC CCCTGAAAAT CGACCTGAGG AGAGTTTCTT TAGCAGGCTG 
GACTCTAGTT TGAAGAAGAA CACTGCTTTT GTGAAGAAAC TCAAAACCAT TACTGAACAG 
CAAAGAGATT CATTGTCCCA TGACTTCAAC AGCCTTAACC TCAGCAAGTA CATAGCAGAG 
GCAGCAGCCT CCATTGTAGA GGCCAAGTTA AAAATATCTG ATGTTAACTG TGCTGTGCAT 
CTGTGTTCTC TTTTCCACCA GCGATATGCT GACTTTGCAC AGTCTCTCCT TCAGGTATGG 
AAGAAGCATT TTGAAGCCCG CAAAGAGGAA AAGACTCCTA ATATTACTAA GCTGAGAACA 
GACTTGCGCT TTATTGCAGA GCTAACCATC ATTGGCATCT TTACTGATAA AGAAGGCCTC 
TCTCTAATTT ATGAGCAACT TAAAAACATA ATTAATGCTG ATCGAGAATC CCACACACAT 
GTTTCAGTAG TAATCAGTTT TTGCCGGCAC TGTGGAGATG ATATTGCAGG CTTAGTGCCC 
AGGAAAGTGA AGTGTGCAGC TGAAAAATAC AATCTTAATT TTCCTCCTAG TGAGATTATC 
AGCCCAGAGA AACAACAGCC TTTCCAGAAT TTGCTGAAAG AGTATTTCAC ATCTCTGACG 
AAACACTTGA AGAGGGACCA CAGAGAATTA CAAAATATAG AGCGCCAGAA CAGGCGCATT 
CTTCACACTA AAGGGGAGCT AAGTGAAGAC AGGCACAAGC AATATGAGGA GTTTGCAACA 
TCTTATCAGA AGCTGTTAGC TAGCTGTCAA TCACTTGCAG ATCTGCTAGA TGAAAATATG 
CCAGAACTTC CTCAGGACAA ACCTGCTCCG GAGGAACATG GGCCTGGTAT TGATATCTTC 
ACACCTGGAA AACCTGGAGA TTATGACTTA GAAGGAGGCA TTTGGGAAGA CGAGGATGCC 
CGTAACTTTT ATGAGAATCT GATTGATTTG AAAGCTTTTG TCCCAGCCAT TTTATTTAAG 
GATAATGAGA AAAATTCTCA GAGTAAAGAA ACTGGCAAAG ACAGTTTCAA AGATAGTAAA 
GAGAAAGATG TGTCCACAAC TGAAGAGCTA GAGCTGGAGT TAGAGACATT GGATATTAAT 
GATGAAGGCA TGGAACTGGA AGGTGCCGAA GATGCAGAAG ACCTAACAAA AAAACTTTTG 
GATGAACAAG AGCAAGAAGA TGAAGAGGCA AGCACAGGCT CCCATTTAAA ACTCATTGTT 
GATGCTTTTA TTCAGCAGCT GCCAAACTGT GTTAATCGTG ATCTAATAGA TAAGGCTGCA 
ATGGATTTTT GCATGAACAT GAATACAAAA GCTAATAGAA GGAAATTGGT CAGAGCACTA 
TTCATTGTTC CCAGACAAAG ACTGGATCTT CTTCCATTCT ATGCCAGATT AGTGGCCACC 
TTGCATCCTT GCATGTCTGA TGTTGCAGAA GACCTTTGTT CCATGTTAAA GGGAGACTTT 
CGTTTCCACG TTAGAAAGAA GGATCAAATC AACATTGAAA CAAAGAACAA GACTGTGAGA 
TTTATTGGAG AACTTGCCAA ATTTAAAATG TTCAGCAAAA CAGATACGCT GCATTGTTTA 
AAGATGCTGT TGTCAGACTT TTCGCATCAC CACATAGAAA TGGCATGCAC TCTACTGGAA 
ACTTGTGGCA GATTTCTTTT TCGATCTCCA GATTCTCACT TGCGCACCAG TGTCCTGCTG 
GAGCAAATGA TGCGCAAGAA GCAAGCAATG CACTTAGATG CACGCTATGT TACAATGGTT 
GAAAATGCCT ACTATTACTG TAACCCACCT CCAGCTGAAA AAACTGTGAA AAAGAAGCGA 
CCTCCACTAC AAGAATACAT CAGAAAGCTT CTTTATAAAG ACCTTTCCAA GGTCACCACT 
GAAAAGGTTT TGAGGCAGAT GAGGAAGCTG TCTTGGCAGG ATCCTGAAGT GAAGGACTAT 
GTAATTTGCT GCATGATCAA CATCTGGAAT GTTAAATACA ACAGCATTCA TTGTGTAGCC 
AATCTTTTGG CAGGACTGGT ACTTTACCAA GAGGATGTTG GAATACATGT TGTAGATGGA 
GTGTTAGAAG ATATACGTCT TGGGATGGAG GTTAACCAGC CAAAATTTAA TCAGCGGAGG 
ATAAGCAGTG CAAAGTTCCT TGGGGAGCTT TACAACTATA GAATGGTGGA ATCTGCAGTT 
ATATTTCGCA CACTTTATTC ATTTATTTCC TTTGGTGTAA ATTCTGATGG AAGCCCCAGC 
CCACTTGATC CTCCTGAACA CTTGTTCCGC ATTCGATTAG TTTGCACTAT CCTTGACACT 
TGTGGCCAGT ATTTTGACCG TGGATCAAGC AAGAGAAAGC TTGATTGTTT TCTTGTTTAT 
TTTCAGCGCT ACGTTTGGTG GAAGAAAAGT CTTGATGTGT GGACTAAAGA CCACCCATTT 
CCAATAGATA TTGATTATAT GATAAGTGAT ACCTTGGAGC TTCTGAGGCC AAAAATGAAG 
CTCTGTGCCT CACTGAATGA ATCTGTCCGC CAAGTGCAGG ATTTGGAGCG TGAGTTTCTG 
ACAAAGCTAG GTCTCATGAA TGATAAGGAA TCCAAACATT CCATGACAGA AGGAGATCAC 
CTAGTGTATG AAGATGATGA TGAGGAAGAA GAGGCTGGGG CAGAGACAGA GGAGCAGTCT 
GGCAATGAAA GTGAAATGAA TGAACAAGAA GAAGATGCTT CTGAAAATGA AGAGGATGAA 
AGAGAGGAGG AAGAAGAGGA GAATACAGAT TATATGACTG ACTCAAACAA AGAAAATGAA 
ACAGATGAAG AAAACACTGA GGTGATGATT AAAGGAGGTG GAATTAAACA TGTACCCTGT 
GCTGAAGATG AAGACTTTAT TCAAGCCCTT GACAAGATGA TGCTGGAAAA TCTTCAGCAA 
CGTAGTGGAG AGTCAGTGAA GGTGCATCAG TTAGATGTTG CTATTCCCCT GCATCTTAAG 
AGCCAGCTTA AAAAGGGACC AGCTATGAGC ACTGGAGAAG TAGACCCGGA ATCTGGCGAC 
ACTATGCCAT TTGTTATGCT TACAAGAAGA GGTAACAAAC AGCAGTTCAA GATCCTTAAT 
GTGCCTATGT CCTCCCAACT GGCTGCAAAT CACTGGAGTC AGCAGCAAGC TGAACAAGAA 
GAGAGGATGA GGATGAAGAA ACTCACATTA GATATCAATG AGCGTCAGGA ACAAGAAGAT 
TACCAAGAAA TGTTGCAGTC ATTGGCTCAG CGCCCAGCAC CTGCCAACAC AAACAGGGAG 
CGCAGGCCTC GATACCAGCA TCCAAAGGGA GCACCTAACG CAGACCTCAT CTTCAAAACT 
GGGGGCAGGA GACGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814093.2
CDS	385..4182
Translation	MPAERKKPAGMEEKESPSSPRDKECAEKKSTSSKDRVKEEPKASTKRDQSKASEEKKKRTDEDKRKKEEKECKRKEDDQVKADDEQQSSEGKNQEDEIKKKQEEEEAHQREAAALHIKEKDEANQLHQEAWERHHTRKELRSKNQNAPENRPEESFFSRLDSSLKKNTAFVKKLKTITEQQRDSLSHDFNSLNLSKYIAEAAASIVEAKLKISDVNCAVHLCSLFHQRYADFAQSLLQVWKKHFEARKEEKTPNITKLRTDLRFIAELTIIGIFTDKEGLSLIYEQLKNIINADRESHTHVSVVISFCRHCGDDIAGLVPRKVKCAAEKYNLNFPPSEIISPEKQQPFQNLLKEYFTSLTKHLKRDHRELQNIERQNRRILHTKGELSEDRHKQYEEFATSYQKLLASCQSLADLLDENMPELPQDKPAPEEHGPGIDIFTPGKPGDYDLEGGIWEDEDARNFYENLIDLKAFVPAILFKDNEKNSQSKETGKDSFKDSKEKDVSTTEELELELETLDINDEGMELEGAEDAEDLTKKLLDEQEQEDEEASTGSHLKLIVDAFIQQLPNCVNRDLIDKAAMDFCMNMNTKANRRKLVRALFIVPRQRLDLLPFYARLVATLHPCMSDVAEDLCSMLKGDFRFHVRKKDQINIETKNKTVRFIGELAKFKMFSKTDTLHCLKMLLSDFSHHHIEMACTLLETCGRFLFRSPDSHLRTSVLLEQMMRKKQAMHLDARYVTMVENAYYYCNPPPAEKTVKKKRPPLQEYIRKLLYKDLSKVTTEKVLRQMRKLSWQDPEVKDYVICCMINIWNVKYNSIHCVANLLAGLVLYQEDVGIHVVDGVLEDIRLGMEVNQPKFNQRRISSAKFLGELYNYRMVESAVIFRTLYSFISFGVNSDGSPSPLDPPEHLFRIRLVCTILDTCGQYFDRGSSKRKLDCFLVYFQRYVWWKKSLDVWTKDHPFPIDIDYMISDTLELLRPKMKLCASLNESVRQVQDLEREFLTKLGLMNDKESKHSMTEGDHLVYEDDDEEEEAGAETEEQSGNESEMNEQEEDASENEEDEREEEEEENTDYMTDSNKENETDEENTEVMIKGGGIKHVPCAEDEDFIQALDKMMLENLQQRSGESVKVHQLDVAIPLHLKSQLKKGPAMSTGEVDPESGDTMPFVMLTRRGNKQQFKILNVPMSSQLAANHWSQQQAEQEERMRMKKLTLDINERQEQEDYQEMLQSLAQRPAPANTNRERRPRYQHPKGAPNADLIFKTGGRRR

Target ORF information:

RefSeq Version	XM_012958639.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis UPF2, regulator of nonsense mediated mRNA decay (upf2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012958639.3

ORF Insert Sequence:

ATGCCAGCAG AACGCAAGAA ACCAGCAGGT ATGGAAGAAA AGGAATCTCC ATCTAGCCCA 
AGGGACAAAG AATGTGCTGA GAAGAAATCT ACAAGCAGCA AAGATCGGGT TAAGGAAGAA 
CCAAAAGCAA GCACCAAAAG AGATCAATCA AAGGCTTCTG AGGAAAAAAA GAAGCGAACT 
GATGAGGACA AGCGGAAAAA AGAGGAGAAG GAGTGCAAAA GAAAGGAGGA TGATCAAGTC 
AAAGCAGATG ATGAGCAGCA AAGTTCAGAG GGAAAAAATC AAGAAGATGA AATAAAAAAG 
AAGCAGGAAG AAGAGGAAGC TCATCAGAGA GAAGCTGCAG CATTACACAT CAAAGAGAAG 
GATGAAGCAA ATCAGTTACA CCAAGAAGCA TGGGAACGGC ACCACACTAG GAAGGAGCTT 
CGTAGCAAAA ATCAAAATGC CCCTGAAAAT CGACCTGAGG AGAGTTTCTT TAGCAGGCTG 
GACTCTAGTT TGAAGAAGAA CACTGCTTTT GTGAAGAAAC TCAAAACCAT TACTGAACAG 
CAAAGAGATT CATTGTCCCA TGACTTCAAC AGCCTTAACC TCAGCAAGTA CATAGCAGAG 
GCAGCAGCCT CCATTGTAGA GGCCAAGTTA AAAATATCTG ATGTTAACTG TGCTGTGCAT 
CTGTGTTCTC TTTTCCACCA GCGATATGCT GACTTTGCAC AGTCTCTCCT TCAGGTATGG 
AAGAAGCATT TTGAAGCCCG CAAAGAGGAA AAGACTCCTA ATATTACTAA GCTGAGAACA 
GACTTGCGCT TTATTGCAGA GCTAACCATC ATTGGCATCT TTACTGATAA AGAAGGCCTC 
TCTCTAATTT ATGAGCAACT TAAAAACATA ATTAATGCTG ATCGAGAATC CCACACACAT 
GTTTCAGTAG TAATCAGTTT TTGCCGGCAC TGTGGAGATG ATATTGCAGG CTTAGTGCCC 
AGGAAAGTGA AGTGTGCAGC TGAAAAATAC AATCTTAATT TTCCTCCTAG TGAGATTATC 
AGCCCAGAGA AACAACAGCC TTTCCAGAAT TTGCTGAAAG AGTATTTCAC ATCTCTGACG 
AAACACTTGA AGAGGGACCA CAGAGAATTA CAAAATATAG AGCGCCAGAA CAGGCGCATT 
CTTCACACTA AAGGGGAGCT AAGTGAAGAC AGGCACAAGC AATATGAGGA GTTTGCAACA 
TCTTATCAGA AGCTGTTAGC TAGCTGTCAA TCACTTGCAG ATCTGCTAGA TGAAAATATG 
CCAGAACTTC CTCAGGACAA ACCTGCTCCG GAGGAACATG GGCCTGGTAT TGATATCTTC 
ACACCTGGAA AACCTGGAGA TTATGACTTA GAAGGAGGCA TTTGGGAAGA CGAGGATGCC 
CGTAACTTTT ATGAGAATCT GATTGATTTG AAAGCTTTTG TCCCAGCCAT TTTATTTAAG 
GATAATGAGA AAAATTCTCA GAGTAAAGAA ACTGGCAAAG ACAGTTTCAA AGATAGTAAA 
GAGAAAGATG TGTCCACAAC TGAAGAGCTA GAGCTGGAGT TAGAGACATT GGATATTAAT 
GATGAAGGCA TGGAACTGGA AGGTGCCGAA GATGCAGAAG ACCTAACAAA AAAACTTTTG 
GATGAACAAG AGCAAGAAGA TGAAGAGGCA AGCACAGGCT CCCATTTAAA ACTCATTGTT 
GATGCTTTTA TTCAGCAGCT GCCAAACTGT GTTAATCGTG ATCTAATAGA TAAGGCTGCA 
ATGGATTTTT GCATGAACAT GAATACAAAA GCTAATAGAA GGAAATTGGT CAGAGCACTA 
TTCATTGTTC CCAGACAAAG ACTGGATCTT CTTCCATTCT ATGCCAGATT AGTGGCCACC 
TTGCATCCTT GCATGTCTGA TGTTGCAGAA GACCTTTGTT CCATGTTAAA GGGAGACTTT 
CGTTTCCACG TTAGAAAGAA GGATCAAATC AACATTGAAA CAAAGAACAA GACTGTGAGA 
TTTATTGGAG AACTTGCCAA ATTTAAAATG TTCAGCAAAA CAGATACGCT GCATTGTTTA 
AAGATGCTGT TGTCAGACTT TTCGCATCAC CACATAGAAA TGGCATGCAC TCTACTGGAA 
ACTTGTGGCA GATTTCTTTT TCGATCTCCA GATTCTCACT TGCGCACCAG TGTCCTGCTG 
GAGCAAATGA TGCGCAAGAA GCAAGCAATG CACTTAGATG CACGCTATGT TACAATGGTT 
GAAAATGCCT ACTATTACTG TAACCCACCT CCAGCTGAAA AAACTGTGAA AAAGAAGCGA 
CCTCCACTAC AAGAATACAT CAGAAAGCTT CTTTATAAAG ACCTTTCCAA GGTCACCACT 
GAAAAGGTTT TGAGGCAGAT GAGGAAGCTG TCTTGGCAGG ATCCTGAAGT GAAGGACTAT 
GTAATTTGCT GCATGATCAA CATCTGGAAT GTTAAATACA ACAGCATTCA TTGTGTAGCC 
AATCTTTTGG CAGGACTGGT ACTTTACCAA GAGGATGTTG GAATACATGT TGTAGATGGA 
GTGTTAGAAG ATATACGTCT TGGGATGGAG GTTAACCAGC CAAAATTTAA TCAGCGGAGG 
ATAAGCAGTG CAAAGTTCCT TGGGGAGCTT TACAACTATA GAATGGTGGA ATCTGCAGTT 
ATATTTCGCA CACTTTATTC ATTTATTTCC TTTGGTGTAA ATTCTGATGG AAGCCCCAGC 
CCACTTGATC CTCCTGAACA CTTGTTCCGC ATTCGATTAG TTTGCACTAT CCTTGACACT 
TGTGGCCAGT ATTTTGACCG TGGATCAAGC AAGAGAAAGC TTGATTGTTT TCTTGTTTAT 
TTTCAGCGCT ACGTTTGGTG GAAGAAAAGT CTTGATGTGT GGACTAAAGA CCACCCATTT 
CCAATAGATA TTGATTATAT GATAAGTGAT ACCTTGGAGC TTCTGAGGCC AAAAATGAAG 
CTCTGTGCCT CACTGAATGA ATCTGTCCGC CAAGTGCAGG ATTTGGAGCG TGAGTTTCTG 
ACAAAGCTAG GTCTCATGAA TGATAAGGAA TCCAAACATT CCATGACAGA AGGAGATCAC 
CTAGTGTATG AAGATGATGA TGAGGAAGAA GAGGCTGGGG CAGAGACAGA GGAGCAGTCT 
GGCAATGAAA GTGAAATGAA TGAACAAGAA GAAGATGCTT CTGAAAATGA AGAGGATGAA 
AGAGAGGAGG AAGAAGAGGA GAATACAGAT TATATGACTG ACTCAAACAA AGAAAATGAA 
ACAGATGAAG AAAACACTGA GGTGATGATT AAAGGAGGTG GAATTAAACA TGTACCCTGT 
GCTGAAGATG AAGACTTTAT TCAAGCCCTT GACAAGATGA TGCTGGAAAA TCTTCAGCAA 
CGTAGTGGAG AGTCAGTGAA GGTGCATCAG TTAGATGTTG CTATTCCCCT GCATCTTAAG 
AGCCAGCTTA AAAAGGGACC AGCTATGAGC ACTGGAGAAG TAGACCCGGA ATCTGGCGAC 
ACTATGCCAT TTGTTATGCT TACAAGAAGA GGTAACAAAC AGCAGTTCAA GATCCTTAAT 
GTGCCTATGT CCTCCCAACT GGCTGCAAAT CACTGGAGTC AGCAGCAAGC TGAACAAGAA 
GAGAGGATGA GGATGAAGAA ACTCACATTA GATATCAATG AGCGTCAGGA ACAAGAAGAT 
TACCAAGAAA TGTTGCAGTC ATTGGCTCAG CGCCCAGCAC CTGCCAACAC AAACAGGGAG 
CGCAGGCCTC GATACCAGCA TCCAAAGGGA GCACCTAACG CAGACCTCAT CTTCAAAACT 
GGGGGCAGGA GACGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

upf2 ( XM_012958640.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814094.1 Xenopus tropicalis UPF2 regulator of nonsense transcripts homolog (upf2), transcript variant X2, mRNA.

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CloneID	OXa32206
Clone ID Related Accession (Same CDS sequence)	XM_012958640.2 , XM_012958639.2
Accession Version	XM_012958640.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	regulator of nonsense transcripts 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012958640.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGCAG AACGCAAGAA ACCAGCAGGT ATGGAAGAAA AGGAATCTCC ATCTAGCCCA 
AGGGACAAAG AATGTGCTGA GAAGAAATCT ACAAGCAGCA AAGATCGGGT TAAGGAAGAA 
CCAAAAGCAA GCACCAAAAG AGATCAATCA AAGGCTTCTG AGGAAAAAAA GAAGCGAACT 
GATGAGGACA AGCGGAAAAA AGAGGAGAAG GAGTGCAAAA GAAAGGAGGA TGATCAAGTC 
AAAGCAGATG ATGAGCAGCA AAGTGCAGAG GGAAAAAATC AAGAAGATGA AATAAAAAAG 
AAGCAGGAAG AAGAGGAAGC TCATCAGAGA GAAGCTGCAG CATTGCAAAT CAAAGAGAAG 
GATGAAGCAA ATCAGTTACA CCAAGAAGCA TGGGAACGGC ACCACACTAG GAAGGAGCTT 
CGTAGCAAAA ATCAAAATGC CCCTGAAAAT CGACCTGAGG AGAGTTTCTT TAGCAGGCTG 
GACTCTAGTT TGAAGAAGAA CACTGCTTTT GTGAAGAAAC TCAAAACCAT TACTGAACAG 
CAAAGAGATT CATTGTCCCA TGACTTCAAC AGCCTTAACC TCAGCAAGTA CATAGCAGAG 
GCAGCAGCCT CCATTGTAGA GGCCAAGTTA AAAATATCTG ATGTTAACTG TGCTGTGCAT 
CTGTGTTCTC TTTTCCACCA GCGATATGCT GACTTTGCAC AGTCTCTCCT TCAGGTATGG 
AAGAAGCATT TTGAAGCCCG CAAAGAGGAA AAGACTCCTA ATATTACTAA GCTGAGAACA 
GACTTGCGCT TTATTGCAGA GCTAACCATC ATTGGCATCT TTACTGATAA AGAAGGCCTC 
TCTCTTATTT ATGAGCAACT TAAAAACATA ATTAATGCTG ATCGAGAATC CCACACACAT 
GTTTCAGTAG TAATCAGTTT TTGCCGGCAC TGTGGAGATG ATATTGCAGG CTTAGTGCCC 
AGGAAAGTGA AGTGTGCAGC TGAAAAATAC AATCTTAATT TTCCTCCTAG TGAGATTATC 
AGCCCAGAGA AACAACAGCC TTTCCAGAAT TTGCTGAAAG AGTATTTCAC ATCTCTGACG 
AAACACTTGA AGAGGGACCA CAGAGAATTA CAAAATATAG AGCGCCAGAA CAGGCGCATT 
CTTCACACTA AAGGGGAGCT AAGTGAAGAC AGGCACAAGC AATATGAGGA GTTTGCAACA 
TCTTATCAGA AGCTGTTAGC TAGCTGTCAA TCACTTGCAG ATCTGCTAGA TGAAAATATG 
CCAGAACTTC CTCAGGACAA ACCTGCTCCG GAGGAACATG GGCCTGGTAT TGATATCTTC 
ACACCTGGAA AACCTGGAGA TTATGACTTA GAAGGAGGCA TTTGGGAAGA CGAGGATGCC 
CGTAACTTTT ATGAGAATCT GATTGATTTG AAAGCTTTTG TCCCAGCCAT TTTATTTAAG 
GATAATGAGA AAAATTCTCA GAGTAAAGAA ACTGGCAAAG ACAGTTTCAA AGATAGTAAA 
GAGAAAGATG TGTCCACAAC TGAAGAGCTA GAGCTGGAGT TAGAGACATT GGATATTAAT 
GATGAAGGCA TGGAACTGGA AGGTGCCGAA GATGCAGAAG ACCTAACAAA AAAACTTTTG 
GATGAACAAG AGCAAGAAGA TGAAGAGGCA AGCACAGGCT CCCATTTAAA ACTCATTGTT 
GATGCTTTTA TTCAGCAGCT GCCAAACTGT GTTAATCGTG ATCTAATAGA TAAGGCTGCA 
ATGGATTTTT GCATGAACAT GAATACAAAA GCTAATAGAA GGAAATTGGT CAGAGCACTA 
TTCATTGTTC CCAGACAAAG ACTGGATCTT CTTCCATTCT ATGCCAGATT AGTGGCCACC 
TTGCATCCTT GCATGTCTGA TGTTGCAGAA GACCTTTGTT CCATGTTAAA GGGAGACTTT 
CGTTTCCACG TTAGAAAGAA GGATCAAATC AACATTGAAA CAAAGAACAA GACTGTGAGA 
TTTATTGGAG AACTTGCCAA ATTTAAAATG TTCAGCAAAA CAGATACGCT GCATTGTTTA 
AAGATGCTGT TGTCAGACTT TTCGCATCAC CACATAGAAA TGGCATGCAC TCTACTGGAA 
ACTTGTGGCA GATTTCTTTT TCGATCTCCA GATTCTCACT TGCGCACCAG TGTCCTGCTG 
GAGCAAATGA TGCGCAAGAA GCAAGCAATG CACTTAGATG CACGCTATGT TACAATGGTT 
GAAAATGCCT ACTATTACTG TAACCCACCT CCAGCTGAAA AAACTGTGAA AAAGAAGCGA 
CCTCCACTAC AAGAATACAT CAGAAAGCTT CTTTATAAAG ACCTTTCCAA GGTCACCACT 
GAAAAGGTTT TGAGGCAGAT GAGGAAGCTG TCTTGGCAGG ATCCTGAAGT GAAGGACTAT 
GTAATTTGCT GCATGATCAA CATCTGGAAT GTTAAATACA ACAGCATTCA TTGTGTAGCC 
AATCTTTTGG CAGGACTGGT ACTTTACCAA GAGGATGTTG GAATACATGT TGTAGATGGA 
GTGTTAGAAG ATATACGTCT TGGGATGGAG GTTAACCAGC CAAAATTTAA TCAGCGGAGG 
ATAAGCAGTG CAAAGTTCCT TGGGGAGCTT TACAACTATA GAATGGTGGA ATCTGCAGTT 
ATATTTCGCA CACTTTATTC ATTTATTTCC TTTGGTGTAA ATTCTGATGG AAGCCCCAGC 
CCACTTGATC CTCCTGAACA CTTGTTCCGC ATTCGATTAG TTTGCACTAT CCTTGACACT 
TGTGGCCAGT ATTTTGACCG TGGATCAAGC AAGAGAAAGC TTGATTGTTT TCTTGTTTAT 
TTTCAGCGCT ACGTTTGGTG GAAGAAAAGT CTTGATGTGT GGACTAAAGA CCACCCATTT 
CCAATAGATA TTGATTATAT GATAAGTGAT ACCTTGGAGC TTCTGAGGCC AAAAATGAAG 
CTCTGTGCCT CACTGAATGA ATCTGTCCGC CAAGTGCAGG ATTTGGAGCG TGAGTTTCTG 
ACAAAGCTAG GTCTCATGAA TGATAAGGAA TCCAAACATT CCATGACAGA AGGAGATCAC 
CTAGTGTATG AAGATGATGA TGAGGAAGAA GAGGCTGGGG CAGAGACAGA GGAGCAGTCT 
GGCAATGAAA GTGAAATGAA TGAACAAGAA GAAGATGCTT CTGAAAATGA AGAGGATGAA 
AGAGAGGAGG AAGAAGAGGA GAATACAGAT TATATGACTG ACTCAAACAA AGAAAATGAA 
ACAGATGAAG AAAACACTGA GGTGATGATT AAAGGAGGTG GAATTAAACA TGTACCCTGT 
GCTGAAGATG AAGACTTTAT TCAAGCCCTT GACAAGATGA TGCTGGAAAA TCTTCAGCAA 
CGTAGTGGAG AGTCAGTGAA GGTGCATCAG TTAGATGTTG CTATTCCCCT GCATCTTAAG 
AGCCAGCTTA AAAAGGGACC AGCTATGAGC ACTGGAGAAG TAGACCCGGA ATCTGGCGAC 
ACTATGCCAT TTGTTATGCT TACAAGAAGA GGTAACAAAC AGCAGTTCAA GATCCTTAAT 
GTGCCTATGT CCTCCCAACT GGCTGCAAAT CACTGGAGTC AGCAGCAAGC TGAACAAGAA 
GAGAGGATGA GGATGAAGAA ACTCACATTA GATATCAATG AGCGTCAGGA ACAAGAAGAT 
TACCAAGAAA TGTTGCAGTC ATTGGCTCAG CGCCCAGCAC CTGCCAACAC AAACAGGGAG 
CGCAGGCCTC GATACCAGCA TCCAAAGGGA GCACCTAACG CAGACCTCAT CTTCAAAACT 
GGGGGCAGGA GACGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814094.1
CDS	400..4197
Translation	MPAERKKPAGMEEKESPSSPRDKECAEKKSTSSKDRVKEEPKASTKRDQSKASEEKKKRTDEDKRKKEEKECKRKEDDQVKADDEQQSAEGKNQEDEIKKKQEEEEAHQREAAALQIKEKDEANQLHQEAWERHHTRKELRSKNQNAPENRPEESFFSRLDSSLKKNTAFVKKLKTITEQQRDSLSHDFNSLNLSKYIAEAAASIVEAKLKISDVNCAVHLCSLFHQRYADFAQSLLQVWKKHFEARKEEKTPNITKLRTDLRFIAELTIIGIFTDKEGLSLIYEQLKNIINADRESHTHVSVVISFCRHCGDDIAGLVPRKVKCAAEKYNLNFPPSEIISPEKQQPFQNLLKEYFTSLTKHLKRDHRELQNIERQNRRILHTKGELSEDRHKQYEEFATSYQKLLASCQSLADLLDENMPELPQDKPAPEEHGPGIDIFTPGKPGDYDLEGGIWEDEDARNFYENLIDLKAFVPAILFKDNEKNSQSKETGKDSFKDSKEKDVSTTEELELELETLDINDEGMELEGAEDAEDLTKKLLDEQEQEDEEASTGSHLKLIVDAFIQQLPNCVNRDLIDKAAMDFCMNMNTKANRRKLVRALFIVPRQRLDLLPFYARLVATLHPCMSDVAEDLCSMLKGDFRFHVRKKDQINIETKNKTVRFIGELAKFKMFSKTDTLHCLKMLLSDFSHHHIEMACTLLETCGRFLFRSPDSHLRTSVLLEQMMRKKQAMHLDARYVTMVENAYYYCNPPPAEKTVKKKRPPLQEYIRKLLYKDLSKVTTEKVLRQMRKLSWQDPEVKDYVICCMINIWNVKYNSIHCVANLLAGLVLYQEDVGIHVVDGVLEDIRLGMEVNQPKFNQRRISSAKFLGELYNYRMVESAVIFRTLYSFISFGVNSDGSPSPLDPPEHLFRIRLVCTILDTCGQYFDRGSSKRKLDCFLVYFQRYVWWKKSLDVWTKDHPFPIDIDYMISDTLELLRPKMKLCASLNESVRQVQDLEREFLTKLGLMNDKESKHSMTEGDHLVYEDDDEEEEAGAETEEQSGNESEMNEQEEDASENEEDEREEEEEENTDYMTDSNKENETDEENTEVMIKGGGIKHVPCAEDEDFIQALDKMMLENLQQRSGESVKVHQLDVAIPLHLKSQLKKGPAMSTGEVDPESGDTMPFVMLTRRGNKQQFKILNVPMSSQLAANHWSQQQAEQEERMRMKKLTLDINERQEQEDYQEMLQSLAQRPAPANTNRERRPRYQHPKGAPNADLIFKTGGRRR

Target ORF information:

RefSeq Version	XM_012958640.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis UPF2 regulator of nonsense transcripts homolog (upf2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012958640.2

ORF Insert Sequence:

ATGCCAGCAG AACGCAAGAA ACCAGCAGGT ATGGAAGAAA AGGAATCTCC ATCTAGCCCA 
AGGGACAAAG AATGTGCTGA GAAGAAATCT ACAAGCAGCA AAGATCGGGT TAAGGAAGAA 
CCAAAAGCAA GCACCAAAAG AGATCAATCA AAGGCTTCTG AGGAAAAAAA GAAGCGAACT 
GATGAGGACA AGCGGAAAAA AGAGGAGAAG GAGTGCAAAA GAAAGGAGGA TGATCAAGTC 
AAAGCAGATG ATGAGCAGCA AAGTGCAGAG GGAAAAAATC AAGAAGATGA AATAAAAAAG 
AAGCAGGAAG AAGAGGAAGC TCATCAGAGA GAAGCTGCAG CATTGCAAAT CAAAGAGAAG 
GATGAAGCAA ATCAGTTACA CCAAGAAGCA TGGGAACGGC ACCACACTAG GAAGGAGCTT 
CGTAGCAAAA ATCAAAATGC CCCTGAAAAT CGACCTGAGG AGAGTTTCTT TAGCAGGCTG 
GACTCTAGTT TGAAGAAGAA CACTGCTTTT GTGAAGAAAC TCAAAACCAT TACTGAACAG 
CAAAGAGATT CATTGTCCCA TGACTTCAAC AGCCTTAACC TCAGCAAGTA CATAGCAGAG 
GCAGCAGCCT CCATTGTAGA GGCCAAGTTA AAAATATCTG ATGTTAACTG TGCTGTGCAT 
CTGTGTTCTC TTTTCCACCA GCGATATGCT GACTTTGCAC AGTCTCTCCT TCAGGTATGG 
AAGAAGCATT TTGAAGCCCG CAAAGAGGAA AAGACTCCTA ATATTACTAA GCTGAGAACA 
GACTTGCGCT TTATTGCAGA GCTAACCATC ATTGGCATCT TTACTGATAA AGAAGGCCTC 
TCTCTTATTT ATGAGCAACT TAAAAACATA ATTAATGCTG ATCGAGAATC CCACACACAT 
GTTTCAGTAG TAATCAGTTT TTGCCGGCAC TGTGGAGATG ATATTGCAGG CTTAGTGCCC 
AGGAAAGTGA AGTGTGCAGC TGAAAAATAC AATCTTAATT TTCCTCCTAG TGAGATTATC 
AGCCCAGAGA AACAACAGCC TTTCCAGAAT TTGCTGAAAG AGTATTTCAC ATCTCTGACG 
AAACACTTGA AGAGGGACCA CAGAGAATTA CAAAATATAG AGCGCCAGAA CAGGCGCATT 
CTTCACACTA AAGGGGAGCT AAGTGAAGAC AGGCACAAGC AATATGAGGA GTTTGCAACA 
TCTTATCAGA AGCTGTTAGC TAGCTGTCAA TCACTTGCAG ATCTGCTAGA TGAAAATATG 
CCAGAACTTC CTCAGGACAA ACCTGCTCCG GAGGAACATG GGCCTGGTAT TGATATCTTC 
ACACCTGGAA AACCTGGAGA TTATGACTTA GAAGGAGGCA TTTGGGAAGA CGAGGATGCC 
CGTAACTTTT ATGAGAATCT GATTGATTTG AAAGCTTTTG TCCCAGCCAT TTTATTTAAG 
GATAATGAGA AAAATTCTCA GAGTAAAGAA ACTGGCAAAG ACAGTTTCAA AGATAGTAAA 
GAGAAAGATG TGTCCACAAC TGAAGAGCTA GAGCTGGAGT TAGAGACATT GGATATTAAT 
GATGAAGGCA TGGAACTGGA AGGTGCCGAA GATGCAGAAG ACCTAACAAA AAAACTTTTG 
GATGAACAAG AGCAAGAAGA TGAAGAGGCA AGCACAGGCT CCCATTTAAA ACTCATTGTT 
GATGCTTTTA TTCAGCAGCT GCCAAACTGT GTTAATCGTG ATCTAATAGA TAAGGCTGCA 
ATGGATTTTT GCATGAACAT GAATACAAAA GCTAATAGAA GGAAATTGGT CAGAGCACTA 
TTCATTGTTC CCAGACAAAG ACTGGATCTT CTTCCATTCT ATGCCAGATT AGTGGCCACC 
TTGCATCCTT GCATGTCTGA TGTTGCAGAA GACCTTTGTT CCATGTTAAA GGGAGACTTT 
CGTTTCCACG TTAGAAAGAA GGATCAAATC AACATTGAAA CAAAGAACAA GACTGTGAGA 
TTTATTGGAG AACTTGCCAA ATTTAAAATG TTCAGCAAAA CAGATACGCT GCATTGTTTA 
AAGATGCTGT TGTCAGACTT TTCGCATCAC CACATAGAAA TGGCATGCAC TCTACTGGAA 
ACTTGTGGCA GATTTCTTTT TCGATCTCCA GATTCTCACT TGCGCACCAG TGTCCTGCTG 
GAGCAAATGA TGCGCAAGAA GCAAGCAATG CACTTAGATG CACGCTATGT TACAATGGTT 
GAAAATGCCT ACTATTACTG TAACCCACCT CCAGCTGAAA AAACTGTGAA AAAGAAGCGA 
CCTCCACTAC AAGAATACAT CAGAAAGCTT CTTTATAAAG ACCTTTCCAA GGTCACCACT 
GAAAAGGTTT TGAGGCAGAT GAGGAAGCTG TCTTGGCAGG ATCCTGAAGT GAAGGACTAT 
GTAATTTGCT GCATGATCAA CATCTGGAAT GTTAAATACA ACAGCATTCA TTGTGTAGCC 
AATCTTTTGG CAGGACTGGT ACTTTACCAA GAGGATGTTG GAATACATGT TGTAGATGGA 
GTGTTAGAAG ATATACGTCT TGGGATGGAG GTTAACCAGC CAAAATTTAA TCAGCGGAGG 
ATAAGCAGTG CAAAGTTCCT TGGGGAGCTT TACAACTATA GAATGGTGGA ATCTGCAGTT 
ATATTTCGCA CACTTTATTC ATTTATTTCC TTTGGTGTAA ATTCTGATGG AAGCCCCAGC 
CCACTTGATC CTCCTGAACA CTTGTTCCGC ATTCGATTAG TTTGCACTAT CCTTGACACT 
TGTGGCCAGT ATTTTGACCG TGGATCAAGC AAGAGAAAGC TTGATTGTTT TCTTGTTTAT 
TTTCAGCGCT ACGTTTGGTG GAAGAAAAGT CTTGATGTGT GGACTAAAGA CCACCCATTT 
CCAATAGATA TTGATTATAT GATAAGTGAT ACCTTGGAGC TTCTGAGGCC AAAAATGAAG 
CTCTGTGCCT CACTGAATGA ATCTGTCCGC CAAGTGCAGG ATTTGGAGCG TGAGTTTCTG 
ACAAAGCTAG GTCTCATGAA TGATAAGGAA TCCAAACATT CCATGACAGA AGGAGATCAC 
CTAGTGTATG AAGATGATGA TGAGGAAGAA GAGGCTGGGG CAGAGACAGA GGAGCAGTCT 
GGCAATGAAA GTGAAATGAA TGAACAAGAA GAAGATGCTT CTGAAAATGA AGAGGATGAA 
AGAGAGGAGG AAGAAGAGGA GAATACAGAT TATATGACTG ACTCAAACAA AGAAAATGAA 
ACAGATGAAG AAAACACTGA GGTGATGATT AAAGGAGGTG GAATTAAACA TGTACCCTGT 
GCTGAAGATG AAGACTTTAT TCAAGCCCTT GACAAGATGA TGCTGGAAAA TCTTCAGCAA 
CGTAGTGGAG AGTCAGTGAA GGTGCATCAG TTAGATGTTG CTATTCCCCT GCATCTTAAG 
AGCCAGCTTA AAAAGGGACC AGCTATGAGC ACTGGAGAAG TAGACCCGGA ATCTGGCGAC 
ACTATGCCAT TTGTTATGCT TACAAGAAGA GGTAACAAAC AGCAGTTCAA GATCCTTAAT 
GTGCCTATGT CCTCCCAACT GGCTGCAAAT CACTGGAGTC AGCAGCAAGC TGAACAAGAA 
GAGAGGATGA GGATGAAGAA ACTCACATTA GATATCAATG AGCGTCAGGA ACAAGAAGAT 
TACCAAGAAA TGTTGCAGTC ATTGGCTCAG CGCCCAGCAC CTGCCAACAC AAACAGGGAG 
CGCAGGCCTC GATACCAGCA TCCAAAGGGA GCACCTAACG CAGACCTCAT CTTCAAAACT 
GGGGGCAGGA GACGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

upf2 ( XM_012958639.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814093.1 Xenopus tropicalis UPF2 regulator of nonsense transcripts homolog (upf2), transcript variant X1, mRNA.

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CloneID	OXa32206
Clone ID Related Accession (Same CDS sequence)	XM_012958640.2 , XM_012958639.2
Accession Version	XM_012958639.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	regulator of nonsense transcripts 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012958639.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGCAG AACGCAAGAA ACCAGCAGGT ATGGAAGAAA AGGAATCTCC ATCTAGCCCA 
AGGGACAAAG AATGTGCTGA GAAGAAATCT ACAAGCAGCA AAGATCGGGT TAAGGAAGAA 
CCAAAAGCAA GCACCAAAAG AGATCAATCA AAGGCTTCTG AGGAAAAAAA GAAGCGAACT 
GATGAGGACA AGCGGAAAAA AGAGGAGAAG GAGTGCAAAA GAAAGGAGGA TGATCAAGTC 
AAAGCAGATG ATGAGCAGCA AAGTGCAGAG GGAAAAAATC AAGAAGATGA AATAAAAAAG 
AAGCAGGAAG AAGAGGAAGC TCATCAGAGA GAAGCTGCAG CATTGCAAAT CAAAGAGAAG 
GATGAAGCAA ATCAGTTACA CCAAGAAGCA TGGGAACGGC ACCACACTAG GAAGGAGCTT 
CGTAGCAAAA ATCAAAATGC CCCTGAAAAT CGACCTGAGG AGAGTTTCTT TAGCAGGCTG 
GACTCTAGTT TGAAGAAGAA CACTGCTTTT GTGAAGAAAC TCAAAACCAT TACTGAACAG 
CAAAGAGATT CATTGTCCCA TGACTTCAAC AGCCTTAACC TCAGCAAGTA CATAGCAGAG 
GCAGCAGCCT CCATTGTAGA GGCCAAGTTA AAAATATCTG ATGTTAACTG TGCTGTGCAT 
CTGTGTTCTC TTTTCCACCA GCGATATGCT GACTTTGCAC AGTCTCTCCT TCAGGTATGG 
AAGAAGCATT TTGAAGCCCG CAAAGAGGAA AAGACTCCTA ATATTACTAA GCTGAGAACA 
GACTTGCGCT TTATTGCAGA GCTAACCATC ATTGGCATCT TTACTGATAA AGAAGGCCTC 
TCTCTTATTT ATGAGCAACT TAAAAACATA ATTAATGCTG ATCGAGAATC CCACACACAT 
GTTTCAGTAG TAATCAGTTT TTGCCGGCAC TGTGGAGATG ATATTGCAGG CTTAGTGCCC 
AGGAAAGTGA AGTGTGCAGC TGAAAAATAC AATCTTAATT TTCCTCCTAG TGAGATTATC 
AGCCCAGAGA AACAACAGCC TTTCCAGAAT TTGCTGAAAG AGTATTTCAC ATCTCTGACG 
AAACACTTGA AGAGGGACCA CAGAGAATTA CAAAATATAG AGCGCCAGAA CAGGCGCATT 
CTTCACACTA AAGGGGAGCT AAGTGAAGAC AGGCACAAGC AATATGAGGA GTTTGCAACA 
TCTTATCAGA AGCTGTTAGC TAGCTGTCAA TCACTTGCAG ATCTGCTAGA TGAAAATATG 
CCAGAACTTC CTCAGGACAA ACCTGCTCCG GAGGAACATG GGCCTGGTAT TGATATCTTC 
ACACCTGGAA AACCTGGAGA TTATGACTTA GAAGGAGGCA TTTGGGAAGA CGAGGATGCC 
CGTAACTTTT ATGAGAATCT GATTGATTTG AAAGCTTTTG TCCCAGCCAT TTTATTTAAG 
GATAATGAGA AAAATTCTCA GAGTAAAGAA ACTGGCAAAG ACAGTTTCAA AGATAGTAAA 
GAGAAAGATG TGTCCACAAC TGAAGAGCTA GAGCTGGAGT TAGAGACATT GGATATTAAT 
GATGAAGGCA TGGAACTGGA AGGTGCCGAA GATGCAGAAG ACCTAACAAA AAAACTTTTG 
GATGAACAAG AGCAAGAAGA TGAAGAGGCA AGCACAGGCT CCCATTTAAA ACTCATTGTT 
GATGCTTTTA TTCAGCAGCT GCCAAACTGT GTTAATCGTG ATCTAATAGA TAAGGCTGCA 
ATGGATTTTT GCATGAACAT GAATACAAAA GCTAATAGAA GGAAATTGGT CAGAGCACTA 
TTCATTGTTC CCAGACAAAG ACTGGATCTT CTTCCATTCT ATGCCAGATT AGTGGCCACC 
TTGCATCCTT GCATGTCTGA TGTTGCAGAA GACCTTTGTT CCATGTTAAA GGGAGACTTT 
CGTTTCCACG TTAGAAAGAA GGATCAAATC AACATTGAAA CAAAGAACAA GACTGTGAGA 
TTTATTGGAG AACTTGCCAA ATTTAAAATG TTCAGCAAAA CAGATACGCT GCATTGTTTA 
AAGATGCTGT TGTCAGACTT TTCGCATCAC CACATAGAAA TGGCATGCAC TCTACTGGAA 
ACTTGTGGCA GATTTCTTTT TCGATCTCCA GATTCTCACT TGCGCACCAG TGTCCTGCTG 
GAGCAAATGA TGCGCAAGAA GCAAGCAATG CACTTAGATG CACGCTATGT TACAATGGTT 
GAAAATGCCT ACTATTACTG TAACCCACCT CCAGCTGAAA AAACTGTGAA AAAGAAGCGA 
CCTCCACTAC AAGAATACAT CAGAAAGCTT CTTTATAAAG ACCTTTCCAA GGTCACCACT 
GAAAAGGTTT TGAGGCAGAT GAGGAAGCTG TCTTGGCAGG ATCCTGAAGT GAAGGACTAT 
GTAATTTGCT GCATGATCAA CATCTGGAAT GTTAAATACA ACAGCATTCA TTGTGTAGCC 
AATCTTTTGG CAGGACTGGT ACTTTACCAA GAGGATGTTG GAATACATGT TGTAGATGGA 
GTGTTAGAAG ATATACGTCT TGGGATGGAG GTTAACCAGC CAAAATTTAA TCAGCGGAGG 
ATAAGCAGTG CAAAGTTCCT TGGGGAGCTT TACAACTATA GAATGGTGGA ATCTGCAGTT 
ATATTTCGCA CACTTTATTC ATTTATTTCC TTTGGTGTAA ATTCTGATGG AAGCCCCAGC 
CCACTTGATC CTCCTGAACA CTTGTTCCGC ATTCGATTAG TTTGCACTAT CCTTGACACT 
TGTGGCCAGT ATTTTGACCG TGGATCAAGC AAGAGAAAGC TTGATTGTTT TCTTGTTTAT 
TTTCAGCGCT ACGTTTGGTG GAAGAAAAGT CTTGATGTGT GGACTAAAGA CCACCCATTT 
CCAATAGATA TTGATTATAT GATAAGTGAT ACCTTGGAGC TTCTGAGGCC AAAAATGAAG 
CTCTGTGCCT CACTGAATGA ATCTGTCCGC CAAGTGCAGG ATTTGGAGCG TGAGTTTCTG 
ACAAAGCTAG GTCTCATGAA TGATAAGGAA TCCAAACATT CCATGACAGA AGGAGATCAC 
CTAGTGTATG AAGATGATGA TGAGGAAGAA GAGGCTGGGG CAGAGACAGA GGAGCAGTCT 
GGCAATGAAA GTGAAATGAA TGAACAAGAA GAAGATGCTT CTGAAAATGA AGAGGATGAA 
AGAGAGGAGG AAGAAGAGGA GAATACAGAT TATATGACTG ACTCAAACAA AGAAAATGAA 
ACAGATGAAG AAAACACTGA GGTGATGATT AAAGGAGGTG GAATTAAACA TGTACCCTGT 
GCTGAAGATG AAGACTTTAT TCAAGCCCTT GACAAGATGA TGCTGGAAAA TCTTCAGCAA 
CGTAGTGGAG AGTCAGTGAA GGTGCATCAG TTAGATGTTG CTATTCCCCT GCATCTTAAG 
AGCCAGCTTA AAAAGGGACC AGCTATGAGC ACTGGAGAAG TAGACCCGGA ATCTGGCGAC 
ACTATGCCAT TTGTTATGCT TACAAGAAGA GGTAACAAAC AGCAGTTCAA GATCCTTAAT 
GTGCCTATGT CCTCCCAACT GGCTGCAAAT CACTGGAGTC AGCAGCAAGC TGAACAAGAA 
GAGAGGATGA GGATGAAGAA ACTCACATTA GATATCAATG AGCGTCAGGA ACAAGAAGAT 
TACCAAGAAA TGTTGCAGTC ATTGGCTCAG CGCCCAGCAC CTGCCAACAC AAACAGGGAG 
CGCAGGCCTC GATACCAGCA TCCAAAGGGA GCACCTAACG CAGACCTCAT CTTCAAAACT 
GGGGGCAGGA GACGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814093.1
CDS	388..4185
Translation	MPAERKKPAGMEEKESPSSPRDKECAEKKSTSSKDRVKEEPKASTKRDQSKASEEKKKRTDEDKRKKEEKECKRKEDDQVKADDEQQSAEGKNQEDEIKKKQEEEEAHQREAAALQIKEKDEANQLHQEAWERHHTRKELRSKNQNAPENRPEESFFSRLDSSLKKNTAFVKKLKTITEQQRDSLSHDFNSLNLSKYIAEAAASIVEAKLKISDVNCAVHLCSLFHQRYADFAQSLLQVWKKHFEARKEEKTPNITKLRTDLRFIAELTIIGIFTDKEGLSLIYEQLKNIINADRESHTHVSVVISFCRHCGDDIAGLVPRKVKCAAEKYNLNFPPSEIISPEKQQPFQNLLKEYFTSLTKHLKRDHRELQNIERQNRRILHTKGELSEDRHKQYEEFATSYQKLLASCQSLADLLDENMPELPQDKPAPEEHGPGIDIFTPGKPGDYDLEGGIWEDEDARNFYENLIDLKAFVPAILFKDNEKNSQSKETGKDSFKDSKEKDVSTTEELELELETLDINDEGMELEGAEDAEDLTKKLLDEQEQEDEEASTGSHLKLIVDAFIQQLPNCVNRDLIDKAAMDFCMNMNTKANRRKLVRALFIVPRQRLDLLPFYARLVATLHPCMSDVAEDLCSMLKGDFRFHVRKKDQINIETKNKTVRFIGELAKFKMFSKTDTLHCLKMLLSDFSHHHIEMACTLLETCGRFLFRSPDSHLRTSVLLEQMMRKKQAMHLDARYVTMVENAYYYCNPPPAEKTVKKKRPPLQEYIRKLLYKDLSKVTTEKVLRQMRKLSWQDPEVKDYVICCMINIWNVKYNSIHCVANLLAGLVLYQEDVGIHVVDGVLEDIRLGMEVNQPKFNQRRISSAKFLGELYNYRMVESAVIFRTLYSFISFGVNSDGSPSPLDPPEHLFRIRLVCTILDTCGQYFDRGSSKRKLDCFLVYFQRYVWWKKSLDVWTKDHPFPIDIDYMISDTLELLRPKMKLCASLNESVRQVQDLEREFLTKLGLMNDKESKHSMTEGDHLVYEDDDEEEEAGAETEEQSGNESEMNEQEEDASENEEDEREEEEEENTDYMTDSNKENETDEENTEVMIKGGGIKHVPCAEDEDFIQALDKMMLENLQQRSGESVKVHQLDVAIPLHLKSQLKKGPAMSTGEVDPESGDTMPFVMLTRRGNKQQFKILNVPMSSQLAANHWSQQQAEQEERMRMKKLTLDINERQEQEDYQEMLQSLAQRPAPANTNRERRPRYQHPKGAPNADLIFKTGGRRR

Target ORF information:

RefSeq Version	XM_012958639.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis UPF2 regulator of nonsense transcripts homolog (upf2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012958639.2

ORF Insert Sequence:

ATGCCAGCAG AACGCAAGAA ACCAGCAGGT ATGGAAGAAA AGGAATCTCC ATCTAGCCCA 
AGGGACAAAG AATGTGCTGA GAAGAAATCT ACAAGCAGCA AAGATCGGGT TAAGGAAGAA 
CCAAAAGCAA GCACCAAAAG AGATCAATCA AAGGCTTCTG AGGAAAAAAA GAAGCGAACT 
GATGAGGACA AGCGGAAAAA AGAGGAGAAG GAGTGCAAAA GAAAGGAGGA TGATCAAGTC 
AAAGCAGATG ATGAGCAGCA AAGTGCAGAG GGAAAAAATC AAGAAGATGA AATAAAAAAG 
AAGCAGGAAG AAGAGGAAGC TCATCAGAGA GAAGCTGCAG CATTGCAAAT CAAAGAGAAG 
GATGAAGCAA ATCAGTTACA CCAAGAAGCA TGGGAACGGC ACCACACTAG GAAGGAGCTT 
CGTAGCAAAA ATCAAAATGC CCCTGAAAAT CGACCTGAGG AGAGTTTCTT TAGCAGGCTG 
GACTCTAGTT TGAAGAAGAA CACTGCTTTT GTGAAGAAAC TCAAAACCAT TACTGAACAG 
CAAAGAGATT CATTGTCCCA TGACTTCAAC AGCCTTAACC TCAGCAAGTA CATAGCAGAG 
GCAGCAGCCT CCATTGTAGA GGCCAAGTTA AAAATATCTG ATGTTAACTG TGCTGTGCAT 
CTGTGTTCTC TTTTCCACCA GCGATATGCT GACTTTGCAC AGTCTCTCCT TCAGGTATGG 
AAGAAGCATT TTGAAGCCCG CAAAGAGGAA AAGACTCCTA ATATTACTAA GCTGAGAACA 
GACTTGCGCT TTATTGCAGA GCTAACCATC ATTGGCATCT TTACTGATAA AGAAGGCCTC 
TCTCTTATTT ATGAGCAACT TAAAAACATA ATTAATGCTG ATCGAGAATC CCACACACAT 
GTTTCAGTAG TAATCAGTTT TTGCCGGCAC TGTGGAGATG ATATTGCAGG CTTAGTGCCC 
AGGAAAGTGA AGTGTGCAGC TGAAAAATAC AATCTTAATT TTCCTCCTAG TGAGATTATC 
AGCCCAGAGA AACAACAGCC TTTCCAGAAT TTGCTGAAAG AGTATTTCAC ATCTCTGACG 
AAACACTTGA AGAGGGACCA CAGAGAATTA CAAAATATAG AGCGCCAGAA CAGGCGCATT 
CTTCACACTA AAGGGGAGCT AAGTGAAGAC AGGCACAAGC AATATGAGGA GTTTGCAACA 
TCTTATCAGA AGCTGTTAGC TAGCTGTCAA TCACTTGCAG ATCTGCTAGA TGAAAATATG 
CCAGAACTTC CTCAGGACAA ACCTGCTCCG GAGGAACATG GGCCTGGTAT TGATATCTTC 
ACACCTGGAA AACCTGGAGA TTATGACTTA GAAGGAGGCA TTTGGGAAGA CGAGGATGCC 
CGTAACTTTT ATGAGAATCT GATTGATTTG AAAGCTTTTG TCCCAGCCAT TTTATTTAAG 
GATAATGAGA AAAATTCTCA GAGTAAAGAA ACTGGCAAAG ACAGTTTCAA AGATAGTAAA 
GAGAAAGATG TGTCCACAAC TGAAGAGCTA GAGCTGGAGT TAGAGACATT GGATATTAAT 
GATGAAGGCA TGGAACTGGA AGGTGCCGAA GATGCAGAAG ACCTAACAAA AAAACTTTTG 
GATGAACAAG AGCAAGAAGA TGAAGAGGCA AGCACAGGCT CCCATTTAAA ACTCATTGTT 
GATGCTTTTA TTCAGCAGCT GCCAAACTGT GTTAATCGTG ATCTAATAGA TAAGGCTGCA 
ATGGATTTTT GCATGAACAT GAATACAAAA GCTAATAGAA GGAAATTGGT CAGAGCACTA 
TTCATTGTTC CCAGACAAAG ACTGGATCTT CTTCCATTCT ATGCCAGATT AGTGGCCACC 
TTGCATCCTT GCATGTCTGA TGTTGCAGAA GACCTTTGTT CCATGTTAAA GGGAGACTTT 
CGTTTCCACG TTAGAAAGAA GGATCAAATC AACATTGAAA CAAAGAACAA GACTGTGAGA 
TTTATTGGAG AACTTGCCAA ATTTAAAATG TTCAGCAAAA CAGATACGCT GCATTGTTTA 
AAGATGCTGT TGTCAGACTT TTCGCATCAC CACATAGAAA TGGCATGCAC TCTACTGGAA 
ACTTGTGGCA GATTTCTTTT TCGATCTCCA GATTCTCACT TGCGCACCAG TGTCCTGCTG 
GAGCAAATGA TGCGCAAGAA GCAAGCAATG CACTTAGATG CACGCTATGT TACAATGGTT 
GAAAATGCCT ACTATTACTG TAACCCACCT CCAGCTGAAA AAACTGTGAA AAAGAAGCGA 
CCTCCACTAC AAGAATACAT CAGAAAGCTT CTTTATAAAG ACCTTTCCAA GGTCACCACT 
GAAAAGGTTT TGAGGCAGAT GAGGAAGCTG TCTTGGCAGG ATCCTGAAGT GAAGGACTAT 
GTAATTTGCT GCATGATCAA CATCTGGAAT GTTAAATACA ACAGCATTCA TTGTGTAGCC 
AATCTTTTGG CAGGACTGGT ACTTTACCAA GAGGATGTTG GAATACATGT TGTAGATGGA 
GTGTTAGAAG ATATACGTCT TGGGATGGAG GTTAACCAGC CAAAATTTAA TCAGCGGAGG 
ATAAGCAGTG CAAAGTTCCT TGGGGAGCTT TACAACTATA GAATGGTGGA ATCTGCAGTT 
ATATTTCGCA CACTTTATTC ATTTATTTCC TTTGGTGTAA ATTCTGATGG AAGCCCCAGC 
CCACTTGATC CTCCTGAACA CTTGTTCCGC ATTCGATTAG TTTGCACTAT CCTTGACACT 
TGTGGCCAGT ATTTTGACCG TGGATCAAGC AAGAGAAAGC TTGATTGTTT TCTTGTTTAT 
TTTCAGCGCT ACGTTTGGTG GAAGAAAAGT CTTGATGTGT GGACTAAAGA CCACCCATTT 
CCAATAGATA TTGATTATAT GATAAGTGAT ACCTTGGAGC TTCTGAGGCC AAAAATGAAG 
CTCTGTGCCT CACTGAATGA ATCTGTCCGC CAAGTGCAGG ATTTGGAGCG TGAGTTTCTG 
ACAAAGCTAG GTCTCATGAA TGATAAGGAA TCCAAACATT CCATGACAGA AGGAGATCAC 
CTAGTGTATG AAGATGATGA TGAGGAAGAA GAGGCTGGGG CAGAGACAGA GGAGCAGTCT 
GGCAATGAAA GTGAAATGAA TGAACAAGAA GAAGATGCTT CTGAAAATGA AGAGGATGAA 
AGAGAGGAGG AAGAAGAGGA GAATACAGAT TATATGACTG ACTCAAACAA AGAAAATGAA 
ACAGATGAAG AAAACACTGA GGTGATGATT AAAGGAGGTG GAATTAAACA TGTACCCTGT 
GCTGAAGATG AAGACTTTAT TCAAGCCCTT GACAAGATGA TGCTGGAAAA TCTTCAGCAA 
CGTAGTGGAG AGTCAGTGAA GGTGCATCAG TTAGATGTTG CTATTCCCCT GCATCTTAAG 
AGCCAGCTTA AAAAGGGACC AGCTATGAGC ACTGGAGAAG TAGACCCGGA ATCTGGCGAC 
ACTATGCCAT TTGTTATGCT TACAAGAAGA GGTAACAAAC AGCAGTTCAA GATCCTTAAT 
GTGCCTATGT CCTCCCAACT GGCTGCAAAT CACTGGAGTC AGCAGCAAGC TGAACAAGAA 
GAGAGGATGA GGATGAAGAA ACTCACATTA GATATCAATG AGCGTCAGGA ACAAGAAGAT 
TACCAAGAAA TGTTGCAGTC ATTGGCTCAG CGCCCAGCAC CTGCCAACAC AAACAGGGAG 
CGCAGGCCTC GATACCAGCA TCCAAAGGGA GCACCTAACG CAGACCTCAT CTTCAAAACT 
GGGGGCAGGA GACGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

upf2 ( XM_012958641.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814095.1 Xenopus tropicalis UPF2 regulator of nonsense transcripts homolog (upf2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa32207
Clone ID Related Accession (Same CDS sequence)	XM_012958641.2
Accession Version	XM_012958641.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	regulator of nonsense transcripts 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012958641.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGCAG AACGCAAGAA ACCAGCAGGT ATGGAAGAAA AGGAATCTCC ATCTAGCCCA 
AGGGACAAAG AATGTGCTGA GAAGAAATCT ACAAGCAGCA AAGATCGGGT TAAGGAAGAA 
CCAAAAGCAA GCACCAAAAG AGATCAATCA AAGGCTTCTG AGGAAAAAAA GAAGCGAACT 
GATGAGGACA AGCGGAAAAA AGAGGAGAAG GAGTGCAAAA GAAAGGAGGA TGATCAAGTC 
AAAGCAGATG ATGAGCAGCA AAGTGCAGAG GGAAAAAATC AAGAAGATGA AATAAAAAAG 
AAGCAGGAAG AAGAGGAAGC TCATCAGAGA GAAGCTGCAG CATTGCAAAT CAAAGAGAAG 
GATGAAGCAA ATCAGTTACA CCAAGAAGCA TGGGAACGGC ACCACACTAG GAAGGAGCTT 
CGTAGCAAAA ATCAAAATGC CCCTGAAAAT CGACCTGAGG AGAGTTTCTT TAGCAGGCTG 
GACTCTAGTT TGAAGAAGAA CACTGCTTTT GTGAAGAAAC TCAAAACCAT TACTGAACAG 
CAAAGAGATT CATTGTCCCA TGACTTCAAC AGCCTTAACC TCAGCAAGTA CATAGCAGAG 
GCAGCAGCCT CCATTGTAGA GGCCAAGTTA AAAATATCTG ATGTTAACTG TGCTGTGCAT 
CTGTGTTCTC TTTTCCACCA GCGATATGCT GACTTTGCAC AGTCTCTCCT TCAGGTATGG 
AAGAAGCATT TTGAAGCCCG CAAAGAGGAA AAGACTCCTA ATATTACTAA GCTGAGAACA 
GACTTGCGCT TTATTGCAGA GCTAACCATC ATTGGCATCT TTACTGATAA AGAAGGCCTC 
TCTCTTATTT ATGAGCAACT TAAAAACATA ATTAATGCTG ATCGAGAATC CCACACACAT 
GTTTCAGTAG TAATCAGTTT TTGCCGGCAC TGTGGAGATG ATATTGCAGG CTTAGTGCCC 
AGGAAAGTGA AGTGTGCAGC TGAAAAATAC AATCTTAATT TTCCTCCTAG TGAGATTATC 
AGCCCAGAGA AACAACAGCC TTTCCAGAAT TTGCTGAAAG AGTATTTCAC ATCTCTGACG 
AAACACTTGA AGAGGGACCA CAGAGAATTA CAAAATATAG AGCGCCAGAA CAGGCGCATT 
CTTCACACTA AAGGGGAGCT AAGTGAAGAC AGGCACAAGC AATATGAGGA GTTTGCAACA 
TCTTATCAGA AGCTGTTAGC TAGCTGTCAA TCACTTGCAG ATCTGCTAGA TGAAAATATG 
CCAGAACTTC CTCAGGACAA ACCTGCTCCG GAGGAACATG GGCCTGGTAT TGATATCTTC 
ACACCTGGAA AACCTGGAGA TTATGACTTA GAAGGAGGCA TTTGGGAAGA CGAGGATGCC 
CGTAACTTTT ATGAGAATCT GATTGATTTG AAAGCTTTTG TCCCAGCCAT TTTATTTAAG 
GATAATGAGA AAAATTCTCA GAGTAAAGAA ACTGGCAAAG ACAGTTTCAA AGATAGTAAA 
GAGAAAGATG TGTCCACAAC TGAAGAGCTA GAGCTGGAGT TAGAGACATT GGATATTAAT 
GATGAAGGCA TGGAACTGGA AGGTGCCGAA GATGCAGAAG ACCTAACAAA AAAACTTTTG 
GATGAACAAG AGCAAGAAGA TGAAGAGGCA AGCACAGGCT CCCATTTAAA ACTCATTGTT 
GATGCTTTTA TTCAGCAGCT GCCAAACTGT GTTAATCGTG ATCTAATAGA TAAGGCTGCA 
ATGGATTTTT GCATGAACAT GAATACAAAA GCTAATAGAA GGAAATTGGT CAGAGCACTA 
TTCATTGTTC CCAGACAAAG ACTGGATCTT CTTCCATTCT ATGCCAGATT AGTGGCCACC 
TTGCATCCTT GCATGTCTGA TGTTGCAGAA GACCTTTGTT CCATGTTAAA GGGAGACTTT 
CGTTTCCACG TTAGAAAGAA GGATCAAATC AACATTGAAA CAAAGAACAA GACTGTGAGA 
TTTATTGGAG AACTTGCCAA ATTTAAAATG TTCAGCAAAA CAGATACGCT GCATTGTTTA 
AAGATGCTGT TGTCAGACTT TTCGCATCAC CACATAGAAA TGGCATGCAC TCTACTGGAA 
ACTTGTGGCA GATTTCTTTT TCGATCTCCA GATTCTCACT TGCGCACCAG TGTCCTGCTG 
GAGCAAATGA TGCGCAAGAA GCAAGCAATG CACTTAGATG CACGCTATGT TACAATGGTT 
GAAAATGCCT ACTATTACTG TAACCCACCT CCAGCTGAAA AAACTGTGAA AAAGAAGCGA 
CCTCCACTAC AAGAATACAT CAGAAAGCTT CTTTATAAAG ACCTTTCCAA GGTCACCACT 
GAAAAGGTTT TGAGGCAGAT GAGGAAGCTG TCTTGGCAGG ATCCTGAAGT GAAGGACTAT 
GTAATTTGCT GCATGATCAA CATCTGGAAT GTTAAATACA ACAGCATTCA TTGTGTAGCC 
AATCTTTTGG CAGGACTGGT ACTTTACCAA GAGGATGTTG GAATACATGT TGTAGATGGA 
GTGTTAGAAG ATATACGTCT TGGGATGGAG GTTAACCAGC CAAAATTTAA TCAGCGGAGG 
ATAAGCAGTG CAAAGTTCCT TGGGGAGCTT TACAACTATA GAATGGTGGA ATCTGCAGTT 
ATATTTCGCA CACTTTATTC ATTTATTTCC TTTGGTGTAA ATTCTGATGG AAGCCCCAGC 
CCACTTGATC CTCCTGAACA CTTGTTCCGC ATTCGATTAG TTTGCACTAT CCTTGACACT 
TGTGGCCAGT ATTTTGACCG TGGATCAAGC AAGAGAAAGC TTGATTGTTT TCTTGTTTAT 
TTTCAGCGCT ACGTTTGGTG GAAGAAAAGT CTTGATGTGT GGACTAAAGA CCACCCATTT 
CCAATAGATA TTGATTATAT GATAAGTGAT ACCTTGGAGC TTCTGAGGCC AAAAATGAAG 
CTCTGTGCCT CACTGAATGA ATCTGTCCGC CAAGTGCAGG ATTTGGAGCG TGAGTTTCTG 
ACAAAGCTAG GTCTCATGAA TGATAAGGAA TCCAAACATT CCATGACAGA AGGAGATCAC 
CTAGTGTATG AAGATGATGA TGAGGAAGAA GAGGCTGGGG CAGAGACAGA GGAGCAGTCT 
GGCAATGAAA GTGAAATGAA TGAACAAGAA GAAGATGCTT CTGAAAATGA AGAGGATGAA 
AGAGAGGAGG AAGAAGAGGA GAATACAGAT TATATGACTG ACTCAAACAA AGAAAATGAA 
ACAGATGAAG AAAACACTGA GGTGATGATT AAAGGAGGTG GAATTAAACA TGTACCCTGT 
GCTGAAGATG AAGACTTTAT TCAAGCCCTT GACAAGATGA TGCTGGAAAA TCTTCAGCAA 
CGTAGTGGAG AGTCAGTGAA GGTGCATCAG TTAGATGTTG CTATTCCCCT GCATCTTAAG 
AGCCAGCTTA AAAAGGGACC AGCTATGAGC ACTGGAGAAG TAGACCCGGA ATCTGGCGAC 
ACTATGCCAT TTGTTATGCT TACAAGAAGA GGTAACAAAC AGCAGTTCAA GATCCTTAAT 
GTGCCTATGT CCTCCCAACT GGCTGCAAAT CACTGGAGTC AGCAGCAAGC TGAACAAGAA 
GAGAGGATGA GGATGAAGAA ACTCACATTA GATATCAATG AGCGTCAGGA ACAAGAAGAT 
TACCAAGAAA TGTTGCAGTC ATTGGCTCAG CGCCCAGCAC CTGCCAACAC AAACAGGGAG 
CGCAGGCCTC GATACCAGCA TCCAAAGGGA GCACCTAACG CAGACCTCAT CTTCAAAACT 
GGGGGCAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814095.1
CDS	392..4183
Translation	MPAERKKPAGMEEKESPSSPRDKECAEKKSTSSKDRVKEEPKASTKRDQSKASEEKKKRTDEDKRKKEEKECKRKEDDQVKADDEQQSAEGKNQEDEIKKKQEEEEAHQREAAALQIKEKDEANQLHQEAWERHHTRKELRSKNQNAPENRPEESFFSRLDSSLKKNTAFVKKLKTITEQQRDSLSHDFNSLNLSKYIAEAAASIVEAKLKISDVNCAVHLCSLFHQRYADFAQSLLQVWKKHFEARKEEKTPNITKLRTDLRFIAELTIIGIFTDKEGLSLIYEQLKNIINADRESHTHVSVVISFCRHCGDDIAGLVPRKVKCAAEKYNLNFPPSEIISPEKQQPFQNLLKEYFTSLTKHLKRDHRELQNIERQNRRILHTKGELSEDRHKQYEEFATSYQKLLASCQSLADLLDENMPELPQDKPAPEEHGPGIDIFTPGKPGDYDLEGGIWEDEDARNFYENLIDLKAFVPAILFKDNEKNSQSKETGKDSFKDSKEKDVSTTEELELELETLDINDEGMELEGAEDAEDLTKKLLDEQEQEDEEASTGSHLKLIVDAFIQQLPNCVNRDLIDKAAMDFCMNMNTKANRRKLVRALFIVPRQRLDLLPFYARLVATLHPCMSDVAEDLCSMLKGDFRFHVRKKDQINIETKNKTVRFIGELAKFKMFSKTDTLHCLKMLLSDFSHHHIEMACTLLETCGRFLFRSPDSHLRTSVLLEQMMRKKQAMHLDARYVTMVENAYYYCNPPPAEKTVKKKRPPLQEYIRKLLYKDLSKVTTEKVLRQMRKLSWQDPEVKDYVICCMINIWNVKYNSIHCVANLLAGLVLYQEDVGIHVVDGVLEDIRLGMEVNQPKFNQRRISSAKFLGELYNYRMVESAVIFRTLYSFISFGVNSDGSPSPLDPPEHLFRIRLVCTILDTCGQYFDRGSSKRKLDCFLVYFQRYVWWKKSLDVWTKDHPFPIDIDYMISDTLELLRPKMKLCASLNESVRQVQDLEREFLTKLGLMNDKESKHSMTEGDHLVYEDDDEEEEAGAETEEQSGNESEMNEQEEDASENEEDEREEEEEENTDYMTDSNKENETDEENTEVMIKGGGIKHVPCAEDEDFIQALDKMMLENLQQRSGESVKVHQLDVAIPLHLKSQLKKGPAMSTGEVDPESGDTMPFVMLTRRGNKQQFKILNVPMSSQLAANHWSQQQAEQEERMRMKKLTLDINERQEQEDYQEMLQSLAQRPAPANTNRERRPRYQHPKGAPNADLIFKTGGR

Target ORF information:

RefSeq Version	XM_012958641.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis UPF2 regulator of nonsense transcripts homolog (upf2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012958641.2

ORF Insert Sequence:

ATGCCAGCAG AACGCAAGAA ACCAGCAGGT ATGGAAGAAA AGGAATCTCC ATCTAGCCCA 
AGGGACAAAG AATGTGCTGA GAAGAAATCT ACAAGCAGCA AAGATCGGGT TAAGGAAGAA 
CCAAAAGCAA GCACCAAAAG AGATCAATCA AAGGCTTCTG AGGAAAAAAA GAAGCGAACT 
GATGAGGACA AGCGGAAAAA AGAGGAGAAG GAGTGCAAAA GAAAGGAGGA TGATCAAGTC 
AAAGCAGATG ATGAGCAGCA AAGTGCAGAG GGAAAAAATC AAGAAGATGA AATAAAAAAG 
AAGCAGGAAG AAGAGGAAGC TCATCAGAGA GAAGCTGCAG CATTGCAAAT CAAAGAGAAG 
GATGAAGCAA ATCAGTTACA CCAAGAAGCA TGGGAACGGC ACCACACTAG GAAGGAGCTT 
CGTAGCAAAA ATCAAAATGC CCCTGAAAAT CGACCTGAGG AGAGTTTCTT TAGCAGGCTG 
GACTCTAGTT TGAAGAAGAA CACTGCTTTT GTGAAGAAAC TCAAAACCAT TACTGAACAG 
CAAAGAGATT CATTGTCCCA TGACTTCAAC AGCCTTAACC TCAGCAAGTA CATAGCAGAG 
GCAGCAGCCT CCATTGTAGA GGCCAAGTTA AAAATATCTG ATGTTAACTG TGCTGTGCAT 
CTGTGTTCTC TTTTCCACCA GCGATATGCT GACTTTGCAC AGTCTCTCCT TCAGGTATGG 
AAGAAGCATT TTGAAGCCCG CAAAGAGGAA AAGACTCCTA ATATTACTAA GCTGAGAACA 
GACTTGCGCT TTATTGCAGA GCTAACCATC ATTGGCATCT TTACTGATAA AGAAGGCCTC 
TCTCTTATTT ATGAGCAACT TAAAAACATA ATTAATGCTG ATCGAGAATC CCACACACAT 
GTTTCAGTAG TAATCAGTTT TTGCCGGCAC TGTGGAGATG ATATTGCAGG CTTAGTGCCC 
AGGAAAGTGA AGTGTGCAGC TGAAAAATAC AATCTTAATT TTCCTCCTAG TGAGATTATC 
AGCCCAGAGA AACAACAGCC TTTCCAGAAT TTGCTGAAAG AGTATTTCAC ATCTCTGACG 
AAACACTTGA AGAGGGACCA CAGAGAATTA CAAAATATAG AGCGCCAGAA CAGGCGCATT 
CTTCACACTA AAGGGGAGCT AAGTGAAGAC AGGCACAAGC AATATGAGGA GTTTGCAACA 
TCTTATCAGA AGCTGTTAGC TAGCTGTCAA TCACTTGCAG ATCTGCTAGA TGAAAATATG 
CCAGAACTTC CTCAGGACAA ACCTGCTCCG GAGGAACATG GGCCTGGTAT TGATATCTTC 
ACACCTGGAA AACCTGGAGA TTATGACTTA GAAGGAGGCA TTTGGGAAGA CGAGGATGCC 
CGTAACTTTT ATGAGAATCT GATTGATTTG AAAGCTTTTG TCCCAGCCAT TTTATTTAAG 
GATAATGAGA AAAATTCTCA GAGTAAAGAA ACTGGCAAAG ACAGTTTCAA AGATAGTAAA 
GAGAAAGATG TGTCCACAAC TGAAGAGCTA GAGCTGGAGT TAGAGACATT GGATATTAAT 
GATGAAGGCA TGGAACTGGA AGGTGCCGAA GATGCAGAAG ACCTAACAAA AAAACTTTTG 
GATGAACAAG AGCAAGAAGA TGAAGAGGCA AGCACAGGCT CCCATTTAAA ACTCATTGTT 
GATGCTTTTA TTCAGCAGCT GCCAAACTGT GTTAATCGTG ATCTAATAGA TAAGGCTGCA 
ATGGATTTTT GCATGAACAT GAATACAAAA GCTAATAGAA GGAAATTGGT CAGAGCACTA 
TTCATTGTTC CCAGACAAAG ACTGGATCTT CTTCCATTCT ATGCCAGATT AGTGGCCACC 
TTGCATCCTT GCATGTCTGA TGTTGCAGAA GACCTTTGTT CCATGTTAAA GGGAGACTTT 
CGTTTCCACG TTAGAAAGAA GGATCAAATC AACATTGAAA CAAAGAACAA GACTGTGAGA 
TTTATTGGAG AACTTGCCAA ATTTAAAATG TTCAGCAAAA CAGATACGCT GCATTGTTTA 
AAGATGCTGT TGTCAGACTT TTCGCATCAC CACATAGAAA TGGCATGCAC TCTACTGGAA 
ACTTGTGGCA GATTTCTTTT TCGATCTCCA GATTCTCACT TGCGCACCAG TGTCCTGCTG 
GAGCAAATGA TGCGCAAGAA GCAAGCAATG CACTTAGATG CACGCTATGT TACAATGGTT 
GAAAATGCCT ACTATTACTG TAACCCACCT CCAGCTGAAA AAACTGTGAA AAAGAAGCGA 
CCTCCACTAC AAGAATACAT CAGAAAGCTT CTTTATAAAG ACCTTTCCAA GGTCACCACT 
GAAAAGGTTT TGAGGCAGAT GAGGAAGCTG TCTTGGCAGG ATCCTGAAGT GAAGGACTAT 
GTAATTTGCT GCATGATCAA CATCTGGAAT GTTAAATACA ACAGCATTCA TTGTGTAGCC 
AATCTTTTGG CAGGACTGGT ACTTTACCAA GAGGATGTTG GAATACATGT TGTAGATGGA 
GTGTTAGAAG ATATACGTCT TGGGATGGAG GTTAACCAGC CAAAATTTAA TCAGCGGAGG 
ATAAGCAGTG CAAAGTTCCT TGGGGAGCTT TACAACTATA GAATGGTGGA ATCTGCAGTT 
ATATTTCGCA CACTTTATTC ATTTATTTCC TTTGGTGTAA ATTCTGATGG AAGCCCCAGC 
CCACTTGATC CTCCTGAACA CTTGTTCCGC ATTCGATTAG TTTGCACTAT CCTTGACACT 
TGTGGCCAGT ATTTTGACCG TGGATCAAGC AAGAGAAAGC TTGATTGTTT TCTTGTTTAT 
TTTCAGCGCT ACGTTTGGTG GAAGAAAAGT CTTGATGTGT GGACTAAAGA CCACCCATTT 
CCAATAGATA TTGATTATAT GATAAGTGAT ACCTTGGAGC TTCTGAGGCC AAAAATGAAG 
CTCTGTGCCT CACTGAATGA ATCTGTCCGC CAAGTGCAGG ATTTGGAGCG TGAGTTTCTG 
ACAAAGCTAG GTCTCATGAA TGATAAGGAA TCCAAACATT CCATGACAGA AGGAGATCAC 
CTAGTGTATG AAGATGATGA TGAGGAAGAA GAGGCTGGGG CAGAGACAGA GGAGCAGTCT 
GGCAATGAAA GTGAAATGAA TGAACAAGAA GAAGATGCTT CTGAAAATGA AGAGGATGAA 
AGAGAGGAGG AAGAAGAGGA GAATACAGAT TATATGACTG ACTCAAACAA AGAAAATGAA 
ACAGATGAAG AAAACACTGA GGTGATGATT AAAGGAGGTG GAATTAAACA TGTACCCTGT 
GCTGAAGATG AAGACTTTAT TCAAGCCCTT GACAAGATGA TGCTGGAAAA TCTTCAGCAA 
CGTAGTGGAG AGTCAGTGAA GGTGCATCAG TTAGATGTTG CTATTCCCCT GCATCTTAAG 
AGCCAGCTTA AAAAGGGACC AGCTATGAGC ACTGGAGAAG TAGACCCGGA ATCTGGCGAC 
ACTATGCCAT TTGTTATGCT TACAAGAAGA GGTAACAAAC AGCAGTTCAA GATCCTTAAT 
GTGCCTATGT CCTCCCAACT GGCTGCAAAT CACTGGAGTC AGCAGCAAGC TGAACAAGAA 
GAGAGGATGA GGATGAAGAA ACTCACATTA GATATCAATG AGCGTCAGGA ACAAGAAGAT 
TACCAAGAAA TGTTGCAGTC ATTGGCTCAG CGCCCAGCAC CTGCCAACAC AAACAGGGAG 
CGCAGGCCTC GATACCAGCA TCCAAAGGGA GCACCTAACG CAGACCTCAT CTTCAAAACT 
GGGGGCAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

upf2 ( XM_012958641.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012814095.2 Xenopus tropicalis UPF2, regulator of nonsense mediated mRNA decay (upf2), transcript variant X2, mRNA.

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CloneID	OXa53297
Clone ID Related Accession (Same CDS sequence)	XM_012958641.3
Accession Version	XM_012958641.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	regulator of nonsense transcripts 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012958641.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGCAG AACGCAAGAA ACCAGCAGGT ATGGAAGAAA AGGAATCTCC ATCTAGCCCA 
AGGGACAAAG AATGTGCTGA GAAGAAATCT ACAAGCAGCA AAGATCGGGT TAAGGAAGAA 
CCAAAAGCAA GCACCAAAAG AGATCAATCA AAGGCTTCTG AGGAAAAAAA GAAGCGAACT 
GATGAGGACA AGCGGAAAAA AGAGGAGAAG GAGTGCAAAA GAAAGGAGGA TGATCAAGTC 
AAAGCAGATG ATGAGCAGCA AAGTTCAGAG GGAAAAAATC AAGAAGATGA AATAAAAAAG 
AAGCAGGAAG AAGAGGAAGC TCATCAGAGA GAAGCTGCAG CATTACACAT CAAAGAGAAG 
GATGAAGCAA ATCAGTTACA CCAAGAAGCA TGGGAACGGC ACCACACTAG GAAGGAGCTT 
CGTAGCAAAA ATCAAAATGC CCCTGAAAAT CGACCTGAGG AGAGTTTCTT TAGCAGGCTG 
GACTCTAGTT TGAAGAAGAA CACTGCTTTT GTGAAGAAAC TCAAAACCAT TACTGAACAG 
CAAAGAGATT CATTGTCCCA TGACTTCAAC AGCCTTAACC TCAGCAAGTA CATAGCAGAG 
GCAGCAGCCT CCATTGTAGA GGCCAAGTTA AAAATATCTG ATGTTAACTG TGCTGTGCAT 
CTGTGTTCTC TTTTCCACCA GCGATATGCT GACTTTGCAC AGTCTCTCCT TCAGGTATGG 
AAGAAGCATT TTGAAGCCCG CAAAGAGGAA AAGACTCCTA ATATTACTAA GCTGAGAACA 
GACTTGCGCT TTATTGCAGA GCTAACCATC ATTGGCATCT TTACTGATAA AGAAGGCCTC 
TCTCTAATTT ATGAGCAACT TAAAAACATA ATTAATGCTG ATCGAGAATC CCACACACAT 
GTTTCAGTAG TAATCAGTTT TTGCCGGCAC TGTGGAGATG ATATTGCAGG CTTAGTGCCC 
AGGAAAGTGA AGTGTGCAGC TGAAAAATAC AATCTTAATT TTCCTCCTAG TGAGATTATC 
AGCCCAGAGA AACAACAGCC TTTCCAGAAT TTGCTGAAAG AGTATTTCAC ATCTCTGACG 
AAACACTTGA AGAGGGACCA CAGAGAATTA CAAAATATAG AGCGCCAGAA CAGGCGCATT 
CTTCACACTA AAGGGGAGCT AAGTGAAGAC AGGCACAAGC AATATGAGGA GTTTGCAACA 
TCTTATCAGA AGCTGTTAGC TAGCTGTCAA TCACTTGCAG ATCTGCTAGA TGAAAATATG 
CCAGAACTTC CTCAGGACAA ACCTGCTCCG GAGGAACATG GGCCTGGTAT TGATATCTTC 
ACACCTGGAA AACCTGGAGA TTATGACTTA GAAGGAGGCA TTTGGGAAGA CGAGGATGCC 
CGTAACTTTT ATGAGAATCT GATTGATTTG AAAGCTTTTG TCCCAGCCAT TTTATTTAAG 
GATAATGAGA AAAATTCTCA GAGTAAAGAA ACTGGCAAAG ACAGTTTCAA AGATAGTAAA 
GAGAAAGATG TGTCCACAAC TGAAGAGCTA GAGCTGGAGT TAGAGACATT GGATATTAAT 
GATGAAGGCA TGGAACTGGA AGGTGCCGAA GATGCAGAAG ACCTAACAAA AAAACTTTTG 
GATGAACAAG AGCAAGAAGA TGAAGAGGCA AGCACAGGCT CCCATTTAAA ACTCATTGTT 
GATGCTTTTA TTCAGCAGCT GCCAAACTGT GTTAATCGTG ATCTAATAGA TAAGGCTGCA 
ATGGATTTTT GCATGAACAT GAATACAAAA GCTAATAGAA GGAAATTGGT CAGAGCACTA 
TTCATTGTTC CCAGACAAAG ACTGGATCTT CTTCCATTCT ATGCCAGATT AGTGGCCACC 
TTGCATCCTT GCATGTCTGA TGTTGCAGAA GACCTTTGTT CCATGTTAAA GGGAGACTTT 
CGTTTCCACG TTAGAAAGAA GGATCAAATC AACATTGAAA CAAAGAACAA GACTGTGAGA 
TTTATTGGAG AACTTGCCAA ATTTAAAATG TTCAGCAAAA CAGATACGCT GCATTGTTTA 
AAGATGCTGT TGTCAGACTT TTCGCATCAC CACATAGAAA TGGCATGCAC TCTACTGGAA 
ACTTGTGGCA GATTTCTTTT TCGATCTCCA GATTCTCACT TGCGCACCAG TGTCCTGCTG 
GAGCAAATGA TGCGCAAGAA GCAAGCAATG CACTTAGATG CACGCTATGT TACAATGGTT 
GAAAATGCCT ACTATTACTG TAACCCACCT CCAGCTGAAA AAACTGTGAA AAAGAAGCGA 
CCTCCACTAC AAGAATACAT CAGAAAGCTT CTTTATAAAG ACCTTTCCAA GGTCACCACT 
GAAAAGGTTT TGAGGCAGAT GAGGAAGCTG TCTTGGCAGG ATCCTGAAGT GAAGGACTAT 
GTAATTTGCT GCATGATCAA CATCTGGAAT GTTAAATACA ACAGCATTCA TTGTGTAGCC 
AATCTTTTGG CAGGACTGGT ACTTTACCAA GAGGATGTTG GAATACATGT TGTAGATGGA 
GTGTTAGAAG ATATACGTCT TGGGATGGAG GTTAACCAGC CAAAATTTAA TCAGCGGAGG 
ATAAGCAGTG CAAAGTTCCT TGGGGAGCTT TACAACTATA GAATGGTGGA ATCTGCAGTT 
ATATTTCGCA CACTTTATTC ATTTATTTCC TTTGGTGTAA ATTCTGATGG AAGCCCCAGC 
CCACTTGATC CTCCTGAACA CTTGTTCCGC ATTCGATTAG TTTGCACTAT CCTTGACACT 
TGTGGCCAGT ATTTTGACCG TGGATCAAGC AAGAGAAAGC TTGATTGTTT TCTTGTTTAT 
TTTCAGCGCT ACGTTTGGTG GAAGAAAAGT CTTGATGTGT GGACTAAAGA CCACCCATTT 
CCAATAGATA TTGATTATAT GATAAGTGAT ACCTTGGAGC TTCTGAGGCC AAAAATGAAG 
CTCTGTGCCT CACTGAATGA ATCTGTCCGC CAAGTGCAGG ATTTGGAGCG TGAGTTTCTG 
ACAAAGCTAG GTCTCATGAA TGATAAGGAA TCCAAACATT CCATGACAGA AGGAGATCAC 
CTAGTGTATG AAGATGATGA TGAGGAAGAA GAGGCTGGGG CAGAGACAGA GGAGCAGTCT 
GGCAATGAAA GTGAAATGAA TGAACAAGAA GAAGATGCTT CTGAAAATGA AGAGGATGAA 
AGAGAGGAGG AAGAAGAGGA GAATACAGAT TATATGACTG ACTCAAACAA AGAAAATGAA 
ACAGATGAAG AAAACACTGA GGTGATGATT AAAGGAGGTG GAATTAAACA TGTACCCTGT 
GCTGAAGATG AAGACTTTAT TCAAGCCCTT GACAAGATGA TGCTGGAAAA TCTTCAGCAA 
CGTAGTGGAG AGTCAGTGAA GGTGCATCAG TTAGATGTTG CTATTCCCCT GCATCTTAAG 
AGCCAGCTTA AAAAGGGACC AGCTATGAGC ACTGGAGAAG TAGACCCGGA ATCTGGCGAC 
ACTATGCCAT TTGTTATGCT TACAAGAAGA GGTAACAAAC AGCAGTTCAA GATCCTTAAT 
GTGCCTATGT CCTCCCAACT GGCTGCAAAT CACTGGAGTC AGCAGCAAGC TGAACAAGAA 
GAGAGGATGA GGATGAAGAA ACTCACATTA GATATCAATG AGCGTCAGGA ACAAGAAGAT 
TACCAAGAAA TGTTGCAGTC ATTGGCTCAG CGCCCAGCAC CTGCCAACAC AAACAGGGAG 
CGCAGGCCTC GATACCAGCA TCCAAAGGGA GCACCTAACG CAGACCTCAT CTTCAAAACT 
GGGGGCAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012814095.2
CDS	286..4077
Translation	MPAERKKPAGMEEKESPSSPRDKECAEKKSTSSKDRVKEEPKASTKRDQSKASEEKKKRTDEDKRKKEEKECKRKEDDQVKADDEQQSSEGKNQEDEIKKKQEEEEAHQREAAALHIKEKDEANQLHQEAWERHHTRKELRSKNQNAPENRPEESFFSRLDSSLKKNTAFVKKLKTITEQQRDSLSHDFNSLNLSKYIAEAAASIVEAKLKISDVNCAVHLCSLFHQRYADFAQSLLQVWKKHFEARKEEKTPNITKLRTDLRFIAELTIIGIFTDKEGLSLIYEQLKNIINADRESHTHVSVVISFCRHCGDDIAGLVPRKVKCAAEKYNLNFPPSEIISPEKQQPFQNLLKEYFTSLTKHLKRDHRELQNIERQNRRILHTKGELSEDRHKQYEEFATSYQKLLASCQSLADLLDENMPELPQDKPAPEEHGPGIDIFTPGKPGDYDLEGGIWEDEDARNFYENLIDLKAFVPAILFKDNEKNSQSKETGKDSFKDSKEKDVSTTEELELELETLDINDEGMELEGAEDAEDLTKKLLDEQEQEDEEASTGSHLKLIVDAFIQQLPNCVNRDLIDKAAMDFCMNMNTKANRRKLVRALFIVPRQRLDLLPFYARLVATLHPCMSDVAEDLCSMLKGDFRFHVRKKDQINIETKNKTVRFIGELAKFKMFSKTDTLHCLKMLLSDFSHHHIEMACTLLETCGRFLFRSPDSHLRTSVLLEQMMRKKQAMHLDARYVTMVENAYYYCNPPPAEKTVKKKRPPLQEYIRKLLYKDLSKVTTEKVLRQMRKLSWQDPEVKDYVICCMINIWNVKYNSIHCVANLLAGLVLYQEDVGIHVVDGVLEDIRLGMEVNQPKFNQRRISSAKFLGELYNYRMVESAVIFRTLYSFISFGVNSDGSPSPLDPPEHLFRIRLVCTILDTCGQYFDRGSSKRKLDCFLVYFQRYVWWKKSLDVWTKDHPFPIDIDYMISDTLELLRPKMKLCASLNESVRQVQDLEREFLTKLGLMNDKESKHSMTEGDHLVYEDDDEEEEAGAETEEQSGNESEMNEQEEDASENEEDEREEEEEENTDYMTDSNKENETDEENTEVMIKGGGIKHVPCAEDEDFIQALDKMMLENLQQRSGESVKVHQLDVAIPLHLKSQLKKGPAMSTGEVDPESGDTMPFVMLTRRGNKQQFKILNVPMSSQLAANHWSQQQAEQEERMRMKKLTLDINERQEQEDYQEMLQSLAQRPAPANTNRERRPRYQHPKGAPNADLIFKTGGR

Target ORF information:

RefSeq Version	XM_012958641.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis UPF2, regulator of nonsense mediated mRNA decay (upf2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012958641.3

ORF Insert Sequence:

ATGCCAGCAG AACGCAAGAA ACCAGCAGGT ATGGAAGAAA AGGAATCTCC ATCTAGCCCA 
AGGGACAAAG AATGTGCTGA GAAGAAATCT ACAAGCAGCA AAGATCGGGT TAAGGAAGAA 
CCAAAAGCAA GCACCAAAAG AGATCAATCA AAGGCTTCTG AGGAAAAAAA GAAGCGAACT 
GATGAGGACA AGCGGAAAAA AGAGGAGAAG GAGTGCAAAA GAAAGGAGGA TGATCAAGTC 
AAAGCAGATG ATGAGCAGCA AAGTTCAGAG GGAAAAAATC AAGAAGATGA AATAAAAAAG 
AAGCAGGAAG AAGAGGAAGC TCATCAGAGA GAAGCTGCAG CATTACACAT CAAAGAGAAG 
GATGAAGCAA ATCAGTTACA CCAAGAAGCA TGGGAACGGC ACCACACTAG GAAGGAGCTT 
CGTAGCAAAA ATCAAAATGC CCCTGAAAAT CGACCTGAGG AGAGTTTCTT TAGCAGGCTG 
GACTCTAGTT TGAAGAAGAA CACTGCTTTT GTGAAGAAAC TCAAAACCAT TACTGAACAG 
CAAAGAGATT CATTGTCCCA TGACTTCAAC AGCCTTAACC TCAGCAAGTA CATAGCAGAG 
GCAGCAGCCT CCATTGTAGA GGCCAAGTTA AAAATATCTG ATGTTAACTG TGCTGTGCAT 
CTGTGTTCTC TTTTCCACCA GCGATATGCT GACTTTGCAC AGTCTCTCCT TCAGGTATGG 
AAGAAGCATT TTGAAGCCCG CAAAGAGGAA AAGACTCCTA ATATTACTAA GCTGAGAACA 
GACTTGCGCT TTATTGCAGA GCTAACCATC ATTGGCATCT TTACTGATAA AGAAGGCCTC 
TCTCTAATTT ATGAGCAACT TAAAAACATA ATTAATGCTG ATCGAGAATC CCACACACAT 
GTTTCAGTAG TAATCAGTTT TTGCCGGCAC TGTGGAGATG ATATTGCAGG CTTAGTGCCC 
AGGAAAGTGA AGTGTGCAGC TGAAAAATAC AATCTTAATT TTCCTCCTAG TGAGATTATC 
AGCCCAGAGA AACAACAGCC TTTCCAGAAT TTGCTGAAAG AGTATTTCAC ATCTCTGACG 
AAACACTTGA AGAGGGACCA CAGAGAATTA CAAAATATAG AGCGCCAGAA CAGGCGCATT 
CTTCACACTA AAGGGGAGCT AAGTGAAGAC AGGCACAAGC AATATGAGGA GTTTGCAACA 
TCTTATCAGA AGCTGTTAGC TAGCTGTCAA TCACTTGCAG ATCTGCTAGA TGAAAATATG 
CCAGAACTTC CTCAGGACAA ACCTGCTCCG GAGGAACATG GGCCTGGTAT TGATATCTTC 
ACACCTGGAA AACCTGGAGA TTATGACTTA GAAGGAGGCA TTTGGGAAGA CGAGGATGCC 
CGTAACTTTT ATGAGAATCT GATTGATTTG AAAGCTTTTG TCCCAGCCAT TTTATTTAAG 
GATAATGAGA AAAATTCTCA GAGTAAAGAA ACTGGCAAAG ACAGTTTCAA AGATAGTAAA 
GAGAAAGATG TGTCCACAAC TGAAGAGCTA GAGCTGGAGT TAGAGACATT GGATATTAAT 
GATGAAGGCA TGGAACTGGA AGGTGCCGAA GATGCAGAAG ACCTAACAAA AAAACTTTTG 
GATGAACAAG AGCAAGAAGA TGAAGAGGCA AGCACAGGCT CCCATTTAAA ACTCATTGTT 
GATGCTTTTA TTCAGCAGCT GCCAAACTGT GTTAATCGTG ATCTAATAGA TAAGGCTGCA 
ATGGATTTTT GCATGAACAT GAATACAAAA GCTAATAGAA GGAAATTGGT CAGAGCACTA 
TTCATTGTTC CCAGACAAAG ACTGGATCTT CTTCCATTCT ATGCCAGATT AGTGGCCACC 
TTGCATCCTT GCATGTCTGA TGTTGCAGAA GACCTTTGTT CCATGTTAAA GGGAGACTTT 
CGTTTCCACG TTAGAAAGAA GGATCAAATC AACATTGAAA CAAAGAACAA GACTGTGAGA 
TTTATTGGAG AACTTGCCAA ATTTAAAATG TTCAGCAAAA CAGATACGCT GCATTGTTTA 
AAGATGCTGT TGTCAGACTT TTCGCATCAC CACATAGAAA TGGCATGCAC TCTACTGGAA 
ACTTGTGGCA GATTTCTTTT TCGATCTCCA GATTCTCACT TGCGCACCAG TGTCCTGCTG 
GAGCAAATGA TGCGCAAGAA GCAAGCAATG CACTTAGATG CACGCTATGT TACAATGGTT 
GAAAATGCCT ACTATTACTG TAACCCACCT CCAGCTGAAA AAACTGTGAA AAAGAAGCGA 
CCTCCACTAC AAGAATACAT CAGAAAGCTT CTTTATAAAG ACCTTTCCAA GGTCACCACT 
GAAAAGGTTT TGAGGCAGAT GAGGAAGCTG TCTTGGCAGG ATCCTGAAGT GAAGGACTAT 
GTAATTTGCT GCATGATCAA CATCTGGAAT GTTAAATACA ACAGCATTCA TTGTGTAGCC 
AATCTTTTGG CAGGACTGGT ACTTTACCAA GAGGATGTTG GAATACATGT TGTAGATGGA 
GTGTTAGAAG ATATACGTCT TGGGATGGAG GTTAACCAGC CAAAATTTAA TCAGCGGAGG 
ATAAGCAGTG CAAAGTTCCT TGGGGAGCTT TACAACTATA GAATGGTGGA ATCTGCAGTT 
ATATTTCGCA CACTTTATTC ATTTATTTCC TTTGGTGTAA ATTCTGATGG AAGCCCCAGC 
CCACTTGATC CTCCTGAACA CTTGTTCCGC ATTCGATTAG TTTGCACTAT CCTTGACACT 
TGTGGCCAGT ATTTTGACCG TGGATCAAGC AAGAGAAAGC TTGATTGTTT TCTTGTTTAT 
TTTCAGCGCT ACGTTTGGTG GAAGAAAAGT CTTGATGTGT GGACTAAAGA CCACCCATTT 
CCAATAGATA TTGATTATAT GATAAGTGAT ACCTTGGAGC TTCTGAGGCC AAAAATGAAG 
CTCTGTGCCT CACTGAATGA ATCTGTCCGC CAAGTGCAGG ATTTGGAGCG TGAGTTTCTG 
ACAAAGCTAG GTCTCATGAA TGATAAGGAA TCCAAACATT CCATGACAGA AGGAGATCAC 
CTAGTGTATG AAGATGATGA TGAGGAAGAA GAGGCTGGGG CAGAGACAGA GGAGCAGTCT 
GGCAATGAAA GTGAAATGAA TGAACAAGAA GAAGATGCTT CTGAAAATGA AGAGGATGAA 
AGAGAGGAGG AAGAAGAGGA GAATACAGAT TATATGACTG ACTCAAACAA AGAAAATGAA 
ACAGATGAAG AAAACACTGA GGTGATGATT AAAGGAGGTG GAATTAAACA TGTACCCTGT 
GCTGAAGATG AAGACTTTAT TCAAGCCCTT GACAAGATGA TGCTGGAAAA TCTTCAGCAA 
CGTAGTGGAG AGTCAGTGAA GGTGCATCAG TTAGATGTTG CTATTCCCCT GCATCTTAAG 
AGCCAGCTTA AAAAGGGACC AGCTATGAGC ACTGGAGAAG TAGACCCGGA ATCTGGCGAC 
ACTATGCCAT TTGTTATGCT TACAAGAAGA GGTAACAAAC AGCAGTTCAA GATCCTTAAT 
GTGCCTATGT CCTCCCAACT GGCTGCAAAT CACTGGAGTC AGCAGCAAGC TGAACAAGAA 
GAGAGGATGA GGATGAAGAA ACTCACATTA GATATCAATG AGCGTCAGGA ACAAGAAGAT 
TACCAAGAAA TGTTGCAGTC ATTGGCTCAG CGCCCAGCAC CTGCCAACAC AAACAGGGAG 
CGCAGGCCTC GATACCAGCA TCCAAAGGGA GCACCTAACG CAGACCTCAT CTTCAAAACT 
GGGGGCAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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upf2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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