cenpf cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	cenpf
Entrez Gene ID	100127686
Full Name	centromere protein F
General protein information	Preferred Names centromere protein F Names centromere protein F centromere protein F, 350/400kDa (mitosin) centromere protein F, 350/400ka (mitosin)
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002934692.4	XP_002934738.2	centromere protein F
XM_002934692.5	XP_002934738.2	centromere protein F

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	CENPF	XP_001105956.2
Xenopus tropicalis (tropical clawed frog)	cenpf	XP_002934738.2
Homo sapiens (human)	CENPF	NP_057427.3
Pan troglodytes (chimpanzee)	CENPF	XP_001171564.1
Canis lupus familiaris (dog)	CENPF	XP_003639228.1
Mus musculus (house mouse)	Cenpf	NP_001074832.2
Bos taurus (cattle)	CENPF	NP_001243515.1
Gallus gallus (chicken)	CENPF	NP_989976.2
Rattus norvegicus (Norway rat)	Cenpf	NP_001094297.1

Gene Ontology

The following cenpf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cenpf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa10912	XM_002934692.5 Latest version!	Xenopus tropicalis centromere protein F (cenpf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
View Old Accession Versions >>
OXa10912	XM_002934692.4	Xenopus tropicalis centromere protein F (cenpf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cenpf ( XM_002934692.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934738.2 Xenopus tropicalis centromere protein F (cenpf), mRNA.

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CloneID	OXa10912
Clone ID Related Accession (Same CDS sequence)	XM_002934692.4 , XM_002934692.5
Accession Version	XM_002934692.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	centromere protein F
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002934692.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTGGG TCGTAGAGGA GTGGAAGGAG GGGCTGCCCA CAAAGACCCT TCAGAAGATC 
CAGGAGCTGG AAAGTCAGTT GGACAAACTA AAGAAAGAGC GGCAGCAAAG GCAGTTTCAG 
CTGGAGTCGC TGGAAGCCGC TTTCCAAAAG CAGAAGCAAA AGGCAGAAAG TGAAAAAAGC 
GAAATCTCCG CCCTAAAACG AGAGAATCAG AGTTTGATAG AATCATGTGA CAATCAGGAG 
AAGGCAAAGC AGAAGATTAC CCATGAGTAC CAGCTAAAAG AGTCACAGGT CAACTTCCTG 
GAGGGCCAAC TCAGCGCCAG CAAGAAGCAA ATCGAAAAAC TGGAGCAAGA GCTTAAAAGG 
TACAAGAGTG ACTTGGAGAA AAGCCAACTG TCGTTCAATG CCTGTGACAT GTCTGTGTGC 
GCCACTCCCC AGAAGAGCTT TGTGGCGCCA TATACACCCA ATAAATACAC TGATTTCAGG 
TATGAAGAAT TGCAAGAGAA ATATCAGAAA GAGGTGGAAG AGAGGAAGAG GTTGGAAACA 
GAGATGAAAA CATTAAGGAA TGTGCCCCAG CCTCCACAGG CCGCTGCCCA GAGCACCATG 
ACGCACCGGG ATATCGCGCG GCACCAGACT TCCTCGTCTG TGTTCTCGTG GCAAGAAGAT 
CGAACCCCAA GTCACCCCTC ATCTACCAGT CATGATAAAA GACTTAAGAG AAGTTTTGCA 
TCATCCCAGC TCCTGTGGGA CCAGGAGGAA ACTCCAAGCA AAAGAGGTGT CCGATCAGAG 
AACTTGGGTA AAAGTGCCAG TGACTCCTAC ACCACTCAGC TTAATGATCA GCTAAAAACC 
CAGAATCAAG AGCTGAAATC CAGAATAAAT GAGCTGGAGA TGCGCTTGCA AGTTCAAGAG 
AAAGATCTGA AAAATCAGCT TAACAAACTC CAGGAAAGCC AAGCTCTGTA TGAAAAAACC 
CAAAGGGAAC TCGCAGAGAG AGACAAAACC CTGGTGAAAT GCAAGGAGGA CTTGGCACGT 
GTGACTGCGC AGTGTGAGCA GGCTGCTAAT AAGTGTGTGA TGGCGGAACA GAAGTTAAAG 
AAAGTTTCAG ATGAGCTGAG CTGCCAGAGG CAAAATGCTG AGAGTGCGAG GGTTGCTGCG 
GAGCAGAAAC TAAAGGAACG AGAGAAGGAA AACCAACAGG AGATTTTACA GCAGCAGAAT 
TCCCTAAGGA ATATGGAGCA GCAGCTCAGC CAGATAAAAG CCAAGATGAG CCAGGAGGCA 
CAGCAAGCCA AAAATGACTT CAACGCCCTC CAATCAGAGC TTGACCGGGC CATTCACACG 
AAAAAGATTT TGGAAAACGA AACAGAAGAA CTAAGGCAGA GGCTCAGCCG AGCGGAACAG 
GACTTGTGTC AGTGCCGAAG TAACGAAAAT GATTTAAGGA AGAGCCTTGA GGACACCAAG 
TCGGAAAAAA ATGTAATGAA AAGCCAGTAT GATCAGAAAT CTAAAGACGC TCTGAAACTG 
GAAGAAGAAT TACAAACGGC GAACCAAACC CTGAGGCAGA ACCAGCTCTT TGTGGATGAA 
ATAAAAGCTA AGAATCTTCA TTTAGAAGCA GAACTGAAGT CAGCTTTTGA GAAACTGCAA 
GACCACGACT CTGCCACCTT CCTGAATTTG AAAGCCACGG TGCATAATTT GGAAATGGAG 
AGGAATTTTG CCCAAGAATT ATTAAAGAAA AGAGAGAATG ATTTTGAAGA AATTAAAAAT 
AATTTAGCGA GAATGATGGA TGAAACAAAC TCATTAAAAA GTCACCTTGA TCACAAAGAA 
AAAGAATGTA AGGAACTGAT AAATGCTAAT ATTTCACTTA ATAGCTGGAA AGTGGAACAG 
GAGAAGGTGG TGGGTCAAAT TAGCAGAGAT AAAGATGATC TGCTGAAGAA GATTCAGGAG 
TTGGAACATT TGCTGCAGAC TCACGGTGAC CAAGTACACT TGTTAGAAAA TGACAAGAAA 
AACTTGCACA CTCAAATTAG AACCTTGCAA GAGATCGTCG ATGCCAAGAC CGTGGACCTG 
CAAAATCAGC AACTGGTATG CGGTGAACTT CGACAAAATC TGGACTACGA AAACCAGAAA 
TTTAATAGCG AAAATGAAAA GTTGCAGCTA CAAATTTCCG AGCTTGAGAA TGCAATCAAA 
GAGCAAAATT GTTCCGCTAG TTTGGACAAA TCGTCTTATT GCGAAAACTT GCTATTCCAG 
GAGAAGCAAT TGAATTCCGA AATGCAAAAA CAGATGAAAG AACTGTTATT AGAGAAGGAA 
GATATCCAGA AATGTCTAAA TGAAGCAGAG GTATTGCACG AACAGTTTGT CACAGGCTCC 
CGAAGCCACA TGGAGAGCTT GCAGGAAAAC ATTTCTTCCA AGCAGAACTA CATTGAGTCA 
ATAGAATGTG CAGTTAGAGA GAAAGAAGAG CAAATTACCA CTTTAACCGA AAAATTAGGA 
GTTCTAGATG CAAACTTGCA ATCTGTCCAG GAGAAAAACA AAGAGCTTAA TGATAAACTG 
CAGGAGCTCA ACCTGCTTTC TGAATCGTGG CCTTTAGAGA GAGAAAGACT AAATTCCCAG 
ATTAGTTTGA ATCGGGAGGA AATTGAACGG CTGACTGAAG AAAATAAAAG AACCCATCAA 
GTCAATGATC CTCACAATGA TCAGGAAACA AACAAAGAAT CGGAGCACCA GTTAGAGACT 
ACCCAGCCAT CGGCAGAAGA TAAAGTTGAG CCTAGAGGGT TGCCTGATGG GGCGGGTATT 
GAGAATACCA ATGAGCTGGA AGCACTTCAG CAAGAACTCG TTCATGTAAA AGAAAAACAC 
TGTAGACTGC AAAGAGAATA TGACAGACTC ACTAATGCCA ACGAGGAACT TACCAAGCTG 
CTTGATGACC TCCGAAACAA CGAACAGTCT CTCGATAACA TCACCGATTT AAGTGCTTAT 
TTGAAGGAAA AGGAAAATTC TCTAAATAAC AGTAATTTTG TGCTTGAGAC TCTTCCAATG 
GATCTTGATG ATGAGAAACA AATTCACGAT GGATGCACTG ATTCTCCTAC ACTAAAGGGT 
ACACCAAACA CCGTGAAGTC CAACAATGAT CTAGATTTAG AACAAGAGCT ATCTACACTT 
CAAAAACAGG ACATATTTTC ATTTGATATC GACCAGGCAG AACGTGCCAC CAATATTTCA 
GACCTTCTGG TGAACATCAG TGACCATCAA ATAGGTGATC GTCCATCCAG TAGTCTTGAT 
TCCAGCCAGT GCGATGACAG TATTAACCTT AATAAAGACA TTTTAGGCCT TCGAATATCT 
GGTGGTTGGG CAACTACAAA ACCACCTCAG TCTATTAGTG ACAACGAAAA TAGTGGTCTC 
TTGACTTCAC CGAACGAGGG CACATCAAAA CGTTTGAAAG AGACTGCAGA AAACATATTG 
AAATCACCAG TTCCTCCAAG TCAGCGTAGT AGTCCATCCT CAATGAATTC CAATAACAGA 
GGAGGCCGTG AAGCCAATAG AAAGTCTATC CCTTGCAAAT CTGCTGCTGA CTCCATTCTT 
GGGAAATCAC TCGAAGATCA GTTATGGATG ATGGCTGTTA TGGCCAGCGG AGATGCCGGA 
GACTTTAAAG AATTTCTTAC AACTTACCAA ACAGAAGTGG ACGAGTTAAA AAAACAGCAC 
CTTTTAGAAA TGGAGGCTTG CAAGCAGAAG ATGACGGAAC AGGCGGCAGA ACTGGAAGTT 
AAACTGGCAG CTGAGAAGCA ACAGACGGAG TATTTATCAC ATGAACTTGA AGCCGCCAGA 
CTTGAACTTC AGTGCCTTGA CCTGAGTGCA CGCTCATTCC TGTCTTTTGA TACTGAAGAT 
TTAACAAAAA CACTGGAAGT TGCAAACCAG AGTATCTGCA CCGTTTTGCC CATAGGAAAG 
TTATCTCTTA GCAGCAGTGA ATTGCTCACC AACCATAATC TAAAAAACCT CATACAAAGT 
CTAGAATCAA CCAACTCTGA GACCAAAAAG GACAGAAGCA TTCTGGCAAC TAACTGCCCG 
GAAATACCAA ATGAGGGAGA TGAAAATGCC TCAAGTCTCG AAGAAAACTC TGCCGACTCG 
ACAAATAGTA ACATTTCAGA TTCTCCCGTC AGTAAACTGA GTTTGCAAGA AATTATGGAA 
ACTCTAAAGA TGCAGATTCA TCAAACCACT GCTGAAAATC TTAAACTGCT TCAGTCGCTC 
GAGGCTGGAG AGAAGAAAAA TCTAAATTTG TCGGCAGAAA TAGAATCTCT TAATAAACAG 
ATTGAGCAGC ATTCTGGGAA GGACAATATC GGCACCGAGC TTCAAAGCAG AGTAAAAGAG 
CTTGACGCTG AAAAGCTGCA GTTGTTTGAA AAATATGAAT CGATTTCTCA AGAGAAACTG 
CAATTAACCG GTCGAGTTGG GGACCTTGAG AAGGAACTTG CTAGCATTTC TAATGCAATG 
GAGGTGCTGA AAGAACAACT GTCTCAATTG TCCGGCATAA GGGAGAGTTT AGAAATAGCA 
AACGGAAATC TAAAGGAACA GTATCTGGAG ACAGAAAATG AAGTAAGAAG GGTTAAATCA 
GAAAGAGCAA ACATGGAGAA CCATGCCTTA TCTCTTGAAA TAGATCTAGA CACAATTCAG 
GCTAAATACC AGCAGTTGCA AGAGGAAAAC GAAGAGTACC GAAGATCTGC TGCAACTTTA 
CAAGACCGTC TCAGTGTTGT TGTGGCAGAG AAGAATCAAA TTGATCAGGA GCTGGAGAAT 
TTAACTGAAG AGAAAGATGA GCTTGAGCAA ATGTGTCAGA AACTGAAGGA AAGGGTGGAA 
GACCTTGAAT TAAATAAAGT GCACAACAGA AATCTTATTA AGATATTGGA AACTGAATTG 
CGGTCTTTAA AAGCCGAACT TCAGGGAGCA AAAGCATCAT TGGAGCAGTT GTCTGCTGAA 
AAAGATCTAA GGGATCTGCA GGAATCTGAA AAGAATGTGC ATGTGGAAGC AGAAGGGCTG 
AAGAACCAGC TGCAACAAAT ACAAGGGGAG TATCAGCTTT TGTTAAAAGA CTCTGAAGAT 
ATGCAGGCCC AGCTGAGTAA AGTGTGCAGC GAAAAGGATA AAATCTCCAA AGTTCTAGAG 
TGTTGTCAAT ATGAGAAACG GGAATTGGCC ACAAACTTGA GCTCTGCTCA GGAAGAAGTT 
GCTCAGATGC GGGCTGGGAT TGAGAAGCTC AAGGTTCGAA TGGAGTCAGA TGAAAAGAAG 
AAGAATCACT TGATCGGGAA ACTAAAAGAG ACTGAAAGGA ATTCAGATCA TCTGAAAGAT 
AAAATCGAGA ATCTTGAACG GGAATTGCTT ATGTCGGAGG AAAATCTCGA GTCCACAATC 
CTTCAGTCGG AATCTTCTAA GGAAGAAGTA GAGAAACTAA AGTCGATGAA GGAGGCGCTA 
GAAGCTAACG TGAACACCTT CAGGAGGAGG ATTGTAGATC TAGAAAGAGA ACTTGAGAAA 
AGCAAAGAGA GGATAGAAGA ACTGGAAACC AGAGTACTTA CCCTTTCAAA TGCCCTGGAA 
AAGAGCGAAA TGGAGAAGAG TTGTCTCAAT GAGGAGTCCG GACAAGAACT TTTGTTGCTG 
CGGGCCCAGC TAAATGAGTT GCAAGAGCAA AAAAGAGCTT CTGCAAAACA GTCAGATCTT 
GAAGCTCTTC TGGAACAGAA TAAGATGCAG CTGATGCAGC AACTGGAGGA AGCACACAAT 
ACAAGCAGGA CCCTGGAATT AACCATGGAG AAAGTCACGT TGGAATTAAG GGAATGCAAA 
TTGCAACTTG ATGAAAAGAC TGAGCTGGTT CTGAGTCTAG AAGGCAAATT GGAAGAAGCA 
GAGAATTTGG AAAAGAAACT GGCTGAGCTA GCTGAGCAGA AGGCCCTTTC TGATGAGAAG 
TGCAATGCAG CTGCTACCAA GAATGCTGAG CTCCTTATTT TAATTGACCA AAATAAAGCA 
GAGCTGTTGC ACCAGGTAGG AGAAGCGCAA GGAGAGACCA GGAACATGGA ACTGTCTCTT 
CAGAAAATTG CTACAGAACT GGGTGAAACA AAGAGGAGAC TGGAAGAAAA GGAACAGCAG 
ATCTTTGTTC TAGAATGCAA GTTGGAAGAG ACCATTGAGT CTGAAAAGAA ATGCCATTTG 
GAACTGTTGC AGTGTGAAGC AGAAAGAGAC CGTCTAAACG GAGAAATTGA GAACCAGCAA 
AGAGATCTGG AGCTGCTACA ATGCAAGGTT CAGATGGAGT CTGGGGCCAG TGAGTCTTCT 
CAGGCAGCAA TCCGTGATCT GAAATCCACT TGCAACGATC TAGAAGCCCA GCTCCAATCT 
TCTGATGCGG AGAAGCAAGT TCTTGTGCAG AAAGTAGATG AGCTGACAGA AAACTGTACC 
ATCTTACAAA GCAAAGTACA TGAGGCGGAA ATGAAAATAA AGGAAAAGAA GGTGCTGGAT 
GAGCAACTGC AGGCAGTTAA GCAGCAAATT GAAGACACTA ATACACGGCT CTGTGCCACA 
ACCACAGAAA AAACTGAACT AACGGAGACG ATCGCCAAAC TCCGCATTGA GCAGGAGGCG 
CAGGCAGATC GGAGCCGCAG TGACGCAAGG GAATTGCAGA ACAGACTTCT TCAGGCCGAG 
GCTAAGCAGC AGGCTGCCCT GGATGCGCTA CTGATACAGC ACAAAGAGGA AATCCAGGCT 
TTGCAAGGCA AACTCACCTT GGCCGAAGAG CATGCGATTG GGCACGGATA TGAGGTTGAA 
CGGCTAAAAG CAGCTAATGT GGAACTGAAT GGCTCTCTGG GCAGTGCTCA CCGGCAACTG 
GAGGAGCTGA ACCAACTGAA GGTGAGTTTT GCTGAGCTGA AGGAAGAACA CGCGCGCGTC 
TGCGCTGCCC TCAGGCACCA GGTGGAGTCC TGCGCAGAAC TGGAGCAGGA GAGAGATCGG 
CTGAAGGGAA AGATAAACGA GAGAGAAGAG GAATTAAAAC ATCTTCAGGC AAAACAGCAA 
AATGCAGAGG GCGGAGCTAG CTCTAACGAG GACTTATTGG CTGAGATCGA GGAACTGAAA 
CAGTGGTTGG AGGAGAAGAC TACAGAGGCA GACGAGAGTG TTGAGAAGTA CTGCACGATA 
ATGATTAAAT CCCACAAACT GGAAGAGGAC AATGACACCC TGAAGAAGCA ACTCGACTTC 
CTCAGCTCCA AACTGAAGCA GCTGCAAACA AGTCAAGAGG TCCCAAGCCC ACCCCAGAGA 
GGGGACCATC CAGGACCACA GAGCATGGAC CAAAAAGGAG GAGAAGACAA CCAGTCGGCA 
CAGAGAGTAT CTAAACAGTC TTCCAAGAGG CGGCGAGAGC AAGATGTTAC TTTAGATAAA 
GAAGAGCCAC GATCTCCAAT ACCCCAGGGT TTGGCCAAAA GAGTTAGAAA GACTGCGAGC 
CGTACTGTGC CCCATCAGCG TTCCACTGAG CATGAGGAAG AGTTCCAACC TGATGGGCTC 
CCAGATGTTG TACAAAAAGG GTTTGCCGAT ATCCCATCAG GAAAACAAAG TCCTTACATT 
CTAAGGAGAA CGACAGGGCC CGTAAGGAAA AGCCCCCGAC TCGCTGCACT TAAACCTTCT 
CCATCCACCC ACGAAGATCT GGAAAACCTT GCGCATTTCT CCAGTCCAGC AGCAGGGGGC 
AGCAAAGCAC AGCAGTCGAA ACTCTCAGAA GCCGTTAGGG CTGGATCAGA AGTTGGACCC 
ATGGAAGTGT CTTCCCCACT CTCGTCCTAC AACAGACTAA AGAACCCGGG GTCTGACAGT 
CCGATCAGCA GGAAAATGGC AATAGAAAGG ATCTCGGAGA AGCTTCTGCA TGAAGAGTCT 
GAGACTGAGG AAGCCTGTCA TGTACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002934738.2
CDS	299..8128
Translation	MSWVVEEWKEGLPTKTLQKIQELESQLDKLKKERQQRQFQLESLEAAFQKQKQKAESEKSEISALKRENQSLIESCDNQEKAKQKITHEYQLKESQVNFLEGQLSASKKQIEKLEQELKRYKSDLEKSQLSFNACDMSVCATPQKSFVAPYTPNKYTDFRYEELQEKYQKEVEERKRLETEMKTLRNVPQPPQAAAQSTMTHRDIARHQTSSSVFSWQEDRTPSHPSSTSHDKRLKRSFASSQLLWDQEETPSKRGVRSENLGKSASDSYTTQLNDQLKTQNQELKSRINELEMRLQVQEKDLKNQLNKLQESQALYEKTQRELAERDKTLVKCKEDLARVTAQCEQAANKCVMAEQKLKKVSDELSCQRQNAESARVAAEQKLKEREKENQQEILQQQNSLRNMEQQLSQIKAKMSQEAQQAKNDFNALQSELDRAIHTKKILENETEELRQRLSRAEQDLCQCRSNENDLRKSLEDTKSEKNVMKSQYDQKSKDALKLEEELQTANQTLRQNQLFVDEIKAKNLHLEAELKSAFEKLQDHDSATFLNLKATVHNLEMERNFAQELLKKRENDFEEIKNNLARMMDETNSLKSHLDHKEKECKELINANISLNSWKVEQEKVVGQISRDKDDLLKKIQELEHLLQTHGDQVHLLENDKKNLHTQIRTLQEIVDAKTVDLQNQQLVCGELRQNLDYENQKFNSENEKLQLQISELENAIKEQNCSASLDKSSYCENLLFQEKQLNSEMQKQMKELLLEKEDIQKCLNEAEVLHEQFVTGSRSHMESLQENISSKQNYIESIECAVREKEEQITTLTEKLGVLDANLQSVQEKNKELNDKLQELNLLSESWPLERERLNSQISLNREEIERLTEENKRTHQVNDPHNDQETNKESEHQLETTQPSAEDKVEPRGLPDGAGIENTNELEALQQELVHVKEKHCRLQREYDRLTNANEELTKLLDDLRNNEQSLDNITDLSAYLKEKENSLNNSNFVLETLPMDLDDEKQIHDGCTDSPTLKGTPNTVKSNNDLDLEQELSTLQKQDIFSFDIDQAERATNISDLLVNISDHQIGDRPSSSLDSSQCDDSINLNKDILGLRISGGWATTKPPQSISDNENSGLLTSPNEGTSKRLKETAENILKSPVPPSQRSSPSSMNSNNRGGREANRKSIPCKSAADSILGKSLEDQLWMMAVMASGDAGDFKEFLTTYQTEVDELKKQHLLEMEACKQKMTEQAAELEVKLAAEKQQTEYLSHELEAARLELQCLDLSARSFLSFDTEDLTKTLEVANQSICTVLPIGKLSLSSSELLTNHNLKNLIQSLESTNSETKKDRSILATNCPEIPNEGDENASSLEENSADSTNSNISDSPVSKLSLQEIMETLKMQIHQTTAENLKLLQSLEAGEKKNLNLSAEIESLNKQIEQHSGKDNIGTELQSRVKELDAEKLQLFEKYESISQEKLQLTGRVGDLEKELASISNAMEVLKEQLSQLSGIRESLEIANGNLKEQYLETENEVRRVKSERANMENHALSLEIDLDTIQAKYQQLQEENEEYRRSAATLQDRLSVVVAEKNQIDQELENLTEEKDELEQMCQKLKERVEDLELNKVHNRNLIKILETELRSLKAELQGAKASLEQLSAEKDLRDLQESEKNVHVEAEGLKNQLQQIQGEYQLLLKDSEDMQAQLSKVCSEKDKISKVLECCQYEKRELATNLSSAQEEVAQMRAGIEKLKVRMESDEKKKNHLIGKLKETERNSDHLKDKIENLERELLMSEENLESTILQSESSKEEVEKLKSMKEALEANVNTFRRRIVDLERELEKSKERIEELETRVLTLSNALEKSEMEKSCLNEESGQELLLLRAQLNELQEQKRASAKQSDLEALLEQNKMQLMQQLEEAHNTSRTLELTMEKVTLELRECKLQLDEKTELVLSLEGKLEEAENLEKKLAELAEQKALSDEKCNAAATKNAELLILIDQNKAELLHQVGEAQGETRNMELSLQKIATELGETKRRLEEKEQQIFVLECKLEETIESEKKCHLELLQCEAERDRLNGEIENQQRDLELLQCKVQMESGASESSQAAIRDLKSTCNDLEAQLQSSDAEKQVLVQKVDELTENCTILQSKVHEAEMKIKEKKVLDEQLQAVKQQIEDTNTRLCATTTEKTELTETIAKLRIEQEAQADRSRSDARELQNRLLQAEAKQQAALDALLIQHKEEIQALQGKLTLAEEHAIGHGYEVERLKAANVELNGSLGSAHRQLEELNQLKVSFAELKEEHARVCAALRHQVESCAELEQERDRLKGKINEREEELKHLQAKQQNAEGGASSNEDLLAEIEELKQWLEEKTTEADESVEKYCTIMIKSHKLEEDNDTLKKQLDFLSSKLKQLQTSQEVPSPPQRGDHPGPQSMDQKGGEDNQSAQRVSKQSSKRRREQDVTLDKEEPRSPIPQGLAKRVRKTASRTVPHQRSTEHEEEFQPDGLPDVVQKGFADIPSGKQSPYILRRTTGPVRKSPRLAALKPSPSTHEDLENLAHFSSPAAGGSKAQQSKLSEAVRAGSEVGPMEVSSPLSSYNRLKNPGSDSPISRKMAIERISEKLLHEESETEEACHVQ

Target ORF information:

RefSeq Version	XM_002934692.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis centromere protein F (cenpf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934692.4

ORF Insert Sequence:

ATGAGTTGGG TCGTAGAGGA GTGGAAGGAG GGGCTGCCCA CAAAGACCCT TCAGAAGATC 
CAGGAGCTGG AAAGTCAGTT GGACAAACTA AAGAAAGAGC GGCAGCAAAG GCAGTTTCAG 
CTGGAGTCGC TGGAAGCCGC TTTCCAAAAG CAGAAGCAAA AGGCAGAAAG TGAAAAAAGC 
GAAATCTCCG CCCTAAAACG AGAGAATCAG AGTTTGATAG AATCATGTGA CAATCAGGAG 
AAGGCAAAGC AGAAGATTAC CCATGAGTAC CAGCTAAAAG AGTCACAGGT CAACTTCCTG 
GAGGGCCAAC TCAGCGCCAG CAAGAAGCAA ATCGAAAAAC TGGAGCAAGA GCTTAAAAGG 
TACAAGAGTG ACTTGGAGAA AAGCCAACTG TCGTTCAATG CCTGTGACAT GTCTGTGTGC 
GCCACTCCCC AGAAGAGCTT TGTGGCGCCA TATACACCCA ATAAATACAC TGATTTCAGG 
TATGAAGAAT TGCAAGAGAA ATATCAGAAA GAGGTGGAAG AGAGGAAGAG GTTGGAAACA 
GAGATGAAAA CATTAAGGAA TGTGCCCCAG CCTCCACAGG CCGCTGCCCA GAGCACCATG 
ACGCACCGGG ATATCGCGCG GCACCAGACT TCCTCGTCTG TGTTCTCGTG GCAAGAAGAT 
CGAACCCCAA GTCACCCCTC ATCTACCAGT CATGATAAAA GACTTAAGAG AAGTTTTGCA 
TCATCCCAGC TCCTGTGGGA CCAGGAGGAA ACTCCAAGCA AAAGAGGTGT CCGATCAGAG 
AACTTGGGTA AAAGTGCCAG TGACTCCTAC ACCACTCAGC TTAATGATCA GCTAAAAACC 
CAGAATCAAG AGCTGAAATC CAGAATAAAT GAGCTGGAGA TGCGCTTGCA AGTTCAAGAG 
AAAGATCTGA AAAATCAGCT TAACAAACTC CAGGAAAGCC AAGCTCTGTA TGAAAAAACC 
CAAAGGGAAC TCGCAGAGAG AGACAAAACC CTGGTGAAAT GCAAGGAGGA CTTGGCACGT 
GTGACTGCGC AGTGTGAGCA GGCTGCTAAT AAGTGTGTGA TGGCGGAACA GAAGTTAAAG 
AAAGTTTCAG ATGAGCTGAG CTGCCAGAGG CAAAATGCTG AGAGTGCGAG GGTTGCTGCG 
GAGCAGAAAC TAAAGGAACG AGAGAAGGAA AACCAACAGG AGATTTTACA GCAGCAGAAT 
TCCCTAAGGA ATATGGAGCA GCAGCTCAGC CAGATAAAAG CCAAGATGAG CCAGGAGGCA 
CAGCAAGCCA AAAATGACTT CAACGCCCTC CAATCAGAGC TTGACCGGGC CATTCACACG 
AAAAAGATTT TGGAAAACGA AACAGAAGAA CTAAGGCAGA GGCTCAGCCG AGCGGAACAG 
GACTTGTGTC AGTGCCGAAG TAACGAAAAT GATTTAAGGA AGAGCCTTGA GGACACCAAG 
TCGGAAAAAA ATGTAATGAA AAGCCAGTAT GATCAGAAAT CTAAAGACGC TCTGAAACTG 
GAAGAAGAAT TACAAACGGC GAACCAAACC CTGAGGCAGA ACCAGCTCTT TGTGGATGAA 
ATAAAAGCTA AGAATCTTCA TTTAGAAGCA GAACTGAAGT CAGCTTTTGA GAAACTGCAA 
GACCACGACT CTGCCACCTT CCTGAATTTG AAAGCCACGG TGCATAATTT GGAAATGGAG 
AGGAATTTTG CCCAAGAATT ATTAAAGAAA AGAGAGAATG ATTTTGAAGA AATTAAAAAT 
AATTTAGCGA GAATGATGGA TGAAACAAAC TCATTAAAAA GTCACCTTGA TCACAAAGAA 
AAAGAATGTA AGGAACTGAT AAATGCTAAT ATTTCACTTA ATAGCTGGAA AGTGGAACAG 
GAGAAGGTGG TGGGTCAAAT TAGCAGAGAT AAAGATGATC TGCTGAAGAA GATTCAGGAG 
TTGGAACATT TGCTGCAGAC TCACGGTGAC CAAGTACACT TGTTAGAAAA TGACAAGAAA 
AACTTGCACA CTCAAATTAG AACCTTGCAA GAGATCGTCG ATGCCAAGAC CGTGGACCTG 
CAAAATCAGC AACTGGTATG CGGTGAACTT CGACAAAATC TGGACTACGA AAACCAGAAA 
TTTAATAGCG AAAATGAAAA GTTGCAGCTA CAAATTTCCG AGCTTGAGAA TGCAATCAAA 
GAGCAAAATT GTTCCGCTAG TTTGGACAAA TCGTCTTATT GCGAAAACTT GCTATTCCAG 
GAGAAGCAAT TGAATTCCGA AATGCAAAAA CAGATGAAAG AACTGTTATT AGAGAAGGAA 
GATATCCAGA AATGTCTAAA TGAAGCAGAG GTATTGCACG AACAGTTTGT CACAGGCTCC 
CGAAGCCACA TGGAGAGCTT GCAGGAAAAC ATTTCTTCCA AGCAGAACTA CATTGAGTCA 
ATAGAATGTG CAGTTAGAGA GAAAGAAGAG CAAATTACCA CTTTAACCGA AAAATTAGGA 
GTTCTAGATG CAAACTTGCA ATCTGTCCAG GAGAAAAACA AAGAGCTTAA TGATAAACTG 
CAGGAGCTCA ACCTGCTTTC TGAATCGTGG CCTTTAGAGA GAGAAAGACT AAATTCCCAG 
ATTAGTTTGA ATCGGGAGGA AATTGAACGG CTGACTGAAG AAAATAAAAG AACCCATCAA 
GTCAATGATC CTCACAATGA TCAGGAAACA AACAAAGAAT CGGAGCACCA GTTAGAGACT 
ACCCAGCCAT CGGCAGAAGA TAAAGTTGAG CCTAGAGGGT TGCCTGATGG GGCGGGTATT 
GAGAATACCA ATGAGCTGGA AGCACTTCAG CAAGAACTCG TTCATGTAAA AGAAAAACAC 
TGTAGACTGC AAAGAGAATA TGACAGACTC ACTAATGCCA ACGAGGAACT TACCAAGCTG 
CTTGATGACC TCCGAAACAA CGAACAGTCT CTCGATAACA TCACCGATTT AAGTGCTTAT 
TTGAAGGAAA AGGAAAATTC TCTAAATAAC AGTAATTTTG TGCTTGAGAC TCTTCCAATG 
GATCTTGATG ATGAGAAACA AATTCACGAT GGATGCACTG ATTCTCCTAC ACTAAAGGGT 
ACACCAAACA CCGTGAAGTC CAACAATGAT CTAGATTTAG AACAAGAGCT ATCTACACTT 
CAAAAACAGG ACATATTTTC ATTTGATATC GACCAGGCAG AACGTGCCAC CAATATTTCA 
GACCTTCTGG TGAACATCAG TGACCATCAA ATAGGTGATC GTCCATCCAG TAGTCTTGAT 
TCCAGCCAGT GCGATGACAG TATTAACCTT AATAAAGACA TTTTAGGCCT TCGAATATCT 
GGTGGTTGGG CAACTACAAA ACCACCTCAG TCTATTAGTG ACAACGAAAA TAGTGGTCTC 
TTGACTTCAC CGAACGAGGG CACATCAAAA CGTTTGAAAG AGACTGCAGA AAACATATTG 
AAATCACCAG TTCCTCCAAG TCAGCGTAGT AGTCCATCCT CAATGAATTC CAATAACAGA 
GGAGGCCGTG AAGCCAATAG AAAGTCTATC CCTTGCAAAT CTGCTGCTGA CTCCATTCTT 
GGGAAATCAC TCGAAGATCA GTTATGGATG ATGGCTGTTA TGGCCAGCGG AGATGCCGGA 
GACTTTAAAG AATTTCTTAC AACTTACCAA ACAGAAGTGG ACGAGTTAAA AAAACAGCAC 
CTTTTAGAAA TGGAGGCTTG CAAGCAGAAG ATGACGGAAC AGGCGGCAGA ACTGGAAGTT 
AAACTGGCAG CTGAGAAGCA ACAGACGGAG TATTTATCAC ATGAACTTGA AGCCGCCAGA 
CTTGAACTTC AGTGCCTTGA CCTGAGTGCA CGCTCATTCC TGTCTTTTGA TACTGAAGAT 
TTAACAAAAA CACTGGAAGT TGCAAACCAG AGTATCTGCA CCGTTTTGCC CATAGGAAAG 
TTATCTCTTA GCAGCAGTGA ATTGCTCACC AACCATAATC TAAAAAACCT CATACAAAGT 
CTAGAATCAA CCAACTCTGA GACCAAAAAG GACAGAAGCA TTCTGGCAAC TAACTGCCCG 
GAAATACCAA ATGAGGGAGA TGAAAATGCC TCAAGTCTCG AAGAAAACTC TGCCGACTCG 
ACAAATAGTA ACATTTCAGA TTCTCCCGTC AGTAAACTGA GTTTGCAAGA AATTATGGAA 
ACTCTAAAGA TGCAGATTCA TCAAACCACT GCTGAAAATC TTAAACTGCT TCAGTCGCTC 
GAGGCTGGAG AGAAGAAAAA TCTAAATTTG TCGGCAGAAA TAGAATCTCT TAATAAACAG 
ATTGAGCAGC ATTCTGGGAA GGACAATATC GGCACCGAGC TTCAAAGCAG AGTAAAAGAG 
CTTGACGCTG AAAAGCTGCA GTTGTTTGAA AAATATGAAT CGATTTCTCA AGAGAAACTG 
CAATTAACCG GTCGAGTTGG GGACCTTGAG AAGGAACTTG CTAGCATTTC TAATGCAATG 
GAGGTGCTGA AAGAACAACT GTCTCAATTG TCCGGCATAA GGGAGAGTTT AGAAATAGCA 
AACGGAAATC TAAAGGAACA GTATCTGGAG ACAGAAAATG AAGTAAGAAG GGTTAAATCA 
GAAAGAGCAA ACATGGAGAA CCATGCCTTA TCTCTTGAAA TAGATCTAGA CACAATTCAG 
GCTAAATACC AGCAGTTGCA AGAGGAAAAC GAAGAGTACC GAAGATCTGC TGCAACTTTA 
CAAGACCGTC TCAGTGTTGT TGTGGCAGAG AAGAATCAAA TTGATCAGGA GCTGGAGAAT 
TTAACTGAAG AGAAAGATGA GCTTGAGCAA ATGTGTCAGA AACTGAAGGA AAGGGTGGAA 
GACCTTGAAT TAAATAAAGT GCACAACAGA AATCTTATTA AGATATTGGA AACTGAATTG 
CGGTCTTTAA AAGCCGAACT TCAGGGAGCA AAAGCATCAT TGGAGCAGTT GTCTGCTGAA 
AAAGATCTAA GGGATCTGCA GGAATCTGAA AAGAATGTGC ATGTGGAAGC AGAAGGGCTG 
AAGAACCAGC TGCAACAAAT ACAAGGGGAG TATCAGCTTT TGTTAAAAGA CTCTGAAGAT 
ATGCAGGCCC AGCTGAGTAA AGTGTGCAGC GAAAAGGATA AAATCTCCAA AGTTCTAGAG 
TGTTGTCAAT ATGAGAAACG GGAATTGGCC ACAAACTTGA GCTCTGCTCA GGAAGAAGTT 
GCTCAGATGC GGGCTGGGAT TGAGAAGCTC AAGGTTCGAA TGGAGTCAGA TGAAAAGAAG 
AAGAATCACT TGATCGGGAA ACTAAAAGAG ACTGAAAGGA ATTCAGATCA TCTGAAAGAT 
AAAATCGAGA ATCTTGAACG GGAATTGCTT ATGTCGGAGG AAAATCTCGA GTCCACAATC 
CTTCAGTCGG AATCTTCTAA GGAAGAAGTA GAGAAACTAA AGTCGATGAA GGAGGCGCTA 
GAAGCTAACG TGAACACCTT CAGGAGGAGG ATTGTAGATC TAGAAAGAGA ACTTGAGAAA 
AGCAAAGAGA GGATAGAAGA ACTGGAAACC AGAGTACTTA CCCTTTCAAA TGCCCTGGAA 
AAGAGCGAAA TGGAGAAGAG TTGTCTCAAT GAGGAGTCCG GACAAGAACT TTTGTTGCTG 
CGGGCCCAGC TAAATGAGTT GCAAGAGCAA AAAAGAGCTT CTGCAAAACA GTCAGATCTT 
GAAGCTCTTC TGGAACAGAA TAAGATGCAG CTGATGCAGC AACTGGAGGA AGCACACAAT 
ACAAGCAGGA CCCTGGAATT AACCATGGAG AAAGTCACGT TGGAATTAAG GGAATGCAAA 
TTGCAACTTG ATGAAAAGAC TGAGCTGGTT CTGAGTCTAG AAGGCAAATT GGAAGAAGCA 
GAGAATTTGG AAAAGAAACT GGCTGAGCTA GCTGAGCAGA AGGCCCTTTC TGATGAGAAG 
TGCAATGCAG CTGCTACCAA GAATGCTGAG CTCCTTATTT TAATTGACCA AAATAAAGCA 
GAGCTGTTGC ACCAGGTAGG AGAAGCGCAA GGAGAGACCA GGAACATGGA ACTGTCTCTT 
CAGAAAATTG CTACAGAACT GGGTGAAACA AAGAGGAGAC TGGAAGAAAA GGAACAGCAG 
ATCTTTGTTC TAGAATGCAA GTTGGAAGAG ACCATTGAGT CTGAAAAGAA ATGCCATTTG 
GAACTGTTGC AGTGTGAAGC AGAAAGAGAC CGTCTAAACG GAGAAATTGA GAACCAGCAA 
AGAGATCTGG AGCTGCTACA ATGCAAGGTT CAGATGGAGT CTGGGGCCAG TGAGTCTTCT 
CAGGCAGCAA TCCGTGATCT GAAATCCACT TGCAACGATC TAGAAGCCCA GCTCCAATCT 
TCTGATGCGG AGAAGCAAGT TCTTGTGCAG AAAGTAGATG AGCTGACAGA AAACTGTACC 
ATCTTACAAA GCAAAGTACA TGAGGCGGAA ATGAAAATAA AGGAAAAGAA GGTGCTGGAT 
GAGCAACTGC AGGCAGTTAA GCAGCAAATT GAAGACACTA ATACACGGCT CTGTGCCACA 
ACCACAGAAA AAACTGAACT AACGGAGACG ATCGCCAAAC TCCGCATTGA GCAGGAGGCG 
CAGGCAGATC GGAGCCGCAG TGACGCAAGG GAATTGCAGA ACAGACTTCT TCAGGCCGAG 
GCTAAGCAGC AGGCTGCCCT GGATGCGCTA CTGATACAGC ACAAAGAGGA AATCCAGGCT 
TTGCAAGGCA AACTCACCTT GGCCGAAGAG CATGCGATTG GGCACGGATA TGAGGTTGAA 
CGGCTAAAAG CAGCTAATGT GGAACTGAAT GGCTCTCTGG GCAGTGCTCA CCGGCAACTG 
GAGGAGCTGA ACCAACTGAA GGTGAGTTTT GCTGAGCTGA AGGAAGAACA CGCGCGCGTC 
TGCGCTGCCC TCAGGCACCA GGTGGAGTCC TGCGCAGAAC TGGAGCAGGA GAGAGATCGG 
CTGAAGGGAA AGATAAACGA GAGAGAAGAG GAATTAAAAC ATCTTCAGGC AAAACAGCAA 
AATGCAGAGG GCGGAGCTAG CTCTAACGAG GACTTATTGG CTGAGATCGA GGAACTGAAA 
CAGTGGTTGG AGGAGAAGAC TACAGAGGCA GACGAGAGTG TTGAGAAGTA CTGCACGATA 
ATGATTAAAT CCCACAAACT GGAAGAGGAC AATGACACCC TGAAGAAGCA ACTCGACTTC 
CTCAGCTCCA AACTGAAGCA GCTGCAAACA AGTCAAGAGG TCCCAAGCCC ACCCCAGAGA 
GGGGACCATC CAGGACCACA GAGCATGGAC CAAAAAGGAG GAGAAGACAA CCAGTCGGCA 
CAGAGAGTAT CTAAACAGTC TTCCAAGAGG CGGCGAGAGC AAGATGTTAC TTTAGATAAA 
GAAGAGCCAC GATCTCCAAT ACCCCAGGGT TTGGCCAAAA GAGTTAGAAA GACTGCGAGC 
CGTACTGTGC CCCATCAGCG TTCCACTGAG CATGAGGAAG AGTTCCAACC TGATGGGCTC 
CCAGATGTTG TACAAAAAGG GTTTGCCGAT ATCCCATCAG GAAAACAAAG TCCTTACATT 
CTAAGGAGAA CGACAGGGCC CGTAAGGAAA AGCCCCCGAC TCGCTGCACT TAAACCTTCT 
CCATCCACCC ACGAAGATCT GGAAAACCTT GCGCATTTCT CCAGTCCAGC AGCAGGGGGC 
AGCAAAGCAC AGCAGTCGAA ACTCTCAGAA GCCGTTAGGG CTGGATCAGA AGTTGGACCC 
ATGGAAGTGT CTTCCCCACT CTCGTCCTAC AACAGACTAA AGAACCCGGG GTCTGACAGT 
CCGATCAGCA GGAAAATGGC AATAGAAAGG ATCTCGGAGA AGCTTCTGCA TGAAGAGTCT 
GAGACTGAGG AAGCCTGTCA TGTACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cenpf ( XM_002934692.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934738.2 Xenopus tropicalis centromere protein F (cenpf), mRNA.

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CloneID	OXa10912
Clone ID Related Accession (Same CDS sequence)	XM_002934692.4 , XM_002934692.5
Accession Version	XM_002934692.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	centromere protein F
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002934692.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTGGG TCGTAGAGGA GTGGAAGGAG GGGCTGCCCA CAAAGACCCT TCAGAAGATC 
CAGGAGCTGG AAAGTCAGTT GGACAAACTA AAGAAAGAGC GGCAGCAAAG GCAGTTTCAG 
CTGGAGTCGC TGGAAGCCGC TTTCCAAAAG CAGAAGCAAA AGGCAGAAAG TGAAAAAAGC 
GAAATCTCCG CCCTAAAACG AGAGAATCAG AGTTTGATAG AATCATGTGA CAATCAGGAG 
AAGGCAAAGC AGAAGATTAC CCATGAGTAC CAGCTAAAAG AGTCACAGGT CAACTTCCTG 
GAGGGCCAAC TCAGCGCCAG CAAGAAGCAA ATCGAAAAAC TGGAGCAAGA GCTTAAAAGG 
TACAAGAGTG ACTTGGAGAA AAGCCAACTG TCGTTCAATG CCTGTGACAT GTCTGTGTGC 
GCCACTCCCC AGAAGAGCTT TGTGGCGCCA TATACACCCA ATAAATACAC TGATTTCAGG 
TATGAAGAAT TGCAAGAGAA ATATCAGAAA GAGGTGGAAG AGAGGAAGAG GTTGGAAACA 
GAGATGAAAA CATTAAGGAA TGTGCCCCAG CCTCCACAGG CCGCTGCCCA GAGCACCATG 
ACGCACCGGG ATATCGCGCG GCACCAGACT TCCTCGTCTG TGTTCTCGTG GCAAGAAGAT 
CGAACCCCAA GTCACCCCTC ATCTACCAGT CATGATAAAA GACTTAAGAG AAGTTTTGCA 
TCATCCCAGC TCCTGTGGGA CCAGGAGGAA ACTCCAAGCA AAAGAGGTGT CCGATCAGAG 
AACTTGGGTA AAAGTGCCAG TGACTCCTAC ACCACTCAGC TTAATGATCA GCTAAAAACC 
CAGAATCAAG AGCTGAAATC CAGAATAAAT GAGCTGGAGA TGCGCTTGCA AGTTCAAGAG 
AAAGATCTGA AAAATCAGCT TAACAAACTC CAGGAAAGCC AAGCTCTGTA TGAAAAAACC 
CAAAGGGAAC TCGCAGAGAG AGACAAAACC CTGGTGAAAT GCAAGGAGGA CTTGGCACGT 
GTGACTGCGC AGTGTGAGCA GGCTGCTAAT AAGTGTGTGA TGGCGGAACA GAAGTTAAAG 
AAAGTTTCAG ATGAGCTGAG CTGCCAGAGG CAAAATGCTG AGAGTGCGAG GGTTGCTGCG 
GAGCAGAAAC TAAAGGAACG AGAGAAGGAA AACCAACAGG AGATTTTACA GCAGCAGAAT 
TCCCTAAGGA ATATGGAGCA GCAGCTCAGC CAGATAAAAG CCAAGATGAG CCAGGAGGCA 
CAGCAAGCCA AAAATGACTT CAACGCCCTC CAATCAGAGC TTGACCGGGC CATTCACACG 
AAAAAGATTT TGGAAAACGA AACAGAAGAA CTAAGGCAGA GGCTCAGCCG AGCGGAACAG 
GACTTGTGTC AGTGCCGAAG TAACGAAAAT GATTTAAGGA AGAGCCTTGA GGACACCAAG 
TCGGAAAAAA ATGTAATGAA AAGCCAGTAT GATCAGAAAT CTAAAGACGC TCTGAAACTG 
GAAGAAGAAT TACAAACGGC GAACCAAACC CTGAGGCAGA ACCAGCTCTT TGTGGATGAA 
ATAAAAGCTA AGAATCTTCA TTTAGAAGCA GAACTGAAGT CAGCTTTTGA GAAACTGCAA 
GACCACGACT CTGCCACCTT CCTGAATTTG AAAGCCACGG TGCATAATTT GGAAATGGAG 
AGGAATTTTG CCCAAGAATT ATTAAAGAAA AGAGAGAATG ATTTTGAAGA AATTAAAAAT 
AATTTAGCGA GAATGATGGA TGAAACAAAC TCATTAAAAA GTCACCTTGA TCACAAAGAA 
AAAGAATGTA AGGAACTGAT AAATGCTAAT ATTTCACTTA ATAGCTGGAA AGTGGAACAG 
GAGAAGGTGG TGGGTCAAAT TAGCAGAGAT AAAGATGATC TGCTGAAGAA GATTCAGGAG 
TTGGAACATT TGCTGCAGAC TCACGGTGAC CAAGTACACT TGTTAGAAAA TGACAAGAAA 
AACTTGCACA CTCAAATTAG AACCTTGCAA GAGATCGTCG ATGCCAAGAC CGTGGACCTG 
CAAAATCAGC AACTGGTATG CGGTGAACTT CGACAAAATC TGGACTACGA AAACCAGAAA 
TTTAATAGCG AAAATGAAAA GTTGCAGCTA CAAATTTCCG AGCTTGAGAA TGCAATCAAA 
GAGCAAAATT GTTCCGCTAG TTTGGACAAA TCGTCTTATT GCGAAAACTT GCTATTCCAG 
GAGAAGCAAT TGAATTCCGA AATGCAAAAA CAGATGAAAG AACTGTTATT AGAGAAGGAA 
GATATCCAGA AATGTCTAAA TGAAGCAGAG GTATTGCACG AACAGTTTGT CACAGGCTCC 
CGAAGCCACA TGGAGAGCTT GCAGGAAAAC ATTTCTTCCA AGCAGAACTA CATTGAGTCA 
ATAGAATGTG CAGTTAGAGA GAAAGAAGAG CAAATTACCA CTTTAACCGA AAAATTAGGA 
GTTCTAGATG CAAACTTGCA ATCTGTCCAG GAGAAAAACA AAGAGCTTAA TGATAAACTG 
CAGGAGCTCA ACCTGCTTTC TGAATCGTGG CCTTTAGAGA GAGAAAGACT AAATTCCCAG 
ATTAGTTTGA ATCGGGAGGA AATTGAACGG CTGACTGAAG AAAATAAAAG AACCCATCAA 
GTCAATGATC CTCACAATGA TCAGGAAACA AACAAAGAAT CGGAGCACCA GTTAGAGACT 
ACCCAGCCAT CGGCAGAAGA TAAAGTTGAG CCTAGAGGGT TGCCTGATGG GGCGGGTATT 
GAGAATACCA ATGAGCTGGA AGCACTTCAG CAAGAACTCG TTCATGTAAA AGAAAAACAC 
TGTAGACTGC AAAGAGAATA TGACAGACTC ACTAATGCCA ACGAGGAACT TACCAAGCTG 
CTTGATGACC TCCGAAACAA CGAACAGTCT CTCGATAACA TCACCGATTT AAGTGCTTAT 
TTGAAGGAAA AGGAAAATTC TCTAAATAAC AGTAATTTTG TGCTTGAGAC TCTTCCAATG 
GATCTTGATG ATGAGAAACA AATTCACGAT GGATGCACTG ATTCTCCTAC ACTAAAGGGT 
ACACCAAACA CCGTGAAGTC CAACAATGAT CTAGATTTAG AACAAGAGCT ATCTACACTT 
CAAAAACAGG ACATATTTTC ATTTGATATC GACCAGGCAG AACGTGCCAC CAATATTTCA 
GACCTTCTGG TGAACATCAG TGACCATCAA ATAGGTGATC GTCCATCCAG TAGTCTTGAT 
TCCAGCCAGT GCGATGACAG TATTAACCTT AATAAAGACA TTTTAGGCCT TCGAATATCT 
GGTGGTTGGG CAACTACAAA ACCACCTCAG TCTATTAGTG ACAACGAAAA TAGTGGTCTC 
TTGACTTCAC CGAACGAGGG CACATCAAAA CGTTTGAAAG AGACTGCAGA AAACATATTG 
AAATCACCAG TTCCTCCAAG TCAGCGTAGT AGTCCATCCT CAATGAATTC CAATAACAGA 
GGAGGCCGTG AAGCCAATAG AAAGTCTATC CCTTGCAAAT CTGCTGCTGA CTCCATTCTT 
GGGAAATCAC TCGAAGATCA GTTATGGATG ATGGCTGTTA TGGCCAGCGG AGATGCCGGA 
GACTTTAAAG AATTTCTTAC AACTTACCAA ACAGAAGTGG ACGAGTTAAA AAAACAGCAC 
CTTTTAGAAA TGGAGGCTTG CAAGCAGAAG ATGACGGAAC AGGCGGCAGA ACTGGAAGTT 
AAACTGGCAG CTGAGAAGCA ACAGACGGAG TATTTATCAC ATGAACTTGA AGCCGCCAGA 
CTTGAACTTC AGTGCCTTGA CCTGAGTGCA CGCTCATTCC TGTCTTTTGA TACTGAAGAT 
TTAACAAAAA CACTGGAAGT TGCAAACCAG AGTATCTGCA CCGTTTTGCC CATAGGAAAG 
TTATCTCTTA GCAGCAGTGA ATTGCTCACC AACCATAATC TAAAAAACCT CATACAAAGT 
CTAGAATCAA CCAACTCTGA GACCAAAAAG GACAGAAGCA TTCTGGCAAC TAACTGCCCG 
GAAATACCAA ATGAGGGAGA TGAAAATGCC TCAAGTCTCG AAGAAAACTC TGCCGACTCG 
ACAAATAGTA ACATTTCAGA TTCTCCCGTC AGTAAACTGA GTTTGCAAGA AATTATGGAA 
ACTCTAAAGA TGCAGATTCA TCAAACCACT GCTGAAAATC TTAAACTGCT TCAGTCGCTC 
GAGGCTGGAG AGAAGAAAAA TCTAAATTTG TCGGCAGAAA TAGAATCTCT TAATAAACAG 
ATTGAGCAGC ATTCTGGGAA GGACAATATC GGCACCGAGC TTCAAAGCAG AGTAAAAGAG 
CTTGACGCTG AAAAGCTGCA GTTGTTTGAA AAATATGAAT CGATTTCTCA AGAGAAACTG 
CAATTAACCG GTCGAGTTGG GGACCTTGAG AAGGAACTTG CTAGCATTTC TAATGCAATG 
GAGGTGCTGA AAGAACAACT GTCTCAATTG TCCGGCATAA GGGAGAGTTT AGAAATAGCA 
AACGGAAATC TAAAGGAACA GTATCTGGAG ACAGAAAATG AAGTAAGAAG GGTTAAATCA 
GAAAGAGCAA ACATGGAGAA CCATGCCTTA TCTCTTGAAA TAGATCTAGA CACAATTCAG 
GCTAAATACC AGCAGTTGCA AGAGGAAAAC GAAGAGTACC GAAGATCTGC TGCAACTTTA 
CAAGACCGTC TCAGTGTTGT TGTGGCAGAG AAGAATCAAA TTGATCAGGA GCTGGAGAAT 
TTAACTGAAG AGAAAGATGA GCTTGAGCAA ATGTGTCAGA AACTGAAGGA AAGGGTGGAA 
GACCTTGAAT TAAATAAAGT GCACAACAGA AATCTTATTA AGATATTGGA AACTGAATTG 
CGGTCTTTAA AAGCCGAACT TCAGGGAGCA AAAGCATCAT TGGAGCAGTT GTCTGCTGAA 
AAAGATCTAA GGGATCTGCA GGAATCTGAA AAGAATGTGC ATGTGGAAGC AGAAGGGCTG 
AAGAACCAGC TGCAACAAAT ACAAGGGGAG TATCAGCTTT TGTTAAAAGA CTCTGAAGAT 
ATGCAGGCCC AGCTGAGTAA AGTGTGCAGC GAAAAGGATA AAATCTCCAA AGTTCTAGAG 
TGTTGTCAAT ATGAGAAACG GGAATTGGCC ACAAACTTGA GCTCTGCTCA GGAAGAAGTT 
GCTCAGATGC GGGCTGGGAT TGAGAAGCTC AAGGTTCGAA TGGAGTCAGA TGAAAAGAAG 
AAGAATCACT TGATCGGGAA ACTAAAAGAG ACTGAAAGGA ATTCAGATCA TCTGAAAGAT 
AAAATCGAGA ATCTTGAACG GGAATTGCTT ATGTCGGAGG AAAATCTCGA GTCCACAATC 
CTTCAGTCGG AATCTTCTAA GGAAGAAGTA GAGAAACTAA AGTCGATGAA GGAGGCGCTA 
GAAGCTAACG TGAACACCTT CAGGAGGAGG ATTGTAGATC TAGAAAGAGA ACTTGAGAAA 
AGCAAAGAGA GGATAGAAGA ACTGGAAACC AGAGTACTTA CCCTTTCAAA TGCCCTGGAA 
AAGAGCGAAA TGGAGAAGAG TTGTCTCAAT GAGGAGTCCG GACAAGAACT TTTGTTGCTG 
CGGGCCCAGC TAAATGAGTT GCAAGAGCAA AAAAGAGCTT CTGCAAAACA GTCAGATCTT 
GAAGCTCTTC TGGAACAGAA TAAGATGCAG CTGATGCAGC AACTGGAGGA AGCACACAAT 
ACAAGCAGGA CCCTGGAATT AACCATGGAG AAAGTCACGT TGGAATTAAG GGAATGCAAA 
TTGCAACTTG ATGAAAAGAC TGAGCTGGTT CTGAGTCTAG AAGGCAAATT GGAAGAAGCA 
GAGAATTTGG AAAAGAAACT GGCTGAGCTA GCTGAGCAGA AGGCCCTTTC TGATGAGAAG 
TGCAATGCAG CTGCTACCAA GAATGCTGAG CTCCTTATTT TAATTGACCA AAATAAAGCA 
GAGCTGTTGC ACCAGGTAGG AGAAGCGCAA GGAGAGACCA GGAACATGGA ACTGTCTCTT 
CAGAAAATTG CTACAGAACT GGGTGAAACA AAGAGGAGAC TGGAAGAAAA GGAACAGCAG 
ATCTTTGTTC TAGAATGCAA GTTGGAAGAG ACCATTGAGT CTGAAAAGAA ATGCCATTTG 
GAACTGTTGC AGTGTGAAGC AGAAAGAGAC CGTCTAAACG GAGAAATTGA GAACCAGCAA 
AGAGATCTGG AGCTGCTACA ATGCAAGGTT CAGATGGAGT CTGGGGCCAG TGAGTCTTCT 
CAGGCAGCAA TCCGTGATCT GAAATCCACT TGCAACGATC TAGAAGCCCA GCTCCAATCT 
TCTGATGCGG AGAAGCAAGT TCTTGTGCAG AAAGTAGATG AGCTGACAGA AAACTGTACC 
ATCTTACAAA GCAAAGTACA TGAGGCGGAA ATGAAAATAA AGGAAAAGAA GGTGCTGGAT 
GAGCAACTGC AGGCAGTTAA GCAGCAAATT GAAGACACTA ATACACGGCT CTGTGCCACA 
ACCACAGAAA AAACTGAACT AACGGAGACG ATCGCCAAAC TCCGCATTGA GCAGGAGGCG 
CAGGCAGATC GGAGCCGCAG TGACGCAAGG GAATTGCAGA ACAGACTTCT TCAGGCCGAG 
GCTAAGCAGC AGGCTGCCCT GGATGCGCTA CTGATACAGC ACAAAGAGGA AATCCAGGCT 
TTGCAAGGCA AACTCACCTT GGCCGAAGAG CATGCGATTG GGCACGGATA TGAGGTTGAA 
CGGCTAAAAG CAGCTAATGT GGAACTGAAT GGCTCTCTGG GCAGTGCTCA CCGGCAACTG 
GAGGAGCTGA ACCAACTGAA GGTGAGTTTT GCTGAGCTGA AGGAAGAACA CGCGCGCGTC 
TGCGCTGCCC TCAGGCACCA GGTGGAGTCC TGCGCAGAAC TGGAGCAGGA GAGAGATCGG 
CTGAAGGGAA AGATAAACGA GAGAGAAGAG GAATTAAAAC ATCTTCAGGC AAAACAGCAA 
AATGCAGAGG GCGGAGCTAG CTCTAACGAG GACTTATTGG CTGAGATCGA GGAACTGAAA 
CAGTGGTTGG AGGAGAAGAC TACAGAGGCA GACGAGAGTG TTGAGAAGTA CTGCACGATA 
ATGATTAAAT CCCACAAACT GGAAGAGGAC AATGACACCC TGAAGAAGCA ACTCGACTTC 
CTCAGCTCCA AACTGAAGCA GCTGCAAACA AGTCAAGAGG TCCCAAGCCC ACCCCAGAGA 
GGGGACCATC CAGGACCACA GAGCATGGAC CAAAAAGGAG GAGAAGACAA CCAGTCGGCA 
CAGAGAGTAT CTAAACAGTC TTCCAAGAGG CGGCGAGAGC AAGATGTTAC TTTAGATAAA 
GAAGAGCCAC GATCTCCAAT ACCCCAGGGT TTGGCCAAAA GAGTTAGAAA GACTGCGAGC 
CGTACTGTGC CCCATCAGCG TTCCACTGAG CATGAGGAAG AGTTCCAACC TGATGGGCTC 
CCAGATGTTG TACAAAAAGG GTTTGCCGAT ATCCCATCAG GAAAACAAAG TCCTTACATT 
CTAAGGAGAA CGACAGGGCC CGTAAGGAAA AGCCCCCGAC TCGCTGCACT TAAACCTTCT 
CCATCCACCC ACGAAGATCT GGAAAACCTT GCGCATTTCT CCAGTCCAGC AGCAGGGGGC 
AGCAAAGCAC AGCAGTCGAA ACTCTCAGAA GCCGTTAGGG CTGGATCAGA AGTTGGACCC 
ATGGAAGTGT CTTCCCCACT CTCGTCCTAC AACAGACTAA AGAACCCGGG GTCTGACAGT 
CCGATCAGCA GGAAAATGGC AATAGAAAGG ATCTCGGAGA AGCTTCTGCA TGAAGAGTCT 
GAGACTGAGG AAGCCTGTCA TGTACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002934738.2
CDS	285..8114
Translation	MSWVVEEWKEGLPTKTLQKIQELESQLDKLKKERQQRQFQLESLEAAFQKQKQKAESEKSEISALKRENQSLIESCDNQEKAKQKITHEYQLKESQVNFLEGQLSASKKQIEKLEQELKRYKSDLEKSQLSFNACDMSVCATPQKSFVAPYTPNKYTDFRYEELQEKYQKEVEERKRLETEMKTLRNVPQPPQAAAQSTMTHRDIARHQTSSSVFSWQEDRTPSHPSSTSHDKRLKRSFASSQLLWDQEETPSKRGVRSENLGKSASDSYTTQLNDQLKTQNQELKSRINELEMRLQVQEKDLKNQLNKLQESQALYEKTQRELAERDKTLVKCKEDLARVTAQCEQAANKCVMAEQKLKKVSDELSCQRQNAESARVAAEQKLKEREKENQQEILQQQNSLRNMEQQLSQIKAKMSQEAQQAKNDFNALQSELDRAIHTKKILENETEELRQRLSRAEQDLCQCRSNENDLRKSLEDTKSEKNVMKSQYDQKSKDALKLEEELQTANQTLRQNQLFVDEIKAKNLHLEAELKSAFEKLQDHDSATFLNLKATVHNLEMERNFAQELLKKRENDFEEIKNNLARMMDETNSLKSHLDHKEKECKELINANISLNSWKVEQEKVVGQISRDKDDLLKKIQELEHLLQTHGDQVHLLENDKKNLHTQIRTLQEIVDAKTVDLQNQQLVCGELRQNLDYENQKFNSENEKLQLQISELENAIKEQNCSASLDKSSYCENLLFQEKQLNSEMQKQMKELLLEKEDIQKCLNEAEVLHEQFVTGSRSHMESLQENISSKQNYIESIECAVREKEEQITTLTEKLGVLDANLQSVQEKNKELNDKLQELNLLSESWPLERERLNSQISLNREEIERLTEENKRTHQVNDPHNDQETNKESEHQLETTQPSAEDKVEPRGLPDGAGIENTNELEALQQELVHVKEKHCRLQREYDRLTNANEELTKLLDDLRNNEQSLDNITDLSAYLKEKENSLNNSNFVLETLPMDLDDEKQIHDGCTDSPTLKGTPNTVKSNNDLDLEQELSTLQKQDIFSFDIDQAERATNISDLLVNISDHQIGDRPSSSLDSSQCDDSINLNKDILGLRISGGWATTKPPQSISDNENSGLLTSPNEGTSKRLKETAENILKSPVPPSQRSSPSSMNSNNRGGREANRKSIPCKSAADSILGKSLEDQLWMMAVMASGDAGDFKEFLTTYQTEVDELKKQHLLEMEACKQKMTEQAAELEVKLAAEKQQTEYLSHELEAARLELQCLDLSARSFLSFDTEDLTKTLEVANQSICTVLPIGKLSLSSSELLTNHNLKNLIQSLESTNSETKKDRSILATNCPEIPNEGDENASSLEENSADSTNSNISDSPVSKLSLQEIMETLKMQIHQTTAENLKLLQSLEAGEKKNLNLSAEIESLNKQIEQHSGKDNIGTELQSRVKELDAEKLQLFEKYESISQEKLQLTGRVGDLEKELASISNAMEVLKEQLSQLSGIRESLEIANGNLKEQYLETENEVRRVKSERANMENHALSLEIDLDTIQAKYQQLQEENEEYRRSAATLQDRLSVVVAEKNQIDQELENLTEEKDELEQMCQKLKERVEDLELNKVHNRNLIKILETELRSLKAELQGAKASLEQLSAEKDLRDLQESEKNVHVEAEGLKNQLQQIQGEYQLLLKDSEDMQAQLSKVCSEKDKISKVLECCQYEKRELATNLSSAQEEVAQMRAGIEKLKVRMESDEKKKNHLIGKLKETERNSDHLKDKIENLERELLMSEENLESTILQSESSKEEVEKLKSMKEALEANVNTFRRRIVDLERELEKSKERIEELETRVLTLSNALEKSEMEKSCLNEESGQELLLLRAQLNELQEQKRASAKQSDLEALLEQNKMQLMQQLEEAHNTSRTLELTMEKVTLELRECKLQLDEKTELVLSLEGKLEEAENLEKKLAELAEQKALSDEKCNAAATKNAELLILIDQNKAELLHQVGEAQGETRNMELSLQKIATELGETKRRLEEKEQQIFVLECKLEETIESEKKCHLELLQCEAERDRLNGEIENQQRDLELLQCKVQMESGASESSQAAIRDLKSTCNDLEAQLQSSDAEKQVLVQKVDELTENCTILQSKVHEAEMKIKEKKVLDEQLQAVKQQIEDTNTRLCATTTEKTELTETIAKLRIEQEAQADRSRSDARELQNRLLQAEAKQQAALDALLIQHKEEIQALQGKLTLAEEHAIGHGYEVERLKAANVELNGSLGSAHRQLEELNQLKVSFAELKEEHARVCAALRHQVESCAELEQERDRLKGKINEREEELKHLQAKQQNAEGGASSNEDLLAEIEELKQWLEEKTTEADESVEKYCTIMIKSHKLEEDNDTLKKQLDFLSSKLKQLQTSQEVPSPPQRGDHPGPQSMDQKGGEDNQSAQRVSKQSSKRRREQDVTLDKEEPRSPIPQGLAKRVRKTASRTVPHQRSTEHEEEFQPDGLPDVVQKGFADIPSGKQSPYILRRTTGPVRKSPRLAALKPSPSTHEDLENLAHFSSPAAGGSKAQQSKLSEAVRAGSEVGPMEVSSPLSSYNRLKNPGSDSPISRKMAIERISEKLLHEESETEEACHVQ

Target ORF information:

RefSeq Version	XM_002934692.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis centromere protein F (cenpf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934692.5

ORF Insert Sequence:

ATGAGTTGGG TCGTAGAGGA GTGGAAGGAG GGGCTGCCCA CAAAGACCCT TCAGAAGATC 
CAGGAGCTGG AAAGTCAGTT GGACAAACTA AAGAAAGAGC GGCAGCAAAG GCAGTTTCAG 
CTGGAGTCGC TGGAAGCCGC TTTCCAAAAG CAGAAGCAAA AGGCAGAAAG TGAAAAAAGC 
GAAATCTCCG CCCTAAAACG AGAGAATCAG AGTTTGATAG AATCATGTGA CAATCAGGAG 
AAGGCAAAGC AGAAGATTAC CCATGAGTAC CAGCTAAAAG AGTCACAGGT CAACTTCCTG 
GAGGGCCAAC TCAGCGCCAG CAAGAAGCAA ATCGAAAAAC TGGAGCAAGA GCTTAAAAGG 
TACAAGAGTG ACTTGGAGAA AAGCCAACTG TCGTTCAATG CCTGTGACAT GTCTGTGTGC 
GCCACTCCCC AGAAGAGCTT TGTGGCGCCA TATACACCCA ATAAATACAC TGATTTCAGG 
TATGAAGAAT TGCAAGAGAA ATATCAGAAA GAGGTGGAAG AGAGGAAGAG GTTGGAAACA 
GAGATGAAAA CATTAAGGAA TGTGCCCCAG CCTCCACAGG CCGCTGCCCA GAGCACCATG 
ACGCACCGGG ATATCGCGCG GCACCAGACT TCCTCGTCTG TGTTCTCGTG GCAAGAAGAT 
CGAACCCCAA GTCACCCCTC ATCTACCAGT CATGATAAAA GACTTAAGAG AAGTTTTGCA 
TCATCCCAGC TCCTGTGGGA CCAGGAGGAA ACTCCAAGCA AAAGAGGTGT CCGATCAGAG 
AACTTGGGTA AAAGTGCCAG TGACTCCTAC ACCACTCAGC TTAATGATCA GCTAAAAACC 
CAGAATCAAG AGCTGAAATC CAGAATAAAT GAGCTGGAGA TGCGCTTGCA AGTTCAAGAG 
AAAGATCTGA AAAATCAGCT TAACAAACTC CAGGAAAGCC AAGCTCTGTA TGAAAAAACC 
CAAAGGGAAC TCGCAGAGAG AGACAAAACC CTGGTGAAAT GCAAGGAGGA CTTGGCACGT 
GTGACTGCGC AGTGTGAGCA GGCTGCTAAT AAGTGTGTGA TGGCGGAACA GAAGTTAAAG 
AAAGTTTCAG ATGAGCTGAG CTGCCAGAGG CAAAATGCTG AGAGTGCGAG GGTTGCTGCG 
GAGCAGAAAC TAAAGGAACG AGAGAAGGAA AACCAACAGG AGATTTTACA GCAGCAGAAT 
TCCCTAAGGA ATATGGAGCA GCAGCTCAGC CAGATAAAAG CCAAGATGAG CCAGGAGGCA 
CAGCAAGCCA AAAATGACTT CAACGCCCTC CAATCAGAGC TTGACCGGGC CATTCACACG 
AAAAAGATTT TGGAAAACGA AACAGAAGAA CTAAGGCAGA GGCTCAGCCG AGCGGAACAG 
GACTTGTGTC AGTGCCGAAG TAACGAAAAT GATTTAAGGA AGAGCCTTGA GGACACCAAG 
TCGGAAAAAA ATGTAATGAA AAGCCAGTAT GATCAGAAAT CTAAAGACGC TCTGAAACTG 
GAAGAAGAAT TACAAACGGC GAACCAAACC CTGAGGCAGA ACCAGCTCTT TGTGGATGAA 
ATAAAAGCTA AGAATCTTCA TTTAGAAGCA GAACTGAAGT CAGCTTTTGA GAAACTGCAA 
GACCACGACT CTGCCACCTT CCTGAATTTG AAAGCCACGG TGCATAATTT GGAAATGGAG 
AGGAATTTTG CCCAAGAATT ATTAAAGAAA AGAGAGAATG ATTTTGAAGA AATTAAAAAT 
AATTTAGCGA GAATGATGGA TGAAACAAAC TCATTAAAAA GTCACCTTGA TCACAAAGAA 
AAAGAATGTA AGGAACTGAT AAATGCTAAT ATTTCACTTA ATAGCTGGAA AGTGGAACAG 
GAGAAGGTGG TGGGTCAAAT TAGCAGAGAT AAAGATGATC TGCTGAAGAA GATTCAGGAG 
TTGGAACATT TGCTGCAGAC TCACGGTGAC CAAGTACACT TGTTAGAAAA TGACAAGAAA 
AACTTGCACA CTCAAATTAG AACCTTGCAA GAGATCGTCG ATGCCAAGAC CGTGGACCTG 
CAAAATCAGC AACTGGTATG CGGTGAACTT CGACAAAATC TGGACTACGA AAACCAGAAA 
TTTAATAGCG AAAATGAAAA GTTGCAGCTA CAAATTTCCG AGCTTGAGAA TGCAATCAAA 
GAGCAAAATT GTTCCGCTAG TTTGGACAAA TCGTCTTATT GCGAAAACTT GCTATTCCAG 
GAGAAGCAAT TGAATTCCGA AATGCAAAAA CAGATGAAAG AACTGTTATT AGAGAAGGAA 
GATATCCAGA AATGTCTAAA TGAAGCAGAG GTATTGCACG AACAGTTTGT CACAGGCTCC 
CGAAGCCACA TGGAGAGCTT GCAGGAAAAC ATTTCTTCCA AGCAGAACTA CATTGAGTCA 
ATAGAATGTG CAGTTAGAGA GAAAGAAGAG CAAATTACCA CTTTAACCGA AAAATTAGGA 
GTTCTAGATG CAAACTTGCA ATCTGTCCAG GAGAAAAACA AAGAGCTTAA TGATAAACTG 
CAGGAGCTCA ACCTGCTTTC TGAATCGTGG CCTTTAGAGA GAGAAAGACT AAATTCCCAG 
ATTAGTTTGA ATCGGGAGGA AATTGAACGG CTGACTGAAG AAAATAAAAG AACCCATCAA 
GTCAATGATC CTCACAATGA TCAGGAAACA AACAAAGAAT CGGAGCACCA GTTAGAGACT 
ACCCAGCCAT CGGCAGAAGA TAAAGTTGAG CCTAGAGGGT TGCCTGATGG GGCGGGTATT 
GAGAATACCA ATGAGCTGGA AGCACTTCAG CAAGAACTCG TTCATGTAAA AGAAAAACAC 
TGTAGACTGC AAAGAGAATA TGACAGACTC ACTAATGCCA ACGAGGAACT TACCAAGCTG 
CTTGATGACC TCCGAAACAA CGAACAGTCT CTCGATAACA TCACCGATTT AAGTGCTTAT 
TTGAAGGAAA AGGAAAATTC TCTAAATAAC AGTAATTTTG TGCTTGAGAC TCTTCCAATG 
GATCTTGATG ATGAGAAACA AATTCACGAT GGATGCACTG ATTCTCCTAC ACTAAAGGGT 
ACACCAAACA CCGTGAAGTC CAACAATGAT CTAGATTTAG AACAAGAGCT ATCTACACTT 
CAAAAACAGG ACATATTTTC ATTTGATATC GACCAGGCAG AACGTGCCAC CAATATTTCA 
GACCTTCTGG TGAACATCAG TGACCATCAA ATAGGTGATC GTCCATCCAG TAGTCTTGAT 
TCCAGCCAGT GCGATGACAG TATTAACCTT AATAAAGACA TTTTAGGCCT TCGAATATCT 
GGTGGTTGGG CAACTACAAA ACCACCTCAG TCTATTAGTG ACAACGAAAA TAGTGGTCTC 
TTGACTTCAC CGAACGAGGG CACATCAAAA CGTTTGAAAG AGACTGCAGA AAACATATTG 
AAATCACCAG TTCCTCCAAG TCAGCGTAGT AGTCCATCCT CAATGAATTC CAATAACAGA 
GGAGGCCGTG AAGCCAATAG AAAGTCTATC CCTTGCAAAT CTGCTGCTGA CTCCATTCTT 
GGGAAATCAC TCGAAGATCA GTTATGGATG ATGGCTGTTA TGGCCAGCGG AGATGCCGGA 
GACTTTAAAG AATTTCTTAC AACTTACCAA ACAGAAGTGG ACGAGTTAAA AAAACAGCAC 
CTTTTAGAAA TGGAGGCTTG CAAGCAGAAG ATGACGGAAC AGGCGGCAGA ACTGGAAGTT 
AAACTGGCAG CTGAGAAGCA ACAGACGGAG TATTTATCAC ATGAACTTGA AGCCGCCAGA 
CTTGAACTTC AGTGCCTTGA CCTGAGTGCA CGCTCATTCC TGTCTTTTGA TACTGAAGAT 
TTAACAAAAA CACTGGAAGT TGCAAACCAG AGTATCTGCA CCGTTTTGCC CATAGGAAAG 
TTATCTCTTA GCAGCAGTGA ATTGCTCACC AACCATAATC TAAAAAACCT CATACAAAGT 
CTAGAATCAA CCAACTCTGA GACCAAAAAG GACAGAAGCA TTCTGGCAAC TAACTGCCCG 
GAAATACCAA ATGAGGGAGA TGAAAATGCC TCAAGTCTCG AAGAAAACTC TGCCGACTCG 
ACAAATAGTA ACATTTCAGA TTCTCCCGTC AGTAAACTGA GTTTGCAAGA AATTATGGAA 
ACTCTAAAGA TGCAGATTCA TCAAACCACT GCTGAAAATC TTAAACTGCT TCAGTCGCTC 
GAGGCTGGAG AGAAGAAAAA TCTAAATTTG TCGGCAGAAA TAGAATCTCT TAATAAACAG 
ATTGAGCAGC ATTCTGGGAA GGACAATATC GGCACCGAGC TTCAAAGCAG AGTAAAAGAG 
CTTGACGCTG AAAAGCTGCA GTTGTTTGAA AAATATGAAT CGATTTCTCA AGAGAAACTG 
CAATTAACCG GTCGAGTTGG GGACCTTGAG AAGGAACTTG CTAGCATTTC TAATGCAATG 
GAGGTGCTGA AAGAACAACT GTCTCAATTG TCCGGCATAA GGGAGAGTTT AGAAATAGCA 
AACGGAAATC TAAAGGAACA GTATCTGGAG ACAGAAAATG AAGTAAGAAG GGTTAAATCA 
GAAAGAGCAA ACATGGAGAA CCATGCCTTA TCTCTTGAAA TAGATCTAGA CACAATTCAG 
GCTAAATACC AGCAGTTGCA AGAGGAAAAC GAAGAGTACC GAAGATCTGC TGCAACTTTA 
CAAGACCGTC TCAGTGTTGT TGTGGCAGAG AAGAATCAAA TTGATCAGGA GCTGGAGAAT 
TTAACTGAAG AGAAAGATGA GCTTGAGCAA ATGTGTCAGA AACTGAAGGA AAGGGTGGAA 
GACCTTGAAT TAAATAAAGT GCACAACAGA AATCTTATTA AGATATTGGA AACTGAATTG 
CGGTCTTTAA AAGCCGAACT TCAGGGAGCA AAAGCATCAT TGGAGCAGTT GTCTGCTGAA 
AAAGATCTAA GGGATCTGCA GGAATCTGAA AAGAATGTGC ATGTGGAAGC AGAAGGGCTG 
AAGAACCAGC TGCAACAAAT ACAAGGGGAG TATCAGCTTT TGTTAAAAGA CTCTGAAGAT 
ATGCAGGCCC AGCTGAGTAA AGTGTGCAGC GAAAAGGATA AAATCTCCAA AGTTCTAGAG 
TGTTGTCAAT ATGAGAAACG GGAATTGGCC ACAAACTTGA GCTCTGCTCA GGAAGAAGTT 
GCTCAGATGC GGGCTGGGAT TGAGAAGCTC AAGGTTCGAA TGGAGTCAGA TGAAAAGAAG 
AAGAATCACT TGATCGGGAA ACTAAAAGAG ACTGAAAGGA ATTCAGATCA TCTGAAAGAT 
AAAATCGAGA ATCTTGAACG GGAATTGCTT ATGTCGGAGG AAAATCTCGA GTCCACAATC 
CTTCAGTCGG AATCTTCTAA GGAAGAAGTA GAGAAACTAA AGTCGATGAA GGAGGCGCTA 
GAAGCTAACG TGAACACCTT CAGGAGGAGG ATTGTAGATC TAGAAAGAGA ACTTGAGAAA 
AGCAAAGAGA GGATAGAAGA ACTGGAAACC AGAGTACTTA CCCTTTCAAA TGCCCTGGAA 
AAGAGCGAAA TGGAGAAGAG TTGTCTCAAT GAGGAGTCCG GACAAGAACT TTTGTTGCTG 
CGGGCCCAGC TAAATGAGTT GCAAGAGCAA AAAAGAGCTT CTGCAAAACA GTCAGATCTT 
GAAGCTCTTC TGGAACAGAA TAAGATGCAG CTGATGCAGC AACTGGAGGA AGCACACAAT 
ACAAGCAGGA CCCTGGAATT AACCATGGAG AAAGTCACGT TGGAATTAAG GGAATGCAAA 
TTGCAACTTG ATGAAAAGAC TGAGCTGGTT CTGAGTCTAG AAGGCAAATT GGAAGAAGCA 
GAGAATTTGG AAAAGAAACT GGCTGAGCTA GCTGAGCAGA AGGCCCTTTC TGATGAGAAG 
TGCAATGCAG CTGCTACCAA GAATGCTGAG CTCCTTATTT TAATTGACCA AAATAAAGCA 
GAGCTGTTGC ACCAGGTAGG AGAAGCGCAA GGAGAGACCA GGAACATGGA ACTGTCTCTT 
CAGAAAATTG CTACAGAACT GGGTGAAACA AAGAGGAGAC TGGAAGAAAA GGAACAGCAG 
ATCTTTGTTC TAGAATGCAA GTTGGAAGAG ACCATTGAGT CTGAAAAGAA ATGCCATTTG 
GAACTGTTGC AGTGTGAAGC AGAAAGAGAC CGTCTAAACG GAGAAATTGA GAACCAGCAA 
AGAGATCTGG AGCTGCTACA ATGCAAGGTT CAGATGGAGT CTGGGGCCAG TGAGTCTTCT 
CAGGCAGCAA TCCGTGATCT GAAATCCACT TGCAACGATC TAGAAGCCCA GCTCCAATCT 
TCTGATGCGG AGAAGCAAGT TCTTGTGCAG AAAGTAGATG AGCTGACAGA AAACTGTACC 
ATCTTACAAA GCAAAGTACA TGAGGCGGAA ATGAAAATAA AGGAAAAGAA GGTGCTGGAT 
GAGCAACTGC AGGCAGTTAA GCAGCAAATT GAAGACACTA ATACACGGCT CTGTGCCACA 
ACCACAGAAA AAACTGAACT AACGGAGACG ATCGCCAAAC TCCGCATTGA GCAGGAGGCG 
CAGGCAGATC GGAGCCGCAG TGACGCAAGG GAATTGCAGA ACAGACTTCT TCAGGCCGAG 
GCTAAGCAGC AGGCTGCCCT GGATGCGCTA CTGATACAGC ACAAAGAGGA AATCCAGGCT 
TTGCAAGGCA AACTCACCTT GGCCGAAGAG CATGCGATTG GGCACGGATA TGAGGTTGAA 
CGGCTAAAAG CAGCTAATGT GGAACTGAAT GGCTCTCTGG GCAGTGCTCA CCGGCAACTG 
GAGGAGCTGA ACCAACTGAA GGTGAGTTTT GCTGAGCTGA AGGAAGAACA CGCGCGCGTC 
TGCGCTGCCC TCAGGCACCA GGTGGAGTCC TGCGCAGAAC TGGAGCAGGA GAGAGATCGG 
CTGAAGGGAA AGATAAACGA GAGAGAAGAG GAATTAAAAC ATCTTCAGGC AAAACAGCAA 
AATGCAGAGG GCGGAGCTAG CTCTAACGAG GACTTATTGG CTGAGATCGA GGAACTGAAA 
CAGTGGTTGG AGGAGAAGAC TACAGAGGCA GACGAGAGTG TTGAGAAGTA CTGCACGATA 
ATGATTAAAT CCCACAAACT GGAAGAGGAC AATGACACCC TGAAGAAGCA ACTCGACTTC 
CTCAGCTCCA AACTGAAGCA GCTGCAAACA AGTCAAGAGG TCCCAAGCCC ACCCCAGAGA 
GGGGACCATC CAGGACCACA GAGCATGGAC CAAAAAGGAG GAGAAGACAA CCAGTCGGCA 
CAGAGAGTAT CTAAACAGTC TTCCAAGAGG CGGCGAGAGC AAGATGTTAC TTTAGATAAA 
GAAGAGCCAC GATCTCCAAT ACCCCAGGGT TTGGCCAAAA GAGTTAGAAA GACTGCGAGC 
CGTACTGTGC CCCATCAGCG TTCCACTGAG CATGAGGAAG AGTTCCAACC TGATGGGCTC 
CCAGATGTTG TACAAAAAGG GTTTGCCGAT ATCCCATCAG GAAAACAAAG TCCTTACATT 
CTAAGGAGAA CGACAGGGCC CGTAAGGAAA AGCCCCCGAC TCGCTGCACT TAAACCTTCT 
CCATCCACCC ACGAAGATCT GGAAAACCTT GCGCATTTCT CCAGTCCAGC AGCAGGGGGC 
AGCAAAGCAC AGCAGTCGAA ACTCTCAGAA GCCGTTAGGG CTGGATCAGA AGTTGGACCC 
ATGGAAGTGT CTTCCCCACT CTCGTCCTAC AACAGACTAA AGAACCCGGG GTCTGACAGT 
CCGATCAGCA GGAAAATGGC AATAGAAAGG ATCTCGGAGA AGCTTCTGCA TGAAGAGTCT 
GAGACTGAGG AAGCCTGTCA TGTACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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cenpf cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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