otof cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	otof
Entrez Gene ID	100125064
Full Name	otoferlin
General protein information	Preferred Names otoferlin Names otoferlin
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031901883.1	XP_031757743.1	otoferlin isoform X4
XM_031901881.1	XP_031757741.1	otoferlin isoform X2
XM_031901880.1	XP_031757740.1	otoferlin isoform X1
XM_031901885.1	XP_031757745.1	otoferlin isoform X6
XM_012971322.1	XP_012826776.1	otoferlin
XM_031901882.1	XP_031757742.1	otoferlin isoform X3
XM_031901884.1	XP_031757744.1	otoferlin isoform X5

Pathways from BioSystems

Homologies

Homo sapiens (human)	OTOF	NP_919224.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000389	XP_310715.5
Canis lupus familiaris (dog)	OTOF	XP_005630285.1
Bos taurus (cattle)	OTOF	NP_001137579.1
Xenopus tropicalis (tropical clawed frog)	otof	XP_004918245.1
Mus musculus (house mouse)	Otof	NP_114081.2
Gallus gallus (chicken)	OTOF	XP_420015.2
Danio rerio (zebrafish)	otof	NP_001025283.1
Pan troglodytes (chimpanzee)	OTOF	XP_003949828.1
Macaca mulatta (Rhesus monkey)	OTOF	XP_002799220.1
Rattus norvegicus (Norway rat)	Otof	NP_001263649.1

Gene Ontology
Bibliography

Related articles in PubMed

Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
Strausberg RL, Feingold EA, Grouse LH, Derge JG, Klausner RD, Collins FS, Wagner L, Shenmen CM, Schuler GD, Altschul SF, Zeeberg B, Buetow KH, Schaefer CF, Bhat NK, Hopkins RF, Jordan H, Moore T, Max SI, Wang J, Hsieh F, Diatchenko L, Marusina K, Farmer AA, Rubin GM, Hong L, Stapleton M, Soares MB, Bonaldo MF, Casavant TL, Scheetz TE, Brownstein MJ, Usdin TB, Toshiyuki S, Carninci P, Prange C, Raha SS, Loquellano NA, Peters GJ, Abramson RD, Mullahy SJ, Bosak SA, McEwan PJ, McKernan KJ, Malek JA, Gunaratne PH, Richards S, Worley KC, Hale S, Garcia AM, Gay LJ, Hulyk SW, Villalon DK, Muzny DM, Sodergren EJ, Lu X, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madan A, Young AC, Shevchenko Y, Bouffard GG, Blakesley RW, Touchman JW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Krzywinski MI, Skalska U, Smailus DE, Schnerch A, Schein JE, Jones SJ, Marra MA,
Proceedings of the National Academy of Sciences of the United States of America99(26)16899-903(2002 Dec)

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following otof gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the otof cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa58168	XM_031901880.1 Latest version!	Xenopus tropicalis otoferlin (otof), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa58169	XM_031901881.1 Latest version!	Xenopus tropicalis otoferlin (otof), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa58170	XM_031901882.1 Latest version!	Xenopus tropicalis otoferlin (otof), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa58171	XM_031901883.1 Latest version!	Xenopus tropicalis otoferlin (otof), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa58172	XM_031901884.1 Latest version!	Xenopus tropicalis otoferlin (otof), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa58173	XM_031901885.1 Latest version!	Xenopus tropicalis otoferlin (otof), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa30655	XM_012971322.1 Latest version!	Xenopus tropicalis otoferlin (otof), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

otof ( XM_031901880.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757740.1 Xenopus tropicalis otoferlin (otof), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa58168
Clone ID Related Accession (Same CDS sequence)	XM_031901880.1
Accession Version	XM_031901880.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4740bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	otoferlin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACACCA GCATCATGGC CAACGTCAAG AAAGCTCTCA TTGGAGAAAA CAAGGACTTG 
GTGGACCCCT ATGTGCAAGT TGCCTTTGCA GGGCAGAAGG GAAAGACCTC GGTGCAGAAG 
AGCAGCTACG AGCCCATGTG GAACGAGCAG ATCATTTTCA CCGAGATGTT CCCCCCCCTG 
TGCAAGAGGA TGAAAGTGCA GATCAGAGAT TCCGACAAGG TCAACGATGT CGCCATTGGG 
ACCCACTTCA TTGACCTGAG GAAAATTGCC AACGAGGGGG ACAAGGGATT CCTCCCTACT 
TTCGGCCCAG CCTGGGTGAA TATGTATGGC TCCACCCGTA ACTACACCCT CATGGATGAG 
CACCAGGATC TGAATGAGGG GCTCGGAGAA GGCGTCTCTT TCCGCGCAAG GCTGCTGCTG 
AGTCTCGCTG TTGAGATACT GGATACAACC AACCCAGAGC TCACCAGTTC CACAGAGGTT 
CAAGTTGAAG GAGCTCAACC AGTGGCAGAA ACCTGTACCG GTAAAATGGA AGAGTTCTTC 
CTGTTTGGAG CATTCCTGGA ATCCACCATG ATTGATCGGA AAATTGGGGA CAAGCCAATT 
AACTTTGAGG TCACCATAGG TAACTATGGC AACCAAATAG ATGGAATTTC CAAGCCGAGT 
ATGAGGAAGA GGAAGGAAGG AGGTGACGGA GTCGAGGAGG AGACGGAGCT CTTGCAGAAT 
TCCAGTGATG ATGAGGCTGA TGAAGATGGA GAAATGTTAT CAATCTCTTC GTCCCCTGCA 
ATGAAGCCAC TCATAACTGA TAGGAACTAC TTCCACCTCC CGTTCTTTGA TAAGAAGCCC 
TGTACCTACA TTAAGAGTTG GTGGCAGGAC CAGCGGAGGC GGCTGTACAA TGCCAACATT 
ATGGACAAGA TCGCAGATAA GCTGGAGGAA GGCTTGAACG ATGTTCATGA GATGATAAAG 
ACGGAGAAGC CCTACCCTGA ACGGCGGCTG CGAGGAGTCC TGGAGGAGCT GAGTAGCAGC 
TGTTTTCGAT TTGTTACCTT GGCCAACAAG GACCAGAACC AGAATGGGCA GACGAAACTG 
GACAGGGAGA GGCTGAAGTC CTGCATGAGG GAGCTGGAGA ACATGGGGAC ACAAGCCAAG 
ACCATGAGGT CCCAAGTGAA GAAGAACACG GTTAAGGACA AGCTGAAACT GGTGCAGAAT 
TTCCTGCAGA AGCTCCGGTT CCTGGCAGAT GAGCCCCAGC ACAGCATTCC TGACATCTTC 
ATCTGGATGA TGTCCAACAA CAAGCGCATC GCCTACGCTC GAATCCCTTC CAAAGATATC 
CTGTACTCCA TTGTGGATGA GGAGACGGGA AAGGACTGTG GCAAAGTCAA GTGTGTCTTC 
CTTAAGCTGC CGGGCAAGAG GGGCTTTGGG CCTGCAGGCT GGACGGTACA AGCCAAGATG 
GAGGTCTACA TGTGGTTGGG CCTTAGCAAG CAGCGCAGGG ATTTCTTGTG TGGTCTTCCA 
TGTGGATTTG AGGAGATAAA AGCAGTGAAA GGGCCCGGTC TGCAGTCCTT CCCACCCATC 
AGCCTCAGCT ACACCAAAAA ACAAGTGTTC CAGTTACGAG CCCACATGTA CCAAGCCCGG 
AGTCTCTTTG CCGCTGACAG CAGTGGACTT TCCGACCCCT TTGCCCGCGT CTTCTTCAAC 
ACTCAGAGCC AATGTACAGA GGTGCTGAAT GAGACCCTTT GTCCGACTTG GGACCAGCTG 
TTGGTGTTTG ACAATATCGA ACTCTACGGA GAAGCTCACG AGATGAGAGA CGACCCCCCA 
ATCATTGTCA TTGAGATTTA TGATCAAGAC ACAGTAGGGA AGGCCGATTT CATGGGGAGA 
ACATTTGCCA AACCTGTTGT GAAAATGTCA GATGAGCGGT ACTGCCCTCC CCGCTTTCCG 
CCACAGCTGG AATACTACCA GATCTACCGT GGGAACTCAA CCGCAGGGGA CCTCTTGGGA 
GCTTTCGAAC TTCTCCAGAT CGGACCAGCG GGCAAGTCTG ACCTCCCACA GATTGAAGGA 
CCAACAGACA TAGAGAGAGG ACCCATCCTT CCAGTTCCTC TTGGAATCAG ACCAGTGCTC 
AGCAAATACA GAGTAGAGGT CCTTTTCTGG GGGCTGAGAG ACCTGAAGAG AGTAAATCTA 
GCCCAGGTTG ATAGACCAAG GGTGGATATT GAGTGTGCCG GGAGGGGGGT GCAGTCTGCC 
CTCATCCAGA ACTACAAGAA GAATCCAAAT TTTAGCACGT TGGTCAAATG GTTTGAAGTG 
GATCTCCCCG AGAATGAGTT ACTTCACCCG CCATTGAATA TCCGAGTAGT AGACTGCCGA 
GCCTTTGGCA GATACACTCT GGTTGGTTCC CATGCTGTCA GCTCCCTACG CAGGTTCATT 
TACCGGCCTC CGGATAAGAA CTCCCAGCAG TGGACCACAG CAGCTAAACT CATGAATGGC 
TACTTGGCAA TGACCAATGG AAGGCCCCGC TCTCGCACCA CAGGTGAAAT AGTTATCAAC 
ATGGAGCCCG AAGCGCCCGT CAAGAAGATG GAGACAATGG TGAAACTGGA AGCCAATTCC 
GACGCGGTGG TTAAAGTAGA CGTGAGCGAG GAAGAAAAAG ACAAGAAGAA GAAGAAGAAG 
AAAGGAGTAG AGGAGATAGA GGAAGAGGAG CCAGATGAGA GCATGTTGGA CTGGTGGTCC 
AAATACTTTG CTTCCATTGA AACCATGAAG GAGCAACTGC GCCAACAGGA AGCGGCAGCC 
GCCGAAGCCG AAGAGAAGGA AGAGATGGAG ATCGCAGAGG AGATCAAAAC TGATGACTCT 
CCCATGAAAG GCAACAAGGG CCCCACGAAG AACAAGGAGA AGGTCAAAGC CCCAAAAGAG 
GAGAAGAAGA ACAAGAAGCA GCAACAGCAG CAGCAACAAG CCGATCAGGC CGAGAAGAAG 
AACAAGCAAA AGATCGATGA GTTAAAGGTG TACAACAAGG AGTTGGAGAC CGAGTATGAC 
AACTTTGAGG ACTGGCTCCA TACATTTAAC TTGTTACGAG GGAAGATTGG AGATGATGAC 
GATACTGTAA GCGAAGAAGA GCGAATTGTT GGGAGGTTTA AGGGTTCCAT GTGTGTGTAC 
AAAGTTCCTT TGCCAGAAGA CGTCACAAAG GAAGCCGGGT ATGACCCAAA CTTCGGCATG 
TTCCAAGGAA TCCCTAACAA CGACCCAATG AATGTGCTGG TCAGAGTTTA TGTCGTGAGG 
GCCACCGATC TTCATCCAGC TGATATCAAT GGTAAAGCGG ATCCATATGT TGTCATAAAA 
CTAGGAAAAA CTGATATAAA AGATAAGGAA AACTACATCT CCAAGCAGCT AAACCCTGTC 
TTCGGAAAGT CCTTTGATAT TGAGGCCACT TTTCCGATGG AGTCCATGCT TACAGTTGCG 
GTTTATGACT GGGATCTGGT GGGGACGGAT GATCTGATCG GTGAAACCAA AATCGACCTG 
GAAAACCGTT ACTACAGCAA GCACAGGGCG ACTTGTGGTA TCTCAAAGAC ATATGCCATT 
CACGGGTACA ACTTGTGGAG GGACCCAGTG AAGCCTACAC AAATCTTATC CAAACTTTGC 
AAAGAAGGGA AGATCGATGG ACCTCATTAT GGCCCTGGTG GAAGAGTGAA AGTCACGAAT 
CGGGTATTCA CTGGTCCCAC AGAAATTGAT GATGAAAATG GTCAGAAGAA ACAAACGGAT 
GAGCACCTTG CACTCTCGGT CCTTCAGCAC TGGGAGGACA TTCCTCGGGT GGGATGTAAG 
CTGGTCCCAG AGCATGTAGA AACGAGGCCT TTGTTAAACC CAGACAAACC TGGAATTGAG 
CAGGGCCGCA TAGAAATGTG GGTAGACATG TTCCCCATGG ACATGCCTGC GCCAGGTCCT 
GCCATTGATA TCTCTCCAAG GAAGCCAAAA AGTTTTGAGC TACGTGTTAT TATTTGGAAC 
ACTGATGAAG TGATTCTAGA AGATGATGCT TTCATGACAG GAGAGAAGAT GTCTGACATC 
TATGTCAAGG GGTGGCTGAA GGGACAGCAG GAAGACAAAC AAGACACGGA TGTCCACTAT 
CATTCCCTTA CTGGGGAGGG GAACTTCAAC TGGAGGTTCT TGTTCCCTTT TGATTACCTT 
ATGGCAGAGG AGAAGATTGT CATCTCCAAG AAAGAGTCCA TGTTCTCTTG GGATGAGACC 
GAGTACAAGA TCCCGGCTCG TCTCACTCTC CAGGTCTGGG ATGCCGACCA CTTCTCAGCA 
GATGACTTTC TCGGAGCCAT AGAATTAGAC TTGAATCGCT TTCCCCGAGG GGCAAAAACA 
GCGAAACAGT GTTCGATAGA AATGGCGGAA CCCGACGTCG ATCTGCCAAT GGTCTCAATC 
TTCAAACAGA AGAGGGTGAA GGGATGGTGG CCTTTCGTTG CCAGGAATGA AAACGATGAG 
ATGGAGCTGA CTGGTAAAGT GGAGGCGGAG CTTCACCTTC TCTCAGCAGA GGAGGCAGAG 
AAGACCCCGG CAGGGCTTGG GCGCAATGAA CCAGATCCTC TTGAAAAACC AAATCGGCCG 
AATCCCAGCC TGGCGTGGAT GCTGAGCCCC CTGCGGGTAG TGTGCATGTT GCTATGGCGA 
CAGTACCGGT GGCACGTACT GGGCATCATC GGTTCCTGTG CGGTTCTGAT GTTCGTGGCG 
TTGGTGCTCT ACACCCTCCC CGGCGCTATC AGCGAGAGGC TCATTAACCC CCGCGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757740.1
CDS	97..4836
Translation	MNTSIMANVKKALIGENKDLVDPYVQVAFAGQKGKTSVQKSSYEPMWNEQIIFTEMFPPLCKRMKVQIRDSDKVNDVAIGTHFIDLRKIANEGDKGFLPTFGPAWVNMYGSTRNYTLMDEHQDLNEGLGEGVSFRARLLLSLAVEILDTTNPELTSSTEVQVEGAQPVAETCTGKMEEFFLFGAFLESTMIDRKIGDKPINFEVTIGNYGNQIDGISKPSMRKRKEGGDGVEEETELLQNSSDDEADEDGEMLSISSSPAMKPLITDRNYFHLPFFDKKPCTYIKSWWQDQRRRLYNANIMDKIADKLEEGLNDVHEMIKTEKPYPERRLRGVLEELSSSCFRFVTLANKDQNQNGQTKLDRERLKSCMRELENMGTQAKTMRSQVKKNTVKDKLKLVQNFLQKLRFLADEPQHSIPDIFIWMMSNNKRIAYARIPSKDILYSIVDEETGKDCGKVKCVFLKLPGKRGFGPAGWTVQAKMEVYMWLGLSKQRRDFLCGLPCGFEEIKAVKGPGLQSFPPISLSYTKKQVFQLRAHMYQARSLFAADSSGLSDPFARVFFNTQSQCTEVLNETLCPTWDQLLVFDNIELYGEAHEMRDDPPIIVIEIYDQDTVGKADFMGRTFAKPVVKMSDERYCPPRFPPQLEYYQIYRGNSTAGDLLGAFELLQIGPAGKSDLPQIEGPTDIERGPILPVPLGIRPVLSKYRVEVLFWGLRDLKRVNLAQVDRPRVDIECAGRGVQSALIQNYKKNPNFSTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHAVSSLRRFIYRPPDKNSQQWTTAAKLMNGYLAMTNGRPRSRTTGEIVINMEPEAPVKKMETMVKLEANSDAVVKVDVSEEEKDKKKKKKKGVEEIEEEEPDESMLDWWSKYFASIETMKEQLRQQEAAAAEAEEKEEMEIAEEIKTDDSPMKGNKGPTKNKEKVKAPKEEKKNKKQQQQQQQADQAEKKNKQKIDELKVYNKELETEYDNFEDWLHTFNLLRGKIGDDDDTVSEEERIVGRFKGSMCVYKVPLPEDVTKEAGYDPNFGMFQGIPNNDPMNVLVRVYVVRATDLHPADINGKADPYVVIKLGKTDIKDKENYISKQLNPVFGKSFDIEATFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRYYSKHRATCGISKTYAIHGYNLWRDPVKPTQILSKLCKEGKIDGPHYGPGGRVKVTNRVFTGPTEIDDENGQKKQTDEHLALSVLQHWEDIPRVGCKLVPEHVETRPLLNPDKPGIEQGRIEMWVDMFPMDMPAPGPAIDISPRKPKSFELRVIIWNTDEVILEDDAFMTGEKMSDIYVKGWLKGQQEDKQDTDVHYHSLTGEGNFNWRFLFPFDYLMAEEKIVISKKESMFSWDETEYKIPARLTLQVWDADHFSADDFLGAIELDLNRFPRGAKTAKQCSIEMAEPDVDLPMVSIFKQKRVKGWWPFVARNENDEMELTGKVEAELHLLSAEEAEKTPAGLGRNEPDPLEKPNRPNPSLAWMLSPLRVVCMLLWRQYRWHVLGIIGSCAVLMFVALVLYTLPGAISERLINPRG

Target ORF information:

RefSeq Version	XM_031901880.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis otoferlin (otof), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901880.1

ORF Insert Sequence:

ATGAACACCA GCATCATGGC CAACGTCAAG AAAGCTCTCA TTGGAGAAAA CAAGGACTTG 
GTGGACCCCT ATGTGCAAGT TGCCTTTGCA GGGCAGAAGG GAAAGACCTC GGTGCAGAAG 
AGCAGCTACG AGCCCATGTG GAACGAGCAG ATCATTTTCA CCGAGATGTT CCCCCCCCTG 
TGCAAGAGGA TGAAAGTGCA GATCAGAGAT TCCGACAAGG TCAACGATGT CGCCATTGGG 
ACCCACTTCA TTGACCTGAG GAAAATTGCC AACGAGGGGG ACAAGGGATT CCTCCCTACT 
TTCGGCCCAG CCTGGGTGAA TATGTATGGC TCCACCCGTA ACTACACCCT CATGGATGAG 
CACCAGGATC TGAATGAGGG GCTCGGAGAA GGCGTCTCTT TCCGCGCAAG GCTGCTGCTG 
AGTCTCGCTG TTGAGATACT GGATACAACC AACCCAGAGC TCACCAGTTC CACAGAGGTT 
CAAGTTGAAG GAGCTCAACC AGTGGCAGAA ACCTGTACCG GTAAAATGGA AGAGTTCTTC 
CTGTTTGGAG CATTCCTGGA ATCCACCATG ATTGATCGGA AAATTGGGGA CAAGCCAATT 
AACTTTGAGG TCACCATAGG TAACTATGGC AACCAAATAG ATGGAATTTC CAAGCCGAGT 
ATGAGGAAGA GGAAGGAAGG AGGTGACGGA GTCGAGGAGG AGACGGAGCT CTTGCAGAAT 
TCCAGTGATG ATGAGGCTGA TGAAGATGGA GAAATGTTAT CAATCTCTTC GTCCCCTGCA 
ATGAAGCCAC TCATAACTGA TAGGAACTAC TTCCACCTCC CGTTCTTTGA TAAGAAGCCC 
TGTACCTACA TTAAGAGTTG GTGGCAGGAC CAGCGGAGGC GGCTGTACAA TGCCAACATT 
ATGGACAAGA TCGCAGATAA GCTGGAGGAA GGCTTGAACG ATGTTCATGA GATGATAAAG 
ACGGAGAAGC CCTACCCTGA ACGGCGGCTG CGAGGAGTCC TGGAGGAGCT GAGTAGCAGC 
TGTTTTCGAT TTGTTACCTT GGCCAACAAG GACCAGAACC AGAATGGGCA GACGAAACTG 
GACAGGGAGA GGCTGAAGTC CTGCATGAGG GAGCTGGAGA ACATGGGGAC ACAAGCCAAG 
ACCATGAGGT CCCAAGTGAA GAAGAACACG GTTAAGGACA AGCTGAAACT GGTGCAGAAT 
TTCCTGCAGA AGCTCCGGTT CCTGGCAGAT GAGCCCCAGC ACAGCATTCC TGACATCTTC 
ATCTGGATGA TGTCCAACAA CAAGCGCATC GCCTACGCTC GAATCCCTTC CAAAGATATC 
CTGTACTCCA TTGTGGATGA GGAGACGGGA AAGGACTGTG GCAAAGTCAA GTGTGTCTTC 
CTTAAGCTGC CGGGCAAGAG GGGCTTTGGG CCTGCAGGCT GGACGGTACA AGCCAAGATG 
GAGGTCTACA TGTGGTTGGG CCTTAGCAAG CAGCGCAGGG ATTTCTTGTG TGGTCTTCCA 
TGTGGATTTG AGGAGATAAA AGCAGTGAAA GGGCCCGGTC TGCAGTCCTT CCCACCCATC 
AGCCTCAGCT ACACCAAAAA ACAAGTGTTC CAGTTACGAG CCCACATGTA CCAAGCCCGG 
AGTCTCTTTG CCGCTGACAG CAGTGGACTT TCCGACCCCT TTGCCCGCGT CTTCTTCAAC 
ACTCAGAGCC AATGTACAGA GGTGCTGAAT GAGACCCTTT GTCCGACTTG GGACCAGCTG 
TTGGTGTTTG ACAATATCGA ACTCTACGGA GAAGCTCACG AGATGAGAGA CGACCCCCCA 
ATCATTGTCA TTGAGATTTA TGATCAAGAC ACAGTAGGGA AGGCCGATTT CATGGGGAGA 
ACATTTGCCA AACCTGTTGT GAAAATGTCA GATGAGCGGT ACTGCCCTCC CCGCTTTCCG 
CCACAGCTGG AATACTACCA GATCTACCGT GGGAACTCAA CCGCAGGGGA CCTCTTGGGA 
GCTTTCGAAC TTCTCCAGAT CGGACCAGCG GGCAAGTCTG ACCTCCCACA GATTGAAGGA 
CCAACAGACA TAGAGAGAGG ACCCATCCTT CCAGTTCCTC TTGGAATCAG ACCAGTGCTC 
AGCAAATACA GAGTAGAGGT CCTTTTCTGG GGGCTGAGAG ACCTGAAGAG AGTAAATCTA 
GCCCAGGTTG ATAGACCAAG GGTGGATATT GAGTGTGCCG GGAGGGGGGT GCAGTCTGCC 
CTCATCCAGA ACTACAAGAA GAATCCAAAT TTTAGCACGT TGGTCAAATG GTTTGAAGTG 
GATCTCCCCG AGAATGAGTT ACTTCACCCG CCATTGAATA TCCGAGTAGT AGACTGCCGA 
GCCTTTGGCA GATACACTCT GGTTGGTTCC CATGCTGTCA GCTCCCTACG CAGGTTCATT 
TACCGGCCTC CGGATAAGAA CTCCCAGCAG TGGACCACAG CAGCTAAACT CATGAATGGC 
TACTTGGCAA TGACCAATGG AAGGCCCCGC TCTCGCACCA CAGGTGAAAT AGTTATCAAC 
ATGGAGCCCG AAGCGCCCGT CAAGAAGATG GAGACAATGG TGAAACTGGA AGCCAATTCC 
GACGCGGTGG TTAAAGTAGA CGTGAGCGAG GAAGAAAAAG ACAAGAAGAA GAAGAAGAAG 
AAAGGAGTAG AGGAGATAGA GGAAGAGGAG CCAGATGAGA GCATGTTGGA CTGGTGGTCC 
AAATACTTTG CTTCCATTGA AACCATGAAG GAGCAACTGC GCCAACAGGA AGCGGCAGCC 
GCCGAAGCCG AAGAGAAGGA AGAGATGGAG ATCGCAGAGG AGATCAAAAC TGATGACTCT 
CCCATGAAAG GCAACAAGGG CCCCACGAAG AACAAGGAGA AGGTCAAAGC CCCAAAAGAG 
GAGAAGAAGA ACAAGAAGCA GCAACAGCAG CAGCAACAAG CCGATCAGGC CGAGAAGAAG 
AACAAGCAAA AGATCGATGA GTTAAAGGTG TACAACAAGG AGTTGGAGAC CGAGTATGAC 
AACTTTGAGG ACTGGCTCCA TACATTTAAC TTGTTACGAG GGAAGATTGG AGATGATGAC 
GATACTGTAA GCGAAGAAGA GCGAATTGTT GGGAGGTTTA AGGGTTCCAT GTGTGTGTAC 
AAAGTTCCTT TGCCAGAAGA CGTCACAAAG GAAGCCGGGT ATGACCCAAA CTTCGGCATG 
TTCCAAGGAA TCCCTAACAA CGACCCAATG AATGTGCTGG TCAGAGTTTA TGTCGTGAGG 
GCCACCGATC TTCATCCAGC TGATATCAAT GGTAAAGCGG ATCCATATGT TGTCATAAAA 
CTAGGAAAAA CTGATATAAA AGATAAGGAA AACTACATCT CCAAGCAGCT AAACCCTGTC 
TTCGGAAAGT CCTTTGATAT TGAGGCCACT TTTCCGATGG AGTCCATGCT TACAGTTGCG 
GTTTATGACT GGGATCTGGT GGGGACGGAT GATCTGATCG GTGAAACCAA AATCGACCTG 
GAAAACCGTT ACTACAGCAA GCACAGGGCG ACTTGTGGTA TCTCAAAGAC ATATGCCATT 
CACGGGTACA ACTTGTGGAG GGACCCAGTG AAGCCTACAC AAATCTTATC CAAACTTTGC 
AAAGAAGGGA AGATCGATGG ACCTCATTAT GGCCCTGGTG GAAGAGTGAA AGTCACGAAT 
CGGGTATTCA CTGGTCCCAC AGAAATTGAT GATGAAAATG GTCAGAAGAA ACAAACGGAT 
GAGCACCTTG CACTCTCGGT CCTTCAGCAC TGGGAGGACA TTCCTCGGGT GGGATGTAAG 
CTGGTCCCAG AGCATGTAGA AACGAGGCCT TTGTTAAACC CAGACAAACC TGGAATTGAG 
CAGGGCCGCA TAGAAATGTG GGTAGACATG TTCCCCATGG ACATGCCTGC GCCAGGTCCT 
GCCATTGATA TCTCTCCAAG GAAGCCAAAA AGTTTTGAGC TACGTGTTAT TATTTGGAAC 
ACTGATGAAG TGATTCTAGA AGATGATGCT TTCATGACAG GAGAGAAGAT GTCTGACATC 
TATGTCAAGG GGTGGCTGAA GGGACAGCAG GAAGACAAAC AAGACACGGA TGTCCACTAT 
CATTCCCTTA CTGGGGAGGG GAACTTCAAC TGGAGGTTCT TGTTCCCTTT TGATTACCTT 
ATGGCAGAGG AGAAGATTGT CATCTCCAAG AAAGAGTCCA TGTTCTCTTG GGATGAGACC 
GAGTACAAGA TCCCGGCTCG TCTCACTCTC CAGGTCTGGG ATGCCGACCA CTTCTCAGCA 
GATGACTTTC TCGGAGCCAT AGAATTAGAC TTGAATCGCT TTCCCCGAGG GGCAAAAACA 
GCGAAACAGT GTTCGATAGA AATGGCGGAA CCCGACGTCG ATCTGCCAAT GGTCTCAATC 
TTCAAACAGA AGAGGGTGAA GGGATGGTGG CCTTTCGTTG CCAGGAATGA AAACGATGAG 
ATGGAGCTGA CTGGTAAAGT GGAGGCGGAG CTTCACCTTC TCTCAGCAGA GGAGGCAGAG 
AAGACCCCGG CAGGGCTTGG GCGCAATGAA CCAGATCCTC TTGAAAAACC AAATCGGCCG 
AATCCCAGCC TGGCGTGGAT GCTGAGCCCC CTGCGGGTAG TGTGCATGTT GCTATGGCGA 
CAGTACCGGT GGCACGTACT GGGCATCATC GGTTCCTGTG CGGTTCTGAT GTTCGTGGCG 
TTGGTGCTCT ACACCCTCCC CGGCGCTATC AGCGAGAGGC TCATTAACCC CCGCGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

otof ( XM_031901881.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757741.1 Xenopus tropicalis otoferlin (otof), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa58169
Clone ID Related Accession (Same CDS sequence)	XM_031901881.1
Accession Version	XM_031901881.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4734bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	otoferlin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACACCA GCATCATGGC CAACGTCAAG AAAGCTCTCA TTGGAGAAAA CAAGGACTTG 
GTGGACCCCT ATGTGCAAGT TGCCTTTGCA GGGCAGAAGG GAAAGACCTC GGTGCAGAAG 
AGCAGCTACG AGCCCATGTG GAACGAGCAG ATCATTTTCA CCGAGATGTT CCCCCCCCTG 
TGCAAGAGGA TGAAAGTGCA GATCAGAGAT TCCGACAAGG TCAACGATGT CGCCATTGGG 
ACCCACTTCA TTGACCTGAG GAAAATTGCC AACGAGGGGG ACAAGGGATT CCTCCCTACT 
TTCGGCCCAG CCTGGGTGAA TATGTATGGC TCCACCCGTA ACTACACCCT CATGGATGAG 
CACCAGGATC TGAATGAGGG GCTCGGAGAA GGCGTCTCTT TCCGCGCAAG GCTGCTGCTG 
AGTCTCGCTG TTGAGATACT GGATACAACC AACCCAGAGC TCACCAGTTC CACAGAGGTT 
CAAGTTGAAG GAGCTCAACC AGTGGCAGAA ACCTGTACCG GTAAAATGGA AGAGTTCTTC 
CTGTTTGGAG CATTCCTGGA ATCCACCATG ATTGATCGGA AAATTGGGGA CAAGCCAATT 
AACTTTGAGG TCACCATAGG TAACTATGGC AACCAAATAG ATGGAATTTC CAAGCCGAGT 
ATGAGGAAGA GGAAGGAAGG AGGTGACGGA GTCGAGGAGG AGACGGAGCT CTTGCAGAAT 
TCCAGTGATG ATGAGGCTGA TGAAGATGGA GAAATGTTAT CAATCTCTTC GTCCCCTGCA 
ATGAAGCCAC TCATAACTGA TAGGAACTAC TTCCACCTCC CGTTCTTTGA TAAGAAGCCC 
TGTACCTACA TTAAGAGTTG GTGGCAGGAC CAGCGGAGGC GGCTGTACAA TGCCAACATT 
ATGGACAAGA TCGCAGATAA GCTGGAGGAA GGCTTGAACG ATGTTCATGA GATGATAAAG 
ACGGAGAAGC CCTACCCTGA ACGGCGGCTG CGAGGAGTCC TGGAGGAGCT GAGTAGCAGC 
TGTTTTCGAT TTGTTACCTT GGCCAACAAG GACCAGAACC AGAATGGGCA GACGAAACTG 
GACAGGGAGA GGCTGAAGTC CTGCATGAGG GAGCTGGAGA ACATGGGGAC ACAAGCCAAG 
ACCATGAGGT CCCAAGTGAA GAAGAACACG GTTAAGGACA AGCTGAAACT GGTGCAGAAT 
TTCCTGCAGA AGCTCCGGTT CCTGGCAGAT GAGCCCCAGC ACAGCATTCC TGACATCTTC 
ATCTGGATGA TGTCCAACAA CAAGCGCATC GCCTACGCTC GAATCCCTTC CAAAGATATC 
CTGTACTCCA TTGTGGATGA GGAGACGGGA AAGGACTGTG GCAAAGTCAA GTGTGTCTTC 
CTTAAGCTGC CGGGCAAGAG GGGCTTTGGG CCTGCAGGCT GGACGGTACA AGCCAAGATG 
GAGGTCTACA TGTGGTTGGG CCTTAGCAAG CAGCGCAGGG ATTTCTTGTG TGGTCTTCCA 
TGTGGATTTG AGGAGATAAA AGCAGTGAAA GGGCCCGGTC TGCAGTCCTT CCCACCCATC 
AGCCTCAGCT ACACCAAAAA ACAAGTGTTC CAGTTACGAG CCCACATGTA CCAAGCCCGG 
AGTCTCTTTG CCGCTGACAG CAGTGGACTT TCCGACCCCT TTGCCCGCGT CTTCTTCAAC 
ACTCAGAGCC AATGTACAGA GGTGCTGAAT GAGACCCTTT GTCCGACTTG GGACCAGCTG 
TTGGTGTTTG ACAATATCGA ACTCTACGGA GAAGCTCACG AGATGAGAGA CGACCCCCCA 
ATCATTGTCA TTGAGATTTA TGATCAAGAC ACAGTAGGGA AGGCCGATTT CATGGGGAGA 
ACATTTGCCA AACCTGTTGT GAAAATGTCA GATGAGCGGT ACTGCCCTCC CCGCTTTCCG 
CCACAGCTGG AATACTACCA GATCTACCGT GGGAACTCAA CCGCAGGGGA CCTCTTGGGA 
GCTTTCGAAC TTCTCCAGAT CGGACCAGCG GGCAAGTCTG ACCTCCCACA GATTGAAGGA 
CCAACAGACA TAGAGAGAGG ACCCATCCTT CCAGTTCCTC TTGGAATCAG ACCAGTGCTC 
AGCAAATACA GAGTAGAGGT CCTTTTCTGG GGGCTGAGAG ACCTGAAGAG AGTAAATCTA 
GCCCAGGTTG ATAGACCAAG GGTGGATATT GAGTGTGCCG GGAGGGGGGT GCAGTCTGCC 
CTCATCCAGA ACTACAAGAA GAATCCAAAT TTTAGCACGT TGGTCAAATG GTTTGAAGTG 
GATCTCCCCG AGAATGAGTT ACTTCACCCG CCATTGAATA TCCGAGTAGT AGACTGCCGA 
GCCTTTGGCA GATACACTCT GGTTGGTTCC CATGCTGTCA GCTCCCTACG CAGGTTCATT 
TACCGGCCTC CGGATAAGAA CTCCCAGCAG TGGACCACAG CAGCTAAACT CATGAATGGC 
TACTTGGCAA TGACCAATGG AAGGCCCCGC TCTCGCACCA CAGGTGAAAT AGTTATCAAC 
ATGGAGCCCG AAGCGCCCGT CAAGAAGATG GAGACAATGG TGAAACTGGA AGCCAATTCC 
GACGCGGTGG TTAAAGTAGA CGTGAGCGAG GAAGAAAAAG ACAAGAAGAA GAAGAAGAAG 
AAAGGAGTAG AGGAGATAGA GGAAGAGGAG CCAGATGAGA GCATGTTGGA CTGGTGGTCC 
AAATACTTTG CTTCCATTGA AACCATGAAG GAGCAACTGC GCCAACAGGA AGCGGCAGCC 
GCCGAAGCCG AAGAGAAGGA AGAGATGGAG ATCGCAGAGG AGATCAAAAC TGATGACTCT 
CCCATGAAAG GCAACAAGGG CCCCACGAAG AACAAGGAGA AGGTCAAAGC CCCAAAAGAG 
GAGAAGAAGA ACAAGAAGCA GCAACAGCAG CAGCAACAAG CCGATCAGGC CGAGAAGAAG 
AACAAGCAAA AGATCGATGA GTTAAAGGTG TACAACAAGG AGTTGGAGAC CGAGTATGAC 
AACTTTGAGG ACTGGCTCCA TACATTTAAC TTGTTACGAG GGAAGATTGG AGATGATGAC 
GATACTGTAA GCGAAGAAGA GCGAATTGTT GGGAGGTTTA AGGGTTCCAT GTGTGTGTAC 
AAAGTTCCTT TGCCAGAAGA CGTCACAAAG GAAGCCGGGT ATGACCCAAA CTTCGGCATG 
TTCCAAGGAA TCCCTAACAA CGACCCAATG AATGTGCTGG TCAGAGTTTA TGTCGTGAGG 
GCCACCGATC TTCATCCAGC TGATATCAAT GGTAAAGCGG ATCCATATGT TGTCATAAAA 
CTAGGAAAAA CTGATATAAA AGATAAGGAA AACTACATCT CCAAGCAGCT AAACCCTGTC 
TTCGGAAAGT CCTTTGATAT TGAGGCCACT TTTCCGATGG AGTCCATGCT TACAGTTGCG 
GTTTATGACT GGGATCTGGT GGGGACGGAT GATCTGATCG GTGAAACCAA AATCGACCTG 
GAAAACCGTT ACTACAGCAA GCACAGGGCG ACTTGTGGTA TCTCAAAGAC ATATGCCATT 
CACGGGTACA ACTTGTGGAG GGACCCAGTG AAGCCTACAC AAATCTTATC CAAACTTTGC 
AAAGAAGGGA AGATCGATGG ACCTCATTAT GGCCCTGGTG GAAGAGTGAA AGTCACGAAT 
CGGGTATTCA CTGGTCCCAC AGAAATTGAT GATGAAAATG GTCAGAAGAA ACAAACGGAT 
GAGCACCTTG CACTCTCGGT CCTTCAGCAC TGGGAGGACA TTCCTCGGGT GGGATGTAAG 
CTGGTCCCAG AGCATGTAGA AACGAGGCCT TTGTTAAACC CAGACAAACC TGGAATTGAG 
CAGGGCCGCA TAGAAATGTG GGTAGACATG TTCCCCATGG ACATGCCTGC GCCAGGTCCT 
GCCATTGATA TCTCTCCAAG GAAGCCAAAA AGATACGAGC TTAGAGTGAT AATATGGAAT 
ACAGATGAGG TAATCCTGGA AGATGATGAT TACTTCACAG GAGAAAAATC TAGTGACATA 
TTTGTCAGGG GGTGGCTGAA GGGACAGCAG GAAGACAAAC AAGACACGGA TGTCCACTAT 
CATTCCCTTA CTGGGGAGGG GAACTTCAAC TGGAGGTTCT TGTTCCCTTT TGATTACCTT 
ATGGCAGAGG AGAAGATTGT CATCTCCAAG AAAGAGTCCA TGTTCTCTTG GGATGAGACC 
GAGTACAAGA TCCCGGCTCG TCTCACTCTC CAGGTCTGGG ATGCCGACCA CTTCTCAGCA 
GATGACTTTC TCGGAGCCAT AGAATTAGAC TTGAATCGCT TTCCCCGAGG GGCAAAAACA 
GCGAAACAGT GTTCGATAGA AATGGCGGAA CCCGACGTCG ATCTGCCAAT GGTCTCAATC 
TTCAAACAGA AGAGGGTGAA GGGATGGTGG CCTTTCGTTG CCAGGAATGA AAACGATGAG 
ATGGAGCTGA CTGGTAAAGT GGAGGCGGAG CTTCACCTTC TCTCAGCAGA GGAGGCAGAG 
AAGACCCCGG CAGGGCTTGG GCGCAATGAA CCAGATCCTC TTGAAAAACC AAATCGACCC 
GACACCTCGT TCATCTGGTT CCTCAACCCA CTTAAGTCCA TCAAGTATCT CATCTGTAAT 
CGCTACAAGT GGCTCATCAT TAAGATTGTG CTGGCGTTGC TGCTTTTGGC CATGGTGGCC 
CTCTTCCTCT ACAGCATGCC CGGTTACATG GTGAAGAAGC TTCTGGGTGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757741.1
CDS	97..4830
Translation	MNTSIMANVKKALIGENKDLVDPYVQVAFAGQKGKTSVQKSSYEPMWNEQIIFTEMFPPLCKRMKVQIRDSDKVNDVAIGTHFIDLRKIANEGDKGFLPTFGPAWVNMYGSTRNYTLMDEHQDLNEGLGEGVSFRARLLLSLAVEILDTTNPELTSSTEVQVEGAQPVAETCTGKMEEFFLFGAFLESTMIDRKIGDKPINFEVTIGNYGNQIDGISKPSMRKRKEGGDGVEEETELLQNSSDDEADEDGEMLSISSSPAMKPLITDRNYFHLPFFDKKPCTYIKSWWQDQRRRLYNANIMDKIADKLEEGLNDVHEMIKTEKPYPERRLRGVLEELSSSCFRFVTLANKDQNQNGQTKLDRERLKSCMRELENMGTQAKTMRSQVKKNTVKDKLKLVQNFLQKLRFLADEPQHSIPDIFIWMMSNNKRIAYARIPSKDILYSIVDEETGKDCGKVKCVFLKLPGKRGFGPAGWTVQAKMEVYMWLGLSKQRRDFLCGLPCGFEEIKAVKGPGLQSFPPISLSYTKKQVFQLRAHMYQARSLFAADSSGLSDPFARVFFNTQSQCTEVLNETLCPTWDQLLVFDNIELYGEAHEMRDDPPIIVIEIYDQDTVGKADFMGRTFAKPVVKMSDERYCPPRFPPQLEYYQIYRGNSTAGDLLGAFELLQIGPAGKSDLPQIEGPTDIERGPILPVPLGIRPVLSKYRVEVLFWGLRDLKRVNLAQVDRPRVDIECAGRGVQSALIQNYKKNPNFSTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHAVSSLRRFIYRPPDKNSQQWTTAAKLMNGYLAMTNGRPRSRTTGEIVINMEPEAPVKKMETMVKLEANSDAVVKVDVSEEEKDKKKKKKKGVEEIEEEEPDESMLDWWSKYFASIETMKEQLRQQEAAAAEAEEKEEMEIAEEIKTDDSPMKGNKGPTKNKEKVKAPKEEKKNKKQQQQQQQADQAEKKNKQKIDELKVYNKELETEYDNFEDWLHTFNLLRGKIGDDDDTVSEEERIVGRFKGSMCVYKVPLPEDVTKEAGYDPNFGMFQGIPNNDPMNVLVRVYVVRATDLHPADINGKADPYVVIKLGKTDIKDKENYISKQLNPVFGKSFDIEATFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRYYSKHRATCGISKTYAIHGYNLWRDPVKPTQILSKLCKEGKIDGPHYGPGGRVKVTNRVFTGPTEIDDENGQKKQTDEHLALSVLQHWEDIPRVGCKLVPEHVETRPLLNPDKPGIEQGRIEMWVDMFPMDMPAPGPAIDISPRKPKRYELRVIIWNTDEVILEDDDYFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRFLFPFDYLMAEEKIVISKKESMFSWDETEYKIPARLTLQVWDADHFSADDFLGAIELDLNRFPRGAKTAKQCSIEMAEPDVDLPMVSIFKQKRVKGWWPFVARNENDEMELTGKVEAELHLLSAEEAEKTPAGLGRNEPDPLEKPNRPDTSFIWFLNPLKSIKYLICNRYKWLIIKIVLALLLLAMVALFLYSMPGYMVKKLLGA

Target ORF information:

RefSeq Version	XM_031901881.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis otoferlin (otof), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901881.1

ORF Insert Sequence:

ATGAACACCA GCATCATGGC CAACGTCAAG AAAGCTCTCA TTGGAGAAAA CAAGGACTTG 
GTGGACCCCT ATGTGCAAGT TGCCTTTGCA GGGCAGAAGG GAAAGACCTC GGTGCAGAAG 
AGCAGCTACG AGCCCATGTG GAACGAGCAG ATCATTTTCA CCGAGATGTT CCCCCCCCTG 
TGCAAGAGGA TGAAAGTGCA GATCAGAGAT TCCGACAAGG TCAACGATGT CGCCATTGGG 
ACCCACTTCA TTGACCTGAG GAAAATTGCC AACGAGGGGG ACAAGGGATT CCTCCCTACT 
TTCGGCCCAG CCTGGGTGAA TATGTATGGC TCCACCCGTA ACTACACCCT CATGGATGAG 
CACCAGGATC TGAATGAGGG GCTCGGAGAA GGCGTCTCTT TCCGCGCAAG GCTGCTGCTG 
AGTCTCGCTG TTGAGATACT GGATACAACC AACCCAGAGC TCACCAGTTC CACAGAGGTT 
CAAGTTGAAG GAGCTCAACC AGTGGCAGAA ACCTGTACCG GTAAAATGGA AGAGTTCTTC 
CTGTTTGGAG CATTCCTGGA ATCCACCATG ATTGATCGGA AAATTGGGGA CAAGCCAATT 
AACTTTGAGG TCACCATAGG TAACTATGGC AACCAAATAG ATGGAATTTC CAAGCCGAGT 
ATGAGGAAGA GGAAGGAAGG AGGTGACGGA GTCGAGGAGG AGACGGAGCT CTTGCAGAAT 
TCCAGTGATG ATGAGGCTGA TGAAGATGGA GAAATGTTAT CAATCTCTTC GTCCCCTGCA 
ATGAAGCCAC TCATAACTGA TAGGAACTAC TTCCACCTCC CGTTCTTTGA TAAGAAGCCC 
TGTACCTACA TTAAGAGTTG GTGGCAGGAC CAGCGGAGGC GGCTGTACAA TGCCAACATT 
ATGGACAAGA TCGCAGATAA GCTGGAGGAA GGCTTGAACG ATGTTCATGA GATGATAAAG 
ACGGAGAAGC CCTACCCTGA ACGGCGGCTG CGAGGAGTCC TGGAGGAGCT GAGTAGCAGC 
TGTTTTCGAT TTGTTACCTT GGCCAACAAG GACCAGAACC AGAATGGGCA GACGAAACTG 
GACAGGGAGA GGCTGAAGTC CTGCATGAGG GAGCTGGAGA ACATGGGGAC ACAAGCCAAG 
ACCATGAGGT CCCAAGTGAA GAAGAACACG GTTAAGGACA AGCTGAAACT GGTGCAGAAT 
TTCCTGCAGA AGCTCCGGTT CCTGGCAGAT GAGCCCCAGC ACAGCATTCC TGACATCTTC 
ATCTGGATGA TGTCCAACAA CAAGCGCATC GCCTACGCTC GAATCCCTTC CAAAGATATC 
CTGTACTCCA TTGTGGATGA GGAGACGGGA AAGGACTGTG GCAAAGTCAA GTGTGTCTTC 
CTTAAGCTGC CGGGCAAGAG GGGCTTTGGG CCTGCAGGCT GGACGGTACA AGCCAAGATG 
GAGGTCTACA TGTGGTTGGG CCTTAGCAAG CAGCGCAGGG ATTTCTTGTG TGGTCTTCCA 
TGTGGATTTG AGGAGATAAA AGCAGTGAAA GGGCCCGGTC TGCAGTCCTT CCCACCCATC 
AGCCTCAGCT ACACCAAAAA ACAAGTGTTC CAGTTACGAG CCCACATGTA CCAAGCCCGG 
AGTCTCTTTG CCGCTGACAG CAGTGGACTT TCCGACCCCT TTGCCCGCGT CTTCTTCAAC 
ACTCAGAGCC AATGTACAGA GGTGCTGAAT GAGACCCTTT GTCCGACTTG GGACCAGCTG 
TTGGTGTTTG ACAATATCGA ACTCTACGGA GAAGCTCACG AGATGAGAGA CGACCCCCCA 
ATCATTGTCA TTGAGATTTA TGATCAAGAC ACAGTAGGGA AGGCCGATTT CATGGGGAGA 
ACATTTGCCA AACCTGTTGT GAAAATGTCA GATGAGCGGT ACTGCCCTCC CCGCTTTCCG 
CCACAGCTGG AATACTACCA GATCTACCGT GGGAACTCAA CCGCAGGGGA CCTCTTGGGA 
GCTTTCGAAC TTCTCCAGAT CGGACCAGCG GGCAAGTCTG ACCTCCCACA GATTGAAGGA 
CCAACAGACA TAGAGAGAGG ACCCATCCTT CCAGTTCCTC TTGGAATCAG ACCAGTGCTC 
AGCAAATACA GAGTAGAGGT CCTTTTCTGG GGGCTGAGAG ACCTGAAGAG AGTAAATCTA 
GCCCAGGTTG ATAGACCAAG GGTGGATATT GAGTGTGCCG GGAGGGGGGT GCAGTCTGCC 
CTCATCCAGA ACTACAAGAA GAATCCAAAT TTTAGCACGT TGGTCAAATG GTTTGAAGTG 
GATCTCCCCG AGAATGAGTT ACTTCACCCG CCATTGAATA TCCGAGTAGT AGACTGCCGA 
GCCTTTGGCA GATACACTCT GGTTGGTTCC CATGCTGTCA GCTCCCTACG CAGGTTCATT 
TACCGGCCTC CGGATAAGAA CTCCCAGCAG TGGACCACAG CAGCTAAACT CATGAATGGC 
TACTTGGCAA TGACCAATGG AAGGCCCCGC TCTCGCACCA CAGGTGAAAT AGTTATCAAC 
ATGGAGCCCG AAGCGCCCGT CAAGAAGATG GAGACAATGG TGAAACTGGA AGCCAATTCC 
GACGCGGTGG TTAAAGTAGA CGTGAGCGAG GAAGAAAAAG ACAAGAAGAA GAAGAAGAAG 
AAAGGAGTAG AGGAGATAGA GGAAGAGGAG CCAGATGAGA GCATGTTGGA CTGGTGGTCC 
AAATACTTTG CTTCCATTGA AACCATGAAG GAGCAACTGC GCCAACAGGA AGCGGCAGCC 
GCCGAAGCCG AAGAGAAGGA AGAGATGGAG ATCGCAGAGG AGATCAAAAC TGATGACTCT 
CCCATGAAAG GCAACAAGGG CCCCACGAAG AACAAGGAGA AGGTCAAAGC CCCAAAAGAG 
GAGAAGAAGA ACAAGAAGCA GCAACAGCAG CAGCAACAAG CCGATCAGGC CGAGAAGAAG 
AACAAGCAAA AGATCGATGA GTTAAAGGTG TACAACAAGG AGTTGGAGAC CGAGTATGAC 
AACTTTGAGG ACTGGCTCCA TACATTTAAC TTGTTACGAG GGAAGATTGG AGATGATGAC 
GATACTGTAA GCGAAGAAGA GCGAATTGTT GGGAGGTTTA AGGGTTCCAT GTGTGTGTAC 
AAAGTTCCTT TGCCAGAAGA CGTCACAAAG GAAGCCGGGT ATGACCCAAA CTTCGGCATG 
TTCCAAGGAA TCCCTAACAA CGACCCAATG AATGTGCTGG TCAGAGTTTA TGTCGTGAGG 
GCCACCGATC TTCATCCAGC TGATATCAAT GGTAAAGCGG ATCCATATGT TGTCATAAAA 
CTAGGAAAAA CTGATATAAA AGATAAGGAA AACTACATCT CCAAGCAGCT AAACCCTGTC 
TTCGGAAAGT CCTTTGATAT TGAGGCCACT TTTCCGATGG AGTCCATGCT TACAGTTGCG 
GTTTATGACT GGGATCTGGT GGGGACGGAT GATCTGATCG GTGAAACCAA AATCGACCTG 
GAAAACCGTT ACTACAGCAA GCACAGGGCG ACTTGTGGTA TCTCAAAGAC ATATGCCATT 
CACGGGTACA ACTTGTGGAG GGACCCAGTG AAGCCTACAC AAATCTTATC CAAACTTTGC 
AAAGAAGGGA AGATCGATGG ACCTCATTAT GGCCCTGGTG GAAGAGTGAA AGTCACGAAT 
CGGGTATTCA CTGGTCCCAC AGAAATTGAT GATGAAAATG GTCAGAAGAA ACAAACGGAT 
GAGCACCTTG CACTCTCGGT CCTTCAGCAC TGGGAGGACA TTCCTCGGGT GGGATGTAAG 
CTGGTCCCAG AGCATGTAGA AACGAGGCCT TTGTTAAACC CAGACAAACC TGGAATTGAG 
CAGGGCCGCA TAGAAATGTG GGTAGACATG TTCCCCATGG ACATGCCTGC GCCAGGTCCT 
GCCATTGATA TCTCTCCAAG GAAGCCAAAA AGATACGAGC TTAGAGTGAT AATATGGAAT 
ACAGATGAGG TAATCCTGGA AGATGATGAT TACTTCACAG GAGAAAAATC TAGTGACATA 
TTTGTCAGGG GGTGGCTGAA GGGACAGCAG GAAGACAAAC AAGACACGGA TGTCCACTAT 
CATTCCCTTA CTGGGGAGGG GAACTTCAAC TGGAGGTTCT TGTTCCCTTT TGATTACCTT 
ATGGCAGAGG AGAAGATTGT CATCTCCAAG AAAGAGTCCA TGTTCTCTTG GGATGAGACC 
GAGTACAAGA TCCCGGCTCG TCTCACTCTC CAGGTCTGGG ATGCCGACCA CTTCTCAGCA 
GATGACTTTC TCGGAGCCAT AGAATTAGAC TTGAATCGCT TTCCCCGAGG GGCAAAAACA 
GCGAAACAGT GTTCGATAGA AATGGCGGAA CCCGACGTCG ATCTGCCAAT GGTCTCAATC 
TTCAAACAGA AGAGGGTGAA GGGATGGTGG CCTTTCGTTG CCAGGAATGA AAACGATGAG 
ATGGAGCTGA CTGGTAAAGT GGAGGCGGAG CTTCACCTTC TCTCAGCAGA GGAGGCAGAG 
AAGACCCCGG CAGGGCTTGG GCGCAATGAA CCAGATCCTC TTGAAAAACC AAATCGACCC 
GACACCTCGT TCATCTGGTT CCTCAACCCA CTTAAGTCCA TCAAGTATCT CATCTGTAAT 
CGCTACAAGT GGCTCATCAT TAAGATTGTG CTGGCGTTGC TGCTTTTGGC CATGGTGGCC 
CTCTTCCTCT ACAGCATGCC CGGTTACATG GTGAAGAAGC TTCTGGGTGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

otof ( XM_031901882.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757742.1 Xenopus tropicalis otoferlin (otof), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa58170
Clone ID Related Accession (Same CDS sequence)	XM_031901882.1
Accession Version	XM_031901882.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4734bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	otoferlin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACACCA GCATCATGGC CAACGTCAAG AAAGCTCTCA TTGGAGAAAA CAAGGACTTG 
GTGGACCCCT ATGTGCAAGT TGCCTTTGCA GGGCAGAAGG GAAAGACCTC GGTGCAGAAG 
AGCAGCTACG AGCCCATGTG GAACGAGCAG ATCATTTTCA CCGAGATGTT CCCCCCCCTG 
TGCAAGAGGA TGAAAGTGCA GATCAGAGAT TCCGACAAGG TCAACGATGT CGCCATTGGG 
ACCCACTTCA TTGACCTGAG GAAAATTGCC AACGAGGGGG ACAAGGGATT CCTCCCTACT 
TTCGGCCCAG CCTGGGTGAA TATGTATGGC TCCACCCGTA ACTACACCCT CATGGATGAG 
CACCAGGATC TGAATGAGGG GCTCGGAGAA GGCGTCTCTT TCCGCGCAAG GCTGCTGCTG 
AGTCTCGCTG TTGAGATACT GGATACAACC AACCCAGAGC TCACCAGTTC CACAGAGGTT 
CAAGTTGAAG GAGCTCAACC AGTGGCAGAA ACCTGTACCG GTAAAATGGA AGAGTTCTTC 
CTGTTTGGAG CATTCCTGGA ATCCACCATG ATTGATCGGA AAATTGGGGA CAAGCCAATT 
AACTTTGAGG TCACCATAGG TAACTATGGC AACCAAATAG ATGGAATTTC CAAGCCGAGT 
ATGAGGAAGA GGAAGGAAGG AGGTGACGGA GTCGAGGAGG AGACGGAGCT CTTGCAGAAT 
TCCAGTGATG ATGAGGCTGA TGAAGATGGA GAAATGTTAT CAATCTCTTC GTCCCCTGCA 
ATGAAGCCAC TCATAACTGA TAGGAACTAC TTCCACCTCC CGTTCTTTGA TAAGAAGCCC 
TGTACCTACA TTAAGAGTTG GTGGCAGGAC CAGCGGAGGC GGCTGTACAA TGCCAACATT 
ATGGACAAGA TCGCAGATAA GCTGGAGGAA GGCTTGAACG ATGTTCATGA GATGATAAAG 
ACGGAGAAGC CCTACCCTGA ACGGCGGCTG CGAGGAGTCC TGGAGGAGCT GAGTAGCAGC 
TGTTTTCGAT TTGTTACCTT GGCCAACAAG GACCAGAACC AGAATGGGCA GACGAAACTG 
GACAGGGAGA GGCTGAAGTC CTGCATGAGG GAGCTGGAGA ACATGGGGAC ACAAGCCAAG 
ACCATGAGGT CCCAAGTGAA GAAGAACACG GTTAAGGACA AGCTGAAACT GGTGCAGAAT 
TTCCTGCAGA AGCTCCGGTT CCTGGCAGAT GAGCCCCAGC ACAGCATTCC TGACATCTTC 
ATCTGGATGA TGTCCAACAA CAAGCGCATC GCCTACGCTC GAATCCCTTC CAAAGATATC 
CTGTACTCCA TTGTGGATGA GGAGACGGGA AAGGACTGTG GCAAAGTCAA GTGTGTCTTC 
CTTAAGCTGC CGGGCAAGAG GGGCTTTGGG CCTGCAGGCT GGACGGTACA AGCCAAGATG 
GAGGTCTACA TGTGGTTGGG CCTTAGCAAG CAGCGCAGGG ATTTCTTGTG TGGTCTTCCA 
TGTGGATTTG AGGAGATAAA AGCAGTGAAA GGGCCCGGTC TGCAGTCCTT CCCACCCATC 
AGCCTCAGCT ACACCAAAAA ACAAGTGTTC CAGTTACGAG CCCACATGTA CCAAGCCCGG 
AGTCTCTTTG CCGCTGACAG CAGTGGACTT TCCGACCCCT TTGCCCGCGT CTTCTTCAAC 
ACTCAGAGCC AATGTACAGA GGTGCTGAAT GAGACCCTTT GTCCGACTTG GGACCAGCTG 
TTGGTGTTTG ACAATATCGA ACTCTACGGA GAAGCTCACG AGATGAGAGA CGACCCCCCA 
ATCATTGTCA TTGAGATTTA TGATCAAGAC ACAGTAGGGA AGGCCGATTT CATGGGGAGA 
ACATTTGCCA AACCTGTTGT GAAAATGTCA GATGAGCGGT ACTGCCCTCC CCGCTTTCCG 
CCACAGCTGG AATACTACCA GATCTACCGT GGGAACTCAA CCGCAGGGGA CCTCTTGGGA 
GCTTTCGAAC TTCTCCAGAT CGGACCAGCG GGCAAGTCTG ACCTCCCACA GATTGAAGGA 
CCAACAGACA TAGAGAGAGG ACCCATCCTT CCAGTTCCTC TTGGAATCAG ACCAGTGCTC 
AGCAAATACA GAGTAGAGGT CCTTTTCTGG GGGCTGAGAG ACCTGAAGAG AGTAAATCTA 
GCCCAGGTTG ATAGACCAAG GGTGGATATT GAGTGTGCCG GGAGGGGGGT GCAGTCTGCC 
CTCATCCAGA ACTACAAGAA GAATCCAAAT TTTAGCACGT TGGTCAAATG GTTTGAAGTG 
GATCTCCCCG AGAATGAGTT ACTTCACCCG CCATTGAATA TCCGAGTAGT AGACTGCCGA 
GCCTTTGGCA GATACACTCT GGTTGGTTCC CATGCTGTCA GCTCCCTACG CAGGTTCATT 
TACCGGCCTC CGGATAAGAA CTCCCAGCAG TGGACCACAG CAGCTAAACT CATGAATGGC 
TACTTGGCAA TGACCAATGG AAGGCCCCGC TCTCGCACCA CAGGTGAAAT AGTTATCAAC 
ATGGAGCCCG AAGCGCCCGT CAAGAAGATG GAGACAATGG TGAAACTGGA AGCCAATTCC 
GACGCGGTGG TTAAAGTAGA CGTGAGCGAG GAAGAAAAAG ACAAGAAGAA GAAGAAGAAG 
AAAGGAGTAG AGGAGATAGA GGAAGAGGAG CCAGATGAGA GCATGTTGGA CTGGTGGTCC 
AAATACTTTG CTTCCATTGA AACCATGAAG GAGCAACTGC GCCAACAGGA AGCGGCAGCC 
GCCGAAGCCG AAGAGAAGGA AGAGATGGAG ATCGCAGAGG AGATCAAAAC TGATGACTCT 
CCCATGAAAG GCAACAAGGG CCCCACGAAG AACAAGGAGA AGGTCAAAGC CCCAAAAGAG 
GAGAAGAAGA ACAAGAAGCA GCAACAGCAG CAGCAACAAG CCGATCAGGC CGAGAAGAAG 
AACAAGCAAA AGATCGATGA GTTAAAGGTG TACAACAAGG AGTTGGAGAC CGAGTATGAC 
AACTTTGAGG ACTGGCTCCA TACATTTAAC TTGTTACGAG GGAAGATTGG AGATGATGAC 
GATACTGTAA GCGAAGAAGA GCGAATTGTT GGGAGGTTTA AGGGTTCCAT GTGTGTGTAC 
AAAGTTCCTT TGCCAGAAGA CGTCACAAAG GAAGCCGGGT ATGACCCAAA CTTCGGCATG 
TTCCAAGGAA TCCCTAACAA CGACCCAATG AATGTGCTGG TCAGAGTTTA TGTCGTGAGG 
GCCACCGATC TTCATCCAGC TGATATCAAT GGTAAAGCGG ATCCATATGT TGTCATAAAA 
CTAGGAAAAA CTGATATAAA AGATAAGGAA AACTACATCT CCAAGCAGCT AAACCCTGTC 
TTCGGAAAGT CCTTTGATAT TGAGGCCACT TTTCCGATGG AGTCCATGCT TACAGTTGCG 
GTTTATGACT GGGATCTGGT GGGGACGGAT GATCTGATCG GTGAAACCAA AATCGACCTG 
GAAAACCGTT ACTACAGCAA GCACAGGGCG ACTTGTGGTA TCTCAAAGAC ATATGCCATT 
CACGGGTACA ACTTGTGGAG GGACCCAGTG AAGCCTACAC AAATCTTATC CAAACTTTGC 
AAAGAAGGGA AGATCGATGG ACCTCATTAT GGCCCTGGTG GAAGAGTGAA AGTCACGAAT 
CGGGTATTCA CTGGTCCCAC AGAAATTGAT GATGAAAATG GTCAGAAGAA ACAAACGGAT 
GAGCACCTTG CACTCTCGGT CCTTCAGCAC TGGGAGGACA TTCCTCGGGT GGGATGTAAG 
CTGGTCCCAG AGCATGTAGA AACGAGGCCT TTGTTAAACC CAGACAAACC TGGAATTGAG 
CAGGGCCGCA TAGAAATGTG GGTAGACATG TTCCCCATGG ACATGCCTGC GCCAGGTCCT 
GCCATTGATA TCTCTCCAAG GAAGCCAAAA AGTTTTGAGC TACGTGTTAT TATTTGGAAC 
ACTGATGAAG TGATTCTAGA AGATGATGCT TTCATGACAG GAGAGAAGAT GTCTGACATC 
TATGTCAAGG GGTGGCTGAA GGGACAGCAG GAAGACAAAC AAGACACGGA TGTCCACTAT 
CATTCCCTTA CTGGGGAGGG GAACTTCAAC TGGAGGTTCT TGTTCCCTTT TGATTACCTT 
ATGGCAGAGG AGAAGATTGT CATCTCCAAG AAAGAGTCCA TGTTCTCTTG GGATGAGACC 
GAGTACAAGA TCCCGGCTCG TCTCACTCTC CAGGTCTGGG ATGCCGACCA CTTCTCAGCA 
GATGACTTTC TCGGAGCCAT AGAATTAGAC TTGAATCGCT TTCCCCGAGG GGCAAAAACA 
GCGAAACAGT GTTCGATAGA AATGGCGGAA CCCGACGTCG ATCTGCCAAT GGTCTCAATC 
TTCAAACAGA AGAGGGTGAA GGGATGGTGG CCTTTCGTTG CCAGGAATGA AAACGATGAG 
ATGGAGCTGA CTGGTAAAGT GGAGGCGGAG CTTCACCTTC TCTCAGCAGA GGAGGCAGAG 
AAGACCCCGG CAGGGCTTGG GCGCAATGAA CCAGATCCTC TTGAAAAACC AAACCGCCCC 
GATACAAGTT TCATCTGGTT TCTGAACCCC CTGAAGTCTA TCCGCTATTT CATCTGGCAC 
ACTTATCGCT GGTTGATCCT GAAAATCCTC ATCCTCATCC TCCTTCTCCT CATGGTGGGG 
CTTTTCCTTT ACTCTTTGCC TGGATACCTG GTAAAGAAGA TCCTGGGAGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757742.1
CDS	97..4830
Translation	MNTSIMANVKKALIGENKDLVDPYVQVAFAGQKGKTSVQKSSYEPMWNEQIIFTEMFPPLCKRMKVQIRDSDKVNDVAIGTHFIDLRKIANEGDKGFLPTFGPAWVNMYGSTRNYTLMDEHQDLNEGLGEGVSFRARLLLSLAVEILDTTNPELTSSTEVQVEGAQPVAETCTGKMEEFFLFGAFLESTMIDRKIGDKPINFEVTIGNYGNQIDGISKPSMRKRKEGGDGVEEETELLQNSSDDEADEDGEMLSISSSPAMKPLITDRNYFHLPFFDKKPCTYIKSWWQDQRRRLYNANIMDKIADKLEEGLNDVHEMIKTEKPYPERRLRGVLEELSSSCFRFVTLANKDQNQNGQTKLDRERLKSCMRELENMGTQAKTMRSQVKKNTVKDKLKLVQNFLQKLRFLADEPQHSIPDIFIWMMSNNKRIAYARIPSKDILYSIVDEETGKDCGKVKCVFLKLPGKRGFGPAGWTVQAKMEVYMWLGLSKQRRDFLCGLPCGFEEIKAVKGPGLQSFPPISLSYTKKQVFQLRAHMYQARSLFAADSSGLSDPFARVFFNTQSQCTEVLNETLCPTWDQLLVFDNIELYGEAHEMRDDPPIIVIEIYDQDTVGKADFMGRTFAKPVVKMSDERYCPPRFPPQLEYYQIYRGNSTAGDLLGAFELLQIGPAGKSDLPQIEGPTDIERGPILPVPLGIRPVLSKYRVEVLFWGLRDLKRVNLAQVDRPRVDIECAGRGVQSALIQNYKKNPNFSTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHAVSSLRRFIYRPPDKNSQQWTTAAKLMNGYLAMTNGRPRSRTTGEIVINMEPEAPVKKMETMVKLEANSDAVVKVDVSEEEKDKKKKKKKGVEEIEEEEPDESMLDWWSKYFASIETMKEQLRQQEAAAAEAEEKEEMEIAEEIKTDDSPMKGNKGPTKNKEKVKAPKEEKKNKKQQQQQQQADQAEKKNKQKIDELKVYNKELETEYDNFEDWLHTFNLLRGKIGDDDDTVSEEERIVGRFKGSMCVYKVPLPEDVTKEAGYDPNFGMFQGIPNNDPMNVLVRVYVVRATDLHPADINGKADPYVVIKLGKTDIKDKENYISKQLNPVFGKSFDIEATFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRYYSKHRATCGISKTYAIHGYNLWRDPVKPTQILSKLCKEGKIDGPHYGPGGRVKVTNRVFTGPTEIDDENGQKKQTDEHLALSVLQHWEDIPRVGCKLVPEHVETRPLLNPDKPGIEQGRIEMWVDMFPMDMPAPGPAIDISPRKPKSFELRVIIWNTDEVILEDDAFMTGEKMSDIYVKGWLKGQQEDKQDTDVHYHSLTGEGNFNWRFLFPFDYLMAEEKIVISKKESMFSWDETEYKIPARLTLQVWDADHFSADDFLGAIELDLNRFPRGAKTAKQCSIEMAEPDVDLPMVSIFKQKRVKGWWPFVARNENDEMELTGKVEAELHLLSAEEAEKTPAGLGRNEPDPLEKPNRPDTSFIWFLNPLKSIRYFIWHTYRWLILKILILILLLLMVGLFLYSLPGYLVKKILGA

Target ORF information:

RefSeq Version	XM_031901882.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis otoferlin (otof), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901882.1

ORF Insert Sequence:

ATGAACACCA GCATCATGGC CAACGTCAAG AAAGCTCTCA TTGGAGAAAA CAAGGACTTG 
GTGGACCCCT ATGTGCAAGT TGCCTTTGCA GGGCAGAAGG GAAAGACCTC GGTGCAGAAG 
AGCAGCTACG AGCCCATGTG GAACGAGCAG ATCATTTTCA CCGAGATGTT CCCCCCCCTG 
TGCAAGAGGA TGAAAGTGCA GATCAGAGAT TCCGACAAGG TCAACGATGT CGCCATTGGG 
ACCCACTTCA TTGACCTGAG GAAAATTGCC AACGAGGGGG ACAAGGGATT CCTCCCTACT 
TTCGGCCCAG CCTGGGTGAA TATGTATGGC TCCACCCGTA ACTACACCCT CATGGATGAG 
CACCAGGATC TGAATGAGGG GCTCGGAGAA GGCGTCTCTT TCCGCGCAAG GCTGCTGCTG 
AGTCTCGCTG TTGAGATACT GGATACAACC AACCCAGAGC TCACCAGTTC CACAGAGGTT 
CAAGTTGAAG GAGCTCAACC AGTGGCAGAA ACCTGTACCG GTAAAATGGA AGAGTTCTTC 
CTGTTTGGAG CATTCCTGGA ATCCACCATG ATTGATCGGA AAATTGGGGA CAAGCCAATT 
AACTTTGAGG TCACCATAGG TAACTATGGC AACCAAATAG ATGGAATTTC CAAGCCGAGT 
ATGAGGAAGA GGAAGGAAGG AGGTGACGGA GTCGAGGAGG AGACGGAGCT CTTGCAGAAT 
TCCAGTGATG ATGAGGCTGA TGAAGATGGA GAAATGTTAT CAATCTCTTC GTCCCCTGCA 
ATGAAGCCAC TCATAACTGA TAGGAACTAC TTCCACCTCC CGTTCTTTGA TAAGAAGCCC 
TGTACCTACA TTAAGAGTTG GTGGCAGGAC CAGCGGAGGC GGCTGTACAA TGCCAACATT 
ATGGACAAGA TCGCAGATAA GCTGGAGGAA GGCTTGAACG ATGTTCATGA GATGATAAAG 
ACGGAGAAGC CCTACCCTGA ACGGCGGCTG CGAGGAGTCC TGGAGGAGCT GAGTAGCAGC 
TGTTTTCGAT TTGTTACCTT GGCCAACAAG GACCAGAACC AGAATGGGCA GACGAAACTG 
GACAGGGAGA GGCTGAAGTC CTGCATGAGG GAGCTGGAGA ACATGGGGAC ACAAGCCAAG 
ACCATGAGGT CCCAAGTGAA GAAGAACACG GTTAAGGACA AGCTGAAACT GGTGCAGAAT 
TTCCTGCAGA AGCTCCGGTT CCTGGCAGAT GAGCCCCAGC ACAGCATTCC TGACATCTTC 
ATCTGGATGA TGTCCAACAA CAAGCGCATC GCCTACGCTC GAATCCCTTC CAAAGATATC 
CTGTACTCCA TTGTGGATGA GGAGACGGGA AAGGACTGTG GCAAAGTCAA GTGTGTCTTC 
CTTAAGCTGC CGGGCAAGAG GGGCTTTGGG CCTGCAGGCT GGACGGTACA AGCCAAGATG 
GAGGTCTACA TGTGGTTGGG CCTTAGCAAG CAGCGCAGGG ATTTCTTGTG TGGTCTTCCA 
TGTGGATTTG AGGAGATAAA AGCAGTGAAA GGGCCCGGTC TGCAGTCCTT CCCACCCATC 
AGCCTCAGCT ACACCAAAAA ACAAGTGTTC CAGTTACGAG CCCACATGTA CCAAGCCCGG 
AGTCTCTTTG CCGCTGACAG CAGTGGACTT TCCGACCCCT TTGCCCGCGT CTTCTTCAAC 
ACTCAGAGCC AATGTACAGA GGTGCTGAAT GAGACCCTTT GTCCGACTTG GGACCAGCTG 
TTGGTGTTTG ACAATATCGA ACTCTACGGA GAAGCTCACG AGATGAGAGA CGACCCCCCA 
ATCATTGTCA TTGAGATTTA TGATCAAGAC ACAGTAGGGA AGGCCGATTT CATGGGGAGA 
ACATTTGCCA AACCTGTTGT GAAAATGTCA GATGAGCGGT ACTGCCCTCC CCGCTTTCCG 
CCACAGCTGG AATACTACCA GATCTACCGT GGGAACTCAA CCGCAGGGGA CCTCTTGGGA 
GCTTTCGAAC TTCTCCAGAT CGGACCAGCG GGCAAGTCTG ACCTCCCACA GATTGAAGGA 
CCAACAGACA TAGAGAGAGG ACCCATCCTT CCAGTTCCTC TTGGAATCAG ACCAGTGCTC 
AGCAAATACA GAGTAGAGGT CCTTTTCTGG GGGCTGAGAG ACCTGAAGAG AGTAAATCTA 
GCCCAGGTTG ATAGACCAAG GGTGGATATT GAGTGTGCCG GGAGGGGGGT GCAGTCTGCC 
CTCATCCAGA ACTACAAGAA GAATCCAAAT TTTAGCACGT TGGTCAAATG GTTTGAAGTG 
GATCTCCCCG AGAATGAGTT ACTTCACCCG CCATTGAATA TCCGAGTAGT AGACTGCCGA 
GCCTTTGGCA GATACACTCT GGTTGGTTCC CATGCTGTCA GCTCCCTACG CAGGTTCATT 
TACCGGCCTC CGGATAAGAA CTCCCAGCAG TGGACCACAG CAGCTAAACT CATGAATGGC 
TACTTGGCAA TGACCAATGG AAGGCCCCGC TCTCGCACCA CAGGTGAAAT AGTTATCAAC 
ATGGAGCCCG AAGCGCCCGT CAAGAAGATG GAGACAATGG TGAAACTGGA AGCCAATTCC 
GACGCGGTGG TTAAAGTAGA CGTGAGCGAG GAAGAAAAAG ACAAGAAGAA GAAGAAGAAG 
AAAGGAGTAG AGGAGATAGA GGAAGAGGAG CCAGATGAGA GCATGTTGGA CTGGTGGTCC 
AAATACTTTG CTTCCATTGA AACCATGAAG GAGCAACTGC GCCAACAGGA AGCGGCAGCC 
GCCGAAGCCG AAGAGAAGGA AGAGATGGAG ATCGCAGAGG AGATCAAAAC TGATGACTCT 
CCCATGAAAG GCAACAAGGG CCCCACGAAG AACAAGGAGA AGGTCAAAGC CCCAAAAGAG 
GAGAAGAAGA ACAAGAAGCA GCAACAGCAG CAGCAACAAG CCGATCAGGC CGAGAAGAAG 
AACAAGCAAA AGATCGATGA GTTAAAGGTG TACAACAAGG AGTTGGAGAC CGAGTATGAC 
AACTTTGAGG ACTGGCTCCA TACATTTAAC TTGTTACGAG GGAAGATTGG AGATGATGAC 
GATACTGTAA GCGAAGAAGA GCGAATTGTT GGGAGGTTTA AGGGTTCCAT GTGTGTGTAC 
AAAGTTCCTT TGCCAGAAGA CGTCACAAAG GAAGCCGGGT ATGACCCAAA CTTCGGCATG 
TTCCAAGGAA TCCCTAACAA CGACCCAATG AATGTGCTGG TCAGAGTTTA TGTCGTGAGG 
GCCACCGATC TTCATCCAGC TGATATCAAT GGTAAAGCGG ATCCATATGT TGTCATAAAA 
CTAGGAAAAA CTGATATAAA AGATAAGGAA AACTACATCT CCAAGCAGCT AAACCCTGTC 
TTCGGAAAGT CCTTTGATAT TGAGGCCACT TTTCCGATGG AGTCCATGCT TACAGTTGCG 
GTTTATGACT GGGATCTGGT GGGGACGGAT GATCTGATCG GTGAAACCAA AATCGACCTG 
GAAAACCGTT ACTACAGCAA GCACAGGGCG ACTTGTGGTA TCTCAAAGAC ATATGCCATT 
CACGGGTACA ACTTGTGGAG GGACCCAGTG AAGCCTACAC AAATCTTATC CAAACTTTGC 
AAAGAAGGGA AGATCGATGG ACCTCATTAT GGCCCTGGTG GAAGAGTGAA AGTCACGAAT 
CGGGTATTCA CTGGTCCCAC AGAAATTGAT GATGAAAATG GTCAGAAGAA ACAAACGGAT 
GAGCACCTTG CACTCTCGGT CCTTCAGCAC TGGGAGGACA TTCCTCGGGT GGGATGTAAG 
CTGGTCCCAG AGCATGTAGA AACGAGGCCT TTGTTAAACC CAGACAAACC TGGAATTGAG 
CAGGGCCGCA TAGAAATGTG GGTAGACATG TTCCCCATGG ACATGCCTGC GCCAGGTCCT 
GCCATTGATA TCTCTCCAAG GAAGCCAAAA AGTTTTGAGC TACGTGTTAT TATTTGGAAC 
ACTGATGAAG TGATTCTAGA AGATGATGCT TTCATGACAG GAGAGAAGAT GTCTGACATC 
TATGTCAAGG GGTGGCTGAA GGGACAGCAG GAAGACAAAC AAGACACGGA TGTCCACTAT 
CATTCCCTTA CTGGGGAGGG GAACTTCAAC TGGAGGTTCT TGTTCCCTTT TGATTACCTT 
ATGGCAGAGG AGAAGATTGT CATCTCCAAG AAAGAGTCCA TGTTCTCTTG GGATGAGACC 
GAGTACAAGA TCCCGGCTCG TCTCACTCTC CAGGTCTGGG ATGCCGACCA CTTCTCAGCA 
GATGACTTTC TCGGAGCCAT AGAATTAGAC TTGAATCGCT TTCCCCGAGG GGCAAAAACA 
GCGAAACAGT GTTCGATAGA AATGGCGGAA CCCGACGTCG ATCTGCCAAT GGTCTCAATC 
TTCAAACAGA AGAGGGTGAA GGGATGGTGG CCTTTCGTTG CCAGGAATGA AAACGATGAG 
ATGGAGCTGA CTGGTAAAGT GGAGGCGGAG CTTCACCTTC TCTCAGCAGA GGAGGCAGAG 
AAGACCCCGG CAGGGCTTGG GCGCAATGAA CCAGATCCTC TTGAAAAACC AAACCGCCCC 
GATACAAGTT TCATCTGGTT TCTGAACCCC CTGAAGTCTA TCCGCTATTT CATCTGGCAC 
ACTTATCGCT GGTTGATCCT GAAAATCCTC ATCCTCATCC TCCTTCTCCT CATGGTGGGG 
CTTTTCCTTT ACTCTTTGCC TGGATACCTG GTAAAGAAGA TCCTGGGAGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

otof ( XM_031901883.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757743.1 Xenopus tropicalis otoferlin (otof), transcript variant X4, mRNA.

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CloneID	OXa58171
Clone ID Related Accession (Same CDS sequence)	XM_031901883.1
Accession Version	XM_031901883.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4710bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	otoferlin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACACCA GCATCATGGC CAACGTCAAG AAAGCTCTCA TTGGAGAAAA CAAGGACTTG 
GTGGACCCCT ATGTGCAAGT TGCCTTTGCA GGGCAGAAGG GAAAGACCTC GGTGCAGAAG 
AGCAGCTACG AGCCCATGTG GAACGAGCAG ATCATTTTCA CCGAGATGTT CCCCCCCCTG 
TGCAAGAGGA TGAAAGTGCA GATCAGAGAT TCCGACAAGG TCAACGATGT CGCCATTGGG 
ACCCACTTCA TTGACCTGAG GAAAATTGCC AACGAGGGGG ACAAGGGATT CCTCCCTACT 
TTCGGCCCAG CCTGGGTGAA TATGTATGGC TCCACCCGTA ACTACACCCT CATGGATGAG 
CACCAGGATC TGAATGAGGG GCTCGGAGAA GGCGTCTCTT TCCGCGCAAG GCTGCTGCTG 
AGTCTCGCTG TTGAGATACT GGATACAACC AACCCAGAGC TCACCAGTTC CACAGAGGTT 
CAAGTTGAAG GAGCTCAACC AGTGGCAGAA ACCTGTACCG GTAAAATGGA AGAGTTCTTC 
CTGTTTGGAG CATTCCTGGA ATCCACCATG ATTGATCGGA AAATTGGGGA CAAGCCAATT 
AACTTTGAGG TCACCATAGG TAACTATGGC AACCAAATAG ATGGAATTTC CAAGCCGAGT 
ATGAGGAAGA GGAAGGAAGG AGGTGACGGA GTCGAGGAGG AGACGGAGCT CTTGCAGAAT 
TCCAGTGATG ATGAGGCTGA TGAAGATGGA GAAATGTTAT CAATCTCTTC GTCCCCTGCA 
ATGAAGCCAC TCATAACTGA TAGGAACTAC TTCCACCTCC CGTTCTTTGA TAAGAAGCCC 
TGTACCTACA TTAAGAGTTG GTGGCAGGAC CAGCGGAGGC GGCTGTACAA TGCCAACATT 
ATGGACAAGA TCGCAGATAA GCTGGAGGAA GGCTTGAACG ATGTTCATGA GATGATAAAG 
ACGGAGAAGC CCTACCCTGA ACGGCGGCTG CGAGGAGTCC TGGAGGAGCT GAGTAGCAGC 
TGTTTTCGAT TTGTTACCTT GGCCAACAAG GACCAGAACC AGAATGGGCA GACGAAACTG 
GACAGGGAGA GGCTGAAGTC CTGCATGAGG GAGCTGGAGA ACATGGGGAC ACAAGCCAAG 
ACCATGAGGT CCCAAGTGAA GAAGAACACG GTTAAGGACA AGCTGAAACT GGTGCAGAAT 
TTCCTGCAGA AGCTCCGGTT CCTGGCAGAT GAGCCCCAGC ACAGCATTCC TGACATCTTC 
ATCTGGATGA TGTCCAACAA CAAGCGCATC GCCTACGCTC GAATCCCTTC CAAAGATATC 
CTGTACTCCA TTGTGGATGA GGAGACGGGA AAGGACTGTG GCAAAGTCAA GTGTGTCTTC 
CTTAAGCTGC CGGGCAAGAG GGGCTTTGGG CCTGCAGGCT GGACGGTACA AGCCAAGATG 
GAGGTCTACA TGTGGTTGGG CCTTAGCAAG CAGCGCAGGG ATTTCTTGTG TGGTCTTCCA 
TGTGGATTTG AGGAGATAAA AGCAGTGAAA GGGCCCGGTC TGCAGTCCTT CCCACCCATC 
AGCCTCAGCT ACACCAAAAA ACAAGTGTTC CAGTTACGAG CCCACATGTA CCAAGCCCGG 
AGTCTCTTTG CCGCTGACAG CAGTGGACTT TCCGACCCCT TTGCCCGCGT CTTCTTCAAC 
ACTCAGAGCC AATGTACAGA GGTGCTGAAT GAGACCCTTT GTCCGACTTG GGACCAGCTG 
TTGGTGTTTG ACAATATCGA ACTCTACGGA GAAGCTCACG AGATGAGAGA CGACCCCCCA 
ATCATTGTCA TTGAGATTTA TGATCAAGAC ACAGTAGGGA AGGCCGATTT CATGGGGAGA 
ACATTTGCCA AACCTGTTGT GAAAATGTCA GATGAGCGGT ACTGCCCTCC CCGCTTTCCG 
CCACAGCTGG AATACTACCA GATCTACCGT GGGAACTCAA CCGCAGGGGA CCTCTTGGGA 
GCTTTCGAAC TTCTCCAGAT CGGACCAGCG GGCAAGTCTG ACCTCCCACA GATTGAAGGA 
CCAACAGACA TAGAGAGAGG ACCCATCCTT CCAGTTCCTC TTGGAATCAG ACCAGTGCTC 
AGCAAATACA GAGTAGAGGT CCTTTTCTGG GGGCTGAGAG ACCTGAAGAG AGTAAATCTA 
GCCCAGGTTG ATAGACCAAG GGTGGATATT GAGTGTGCCG GGAGGGGGGT GCAGTCTGCC 
CTCATCCAGA ACTACAAGAA GAATCCAAAT TTTAGCACGT TGGTCAAATG GTTTGAAGTG 
GATCTCCCCG AGAATGAGTT ACTTCACCCG CCATTGAATA TCCGAGTAGT AGACTGCCGA 
GCCTTTGGCA GATACACTCT GGTTGGTTCC CATGCTGTCA GCTCCCTACG CAGGTTCATT 
TACCGGCCTC CGGATAAGAA CTCCCAGCAG TGGACCACAG CAGCTAAACT CATGAATGGC 
TACTTGGCAA TGACCAATGG AAGGCCCCGC TCTCGCACCA CAGGTGAAAT AGTTATCAAC 
ATGGAGCCCG AAGCGCCCGT CAAGAAGATG GAGACAATGG TGAAACTGGA AGCCAATTCC 
GACGCGGTGG TTAAAGTAGA CGTGAGCGAG GAAGAAAAAG ACAAGAAGAA GAAGAAGAAG 
AAAGGAGTAG AGGAGATAGA GGAAGAGGAG CCAGATGAGA GCATGTTGGA CTGGTGGTCC 
AAATACTTTG CTTCCATTGA AACCATGAAG GAGCAACTGC GCCAACAGGA AGCGGCAGCC 
GCCGAAGCCG AAGAGAAGGA AGAGATGGAG ATCGCAGAGG GCAACAAGGG CCCCACGAAG 
AACAAGGAGA AGGTCAAAGC CCCAAAAGAG GAGAAGAAGA ACAAGAAGCA GCAACAGCAG 
CAGCAACAAG CCGATCAGGC CGAGAAGAAG AACAAGCAAA AGATCGATGA GTTAAAGGTG 
TACAACAAGG AGTTGGAGAC CGAGTATGAC AACTTTGAGG ACTGGCTCCA TACATTTAAC 
TTGTTACGAG GGAAGATTGG AGATGATGAC GATACTGTAA GCGAAGAAGA GCGAATTGTT 
GGGAGGTTTA AGGGTTCCAT GTGTGTGTAC AAAGTTCCTT TGCCAGAAGA CGTCACAAAG 
GAAGCCGGGT ATGACCCAAA CTTCGGCATG TTCCAAGGAA TCCCTAACAA CGACCCAATG 
AATGTGCTGG TCAGAGTTTA TGTCGTGAGG GCCACCGATC TTCATCCAGC TGATATCAAT 
GGTAAAGCGG ATCCATATGT TGTCATAAAA CTAGGAAAAA CTGATATAAA AGATAAGGAA 
AACTACATCT CCAAGCAGCT AAACCCTGTC TTCGGAAAGT CCTTTGATAT TGAGGCCACT 
TTTCCGATGG AGTCCATGCT TACAGTTGCG GTTTATGACT GGGATCTGGT GGGGACGGAT 
GATCTGATCG GTGAAACCAA AATCGACCTG GAAAACCGTT ACTACAGCAA GCACAGGGCG 
ACTTGTGGTA TCTCAAAGAC ATATGCCATT CACGGGTACA ACTTGTGGAG GGACCCAGTG 
AAGCCTACAC AAATCTTATC CAAACTTTGC AAAGAAGGGA AGATCGATGG ACCTCATTAT 
GGCCCTGGTG GAAGAGTGAA AGTCACGAAT CGGGTATTCA CTGGTCCCAC AGAAATTGAT 
GATGAAAATG GTCAGAAGAA ACAAACGGAT GAGCACCTTG CACTCTCGGT CCTTCAGCAC 
TGGGAGGACA TTCCTCGGGT GGGATGTAAG CTGGTCCCAG AGCATGTAGA AACGAGGCCT 
TTGTTAAACC CAGACAAACC TGGAATTGAG CAGGGCCGCA TAGAAATGTG GGTAGACATG 
TTCCCCATGG ACATGCCTGC GCCAGGTCCT GCCATTGATA TCTCTCCAAG GAAGCCAAAA 
AGATACGAGC TTAGAGTGAT AATATGGAAT ACAGATGAGG TAATCCTGGA AGATGATGAT 
TACTTCACAG GAGAAAAATC TAGTGACATA TTTGTCAGGG GGTGGCTGAA GGGACAGCAG 
GAAGACAAAC AAGACACGGA TGTCCACTAT CATTCCCTTA CTGGGGAGGG GAACTTCAAC 
TGGAGGTTCT TGTTCCCTTT TGATTACCTT ATGGCAGAGG AGAAGATTGT CATCTCCAAG 
AAAGAGTCCA TGTTCTCTTG GGATGAGACC GAGTACAAGA TCCCGGCTCG TCTCACTCTC 
CAGGTCTGGG ATGCCGACCA CTTCTCAGCA GATGACTTTC TCGGAGCCAT AGAATTAGAC 
TTGAATCGCT TTCCCCGAGG GGCAAAAACA GCGAAACAGT GTTCGATAGA AATGGCGGAA 
CCCGACGTCG ATCTGCCAAT GGTCTCAATC TTCAAACAGA AGAGGGTGAA GGGATGGTGG 
CCTTTCGTTG CCAGGAATGA AAACGATGAG ATGGAGCTGA CTGGTAAAGT GGAGGCGGAG 
CTTCACCTTC TCTCAGCAGA GGAGGCAGAG AAGACCCCGG CAGGGCTTGG GCGCAATGAA 
CCAGATCCTC TTGAAAAACC AAATCGGCCG AATCCCAGCC TGGCGTGGAT GCTGAGCCCC 
CTGCGGGTAG TGTGCATGTT GCTATGGCGA CAGTACCGGT GGCACGTACT GGGCATCATC 
GGTTCCTGTG CGGTTCTGAT GTTCGTGGCG TTGGTGCTCT ACACCCTCCC CGGCGCTATC 
AGCGAGAGGC TCATTAACCC CCGCGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757743.1
CDS	97..4806
Translation	MNTSIMANVKKALIGENKDLVDPYVQVAFAGQKGKTSVQKSSYEPMWNEQIIFTEMFPPLCKRMKVQIRDSDKVNDVAIGTHFIDLRKIANEGDKGFLPTFGPAWVNMYGSTRNYTLMDEHQDLNEGLGEGVSFRARLLLSLAVEILDTTNPELTSSTEVQVEGAQPVAETCTGKMEEFFLFGAFLESTMIDRKIGDKPINFEVTIGNYGNQIDGISKPSMRKRKEGGDGVEEETELLQNSSDDEADEDGEMLSISSSPAMKPLITDRNYFHLPFFDKKPCTYIKSWWQDQRRRLYNANIMDKIADKLEEGLNDVHEMIKTEKPYPERRLRGVLEELSSSCFRFVTLANKDQNQNGQTKLDRERLKSCMRELENMGTQAKTMRSQVKKNTVKDKLKLVQNFLQKLRFLADEPQHSIPDIFIWMMSNNKRIAYARIPSKDILYSIVDEETGKDCGKVKCVFLKLPGKRGFGPAGWTVQAKMEVYMWLGLSKQRRDFLCGLPCGFEEIKAVKGPGLQSFPPISLSYTKKQVFQLRAHMYQARSLFAADSSGLSDPFARVFFNTQSQCTEVLNETLCPTWDQLLVFDNIELYGEAHEMRDDPPIIVIEIYDQDTVGKADFMGRTFAKPVVKMSDERYCPPRFPPQLEYYQIYRGNSTAGDLLGAFELLQIGPAGKSDLPQIEGPTDIERGPILPVPLGIRPVLSKYRVEVLFWGLRDLKRVNLAQVDRPRVDIECAGRGVQSALIQNYKKNPNFSTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHAVSSLRRFIYRPPDKNSQQWTTAAKLMNGYLAMTNGRPRSRTTGEIVINMEPEAPVKKMETMVKLEANSDAVVKVDVSEEEKDKKKKKKKGVEEIEEEEPDESMLDWWSKYFASIETMKEQLRQQEAAAAEAEEKEEMEIAEGNKGPTKNKEKVKAPKEEKKNKKQQQQQQQADQAEKKNKQKIDELKVYNKELETEYDNFEDWLHTFNLLRGKIGDDDDTVSEEERIVGRFKGSMCVYKVPLPEDVTKEAGYDPNFGMFQGIPNNDPMNVLVRVYVVRATDLHPADINGKADPYVVIKLGKTDIKDKENYISKQLNPVFGKSFDIEATFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRYYSKHRATCGISKTYAIHGYNLWRDPVKPTQILSKLCKEGKIDGPHYGPGGRVKVTNRVFTGPTEIDDENGQKKQTDEHLALSVLQHWEDIPRVGCKLVPEHVETRPLLNPDKPGIEQGRIEMWVDMFPMDMPAPGPAIDISPRKPKRYELRVIIWNTDEVILEDDDYFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRFLFPFDYLMAEEKIVISKKESMFSWDETEYKIPARLTLQVWDADHFSADDFLGAIELDLNRFPRGAKTAKQCSIEMAEPDVDLPMVSIFKQKRVKGWWPFVARNENDEMELTGKVEAELHLLSAEEAEKTPAGLGRNEPDPLEKPNRPNPSLAWMLSPLRVVCMLLWRQYRWHVLGIIGSCAVLMFVALVLYTLPGAISERLINPRG

Target ORF information:

RefSeq Version	XM_031901883.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis otoferlin (otof), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901883.1

ORF Insert Sequence:

ATGAACACCA GCATCATGGC CAACGTCAAG AAAGCTCTCA TTGGAGAAAA CAAGGACTTG 
GTGGACCCCT ATGTGCAAGT TGCCTTTGCA GGGCAGAAGG GAAAGACCTC GGTGCAGAAG 
AGCAGCTACG AGCCCATGTG GAACGAGCAG ATCATTTTCA CCGAGATGTT CCCCCCCCTG 
TGCAAGAGGA TGAAAGTGCA GATCAGAGAT TCCGACAAGG TCAACGATGT CGCCATTGGG 
ACCCACTTCA TTGACCTGAG GAAAATTGCC AACGAGGGGG ACAAGGGATT CCTCCCTACT 
TTCGGCCCAG CCTGGGTGAA TATGTATGGC TCCACCCGTA ACTACACCCT CATGGATGAG 
CACCAGGATC TGAATGAGGG GCTCGGAGAA GGCGTCTCTT TCCGCGCAAG GCTGCTGCTG 
AGTCTCGCTG TTGAGATACT GGATACAACC AACCCAGAGC TCACCAGTTC CACAGAGGTT 
CAAGTTGAAG GAGCTCAACC AGTGGCAGAA ACCTGTACCG GTAAAATGGA AGAGTTCTTC 
CTGTTTGGAG CATTCCTGGA ATCCACCATG ATTGATCGGA AAATTGGGGA CAAGCCAATT 
AACTTTGAGG TCACCATAGG TAACTATGGC AACCAAATAG ATGGAATTTC CAAGCCGAGT 
ATGAGGAAGA GGAAGGAAGG AGGTGACGGA GTCGAGGAGG AGACGGAGCT CTTGCAGAAT 
TCCAGTGATG ATGAGGCTGA TGAAGATGGA GAAATGTTAT CAATCTCTTC GTCCCCTGCA 
ATGAAGCCAC TCATAACTGA TAGGAACTAC TTCCACCTCC CGTTCTTTGA TAAGAAGCCC 
TGTACCTACA TTAAGAGTTG GTGGCAGGAC CAGCGGAGGC GGCTGTACAA TGCCAACATT 
ATGGACAAGA TCGCAGATAA GCTGGAGGAA GGCTTGAACG ATGTTCATGA GATGATAAAG 
ACGGAGAAGC CCTACCCTGA ACGGCGGCTG CGAGGAGTCC TGGAGGAGCT GAGTAGCAGC 
TGTTTTCGAT TTGTTACCTT GGCCAACAAG GACCAGAACC AGAATGGGCA GACGAAACTG 
GACAGGGAGA GGCTGAAGTC CTGCATGAGG GAGCTGGAGA ACATGGGGAC ACAAGCCAAG 
ACCATGAGGT CCCAAGTGAA GAAGAACACG GTTAAGGACA AGCTGAAACT GGTGCAGAAT 
TTCCTGCAGA AGCTCCGGTT CCTGGCAGAT GAGCCCCAGC ACAGCATTCC TGACATCTTC 
ATCTGGATGA TGTCCAACAA CAAGCGCATC GCCTACGCTC GAATCCCTTC CAAAGATATC 
CTGTACTCCA TTGTGGATGA GGAGACGGGA AAGGACTGTG GCAAAGTCAA GTGTGTCTTC 
CTTAAGCTGC CGGGCAAGAG GGGCTTTGGG CCTGCAGGCT GGACGGTACA AGCCAAGATG 
GAGGTCTACA TGTGGTTGGG CCTTAGCAAG CAGCGCAGGG ATTTCTTGTG TGGTCTTCCA 
TGTGGATTTG AGGAGATAAA AGCAGTGAAA GGGCCCGGTC TGCAGTCCTT CCCACCCATC 
AGCCTCAGCT ACACCAAAAA ACAAGTGTTC CAGTTACGAG CCCACATGTA CCAAGCCCGG 
AGTCTCTTTG CCGCTGACAG CAGTGGACTT TCCGACCCCT TTGCCCGCGT CTTCTTCAAC 
ACTCAGAGCC AATGTACAGA GGTGCTGAAT GAGACCCTTT GTCCGACTTG GGACCAGCTG 
TTGGTGTTTG ACAATATCGA ACTCTACGGA GAAGCTCACG AGATGAGAGA CGACCCCCCA 
ATCATTGTCA TTGAGATTTA TGATCAAGAC ACAGTAGGGA AGGCCGATTT CATGGGGAGA 
ACATTTGCCA AACCTGTTGT GAAAATGTCA GATGAGCGGT ACTGCCCTCC CCGCTTTCCG 
CCACAGCTGG AATACTACCA GATCTACCGT GGGAACTCAA CCGCAGGGGA CCTCTTGGGA 
GCTTTCGAAC TTCTCCAGAT CGGACCAGCG GGCAAGTCTG ACCTCCCACA GATTGAAGGA 
CCAACAGACA TAGAGAGAGG ACCCATCCTT CCAGTTCCTC TTGGAATCAG ACCAGTGCTC 
AGCAAATACA GAGTAGAGGT CCTTTTCTGG GGGCTGAGAG ACCTGAAGAG AGTAAATCTA 
GCCCAGGTTG ATAGACCAAG GGTGGATATT GAGTGTGCCG GGAGGGGGGT GCAGTCTGCC 
CTCATCCAGA ACTACAAGAA GAATCCAAAT TTTAGCACGT TGGTCAAATG GTTTGAAGTG 
GATCTCCCCG AGAATGAGTT ACTTCACCCG CCATTGAATA TCCGAGTAGT AGACTGCCGA 
GCCTTTGGCA GATACACTCT GGTTGGTTCC CATGCTGTCA GCTCCCTACG CAGGTTCATT 
TACCGGCCTC CGGATAAGAA CTCCCAGCAG TGGACCACAG CAGCTAAACT CATGAATGGC 
TACTTGGCAA TGACCAATGG AAGGCCCCGC TCTCGCACCA CAGGTGAAAT AGTTATCAAC 
ATGGAGCCCG AAGCGCCCGT CAAGAAGATG GAGACAATGG TGAAACTGGA AGCCAATTCC 
GACGCGGTGG TTAAAGTAGA CGTGAGCGAG GAAGAAAAAG ACAAGAAGAA GAAGAAGAAG 
AAAGGAGTAG AGGAGATAGA GGAAGAGGAG CCAGATGAGA GCATGTTGGA CTGGTGGTCC 
AAATACTTTG CTTCCATTGA AACCATGAAG GAGCAACTGC GCCAACAGGA AGCGGCAGCC 
GCCGAAGCCG AAGAGAAGGA AGAGATGGAG ATCGCAGAGG GCAACAAGGG CCCCACGAAG 
AACAAGGAGA AGGTCAAAGC CCCAAAAGAG GAGAAGAAGA ACAAGAAGCA GCAACAGCAG 
CAGCAACAAG CCGATCAGGC CGAGAAGAAG AACAAGCAAA AGATCGATGA GTTAAAGGTG 
TACAACAAGG AGTTGGAGAC CGAGTATGAC AACTTTGAGG ACTGGCTCCA TACATTTAAC 
TTGTTACGAG GGAAGATTGG AGATGATGAC GATACTGTAA GCGAAGAAGA GCGAATTGTT 
GGGAGGTTTA AGGGTTCCAT GTGTGTGTAC AAAGTTCCTT TGCCAGAAGA CGTCACAAAG 
GAAGCCGGGT ATGACCCAAA CTTCGGCATG TTCCAAGGAA TCCCTAACAA CGACCCAATG 
AATGTGCTGG TCAGAGTTTA TGTCGTGAGG GCCACCGATC TTCATCCAGC TGATATCAAT 
GGTAAAGCGG ATCCATATGT TGTCATAAAA CTAGGAAAAA CTGATATAAA AGATAAGGAA 
AACTACATCT CCAAGCAGCT AAACCCTGTC TTCGGAAAGT CCTTTGATAT TGAGGCCACT 
TTTCCGATGG AGTCCATGCT TACAGTTGCG GTTTATGACT GGGATCTGGT GGGGACGGAT 
GATCTGATCG GTGAAACCAA AATCGACCTG GAAAACCGTT ACTACAGCAA GCACAGGGCG 
ACTTGTGGTA TCTCAAAGAC ATATGCCATT CACGGGTACA ACTTGTGGAG GGACCCAGTG 
AAGCCTACAC AAATCTTATC CAAACTTTGC AAAGAAGGGA AGATCGATGG ACCTCATTAT 
GGCCCTGGTG GAAGAGTGAA AGTCACGAAT CGGGTATTCA CTGGTCCCAC AGAAATTGAT 
GATGAAAATG GTCAGAAGAA ACAAACGGAT GAGCACCTTG CACTCTCGGT CCTTCAGCAC 
TGGGAGGACA TTCCTCGGGT GGGATGTAAG CTGGTCCCAG AGCATGTAGA AACGAGGCCT 
TTGTTAAACC CAGACAAACC TGGAATTGAG CAGGGCCGCA TAGAAATGTG GGTAGACATG 
TTCCCCATGG ACATGCCTGC GCCAGGTCCT GCCATTGATA TCTCTCCAAG GAAGCCAAAA 
AGATACGAGC TTAGAGTGAT AATATGGAAT ACAGATGAGG TAATCCTGGA AGATGATGAT 
TACTTCACAG GAGAAAAATC TAGTGACATA TTTGTCAGGG GGTGGCTGAA GGGACAGCAG 
GAAGACAAAC AAGACACGGA TGTCCACTAT CATTCCCTTA CTGGGGAGGG GAACTTCAAC 
TGGAGGTTCT TGTTCCCTTT TGATTACCTT ATGGCAGAGG AGAAGATTGT CATCTCCAAG 
AAAGAGTCCA TGTTCTCTTG GGATGAGACC GAGTACAAGA TCCCGGCTCG TCTCACTCTC 
CAGGTCTGGG ATGCCGACCA CTTCTCAGCA GATGACTTTC TCGGAGCCAT AGAATTAGAC 
TTGAATCGCT TTCCCCGAGG GGCAAAAACA GCGAAACAGT GTTCGATAGA AATGGCGGAA 
CCCGACGTCG ATCTGCCAAT GGTCTCAATC TTCAAACAGA AGAGGGTGAA GGGATGGTGG 
CCTTTCGTTG CCAGGAATGA AAACGATGAG ATGGAGCTGA CTGGTAAAGT GGAGGCGGAG 
CTTCACCTTC TCTCAGCAGA GGAGGCAGAG AAGACCCCGG CAGGGCTTGG GCGCAATGAA 
CCAGATCCTC TTGAAAAACC AAATCGGCCG AATCCCAGCC TGGCGTGGAT GCTGAGCCCC 
CTGCGGGTAG TGTGCATGTT GCTATGGCGA CAGTACCGGT GGCACGTACT GGGCATCATC 
GGTTCCTGTG CGGTTCTGAT GTTCGTGGCG TTGGTGCTCT ACACCCTCCC CGGCGCTATC 
AGCGAGAGGC TCATTAACCC CCGCGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

otof ( XM_031901884.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757744.1 Xenopus tropicalis otoferlin (otof), transcript variant X5, mRNA.

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CloneID	OXa58172
Clone ID Related Accession (Same CDS sequence)	XM_031901884.1
Accession Version	XM_031901884.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	otoferlin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACACCA GCATCATGGC CAACGTCAAG AAAGCTCTCA TTGGAGAAAA CAAGGACTTG 
GTGGACCCCT ATGTGCAAGT TGCCTTTGCA GGGCAGAAGG GAAAGACCTC GGTGCAGAAG 
AGCAGCTACG AGCCCATGTG GAACGAGCAG ATCATTTTCA CCGAGATGTT CCCCCCCCTG 
TGCAAGAGGA TGAAAGTGCA GATCAGAGAT TCCGACAAGG TCAACGATGT CGCCATTGGG 
ACCCACTTCA TTGACCTGAG GAAAATTGCC AACGAGGGGG ACAAGGGATT CCTCCCTACT 
TTCGGCCCAG CCTGGGTGAA TATGTATGGC TCCACCCGTA ACTACACCCT CATGGATGAG 
CACCAGGATC TGAATGAGGG GCTCGGAGAA GGCGTCTCTT TCCGCGCAAG GCTGCTGCTG 
AGTCTCGCTG TTGAGATACT GGATACAACC AACCCAGAGC TCACCAGTTC CACAGAGGTT 
CAAGTTGAAG GAGCTCAACC AGTGGCAGAA ACCTGTACCG GTAAAATGGA AGAGTTCTTC 
CTGTTTGGAG CATTCCTGGA ATCCACCATG ATTGATCGGA AAATTGGGGA CAAGCCAATT 
AACTTTGAGG TCACCATAGG TAACTATGGC AACCAAATAG ATGGAATTTC CAAGCCGAGT 
ATGAGGAAGA GGAAGGAAGG AGGTGACGGA GTCGAGGAGG AGACGGAGCT CTTGCAGAAT 
TCCAGTGATG ATGAGGCTGA TGAAGATGGA GAAATGTTAT CAATCTCTTC GTCCCCTGCA 
ATGAAGCCAC TCATAACTGA TAGGAACTAC TTCCACCTCC CGTTCTTTGA TAAGAAGCCC 
TGTACCTACA TTAAGAGTTG GTGGCAGGAC CAGCGGAGGC GGCTGTACAA TGCCAACATT 
ATGGACAAGA TCGCAGATAA GCTGGAGGAA GGCTTGAACG ATGTTCATGA GATGATAAAG 
ACGGAGAAGC CCTACCCTGA ACGGCGGCTG CGAGGAGTCC TGGAGGAGCT GAGTAGCAGC 
TGTTTTCGAT TTGTTACCTT GGCCAACAAG GACCAGAACC AGAATGGGCA GACGAAACTG 
GACAGGGAGA GGCTGAAGTC CTGCATGAGG GAGCTGGAGA ACATGGGGAC ACAAGCCAAG 
ACCATGAGGT CCCAAGTGAA GAAGAACACG GTTAAGGACA AGCTGAAACT GGTGCAGAAT 
TTCCTGCAGA AGCTCCGGTT CCTGGCAGAT GAGCCCCAGC ACAGCATTCC TGACATCTTC 
ATCTGGATGA TGTCCAACAA CAAGCGCATC GCCTACGCTC GAATCCCTTC CAAAGATATC 
CTGTACTCCA TTGTGGATGA GGAGACGGGA AAGGACTGTG GCAAAGTCAA GTGTGTCTTC 
CTTAAGCTGC CGGGCAAGAG GGGCTTTGGG CCTGCAGGCT GGACGGTACA AGCCAAGATG 
GAGGTCTACA TGTGGTTGGG CCTTAGCAAG CAGCGCAGGG ATTTCTTGTG TGGTCTTCCA 
TGTGGATTTG AGGAGATAAA AGCAGTGAAA GGGCCCGGTC TGCAGTCCTT CCCACCCATC 
AGCCTCAGCT ACACCAAAAA ACAAGTGTTC CAGTTACGAG CCCACATGTA CCAAGCCCGG 
AGTCTCTTTG CCGCTGACAG CAGTGGACTT TCCGACCCCT TTGCCCGCGT CTTCTTCAAC 
ACTCAGAGCC AATGTACAGA GGTGCTGAAT GAGACCCTTT GTCCGACTTG GGACCAGCTG 
TTGGTGTTTG ACAATATCGA ACTCTACGGA GAAGCTCACG AGATGAGAGA CGACCCCCCA 
ATCATTGTCA TTGAGATTTA TGATCAAGAC ACAGTAGGGA AGGCCGATTT CATGGGGAGA 
ACATTTGCCA AACCTGTTGT GAAAATGTCA GATGAGCGGT ACTGCCCTCC CCGCTTTCCG 
CCACAGCTGG AATACTACCA GATCTACCGT GGGAACTCAA CCGCAGGGGA CCTCTTGGGA 
GCTTTCGAAC TTCTCCAGAT CGGACCAGCG GGCAAGTCTG ACCTCCCACA GATTGAAGGA 
CCAACAGACA TAGAGAGAGG ACCCATCCTT CCAGTTCCTC TTGGAATCAG ACCAGTGCTC 
AGCAAATACA GAGTAGAGGT CCTTTTCTGG GGGCTGAGAG ACCTGAAGAG AGTAAATCTA 
GCCCAGGTTG ATAGACCAAG GGTGGATATT GAGTGTGCCG GGAGGGGGGT GCAGTCTGCC 
CTCATCCAGA ACTACAAGAA GAATCCAAAT TTTAGCACGT TGGTCAAATG GTTTGAAGTG 
GATCTCCCCG AGAATGAGTT ACTTCACCCG CCATTGAATA TCCGAGTAGT AGACTGCCGA 
GCCTTTGGCA GATACACTCT GGTTGGTTCC CATGCTGTCA GCTCCCTACG CAGGTTCATT 
TACCGGCCTC CGGATAAGAA CTCCCAGCAG TGGACCACAG CAGGTGAAAT AGTTATCAAC 
ATGGAGCCCG AAGCGCCCGT CAAGAAGATG GAGACAATGG TGAAACTGGA AGCCAATTCC 
GACGCGGTGG TTAAAGTAGA CGTGAGCGAG GAAGAAAAAG ACAAGAAGAA GAAGAAGAAG 
AAAGGAGTAG AGGAGATAGA GGAAGAGGAG CCAGATGAGA GCATGTTGGA CTGGTGGTCC 
AAATACTTTG CTTCCATTGA AACCATGAAG GAGCAACTGC GCCAACAGGA AGCGGCAGCC 
GCCGAAGCCG AAGAGAAGGA AGAGATGGAG ATCGCAGAGG AGATCAAAAC TGATGACTCT 
CCCATGAAAG GCAACAAGGG CCCCACGAAG AACAAGGAGA AGGTCAAAGC CCCAAAAGAG 
GAGAAGAAGA ACAAGAAGCA GCAACAGCAG CAGCAACAAG CCGATCAGGC CGAGAAGAAG 
AACAAGCAAA AGATCGATGA GTTAAAGGTG TACAACAAGG AGTTGGAGAC CGAGTATGAC 
AACTTTGAGG ACTGGCTCCA TACATTTAAC TTGTTACGAG GGAAGATTGG AGATGATGAC 
GATACTGTAA GCGAAGAAGA GCGAATTGTT GGGAGGTTTA AGGGTTCCAT GTGTGTGTAC 
AAAGTTCCTT TGCCAGAAGA CGTCACAAAG GAAGCCGGGT ATGACCCAAA CTTCGGCATG 
TTCCAAGGAA TCCCTAACAA CGACCCAATG AATGTGCTGG TCAGAGTTTA TGTCGTGAGG 
GCCACCGATC TTCATCCAGC TGATATCAAT GGTAAAGCGG ATCCATATGT TGTCATAAAA 
CTAGGAAAAA CTGATATAAA AGATAAGGAA AACTACATCT CCAAGCAGCT AAACCCTGTC 
TTCGGAAAGT CCTTTGATAT TGAGGCCACT TTTCCGATGG AGTCCATGCT TACAGTTGCG 
GTTTATGACT GGGATCTGGT GGGGACGGAT GATCTGATCG GTGAAACCAA AATCGACCTG 
GAAAACCGTT ACTACAGCAA GCACAGGGCG ACTTGTGGTA TCTCAAAGAC ATATGCCATT 
CACGGGTACA ACTTGTGGAG GGACCCAGTG AAGCCTACAC AAATCTTATC CAAACTTTGC 
AAAGAAGGGA AGATCGATGG ACCTCATTAT GGCCCTGGTG GAAGAGTGAA AGTCACGAAT 
CGGGTATTCA CTGGTCCCAC AGAAATTGAT GATGAAAATG GTCAGAAGAA ACAAACGGAT 
GAGCACCTTG CACTCTCGGT CCTTCAGCAC TGGGAGGACA TTCCTCGGGT GGGATGTAAG 
CTGGTCCCAG AGCATGTAGA AACGAGGCCT TTGTTAAACC CAGACAAACC TGGAATTGAG 
CAGGGCCGCA TAGAAATGTG GGTAGACATG TTCCCCATGG ACATGCCTGC GCCAGGTCCT 
GCCATTGATA TCTCTCCAAG GAAGCCAAAA AGATACGAGC TTAGAGTGAT AATATGGAAT 
ACAGATGAGG TAATCCTGGA AGATGATGAT TACTTCACAG GAGAAAAATC TAGTGACATA 
TTTGTCAGGG GGTGGCTGAA GGGACAGCAG GAAGACAAAC AAGACACGGA TGTCCACTAT 
CATTCCCTTA CTGGGGAGGG GAACTTCAAC TGGAGGTTCT TGTTCCCTTT TGATTACCTT 
ATGGCAGAGG AGAAGATTGT CATCTCCAAG AAAGAGTCCA TGTTCTCTTG GGATGAGACC 
GAGTACAAGA TCCCGGCTCG TCTCACTCTC CAGGTCTGGG ATGCCGACCA CTTCTCAGCA 
GATGACTTTC TCGGAGCCAT AGAATTAGAC TTGAATCGCT TTCCCCGAGG GGCAAAAACA 
GCGAAACAGT GTTCGATAGA AATGGCGGAA CCCGACGTCG ATCTGCCAAT GGTCTCAATC 
TTCAAACAGA AGAGGGTGAA GGGATGGTGG CCTTTCGTTG CCAGGAATGA AAACGATGAG 
ATGGAGCTGA CTGGTAAAGT GGAGGCGGAG CTTCACCTTC TCTCAGCAGA GGAGGCAGAG 
AAGACCCCGG CAGGGCTTGG GCGCAATGAA CCAGATCCTC TTGAAAAACC AAATCGGCCG 
AATCCCAGCC TGGCGTGGAT GCTGAGCCCC CTGCGGGTAG TGTGCATGTT GCTATGGCGA 
CAGTACCGGT GGCACGTACT GGGCATCATC GGTTCCTGTG CGGTTCTGAT GTTCGTGGCG 
TTGGTGCTCT ACACCCTCCC CGGCGCTATC AGCGAGAGGC TCATTAACCC CCGCGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757744.1
CDS	97..4776
Translation	MNTSIMANVKKALIGENKDLVDPYVQVAFAGQKGKTSVQKSSYEPMWNEQIIFTEMFPPLCKRMKVQIRDSDKVNDVAIGTHFIDLRKIANEGDKGFLPTFGPAWVNMYGSTRNYTLMDEHQDLNEGLGEGVSFRARLLLSLAVEILDTTNPELTSSTEVQVEGAQPVAETCTGKMEEFFLFGAFLESTMIDRKIGDKPINFEVTIGNYGNQIDGISKPSMRKRKEGGDGVEEETELLQNSSDDEADEDGEMLSISSSPAMKPLITDRNYFHLPFFDKKPCTYIKSWWQDQRRRLYNANIMDKIADKLEEGLNDVHEMIKTEKPYPERRLRGVLEELSSSCFRFVTLANKDQNQNGQTKLDRERLKSCMRELENMGTQAKTMRSQVKKNTVKDKLKLVQNFLQKLRFLADEPQHSIPDIFIWMMSNNKRIAYARIPSKDILYSIVDEETGKDCGKVKCVFLKLPGKRGFGPAGWTVQAKMEVYMWLGLSKQRRDFLCGLPCGFEEIKAVKGPGLQSFPPISLSYTKKQVFQLRAHMYQARSLFAADSSGLSDPFARVFFNTQSQCTEVLNETLCPTWDQLLVFDNIELYGEAHEMRDDPPIIVIEIYDQDTVGKADFMGRTFAKPVVKMSDERYCPPRFPPQLEYYQIYRGNSTAGDLLGAFELLQIGPAGKSDLPQIEGPTDIERGPILPVPLGIRPVLSKYRVEVLFWGLRDLKRVNLAQVDRPRVDIECAGRGVQSALIQNYKKNPNFSTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHAVSSLRRFIYRPPDKNSQQWTTAGEIVINMEPEAPVKKMETMVKLEANSDAVVKVDVSEEEKDKKKKKKKGVEEIEEEEPDESMLDWWSKYFASIETMKEQLRQQEAAAAEAEEKEEMEIAEEIKTDDSPMKGNKGPTKNKEKVKAPKEEKKNKKQQQQQQQADQAEKKNKQKIDELKVYNKELETEYDNFEDWLHTFNLLRGKIGDDDDTVSEEERIVGRFKGSMCVYKVPLPEDVTKEAGYDPNFGMFQGIPNNDPMNVLVRVYVVRATDLHPADINGKADPYVVIKLGKTDIKDKENYISKQLNPVFGKSFDIEATFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRYYSKHRATCGISKTYAIHGYNLWRDPVKPTQILSKLCKEGKIDGPHYGPGGRVKVTNRVFTGPTEIDDENGQKKQTDEHLALSVLQHWEDIPRVGCKLVPEHVETRPLLNPDKPGIEQGRIEMWVDMFPMDMPAPGPAIDISPRKPKRYELRVIIWNTDEVILEDDDYFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRFLFPFDYLMAEEKIVISKKESMFSWDETEYKIPARLTLQVWDADHFSADDFLGAIELDLNRFPRGAKTAKQCSIEMAEPDVDLPMVSIFKQKRVKGWWPFVARNENDEMELTGKVEAELHLLSAEEAEKTPAGLGRNEPDPLEKPNRPNPSLAWMLSPLRVVCMLLWRQYRWHVLGIIGSCAVLMFVALVLYTLPGAISERLINPRG

Target ORF information:

RefSeq Version	XM_031901884.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis otoferlin (otof), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901884.1

ORF Insert Sequence:

ATGAACACCA GCATCATGGC CAACGTCAAG AAAGCTCTCA TTGGAGAAAA CAAGGACTTG 
GTGGACCCCT ATGTGCAAGT TGCCTTTGCA GGGCAGAAGG GAAAGACCTC GGTGCAGAAG 
AGCAGCTACG AGCCCATGTG GAACGAGCAG ATCATTTTCA CCGAGATGTT CCCCCCCCTG 
TGCAAGAGGA TGAAAGTGCA GATCAGAGAT TCCGACAAGG TCAACGATGT CGCCATTGGG 
ACCCACTTCA TTGACCTGAG GAAAATTGCC AACGAGGGGG ACAAGGGATT CCTCCCTACT 
TTCGGCCCAG CCTGGGTGAA TATGTATGGC TCCACCCGTA ACTACACCCT CATGGATGAG 
CACCAGGATC TGAATGAGGG GCTCGGAGAA GGCGTCTCTT TCCGCGCAAG GCTGCTGCTG 
AGTCTCGCTG TTGAGATACT GGATACAACC AACCCAGAGC TCACCAGTTC CACAGAGGTT 
CAAGTTGAAG GAGCTCAACC AGTGGCAGAA ACCTGTACCG GTAAAATGGA AGAGTTCTTC 
CTGTTTGGAG CATTCCTGGA ATCCACCATG ATTGATCGGA AAATTGGGGA CAAGCCAATT 
AACTTTGAGG TCACCATAGG TAACTATGGC AACCAAATAG ATGGAATTTC CAAGCCGAGT 
ATGAGGAAGA GGAAGGAAGG AGGTGACGGA GTCGAGGAGG AGACGGAGCT CTTGCAGAAT 
TCCAGTGATG ATGAGGCTGA TGAAGATGGA GAAATGTTAT CAATCTCTTC GTCCCCTGCA 
ATGAAGCCAC TCATAACTGA TAGGAACTAC TTCCACCTCC CGTTCTTTGA TAAGAAGCCC 
TGTACCTACA TTAAGAGTTG GTGGCAGGAC CAGCGGAGGC GGCTGTACAA TGCCAACATT 
ATGGACAAGA TCGCAGATAA GCTGGAGGAA GGCTTGAACG ATGTTCATGA GATGATAAAG 
ACGGAGAAGC CCTACCCTGA ACGGCGGCTG CGAGGAGTCC TGGAGGAGCT GAGTAGCAGC 
TGTTTTCGAT TTGTTACCTT GGCCAACAAG GACCAGAACC AGAATGGGCA GACGAAACTG 
GACAGGGAGA GGCTGAAGTC CTGCATGAGG GAGCTGGAGA ACATGGGGAC ACAAGCCAAG 
ACCATGAGGT CCCAAGTGAA GAAGAACACG GTTAAGGACA AGCTGAAACT GGTGCAGAAT 
TTCCTGCAGA AGCTCCGGTT CCTGGCAGAT GAGCCCCAGC ACAGCATTCC TGACATCTTC 
ATCTGGATGA TGTCCAACAA CAAGCGCATC GCCTACGCTC GAATCCCTTC CAAAGATATC 
CTGTACTCCA TTGTGGATGA GGAGACGGGA AAGGACTGTG GCAAAGTCAA GTGTGTCTTC 
CTTAAGCTGC CGGGCAAGAG GGGCTTTGGG CCTGCAGGCT GGACGGTACA AGCCAAGATG 
GAGGTCTACA TGTGGTTGGG CCTTAGCAAG CAGCGCAGGG ATTTCTTGTG TGGTCTTCCA 
TGTGGATTTG AGGAGATAAA AGCAGTGAAA GGGCCCGGTC TGCAGTCCTT CCCACCCATC 
AGCCTCAGCT ACACCAAAAA ACAAGTGTTC CAGTTACGAG CCCACATGTA CCAAGCCCGG 
AGTCTCTTTG CCGCTGACAG CAGTGGACTT TCCGACCCCT TTGCCCGCGT CTTCTTCAAC 
ACTCAGAGCC AATGTACAGA GGTGCTGAAT GAGACCCTTT GTCCGACTTG GGACCAGCTG 
TTGGTGTTTG ACAATATCGA ACTCTACGGA GAAGCTCACG AGATGAGAGA CGACCCCCCA 
ATCATTGTCA TTGAGATTTA TGATCAAGAC ACAGTAGGGA AGGCCGATTT CATGGGGAGA 
ACATTTGCCA AACCTGTTGT GAAAATGTCA GATGAGCGGT ACTGCCCTCC CCGCTTTCCG 
CCACAGCTGG AATACTACCA GATCTACCGT GGGAACTCAA CCGCAGGGGA CCTCTTGGGA 
GCTTTCGAAC TTCTCCAGAT CGGACCAGCG GGCAAGTCTG ACCTCCCACA GATTGAAGGA 
CCAACAGACA TAGAGAGAGG ACCCATCCTT CCAGTTCCTC TTGGAATCAG ACCAGTGCTC 
AGCAAATACA GAGTAGAGGT CCTTTTCTGG GGGCTGAGAG ACCTGAAGAG AGTAAATCTA 
GCCCAGGTTG ATAGACCAAG GGTGGATATT GAGTGTGCCG GGAGGGGGGT GCAGTCTGCC 
CTCATCCAGA ACTACAAGAA GAATCCAAAT TTTAGCACGT TGGTCAAATG GTTTGAAGTG 
GATCTCCCCG AGAATGAGTT ACTTCACCCG CCATTGAATA TCCGAGTAGT AGACTGCCGA 
GCCTTTGGCA GATACACTCT GGTTGGTTCC CATGCTGTCA GCTCCCTACG CAGGTTCATT 
TACCGGCCTC CGGATAAGAA CTCCCAGCAG TGGACCACAG CAGGTGAAAT AGTTATCAAC 
ATGGAGCCCG AAGCGCCCGT CAAGAAGATG GAGACAATGG TGAAACTGGA AGCCAATTCC 
GACGCGGTGG TTAAAGTAGA CGTGAGCGAG GAAGAAAAAG ACAAGAAGAA GAAGAAGAAG 
AAAGGAGTAG AGGAGATAGA GGAAGAGGAG CCAGATGAGA GCATGTTGGA CTGGTGGTCC 
AAATACTTTG CTTCCATTGA AACCATGAAG GAGCAACTGC GCCAACAGGA AGCGGCAGCC 
GCCGAAGCCG AAGAGAAGGA AGAGATGGAG ATCGCAGAGG AGATCAAAAC TGATGACTCT 
CCCATGAAAG GCAACAAGGG CCCCACGAAG AACAAGGAGA AGGTCAAAGC CCCAAAAGAG 
GAGAAGAAGA ACAAGAAGCA GCAACAGCAG CAGCAACAAG CCGATCAGGC CGAGAAGAAG 
AACAAGCAAA AGATCGATGA GTTAAAGGTG TACAACAAGG AGTTGGAGAC CGAGTATGAC 
AACTTTGAGG ACTGGCTCCA TACATTTAAC TTGTTACGAG GGAAGATTGG AGATGATGAC 
GATACTGTAA GCGAAGAAGA GCGAATTGTT GGGAGGTTTA AGGGTTCCAT GTGTGTGTAC 
AAAGTTCCTT TGCCAGAAGA CGTCACAAAG GAAGCCGGGT ATGACCCAAA CTTCGGCATG 
TTCCAAGGAA TCCCTAACAA CGACCCAATG AATGTGCTGG TCAGAGTTTA TGTCGTGAGG 
GCCACCGATC TTCATCCAGC TGATATCAAT GGTAAAGCGG ATCCATATGT TGTCATAAAA 
CTAGGAAAAA CTGATATAAA AGATAAGGAA AACTACATCT CCAAGCAGCT AAACCCTGTC 
TTCGGAAAGT CCTTTGATAT TGAGGCCACT TTTCCGATGG AGTCCATGCT TACAGTTGCG 
GTTTATGACT GGGATCTGGT GGGGACGGAT GATCTGATCG GTGAAACCAA AATCGACCTG 
GAAAACCGTT ACTACAGCAA GCACAGGGCG ACTTGTGGTA TCTCAAAGAC ATATGCCATT 
CACGGGTACA ACTTGTGGAG GGACCCAGTG AAGCCTACAC AAATCTTATC CAAACTTTGC 
AAAGAAGGGA AGATCGATGG ACCTCATTAT GGCCCTGGTG GAAGAGTGAA AGTCACGAAT 
CGGGTATTCA CTGGTCCCAC AGAAATTGAT GATGAAAATG GTCAGAAGAA ACAAACGGAT 
GAGCACCTTG CACTCTCGGT CCTTCAGCAC TGGGAGGACA TTCCTCGGGT GGGATGTAAG 
CTGGTCCCAG AGCATGTAGA AACGAGGCCT TTGTTAAACC CAGACAAACC TGGAATTGAG 
CAGGGCCGCA TAGAAATGTG GGTAGACATG TTCCCCATGG ACATGCCTGC GCCAGGTCCT 
GCCATTGATA TCTCTCCAAG GAAGCCAAAA AGATACGAGC TTAGAGTGAT AATATGGAAT 
ACAGATGAGG TAATCCTGGA AGATGATGAT TACTTCACAG GAGAAAAATC TAGTGACATA 
TTTGTCAGGG GGTGGCTGAA GGGACAGCAG GAAGACAAAC AAGACACGGA TGTCCACTAT 
CATTCCCTTA CTGGGGAGGG GAACTTCAAC TGGAGGTTCT TGTTCCCTTT TGATTACCTT 
ATGGCAGAGG AGAAGATTGT CATCTCCAAG AAAGAGTCCA TGTTCTCTTG GGATGAGACC 
GAGTACAAGA TCCCGGCTCG TCTCACTCTC CAGGTCTGGG ATGCCGACCA CTTCTCAGCA 
GATGACTTTC TCGGAGCCAT AGAATTAGAC TTGAATCGCT TTCCCCGAGG GGCAAAAACA 
GCGAAACAGT GTTCGATAGA AATGGCGGAA CCCGACGTCG ATCTGCCAAT GGTCTCAATC 
TTCAAACAGA AGAGGGTGAA GGGATGGTGG CCTTTCGTTG CCAGGAATGA AAACGATGAG 
ATGGAGCTGA CTGGTAAAGT GGAGGCGGAG CTTCACCTTC TCTCAGCAGA GGAGGCAGAG 
AAGACCCCGG CAGGGCTTGG GCGCAATGAA CCAGATCCTC TTGAAAAACC AAATCGGCCG 
AATCCCAGCC TGGCGTGGAT GCTGAGCCCC CTGCGGGTAG TGTGCATGTT GCTATGGCGA 
CAGTACCGGT GGCACGTACT GGGCATCATC GGTTCCTGTG CGGTTCTGAT GTTCGTGGCG 
TTGGTGCTCT ACACCCTCCC CGGCGCTATC AGCGAGAGGC TCATTAACCC CCGCGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

otof ( XM_031901885.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757745.1 Xenopus tropicalis otoferlin (otof), transcript variant X6, mRNA.

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CloneID	OXa58173
Clone ID Related Accession (Same CDS sequence)	XM_031901885.1
Accession Version	XM_031901885.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4644bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	otoferlin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACACCA GCATCATGGC CAACGTCAAG AAAGCTCTCA TTGGAGAAAA CAAGGACTTG 
GTGGACCCCT ATGTGCAAGT TGCCTTTGCA GGGCAGAAGG GAAAGACCTC GGTGCAGAAG 
AGCAGCTACG AGCCCATGTG GAACGAGCAG ATCATTTTCA CCGAGATGTT CCCCCCCCTG 
TGCAAGAGGA TGAAAGTGCA GATCAGAGAT TCCGACAAGG TCAACGATGT CGCCATTGGG 
ACCCACTTCA TTGACCTGAG GAAAATTGCC AACGAGGGGG ACAAGGGATT CCTCCCTACT 
TTCGGCCCAG CCTGGGTGAA TATGTATGGC TCCACCCGTA ACTACACCCT CATGGATGAG 
CACCAGGATC TGAATGAGGG GCTCGGAGAA GGCGTCTCTT TCCGCGCAAG GCTGCTGCTG 
AGTCTCGCTG TTGAGATACT GGATACAACC AACCCAGAGC TCACCAGTTC CACAGAGGTT 
CAAGTTGAAG GAGCTCAACC AGTGGCAGAA ACCTGTACCG GTAAAATGGA AGAGTTCTTC 
CTGTTTGGAG CATTCCTGGA ATCCACCATG ATTGATCGGA AAATTGGGGA CAAGCCAATT 
AACTTTGAGG TCACCATAGG TAACTATGGC AACCAAATAG ATGGAATTTC CAAGCCGAGT 
ATGAGGAAGA GGAAGGAAGG AGGTGACGGA GTCGAGGAGG AGACGGAGCT CTTGCAGAAT 
TCCAGTGATG ATGAGGCTGA TGAAGATGGA GAAATGTTAT CAATCTCTTC GTCCCCTGCA 
ATGAAGCCAC TCATAACTGA TAGGAACTAC TTCCACCTCC CGTTCTTTGA TAAGAAGCCC 
TGTACCTACA TTAAGAGTTG GTGGCAGGAC CAGCGGAGGC GGCTGTACAA TGCCAACATT 
ATGGACAAGA TCGCAGATAA GCTGGAGGAA GGCTTGAACG ATGTTCATGA GATGATAAAG 
ACGGAGAAGC CCTACCCTGA ACGGCGGCTG CGAGGAGTCC TGGAGGAGCT GAGTAGCAGC 
TGTTTTCGAT TTGTTACCTT GGCCAACAAG GACCAGAACC AGAATGGGCA GACGAAACTG 
GACAGGGAGA GGCTGAAGTC CTGCATGAGG GAGCTGGAGA ACATGGGGAC ACAAGCCAAG 
ACCATGAGGT CCCAAGTGAA GAAGAACACG GTTAAGGACA AGCTGAAACT GGTGCAGAAT 
TTCCTGCAGA AGCTCCGGTT CCTGGCAGAT GAGCCCCAGC ACAGCATTCC TGACATCTTC 
ATCTGGATGA TGTCCAACAA CAAGCGCATC GCCTACGCTC GAATCCCTTC CAAAGATATC 
CTGTACTCCA TTGTGGATGA GGAGACGGGA AAGGACTGTG GCAAAGTCAA GTGTGTCTTC 
CTTAAGCTGC CGGGCAAGAG GGGCTTTGGG CCTGCAGGCT GGACGGTACA AGCCAAGATG 
GAGGTCTACA TGTGGTTGGG CCTTAGCAAG CAGCGCAGGG ATTTCTTGTG TGGTCTTCCA 
TGTGGATTTG AGGAGATAAA AGCAGTGAAA GGGCCCGGTC TGCAGTCCTT CCCACCCATC 
AGCCTCAGCT ACACCAAAAA ACAAGTGTTC CAGTTACGAG CCCACATGTA CCAAGCCCGG 
AGTCTCTTTG CCGCTGACAG CAGTGGACTT TCCGACCCCT TTGCCCGCGT CTTCTTCAAC 
ACTCAGAGCC AATGTACAGA GGTGCTGAAT GAGACCCTTT GTCCGACTTG GGACCAGCTG 
TTGGTGTTTG ACAATATCGA ACTCTACGGA GAAGCTCACG AGATGAGAGA CGACCCCCCA 
ATCATTGTCA TTGAGATTTA TGATCAAGAC ACAGTAGGGA AGGCCGATTT CATGGGGAGA 
ACATTTGCCA AACCTGTTGT GAAAATGTCA GATGAGCGGT ACTGCCCTCC CCGCTTTCCG 
CCACAGCTGG AATACTACCA GATCTACCGT GGGAACTCAA CCGCAGGGGA CCTCTTGGGA 
GCTTTCGAAC TTCTCCAGAT CGGACCAGCG GGCAAGTCTG ACCTCCCACA GATTGAAGGA 
CCAACAGACA TAGAGAGAGG ACCCATCCTT CCAGTTCCTC TTGGAATCAG ACCAGTGCTC 
AGCAAATACA GAGTAGAGGT CCTTTTCTGG GGGCTGAGAG ACCTGAAGAG AGTAAATCTA 
GCCCAGGTTG ATAGACCAAG GGTGGATATT GAGTGTGCCG GGAGGGGGGT GCAGTCTGCC 
CTCATCCAGA ACTACAAGAA GAATCCAAAT TTTAGCACGT TGGTCAAATG GTTTGAAGTG 
GATCTCCCCG AGAATGAGTT ACTTCACCCG CCATTGAATA TCCGAGTAGT AGACTGCCGA 
GCCTTTGGCA GATACACTCT GGTTGGTTCC CATGCTGTCA GCTCCCTACG CAGGTTCATT 
TACCGGCCTC CGGATAAGAA CTCCCAGCAG TGGACCACAG CAGGTGAAAT AGTTATCAAC 
ATGGAGCCCG AAGCGCCCGT CAAGAAGATG GAGACAATGG TGAAACTGGA AGCCAATTCC 
GACGCGGTGG TTAAAGTAGA CGTGAGCGAG GAAGAAAAAG ACAAGAAGAA GAAGAAGAAG 
AAAGGAGTAG AGGAGATAGA GGAAGAGGAG CCAGATGAGA GCATGTTGGA CTGGTGGTCC 
AAATACTTTG CTTCCATTGA AACCATGAAG GAGCAACTGC GCCAACAGGA AGCGGCAGCC 
GCCGAAGCCG AAGAGAAGGA AGAGATGGAG ATCGCAGAGG GCAACAAGGG CCCCACGAAG 
AACAAGGAGA AGGTCAAAGC CCCAAAAGAG GAGAAGAAGA ACAAGAAGCA GCAACAGCAG 
CAGCAACAAG CCGATCAGGC CGAGAAGAAG AACAAGCAAA AGATCGATGA GTTAAAGGTG 
TACAACAAGG AGTTGGAGAC CGAGTATGAC AACTTTGAGG ACTGGCTCCA TACATTTAAC 
TTGTTACGAG GGAAGATTGG AGATGATGAC GATACTGTAA GCGAAGAAGA GCGAATTGTT 
GGGAGGTTTA AGGGTTCCAT GTGTGTGTAC AAAGTTCCTT TGCCAGAAGA CGTCACAAAG 
GAAGCCGGGT ATGACCCAAA CTTCGGCATG TTCCAAGGAA TCCCTAACAA CGACCCAATG 
AATGTGCTGG TCAGAGTTTA TGTCGTGAGG GCCACCGATC TTCATCCAGC TGATATCAAT 
GGTAAAGCGG ATCCATATGT TGTCATAAAA CTAGGAAAAA CTGATATAAA AGATAAGGAA 
AACTACATCT CCAAGCAGCT AAACCCTGTC TTCGGAAAGT CCTTTGATAT TGAGGCCACT 
TTTCCGATGG AGTCCATGCT TACAGTTGCG GTTTATGACT GGGATCTGGT GGGGACGGAT 
GATCTGATCG GTGAAACCAA AATCGACCTG GAAAACCGTT ACTACAGCAA GCACAGGGCG 
ACTTGTGGTA TCTCAAAGAC ATATGCCATT CACGGGTACA ACTTGTGGAG GGACCCAGTG 
AAGCCTACAC AAATCTTATC CAAACTTTGC AAAGAAGGGA AGATCGATGG ACCTCATTAT 
GGCCCTGGTG GAAGAGTGAA AGTCACGAAT CGGGTATTCA CTGGTCCCAC AGAAATTGAT 
GATGAAAATG GTCAGAAGAA ACAAACGGAT GAGCACCTTG CACTCTCGGT CCTTCAGCAC 
TGGGAGGACA TTCCTCGGGT GGGATGTAAG CTGGTCCCAG AGCATGTAGA AACGAGGCCT 
TTGTTAAACC CAGACAAACC TGGAATTGAG CAGGGCCGCA TAGAAATGTG GGTAGACATG 
TTCCCCATGG ACATGCCTGC GCCAGGTCCT GCCATTGATA TCTCTCCAAG GAAGCCAAAA 
AGATACGAGC TTAGAGTGAT AATATGGAAT ACAGATGAGG TAATCCTGGA AGATGATGAT 
TACTTCACAG GAGAAAAATC TAGTGACATA TTTGTCAGGG GGTGGCTGAA GGGACAGCAG 
GAAGACAAAC AAGACACGGA TGTCCACTAT CATTCCCTTA CTGGGGAGGG GAACTTCAAC 
TGGAGGTTCT TGTTCCCTTT TGATTACCTT ATGGCAGAGG AGAAGATTGT CATCTCCAAG 
AAAGAGTCCA TGTTCTCTTG GGATGAGACC GAGTACAAGA TCCCGGCTCG TCTCACTCTC 
CAGGTCTGGG ATGCCGACCA CTTCTCAGCA GATGACTTTC TCGGAGCCAT AGAATTAGAC 
TTGAATCGCT TTCCCCGAGG GGCAAAAACA GCGAAACAGT GTTCGATAGA AATGGCGGAA 
CCCGACGTCG ATCTGCCAAT GGTCTCAATC TTCAAACAGA AGAGGGTGAA GGGATGGTGG 
CCTTTCGTTG CCAGGAATGA AAACGATGAG ATGGAGCTGA CTGGTAAAGT GGAGGCGGAG 
CTTCACCTTC TCTCAGCAGA GGAGGCAGAG AAGACCCCGG CAGGGCTTGG GCGCAATGAA 
CCAGATCCTC TTGAAAAACC AAATCGACCC GACACCTCGT TCATCTGGTT CCTCAACCCA 
CTTAAGTCCA TCAAGTATCT CATCTGTAAT CGCTACAAGT GGCTCATCAT TAAGATTGTG 
CTGGCGTTGC TGCTTTTGGC CATGGTGGCC CTCTTCCTCT ACAGCATGCC CGGTTACATG 
GTGAAGAAGC TTCTGGGTGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757745.1
CDS	97..4740
Translation	MNTSIMANVKKALIGENKDLVDPYVQVAFAGQKGKTSVQKSSYEPMWNEQIIFTEMFPPLCKRMKVQIRDSDKVNDVAIGTHFIDLRKIANEGDKGFLPTFGPAWVNMYGSTRNYTLMDEHQDLNEGLGEGVSFRARLLLSLAVEILDTTNPELTSSTEVQVEGAQPVAETCTGKMEEFFLFGAFLESTMIDRKIGDKPINFEVTIGNYGNQIDGISKPSMRKRKEGGDGVEEETELLQNSSDDEADEDGEMLSISSSPAMKPLITDRNYFHLPFFDKKPCTYIKSWWQDQRRRLYNANIMDKIADKLEEGLNDVHEMIKTEKPYPERRLRGVLEELSSSCFRFVTLANKDQNQNGQTKLDRERLKSCMRELENMGTQAKTMRSQVKKNTVKDKLKLVQNFLQKLRFLADEPQHSIPDIFIWMMSNNKRIAYARIPSKDILYSIVDEETGKDCGKVKCVFLKLPGKRGFGPAGWTVQAKMEVYMWLGLSKQRRDFLCGLPCGFEEIKAVKGPGLQSFPPISLSYTKKQVFQLRAHMYQARSLFAADSSGLSDPFARVFFNTQSQCTEVLNETLCPTWDQLLVFDNIELYGEAHEMRDDPPIIVIEIYDQDTVGKADFMGRTFAKPVVKMSDERYCPPRFPPQLEYYQIYRGNSTAGDLLGAFELLQIGPAGKSDLPQIEGPTDIERGPILPVPLGIRPVLSKYRVEVLFWGLRDLKRVNLAQVDRPRVDIECAGRGVQSALIQNYKKNPNFSTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHAVSSLRRFIYRPPDKNSQQWTTAGEIVINMEPEAPVKKMETMVKLEANSDAVVKVDVSEEEKDKKKKKKKGVEEIEEEEPDESMLDWWSKYFASIETMKEQLRQQEAAAAEAEEKEEMEIAEGNKGPTKNKEKVKAPKEEKKNKKQQQQQQQADQAEKKNKQKIDELKVYNKELETEYDNFEDWLHTFNLLRGKIGDDDDTVSEEERIVGRFKGSMCVYKVPLPEDVTKEAGYDPNFGMFQGIPNNDPMNVLVRVYVVRATDLHPADINGKADPYVVIKLGKTDIKDKENYISKQLNPVFGKSFDIEATFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRYYSKHRATCGISKTYAIHGYNLWRDPVKPTQILSKLCKEGKIDGPHYGPGGRVKVTNRVFTGPTEIDDENGQKKQTDEHLALSVLQHWEDIPRVGCKLVPEHVETRPLLNPDKPGIEQGRIEMWVDMFPMDMPAPGPAIDISPRKPKRYELRVIIWNTDEVILEDDDYFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRFLFPFDYLMAEEKIVISKKESMFSWDETEYKIPARLTLQVWDADHFSADDFLGAIELDLNRFPRGAKTAKQCSIEMAEPDVDLPMVSIFKQKRVKGWWPFVARNENDEMELTGKVEAELHLLSAEEAEKTPAGLGRNEPDPLEKPNRPDTSFIWFLNPLKSIKYLICNRYKWLIIKIVLALLLLAMVALFLYSMPGYMVKKLLGA

Target ORF information:

RefSeq Version	XM_031901885.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis otoferlin (otof), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901885.1

ORF Insert Sequence:

ATGAACACCA GCATCATGGC CAACGTCAAG AAAGCTCTCA TTGGAGAAAA CAAGGACTTG 
GTGGACCCCT ATGTGCAAGT TGCCTTTGCA GGGCAGAAGG GAAAGACCTC GGTGCAGAAG 
AGCAGCTACG AGCCCATGTG GAACGAGCAG ATCATTTTCA CCGAGATGTT CCCCCCCCTG 
TGCAAGAGGA TGAAAGTGCA GATCAGAGAT TCCGACAAGG TCAACGATGT CGCCATTGGG 
ACCCACTTCA TTGACCTGAG GAAAATTGCC AACGAGGGGG ACAAGGGATT CCTCCCTACT 
TTCGGCCCAG CCTGGGTGAA TATGTATGGC TCCACCCGTA ACTACACCCT CATGGATGAG 
CACCAGGATC TGAATGAGGG GCTCGGAGAA GGCGTCTCTT TCCGCGCAAG GCTGCTGCTG 
AGTCTCGCTG TTGAGATACT GGATACAACC AACCCAGAGC TCACCAGTTC CACAGAGGTT 
CAAGTTGAAG GAGCTCAACC AGTGGCAGAA ACCTGTACCG GTAAAATGGA AGAGTTCTTC 
CTGTTTGGAG CATTCCTGGA ATCCACCATG ATTGATCGGA AAATTGGGGA CAAGCCAATT 
AACTTTGAGG TCACCATAGG TAACTATGGC AACCAAATAG ATGGAATTTC CAAGCCGAGT 
ATGAGGAAGA GGAAGGAAGG AGGTGACGGA GTCGAGGAGG AGACGGAGCT CTTGCAGAAT 
TCCAGTGATG ATGAGGCTGA TGAAGATGGA GAAATGTTAT CAATCTCTTC GTCCCCTGCA 
ATGAAGCCAC TCATAACTGA TAGGAACTAC TTCCACCTCC CGTTCTTTGA TAAGAAGCCC 
TGTACCTACA TTAAGAGTTG GTGGCAGGAC CAGCGGAGGC GGCTGTACAA TGCCAACATT 
ATGGACAAGA TCGCAGATAA GCTGGAGGAA GGCTTGAACG ATGTTCATGA GATGATAAAG 
ACGGAGAAGC CCTACCCTGA ACGGCGGCTG CGAGGAGTCC TGGAGGAGCT GAGTAGCAGC 
TGTTTTCGAT TTGTTACCTT GGCCAACAAG GACCAGAACC AGAATGGGCA GACGAAACTG 
GACAGGGAGA GGCTGAAGTC CTGCATGAGG GAGCTGGAGA ACATGGGGAC ACAAGCCAAG 
ACCATGAGGT CCCAAGTGAA GAAGAACACG GTTAAGGACA AGCTGAAACT GGTGCAGAAT 
TTCCTGCAGA AGCTCCGGTT CCTGGCAGAT GAGCCCCAGC ACAGCATTCC TGACATCTTC 
ATCTGGATGA TGTCCAACAA CAAGCGCATC GCCTACGCTC GAATCCCTTC CAAAGATATC 
CTGTACTCCA TTGTGGATGA GGAGACGGGA AAGGACTGTG GCAAAGTCAA GTGTGTCTTC 
CTTAAGCTGC CGGGCAAGAG GGGCTTTGGG CCTGCAGGCT GGACGGTACA AGCCAAGATG 
GAGGTCTACA TGTGGTTGGG CCTTAGCAAG CAGCGCAGGG ATTTCTTGTG TGGTCTTCCA 
TGTGGATTTG AGGAGATAAA AGCAGTGAAA GGGCCCGGTC TGCAGTCCTT CCCACCCATC 
AGCCTCAGCT ACACCAAAAA ACAAGTGTTC CAGTTACGAG CCCACATGTA CCAAGCCCGG 
AGTCTCTTTG CCGCTGACAG CAGTGGACTT TCCGACCCCT TTGCCCGCGT CTTCTTCAAC 
ACTCAGAGCC AATGTACAGA GGTGCTGAAT GAGACCCTTT GTCCGACTTG GGACCAGCTG 
TTGGTGTTTG ACAATATCGA ACTCTACGGA GAAGCTCACG AGATGAGAGA CGACCCCCCA 
ATCATTGTCA TTGAGATTTA TGATCAAGAC ACAGTAGGGA AGGCCGATTT CATGGGGAGA 
ACATTTGCCA AACCTGTTGT GAAAATGTCA GATGAGCGGT ACTGCCCTCC CCGCTTTCCG 
CCACAGCTGG AATACTACCA GATCTACCGT GGGAACTCAA CCGCAGGGGA CCTCTTGGGA 
GCTTTCGAAC TTCTCCAGAT CGGACCAGCG GGCAAGTCTG ACCTCCCACA GATTGAAGGA 
CCAACAGACA TAGAGAGAGG ACCCATCCTT CCAGTTCCTC TTGGAATCAG ACCAGTGCTC 
AGCAAATACA GAGTAGAGGT CCTTTTCTGG GGGCTGAGAG ACCTGAAGAG AGTAAATCTA 
GCCCAGGTTG ATAGACCAAG GGTGGATATT GAGTGTGCCG GGAGGGGGGT GCAGTCTGCC 
CTCATCCAGA ACTACAAGAA GAATCCAAAT TTTAGCACGT TGGTCAAATG GTTTGAAGTG 
GATCTCCCCG AGAATGAGTT ACTTCACCCG CCATTGAATA TCCGAGTAGT AGACTGCCGA 
GCCTTTGGCA GATACACTCT GGTTGGTTCC CATGCTGTCA GCTCCCTACG CAGGTTCATT 
TACCGGCCTC CGGATAAGAA CTCCCAGCAG TGGACCACAG CAGGTGAAAT AGTTATCAAC 
ATGGAGCCCG AAGCGCCCGT CAAGAAGATG GAGACAATGG TGAAACTGGA AGCCAATTCC 
GACGCGGTGG TTAAAGTAGA CGTGAGCGAG GAAGAAAAAG ACAAGAAGAA GAAGAAGAAG 
AAAGGAGTAG AGGAGATAGA GGAAGAGGAG CCAGATGAGA GCATGTTGGA CTGGTGGTCC 
AAATACTTTG CTTCCATTGA AACCATGAAG GAGCAACTGC GCCAACAGGA AGCGGCAGCC 
GCCGAAGCCG AAGAGAAGGA AGAGATGGAG ATCGCAGAGG GCAACAAGGG CCCCACGAAG 
AACAAGGAGA AGGTCAAAGC CCCAAAAGAG GAGAAGAAGA ACAAGAAGCA GCAACAGCAG 
CAGCAACAAG CCGATCAGGC CGAGAAGAAG AACAAGCAAA AGATCGATGA GTTAAAGGTG 
TACAACAAGG AGTTGGAGAC CGAGTATGAC AACTTTGAGG ACTGGCTCCA TACATTTAAC 
TTGTTACGAG GGAAGATTGG AGATGATGAC GATACTGTAA GCGAAGAAGA GCGAATTGTT 
GGGAGGTTTA AGGGTTCCAT GTGTGTGTAC AAAGTTCCTT TGCCAGAAGA CGTCACAAAG 
GAAGCCGGGT ATGACCCAAA CTTCGGCATG TTCCAAGGAA TCCCTAACAA CGACCCAATG 
AATGTGCTGG TCAGAGTTTA TGTCGTGAGG GCCACCGATC TTCATCCAGC TGATATCAAT 
GGTAAAGCGG ATCCATATGT TGTCATAAAA CTAGGAAAAA CTGATATAAA AGATAAGGAA 
AACTACATCT CCAAGCAGCT AAACCCTGTC TTCGGAAAGT CCTTTGATAT TGAGGCCACT 
TTTCCGATGG AGTCCATGCT TACAGTTGCG GTTTATGACT GGGATCTGGT GGGGACGGAT 
GATCTGATCG GTGAAACCAA AATCGACCTG GAAAACCGTT ACTACAGCAA GCACAGGGCG 
ACTTGTGGTA TCTCAAAGAC ATATGCCATT CACGGGTACA ACTTGTGGAG GGACCCAGTG 
AAGCCTACAC AAATCTTATC CAAACTTTGC AAAGAAGGGA AGATCGATGG ACCTCATTAT 
GGCCCTGGTG GAAGAGTGAA AGTCACGAAT CGGGTATTCA CTGGTCCCAC AGAAATTGAT 
GATGAAAATG GTCAGAAGAA ACAAACGGAT GAGCACCTTG CACTCTCGGT CCTTCAGCAC 
TGGGAGGACA TTCCTCGGGT GGGATGTAAG CTGGTCCCAG AGCATGTAGA AACGAGGCCT 
TTGTTAAACC CAGACAAACC TGGAATTGAG CAGGGCCGCA TAGAAATGTG GGTAGACATG 
TTCCCCATGG ACATGCCTGC GCCAGGTCCT GCCATTGATA TCTCTCCAAG GAAGCCAAAA 
AGATACGAGC TTAGAGTGAT AATATGGAAT ACAGATGAGG TAATCCTGGA AGATGATGAT 
TACTTCACAG GAGAAAAATC TAGTGACATA TTTGTCAGGG GGTGGCTGAA GGGACAGCAG 
GAAGACAAAC AAGACACGGA TGTCCACTAT CATTCCCTTA CTGGGGAGGG GAACTTCAAC 
TGGAGGTTCT TGTTCCCTTT TGATTACCTT ATGGCAGAGG AGAAGATTGT CATCTCCAAG 
AAAGAGTCCA TGTTCTCTTG GGATGAGACC GAGTACAAGA TCCCGGCTCG TCTCACTCTC 
CAGGTCTGGG ATGCCGACCA CTTCTCAGCA GATGACTTTC TCGGAGCCAT AGAATTAGAC 
TTGAATCGCT TTCCCCGAGG GGCAAAAACA GCGAAACAGT GTTCGATAGA AATGGCGGAA 
CCCGACGTCG ATCTGCCAAT GGTCTCAATC TTCAAACAGA AGAGGGTGAA GGGATGGTGG 
CCTTTCGTTG CCAGGAATGA AAACGATGAG ATGGAGCTGA CTGGTAAAGT GGAGGCGGAG 
CTTCACCTTC TCTCAGCAGA GGAGGCAGAG AAGACCCCGG CAGGGCTTGG GCGCAATGAA 
CCAGATCCTC TTGAAAAACC AAATCGACCC GACACCTCGT TCATCTGGTT CCTCAACCCA 
CTTAAGTCCA TCAAGTATCT CATCTGTAAT CGCTACAAGT GGCTCATCAT TAAGATTGTG 
CTGGCGTTGC TGCTTTTGGC CATGGTGGCC CTCTTCCTCT ACAGCATGCC CGGTTACATG 
GTGAAGAAGC TTCTGGGTGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

otof ( XM_012971322.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826776.1 Xenopus tropicalis otoferlin (otof), mRNA.

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CloneID	OXa30655
Clone ID Related Accession (Same CDS sequence)	XM_012971322.1
Accession Version	XM_012971322.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6063bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	otoferlin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683731.1) and transcript sequence (BC136162.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 360 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGCCCTAA TAGTGCATCT CAGGACTGTC ACCGACCTGC GGGGGAAAGG GGACCGGATC 
GCCAAAGTGA CGTTCAGAGG ACTGTCGTTC TACACGCGGG TGTTGGAGAA CTGTGAGGAT 
GAGGCCCGGT TTGATGAGAC GTTCCGATGG CCGATTGCCA GTAACATCGA TGGGAATGAA 
ATGCTGGAGA TCCAAGTGTT TAACTACAGC AAAGTCTTCA CTAACAGGCT GATCGGGACG 
TTCCGGATGG TTCTGCAGCA GGTGGTGGAG GAGGCCCAGC TGGAAGTGAC AGACACACTC 
ATCGATGACA ATAACTCGGC AATCCGGACG AGCGTCAGCA TTGAGCTCCG GTACCAGACC 
ATGGACGGTT CCGTCGGTGC CTGGAACGAT GGAGAATTTA TGGACGGTAC CAACCTGCAG 
AGCGAGGCAG TTGGGAATTT CCACTTTGAG ACGGAAAGTC TGCTCTCCGG CCAGAGCCAC 
AATTCTGCCG TGTCCCCCGG GAGATCACAG AGCTTGCACT CAGGGGAGAG AGCCTTTAAA 
AGAGTTGGCA AAGGGGTATT TTCAGCCATG AAGCTTGGGA AGACGCGTCC GCCCAAGGAC 
GAGAGCAGAA AGCAAGATGA ACCTGCTGTG CTGGAGACAG AAGACTTGGA CAGGAAAGCA 
ATCAGATTGG CTGGAGGTCT GGACCCCGAC ACCATCTCTC TGGCCTCCGT TACAGCCGTC 
ACCACCAATG TGTCCAATAA AAGGTCCAAA CCTGATATCA AAATGGAGCC AAGTGCTGGG 
CGGCCAATGG ATTACCAAGT GAGCATCACC GTCATAGAGG CCCGACAGCT GGTGGGACTG 
AACATGGACC CGGTGGTGTG TGTGGAGGTG GGGGACGAGA AGAAGTACAC GTCCATGAAG 
GAATCCACTA ACTGCCCCTA CTACAATGAG TACTTTGTCT TCGATTTCCA CGTCCCCCCC 
GACGTGATGT TTGACAAGAT AATCAAGCTG TCGGTGATCC ATTCCAAGAA TCTGCTCCGG 
AGCGGAACCT TAGTGGGCTC CTTTAAGCTG GATGTTGGCA CTGTGTACAC GCAGCCCGAG 
CACCAGTTCT ACCACAAGTG GGCCCTCCTC TCCGACCCCG ACGACATCAC GGCCGGGCTC 
AAAGGCTACC TGAAGTGTGA CATCGCCGTG GTGGGAAAGG GGGACAACAT CAAGACCCCG 
CACAAAGCGA ACGAGACCGA TGAGGATGAT ATCGAGGGGA ACCTGCTCCT CCCGGACGGA 
GTTCCCGCGG AACGTCAGTG GGCTCGATTC TACGTCAAGA TCTACCGAGC CGAAGGCCTG 
CCCAGGATGA ACACCAGCAT CATGGCCAAC GTCAAGAAAG CTCTCATTGG AGAAAACAAG 
GACTTGGTGG ACCCCTATGT GCAAGTTGCC TTTGCAGGGC AGAAGGGAAA GACCTCGGTG 
CAGAAGAGCA GCTACGAGCC CATGTGGAAC GAGCAGATCA TTTTCACCGA GATGTTCCCC 
CCCCTGTGCA AGAGGATGAA AGTGCAGATC AGAGATTCCG ACAAGGTCAA CGATGTCGCC 
ATTGGGACCC ACTTCATTGA CCTGAGGAAA ATTGCCAACG AGGGGGACAA GGGATTCCTC 
CCTACTTTCG GCCCAGCCTG GGTGAATATG TATGGCTCCA CCCGTAACTA CACCCTCATG 
GATGAGCACC AGGATCTGAA TGAGGGGCTC GGAGAAGGCG TCTCTTTCCG CGCAAGGCTG 
CTGCTGAGTC TCGCTGTTGA GATACTGGAT ACAACCAACC CAGAGCTCAC CAGTTCCACA 
GAGGTTCAAA TTGAAGGAGC TCAACCAGTG GCAGAAACCT GTACCGGTAA AATGGAAGAG 
TTCTTCCTGT TTGGAGCATT CCTGGAATCC ACCATGATTG ATCGGAAAAT TGGGGACAAG 
CCAATTAACT TTGAGGTCAC CATAGGTAAC TATGGCAACC AAATAGATGG AATTTCCAAG 
CCGAGTATGA GGAAGAGGAA GGAAGGAGGT GATGGAGTTG AGGAGGAGAC GGAGCTCTTG 
CAGAATTCCA GTGATGATGA GGCTGATGAA GATGGAGAAA TGTTATCAAT CTCTTCGTCC 
CCTGCAATGA AGCCACTCAT AACCGATAGG AACTACTTCC ACCTCCCGTT CTTTGATAAG 
AAGCCCTGTA CCTACATTAA GAGTTGGTGG CAGGACCAGC GGAGGCGGCT CTACAATGCC 
AACATTATGG ACAAGATCGC AGATAAGCTG GAGGAAGGCT TGAACGATGT TCATGAGATG 
ATAAAGACTG AGAAGCCTTA CCCCGAGCGG CGGCTGCGAG GAGTCCTGGA GGAGCTGAGT 
AGCAGCTGTT TTCGATTTGT TACCTTGGCC AACAAGGACC AGAACCAGAA TGGGCAGACG 
AAACTGGACA GGGAGAGGCT GAAGTCCTGC ATGAGGGAGC TGGAGAACAT GGGGACACAA 
GCCAAGACCA TGAGGTCCCA AGTGAAGAAG AACACGGTTA AAGACAAACT GAAACTGGTG 
CAGAATTTCC TACAGAAGCT CCGATTCCTG GCAGATGAGC CCCAGCACAG CATTCCTGAC 
ATCTTCATCT GGATGATGTC CAACAACAAG CGCATCGCCT ACGCTCGAAT CCCTTCCAAA 
GATATCCTGT ACTCCATTGT GGATGAGGAG ACGGGAAAGG ACTGTGGCAA AGTCAAGTGT 
GTCTTCCTTA AGCTGCCGGG CAAGAGGGGC TTTGGGCCTG CAGGCTGGAC GGTACAAGCC 
AAGATGGAGG TCTACATGTG GTTGGGCCTT AGCAAGCAGC GCAGGGATTT CTTGTGTGGT 
CTTCCATGTG GATTTGAGGA GATAAAAGCA GTGAAAGGGC CTGGTCTGCA GTCCTTCCCA 
CCCATCAGCC TTAGCTACAC CAAAAAACAA GTGTTCCAGT TACGAGCCCA CATGTACCAA 
GCCCGGAGTC TCTTTGCCGC TGACAGCAGT GGACTTTCCG ACCCCTTTGC CCGCGTCTTC 
TTCAACACTC AGAGCCAATG TACAGAGGTG CTGAATGAGA CCCTTTGTCC CACTTGGGAC 
CAGCTGTTGG TGTTTGACAA TATCGAACTC TACGGAGAAG CTCACGAGAT GAGAGACGAC 
CCCCCAATCA TTGTCATTGA GATTTATGAT CAAGACACAG TAGGGAAGGC CGATTTCATG 
GGGAGAACAT TTGCCAAACC TGTCGTGAAA ATGTCAGATG AGCGGTACTG CCCTCCCCGC 
TTTCCGCCAC AGCTGGAATA CTACCAGATC TACCGTGGGA ACTCAACCGC AGGGGACCTC 
TTGGGAGCTT TCGAACTTCT CCAGATCGGA CCAGCGGGCA AGTCTGACCT CCCACAGATT 
GAAGGACCAA CAGACATAGA GAGAGGACCC ATCCTTCCAG TTCCTCTTGG AATCAGACCA 
GTGCTCAGCA AATACAGAGT GGAGGTCCTT TTCTGGGGGC TGAGAGACCT GAAGAGAGTA 
AATCTAGCCC AGGTTGATAG ACCAAGGGTG GATATTGAGT GTGCCGGGAG GGGGGTGCAG 
TCTGCCCTCA TCCAGAACTA CAAGAAGAAT CCAAATTTTA GCACGTTGGT CAAATGGTTT 
GAAGTGGATC TCCCCGAGAA CGAGTTACTT CACCCGCCAT TGAATATCCG AGTAGTAGAC 
TGCCGAGCCT TTGGCAGATA CACTCTGGTT GGTTCCCATG CTGTCAGCTC CCTACGCAGG 
TTCATTTACC GGCCTCCGGA TAAGAACTCC CAGCAGTGGA CCACAGCAGC TAAACTCATG 
AATGGCTACT TGGCAATGAC CAATGGAAGG CCCCGCTCTC GCACCACAGG TGAAATAGTT 
ATCAACATGG AGCCTGAAGC GCCTGTCAAG AAGATGGAGA CAATGGTGAA ACTGGAAGCC 
AATTCCGATG CGGTGGTTAA AGTAGACGTG GGCGAGGAAG AAAAAGACAA GAAGAAGAAG 
AAGAAGAAAG GAGTAGAGGA GATAGAGGAA GAGGAGCCAG ATGAGAGCAT GTTGGACTGG 
TGGTCCAAAT ACTTTGCTTC CATTGAAACC ATGAAGGAGC AACTGCGCCA ACAGGAAGCG 
GCAGCCGCCG AAGCCGAAGA GAAGGAAGAG ATGGAGATCG CAGAGGAGAT CAAAACTGAT 
GACTCTCCCA TGAAAGGCAA CAAGGGCCCC ACGAAGAACA AGGAGAAGGT CAAAGCCCCA 
AAAGAGGAGA AGAAGAACAA GAAGCAGCAA CAGCAGCAGC AGCACCAAGC CGATCAGGCC 
GAGAAGAAGA ACAAGCAAAA GATCGATGAG TTAAAGGTGT ACAACAAGGA GTTGGAGACC 
GAGTATGACA ACTTTGAGGA CTGGCTCCAT ACATTTAACT TGTTACGAGG GAAGATTGGA 
GATGATGACG ATACTGTAAG CGAAGAAGAG CGAATTGTTG GGAGGTTTAA GGGTTCCATG 
TGTGTGTACA AAGTTCCTTT GCCAGAAGAC GTCACAAAGG AAGCCGGGTA TGACCCAAAC 
TTCGGCATGT TCCAAGGAAT CCCTAACAAC GACCCAATGA ATGTGCTGGT CAGAGTTTAT 
GTCGTGAGGG CCACCGATCT TCATCCAGCT GATATCAATG GTAAAGCGGA TCCATATGTT 
GTCATAAAAC TAGGAAAAAC TGATATAAAA GATAAGGAAA ACTACATCTC CAAGCAGCTA 
AACCCTGTCT TCGGAAAGTC CTTTGATATT GAGGCCACTT TTCCGATGGA GTCCATGCTT 
ACAGTTGCGG TTTATGACTG GGATCTGGTG GGGACGGATG ATCTGATCGG TGAAACCAAA 
ATCGACCTGG AAAACCGTTA CTACAGCAAG CACAGGGCGA CTTGTGGTAT CTCAAAGACA 
TATGCCATTC ACGGGTACAA CTTGTGGAGG GACCCAGTGA AGCCTACACA AATCTTATCC 
AAACTTTGCA AAGAAGGGAA GATCGATGGA CCTCATTATG GCCCTGGTGG AAGAGTGAAA 
GTCACGAATC GGGTATTCAC TGGTCCCACA GAAATTGATG ATGAAAATGG TCAGAAGAAA 
CAAACGGATG AGCACCTTGC ACTCTCGGTC CTTCAGCACT GGGAGGACAT TCCTCGGGTG 
GGATGTAAGC TGGTCCCAGA GCATGTAGAA ACGAGGCCTT TGTTAAACCC AGACAAACCT 
GGAATTGAGC AGGGCCGCAT AGAAATGTGG GTAGACATGT TCCCCATGGA CATGCCTGCG 
CCAGGTCCTG CCATTGATAT CTCTCCAAGG AAGCCAAAAA GATACGAGCT TAGAGTGATA 
ATATGGAATA CAGATGAGGT AATCCTGGAA GATGATGATT ACTTCACAGG AGAAAAATCT 
AGTGACATAT TTGTCAGGGG GTGGCTGAAG GGACAGCAGG AAGACAAACA AGACACGGAT 
GTCCACTATC ATTCCCTTAC TGGGGAGGGG AACTTCAACT GGAGGTTCTT GTTCCCTTTT 
GATTACCTTA TGGCAGAGGA GAAGATTGTC ATCTCCAAGA AAGAGTCCAT GTTCTCTTGG 
GATGAGACCG AGTACAAGAT CCCGGCTCGT CTCACTCTCC AGGTCTGGGA TGCCGACCAC 
TTCTCAGCAG ATGACTTTCT CGGAGCCATA GAATTAGACT TGAATCGCTT TCCCCGAGGG 
GCAAAAACAG CGAAACAGTG TTCGATAGAA ATGGCGGAAC CCGACGTCGA TCTGCCAATG 
GTCTCAATCT TCAAACAGAA GAGGGTGAAG GGATGGTGGC CTTTCGTTGC CAGGAATGAA 
AACGATGAGA TGGAGCTGAC TGGTAAAGTG GAGGCGGAGC TTCACCTTCT CTCAGCAGAG 
GAGGCAGAGA AGACCCCGGC AGGGCTTGGG CGCAATGAAC CAGATCCTCT TGAAAAACCA 
AATCGACCCG ACACCTCGTT CATCTGGTTC CTCAACCCAC TTAAGTCCAT CAAGTATCTC 
ATCTGTAATC GCTACAAGTG GCTCATCATT AAGATTGTGC TGGCGTTGCT GCTTTTGGCC 
ATGGTGGCCC TCTTCCTCTA CAGCATGCCC GGTTACATGG TGAAGAAGCT TCTGGGTGCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826776.1
CDS	71..6133
Translation	MALIVHLRTVTDLRGKGDRIAKVTFRGLSFYTRVLENCEDEARFDETFRWPIASNIDGNEMLEIQVFNYSKVFTNRLIGTFRMVLQQVVEEAQLEVTDTLIDDNNSAIRTSVSIELRYQTMDGSVGAWNDGEFMDGTNLQSEAVGNFHFETESLLSGQSHNSAVSPGRSQSLHSGERAFKRVGKGVFSAMKLGKTRPPKDESRKQDEPAVLETEDLDRKAIRLAGGLDPDTISLASVTAVTTNVSNKRSKPDIKMEPSAGRPMDYQVSITVIEARQLVGLNMDPVVCVEVGDEKKYTSMKESTNCPYYNEYFVFDFHVPPDVMFDKIIKLSVIHSKNLLRSGTLVGSFKLDVGTVYTQPEHQFYHKWALLSDPDDITAGLKGYLKCDIAVVGKGDNIKTPHKANETDEDDIEGNLLLPDGVPAERQWARFYVKIYRAEGLPRMNTSIMANVKKALIGENKDLVDPYVQVAFAGQKGKTSVQKSSYEPMWNEQIIFTEMFPPLCKRMKVQIRDSDKVNDVAIGTHFIDLRKIANEGDKGFLPTFGPAWVNMYGSTRNYTLMDEHQDLNEGLGEGVSFRARLLLSLAVEILDTTNPELTSSTEVQIEGAQPVAETCTGKMEEFFLFGAFLESTMIDRKIGDKPINFEVTIGNYGNQIDGISKPSMRKRKEGGDGVEEETELLQNSSDDEADEDGEMLSISSSPAMKPLITDRNYFHLPFFDKKPCTYIKSWWQDQRRRLYNANIMDKIADKLEEGLNDVHEMIKTEKPYPERRLRGVLEELSSSCFRFVTLANKDQNQNGQTKLDRERLKSCMRELENMGTQAKTMRSQVKKNTVKDKLKLVQNFLQKLRFLADEPQHSIPDIFIWMMSNNKRIAYARIPSKDILYSIVDEETGKDCGKVKCVFLKLPGKRGFGPAGWTVQAKMEVYMWLGLSKQRRDFLCGLPCGFEEIKAVKGPGLQSFPPISLSYTKKQVFQLRAHMYQARSLFAADSSGLSDPFARVFFNTQSQCTEVLNETLCPTWDQLLVFDNIELYGEAHEMRDDPPIIVIEIYDQDTVGKADFMGRTFAKPVVKMSDERYCPPRFPPQLEYYQIYRGNSTAGDLLGAFELLQIGPAGKSDLPQIEGPTDIERGPILPVPLGIRPVLSKYRVEVLFWGLRDLKRVNLAQVDRPRVDIECAGRGVQSALIQNYKKNPNFSTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHAVSSLRRFIYRPPDKNSQQWTTAAKLMNGYLAMTNGRPRSRTTGEIVINMEPEAPVKKMETMVKLEANSDAVVKVDVGEEEKDKKKKKKKGVEEIEEEEPDESMLDWWSKYFASIETMKEQLRQQEAAAAEAEEKEEMEIAEEIKTDDSPMKGNKGPTKNKEKVKAPKEEKKNKKQQQQQQHQADQAEKKNKQKIDELKVYNKELETEYDNFEDWLHTFNLLRGKIGDDDDTVSEEERIVGRFKGSMCVYKVPLPEDVTKEAGYDPNFGMFQGIPNNDPMNVLVRVYVVRATDLHPADINGKADPYVVIKLGKTDIKDKENYISKQLNPVFGKSFDIEATFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRYYSKHRATCGISKTYAIHGYNLWRDPVKPTQILSKLCKEGKIDGPHYGPGGRVKVTNRVFTGPTEIDDENGQKKQTDEHLALSVLQHWEDIPRVGCKLVPEHVETRPLLNPDKPGIEQGRIEMWVDMFPMDMPAPGPAIDISPRKPKRYELRVIIWNTDEVILEDDDYFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRFLFPFDYLMAEEKIVISKKESMFSWDETEYKIPARLTLQVWDADHFSADDFLGAIELDLNRFPRGAKTAKQCSIEMAEPDVDLPMVSIFKQKRVKGWWPFVARNENDEMELTGKVEAELHLLSAEEAEKTPAGLGRNEPDPLEKPNRPDTSFIWFLNPLKSIKYLICNRYKWLIIKIVLALLLLAMVALFLYSMPGYMVKKLLGA

Target ORF information:

RefSeq Version	XM_012971322.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis otoferlin (otof), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971322.1

ORF Insert Sequence:

ATGGCCCTAA TAGTGCATCT CAGGACTGTC ACCGACCTGC GGGGGAAAGG GGACCGGATC 
GCCAAAGTGA CGTTCAGAGG ACTGTCGTTC TACACGCGGG TGTTGGAGAA CTGTGAGGAT 
GAGGCCCGGT TTGATGAGAC GTTCCGATGG CCGATTGCCA GTAACATCGA TGGGAATGAA 
ATGCTGGAGA TCCAAGTGTT TAACTACAGC AAAGTCTTCA CTAACAGGCT GATCGGGACG 
TTCCGGATGG TTCTGCAGCA GGTGGTGGAG GAGGCCCAGC TGGAAGTGAC AGACACACTC 
ATCGATGACA ATAACTCGGC AATCCGGACG AGCGTCAGCA TTGAGCTCCG GTACCAGACC 
ATGGACGGTT CCGTCGGTGC CTGGAACGAT GGAGAATTTA TGGACGGTAC CAACCTGCAG 
AGCGAGGCAG TTGGGAATTT CCACTTTGAG ACGGAAAGTC TGCTCTCCGG CCAGAGCCAC 
AATTCTGCCG TGTCCCCCGG GAGATCACAG AGCTTGCACT CAGGGGAGAG AGCCTTTAAA 
AGAGTTGGCA AAGGGGTATT TTCAGCCATG AAGCTTGGGA AGACGCGTCC GCCCAAGGAC 
GAGAGCAGAA AGCAAGATGA ACCTGCTGTG CTGGAGACAG AAGACTTGGA CAGGAAAGCA 
ATCAGATTGG CTGGAGGTCT GGACCCCGAC ACCATCTCTC TGGCCTCCGT TACAGCCGTC 
ACCACCAATG TGTCCAATAA AAGGTCCAAA CCTGATATCA AAATGGAGCC AAGTGCTGGG 
CGGCCAATGG ATTACCAAGT GAGCATCACC GTCATAGAGG CCCGACAGCT GGTGGGACTG 
AACATGGACC CGGTGGTGTG TGTGGAGGTG GGGGACGAGA AGAAGTACAC GTCCATGAAG 
GAATCCACTA ACTGCCCCTA CTACAATGAG TACTTTGTCT TCGATTTCCA CGTCCCCCCC 
GACGTGATGT TTGACAAGAT AATCAAGCTG TCGGTGATCC ATTCCAAGAA TCTGCTCCGG 
AGCGGAACCT TAGTGGGCTC CTTTAAGCTG GATGTTGGCA CTGTGTACAC GCAGCCCGAG 
CACCAGTTCT ACCACAAGTG GGCCCTCCTC TCCGACCCCG ACGACATCAC GGCCGGGCTC 
AAAGGCTACC TGAAGTGTGA CATCGCCGTG GTGGGAAAGG GGGACAACAT CAAGACCCCG 
CACAAAGCGA ACGAGACCGA TGAGGATGAT ATCGAGGGGA ACCTGCTCCT CCCGGACGGA 
GTTCCCGCGG AACGTCAGTG GGCTCGATTC TACGTCAAGA TCTACCGAGC CGAAGGCCTG 
CCCAGGATGA ACACCAGCAT CATGGCCAAC GTCAAGAAAG CTCTCATTGG AGAAAACAAG 
GACTTGGTGG ACCCCTATGT GCAAGTTGCC TTTGCAGGGC AGAAGGGAAA GACCTCGGTG 
CAGAAGAGCA GCTACGAGCC CATGTGGAAC GAGCAGATCA TTTTCACCGA GATGTTCCCC 
CCCCTGTGCA AGAGGATGAA AGTGCAGATC AGAGATTCCG ACAAGGTCAA CGATGTCGCC 
ATTGGGACCC ACTTCATTGA CCTGAGGAAA ATTGCCAACG AGGGGGACAA GGGATTCCTC 
CCTACTTTCG GCCCAGCCTG GGTGAATATG TATGGCTCCA CCCGTAACTA CACCCTCATG 
GATGAGCACC AGGATCTGAA TGAGGGGCTC GGAGAAGGCG TCTCTTTCCG CGCAAGGCTG 
CTGCTGAGTC TCGCTGTTGA GATACTGGAT ACAACCAACC CAGAGCTCAC CAGTTCCACA 
GAGGTTCAAA TTGAAGGAGC TCAACCAGTG GCAGAAACCT GTACCGGTAA AATGGAAGAG 
TTCTTCCTGT TTGGAGCATT CCTGGAATCC ACCATGATTG ATCGGAAAAT TGGGGACAAG 
CCAATTAACT TTGAGGTCAC CATAGGTAAC TATGGCAACC AAATAGATGG AATTTCCAAG 
CCGAGTATGA GGAAGAGGAA GGAAGGAGGT GATGGAGTTG AGGAGGAGAC GGAGCTCTTG 
CAGAATTCCA GTGATGATGA GGCTGATGAA GATGGAGAAA TGTTATCAAT CTCTTCGTCC 
CCTGCAATGA AGCCACTCAT AACCGATAGG AACTACTTCC ACCTCCCGTT CTTTGATAAG 
AAGCCCTGTA CCTACATTAA GAGTTGGTGG CAGGACCAGC GGAGGCGGCT CTACAATGCC 
AACATTATGG ACAAGATCGC AGATAAGCTG GAGGAAGGCT TGAACGATGT TCATGAGATG 
ATAAAGACTG AGAAGCCTTA CCCCGAGCGG CGGCTGCGAG GAGTCCTGGA GGAGCTGAGT 
AGCAGCTGTT TTCGATTTGT TACCTTGGCC AACAAGGACC AGAACCAGAA TGGGCAGACG 
AAACTGGACA GGGAGAGGCT GAAGTCCTGC ATGAGGGAGC TGGAGAACAT GGGGACACAA 
GCCAAGACCA TGAGGTCCCA AGTGAAGAAG AACACGGTTA AAGACAAACT GAAACTGGTG 
CAGAATTTCC TACAGAAGCT CCGATTCCTG GCAGATGAGC CCCAGCACAG CATTCCTGAC 
ATCTTCATCT GGATGATGTC CAACAACAAG CGCATCGCCT ACGCTCGAAT CCCTTCCAAA 
GATATCCTGT ACTCCATTGT GGATGAGGAG ACGGGAAAGG ACTGTGGCAA AGTCAAGTGT 
GTCTTCCTTA AGCTGCCGGG CAAGAGGGGC TTTGGGCCTG CAGGCTGGAC GGTACAAGCC 
AAGATGGAGG TCTACATGTG GTTGGGCCTT AGCAAGCAGC GCAGGGATTT CTTGTGTGGT 
CTTCCATGTG GATTTGAGGA GATAAAAGCA GTGAAAGGGC CTGGTCTGCA GTCCTTCCCA 
CCCATCAGCC TTAGCTACAC CAAAAAACAA GTGTTCCAGT TACGAGCCCA CATGTACCAA 
GCCCGGAGTC TCTTTGCCGC TGACAGCAGT GGACTTTCCG ACCCCTTTGC CCGCGTCTTC 
TTCAACACTC AGAGCCAATG TACAGAGGTG CTGAATGAGA CCCTTTGTCC CACTTGGGAC 
CAGCTGTTGG TGTTTGACAA TATCGAACTC TACGGAGAAG CTCACGAGAT GAGAGACGAC 
CCCCCAATCA TTGTCATTGA GATTTATGAT CAAGACACAG TAGGGAAGGC CGATTTCATG 
GGGAGAACAT TTGCCAAACC TGTCGTGAAA ATGTCAGATG AGCGGTACTG CCCTCCCCGC 
TTTCCGCCAC AGCTGGAATA CTACCAGATC TACCGTGGGA ACTCAACCGC AGGGGACCTC 
TTGGGAGCTT TCGAACTTCT CCAGATCGGA CCAGCGGGCA AGTCTGACCT CCCACAGATT 
GAAGGACCAA CAGACATAGA GAGAGGACCC ATCCTTCCAG TTCCTCTTGG AATCAGACCA 
GTGCTCAGCA AATACAGAGT GGAGGTCCTT TTCTGGGGGC TGAGAGACCT GAAGAGAGTA 
AATCTAGCCC AGGTTGATAG ACCAAGGGTG GATATTGAGT GTGCCGGGAG GGGGGTGCAG 
TCTGCCCTCA TCCAGAACTA CAAGAAGAAT CCAAATTTTA GCACGTTGGT CAAATGGTTT 
GAAGTGGATC TCCCCGAGAA CGAGTTACTT CACCCGCCAT TGAATATCCG AGTAGTAGAC 
TGCCGAGCCT TTGGCAGATA CACTCTGGTT GGTTCCCATG CTGTCAGCTC CCTACGCAGG 
TTCATTTACC GGCCTCCGGA TAAGAACTCC CAGCAGTGGA CCACAGCAGC TAAACTCATG 
AATGGCTACT TGGCAATGAC CAATGGAAGG CCCCGCTCTC GCACCACAGG TGAAATAGTT 
ATCAACATGG AGCCTGAAGC GCCTGTCAAG AAGATGGAGA CAATGGTGAA ACTGGAAGCC 
AATTCCGATG CGGTGGTTAA AGTAGACGTG GGCGAGGAAG AAAAAGACAA GAAGAAGAAG 
AAGAAGAAAG GAGTAGAGGA GATAGAGGAA GAGGAGCCAG ATGAGAGCAT GTTGGACTGG 
TGGTCCAAAT ACTTTGCTTC CATTGAAACC ATGAAGGAGC AACTGCGCCA ACAGGAAGCG 
GCAGCCGCCG AAGCCGAAGA GAAGGAAGAG ATGGAGATCG CAGAGGAGAT CAAAACTGAT 
GACTCTCCCA TGAAAGGCAA CAAGGGCCCC ACGAAGAACA AGGAGAAGGT CAAAGCCCCA 
AAAGAGGAGA AGAAGAACAA GAAGCAGCAA CAGCAGCAGC AGCACCAAGC CGATCAGGCC 
GAGAAGAAGA ACAAGCAAAA GATCGATGAG TTAAAGGTGT ACAACAAGGA GTTGGAGACC 
GAGTATGACA ACTTTGAGGA CTGGCTCCAT ACATTTAACT TGTTACGAGG GAAGATTGGA 
GATGATGACG ATACTGTAAG CGAAGAAGAG CGAATTGTTG GGAGGTTTAA GGGTTCCATG 
TGTGTGTACA AAGTTCCTTT GCCAGAAGAC GTCACAAAGG AAGCCGGGTA TGACCCAAAC 
TTCGGCATGT TCCAAGGAAT CCCTAACAAC GACCCAATGA ATGTGCTGGT CAGAGTTTAT 
GTCGTGAGGG CCACCGATCT TCATCCAGCT GATATCAATG GTAAAGCGGA TCCATATGTT 
GTCATAAAAC TAGGAAAAAC TGATATAAAA GATAAGGAAA ACTACATCTC CAAGCAGCTA 
AACCCTGTCT TCGGAAAGTC CTTTGATATT GAGGCCACTT TTCCGATGGA GTCCATGCTT 
ACAGTTGCGG TTTATGACTG GGATCTGGTG GGGACGGATG ATCTGATCGG TGAAACCAAA 
ATCGACCTGG AAAACCGTTA CTACAGCAAG CACAGGGCGA CTTGTGGTAT CTCAAAGACA 
TATGCCATTC ACGGGTACAA CTTGTGGAGG GACCCAGTGA AGCCTACACA AATCTTATCC 
AAACTTTGCA AAGAAGGGAA GATCGATGGA CCTCATTATG GCCCTGGTGG AAGAGTGAAA 
GTCACGAATC GGGTATTCAC TGGTCCCACA GAAATTGATG ATGAAAATGG TCAGAAGAAA 
CAAACGGATG AGCACCTTGC ACTCTCGGTC CTTCAGCACT GGGAGGACAT TCCTCGGGTG 
GGATGTAAGC TGGTCCCAGA GCATGTAGAA ACGAGGCCTT TGTTAAACCC AGACAAACCT 
GGAATTGAGC AGGGCCGCAT AGAAATGTGG GTAGACATGT TCCCCATGGA CATGCCTGCG 
CCAGGTCCTG CCATTGATAT CTCTCCAAGG AAGCCAAAAA GATACGAGCT TAGAGTGATA 
ATATGGAATA CAGATGAGGT AATCCTGGAA GATGATGATT ACTTCACAGG AGAAAAATCT 
AGTGACATAT TTGTCAGGGG GTGGCTGAAG GGACAGCAGG AAGACAAACA AGACACGGAT 
GTCCACTATC ATTCCCTTAC TGGGGAGGGG AACTTCAACT GGAGGTTCTT GTTCCCTTTT 
GATTACCTTA TGGCAGAGGA GAAGATTGTC ATCTCCAAGA AAGAGTCCAT GTTCTCTTGG 
GATGAGACCG AGTACAAGAT CCCGGCTCGT CTCACTCTCC AGGTCTGGGA TGCCGACCAC 
TTCTCAGCAG ATGACTTTCT CGGAGCCATA GAATTAGACT TGAATCGCTT TCCCCGAGGG 
GCAAAAACAG CGAAACAGTG TTCGATAGAA ATGGCGGAAC CCGACGTCGA TCTGCCAATG 
GTCTCAATCT TCAAACAGAA GAGGGTGAAG GGATGGTGGC CTTTCGTTGC CAGGAATGAA 
AACGATGAGA TGGAGCTGAC TGGTAAAGTG GAGGCGGAGC TTCACCTTCT CTCAGCAGAG 
GAGGCAGAGA AGACCCCGGC AGGGCTTGGG CGCAATGAAC CAGATCCTCT TGAAAAACCA 
AATCGACCCG ACACCTCGTT CATCTGGTTC CTCAACCCAC TTAAGTCCAT CAAGTATCTC 
ATCTGTAATC GCTACAAGTG GCTCATCATT AAGATTGTGC TGGCGTTGCT GCTTTTGGCC 
ATGGTGGCCC TCTTCCTCTA CAGCATGCCC GGTTACATGG TGAAGAAGCT TCTGGGTGCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
Proceedings of the National Academy of Sciences of the United States of America99(26)16899-903(2002 Dec)
Strausberg RL,Feingold EA,Grouse LH,Derge JG,Klausner RD,Collins FS,Wagner L,Shenmen CM,Schuler GD,Altschul SF,Zeeberg B,Buetow KH,Schaefer CF,Bhat NK,Hopkins RF,Jordan H,Moore T,Max SI,Wang J,Hsieh F,Diatchenko L,Marusina K,Farmer AA,Rubin GM,Hong L,Stapleton M,Soares MB,Bonaldo MF,Casavant TL,Scheetz TE,Brownstein MJ,Usdin TB,Toshiyuki S,Carninci P,Prange C,Raha SS,Loquellano NA,Peters GJ,Abramson RD,Mullahy SJ,Bosak SA,McEwan PJ,McKernan KJ,Malek JA,Gunaratne PH,Richards S,Worley KC,Hale S,Garcia AM,Gay LJ,Hulyk SW,Villalon DK,Muzny DM,Sodergren EJ,Lu X,Gibbs RA,Fahey J,Helton E,Ketteman M,Madan A,Rodrigues S,Sanchez A,Whiting M,Madan A,Young AC,Shevchenko Y,Bouffard GG,Blakesley RW,Touchman JW,Green ED,Dickson MC,Rodriguez AC,Grimwood J,Schmutz J,Myers RM,Butterfield YS,Krzywinski MI,Skalska U,Smailus DE,Schnerch A,Schein JE,Jones SJ,Marra MA,

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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otof cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

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