CENPF cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	CENPF
Entrez Gene ID	533089
Full Name	centromere protein F
General protein information	Preferred Names centromere protein F Names centromere protein F mitosin
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005217369.4	XP_005217426.1	centromere protein F isoform X1
XM_005217369.3	XP_005217426.1	centromere protein F isoform X1
NM_001256586.1	NP_001243515.1	centromere protein F
XM_024976312.1	XP_024832080.1	centromere protein F isoform X2

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	CENPF	XP_001105956.2
Xenopus tropicalis (tropical clawed frog)	cenpf	XP_002934738.2
Homo sapiens (human)	CENPF	NP_057427.3
Pan troglodytes (chimpanzee)	CENPF	XP_001171564.1
Canis lupus familiaris (dog)	CENPF	XP_003639228.1
Mus musculus (house mouse)	Cenpf	NP_001074832.2
Bos taurus (cattle)	CENPF	NP_001243515.1
Gallus gallus (chicken)	CENPF	NP_989976.2
Rattus norvegicus (Norway rat)	Cenpf	NP_001094297.1

Gene Ontology
Bibliography

Related articles in PubMed

Silencing CENPF in bovine preimplantation embryo induces arrest at 8-cell stage.
Toralová T, Susor A, Nemcová L, Kepková K, Kanka J
Reproduction (Cambridge, England)138(5)783-91(2009 Nov)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Marçais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Unstable microtubule capture at kinetochores depleted of the centromere-associated protein CENP-F.
Bomont P, Maddox P, Shah JV, Desai AB, Cleveland DW
The EMBO journal24(22)3927-39(2005 Nov)

Silencing mitosin induces misaligned chromosomes, premature chromosome decondensation before anaphase onset, and mitotic cell death.
Yang Z, Guo J, Chen Q, Ding C, Du J, Zhu X
Molecular and cellular biology25(10)4062-74(2005 May)

Mitosin/CENP-F as a negative regulator of activating transcription factor-4.
Zhou X, Wang R, Fan L, Li Y, Ma L, Yang Z, Yu W, Jing N, Zhu X
The Journal of biological chemistry280(14)13973-7(2005 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CENPF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CENPF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa36388	NM_001256586.1 Latest version!	Bos taurus centromere protein F, 350/400kDa (CENPF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OBa223624	XM_024976312.1 Latest version!	Bos taurus centromere protein F (CENPF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OBa27521	XM_005217369.4 Latest version!	Bos taurus centromere protein F (CENPF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
OBa27521	XM_005217369.3	Bos taurus centromere protein F (CENPF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CENPF ( NM_001256586.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001243515.1 Bos taurus centromere protein F, 350/400kDa (CENPF), mRNA.

Price & Availability↑

CloneID	OBa36388
Clone ID Related Accession (Same CDS sequence)	NM_001256586.1
Accession Version	NM_001256586.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1420214400000
Organism	Bos taurus(cattle)
Product	centromere protein F
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAFC03017426.1, AAFC03031658.1 and AAFC03031660.1. On or before Feb 12, 2012 this sequence version replaced XM_612376.5, XM_002694283.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN02403656 [ECO:0000350] ##Evidence-Data-END##

ATGAGCTGGG CTTTGGAAGA ATGGAAGGAA GGCTTGCCTA CAAGAGCTCT TCAGAAGATT 
CAAGAGCTTG AAGGCCAGCT CGACAAACTG AAGAAAGAAA GGCAGCAACG GCAGTTTCAG 
CTTGAGACCC TGGAGGCTGC GTTGCAGAAG CAAAAACAGA AGGTTGAGAA TGAAAAAACT 
GAGGGTGCAA ACCTGAAAAG GGAGAACCAA AGCTTGATGG AAATATGTGA AAATCTGGAG 
AAGACTAAGC AGAAGATTTC TCATGAACTT CAAGTCAAGG AGTCACAAGT GAATTTCCAA 
GAAGGTCAAC TGAACTCAAG CAAAAAACAA ATAGAAAAGC TGGAACAGGA ACTTAAAAGG 
TGTAAATCTG AACTTGAAAG AAGCCAACAA GCTGCACAGT CTGCGGATGT CTCTCTGAAT 
TCATGCCTTA CACCACAAAA AATCTTTGCA ACTCCGCTCA CACCAAGCCA GTATTATAGC 
GGTTCCAAAT ATGAAGATCT AAAAGAAAAA TATAATAAAG AAGTTGAAGA ACGAAAAAGA 
TTAGAGGCAG AGGTAAAAGC CTTGCAGGCA AAGAAAGCAA GCCAGACTAT TCCCCAAAGC 
ACCATGAATC ACCGGGACAT CGCCCGACAT CAGGCTTCTT CATCTGTGTT CTCATGGCAA 
CAAGAAAAAA CCCCAAGCCG TCTTTCATCT AACACTCTGA AAACTCCAGT TAGGAGAGAT 
TTCTCTGCAT CTCATTTTTC TGGGGAACAA GAAGTGACTC CGAGTAGATC AACTTTGCAA 
ATAGGGAAAA CAGATGCTAA TAGCAGTTTC TGTGATAATT CTAGCAATTC TCATCTTTTG 
GACCAGTTAA AAGCACAGAA CCAAGAACTA AGGAGCAAGA TGAGTGAGCT GGAACTACGT 
CTGCAAGGAC AAGAAAAAGA AATGAAAGGC CAAGTGAATA AACTTCAAGA ACTCCAACTG 
CAACTGGAGA AAGCAAAAGT GGAATTAAAT GAAAAAGAGA AAGTTTTGAA CAAAAACCGG 
GATGAACTAG TGAGAACAAC TTCACAGTAT GACCAGGCAT CAGCCAAGTG TACTGCTTTG 
GAACAAAAAT TGAAAAAATT GACTGAAGAT TTGAGTTGTC AGCGTCAAAA TGCAGAAAGT 
GCCAGATGTT CTCTGGAACA GAAAATCAAG GAGAAAGAAA AAGAGTTCCA AGAGGAGCTC 
TCCCGTCAAC AGCGTTCCTT CCAAACCCTG GACCAGGAGT GCACTCAGAT GAAAGCCAGG 
CTCACCCAGG AGCTACAGCA AGCTAAGAAC ACATACAACA TCCTACAGGC TGAACTGGAT 
AAAGTCACAT CTGTAAAGCA ACAGATAGAA AAAAAATTGG AAGAGTTTAA GCAAAATTTC 
AGCAAAACTG AACAGGCCTT ACAGGCAAGT CAGACCAAGG AAAATGAGCT GAGGAGAAGC 
AGTGAGGAAA TGAAGAGGGA AAACAGCCTC CTCAAGAGTC AGTCTGAGCA AAGGGCCCGA 
GAAGTCTGCC ACCTGGAGGA AGAACTGAAG AAGGCCAAGC AGTGTTTGAG TCAGAGCCAG 
AATTTTGCAG AAGAAATGAA GGCTAAGAAT ACCTCTCAGG AAACCATGTT AAGAGATCTT 
CAAGAAAAGA TAAATCAGCA AGAGAATTCC TTGACTTTAG AGAAACTGAA GCTTGCCTTG 
GCTGACCTGG AAAAGCAGCG AGATTGTTCT CAAGACCTTT TGAAGAAAAG GGAACATCAC 
ATTGAACAAC TGAACGAAAA GTTAAGCAAA ACAGAGAGAG AATCTGAAGC ATTGTTGAGT 
GCTTTGGAAT TAAAGAAGAA AGAATATGAA GAATTAAAAG AAGAGAAAAC TCTGTTTTCT 
CGCTGGAAAA GTGAAAACGA ACAACTTTTA AATCAGATGG AATCAGAAAA AGAAAGCTTG 
CAGAGTAAAG TTAATCACTT GGAAACTTGT CTTAAGACAC AACAAATAAA AAGTCATGAA 
TATAATGAGA GGGTAAGAAC TCTGGAGATG GAAAGAGAAA ATCTAAACGT GGAGATCAGG 
AACCTTCAAA ATGTGATTGA CAGCAAAACC GCAGAGGCAG AGACACAGAA ACGAGCTTAT 
GGAGAACTGC AACAGAAAGC TGAGTTCTCA GATCAGAAAC ACGAGAAGGA GATAGAAAAT 
ATGTGTTTGA AAATCTCTCA CCTCACTGGG CAAGTGGAAG ACCTAGAACA TAAGCTTCAG 
TTACTGTCAA GTGAAATCAT GGACAAAGAC CAGCGTTACC AAGACTTGCA TGCTGAATCT 
GAGAGCCTCA GGGATCTGCT AAAATCCAGA GATTCCTCTG CGATGACAAT TGAGCCTCAT 
CAGAGAAGTC GTTTAGCTTT TGAACAGCAG TCTGCACTGA ACAATTCCTT TGCAAATATA 
ATCGGAGAGC AAGAAAGTGT GCCTTCAGAA AGGAGCAGAT GCCATGTAGC AACAGACCAA 
AGTCCGAAAA GTTCATCCAT GTTGCAAAAT AGAGTTGTTT CTCTTGAATT TTCCTTAGAG 
TCGCAAAAGC AGATGAACTC AGATCTGCAG AAGCAGTGTG AAGAGTTGGT GCAAATCAAA 
GGAGAAATTG AAGAAAACCT CATGAAAGCA GAACAGATGC ATCAAAGTTT TGTGGCTGAA 
ACAAGCCAAC GCATTAGTAA GTTACAAGAA GACACTTCTG TTCATCAGAA TGTTGTTGCT 
GAAACTTTAG CTGCCCTGGA GAGCAAGGAA AGAGAGTTGC AACTTTTGAA TGAAAAATTA 
GAAACTGAGC AGGCAGAGAC TGGAGAGTTA AAAAAGAACA ACCATTTACT TCAGGAATCT 
CTAAAGGAGC TACAGTTTTT GTCTGAAACC CTGAGCTTGG AGAAAAAGGA ACTGAATTCC 
ATCATTTCTC TAAATAAAAA GGACATTGAA GAGTTGACCC AAGAGAATGG GACTCTCAAG 
GAAATTAATG CCACCTTAAC TCAAGAGAAG ATGAACTTGC TCCAAAAAAC CGAGAGTTTT 
TCGAACTGTA TAGATGAAAG AGACAGAAGC ATTTCAGAGT TATCTAATCA GTACAAACAA 
GAAAGGCTTA CTTTACTACA AAGATGTGAA GAAACAGGAA ATGCATTTCA GGATCTTAGT 
GAAAAATATA AAGCGGTTCA GGAAAAGAAC TCTAAATTAG AATGCTTGCT GAGTGAGTGC 
ACGGGTGTTT GTGAAGACAG AAAAAATGAA TTAGGACAGC TAAAGGAAAC ATTTGCAAGG 
GAACACCAAG CATTTGTATC ACAGTTAGCA TTAGCTGAAG AAAGAAACCA GAATTTAATT 
GTGGAGTTGG AGACAGTGCA GCAAGCCCTG CAGTCTGAGA TTACAGATAT CCAAAACAGC 
TCCAAGAGGG AGACTGATGG TTTAAAGCAA GAAATCATGA ATTTAAAGGA AGAACAAAAT 
AAGATGCAAC AGGAAGTTAG TGCCTTATTA CAAGAGAATG AACACCTGAT GGAATTAATG 
AAGACGAAAC ATGAGCATCA ATGTCTAGAA TTGGAACCAA TTCAAGACTC TGAGAAAGAA 
GAGAGGGAGA TAAATACATG TCACCTTCAA CTTCCGATGG ATCTTGATGT TAAAGATACT 
TCTCCAGACA CTTGTAATGC ACAGCTGGTG CAAGTAGAAT CTAAAGTCAG AAACATGGAA 
TTAAAACTTC AGAAGAGTGA GAAGGAAGAG TGTCTACAGT ATGAGTTACA AACTAGAGAA 
TTAGAAACTG AAGATTTGCA ACAAGATCCT CAGTCACAAG ATATTAGTGG CCTTGGAGAC 
TCTGAAATAG ATCCAGAAGA AAAATATATT TCAGTGCTTC AGGAGTTGTC AACAAGTCAG 
CAGGACAATG CCCACCTGCA GTGTTCTCTA CAGACAGCCA TGAACAAGCT GAATGAGTTA 
GAGAAAATGT GTGAAGCCCT GCAGGTTGAA AAGTCTGAAC TCATATCTGA GCTGAATGAC 
TCAAGATCAG AATGTATCAC AGCAACTAGT AAAATGGCAG AAGAGGTAGG GAAACTAGTA 
AATGAAGTTG AAACATTAAA TGACGAAAAT GCACTTCTCC AAGGCGAGTT AGTGGAAGAA 
ACACCAGAAG GTGAATTTGG TGAACAACAA GATGAGCAGA CATCTGTGAA CTTAAATCCT 
TTGGATGACA GTAATTTCTA TGAGCATTTG ACCATGTCAA GCAAAGAGGT TCAAATGCAC 
TTTGCTGAAT TACAGGAGAA ATTTTCATCT TTACAGAGTG AGCACAAAAT TTTACACGAA 
CAGCACTGTC TGATGAGCTC TAAGATGTCA GAGCTGCAGT CCTACGTCGA CACGTTAAAG 
GCTGAAAATT CAGTCTTGTC GATGAGTCTG AGAAGCTCTC AAGGTGACTT GGTAAAGGAG 
GTGCCACCAG GACCTGGGGA GGAGCATTTA CTGTCCTTGT CATTTTCTTG TGTGACTGAC 
AGCCCTGGTA AGGCAAGTTT GGGAGAGTCC TCTTTTTACA AAGATCTTCT AGAGCACACA 
ACAGAAACGT CCCTTTTGAA TAATCTAGAA GGGACTGTTT CGGCAAACCA GTCCAATGTA 
GAGGAAGTCT CTTGTAGTAG TCTGGAGGAG GAGAATCTGA CCGAGAAAGA GATCCCCTCT 
GTCCCACTGA GGAGCGCTCG AGAACTTGAG ACCCTCTGTC AGACGTACCT CGAGTCCCTC 
AAGCAGCTGG AAGAGGAAAT CGAAAGTCAA GGGATTACGA AAAACAAGGA GATCAAAGAG 
CTCAAGGAGC TACTGAGTTC TTCGAGGGAG GAGCTTGACG ACCTCAGGAA GCAGTATTTG 
TCAGAAAATG AACAGTGGCA GCAGAAGCTG ACAGATGTGA CAGCGGAGAT GGAGTCCAAG 
TTGGCGGCTG AAAAGAGACA CGCAGAGCAC CTTACACTTG AGCTTGAGGT GGCGCGGCTT 
CAGCTGCAGG GTCTGGACTT AAGCTCTCGG TCTCTGCTTG GCACCGACAT AGAAGATGCT 
ATTCTGGGTG GAAACGATAG CTGTGACATA AGAGAATCAG AAGAATATAT TTCAGAAACT 
AAAGAGAGGA CCCCAAAGCA TGAGATTCAC CAGATTTGTG AAAAAGATGT TCAGCAGGAC 
CTCAGTCTAG AGATGGAGAA AATAACAAAG ACTGGTGCAA CCAAACTTAC AGAAGAATGG 
TCCAGAGAGC AGTCCTCAGA AAGCAGTCAT GAGACCCCAG TGGAAGATCC AGCCCAGGGC 
TGTTCAGGAT GCATTTCTGA ACTGTCCCTT TCTGGGCCTA ATGCTTCAGT GCCTAGGGAT 
TTTCTGGAGA ATCAGGTAAC CATTCAGAGT CTCGAGCTGA AAGTAAAAGA GACATCAAAT 
GGGAATTTGA GATTACTTCA TGGCATAGAG GAGCGTGACC AAAAAGTTGC CAACTTGCTA 
AATGAAATCA AAGAGTTAGA CTCAAAATTC CATTTACTGG AAGTACAATT AACTACCAAG 
ATTGAAGCAT GTGTGGCATT GGAAAAAATA GTTGAGGAAC TTAAGAAAGA AAAATTAGAT 
TTAAATGAAA AATTGGAATC CTTTTCTTGT CATAACCAGA GAGAAGAAAG TTCAGGAGGT 
CTCACTTCCA ACTTAGAAAT GGTCACAAGC AAATTTCCAC ATGAAGGTAT TGAAGATGAT 
GTAGCCAAGG TGACTGACAA CTGGAGAGAG AAATGTCTCC AGGTGGAAAA TGAGCTACAG 
AGGATTCAAT CTGAGAAAGA TAGCATGGAG CATCATGCCC TCTCTGCTGA AGCCAGCTTA 
GAGGCAGTTC AAACAGAGAA ACTATATTTA GAAAAAGACA ATGAAAATAA ACAAACAGTT 
ATAACCTGTC TTGAGGAAGG ACTCTCAGTG GTCACAAGTG AGAGAGATCA GCTTCGTGGA 
GAGTTAGATA CTTTGTCAAA AGAAAATCAA GAGCTAGATC AGATGTCTGA AAAGATGAAG 
GAGAAAATAC GGGAGCTTGA ATCTCATCAA AGTGAGTGTC TGCATCTCCA AGAGTGGCTT 
CAGAGTTTGG AAAAGGACTC ACAGGCACTG TCTCTGGTAA GAAGTGAGCT GGAAAACCAA 
ATTGAACAAC TGAATAAAGA GAAAGACTCA CTTGTATGGG AATCCGAAAG CCTGCAGACC 
AAACTGAGCG AATCGGAGCA TGAAAAGCTG GCCATCACCA AGGCTTTGGA GGCTGCCCTG 
ATGGAGAAGG GTGAGGTCGC GGTGAGGCTG AGCTCCACGC AAGAGGAAGT GCACCAGTTG 
AGAAAAGGCA TTGAGAAACT CAGGGTCCGC ATTGAGGCTG ATGAAAAGAA GCAGCTCCAT 
GTCTCAGAGA AACTGAAAGA AAGCGAGCGG AGGAATGACT CGCTTCAGGA CAAGGTTGAG 
ACCCTTGAAA GGGAGTTGCA GATGGCAGAA GAAAACCAAG AGCTGGTGAT TCTTGATGCT 
GAGAATTGCA AAGCAGAGGT GGAAACCCTA AAAACACAAA TAGAATTGAT GACTGAACGC 
CTAAAAGATT TAGAATTAGA CCTTGTCACA ATACGGTCTG AAAAAGAAAA TCTTTTGAAA 
CAGCTACAAG AGAAACAAGG TCAGGCGTCT GAATTAGATA CGCTACTCTC TTCATTGAAA 
AACCTATTAG AAGAAAAGGA GCGAGAGAAA ATACAGACGA AAGAAGAAGC TAAAAGTGCA 
GTGGAGATGC TGCAAACACA GTTGAGAGAG CTAACTGAGG AAATAGCAGC CTTATGTGAT 
GACCAGGAGA CATGGAAGGT CGAAGAACAG AGTCTGGACT CTCCTGCCCA GGAGGTACAA 
CAGCTGAGAA ATAACATTGA AAAGCTGAAA GTCCACTTGG ACATTGATAA AAAGAAGCAG 
CTCCAAATCC TAGAAAAACT GAAGGAAAGT GAGCATCAGG CCGATTTCCT TAAGGATACA 
GTTGAGAATC TTGAAAGAGA ACTCAAGCTA TCAGGAGAAA ACCAAGAGCA TGTGACTCTT 
GAGGCTGAGA AGTCCAAAGC AGAAGTAGAG ACCCTGAAAG CAACAGTGGA GAAGATGGAC 
CAAAATCTGA GAGGTTTGCA ATTAGATCTT GTCAATATAA GATCAGAAAA AGAAGATCTG 
ACTAAAGAAT TACAGAAAGA GCAGAGTCGA GTATCTGAAT TAGAAACGTT AAATTCTTCA 
TTTGAAAACC TATTGCGGGA AAAAGAGCAA GAAAAAGTAC AGATGAAGGA AGAATCTAAA 
GCTGCAGTGG AGATGCTGCA AACACAGCTG AAAGAGCTAA AGCTAAACGA GAAAATGGCA 
GCCTTGGGTA ATGACCAAGA GACCCGGAAG GTCAAAGAAC AGAGCCTGAG TAGTCAAGTA 
GATTCCCTTG AACATGAGAA GGCTCAGTTG CTGCAGGACC TTGATGATGC CAAAAGTAAT 
TACATGATTT TGCAGTCTTC TGTGAATGAC CTCATTCAAG AAGTTGAAGA TGGCAAACAG 
AAACTAGAGA AAAAGGATGA AGAAATCAGT ATACTAAAAA GTCAGACTCG GGACCAAGAG 
CAGCTTGTCT CTAAACTGTC CCAGATGGAA GGAGAACAGC AACTTTGGAA GAAGCAAAAG 
GCAGACCTGG AAAATCTGAT GGTGGAATTG GAGCAGAAGA TTCAGGTGCT GCAATCCAAA 
AACGATGCTT TGCAGGACAC CTTAGAAGCT CTGCAGAATT CTTCCAGGAA TCTGGAGAAA 
GAGCTTGAAT TGACAAAATT GGAAAAAATG TCCTTTGTTG AAAAAGTAAA CACAATGACT 
GTGAAGGAAA CTGAGCTTCA GAAGGAAATA CATGCAGTGA TACAAAAAAC GGTGGCACTG 
AAAGAAGAAT TTAGTGGGGA GAAAAACAGG CTAATGGAAG AATTAAATTT AATGTTGGAA 
GAAGTGAAGA GCAGCAAAGG TCAACTGAAG GAGCTCATGC TAGAAAATAG TGAATTGAAG 
AAGAGTTTGG ATTGTGTACA CAAAGACCGT ATGGAAGAAC AAGGGAAAAT GAGAGGAGAA 
ATAGCTGAAT ATCAGCTACG GCTTCAGGAA GCTGAAAACA AACATCAGGC TTTGCTTCTA 
GATACAAACA AACAGCATGA AATGGAAATC CAGACATACC GAGAGAAACT GACTTCTAAG 
GAGGAATGTC TCAGCTCACA GAAGGTGGAG ATAGACCTCT TAAAGTCCAG TAAAGAAGAA 
CTCAATAATT CTTTGAAAGC GACCACTGAG ATTTTAGAAG AATTGAAGAA AACCAAGATG 
GAAAATCTAA AACATGCAGA TAAATTGAAG AAGGAAAACG ATCGTGCCCA GAGTAAAATA 
AAGTTGTTGG TGAAATCCTG TAAACAGCTG GAAGAGGAAA AGGTGATGTT GCAGAAAGAA 
CTATCTCATC TTGAAGCTGC ACAGGAGAAA CAGAGAGCAG ATACCGTCGT GGATGCTAAT 
GTCGACGAAT TAATAACTGA AATGAAAGAA CTGAAAGAAA CCCTTGAAGA AAAAACCAAG 
GAGGCAGACG AATACCTGGA TAAATACTGT TCCCTGCTCA TAAGCCATGA GAAGTTAGAG 
AAAGCCAAGG AGATGTTAGA AACACAAGTT GCTCGTCTGA GTTCACAACA ATCTAAACTG 
AATCTTCGGA GATCTCCTTT GGTGAATTCG CTTGCTCCAG GACCATCCCC AGTCCCTTCT 
GCCACTGAGA AGAAGTTACC ATCCGGCCAA AATAAATCTT CAGGCAAAAG GCAAAGGTCC 
AGTGGGATTC GGGAGGATGG CGGAGAAACC ACGCCTTCTA CACCAGAGAC ATTTTCTAAA 
AAAAGCAGGA AAGCCATCAG AAGTGGTACT CACCTTGCAG AGGATGCAGA AGATGCTGAA 
TTTGAGCCAG AGGGGCTTCC AGAAGTTGTA AAGAAAGGGT TTGCCGACAT CCCAACCGGA 
AAGACTAGCC CCTACATCCT GCGGAGGACA ACCATGGCAA CCAGGACCAG CCCCCGCCTT 
GCTGCCCAGA AGTTAGCAGC ATCCCCGCCA AGTCTCGACA AAGAAAATCT CACCGAGACC 
TCCAAACCAA CAGCTGGTGG CAGCAGATCA CAAAAGGTCA AGGTTTCTCA ACAGAGCCCA 
GTGGATTCAG GCACTGCCTT CCGAGATCCC ATTGGCAGAT CTCTCTCCAT CAGTAATCTT 
CCCGAGAGAA GTCCAGTGGG CAGCCCCAGG GAGGGCCTGA GAGCCAAGCG AGGCCGCCAC 
GCCCCCAGCC CAGAATCCAG CCTGGAGTCC AAGGGCAGCG AGAACTGTAG GGTCCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243515.1
CDS	148..9387
Translation	MSWALEEWKEGLPTRALQKIQELEGQLDKLKKERQQRQFQLETLEAALQKQKQKVENEKTEGANLKRENQSLMEICENLEKTKQKISHELQVKESQVNFQEGQLNSSKKQIEKLEQELKRCKSELERSQQAAQSADVSLNSCLTPQKIFATPLTPSQYYSGSKYEDLKEKYNKEVEERKRLEAEVKALQAKKASQTIPQSTMNHRDIARHQASSSVFSWQQEKTPSRLSSNTLKTPVRRDFSASHFSGEQEVTPSRSTLQIGKTDANSSFCDNSSNSHLLDQLKAQNQELRSKMSELELRLQGQEKEMKGQVNKLQELQLQLEKAKVELNEKEKVLNKNRDELVRTTSQYDQASAKCTALEQKLKKLTEDLSCQRQNAESARCSLEQKIKEKEKEFQEELSRQQRSFQTLDQECTQMKARLTQELQQAKNTYNILQAELDKVTSVKQQIEKKLEEFKQNFSKTEQALQASQTKENELRRSSEEMKRENSLLKSQSEQRAREVCHLEEELKKAKQCLSQSQNFAEEMKAKNTSQETMLRDLQEKINQQENSLTLEKLKLALADLEKQRDCSQDLLKKREHHIEQLNEKLSKTERESEALLSALELKKKEYEELKEEKTLFSRWKSENEQLLNQMESEKESLQSKVNHLETCLKTQQIKSHEYNERVRTLEMERENLNVEIRNLQNVIDSKTAEAETQKRAYGELQQKAEFSDQKHEKEIENMCLKISHLTGQVEDLEHKLQLLSSEIMDKDQRYQDLHAESESLRDLLKSRDSSAMTIEPHQRSRLAFEQQSALNNSFANIIGEQESVPSERSRCHVATDQSPKSSSMLQNRVVSLEFSLESQKQMNSDLQKQCEELVQIKGEIEENLMKAEQMHQSFVAETSQRISKLQEDTSVHQNVVAETLAALESKERELQLLNEKLETEQAETGELKKNNHLLQESLKELQFLSETLSLEKKELNSIISLNKKDIEELTQENGTLKEINATLTQEKMNLLQKTESFSNCIDERDRSISELSNQYKQERLTLLQRCEETGNAFQDLSEKYKAVQEKNSKLECLLSECTGVCEDRKNELGQLKETFAREHQAFVSQLALAEERNQNLIVELETVQQALQSEITDIQNSSKRETDGLKQEIMNLKEEQNKMQQEVSALLQENEHLMELMKTKHEHQCLELEPIQDSEKEEREINTCHLQLPMDLDVKDTSPDTCNAQLVQVESKVRNMELKLQKSEKEECLQYELQTRELETEDLQQDPQSQDISGLGDSEIDPEEKYISVLQELSTSQQDNAHLQCSLQTAMNKLNELEKMCEALQVEKSELISELNDSRSECITATSKMAEEVGKLVNEVETLNDENALLQGELVEETPEGEFGEQQDEQTSVNLNPLDDSNFYEHLTMSSKEVQMHFAELQEKFSSLQSEHKILHEQHCLMSSKMSELQSYVDTLKAENSVLSMSLRSSQGDLVKEVPPGPGEEHLLSLSFSCVTDSPGKASLGESSFYKDLLEHTTETSLLNNLEGTVSANQSNVEEVSCSSLEEENLTEKEIPSVPLRSARELETLCQTYLESLKQLEEEIESQGITKNKEIKELKELLSSSREELDDLRKQYLSENEQWQQKLTDVTAEMESKLAAEKRHAEHLTLELEVARLQLQGLDLSSRSLLGTDIEDAILGGNDSCDIRESEEYISETKERTPKHEIHQICEKDVQQDLSLEMEKITKTGATKLTEEWSREQSSESSHETPVEDPAQGCSGCISELSLSGPNASVPRDFLENQVTIQSLELKVKETSNGNLRLLHGIEERDQKVANLLNEIKELDSKFHLLEVQLTTKIEACVALEKIVEELKKEKLDLNEKLESFSCHNQREESSGGLTSNLEMVTSKFPHEGIEDDVAKVTDNWREKCLQVENELQRIQSEKDSMEHHALSAEASLEAVQTEKLYLEKDNENKQTVITCLEEGLSVVTSERDQLRGELDTLSKENQELDQMSEKMKEKIRELESHQSECLHLQEWLQSLEKDSQALSLVRSELENQIEQLNKEKDSLVWESESLQTKLSESEHEKLAITKALEAALMEKGEVAVRLSSTQEEVHQLRKGIEKLRVRIEADEKKQLHVSEKLKESERRNDSLQDKVETLERELQMAEENQELVILDAENCKAEVETLKTQIELMTERLKDLELDLVTIRSEKENLLKQLQEKQGQASELDTLLSSLKNLLEEKEREKIQTKEEAKSAVEMLQTQLRELTEEIAALCDDQETWKVEEQSLDSPAQEVQQLRNNIEKLKVHLDIDKKKQLQILEKLKESEHQADFLKDTVENLERELKLSGENQEHVTLEAEKSKAEVETLKATVEKMDQNLRGLQLDLVNIRSEKEDLTKELQKEQSRVSELETLNSSFENLLREKEQEKVQMKEESKAAVEMLQTQLKELKLNEKMAALGNDQETRKVKEQSLSSQVDSLEHEKAQLLQDLDDAKSNYMILQSSVNDLIQEVEDGKQKLEKKDEEISILKSQTRDQEQLVSKLSQMEGEQQLWKKQKADLENLMVELEQKIQVLQSKNDALQDTLEALQNSSRNLEKELELTKLEKMSFVEKVNTMTVKETELQKEIHAVIQKTVALKEEFSGEKNRLMEELNLMLEEVKSSKGQLKELMLENSELKKSLDCVHKDRMEEQGKMRGEIAEYQLRLQEAENKHQALLLDTNKQHEMEIQTYREKLTSKEECLSSQKVEIDLLKSSKEELNNSLKATTEILEELKKTKMENLKHADKLKKENDRAQSKIKLLVKSCKQLEEEKVMLQKELSHLEAAQEKQRADTVVDANVDELITEMKELKETLEEKTKEADEYLDKYCSLLISHEKLEKAKEMLETQVARLSSQQSKLNLRRSPLVNSLAPGPSPVPSATEKKLPSGQNKSSGKRQRSSGIREDGGETTPSTPETFSKKSRKAIRSGTHLAEDAEDAEFEPEGLPEVVKKGFADIPTGKTSPYILRRTTMATRTSPRLAAQKLAASPPSLDKENLTETSKPTAGGSRSQKVKVSQQSPVDSGTAFRDPIGRSLSISNLPERSPVGSPREGLRAKRGRHAPSPESSLESKGSENCRVQ

Target ORF information:

RefSeq Version	NM_001256586.1
Organism	Bos taurus(cattle)
Definition	Bos taurus centromere protein F, 350/400kDa (CENPF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256586.1

ORF Insert Sequence:

ATGAGCTGGG CTTTGGAAGA ATGGAAGGAA GGCTTGCCTA CAAGAGCTCT TCAGAAGATT 
CAAGAGCTTG AAGGCCAGCT CGACAAACTG AAGAAAGAAA GGCAGCAACG GCAGTTTCAG 
CTTGAGACCC TGGAGGCTGC GTTGCAGAAG CAAAAACAGA AGGTTGAGAA TGAAAAAACT 
GAGGGTGCAA ACCTGAAAAG GGAGAACCAA AGCTTGATGG AAATATGTGA AAATCTGGAG 
AAGACTAAGC AGAAGATTTC TCATGAACTT CAAGTCAAGG AGTCACAAGT GAATTTCCAA 
GAAGGTCAAC TGAACTCAAG CAAAAAACAA ATAGAAAAGC TGGAACAGGA ACTTAAAAGG 
TGTAAATCTG AACTTGAAAG AAGCCAACAA GCTGCACAGT CTGCGGATGT CTCTCTGAAT 
TCATGCCTTA CACCACAAAA AATCTTTGCA ACTCCGCTCA CACCAAGCCA GTATTATAGC 
GGTTCCAAAT ATGAAGATCT AAAAGAAAAA TATAATAAAG AAGTTGAAGA ACGAAAAAGA 
TTAGAGGCAG AGGTAAAAGC CTTGCAGGCA AAGAAAGCAA GCCAGACTAT TCCCCAAAGC 
ACCATGAATC ACCGGGACAT CGCCCGACAT CAGGCTTCTT CATCTGTGTT CTCATGGCAA 
CAAGAAAAAA CCCCAAGCCG TCTTTCATCT AACACTCTGA AAACTCCAGT TAGGAGAGAT 
TTCTCTGCAT CTCATTTTTC TGGGGAACAA GAAGTGACTC CGAGTAGATC AACTTTGCAA 
ATAGGGAAAA CAGATGCTAA TAGCAGTTTC TGTGATAATT CTAGCAATTC TCATCTTTTG 
GACCAGTTAA AAGCACAGAA CCAAGAACTA AGGAGCAAGA TGAGTGAGCT GGAACTACGT 
CTGCAAGGAC AAGAAAAAGA AATGAAAGGC CAAGTGAATA AACTTCAAGA ACTCCAACTG 
CAACTGGAGA AAGCAAAAGT GGAATTAAAT GAAAAAGAGA AAGTTTTGAA CAAAAACCGG 
GATGAACTAG TGAGAACAAC TTCACAGTAT GACCAGGCAT CAGCCAAGTG TACTGCTTTG 
GAACAAAAAT TGAAAAAATT GACTGAAGAT TTGAGTTGTC AGCGTCAAAA TGCAGAAAGT 
GCCAGATGTT CTCTGGAACA GAAAATCAAG GAGAAAGAAA AAGAGTTCCA AGAGGAGCTC 
TCCCGTCAAC AGCGTTCCTT CCAAACCCTG GACCAGGAGT GCACTCAGAT GAAAGCCAGG 
CTCACCCAGG AGCTACAGCA AGCTAAGAAC ACATACAACA TCCTACAGGC TGAACTGGAT 
AAAGTCACAT CTGTAAAGCA ACAGATAGAA AAAAAATTGG AAGAGTTTAA GCAAAATTTC 
AGCAAAACTG AACAGGCCTT ACAGGCAAGT CAGACCAAGG AAAATGAGCT GAGGAGAAGC 
AGTGAGGAAA TGAAGAGGGA AAACAGCCTC CTCAAGAGTC AGTCTGAGCA AAGGGCCCGA 
GAAGTCTGCC ACCTGGAGGA AGAACTGAAG AAGGCCAAGC AGTGTTTGAG TCAGAGCCAG 
AATTTTGCAG AAGAAATGAA GGCTAAGAAT ACCTCTCAGG AAACCATGTT AAGAGATCTT 
CAAGAAAAGA TAAATCAGCA AGAGAATTCC TTGACTTTAG AGAAACTGAA GCTTGCCTTG 
GCTGACCTGG AAAAGCAGCG AGATTGTTCT CAAGACCTTT TGAAGAAAAG GGAACATCAC 
ATTGAACAAC TGAACGAAAA GTTAAGCAAA ACAGAGAGAG AATCTGAAGC ATTGTTGAGT 
GCTTTGGAAT TAAAGAAGAA AGAATATGAA GAATTAAAAG AAGAGAAAAC TCTGTTTTCT 
CGCTGGAAAA GTGAAAACGA ACAACTTTTA AATCAGATGG AATCAGAAAA AGAAAGCTTG 
CAGAGTAAAG TTAATCACTT GGAAACTTGT CTTAAGACAC AACAAATAAA AAGTCATGAA 
TATAATGAGA GGGTAAGAAC TCTGGAGATG GAAAGAGAAA ATCTAAACGT GGAGATCAGG 
AACCTTCAAA ATGTGATTGA CAGCAAAACC GCAGAGGCAG AGACACAGAA ACGAGCTTAT 
GGAGAACTGC AACAGAAAGC TGAGTTCTCA GATCAGAAAC ACGAGAAGGA GATAGAAAAT 
ATGTGTTTGA AAATCTCTCA CCTCACTGGG CAAGTGGAAG ACCTAGAACA TAAGCTTCAG 
TTACTGTCAA GTGAAATCAT GGACAAAGAC CAGCGTTACC AAGACTTGCA TGCTGAATCT 
GAGAGCCTCA GGGATCTGCT AAAATCCAGA GATTCCTCTG CGATGACAAT TGAGCCTCAT 
CAGAGAAGTC GTTTAGCTTT TGAACAGCAG TCTGCACTGA ACAATTCCTT TGCAAATATA 
ATCGGAGAGC AAGAAAGTGT GCCTTCAGAA AGGAGCAGAT GCCATGTAGC AACAGACCAA 
AGTCCGAAAA GTTCATCCAT GTTGCAAAAT AGAGTTGTTT CTCTTGAATT TTCCTTAGAG 
TCGCAAAAGC AGATGAACTC AGATCTGCAG AAGCAGTGTG AAGAGTTGGT GCAAATCAAA 
GGAGAAATTG AAGAAAACCT CATGAAAGCA GAACAGATGC ATCAAAGTTT TGTGGCTGAA 
ACAAGCCAAC GCATTAGTAA GTTACAAGAA GACACTTCTG TTCATCAGAA TGTTGTTGCT 
GAAACTTTAG CTGCCCTGGA GAGCAAGGAA AGAGAGTTGC AACTTTTGAA TGAAAAATTA 
GAAACTGAGC AGGCAGAGAC TGGAGAGTTA AAAAAGAACA ACCATTTACT TCAGGAATCT 
CTAAAGGAGC TACAGTTTTT GTCTGAAACC CTGAGCTTGG AGAAAAAGGA ACTGAATTCC 
ATCATTTCTC TAAATAAAAA GGACATTGAA GAGTTGACCC AAGAGAATGG GACTCTCAAG 
GAAATTAATG CCACCTTAAC TCAAGAGAAG ATGAACTTGC TCCAAAAAAC CGAGAGTTTT 
TCGAACTGTA TAGATGAAAG AGACAGAAGC ATTTCAGAGT TATCTAATCA GTACAAACAA 
GAAAGGCTTA CTTTACTACA AAGATGTGAA GAAACAGGAA ATGCATTTCA GGATCTTAGT 
GAAAAATATA AAGCGGTTCA GGAAAAGAAC TCTAAATTAG AATGCTTGCT GAGTGAGTGC 
ACGGGTGTTT GTGAAGACAG AAAAAATGAA TTAGGACAGC TAAAGGAAAC ATTTGCAAGG 
GAACACCAAG CATTTGTATC ACAGTTAGCA TTAGCTGAAG AAAGAAACCA GAATTTAATT 
GTGGAGTTGG AGACAGTGCA GCAAGCCCTG CAGTCTGAGA TTACAGATAT CCAAAACAGC 
TCCAAGAGGG AGACTGATGG TTTAAAGCAA GAAATCATGA ATTTAAAGGA AGAACAAAAT 
AAGATGCAAC AGGAAGTTAG TGCCTTATTA CAAGAGAATG AACACCTGAT GGAATTAATG 
AAGACGAAAC ATGAGCATCA ATGTCTAGAA TTGGAACCAA TTCAAGACTC TGAGAAAGAA 
GAGAGGGAGA TAAATACATG TCACCTTCAA CTTCCGATGG ATCTTGATGT TAAAGATACT 
TCTCCAGACA CTTGTAATGC ACAGCTGGTG CAAGTAGAAT CTAAAGTCAG AAACATGGAA 
TTAAAACTTC AGAAGAGTGA GAAGGAAGAG TGTCTACAGT ATGAGTTACA AACTAGAGAA 
TTAGAAACTG AAGATTTGCA ACAAGATCCT CAGTCACAAG ATATTAGTGG CCTTGGAGAC 
TCTGAAATAG ATCCAGAAGA AAAATATATT TCAGTGCTTC AGGAGTTGTC AACAAGTCAG 
CAGGACAATG CCCACCTGCA GTGTTCTCTA CAGACAGCCA TGAACAAGCT GAATGAGTTA 
GAGAAAATGT GTGAAGCCCT GCAGGTTGAA AAGTCTGAAC TCATATCTGA GCTGAATGAC 
TCAAGATCAG AATGTATCAC AGCAACTAGT AAAATGGCAG AAGAGGTAGG GAAACTAGTA 
AATGAAGTTG AAACATTAAA TGACGAAAAT GCACTTCTCC AAGGCGAGTT AGTGGAAGAA 
ACACCAGAAG GTGAATTTGG TGAACAACAA GATGAGCAGA CATCTGTGAA CTTAAATCCT 
TTGGATGACA GTAATTTCTA TGAGCATTTG ACCATGTCAA GCAAAGAGGT TCAAATGCAC 
TTTGCTGAAT TACAGGAGAA ATTTTCATCT TTACAGAGTG AGCACAAAAT TTTACACGAA 
CAGCACTGTC TGATGAGCTC TAAGATGTCA GAGCTGCAGT CCTACGTCGA CACGTTAAAG 
GCTGAAAATT CAGTCTTGTC GATGAGTCTG AGAAGCTCTC AAGGTGACTT GGTAAAGGAG 
GTGCCACCAG GACCTGGGGA GGAGCATTTA CTGTCCTTGT CATTTTCTTG TGTGACTGAC 
AGCCCTGGTA AGGCAAGTTT GGGAGAGTCC TCTTTTTACA AAGATCTTCT AGAGCACACA 
ACAGAAACGT CCCTTTTGAA TAATCTAGAA GGGACTGTTT CGGCAAACCA GTCCAATGTA 
GAGGAAGTCT CTTGTAGTAG TCTGGAGGAG GAGAATCTGA CCGAGAAAGA GATCCCCTCT 
GTCCCACTGA GGAGCGCTCG AGAACTTGAG ACCCTCTGTC AGACGTACCT CGAGTCCCTC 
AAGCAGCTGG AAGAGGAAAT CGAAAGTCAA GGGATTACGA AAAACAAGGA GATCAAAGAG 
CTCAAGGAGC TACTGAGTTC TTCGAGGGAG GAGCTTGACG ACCTCAGGAA GCAGTATTTG 
TCAGAAAATG AACAGTGGCA GCAGAAGCTG ACAGATGTGA CAGCGGAGAT GGAGTCCAAG 
TTGGCGGCTG AAAAGAGACA CGCAGAGCAC CTTACACTTG AGCTTGAGGT GGCGCGGCTT 
CAGCTGCAGG GTCTGGACTT AAGCTCTCGG TCTCTGCTTG GCACCGACAT AGAAGATGCT 
ATTCTGGGTG GAAACGATAG CTGTGACATA AGAGAATCAG AAGAATATAT TTCAGAAACT 
AAAGAGAGGA CCCCAAAGCA TGAGATTCAC CAGATTTGTG AAAAAGATGT TCAGCAGGAC 
CTCAGTCTAG AGATGGAGAA AATAACAAAG ACTGGTGCAA CCAAACTTAC AGAAGAATGG 
TCCAGAGAGC AGTCCTCAGA AAGCAGTCAT GAGACCCCAG TGGAAGATCC AGCCCAGGGC 
TGTTCAGGAT GCATTTCTGA ACTGTCCCTT TCTGGGCCTA ATGCTTCAGT GCCTAGGGAT 
TTTCTGGAGA ATCAGGTAAC CATTCAGAGT CTCGAGCTGA AAGTAAAAGA GACATCAAAT 
GGGAATTTGA GATTACTTCA TGGCATAGAG GAGCGTGACC AAAAAGTTGC CAACTTGCTA 
AATGAAATCA AAGAGTTAGA CTCAAAATTC CATTTACTGG AAGTACAATT AACTACCAAG 
ATTGAAGCAT GTGTGGCATT GGAAAAAATA GTTGAGGAAC TTAAGAAAGA AAAATTAGAT 
TTAAATGAAA AATTGGAATC CTTTTCTTGT CATAACCAGA GAGAAGAAAG TTCAGGAGGT 
CTCACTTCCA ACTTAGAAAT GGTCACAAGC AAATTTCCAC ATGAAGGTAT TGAAGATGAT 
GTAGCCAAGG TGACTGACAA CTGGAGAGAG AAATGTCTCC AGGTGGAAAA TGAGCTACAG 
AGGATTCAAT CTGAGAAAGA TAGCATGGAG CATCATGCCC TCTCTGCTGA AGCCAGCTTA 
GAGGCAGTTC AAACAGAGAA ACTATATTTA GAAAAAGACA ATGAAAATAA ACAAACAGTT 
ATAACCTGTC TTGAGGAAGG ACTCTCAGTG GTCACAAGTG AGAGAGATCA GCTTCGTGGA 
GAGTTAGATA CTTTGTCAAA AGAAAATCAA GAGCTAGATC AGATGTCTGA AAAGATGAAG 
GAGAAAATAC GGGAGCTTGA ATCTCATCAA AGTGAGTGTC TGCATCTCCA AGAGTGGCTT 
CAGAGTTTGG AAAAGGACTC ACAGGCACTG TCTCTGGTAA GAAGTGAGCT GGAAAACCAA 
ATTGAACAAC TGAATAAAGA GAAAGACTCA CTTGTATGGG AATCCGAAAG CCTGCAGACC 
AAACTGAGCG AATCGGAGCA TGAAAAGCTG GCCATCACCA AGGCTTTGGA GGCTGCCCTG 
ATGGAGAAGG GTGAGGTCGC GGTGAGGCTG AGCTCCACGC AAGAGGAAGT GCACCAGTTG 
AGAAAAGGCA TTGAGAAACT CAGGGTCCGC ATTGAGGCTG ATGAAAAGAA GCAGCTCCAT 
GTCTCAGAGA AACTGAAAGA AAGCGAGCGG AGGAATGACT CGCTTCAGGA CAAGGTTGAG 
ACCCTTGAAA GGGAGTTGCA GATGGCAGAA GAAAACCAAG AGCTGGTGAT TCTTGATGCT 
GAGAATTGCA AAGCAGAGGT GGAAACCCTA AAAACACAAA TAGAATTGAT GACTGAACGC 
CTAAAAGATT TAGAATTAGA CCTTGTCACA ATACGGTCTG AAAAAGAAAA TCTTTTGAAA 
CAGCTACAAG AGAAACAAGG TCAGGCGTCT GAATTAGATA CGCTACTCTC TTCATTGAAA 
AACCTATTAG AAGAAAAGGA GCGAGAGAAA ATACAGACGA AAGAAGAAGC TAAAAGTGCA 
GTGGAGATGC TGCAAACACA GTTGAGAGAG CTAACTGAGG AAATAGCAGC CTTATGTGAT 
GACCAGGAGA CATGGAAGGT CGAAGAACAG AGTCTGGACT CTCCTGCCCA GGAGGTACAA 
CAGCTGAGAA ATAACATTGA AAAGCTGAAA GTCCACTTGG ACATTGATAA AAAGAAGCAG 
CTCCAAATCC TAGAAAAACT GAAGGAAAGT GAGCATCAGG CCGATTTCCT TAAGGATACA 
GTTGAGAATC TTGAAAGAGA ACTCAAGCTA TCAGGAGAAA ACCAAGAGCA TGTGACTCTT 
GAGGCTGAGA AGTCCAAAGC AGAAGTAGAG ACCCTGAAAG CAACAGTGGA GAAGATGGAC 
CAAAATCTGA GAGGTTTGCA ATTAGATCTT GTCAATATAA GATCAGAAAA AGAAGATCTG 
ACTAAAGAAT TACAGAAAGA GCAGAGTCGA GTATCTGAAT TAGAAACGTT AAATTCTTCA 
TTTGAAAACC TATTGCGGGA AAAAGAGCAA GAAAAAGTAC AGATGAAGGA AGAATCTAAA 
GCTGCAGTGG AGATGCTGCA AACACAGCTG AAAGAGCTAA AGCTAAACGA GAAAATGGCA 
GCCTTGGGTA ATGACCAAGA GACCCGGAAG GTCAAAGAAC AGAGCCTGAG TAGTCAAGTA 
GATTCCCTTG AACATGAGAA GGCTCAGTTG CTGCAGGACC TTGATGATGC CAAAAGTAAT 
TACATGATTT TGCAGTCTTC TGTGAATGAC CTCATTCAAG AAGTTGAAGA TGGCAAACAG 
AAACTAGAGA AAAAGGATGA AGAAATCAGT ATACTAAAAA GTCAGACTCG GGACCAAGAG 
CAGCTTGTCT CTAAACTGTC CCAGATGGAA GGAGAACAGC AACTTTGGAA GAAGCAAAAG 
GCAGACCTGG AAAATCTGAT GGTGGAATTG GAGCAGAAGA TTCAGGTGCT GCAATCCAAA 
AACGATGCTT TGCAGGACAC CTTAGAAGCT CTGCAGAATT CTTCCAGGAA TCTGGAGAAA 
GAGCTTGAAT TGACAAAATT GGAAAAAATG TCCTTTGTTG AAAAAGTAAA CACAATGACT 
GTGAAGGAAA CTGAGCTTCA GAAGGAAATA CATGCAGTGA TACAAAAAAC GGTGGCACTG 
AAAGAAGAAT TTAGTGGGGA GAAAAACAGG CTAATGGAAG AATTAAATTT AATGTTGGAA 
GAAGTGAAGA GCAGCAAAGG TCAACTGAAG GAGCTCATGC TAGAAAATAG TGAATTGAAG 
AAGAGTTTGG ATTGTGTACA CAAAGACCGT ATGGAAGAAC AAGGGAAAAT GAGAGGAGAA 
ATAGCTGAAT ATCAGCTACG GCTTCAGGAA GCTGAAAACA AACATCAGGC TTTGCTTCTA 
GATACAAACA AACAGCATGA AATGGAAATC CAGACATACC GAGAGAAACT GACTTCTAAG 
GAGGAATGTC TCAGCTCACA GAAGGTGGAG ATAGACCTCT TAAAGTCCAG TAAAGAAGAA 
CTCAATAATT CTTTGAAAGC GACCACTGAG ATTTTAGAAG AATTGAAGAA AACCAAGATG 
GAAAATCTAA AACATGCAGA TAAATTGAAG AAGGAAAACG ATCGTGCCCA GAGTAAAATA 
AAGTTGTTGG TGAAATCCTG TAAACAGCTG GAAGAGGAAA AGGTGATGTT GCAGAAAGAA 
CTATCTCATC TTGAAGCTGC ACAGGAGAAA CAGAGAGCAG ATACCGTCGT GGATGCTAAT 
GTCGACGAAT TAATAACTGA AATGAAAGAA CTGAAAGAAA CCCTTGAAGA AAAAACCAAG 
GAGGCAGACG AATACCTGGA TAAATACTGT TCCCTGCTCA TAAGCCATGA GAAGTTAGAG 
AAAGCCAAGG AGATGTTAGA AACACAAGTT GCTCGTCTGA GTTCACAACA ATCTAAACTG 
AATCTTCGGA GATCTCCTTT GGTGAATTCG CTTGCTCCAG GACCATCCCC AGTCCCTTCT 
GCCACTGAGA AGAAGTTACC ATCCGGCCAA AATAAATCTT CAGGCAAAAG GCAAAGGTCC 
AGTGGGATTC GGGAGGATGG CGGAGAAACC ACGCCTTCTA CACCAGAGAC ATTTTCTAAA 
AAAAGCAGGA AAGCCATCAG AAGTGGTACT CACCTTGCAG AGGATGCAGA AGATGCTGAA 
TTTGAGCCAG AGGGGCTTCC AGAAGTTGTA AAGAAAGGGT TTGCCGACAT CCCAACCGGA 
AAGACTAGCC CCTACATCCT GCGGAGGACA ACCATGGCAA CCAGGACCAG CCCCCGCCTT 
GCTGCCCAGA AGTTAGCAGC ATCCCCGCCA AGTCTCGACA AAGAAAATCT CACCGAGACC 
TCCAAACCAA CAGCTGGTGG CAGCAGATCA CAAAAGGTCA AGGTTTCTCA ACAGAGCCCA 
GTGGATTCAG GCACTGCCTT CCGAGATCCC ATTGGCAGAT CTCTCTCCAT CAGTAATCTT 
CCCGAGAGAA GTCCAGTGGG CAGCCCCAGG GAGGGCCTGA GAGCCAAGCG AGGCCGCCAC 
GCCCCCAGCC CAGAATCCAG CCTGGAGTCC AAGGGCAGCG AGAACTGTAG GGTCCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CENPF ( XM_024976312.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832080.1 Bos taurus centromere protein F (CENPF), transcript variant X2, mRNA.

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CloneID	OBa223624
Clone ID Related Accession (Same CDS sequence)	XM_024976312.1
Accession Version	XM_024976312.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	centromere protein F isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAATAT GTGAAAATCT GGAGAAGACT AAGCAGAAGA TTTCTCATGA ACTTCAAGTC 
AAGGAGTCAC AAGTGAATTT CCAAGAAGGT CAACTGAACT CAAGCAAAAA ACAAATAGAA 
AAGCTGGAAC AGGAACTTAA AAGGTGTAAA TCTGAACTTG AAAGAAGCCA ACAAGCTGCA 
CAGTCTGCGG ATGTCTCTCT GAATTCATGC CTTACACCAC AAAAAATCTT TGCAACTCCG 
CTCACACCAA GCCAGTATTA TAGCGGTTCC AAATATGAAG ATCTAAAAGA AAAATATAAT 
AAAGAAGTTG AAGAACGAAA AAGATTAGAG GCAGAGGTAA AAGCCTTGCA GGCAAAGAAA 
GCAAGCCAGA CTATTCCCCA AAGCACCATG AATCACCGGG ACATCGCCCG ACATCAGGCT 
TCTTCATCTG TGTTCTCATG GCAACAAGAA AAAACCCCAA GCCGTCTTTC ATCTAACACT 
CTGAAAACTC CAGTTAGGAG AGATTTCTCT GCATCTCATT TTTCTGGGGA ACAAGAAGTG 
ACTCCGAGTA GATCAACTTT GCAAATAGGG AAAACAGATG CTAATAGCAG TTTCTGTGAT 
AATTCTAGCA ATTCTCATCT TTTGGACCAG TTAAAAGCAC AGAACCAAGA ACTAAGGAGC 
AAGATGAGTG AGCTGGAACT ACGTCTGCAA GGACAAGAAA AAGAAATGAA AGGCCAAGTG 
AATAAACTTC AAGAACTCCA ACTGCAACTG GAGAAAGCAA AAGTGGAATT AAATGAAAAA 
GAGAAAGTTT TGAACAAAAA CCGGGATGAA CTAGTGAGAA CAACTTCACA GTATGACCAG 
GCATCAGCCA AGTGTACTGC TTTGGAACAA AAATTGAAAA AATTGACTGA AGATTTGAGT 
TGTCAGCGTC AAAATGCAGA AAGTGCCAGA TGTTCTCTGG AACAGAAAAT CAAGGAGAAA 
GAAAAAGAGT TCCAAGAGGA GCTCTCCCGT CAACAGCGTT CCTTCCAAAC CCTGGACCAG 
GAGTGCACTC AGATGAAAGC CAGGCTCACC CAGGAGCTAC AGCAAGCTAA GAACACATAC 
AACATCCTAC AGGCTGAACT GGATAAAGTC ACATCTGTAA AGCAACAGAT AGAAAAAAAA 
TTGGAAGAGT TTAAGCAAAA TTTCAGCAAA ACTGAACAGG CCTTACAGGC AAGTCAGACC 
AAGGAAAATG AGCTGAGGAG AAGCAGTGAG GAAATGAAGA GGGAAAACAG CCTCCTCAAG 
AGTCAGTCTG AGCAAAGGGC CCGAGAAGTC TGCCACCTGG AGGAAGAACT GAAGAAGGCC 
AAGCAGTGTT TGAGTCAGAG CCAGAATTTT GCAGAAGAAA TGAAGGCTAA GAATACCTCT 
CAGGAAACCA TGTTAAGAGA TCTTCAAGAA AAGATAAATC AGCAAGAGAA TTCCTTGACT 
TTAGAGAAAC TGAAGCTTGC CTTGGCTGAC CTGGAAAAGC AGCGAGATTG TTCTCAAGAC 
CTTTTGAAGA AAAGGGAACA TCACATTGAA CAACTGAACG AAAAGTTAAG CAAAACAGAG 
AGAGAATCTG AAGCATTGTT GAGTGCTTTG GAATTAAAGA AGAAAGAATA TGAAGAATTA 
AAAGAAGAGA AAACTCTGTT TTCTCGCTGG AAAAGTGAAA ACGAACAACT TTTAAATCAG 
ATGGAATCAG AAAAAGAAAG CTTGCAGAGT AAAGTTAATC ACTTGGAAAC TTGTCTTAAG 
ACACAACAAA TAAAAAGTCA TGAATATAAT GAGAGGGTAA GAACTCTGGA GATGGAAAGA 
GAAAATCTAA ACGTGGAGAT CAGGAACCTT CAAAATGTGA TTGACAGCAA AACCGCAGAG 
GCAGAGACAC AGAAACGAGC TTATGGAGAA CTGCAACAGA AAGCTGAGTT CTCAGATCAG 
AAACACGAGA AGGAGATAGA AAATATGTGT TTGAAAATCT CTCACCTCAC TGGGCAAGTG 
GAAGACCTAG AACATAAGCT TCAGTTACTG TCAAGTGAAA TCATGGACAA AGACCAGCGT 
TACCAAGACT TGCATGCTGA ATCTGAGAGC CTCAGGGATC TGCTAAAATC CAGAGATTCC 
TCTGCGATGA CAATTGAGCC TCATCAGAGA AGTCGTTTAG CTTTTGAACA GCAGTCTGCA 
CTGAACAATT CCTTTGCAAA TATAATCGGA GAGCAAGAAA GTGTGCCTTC AGAAAGGAGC 
AGATGCCATG TAGCAACAGA CCAAAGTCCG AAAAGTTCAT CCATGTTGCA AAATAGAGTT 
GTTTCTCTTG AATTTTCCTT AGAGTCGCAA AAGCAGATGA ACTCAGATCT GCAGAAGCAG 
TGTGAAGAGT TGGTGCAAAT CAAAGGAGAA ATTGAAGAAA ACCTCATGAA AGCAGAACAG 
ATGCATCAAA GTTTTGTGGC TGAAACAAGC CAACGCATTA GTAAGTTACA AGAAGACACT 
TCTGTTCATC AGAATGTTGT TGCTGAAACT TTAGCTGCCC TGGAGAGCAA GGAAAGAGAG 
TTGCAACTTT TGAATGAAAA ATTAGAAACT GAGCAGGCAG AGACTGGAGA GTTAAAAAAG 
AACAACCATT TACTTCAGGA ATCTCTAAAG GAGCTACAGT TTTTGTCTGA AACCCTGAGC 
TTGGAGAAAA AGGAACTGAA TTCCATCATT TCTCTAAATA AAAAGGACAT TGAAGAGTTG 
ACCCAAGAGA ATGGGACTCT CAAGGAAATT AATGCCACCT TAACTCAAGA GAAGATGAAC 
TTGCTCCAAA AAACCGAGAG TTTTTCGAAC TGTATAGATG AAAGAGACAG AAGCATTTCA 
GAGTTATCTA ATCAGTACAA ACAAGAAAGG CTTACTTTAC TACAAAGATG TGAAGAAACA 
GGAAATGCAT TTCAGGATCT TAGTGAAAAA TATAAAGCGG TTCAGGAAAA GAACTCTAAA 
TTAGAATGCT TGCTGAGTGA GTGCACGGGT GTTTGTGAAG ACAGAAAAAA TGAATTAGGA 
CAGCTAAAGG AAACATTTGC AAGGGAACAC CAAGCATTTG TATCACAGTT AGCATTAGCT 
GAAGAAAGAA ACCAGAATTT AATTGTGGAG TTGGAGACAG TGCAGCAAGC CCTGCAGTCT 
GAGATTACAG ATATCCAAAA CAGCTCCAAG AGGGAGACTG ATGGTTTAAA GCAAGAAATC 
ATGAATTTAA AGGAAGAACA AAATAAGATG CAACAGGAAG TTAGTGCCTT ATTACAAGAG 
AATGAACACC TGATGGAATT AATGAAGACG AAACATGAGC ATCAATGTCT AGAATTGGAA 
CCAATTCAAG ACTCTGAGAA AGAAGAGAGG GAGATAAATA CATGTCACCT TCAACTTCCG 
ATGGATCTTG ATGTTAAAGA TACTTCTCCA GACACTTGTA ATGCACAGCT GGTGCAAGTA 
GAATCTAAAG TCAGAAACAT GGAATTAAAA CTTCAGAAGA GTGAGAAGGA AGAGTGTCTA 
CAGTATGAGT TACAAACTAG AGAATTAGAA ACTGAAGATT TGCAACAAGA TCCTCAGTCA 
CAAGATATTA GTGGCCTTGG AGACTCTGAA ATAGATCCAG AAGAAAAATA TATTTCAGTG 
CTTCAGGAGT TGTCAACAAG TCAGCAGGAC AATGCCCACC TGCAGTGTTC TCTACAGACA 
GCCATGAACA AGCTGAATGA GTTAGAGAAA ATGTGTGAAG CCCTGCAGGT TGAAAAGTCT 
GAACTCATAT CTGAGCTGAA TGACTCAAGA TCAGAATGTA TCACAGCAAC TAGTAAAATG 
GCAGAAGAGG TAGGGAAACT AGTAAATGAA GTTGAAACAT TAAATGACGA AAATGCACTT 
CTCCAAGGCG AGTTAGTGGA AGAAACACCA GAAGGTGAAT TTGGTGAACA ACAAGATGAG 
CAGACATCTG TGAACTTAAA TCCTTTGGAT GACAGTAATT TCTATGAGCA TTTGACCATG 
TCAAGCAAAG AGGTTCAAAT GCACTTTGCT GAATTACAGG AGAAATTTTC ATCTTTACAG 
AGTGAGCACA AAATTTTACA CGAACAGCAC TGTCTGATGA GCTCTAAGAT GTCAGAGCTG 
CAGTCCTACG TCGACACGTT AAAGGCTGAA AATTCAGTCT TGTCGATGAG TCTGAGAAGC 
TCTCAAGGTG ACTTGGTAAA GGAGGTGCCA CCAGGACCTG GGGAGGAGCA TTTACTGTCC 
TTGTCATTTT CTTGTGTGAC TGACAGCCCT GGTAAGGCAA GTTTGGGAGA GTCCTCTTTT 
TACAAAGATC TTCTAGAGCA CACAACAGAA ACGTCCCTTT TGAATAATCT AGAAGGGACT 
GTTTCGGCAA ACCAGTCCAA TGTAGAGGAA GTCTCTTGTA GTAGTCTGGA GGAGGAGAAT 
CTGACCGAGA AAGAGATCCC CTCTGTCCCA CTGAGGAGCG CTCGAGAACT TGAGACCCTC 
TGTCAGACGT ACCTCGAGTC CCTCAAGCAG CTGGAAGAGG AAATCGAAAG TCAAGGGATT 
ACGAAAAACA AGGAGATCAA AGAGCTCAAG GAGCTACTGA GTTCTTCGAG GGAGGAGCTT 
GACGACCTCA GGAAGCAGTA TTTGTCAGAA AATGAACAGT GGCAGCAGAA GCTGACAGAT 
GTGACAGCGG AGATGGAGTC CAAGTTGGCG GCTGAAAAGA GACACGCAGA GCACCTTACA 
CTTGAGCTTG AGGTGGCGCG GCTTCAGCTG CAGGGTCTGG ACTTAAGCTC TCGGTCTCTG 
CTTGGCACCG ACATAGAAGA TGCTATTCTG GGTGGAAACG ATAGCTGTGA CATAAGAGAA 
TCAGAAGAAT ATATTTCAGA AACTAAAGAG AGGACCCCAA AGCATGAGAT TCACCAGATT 
TGTGAAAAAG ATGTTCAGCA GGACCTCAGT CTAGAGATGG AGAAAATAAC AAAGACTGGT 
GCAACCAAAC TTACAGAAGA ATGGTCCAGA GAGCAGTCCT CAGAAAGCAG TCATGAGACC 
CCAGTGGAAG ATCCAGCCCA GGGCTGTTCA GGATGCATTT CTGAACTGTC CCTTTCTGGG 
CCTAATGCTT CAGTGCCTAG GGATTTTCTG GAGAATCAGG TAACCATTCA GAGTCTCGAG 
CTGAAAGTAA AAGAGACATC AAATGAGAAT TTGAGATTAC TTCATGGCAT AGAGGAGCGT 
GACCAAAAAG TTGAAAACTT GCTAAATGAA ATCAAAGAGT TAGACTCAAA ATTCCATTTA 
CTGGAAGTAC AATTAACTAC CAAGATTGAA GCATGTGTGG CATTGGAAAA AATAGTTGAG 
GAACTTAAGA AAGAAAAATT AGATTTAAAT GAAAAATTGG AATCCTTTTC TTGTCATAAC 
CAGAGAGAAG AAAGTTCAGG AGGTCTCACT TCCAACTTAG AAATGGTCAC AAGCAAATTT 
CCACATGAAG GTATTGAAGA TGATGTAGCC AAGGTGACTG ACAACTGGAG AGAGAAATGT 
CTCCAGGTGG AAAATGAGCT ACAGAGGATT CAATCTGAGA AAGATAGCAT GGAGCATCAT 
GCCCTCTCTG CTGAAGCCAG CTTAGAGGCA GTTCAAACAG AGAAACTATA TTTAGAAAAA 
GACAATGAAA ATAAACAAAC AGTTATAACC TGTCTTGAGG AAGGACTCTC AGTGGTCACA 
AGTGAGAGAG ATCAGCTTCG TGGAGAGTTA GATACTTTGT CAAAAGAAAA TCAAGAGCTA 
GATCAGATGT CTGAAAAGAT GAAGGAGAAA ATACGGGAGC TTGAATCTCA TCAAAGTGAG 
TGTCTGCATC TCCAAGAGTG GCTTCAGAGT TTGGAAAAGG ACTCACAGGC ACTGTCTCTG 
GTAAGAAGTG AGCTGGAAAA CCAAATTGAA CAACTGAATA AAGAGAAAGA CTCACTTGTA 
TGGGAATCCG AAAGCCTGCA GACCAAACTG AGCGAATCGG AGCATGAAAA GCTGGCCATC 
ACCAAGGCTT TGGAGGCTGC CCTGATGGAG AAGGGTGAGG TCGCGGTGAG GCTGAGCTCC 
ACGCAAGAGG AAGTGCACCA GTTGAGAAAA GGCATTGAGA AACTCAGGGT CCGCATTGAG 
GCTGATGAAA AGAAGCAGCT CCATGTCTCA GAGAAACTGA AAGAAAGCGA GCGGAGGAAT 
GACTCGCTTC AGGACAAGGT TGAGACCCTT GAAAGGGAGT TGCAGATGGC AGAAGAAAAC 
CAAGAGCTGG TGATTCTTGA TGCTGAGAAT TGCAAAGCAG AGGTGGAAAC CCTAAAAACA 
CAAATAGAAT TGATGACTGA ACGCCTAAAA GATTTAGAAT TAGACCTTGT CACAATACGG 
TCTGAAAAAG AAAATCTTTT GAAACAGCTA CAAGAGAAAC AAGGTCAGGC GTCTGAATTA 
GATACGCTAC TCTCTTCATT GAAAAACCTA TTAGAAGAAA AGGAGCGAGA GAAAATACAG 
ACGAAAGAAG AAGCTAAAAG TGCAGTGGAG ATGCTGCAAA CACAGTTGAG AGAGCTAACT 
GAGGAAATAG CAGCCTTATG TGATGACCAG GAGACATGGA AGGTCGAAGA ACAGAGTCTG 
GACTCTCCTG CCCAGGAGGT ACAACAGCTG AGAAATAACA TTGAAAAGCT GAAAGTCCAC 
TTGGACATTG ATAAAAAGAA GCAGCTCCAA ATCCTAGAAA AACTGAAGGA AAGTGAGCAT 
CAGGCCGATT TCCTTAAGGA TACAGTTGAG AATCTTGAAA GAGAACTCAA GCTATCAGGA 
GAAAACCAAG AGCATGTGAC TCTTGAGGCT GAGAAGTCCA AAGCAGAAGT AGAGACCCTG 
AAAGCAACAG TGGAGAAGAT GGACCAAAAT CTGAGAGGTT TGCAATTAGA TCTTGTCAAT 
ATAAGATCAG AAAAAGAAGA TCTGACTAAA GAATTACAGA AAGAGCAGAG TCGAGTATCT 
GAATTAGAAA CGTTAAATTC TTCATTTGAA AACCTATTGC GGGAAAAAGA GCAAGAAAAA 
GTACAGATGA AGGAAGAATC TAAAGCTGCA GTGGAGATGC TGCAAACACA GCTGAAAGAG 
CTAAGTGAAG AAGTAGCAAT CTTGTGTGTT GACCAGGAGA CCTGGAAGGT TGAAGAACAG 
AGTCTGGACT CCCCACTTGA GGAGATACAA CAGCTGAGAA ATAACATTGG AAAGCTGAAA 
GTCTGCCTAG ATGCTGATAA AAAGCAGCAG CTCCATATCT TAGAAAAACT GAAGGAAAGT 
GAGCATCAGG CCGATTTCCT TAAGGATACA GTTGAGAACC TTGAAAGAGA ACTCAAGCTA 
TCAGGAGAAA ACCAAGAGCA TGTGACTCTT GAGGCTGAGA AGTCCAAAGC AGAAGTAGAG 
ACCCTGAAAG CAACAGTGGA GGAGATGGAC CAAAATCTGA GAGGTTTGCA ATTAGATCTT 
GTCAATATAA GATCAGACAA AGAAGATCTG ACTAAAGAAT TACAGAAAGA GCAGAGTCGA 
GTGTCTGAAT TAGAAACGTT AAATTCTTCA TTTGAAAACC TATTGCGGGA AAAAGAGCAA 
GAAAAAGTAC AGATGAAGGA AGAATCTAAA GCTGCAGTGG AGATGCTGCA AAAAGAATTA 
GAAGAGCTAA ACGAGAAAAT GGCAGCCTTG GGTAATGACC AAGAGACCCG GAAGGTCAAA 
GAACAGAGCC TGAGTAGTCA AGTAGATTCC CTTGAACATG AGAAGGCTCA GTTGCTGCAG 
GACCTTGATG ATGCCAAAAG TAATTACATG ATTTTGCAGT CTTCTGTGAA TGACCTCATT 
CAAGAAGTTG AAGATGGCAA ACAGAAACTA GAGAAAAAGG ATGAAGAAAT CAGTATACTA 
AAAAGTCAGA CTCGGGACCA AGAGCAGCTT GTCTCTAAAC TGTCCCAGAT GGAAGGAGAA 
CAGCAACTTT GGAAGAAGCA AAAGGCAGAC CTGGAAAATC TGATGGTGGA ATTGGAGCAG 
AAGATTCAGG TGCTGCAATC CAAAAACGAT GCTTTGCAGG ACACCTTAGA AGCTCTGCAG 
AATTCTTCCA GGAATCTGGA GAAAGAGCTT GAATTGACAA AATTGGAAAA AATGTCCTTT 
GTTGAAAAAG TAAACACAAT GACTGTGAAG GAAACTGAGC TTCAGAAGGA AATACATGCA 
GTGATACAAA AAACGGTGGC ACTGAAAGAA GAATTTAGTG GGGAGAAAAA CAGGCTAATG 
GAAGAATTAA ATTTAATGTT GGAAGAAGTG AAGAGCAGCA AAGGTCAACT GAAGGAGCTC 
ATGCTAGAAA ATAGTGAATT GAAGAAGAGT TTGGATTGTG TACACAAAGA CCGTATGGAA 
GAACAAGGGA AAATGAGAGG AGAAATAGCT GAATATCAGC TACGGCTTCA GGAAGCTGAA 
AACAAACATC AGGCTTTGCT TCTAGATACA AACAAACAGC ATGAAATGGA AATCCAGACA 
TACCGAGAGA AACTGACTTC TAAGGAGGAA TGTCTCAGCT CACAGAAGGT GGAGATAGAC 
CTCTTAAAGT CCAGTAAAGA AGAACTCAAT AATTCTTTGA AAGCGACCAC TGAGATTTTA 
GAAGAATTGA AGAAAACCAA GATGGAAAAT CTAAAACATG CAGATAAATT GAAGAAGGAA 
AACGATCGTG CCCAGAGTAA AATAAAGTTG TTGGTGAAAT CCTGTAAACA GCTGGAAGAG 
GAAAAGGTGA TGTTGCAGAA AGAACTATCT CATCTTGAAG CTGCACAGGA GAAACAGAGA 
GCAGATACCG TCGTGGATGC TAATGTCGAC GAATTAATAA CTGAAATGAA AGAACTGAAA 
GAAACCCTTG AAGAAAAAAC CAAGGAGGCA GACGAATACC TGGATAAATA CTGTTCCCTG 
CTCATAAGCC ATGAGAAGTT AGAGAAAGCC AAGGAGATGT TAGAAACACA AGTTGCTCGT 
CTGAGTTCAC AACAATCTAA ACTGAATCTT CGGAGATCTC CTTTGGTGAA TTCGCTTGCT 
CCAGGACCAT CCCCAGTCCC TTCTGCCACT GAGAAGAAGT TACCATCCGG CCAAAATAAA 
TCTTCAGGCA AAAGGCAAAG GTCCAGTGGG ATTCGGGAGG ATGGCGGAGA AACCACGCCT 
TCTACACCAG AGACATTTTC TAAAAAAAGC AGGAAAGCCA TCAGAAGTGG TACTCACCTT 
GCAGAGGATG CAGAAGATGC TGAATTTGAG CCAGAGGGGC TTCCAGAAGT TGTAAAGAAA 
GGGTTTGCCG ACATCCCAAC CGGAAAGACT AGCCCCTACA TCCTGCGGAG GACAACCATG 
GCAACCAGGA CCAGCCCCCG CCTTGCTGCC CAGAAGTTAG CAGCATCCCC GCCAAGTCTC 
GACAAAGAAA ATCTCACCGA GACCTCCAAA CCAACAGCTG GTGGCAGCAG ATCACAAAAG 
GTCAAGGTTT CTCAACAGAG CCCAGTGGAT TCAGGCACTG CCTTCCGAGA TCCCATTGGC 
AGATCTCTCT CCATCAGTAA TCTTCCCGAG AGAAGTCCAG TGGGCAGCCC CAGGGAGGGC 
CTGAGAGCCA AGCGAGGCCG CCACGCCCCC AGCCCAGAAT CCAGCCTGGA GTCCAAGGGC 
AGCGAGAACT GTAGGGTCCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832080.1
CDS	198..9761
Translation	MEICENLEKTKQKISHELQVKESQVNFQEGQLNSSKKQIEKLEQELKRCKSELERSQQAAQSADVSLNSCLTPQKIFATPLTPSQYYSGSKYEDLKEKYNKEVEERKRLEAEVKALQAKKASQTIPQSTMNHRDIARHQASSSVFSWQQEKTPSRLSSNTLKTPVRRDFSASHFSGEQEVTPSRSTLQIGKTDANSSFCDNSSNSHLLDQLKAQNQELRSKMSELELRLQGQEKEMKGQVNKLQELQLQLEKAKVELNEKEKVLNKNRDELVRTTSQYDQASAKCTALEQKLKKLTEDLSCQRQNAESARCSLEQKIKEKEKEFQEELSRQQRSFQTLDQECTQMKARLTQELQQAKNTYNILQAELDKVTSVKQQIEKKLEEFKQNFSKTEQALQASQTKENELRRSSEEMKRENSLLKSQSEQRAREVCHLEEELKKAKQCLSQSQNFAEEMKAKNTSQETMLRDLQEKINQQENSLTLEKLKLALADLEKQRDCSQDLLKKREHHIEQLNEKLSKTERESEALLSALELKKKEYEELKEEKTLFSRWKSENEQLLNQMESEKESLQSKVNHLETCLKTQQIKSHEYNERVRTLEMERENLNVEIRNLQNVIDSKTAEAETQKRAYGELQQKAEFSDQKHEKEIENMCLKISHLTGQVEDLEHKLQLLSSEIMDKDQRYQDLHAESESLRDLLKSRDSSAMTIEPHQRSRLAFEQQSALNNSFANIIGEQESVPSERSRCHVATDQSPKSSSMLQNRVVSLEFSLESQKQMNSDLQKQCEELVQIKGEIEENLMKAEQMHQSFVAETSQRISKLQEDTSVHQNVVAETLAALESKERELQLLNEKLETEQAETGELKKNNHLLQESLKELQFLSETLSLEKKELNSIISLNKKDIEELTQENGTLKEINATLTQEKMNLLQKTESFSNCIDERDRSISELSNQYKQERLTLLQRCEETGNAFQDLSEKYKAVQEKNSKLECLLSECTGVCEDRKNELGQLKETFAREHQAFVSQLALAEERNQNLIVELETVQQALQSEITDIQNSSKRETDGLKQEIMNLKEEQNKMQQEVSALLQENEHLMELMKTKHEHQCLELEPIQDSEKEEREINTCHLQLPMDLDVKDTSPDTCNAQLVQVESKVRNMELKLQKSEKEECLQYELQTRELETEDLQQDPQSQDISGLGDSEIDPEEKYISVLQELSTSQQDNAHLQCSLQTAMNKLNELEKMCEALQVEKSELISELNDSRSECITATSKMAEEVGKLVNEVETLNDENALLQGELVEETPEGEFGEQQDEQTSVNLNPLDDSNFYEHLTMSSKEVQMHFAELQEKFSSLQSEHKILHEQHCLMSSKMSELQSYVDTLKAENSVLSMSLRSSQGDLVKEVPPGPGEEHLLSLSFSCVTDSPGKASLGESSFYKDLLEHTTETSLLNNLEGTVSANQSNVEEVSCSSLEEENLTEKEIPSVPLRSARELETLCQTYLESLKQLEEEIESQGITKNKEIKELKELLSSSREELDDLRKQYLSENEQWQQKLTDVTAEMESKLAAEKRHAEHLTLELEVARLQLQGLDLSSRSLLGTDIEDAILGGNDSCDIRESEEYISETKERTPKHEIHQICEKDVQQDLSLEMEKITKTGATKLTEEWSREQSSESSHETPVEDPAQGCSGCISELSLSGPNASVPRDFLENQVTIQSLELKVKETSNENLRLLHGIEERDQKVENLLNEIKELDSKFHLLEVQLTTKIEACVALEKIVEELKKEKLDLNEKLESFSCHNQREESSGGLTSNLEMVTSKFPHEGIEDDVAKVTDNWREKCLQVENELQRIQSEKDSMEHHALSAEASLEAVQTEKLYLEKDNENKQTVITCLEEGLSVVTSERDQLRGELDTLSKENQELDQMSEKMKEKIRELESHQSECLHLQEWLQSLEKDSQALSLVRSELENQIEQLNKEKDSLVWESESLQTKLSESEHEKLAITKALEAALMEKGEVAVRLSSTQEEVHQLRKGIEKLRVRIEADEKKQLHVSEKLKESERRNDSLQDKVETLERELQMAEENQELVILDAENCKAEVETLKTQIELMTERLKDLELDLVTIRSEKENLLKQLQEKQGQASELDTLLSSLKNLLEEKEREKIQTKEEAKSAVEMLQTQLRELTEEIAALCDDQETWKVEEQSLDSPAQEVQQLRNNIEKLKVHLDIDKKKQLQILEKLKESEHQADFLKDTVENLERELKLSGENQEHVTLEAEKSKAEVETLKATVEKMDQNLRGLQLDLVNIRSEKEDLTKELQKEQSRVSELETLNSSFENLLREKEQEKVQMKEESKAAVEMLQTQLKELSEEVAILCVDQETWKVEEQSLDSPLEEIQQLRNNIGKLKVCLDADKKQQLHILEKLKESEHQADFLKDTVENLERELKLSGENQEHVTLEAEKSKAEVETLKATVEEMDQNLRGLQLDLVNIRSDKEDLTKELQKEQSRVSELETLNSSFENLLREKEQEKVQMKEESKAAVEMLQKELEELNEKMAALGNDQETRKVKEQSLSSQVDSLEHEKAQLLQDLDDAKSNYMILQSSVNDLIQEVEDGKQKLEKKDEEISILKSQTRDQEQLVSKLSQMEGEQQLWKKQKADLENLMVELEQKIQVLQSKNDALQDTLEALQNSSRNLEKELELTKLEKMSFVEKVNTMTVKETELQKEIHAVIQKTVALKEEFSGEKNRLMEELNLMLEEVKSSKGQLKELMLENSELKKSLDCVHKDRMEEQGKMRGEIAEYQLRLQEAENKHQALLLDTNKQHEMEIQTYREKLTSKEECLSSQKVEIDLLKSSKEELNNSLKATTEILEELKKTKMENLKHADKLKKENDRAQSKIKLLVKSCKQLEEEKVMLQKELSHLEAAQEKQRADTVVDANVDELITEMKELKETLEEKTKEADEYLDKYCSLLISHEKLEKAKEMLETQVARLSSQQSKLNLRRSPLVNSLAPGPSPVPSATEKKLPSGQNKSSGKRQRSSGIREDGGETTPSTPETFSKKSRKAIRSGTHLAEDAEDAEFEPEGLPEVVKKGFADIPTGKTSPYILRRTTMATRTSPRLAAQKLAASPPSLDKENLTETSKPTAGGSRSQKVKVSQQSPVDSGTAFRDPIGRSLSISNLPERSPVGSPREGLRAKRGRHAPSPESSLESKGSENCRVQ

Target ORF information:

RefSeq Version	XM_024976312.1
Organism	Bos taurus(cattle)
Definition	Bos taurus centromere protein F (CENPF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976312.1

ORF Insert Sequence:

ATGGAAATAT GTGAAAATCT GGAGAAGACT AAGCAGAAGA TTTCTCATGA ACTTCAAGTC 
AAGGAGTCAC AAGTGAATTT CCAAGAAGGT CAACTGAACT CAAGCAAAAA ACAAATAGAA 
AAGCTGGAAC AGGAACTTAA AAGGTGTAAA TCTGAACTTG AAAGAAGCCA ACAAGCTGCA 
CAGTCTGCGG ATGTCTCTCT GAATTCATGC CTTACACCAC AAAAAATCTT TGCAACTCCG 
CTCACACCAA GCCAGTATTA TAGCGGTTCC AAATATGAAG ATCTAAAAGA AAAATATAAT 
AAAGAAGTTG AAGAACGAAA AAGATTAGAG GCAGAGGTAA AAGCCTTGCA GGCAAAGAAA 
GCAAGCCAGA CTATTCCCCA AAGCACCATG AATCACCGGG ACATCGCCCG ACATCAGGCT 
TCTTCATCTG TGTTCTCATG GCAACAAGAA AAAACCCCAA GCCGTCTTTC ATCTAACACT 
CTGAAAACTC CAGTTAGGAG AGATTTCTCT GCATCTCATT TTTCTGGGGA ACAAGAAGTG 
ACTCCGAGTA GATCAACTTT GCAAATAGGG AAAACAGATG CTAATAGCAG TTTCTGTGAT 
AATTCTAGCA ATTCTCATCT TTTGGACCAG TTAAAAGCAC AGAACCAAGA ACTAAGGAGC 
AAGATGAGTG AGCTGGAACT ACGTCTGCAA GGACAAGAAA AAGAAATGAA AGGCCAAGTG 
AATAAACTTC AAGAACTCCA ACTGCAACTG GAGAAAGCAA AAGTGGAATT AAATGAAAAA 
GAGAAAGTTT TGAACAAAAA CCGGGATGAA CTAGTGAGAA CAACTTCACA GTATGACCAG 
GCATCAGCCA AGTGTACTGC TTTGGAACAA AAATTGAAAA AATTGACTGA AGATTTGAGT 
TGTCAGCGTC AAAATGCAGA AAGTGCCAGA TGTTCTCTGG AACAGAAAAT CAAGGAGAAA 
GAAAAAGAGT TCCAAGAGGA GCTCTCCCGT CAACAGCGTT CCTTCCAAAC CCTGGACCAG 
GAGTGCACTC AGATGAAAGC CAGGCTCACC CAGGAGCTAC AGCAAGCTAA GAACACATAC 
AACATCCTAC AGGCTGAACT GGATAAAGTC ACATCTGTAA AGCAACAGAT AGAAAAAAAA 
TTGGAAGAGT TTAAGCAAAA TTTCAGCAAA ACTGAACAGG CCTTACAGGC AAGTCAGACC 
AAGGAAAATG AGCTGAGGAG AAGCAGTGAG GAAATGAAGA GGGAAAACAG CCTCCTCAAG 
AGTCAGTCTG AGCAAAGGGC CCGAGAAGTC TGCCACCTGG AGGAAGAACT GAAGAAGGCC 
AAGCAGTGTT TGAGTCAGAG CCAGAATTTT GCAGAAGAAA TGAAGGCTAA GAATACCTCT 
CAGGAAACCA TGTTAAGAGA TCTTCAAGAA AAGATAAATC AGCAAGAGAA TTCCTTGACT 
TTAGAGAAAC TGAAGCTTGC CTTGGCTGAC CTGGAAAAGC AGCGAGATTG TTCTCAAGAC 
CTTTTGAAGA AAAGGGAACA TCACATTGAA CAACTGAACG AAAAGTTAAG CAAAACAGAG 
AGAGAATCTG AAGCATTGTT GAGTGCTTTG GAATTAAAGA AGAAAGAATA TGAAGAATTA 
AAAGAAGAGA AAACTCTGTT TTCTCGCTGG AAAAGTGAAA ACGAACAACT TTTAAATCAG 
ATGGAATCAG AAAAAGAAAG CTTGCAGAGT AAAGTTAATC ACTTGGAAAC TTGTCTTAAG 
ACACAACAAA TAAAAAGTCA TGAATATAAT GAGAGGGTAA GAACTCTGGA GATGGAAAGA 
GAAAATCTAA ACGTGGAGAT CAGGAACCTT CAAAATGTGA TTGACAGCAA AACCGCAGAG 
GCAGAGACAC AGAAACGAGC TTATGGAGAA CTGCAACAGA AAGCTGAGTT CTCAGATCAG 
AAACACGAGA AGGAGATAGA AAATATGTGT TTGAAAATCT CTCACCTCAC TGGGCAAGTG 
GAAGACCTAG AACATAAGCT TCAGTTACTG TCAAGTGAAA TCATGGACAA AGACCAGCGT 
TACCAAGACT TGCATGCTGA ATCTGAGAGC CTCAGGGATC TGCTAAAATC CAGAGATTCC 
TCTGCGATGA CAATTGAGCC TCATCAGAGA AGTCGTTTAG CTTTTGAACA GCAGTCTGCA 
CTGAACAATT CCTTTGCAAA TATAATCGGA GAGCAAGAAA GTGTGCCTTC AGAAAGGAGC 
AGATGCCATG TAGCAACAGA CCAAAGTCCG AAAAGTTCAT CCATGTTGCA AAATAGAGTT 
GTTTCTCTTG AATTTTCCTT AGAGTCGCAA AAGCAGATGA ACTCAGATCT GCAGAAGCAG 
TGTGAAGAGT TGGTGCAAAT CAAAGGAGAA ATTGAAGAAA ACCTCATGAA AGCAGAACAG 
ATGCATCAAA GTTTTGTGGC TGAAACAAGC CAACGCATTA GTAAGTTACA AGAAGACACT 
TCTGTTCATC AGAATGTTGT TGCTGAAACT TTAGCTGCCC TGGAGAGCAA GGAAAGAGAG 
TTGCAACTTT TGAATGAAAA ATTAGAAACT GAGCAGGCAG AGACTGGAGA GTTAAAAAAG 
AACAACCATT TACTTCAGGA ATCTCTAAAG GAGCTACAGT TTTTGTCTGA AACCCTGAGC 
TTGGAGAAAA AGGAACTGAA TTCCATCATT TCTCTAAATA AAAAGGACAT TGAAGAGTTG 
ACCCAAGAGA ATGGGACTCT CAAGGAAATT AATGCCACCT TAACTCAAGA GAAGATGAAC 
TTGCTCCAAA AAACCGAGAG TTTTTCGAAC TGTATAGATG AAAGAGACAG AAGCATTTCA 
GAGTTATCTA ATCAGTACAA ACAAGAAAGG CTTACTTTAC TACAAAGATG TGAAGAAACA 
GGAAATGCAT TTCAGGATCT TAGTGAAAAA TATAAAGCGG TTCAGGAAAA GAACTCTAAA 
TTAGAATGCT TGCTGAGTGA GTGCACGGGT GTTTGTGAAG ACAGAAAAAA TGAATTAGGA 
CAGCTAAAGG AAACATTTGC AAGGGAACAC CAAGCATTTG TATCACAGTT AGCATTAGCT 
GAAGAAAGAA ACCAGAATTT AATTGTGGAG TTGGAGACAG TGCAGCAAGC CCTGCAGTCT 
GAGATTACAG ATATCCAAAA CAGCTCCAAG AGGGAGACTG ATGGTTTAAA GCAAGAAATC 
ATGAATTTAA AGGAAGAACA AAATAAGATG CAACAGGAAG TTAGTGCCTT ATTACAAGAG 
AATGAACACC TGATGGAATT AATGAAGACG AAACATGAGC ATCAATGTCT AGAATTGGAA 
CCAATTCAAG ACTCTGAGAA AGAAGAGAGG GAGATAAATA CATGTCACCT TCAACTTCCG 
ATGGATCTTG ATGTTAAAGA TACTTCTCCA GACACTTGTA ATGCACAGCT GGTGCAAGTA 
GAATCTAAAG TCAGAAACAT GGAATTAAAA CTTCAGAAGA GTGAGAAGGA AGAGTGTCTA 
CAGTATGAGT TACAAACTAG AGAATTAGAA ACTGAAGATT TGCAACAAGA TCCTCAGTCA 
CAAGATATTA GTGGCCTTGG AGACTCTGAA ATAGATCCAG AAGAAAAATA TATTTCAGTG 
CTTCAGGAGT TGTCAACAAG TCAGCAGGAC AATGCCCACC TGCAGTGTTC TCTACAGACA 
GCCATGAACA AGCTGAATGA GTTAGAGAAA ATGTGTGAAG CCCTGCAGGT TGAAAAGTCT 
GAACTCATAT CTGAGCTGAA TGACTCAAGA TCAGAATGTA TCACAGCAAC TAGTAAAATG 
GCAGAAGAGG TAGGGAAACT AGTAAATGAA GTTGAAACAT TAAATGACGA AAATGCACTT 
CTCCAAGGCG AGTTAGTGGA AGAAACACCA GAAGGTGAAT TTGGTGAACA ACAAGATGAG 
CAGACATCTG TGAACTTAAA TCCTTTGGAT GACAGTAATT TCTATGAGCA TTTGACCATG 
TCAAGCAAAG AGGTTCAAAT GCACTTTGCT GAATTACAGG AGAAATTTTC ATCTTTACAG 
AGTGAGCACA AAATTTTACA CGAACAGCAC TGTCTGATGA GCTCTAAGAT GTCAGAGCTG 
CAGTCCTACG TCGACACGTT AAAGGCTGAA AATTCAGTCT TGTCGATGAG TCTGAGAAGC 
TCTCAAGGTG ACTTGGTAAA GGAGGTGCCA CCAGGACCTG GGGAGGAGCA TTTACTGTCC 
TTGTCATTTT CTTGTGTGAC TGACAGCCCT GGTAAGGCAA GTTTGGGAGA GTCCTCTTTT 
TACAAAGATC TTCTAGAGCA CACAACAGAA ACGTCCCTTT TGAATAATCT AGAAGGGACT 
GTTTCGGCAA ACCAGTCCAA TGTAGAGGAA GTCTCTTGTA GTAGTCTGGA GGAGGAGAAT 
CTGACCGAGA AAGAGATCCC CTCTGTCCCA CTGAGGAGCG CTCGAGAACT TGAGACCCTC 
TGTCAGACGT ACCTCGAGTC CCTCAAGCAG CTGGAAGAGG AAATCGAAAG TCAAGGGATT 
ACGAAAAACA AGGAGATCAA AGAGCTCAAG GAGCTACTGA GTTCTTCGAG GGAGGAGCTT 
GACGACCTCA GGAAGCAGTA TTTGTCAGAA AATGAACAGT GGCAGCAGAA GCTGACAGAT 
GTGACAGCGG AGATGGAGTC CAAGTTGGCG GCTGAAAAGA GACACGCAGA GCACCTTACA 
CTTGAGCTTG AGGTGGCGCG GCTTCAGCTG CAGGGTCTGG ACTTAAGCTC TCGGTCTCTG 
CTTGGCACCG ACATAGAAGA TGCTATTCTG GGTGGAAACG ATAGCTGTGA CATAAGAGAA 
TCAGAAGAAT ATATTTCAGA AACTAAAGAG AGGACCCCAA AGCATGAGAT TCACCAGATT 
TGTGAAAAAG ATGTTCAGCA GGACCTCAGT CTAGAGATGG AGAAAATAAC AAAGACTGGT 
GCAACCAAAC TTACAGAAGA ATGGTCCAGA GAGCAGTCCT CAGAAAGCAG TCATGAGACC 
CCAGTGGAAG ATCCAGCCCA GGGCTGTTCA GGATGCATTT CTGAACTGTC CCTTTCTGGG 
CCTAATGCTT CAGTGCCTAG GGATTTTCTG GAGAATCAGG TAACCATTCA GAGTCTCGAG 
CTGAAAGTAA AAGAGACATC AAATGAGAAT TTGAGATTAC TTCATGGCAT AGAGGAGCGT 
GACCAAAAAG TTGAAAACTT GCTAAATGAA ATCAAAGAGT TAGACTCAAA ATTCCATTTA 
CTGGAAGTAC AATTAACTAC CAAGATTGAA GCATGTGTGG CATTGGAAAA AATAGTTGAG 
GAACTTAAGA AAGAAAAATT AGATTTAAAT GAAAAATTGG AATCCTTTTC TTGTCATAAC 
CAGAGAGAAG AAAGTTCAGG AGGTCTCACT TCCAACTTAG AAATGGTCAC AAGCAAATTT 
CCACATGAAG GTATTGAAGA TGATGTAGCC AAGGTGACTG ACAACTGGAG AGAGAAATGT 
CTCCAGGTGG AAAATGAGCT ACAGAGGATT CAATCTGAGA AAGATAGCAT GGAGCATCAT 
GCCCTCTCTG CTGAAGCCAG CTTAGAGGCA GTTCAAACAG AGAAACTATA TTTAGAAAAA 
GACAATGAAA ATAAACAAAC AGTTATAACC TGTCTTGAGG AAGGACTCTC AGTGGTCACA 
AGTGAGAGAG ATCAGCTTCG TGGAGAGTTA GATACTTTGT CAAAAGAAAA TCAAGAGCTA 
GATCAGATGT CTGAAAAGAT GAAGGAGAAA ATACGGGAGC TTGAATCTCA TCAAAGTGAG 
TGTCTGCATC TCCAAGAGTG GCTTCAGAGT TTGGAAAAGG ACTCACAGGC ACTGTCTCTG 
GTAAGAAGTG AGCTGGAAAA CCAAATTGAA CAACTGAATA AAGAGAAAGA CTCACTTGTA 
TGGGAATCCG AAAGCCTGCA GACCAAACTG AGCGAATCGG AGCATGAAAA GCTGGCCATC 
ACCAAGGCTT TGGAGGCTGC CCTGATGGAG AAGGGTGAGG TCGCGGTGAG GCTGAGCTCC 
ACGCAAGAGG AAGTGCACCA GTTGAGAAAA GGCATTGAGA AACTCAGGGT CCGCATTGAG 
GCTGATGAAA AGAAGCAGCT CCATGTCTCA GAGAAACTGA AAGAAAGCGA GCGGAGGAAT 
GACTCGCTTC AGGACAAGGT TGAGACCCTT GAAAGGGAGT TGCAGATGGC AGAAGAAAAC 
CAAGAGCTGG TGATTCTTGA TGCTGAGAAT TGCAAAGCAG AGGTGGAAAC CCTAAAAACA 
CAAATAGAAT TGATGACTGA ACGCCTAAAA GATTTAGAAT TAGACCTTGT CACAATACGG 
TCTGAAAAAG AAAATCTTTT GAAACAGCTA CAAGAGAAAC AAGGTCAGGC GTCTGAATTA 
GATACGCTAC TCTCTTCATT GAAAAACCTA TTAGAAGAAA AGGAGCGAGA GAAAATACAG 
ACGAAAGAAG AAGCTAAAAG TGCAGTGGAG ATGCTGCAAA CACAGTTGAG AGAGCTAACT 
GAGGAAATAG CAGCCTTATG TGATGACCAG GAGACATGGA AGGTCGAAGA ACAGAGTCTG 
GACTCTCCTG CCCAGGAGGT ACAACAGCTG AGAAATAACA TTGAAAAGCT GAAAGTCCAC 
TTGGACATTG ATAAAAAGAA GCAGCTCCAA ATCCTAGAAA AACTGAAGGA AAGTGAGCAT 
CAGGCCGATT TCCTTAAGGA TACAGTTGAG AATCTTGAAA GAGAACTCAA GCTATCAGGA 
GAAAACCAAG AGCATGTGAC TCTTGAGGCT GAGAAGTCCA AAGCAGAAGT AGAGACCCTG 
AAAGCAACAG TGGAGAAGAT GGACCAAAAT CTGAGAGGTT TGCAATTAGA TCTTGTCAAT 
ATAAGATCAG AAAAAGAAGA TCTGACTAAA GAATTACAGA AAGAGCAGAG TCGAGTATCT 
GAATTAGAAA CGTTAAATTC TTCATTTGAA AACCTATTGC GGGAAAAAGA GCAAGAAAAA 
GTACAGATGA AGGAAGAATC TAAAGCTGCA GTGGAGATGC TGCAAACACA GCTGAAAGAG 
CTAAGTGAAG AAGTAGCAAT CTTGTGTGTT GACCAGGAGA CCTGGAAGGT TGAAGAACAG 
AGTCTGGACT CCCCACTTGA GGAGATACAA CAGCTGAGAA ATAACATTGG AAAGCTGAAA 
GTCTGCCTAG ATGCTGATAA AAAGCAGCAG CTCCATATCT TAGAAAAACT GAAGGAAAGT 
GAGCATCAGG CCGATTTCCT TAAGGATACA GTTGAGAACC TTGAAAGAGA ACTCAAGCTA 
TCAGGAGAAA ACCAAGAGCA TGTGACTCTT GAGGCTGAGA AGTCCAAAGC AGAAGTAGAG 
ACCCTGAAAG CAACAGTGGA GGAGATGGAC CAAAATCTGA GAGGTTTGCA ATTAGATCTT 
GTCAATATAA GATCAGACAA AGAAGATCTG ACTAAAGAAT TACAGAAAGA GCAGAGTCGA 
GTGTCTGAAT TAGAAACGTT AAATTCTTCA TTTGAAAACC TATTGCGGGA AAAAGAGCAA 
GAAAAAGTAC AGATGAAGGA AGAATCTAAA GCTGCAGTGG AGATGCTGCA AAAAGAATTA 
GAAGAGCTAA ACGAGAAAAT GGCAGCCTTG GGTAATGACC AAGAGACCCG GAAGGTCAAA 
GAACAGAGCC TGAGTAGTCA AGTAGATTCC CTTGAACATG AGAAGGCTCA GTTGCTGCAG 
GACCTTGATG ATGCCAAAAG TAATTACATG ATTTTGCAGT CTTCTGTGAA TGACCTCATT 
CAAGAAGTTG AAGATGGCAA ACAGAAACTA GAGAAAAAGG ATGAAGAAAT CAGTATACTA 
AAAAGTCAGA CTCGGGACCA AGAGCAGCTT GTCTCTAAAC TGTCCCAGAT GGAAGGAGAA 
CAGCAACTTT GGAAGAAGCA AAAGGCAGAC CTGGAAAATC TGATGGTGGA ATTGGAGCAG 
AAGATTCAGG TGCTGCAATC CAAAAACGAT GCTTTGCAGG ACACCTTAGA AGCTCTGCAG 
AATTCTTCCA GGAATCTGGA GAAAGAGCTT GAATTGACAA AATTGGAAAA AATGTCCTTT 
GTTGAAAAAG TAAACACAAT GACTGTGAAG GAAACTGAGC TTCAGAAGGA AATACATGCA 
GTGATACAAA AAACGGTGGC ACTGAAAGAA GAATTTAGTG GGGAGAAAAA CAGGCTAATG 
GAAGAATTAA ATTTAATGTT GGAAGAAGTG AAGAGCAGCA AAGGTCAACT GAAGGAGCTC 
ATGCTAGAAA ATAGTGAATT GAAGAAGAGT TTGGATTGTG TACACAAAGA CCGTATGGAA 
GAACAAGGGA AAATGAGAGG AGAAATAGCT GAATATCAGC TACGGCTTCA GGAAGCTGAA 
AACAAACATC AGGCTTTGCT TCTAGATACA AACAAACAGC ATGAAATGGA AATCCAGACA 
TACCGAGAGA AACTGACTTC TAAGGAGGAA TGTCTCAGCT CACAGAAGGT GGAGATAGAC 
CTCTTAAAGT CCAGTAAAGA AGAACTCAAT AATTCTTTGA AAGCGACCAC TGAGATTTTA 
GAAGAATTGA AGAAAACCAA GATGGAAAAT CTAAAACATG CAGATAAATT GAAGAAGGAA 
AACGATCGTG CCCAGAGTAA AATAAAGTTG TTGGTGAAAT CCTGTAAACA GCTGGAAGAG 
GAAAAGGTGA TGTTGCAGAA AGAACTATCT CATCTTGAAG CTGCACAGGA GAAACAGAGA 
GCAGATACCG TCGTGGATGC TAATGTCGAC GAATTAATAA CTGAAATGAA AGAACTGAAA 
GAAACCCTTG AAGAAAAAAC CAAGGAGGCA GACGAATACC TGGATAAATA CTGTTCCCTG 
CTCATAAGCC ATGAGAAGTT AGAGAAAGCC AAGGAGATGT TAGAAACACA AGTTGCTCGT 
CTGAGTTCAC AACAATCTAA ACTGAATCTT CGGAGATCTC CTTTGGTGAA TTCGCTTGCT 
CCAGGACCAT CCCCAGTCCC TTCTGCCACT GAGAAGAAGT TACCATCCGG CCAAAATAAA 
TCTTCAGGCA AAAGGCAAAG GTCCAGTGGG ATTCGGGAGG ATGGCGGAGA AACCACGCCT 
TCTACACCAG AGACATTTTC TAAAAAAAGC AGGAAAGCCA TCAGAAGTGG TACTCACCTT 
GCAGAGGATG CAGAAGATGC TGAATTTGAG CCAGAGGGGC TTCCAGAAGT TGTAAAGAAA 
GGGTTTGCCG ACATCCCAAC CGGAAAGACT AGCCCCTACA TCCTGCGGAG GACAACCATG 
GCAACCAGGA CCAGCCCCCG CCTTGCTGCC CAGAAGTTAG CAGCATCCCC GCCAAGTCTC 
GACAAAGAAA ATCTCACCGA GACCTCCAAA CCAACAGCTG GTGGCAGCAG ATCACAAAAG 
GTCAAGGTTT CTCAACAGAG CCCAGTGGAT TCAGGCACTG CCTTCCGAGA TCCCATTGGC 
AGATCTCTCT CCATCAGTAA TCTTCCCGAG AGAAGTCCAG TGGGCAGCCC CAGGGAGGGC 
CTGAGAGCCA AGCGAGGCCG CCACGCCCCC AGCCCAGAAT CCAGCCTGGA GTCCAAGGGC 
AGCGAGAACT GTAGGGTCCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CENPF ( XM_005217369.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005217426.1 Bos taurus centromere protein F (CENPF), transcript variant X1, mRNA.

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CloneID	OBa27521
Clone ID Related Accession (Same CDS sequence)	XM_005217369.4 , XM_005217369.3
Accession Version	XM_005217369.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	centromere protein F isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005217369.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGGG CTTTGGAAGA ATGGAAGGAA GGCTTGCCTA CAAGAGCTCT TCAGAAGATT 
CAAGAGCTTG AAGGCCAGCT CGACAAACTG AAGAAAGAAA GGCAGCAACG GCAGTTTCAG 
CTTGAGACCC TGGAGGCTGC GTTGCAGAAG CAAAAACAGA AGGTTGAGAA TGAAAAAACT 
GAGGGTGCAA ACCTGAAAAG GGAGAACCAA AGCTTGATGG AAATATGTGA AAATCTGGAG 
AAGACTAAGC AGAAGATTTC TCATGAACTT CAAGTCAAGG AGTCACAAGT GAATTTCCAA 
GAAGGTCAAC TGAACTCAAG CAAAAAACAA ATAGAAAAGC TGGAACAGGA ACTTAAAAGG 
TGTAAATCTG AACTTGAAAG AAGCCAACAA GCTGCACAGT CTGCGGATGT CTCTCTGAAT 
TCATGCCTTA CACCACAAAA AATCTTTGCA ACTCCGCTCA CACCAAGCCA GTATTATAGC 
GGTTCCAAAT ATGAAGATCT AAAAGAAAAA TATAATAAAG AAGTTGAAGA ACGAAAAAGA 
TTAGAGGCAG AGGTAAAAGC CTTGCAGGCA AAGAAAGCAA GCCAGACTAT TCCCCAAAGC 
ACCATGAATC ACCGGGACAT CGCCCGACAT CAGGCTTCTT CATCTGTGTT CTCATGGCAA 
CAAGAAAAAA CCCCAAGCCG TCTTTCATCT AACACTCTGA AAACTCCAGT TAGGAGAGAT 
TTCTCTGCAT CTCATTTTTC TGGGGAACAA GAAGTGACTC CGAGTAGATC AACTTTGCAA 
ATAGGGAAAA CAGATGCTAA TAGCAGTTTC TGTGATAATT CTAGCAATTC TCATCTTTTG 
GACCAGTTAA AAGCACAGAA CCAAGAACTA AGGAGCAAGA TGAGTGAGCT GGAACTACGT 
CTGCAAGGAC AAGAAAAAGA AATGAAAGGC CAAGTGAATA AACTTCAAGA ACTCCAACTG 
CAACTGGAGA AAGCAAAAGT GGAATTAAAT GAAAAAGAGA AAGTTTTGAA CAAAAACCGG 
GATGAACTAG TGAGAACAAC TTCACAGTAT GACCAGGCAT CAGCCAAGTG TACTGCTTTG 
GAACAAAAAT TGAAAAAATT GACTGAAGAT TTGAGTTGTC AGCGTCAAAA TGCAGAAAGT 
GCCAGATGTT CTCTGGAACA GAAAATCAAG GAGAAAGAAA AAGAGTTCCA AGAGGAGCTC 
TCCCGTCAAC AGCGTTCCTT CCAAACCCTG GACCAGGAGT GCACTCAGAT GAAAGCCAGG 
CTCACCCAGG AGCTACAGCA AGCTAAGAAC ACATACAACA TCCTACAGGC TGAACTGGAT 
AAAGTCACAT CTGTAAAGCA ACAGATAGAA AAAAAATTGG AAGAGTTTAA GCAAAATTTC 
AGCAAAACTG AACAGGCCTT ACAGGCAAGT CAGACCAAGG AAAATGAGCT GAGGAGAAGC 
AGTGAGGAAA TGAAGAGGGA AAACAGCCTC CTCAAGAGTC AGTCTGAGCA AAGGGCCCGA 
GAAGTCTGCC ACCTGGAGGA AGAACTGAAG AAGGCCAAGC AGTGTTTGAG TCAGAGCCAG 
AATTTTGCAG AAGAAATGAA GGCTAAGAAT ACCTCTCAGG AAACCATGTT AAGAGATCTT 
CAAGAAAAGA TAAATCAGCA AGAGAATTCC TTGACTTTAG AGAAACTGAA GCTTGCCTTG 
GCTGACCTGG AAAAGCAGCG AGATTGTTCT CAAGACCTTT TGAAGAAAAG GGAACATCAC 
ATTGAACAAC TGAACGAAAA GTTAAGCAAA ACAGAGAGAG AATCTGAAGC ATTGTTGAGT 
GCTTTGGAAT TAAAGAAGAA AGAATATGAA GAATTAAAAG AAGAGAAAAC TCTGTTTTCT 
CGCTGGAAAA GTGAAAACGA ACAACTTTTA AATCAGATGG AATCAGAAAA AGAAAGCTTG 
CAGAGTAAAG TTAATCACTT GGAAACTTGT CTTAAGACAC AACAAATAAA AAGTCATGAA 
TATAATGAGA GGGTAAGAAC TCTGGAGATG GAAAGAGAAA ATCTAAACGT GGAGATCAGG 
AACCTTCAAA ATGTGATTGA CAGCAAAACC GCAGAGGCAG AGACACAGAA ACGAGCTTAT 
GGAGAACTGC AACAGAAAGC TGAGTTCTCA GATCAGAAAC ACGAGAAGGA GATAGAAAAT 
ATGTGTTTGA AAATCTCTCA CCTCACTGGG CAAGTGGAAG ACCTAGAACA TAAGCTTCAG 
TTACTGTCAA GTGAAATCAT GGACAAAGAC CAGCGTTACC AAGACTTGCA TGCTGAATCT 
GAGAGCCTCA GGGATCTGCT AAAATCCAGA GATTCCTCTG CGATGACAAT TGAGCCTCAT 
CAGAGAAGTC GTTTAGCTTT TGAACAGCAG TCTGCACTGA ACAATTCCTT TGCAAATATA 
ATCGGAGAGC AAGAAAGTGT GCCTTCAGAA AGGAGCAGAT GCCATGTAGC AACAGACCAA 
AGTCCGAAAA GTTCATCCAT GTTGCAAAAT AGAGTTGTTT CTCTTGAATT TTCCTTAGAG 
TCGCAAAAGC AGATGAACTC AGATCTGCAG AAGCAGTGTG AAGAGTTGGT GCAAATCAAA 
GGAGAAATTG AAGAAAACCT CATGAAAGCA GAACAGATGC ATCAAAGTTT TGTGGCTGAA 
ACAAGCCAAC GCATTAGTAA GTTACAAGAA GACACTTCTG TTCATCAGAA TGTTGTTGCT 
GAAACTTTAG CTGCCCTGGA GAGCAAGGAA AGAGAGTTGC AACTTTTGAA TGAAAAATTA 
GAAACTGAGC AGGCAGAGAC TGGAGAGTTA AAAAAGAACA ACCATTTACT TCAGGAATCT 
CTAAAGGAGC TACAGTTTTT GTCTGAAACC CTGAGCTTGG AGAAAAAGGA ACTGAATTCC 
ATCATTTCTC TAAATAAAAA GGACATTGAA GAGTTGACCC AAGAGAATGG GACTCTCAAG 
GAAATTAATG CCACCTTAAC TCAAGAGAAG ATGAACTTGC TCCAAAAAAC CGAGAGTTTT 
TCGAACTGTA TAGATGAAAG AGACAGAAGC ATTTCAGAGT TATCTAATCA GTACAAACAA 
GAAAGGCTTA CTTTACTACA AAGATGTGAA GAAACAGGAA ATGCATTTCA GGATCTTAGT 
GAAAAATATA AAGCGGTTCA GGAAAAGAAC TCTAAATTAG AATGCTTGCT GAGTGAGTGC 
ACGGGTGTTT GTGAAGACAG AAAAAATGAA TTAGGACAGC TAAAGGAAAC ATTTGCAAGG 
GAACACCAAG CATTTGTATC ACAGTTAGCA TTAGCTGAAG AAAGAAACCA GAATTTAATT 
GTGGAGTTGG AGACAGTGCA GCAAGCCCTG CAGTCTGAGA TTACAGATAT CCAAAACAGC 
TCCAAGAGGG AGACTGATGG TTTAAAGCAA GAAATCATGA ATTTAAAGGA AGAACAAAAT 
AAGATGCAAC AGGAAGTTAG TGCCTTATTA CAAGAGAATG AACACCTGAT GGAATTAATG 
AAGACGAAAC ATGAGCATCA ATGTCTAGAA TTGGAACCAA TTCAAGACTC TGAGAAAGAA 
GAGAGGGAGA TAAATACATG TCACCTTCAA CTTCCGATGG ATCTTGATGT TAAAGATACT 
TCTCCAGACA CTTGTAATGC ACAGCTGGTG CAAGTAGAAT CTAAAGTCAG AAACATGGAA 
TTAAAACTTC AGAAGAGTGA GAAGGAAGAG TGTCTACAGT ATGAGTTACA AACTAGAGAA 
TTAGAAACTG AAGATTTGCA ACAAGATCCT CAGTCACAAG ATATTAGTGG CCTTGGAGAC 
TCTGAAATAG ATCCAGAAGA AAAATATATT TCAGTGCTTC AGGAGTTGTC AACAAGTCAG 
CAGGACAATG CCCACCTGCA GTGTTCTCTA CAGACAGCCA TGAACAAGCT GAATGAGTTA 
GAGAAAATGT GTGAAGCCCT GCAGGTTGAA AAGTCTGAAC TCATATCTGA GCTGAATGAC 
TCAAGATCAG AATGTATCAC AGCAACTAGT AAAATGGCAG AAGAGGTAGG GAAACTAGTA 
AATGAAGTTG AAACATTAAA TGACGAAAAT GCACTTCTCC AAGGCGAGTT AGTGGAAGAA 
ACACCAGAAG GTGAATTTGG TGAACAACAA GATGAGCAGA CATCTGTGAA CTTAAATCCT 
TTGGATGACA GTAATTTCTA TGAGCATTTG ACCATGTCAA GCAAAGAGGT TCAAATGCAC 
TTTGCTGAAT TACAGGAGAA ATTTTCATCT TTACAGAGTG AGCACAAAAT TTTACACGAA 
CAGCACTGTC TGATGAGCTC TAAGATGTCA GAGCTGCAGT CCTACGTCGA CACGTTAAAG 
GCTGAAAATT CAGTCTTGTC GATGAGTCTG AGAAGCTCTC AAGGTGACTT GGTAAAGGAG 
GTGCCACCAG GACCTGGGGA GGAGCATTTA CTGTCCTTGT CATTTTCTTG TGTGACTGAC 
AGCCCTGGTA AGGCAAGTTT GGGAGAGTCC TCTTTTTACA AAGATCTTCT AGAGCACACA 
ACAGAAACGT CCCTTTTGAA TAATCTAGAA GGGACTGTTT CGGCAAACCA GTCCAATGTA 
GAGGAAGTCT CTTGTAGTAG TCTGGAGGAG GAGAATCTGA CCGAGAAAGA GATCCCCTCT 
GTCCCACTGA GGAGCGCTCG AGAACTTGAG ACCCTCTGTC AGACGTACCT CGAGTCCCTC 
AAGCAGCTGG AAGAGGAAAT CGAAAGTCAA GGGATTACGA AAAACAAGGA GATCAAAGAG 
CTCAAGGAGC TACTGAGTTC TTCGAGGGAG GAGCTTGACG ACCTCAGGAA GCAGTATTTG 
TCAGAAAATG AACAGTGGCA GCAGAAGCTG ACAGATGTGA CAGCGGAGAT GGAGTCCAAG 
TTGGCGGCTG AAAAGAGACA CGCAGAGCAC CTTACACTTG AGCTTGAGGT GGCGCGGCTT 
CAGCTGCAGG GTCTGGACTT AAGCTCTCGG TCTCTGCTTG GCACCGACAT AGAAGATGCT 
ATTCTGGGTG GAAACGATAG CTGTGACATA AGAGAATCAG AAGAATATAT TTCAGAAACT 
AAAGAGAGGA CCCCAAAGCA TGAGATTCAC CAGATTTGTG AAAAAGATGT TCAGCAGGAC 
CTCAGTCTAG AGATGGAGAA AATAACAAAG ACTGGTGCAA CCAAACTTAC AGAAGAATGG 
TCCAGAGAGC AGTCCTCAGA AAGCAGTCAT GAGACCCCAG TGGAAGATCC AGCCCAGGGC 
TGTTCAGGAT GCATTTCTGA ACTGTCCCTT TCTGGGCCTA ATGCTTCAGT GCCTAGGGAT 
TTTCTGGAGA ATCAGGTAAC CATTCAGAGT CTCGAGCTGA AAGTAAAAGA GACATCAAAT 
GAGAATTTGA GATTACTTCA TGGCATAGAG GAGCGTGACC AAAAAGTTGA AAACTTGCTA 
AATGAAATCA AAGAGTTAGA CTCAAAATTC CATTTACTGG AAGTACAATT AACTACCAAG 
ATTGAAGCAT GTGTGGCATT GGAAAAAATA GTTGAGGAAC TTAAGAAAGA AAAATTAGAT 
TTAAATGAAA AATTGGAATC CTTTTCTTGT CATAACCAGA GAGAAGAAAG TTCAGGAGGT 
CTCACTTCCA ACTTAGAAAT GGTCACAAGC AAATTTCCAC ATGAAGGTAT TGAAGATGAT 
GTAGCCAAGG TGACTGACAA CTGGAGAGAG AAATGTCTCC AGGTGGAAAA TGAGCTACAG 
AGGATTCAAT CTGAGAAAGA TAGCATGGAG CATCATGCCC TCTCTGCTGA AGCCAGCTTA 
GAGGCAGTTC AAACAGAGAA ACTATATTTA GAAAAAGACA ATGAAAATAA ACAAACAGTT 
ATAACCTGTC TTGAGGAAGG ACTCTCAGTG GTCACAAGTG AGAGAGATCA GCTTCGTGGA 
GAGTTAGATA CTTTGTCAAA AGAAAATCAA GAGCTAGATC AGATGTCTGA AAAGATGAAG 
GAGAAAATAC GGGAGCTTGA ATCTCATCAA AGTGAGTGTC TGCATCTCCA AGAGTGGCTT 
CAGAGTTTGG AAAAGGACTC ACAGGCACTG TCTCTGGTAA GAAGTGAGCT GGAAAACCAA 
ATTGAACAAC TGAATAAAGA GAAAGACTCA CTTGTATGGG AATCCGAAAG CCTGCAGACC 
AAACTGAGCG AATCGGAGCA TGAAAAGCTG GCCATCACCA AGGCTTTGGA GGCTGCCCTG 
ATGGAGAAGG GTGAGGTCGC GGTGAGGCTG AGCTCCACGC AAGAGGAAGT GCACCAGTTG 
AGAAAAGGCA TTGAGAAACT CAGGGTCCGC ATTGAGGCTG ATGAAAAGAA GCAGCTCCAT 
GTCTCAGAGA AACTGAAAGA AAGCGAGCGG AGGAATGACT CGCTTCAGGA CAAGGTTGAG 
ACCCTTGAAA GGGAGTTGCA GATGGCAGAA GAAAACCAAG AGCTGGTGAT TCTTGATGCT 
GAGAATTGCA AAGCAGAGGT GGAAACCCTA AAAACACAAA TAGAATTGAT GACTGAACGC 
CTAAAAGATT TAGAATTAGA CCTTGTCACA ATACGGTCTG AAAAAGAAAA TCTTTTGAAA 
CAGCTACAAG AGAAACAAGG TCAGGCGTCT GAATTAGATA CGCTACTCTC TTCATTGAAA 
AACCTATTAG AAGAAAAGGA GCGAGAGAAA ATACAGACGA AAGAAGAAGC TAAAAGTGCA 
GTGGAGATGC TGCAAACACA GTTGAGAGAG CTAACTGAGG AAATAGCAGC CTTATGTGAT 
GACCAGGAGA CATGGAAGGT CGAAGAACAG AGTCTGGACT CTCCTGCCCA GGAGGTACAA 
CAGCTGAGAA ATAACATTGA AAAGCTGAAA GTCCACTTGG ACATTGATAA AAAGAAGCAG 
CTCCAAATCC TAGAAAAACT GAAGGAAAGT GAGCATCAGG CCGATTTCCT TAAGGATACA 
GTTGAGAATC TTGAAAGAGA ACTCAAGCTA TCAGGAGAAA ACCAAGAGCA TGTGACTCTT 
GAGGCTGAGA AGTCCAAAGC AGAAGTAGAG ACCCTGAAAG CAACAGTGGA GAAGATGGAC 
CAAAATCTGA GAGGTTTGCA ATTAGATCTT GTCAATATAA GATCAGAAAA AGAAGATCTG 
ACTAAAGAAT TACAGAAAGA GCAGAGTCGA GTATCTGAAT TAGAAACGTT AAATTCTTCA 
TTTGAAAACC TATTGCGGGA AAAAGAGCAA GAAAAAGTAC AGATGAAGGA AGAATCTAAA 
GCTGCAGTGG AGATGCTGCA AACACAGCTG AAAGAGCTAA GTGAAGAAGT AGCAATCTTG 
TGTGTTGACC AGGAGACCTG GAAGGTTGAA GAACAGAGTC TGGACTCCCC ACTTGAGGAG 
ATACAACAGC TGAGAAATAA CATTGGAAAG CTGAAAGTCT GCCTAGATGC TGATAAAAAG 
CAGCAGCTCC ATATCTTAGA AAAACTGAAG GAAAGTGAGC ATCAGGCCGA TTTCCTTAAG 
GATACAGTTG AGAACCTTGA AAGAGAACTC AAGCTATCAG GAGAAAACCA AGAGCATGTG 
ACTCTTGAGG CTGAGAAGTC CAAAGCAGAA GTAGAGACCC TGAAAGCAAC AGTGGAGGAG 
ATGGACCAAA ATCTGAGAGG TTTGCAATTA GATCTTGTCA ATATAAGATC AGACAAAGAA 
GATCTGACTA AAGAATTACA GAAAGAGCAG AGTCGAGTGT CTGAATTAGA AACGTTAAAT 
TCTTCATTTG AAAACCTATT GCGGGAAAAA GAGCAAGAAA AAGTACAGAT GAAGGAAGAA 
TCTAAAGCTG CAGTGGAGAT GCTGCAAAAA GAATTAGAAG AGCTAAACGA GAAAATGGCA 
GCCTTGGGTA ATGACCAAGA GACCCGGAAG GTCAAAGAAC AGAGCCTGAG TAGTCAAGTA 
GATTCCCTTG AACATGAGAA GGCTCAGTTG CTGCAGGACC TTGATGATGC CAAAAGTAAT 
TACATGATTT TGCAGTCTTC TGTGAATGAC CTCATTCAAG AAGTTGAAGA TGGCAAACAG 
AAACTAGAGA AAAAGGATGA AGAAATCAGT ATACTAAAAA GTCAGACTCG GGACCAAGAG 
CAGCTTGTCT CTAAACTGTC CCAGATGGAA GGAGAACAGC AACTTTGGAA GAAGCAAAAG 
GCAGACCTGG AAAATCTGAT GGTGGAATTG GAGCAGAAGA TTCAGGTGCT GCAATCCAAA 
AACGATGCTT TGCAGGACAC CTTAGAAGCT CTGCAGAATT CTTCCAGGAA TCTGGAGAAA 
GAGCTTGAAT TGACAAAATT GGAAAAAATG TCCTTTGTTG AAAAAGTAAA CACAATGACT 
GTGAAGGAAA CTGAGCTTCA GAAGGAAATA CATGCAGTGA TACAAAAAAC GGTGGCACTG 
AAAGAAGAAT TTAGTGGGGA GAAAAACAGG CTAATGGAAG AATTAAATTT AATGTTGGAA 
GAAGTGAAGA GCAGCAAAGG TCAACTGAAG GAGCTCATGC TAGAAAATAG TGAATTGAAG 
AAGAGTTTGG ATTGTGTACA CAAAGACCGT ATGGAAGAAC AAGGGAAAAT GAGAGGAGAA 
ATAGCTGAAT ATCAGCTACG GCTTCAGGAA GCTGAAAACA AACATCAGGC TTTGCTTCTA 
GATACAAACA AACAGCATGA AATGGAAATC CAGACATACC GAGAGAAACT GACTTCTAAG 
GAGGAATGTC TCAGCTCACA GAAGGTGGAG ATAGACCTCT TAAAGTCCAG TAAAGAAGAA 
CTCAATAATT CTTTGAAAGC GACCACTGAG ATTTTAGAAG AATTGAAGAA AACCAAGATG 
GAAAATCTAA AACATGCAGA TAAATTGAAG AAGGAAAACG ATCGTGCCCA GAGTAAAATA 
AAGTTGTTGG TGAAATCCTG TAAACAGCTG GAAGAGGAAA AGGTGATGTT GCAGAAAGAA 
CTATCTCATC TTGAAGCTGC ACAGGAGAAA CAGAGAGCAG ATACCGTCGT GGATGCTAAT 
GTCGACGAAT TAATAACTGA AATGAAAGAA CTGAAAGAAA CCCTTGAAGA AAAAACCAAG 
GAGGCAGACG AATACCTGGA TAAATACTGT TCCCTGCTCA TAAGCCATGA GAAGTTAGAG 
AAAGCCAAGG AGATGTTAGA AACACAAGTT GCTCGTCTGA GTTCACAACA ATCTAAACTG 
AATCTTCGGA GATCTCCTTT GGTGAATTCG CTTGCTCCAG GACCATCCCC AGTCCCTTCT 
GCCACTGAGA AGAAGTTACC ATCCGGCCAA AATAAATCTT CAGGCAAAAG GCAAAGGTCC 
AGTGGGATTC GGGAGGATGG CGGAGAAACC ACGCCTTCTA CACCAGAGAC ATTTTCTAAA 
AAAAGCAGGA AAGCCATCAG AAGTGGTACT CACCTTGCAG AGGATGCAGA AGATGCTGAA 
TTTGAGCCAG AGGGGCTTCC AGAAGTTGTA AAGAAAGGGT TTGCCGACAT CCCAACCGGA 
AAGACTAGCC CCTACATCCT GCGGAGGACA ACCATGGCAA CCAGGACCAG CCCCCGCCTT 
GCTGCCCAGA AGTTAGCAGC ATCCCCGCCA AGTCTCGACA AAGAAAATCT CACCGAGACC 
TCCAAACCAA CAGCTGGTGG CAGCAGATCA CAAAAGGTCA AGGTTTCTCA ACAGAGCCCA 
GTGGATTCAG GCACTGCCTT CCGAGATCCC ATTGGCAGAT CTCTCTCCAT CAGTAATCTT 
CCCGAGAGAA GTCCAGTGGG CAGCCCCAGG GAGGGCCTGA GAGCCAAGCG AGGCCGCCAC 
GCCCCCAGCC CAGAATCCAG CCTGGAGTCC AAGGGCAGCG AGAACTGTAG GGTCCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005217426.1
CDS	199..9978
Translation	MSWALEEWKEGLPTRALQKIQELEGQLDKLKKERQQRQFQLETLEAALQKQKQKVENEKTEGANLKRENQSLMEICENLEKTKQKISHELQVKESQVNFQEGQLNSSKKQIEKLEQELKRCKSELERSQQAAQSADVSLNSCLTPQKIFATPLTPSQYYSGSKYEDLKEKYNKEVEERKRLEAEVKALQAKKASQTIPQSTMNHRDIARHQASSSVFSWQQEKTPSRLSSNTLKTPVRRDFSASHFSGEQEVTPSRSTLQIGKTDANSSFCDNSSNSHLLDQLKAQNQELRSKMSELELRLQGQEKEMKGQVNKLQELQLQLEKAKVELNEKEKVLNKNRDELVRTTSQYDQASAKCTALEQKLKKLTEDLSCQRQNAESARCSLEQKIKEKEKEFQEELSRQQRSFQTLDQECTQMKARLTQELQQAKNTYNILQAELDKVTSVKQQIEKKLEEFKQNFSKTEQALQASQTKENELRRSSEEMKRENSLLKSQSEQRAREVCHLEEELKKAKQCLSQSQNFAEEMKAKNTSQETMLRDLQEKINQQENSLTLEKLKLALADLEKQRDCSQDLLKKREHHIEQLNEKLSKTERESEALLSALELKKKEYEELKEEKTLFSRWKSENEQLLNQMESEKESLQSKVNHLETCLKTQQIKSHEYNERVRTLEMERENLNVEIRNLQNVIDSKTAEAETQKRAYGELQQKAEFSDQKHEKEIENMCLKISHLTGQVEDLEHKLQLLSSEIMDKDQRYQDLHAESESLRDLLKSRDSSAMTIEPHQRSRLAFEQQSALNNSFANIIGEQESVPSERSRCHVATDQSPKSSSMLQNRVVSLEFSLESQKQMNSDLQKQCEELVQIKGEIEENLMKAEQMHQSFVAETSQRISKLQEDTSVHQNVVAETLAALESKERELQLLNEKLETEQAETGELKKNNHLLQESLKELQFLSETLSLEKKELNSIISLNKKDIEELTQENGTLKEINATLTQEKMNLLQKTESFSNCIDERDRSISELSNQYKQERLTLLQRCEETGNAFQDLSEKYKAVQEKNSKLECLLSECTGVCEDRKNELGQLKETFAREHQAFVSQLALAEERNQNLIVELETVQQALQSEITDIQNSSKRETDGLKQEIMNLKEEQNKMQQEVSALLQENEHLMELMKTKHEHQCLELEPIQDSEKEEREINTCHLQLPMDLDVKDTSPDTCNAQLVQVESKVRNMELKLQKSEKEECLQYELQTRELETEDLQQDPQSQDISGLGDSEIDPEEKYISVLQELSTSQQDNAHLQCSLQTAMNKLNELEKMCEALQVEKSELISELNDSRSECITATSKMAEEVGKLVNEVETLNDENALLQGELVEETPEGEFGEQQDEQTSVNLNPLDDSNFYEHLTMSSKEVQMHFAELQEKFSSLQSEHKILHEQHCLMSSKMSELQSYVDTLKAENSVLSMSLRSSQGDLVKEVPPGPGEEHLLSLSFSCVTDSPGKASLGESSFYKDLLEHTTETSLLNNLEGTVSANQSNVEEVSCSSLEEENLTEKEIPSVPLRSARELETLCQTYLESLKQLEEEIESQGITKNKEIKELKELLSSSREELDDLRKQYLSENEQWQQKLTDVTAEMESKLAAEKRHAEHLTLELEVARLQLQGLDLSSRSLLGTDIEDAILGGNDSCDIRESEEYISETKERTPKHEIHQICEKDVQQDLSLEMEKITKTGATKLTEEWSREQSSESSHETPVEDPAQGCSGCISELSLSGPNASVPRDFLENQVTIQSLELKVKETSNENLRLLHGIEERDQKVENLLNEIKELDSKFHLLEVQLTTKIEACVALEKIVEELKKEKLDLNEKLESFSCHNQREESSGGLTSNLEMVTSKFPHEGIEDDVAKVTDNWREKCLQVENELQRIQSEKDSMEHHALSAEASLEAVQTEKLYLEKDNENKQTVITCLEEGLSVVTSERDQLRGELDTLSKENQELDQMSEKMKEKIRELESHQSECLHLQEWLQSLEKDSQALSLVRSELENQIEQLNKEKDSLVWESESLQTKLSESEHEKLAITKALEAALMEKGEVAVRLSSTQEEVHQLRKGIEKLRVRIEADEKKQLHVSEKLKESERRNDSLQDKVETLERELQMAEENQELVILDAENCKAEVETLKTQIELMTERLKDLELDLVTIRSEKENLLKQLQEKQGQASELDTLLSSLKNLLEEKEREKIQTKEEAKSAVEMLQTQLRELTEEIAALCDDQETWKVEEQSLDSPAQEVQQLRNNIEKLKVHLDIDKKKQLQILEKLKESEHQADFLKDTVENLERELKLSGENQEHVTLEAEKSKAEVETLKATVEKMDQNLRGLQLDLVNIRSEKEDLTKELQKEQSRVSELETLNSSFENLLREKEQEKVQMKEESKAAVEMLQTQLKELSEEVAILCVDQETWKVEEQSLDSPLEEIQQLRNNIGKLKVCLDADKKQQLHILEKLKESEHQADFLKDTVENLERELKLSGENQEHVTLEAEKSKAEVETLKATVEEMDQNLRGLQLDLVNIRSDKEDLTKELQKEQSRVSELETLNSSFENLLREKEQEKVQMKEESKAAVEMLQKELEELNEKMAALGNDQETRKVKEQSLSSQVDSLEHEKAQLLQDLDDAKSNYMILQSSVNDLIQEVEDGKQKLEKKDEEISILKSQTRDQEQLVSKLSQMEGEQQLWKKQKADLENLMVELEQKIQVLQSKNDALQDTLEALQNSSRNLEKELELTKLEKMSFVEKVNTMTVKETELQKEIHAVIQKTVALKEEFSGEKNRLMEELNLMLEEVKSSKGQLKELMLENSELKKSLDCVHKDRMEEQGKMRGEIAEYQLRLQEAENKHQALLLDTNKQHEMEIQTYREKLTSKEECLSSQKVEIDLLKSSKEELNNSLKATTEILEELKKTKMENLKHADKLKKENDRAQSKIKLLVKSCKQLEEEKVMLQKELSHLEAAQEKQRADTVVDANVDELITEMKELKETLEEKTKEADEYLDKYCSLLISHEKLEKAKEMLETQVARLSSQQSKLNLRRSPLVNSLAPGPSPVPSATEKKLPSGQNKSSGKRQRSSGIREDGGETTPSTPETFSKKSRKAIRSGTHLAEDAEDAEFEPEGLPEVVKKGFADIPTGKTSPYILRRTTMATRTSPRLAAQKLAASPPSLDKENLTETSKPTAGGSRSQKVKVSQQSPVDSGTAFRDPIGRSLSISNLPERSPVGSPREGLRAKRGRHAPSPESSLESKGSENCRVQ

Target ORF information:

RefSeq Version	XM_005217369.4
Organism	Bos taurus(cattle)
Definition	Bos taurus centromere protein F (CENPF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005217369.4

ORF Insert Sequence:

ATGAGCTGGG CTTTGGAAGA ATGGAAGGAA GGCTTGCCTA CAAGAGCTCT TCAGAAGATT 
CAAGAGCTTG AAGGCCAGCT CGACAAACTG AAGAAAGAAA GGCAGCAACG GCAGTTTCAG 
CTTGAGACCC TGGAGGCTGC GTTGCAGAAG CAAAAACAGA AGGTTGAGAA TGAAAAAACT 
GAGGGTGCAA ACCTGAAAAG GGAGAACCAA AGCTTGATGG AAATATGTGA AAATCTGGAG 
AAGACTAAGC AGAAGATTTC TCATGAACTT CAAGTCAAGG AGTCACAAGT GAATTTCCAA 
GAAGGTCAAC TGAACTCAAG CAAAAAACAA ATAGAAAAGC TGGAACAGGA ACTTAAAAGG 
TGTAAATCTG AACTTGAAAG AAGCCAACAA GCTGCACAGT CTGCGGATGT CTCTCTGAAT 
TCATGCCTTA CACCACAAAA AATCTTTGCA ACTCCGCTCA CACCAAGCCA GTATTATAGC 
GGTTCCAAAT ATGAAGATCT AAAAGAAAAA TATAATAAAG AAGTTGAAGA ACGAAAAAGA 
TTAGAGGCAG AGGTAAAAGC CTTGCAGGCA AAGAAAGCAA GCCAGACTAT TCCCCAAAGC 
ACCATGAATC ACCGGGACAT CGCCCGACAT CAGGCTTCTT CATCTGTGTT CTCATGGCAA 
CAAGAAAAAA CCCCAAGCCG TCTTTCATCT AACACTCTGA AAACTCCAGT TAGGAGAGAT 
TTCTCTGCAT CTCATTTTTC TGGGGAACAA GAAGTGACTC CGAGTAGATC AACTTTGCAA 
ATAGGGAAAA CAGATGCTAA TAGCAGTTTC TGTGATAATT CTAGCAATTC TCATCTTTTG 
GACCAGTTAA AAGCACAGAA CCAAGAACTA AGGAGCAAGA TGAGTGAGCT GGAACTACGT 
CTGCAAGGAC AAGAAAAAGA AATGAAAGGC CAAGTGAATA AACTTCAAGA ACTCCAACTG 
CAACTGGAGA AAGCAAAAGT GGAATTAAAT GAAAAAGAGA AAGTTTTGAA CAAAAACCGG 
GATGAACTAG TGAGAACAAC TTCACAGTAT GACCAGGCAT CAGCCAAGTG TACTGCTTTG 
GAACAAAAAT TGAAAAAATT GACTGAAGAT TTGAGTTGTC AGCGTCAAAA TGCAGAAAGT 
GCCAGATGTT CTCTGGAACA GAAAATCAAG GAGAAAGAAA AAGAGTTCCA AGAGGAGCTC 
TCCCGTCAAC AGCGTTCCTT CCAAACCCTG GACCAGGAGT GCACTCAGAT GAAAGCCAGG 
CTCACCCAGG AGCTACAGCA AGCTAAGAAC ACATACAACA TCCTACAGGC TGAACTGGAT 
AAAGTCACAT CTGTAAAGCA ACAGATAGAA AAAAAATTGG AAGAGTTTAA GCAAAATTTC 
AGCAAAACTG AACAGGCCTT ACAGGCAAGT CAGACCAAGG AAAATGAGCT GAGGAGAAGC 
AGTGAGGAAA TGAAGAGGGA AAACAGCCTC CTCAAGAGTC AGTCTGAGCA AAGGGCCCGA 
GAAGTCTGCC ACCTGGAGGA AGAACTGAAG AAGGCCAAGC AGTGTTTGAG TCAGAGCCAG 
AATTTTGCAG AAGAAATGAA GGCTAAGAAT ACCTCTCAGG AAACCATGTT AAGAGATCTT 
CAAGAAAAGA TAAATCAGCA AGAGAATTCC TTGACTTTAG AGAAACTGAA GCTTGCCTTG 
GCTGACCTGG AAAAGCAGCG AGATTGTTCT CAAGACCTTT TGAAGAAAAG GGAACATCAC 
ATTGAACAAC TGAACGAAAA GTTAAGCAAA ACAGAGAGAG AATCTGAAGC ATTGTTGAGT 
GCTTTGGAAT TAAAGAAGAA AGAATATGAA GAATTAAAAG AAGAGAAAAC TCTGTTTTCT 
CGCTGGAAAA GTGAAAACGA ACAACTTTTA AATCAGATGG AATCAGAAAA AGAAAGCTTG 
CAGAGTAAAG TTAATCACTT GGAAACTTGT CTTAAGACAC AACAAATAAA AAGTCATGAA 
TATAATGAGA GGGTAAGAAC TCTGGAGATG GAAAGAGAAA ATCTAAACGT GGAGATCAGG 
AACCTTCAAA ATGTGATTGA CAGCAAAACC GCAGAGGCAG AGACACAGAA ACGAGCTTAT 
GGAGAACTGC AACAGAAAGC TGAGTTCTCA GATCAGAAAC ACGAGAAGGA GATAGAAAAT 
ATGTGTTTGA AAATCTCTCA CCTCACTGGG CAAGTGGAAG ACCTAGAACA TAAGCTTCAG 
TTACTGTCAA GTGAAATCAT GGACAAAGAC CAGCGTTACC AAGACTTGCA TGCTGAATCT 
GAGAGCCTCA GGGATCTGCT AAAATCCAGA GATTCCTCTG CGATGACAAT TGAGCCTCAT 
CAGAGAAGTC GTTTAGCTTT TGAACAGCAG TCTGCACTGA ACAATTCCTT TGCAAATATA 
ATCGGAGAGC AAGAAAGTGT GCCTTCAGAA AGGAGCAGAT GCCATGTAGC AACAGACCAA 
AGTCCGAAAA GTTCATCCAT GTTGCAAAAT AGAGTTGTTT CTCTTGAATT TTCCTTAGAG 
TCGCAAAAGC AGATGAACTC AGATCTGCAG AAGCAGTGTG AAGAGTTGGT GCAAATCAAA 
GGAGAAATTG AAGAAAACCT CATGAAAGCA GAACAGATGC ATCAAAGTTT TGTGGCTGAA 
ACAAGCCAAC GCATTAGTAA GTTACAAGAA GACACTTCTG TTCATCAGAA TGTTGTTGCT 
GAAACTTTAG CTGCCCTGGA GAGCAAGGAA AGAGAGTTGC AACTTTTGAA TGAAAAATTA 
GAAACTGAGC AGGCAGAGAC TGGAGAGTTA AAAAAGAACA ACCATTTACT TCAGGAATCT 
CTAAAGGAGC TACAGTTTTT GTCTGAAACC CTGAGCTTGG AGAAAAAGGA ACTGAATTCC 
ATCATTTCTC TAAATAAAAA GGACATTGAA GAGTTGACCC AAGAGAATGG GACTCTCAAG 
GAAATTAATG CCACCTTAAC TCAAGAGAAG ATGAACTTGC TCCAAAAAAC CGAGAGTTTT 
TCGAACTGTA TAGATGAAAG AGACAGAAGC ATTTCAGAGT TATCTAATCA GTACAAACAA 
GAAAGGCTTA CTTTACTACA AAGATGTGAA GAAACAGGAA ATGCATTTCA GGATCTTAGT 
GAAAAATATA AAGCGGTTCA GGAAAAGAAC TCTAAATTAG AATGCTTGCT GAGTGAGTGC 
ACGGGTGTTT GTGAAGACAG AAAAAATGAA TTAGGACAGC TAAAGGAAAC ATTTGCAAGG 
GAACACCAAG CATTTGTATC ACAGTTAGCA TTAGCTGAAG AAAGAAACCA GAATTTAATT 
GTGGAGTTGG AGACAGTGCA GCAAGCCCTG CAGTCTGAGA TTACAGATAT CCAAAACAGC 
TCCAAGAGGG AGACTGATGG TTTAAAGCAA GAAATCATGA ATTTAAAGGA AGAACAAAAT 
AAGATGCAAC AGGAAGTTAG TGCCTTATTA CAAGAGAATG AACACCTGAT GGAATTAATG 
AAGACGAAAC ATGAGCATCA ATGTCTAGAA TTGGAACCAA TTCAAGACTC TGAGAAAGAA 
GAGAGGGAGA TAAATACATG TCACCTTCAA CTTCCGATGG ATCTTGATGT TAAAGATACT 
TCTCCAGACA CTTGTAATGC ACAGCTGGTG CAAGTAGAAT CTAAAGTCAG AAACATGGAA 
TTAAAACTTC AGAAGAGTGA GAAGGAAGAG TGTCTACAGT ATGAGTTACA AACTAGAGAA 
TTAGAAACTG AAGATTTGCA ACAAGATCCT CAGTCACAAG ATATTAGTGG CCTTGGAGAC 
TCTGAAATAG ATCCAGAAGA AAAATATATT TCAGTGCTTC AGGAGTTGTC AACAAGTCAG 
CAGGACAATG CCCACCTGCA GTGTTCTCTA CAGACAGCCA TGAACAAGCT GAATGAGTTA 
GAGAAAATGT GTGAAGCCCT GCAGGTTGAA AAGTCTGAAC TCATATCTGA GCTGAATGAC 
TCAAGATCAG AATGTATCAC AGCAACTAGT AAAATGGCAG AAGAGGTAGG GAAACTAGTA 
AATGAAGTTG AAACATTAAA TGACGAAAAT GCACTTCTCC AAGGCGAGTT AGTGGAAGAA 
ACACCAGAAG GTGAATTTGG TGAACAACAA GATGAGCAGA CATCTGTGAA CTTAAATCCT 
TTGGATGACA GTAATTTCTA TGAGCATTTG ACCATGTCAA GCAAAGAGGT TCAAATGCAC 
TTTGCTGAAT TACAGGAGAA ATTTTCATCT TTACAGAGTG AGCACAAAAT TTTACACGAA 
CAGCACTGTC TGATGAGCTC TAAGATGTCA GAGCTGCAGT CCTACGTCGA CACGTTAAAG 
GCTGAAAATT CAGTCTTGTC GATGAGTCTG AGAAGCTCTC AAGGTGACTT GGTAAAGGAG 
GTGCCACCAG GACCTGGGGA GGAGCATTTA CTGTCCTTGT CATTTTCTTG TGTGACTGAC 
AGCCCTGGTA AGGCAAGTTT GGGAGAGTCC TCTTTTTACA AAGATCTTCT AGAGCACACA 
ACAGAAACGT CCCTTTTGAA TAATCTAGAA GGGACTGTTT CGGCAAACCA GTCCAATGTA 
GAGGAAGTCT CTTGTAGTAG TCTGGAGGAG GAGAATCTGA CCGAGAAAGA GATCCCCTCT 
GTCCCACTGA GGAGCGCTCG AGAACTTGAG ACCCTCTGTC AGACGTACCT CGAGTCCCTC 
AAGCAGCTGG AAGAGGAAAT CGAAAGTCAA GGGATTACGA AAAACAAGGA GATCAAAGAG 
CTCAAGGAGC TACTGAGTTC TTCGAGGGAG GAGCTTGACG ACCTCAGGAA GCAGTATTTG 
TCAGAAAATG AACAGTGGCA GCAGAAGCTG ACAGATGTGA CAGCGGAGAT GGAGTCCAAG 
TTGGCGGCTG AAAAGAGACA CGCAGAGCAC CTTACACTTG AGCTTGAGGT GGCGCGGCTT 
CAGCTGCAGG GTCTGGACTT AAGCTCTCGG TCTCTGCTTG GCACCGACAT AGAAGATGCT 
ATTCTGGGTG GAAACGATAG CTGTGACATA AGAGAATCAG AAGAATATAT TTCAGAAACT 
AAAGAGAGGA CCCCAAAGCA TGAGATTCAC CAGATTTGTG AAAAAGATGT TCAGCAGGAC 
CTCAGTCTAG AGATGGAGAA AATAACAAAG ACTGGTGCAA CCAAACTTAC AGAAGAATGG 
TCCAGAGAGC AGTCCTCAGA AAGCAGTCAT GAGACCCCAG TGGAAGATCC AGCCCAGGGC 
TGTTCAGGAT GCATTTCTGA ACTGTCCCTT TCTGGGCCTA ATGCTTCAGT GCCTAGGGAT 
TTTCTGGAGA ATCAGGTAAC CATTCAGAGT CTCGAGCTGA AAGTAAAAGA GACATCAAAT 
GAGAATTTGA GATTACTTCA TGGCATAGAG GAGCGTGACC AAAAAGTTGA AAACTTGCTA 
AATGAAATCA AAGAGTTAGA CTCAAAATTC CATTTACTGG AAGTACAATT AACTACCAAG 
ATTGAAGCAT GTGTGGCATT GGAAAAAATA GTTGAGGAAC TTAAGAAAGA AAAATTAGAT 
TTAAATGAAA AATTGGAATC CTTTTCTTGT CATAACCAGA GAGAAGAAAG TTCAGGAGGT 
CTCACTTCCA ACTTAGAAAT GGTCACAAGC AAATTTCCAC ATGAAGGTAT TGAAGATGAT 
GTAGCCAAGG TGACTGACAA CTGGAGAGAG AAATGTCTCC AGGTGGAAAA TGAGCTACAG 
AGGATTCAAT CTGAGAAAGA TAGCATGGAG CATCATGCCC TCTCTGCTGA AGCCAGCTTA 
GAGGCAGTTC AAACAGAGAA ACTATATTTA GAAAAAGACA ATGAAAATAA ACAAACAGTT 
ATAACCTGTC TTGAGGAAGG ACTCTCAGTG GTCACAAGTG AGAGAGATCA GCTTCGTGGA 
GAGTTAGATA CTTTGTCAAA AGAAAATCAA GAGCTAGATC AGATGTCTGA AAAGATGAAG 
GAGAAAATAC GGGAGCTTGA ATCTCATCAA AGTGAGTGTC TGCATCTCCA AGAGTGGCTT 
CAGAGTTTGG AAAAGGACTC ACAGGCACTG TCTCTGGTAA GAAGTGAGCT GGAAAACCAA 
ATTGAACAAC TGAATAAAGA GAAAGACTCA CTTGTATGGG AATCCGAAAG CCTGCAGACC 
AAACTGAGCG AATCGGAGCA TGAAAAGCTG GCCATCACCA AGGCTTTGGA GGCTGCCCTG 
ATGGAGAAGG GTGAGGTCGC GGTGAGGCTG AGCTCCACGC AAGAGGAAGT GCACCAGTTG 
AGAAAAGGCA TTGAGAAACT CAGGGTCCGC ATTGAGGCTG ATGAAAAGAA GCAGCTCCAT 
GTCTCAGAGA AACTGAAAGA AAGCGAGCGG AGGAATGACT CGCTTCAGGA CAAGGTTGAG 
ACCCTTGAAA GGGAGTTGCA GATGGCAGAA GAAAACCAAG AGCTGGTGAT TCTTGATGCT 
GAGAATTGCA AAGCAGAGGT GGAAACCCTA AAAACACAAA TAGAATTGAT GACTGAACGC 
CTAAAAGATT TAGAATTAGA CCTTGTCACA ATACGGTCTG AAAAAGAAAA TCTTTTGAAA 
CAGCTACAAG AGAAACAAGG TCAGGCGTCT GAATTAGATA CGCTACTCTC TTCATTGAAA 
AACCTATTAG AAGAAAAGGA GCGAGAGAAA ATACAGACGA AAGAAGAAGC TAAAAGTGCA 
GTGGAGATGC TGCAAACACA GTTGAGAGAG CTAACTGAGG AAATAGCAGC CTTATGTGAT 
GACCAGGAGA CATGGAAGGT CGAAGAACAG AGTCTGGACT CTCCTGCCCA GGAGGTACAA 
CAGCTGAGAA ATAACATTGA AAAGCTGAAA GTCCACTTGG ACATTGATAA AAAGAAGCAG 
CTCCAAATCC TAGAAAAACT GAAGGAAAGT GAGCATCAGG CCGATTTCCT TAAGGATACA 
GTTGAGAATC TTGAAAGAGA ACTCAAGCTA TCAGGAGAAA ACCAAGAGCA TGTGACTCTT 
GAGGCTGAGA AGTCCAAAGC AGAAGTAGAG ACCCTGAAAG CAACAGTGGA GAAGATGGAC 
CAAAATCTGA GAGGTTTGCA ATTAGATCTT GTCAATATAA GATCAGAAAA AGAAGATCTG 
ACTAAAGAAT TACAGAAAGA GCAGAGTCGA GTATCTGAAT TAGAAACGTT AAATTCTTCA 
TTTGAAAACC TATTGCGGGA AAAAGAGCAA GAAAAAGTAC AGATGAAGGA AGAATCTAAA 
GCTGCAGTGG AGATGCTGCA AACACAGCTG AAAGAGCTAA GTGAAGAAGT AGCAATCTTG 
TGTGTTGACC AGGAGACCTG GAAGGTTGAA GAACAGAGTC TGGACTCCCC ACTTGAGGAG 
ATACAACAGC TGAGAAATAA CATTGGAAAG CTGAAAGTCT GCCTAGATGC TGATAAAAAG 
CAGCAGCTCC ATATCTTAGA AAAACTGAAG GAAAGTGAGC ATCAGGCCGA TTTCCTTAAG 
GATACAGTTG AGAACCTTGA AAGAGAACTC AAGCTATCAG GAGAAAACCA AGAGCATGTG 
ACTCTTGAGG CTGAGAAGTC CAAAGCAGAA GTAGAGACCC TGAAAGCAAC AGTGGAGGAG 
ATGGACCAAA ATCTGAGAGG TTTGCAATTA GATCTTGTCA ATATAAGATC AGACAAAGAA 
GATCTGACTA AAGAATTACA GAAAGAGCAG AGTCGAGTGT CTGAATTAGA AACGTTAAAT 
TCTTCATTTG AAAACCTATT GCGGGAAAAA GAGCAAGAAA AAGTACAGAT GAAGGAAGAA 
TCTAAAGCTG CAGTGGAGAT GCTGCAAAAA GAATTAGAAG AGCTAAACGA GAAAATGGCA 
GCCTTGGGTA ATGACCAAGA GACCCGGAAG GTCAAAGAAC AGAGCCTGAG TAGTCAAGTA 
GATTCCCTTG AACATGAGAA GGCTCAGTTG CTGCAGGACC TTGATGATGC CAAAAGTAAT 
TACATGATTT TGCAGTCTTC TGTGAATGAC CTCATTCAAG AAGTTGAAGA TGGCAAACAG 
AAACTAGAGA AAAAGGATGA AGAAATCAGT ATACTAAAAA GTCAGACTCG GGACCAAGAG 
CAGCTTGTCT CTAAACTGTC CCAGATGGAA GGAGAACAGC AACTTTGGAA GAAGCAAAAG 
GCAGACCTGG AAAATCTGAT GGTGGAATTG GAGCAGAAGA TTCAGGTGCT GCAATCCAAA 
AACGATGCTT TGCAGGACAC CTTAGAAGCT CTGCAGAATT CTTCCAGGAA TCTGGAGAAA 
GAGCTTGAAT TGACAAAATT GGAAAAAATG TCCTTTGTTG AAAAAGTAAA CACAATGACT 
GTGAAGGAAA CTGAGCTTCA GAAGGAAATA CATGCAGTGA TACAAAAAAC GGTGGCACTG 
AAAGAAGAAT TTAGTGGGGA GAAAAACAGG CTAATGGAAG AATTAAATTT AATGTTGGAA 
GAAGTGAAGA GCAGCAAAGG TCAACTGAAG GAGCTCATGC TAGAAAATAG TGAATTGAAG 
AAGAGTTTGG ATTGTGTACA CAAAGACCGT ATGGAAGAAC AAGGGAAAAT GAGAGGAGAA 
ATAGCTGAAT ATCAGCTACG GCTTCAGGAA GCTGAAAACA AACATCAGGC TTTGCTTCTA 
GATACAAACA AACAGCATGA AATGGAAATC CAGACATACC GAGAGAAACT GACTTCTAAG 
GAGGAATGTC TCAGCTCACA GAAGGTGGAG ATAGACCTCT TAAAGTCCAG TAAAGAAGAA 
CTCAATAATT CTTTGAAAGC GACCACTGAG ATTTTAGAAG AATTGAAGAA AACCAAGATG 
GAAAATCTAA AACATGCAGA TAAATTGAAG AAGGAAAACG ATCGTGCCCA GAGTAAAATA 
AAGTTGTTGG TGAAATCCTG TAAACAGCTG GAAGAGGAAA AGGTGATGTT GCAGAAAGAA 
CTATCTCATC TTGAAGCTGC ACAGGAGAAA CAGAGAGCAG ATACCGTCGT GGATGCTAAT 
GTCGACGAAT TAATAACTGA AATGAAAGAA CTGAAAGAAA CCCTTGAAGA AAAAACCAAG 
GAGGCAGACG AATACCTGGA TAAATACTGT TCCCTGCTCA TAAGCCATGA GAAGTTAGAG 
AAAGCCAAGG AGATGTTAGA AACACAAGTT GCTCGTCTGA GTTCACAACA ATCTAAACTG 
AATCTTCGGA GATCTCCTTT GGTGAATTCG CTTGCTCCAG GACCATCCCC AGTCCCTTCT 
GCCACTGAGA AGAAGTTACC ATCCGGCCAA AATAAATCTT CAGGCAAAAG GCAAAGGTCC 
AGTGGGATTC GGGAGGATGG CGGAGAAACC ACGCCTTCTA CACCAGAGAC ATTTTCTAAA 
AAAAGCAGGA AAGCCATCAG AAGTGGTACT CACCTTGCAG AGGATGCAGA AGATGCTGAA 
TTTGAGCCAG AGGGGCTTCC AGAAGTTGTA AAGAAAGGGT TTGCCGACAT CCCAACCGGA 
AAGACTAGCC CCTACATCCT GCGGAGGACA ACCATGGCAA CCAGGACCAG CCCCCGCCTT 
GCTGCCCAGA AGTTAGCAGC ATCCCCGCCA AGTCTCGACA AAGAAAATCT CACCGAGACC 
TCCAAACCAA CAGCTGGTGG CAGCAGATCA CAAAAGGTCA AGGTTTCTCA ACAGAGCCCA 
GTGGATTCAG GCACTGCCTT CCGAGATCCC ATTGGCAGAT CTCTCTCCAT CAGTAATCTT 
CCCGAGAGAA GTCCAGTGGG CAGCCCCAGG GAGGGCCTGA GAGCCAAGCG AGGCCGCCAC 
GCCCCCAGCC CAGAATCCAG CCTGGAGTCC AAGGGCAGCG AGAACTGTAG GGTCCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CENPF ( XM_005217369.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005217426.1 Bos taurus centromere protein F (CENPF), transcript variant X1, mRNA.

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CloneID	OBa27521
Clone ID Related Accession (Same CDS sequence)	XM_005217369.4 , XM_005217369.3
Accession Version	XM_005217369.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	centromere protein F isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104441.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005217369.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGGG CTTTGGAAGA ATGGAAGGAA GGCTTGCCTA CAAGAGCTCT TCAGAAGATT 
CAAGAGCTTG AAGGCCAGCT CGACAAACTG AAGAAAGAAA GGCAGCAACG GCAGTTTCAG 
CTTGAGACCC TGGAGGCTGC GTTGCAGAAG CAAAAACAGA AGGTTGAGAA TGAAAAAACT 
GAGGGTGCAA ACCTGAAAAG GGAGAACCAA AGCTTGATGG AAATATGTGA AAATCTGGAG 
AAGACTAAGC AGAAGATTTC TCATGAACTT CAAGTCAAGG AGTCACAAGT GAATTTCCAA 
GAAGGTCAAC TGAACTCAAG CAAAAAACAA ATAGAAAAGC TGGAACAGGA ACTTAAAAGG 
TGTAAATCTG AACTTGAAAG AAGCCAACAA GCTGCACAGT CTGCGGATGT CTCTCTGAAT 
TCATGCCTTA CACCACAAAA AATCTTTGCA ACTCCGCTCA CACCAAGCCA GTATTATAGC 
GGTTCCAAAT ATGAAGATCT AAAAGAAAAA TATAATAAAG AAGTTGAAGA ACGAAAAAGA 
TTAGAGGCAG AGGTAAAAGC CTTGCAGGCA AAGAAAGCAA GCCAGACTAT TCCCCAAAGC 
ACCATGAATC ACCGGGACAT CGCCCGACAT CAGGCTTCTT CATCTGTGTT CTCATGGCAA 
CAAGAAAAAA CCCCAAGCCG TCTTTCATCT AACACTCTGA AAACTCCAGT TAGGAGAGAT 
TTCTCTGCAT CTCATTTTTC TGGGGAACAA GAAGTGACTC CGAGTAGATC AACTTTGCAA 
ATAGGGAAAA CAGATGCTAA TAGCAGTTTC TGTGATAATT CTAGCAATTC TCATCTTTTG 
GACCAGTTAA AAGCACAGAA CCAAGAACTA AGGAGCAAGA TGAGTGAGCT GGAACTACGT 
CTGCAAGGAC AAGAAAAAGA AATGAAAGGC CAAGTGAATA AACTTCAAGA ACTCCAACTG 
CAACTGGAGA AAGCAAAAGT GGAATTAAAT GAAAAAGAGA AAGTTTTGAA CAAAAACCGG 
GATGAACTAG TGAGAACAAC TTCACAGTAT GACCAGGCAT CAGCCAAGTG TACTGCTTTG 
GAACAAAAAT TGAAAAAATT GACTGAAGAT TTGAGTTGTC AGCGTCAAAA TGCAGAAAGT 
GCCAGATGTT CTCTGGAACA GAAAATCAAG GAGAAAGAAA AAGAGTTCCA AGAGGAGCTC 
TCCCGTCAAC AGCGTTCCTT CCAAACCCTG GACCAGGAGT GCACTCAGAT GAAAGCCAGG 
CTCACCCAGG AGCTACAGCA AGCTAAGAAC ACATACAACA TCCTACAGGC TGAACTGGAT 
AAAGTCACAT CTGTAAAGCA ACAGATAGAA AAAAAATTGG AAGAGTTTAA GCAAAATTTC 
AGCAAAACTG AACAGGCCTT ACAGGCAAGT CAGACCAAGG AAAATGAGCT GAGGAGAAGC 
AGTGAGGAAA TGAAGAGGGA AAACAGCCTC CTCAAGAGTC AGTCTGAGCA AAGGGCCCGA 
GAAGTCTGCC ACCTGGAGGA AGAACTGAAG AAGGCCAAGC AGTGTTTGAG TCAGAGCCAG 
AATTTTGCAG AAGAAATGAA GGCTAAGAAT ACCTCTCAGG AAACCATGTT AAGAGATCTT 
CAAGAAAAGA TAAATCAGCA AGAGAATTCC TTGACTTTAG AGAAACTGAA GCTTGCCTTG 
GCTGACCTGG AAAAGCAGCG AGATTGTTCT CAAGACCTTT TGAAGAAAAG GGAACATCAC 
ATTGAACAAC TGAACGAAAA GTTAAGCAAA ACAGAGAGAG AATCTGAAGC ATTGTTGAGT 
GCTTTGGAAT TAAAGAAGAA AGAATATGAA GAATTAAAAG AAGAGAAAAC TCTGTTTTCT 
CGCTGGAAAA GTGAAAACGA ACAACTTTTA AATCAGATGG AATCAGAAAA AGAAAGCTTG 
CAGAGTAAAG TTAATCACTT GGAAACTTGT CTTAAGACAC AACAAATAAA AAGTCATGAA 
TATAATGAGA GGGTAAGAAC TCTGGAGATG GAAAGAGAAA ATCTAAACGT GGAGATCAGG 
AACCTTCAAA ATGTGATTGA CAGCAAAACC GCAGAGGCAG AGACACAGAA ACGAGCTTAT 
GGAGAACTGC AACAGAAAGC TGAGTTCTCA GATCAGAAAC ACGAGAAGGA GATAGAAAAT 
ATGTGTTTGA AAATCTCTCA CCTCACTGGG CAAGTGGAAG ACCTAGAACA TAAGCTTCAG 
TTACTGTCAA GTGAAATCAT GGACAAAGAC CAGCGTTACC AAGACTTGCA TGCTGAATCT 
GAGAGCCTCA GGGATCTGCT AAAATCCAGA GATTCCTCTG CGATGACAAT TGAGCCTCAT 
CAGAGAAGTC GTTTAGCTTT TGAACAGCAG TCTGCACTGA ACAATTCCTT TGCAAATATA 
ATCGGAGAGC AAGAAAGTGT GCCTTCAGAA AGGAGCAGAT GCCATGTAGC AACAGACCAA 
AGTCCGAAAA GTTCATCCAT GTTGCAAAAT AGAGTTGTTT CTCTTGAATT TTCCTTAGAG 
TCGCAAAAGC AGATGAACTC AGATCTGCAG AAGCAGTGTG AAGAGTTGGT GCAAATCAAA 
GGAGAAATTG AAGAAAACCT CATGAAAGCA GAACAGATGC ATCAAAGTTT TGTGGCTGAA 
ACAAGCCAAC GCATTAGTAA GTTACAAGAA GACACTTCTG TTCATCAGAA TGTTGTTGCT 
GAAACTTTAG CTGCCCTGGA GAGCAAGGAA AGAGAGTTGC AACTTTTGAA TGAAAAATTA 
GAAACTGAGC AGGCAGAGAC TGGAGAGTTA AAAAAGAACA ACCATTTACT TCAGGAATCT 
CTAAAGGAGC TACAGTTTTT GTCTGAAACC CTGAGCTTGG AGAAAAAGGA ACTGAATTCC 
ATCATTTCTC TAAATAAAAA GGACATTGAA GAGTTGACCC AAGAGAATGG GACTCTCAAG 
GAAATTAATG CCACCTTAAC TCAAGAGAAG ATGAACTTGC TCCAAAAAAC CGAGAGTTTT 
TCGAACTGTA TAGATGAAAG AGACAGAAGC ATTTCAGAGT TATCTAATCA GTACAAACAA 
GAAAGGCTTA CTTTACTACA AAGATGTGAA GAAACAGGAA ATGCATTTCA GGATCTTAGT 
GAAAAATATA AAGCGGTTCA GGAAAAGAAC TCTAAATTAG AATGCTTGCT GAGTGAGTGC 
ACGGGTGTTT GTGAAGACAG AAAAAATGAA TTAGGACAGC TAAAGGAAAC ATTTGCAAGG 
GAACACCAAG CATTTGTATC ACAGTTAGCA TTAGCTGAAG AAAGAAACCA GAATTTAATT 
GTGGAGTTGG AGACAGTGCA GCAAGCCCTG CAGTCTGAGA TTACAGATAT CCAAAACAGC 
TCCAAGAGGG AGACTGATGG TTTAAAGCAA GAAATCATGA ATTTAAAGGA AGAACAAAAT 
AAGATGCAAC AGGAAGTTAG TGCCTTATTA CAAGAGAATG AACACCTGAT GGAATTAATG 
AAGACGAAAC ATGAGCATCA ATGTCTAGAA TTGGAACCAA TTCAAGACTC TGAGAAAGAA 
GAGAGGGAGA TAAATACATG TCACCTTCAA CTTCCGATGG ATCTTGATGT TAAAGATACT 
TCTCCAGACA CTTGTAATGC ACAGCTGGTG CAAGTAGAAT CTAAAGTCAG AAACATGGAA 
TTAAAACTTC AGAAGAGTGA GAAGGAAGAG TGTCTACAGT ATGAGTTACA AACTAGAGAA 
TTAGAAACTG AAGATTTGCA ACAAGATCCT CAGTCACAAG ATATTAGTGG CCTTGGAGAC 
TCTGAAATAG ATCCAGAAGA AAAATATATT TCAGTGCTTC AGGAGTTGTC AACAAGTCAG 
CAGGACAATG CCCACCTGCA GTGTTCTCTA CAGACAGCCA TGAACAAGCT GAATGAGTTA 
GAGAAAATGT GTGAAGCCCT GCAGGTTGAA AAGTCTGAAC TCATATCTGA GCTGAATGAC 
TCAAGATCAG AATGTATCAC AGCAACTAGT AAAATGGCAG AAGAGGTAGG GAAACTAGTA 
AATGAAGTTG AAACATTAAA TGACGAAAAT GCACTTCTCC AAGGCGAGTT AGTGGAAGAA 
ACACCAGAAG GTGAATTTGG TGAACAACAA GATGAGCAGA CATCTGTGAA CTTAAATCCT 
TTGGATGACA GTAATTTCTA TGAGCATTTG ACCATGTCAA GCAAAGAGGT TCAAATGCAC 
TTTGCTGAAT TACAGGAGAA ATTTTCATCT TTACAGAGTG AGCACAAAAT TTTACACGAA 
CAGCACTGTC TGATGAGCTC TAAGATGTCA GAGCTGCAGT CCTACGTCGA CACGTTAAAG 
GCTGAAAATT CAGTCTTGTC GATGAGTCTG AGAAGCTCTC AAGGTGACTT GGTAAAGGAG 
GTGCCACCAG GACCTGGGGA GGAGCATTTA CTGTCCTTGT CATTTTCTTG TGTGACTGAC 
AGCCCTGGTA AGGCAAGTTT GGGAGAGTCC TCTTTTTACA AAGATCTTCT AGAGCACACA 
ACAGAAACGT CCCTTTTGAA TAATCTAGAA GGGACTGTTT CGGCAAACCA GTCCAATGTA 
GAGGAAGTCT CTTGTAGTAG TCTGGAGGAG GAGAATCTGA CCGAGAAAGA GATCCCCTCT 
GTCCCACTGA GGAGCGCTCG AGAACTTGAG ACCCTCTGTC AGACGTACCT CGAGTCCCTC 
AAGCAGCTGG AAGAGGAAAT CGAAAGTCAA GGGATTACGA AAAACAAGGA GATCAAAGAG 
CTCAAGGAGC TACTGAGTTC TTCGAGGGAG GAGCTTGACG ACCTCAGGAA GCAGTATTTG 
TCAGAAAATG AACAGTGGCA GCAGAAGCTG ACAGATGTGA CAGCGGAGAT GGAGTCCAAG 
TTGGCGGCTG AAAAGAGACA CGCAGAGCAC CTTACACTTG AGCTTGAGGT GGCGCGGCTT 
CAGCTGCAGG GTCTGGACTT AAGCTCTCGG TCTCTGCTTG GCACCGACAT AGAAGATGCT 
ATTCTGGGTG GAAACGATAG CTGTGACATA AGAGAATCAG AAGAATATAT TTCAGAAACT 
AAAGAGAGGA CCCCAAAGCA TGAGATTCAC CAGATTTGTG AAAAAGATGT TCAGCAGGAC 
CTCAGTCTAG AGATGGAGAA AATAACAAAG ACTGGTGCAA CCAAACTTAC AGAAGAATGG 
TCCAGAGAGC AGTCCTCAGA AAGCAGTCAT GAGACCCCAG TGGAAGATCC AGCCCAGGGC 
TGTTCAGGAT GCATTTCTGA ACTGTCCCTT TCTGGGCCTA ATGCTTCAGT GCCTAGGGAT 
TTTCTGGAGA ATCAGGTAAC CATTCAGAGT CTCGAGCTGA AAGTAAAAGA GACATCAAAT 
GAGAATTTGA GATTACTTCA TGGCATAGAG GAGCGTGACC AAAAAGTTGA AAACTTGCTA 
AATGAAATCA AAGAGTTAGA CTCAAAATTC CATTTACTGG AAGTACAATT AACTACCAAG 
ATTGAAGCAT GTGTGGCATT GGAAAAAATA GTTGAGGAAC TTAAGAAAGA AAAATTAGAT 
TTAAATGAAA AATTGGAATC CTTTTCTTGT CATAACCAGA GAGAAGAAAG TTCAGGAGGT 
CTCACTTCCA ACTTAGAAAT GGTCACAAGC AAATTTCCAC ATGAAGGTAT TGAAGATGAT 
GTAGCCAAGG TGACTGACAA CTGGAGAGAG AAATGTCTCC AGGTGGAAAA TGAGCTACAG 
AGGATTCAAT CTGAGAAAGA TAGCATGGAG CATCATGCCC TCTCTGCTGA AGCCAGCTTA 
GAGGCAGTTC AAACAGAGAA ACTATATTTA GAAAAAGACA ATGAAAATAA ACAAACAGTT 
ATAACCTGTC TTGAGGAAGG ACTCTCAGTG GTCACAAGTG AGAGAGATCA GCTTCGTGGA 
GAGTTAGATA CTTTGTCAAA AGAAAATCAA GAGCTAGATC AGATGTCTGA AAAGATGAAG 
GAGAAAATAC GGGAGCTTGA ATCTCATCAA AGTGAGTGTC TGCATCTCCA AGAGTGGCTT 
CAGAGTTTGG AAAAGGACTC ACAGGCACTG TCTCTGGTAA GAAGTGAGCT GGAAAACCAA 
ATTGAACAAC TGAATAAAGA GAAAGACTCA CTTGTATGGG AATCCGAAAG CCTGCAGACC 
AAACTGAGCG AATCGGAGCA TGAAAAGCTG GCCATCACCA AGGCTTTGGA GGCTGCCCTG 
ATGGAGAAGG GTGAGGTCGC GGTGAGGCTG AGCTCCACGC AAGAGGAAGT GCACCAGTTG 
AGAAAAGGCA TTGAGAAACT CAGGGTCCGC ATTGAGGCTG ATGAAAAGAA GCAGCTCCAT 
GTCTCAGAGA AACTGAAAGA AAGCGAGCGG AGGAATGACT CGCTTCAGGA CAAGGTTGAG 
ACCCTTGAAA GGGAGTTGCA GATGGCAGAA GAAAACCAAG AGCTGGTGAT TCTTGATGCT 
GAGAATTGCA AAGCAGAGGT GGAAACCCTA AAAACACAAA TAGAATTGAT GACTGAACGC 
CTAAAAGATT TAGAATTAGA CCTTGTCACA ATACGGTCTG AAAAAGAAAA TCTTTTGAAA 
CAGCTACAAG AGAAACAAGG TCAGGCGTCT GAATTAGATA CGCTACTCTC TTCATTGAAA 
AACCTATTAG AAGAAAAGGA GCGAGAGAAA ATACAGACGA AAGAAGAAGC TAAAAGTGCA 
GTGGAGATGC TGCAAACACA GTTGAGAGAG CTAACTGAGG AAATAGCAGC CTTATGTGAT 
GACCAGGAGA CATGGAAGGT CGAAGAACAG AGTCTGGACT CTCCTGCCCA GGAGGTACAA 
CAGCTGAGAA ATAACATTGA AAAGCTGAAA GTCCACTTGG ACATTGATAA AAAGAAGCAG 
CTCCAAATCC TAGAAAAACT GAAGGAAAGT GAGCATCAGG CCGATTTCCT TAAGGATACA 
GTTGAGAATC TTGAAAGAGA ACTCAAGCTA TCAGGAGAAA ACCAAGAGCA TGTGACTCTT 
GAGGCTGAGA AGTCCAAAGC AGAAGTAGAG ACCCTGAAAG CAACAGTGGA GAAGATGGAC 
CAAAATCTGA GAGGTTTGCA ATTAGATCTT GTCAATATAA GATCAGAAAA AGAAGATCTG 
ACTAAAGAAT TACAGAAAGA GCAGAGTCGA GTATCTGAAT TAGAAACGTT AAATTCTTCA 
TTTGAAAACC TATTGCGGGA AAAAGAGCAA GAAAAAGTAC AGATGAAGGA AGAATCTAAA 
GCTGCAGTGG AGATGCTGCA AACACAGCTG AAAGAGCTAA GTGAAGAAGT AGCAATCTTG 
TGTGTTGACC AGGAGACCTG GAAGGTTGAA GAACAGAGTC TGGACTCCCC ACTTGAGGAG 
ATACAACAGC TGAGAAATAA CATTGGAAAG CTGAAAGTCT GCCTAGATGC TGATAAAAAG 
CAGCAGCTCC ATATCTTAGA AAAACTGAAG GAAAGTGAGC ATCAGGCCGA TTTCCTTAAG 
GATACAGTTG AGAACCTTGA AAGAGAACTC AAGCTATCAG GAGAAAACCA AGAGCATGTG 
ACTCTTGAGG CTGAGAAGTC CAAAGCAGAA GTAGAGACCC TGAAAGCAAC AGTGGAGGAG 
ATGGACCAAA ATCTGAGAGG TTTGCAATTA GATCTTGTCA ATATAAGATC AGACAAAGAA 
GATCTGACTA AAGAATTACA GAAAGAGCAG AGTCGAGTGT CTGAATTAGA AACGTTAAAT 
TCTTCATTTG AAAACCTATT GCGGGAAAAA GAGCAAGAAA AAGTACAGAT GAAGGAAGAA 
TCTAAAGCTG CAGTGGAGAT GCTGCAAAAA GAATTAGAAG AGCTAAACGA GAAAATGGCA 
GCCTTGGGTA ATGACCAAGA GACCCGGAAG GTCAAAGAAC AGAGCCTGAG TAGTCAAGTA 
GATTCCCTTG AACATGAGAA GGCTCAGTTG CTGCAGGACC TTGATGATGC CAAAAGTAAT 
TACATGATTT TGCAGTCTTC TGTGAATGAC CTCATTCAAG AAGTTGAAGA TGGCAAACAG 
AAACTAGAGA AAAAGGATGA AGAAATCAGT ATACTAAAAA GTCAGACTCG GGACCAAGAG 
CAGCTTGTCT CTAAACTGTC CCAGATGGAA GGAGAACAGC AACTTTGGAA GAAGCAAAAG 
GCAGACCTGG AAAATCTGAT GGTGGAATTG GAGCAGAAGA TTCAGGTGCT GCAATCCAAA 
AACGATGCTT TGCAGGACAC CTTAGAAGCT CTGCAGAATT CTTCCAGGAA TCTGGAGAAA 
GAGCTTGAAT TGACAAAATT GGAAAAAATG TCCTTTGTTG AAAAAGTAAA CACAATGACT 
GTGAAGGAAA CTGAGCTTCA GAAGGAAATA CATGCAGTGA TACAAAAAAC GGTGGCACTG 
AAAGAAGAAT TTAGTGGGGA GAAAAACAGG CTAATGGAAG AATTAAATTT AATGTTGGAA 
GAAGTGAAGA GCAGCAAAGG TCAACTGAAG GAGCTCATGC TAGAAAATAG TGAATTGAAG 
AAGAGTTTGG ATTGTGTACA CAAAGACCGT ATGGAAGAAC AAGGGAAAAT GAGAGGAGAA 
ATAGCTGAAT ATCAGCTACG GCTTCAGGAA GCTGAAAACA AACATCAGGC TTTGCTTCTA 
GATACAAACA AACAGCATGA AATGGAAATC CAGACATACC GAGAGAAACT GACTTCTAAG 
GAGGAATGTC TCAGCTCACA GAAGGTGGAG ATAGACCTCT TAAAGTCCAG TAAAGAAGAA 
CTCAATAATT CTTTGAAAGC GACCACTGAG ATTTTAGAAG AATTGAAGAA AACCAAGATG 
GAAAATCTAA AACATGCAGA TAAATTGAAG AAGGAAAACG ATCGTGCCCA GAGTAAAATA 
AAGTTGTTGG TGAAATCCTG TAAACAGCTG GAAGAGGAAA AGGTGATGTT GCAGAAAGAA 
CTATCTCATC TTGAAGCTGC ACAGGAGAAA CAGAGAGCAG ATACCGTCGT GGATGCTAAT 
GTCGACGAAT TAATAACTGA AATGAAAGAA CTGAAAGAAA CCCTTGAAGA AAAAACCAAG 
GAGGCAGACG AATACCTGGA TAAATACTGT TCCCTGCTCA TAAGCCATGA GAAGTTAGAG 
AAAGCCAAGG AGATGTTAGA AACACAAGTT GCTCGTCTGA GTTCACAACA ATCTAAACTG 
AATCTTCGGA GATCTCCTTT GGTGAATTCG CTTGCTCCAG GACCATCCCC AGTCCCTTCT 
GCCACTGAGA AGAAGTTACC ATCCGGCCAA AATAAATCTT CAGGCAAAAG GCAAAGGTCC 
AGTGGGATTC GGGAGGATGG CGGAGAAACC ACGCCTTCTA CACCAGAGAC ATTTTCTAAA 
AAAAGCAGGA AAGCCATCAG AAGTGGTACT CACCTTGCAG AGGATGCAGA AGATGCTGAA 
TTTGAGCCAG AGGGGCTTCC AGAAGTTGTA AAGAAAGGGT TTGCCGACAT CCCAACCGGA 
AAGACTAGCC CCTACATCCT GCGGAGGACA ACCATGGCAA CCAGGACCAG CCCCCGCCTT 
GCTGCCCAGA AGTTAGCAGC ATCCCCGCCA AGTCTCGACA AAGAAAATCT CACCGAGACC 
TCCAAACCAA CAGCTGGTGG CAGCAGATCA CAAAAGGTCA AGGTTTCTCA ACAGAGCCCA 
GTGGATTCAG GCACTGCCTT CCGAGATCCC ATTGGCAGAT CTCTCTCCAT CAGTAATCTT 
CCCGAGAGAA GTCCAGTGGG CAGCCCCAGG GAGGGCCTGA GAGCCAAGCG AGGCCGCCAC 
GCCCCCAGCC CAGAATCCAG CCTGGAGTCC AAGGGCAGCG AGAACTGTAG GGTCCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005217426.1
CDS	241..10020
Translation	MSWALEEWKEGLPTRALQKIQELEGQLDKLKKERQQRQFQLETLEAALQKQKQKVENEKTEGANLKRENQSLMEICENLEKTKQKISHELQVKESQVNFQEGQLNSSKKQIEKLEQELKRCKSELERSQQAAQSADVSLNSCLTPQKIFATPLTPSQYYSGSKYEDLKEKYNKEVEERKRLEAEVKALQAKKASQTIPQSTMNHRDIARHQASSSVFSWQQEKTPSRLSSNTLKTPVRRDFSASHFSGEQEVTPSRSTLQIGKTDANSSFCDNSSNSHLLDQLKAQNQELRSKMSELELRLQGQEKEMKGQVNKLQELQLQLEKAKVELNEKEKVLNKNRDELVRTTSQYDQASAKCTALEQKLKKLTEDLSCQRQNAESARCSLEQKIKEKEKEFQEELSRQQRSFQTLDQECTQMKARLTQELQQAKNTYNILQAELDKVTSVKQQIEKKLEEFKQNFSKTEQALQASQTKENELRRSSEEMKRENSLLKSQSEQRAREVCHLEEELKKAKQCLSQSQNFAEEMKAKNTSQETMLRDLQEKINQQENSLTLEKLKLALADLEKQRDCSQDLLKKREHHIEQLNEKLSKTERESEALLSALELKKKEYEELKEEKTLFSRWKSENEQLLNQMESEKESLQSKVNHLETCLKTQQIKSHEYNERVRTLEMERENLNVEIRNLQNVIDSKTAEAETQKRAYGELQQKAEFSDQKHEKEIENMCLKISHLTGQVEDLEHKLQLLSSEIMDKDQRYQDLHAESESLRDLLKSRDSSAMTIEPHQRSRLAFEQQSALNNSFANIIGEQESVPSERSRCHVATDQSPKSSSMLQNRVVSLEFSLESQKQMNSDLQKQCEELVQIKGEIEENLMKAEQMHQSFVAETSQRISKLQEDTSVHQNVVAETLAALESKERELQLLNEKLETEQAETGELKKNNHLLQESLKELQFLSETLSLEKKELNSIISLNKKDIEELTQENGTLKEINATLTQEKMNLLQKTESFSNCIDERDRSISELSNQYKQERLTLLQRCEETGNAFQDLSEKYKAVQEKNSKLECLLSECTGVCEDRKNELGQLKETFAREHQAFVSQLALAEERNQNLIVELETVQQALQSEITDIQNSSKRETDGLKQEIMNLKEEQNKMQQEVSALLQENEHLMELMKTKHEHQCLELEPIQDSEKEEREINTCHLQLPMDLDVKDTSPDTCNAQLVQVESKVRNMELKLQKSEKEECLQYELQTRELETEDLQQDPQSQDISGLGDSEIDPEEKYISVLQELSTSQQDNAHLQCSLQTAMNKLNELEKMCEALQVEKSELISELNDSRSECITATSKMAEEVGKLVNEVETLNDENALLQGELVEETPEGEFGEQQDEQTSVNLNPLDDSNFYEHLTMSSKEVQMHFAELQEKFSSLQSEHKILHEQHCLMSSKMSELQSYVDTLKAENSVLSMSLRSSQGDLVKEVPPGPGEEHLLSLSFSCVTDSPGKASLGESSFYKDLLEHTTETSLLNNLEGTVSANQSNVEEVSCSSLEEENLTEKEIPSVPLRSARELETLCQTYLESLKQLEEEIESQGITKNKEIKELKELLSSSREELDDLRKQYLSENEQWQQKLTDVTAEMESKLAAEKRHAEHLTLELEVARLQLQGLDLSSRSLLGTDIEDAILGGNDSCDIRESEEYISETKERTPKHEIHQICEKDVQQDLSLEMEKITKTGATKLTEEWSREQSSESSHETPVEDPAQGCSGCISELSLSGPNASVPRDFLENQVTIQSLELKVKETSNENLRLLHGIEERDQKVENLLNEIKELDSKFHLLEVQLTTKIEACVALEKIVEELKKEKLDLNEKLESFSCHNQREESSGGLTSNLEMVTSKFPHEGIEDDVAKVTDNWREKCLQVENELQRIQSEKDSMEHHALSAEASLEAVQTEKLYLEKDNENKQTVITCLEEGLSVVTSERDQLRGELDTLSKENQELDQMSEKMKEKIRELESHQSECLHLQEWLQSLEKDSQALSLVRSELENQIEQLNKEKDSLVWESESLQTKLSESEHEKLAITKALEAALMEKGEVAVRLSSTQEEVHQLRKGIEKLRVRIEADEKKQLHVSEKLKESERRNDSLQDKVETLERELQMAEENQELVILDAENCKAEVETLKTQIELMTERLKDLELDLVTIRSEKENLLKQLQEKQGQASELDTLLSSLKNLLEEKEREKIQTKEEAKSAVEMLQTQLRELTEEIAALCDDQETWKVEEQSLDSPAQEVQQLRNNIEKLKVHLDIDKKKQLQILEKLKESEHQADFLKDTVENLERELKLSGENQEHVTLEAEKSKAEVETLKATVEKMDQNLRGLQLDLVNIRSEKEDLTKELQKEQSRVSELETLNSSFENLLREKEQEKVQMKEESKAAVEMLQTQLKELSEEVAILCVDQETWKVEEQSLDSPLEEIQQLRNNIGKLKVCLDADKKQQLHILEKLKESEHQADFLKDTVENLERELKLSGENQEHVTLEAEKSKAEVETLKATVEEMDQNLRGLQLDLVNIRSDKEDLTKELQKEQSRVSELETLNSSFENLLREKEQEKVQMKEESKAAVEMLQKELEELNEKMAALGNDQETRKVKEQSLSSQVDSLEHEKAQLLQDLDDAKSNYMILQSSVNDLIQEVEDGKQKLEKKDEEISILKSQTRDQEQLVSKLSQMEGEQQLWKKQKADLENLMVELEQKIQVLQSKNDALQDTLEALQNSSRNLEKELELTKLEKMSFVEKVNTMTVKETELQKEIHAVIQKTVALKEEFSGEKNRLMEELNLMLEEVKSSKGQLKELMLENSELKKSLDCVHKDRMEEQGKMRGEIAEYQLRLQEAENKHQALLLDTNKQHEMEIQTYREKLTSKEECLSSQKVEIDLLKSSKEELNNSLKATTEILEELKKTKMENLKHADKLKKENDRAQSKIKLLVKSCKQLEEEKVMLQKELSHLEAAQEKQRADTVVDANVDELITEMKELKETLEEKTKEADEYLDKYCSLLISHEKLEKAKEMLETQVARLSSQQSKLNLRRSPLVNSLAPGPSPVPSATEKKLPSGQNKSSGKRQRSSGIREDGGETTPSTPETFSKKSRKAIRSGTHLAEDAEDAEFEPEGLPEVVKKGFADIPTGKTSPYILRRTTMATRTSPRLAAQKLAASPPSLDKENLTETSKPTAGGSRSQKVKVSQQSPVDSGTAFRDPIGRSLSISNLPERSPVGSPREGLRAKRGRHAPSPESSLESKGSENCRVQ

Target ORF information:

RefSeq Version	XM_005217369.3
Organism	Bos taurus(cattle)
Definition	Bos taurus centromere protein F (CENPF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005217369.3

ORF Insert Sequence:

ATGAGCTGGG CTTTGGAAGA ATGGAAGGAA GGCTTGCCTA CAAGAGCTCT TCAGAAGATT 
CAAGAGCTTG AAGGCCAGCT CGACAAACTG AAGAAAGAAA GGCAGCAACG GCAGTTTCAG 
CTTGAGACCC TGGAGGCTGC GTTGCAGAAG CAAAAACAGA AGGTTGAGAA TGAAAAAACT 
GAGGGTGCAA ACCTGAAAAG GGAGAACCAA AGCTTGATGG AAATATGTGA AAATCTGGAG 
AAGACTAAGC AGAAGATTTC TCATGAACTT CAAGTCAAGG AGTCACAAGT GAATTTCCAA 
GAAGGTCAAC TGAACTCAAG CAAAAAACAA ATAGAAAAGC TGGAACAGGA ACTTAAAAGG 
TGTAAATCTG AACTTGAAAG AAGCCAACAA GCTGCACAGT CTGCGGATGT CTCTCTGAAT 
TCATGCCTTA CACCACAAAA AATCTTTGCA ACTCCGCTCA CACCAAGCCA GTATTATAGC 
GGTTCCAAAT ATGAAGATCT AAAAGAAAAA TATAATAAAG AAGTTGAAGA ACGAAAAAGA 
TTAGAGGCAG AGGTAAAAGC CTTGCAGGCA AAGAAAGCAA GCCAGACTAT TCCCCAAAGC 
ACCATGAATC ACCGGGACAT CGCCCGACAT CAGGCTTCTT CATCTGTGTT CTCATGGCAA 
CAAGAAAAAA CCCCAAGCCG TCTTTCATCT AACACTCTGA AAACTCCAGT TAGGAGAGAT 
TTCTCTGCAT CTCATTTTTC TGGGGAACAA GAAGTGACTC CGAGTAGATC AACTTTGCAA 
ATAGGGAAAA CAGATGCTAA TAGCAGTTTC TGTGATAATT CTAGCAATTC TCATCTTTTG 
GACCAGTTAA AAGCACAGAA CCAAGAACTA AGGAGCAAGA TGAGTGAGCT GGAACTACGT 
CTGCAAGGAC AAGAAAAAGA AATGAAAGGC CAAGTGAATA AACTTCAAGA ACTCCAACTG 
CAACTGGAGA AAGCAAAAGT GGAATTAAAT GAAAAAGAGA AAGTTTTGAA CAAAAACCGG 
GATGAACTAG TGAGAACAAC TTCACAGTAT GACCAGGCAT CAGCCAAGTG TACTGCTTTG 
GAACAAAAAT TGAAAAAATT GACTGAAGAT TTGAGTTGTC AGCGTCAAAA TGCAGAAAGT 
GCCAGATGTT CTCTGGAACA GAAAATCAAG GAGAAAGAAA AAGAGTTCCA AGAGGAGCTC 
TCCCGTCAAC AGCGTTCCTT CCAAACCCTG GACCAGGAGT GCACTCAGAT GAAAGCCAGG 
CTCACCCAGG AGCTACAGCA AGCTAAGAAC ACATACAACA TCCTACAGGC TGAACTGGAT 
AAAGTCACAT CTGTAAAGCA ACAGATAGAA AAAAAATTGG AAGAGTTTAA GCAAAATTTC 
AGCAAAACTG AACAGGCCTT ACAGGCAAGT CAGACCAAGG AAAATGAGCT GAGGAGAAGC 
AGTGAGGAAA TGAAGAGGGA AAACAGCCTC CTCAAGAGTC AGTCTGAGCA AAGGGCCCGA 
GAAGTCTGCC ACCTGGAGGA AGAACTGAAG AAGGCCAAGC AGTGTTTGAG TCAGAGCCAG 
AATTTTGCAG AAGAAATGAA GGCTAAGAAT ACCTCTCAGG AAACCATGTT AAGAGATCTT 
CAAGAAAAGA TAAATCAGCA AGAGAATTCC TTGACTTTAG AGAAACTGAA GCTTGCCTTG 
GCTGACCTGG AAAAGCAGCG AGATTGTTCT CAAGACCTTT TGAAGAAAAG GGAACATCAC 
ATTGAACAAC TGAACGAAAA GTTAAGCAAA ACAGAGAGAG AATCTGAAGC ATTGTTGAGT 
GCTTTGGAAT TAAAGAAGAA AGAATATGAA GAATTAAAAG AAGAGAAAAC TCTGTTTTCT 
CGCTGGAAAA GTGAAAACGA ACAACTTTTA AATCAGATGG AATCAGAAAA AGAAAGCTTG 
CAGAGTAAAG TTAATCACTT GGAAACTTGT CTTAAGACAC AACAAATAAA AAGTCATGAA 
TATAATGAGA GGGTAAGAAC TCTGGAGATG GAAAGAGAAA ATCTAAACGT GGAGATCAGG 
AACCTTCAAA ATGTGATTGA CAGCAAAACC GCAGAGGCAG AGACACAGAA ACGAGCTTAT 
GGAGAACTGC AACAGAAAGC TGAGTTCTCA GATCAGAAAC ACGAGAAGGA GATAGAAAAT 
ATGTGTTTGA AAATCTCTCA CCTCACTGGG CAAGTGGAAG ACCTAGAACA TAAGCTTCAG 
TTACTGTCAA GTGAAATCAT GGACAAAGAC CAGCGTTACC AAGACTTGCA TGCTGAATCT 
GAGAGCCTCA GGGATCTGCT AAAATCCAGA GATTCCTCTG CGATGACAAT TGAGCCTCAT 
CAGAGAAGTC GTTTAGCTTT TGAACAGCAG TCTGCACTGA ACAATTCCTT TGCAAATATA 
ATCGGAGAGC AAGAAAGTGT GCCTTCAGAA AGGAGCAGAT GCCATGTAGC AACAGACCAA 
AGTCCGAAAA GTTCATCCAT GTTGCAAAAT AGAGTTGTTT CTCTTGAATT TTCCTTAGAG 
TCGCAAAAGC AGATGAACTC AGATCTGCAG AAGCAGTGTG AAGAGTTGGT GCAAATCAAA 
GGAGAAATTG AAGAAAACCT CATGAAAGCA GAACAGATGC ATCAAAGTTT TGTGGCTGAA 
ACAAGCCAAC GCATTAGTAA GTTACAAGAA GACACTTCTG TTCATCAGAA TGTTGTTGCT 
GAAACTTTAG CTGCCCTGGA GAGCAAGGAA AGAGAGTTGC AACTTTTGAA TGAAAAATTA 
GAAACTGAGC AGGCAGAGAC TGGAGAGTTA AAAAAGAACA ACCATTTACT TCAGGAATCT 
CTAAAGGAGC TACAGTTTTT GTCTGAAACC CTGAGCTTGG AGAAAAAGGA ACTGAATTCC 
ATCATTTCTC TAAATAAAAA GGACATTGAA GAGTTGACCC AAGAGAATGG GACTCTCAAG 
GAAATTAATG CCACCTTAAC TCAAGAGAAG ATGAACTTGC TCCAAAAAAC CGAGAGTTTT 
TCGAACTGTA TAGATGAAAG AGACAGAAGC ATTTCAGAGT TATCTAATCA GTACAAACAA 
GAAAGGCTTA CTTTACTACA AAGATGTGAA GAAACAGGAA ATGCATTTCA GGATCTTAGT 
GAAAAATATA AAGCGGTTCA GGAAAAGAAC TCTAAATTAG AATGCTTGCT GAGTGAGTGC 
ACGGGTGTTT GTGAAGACAG AAAAAATGAA TTAGGACAGC TAAAGGAAAC ATTTGCAAGG 
GAACACCAAG CATTTGTATC ACAGTTAGCA TTAGCTGAAG AAAGAAACCA GAATTTAATT 
GTGGAGTTGG AGACAGTGCA GCAAGCCCTG CAGTCTGAGA TTACAGATAT CCAAAACAGC 
TCCAAGAGGG AGACTGATGG TTTAAAGCAA GAAATCATGA ATTTAAAGGA AGAACAAAAT 
AAGATGCAAC AGGAAGTTAG TGCCTTATTA CAAGAGAATG AACACCTGAT GGAATTAATG 
AAGACGAAAC ATGAGCATCA ATGTCTAGAA TTGGAACCAA TTCAAGACTC TGAGAAAGAA 
GAGAGGGAGA TAAATACATG TCACCTTCAA CTTCCGATGG ATCTTGATGT TAAAGATACT 
TCTCCAGACA CTTGTAATGC ACAGCTGGTG CAAGTAGAAT CTAAAGTCAG AAACATGGAA 
TTAAAACTTC AGAAGAGTGA GAAGGAAGAG TGTCTACAGT ATGAGTTACA AACTAGAGAA 
TTAGAAACTG AAGATTTGCA ACAAGATCCT CAGTCACAAG ATATTAGTGG CCTTGGAGAC 
TCTGAAATAG ATCCAGAAGA AAAATATATT TCAGTGCTTC AGGAGTTGTC AACAAGTCAG 
CAGGACAATG CCCACCTGCA GTGTTCTCTA CAGACAGCCA TGAACAAGCT GAATGAGTTA 
GAGAAAATGT GTGAAGCCCT GCAGGTTGAA AAGTCTGAAC TCATATCTGA GCTGAATGAC 
TCAAGATCAG AATGTATCAC AGCAACTAGT AAAATGGCAG AAGAGGTAGG GAAACTAGTA 
AATGAAGTTG AAACATTAAA TGACGAAAAT GCACTTCTCC AAGGCGAGTT AGTGGAAGAA 
ACACCAGAAG GTGAATTTGG TGAACAACAA GATGAGCAGA CATCTGTGAA CTTAAATCCT 
TTGGATGACA GTAATTTCTA TGAGCATTTG ACCATGTCAA GCAAAGAGGT TCAAATGCAC 
TTTGCTGAAT TACAGGAGAA ATTTTCATCT TTACAGAGTG AGCACAAAAT TTTACACGAA 
CAGCACTGTC TGATGAGCTC TAAGATGTCA GAGCTGCAGT CCTACGTCGA CACGTTAAAG 
GCTGAAAATT CAGTCTTGTC GATGAGTCTG AGAAGCTCTC AAGGTGACTT GGTAAAGGAG 
GTGCCACCAG GACCTGGGGA GGAGCATTTA CTGTCCTTGT CATTTTCTTG TGTGACTGAC 
AGCCCTGGTA AGGCAAGTTT GGGAGAGTCC TCTTTTTACA AAGATCTTCT AGAGCACACA 
ACAGAAACGT CCCTTTTGAA TAATCTAGAA GGGACTGTTT CGGCAAACCA GTCCAATGTA 
GAGGAAGTCT CTTGTAGTAG TCTGGAGGAG GAGAATCTGA CCGAGAAAGA GATCCCCTCT 
GTCCCACTGA GGAGCGCTCG AGAACTTGAG ACCCTCTGTC AGACGTACCT CGAGTCCCTC 
AAGCAGCTGG AAGAGGAAAT CGAAAGTCAA GGGATTACGA AAAACAAGGA GATCAAAGAG 
CTCAAGGAGC TACTGAGTTC TTCGAGGGAG GAGCTTGACG ACCTCAGGAA GCAGTATTTG 
TCAGAAAATG AACAGTGGCA GCAGAAGCTG ACAGATGTGA CAGCGGAGAT GGAGTCCAAG 
TTGGCGGCTG AAAAGAGACA CGCAGAGCAC CTTACACTTG AGCTTGAGGT GGCGCGGCTT 
CAGCTGCAGG GTCTGGACTT AAGCTCTCGG TCTCTGCTTG GCACCGACAT AGAAGATGCT 
ATTCTGGGTG GAAACGATAG CTGTGACATA AGAGAATCAG AAGAATATAT TTCAGAAACT 
AAAGAGAGGA CCCCAAAGCA TGAGATTCAC CAGATTTGTG AAAAAGATGT TCAGCAGGAC 
CTCAGTCTAG AGATGGAGAA AATAACAAAG ACTGGTGCAA CCAAACTTAC AGAAGAATGG 
TCCAGAGAGC AGTCCTCAGA AAGCAGTCAT GAGACCCCAG TGGAAGATCC AGCCCAGGGC 
TGTTCAGGAT GCATTTCTGA ACTGTCCCTT TCTGGGCCTA ATGCTTCAGT GCCTAGGGAT 
TTTCTGGAGA ATCAGGTAAC CATTCAGAGT CTCGAGCTGA AAGTAAAAGA GACATCAAAT 
GAGAATTTGA GATTACTTCA TGGCATAGAG GAGCGTGACC AAAAAGTTGA AAACTTGCTA 
AATGAAATCA AAGAGTTAGA CTCAAAATTC CATTTACTGG AAGTACAATT AACTACCAAG 
ATTGAAGCAT GTGTGGCATT GGAAAAAATA GTTGAGGAAC TTAAGAAAGA AAAATTAGAT 
TTAAATGAAA AATTGGAATC CTTTTCTTGT CATAACCAGA GAGAAGAAAG TTCAGGAGGT 
CTCACTTCCA ACTTAGAAAT GGTCACAAGC AAATTTCCAC ATGAAGGTAT TGAAGATGAT 
GTAGCCAAGG TGACTGACAA CTGGAGAGAG AAATGTCTCC AGGTGGAAAA TGAGCTACAG 
AGGATTCAAT CTGAGAAAGA TAGCATGGAG CATCATGCCC TCTCTGCTGA AGCCAGCTTA 
GAGGCAGTTC AAACAGAGAA ACTATATTTA GAAAAAGACA ATGAAAATAA ACAAACAGTT 
ATAACCTGTC TTGAGGAAGG ACTCTCAGTG GTCACAAGTG AGAGAGATCA GCTTCGTGGA 
GAGTTAGATA CTTTGTCAAA AGAAAATCAA GAGCTAGATC AGATGTCTGA AAAGATGAAG 
GAGAAAATAC GGGAGCTTGA ATCTCATCAA AGTGAGTGTC TGCATCTCCA AGAGTGGCTT 
CAGAGTTTGG AAAAGGACTC ACAGGCACTG TCTCTGGTAA GAAGTGAGCT GGAAAACCAA 
ATTGAACAAC TGAATAAAGA GAAAGACTCA CTTGTATGGG AATCCGAAAG CCTGCAGACC 
AAACTGAGCG AATCGGAGCA TGAAAAGCTG GCCATCACCA AGGCTTTGGA GGCTGCCCTG 
ATGGAGAAGG GTGAGGTCGC GGTGAGGCTG AGCTCCACGC AAGAGGAAGT GCACCAGTTG 
AGAAAAGGCA TTGAGAAACT CAGGGTCCGC ATTGAGGCTG ATGAAAAGAA GCAGCTCCAT 
GTCTCAGAGA AACTGAAAGA AAGCGAGCGG AGGAATGACT CGCTTCAGGA CAAGGTTGAG 
ACCCTTGAAA GGGAGTTGCA GATGGCAGAA GAAAACCAAG AGCTGGTGAT TCTTGATGCT 
GAGAATTGCA AAGCAGAGGT GGAAACCCTA AAAACACAAA TAGAATTGAT GACTGAACGC 
CTAAAAGATT TAGAATTAGA CCTTGTCACA ATACGGTCTG AAAAAGAAAA TCTTTTGAAA 
CAGCTACAAG AGAAACAAGG TCAGGCGTCT GAATTAGATA CGCTACTCTC TTCATTGAAA 
AACCTATTAG AAGAAAAGGA GCGAGAGAAA ATACAGACGA AAGAAGAAGC TAAAAGTGCA 
GTGGAGATGC TGCAAACACA GTTGAGAGAG CTAACTGAGG AAATAGCAGC CTTATGTGAT 
GACCAGGAGA CATGGAAGGT CGAAGAACAG AGTCTGGACT CTCCTGCCCA GGAGGTACAA 
CAGCTGAGAA ATAACATTGA AAAGCTGAAA GTCCACTTGG ACATTGATAA AAAGAAGCAG 
CTCCAAATCC TAGAAAAACT GAAGGAAAGT GAGCATCAGG CCGATTTCCT TAAGGATACA 
GTTGAGAATC TTGAAAGAGA ACTCAAGCTA TCAGGAGAAA ACCAAGAGCA TGTGACTCTT 
GAGGCTGAGA AGTCCAAAGC AGAAGTAGAG ACCCTGAAAG CAACAGTGGA GAAGATGGAC 
CAAAATCTGA GAGGTTTGCA ATTAGATCTT GTCAATATAA GATCAGAAAA AGAAGATCTG 
ACTAAAGAAT TACAGAAAGA GCAGAGTCGA GTATCTGAAT TAGAAACGTT AAATTCTTCA 
TTTGAAAACC TATTGCGGGA AAAAGAGCAA GAAAAAGTAC AGATGAAGGA AGAATCTAAA 
GCTGCAGTGG AGATGCTGCA AACACAGCTG AAAGAGCTAA GTGAAGAAGT AGCAATCTTG 
TGTGTTGACC AGGAGACCTG GAAGGTTGAA GAACAGAGTC TGGACTCCCC ACTTGAGGAG 
ATACAACAGC TGAGAAATAA CATTGGAAAG CTGAAAGTCT GCCTAGATGC TGATAAAAAG 
CAGCAGCTCC ATATCTTAGA AAAACTGAAG GAAAGTGAGC ATCAGGCCGA TTTCCTTAAG 
GATACAGTTG AGAACCTTGA AAGAGAACTC AAGCTATCAG GAGAAAACCA AGAGCATGTG 
ACTCTTGAGG CTGAGAAGTC CAAAGCAGAA GTAGAGACCC TGAAAGCAAC AGTGGAGGAG 
ATGGACCAAA ATCTGAGAGG TTTGCAATTA GATCTTGTCA ATATAAGATC AGACAAAGAA 
GATCTGACTA AAGAATTACA GAAAGAGCAG AGTCGAGTGT CTGAATTAGA AACGTTAAAT 
TCTTCATTTG AAAACCTATT GCGGGAAAAA GAGCAAGAAA AAGTACAGAT GAAGGAAGAA 
TCTAAAGCTG CAGTGGAGAT GCTGCAAAAA GAATTAGAAG AGCTAAACGA GAAAATGGCA 
GCCTTGGGTA ATGACCAAGA GACCCGGAAG GTCAAAGAAC AGAGCCTGAG TAGTCAAGTA 
GATTCCCTTG AACATGAGAA GGCTCAGTTG CTGCAGGACC TTGATGATGC CAAAAGTAAT 
TACATGATTT TGCAGTCTTC TGTGAATGAC CTCATTCAAG AAGTTGAAGA TGGCAAACAG 
AAACTAGAGA AAAAGGATGA AGAAATCAGT ATACTAAAAA GTCAGACTCG GGACCAAGAG 
CAGCTTGTCT CTAAACTGTC CCAGATGGAA GGAGAACAGC AACTTTGGAA GAAGCAAAAG 
GCAGACCTGG AAAATCTGAT GGTGGAATTG GAGCAGAAGA TTCAGGTGCT GCAATCCAAA 
AACGATGCTT TGCAGGACAC CTTAGAAGCT CTGCAGAATT CTTCCAGGAA TCTGGAGAAA 
GAGCTTGAAT TGACAAAATT GGAAAAAATG TCCTTTGTTG AAAAAGTAAA CACAATGACT 
GTGAAGGAAA CTGAGCTTCA GAAGGAAATA CATGCAGTGA TACAAAAAAC GGTGGCACTG 
AAAGAAGAAT TTAGTGGGGA GAAAAACAGG CTAATGGAAG AATTAAATTT AATGTTGGAA 
GAAGTGAAGA GCAGCAAAGG TCAACTGAAG GAGCTCATGC TAGAAAATAG TGAATTGAAG 
AAGAGTTTGG ATTGTGTACA CAAAGACCGT ATGGAAGAAC AAGGGAAAAT GAGAGGAGAA 
ATAGCTGAAT ATCAGCTACG GCTTCAGGAA GCTGAAAACA AACATCAGGC TTTGCTTCTA 
GATACAAACA AACAGCATGA AATGGAAATC CAGACATACC GAGAGAAACT GACTTCTAAG 
GAGGAATGTC TCAGCTCACA GAAGGTGGAG ATAGACCTCT TAAAGTCCAG TAAAGAAGAA 
CTCAATAATT CTTTGAAAGC GACCACTGAG ATTTTAGAAG AATTGAAGAA AACCAAGATG 
GAAAATCTAA AACATGCAGA TAAATTGAAG AAGGAAAACG ATCGTGCCCA GAGTAAAATA 
AAGTTGTTGG TGAAATCCTG TAAACAGCTG GAAGAGGAAA AGGTGATGTT GCAGAAAGAA 
CTATCTCATC TTGAAGCTGC ACAGGAGAAA CAGAGAGCAG ATACCGTCGT GGATGCTAAT 
GTCGACGAAT TAATAACTGA AATGAAAGAA CTGAAAGAAA CCCTTGAAGA AAAAACCAAG 
GAGGCAGACG AATACCTGGA TAAATACTGT TCCCTGCTCA TAAGCCATGA GAAGTTAGAG 
AAAGCCAAGG AGATGTTAGA AACACAAGTT GCTCGTCTGA GTTCACAACA ATCTAAACTG 
AATCTTCGGA GATCTCCTTT GGTGAATTCG CTTGCTCCAG GACCATCCCC AGTCCCTTCT 
GCCACTGAGA AGAAGTTACC ATCCGGCCAA AATAAATCTT CAGGCAAAAG GCAAAGGTCC 
AGTGGGATTC GGGAGGATGG CGGAGAAACC ACGCCTTCTA CACCAGAGAC ATTTTCTAAA 
AAAAGCAGGA AAGCCATCAG AAGTGGTACT CACCTTGCAG AGGATGCAGA AGATGCTGAA 
TTTGAGCCAG AGGGGCTTCC AGAAGTTGTA AAGAAAGGGT TTGCCGACAT CCCAACCGGA 
AAGACTAGCC CCTACATCCT GCGGAGGACA ACCATGGCAA CCAGGACCAG CCCCCGCCTT 
GCTGCCCAGA AGTTAGCAGC ATCCCCGCCA AGTCTCGACA AAGAAAATCT CACCGAGACC 
TCCAAACCAA CAGCTGGTGG CAGCAGATCA CAAAAGGTCA AGGTTTCTCA ACAGAGCCCA 
GTGGATTCAG GCACTGCCTT CCGAGATCCC ATTGGCAGAT CTCTCTCCAT CAGTAATCTT 
CCCGAGAGAA GTCCAGTGGG CAGCCCCAGG GAGGGCCTGA GAGCCAAGCG AGGCCGCCAC 
GCCCCCAGCC CAGAATCCAG CCTGGAGTCC AAGGGCAGCG AGAACTGTAG GGTCCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Silencing CENPF in bovine preimplantation embryo induces arrest at 8-cell stage.
Reproduction (Cambridge, England)138(5)783-91(2009 Nov)
Toralová T,Susor A,Nemcová L,Kepková K,Kanka J

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Marçais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Unstable microtubule capture at kinetochores depleted of the centromere-associated protein CENP-F.
The EMBO journal24(22)3927-39(2005 Nov)
Bomont P,Maddox P,Shah JV,Desai AB,Cleveland DW

Silencing mitosin induces misaligned chromosomes, premature chromosome decondensation before anaphase onset, and mitotic cell death.
Molecular and cellular biology25(10)4062-74(2005 May)
Yang Z,Guo J,Chen Q,Ding C,Du J,Zhu X

Mitosin/CENP-F as a negative regulator of activating transcription factor-4.
The Journal of biological chemistry280(14)13973-7(2005 Apr)
Zhou X,Wang R,Fan L,Li Y,Ma L,Yang Z,Yu W,Jing N,Zhu X

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CENPF cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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