scn3a cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	scn3a
Entrez Gene ID	100125099
Full Name	sodium channel, voltage gated, type III alpha subunit
Synonyms	na(v)1.3,nav1.3
General protein information	Preferred Names sodium channel, voltage gated, type III alpha subunit Names sodium channel protein type 2 subunit alpha
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031892885.1	XP_031748745.1	sodium channel protein type 2 subunit alpha isoform X4
XM_012971260.3	XP_012826714.1	sodium channel protein type 2 subunit alpha isoform X1
XM_031892883.1	XP_031748743.1	sodium channel protein type 2 subunit alpha isoform X2
XM_012971260.2	XP_012826714.1	sodium channel protein type 2 subunit alpha
XM_031892886.1	XP_031748746.1	sodium channel protein type 2 subunit alpha isoform X5
XM_031892884.1	XP_031748744.1	sodium channel protein type 2 subunit alpha isoform X3

Pathways from BioSystems

Homologies

Homo sapiens (human)	SCN2A	NP_001035233.1
Gallus gallus (chicken)	SCN2A	XP_004942844.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004707	XP_554647.3
Macaca mulatta (Rhesus monkey)	SCN2A	XP_001099916.1
Canis lupus familiaris (dog)	SCN2A	XP_535939.2
Rattus norvegicus (Norway rat)	Scn2a1	NP_036779.1
Drosophila melanogaster (fruit fly)	para	NP_001188624.1
Xenopus tropicalis (tropical clawed frog)	scn3a	XP_002939317.2
Mus musculus (house mouse)	Scn2a1	NP_001092768.1
Bos taurus (cattle)	SCN2A	NP_001137581.1
Pan troglodytes (chimpanzee)	SCN2A	XP_001153227.1
Danio rerio (zebrafish)	scn1lab	NP_001038360.1

Gene Ontology

The following scn3a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the scn3a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa30682	XM_012971260.2 Latest version!	Xenopus tropicalis sodium channel, voltage gated, type III alpha subunit (scn3a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa66279	XM_012971260.3 Latest version!	Xenopus tropicalis sodium channel, voltage gated, type III alpha subunit (scn3a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa66280	XM_031892883.1 Latest version!	Xenopus tropicalis sodium channel, voltage gated, type III alpha subunit (scn3a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa66281	XM_031892884.1 Latest version!	Xenopus tropicalis sodium channel, voltage gated, type III alpha subunit (scn3a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa66282	XM_031892885.1 Latest version!	Xenopus tropicalis sodium channel, voltage gated, type III alpha subunit (scn3a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa66283	XM_031892886.1 Latest version!	Xenopus tropicalis sodium channel, voltage gated, type III alpha subunit (scn3a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

scn3a ( XM_012971260.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826714.1 Xenopus tropicalis sodium channel, voltage gated, type III alpha subunit (scn3a), mRNA.

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CloneID	OXa30682
Clone ID Related Accession (Same CDS sequence)	XM_012971260.2
Accession Version	XM_012971260.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6060bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sodium channel protein type 2 subunit alpha
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012971260.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGC CACTGCTACC CCCACCAGGA CCTGACAGCC TTCGACTCCT CACCAGAGAA 
TCTCTTGCTG TTCTTGAAAA GCGCTTTGCA GAAGAAAAAG CCAGGAAGGC ATCACAGAAG 
GATCGCAGAG ATGATGATGA TGAAAATGGA CCCAAGCCTA ATTCGGACTT GGAAGCTGGC 
AAAAACTTAC CATTTATATA TGGAGACATT CCACAGGGAA TGGTGGCAGT ACCAATGGAA 
GACCTGGACC CATATTACAG CAACCAAAAG ACCTTTATAG TATTGAATAG AGGGAAGGCA 
ATCTTCCGAT TCAGTGCCAC ATCTGCCTTG TACATTTTAA CTCCTTTCAA TCCTCTTAGA 
AGAATATCTA TAAAGATTTT GGTACATTCT TTATTCAGCA TGCTCATTAT GTGCACTATT 
TTGACTAATT GCTTCTTCAT GACCATGAGT AACCCACCAG AATGGACCAA GAATGTTGAA 
TATACCTTCA CTGGAATTTA TACATTTGAA TCACTTGTTA AAATACTTGC CAGAGGTTTT 
TGTTTAGAAA GCTTTACTTT TCTACGGGAT CCATGGAATT GGCTTGATTT CAGCGTCATA 
GTCATGGCAT ATGTGACAGA GTTTGTGGAC CTGGGCAATG TCTCAGCATT GAGAACATTC 
AGAGTTCTCC GAGCATTGAA AACAATATCA GTCATTCCAG GTCTGAAGAC TATCGTGGGC 
GCACTGATTC AGTCGGTGAA GAAGCTTTCC GATGTAATGA TTCTCACAGT GTTTTGCCTC 
AGTGTGTTTG CACTAATAGG GCTACAGCTT TTCATGGGGC ACCTGAGGAA TAAGTGTGTA 
AGGTGGCCAC CTGAGTATGA ACCAAATATC ACTTCCTACT TTAATGAAAC TGCTGGCCTC 
TATATTAATG TAACTCTGAA CCCATTCAAT CGTGAAGAAT ACATCCTAGA AGAAACTAAT 
TTTTATTTTT TGCCTGGAAC GAGAGATGCT CTTCTGTGTG GCAACAGCTC CGATGCAGGG 
CAGTGTCCAA AAGGATATGT ATGTTTGAAG ACAGGAAGGA ACCCAAACTA CGGCTACACA 
AGTTTTGACA CATTTAGCTG GGCTTTTTTA TCACTGTTTC GGCTAATGAC TCAGGATTTC 
TGGGAAAATC TTTATCAACT GACACTACGA GCTGCTGGTA AAACCTACAT GATATTTTTC 
GTTTTGGTGA TTTTCCTGGG TTCTTTCTAT TTGATAAACT TGATCTTGGC TGTGGTGGCC 
ATGGCTTATG AAGAGCAGAA TCAGGCAACC TTAGAAGAAG CGGAGCAGAA AGAAGCAGAA 
TTCCAGCTTA TGATGGAGCA ACTGAAAAAA CATCAGGAAG AAATTCAGGC AGCGGCGCTA 
GCAGCAGAAA CAATGGACGG TTCCAGAGAG TTTAGTGGTG GGACAGGCTA TGGTGGGCTT 
TCAGAAAGCT CTTCTGAGGC ATCAAAGCCA AGTTCCAAAA GTGCTAAGGA GAGAAGAAAT 
AGAAGGAAAA AGAGAAAACA AAAAGAGCAG GGTGAGGATG AGAAGGCAGA TGATGAAAAA 
TTCCACAAGT CGGCTTCAGA AACGAGTATA AAGAGGAAAG GCTTTCGTTT TTCTATAGAA 
GGAAACCGAC TGACCTATGA GAAGAAGTAC TCGTCCCCAC ATCAGTCCCG CCTCAGTTTC 
CGTGGCTCCC TCTTTTTCCC AAGGCGTGAT AGCAGAGCGA GCCTGTGCAG CTTCAGAGGC 
CGTGGGAAGG ATGTAGGATC AGAAAATGAC TTTGCGGATG ATGAACACAG CACATTTGAG 
GACAATGAAA GCAGAAGAGG TTCATTGTTC GTTCCACACA GGCATGGAGA GAGGAGGAAC 
AGCAACATAA GTCAAACCAG CAAGGCATCA AGGACCCTGC CGGTGCTGCC AGTGAATGGG 
AAGATGCACA GCACGGTGGA TTGCAATGGA GTGGTCTCTC TACTTGGTGG CCCATCAGGT 
CCAACATCGC CTGCTGGACT ACTTCTGCCA GAGGTGATAA TAGATAAACC AGCTTCTGAT 
GACAATGGAA CTAATACAGA GAATGAACTG AGGAAAAGAC GGCTCAGTTC GTATCATGTT 
TCTATGGATT TTCTGGAAGA TCCGATTCTC AGACAAAGGG CTCTGAGCAT TGCCAGCAAT 
CTAACAAATA CAATGGAAGA ACTTGAAGAA TCCAGGGAAA AATGTCCACC ATGCTGGAAT 
AAATTTGCCA ATACATATCT AATCTGGGAT TGCAGCGACT CTTGGCTAAA GATAAAGCAT 
GTTGTCAACT TGATTGTCAT GGACCCATTT GTGGATCTTG CAATAACAAT CTGCATTGTT 
TTGAACACGT TATTTATGGC AATGGAGCAT TACCCAATAA CCGATGAATT TGCCGGTGCG 
CTCAATGTGG GAAATTTGGT GTTTACAGGT ATTTTTACTG CTGAGATGGT TTTCAAGCTA 
ATTGCCCTAG ATCCCTATTA CTATTTCCAA GTTGGCTGGA ACATATTCGA TGGGGTTATT 
GTCAGTCTCA GCTTGATGGA ACTTGGTCTT TCGGATGTGG AAGGGTTATC AGTCCTTCGA 
TCTTTCAGAT TGCTTCGTGT ATTTAAATTG GCAAAATCCT GGCCTACACT GAACATGCTG 
ATAAAGATTA TTGGCAATTC TGTAGGAGCT TTGGGAAACC TGACTTTGGT GTTGGCTATC 
ATTGTCTTCA TTTTTGCCGT GGTTGGGATG CAGCTGTTTG GTAAAAGTTA CAAGGAATGT 
GTCTGCAAAA TTGCCCCAGA TGACTGTGAG CTTCCTCGCT GGCATATGAA CGACTTCTTC 
CACTCCTTCC TTATTGTGTT CCGAGTTCTG TGTGGGGAAT GGATTGAAAC CATGTGGGAC 
TGTATGGAGG TTGCAGGACA AGCAATGTGT CTTACTGTAT TTATGATGGT GATGGTTATT 
GGAAACCTAG TGGTGTTAAA TTTGTTTTTA GCTTTGCTGC TCAGCTCATT TAGCTCAGAC 
AATCTAGCTG CTACAGAGGA CGATAATGAA ATGAACAATC TACAAATTGC AGTGGCCAGA 
ATGCACGAAG GAATAGCCTA TGTGAAAAGG AAGATGCGGG AATTCATACA AAACGCCTTT 
GTCAAAAAGC AAAAAGCCTT AGAAGAAACC AAACCCTTAG AAGACATGCA GAACCAAAAG 
GACAGCTGCA TTTCCAATCA CACCACTGTG GAAATAAGCA AAGAGTATGA CTACCTGAAA 
GATGGAAATG TGACAACCGC CACCAGTGGC ATAGGCAGCA GTGTGGAAAA GTACATCATA 
GACGAAAGCG ACTACATGTC TTTCATTCAT AACCCAAGCC TAACTGTCAC TGTACCTATC 
GCTGTGGGGG AGTCCGATTT TGAGAATCTG AACACAGAGG AGTTTAGCAG CGAATCAGAC 
TTGGAAGAAA GCAAGGAGAA GCTCAACTCA TCCAGCTCAT CAGAAGGCAG TACGGTGGAC 
ATCAGGCCTG CTGGGGAGGG AGAGCCTGTT CCACAGGTGG AGGTTGAAGA GTTCCAGGAA 
CCGGATGCTT GTTTCACAGA TGGCTGTATC AGGAGATTTC CATGCTGTGT GGTTGATGTC 
TCACATGGCA AATGGAAGCA GTGGTGGAAC CTCAGAAAAA CATGTTTCTT AATCGTAGAA 
CACAACTGGT TTGAGACCTT CATAATCTTT ATGATTCTTA TGAGTAGTGG TGCCCTGGCA 
TTTGAAGATA TCTACATAGA ACAAAGAAAA ACAATAAAAA TAATTTTGGA ATATGCGGAC 
AAGGTTTTTA CGTACATCTT TATCTTGGAA ATGCTTCTGA AATGGGTGGC ATATGGCTTT 
CAAAAATATT TCACAAATGC CTGGTGCTGG TTGGACTTCT TGATTGTTGA TGTTTCTTTG 
GTTAGCTTAA TAGCTAATGC CTTGGGCTAC TCAGAATTAG GAGCTATTAA GTCCCTTAGG 
ACACTAAGGG CATTGAGACC TCTGAGGGCC CTCTCACGGT TTGAAGGGAT GAGGGTTGTA 
GTGAATGCCC TCGTTGGAGC TATTCCATCC ATTATGAACG TTCTTCTGGT TTGTTTAATA 
TTTTGGCTCA TTTTTAGCAT TATGGGAGTA AACATGTTTT CCGGAAAGTA TGGCTATTGT 
GTGAATTACA CAGATGACGT AGTGTTTGAC TACACAATAG TTAACAATAG GAGCCAATGT 
ATGGACCTAA TTAGTGAAAA TAAAACGGCC AGATGGAAAA ACGTAAAAGT GAATTTTGAT 
AACGTGGGAT TTGGATATCT TGCTTTGCTT CAAGTGGCAA CATTCAAAGG ATGGATGGAA 
ATAATGTATG CAGCAGTTGA CTCAAGAAAG CAAGAGGAAC AGCCAAAATA TGAAGACAAT 
CTGTACATGT ATTTGTACTT CGTTATTTTC ATCATTTTTG GATCGTTTTT CACACTCAAC 
CTTTTCATTG GTGTCATCAT TGATAATTTT AATCAGCAGA AGAAGAAGTT CGGAGGTCAA 
GACATCTTTA TGACAGAAGA ACAAAAAAAA TATTACAATG CAATGAAGAA GCTGGGGTCC 
AAGAAACCAC AAAAGCCTGT TCCAAGACCT GATAATAAAT TTCAAGGTGC CGTCTTTGAT 
TTTGTGTCGA AACAACCGTT TGATATAGCC ATCATGATTC TGATCTGTTT AAATATGGTA 
ACCATGATGA TTGAAACAGA TGATCAGAGC ATCGAAACGG AGAACAACTT GTACTGGATA 
AATGCAGTTT TCATTGTTCT TTTTACTGGG GAGTGTGTGC TCAAGCTGAT ATCCCTACGT 
CATTATTACT TCACAATTGG CTGGAACATA TTTGATTTTG TGGTTGTAAT TCTCTCTATA 
GTAGGTATGT TTCTGGCAGA CCTTATCGAA AAATATTTTG TGTCACCCAC CCTGTTCAGG 
GTCATCCGAT TAGCTAGGAT TGGTCGTATC CTGCGTCTCA TCAAGGGAGC TAAAGGCATT 
CGTACTCTGC TGTTTGCCCT GATGATGTCA CTTCCTGCGT TGTTCAATAT TGGTTTACTG 
CTCTTTTTGG TGATGTTCAT CTACGCCATT TTCGGCATGT CCAACTTTGC CTATGTTAAG 
AAAGAGGCTG GGATTGATGA CATGTTTAAC TTTGAGACCT TTGGTAACAG CATGATCTGT 
TTATTCCAAA TTACAACATC TGCAGGATGG GATGGTCTGT TAGCGCCAAT TCTTAATAGC 
GGGGCACCAG ATTGCGATCC AAAGGCAGAA CATTCAGGAA GCAGTGTAAA AGGAGACTGT 
GGCAATCCCT CTGTTGGCAT CTTCTTTTTT GTTAGTTATA TCATTATATC ATTCTTGGTT 
GTAGTCAACA TGTATATTGC TGTCATCTTG GAAAACTTCA GTGTTGCTAC GGAGGAAAGT 
GCCGAACCTC TAGGAGAAGA TGATTTTGAG ATGTTCTATG AGGTCTGGGA AAAATTTGAT 
TCAAATGCAA CACAGTTTAT ACAATACGTT AAACTGACTG ATTTTGCTGA TGCGCTGGAT 
CCGCCTCTCC GAATGCCGAA ACCAAACAAT GCGCAGTTAA TCGCAATGGA TCTGCCTATG 
GTAAGTGGGG ACAGAATTCA CTGCCTTGAT GTCCTGTTTG CCTTTACAAA GCGTGTGCTA 
GGTGAAGGCG ATGAAATGGA TAACTTAAGA CAGCCAATGG AAGAACGTTT CATGGCATCC 
AATCCTTCAA AGGCCTCCTA CGAACCAATC ACGTCTACTC TACGTAGGAA ACAAGAGGAG 
TTAGCTGCCA TAGTTATCCA GCGCTGCTAC AGATGCTACG CTTTAAGGAA AGGCATAAAA 
CATGCCTCCT TTATGTACAA CCAGGAAAAG GGCAAAGATG GAGGGAGTCT ACTTACTAGG 
AAAGATATGC TTAGCGATCG ACTAAATGGA AACTCTACAA CAGAGAAAGC AGACATTACC 
CCTTCGACAA CGTCTCCTCC GTCCTATGAC AGTGTTACAA AACCAGATAA AGAAAAACAC 
GAAAAAGACA AAATAGAGAA GGAAGACAGG GGCAAAGATG TCAGGGAGCA TAAAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826714.1
CDS	262..6321
Translation	MEQPLLPPPGPDSLRLLTRESLAVLEKRFAEEKARKASQKDRRDDDDENGPKPNSDLEAGKNLPFIYGDIPQGMVAVPMEDLDPYYSNQKTFIVLNRGKAIFRFSATSALYILTPFNPLRRISIKILVHSLFSMLIMCTILTNCFFMTMSNPPEWTKNVEYTFTGIYTFESLVKILARGFCLESFTFLRDPWNWLDFSVIVMAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGHLRNKCVRWPPEYEPNITSYFNETAGLYINVTLNPFNREEYILEETNFYFLPGTRDALLCGNSSDAGQCPKGYVCLKTGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQLMMEQLKKHQEEIQAAALAAETMDGSREFSGGTGYGGLSESSSEASKPSSKSAKERRNRRKKRKQKEQGEDEKADDEKFHKSASETSIKRKGFRFSIEGNRLTYEKKYSSPHQSRLSFRGSLFFPRRDSRASLCSFRGRGKDVGSENDFADDEHSTFEDNESRRGSLFVPHRHGERRNSNISQTSKASRTLPVLPVNGKMHSTVDCNGVVSLLGGPSGPTSPAGLLLPEVIIDKPASDDNGTNTENELRKRRLSSYHVSMDFLEDPILRQRALSIASNLTNTMEELEESREKCPPCWNKFANTYLIWDCSDSWLKIKHVVNLIVMDPFVDLAITICIVLNTLFMAMEHYPITDEFAGALNVGNLVFTGIFTAEMVFKLIALDPYYYFQVGWNIFDGVIVSLSLMELGLSDVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKIAPDDCELPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSSDNLAATEDDNEMNNLQIAVARMHEGIAYVKRKMREFIQNAFVKKQKALEETKPLEDMQNQKDSCISNHTTVEISKEYDYLKDGNVTTATSGIGSSVEKYIIDESDYMSFIHNPSLTVTVPIAVGESDFENLNTEEFSSESDLEESKEKLNSSSSSEGSTVDIRPAGEGEPVPQVEVEEFQEPDACFTDGCIRRFPCCVVDVSHGKWKQWWNLRKTCFLIVEHNWFETFIIFMILMSSGALAFEDIYIEQRKTIKIILEYADKVFTYIFILEMLLKWVAYGFQKYFTNAWCWLDFLIVDVSLVSLIANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNMFSGKYGYCVNYTDDVVFDYTIVNNRSQCMDLISENKTARWKNVKVNFDNVGFGYLALLQVATFKGWMEIMYAAVDSRKQEEQPKYEDNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPVPRPDNKFQGAVFDFVSKQPFDIAIMILICLNMVTMMIETDDQSIETENNLYWINAVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLADLIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKKEAGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSGAPDCDPKAEHSGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLGEDDFEMFYEVWEKFDSNATQFIQYVKLTDFADALDPPLRMPKPNNAQLIAMDLPMVSGDRIHCLDVLFAFTKRVLGEGDEMDNLRQPMEERFMASNPSKASYEPITSTLRRKQEELAAIVIQRCYRCYALRKGIKHASFMYNQEKGKDGGSLLTRKDMLSDRLNGNSTTEKADITPSTTSPPSYDSVTKPDKEKHEKDKIEKEDRGKDVREHKK

Target ORF information:

RefSeq Version	XM_012971260.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sodium channel, voltage gated, type III alpha subunit (scn3a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971260.2

ORF Insert Sequence:

ATGGAGCAGC CACTGCTACC CCCACCAGGA CCTGACAGCC TTCGACTCCT CACCAGAGAA 
TCTCTTGCTG TTCTTGAAAA GCGCTTTGCA GAAGAAAAAG CCAGGAAGGC ATCACAGAAG 
GATCGCAGAG ATGATGATGA TGAAAATGGA CCCAAGCCTA ATTCGGACTT GGAAGCTGGC 
AAAAACTTAC CATTTATATA TGGAGACATT CCACAGGGAA TGGTGGCAGT ACCAATGGAA 
GACCTGGACC CATATTACAG CAACCAAAAG ACCTTTATAG TATTGAATAG AGGGAAGGCA 
ATCTTCCGAT TCAGTGCCAC ATCTGCCTTG TACATTTTAA CTCCTTTCAA TCCTCTTAGA 
AGAATATCTA TAAAGATTTT GGTACATTCT TTATTCAGCA TGCTCATTAT GTGCACTATT 
TTGACTAATT GCTTCTTCAT GACCATGAGT AACCCACCAG AATGGACCAA GAATGTTGAA 
TATACCTTCA CTGGAATTTA TACATTTGAA TCACTTGTTA AAATACTTGC CAGAGGTTTT 
TGTTTAGAAA GCTTTACTTT TCTACGGGAT CCATGGAATT GGCTTGATTT CAGCGTCATA 
GTCATGGCAT ATGTGACAGA GTTTGTGGAC CTGGGCAATG TCTCAGCATT GAGAACATTC 
AGAGTTCTCC GAGCATTGAA AACAATATCA GTCATTCCAG GTCTGAAGAC TATCGTGGGC 
GCACTGATTC AGTCGGTGAA GAAGCTTTCC GATGTAATGA TTCTCACAGT GTTTTGCCTC 
AGTGTGTTTG CACTAATAGG GCTACAGCTT TTCATGGGGC ACCTGAGGAA TAAGTGTGTA 
AGGTGGCCAC CTGAGTATGA ACCAAATATC ACTTCCTACT TTAATGAAAC TGCTGGCCTC 
TATATTAATG TAACTCTGAA CCCATTCAAT CGTGAAGAAT ACATCCTAGA AGAAACTAAT 
TTTTATTTTT TGCCTGGAAC GAGAGATGCT CTTCTGTGTG GCAACAGCTC CGATGCAGGG 
CAGTGTCCAA AAGGATATGT ATGTTTGAAG ACAGGAAGGA ACCCAAACTA CGGCTACACA 
AGTTTTGACA CATTTAGCTG GGCTTTTTTA TCACTGTTTC GGCTAATGAC TCAGGATTTC 
TGGGAAAATC TTTATCAACT GACACTACGA GCTGCTGGTA AAACCTACAT GATATTTTTC 
GTTTTGGTGA TTTTCCTGGG TTCTTTCTAT TTGATAAACT TGATCTTGGC TGTGGTGGCC 
ATGGCTTATG AAGAGCAGAA TCAGGCAACC TTAGAAGAAG CGGAGCAGAA AGAAGCAGAA 
TTCCAGCTTA TGATGGAGCA ACTGAAAAAA CATCAGGAAG AAATTCAGGC AGCGGCGCTA 
GCAGCAGAAA CAATGGACGG TTCCAGAGAG TTTAGTGGTG GGACAGGCTA TGGTGGGCTT 
TCAGAAAGCT CTTCTGAGGC ATCAAAGCCA AGTTCCAAAA GTGCTAAGGA GAGAAGAAAT 
AGAAGGAAAA AGAGAAAACA AAAAGAGCAG GGTGAGGATG AGAAGGCAGA TGATGAAAAA 
TTCCACAAGT CGGCTTCAGA AACGAGTATA AAGAGGAAAG GCTTTCGTTT TTCTATAGAA 
GGAAACCGAC TGACCTATGA GAAGAAGTAC TCGTCCCCAC ATCAGTCCCG CCTCAGTTTC 
CGTGGCTCCC TCTTTTTCCC AAGGCGTGAT AGCAGAGCGA GCCTGTGCAG CTTCAGAGGC 
CGTGGGAAGG ATGTAGGATC AGAAAATGAC TTTGCGGATG ATGAACACAG CACATTTGAG 
GACAATGAAA GCAGAAGAGG TTCATTGTTC GTTCCACACA GGCATGGAGA GAGGAGGAAC 
AGCAACATAA GTCAAACCAG CAAGGCATCA AGGACCCTGC CGGTGCTGCC AGTGAATGGG 
AAGATGCACA GCACGGTGGA TTGCAATGGA GTGGTCTCTC TACTTGGTGG CCCATCAGGT 
CCAACATCGC CTGCTGGACT ACTTCTGCCA GAGGTGATAA TAGATAAACC AGCTTCTGAT 
GACAATGGAA CTAATACAGA GAATGAACTG AGGAAAAGAC GGCTCAGTTC GTATCATGTT 
TCTATGGATT TTCTGGAAGA TCCGATTCTC AGACAAAGGG CTCTGAGCAT TGCCAGCAAT 
CTAACAAATA CAATGGAAGA ACTTGAAGAA TCCAGGGAAA AATGTCCACC ATGCTGGAAT 
AAATTTGCCA ATACATATCT AATCTGGGAT TGCAGCGACT CTTGGCTAAA GATAAAGCAT 
GTTGTCAACT TGATTGTCAT GGACCCATTT GTGGATCTTG CAATAACAAT CTGCATTGTT 
TTGAACACGT TATTTATGGC AATGGAGCAT TACCCAATAA CCGATGAATT TGCCGGTGCG 
CTCAATGTGG GAAATTTGGT GTTTACAGGT ATTTTTACTG CTGAGATGGT TTTCAAGCTA 
ATTGCCCTAG ATCCCTATTA CTATTTCCAA GTTGGCTGGA ACATATTCGA TGGGGTTATT 
GTCAGTCTCA GCTTGATGGA ACTTGGTCTT TCGGATGTGG AAGGGTTATC AGTCCTTCGA 
TCTTTCAGAT TGCTTCGTGT ATTTAAATTG GCAAAATCCT GGCCTACACT GAACATGCTG 
ATAAAGATTA TTGGCAATTC TGTAGGAGCT TTGGGAAACC TGACTTTGGT GTTGGCTATC 
ATTGTCTTCA TTTTTGCCGT GGTTGGGATG CAGCTGTTTG GTAAAAGTTA CAAGGAATGT 
GTCTGCAAAA TTGCCCCAGA TGACTGTGAG CTTCCTCGCT GGCATATGAA CGACTTCTTC 
CACTCCTTCC TTATTGTGTT CCGAGTTCTG TGTGGGGAAT GGATTGAAAC CATGTGGGAC 
TGTATGGAGG TTGCAGGACA AGCAATGTGT CTTACTGTAT TTATGATGGT GATGGTTATT 
GGAAACCTAG TGGTGTTAAA TTTGTTTTTA GCTTTGCTGC TCAGCTCATT TAGCTCAGAC 
AATCTAGCTG CTACAGAGGA CGATAATGAA ATGAACAATC TACAAATTGC AGTGGCCAGA 
ATGCACGAAG GAATAGCCTA TGTGAAAAGG AAGATGCGGG AATTCATACA AAACGCCTTT 
GTCAAAAAGC AAAAAGCCTT AGAAGAAACC AAACCCTTAG AAGACATGCA GAACCAAAAG 
GACAGCTGCA TTTCCAATCA CACCACTGTG GAAATAAGCA AAGAGTATGA CTACCTGAAA 
GATGGAAATG TGACAACCGC CACCAGTGGC ATAGGCAGCA GTGTGGAAAA GTACATCATA 
GACGAAAGCG ACTACATGTC TTTCATTCAT AACCCAAGCC TAACTGTCAC TGTACCTATC 
GCTGTGGGGG AGTCCGATTT TGAGAATCTG AACACAGAGG AGTTTAGCAG CGAATCAGAC 
TTGGAAGAAA GCAAGGAGAA GCTCAACTCA TCCAGCTCAT CAGAAGGCAG TACGGTGGAC 
ATCAGGCCTG CTGGGGAGGG AGAGCCTGTT CCACAGGTGG AGGTTGAAGA GTTCCAGGAA 
CCGGATGCTT GTTTCACAGA TGGCTGTATC AGGAGATTTC CATGCTGTGT GGTTGATGTC 
TCACATGGCA AATGGAAGCA GTGGTGGAAC CTCAGAAAAA CATGTTTCTT AATCGTAGAA 
CACAACTGGT TTGAGACCTT CATAATCTTT ATGATTCTTA TGAGTAGTGG TGCCCTGGCA 
TTTGAAGATA TCTACATAGA ACAAAGAAAA ACAATAAAAA TAATTTTGGA ATATGCGGAC 
AAGGTTTTTA CGTACATCTT TATCTTGGAA ATGCTTCTGA AATGGGTGGC ATATGGCTTT 
CAAAAATATT TCACAAATGC CTGGTGCTGG TTGGACTTCT TGATTGTTGA TGTTTCTTTG 
GTTAGCTTAA TAGCTAATGC CTTGGGCTAC TCAGAATTAG GAGCTATTAA GTCCCTTAGG 
ACACTAAGGG CATTGAGACC TCTGAGGGCC CTCTCACGGT TTGAAGGGAT GAGGGTTGTA 
GTGAATGCCC TCGTTGGAGC TATTCCATCC ATTATGAACG TTCTTCTGGT TTGTTTAATA 
TTTTGGCTCA TTTTTAGCAT TATGGGAGTA AACATGTTTT CCGGAAAGTA TGGCTATTGT 
GTGAATTACA CAGATGACGT AGTGTTTGAC TACACAATAG TTAACAATAG GAGCCAATGT 
ATGGACCTAA TTAGTGAAAA TAAAACGGCC AGATGGAAAA ACGTAAAAGT GAATTTTGAT 
AACGTGGGAT TTGGATATCT TGCTTTGCTT CAAGTGGCAA CATTCAAAGG ATGGATGGAA 
ATAATGTATG CAGCAGTTGA CTCAAGAAAG CAAGAGGAAC AGCCAAAATA TGAAGACAAT 
CTGTACATGT ATTTGTACTT CGTTATTTTC ATCATTTTTG GATCGTTTTT CACACTCAAC 
CTTTTCATTG GTGTCATCAT TGATAATTTT AATCAGCAGA AGAAGAAGTT CGGAGGTCAA 
GACATCTTTA TGACAGAAGA ACAAAAAAAA TATTACAATG CAATGAAGAA GCTGGGGTCC 
AAGAAACCAC AAAAGCCTGT TCCAAGACCT GATAATAAAT TTCAAGGTGC CGTCTTTGAT 
TTTGTGTCGA AACAACCGTT TGATATAGCC ATCATGATTC TGATCTGTTT AAATATGGTA 
ACCATGATGA TTGAAACAGA TGATCAGAGC ATCGAAACGG AGAACAACTT GTACTGGATA 
AATGCAGTTT TCATTGTTCT TTTTACTGGG GAGTGTGTGC TCAAGCTGAT ATCCCTACGT 
CATTATTACT TCACAATTGG CTGGAACATA TTTGATTTTG TGGTTGTAAT TCTCTCTATA 
GTAGGTATGT TTCTGGCAGA CCTTATCGAA AAATATTTTG TGTCACCCAC CCTGTTCAGG 
GTCATCCGAT TAGCTAGGAT TGGTCGTATC CTGCGTCTCA TCAAGGGAGC TAAAGGCATT 
CGTACTCTGC TGTTTGCCCT GATGATGTCA CTTCCTGCGT TGTTCAATAT TGGTTTACTG 
CTCTTTTTGG TGATGTTCAT CTACGCCATT TTCGGCATGT CCAACTTTGC CTATGTTAAG 
AAAGAGGCTG GGATTGATGA CATGTTTAAC TTTGAGACCT TTGGTAACAG CATGATCTGT 
TTATTCCAAA TTACAACATC TGCAGGATGG GATGGTCTGT TAGCGCCAAT TCTTAATAGC 
GGGGCACCAG ATTGCGATCC AAAGGCAGAA CATTCAGGAA GCAGTGTAAA AGGAGACTGT 
GGCAATCCCT CTGTTGGCAT CTTCTTTTTT GTTAGTTATA TCATTATATC ATTCTTGGTT 
GTAGTCAACA TGTATATTGC TGTCATCTTG GAAAACTTCA GTGTTGCTAC GGAGGAAAGT 
GCCGAACCTC TAGGAGAAGA TGATTTTGAG ATGTTCTATG AGGTCTGGGA AAAATTTGAT 
TCAAATGCAA CACAGTTTAT ACAATACGTT AAACTGACTG ATTTTGCTGA TGCGCTGGAT 
CCGCCTCTCC GAATGCCGAA ACCAAACAAT GCGCAGTTAA TCGCAATGGA TCTGCCTATG 
GTAAGTGGGG ACAGAATTCA CTGCCTTGAT GTCCTGTTTG CCTTTACAAA GCGTGTGCTA 
GGTGAAGGCG ATGAAATGGA TAACTTAAGA CAGCCAATGG AAGAACGTTT CATGGCATCC 
AATCCTTCAA AGGCCTCCTA CGAACCAATC ACGTCTACTC TACGTAGGAA ACAAGAGGAG 
TTAGCTGCCA TAGTTATCCA GCGCTGCTAC AGATGCTACG CTTTAAGGAA AGGCATAAAA 
CATGCCTCCT TTATGTACAA CCAGGAAAAG GGCAAAGATG GAGGGAGTCT ACTTACTAGG 
AAAGATATGC TTAGCGATCG ACTAAATGGA AACTCTACAA CAGAGAAAGC AGACATTACC 
CCTTCGACAA CGTCTCCTCC GTCCTATGAC AGTGTTACAA AACCAGATAA AGAAAAACAC 
GAAAAAGACA AAATAGAGAA GGAAGACAGG GGCAAAGATG TCAGGGAGCA TAAAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

scn3a ( XM_012971260.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826714.1 Xenopus tropicalis sodium channel, voltage gated, type III alpha subunit (scn3a), transcript variant X1, mRNA.

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CloneID	OXa66279
Clone ID Related Accession (Same CDS sequence)	XM_012971260.3
Accession Version	XM_012971260.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6060bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sodium channel protein type 2 subunit alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012971260.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGC CACTGCTACC CCCACCAGGA CCTGACAGCC TTCGACTCCT CACCAGAGAA 
TCTCTTGCTG TTCTTGAAAA GCGCTTTGCA GAAGAAAAAG CCAGGAAGGC ATCACAGAAG 
GATCGCAGAG ATGATGATGA TGAAAATGGA CCCAAGCCTA ATTCGGACTT GGAAGCTGGC 
AAAAACTTAC CATTTATATA TGGAGACATT CCACAGGGAA TGGTGGCAGT ACCAATGGAA 
GACCTGGACC CATATTACAG CAACCAAAAG ACCTTTATAG TATTGAATAG AGGGAAGGCA 
ATCTTCCGAT TCAGTGCCAC ATCTGCCTTG TACATTTTAA CTCCTTTCAA TCCTCTTAGA 
AGAATATCTA TAAAGATTTT GGTACATTCT TTATTCAGCA TGCTCATTAT GTGCACTATT 
TTGACTAATT GCTTCTTCAT GACCATGAGT AACCCACCAG AATGGACCAA GAATGTTGAA 
TATACCTTCA CTGGAATTTA TACATTTGAA TCACTTGTTA AAATACTTGC CAGAGGTTTT 
TGTTTAGAAA GCTTTACTTT TCTACGGGAT CCATGGAATT GGCTTGATTT CAGCGTCATA 
GTCATGGCAT ATGTGACAGA GTTTGTGGAC CTGGGCAATG TCTCAGCATT GAGAACATTC 
AGAGTTCTCC GAGCATTGAA AACAATATCA GTCATTCCAG GTCTGAAGAC TATCGTGGGC 
GCACTGATTC AGTCGGTGAA GAAGCTTTCC GATGTAATGA TTCTCACAGT GTTTTGCCTC 
AGTGTGTTTG CACTAATAGG GCTACAGCTT TTCATGGGGC ACCTGAGGAA TAAGTGTGTA 
AGGTGGCCAC CTGAGTATGA ACCAAATATC ACTTCCTACT TTAATGAAAC TGCTGGCCTC 
TATATTAATG TAACTCTGAA CCCATTCAAT CGTGAAGAAT ACATCCTAGA AGAAACTAAT 
TTTTATTTTT TGCCTGGAAC GAGAGATGCT CTTCTGTGTG GCAACAGCTC CGATGCAGGG 
CAGTGTCCAA AAGGATATGT ATGTTTGAAG ACAGGAAGGA ACCCAAACTA CGGCTACACA 
AGTTTTGACA CATTTAGCTG GGCTTTTTTA TCACTGTTTC GGCTAATGAC TCAGGATTTC 
TGGGAAAATC TTTATCAACT GACACTACGA GCTGCTGGTA AAACCTACAT GATATTTTTC 
GTTTTGGTGA TTTTCCTGGG TTCTTTCTAT TTGATAAACT TGATCTTGGC TGTGGTGGCC 
ATGGCTTATG AAGAGCAGAA TCAGGCAACC TTAGAAGAAG CGGAGCAGAA AGAAGCAGAA 
TTCCAGCTTA TGATGGAGCA ACTGAAAAAA CATCAGGAAG AAATTCAGGC AGCGGCGCTA 
GCAGCAGAAA CAATGGACGG TTCCAGAGAG TTTAGTGGTG GGACAGGCTA TGGTGGGCTT 
TCAGAAAGCT CTTCTGAGGC ATCAAAGCCA AGTTCCAAAA GTGCTAAGGA GAGAAGAAAT 
AGAAGGAAAA AGAGAAAACA AAAAGAGCAG GGTGAGGATG AGAAGGCAGA TGATGAAAAA 
TTCCACAAGT CGGCTTCAGA AACGAGTATA AAGAGGAAAG GCTTTCGTTT TTCTATAGAA 
GGAAACCGAC TGACCTATGA GAAGAAGTAC TCGTCCCCAC ATCAGTCCCG CCTCAGTTTC 
CGTGGCTCCC TCTTTTTCCC AAGGCGTGAT AGCAGAGCGA GCCTGTGCAG CTTCAGAGGC 
CGTGGGAAGG ATGTAGGATC AGAAAATGAC TTTGCGGATG ATGAACACAG CACATTTGAG 
GACAATGAAA GCAGAAGAGG TTCATTGTTC GTTCCACACA GGCATGGAGA GAGGAGGAAC 
AGCAACATAA GTCAAACCAG CAAGGCATCA AGGACCCTGC CGGTGCTGCC AGTGAATGGG 
AAGATGCACA GCACGGTGGA TTGCAATGGA GTGGTCTCTC TACTTGGTGG CCCATCAGGT 
CCAACATCGC CTGCTGGACT ACTTCTGCCA GAGGTGATAA TAGATAAACC AGCTTCTGAT 
GACAATGGAA CTAATACAGA GAATGAACTG AGGAAAAGAC GGCTCAGTTC GTATCATGTT 
TCTATGGATT TTCTGGAAGA TCCGATTCTC AGACAAAGGG CTCTGAGCAT TGCCAGCAAT 
CTAACAAATA CAATGGAAGA ACTTGAAGAA TCCAGGGAAA AATGTCCACC ATGCTGGAAT 
AAATTTGCCA ATACATATCT AATCTGGGAT TGCAGCGACT CTTGGCTAAA GATAAAGCAT 
GTTGTCAACT TGATTGTCAT GGACCCATTT GTGGATCTTG CAATAACAAT CTGCATTGTT 
TTGAACACGT TATTTATGGC TATGGAGCAT TACCCAATAA CCGATGAATT TGCCGGTGCG 
CTCAATGTGG GAAATTTGGT GTTTACAGGT ATTTTTACTG CTGAGATGGT TTTCAAGCTA 
ATTGCCCTAG ATCCCTATTA CTATTTCCAA GTTGGCTGGA ACATATTCGA TGGGGTTATT 
GTCAGTCTCA GCTTGATGGA ACTTGGTCTT TCGGATGTGG AAGGGTTATC AGTCCTTCGA 
TCTTTCAGAT TGCTTCGTGT ATTCAAATTG GCGAAATCCT GGCCTACACT GAACATGCTG 
ATAAAAATTA TTGGCAATTC TGTAGGAGCT TTGGGGAACC TGACTTTGGT GTTGGCCATC 
ATTGTCTTCA TTTTTGCTGT GGTTGGGATG CAGCTGTTTG GTAAAAGTTA CAAGGAATGT 
GTCTGCAAAA TTGCCCCAGA TGACTGTGAG CTTCCTCGCT GGCATATGAA CGACTTCTTC 
CACTCCTTCC TTATTGTGTT CCGAGTTCTG TGTGGGGAAT GGATTGAAAC CATGTGGGAC 
TGTATGGAGG TTGCAGGACA AGCTATGTGT CTTACTGTAT TTATGATGGT GATGGTTATT 
GGAAACCTAG TGGTGTTAAA TTTGTTTTTA GCTTTGCTGC TCAGCTCATT TAGCTCAGAC 
AATCTAGCTG CTACAGAGGA CGATAATGAA ATGAACAATC TACAAATTGC AGTGGCCAGA 
ATGCACGAAG GAATAGCCTA TGTGAAAAGG AAGATGCGGG AATTCATACA AAACGCCTTT 
GTCAAAAAGC AAAAAGCCTT AGAAGAAACC AAACCCTTAG AAGACATGCA GAACCAAAAG 
GACAGCTGCA TTTCCAATCA CACCACTGTG GAAATAAGCA AAGAGTATGA CTACCTGAAA 
GATGGAAATG TGACAACGGC CACCAGTGGC ATAGGCAGCA GTGTGGAAAA GTACATCATA 
GACGAAAGCG ACTACATGTC TTTCATTCAT AACCCAAGCC TAACTGTCAC TGTACCTATT 
GCTGTGGGGG AGTCCGATTT TGAGAATCTG AACACAGAGG AGTTTAGCAG CGAATCAGAC 
TTGGAAGAAA GCAAGGAGAA GCTCAACTCA TCCAGCTCAT CAGAAGGCAG TACGGTGGAC 
ATCAGGCCTG CTGGGGAGGG AGAGCCTGTT CCACAGGTGG AGGTTGAAGA GTTCCAGGAA 
CCGGATGCTT GTTTCACAGA TGGCTGTATC AGGAGATTTC CATGCTGTGT GGTTGATGTC 
TCACATGGCA AATGGAAGCA GTGGTGGAAC CTCAGAAAAA CATGTTTCTT AATCGTAGAA 
CACAACTGGT TTGAGACCTT CATAATCTTT ATGATTCTTA TGAGTAGTGG TGCCCTGGCA 
TTTGAAGATA TCTACATAGA ACAAAGAAAA ACAATAAAAA TAATTTTGGA ATATGCGGAC 
AAGGTTTTTA CGTACATCTT TATCTTGGAA ATGCTTCTGA AATGGGTGGC ATATGGCTTT 
CAAAAATATT TCACAAATGC CTGGTGCTGG TTGGACTTCT TGATTGTTGA TGTTTCTTTG 
GTTAGCTTAA TAGCTAATGC CTTGGGCTAC TCAGAATTAG GAGCTATTAA GTCCCTTAGG 
ACACTAAGGG CATTGAGACC TCTGAGGGCC CTCTCACGGT TTGAAGGGAT GAGGGTTGTA 
GTGAATGCCC TCGTTGGAGC TATTCCATCC ATTATGAACG TTCTTCTGGT TTGTTTAATA 
TTTTGGCTCA TTTTTAGCAT TATGGGAGTA AACATGTTTT CCGGAAAGTA TGGCTATTGT 
GTGAATTACA CAGATGACGT AGTGTTTGAC TACACAATAG TTAACAATAG GAGCCAATGT 
ATGGACCTAA TTAGTGAAAA TAAAACGGCC AGATGGAAAA ACGTAAAAGT GAATTTTGAT 
AACGTGGGAT TTGGATATCT TGCTTTGCTT CAAGTGGCAA CATTCAAAGG ATGGATGGAA 
ATAATGTATG CAGCAGTTGA CTCAAGAAAG CAAGAGGAAC AGCCAAAATA TGAAGACAAT 
CTGTACATGT ATTTGTACTT CGTTATTTTC ATCATTTTTG GATCGTTTTT CACACTCAAC 
CTTTTCATTG GTGTCATCAT TGATAATTTT AATCAGCAGA AGAAGAAGTT CGGAGGTCAA 
GACATCTTTA TGACGGAAGA ACAAAAAAAA TATTACAATG CAATGAAGAA GCTGGGGTCC 
AAGAAACCAC AAAAGCCTGT TCCAAGACCT GATAATAAAT TTCAAGGTGC CGTCTTTGAT 
TTTGTGTCGA AACAACCGTT TGATATAGCC ATCATGATTC TGATCTGTTT AAATATGGTA 
ACCATGATGA TTGAAACAGA TGATCAGAGC ATCGAAACGG AGAACAACTT GTACTGGATA 
AATGCAGTTT TCATTGTTCT TTTTACTGGG GAGTGTGTGC TCAAGCTGAT ATCCCTACGT 
CATTATTACT TCACAATTGG CTGGAACATA TTTGATTTTG TGGTTGTAAT TCTCTCTATA 
GTAGGTATGT TTCTGGCAGA CCTTATCGAA AAATATTTTG TGTCACCCAC CCTGTTCAGG 
GTCATCCGAT TAGCTAGGAT TGGTCGTATC CTGCGTCTCA TCAAGGGAGC TAAAGGCATT 
CGTACTCTGC TGTTTGCCCT GATGATGTCA CTTCCTGCGT TGTTCAATAT TGGTTTACTG 
CTCTTTTTGG TGATGTTCAT CTACGCCATT TTCGGCATGT CCAACTTTGC CTATGTTAAG 
AAAGAGGCTG GGATTGATGA CATGTTTAAC TTTGAGACCT TTGGTAACAG CATGATCTGT 
TTATTCCAAA TTACAACATC TGCAGGATGG GATGGTCTGT TAGCGCCAAT TCTTAATAGC 
GGGGCACCAG ATTGCGATCC AAAGGCAGAA CATTCAGGAA GCAGTGTAAA AGGAGACTGT 
GGCAATCCCT CTGTTGGCAT CTTCTTTTTT GTTAGTTATA TCATTATATC ATTCTTGGTT 
GTAGTCAACA TGTATATTGC TGTCATCTTG GAAAACTTCA GTGTTGCTAC GGAGGAAAGT 
GCCGAACCTC TAGGAGAAGA TGATTTTGAG ATGTTCTATG AGGTCTGGGA AAAATTTGAT 
TCAAATGCAA CACAGTTTAT ACAATACGTT AAACTGACTG ATTTTGCTGA TGCGCTGGAT 
CCGCCTCTCC GAATGCCGAA ACCAAACAAT GCGCAGTTAA TCGCAATGGA TCTGCCTATG 
GTAAGTGGGG ACAGAATTCA CTGCCTTGAT GTCCTGTTTG CCTTTACAAA GCGTGTGCTA 
GGTGAAGGCG ATGAAATGGA TAACTTAAGA CAGCCAATGG AAGAACGTTT CATGGCATCC 
AATCCTTCAA AGGCCTCCTA CGAACCAATC ACGTCTACTC TACGTAGGAA ACAAGAGGAG 
TTAGCTGCCA TAGTTATCCA GCGCTGCTAC AGATGCTACG CTTTAAGGAA AGGCATAAAA 
CATGCCTCCT TTATGTACAA CCAGGAAAAG GGCAAAGATG GAGGGAGTCT ACTTACTAGG 
AAAGATATGC TTAGCGATCG ACTAAATGGA AACTCTACAA CAGAGAAAGC AGACATTACC 
CCTTCGACAA CGTCTCCTCC GTCCTATGAC AGTGTTACAA AACCAGATAA AGAAAAACAC 
GAAAAAGACA AAATAGAGAA GGAAGACAGG GGCAAAGATG TCAGGGAGCA TAAAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826714.1
CDS	285..6344
Translation	MEQPLLPPPGPDSLRLLTRESLAVLEKRFAEEKARKASQKDRRDDDDENGPKPNSDLEAGKNLPFIYGDIPQGMVAVPMEDLDPYYSNQKTFIVLNRGKAIFRFSATSALYILTPFNPLRRISIKILVHSLFSMLIMCTILTNCFFMTMSNPPEWTKNVEYTFTGIYTFESLVKILARGFCLESFTFLRDPWNWLDFSVIVMAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGHLRNKCVRWPPEYEPNITSYFNETAGLYINVTLNPFNREEYILEETNFYFLPGTRDALLCGNSSDAGQCPKGYVCLKTGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQLMMEQLKKHQEEIQAAALAAETMDGSREFSGGTGYGGLSESSSEASKPSSKSAKERRNRRKKRKQKEQGEDEKADDEKFHKSASETSIKRKGFRFSIEGNRLTYEKKYSSPHQSRLSFRGSLFFPRRDSRASLCSFRGRGKDVGSENDFADDEHSTFEDNESRRGSLFVPHRHGERRNSNISQTSKASRTLPVLPVNGKMHSTVDCNGVVSLLGGPSGPTSPAGLLLPEVIIDKPASDDNGTNTENELRKRRLSSYHVSMDFLEDPILRQRALSIASNLTNTMEELEESREKCPPCWNKFANTYLIWDCSDSWLKIKHVVNLIVMDPFVDLAITICIVLNTLFMAMEHYPITDEFAGALNVGNLVFTGIFTAEMVFKLIALDPYYYFQVGWNIFDGVIVSLSLMELGLSDVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKIAPDDCELPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSSDNLAATEDDNEMNNLQIAVARMHEGIAYVKRKMREFIQNAFVKKQKALEETKPLEDMQNQKDSCISNHTTVEISKEYDYLKDGNVTTATSGIGSSVEKYIIDESDYMSFIHNPSLTVTVPIAVGESDFENLNTEEFSSESDLEESKEKLNSSSSSEGSTVDIRPAGEGEPVPQVEVEEFQEPDACFTDGCIRRFPCCVVDVSHGKWKQWWNLRKTCFLIVEHNWFETFIIFMILMSSGALAFEDIYIEQRKTIKIILEYADKVFTYIFILEMLLKWVAYGFQKYFTNAWCWLDFLIVDVSLVSLIANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNMFSGKYGYCVNYTDDVVFDYTIVNNRSQCMDLISENKTARWKNVKVNFDNVGFGYLALLQVATFKGWMEIMYAAVDSRKQEEQPKYEDNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPVPRPDNKFQGAVFDFVSKQPFDIAIMILICLNMVTMMIETDDQSIETENNLYWINAVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLADLIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKKEAGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSGAPDCDPKAEHSGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLGEDDFEMFYEVWEKFDSNATQFIQYVKLTDFADALDPPLRMPKPNNAQLIAMDLPMVSGDRIHCLDVLFAFTKRVLGEGDEMDNLRQPMEERFMASNPSKASYEPITSTLRRKQEELAAIVIQRCYRCYALRKGIKHASFMYNQEKGKDGGSLLTRKDMLSDRLNGNSTTEKADITPSTTSPPSYDSVTKPDKEKHEKDKIEKEDRGKDVREHKK

Target ORF information:

RefSeq Version	XM_012971260.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sodium channel, voltage gated, type III alpha subunit (scn3a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971260.3

ORF Insert Sequence:

ATGGAGCAGC CACTGCTACC CCCACCAGGA CCTGACAGCC TTCGACTCCT CACCAGAGAA 
TCTCTTGCTG TTCTTGAAAA GCGCTTTGCA GAAGAAAAAG CCAGGAAGGC ATCACAGAAG 
GATCGCAGAG ATGATGATGA TGAAAATGGA CCCAAGCCTA ATTCGGACTT GGAAGCTGGC 
AAAAACTTAC CATTTATATA TGGAGACATT CCACAGGGAA TGGTGGCAGT ACCAATGGAA 
GACCTGGACC CATATTACAG CAACCAAAAG ACCTTTATAG TATTGAATAG AGGGAAGGCA 
ATCTTCCGAT TCAGTGCCAC ATCTGCCTTG TACATTTTAA CTCCTTTCAA TCCTCTTAGA 
AGAATATCTA TAAAGATTTT GGTACATTCT TTATTCAGCA TGCTCATTAT GTGCACTATT 
TTGACTAATT GCTTCTTCAT GACCATGAGT AACCCACCAG AATGGACCAA GAATGTTGAA 
TATACCTTCA CTGGAATTTA TACATTTGAA TCACTTGTTA AAATACTTGC CAGAGGTTTT 
TGTTTAGAAA GCTTTACTTT TCTACGGGAT CCATGGAATT GGCTTGATTT CAGCGTCATA 
GTCATGGCAT ATGTGACAGA GTTTGTGGAC CTGGGCAATG TCTCAGCATT GAGAACATTC 
AGAGTTCTCC GAGCATTGAA AACAATATCA GTCATTCCAG GTCTGAAGAC TATCGTGGGC 
GCACTGATTC AGTCGGTGAA GAAGCTTTCC GATGTAATGA TTCTCACAGT GTTTTGCCTC 
AGTGTGTTTG CACTAATAGG GCTACAGCTT TTCATGGGGC ACCTGAGGAA TAAGTGTGTA 
AGGTGGCCAC CTGAGTATGA ACCAAATATC ACTTCCTACT TTAATGAAAC TGCTGGCCTC 
TATATTAATG TAACTCTGAA CCCATTCAAT CGTGAAGAAT ACATCCTAGA AGAAACTAAT 
TTTTATTTTT TGCCTGGAAC GAGAGATGCT CTTCTGTGTG GCAACAGCTC CGATGCAGGG 
CAGTGTCCAA AAGGATATGT ATGTTTGAAG ACAGGAAGGA ACCCAAACTA CGGCTACACA 
AGTTTTGACA CATTTAGCTG GGCTTTTTTA TCACTGTTTC GGCTAATGAC TCAGGATTTC 
TGGGAAAATC TTTATCAACT GACACTACGA GCTGCTGGTA AAACCTACAT GATATTTTTC 
GTTTTGGTGA TTTTCCTGGG TTCTTTCTAT TTGATAAACT TGATCTTGGC TGTGGTGGCC 
ATGGCTTATG AAGAGCAGAA TCAGGCAACC TTAGAAGAAG CGGAGCAGAA AGAAGCAGAA 
TTCCAGCTTA TGATGGAGCA ACTGAAAAAA CATCAGGAAG AAATTCAGGC AGCGGCGCTA 
GCAGCAGAAA CAATGGACGG TTCCAGAGAG TTTAGTGGTG GGACAGGCTA TGGTGGGCTT 
TCAGAAAGCT CTTCTGAGGC ATCAAAGCCA AGTTCCAAAA GTGCTAAGGA GAGAAGAAAT 
AGAAGGAAAA AGAGAAAACA AAAAGAGCAG GGTGAGGATG AGAAGGCAGA TGATGAAAAA 
TTCCACAAGT CGGCTTCAGA AACGAGTATA AAGAGGAAAG GCTTTCGTTT TTCTATAGAA 
GGAAACCGAC TGACCTATGA GAAGAAGTAC TCGTCCCCAC ATCAGTCCCG CCTCAGTTTC 
CGTGGCTCCC TCTTTTTCCC AAGGCGTGAT AGCAGAGCGA GCCTGTGCAG CTTCAGAGGC 
CGTGGGAAGG ATGTAGGATC AGAAAATGAC TTTGCGGATG ATGAACACAG CACATTTGAG 
GACAATGAAA GCAGAAGAGG TTCATTGTTC GTTCCACACA GGCATGGAGA GAGGAGGAAC 
AGCAACATAA GTCAAACCAG CAAGGCATCA AGGACCCTGC CGGTGCTGCC AGTGAATGGG 
AAGATGCACA GCACGGTGGA TTGCAATGGA GTGGTCTCTC TACTTGGTGG CCCATCAGGT 
CCAACATCGC CTGCTGGACT ACTTCTGCCA GAGGTGATAA TAGATAAACC AGCTTCTGAT 
GACAATGGAA CTAATACAGA GAATGAACTG AGGAAAAGAC GGCTCAGTTC GTATCATGTT 
TCTATGGATT TTCTGGAAGA TCCGATTCTC AGACAAAGGG CTCTGAGCAT TGCCAGCAAT 
CTAACAAATA CAATGGAAGA ACTTGAAGAA TCCAGGGAAA AATGTCCACC ATGCTGGAAT 
AAATTTGCCA ATACATATCT AATCTGGGAT TGCAGCGACT CTTGGCTAAA GATAAAGCAT 
GTTGTCAACT TGATTGTCAT GGACCCATTT GTGGATCTTG CAATAACAAT CTGCATTGTT 
TTGAACACGT TATTTATGGC TATGGAGCAT TACCCAATAA CCGATGAATT TGCCGGTGCG 
CTCAATGTGG GAAATTTGGT GTTTACAGGT ATTTTTACTG CTGAGATGGT TTTCAAGCTA 
ATTGCCCTAG ATCCCTATTA CTATTTCCAA GTTGGCTGGA ACATATTCGA TGGGGTTATT 
GTCAGTCTCA GCTTGATGGA ACTTGGTCTT TCGGATGTGG AAGGGTTATC AGTCCTTCGA 
TCTTTCAGAT TGCTTCGTGT ATTCAAATTG GCGAAATCCT GGCCTACACT GAACATGCTG 
ATAAAAATTA TTGGCAATTC TGTAGGAGCT TTGGGGAACC TGACTTTGGT GTTGGCCATC 
ATTGTCTTCA TTTTTGCTGT GGTTGGGATG CAGCTGTTTG GTAAAAGTTA CAAGGAATGT 
GTCTGCAAAA TTGCCCCAGA TGACTGTGAG CTTCCTCGCT GGCATATGAA CGACTTCTTC 
CACTCCTTCC TTATTGTGTT CCGAGTTCTG TGTGGGGAAT GGATTGAAAC CATGTGGGAC 
TGTATGGAGG TTGCAGGACA AGCTATGTGT CTTACTGTAT TTATGATGGT GATGGTTATT 
GGAAACCTAG TGGTGTTAAA TTTGTTTTTA GCTTTGCTGC TCAGCTCATT TAGCTCAGAC 
AATCTAGCTG CTACAGAGGA CGATAATGAA ATGAACAATC TACAAATTGC AGTGGCCAGA 
ATGCACGAAG GAATAGCCTA TGTGAAAAGG AAGATGCGGG AATTCATACA AAACGCCTTT 
GTCAAAAAGC AAAAAGCCTT AGAAGAAACC AAACCCTTAG AAGACATGCA GAACCAAAAG 
GACAGCTGCA TTTCCAATCA CACCACTGTG GAAATAAGCA AAGAGTATGA CTACCTGAAA 
GATGGAAATG TGACAACGGC CACCAGTGGC ATAGGCAGCA GTGTGGAAAA GTACATCATA 
GACGAAAGCG ACTACATGTC TTTCATTCAT AACCCAAGCC TAACTGTCAC TGTACCTATT 
GCTGTGGGGG AGTCCGATTT TGAGAATCTG AACACAGAGG AGTTTAGCAG CGAATCAGAC 
TTGGAAGAAA GCAAGGAGAA GCTCAACTCA TCCAGCTCAT CAGAAGGCAG TACGGTGGAC 
ATCAGGCCTG CTGGGGAGGG AGAGCCTGTT CCACAGGTGG AGGTTGAAGA GTTCCAGGAA 
CCGGATGCTT GTTTCACAGA TGGCTGTATC AGGAGATTTC CATGCTGTGT GGTTGATGTC 
TCACATGGCA AATGGAAGCA GTGGTGGAAC CTCAGAAAAA CATGTTTCTT AATCGTAGAA 
CACAACTGGT TTGAGACCTT CATAATCTTT ATGATTCTTA TGAGTAGTGG TGCCCTGGCA 
TTTGAAGATA TCTACATAGA ACAAAGAAAA ACAATAAAAA TAATTTTGGA ATATGCGGAC 
AAGGTTTTTA CGTACATCTT TATCTTGGAA ATGCTTCTGA AATGGGTGGC ATATGGCTTT 
CAAAAATATT TCACAAATGC CTGGTGCTGG TTGGACTTCT TGATTGTTGA TGTTTCTTTG 
GTTAGCTTAA TAGCTAATGC CTTGGGCTAC TCAGAATTAG GAGCTATTAA GTCCCTTAGG 
ACACTAAGGG CATTGAGACC TCTGAGGGCC CTCTCACGGT TTGAAGGGAT GAGGGTTGTA 
GTGAATGCCC TCGTTGGAGC TATTCCATCC ATTATGAACG TTCTTCTGGT TTGTTTAATA 
TTTTGGCTCA TTTTTAGCAT TATGGGAGTA AACATGTTTT CCGGAAAGTA TGGCTATTGT 
GTGAATTACA CAGATGACGT AGTGTTTGAC TACACAATAG TTAACAATAG GAGCCAATGT 
ATGGACCTAA TTAGTGAAAA TAAAACGGCC AGATGGAAAA ACGTAAAAGT GAATTTTGAT 
AACGTGGGAT TTGGATATCT TGCTTTGCTT CAAGTGGCAA CATTCAAAGG ATGGATGGAA 
ATAATGTATG CAGCAGTTGA CTCAAGAAAG CAAGAGGAAC AGCCAAAATA TGAAGACAAT 
CTGTACATGT ATTTGTACTT CGTTATTTTC ATCATTTTTG GATCGTTTTT CACACTCAAC 
CTTTTCATTG GTGTCATCAT TGATAATTTT AATCAGCAGA AGAAGAAGTT CGGAGGTCAA 
GACATCTTTA TGACGGAAGA ACAAAAAAAA TATTACAATG CAATGAAGAA GCTGGGGTCC 
AAGAAACCAC AAAAGCCTGT TCCAAGACCT GATAATAAAT TTCAAGGTGC CGTCTTTGAT 
TTTGTGTCGA AACAACCGTT TGATATAGCC ATCATGATTC TGATCTGTTT AAATATGGTA 
ACCATGATGA TTGAAACAGA TGATCAGAGC ATCGAAACGG AGAACAACTT GTACTGGATA 
AATGCAGTTT TCATTGTTCT TTTTACTGGG GAGTGTGTGC TCAAGCTGAT ATCCCTACGT 
CATTATTACT TCACAATTGG CTGGAACATA TTTGATTTTG TGGTTGTAAT TCTCTCTATA 
GTAGGTATGT TTCTGGCAGA CCTTATCGAA AAATATTTTG TGTCACCCAC CCTGTTCAGG 
GTCATCCGAT TAGCTAGGAT TGGTCGTATC CTGCGTCTCA TCAAGGGAGC TAAAGGCATT 
CGTACTCTGC TGTTTGCCCT GATGATGTCA CTTCCTGCGT TGTTCAATAT TGGTTTACTG 
CTCTTTTTGG TGATGTTCAT CTACGCCATT TTCGGCATGT CCAACTTTGC CTATGTTAAG 
AAAGAGGCTG GGATTGATGA CATGTTTAAC TTTGAGACCT TTGGTAACAG CATGATCTGT 
TTATTCCAAA TTACAACATC TGCAGGATGG GATGGTCTGT TAGCGCCAAT TCTTAATAGC 
GGGGCACCAG ATTGCGATCC AAAGGCAGAA CATTCAGGAA GCAGTGTAAA AGGAGACTGT 
GGCAATCCCT CTGTTGGCAT CTTCTTTTTT GTTAGTTATA TCATTATATC ATTCTTGGTT 
GTAGTCAACA TGTATATTGC TGTCATCTTG GAAAACTTCA GTGTTGCTAC GGAGGAAAGT 
GCCGAACCTC TAGGAGAAGA TGATTTTGAG ATGTTCTATG AGGTCTGGGA AAAATTTGAT 
TCAAATGCAA CACAGTTTAT ACAATACGTT AAACTGACTG ATTTTGCTGA TGCGCTGGAT 
CCGCCTCTCC GAATGCCGAA ACCAAACAAT GCGCAGTTAA TCGCAATGGA TCTGCCTATG 
GTAAGTGGGG ACAGAATTCA CTGCCTTGAT GTCCTGTTTG CCTTTACAAA GCGTGTGCTA 
GGTGAAGGCG ATGAAATGGA TAACTTAAGA CAGCCAATGG AAGAACGTTT CATGGCATCC 
AATCCTTCAA AGGCCTCCTA CGAACCAATC ACGTCTACTC TACGTAGGAA ACAAGAGGAG 
TTAGCTGCCA TAGTTATCCA GCGCTGCTAC AGATGCTACG CTTTAAGGAA AGGCATAAAA 
CATGCCTCCT TTATGTACAA CCAGGAAAAG GGCAAAGATG GAGGGAGTCT ACTTACTAGG 
AAAGATATGC TTAGCGATCG ACTAAATGGA AACTCTACAA CAGAGAAAGC AGACATTACC 
CCTTCGACAA CGTCTCCTCC GTCCTATGAC AGTGTTACAA AACCAGATAA AGAAAAACAC 
GAAAAAGACA AAATAGAGAA GGAAGACAGG GGCAAAGATG TCAGGGAGCA TAAAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

scn3a ( XM_031892883.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031748743.1 Xenopus tropicalis sodium channel, voltage gated, type III alpha subunit (scn3a), transcript variant X2, mRNA.

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CloneID	OXa66280
Clone ID Related Accession (Same CDS sequence)	XM_031892883.1
Accession Version	XM_031892883.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6060bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sodium channel protein type 2 subunit alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGC CACTGCTACC CCCACCAGGA CCTGACAGCC TTCGACTCCT CACCAGAGAA 
TCTCTTGCTG TTCTTGAAAA GCGCTTTGCA GAAGAAAAAG CCAGGAAGGC ATCACAGAAG 
GATCGCAGAG ATGATGATGA TGAAAATGGA CCCAAGCCTA ATTCGGACTT GGAAGCTGGC 
AAAAACTTAC CATTTATATA TGGAGACATT CCACAGGGAA TGGTGGCAGT ACCAATGGAA 
GACCTGGACC CATATTACAG CAACCAAAAG ACCTTTATAG TATTGAATAG AGGGAAGGCA 
ATCTTCCGAT TCAGTGCCAC ATCTGCCTTG TACATTTTAA CTCCTTTCAA TCCTCTTAGA 
AGAATATCTA TAAAGATTTT GGTACATTCT TTATTCAGCA TGCTCATTAT GTGCACTATT 
TTGACTAATT GCTTCTTCAT GACCATGAGT AACCCACCAG AATGGACCAA GAATGTTGAA 
TATACCTTCA CTGGAATTTA TACATTTGAA TCACTTGTTA AAATACTTGC CAGAGGTTTT 
TGTTTAGAAA GCTTTACTTT TCTACGGGAT CCATGGAATT GGCTTGATTT CAGCGTCATA 
GTCATGGCGT ATGTAACAGA ATTTGTAAAC CTAGGCAATG TTTCAGCTCT TCGAACTTTC 
AGAGTATTGA GAGCTTTGAA AACTATTTCT GTAATTCCAG GTCTGAAGAC TATCGTGGGC 
GCACTGATTC AGTCGGTGAA GAAGCTTTCC GATGTAATGA TTCTCACAGT GTTTTGCCTC 
AGTGTGTTTG CACTAATAGG GCTACAGCTT TTCATGGGGC ACCTGAGGAA TAAGTGTGTA 
AGGTGGCCAC CTGAGTATGA ACCAAATATC ACTTCCTACT TTAATGAAAC TGCTGGCCTC 
TATATTAATG TAACTCTGAA CCCATTCAAT CGTGAAGAAT ACATCCTAGA AGAAACTAAT 
TTTTATTTTT TGCCTGGAAC GAGAGATGCT CTTCTGTGTG GCAACAGCTC CGATGCAGGG 
CAGTGTCCAA AAGGATATGT ATGTTTGAAG ACAGGAAGGA ACCCAAACTA CGGCTACACA 
AGTTTTGACA CATTTAGCTG GGCTTTTTTA TCACTGTTTC GGCTAATGAC TCAGGATTTC 
TGGGAAAATC TTTATCAACT GACACTACGA GCTGCTGGTA AAACCTACAT GATATTTTTC 
GTTTTGGTGA TTTTCCTGGG TTCTTTCTAT TTGATAAACT TGATCTTGGC TGTGGTGGCC 
ATGGCTTATG AAGAGCAGAA TCAGGCAACC TTAGAAGAAG CGGAGCAGAA AGAAGCAGAA 
TTCCAGCTTA TGATGGAGCA ACTGAAAAAA CATCAGGAAG AAATTCAGGC AGCGGCGCTA 
GCAGCAGAAA CAATGGACGG TTCCAGAGAG TTTAGTGGTG GGACAGGCTA TGGTGGGCTT 
TCAGAAAGCT CTTCTGAGGC ATCAAAGCCA AGTTCCAAAA GTGCTAAGGA GAGAAGAAAT 
AGAAGGAAAA AGAGAAAACA AAAAGAGCAG GGTGAGGATG AGAAGGCAGA TGATGAAAAA 
TTCCACAAGT CGGCTTCAGA AACGAGTATA AAGAGGAAAG GCTTTCGTTT TTCTATAGAA 
GGAAACCGAC TGACCTATGA GAAGAAGTAC TCGTCCCCAC ATCAGTCCCG CCTCAGTTTC 
CGTGGCTCCC TCTTTTTCCC AAGGCGTGAT AGCAGAGCGA GCCTGTGCAG CTTCAGAGGC 
CGTGGGAAGG ATGTAGGATC AGAAAATGAC TTTGCGGATG ATGAACACAG CACATTTGAG 
GACAATGAAA GCAGAAGAGG TTCATTGTTC GTTCCACACA GGCATGGAGA GAGGAGGAAC 
AGCAACATAA GTCAAACCAG CAAGGCATCA AGGACCCTGC CGGTGCTGCC AGTGAATGGG 
AAGATGCACA GCACGGTGGA TTGCAATGGA GTGGTCTCTC TACTTGGTGG CCCATCAGGT 
CCAACATCGC CTGCTGGACT ACTTCTGCCA GAGGTGATAA TAGATAAACC AGCTTCTGAT 
GACAATGGAA CTAATACAGA GAATGAACTG AGGAAAAGAC GGCTCAGTTC GTATCATGTT 
TCTATGGATT TTCTGGAAGA TCCGATTCTC AGACAAAGGG CTCTGAGCAT TGCCAGCAAT 
CTAACAAATA CAATGGAAGA ACTTGAAGAA TCCAGGGAAA AATGTCCACC ATGCTGGAAT 
AAATTTGCCA ATACATATCT AATCTGGGAT TGCAGCGACT CTTGGCTAAA GATAAAGCAT 
GTTGTCAACT TGATTGTCAT GGACCCATTT GTGGATCTTG CAATAACAAT CTGCATTGTT 
TTGAACACGT TATTTATGGC TATGGAGCAT TACCCAATAA CCGATGAATT TGCCGGTGCG 
CTCAATGTGG GAAATTTGGT GTTTACAGGT ATTTTTACTG CTGAGATGGT TTTCAAGCTA 
ATTGCCCTAG ATCCCTATTA CTATTTCCAA GTTGGCTGGA ACATATTCGA TGGGGTTATT 
GTCAGTCTCA GCTTGATGGA ACTTGGTCTT TCGGATGTGG AAGGGTTATC AGTCCTTCGA 
TCTTTCAGAT TGCTTCGTGT ATTCAAATTG GCGAAATCCT GGCCTACACT GAACATGCTG 
ATAAAAATTA TTGGCAATTC TGTAGGAGCT TTGGGGAACC TGACTTTGGT GTTGGCCATC 
ATTGTCTTCA TTTTTGCTGT GGTTGGGATG CAGCTGTTTG GTAAAAGTTA CAAGGAATGT 
GTCTGCAAAA TTGCCCCAGA TGACTGTGAG CTTCCTCGCT GGCATATGAA CGACTTCTTC 
CACTCCTTCC TTATTGTGTT CCGAGTTCTG TGTGGGGAAT GGATTGAAAC CATGTGGGAC 
TGTATGGAGG TTGCAGGACA AGCTATGTGT CTTACTGTAT TTATGATGGT GATGGTTATT 
GGAAACCTAG TGGTGTTAAA TTTGTTTTTA GCTTTGCTGC TCAGCTCATT TAGCTCAGAC 
AATCTAGCTG CTACAGAGGA CGATAATGAA ATGAACAATC TACAAATTGC AGTGGCCAGA 
ATGCACGAAG GAATAGCCTA TGTGAAAAGG AAGATGCGGG AATTCATACA AAACGCCTTT 
GTCAAAAAGC AAAAAGCCTT AGAAGAAACC AAACCCTTAG AAGACATGCA GAACCAAAAG 
GACAGCTGCA TTTCCAATCA CACCACTGTG GAAATAAGCA AAGAGTATGA CTACCTGAAA 
GATGGAAATG TGACAACGGC CACCAGTGGC ATAGGCAGCA GTGTGGAAAA GTACATCATA 
GACGAAAGCG ACTACATGTC TTTCATTCAT AACCCAAGCC TAACTGTCAC TGTACCTATT 
GCTGTGGGGG AGTCCGATTT TGAGAATCTG AACACAGAGG AGTTTAGCAG CGAATCAGAC 
TTGGAAGAAA GCAAGGAGAA GCTCAACTCA TCCAGCTCAT CAGAAGGCAG TACGGTGGAC 
ATCAGGCCTG CTGGGGAGGG AGAGCCTGTT CCACAGGTGG AGGTTGAAGA GTTCCAGGAA 
CCGGATGCTT GTTTCACAGA TGGCTGTATC AGGAGATTTC CATGCTGTGT GGTTGATGTC 
TCACATGGCA AATGGAAGCA GTGGTGGAAC CTCAGAAAAA CATGTTTCTT AATCGTAGAA 
CACAACTGGT TTGAGACCTT CATAATCTTT ATGATTCTTA TGAGTAGTGG TGCCCTGGCA 
TTTGAAGATA TCTACATAGA ACAAAGAAAA ACAATAAAAA TAATTTTGGA ATATGCGGAC 
AAGGTTTTTA CGTACATCTT TATCTTGGAA ATGCTTCTGA AATGGGTGGC ATATGGCTTT 
CAAAAATATT TCACAAATGC CTGGTGCTGG TTGGACTTCT TGATTGTTGA TGTTTCTTTG 
GTTAGCTTAA TAGCTAATGC CTTGGGCTAC TCAGAATTAG GAGCTATTAA GTCCCTTAGG 
ACACTAAGGG CATTGAGACC TCTGAGGGCC CTCTCACGGT TTGAAGGGAT GAGGGTTGTA 
GTGAATGCCC TCGTTGGAGC TATTCCATCC ATTATGAACG TTCTTCTGGT TTGTTTAATA 
TTTTGGCTCA TTTTTAGCAT TATGGGAGTA AACATGTTTT CCGGAAAGTA TGGCTATTGT 
GTGAATTACA CAGATGACGT AGTGTTTGAC TACACAATAG TTAACAATAG GAGCCAATGT 
ATGGACCTAA TTAGTGAAAA TAAAACGGCC AGATGGAAAA ACGTAAAAGT GAATTTTGAT 
AACGTGGGAT TTGGATATCT TGCTTTGCTT CAAGTGGCAA CATTCAAAGG ATGGATGGAA 
ATAATGTATG CAGCAGTTGA CTCAAGAAAG CAAGAGGAAC AGCCAAAATA TGAAGACAAT 
CTGTACATGT ATTTGTACTT CGTTATTTTC ATCATTTTTG GATCGTTTTT CACACTCAAC 
CTTTTCATTG GTGTCATCAT TGATAATTTT AATCAGCAGA AGAAGAAGTT CGGAGGTCAA 
GACATCTTTA TGACGGAAGA ACAAAAAAAA TATTACAATG CAATGAAGAA GCTGGGGTCC 
AAGAAACCAC AAAAGCCTGT TCCAAGACCT GATAATAAAT TTCAAGGTGC CGTCTTTGAT 
TTTGTGTCGA AACAACCGTT TGATATAGCC ATCATGATTC TGATCTGTTT AAATATGGTA 
ACCATGATGA TTGAAACAGA TGATCAGAGC ATCGAAACGG AGAACAACTT GTACTGGATA 
AATGCAGTTT TCATTGTTCT TTTTACTGGG GAGTGTGTGC TCAAGCTGAT ATCCCTACGT 
CATTATTACT TCACAATTGG CTGGAACATA TTTGATTTTG TGGTTGTAAT TCTCTCTATA 
GTAGGTATGT TTCTGGCAGA CCTTATCGAA AAATATTTTG TGTCACCCAC CCTGTTCAGG 
GTCATCCGAT TAGCTAGGAT TGGTCGTATC CTGCGTCTCA TCAAGGGAGC TAAAGGCATT 
CGTACTCTGC TGTTTGCCCT GATGATGTCA CTTCCTGCGT TGTTCAATAT TGGTTTACTG 
CTCTTTTTGG TGATGTTCAT CTACGCCATT TTCGGCATGT CCAACTTTGC CTATGTTAAG 
AAAGAGGCTG GGATTGATGA CATGTTTAAC TTTGAGACCT TTGGTAACAG CATGATCTGT 
TTATTCCAAA TTACAACATC TGCAGGATGG GATGGTCTGT TAGCGCCAAT TCTTAATAGC 
GGGGCACCAG ATTGCGATCC AAAGGCAGAA CATTCAGGAA GCAGTGTAAA AGGAGACTGT 
GGCAATCCCT CTGTTGGCAT CTTCTTTTTT GTTAGTTATA TCATTATATC ATTCTTGGTT 
GTAGTCAACA TGTATATTGC TGTCATCTTG GAAAACTTCA GTGTTGCTAC GGAGGAAAGT 
GCCGAACCTC TAGGAGAAGA TGATTTTGAG ATGTTCTATG AGGTCTGGGA AAAATTTGAT 
TCAAATGCAA CACAGTTTAT ACAATACGTT AAACTGACTG ATTTTGCTGA TGCGCTGGAT 
CCGCCTCTCC GAATGCCGAA ACCAAACAAT GCGCAGTTAA TCGCAATGGA TCTGCCTATG 
GTAAGTGGGG ACAGAATTCA CTGCCTTGAT GTCCTGTTTG CCTTTACAAA GCGTGTGCTA 
GGTGAAGGCG ATGAAATGGA TAACTTAAGA CAGCCAATGG AAGAACGTTT CATGGCATCC 
AATCCTTCAA AGGCCTCCTA CGAACCAATC ACGTCTACTC TACGTAGGAA ACAAGAGGAG 
TTAGCTGCCA TAGTTATCCA GCGCTGCTAC AGATGCTACG CTTTAAGGAA AGGCATAAAA 
CATGCCTCCT TTATGTACAA CCAGGAAAAG GGCAAAGATG GAGGGAGTCT ACTTACTAGG 
AAAGATATGC TTAGCGATCG ACTAAATGGA AACTCTACAA CAGAGAAAGC AGACATTACC 
CCTTCGACAA CGTCTCCTCC GTCCTATGAC AGTGTTACAA AACCAGATAA AGAAAAACAC 
GAAAAAGACA AAATAGAGAA GGAAGACAGG GGCAAAGATG TCAGGGAGCA TAAAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031748743.1
CDS	262..6321
Translation	MEQPLLPPPGPDSLRLLTRESLAVLEKRFAEEKARKASQKDRRDDDDENGPKPNSDLEAGKNLPFIYGDIPQGMVAVPMEDLDPYYSNQKTFIVLNRGKAIFRFSATSALYILTPFNPLRRISIKILVHSLFSMLIMCTILTNCFFMTMSNPPEWTKNVEYTFTGIYTFESLVKILARGFCLESFTFLRDPWNWLDFSVIVMAYVTEFVNLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGHLRNKCVRWPPEYEPNITSYFNETAGLYINVTLNPFNREEYILEETNFYFLPGTRDALLCGNSSDAGQCPKGYVCLKTGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQLMMEQLKKHQEEIQAAALAAETMDGSREFSGGTGYGGLSESSSEASKPSSKSAKERRNRRKKRKQKEQGEDEKADDEKFHKSASETSIKRKGFRFSIEGNRLTYEKKYSSPHQSRLSFRGSLFFPRRDSRASLCSFRGRGKDVGSENDFADDEHSTFEDNESRRGSLFVPHRHGERRNSNISQTSKASRTLPVLPVNGKMHSTVDCNGVVSLLGGPSGPTSPAGLLLPEVIIDKPASDDNGTNTENELRKRRLSSYHVSMDFLEDPILRQRALSIASNLTNTMEELEESREKCPPCWNKFANTYLIWDCSDSWLKIKHVVNLIVMDPFVDLAITICIVLNTLFMAMEHYPITDEFAGALNVGNLVFTGIFTAEMVFKLIALDPYYYFQVGWNIFDGVIVSLSLMELGLSDVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKIAPDDCELPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSSDNLAATEDDNEMNNLQIAVARMHEGIAYVKRKMREFIQNAFVKKQKALEETKPLEDMQNQKDSCISNHTTVEISKEYDYLKDGNVTTATSGIGSSVEKYIIDESDYMSFIHNPSLTVTVPIAVGESDFENLNTEEFSSESDLEESKEKLNSSSSSEGSTVDIRPAGEGEPVPQVEVEEFQEPDACFTDGCIRRFPCCVVDVSHGKWKQWWNLRKTCFLIVEHNWFETFIIFMILMSSGALAFEDIYIEQRKTIKIILEYADKVFTYIFILEMLLKWVAYGFQKYFTNAWCWLDFLIVDVSLVSLIANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNMFSGKYGYCVNYTDDVVFDYTIVNNRSQCMDLISENKTARWKNVKVNFDNVGFGYLALLQVATFKGWMEIMYAAVDSRKQEEQPKYEDNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPVPRPDNKFQGAVFDFVSKQPFDIAIMILICLNMVTMMIETDDQSIETENNLYWINAVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLADLIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKKEAGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSGAPDCDPKAEHSGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLGEDDFEMFYEVWEKFDSNATQFIQYVKLTDFADALDPPLRMPKPNNAQLIAMDLPMVSGDRIHCLDVLFAFTKRVLGEGDEMDNLRQPMEERFMASNPSKASYEPITSTLRRKQEELAAIVIQRCYRCYALRKGIKHASFMYNQEKGKDGGSLLTRKDMLSDRLNGNSTTEKADITPSTTSPPSYDSVTKPDKEKHEKDKIEKEDRGKDVREHKK

Target ORF information:

RefSeq Version	XM_031892883.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sodium channel, voltage gated, type III alpha subunit (scn3a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031892883.1

ORF Insert Sequence:

ATGGAGCAGC CACTGCTACC CCCACCAGGA CCTGACAGCC TTCGACTCCT CACCAGAGAA 
TCTCTTGCTG TTCTTGAAAA GCGCTTTGCA GAAGAAAAAG CCAGGAAGGC ATCACAGAAG 
GATCGCAGAG ATGATGATGA TGAAAATGGA CCCAAGCCTA ATTCGGACTT GGAAGCTGGC 
AAAAACTTAC CATTTATATA TGGAGACATT CCACAGGGAA TGGTGGCAGT ACCAATGGAA 
GACCTGGACC CATATTACAG CAACCAAAAG ACCTTTATAG TATTGAATAG AGGGAAGGCA 
ATCTTCCGAT TCAGTGCCAC ATCTGCCTTG TACATTTTAA CTCCTTTCAA TCCTCTTAGA 
AGAATATCTA TAAAGATTTT GGTACATTCT TTATTCAGCA TGCTCATTAT GTGCACTATT 
TTGACTAATT GCTTCTTCAT GACCATGAGT AACCCACCAG AATGGACCAA GAATGTTGAA 
TATACCTTCA CTGGAATTTA TACATTTGAA TCACTTGTTA AAATACTTGC CAGAGGTTTT 
TGTTTAGAAA GCTTTACTTT TCTACGGGAT CCATGGAATT GGCTTGATTT CAGCGTCATA 
GTCATGGCGT ATGTAACAGA ATTTGTAAAC CTAGGCAATG TTTCAGCTCT TCGAACTTTC 
AGAGTATTGA GAGCTTTGAA AACTATTTCT GTAATTCCAG GTCTGAAGAC TATCGTGGGC 
GCACTGATTC AGTCGGTGAA GAAGCTTTCC GATGTAATGA TTCTCACAGT GTTTTGCCTC 
AGTGTGTTTG CACTAATAGG GCTACAGCTT TTCATGGGGC ACCTGAGGAA TAAGTGTGTA 
AGGTGGCCAC CTGAGTATGA ACCAAATATC ACTTCCTACT TTAATGAAAC TGCTGGCCTC 
TATATTAATG TAACTCTGAA CCCATTCAAT CGTGAAGAAT ACATCCTAGA AGAAACTAAT 
TTTTATTTTT TGCCTGGAAC GAGAGATGCT CTTCTGTGTG GCAACAGCTC CGATGCAGGG 
CAGTGTCCAA AAGGATATGT ATGTTTGAAG ACAGGAAGGA ACCCAAACTA CGGCTACACA 
AGTTTTGACA CATTTAGCTG GGCTTTTTTA TCACTGTTTC GGCTAATGAC TCAGGATTTC 
TGGGAAAATC TTTATCAACT GACACTACGA GCTGCTGGTA AAACCTACAT GATATTTTTC 
GTTTTGGTGA TTTTCCTGGG TTCTTTCTAT TTGATAAACT TGATCTTGGC TGTGGTGGCC 
ATGGCTTATG AAGAGCAGAA TCAGGCAACC TTAGAAGAAG CGGAGCAGAA AGAAGCAGAA 
TTCCAGCTTA TGATGGAGCA ACTGAAAAAA CATCAGGAAG AAATTCAGGC AGCGGCGCTA 
GCAGCAGAAA CAATGGACGG TTCCAGAGAG TTTAGTGGTG GGACAGGCTA TGGTGGGCTT 
TCAGAAAGCT CTTCTGAGGC ATCAAAGCCA AGTTCCAAAA GTGCTAAGGA GAGAAGAAAT 
AGAAGGAAAA AGAGAAAACA AAAAGAGCAG GGTGAGGATG AGAAGGCAGA TGATGAAAAA 
TTCCACAAGT CGGCTTCAGA AACGAGTATA AAGAGGAAAG GCTTTCGTTT TTCTATAGAA 
GGAAACCGAC TGACCTATGA GAAGAAGTAC TCGTCCCCAC ATCAGTCCCG CCTCAGTTTC 
CGTGGCTCCC TCTTTTTCCC AAGGCGTGAT AGCAGAGCGA GCCTGTGCAG CTTCAGAGGC 
CGTGGGAAGG ATGTAGGATC AGAAAATGAC TTTGCGGATG ATGAACACAG CACATTTGAG 
GACAATGAAA GCAGAAGAGG TTCATTGTTC GTTCCACACA GGCATGGAGA GAGGAGGAAC 
AGCAACATAA GTCAAACCAG CAAGGCATCA AGGACCCTGC CGGTGCTGCC AGTGAATGGG 
AAGATGCACA GCACGGTGGA TTGCAATGGA GTGGTCTCTC TACTTGGTGG CCCATCAGGT 
CCAACATCGC CTGCTGGACT ACTTCTGCCA GAGGTGATAA TAGATAAACC AGCTTCTGAT 
GACAATGGAA CTAATACAGA GAATGAACTG AGGAAAAGAC GGCTCAGTTC GTATCATGTT 
TCTATGGATT TTCTGGAAGA TCCGATTCTC AGACAAAGGG CTCTGAGCAT TGCCAGCAAT 
CTAACAAATA CAATGGAAGA ACTTGAAGAA TCCAGGGAAA AATGTCCACC ATGCTGGAAT 
AAATTTGCCA ATACATATCT AATCTGGGAT TGCAGCGACT CTTGGCTAAA GATAAAGCAT 
GTTGTCAACT TGATTGTCAT GGACCCATTT GTGGATCTTG CAATAACAAT CTGCATTGTT 
TTGAACACGT TATTTATGGC TATGGAGCAT TACCCAATAA CCGATGAATT TGCCGGTGCG 
CTCAATGTGG GAAATTTGGT GTTTACAGGT ATTTTTACTG CTGAGATGGT TTTCAAGCTA 
ATTGCCCTAG ATCCCTATTA CTATTTCCAA GTTGGCTGGA ACATATTCGA TGGGGTTATT 
GTCAGTCTCA GCTTGATGGA ACTTGGTCTT TCGGATGTGG AAGGGTTATC AGTCCTTCGA 
TCTTTCAGAT TGCTTCGTGT ATTCAAATTG GCGAAATCCT GGCCTACACT GAACATGCTG 
ATAAAAATTA TTGGCAATTC TGTAGGAGCT TTGGGGAACC TGACTTTGGT GTTGGCCATC 
ATTGTCTTCA TTTTTGCTGT GGTTGGGATG CAGCTGTTTG GTAAAAGTTA CAAGGAATGT 
GTCTGCAAAA TTGCCCCAGA TGACTGTGAG CTTCCTCGCT GGCATATGAA CGACTTCTTC 
CACTCCTTCC TTATTGTGTT CCGAGTTCTG TGTGGGGAAT GGATTGAAAC CATGTGGGAC 
TGTATGGAGG TTGCAGGACA AGCTATGTGT CTTACTGTAT TTATGATGGT GATGGTTATT 
GGAAACCTAG TGGTGTTAAA TTTGTTTTTA GCTTTGCTGC TCAGCTCATT TAGCTCAGAC 
AATCTAGCTG CTACAGAGGA CGATAATGAA ATGAACAATC TACAAATTGC AGTGGCCAGA 
ATGCACGAAG GAATAGCCTA TGTGAAAAGG AAGATGCGGG AATTCATACA AAACGCCTTT 
GTCAAAAAGC AAAAAGCCTT AGAAGAAACC AAACCCTTAG AAGACATGCA GAACCAAAAG 
GACAGCTGCA TTTCCAATCA CACCACTGTG GAAATAAGCA AAGAGTATGA CTACCTGAAA 
GATGGAAATG TGACAACGGC CACCAGTGGC ATAGGCAGCA GTGTGGAAAA GTACATCATA 
GACGAAAGCG ACTACATGTC TTTCATTCAT AACCCAAGCC TAACTGTCAC TGTACCTATT 
GCTGTGGGGG AGTCCGATTT TGAGAATCTG AACACAGAGG AGTTTAGCAG CGAATCAGAC 
TTGGAAGAAA GCAAGGAGAA GCTCAACTCA TCCAGCTCAT CAGAAGGCAG TACGGTGGAC 
ATCAGGCCTG CTGGGGAGGG AGAGCCTGTT CCACAGGTGG AGGTTGAAGA GTTCCAGGAA 
CCGGATGCTT GTTTCACAGA TGGCTGTATC AGGAGATTTC CATGCTGTGT GGTTGATGTC 
TCACATGGCA AATGGAAGCA GTGGTGGAAC CTCAGAAAAA CATGTTTCTT AATCGTAGAA 
CACAACTGGT TTGAGACCTT CATAATCTTT ATGATTCTTA TGAGTAGTGG TGCCCTGGCA 
TTTGAAGATA TCTACATAGA ACAAAGAAAA ACAATAAAAA TAATTTTGGA ATATGCGGAC 
AAGGTTTTTA CGTACATCTT TATCTTGGAA ATGCTTCTGA AATGGGTGGC ATATGGCTTT 
CAAAAATATT TCACAAATGC CTGGTGCTGG TTGGACTTCT TGATTGTTGA TGTTTCTTTG 
GTTAGCTTAA TAGCTAATGC CTTGGGCTAC TCAGAATTAG GAGCTATTAA GTCCCTTAGG 
ACACTAAGGG CATTGAGACC TCTGAGGGCC CTCTCACGGT TTGAAGGGAT GAGGGTTGTA 
GTGAATGCCC TCGTTGGAGC TATTCCATCC ATTATGAACG TTCTTCTGGT TTGTTTAATA 
TTTTGGCTCA TTTTTAGCAT TATGGGAGTA AACATGTTTT CCGGAAAGTA TGGCTATTGT 
GTGAATTACA CAGATGACGT AGTGTTTGAC TACACAATAG TTAACAATAG GAGCCAATGT 
ATGGACCTAA TTAGTGAAAA TAAAACGGCC AGATGGAAAA ACGTAAAAGT GAATTTTGAT 
AACGTGGGAT TTGGATATCT TGCTTTGCTT CAAGTGGCAA CATTCAAAGG ATGGATGGAA 
ATAATGTATG CAGCAGTTGA CTCAAGAAAG CAAGAGGAAC AGCCAAAATA TGAAGACAAT 
CTGTACATGT ATTTGTACTT CGTTATTTTC ATCATTTTTG GATCGTTTTT CACACTCAAC 
CTTTTCATTG GTGTCATCAT TGATAATTTT AATCAGCAGA AGAAGAAGTT CGGAGGTCAA 
GACATCTTTA TGACGGAAGA ACAAAAAAAA TATTACAATG CAATGAAGAA GCTGGGGTCC 
AAGAAACCAC AAAAGCCTGT TCCAAGACCT GATAATAAAT TTCAAGGTGC CGTCTTTGAT 
TTTGTGTCGA AACAACCGTT TGATATAGCC ATCATGATTC TGATCTGTTT AAATATGGTA 
ACCATGATGA TTGAAACAGA TGATCAGAGC ATCGAAACGG AGAACAACTT GTACTGGATA 
AATGCAGTTT TCATTGTTCT TTTTACTGGG GAGTGTGTGC TCAAGCTGAT ATCCCTACGT 
CATTATTACT TCACAATTGG CTGGAACATA TTTGATTTTG TGGTTGTAAT TCTCTCTATA 
GTAGGTATGT TTCTGGCAGA CCTTATCGAA AAATATTTTG TGTCACCCAC CCTGTTCAGG 
GTCATCCGAT TAGCTAGGAT TGGTCGTATC CTGCGTCTCA TCAAGGGAGC TAAAGGCATT 
CGTACTCTGC TGTTTGCCCT GATGATGTCA CTTCCTGCGT TGTTCAATAT TGGTTTACTG 
CTCTTTTTGG TGATGTTCAT CTACGCCATT TTCGGCATGT CCAACTTTGC CTATGTTAAG 
AAAGAGGCTG GGATTGATGA CATGTTTAAC TTTGAGACCT TTGGTAACAG CATGATCTGT 
TTATTCCAAA TTACAACATC TGCAGGATGG GATGGTCTGT TAGCGCCAAT TCTTAATAGC 
GGGGCACCAG ATTGCGATCC AAAGGCAGAA CATTCAGGAA GCAGTGTAAA AGGAGACTGT 
GGCAATCCCT CTGTTGGCAT CTTCTTTTTT GTTAGTTATA TCATTATATC ATTCTTGGTT 
GTAGTCAACA TGTATATTGC TGTCATCTTG GAAAACTTCA GTGTTGCTAC GGAGGAAAGT 
GCCGAACCTC TAGGAGAAGA TGATTTTGAG ATGTTCTATG AGGTCTGGGA AAAATTTGAT 
TCAAATGCAA CACAGTTTAT ACAATACGTT AAACTGACTG ATTTTGCTGA TGCGCTGGAT 
CCGCCTCTCC GAATGCCGAA ACCAAACAAT GCGCAGTTAA TCGCAATGGA TCTGCCTATG 
GTAAGTGGGG ACAGAATTCA CTGCCTTGAT GTCCTGTTTG CCTTTACAAA GCGTGTGCTA 
GGTGAAGGCG ATGAAATGGA TAACTTAAGA CAGCCAATGG AAGAACGTTT CATGGCATCC 
AATCCTTCAA AGGCCTCCTA CGAACCAATC ACGTCTACTC TACGTAGGAA ACAAGAGGAG 
TTAGCTGCCA TAGTTATCCA GCGCTGCTAC AGATGCTACG CTTTAAGGAA AGGCATAAAA 
CATGCCTCCT TTATGTACAA CCAGGAAAAG GGCAAAGATG GAGGGAGTCT ACTTACTAGG 
AAAGATATGC TTAGCGATCG ACTAAATGGA AACTCTACAA CAGAGAAAGC AGACATTACC 
CCTTCGACAA CGTCTCCTCC GTCCTATGAC AGTGTTACAA AACCAGATAA AGAAAAACAC 
GAAAAAGACA AAATAGAGAA GGAAGACAGG GGCAAAGATG TCAGGGAGCA TAAAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

scn3a ( XM_031892884.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031748744.1 Xenopus tropicalis sodium channel, voltage gated, type III alpha subunit (scn3a), transcript variant X3, mRNA.

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CloneID	OXa66281
Clone ID Related Accession (Same CDS sequence)	XM_031892884.1
Accession Version	XM_031892884.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6060bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sodium channel protein type 2 subunit alpha isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGC CACTGCTACC CCCACCAGGA CCTGACAGCC TTCGACTCCT CACCAGAGAA 
TCTCTTGCTG TTCTTGAAAA GCGCTTTGCA GAAGAAAAAG CCAGGAAGGC ATCACAGAAG 
GATCGCAGAG ATGATGATGA TGAAAATGGA CCCAAGCCTA ATTCGGACTT GGAAGCTGGC 
AAAAACTTAC CATTTATATA TGGAGACATT CCACAGGGAA TGGTGGCAGT ACCAATGGAA 
GACCTGGACC CATATTACAG CAACCAAAAG ACCTTTATAG TATTGAATAG AGGGAAGGCA 
ATCTTCCGAT TCAGTGCCAC ATCTGCCTTG TACATTTTAA CTCCTTTCAA TCCTCTTAGA 
AGAATATCTA TAAAGATTTT GGTACATTCT TTATTCAGCA TGCTCATTAT GTGCACTATT 
TTGACTAATT GCTTCTTCAT GACCATGAGT AACCCACCAG AATGGACCAA GAATGTTGAA 
TATACCTTCA CTGGAATTTA TACATTTGAA TCACTTGTTA AAATACTTGC CAGAGGTTTT 
TGTTTAGAAA GCTTTACTTT TCTACGGGAT CCATGGAATT GGCTTGATTT CAGCGTCATA 
GTCATGGCAT ATGTGACAGA GTTTGTGGAC CTGGGCAATG TCTCAGCATT GAGAACATTC 
AGAGTTCTCC GAGCATTGAA AACAATATCA GTCATTCCAG GTCTGAAGAC TATCGTGGGC 
GCACTGATTC AGTCGGTGAA GAAGCTTTCC GATGTAATGA TTCTCACAGT GTTTTGCCTC 
AGTGTGTTTG CACTAATAGG GCTACAGCTT TTCATGGGGC ACCTGAGGAA TAAGTGTGTA 
AGGTGGCCAC CTGAGTATGA ACCAAATATC ACTTCCTACT TTAATGAAAC TGCTGGCCTC 
TATATTAATG TAACTCTGAA CCCATTCAAT CGTGAAGAAT ACATCCTAGA AGAAACTAAT 
TTTTATTTTT TGCCTGGAAC GAGAGATGCT CTTCTGTGTG GCAACAGCTC CGATGCAGGG 
CAGTGTCCAA AAGGATATGT ATGTTTGAAG ACAGGAAGGA ACCCAAACTA CGGCTACACA 
AGTTTTGACA CATTTAGCTG GGCTTTTTTA TCACTGTTTC GGCTAATGAC TCAGGATTTC 
TGGGAAAATC TTTATCAACT GACACTACGA GCTGCTGGTA AAACCTACAT GATATTTTTC 
GTTTTGGTGA TTTTCCTGGG TTCTTTCTAT TTGATAAACT TGATCTTGGC TGTGGTGGCC 
ATGGCTTATG AAGAGCAGAA TCAGGCAACC TTAGAAGAAG CGGAGCAGAA AGAAGCAGAA 
TTCCAGCTTA TGATGGAGCA ACTGAAAAAA CATCAGGAAG AAATTCAGGC AGCGGCGCTA 
GCAGCAGAAA CAATGGACGG TTCCAGAGAG TTTAGTGGTG GGACAGGCTA TGGTGGGCTT 
TCAGAAAGCT CTTCTGAGGC ATCAAAGCCA AGTTCCAAAA GTGCTAAGGA GAGAAGAAAT 
AGAAGGAAAA AGAGAAAACA AAAAGAGCAG GGTGAGGATG AGAAGGCAGA TGATGAAAAA 
TTCCACAAGT CGGCTTCAGA AACGAGTATA AAGAGGAAAG GCTTTCGTTT TTCTATAGAA 
GGAAACCGAC TGACCTATGA GAAGAAGTAC TCGTCCCCAC ATCAGTCCCG CCTCAGTTTC 
CGTGGCTCCC TCTTTTTCCC AAGGCGTGAT AGCAGAGCGA GCCTGTGCAG CTTCAGAGGC 
CGTGGGAAGG ATGTAGGATC AGAAAATGAC TTTGCGGATG ATGAACACAG CACATTTGAG 
GACAATGAAA GCAGAAGAGG TTCATTGTTC GTTCCACACA GGCATGGAGA GAGGAGGAAC 
AGCAACATAA GTCAAACCAG CAAGGCATCA AGGACCCTGC CGGTGCTGCC AGTGAATGGG 
AAGATGCACA GCACGGTGGA TTGCAATGGA GTGGTCTCTC TACTTGGTGG CCCATCAGGT 
CCAACATCGC CTGCTGGACT ACTTCTGCCA GAGGTGATAA TAGATAAACC AGCTTCTGAT 
GACAATGGAA CTAATACAGA GAATGAACTG AGGAAAAGAC GGCTCAGTTC GTATCATGTT 
TCTATGGATT TTCTGGAAGA TCCGATTCTC AGACAAAGGG CTCTGAGCAT TGCCAGCAAT 
CTAACAAATA CAATGGAAGA ACTTGAAGAA TCCAGGGAAA AATGTCCACC ATGCTGGAAT 
AAATTTGCCA ATACATATCT AATCTGGGAT TGCAGCGACT CTTGGCTAAA GATAAAGCAT 
GTTGTCAACT TGATTGTCAT GGACCCATTT GTGGATCTTG CAATAACAAT CTGCATTGTT 
TTGAACACGT TATTTATGGC TATGGAGCAT TACCCAATAA CCGATGAATT TGCCGGTGCG 
CTCAATGTGG GAAATTTGGT GTTTACAGGT ATTTTTACTG CTGAGATGGT TTTCAAGCTA 
ATTGCCCTAG ATCCCTATTA CTATTTCCAA GTTGGCTGGA ACATATTCGA TGGGGTTATT 
GTCAGTCTCA GCTTGATGGA ACTTGGTCTT TCGGATGTGG AAGGGTTATC AGTCCTTCGA 
TCTTTCAGAT TGCTTCGTGT ATTCAAATTG GCGAAATCCT GGCCTACACT GAACATGCTG 
ATAAAAATTA TTGGCAATTC TGTAGGAGCT TTGGGGAACC TGACTTTGGT GTTGGCCATC 
ATTGTCTTCA TTTTTGCTGT GGTTGGGATG CAGCTGTTTG GTAAAAGTTA CAAGGAATGT 
GTCTGCAAAA TTGCCCCAGA TGACTGTGAG CTTCCTCGCT GGCATATGAA CGACTTCTTC 
CACTCCTTCC TTATTGTGTT CCGAGTTCTG TGTGGGGAAT GGATTGAAAC CATGTGGGAC 
TGTATGGAGG TTGCAGGACA AGCTATGTGT CTTACTGTAT TTATGATGGT GATGGTTATT 
GGAAACCTAG TGGTGTTAAA TTTGTTTTTA GCTTTGCTGC TCAGCTCATT TAGCTCAGAC 
AATCTAGCTG CTACAGAGGA CGATAATGAA ATGAACAATC TACAAATTGC AGTGGCCAGA 
ATGCACGAAG GAATAGCCTA TGTGAAAAGG AAGATGCGGG AATTCATACA AAACGCCTTT 
GTCAAAAAGC AAAAAGCCTT AGAAGAAACC AAACCCTTAG AAGACATGCA GAACCAAAAG 
GACAGCTGCA TTTCCAATCA CACCACTGTG GAAATAAGCA AAGAGTATGA CTACCTGAAA 
GATGGAAATG TGACAACGGC CACCAGTGGC ATAGGCAGCA GTGTGGAAAA GTACATCATA 
GACGAAAGCG ACTACATGTC TTTCATTCAT AACCCAAGCC TAACTGTCAC TGTACCTATT 
GCTGTGGGGG AGTCCGATTT TGAGAATCTG AACACAGAGG AGTTTAGCAG CGAATCAGAC 
TTGGAAGAAA GCAAGGAGAA GCTCAACTCA TCCAGCTCAT CAGAAGGCAG TACGGTGGAC 
ATCAGGCCTG CTGGGGAGGG AGAGCCTGTT CCACAGGTGG AGGTTGAAGA GTTCCAGGAA 
CCGGATGCTT GTTTCACAGA TGGCTGTATC AGGAGATTTC CATGCTGTGT GGTTGATGTC 
TCACATGGCA AATGGAAGCA GTGGTGGAAC CTCAGAAAAA CATGTTTCTT AATCGTAGAA 
CACAACTGGT TTGAGACCTT CATAATCTTT ATGATTCTTA TGAGTAGTGG TGCCCTGGCA 
TTTGAAGATA TCTACATAGA ACAAAGAAAA ACAATAAAAA TAATTTTGGA ATATGCGGAC 
AAGGTTTTTA CGTACATCTT TATCTTGGAA ATGCTTCTGA AATGGGTGGC ATATGGCTTT 
CAAAAATATT TCACAAATGC CTGGTGCTGG TTGGACTTCT TGATTGTTGA TATTTCAATC 
ATATCCCTTT TGGCGGTGGC TCTTGGGTAT GATCAACTAG CCGCCATTAA ATCTCTAAGG 
ACCTTGAGAG CACTGAGACC TCTGCGTGCA TTATCAAGAT TTGAAGGAAT AAGGGTTGTA 
GTGAATGCCC TCGTTGGAGC TATTCCATCC ATTATGAACG TTCTTCTGGT TTGTTTAATA 
TTTTGGCTCA TTTTTAGCAT TATGGGAGTA AACATGTTTT CCGGAAAGTA TGGCTATTGT 
GTGAATTACA CAGATGACGT AGTGTTTGAC TACACAATAG TTAACAATAG GAGCCAATGT 
ATGGACCTAA TTAGTGAAAA TAAAACGGCC AGATGGAAAA ACGTAAAAGT GAATTTTGAT 
AACGTGGGAT TTGGATATCT TGCTTTGCTT CAAGTGGCAA CATTCAAAGG ATGGATGGAA 
ATAATGTATG CAGCAGTTGA CTCAAGAAAG CAAGAGGAAC AGCCAAAATA TGAAGACAAT 
CTGTACATGT ATTTGTACTT CGTTATTTTC ATCATTTTTG GATCGTTTTT CACACTCAAC 
CTTTTCATTG GTGTCATCAT TGATAATTTT AATCAGCAGA AGAAGAAGTT CGGAGGTCAA 
GACATCTTTA TGACGGAAGA ACAAAAAAAA TATTACAATG CAATGAAGAA GCTGGGGTCC 
AAGAAACCAC AAAAGCCTGT TCCAAGACCT GATAATAAAT TTCAAGGTGC CGTCTTTGAT 
TTTGTGTCGA AACAACCGTT TGATATAGCC ATCATGATTC TGATCTGTTT AAATATGGTA 
ACCATGATGA TTGAAACAGA TGATCAGAGC ATCGAAACGG AGAACAACTT GTACTGGATA 
AATGCAGTTT TCATTGTTCT TTTTACTGGG GAGTGTGTGC TCAAGCTGAT ATCCCTACGT 
CATTATTACT TCACAATTGG CTGGAACATA TTTGATTTTG TGGTTGTAAT TCTCTCTATA 
GTAGGTATGT TTCTGGCAGA CCTTATCGAA AAATATTTTG TGTCACCCAC CCTGTTCAGG 
GTCATCCGAT TAGCTAGGAT TGGTCGTATC CTGCGTCTCA TCAAGGGAGC TAAAGGCATT 
CGTACTCTGC TGTTTGCCCT GATGATGTCA CTTCCTGCGT TGTTCAATAT TGGTTTACTG 
CTCTTTTTGG TGATGTTCAT CTACGCCATT TTCGGCATGT CCAACTTTGC CTATGTTAAG 
AAAGAGGCTG GGATTGATGA CATGTTTAAC TTTGAGACCT TTGGTAACAG CATGATCTGT 
TTATTCCAAA TTACAACATC TGCAGGATGG GATGGTCTGT TAGCGCCAAT TCTTAATAGC 
GGGGCACCAG ATTGCGATCC AAAGGCAGAA CATTCAGGAA GCAGTGTAAA AGGAGACTGT 
GGCAATCCCT CTGTTGGCAT CTTCTTTTTT GTTAGTTATA TCATTATATC ATTCTTGGTT 
GTAGTCAACA TGTATATTGC TGTCATCTTG GAAAACTTCA GTGTTGCTAC GGAGGAAAGT 
GCCGAACCTC TAGGAGAAGA TGATTTTGAG ATGTTCTATG AGGTCTGGGA AAAATTTGAT 
TCAAATGCAA CACAGTTTAT ACAATACGTT AAACTGACTG ATTTTGCTGA TGCGCTGGAT 
CCGCCTCTCC GAATGCCGAA ACCAAACAAT GCGCAGTTAA TCGCAATGGA TCTGCCTATG 
GTAAGTGGGG ACAGAATTCA CTGCCTTGAT GTCCTGTTTG CCTTTACAAA GCGTGTGCTA 
GGTGAAGGCG ATGAAATGGA TAACTTAAGA CAGCCAATGG AAGAACGTTT CATGGCATCC 
AATCCTTCAA AGGCCTCCTA CGAACCAATC ACGTCTACTC TACGTAGGAA ACAAGAGGAG 
TTAGCTGCCA TAGTTATCCA GCGCTGCTAC AGATGCTACG CTTTAAGGAA AGGCATAAAA 
CATGCCTCCT TTATGTACAA CCAGGAAAAG GGCAAAGATG GAGGGAGTCT ACTTACTAGG 
AAAGATATGC TTAGCGATCG ACTAAATGGA AACTCTACAA CAGAGAAAGC AGACATTACC 
CCTTCGACAA CGTCTCCTCC GTCCTATGAC AGTGTTACAA AACCAGATAA AGAAAAACAC 
GAAAAAGACA AAATAGAGAA GGAAGACAGG GGCAAAGATG TCAGGGAGCA TAAAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031748744.1
CDS	285..6344
Translation	MEQPLLPPPGPDSLRLLTRESLAVLEKRFAEEKARKASQKDRRDDDDENGPKPNSDLEAGKNLPFIYGDIPQGMVAVPMEDLDPYYSNQKTFIVLNRGKAIFRFSATSALYILTPFNPLRRISIKILVHSLFSMLIMCTILTNCFFMTMSNPPEWTKNVEYTFTGIYTFESLVKILARGFCLESFTFLRDPWNWLDFSVIVMAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGHLRNKCVRWPPEYEPNITSYFNETAGLYINVTLNPFNREEYILEETNFYFLPGTRDALLCGNSSDAGQCPKGYVCLKTGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQLMMEQLKKHQEEIQAAALAAETMDGSREFSGGTGYGGLSESSSEASKPSSKSAKERRNRRKKRKQKEQGEDEKADDEKFHKSASETSIKRKGFRFSIEGNRLTYEKKYSSPHQSRLSFRGSLFFPRRDSRASLCSFRGRGKDVGSENDFADDEHSTFEDNESRRGSLFVPHRHGERRNSNISQTSKASRTLPVLPVNGKMHSTVDCNGVVSLLGGPSGPTSPAGLLLPEVIIDKPASDDNGTNTENELRKRRLSSYHVSMDFLEDPILRQRALSIASNLTNTMEELEESREKCPPCWNKFANTYLIWDCSDSWLKIKHVVNLIVMDPFVDLAITICIVLNTLFMAMEHYPITDEFAGALNVGNLVFTGIFTAEMVFKLIALDPYYYFQVGWNIFDGVIVSLSLMELGLSDVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKIAPDDCELPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSSDNLAATEDDNEMNNLQIAVARMHEGIAYVKRKMREFIQNAFVKKQKALEETKPLEDMQNQKDSCISNHTTVEISKEYDYLKDGNVTTATSGIGSSVEKYIIDESDYMSFIHNPSLTVTVPIAVGESDFENLNTEEFSSESDLEESKEKLNSSSSSEGSTVDIRPAGEGEPVPQVEVEEFQEPDACFTDGCIRRFPCCVVDVSHGKWKQWWNLRKTCFLIVEHNWFETFIIFMILMSSGALAFEDIYIEQRKTIKIILEYADKVFTYIFILEMLLKWVAYGFQKYFTNAWCWLDFLIVDISIISLLAVALGYDQLAAIKSLRTLRALRPLRALSRFEGIRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNMFSGKYGYCVNYTDDVVFDYTIVNNRSQCMDLISENKTARWKNVKVNFDNVGFGYLALLQVATFKGWMEIMYAAVDSRKQEEQPKYEDNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPVPRPDNKFQGAVFDFVSKQPFDIAIMILICLNMVTMMIETDDQSIETENNLYWINAVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLADLIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKKEAGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSGAPDCDPKAEHSGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLGEDDFEMFYEVWEKFDSNATQFIQYVKLTDFADALDPPLRMPKPNNAQLIAMDLPMVSGDRIHCLDVLFAFTKRVLGEGDEMDNLRQPMEERFMASNPSKASYEPITSTLRRKQEELAAIVIQRCYRCYALRKGIKHASFMYNQEKGKDGGSLLTRKDMLSDRLNGNSTTEKADITPSTTSPPSYDSVTKPDKEKHEKDKIEKEDRGKDVREHKK

Target ORF information:

RefSeq Version	XM_031892884.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sodium channel, voltage gated, type III alpha subunit (scn3a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031892884.1

ORF Insert Sequence:

ATGGAGCAGC CACTGCTACC CCCACCAGGA CCTGACAGCC TTCGACTCCT CACCAGAGAA 
TCTCTTGCTG TTCTTGAAAA GCGCTTTGCA GAAGAAAAAG CCAGGAAGGC ATCACAGAAG 
GATCGCAGAG ATGATGATGA TGAAAATGGA CCCAAGCCTA ATTCGGACTT GGAAGCTGGC 
AAAAACTTAC CATTTATATA TGGAGACATT CCACAGGGAA TGGTGGCAGT ACCAATGGAA 
GACCTGGACC CATATTACAG CAACCAAAAG ACCTTTATAG TATTGAATAG AGGGAAGGCA 
ATCTTCCGAT TCAGTGCCAC ATCTGCCTTG TACATTTTAA CTCCTTTCAA TCCTCTTAGA 
AGAATATCTA TAAAGATTTT GGTACATTCT TTATTCAGCA TGCTCATTAT GTGCACTATT 
TTGACTAATT GCTTCTTCAT GACCATGAGT AACCCACCAG AATGGACCAA GAATGTTGAA 
TATACCTTCA CTGGAATTTA TACATTTGAA TCACTTGTTA AAATACTTGC CAGAGGTTTT 
TGTTTAGAAA GCTTTACTTT TCTACGGGAT CCATGGAATT GGCTTGATTT CAGCGTCATA 
GTCATGGCAT ATGTGACAGA GTTTGTGGAC CTGGGCAATG TCTCAGCATT GAGAACATTC 
AGAGTTCTCC GAGCATTGAA AACAATATCA GTCATTCCAG GTCTGAAGAC TATCGTGGGC 
GCACTGATTC AGTCGGTGAA GAAGCTTTCC GATGTAATGA TTCTCACAGT GTTTTGCCTC 
AGTGTGTTTG CACTAATAGG GCTACAGCTT TTCATGGGGC ACCTGAGGAA TAAGTGTGTA 
AGGTGGCCAC CTGAGTATGA ACCAAATATC ACTTCCTACT TTAATGAAAC TGCTGGCCTC 
TATATTAATG TAACTCTGAA CCCATTCAAT CGTGAAGAAT ACATCCTAGA AGAAACTAAT 
TTTTATTTTT TGCCTGGAAC GAGAGATGCT CTTCTGTGTG GCAACAGCTC CGATGCAGGG 
CAGTGTCCAA AAGGATATGT ATGTTTGAAG ACAGGAAGGA ACCCAAACTA CGGCTACACA 
AGTTTTGACA CATTTAGCTG GGCTTTTTTA TCACTGTTTC GGCTAATGAC TCAGGATTTC 
TGGGAAAATC TTTATCAACT GACACTACGA GCTGCTGGTA AAACCTACAT GATATTTTTC 
GTTTTGGTGA TTTTCCTGGG TTCTTTCTAT TTGATAAACT TGATCTTGGC TGTGGTGGCC 
ATGGCTTATG AAGAGCAGAA TCAGGCAACC TTAGAAGAAG CGGAGCAGAA AGAAGCAGAA 
TTCCAGCTTA TGATGGAGCA ACTGAAAAAA CATCAGGAAG AAATTCAGGC AGCGGCGCTA 
GCAGCAGAAA CAATGGACGG TTCCAGAGAG TTTAGTGGTG GGACAGGCTA TGGTGGGCTT 
TCAGAAAGCT CTTCTGAGGC ATCAAAGCCA AGTTCCAAAA GTGCTAAGGA GAGAAGAAAT 
AGAAGGAAAA AGAGAAAACA AAAAGAGCAG GGTGAGGATG AGAAGGCAGA TGATGAAAAA 
TTCCACAAGT CGGCTTCAGA AACGAGTATA AAGAGGAAAG GCTTTCGTTT TTCTATAGAA 
GGAAACCGAC TGACCTATGA GAAGAAGTAC TCGTCCCCAC ATCAGTCCCG CCTCAGTTTC 
CGTGGCTCCC TCTTTTTCCC AAGGCGTGAT AGCAGAGCGA GCCTGTGCAG CTTCAGAGGC 
CGTGGGAAGG ATGTAGGATC AGAAAATGAC TTTGCGGATG ATGAACACAG CACATTTGAG 
GACAATGAAA GCAGAAGAGG TTCATTGTTC GTTCCACACA GGCATGGAGA GAGGAGGAAC 
AGCAACATAA GTCAAACCAG CAAGGCATCA AGGACCCTGC CGGTGCTGCC AGTGAATGGG 
AAGATGCACA GCACGGTGGA TTGCAATGGA GTGGTCTCTC TACTTGGTGG CCCATCAGGT 
CCAACATCGC CTGCTGGACT ACTTCTGCCA GAGGTGATAA TAGATAAACC AGCTTCTGAT 
GACAATGGAA CTAATACAGA GAATGAACTG AGGAAAAGAC GGCTCAGTTC GTATCATGTT 
TCTATGGATT TTCTGGAAGA TCCGATTCTC AGACAAAGGG CTCTGAGCAT TGCCAGCAAT 
CTAACAAATA CAATGGAAGA ACTTGAAGAA TCCAGGGAAA AATGTCCACC ATGCTGGAAT 
AAATTTGCCA ATACATATCT AATCTGGGAT TGCAGCGACT CTTGGCTAAA GATAAAGCAT 
GTTGTCAACT TGATTGTCAT GGACCCATTT GTGGATCTTG CAATAACAAT CTGCATTGTT 
TTGAACACGT TATTTATGGC TATGGAGCAT TACCCAATAA CCGATGAATT TGCCGGTGCG 
CTCAATGTGG GAAATTTGGT GTTTACAGGT ATTTTTACTG CTGAGATGGT TTTCAAGCTA 
ATTGCCCTAG ATCCCTATTA CTATTTCCAA GTTGGCTGGA ACATATTCGA TGGGGTTATT 
GTCAGTCTCA GCTTGATGGA ACTTGGTCTT TCGGATGTGG AAGGGTTATC AGTCCTTCGA 
TCTTTCAGAT TGCTTCGTGT ATTCAAATTG GCGAAATCCT GGCCTACACT GAACATGCTG 
ATAAAAATTA TTGGCAATTC TGTAGGAGCT TTGGGGAACC TGACTTTGGT GTTGGCCATC 
ATTGTCTTCA TTTTTGCTGT GGTTGGGATG CAGCTGTTTG GTAAAAGTTA CAAGGAATGT 
GTCTGCAAAA TTGCCCCAGA TGACTGTGAG CTTCCTCGCT GGCATATGAA CGACTTCTTC 
CACTCCTTCC TTATTGTGTT CCGAGTTCTG TGTGGGGAAT GGATTGAAAC CATGTGGGAC 
TGTATGGAGG TTGCAGGACA AGCTATGTGT CTTACTGTAT TTATGATGGT GATGGTTATT 
GGAAACCTAG TGGTGTTAAA TTTGTTTTTA GCTTTGCTGC TCAGCTCATT TAGCTCAGAC 
AATCTAGCTG CTACAGAGGA CGATAATGAA ATGAACAATC TACAAATTGC AGTGGCCAGA 
ATGCACGAAG GAATAGCCTA TGTGAAAAGG AAGATGCGGG AATTCATACA AAACGCCTTT 
GTCAAAAAGC AAAAAGCCTT AGAAGAAACC AAACCCTTAG AAGACATGCA GAACCAAAAG 
GACAGCTGCA TTTCCAATCA CACCACTGTG GAAATAAGCA AAGAGTATGA CTACCTGAAA 
GATGGAAATG TGACAACGGC CACCAGTGGC ATAGGCAGCA GTGTGGAAAA GTACATCATA 
GACGAAAGCG ACTACATGTC TTTCATTCAT AACCCAAGCC TAACTGTCAC TGTACCTATT 
GCTGTGGGGG AGTCCGATTT TGAGAATCTG AACACAGAGG AGTTTAGCAG CGAATCAGAC 
TTGGAAGAAA GCAAGGAGAA GCTCAACTCA TCCAGCTCAT CAGAAGGCAG TACGGTGGAC 
ATCAGGCCTG CTGGGGAGGG AGAGCCTGTT CCACAGGTGG AGGTTGAAGA GTTCCAGGAA 
CCGGATGCTT GTTTCACAGA TGGCTGTATC AGGAGATTTC CATGCTGTGT GGTTGATGTC 
TCACATGGCA AATGGAAGCA GTGGTGGAAC CTCAGAAAAA CATGTTTCTT AATCGTAGAA 
CACAACTGGT TTGAGACCTT CATAATCTTT ATGATTCTTA TGAGTAGTGG TGCCCTGGCA 
TTTGAAGATA TCTACATAGA ACAAAGAAAA ACAATAAAAA TAATTTTGGA ATATGCGGAC 
AAGGTTTTTA CGTACATCTT TATCTTGGAA ATGCTTCTGA AATGGGTGGC ATATGGCTTT 
CAAAAATATT TCACAAATGC CTGGTGCTGG TTGGACTTCT TGATTGTTGA TATTTCAATC 
ATATCCCTTT TGGCGGTGGC TCTTGGGTAT GATCAACTAG CCGCCATTAA ATCTCTAAGG 
ACCTTGAGAG CACTGAGACC TCTGCGTGCA TTATCAAGAT TTGAAGGAAT AAGGGTTGTA 
GTGAATGCCC TCGTTGGAGC TATTCCATCC ATTATGAACG TTCTTCTGGT TTGTTTAATA 
TTTTGGCTCA TTTTTAGCAT TATGGGAGTA AACATGTTTT CCGGAAAGTA TGGCTATTGT 
GTGAATTACA CAGATGACGT AGTGTTTGAC TACACAATAG TTAACAATAG GAGCCAATGT 
ATGGACCTAA TTAGTGAAAA TAAAACGGCC AGATGGAAAA ACGTAAAAGT GAATTTTGAT 
AACGTGGGAT TTGGATATCT TGCTTTGCTT CAAGTGGCAA CATTCAAAGG ATGGATGGAA 
ATAATGTATG CAGCAGTTGA CTCAAGAAAG CAAGAGGAAC AGCCAAAATA TGAAGACAAT 
CTGTACATGT ATTTGTACTT CGTTATTTTC ATCATTTTTG GATCGTTTTT CACACTCAAC 
CTTTTCATTG GTGTCATCAT TGATAATTTT AATCAGCAGA AGAAGAAGTT CGGAGGTCAA 
GACATCTTTA TGACGGAAGA ACAAAAAAAA TATTACAATG CAATGAAGAA GCTGGGGTCC 
AAGAAACCAC AAAAGCCTGT TCCAAGACCT GATAATAAAT TTCAAGGTGC CGTCTTTGAT 
TTTGTGTCGA AACAACCGTT TGATATAGCC ATCATGATTC TGATCTGTTT AAATATGGTA 
ACCATGATGA TTGAAACAGA TGATCAGAGC ATCGAAACGG AGAACAACTT GTACTGGATA 
AATGCAGTTT TCATTGTTCT TTTTACTGGG GAGTGTGTGC TCAAGCTGAT ATCCCTACGT 
CATTATTACT TCACAATTGG CTGGAACATA TTTGATTTTG TGGTTGTAAT TCTCTCTATA 
GTAGGTATGT TTCTGGCAGA CCTTATCGAA AAATATTTTG TGTCACCCAC CCTGTTCAGG 
GTCATCCGAT TAGCTAGGAT TGGTCGTATC CTGCGTCTCA TCAAGGGAGC TAAAGGCATT 
CGTACTCTGC TGTTTGCCCT GATGATGTCA CTTCCTGCGT TGTTCAATAT TGGTTTACTG 
CTCTTTTTGG TGATGTTCAT CTACGCCATT TTCGGCATGT CCAACTTTGC CTATGTTAAG 
AAAGAGGCTG GGATTGATGA CATGTTTAAC TTTGAGACCT TTGGTAACAG CATGATCTGT 
TTATTCCAAA TTACAACATC TGCAGGATGG GATGGTCTGT TAGCGCCAAT TCTTAATAGC 
GGGGCACCAG ATTGCGATCC AAAGGCAGAA CATTCAGGAA GCAGTGTAAA AGGAGACTGT 
GGCAATCCCT CTGTTGGCAT CTTCTTTTTT GTTAGTTATA TCATTATATC ATTCTTGGTT 
GTAGTCAACA TGTATATTGC TGTCATCTTG GAAAACTTCA GTGTTGCTAC GGAGGAAAGT 
GCCGAACCTC TAGGAGAAGA TGATTTTGAG ATGTTCTATG AGGTCTGGGA AAAATTTGAT 
TCAAATGCAA CACAGTTTAT ACAATACGTT AAACTGACTG ATTTTGCTGA TGCGCTGGAT 
CCGCCTCTCC GAATGCCGAA ACCAAACAAT GCGCAGTTAA TCGCAATGGA TCTGCCTATG 
GTAAGTGGGG ACAGAATTCA CTGCCTTGAT GTCCTGTTTG CCTTTACAAA GCGTGTGCTA 
GGTGAAGGCG ATGAAATGGA TAACTTAAGA CAGCCAATGG AAGAACGTTT CATGGCATCC 
AATCCTTCAA AGGCCTCCTA CGAACCAATC ACGTCTACTC TACGTAGGAA ACAAGAGGAG 
TTAGCTGCCA TAGTTATCCA GCGCTGCTAC AGATGCTACG CTTTAAGGAA AGGCATAAAA 
CATGCCTCCT TTATGTACAA CCAGGAAAAG GGCAAAGATG GAGGGAGTCT ACTTACTAGG 
AAAGATATGC TTAGCGATCG ACTAAATGGA AACTCTACAA CAGAGAAAGC AGACATTACC 
CCTTCGACAA CGTCTCCTCC GTCCTATGAC AGTGTTACAA AACCAGATAA AGAAAAACAC 
GAAAAAGACA AAATAGAGAA GGAAGACAGG GGCAAAGATG TCAGGGAGCA TAAAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

scn3a ( XM_031892885.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031748745.1 Xenopus tropicalis sodium channel, voltage gated, type III alpha subunit (scn3a), transcript variant X4, mRNA.

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CloneID	OXa66282
Clone ID Related Accession (Same CDS sequence)	XM_031892885.1
Accession Version	XM_031892885.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6027bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sodium channel protein type 2 subunit alpha isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGC CACTGCTACC CCCACCAGGA CCTGACAGCC TTCGACTCCT CACCAGAGAA 
TCTCTTGCTG TTCTTGAAAA GCGCTTTGCA GAAGAAAAAG CCAGGAAGGC ATCACAGAAG 
GATCGCAGAG ATGATGATGA TGAAAATGGA CCCAAGCCTA ATTCGGACTT GGAAGCTGGC 
AAAAACTTAC CATTTATATA TGGAGACATT CCACAGGGAA TGGTGGCAGT ACCAATGGAA 
GACCTGGACC CATATTACAG CAACCAAAAG ACCTTTATAG TATTGAATAG AGGGAAGGCA 
ATCTTCCGAT TCAGTGCCAC ATCTGCCTTG TACATTTTAA CTCCTTTCAA TCCTCTTAGA 
AGAATATCTA TAAAGATTTT GGTACATTCT TTATTCAGCA TGCTCATTAT GTGCACTATT 
TTGACTAATT GCTTCTTCAT GACCATGAGT AACCCACCAG AATGGACCAA GAATGTTGAA 
TATACCTTCA CTGGAATTTA TACATTTGAA TCACTTGTTA AAATACTTGC CAGAGGTTTT 
TGTTTAGAAA GCTTTACTTT TCTACGGGAT CCATGGAATT GGCTTGATTT CAGCGTCATA 
GTCATGGCAT ATGTGACAGA GTTTGTGGAC CTGGGCAATG TCTCAGCATT GAGAACATTC 
AGAGTTCTCC GAGCATTGAA AACAATATCA GTCATTCCAG GTCTGAAGAC TATCGTGGGC 
GCACTGATTC AGTCGGTGAA GAAGCTTTCC GATGTAATGA TTCTCACAGT GTTTTGCCTC 
AGTGTGTTTG CACTAATAGG GCTACAGCTT TTCATGGGGC ACCTGAGGAA TAAGTGTGTA 
AGGTGGCCAC CTGAGTATGA ACCAAATATC ACTTCCTACT TTAATGAAAC TGCTGGCCTC 
TATATTAATG TAACTCTGAA CCCATTCAAT CGTGAAGAAT ACATCCTAGA AGAAACTAAT 
TTTTATTTTT TGCCTGGAAC GAGAGATGCT CTTCTGTGTG GCAACAGCTC CGATGCAGGG 
CAGTGTCCAA AAGGATATGT ATGTTTGAAG ACAGGAAGGA ACCCAAACTA CGGCTACACA 
AGTTTTGACA CATTTAGCTG GGCTTTTTTA TCACTGTTTC GGCTAATGAC TCAGGATTTC 
TGGGAAAATC TTTATCAACT GACACTACGA GCTGCTGGTA AAACCTACAT GATATTTTTC 
GTTTTGGTGA TTTTCCTGGG TTCTTTCTAT TTGATAAACT TGATCTTGGC TGTGGTGGCC 
ATGGCTTATG AAGAGCAGAA TCAGGCAACC TTAGAAGAAG CGGAGCAGAA AGAAGCAGAA 
TTCCAGCTTA TGATGGAGCA ACTGAAAAAA CATCAGGAAG AAATTCAGGC AGCGGCGCTA 
GCAGCAGAAA CAATGGACGG TTCCAGAGAG TTTAGTGGTG GGACAGGCTA TGGTGGGCTT 
TCAGAAAGCT CTTCTGAGGC ATCAAAGCCA AGTTCCAAAA GTGCTAAGGA GAGAAGAAAT 
AGAAGGAAAA AGAGAAAACA AAAAGAGCAG GGTGAGGATG AGAAGGCAGA TGATGAAAAA 
TTCCACAAGT CGGCTTCAGA AACGAGTATA AAGAGGAAAG GCTTTCGTTT TTCTATAGAA 
GGAAACCGAC TGACCTATGA GAAGAAGTAC TCGTCCCCAC ATCAGTCCCG CCTCAGTTTC 
CGTGGCTCCC TCTTTTTCCC AAGGCGTGAT AGCAGAGCGA GCCTGTGCAG CTTCAGAGGC 
CGTGGGAAGG ATGTAGGATC AGAAAATGAC TTTGCGGATG ATGAACACAG CACATTTGAG 
GACAATGAAA GCAGAAGAGG TTCATTGTTC GTTCCACACA GGCATGGAGA GAGGAGGAAC 
AGCAACATAA GTCAAACCAG CAAGGCATCA AGGACCCTGC CGGTGCTGCC AGTGAATGGG 
AAGATGCACA GCACGGTGGA TTGCAATGGA GTGGTCTCTC TACTTGGTGG CCCATCAGGT 
CCAACATCGC CTGCTGGACT ACTTCTGCCA GAGGGAACTA ATACAGAGAA TGAACTGAGG 
AAAAGACGGC TCAGTTCGTA TCATGTTTCT ATGGATTTTC TGGAAGATCC GATTCTCAGA 
CAAAGGGCTC TGAGCATTGC CAGCAATCTA ACAAATACAA TGGAAGAACT TGAAGAATCC 
AGGGAAAAAT GTCCACCATG CTGGAATAAA TTTGCCAATA CATATCTAAT CTGGGATTGC 
AGCGACTCTT GGCTAAAGAT AAAGCATGTT GTCAACTTGA TTGTCATGGA CCCATTTGTG 
GATCTTGCAA TAACAATCTG CATTGTTTTG AACACGTTAT TTATGGCTAT GGAGCATTAC 
CCAATAACCG ATGAATTTGC CGGTGCGCTC AATGTGGGAA ATTTGGTGTT TACAGGTATT 
TTTACTGCTG AGATGGTTTT CAAGCTAATT GCCCTAGATC CCTATTACTA TTTCCAAGTT 
GGCTGGAACA TATTCGATGG GGTTATTGTC AGTCTCAGCT TGATGGAACT TGGTCTTTCG 
GATGTGGAAG GGTTATCAGT CCTTCGATCT TTCAGATTGC TTCGTGTATT CAAATTGGCG 
AAATCCTGGC CTACACTGAA CATGCTGATA AAAATTATTG GCAATTCTGT AGGAGCTTTG 
GGGAACCTGA CTTTGGTGTT GGCCATCATT GTCTTCATTT TTGCTGTGGT TGGGATGCAG 
CTGTTTGGTA AAAGTTACAA GGAATGTGTC TGCAAAATTG CCCCAGATGA CTGTGAGCTT 
CCTCGCTGGC ATATGAACGA CTTCTTCCAC TCCTTCCTTA TTGTGTTCCG AGTTCTGTGT 
GGGGAATGGA TTGAAACCAT GTGGGACTGT ATGGAGGTTG CAGGACAAGC TATGTGTCTT 
ACTGTATTTA TGATGGTGAT GGTTATTGGA AACCTAGTGG TGTTAAATTT GTTTTTAGCT 
TTGCTGCTCA GCTCATTTAG CTCAGACAAT CTAGCTGCTA CAGAGGACGA TAATGAAATG 
AACAATCTAC AAATTGCAGT GGCCAGAATG CACGAAGGAA TAGCCTATGT GAAAAGGAAG 
ATGCGGGAAT TCATACAAAA CGCCTTTGTC AAAAAGCAAA AAGCCTTAGA AGAAACCAAA 
CCCTTAGAAG ACATGCAGAA CCAAAAGGAC AGCTGCATTT CCAATCACAC CACTGTGGAA 
ATAAGCAAAG AGTATGACTA CCTGAAAGAT GGAAATGTGA CAACGGCCAC CAGTGGCATA 
GGCAGCAGTG TGGAAAAGTA CATCATAGAC GAAAGCGACT ACATGTCTTT CATTCATAAC 
CCAAGCCTAA CTGTCACTGT ACCTATTGCT GTGGGGGAGT CCGATTTTGA GAATCTGAAC 
ACAGAGGAGT TTAGCAGCGA ATCAGACTTG GAAGAAAGCA AGGAGAAGCT CAACTCATCC 
AGCTCATCAG AAGGCAGTAC GGTGGACATC AGGCCTGCTG GGGAGGGAGA GCCTGTTCCA 
CAGGTGGAGG TTGAAGAGTT CCAGGAACCG GATGCTTGTT TCACAGATGG CTGTATCAGG 
AGATTTCCAT GCTGTGTGGT TGATGTCTCA CATGGCAAAT GGAAGCAGTG GTGGAACCTC 
AGAAAAACAT GTTTCTTAAT CGTAGAACAC AACTGGTTTG AGACCTTCAT AATCTTTATG 
ATTCTTATGA GTAGTGGTGC CCTGGCATTT GAAGATATCT ACATAGAACA AAGAAAAACA 
ATAAAAATAA TTTTGGAATA TGCGGACAAG GTTTTTACGT ACATCTTTAT CTTGGAAATG 
CTTCTGAAAT GGGTGGCATA TGGCTTTCAA AAATATTTCA CAAATGCCTG GTGCTGGTTG 
GACTTCTTGA TTGTTGATGT TTCTTTGGTT AGCTTAATAG CTAATGCCTT GGGCTACTCA 
GAATTAGGAG CTATTAAGTC CCTTAGGACA CTAAGGGCAT TGAGACCTCT GAGGGCCCTC 
TCACGGTTTG AAGGGATGAG GGTTGTAGTG AATGCCCTCG TTGGAGCTAT TCCATCCATT 
ATGAACGTTC TTCTGGTTTG TTTAATATTT TGGCTCATTT TTAGCATTAT GGGAGTAAAC 
ATGTTTTCCG GAAAGTATGG CTATTGTGTG AATTACACAG ATGACGTAGT GTTTGACTAC 
ACAATAGTTA ACAATAGGAG CCAATGTATG GACCTAATTA GTGAAAATAA AACGGCCAGA 
TGGAAAAACG TAAAAGTGAA TTTTGATAAC GTGGGATTTG GATATCTTGC TTTGCTTCAA 
GTGGCAACAT TCAAAGGATG GATGGAAATA ATGTATGCAG CAGTTGACTC AAGAAAGCAA 
GAGGAACAGC CAAAATATGA AGACAATCTG TACATGTATT TGTACTTCGT TATTTTCATC 
ATTTTTGGAT CGTTTTTCAC ACTCAACCTT TTCATTGGTG TCATCATTGA TAATTTTAAT 
CAGCAGAAGA AGAAGTTCGG AGGTCAAGAC ATCTTTATGA CGGAAGAACA AAAAAAATAT 
TACAATGCAA TGAAGAAGCT GGGGTCCAAG AAACCACAAA AGCCTGTTCC AAGACCTGAT 
AATAAATTTC AAGGTGCCGT CTTTGATTTT GTGTCGAAAC AACCGTTTGA TATAGCCATC 
ATGATTCTGA TCTGTTTAAA TATGGTAACC ATGATGATTG AAACAGATGA TCAGAGCATC 
GAAACGGAGA ACAACTTGTA CTGGATAAAT GCAGTTTTCA TTGTTCTTTT TACTGGGGAG 
TGTGTGCTCA AGCTGATATC CCTACGTCAT TATTACTTCA CAATTGGCTG GAACATATTT 
GATTTTGTGG TTGTAATTCT CTCTATAGTA GGTATGTTTC TGGCAGACCT TATCGAAAAA 
TATTTTGTGT CACCCACCCT GTTCAGGGTC ATCCGATTAG CTAGGATTGG TCGTATCCTG 
CGTCTCATCA AGGGAGCTAA AGGCATTCGT ACTCTGCTGT TTGCCCTGAT GATGTCACTT 
CCTGCGTTGT TCAATATTGG TTTACTGCTC TTTTTGGTGA TGTTCATCTA CGCCATTTTC 
GGCATGTCCA ACTTTGCCTA TGTTAAGAAA GAGGCTGGGA TTGATGACAT GTTTAACTTT 
GAGACCTTTG GTAACAGCAT GATCTGTTTA TTCCAAATTA CAACATCTGC AGGATGGGAT 
GGTCTGTTAG CGCCAATTCT TAATAGCGGG GCACCAGATT GCGATCCAAA GGCAGAACAT 
TCAGGAAGCA GTGTAAAAGG AGACTGTGGC AATCCCTCTG TTGGCATCTT CTTTTTTGTT 
AGTTATATCA TTATATCATT CTTGGTTGTA GTCAACATGT ATATTGCTGT CATCTTGGAA 
AACTTCAGTG TTGCTACGGA GGAAAGTGCC GAACCTCTAG GAGAAGATGA TTTTGAGATG 
TTCTATGAGG TCTGGGAAAA ATTTGATTCA AATGCAACAC AGTTTATACA ATACGTTAAA 
CTGACTGATT TTGCTGATGC GCTGGATCCG CCTCTCCGAA TGCCGAAACC AAACAATGCG 
CAGTTAATCG CAATGGATCT GCCTATGGTA AGTGGGGACA GAATTCACTG CCTTGATGTC 
CTGTTTGCCT TTACAAAGCG TGTGCTAGGT GAAGGCGATG AAATGGATAA CTTAAGACAG 
CCAATGGAAG AACGTTTCAT GGCATCCAAT CCTTCAAAGG CCTCCTACGA ACCAATCACG 
TCTACTCTAC GTAGGAAACA AGAGGAGTTA GCTGCCATAG TTATCCAGCG CTGCTACAGA 
TGCTACGCTT TAAGGAAAGG CATAAAACAT GCCTCCTTTA TGTACAACCA GGAAAAGGGC 
AAAGATGGAG GGAGTCTACT TACTAGGAAA GATATGCTTA GCGATCGACT AAATGGAAAC 
TCTACAACAG AGAAAGCAGA CATTACCCCT TCGACAACGT CTCCTCCGTC CTATGACAGT 
GTTACAAAAC CAGATAAAGA AAAACACGAA AAAGACAAAA TAGAGAAGGA AGACAGGGGC 
AAAGATGTCA GGGAGCATAA AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031748745.1
CDS	285..6311
Translation	MEQPLLPPPGPDSLRLLTRESLAVLEKRFAEEKARKASQKDRRDDDDENGPKPNSDLEAGKNLPFIYGDIPQGMVAVPMEDLDPYYSNQKTFIVLNRGKAIFRFSATSALYILTPFNPLRRISIKILVHSLFSMLIMCTILTNCFFMTMSNPPEWTKNVEYTFTGIYTFESLVKILARGFCLESFTFLRDPWNWLDFSVIVMAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGHLRNKCVRWPPEYEPNITSYFNETAGLYINVTLNPFNREEYILEETNFYFLPGTRDALLCGNSSDAGQCPKGYVCLKTGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQLMMEQLKKHQEEIQAAALAAETMDGSREFSGGTGYGGLSESSSEASKPSSKSAKERRNRRKKRKQKEQGEDEKADDEKFHKSASETSIKRKGFRFSIEGNRLTYEKKYSSPHQSRLSFRGSLFFPRRDSRASLCSFRGRGKDVGSENDFADDEHSTFEDNESRRGSLFVPHRHGERRNSNISQTSKASRTLPVLPVNGKMHSTVDCNGVVSLLGGPSGPTSPAGLLLPEGTNTENELRKRRLSSYHVSMDFLEDPILRQRALSIASNLTNTMEELEESREKCPPCWNKFANTYLIWDCSDSWLKIKHVVNLIVMDPFVDLAITICIVLNTLFMAMEHYPITDEFAGALNVGNLVFTGIFTAEMVFKLIALDPYYYFQVGWNIFDGVIVSLSLMELGLSDVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKIAPDDCELPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSSDNLAATEDDNEMNNLQIAVARMHEGIAYVKRKMREFIQNAFVKKQKALEETKPLEDMQNQKDSCISNHTTVEISKEYDYLKDGNVTTATSGIGSSVEKYIIDESDYMSFIHNPSLTVTVPIAVGESDFENLNTEEFSSESDLEESKEKLNSSSSSEGSTVDIRPAGEGEPVPQVEVEEFQEPDACFTDGCIRRFPCCVVDVSHGKWKQWWNLRKTCFLIVEHNWFETFIIFMILMSSGALAFEDIYIEQRKTIKIILEYADKVFTYIFILEMLLKWVAYGFQKYFTNAWCWLDFLIVDVSLVSLIANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNMFSGKYGYCVNYTDDVVFDYTIVNNRSQCMDLISENKTARWKNVKVNFDNVGFGYLALLQVATFKGWMEIMYAAVDSRKQEEQPKYEDNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPVPRPDNKFQGAVFDFVSKQPFDIAIMILICLNMVTMMIETDDQSIETENNLYWINAVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLADLIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKKEAGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSGAPDCDPKAEHSGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLGEDDFEMFYEVWEKFDSNATQFIQYVKLTDFADALDPPLRMPKPNNAQLIAMDLPMVSGDRIHCLDVLFAFTKRVLGEGDEMDNLRQPMEERFMASNPSKASYEPITSTLRRKQEELAAIVIQRCYRCYALRKGIKHASFMYNQEKGKDGGSLLTRKDMLSDRLNGNSTTEKADITPSTTSPPSYDSVTKPDKEKHEKDKIEKEDRGKDVREHKK

Target ORF information:

RefSeq Version	XM_031892885.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sodium channel, voltage gated, type III alpha subunit (scn3a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031892885.1

ORF Insert Sequence:

ATGGAGCAGC CACTGCTACC CCCACCAGGA CCTGACAGCC TTCGACTCCT CACCAGAGAA 
TCTCTTGCTG TTCTTGAAAA GCGCTTTGCA GAAGAAAAAG CCAGGAAGGC ATCACAGAAG 
GATCGCAGAG ATGATGATGA TGAAAATGGA CCCAAGCCTA ATTCGGACTT GGAAGCTGGC 
AAAAACTTAC CATTTATATA TGGAGACATT CCACAGGGAA TGGTGGCAGT ACCAATGGAA 
GACCTGGACC CATATTACAG CAACCAAAAG ACCTTTATAG TATTGAATAG AGGGAAGGCA 
ATCTTCCGAT TCAGTGCCAC ATCTGCCTTG TACATTTTAA CTCCTTTCAA TCCTCTTAGA 
AGAATATCTA TAAAGATTTT GGTACATTCT TTATTCAGCA TGCTCATTAT GTGCACTATT 
TTGACTAATT GCTTCTTCAT GACCATGAGT AACCCACCAG AATGGACCAA GAATGTTGAA 
TATACCTTCA CTGGAATTTA TACATTTGAA TCACTTGTTA AAATACTTGC CAGAGGTTTT 
TGTTTAGAAA GCTTTACTTT TCTACGGGAT CCATGGAATT GGCTTGATTT CAGCGTCATA 
GTCATGGCAT ATGTGACAGA GTTTGTGGAC CTGGGCAATG TCTCAGCATT GAGAACATTC 
AGAGTTCTCC GAGCATTGAA AACAATATCA GTCATTCCAG GTCTGAAGAC TATCGTGGGC 
GCACTGATTC AGTCGGTGAA GAAGCTTTCC GATGTAATGA TTCTCACAGT GTTTTGCCTC 
AGTGTGTTTG CACTAATAGG GCTACAGCTT TTCATGGGGC ACCTGAGGAA TAAGTGTGTA 
AGGTGGCCAC CTGAGTATGA ACCAAATATC ACTTCCTACT TTAATGAAAC TGCTGGCCTC 
TATATTAATG TAACTCTGAA CCCATTCAAT CGTGAAGAAT ACATCCTAGA AGAAACTAAT 
TTTTATTTTT TGCCTGGAAC GAGAGATGCT CTTCTGTGTG GCAACAGCTC CGATGCAGGG 
CAGTGTCCAA AAGGATATGT ATGTTTGAAG ACAGGAAGGA ACCCAAACTA CGGCTACACA 
AGTTTTGACA CATTTAGCTG GGCTTTTTTA TCACTGTTTC GGCTAATGAC TCAGGATTTC 
TGGGAAAATC TTTATCAACT GACACTACGA GCTGCTGGTA AAACCTACAT GATATTTTTC 
GTTTTGGTGA TTTTCCTGGG TTCTTTCTAT TTGATAAACT TGATCTTGGC TGTGGTGGCC 
ATGGCTTATG AAGAGCAGAA TCAGGCAACC TTAGAAGAAG CGGAGCAGAA AGAAGCAGAA 
TTCCAGCTTA TGATGGAGCA ACTGAAAAAA CATCAGGAAG AAATTCAGGC AGCGGCGCTA 
GCAGCAGAAA CAATGGACGG TTCCAGAGAG TTTAGTGGTG GGACAGGCTA TGGTGGGCTT 
TCAGAAAGCT CTTCTGAGGC ATCAAAGCCA AGTTCCAAAA GTGCTAAGGA GAGAAGAAAT 
AGAAGGAAAA AGAGAAAACA AAAAGAGCAG GGTGAGGATG AGAAGGCAGA TGATGAAAAA 
TTCCACAAGT CGGCTTCAGA AACGAGTATA AAGAGGAAAG GCTTTCGTTT TTCTATAGAA 
GGAAACCGAC TGACCTATGA GAAGAAGTAC TCGTCCCCAC ATCAGTCCCG CCTCAGTTTC 
CGTGGCTCCC TCTTTTTCCC AAGGCGTGAT AGCAGAGCGA GCCTGTGCAG CTTCAGAGGC 
CGTGGGAAGG ATGTAGGATC AGAAAATGAC TTTGCGGATG ATGAACACAG CACATTTGAG 
GACAATGAAA GCAGAAGAGG TTCATTGTTC GTTCCACACA GGCATGGAGA GAGGAGGAAC 
AGCAACATAA GTCAAACCAG CAAGGCATCA AGGACCCTGC CGGTGCTGCC AGTGAATGGG 
AAGATGCACA GCACGGTGGA TTGCAATGGA GTGGTCTCTC TACTTGGTGG CCCATCAGGT 
CCAACATCGC CTGCTGGACT ACTTCTGCCA GAGGGAACTA ATACAGAGAA TGAACTGAGG 
AAAAGACGGC TCAGTTCGTA TCATGTTTCT ATGGATTTTC TGGAAGATCC GATTCTCAGA 
CAAAGGGCTC TGAGCATTGC CAGCAATCTA ACAAATACAA TGGAAGAACT TGAAGAATCC 
AGGGAAAAAT GTCCACCATG CTGGAATAAA TTTGCCAATA CATATCTAAT CTGGGATTGC 
AGCGACTCTT GGCTAAAGAT AAAGCATGTT GTCAACTTGA TTGTCATGGA CCCATTTGTG 
GATCTTGCAA TAACAATCTG CATTGTTTTG AACACGTTAT TTATGGCTAT GGAGCATTAC 
CCAATAACCG ATGAATTTGC CGGTGCGCTC AATGTGGGAA ATTTGGTGTT TACAGGTATT 
TTTACTGCTG AGATGGTTTT CAAGCTAATT GCCCTAGATC CCTATTACTA TTTCCAAGTT 
GGCTGGAACA TATTCGATGG GGTTATTGTC AGTCTCAGCT TGATGGAACT TGGTCTTTCG 
GATGTGGAAG GGTTATCAGT CCTTCGATCT TTCAGATTGC TTCGTGTATT CAAATTGGCG 
AAATCCTGGC CTACACTGAA CATGCTGATA AAAATTATTG GCAATTCTGT AGGAGCTTTG 
GGGAACCTGA CTTTGGTGTT GGCCATCATT GTCTTCATTT TTGCTGTGGT TGGGATGCAG 
CTGTTTGGTA AAAGTTACAA GGAATGTGTC TGCAAAATTG CCCCAGATGA CTGTGAGCTT 
CCTCGCTGGC ATATGAACGA CTTCTTCCAC TCCTTCCTTA TTGTGTTCCG AGTTCTGTGT 
GGGGAATGGA TTGAAACCAT GTGGGACTGT ATGGAGGTTG CAGGACAAGC TATGTGTCTT 
ACTGTATTTA TGATGGTGAT GGTTATTGGA AACCTAGTGG TGTTAAATTT GTTTTTAGCT 
TTGCTGCTCA GCTCATTTAG CTCAGACAAT CTAGCTGCTA CAGAGGACGA TAATGAAATG 
AACAATCTAC AAATTGCAGT GGCCAGAATG CACGAAGGAA TAGCCTATGT GAAAAGGAAG 
ATGCGGGAAT TCATACAAAA CGCCTTTGTC AAAAAGCAAA AAGCCTTAGA AGAAACCAAA 
CCCTTAGAAG ACATGCAGAA CCAAAAGGAC AGCTGCATTT CCAATCACAC CACTGTGGAA 
ATAAGCAAAG AGTATGACTA CCTGAAAGAT GGAAATGTGA CAACGGCCAC CAGTGGCATA 
GGCAGCAGTG TGGAAAAGTA CATCATAGAC GAAAGCGACT ACATGTCTTT CATTCATAAC 
CCAAGCCTAA CTGTCACTGT ACCTATTGCT GTGGGGGAGT CCGATTTTGA GAATCTGAAC 
ACAGAGGAGT TTAGCAGCGA ATCAGACTTG GAAGAAAGCA AGGAGAAGCT CAACTCATCC 
AGCTCATCAG AAGGCAGTAC GGTGGACATC AGGCCTGCTG GGGAGGGAGA GCCTGTTCCA 
CAGGTGGAGG TTGAAGAGTT CCAGGAACCG GATGCTTGTT TCACAGATGG CTGTATCAGG 
AGATTTCCAT GCTGTGTGGT TGATGTCTCA CATGGCAAAT GGAAGCAGTG GTGGAACCTC 
AGAAAAACAT GTTTCTTAAT CGTAGAACAC AACTGGTTTG AGACCTTCAT AATCTTTATG 
ATTCTTATGA GTAGTGGTGC CCTGGCATTT GAAGATATCT ACATAGAACA AAGAAAAACA 
ATAAAAATAA TTTTGGAATA TGCGGACAAG GTTTTTACGT ACATCTTTAT CTTGGAAATG 
CTTCTGAAAT GGGTGGCATA TGGCTTTCAA AAATATTTCA CAAATGCCTG GTGCTGGTTG 
GACTTCTTGA TTGTTGATGT TTCTTTGGTT AGCTTAATAG CTAATGCCTT GGGCTACTCA 
GAATTAGGAG CTATTAAGTC CCTTAGGACA CTAAGGGCAT TGAGACCTCT GAGGGCCCTC 
TCACGGTTTG AAGGGATGAG GGTTGTAGTG AATGCCCTCG TTGGAGCTAT TCCATCCATT 
ATGAACGTTC TTCTGGTTTG TTTAATATTT TGGCTCATTT TTAGCATTAT GGGAGTAAAC 
ATGTTTTCCG GAAAGTATGG CTATTGTGTG AATTACACAG ATGACGTAGT GTTTGACTAC 
ACAATAGTTA ACAATAGGAG CCAATGTATG GACCTAATTA GTGAAAATAA AACGGCCAGA 
TGGAAAAACG TAAAAGTGAA TTTTGATAAC GTGGGATTTG GATATCTTGC TTTGCTTCAA 
GTGGCAACAT TCAAAGGATG GATGGAAATA ATGTATGCAG CAGTTGACTC AAGAAAGCAA 
GAGGAACAGC CAAAATATGA AGACAATCTG TACATGTATT TGTACTTCGT TATTTTCATC 
ATTTTTGGAT CGTTTTTCAC ACTCAACCTT TTCATTGGTG TCATCATTGA TAATTTTAAT 
CAGCAGAAGA AGAAGTTCGG AGGTCAAGAC ATCTTTATGA CGGAAGAACA AAAAAAATAT 
TACAATGCAA TGAAGAAGCT GGGGTCCAAG AAACCACAAA AGCCTGTTCC AAGACCTGAT 
AATAAATTTC AAGGTGCCGT CTTTGATTTT GTGTCGAAAC AACCGTTTGA TATAGCCATC 
ATGATTCTGA TCTGTTTAAA TATGGTAACC ATGATGATTG AAACAGATGA TCAGAGCATC 
GAAACGGAGA ACAACTTGTA CTGGATAAAT GCAGTTTTCA TTGTTCTTTT TACTGGGGAG 
TGTGTGCTCA AGCTGATATC CCTACGTCAT TATTACTTCA CAATTGGCTG GAACATATTT 
GATTTTGTGG TTGTAATTCT CTCTATAGTA GGTATGTTTC TGGCAGACCT TATCGAAAAA 
TATTTTGTGT CACCCACCCT GTTCAGGGTC ATCCGATTAG CTAGGATTGG TCGTATCCTG 
CGTCTCATCA AGGGAGCTAA AGGCATTCGT ACTCTGCTGT TTGCCCTGAT GATGTCACTT 
CCTGCGTTGT TCAATATTGG TTTACTGCTC TTTTTGGTGA TGTTCATCTA CGCCATTTTC 
GGCATGTCCA ACTTTGCCTA TGTTAAGAAA GAGGCTGGGA TTGATGACAT GTTTAACTTT 
GAGACCTTTG GTAACAGCAT GATCTGTTTA TTCCAAATTA CAACATCTGC AGGATGGGAT 
GGTCTGTTAG CGCCAATTCT TAATAGCGGG GCACCAGATT GCGATCCAAA GGCAGAACAT 
TCAGGAAGCA GTGTAAAAGG AGACTGTGGC AATCCCTCTG TTGGCATCTT CTTTTTTGTT 
AGTTATATCA TTATATCATT CTTGGTTGTA GTCAACATGT ATATTGCTGT CATCTTGGAA 
AACTTCAGTG TTGCTACGGA GGAAAGTGCC GAACCTCTAG GAGAAGATGA TTTTGAGATG 
TTCTATGAGG TCTGGGAAAA ATTTGATTCA AATGCAACAC AGTTTATACA ATACGTTAAA 
CTGACTGATT TTGCTGATGC GCTGGATCCG CCTCTCCGAA TGCCGAAACC AAACAATGCG 
CAGTTAATCG CAATGGATCT GCCTATGGTA AGTGGGGACA GAATTCACTG CCTTGATGTC 
CTGTTTGCCT TTACAAAGCG TGTGCTAGGT GAAGGCGATG AAATGGATAA CTTAAGACAG 
CCAATGGAAG AACGTTTCAT GGCATCCAAT CCTTCAAAGG CCTCCTACGA ACCAATCACG 
TCTACTCTAC GTAGGAAACA AGAGGAGTTA GCTGCCATAG TTATCCAGCG CTGCTACAGA 
TGCTACGCTT TAAGGAAAGG CATAAAACAT GCCTCCTTTA TGTACAACCA GGAAAAGGGC 
AAAGATGGAG GGAGTCTACT TACTAGGAAA GATATGCTTA GCGATCGACT AAATGGAAAC 
TCTACAACAG AGAAAGCAGA CATTACCCCT TCGACAACGT CTCCTCCGTC CTATGACAGT 
GTTACAAAAC CAGATAAAGA AAAACACGAA AAAGACAAAA TAGAGAAGGA AGACAGGGGC 
AAAGATGTCA GGGAGCATAA AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

scn3a ( XM_031892886.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031748746.1 Xenopus tropicalis sodium channel, voltage gated, type III alpha subunit (scn3a), transcript variant X5, mRNA.

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CloneID	OXa66283
Clone ID Related Accession (Same CDS sequence)	XM_031892886.1
Accession Version	XM_031892886.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6027bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sodium channel protein type 2 subunit alpha isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGC CACTGCTACC CCCACCAGGA CCTGACAGCC TTCGACTCCT CACCAGAGAA 
TCTCTTGCTG TTCTTGAAAA GCGCTTTGCA GAAGAAAAAG CCAGGAAGGC ATCACAGAAG 
GATCGCAGAG ATGATGATGA TGAAAATGGA CCCAAGCCTA ATTCGGACTT GGAAGCTGGC 
AAAAACTTAC CATTTATATA TGGAGACATT CCACAGGGAA TGGTGGCAGT ACCAATGGAA 
GACCTGGACC CATATTACAG CAACCAAAAG ACCTTTATAG TATTGAATAG AGGGAAGGCA 
ATCTTCCGAT TCAGTGCCAC ATCTGCCTTG TACATTTTAA CTCCTTTCAA TCCTCTTAGA 
AGAATATCTA TAAAGATTTT GGTACATTCT TTATTCAGCA TGCTCATTAT GTGCACTATT 
TTGACTAATT GCTTCTTCAT GACCATGAGT AACCCACCAG AATGGACCAA GAATGTTGAA 
TATACCTTCA CTGGAATTTA TACATTTGAA TCACTTGTTA AAATACTTGC CAGAGGTTTT 
TGTTTAGAAA GCTTTACTTT TCTACGGGAT CCATGGAATT GGCTTGATTT CAGCGTCATA 
GTCATGGCGT ATGTAACAGA ATTTGTAAAC CTAGGCAATG TTTCAGCTCT TCGAACTTTC 
AGAGTATTGA GAGCTTTGAA AACTATTTCT GTAATTCCAG GTCTGAAGAC TATCGTGGGC 
GCACTGATTC AGTCGGTGAA GAAGCTTTCC GATGTAATGA TTCTCACAGT GTTTTGCCTC 
AGTGTGTTTG CACTAATAGG GCTACAGCTT TTCATGGGGC ACCTGAGGAA TAAGTGTGTA 
AGGTGGCCAC CTGAGTATGA ACCAAATATC ACTTCCTACT TTAATGAAAC TGCTGGCCTC 
TATATTAATG TAACTCTGAA CCCATTCAAT CGTGAAGAAT ACATCCTAGA AGAAACTAAT 
TTTTATTTTT TGCCTGGAAC GAGAGATGCT CTTCTGTGTG GCAACAGCTC CGATGCAGGG 
CAGTGTCCAA AAGGATATGT ATGTTTGAAG ACAGGAAGGA ACCCAAACTA CGGCTACACA 
AGTTTTGACA CATTTAGCTG GGCTTTTTTA TCACTGTTTC GGCTAATGAC TCAGGATTTC 
TGGGAAAATC TTTATCAACT GACACTACGA GCTGCTGGTA AAACCTACAT GATATTTTTC 
GTTTTGGTGA TTTTCCTGGG TTCTTTCTAT TTGATAAACT TGATCTTGGC TGTGGTGGCC 
ATGGCTTATG AAGAGCAGAA TCAGGCAACC TTAGAAGAAG CGGAGCAGAA AGAAGCAGAA 
TTCCAGCTTA TGATGGAGCA ACTGAAAAAA CATCAGGAAG AAATTCAGGC AGCGGCGCTA 
GCAGCAGAAA CAATGGACGG TTCCAGAGAG TTTAGTGGTG GGACAGGCTA TGGTGGGCTT 
TCAGAAAGCT CTTCTGAGGC ATCAAAGCCA AGTTCCAAAA GTGCTAAGGA GAGAAGAAAT 
AGAAGGAAAA AGAGAAAACA AAAAGAGCAG GGTGAGGATG AGAAGGCAGA TGATGAAAAA 
TTCCACAAGT CGGCTTCAGA AACGAGTATA AAGAGGAAAG GCTTTCGTTT TTCTATAGAA 
GGAAACCGAC TGACCTATGA GAAGAAGTAC TCGTCCCCAC ATCAGTCCCG CCTCAGTTTC 
CGTGGCTCCC TCTTTTTCCC AAGGCGTGAT AGCAGAGCGA GCCTGTGCAG CTTCAGAGGC 
CGTGGGAAGG ATGTAGGATC AGAAAATGAC TTTGCGGATG ATGAACACAG CACATTTGAG 
GACAATGAAA GCAGAAGAGG TTCATTGTTC GTTCCACACA GGCATGGAGA GAGGAGGAAC 
AGCAACATAA GTCAAACCAG CAAGGCATCA AGGACCCTGC CGGTGCTGCC AGTGAATGGG 
AAGATGCACA GCACGGTGGA TTGCAATGGA GTGGTCTCTC TACTTGGTGG CCCATCAGGT 
CCAACATCGC CTGCTGGACT ACTTCTGCCA GAGGGAACTA ATACAGAGAA TGAACTGAGG 
AAAAGACGGC TCAGTTCGTA TCATGTTTCT ATGGATTTTC TGGAAGATCC GATTCTCAGA 
CAAAGGGCTC TGAGCATTGC CAGCAATCTA ACAAATACAA TGGAAGAACT TGAAGAATCC 
AGGGAAAAAT GTCCACCATG CTGGAATAAA TTTGCCAATA CATATCTAAT CTGGGATTGC 
AGCGACTCTT GGCTAAAGAT AAAGCATGTT GTCAACTTGA TTGTCATGGA CCCATTTGTG 
GATCTTGCAA TAACAATCTG CATTGTTTTG AACACGTTAT TTATGGCTAT GGAGCATTAC 
CCAATAACCG ATGAATTTGC CGGTGCGCTC AATGTGGGAA ATTTGGTGTT TACAGGTATT 
TTTACTGCTG AGATGGTTTT CAAGCTAATT GCCCTAGATC CCTATTACTA TTTCCAAGTT 
GGCTGGAACA TATTCGATGG GGTTATTGTC AGTCTCAGCT TGATGGAACT TGGTCTTTCG 
GATGTGGAAG GGTTATCAGT CCTTCGATCT TTCAGATTGC TTCGTGTATT CAAATTGGCG 
AAATCCTGGC CTACACTGAA CATGCTGATA AAAATTATTG GCAATTCTGT AGGAGCTTTG 
GGGAACCTGA CTTTGGTGTT GGCCATCATT GTCTTCATTT TTGCTGTGGT TGGGATGCAG 
CTGTTTGGTA AAAGTTACAA GGAATGTGTC TGCAAAATTG CCCCAGATGA CTGTGAGCTT 
CCTCGCTGGC ATATGAACGA CTTCTTCCAC TCCTTCCTTA TTGTGTTCCG AGTTCTGTGT 
GGGGAATGGA TTGAAACCAT GTGGGACTGT ATGGAGGTTG CAGGACAAGC TATGTGTCTT 
ACTGTATTTA TGATGGTGAT GGTTATTGGA AACCTAGTGG TGTTAAATTT GTTTTTAGCT 
TTGCTGCTCA GCTCATTTAG CTCAGACAAT CTAGCTGCTA CAGAGGACGA TAATGAAATG 
AACAATCTAC AAATTGCAGT GGCCAGAATG CACGAAGGAA TAGCCTATGT GAAAAGGAAG 
ATGCGGGAAT TCATACAAAA CGCCTTTGTC AAAAAGCAAA AAGCCTTAGA AGAAACCAAA 
CCCTTAGAAG ACATGCAGAA CCAAAAGGAC AGCTGCATTT CCAATCACAC CACTGTGGAA 
ATAAGCAAAG AGTATGACTA CCTGAAAGAT GGAAATGTGA CAACGGCCAC CAGTGGCATA 
GGCAGCAGTG TGGAAAAGTA CATCATAGAC GAAAGCGACT ACATGTCTTT CATTCATAAC 
CCAAGCCTAA CTGTCACTGT ACCTATTGCT GTGGGGGAGT CCGATTTTGA GAATCTGAAC 
ACAGAGGAGT TTAGCAGCGA ATCAGACTTG GAAGAAAGCA AGGAGAAGCT CAACTCATCC 
AGCTCATCAG AAGGCAGTAC GGTGGACATC AGGCCTGCTG GGGAGGGAGA GCCTGTTCCA 
CAGGTGGAGG TTGAAGAGTT CCAGGAACCG GATGCTTGTT TCACAGATGG CTGTATCAGG 
AGATTTCCAT GCTGTGTGGT TGATGTCTCA CATGGCAAAT GGAAGCAGTG GTGGAACCTC 
AGAAAAACAT GTTTCTTAAT CGTAGAACAC AACTGGTTTG AGACCTTCAT AATCTTTATG 
ATTCTTATGA GTAGTGGTGC CCTGGCATTT GAAGATATCT ACATAGAACA AAGAAAAACA 
ATAAAAATAA TTTTGGAATA TGCGGACAAG GTTTTTACGT ACATCTTTAT CTTGGAAATG 
CTTCTGAAAT GGGTGGCATA TGGCTTTCAA AAATATTTCA CAAATGCCTG GTGCTGGTTG 
GACTTCTTGA TTGTTGATGT TTCTTTGGTT AGCTTAATAG CTAATGCCTT GGGCTACTCA 
GAATTAGGAG CTATTAAGTC CCTTAGGACA CTAAGGGCAT TGAGACCTCT GAGGGCCCTC 
TCACGGTTTG AAGGGATGAG GGTTGTAGTG AATGCCCTCG TTGGAGCTAT TCCATCCATT 
ATGAACGTTC TTCTGGTTTG TTTAATATTT TGGCTCATTT TTAGCATTAT GGGAGTAAAC 
ATGTTTTCCG GAAAGTATGG CTATTGTGTG AATTACACAG ATGACGTAGT GTTTGACTAC 
ACAATAGTTA ACAATAGGAG CCAATGTATG GACCTAATTA GTGAAAATAA AACGGCCAGA 
TGGAAAAACG TAAAAGTGAA TTTTGATAAC GTGGGATTTG GATATCTTGC TTTGCTTCAA 
GTGGCAACAT TCAAAGGATG GATGGAAATA ATGTATGCAG CAGTTGACTC AAGAAAGCAA 
GAGGAACAGC CAAAATATGA AGACAATCTG TACATGTATT TGTACTTCGT TATTTTCATC 
ATTTTTGGAT CGTTTTTCAC ACTCAACCTT TTCATTGGTG TCATCATTGA TAATTTTAAT 
CAGCAGAAGA AGAAGTTCGG AGGTCAAGAC ATCTTTATGA CGGAAGAACA AAAAAAATAT 
TACAATGCAA TGAAGAAGCT GGGGTCCAAG AAACCACAAA AGCCTGTTCC AAGACCTGAT 
AATAAATTTC AAGGTGCCGT CTTTGATTTT GTGTCGAAAC AACCGTTTGA TATAGCCATC 
ATGATTCTGA TCTGTTTAAA TATGGTAACC ATGATGATTG AAACAGATGA TCAGAGCATC 
GAAACGGAGA ACAACTTGTA CTGGATAAAT GCAGTTTTCA TTGTTCTTTT TACTGGGGAG 
TGTGTGCTCA AGCTGATATC CCTACGTCAT TATTACTTCA CAATTGGCTG GAACATATTT 
GATTTTGTGG TTGTAATTCT CTCTATAGTA GGTATGTTTC TGGCAGACCT TATCGAAAAA 
TATTTTGTGT CACCCACCCT GTTCAGGGTC ATCCGATTAG CTAGGATTGG TCGTATCCTG 
CGTCTCATCA AGGGAGCTAA AGGCATTCGT ACTCTGCTGT TTGCCCTGAT GATGTCACTT 
CCTGCGTTGT TCAATATTGG TTTACTGCTC TTTTTGGTGA TGTTCATCTA CGCCATTTTC 
GGCATGTCCA ACTTTGCCTA TGTTAAGAAA GAGGCTGGGA TTGATGACAT GTTTAACTTT 
GAGACCTTTG GTAACAGCAT GATCTGTTTA TTCCAAATTA CAACATCTGC AGGATGGGAT 
GGTCTGTTAG CGCCAATTCT TAATAGCGGG GCACCAGATT GCGATCCAAA GGCAGAACAT 
TCAGGAAGCA GTGTAAAAGG AGACTGTGGC AATCCCTCTG TTGGCATCTT CTTTTTTGTT 
AGTTATATCA TTATATCATT CTTGGTTGTA GTCAACATGT ATATTGCTGT CATCTTGGAA 
AACTTCAGTG TTGCTACGGA GGAAAGTGCC GAACCTCTAG GAGAAGATGA TTTTGAGATG 
TTCTATGAGG TCTGGGAAAA ATTTGATTCA AATGCAACAC AGTTTATACA ATACGTTAAA 
CTGACTGATT TTGCTGATGC GCTGGATCCG CCTCTCCGAA TGCCGAAACC AAACAATGCG 
CAGTTAATCG CAATGGATCT GCCTATGGTA AGTGGGGACA GAATTCACTG CCTTGATGTC 
CTGTTTGCCT TTACAAAGCG TGTGCTAGGT GAAGGCGATG AAATGGATAA CTTAAGACAG 
CCAATGGAAG AACGTTTCAT GGCATCCAAT CCTTCAAAGG CCTCCTACGA ACCAATCACG 
TCTACTCTAC GTAGGAAACA AGAGGAGTTA GCTGCCATAG TTATCCAGCG CTGCTACAGA 
TGCTACGCTT TAAGGAAAGG CATAAAACAT GCCTCCTTTA TGTACAACCA GGAAAAGGGC 
AAAGATGGAG GGAGTCTACT TACTAGGAAA GATATGCTTA GCGATCGACT AAATGGAAAC 
TCTACAACAG AGAAAGCAGA CATTACCCCT TCGACAACGT CTCCTCCGTC CTATGACAGT 
GTTACAAAAC CAGATAAAGA AAAACACGAA AAAGACAAAA TAGAGAAGGA AGACAGGGGC 
AAAGATGTCA GGGAGCATAA AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031748746.1
CDS	262..6288
Translation	MEQPLLPPPGPDSLRLLTRESLAVLEKRFAEEKARKASQKDRRDDDDENGPKPNSDLEAGKNLPFIYGDIPQGMVAVPMEDLDPYYSNQKTFIVLNRGKAIFRFSATSALYILTPFNPLRRISIKILVHSLFSMLIMCTILTNCFFMTMSNPPEWTKNVEYTFTGIYTFESLVKILARGFCLESFTFLRDPWNWLDFSVIVMAYVTEFVNLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGHLRNKCVRWPPEYEPNITSYFNETAGLYINVTLNPFNREEYILEETNFYFLPGTRDALLCGNSSDAGQCPKGYVCLKTGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQLMMEQLKKHQEEIQAAALAAETMDGSREFSGGTGYGGLSESSSEASKPSSKSAKERRNRRKKRKQKEQGEDEKADDEKFHKSASETSIKRKGFRFSIEGNRLTYEKKYSSPHQSRLSFRGSLFFPRRDSRASLCSFRGRGKDVGSENDFADDEHSTFEDNESRRGSLFVPHRHGERRNSNISQTSKASRTLPVLPVNGKMHSTVDCNGVVSLLGGPSGPTSPAGLLLPEGTNTENELRKRRLSSYHVSMDFLEDPILRQRALSIASNLTNTMEELEESREKCPPCWNKFANTYLIWDCSDSWLKIKHVVNLIVMDPFVDLAITICIVLNTLFMAMEHYPITDEFAGALNVGNLVFTGIFTAEMVFKLIALDPYYYFQVGWNIFDGVIVSLSLMELGLSDVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKIAPDDCELPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSSDNLAATEDDNEMNNLQIAVARMHEGIAYVKRKMREFIQNAFVKKQKALEETKPLEDMQNQKDSCISNHTTVEISKEYDYLKDGNVTTATSGIGSSVEKYIIDESDYMSFIHNPSLTVTVPIAVGESDFENLNTEEFSSESDLEESKEKLNSSSSSEGSTVDIRPAGEGEPVPQVEVEEFQEPDACFTDGCIRRFPCCVVDVSHGKWKQWWNLRKTCFLIVEHNWFETFIIFMILMSSGALAFEDIYIEQRKTIKIILEYADKVFTYIFILEMLLKWVAYGFQKYFTNAWCWLDFLIVDVSLVSLIANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNMFSGKYGYCVNYTDDVVFDYTIVNNRSQCMDLISENKTARWKNVKVNFDNVGFGYLALLQVATFKGWMEIMYAAVDSRKQEEQPKYEDNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPVPRPDNKFQGAVFDFVSKQPFDIAIMILICLNMVTMMIETDDQSIETENNLYWINAVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLADLIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKKEAGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSGAPDCDPKAEHSGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLGEDDFEMFYEVWEKFDSNATQFIQYVKLTDFADALDPPLRMPKPNNAQLIAMDLPMVSGDRIHCLDVLFAFTKRVLGEGDEMDNLRQPMEERFMASNPSKASYEPITSTLRRKQEELAAIVIQRCYRCYALRKGIKHASFMYNQEKGKDGGSLLTRKDMLSDRLNGNSTTEKADITPSTTSPPSYDSVTKPDKEKHEKDKIEKEDRGKDVREHKK

Target ORF information:

RefSeq Version	XM_031892886.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sodium channel, voltage gated, type III alpha subunit (scn3a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031892886.1

ORF Insert Sequence:

ATGGAGCAGC CACTGCTACC CCCACCAGGA CCTGACAGCC TTCGACTCCT CACCAGAGAA 
TCTCTTGCTG TTCTTGAAAA GCGCTTTGCA GAAGAAAAAG CCAGGAAGGC ATCACAGAAG 
GATCGCAGAG ATGATGATGA TGAAAATGGA CCCAAGCCTA ATTCGGACTT GGAAGCTGGC 
AAAAACTTAC CATTTATATA TGGAGACATT CCACAGGGAA TGGTGGCAGT ACCAATGGAA 
GACCTGGACC CATATTACAG CAACCAAAAG ACCTTTATAG TATTGAATAG AGGGAAGGCA 
ATCTTCCGAT TCAGTGCCAC ATCTGCCTTG TACATTTTAA CTCCTTTCAA TCCTCTTAGA 
AGAATATCTA TAAAGATTTT GGTACATTCT TTATTCAGCA TGCTCATTAT GTGCACTATT 
TTGACTAATT GCTTCTTCAT GACCATGAGT AACCCACCAG AATGGACCAA GAATGTTGAA 
TATACCTTCA CTGGAATTTA TACATTTGAA TCACTTGTTA AAATACTTGC CAGAGGTTTT 
TGTTTAGAAA GCTTTACTTT TCTACGGGAT CCATGGAATT GGCTTGATTT CAGCGTCATA 
GTCATGGCGT ATGTAACAGA ATTTGTAAAC CTAGGCAATG TTTCAGCTCT TCGAACTTTC 
AGAGTATTGA GAGCTTTGAA AACTATTTCT GTAATTCCAG GTCTGAAGAC TATCGTGGGC 
GCACTGATTC AGTCGGTGAA GAAGCTTTCC GATGTAATGA TTCTCACAGT GTTTTGCCTC 
AGTGTGTTTG CACTAATAGG GCTACAGCTT TTCATGGGGC ACCTGAGGAA TAAGTGTGTA 
AGGTGGCCAC CTGAGTATGA ACCAAATATC ACTTCCTACT TTAATGAAAC TGCTGGCCTC 
TATATTAATG TAACTCTGAA CCCATTCAAT CGTGAAGAAT ACATCCTAGA AGAAACTAAT 
TTTTATTTTT TGCCTGGAAC GAGAGATGCT CTTCTGTGTG GCAACAGCTC CGATGCAGGG 
CAGTGTCCAA AAGGATATGT ATGTTTGAAG ACAGGAAGGA ACCCAAACTA CGGCTACACA 
AGTTTTGACA CATTTAGCTG GGCTTTTTTA TCACTGTTTC GGCTAATGAC TCAGGATTTC 
TGGGAAAATC TTTATCAACT GACACTACGA GCTGCTGGTA AAACCTACAT GATATTTTTC 
GTTTTGGTGA TTTTCCTGGG TTCTTTCTAT TTGATAAACT TGATCTTGGC TGTGGTGGCC 
ATGGCTTATG AAGAGCAGAA TCAGGCAACC TTAGAAGAAG CGGAGCAGAA AGAAGCAGAA 
TTCCAGCTTA TGATGGAGCA ACTGAAAAAA CATCAGGAAG AAATTCAGGC AGCGGCGCTA 
GCAGCAGAAA CAATGGACGG TTCCAGAGAG TTTAGTGGTG GGACAGGCTA TGGTGGGCTT 
TCAGAAAGCT CTTCTGAGGC ATCAAAGCCA AGTTCCAAAA GTGCTAAGGA GAGAAGAAAT 
AGAAGGAAAA AGAGAAAACA AAAAGAGCAG GGTGAGGATG AGAAGGCAGA TGATGAAAAA 
TTCCACAAGT CGGCTTCAGA AACGAGTATA AAGAGGAAAG GCTTTCGTTT TTCTATAGAA 
GGAAACCGAC TGACCTATGA GAAGAAGTAC TCGTCCCCAC ATCAGTCCCG CCTCAGTTTC 
CGTGGCTCCC TCTTTTTCCC AAGGCGTGAT AGCAGAGCGA GCCTGTGCAG CTTCAGAGGC 
CGTGGGAAGG ATGTAGGATC AGAAAATGAC TTTGCGGATG ATGAACACAG CACATTTGAG 
GACAATGAAA GCAGAAGAGG TTCATTGTTC GTTCCACACA GGCATGGAGA GAGGAGGAAC 
AGCAACATAA GTCAAACCAG CAAGGCATCA AGGACCCTGC CGGTGCTGCC AGTGAATGGG 
AAGATGCACA GCACGGTGGA TTGCAATGGA GTGGTCTCTC TACTTGGTGG CCCATCAGGT 
CCAACATCGC CTGCTGGACT ACTTCTGCCA GAGGGAACTA ATACAGAGAA TGAACTGAGG 
AAAAGACGGC TCAGTTCGTA TCATGTTTCT ATGGATTTTC TGGAAGATCC GATTCTCAGA 
CAAAGGGCTC TGAGCATTGC CAGCAATCTA ACAAATACAA TGGAAGAACT TGAAGAATCC 
AGGGAAAAAT GTCCACCATG CTGGAATAAA TTTGCCAATA CATATCTAAT CTGGGATTGC 
AGCGACTCTT GGCTAAAGAT AAAGCATGTT GTCAACTTGA TTGTCATGGA CCCATTTGTG 
GATCTTGCAA TAACAATCTG CATTGTTTTG AACACGTTAT TTATGGCTAT GGAGCATTAC 
CCAATAACCG ATGAATTTGC CGGTGCGCTC AATGTGGGAA ATTTGGTGTT TACAGGTATT 
TTTACTGCTG AGATGGTTTT CAAGCTAATT GCCCTAGATC CCTATTACTA TTTCCAAGTT 
GGCTGGAACA TATTCGATGG GGTTATTGTC AGTCTCAGCT TGATGGAACT TGGTCTTTCG 
GATGTGGAAG GGTTATCAGT CCTTCGATCT TTCAGATTGC TTCGTGTATT CAAATTGGCG 
AAATCCTGGC CTACACTGAA CATGCTGATA AAAATTATTG GCAATTCTGT AGGAGCTTTG 
GGGAACCTGA CTTTGGTGTT GGCCATCATT GTCTTCATTT TTGCTGTGGT TGGGATGCAG 
CTGTTTGGTA AAAGTTACAA GGAATGTGTC TGCAAAATTG CCCCAGATGA CTGTGAGCTT 
CCTCGCTGGC ATATGAACGA CTTCTTCCAC TCCTTCCTTA TTGTGTTCCG AGTTCTGTGT 
GGGGAATGGA TTGAAACCAT GTGGGACTGT ATGGAGGTTG CAGGACAAGC TATGTGTCTT 
ACTGTATTTA TGATGGTGAT GGTTATTGGA AACCTAGTGG TGTTAAATTT GTTTTTAGCT 
TTGCTGCTCA GCTCATTTAG CTCAGACAAT CTAGCTGCTA CAGAGGACGA TAATGAAATG 
AACAATCTAC AAATTGCAGT GGCCAGAATG CACGAAGGAA TAGCCTATGT GAAAAGGAAG 
ATGCGGGAAT TCATACAAAA CGCCTTTGTC AAAAAGCAAA AAGCCTTAGA AGAAACCAAA 
CCCTTAGAAG ACATGCAGAA CCAAAAGGAC AGCTGCATTT CCAATCACAC CACTGTGGAA 
ATAAGCAAAG AGTATGACTA CCTGAAAGAT GGAAATGTGA CAACGGCCAC CAGTGGCATA 
GGCAGCAGTG TGGAAAAGTA CATCATAGAC GAAAGCGACT ACATGTCTTT CATTCATAAC 
CCAAGCCTAA CTGTCACTGT ACCTATTGCT GTGGGGGAGT CCGATTTTGA GAATCTGAAC 
ACAGAGGAGT TTAGCAGCGA ATCAGACTTG GAAGAAAGCA AGGAGAAGCT CAACTCATCC 
AGCTCATCAG AAGGCAGTAC GGTGGACATC AGGCCTGCTG GGGAGGGAGA GCCTGTTCCA 
CAGGTGGAGG TTGAAGAGTT CCAGGAACCG GATGCTTGTT TCACAGATGG CTGTATCAGG 
AGATTTCCAT GCTGTGTGGT TGATGTCTCA CATGGCAAAT GGAAGCAGTG GTGGAACCTC 
AGAAAAACAT GTTTCTTAAT CGTAGAACAC AACTGGTTTG AGACCTTCAT AATCTTTATG 
ATTCTTATGA GTAGTGGTGC CCTGGCATTT GAAGATATCT ACATAGAACA AAGAAAAACA 
ATAAAAATAA TTTTGGAATA TGCGGACAAG GTTTTTACGT ACATCTTTAT CTTGGAAATG 
CTTCTGAAAT GGGTGGCATA TGGCTTTCAA AAATATTTCA CAAATGCCTG GTGCTGGTTG 
GACTTCTTGA TTGTTGATGT TTCTTTGGTT AGCTTAATAG CTAATGCCTT GGGCTACTCA 
GAATTAGGAG CTATTAAGTC CCTTAGGACA CTAAGGGCAT TGAGACCTCT GAGGGCCCTC 
TCACGGTTTG AAGGGATGAG GGTTGTAGTG AATGCCCTCG TTGGAGCTAT TCCATCCATT 
ATGAACGTTC TTCTGGTTTG TTTAATATTT TGGCTCATTT TTAGCATTAT GGGAGTAAAC 
ATGTTTTCCG GAAAGTATGG CTATTGTGTG AATTACACAG ATGACGTAGT GTTTGACTAC 
ACAATAGTTA ACAATAGGAG CCAATGTATG GACCTAATTA GTGAAAATAA AACGGCCAGA 
TGGAAAAACG TAAAAGTGAA TTTTGATAAC GTGGGATTTG GATATCTTGC TTTGCTTCAA 
GTGGCAACAT TCAAAGGATG GATGGAAATA ATGTATGCAG CAGTTGACTC AAGAAAGCAA 
GAGGAACAGC CAAAATATGA AGACAATCTG TACATGTATT TGTACTTCGT TATTTTCATC 
ATTTTTGGAT CGTTTTTCAC ACTCAACCTT TTCATTGGTG TCATCATTGA TAATTTTAAT 
CAGCAGAAGA AGAAGTTCGG AGGTCAAGAC ATCTTTATGA CGGAAGAACA AAAAAAATAT 
TACAATGCAA TGAAGAAGCT GGGGTCCAAG AAACCACAAA AGCCTGTTCC AAGACCTGAT 
AATAAATTTC AAGGTGCCGT CTTTGATTTT GTGTCGAAAC AACCGTTTGA TATAGCCATC 
ATGATTCTGA TCTGTTTAAA TATGGTAACC ATGATGATTG AAACAGATGA TCAGAGCATC 
GAAACGGAGA ACAACTTGTA CTGGATAAAT GCAGTTTTCA TTGTTCTTTT TACTGGGGAG 
TGTGTGCTCA AGCTGATATC CCTACGTCAT TATTACTTCA CAATTGGCTG GAACATATTT 
GATTTTGTGG TTGTAATTCT CTCTATAGTA GGTATGTTTC TGGCAGACCT TATCGAAAAA 
TATTTTGTGT CACCCACCCT GTTCAGGGTC ATCCGATTAG CTAGGATTGG TCGTATCCTG 
CGTCTCATCA AGGGAGCTAA AGGCATTCGT ACTCTGCTGT TTGCCCTGAT GATGTCACTT 
CCTGCGTTGT TCAATATTGG TTTACTGCTC TTTTTGGTGA TGTTCATCTA CGCCATTTTC 
GGCATGTCCA ACTTTGCCTA TGTTAAGAAA GAGGCTGGGA TTGATGACAT GTTTAACTTT 
GAGACCTTTG GTAACAGCAT GATCTGTTTA TTCCAAATTA CAACATCTGC AGGATGGGAT 
GGTCTGTTAG CGCCAATTCT TAATAGCGGG GCACCAGATT GCGATCCAAA GGCAGAACAT 
TCAGGAAGCA GTGTAAAAGG AGACTGTGGC AATCCCTCTG TTGGCATCTT CTTTTTTGTT 
AGTTATATCA TTATATCATT CTTGGTTGTA GTCAACATGT ATATTGCTGT CATCTTGGAA 
AACTTCAGTG TTGCTACGGA GGAAAGTGCC GAACCTCTAG GAGAAGATGA TTTTGAGATG 
TTCTATGAGG TCTGGGAAAA ATTTGATTCA AATGCAACAC AGTTTATACA ATACGTTAAA 
CTGACTGATT TTGCTGATGC GCTGGATCCG CCTCTCCGAA TGCCGAAACC AAACAATGCG 
CAGTTAATCG CAATGGATCT GCCTATGGTA AGTGGGGACA GAATTCACTG CCTTGATGTC 
CTGTTTGCCT TTACAAAGCG TGTGCTAGGT GAAGGCGATG AAATGGATAA CTTAAGACAG 
CCAATGGAAG AACGTTTCAT GGCATCCAAT CCTTCAAAGG CCTCCTACGA ACCAATCACG 
TCTACTCTAC GTAGGAAACA AGAGGAGTTA GCTGCCATAG TTATCCAGCG CTGCTACAGA 
TGCTACGCTT TAAGGAAAGG CATAAAACAT GCCTCCTTTA TGTACAACCA GGAAAAGGGC 
AAAGATGGAG GGAGTCTACT TACTAGGAAA GATATGCTTA GCGATCGACT AAATGGAAAC 
TCTACAACAG AGAAAGCAGA CATTACCCCT TCGACAACGT CTCCTCCGTC CTATGACAGT 
GTTACAAAAC CAGATAAAGA AAAACACGAA AAAGACAAAA TAGAGAAGGA AGACAGGGGC 
AAAGATGTCA GGGAGCATAA AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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scn3a cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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