OTOF cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	OTOF
Entrez Gene ID	617299
Full Name	otoferlin
General protein information	Preferred Names otoferlin Names otoferlin
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015473545.2	XP_015329031.1	otoferlin isoform X1
XM_005213063.3	XP_005213120.1	otoferlin isoform X3
XM_015473545.1	XP_015329031.1	otoferlin isoform X1
NM_001144107.1	NP_001137579.1	otoferlin
XM_015473546.1	XP_015329032.1	otoferlin isoform X2
XM_015473546.2	XP_015329032.1	otoferlin isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	OTOF	NP_919224.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000389	XP_310715.5
Canis lupus familiaris (dog)	OTOF	XP_005630285.1
Bos taurus (cattle)	OTOF	NP_001137579.1
Xenopus tropicalis (tropical clawed frog)	otof	XP_004918245.1
Mus musculus (house mouse)	Otof	NP_114081.2
Gallus gallus (chicken)	OTOF	XP_420015.2
Danio rerio (zebrafish)	otof	NP_001025283.1
Pan troglodytes (chimpanzee)	OTOF	XP_003949828.1
Macaca mulatta (Rhesus monkey)	OTOF	XP_002799220.1
Rattus norvegicus (Norway rat)	Otof	NP_001263649.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Marçais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following OTOF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the OTOF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa39419	NM_001144107.1 Latest version!	Bos taurus otoferlin (OTOF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa24250	XM_005213063.3 Latest version!	Bos taurus otoferlin (OTOF), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa91931	XM_015473545.2 Latest version!	Bos taurus otoferlin (OTOF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa91932	XM_015473546.2 Latest version!	Bos taurus otoferlin (OTOF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OBa91931	XM_015473545.1	Bos taurus otoferlin (OTOF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa91932	XM_015473546.1	Bos taurus otoferlin (OTOF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

OTOF ( NM_001144107.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001137579.1 Bos taurus otoferlin (OTOF), mRNA.

Price & Availability↑

CloneID	OBa39419
Clone ID Related Accession (Same CDS sequence)	NM_001144107.1
Accession Version	NM_001144107.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1527436800000
Organism	Bos taurus(cattle)
Product	otoferlin
Comment	Comment: INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from NKLS02000011.1. On Jan 22, 2009 this sequence version replaced XM_869533.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145437, SAMN03145443 [ECO:0000348] ##Evidence-Data-END##

ATGGCCCTGC TCGTCCACCT CAAGACAGTC TCCGAGCTGC GGGGCAAGGG TGACCGGATC 
GCCAAAGTGA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TCCTGGAGAG CTGTGAGGAT 
GTGGCTGACT TCGATGAGAC CTTCCGGTGG CCGGTGGCCA GCAGCATCGA CAGCAATGAG 
ATGCTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT GATCGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGAC CGACACGCTG 
ATGGATGACA ACAATGCAAT CATCAAGACC AGCCTGTGCG TGGAGGTCCG GTATCAGGCC 
ACAGATGGCA CAGTGGGCTC CTGGGATGAC GGGGACTTTC TGGGAGAGGA GTCCCTTCAC 
GAGGAAGAGA AGGACAGCCA GGAGACGGAT GGGCTGCTAC CCGGCTCCCG TCCCAGCTCC 
CGGCCATCAG GCGAGAAGAG TTTCCGGAGA GCCGGGAGAA GCGTGTTCTC CGCCATGAAG 
CTCGGCAAGA ACCGGCCTCA CAAGGAGGAA CCCCAAAGAC AAGATGAACC AGCTGTTCTG 
GAGATGGAGG ACCTTGACCG CCTGGCCATT CGGCTGGGGG ATGGGCTGGA CCCTGACTCC 
GTGTCGCTAG CATCCGTCAC AGCCCTCACC ACCAATGTCT CCAACAAGCG GTCTAAGCCA 
GACATCAAGA TGGAGCCAAG TGCCGGGCGG CCAATGGACT ACCAGGTCAG CGTCACAGTC 
ATCGAGGCTC GGCAGCTGGT GGGCTTGAAC ATGGACCCCG TGGTGTGTGT GGAGGTGGGC 
GATGAGAAGA AGTACACCTC CATGAAGGAG TCCACTAACT GCCCCTACTA TAATGAGTAC 
TTCGTCTTTG ACTTCCACGT CTCTCCTGAT GTCATGTTTG ACAAGATCAT CAAGATCTCG 
GTGATTCACT CCAAGAACCT GCTGCGCAGT GGCACCCTGG TGGGGTCCTT CAAAATGGAC 
GTGGGAACCG TGTACTCCCA GCCAGAGCAC CAGTTCCACC ACAAGTGGGC CGTCCTGTCC 
GACCCTGACG ACATCTCCGC GGGACTGAAG GGCTACGTGA AGTGCGATGT CGCTGTGGTG 
GGCAAGGGCG ACAACATTAA GACTCCCCAC AAGGCCAATG AGACGGATGA GGATGACATT 
GAGGGGAACT TGCTGCTCCC CGAAGGGGTG CCCCCTGAAC GCCAGTGGGC CCGGTTCTAC 
GTGAAGATCT ACCGAGCAGA GGGGCTGCCC CGCATGAACA CGAGCCTCAT GGCCAATGTG 
AAGAAGGCTT TCACTGGCGA GAACAAGGAT CTGGTCGACC CCTATGTGCA AGTCTTCTTT 
GCCGGCCAGA AGGGCAAGAC TTCAGTGCAG AAAAGCAGCT ACGAGCCACT GTGGAATGAG 
CAGGTGGTCT TCACAGACCT GTTTCCCCCA CTCTGCAAGC GCATGAAGGT GCAAATCCGG 
GACTCGGACA AGGTCAATGA CGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT 
TCCAACGATG GAGACAAAGG CTTCCTGCCC ACGCTGGGCC CTGCCTGGGT GAACATGTAC 
GGCTCCACAC GCAATTACAC GCTGCTAGAC GAGCATCAGG ACCTGAACGA GGGCCTGGGG 
GAGGGTGTGT CCTTCCGTGC CCGCCTCATG CTGGGCCTGG CGGTGGAGAT CCTGGACACC 
TCCAACCCTG AGATCACCAG CTCCACTGAG GTGCAGGTGG AGCAGGCCAC GCCCATCTCC 
GAGAGCTGCA CGGGGAAAAT AGAAGAATTC TTTCTATTTG GAGCCTTCCT GGAGGCCTCA 
ATGATCGACC GGAAAAATGG AGACAAGCCC ATCACCTTTG AGGTCACCGT AGGCAACTAC 
GGGAACGAAG TCGATGGGCT GTCACGACCC CATCGGCCTC GGCCTCGGAA GGAGCCTGGG 
GATGAGGAGG AAGTCGATCT GATCCAGAAC TTGAGTGATG AAGAGACTGA CGAGGGTGGG 
GGCCTGGCCT CGGTCTCCTC CACGCCACCC ATGCGGCCCC AGATCACTGA CAGGAACTAC 
TTCCATCTGC CCTACCTGGA GCACAAACCC TGCATCTACA TCAAGAGCTG GTGGCCTGAC 
CAACGCCGTC GCCTGTACAA TGCCAACATC ATGGACCACA TCGCAGACAA GCTGGAAGAA 
GGCCTGAATG ATGTGCAGGA GATGATCAAA ACGGAGAAGT CGTACCCTGA GCGTCGACTT 
CGGGGCGTCC TAGAGGAGCT GAGCTCTGGT TGCTACCGCT TCCTCTCCCT CGCGGACAAG 
GACCAGGGCC ACTCATCCCG CACCAGGCTG GACCGGGAGC GTCTCAAGTC CTGCATGCGG 
GAGCTGGAAA GCATGGGGCA GCAGGCCAAG ACCCTGCGGG CACAAGTGAA GCGGCACACG 
GTGCGCGACA AGCTGAGGCT CTGTCAGAAC TTCCTGCAGA AGCTGCGCTT CCTGGCAGAC 
GAGCCCCAGC ACAGCATCCC TGACGTCTTC ATCTGGATGA TGAGCAACAA CAAGCGCATT 
GCCTACACCC GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTGGAGGA GGAACTGGGC 
AAGGACTGTG CCAAGGTCAA GACACTCTTC CTCAAGCTGC CAGGGAAGCG GGGCTTCGGC 
TCGGCAGGCT GGACAGTGCA GGCCAAGCTG GAGCTCTACC TGTGGCTGGG TCTAAGCAAG 
CAGCGCAAGG ACTTCCTGTG TGGCCTGCCC TGCGGCTTCG AGGAGGTCAA GGCAGCCCAG 
GGCCTGGGCC TACTCTCCTT CCCGCCCATC AGCCTGGTCT ACACTAAGAA GCAGGCCTTC 
CAGCTCCGCG CCCACATGTA CCAGGCTCGC AGCCTCTTTG CTGCTGACAG CACCGGCCTC 
TCAGACCCCT TTGCCCGTGT CTTCTTCATC AACCAGAGTC AGTGCACAGA GGTACTGAAT 
GAGACCCTGT GCCCCACCTG GGACCAGATG CTGGTGTTCG ACAACCTGGA GCTCTACGGT 
GAAGCTCATG AGCTGCGGGA CGATCCCCCC ATCATTGTTA TCGAAATCTA CGACCAGGAC 
ACCATGGGCA AAGCCGACTT CATGGGCCGG ACCTTTGCCA AGCCGCTGGT GAAGATGGCC 
GACGAGGCGT ACTGCCCACC CCGCTTCCCG CCCCAGCTGG AGTACTATCA GATCTACCGG 
GGCAATGCCA CGGCCGGGGA CCTGCTGGCT GCCTTCGAGC TGCTGCAGAT TGGACCAGCA 
GGGAAGGCCG ACCTGCCGCC GATCAACGGC CCAGTGGACA TGGACCGAGG TCCCATCATG 
CCTGTGCCTG TGGGCATCCG ACCCGTGCTT AGCAAGTACC GGGTGGAGGT GCTGTTCTGG 
GGCCTGCGAG ACCTGAAGCG GGTAAACCTG GCCCAGGTGG ACAGGCCGCG GGTGGACATC 
GAGTGCGCGG GGAAGGGGGT GCAGTCCTCC CTGATCCACA ATTACAAGAA GAACCCCAAC 
TTCAGCACCC TGGTCAAGTG GTTTGAAGTG GACCTCCCAG AGAACGAGCT CCTGCACCCG 
CCCTTGAACA TCCGCGTGGT GGACTGCCGG GCCTTCGGCC GCTACACCCT GGTCGGCTCC 
CACGCTGTCA GCTCCCTGCG GCGCTTCATC TACCGACCCC CGGACCGCTC AGCTCCCAAC 
TGGAACACTT CGGGCAGGCT CCTCCAGGGC CGCCGTGGGC TGTACAGTGG GGGCCCCTCC 
TCTCGCCTCA CAGGGGAGGT CGTGGTGAGC ATGGAGCCGG AGGTGCCCAT CAAGAAGCTG 
GAGACCATGG TGAAGCTGGA CGCGACTTCC GATGCCGTCA TCAAGGTGGA TGTGGCTGAG 
GAAGAGAAGG AGAGGAAGAA GAAGAAGAAG AAGGGCAGCT CAGATGAGCC GGAGGAGGAG 
GAGCCAGATG AGAGTATGTT GGACTGGTGG TCCAAGTACT TTGCCTCCAT TGACACCATG 
AAAGAGCAAA TTCGACAACA AGAAATCTCA GGAATTGACC TGGAGGAGAA GGAGGAGATG 
GACAACACTG AAGGCCTGAA GGGGCCGATG AAGGGCAAGG AGAAGGCAAG GGCTGCAAAG 
GAGGAGAAGA AGAAAAGAAC CCAGAGCCTA GGCCCTGGCC AGGGGTCGGA GGCCCCCGAG 
AAGAGGAAAC CCAAGATTGA TGAGCTCAAG GTGTACCCCA AGGAGCTGGA GTCAGAGTTC 
GACAACTTCG AGGACTGGCT GCACACGTTC AACCTGCTTC GGGGGAAGAC GGGGGACGAC 
GAGGATGGCG GGACCGAGGA GGAGCGCATT GTGGGCCGGT TCAAGGGCTC CCTCTGCGTG 
TACAAAGTGC CACTCCCAGA GGACGTGTCC CGGGAAGCCG GCTACGACCC CACCTACGGC 
ATGTTCCAGG GCATCCCCAG CAATGACCCC ATCAACGTGC TGGTCCGGGT CTACGTGGTC 
CGGGCCACGG ACCTGCACCC CGCAGACATC AACGGCAAAG CTGACCCGTA CATCGCCATC 
CGGCTAGGCA AGACTGATAT CCGTGACAAG GAGAACTACA TCTCCAAGCA GCTCAACCCC 
GTCTTTGGGA AGTCCTTTGA CATCGAAGCC TGCTTCCCCA TGGAATCCAT GCTGACGGTG 
GCCGTGTACG ACTGGGATCT GGTAGGCACT GATGACCTCA TTGGGGAAAC CAAGATCGAC 
CTGGAAAACC GCTTCTACAG CAAGCACCGC GCCACCTGCG GCATCGCCCA GACCTACTCC 
GTACACGGCT ACAATATCTG GCGGGACCCT ATGAAGCCCA GCCAGATCCT GACCCGCCTC 
TGCAAGGAGG GCAAAGTGGA CGGCCCCCAC TTCGGGCCCC CCGGGAGAGT GAAGGTGTCC 
AACCGCATCT TCACAGGGCC CTCTGAGATC GAGGACGAGA ATGGCCAGAG GAAGCCCACA 
GAAGAGCACG TGGCACTGCT GGCCCTGAGA CACTGGGAGG ACATCCCCCG CGTGGGCTGT 
CGCCTGGTGC CCGAACATGT GGAGACACGG CCGCTGCTCA ACCCCGACAA GCCGGGCATC 
GAACAGGGCC GCCTGGAGCT GTGGGTGGAC ATGTTTCCCA TGGACATGCC GTCCCCCGGG 
ACACCTCTGG ACATCTCCCC TCGGAAGCCC AAGAAGTACG AGCTGAGGGT CATCGTGTGG 
AACACGGATG AGGTGGTCTT GGAAGATGAC GACTTCTTCA CTGGGGAGAA GTCCAGCGAC 
ATTTTCGTGC GGGGGTGGCT GAAGGGCCAG CAGGAGGACA AGCAGGACAC AGACGTCCAC 
TACCACTCCC TCACGGGCGA GGGCAACTTC AACTGGCGCT ACCTGTTCCC CTTCGACTAC 
CTGGCTGCCG AGGAGAAGAT AGTCATCTCC AAGAAGGAGT CCATGTTCTC CTGGGACGAG 
ACCGAATACA AGATCCCCGC ACGGCTCACC CTGCAGATCT GGGACGCCGA CCACTTCTCT 
GCCGATGACT TCCTGGGGGC CATCGAGCTG GACCTGAATC GCTTCCCTCG GGGCGCGAAA 
ACGGCCAAGC AGTGCACCAT GGAGATGGCC ACAGGGGAGG TGGATGTGCC CCTGGTTTCC 
ATCTTCAAGC AGAAGCGTGT CAAAGGCTGG TGGCCCCTCC TAGCCCGCAA CGAGAATGAC 
GAGTTTGAGC TCACGGGCAA AGTGGAAGCA GAGTTGCATC TACTCACCGC CGAGGAGGCG 
GAAAAGAATC CCGTGGGCCT GGCCCGCAAT GAACCTGATC CCCTAGAGAA ACCCAACCGG 
CCCGACACCG CCTTCGTCTG GTTCCTCAAC CCCCTCAAGT CCATCAAGTA CCTCATCTGC 
ACGCGGTACA AGTGGCTCAT CATCAAGATC GTGTTGGCGC TGCTGGGACT GCTCATGCTG 
GCGCTCTTCC TCTACAGCCT CCCCGGTTAC ATGGTCAAGA AGCTCTTGGG GGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137579.1
CDS	1..5997
Translation	MALLVHLKTVSELRGKGDRIAKVTFRGQSFYSRVLESCEDVADFDETFRWPVASSIDSNEMLEIQIFNYSKVFSNKLIGTFRMVLQKVVEENHVEVTDTLMDDNNAIIKTSLCVEVRYQATDGTVGSWDDGDFLGEESLHEEEKDSQETDGLLPGSRPSSRPSGEKSFRRAGRSVFSAMKLGKNRPHKEEPQRQDEPAVLEMEDLDRLAIRLGDGLDPDSVSLASVTALTTNVSNKRSKPDIKMEPSAGRPMDYQVSVTVIEARQLVGLNMDPVVCVEVGDEKKYTSMKESTNCPYYNEYFVFDFHVSPDVMFDKIIKISVIHSKNLLRSGTLVGSFKMDVGTVYSQPEHQFHHKWAVLSDPDDISAGLKGYVKCDVAVVGKGDNIKTPHKANETDEDDIEGNLLLPEGVPPERQWARFYVKIYRAEGLPRMNTSLMANVKKAFTGENKDLVDPYVQVFFAGQKGKTSVQKSSYEPLWNEQVVFTDLFPPLCKRMKVQIRDSDKVNDVAIGTHFIDLRKISNDGDKGFLPTLGPAWVNMYGSTRNYTLLDEHQDLNEGLGEGVSFRARLMLGLAVEILDTSNPEITSSTEVQVEQATPISESCTGKIEEFFLFGAFLEASMIDRKNGDKPITFEVTVGNYGNEVDGLSRPHRPRPRKEPGDEEEVDLIQNLSDEETDEGGGLASVSSTPPMRPQITDRNYFHLPYLEHKPCIYIKSWWPDQRRRLYNANIMDHIADKLEEGLNDVQEMIKTEKSYPERRLRGVLEELSSGCYRFLSLADKDQGHSSRTRLDRERLKSCMRELESMGQQAKTLRAQVKRHTVRDKLRLCQNFLQKLRFLADEPQHSIPDVFIWMMSNNKRIAYTRVPSKDLLFSIVEEELGKDCAKVKTLFLKLPGKRGFGSAGWTVQAKLELYLWLGLSKQRKDFLCGLPCGFEEVKAAQGLGLLSFPPISLVYTKKQAFQLRAHMYQARSLFAADSTGLSDPFARVFFINQSQCTEVLNETLCPTWDQMLVFDNLELYGEAHELRDDPPIIVIEIYDQDTMGKADFMGRTFAKPLVKMADEAYCPPRFPPQLEYYQIYRGNATAGDLLAAFELLQIGPAGKADLPPINGPVDMDRGPIMPVPVGIRPVLSKYRVEVLFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSSLIHNYKKNPNFSTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHAVSSLRRFIYRPPDRSAPNWNTSGRLLQGRRGLYSGGPSSRLTGEVVVSMEPEVPIKKLETMVKLDATSDAVIKVDVAEEEKERKKKKKKGSSDEPEEEEPDESMLDWWSKYFASIDTMKEQIRQQEISGIDLEEKEEMDNTEGLKGPMKGKEKARAAKEEKKKRTQSLGPGQGSEAPEKRKPKIDELKVYPKELESEFDNFEDWLHTFNLLRGKTGDDEDGGTEEERIVGRFKGSLCVYKVPLPEDVSREAGYDPTYGMFQGIPSNDPINVLVRVYVVRATDLHPADINGKADPYIAIRLGKTDIRDKENYISKQLNPVFGKSFDIEACFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRFYSKHRATCGIAQTYSVHGYNIWRDPMKPSQILTRLCKEGKVDGPHFGPPGRVKVSNRIFTGPSEIEDENGQRKPTEEHVALLALRHWEDIPRVGCRLVPEHVETRPLLNPDKPGIEQGRLELWVDMFPMDMPSPGTPLDISPRKPKKYELRVIVWNTDEVVLEDDDFFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYLFPFDYLAAEEKIVISKKESMFSWDETEYKIPARLTLQIWDADHFSADDFLGAIELDLNRFPRGAKTAKQCTMEMATGEVDVPLVSIFKQKRVKGWWPLLARNENDEFELTGKVEAELHLLTAEEAEKNPVGLARNEPDPLEKPNRPDTAFVWFLNPLKSIKYLICTRYKWLIIKIVLALLGLLMLALFLYSLPGYMVKKLLGA

Target ORF information:

RefSeq Version	NM_001144107.1
Organism	Bos taurus(cattle)
Definition	Bos taurus otoferlin (OTOF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144107.1

ORF Insert Sequence:

ATGGCCCTGC TCGTCCACCT CAAGACAGTC TCCGAGCTGC GGGGCAAGGG TGACCGGATC 
GCCAAAGTGA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TCCTGGAGAG CTGTGAGGAT 
GTGGCTGACT TCGATGAGAC CTTCCGGTGG CCGGTGGCCA GCAGCATCGA CAGCAATGAG 
ATGCTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT GATCGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGAC CGACACGCTG 
ATGGATGACA ACAATGCAAT CATCAAGACC AGCCTGTGCG TGGAGGTCCG GTATCAGGCC 
ACAGATGGCA CAGTGGGCTC CTGGGATGAC GGGGACTTTC TGGGAGAGGA GTCCCTTCAC 
GAGGAAGAGA AGGACAGCCA GGAGACGGAT GGGCTGCTAC CCGGCTCCCG TCCCAGCTCC 
CGGCCATCAG GCGAGAAGAG TTTCCGGAGA GCCGGGAGAA GCGTGTTCTC CGCCATGAAG 
CTCGGCAAGA ACCGGCCTCA CAAGGAGGAA CCCCAAAGAC AAGATGAACC AGCTGTTCTG 
GAGATGGAGG ACCTTGACCG CCTGGCCATT CGGCTGGGGG ATGGGCTGGA CCCTGACTCC 
GTGTCGCTAG CATCCGTCAC AGCCCTCACC ACCAATGTCT CCAACAAGCG GTCTAAGCCA 
GACATCAAGA TGGAGCCAAG TGCCGGGCGG CCAATGGACT ACCAGGTCAG CGTCACAGTC 
ATCGAGGCTC GGCAGCTGGT GGGCTTGAAC ATGGACCCCG TGGTGTGTGT GGAGGTGGGC 
GATGAGAAGA AGTACACCTC CATGAAGGAG TCCACTAACT GCCCCTACTA TAATGAGTAC 
TTCGTCTTTG ACTTCCACGT CTCTCCTGAT GTCATGTTTG ACAAGATCAT CAAGATCTCG 
GTGATTCACT CCAAGAACCT GCTGCGCAGT GGCACCCTGG TGGGGTCCTT CAAAATGGAC 
GTGGGAACCG TGTACTCCCA GCCAGAGCAC CAGTTCCACC ACAAGTGGGC CGTCCTGTCC 
GACCCTGACG ACATCTCCGC GGGACTGAAG GGCTACGTGA AGTGCGATGT CGCTGTGGTG 
GGCAAGGGCG ACAACATTAA GACTCCCCAC AAGGCCAATG AGACGGATGA GGATGACATT 
GAGGGGAACT TGCTGCTCCC CGAAGGGGTG CCCCCTGAAC GCCAGTGGGC CCGGTTCTAC 
GTGAAGATCT ACCGAGCAGA GGGGCTGCCC CGCATGAACA CGAGCCTCAT GGCCAATGTG 
AAGAAGGCTT TCACTGGCGA GAACAAGGAT CTGGTCGACC CCTATGTGCA AGTCTTCTTT 
GCCGGCCAGA AGGGCAAGAC TTCAGTGCAG AAAAGCAGCT ACGAGCCACT GTGGAATGAG 
CAGGTGGTCT TCACAGACCT GTTTCCCCCA CTCTGCAAGC GCATGAAGGT GCAAATCCGG 
GACTCGGACA AGGTCAATGA CGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT 
TCCAACGATG GAGACAAAGG CTTCCTGCCC ACGCTGGGCC CTGCCTGGGT GAACATGTAC 
GGCTCCACAC GCAATTACAC GCTGCTAGAC GAGCATCAGG ACCTGAACGA GGGCCTGGGG 
GAGGGTGTGT CCTTCCGTGC CCGCCTCATG CTGGGCCTGG CGGTGGAGAT CCTGGACACC 
TCCAACCCTG AGATCACCAG CTCCACTGAG GTGCAGGTGG AGCAGGCCAC GCCCATCTCC 
GAGAGCTGCA CGGGGAAAAT AGAAGAATTC TTTCTATTTG GAGCCTTCCT GGAGGCCTCA 
ATGATCGACC GGAAAAATGG AGACAAGCCC ATCACCTTTG AGGTCACCGT AGGCAACTAC 
GGGAACGAAG TCGATGGGCT GTCACGACCC CATCGGCCTC GGCCTCGGAA GGAGCCTGGG 
GATGAGGAGG AAGTCGATCT GATCCAGAAC TTGAGTGATG AAGAGACTGA CGAGGGTGGG 
GGCCTGGCCT CGGTCTCCTC CACGCCACCC ATGCGGCCCC AGATCACTGA CAGGAACTAC 
TTCCATCTGC CCTACCTGGA GCACAAACCC TGCATCTACA TCAAGAGCTG GTGGCCTGAC 
CAACGCCGTC GCCTGTACAA TGCCAACATC ATGGACCACA TCGCAGACAA GCTGGAAGAA 
GGCCTGAATG ATGTGCAGGA GATGATCAAA ACGGAGAAGT CGTACCCTGA GCGTCGACTT 
CGGGGCGTCC TAGAGGAGCT GAGCTCTGGT TGCTACCGCT TCCTCTCCCT CGCGGACAAG 
GACCAGGGCC ACTCATCCCG CACCAGGCTG GACCGGGAGC GTCTCAAGTC CTGCATGCGG 
GAGCTGGAAA GCATGGGGCA GCAGGCCAAG ACCCTGCGGG CACAAGTGAA GCGGCACACG 
GTGCGCGACA AGCTGAGGCT CTGTCAGAAC TTCCTGCAGA AGCTGCGCTT CCTGGCAGAC 
GAGCCCCAGC ACAGCATCCC TGACGTCTTC ATCTGGATGA TGAGCAACAA CAAGCGCATT 
GCCTACACCC GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTGGAGGA GGAACTGGGC 
AAGGACTGTG CCAAGGTCAA GACACTCTTC CTCAAGCTGC CAGGGAAGCG GGGCTTCGGC 
TCGGCAGGCT GGACAGTGCA GGCCAAGCTG GAGCTCTACC TGTGGCTGGG TCTAAGCAAG 
CAGCGCAAGG ACTTCCTGTG TGGCCTGCCC TGCGGCTTCG AGGAGGTCAA GGCAGCCCAG 
GGCCTGGGCC TACTCTCCTT CCCGCCCATC AGCCTGGTCT ACACTAAGAA GCAGGCCTTC 
CAGCTCCGCG CCCACATGTA CCAGGCTCGC AGCCTCTTTG CTGCTGACAG CACCGGCCTC 
TCAGACCCCT TTGCCCGTGT CTTCTTCATC AACCAGAGTC AGTGCACAGA GGTACTGAAT 
GAGACCCTGT GCCCCACCTG GGACCAGATG CTGGTGTTCG ACAACCTGGA GCTCTACGGT 
GAAGCTCATG AGCTGCGGGA CGATCCCCCC ATCATTGTTA TCGAAATCTA CGACCAGGAC 
ACCATGGGCA AAGCCGACTT CATGGGCCGG ACCTTTGCCA AGCCGCTGGT GAAGATGGCC 
GACGAGGCGT ACTGCCCACC CCGCTTCCCG CCCCAGCTGG AGTACTATCA GATCTACCGG 
GGCAATGCCA CGGCCGGGGA CCTGCTGGCT GCCTTCGAGC TGCTGCAGAT TGGACCAGCA 
GGGAAGGCCG ACCTGCCGCC GATCAACGGC CCAGTGGACA TGGACCGAGG TCCCATCATG 
CCTGTGCCTG TGGGCATCCG ACCCGTGCTT AGCAAGTACC GGGTGGAGGT GCTGTTCTGG 
GGCCTGCGAG ACCTGAAGCG GGTAAACCTG GCCCAGGTGG ACAGGCCGCG GGTGGACATC 
GAGTGCGCGG GGAAGGGGGT GCAGTCCTCC CTGATCCACA ATTACAAGAA GAACCCCAAC 
TTCAGCACCC TGGTCAAGTG GTTTGAAGTG GACCTCCCAG AGAACGAGCT CCTGCACCCG 
CCCTTGAACA TCCGCGTGGT GGACTGCCGG GCCTTCGGCC GCTACACCCT GGTCGGCTCC 
CACGCTGTCA GCTCCCTGCG GCGCTTCATC TACCGACCCC CGGACCGCTC AGCTCCCAAC 
TGGAACACTT CGGGCAGGCT CCTCCAGGGC CGCCGTGGGC TGTACAGTGG GGGCCCCTCC 
TCTCGCCTCA CAGGGGAGGT CGTGGTGAGC ATGGAGCCGG AGGTGCCCAT CAAGAAGCTG 
GAGACCATGG TGAAGCTGGA CGCGACTTCC GATGCCGTCA TCAAGGTGGA TGTGGCTGAG 
GAAGAGAAGG AGAGGAAGAA GAAGAAGAAG AAGGGCAGCT CAGATGAGCC GGAGGAGGAG 
GAGCCAGATG AGAGTATGTT GGACTGGTGG TCCAAGTACT TTGCCTCCAT TGACACCATG 
AAAGAGCAAA TTCGACAACA AGAAATCTCA GGAATTGACC TGGAGGAGAA GGAGGAGATG 
GACAACACTG AAGGCCTGAA GGGGCCGATG AAGGGCAAGG AGAAGGCAAG GGCTGCAAAG 
GAGGAGAAGA AGAAAAGAAC CCAGAGCCTA GGCCCTGGCC AGGGGTCGGA GGCCCCCGAG 
AAGAGGAAAC CCAAGATTGA TGAGCTCAAG GTGTACCCCA AGGAGCTGGA GTCAGAGTTC 
GACAACTTCG AGGACTGGCT GCACACGTTC AACCTGCTTC GGGGGAAGAC GGGGGACGAC 
GAGGATGGCG GGACCGAGGA GGAGCGCATT GTGGGCCGGT TCAAGGGCTC CCTCTGCGTG 
TACAAAGTGC CACTCCCAGA GGACGTGTCC CGGGAAGCCG GCTACGACCC CACCTACGGC 
ATGTTCCAGG GCATCCCCAG CAATGACCCC ATCAACGTGC TGGTCCGGGT CTACGTGGTC 
CGGGCCACGG ACCTGCACCC CGCAGACATC AACGGCAAAG CTGACCCGTA CATCGCCATC 
CGGCTAGGCA AGACTGATAT CCGTGACAAG GAGAACTACA TCTCCAAGCA GCTCAACCCC 
GTCTTTGGGA AGTCCTTTGA CATCGAAGCC TGCTTCCCCA TGGAATCCAT GCTGACGGTG 
GCCGTGTACG ACTGGGATCT GGTAGGCACT GATGACCTCA TTGGGGAAAC CAAGATCGAC 
CTGGAAAACC GCTTCTACAG CAAGCACCGC GCCACCTGCG GCATCGCCCA GACCTACTCC 
GTACACGGCT ACAATATCTG GCGGGACCCT ATGAAGCCCA GCCAGATCCT GACCCGCCTC 
TGCAAGGAGG GCAAAGTGGA CGGCCCCCAC TTCGGGCCCC CCGGGAGAGT GAAGGTGTCC 
AACCGCATCT TCACAGGGCC CTCTGAGATC GAGGACGAGA ATGGCCAGAG GAAGCCCACA 
GAAGAGCACG TGGCACTGCT GGCCCTGAGA CACTGGGAGG ACATCCCCCG CGTGGGCTGT 
CGCCTGGTGC CCGAACATGT GGAGACACGG CCGCTGCTCA ACCCCGACAA GCCGGGCATC 
GAACAGGGCC GCCTGGAGCT GTGGGTGGAC ATGTTTCCCA TGGACATGCC GTCCCCCGGG 
ACACCTCTGG ACATCTCCCC TCGGAAGCCC AAGAAGTACG AGCTGAGGGT CATCGTGTGG 
AACACGGATG AGGTGGTCTT GGAAGATGAC GACTTCTTCA CTGGGGAGAA GTCCAGCGAC 
ATTTTCGTGC GGGGGTGGCT GAAGGGCCAG CAGGAGGACA AGCAGGACAC AGACGTCCAC 
TACCACTCCC TCACGGGCGA GGGCAACTTC AACTGGCGCT ACCTGTTCCC CTTCGACTAC 
CTGGCTGCCG AGGAGAAGAT AGTCATCTCC AAGAAGGAGT CCATGTTCTC CTGGGACGAG 
ACCGAATACA AGATCCCCGC ACGGCTCACC CTGCAGATCT GGGACGCCGA CCACTTCTCT 
GCCGATGACT TCCTGGGGGC CATCGAGCTG GACCTGAATC GCTTCCCTCG GGGCGCGAAA 
ACGGCCAAGC AGTGCACCAT GGAGATGGCC ACAGGGGAGG TGGATGTGCC CCTGGTTTCC 
ATCTTCAAGC AGAAGCGTGT CAAAGGCTGG TGGCCCCTCC TAGCCCGCAA CGAGAATGAC 
GAGTTTGAGC TCACGGGCAA AGTGGAAGCA GAGTTGCATC TACTCACCGC CGAGGAGGCG 
GAAAAGAATC CCGTGGGCCT GGCCCGCAAT GAACCTGATC CCCTAGAGAA ACCCAACCGG 
CCCGACACCG CCTTCGTCTG GTTCCTCAAC CCCCTCAAGT CCATCAAGTA CCTCATCTGC 
ACGCGGTACA AGTGGCTCAT CATCAAGATC GTGTTGGCGC TGCTGGGACT GCTCATGCTG 
GCGCTCTTCC TCTACAGCCT CCCCGGTTAC ATGGTCAAGA AGCTCTTGGG GGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOF ( XM_005213063.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005213120.1 Bos taurus otoferlin (OTOF), transcript variant X3, mRNA.

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CloneID	OBa24250
Clone ID Related Accession (Same CDS sequence)	XM_005213063.3
Accession Version	XM_005213063.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	otoferlin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005213063.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTGC TCGTCCACCT CAAGACAGTC TCCGAGCTGC GGGGCAAGGG TGACCGGATC 
GCCAAAGTGA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TCCTGGAGAG CTGTGAGGAT 
GTGGCTGACT TCGATGAGAC CTTCCGGTGG CCGGTGGCCA GCAGCATCGA CAGCAATGAG 
ATGCTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT GATCGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGAC CGACACGCTG 
ATGGATGACA ACAATGCAAT CATCAAGACC AGCCTGTGCG TGGAGGTCCG GTATCAGGCC 
ACAGATGGCA CAGTGGGCTC CTGGGATGAC GGGGACTTTC TGGGAGAGGA GTCCCTTCAC 
GAGGAAGAGA AGGACAGCCA GGAGACGGAT GGGCTGCTAC CCGGCTCCCG TCCCAGCTCC 
CGGCCATCAG GCGAGAAGAG TTTCCGGAGA GCCGGGAGAA GCGTGTTCTC CGCCATGAAG 
CTCGGCAAGA ACCGGCCTCA CAAGGAGGAA CCCCAAAGAC AAGATGAACC AGCTGTTCTG 
GAGATGGAGG ACCTTGACCG CCTGGCCATT CGGCTGGGGG ATGGGCTGGA CCCTGACTCC 
GTGTCGCTAG CATCCGTCAC AGCCCTCACC ACCAATGTCT CCAACAAGCG GTCTAAGCCA 
GACATCAAGA TGGAGCCAAG TGCCGGGCGG CCAATGGACT ACCAGGTCAG CGTCACAGTC 
ATCGAGGCTC GGCAGCTGGT GGGCTTGAAC ATGGACCCCG TGGTGTGTGT GGAGGTGGGC 
GATGAGAAGA AGTACACCTC CATGAAGGAG TCCACTAACT GCCCCTACTA TAATGAGTAC 
TTCGTCTTTG ACTTCCACGT CTCTCCTGAT GTCATGTTTG ACAAGATCAT CAAGATCTCG 
GTGATTCACT CCAAGAACCT GCTGCGCAGT GGCACCCTGG TGGGGTCCTT CAAAATGGAC 
GTGGGAACCG TGTACTCCCA GCCAGAGCAC CAGTTCCACC ACAAGTGGGC CGTCCTGTCC 
GACCCTGACG ACATCTCCGC GGGACTGAAG GGCTACGTGA AGTGCGATGT CGCTGTGGTG 
GGCAAGGGCG ACAACATTAA GACTCCCCAC AAGGCCAATG AGACGGATGA GGATGACATT 
GAGGGGAACT TGCTGCTCCC CGAAGGGGTG CCCCCTGAAC GCCAGTGGGC CCGGTTCTAC 
GTGAAGATCT ACCGAGCAGA GGGGCTGCCC CGCATGAACA CGAGCCTCAT GGCCAATGTG 
AAGAAGGCTT TCACTGGCGA GAACAAGGAT CTGGTCGACC CCTATGTGCA AGTCTTCTTT 
GCCGGCCAGA AGGGCAAGAC TTCAGTGCAG AAAAGCAGCT ACGAGCCACT GTGGAATGAG 
CAGGTGGTCT TCACAGACCT GTTTCCCCCA CTCTGCAAGC GCATGAAGGT GCAAATCCGG 
GACTCGGACA AGGTCAATGA CGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT 
TCCAACGATG GAGACAAAGG CTTCCTGCCC ACGCTGGGCC CTGCCTGGGT GAACATGTAC 
GGCTCCACAC GCAATTACAC GCTGCTAGAC GAGCATCAGG ACCTGAACGA GGGCCTGGGG 
GAGGGTGTGT CCTTCCGTGC CCGCCTCATG CTGGGCCTGG CGGTGGAGAT CCTGGACACC 
TCCAACCCTG AGATCACCAG CTCCACTGAG GTGCAGGTGG AGCAGGCCAC GCCCATCTCC 
GAGAGCTGCA CGGGGAAAAT AGAAGAATTC TTTCTATTTG GAGCCTTCCT GGAGGCCTCA 
ATGATCGACC GGAAAAATGG AGACAAGCCC ATCACCTTTG AGGTCACCGT AGGCAACTAC 
GGGAACGAAG TCGATGGGCT GTCACGACCC CATCGGCCTC GGCCTCGGAA GGAGCCTGGG 
GATGAGGAGG AAGTCGATCT GATCCAGAAC TTGAGTGATG AAGAGACTGA CGAGGGTGGG 
GGCCTGGCCT CGGTCTCCTC CACGCCACCC ATGCGGCCCC AGATCACTGA CAGGAACTAC 
TTCCATCTGC CCTACCTGGA GCACAAACCC TGCATCTACA TCAAGAGCTG GTGGCCTGAC 
CAACGCCGTC GCCTGTACAA TGCCAACATC ATGGACCACA TCGCAGACAA GCTGGAAGAA 
GGCCTGAATG ATGTGCAGGA GATGATCAAA ACGGAGAAGT CGTACCCTGA GCGTCGACTT 
CGGGGCGTCC TAGAGGAGCT GAGCTCTGGT TGCTACCGCT TCCTCTCCCT CGCGGACAAG 
GACCAGGGCC ACTCATCCCG CACCAGGCTG GACCGGGAGC GTCTCAAGTC CTGCATGCGG 
GAGCTGGAAA GCATGGGGCA GCAGGCCAAG ACCCTGCGGG CACAAGTGAA GCGGCACACG 
GTGCGCGACA AGCTGAGGCT CTGTCAGAAC TTCCTGCAGA AGCTGCGCTT CCTGGCAGAC 
GAGCCCCAGC ACAGCATCCC TGACGTCTTC ATCTGGATGA TGAGCAACAA CAAGCGCATT 
GCCTACACCC GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTGGAGGA GGAACTGGGC 
AAGGACTGTG CCAAGGTCAA GACACTCTTC CTCAAGCTGC CAGGGAAGCG GGGCTTCGGC 
TCGGCAGGCT GGACAGTGCA GGCCAAGCTG GAGCTCTACC TGTGGCTGGG TCTAAGCAAG 
CAGCGCAAGG ACTTCCTGTG TGGCCTGCCC TGCGGCTTCG AGGAGGTCAA GGCAGCCCAG 
GGCCTGGGCC TACTCTCCTT CCCGCCCATC AGCCTGGTCT ACACTAAGAA GCAGGCCTTC 
CAGCTCCGCG CCCACATGTA CCAGGCTCGC AGCCTCTTTG CTGCTGACAG CACCGGCCTC 
TCAGACCCCT TTGCCCGTGT CTTCTTCATC AACCAGAGTC AGTGCACAGA GGTACTGAAT 
GAGACCCTGT GCCCCACCTG GGACCAGATG CTGGTGTTCG ACAACCTGGA GCTCTACGGT 
GAAGCTCATG AGCTGCGGGA CGATCCCCCC ATCATTGTTA TCGAAATCTA CGACCAGGAC 
ACCATGGGCA AAGCCGACTT CATGGGCCGG ACCTTTGCCA AGCCGCTGGT GAAGATGGCC 
GACGAGGCGT ACTGCCCACC CCGCTTCCCG CCCCAGCTGG AGTACTATCA GATCTACCGG 
GGCAATGCCA CGGCCGGGGA CCTGCTGGCT GCCTTCGAGC TGCTGCAGAT TGGACCAGCA 
GGGAAGGCCG ACCTGCCGCC GATCAACGGC CCAGTGGACA TGGACCGAGG TCCCATCATG 
CCTGTGCCTG TGGGCATCCG ACCCGTGCTT AGCAAGTACC GGGTGGAGGT GCTGTTCTGG 
GGCCTGCGAG ACCTGAAGCG GGTAAACCTG GCCCAGGTGG ACAGGCCGCG GGTGGACATC 
GAGTGCGCGG GGAAGGGGGT GCAGTCCTCC CTGATCCACA ATTACAAGAA GAACCCCAAC 
TTCAGCACCC TGGTCAAGTG GTTTGAAGTG GACCTCCCAG AGAACGAGCT CCTGCACCCG 
CCCTTGAACA TCCGCGTGGT GGACTGCCGG GCCTTCGGCC GCTACACCCT GGTCGGCTCC 
CACGCTGTCA GCTCCCTGCG GCGCTTCATC TACCGACCCC CGGACCGCTC AGCTCCCAAC 
TGGAACACTT CGGGGGAGGT CGTGGTGAGC ATGGAGCCGG AGGTGCCCAT CAAGAAGCTG 
GAGACCATGG TGAAGCTGGA CGCGACTTCC GATGCCGTCA TCAAGGTGGA TGTGGCTGAG 
GAAGAGAAGG AGAGGAAGAA GAAGAAGAAG AAGGGCAGCT CAGATGAGCC GGAGGAGGAG 
GAGCCAGATG AGAGTATGTT GGACTGGTGG TCCAAGTACT TTGCCTCCAT TGACACCATG 
AAAGAGCAAA TTCGACAACA AGAAATCTCA GGAATTGACC TGGAGGAGAA GGAGGAGATG 
GACAACACTG AAGGCCTGAA GGGGCCGATG AAGGGCAAGG AGAAGGCAAG GGCTGCAAAG 
GAGGAGAAGA AGAAAAGAAC CCAGAGCCTA GGCCCTGGCC AGGGGTCGGA GGCCCCCGAG 
AAGAGGAAAC CCAAGATTGA TGAGCTCAAG GTGTACCCCA AGGAGCTGGA GTCAGAGTTC 
GACAACTTCG AGGACTGGCT GCACACGTTC AACCTGCTTC GGGGGAAGAC GGGGGACGAC 
GAGGATGGCG GGACCGAGGA GGAGCGCATT GTGGGCCGGT TCAAGGGCTC CCTCTGCGTG 
TACAAAGTGC CACTCCCAGA GGACGTGTCC CGGGAAGCCG GCTACGACCC CACCTACGGC 
ATGTTCCAGG GCATCCCCAG CAATGACCCC ATCAACGTGC TGGTCCGGGT CTACGTGGTC 
CGGGCCACGG ACCTGCACCC CGCAGACATC AACGGCAAAG CTGACCCGTA CATCGCCATC 
CGGCTAGGCA AGACTGATAT CCGTGACAAG GAGAACTACA TCTCCAAGCA GCTCAACCCC 
GTCTTTGGGA AGTCCTTTGA CATCGAAGCC TGCTTCCCCA TGGAATCCAT GCTGACGGTG 
GCCGTGTACG ACTGGGATCT GGTAGGCACT GATGACCTCA TTGGGGAAAC CAAGATCGAC 
CTGGAAAACC GCTTCTACAG CAAGCACCGC GCCACCTGCG GCATCGCCCA GACCTACTCC 
GTACACGGCT ACAATATCTG GCGGGACCCT ATGAAGCCCA GCCAGATCCT GACCCGCCTC 
TGCAAGGAGG GCAAAGTGGA CGGCCCCCAC TTCGGGCCCC CCGGGAGAGT GAAGGTGTCC 
AACCGCATCT TCACAGGGCC CTCTGAGATC GAGGACGAGA ATGGCCAGAG GAAGCCCACA 
GAAGAGCACG TGGCACTGCT GGCCCTGAGA CACTGGGAGG ACATCCCCCG CGTGGGCTGT 
CGCCTGGTGC CCGAACATGT GGAGACACGG CCGCTGCTCA ACCCCGACAA GCCGGGCATC 
GAACAGGGCC GCCTGGAGCT GTGGGTGGAC ATGTTTCCCA TGGACATGCC GTCCCCCGGG 
ACACCTCTGG ACATCTCCCC TCGGAAGCCC AAGAAGTACG AGCTGAGGGT CATCGTGTGG 
AACACGGATG AGGTGGTCTT GGAAGATGAC GACTTCTTCA CTGGGGAGAA GTCCAGCGAC 
ATTTTCGTGC GGGGGTGGCT GAAGGGCCAG CAGGAGGACA AGCAGGACAC AGACGTCCAC 
TACCACTCCC TCACGGGCGA GGGCAACTTC AACTGGCGCT ACCTGTTCCC CTTCGACTAC 
CTGGCTGCCG AGGAGAAGAT AGTCATCTCC AAGAAGGAGT CCATGTTCTC CTGGGACGAG 
ACCGAATACA AGATCCCCGC ACGGCTCACC CTGCAGATCT GGGACGCCGA CCACTTCTCT 
GCCGATGACT TCCTGGGGGC CATCGAGCTG GACCTGAATC GCTTCCCTCG GGGCGCGAAA 
ACGGCCAAGC AGTGCACCAT GGAGATGGCC ACAGGGGAGG TGGATGTGCC CCTGGTTTCC 
ATCTTCAAGC AGAAGCGTGT CAAAGGCTGG TGGCCCCTCC TAGCCCGCAA CGAGAATGAC 
GAGTTTGAGC TCACGGGCAA AGTGGAAGCA GAGTTGCATC TACTCACCGC CGAGGAGGCG 
GAAAAGAATC CCGTGGGCCT GGCCCGCAAT GAACCTGATC CCCTAGAGAA ACCCAACCGG 
CCCGACACCG CCTTCGTCTG GTTCCTCAAC CCCCTCAAGT CCATCAAGTA CCTCATCTGC 
ACGCGGTACA AGTGGCTCAT CATCAAGATC GTGTTGGCGC TGCTGGGACT GCTCATGCTG 
GCGCTCTTCC TCTACAGCCT CCCCGGTTAC ATGGTCAAGA AGCTCTTGGG GGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005213120.1
CDS	1..5937
Translation	MALLVHLKTVSELRGKGDRIAKVTFRGQSFYSRVLESCEDVADFDETFRWPVASSIDSNEMLEIQIFNYSKVFSNKLIGTFRMVLQKVVEENHVEVTDTLMDDNNAIIKTSLCVEVRYQATDGTVGSWDDGDFLGEESLHEEEKDSQETDGLLPGSRPSSRPSGEKSFRRAGRSVFSAMKLGKNRPHKEEPQRQDEPAVLEMEDLDRLAIRLGDGLDPDSVSLASVTALTTNVSNKRSKPDIKMEPSAGRPMDYQVSVTVIEARQLVGLNMDPVVCVEVGDEKKYTSMKESTNCPYYNEYFVFDFHVSPDVMFDKIIKISVIHSKNLLRSGTLVGSFKMDVGTVYSQPEHQFHHKWAVLSDPDDISAGLKGYVKCDVAVVGKGDNIKTPHKANETDEDDIEGNLLLPEGVPPERQWARFYVKIYRAEGLPRMNTSLMANVKKAFTGENKDLVDPYVQVFFAGQKGKTSVQKSSYEPLWNEQVVFTDLFPPLCKRMKVQIRDSDKVNDVAIGTHFIDLRKISNDGDKGFLPTLGPAWVNMYGSTRNYTLLDEHQDLNEGLGEGVSFRARLMLGLAVEILDTSNPEITSSTEVQVEQATPISESCTGKIEEFFLFGAFLEASMIDRKNGDKPITFEVTVGNYGNEVDGLSRPHRPRPRKEPGDEEEVDLIQNLSDEETDEGGGLASVSSTPPMRPQITDRNYFHLPYLEHKPCIYIKSWWPDQRRRLYNANIMDHIADKLEEGLNDVQEMIKTEKSYPERRLRGVLEELSSGCYRFLSLADKDQGHSSRTRLDRERLKSCMRELESMGQQAKTLRAQVKRHTVRDKLRLCQNFLQKLRFLADEPQHSIPDVFIWMMSNNKRIAYTRVPSKDLLFSIVEEELGKDCAKVKTLFLKLPGKRGFGSAGWTVQAKLELYLWLGLSKQRKDFLCGLPCGFEEVKAAQGLGLLSFPPISLVYTKKQAFQLRAHMYQARSLFAADSTGLSDPFARVFFINQSQCTEVLNETLCPTWDQMLVFDNLELYGEAHELRDDPPIIVIEIYDQDTMGKADFMGRTFAKPLVKMADEAYCPPRFPPQLEYYQIYRGNATAGDLLAAFELLQIGPAGKADLPPINGPVDMDRGPIMPVPVGIRPVLSKYRVEVLFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSSLIHNYKKNPNFSTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHAVSSLRRFIYRPPDRSAPNWNTSGEVVVSMEPEVPIKKLETMVKLDATSDAVIKVDVAEEEKERKKKKKKGSSDEPEEEEPDESMLDWWSKYFASIDTMKEQIRQQEISGIDLEEKEEMDNTEGLKGPMKGKEKARAAKEEKKKRTQSLGPGQGSEAPEKRKPKIDELKVYPKELESEFDNFEDWLHTFNLLRGKTGDDEDGGTEEERIVGRFKGSLCVYKVPLPEDVSREAGYDPTYGMFQGIPSNDPINVLVRVYVVRATDLHPADINGKADPYIAIRLGKTDIRDKENYISKQLNPVFGKSFDIEACFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRFYSKHRATCGIAQTYSVHGYNIWRDPMKPSQILTRLCKEGKVDGPHFGPPGRVKVSNRIFTGPSEIEDENGQRKPTEEHVALLALRHWEDIPRVGCRLVPEHVETRPLLNPDKPGIEQGRLELWVDMFPMDMPSPGTPLDISPRKPKKYELRVIVWNTDEVVLEDDDFFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYLFPFDYLAAEEKIVISKKESMFSWDETEYKIPARLTLQIWDADHFSADDFLGAIELDLNRFPRGAKTAKQCTMEMATGEVDVPLVSIFKQKRVKGWWPLLARNENDEFELTGKVEAELHLLTAEEAEKNPVGLARNEPDPLEKPNRPDTAFVWFLNPLKSIKYLICTRYKWLIIKIVLALLGLLMLALFLYSLPGYMVKKLLGA

Target ORF information:

RefSeq Version	XM_005213063.3
Organism	Bos taurus(cattle)
Definition	Bos taurus otoferlin (OTOF), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005213063.3

ORF Insert Sequence:

ATGGCCCTGC TCGTCCACCT CAAGACAGTC TCCGAGCTGC GGGGCAAGGG TGACCGGATC 
GCCAAAGTGA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TCCTGGAGAG CTGTGAGGAT 
GTGGCTGACT TCGATGAGAC CTTCCGGTGG CCGGTGGCCA GCAGCATCGA CAGCAATGAG 
ATGCTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT GATCGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGAC CGACACGCTG 
ATGGATGACA ACAATGCAAT CATCAAGACC AGCCTGTGCG TGGAGGTCCG GTATCAGGCC 
ACAGATGGCA CAGTGGGCTC CTGGGATGAC GGGGACTTTC TGGGAGAGGA GTCCCTTCAC 
GAGGAAGAGA AGGACAGCCA GGAGACGGAT GGGCTGCTAC CCGGCTCCCG TCCCAGCTCC 
CGGCCATCAG GCGAGAAGAG TTTCCGGAGA GCCGGGAGAA GCGTGTTCTC CGCCATGAAG 
CTCGGCAAGA ACCGGCCTCA CAAGGAGGAA CCCCAAAGAC AAGATGAACC AGCTGTTCTG 
GAGATGGAGG ACCTTGACCG CCTGGCCATT CGGCTGGGGG ATGGGCTGGA CCCTGACTCC 
GTGTCGCTAG CATCCGTCAC AGCCCTCACC ACCAATGTCT CCAACAAGCG GTCTAAGCCA 
GACATCAAGA TGGAGCCAAG TGCCGGGCGG CCAATGGACT ACCAGGTCAG CGTCACAGTC 
ATCGAGGCTC GGCAGCTGGT GGGCTTGAAC ATGGACCCCG TGGTGTGTGT GGAGGTGGGC 
GATGAGAAGA AGTACACCTC CATGAAGGAG TCCACTAACT GCCCCTACTA TAATGAGTAC 
TTCGTCTTTG ACTTCCACGT CTCTCCTGAT GTCATGTTTG ACAAGATCAT CAAGATCTCG 
GTGATTCACT CCAAGAACCT GCTGCGCAGT GGCACCCTGG TGGGGTCCTT CAAAATGGAC 
GTGGGAACCG TGTACTCCCA GCCAGAGCAC CAGTTCCACC ACAAGTGGGC CGTCCTGTCC 
GACCCTGACG ACATCTCCGC GGGACTGAAG GGCTACGTGA AGTGCGATGT CGCTGTGGTG 
GGCAAGGGCG ACAACATTAA GACTCCCCAC AAGGCCAATG AGACGGATGA GGATGACATT 
GAGGGGAACT TGCTGCTCCC CGAAGGGGTG CCCCCTGAAC GCCAGTGGGC CCGGTTCTAC 
GTGAAGATCT ACCGAGCAGA GGGGCTGCCC CGCATGAACA CGAGCCTCAT GGCCAATGTG 
AAGAAGGCTT TCACTGGCGA GAACAAGGAT CTGGTCGACC CCTATGTGCA AGTCTTCTTT 
GCCGGCCAGA AGGGCAAGAC TTCAGTGCAG AAAAGCAGCT ACGAGCCACT GTGGAATGAG 
CAGGTGGTCT TCACAGACCT GTTTCCCCCA CTCTGCAAGC GCATGAAGGT GCAAATCCGG 
GACTCGGACA AGGTCAATGA CGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT 
TCCAACGATG GAGACAAAGG CTTCCTGCCC ACGCTGGGCC CTGCCTGGGT GAACATGTAC 
GGCTCCACAC GCAATTACAC GCTGCTAGAC GAGCATCAGG ACCTGAACGA GGGCCTGGGG 
GAGGGTGTGT CCTTCCGTGC CCGCCTCATG CTGGGCCTGG CGGTGGAGAT CCTGGACACC 
TCCAACCCTG AGATCACCAG CTCCACTGAG GTGCAGGTGG AGCAGGCCAC GCCCATCTCC 
GAGAGCTGCA CGGGGAAAAT AGAAGAATTC TTTCTATTTG GAGCCTTCCT GGAGGCCTCA 
ATGATCGACC GGAAAAATGG AGACAAGCCC ATCACCTTTG AGGTCACCGT AGGCAACTAC 
GGGAACGAAG TCGATGGGCT GTCACGACCC CATCGGCCTC GGCCTCGGAA GGAGCCTGGG 
GATGAGGAGG AAGTCGATCT GATCCAGAAC TTGAGTGATG AAGAGACTGA CGAGGGTGGG 
GGCCTGGCCT CGGTCTCCTC CACGCCACCC ATGCGGCCCC AGATCACTGA CAGGAACTAC 
TTCCATCTGC CCTACCTGGA GCACAAACCC TGCATCTACA TCAAGAGCTG GTGGCCTGAC 
CAACGCCGTC GCCTGTACAA TGCCAACATC ATGGACCACA TCGCAGACAA GCTGGAAGAA 
GGCCTGAATG ATGTGCAGGA GATGATCAAA ACGGAGAAGT CGTACCCTGA GCGTCGACTT 
CGGGGCGTCC TAGAGGAGCT GAGCTCTGGT TGCTACCGCT TCCTCTCCCT CGCGGACAAG 
GACCAGGGCC ACTCATCCCG CACCAGGCTG GACCGGGAGC GTCTCAAGTC CTGCATGCGG 
GAGCTGGAAA GCATGGGGCA GCAGGCCAAG ACCCTGCGGG CACAAGTGAA GCGGCACACG 
GTGCGCGACA AGCTGAGGCT CTGTCAGAAC TTCCTGCAGA AGCTGCGCTT CCTGGCAGAC 
GAGCCCCAGC ACAGCATCCC TGACGTCTTC ATCTGGATGA TGAGCAACAA CAAGCGCATT 
GCCTACACCC GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTGGAGGA GGAACTGGGC 
AAGGACTGTG CCAAGGTCAA GACACTCTTC CTCAAGCTGC CAGGGAAGCG GGGCTTCGGC 
TCGGCAGGCT GGACAGTGCA GGCCAAGCTG GAGCTCTACC TGTGGCTGGG TCTAAGCAAG 
CAGCGCAAGG ACTTCCTGTG TGGCCTGCCC TGCGGCTTCG AGGAGGTCAA GGCAGCCCAG 
GGCCTGGGCC TACTCTCCTT CCCGCCCATC AGCCTGGTCT ACACTAAGAA GCAGGCCTTC 
CAGCTCCGCG CCCACATGTA CCAGGCTCGC AGCCTCTTTG CTGCTGACAG CACCGGCCTC 
TCAGACCCCT TTGCCCGTGT CTTCTTCATC AACCAGAGTC AGTGCACAGA GGTACTGAAT 
GAGACCCTGT GCCCCACCTG GGACCAGATG CTGGTGTTCG ACAACCTGGA GCTCTACGGT 
GAAGCTCATG AGCTGCGGGA CGATCCCCCC ATCATTGTTA TCGAAATCTA CGACCAGGAC 
ACCATGGGCA AAGCCGACTT CATGGGCCGG ACCTTTGCCA AGCCGCTGGT GAAGATGGCC 
GACGAGGCGT ACTGCCCACC CCGCTTCCCG CCCCAGCTGG AGTACTATCA GATCTACCGG 
GGCAATGCCA CGGCCGGGGA CCTGCTGGCT GCCTTCGAGC TGCTGCAGAT TGGACCAGCA 
GGGAAGGCCG ACCTGCCGCC GATCAACGGC CCAGTGGACA TGGACCGAGG TCCCATCATG 
CCTGTGCCTG TGGGCATCCG ACCCGTGCTT AGCAAGTACC GGGTGGAGGT GCTGTTCTGG 
GGCCTGCGAG ACCTGAAGCG GGTAAACCTG GCCCAGGTGG ACAGGCCGCG GGTGGACATC 
GAGTGCGCGG GGAAGGGGGT GCAGTCCTCC CTGATCCACA ATTACAAGAA GAACCCCAAC 
TTCAGCACCC TGGTCAAGTG GTTTGAAGTG GACCTCCCAG AGAACGAGCT CCTGCACCCG 
CCCTTGAACA TCCGCGTGGT GGACTGCCGG GCCTTCGGCC GCTACACCCT GGTCGGCTCC 
CACGCTGTCA GCTCCCTGCG GCGCTTCATC TACCGACCCC CGGACCGCTC AGCTCCCAAC 
TGGAACACTT CGGGGGAGGT CGTGGTGAGC ATGGAGCCGG AGGTGCCCAT CAAGAAGCTG 
GAGACCATGG TGAAGCTGGA CGCGACTTCC GATGCCGTCA TCAAGGTGGA TGTGGCTGAG 
GAAGAGAAGG AGAGGAAGAA GAAGAAGAAG AAGGGCAGCT CAGATGAGCC GGAGGAGGAG 
GAGCCAGATG AGAGTATGTT GGACTGGTGG TCCAAGTACT TTGCCTCCAT TGACACCATG 
AAAGAGCAAA TTCGACAACA AGAAATCTCA GGAATTGACC TGGAGGAGAA GGAGGAGATG 
GACAACACTG AAGGCCTGAA GGGGCCGATG AAGGGCAAGG AGAAGGCAAG GGCTGCAAAG 
GAGGAGAAGA AGAAAAGAAC CCAGAGCCTA GGCCCTGGCC AGGGGTCGGA GGCCCCCGAG 
AAGAGGAAAC CCAAGATTGA TGAGCTCAAG GTGTACCCCA AGGAGCTGGA GTCAGAGTTC 
GACAACTTCG AGGACTGGCT GCACACGTTC AACCTGCTTC GGGGGAAGAC GGGGGACGAC 
GAGGATGGCG GGACCGAGGA GGAGCGCATT GTGGGCCGGT TCAAGGGCTC CCTCTGCGTG 
TACAAAGTGC CACTCCCAGA GGACGTGTCC CGGGAAGCCG GCTACGACCC CACCTACGGC 
ATGTTCCAGG GCATCCCCAG CAATGACCCC ATCAACGTGC TGGTCCGGGT CTACGTGGTC 
CGGGCCACGG ACCTGCACCC CGCAGACATC AACGGCAAAG CTGACCCGTA CATCGCCATC 
CGGCTAGGCA AGACTGATAT CCGTGACAAG GAGAACTACA TCTCCAAGCA GCTCAACCCC 
GTCTTTGGGA AGTCCTTTGA CATCGAAGCC TGCTTCCCCA TGGAATCCAT GCTGACGGTG 
GCCGTGTACG ACTGGGATCT GGTAGGCACT GATGACCTCA TTGGGGAAAC CAAGATCGAC 
CTGGAAAACC GCTTCTACAG CAAGCACCGC GCCACCTGCG GCATCGCCCA GACCTACTCC 
GTACACGGCT ACAATATCTG GCGGGACCCT ATGAAGCCCA GCCAGATCCT GACCCGCCTC 
TGCAAGGAGG GCAAAGTGGA CGGCCCCCAC TTCGGGCCCC CCGGGAGAGT GAAGGTGTCC 
AACCGCATCT TCACAGGGCC CTCTGAGATC GAGGACGAGA ATGGCCAGAG GAAGCCCACA 
GAAGAGCACG TGGCACTGCT GGCCCTGAGA CACTGGGAGG ACATCCCCCG CGTGGGCTGT 
CGCCTGGTGC CCGAACATGT GGAGACACGG CCGCTGCTCA ACCCCGACAA GCCGGGCATC 
GAACAGGGCC GCCTGGAGCT GTGGGTGGAC ATGTTTCCCA TGGACATGCC GTCCCCCGGG 
ACACCTCTGG ACATCTCCCC TCGGAAGCCC AAGAAGTACG AGCTGAGGGT CATCGTGTGG 
AACACGGATG AGGTGGTCTT GGAAGATGAC GACTTCTTCA CTGGGGAGAA GTCCAGCGAC 
ATTTTCGTGC GGGGGTGGCT GAAGGGCCAG CAGGAGGACA AGCAGGACAC AGACGTCCAC 
TACCACTCCC TCACGGGCGA GGGCAACTTC AACTGGCGCT ACCTGTTCCC CTTCGACTAC 
CTGGCTGCCG AGGAGAAGAT AGTCATCTCC AAGAAGGAGT CCATGTTCTC CTGGGACGAG 
ACCGAATACA AGATCCCCGC ACGGCTCACC CTGCAGATCT GGGACGCCGA CCACTTCTCT 
GCCGATGACT TCCTGGGGGC CATCGAGCTG GACCTGAATC GCTTCCCTCG GGGCGCGAAA 
ACGGCCAAGC AGTGCACCAT GGAGATGGCC ACAGGGGAGG TGGATGTGCC CCTGGTTTCC 
ATCTTCAAGC AGAAGCGTGT CAAAGGCTGG TGGCCCCTCC TAGCCCGCAA CGAGAATGAC 
GAGTTTGAGC TCACGGGCAA AGTGGAAGCA GAGTTGCATC TACTCACCGC CGAGGAGGCG 
GAAAAGAATC CCGTGGGCCT GGCCCGCAAT GAACCTGATC CCCTAGAGAA ACCCAACCGG 
CCCGACACCG CCTTCGTCTG GTTCCTCAAC CCCCTCAAGT CCATCAAGTA CCTCATCTGC 
ACGCGGTACA AGTGGCTCAT CATCAAGATC GTGTTGGCGC TGCTGGGACT GCTCATGCTG 
GCGCTCTTCC TCTACAGCCT CCCCGGTTAC ATGGTCAAGA AGCTCTTGGG GGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOF ( XM_015473545.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329031.1 Bos taurus otoferlin (OTOF), transcript variant X1, mRNA.

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CloneID	OBa91931
Clone ID Related Accession (Same CDS sequence)	XM_015473545.2 , XM_015473545.1
Accession Version	XM_015473545.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6321bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	otoferlin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015473545.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTGC TCGTCCACCT CAAGACAGTC TCCGAGCTGC GGGGCAAGGG TGACCGGATC 
GCCAAAGTGA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TCCTGGAGAG CTGTGAGGAT 
GTGGCTGACT TCGATGAGAC CTTCCGGTGG CCGGTGGCCA GCAGCATCGA CAGCAATGAG 
ATGCTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT GATCGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGAC CGACACGCTG 
ATGGATGACA ACAATGCAAT CATCAAGACC AGCCTGTGCG TGGAGGTCCG GTATCAGGCC 
ACAGATGGCA CAGTGGGCTC CTGGGATGAC GGGGACTTTC TGGGAGAGGA GTCCCTTCAC 
GAGGAAGAGA AGGACAGCCA GGAGACGGAT GGGCTGCTAC CCGGCTCCCG TCCCAGCTCC 
CGGCCATCAG GCGAGAAGAG TTTCCGGAGA GCCGGGAGAA GCGTGTTCTC CGCCATGAAG 
CTCGGCAAGA ACCGGCCTCA CAAGGAGGAA CCCCAAAGAC AAGATGAACC AGCTGTTCTG 
GAGATGGAGG ACCTTGACCG CCTGGCCATT CGGCTGGGGG ATGGGCTGGA CCCTGACTCC 
GTGTCGCTAG CATCCGTCAC AGCCCTCACC ACCAATGTCT CCAACAAGCG GTCTAAGCCA 
GACATCAAGA TGGAGCCAAG TGCCGGGCGG CCAATGGACT ACCAGGTCAG CGTCACAGTC 
ATCGAGGCTC GGCAGCTGGT GGGCTTGAAC ATGGACCCCG TGGTGTGTGT GGAGGTGGGC 
GATGAGAAGA AGTACACCTC CATGAAGGAG TCCACTAACT GCCCCTACTA TAATGAGTAC 
TTCGTCTTTG ACTTCCACGT CTCTCCTGAT GTCATGTTTG ACAAGATCAT CAAGATCTCG 
GTGATTCACT CCAAGAACCT GCTGCGCAGT GGCACCCTGG TGGGGTCCTT CAAAATGGAC 
GTGGGAACCG TGTACTCCCA GCCAGAGCAC CAGTTCCACC ACAAGTGGGC CGTCCTGTCC 
GACCCTGACG ACATCTCCGC GGGACTGAAG GGCTACGTGA AGTGCGATGT CGCTGTGGTG 
GGCAAGGGCG ACAACATTAA GACTCCCCAC AAGGCCAATG AGACGGATGA GGATGACATT 
GAGGGGAACT TGCTGCTCCC CGAAGGGGTG CCCCCTGAAC GCCAGTGGGC CCGGTTCTAC 
GTGAAGATCT ACCGAGCAGA GGGGCTGCCC CGCATGAACA CGAGCCTCAT GGCCAATGTG 
AAGAAGGCTT TCACTGGCGA GAACAAGGAT CTGGTCGACC CCTATGTGCA AGTCTTCTTT 
GCCGGCCAGA AGGGCAAGAC TTCAGTGCAG AAAAGCAGCT ACGAGCCACT GTGGAATGAG 
CAGGTGGTCT TCACAGACCT GTTTCCCCCA CTCTGCAAGC GCATGAAGGT GCAAATCCGG 
GACTCGGACA AGGTCAATGA CGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT 
TCCAACGATG GAGACAAAGG CTTCCTGCCC ACGCTGGGCC CTGCCTGGGT GAACATGTAC 
GGCTCCACAC GCAATTACAC GCTGCTAGAC GAGCATCAGG ACCTGAACGA GGGCCTGGGG 
GAGGGTGTGT CCTTCCGTGC CCGCCTCATG CTGGGCCTGG CGGTGGAGAT CCTGGACACC 
TCCAACCCTG AGATCACCAG CTCCACTGAG GTGCAGGTGG AGCAGGCCAC GCCCATCTCC 
GAGAGCTGCA CGGGGAAAAT AGAAGAATTC TTTCTATTTG GAGCCTTCCT GGAGGCCTCA 
ATGATCGACC GGAAAAATGG AGACAAGCCC ATCACCTTTG AGGTCACCGT AGGCAACTAC 
GGGAACGAAG TCGATGGGCT GTCACGACCC CATCGGCCTC GGCCTCGGAA GGAGCCTGGG 
GATGAGGAGG AAGTCGATCT GATCCAGAAC TTGAGTGATG AAGAGACTGA CGAGGGTGGG 
GGCCTGGCCT CGGTCTCCTC CACGCCACCC ATGCGGCCCC AGATCACTGA CAGGAACTAC 
TTCCATCTGC CCTACCTGGA GCACAAACCC TGCATCTACA TCAAGAGCTG GTGGCCTGAC 
CAACGCCGTC GCCTGTACAA TGCCAACATC ATGGACCACA TCGCAGACAA GCTGGAAGAA 
GGCCTGAATG ATGTGCAGGA GATGATCAAA ACGGAGAAGT CGTACCCTGA GCGTCGACTT 
CGGGGCGTCC TAGAGGAGCT GAGCTCTGGT TGCTACCGCT TCCTCTCCCT CGCGGACAAG 
GACCAGGGCC ACTCATCCCG CACCAGGCTG GACCGGGAGC GTCTCAAGTC CTGCATGCGG 
GAGCTGGAAA GCATGGGGCA GCAGGCCAAG ACCCTGCGGG CACAAGTGAA GCGGCACACG 
GTGCGCGACA AGCTGAGGCT CTGTCAGAAC TTCCTGCAGA AGCTGCGCTT CCTGGCAGAC 
GAGCCCCAGC ACAGCATCCC TGACGTCTTC ATCTGGATGA TGAGCAACAA CAAGCGCATT 
GCCTACACCC GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTGGAGGA GGAACTGGGC 
AAGGACTGTG CCAAGGTCAA GACACTCTTC CTCAAGGTGC CGGAAGGGGA ATGGATTAGC 
AGCGGGTGGA GGGGCTGGGG GCTGGGCTAC TCTCAAGAGG TCATCTTCCT CTTGGAGGGA 
GCAGCATCCA CACCCCTGGG TCTGCCAGGC CTGAGTGGAC AAGATGCTCA GGGAAGAGCT 
AAAGAGAATA GGAAGGGGGA TCCCGCCCTG CCCATCCCCT TCTGTGCTGT GTTTCCCCAC 
TGCACAGCCC AGTGGGATGG CTGCGGTGGG GATGGGCCCC CTCTGACCAC AGCTTCTTCA 
GCTCCCTGCC CCTTCCTCAC CCCGAGGGCT CTGGCAGCTG ACCCTGAGCC CACCCCCCAG 
CTGCCAGGGA AGCGGGGCTT CGGCTCGGCA GGCTGGACAG TGCAGGCCAA GCTGGAGCTC 
TACCTGTGGC TGGGTCTAAG CAAGCAGCGC AAGGACTTCC TGTGTGGCCT GCCCTGCGGC 
TTCGAGGAGG TCAAGGCAGC CCAGGGCCTG GGCCTACTCT CCTTCCCGCC CATCAGCCTG 
GTCTACACTA AGAAGCAGGC CTTCCAGCTC CGCGCCCACA TGTACCAGGC TCGCAGCCTC 
TTTGCTGCTG ACAGCACCGG CCTCTCAGAC CCCTTTGCCC GTGTCTTCTT CATCAACCAG 
AGTCAGTGCA CAGAGGTACT GAATGAGACC CTGTGCCCCA CCTGGGACCA GATGCTGGTG 
TTCGACAACC TGGAGCTCTA CGGTGAAGCT CATGAGCTGC GGGACGATCC CCCCATCATT 
GTTATCGAAA TCTACGACCA GGACACCATG GGCAAAGCCG ACTTCATGGG CCGGACCTTT 
GCCAAGCCGC TGGTGAAGAT GGCCGACGAG GCGTACTGCC CACCCCGCTT CCCGCCCCAG 
CTGGAGTACT ATCAGATCTA CCGGGGCAAT GCCACGGCCG GGGACCTGCT GGCTGCCTTC 
GAGCTGCTGC AGATTGGACC AGCAGGGAAG GCCGACCTGC CGCCGATCAA CGGCCCAGTG 
GACATGGACC GAGGTCCCAT CATGCCTGTG CCTGTGGGCA TCCGACCCGT GCTTAGCAAG 
TACCGGGTGG AGGTGCTGTT CTGGGGCCTG CGAGACCTGA AGCGGGTAAA CCTGGCCCAG 
GTGGACAGGC CGCGGGTGGA CATCGAGTGC GCGGGGAAGG GGGTGCAGTC CTCCCTGATC 
CACAATTACA AGAAGAACCC CAACTTCAGC ACCCTGGTCA AGTGGTTTGA AGTGGACCTC 
CCAGAGAACG AGCTCCTGCA CCCGCCCTTG AACATCCGCG TGGTGGACTG CCGGGCCTTC 
GGCCGCTACA CCCTGGTCGG CTCCCACGCT GTCAGCTCCC TGCGGCGCTT CATCTACCGA 
CCCCCGGACC GCTCAGCTCC CAACTGGAAC ACTTCGGGCA GGCTCCTCCA GGGCCGCCGT 
GGGCTGTACA GTGGGGGCCC CTCCTCTCGC CTCACAGGGG AGGTCGTGGT GAGCATGGAG 
CCGGAGGTGC CCATCAAGAA GCTGGAGACC ATGGTGAAGC TGGACGCGAC TTCCGATGCC 
GTCATCAAGG TGGATGTGGC TGAGGAAGAG AAGGAGAGGA AGAAGAAGAA GAAGAAGGGC 
AGCTCAGATG AGCCGGAGGA GGAGGAGCCA GATGAGAGTA TGTTGGACTG GTGGTCCAAG 
TACTTTGCCT CCATTGACAC CATGAAAGAG CAAATTCGAC AACAAGAAAT CTCAGGAATT 
GACCTGGAGG AGAAGGAGGA GATGGACAAC ACTGAAGGCC TGAAGGGGCC GATGAAGGGC 
AAGGAGAAGG CAAGGGCTGC AAAGGAGGAG AAGAAGAAAA GAACCCAGAG CCTAGGCCCT 
GGCCAGGGGT CGGAGGCCCC CGAGAAGAGG AAACCCAAGA TTGATGAGCT CAAGGTGTAC 
CCCAAGGAGC TGGAGTCAGA GTTCGACAAC TTCGAGGACT GGCTGCACAC GTTCAACCTG 
CTTCGGGGGA AGACGGGGGA CGACGAGGAT GGCGGGACCG AGGAGGAGCG CATTGTGGGC 
CGGTTCAAGG GCTCCCTCTG CGTGTACAAA GTGCCACTCC CAGAGGACGT GTCCCGGGAA 
GCCGGCTACG ACCCCACCTA CGGCATGTTC CAGGGCATCC CCAGCAATGA CCCCATCAAC 
GTGCTGGTCC GGGTCTACGT GGTCCGGGCC ACGGACCTGC ACCCCGCAGA CATCAACGGC 
AAAGCTGACC CGTACATCGC CATCCGGCTA GGCAAGACTG ATATCCGTGA CAAGGAGAAC 
TACATCTCCA AGCAGCTCAA CCCCGTCTTT GGGAAGTCCT TTGACATCGA AGCCTGCTTC 
CCCATGGAAT CCATGCTGAC GGTGGCCGTG TACGACTGGG ATCTGGTAGG CACTGATGAC 
CTCATTGGGG AAACCAAGAT CGACCTGGAA AACCGCTTCT ACAGCAAGCA CCGCGCCACC 
TGCGGCATCG CCCAGACCTA CTCCGTACAC GGCTACAATA TCTGGCGGGA CCCTATGAAG 
CCCAGCCAGA TCCTGACCCG CCTCTGCAAG GAGGGCAAAG TGGACGGCCC CCACTTCGGG 
CCCCCCGGGA GAGTGAAGGT GTCCAACCGC ATCTTCACAG GGCCCTCTGA GATCGAGGAC 
GAGAATGGCC AGAGGAAGCC CACAGAAGAG CACGTGGCAC TGCTGGCCCT GAGACACTGG 
GAGGACATCC CCCGCGTGGG CTGTCGCCTG GTGCCCGAAC ATGTGGAGAC ACGGCCGCTG 
CTCAACCCCG ACAAGCCGGG CATCGAACAG GGCCGCCTGG AGCTGTGGGT GGACATGTTT 
CCCATGGACA TGCCGTCCCC CGGGACACCT CTGGACATCT CCCCTCGGAA GCCCAAGAAG 
TACGAGCTGA GGGTCATCGT GTGGAACACG GATGAGGTGG TCTTGGAAGA TGACGACTTC 
TTCACTGGGG AGAAGTCCAG CGACATTTTC GTGCGGGGGT GGCTGAAGGG CCAGCAGGAG 
GACAAGCAGG ACACAGACGT CCACTACCAC TCCCTCACGG GCGAGGGCAA CTTCAACTGG 
CGCTACCTGT TCCCCTTCGA CTACCTGGCT GCCGAGGAGA AGATAGTCAT CTCCAAGAAG 
GAGTCCATGT TCTCCTGGGA CGAGACCGAA TACAAGATCC CCGCACGGCT CACCCTGCAG 
ATCTGGGACG CCGACCACTT CTCTGCCGAT GACTTCCTGG GGGCCATCGA GCTGGACCTG 
AATCGCTTCC CTCGGGGCGC GAAAACGGCC AAGCAGTGCA CCATGGAGAT GGCCACAGGG 
GAGGTGGATG TGCCCCTGGT TTCCATCTTC AAGCAGAAGC GTGTCAAAGG CTGGTGGCCC 
CTCCTAGCCC GCAACGAGAA TGACGAGTTT GAGCTCACGG GCAAAGTGGA AGCAGAGTTG 
CATCTACTCA CCGCCGAGGA GGCGGAAAAG AATCCCGTGG GCCTGGCCCG CAATGAACCT 
GATCCCCTAG AGAAACCCAA CCGGCCCGAC ACCGCCTTCG TCTGGTTCCT CAACCCCCTC 
AAGTCCATCA AGTACCTCAT CTGCACGCGG TACAAGTGGC TCATCATCAA GATCGTGTTG 
GCGCTGCTGG GACTGCTCAT GCTGGCGCTC TTCCTCTACA GCCTCCCCGG TTACATGGTC 
AAGAAGCTCT TGGGGGCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329031.1
CDS	181..6501
Translation	MALLVHLKTVSELRGKGDRIAKVTFRGQSFYSRVLESCEDVADFDETFRWPVASSIDSNEMLEIQIFNYSKVFSNKLIGTFRMVLQKVVEENHVEVTDTLMDDNNAIIKTSLCVEVRYQATDGTVGSWDDGDFLGEESLHEEEKDSQETDGLLPGSRPSSRPSGEKSFRRAGRSVFSAMKLGKNRPHKEEPQRQDEPAVLEMEDLDRLAIRLGDGLDPDSVSLASVTALTTNVSNKRSKPDIKMEPSAGRPMDYQVSVTVIEARQLVGLNMDPVVCVEVGDEKKYTSMKESTNCPYYNEYFVFDFHVSPDVMFDKIIKISVIHSKNLLRSGTLVGSFKMDVGTVYSQPEHQFHHKWAVLSDPDDISAGLKGYVKCDVAVVGKGDNIKTPHKANETDEDDIEGNLLLPEGVPPERQWARFYVKIYRAEGLPRMNTSLMANVKKAFTGENKDLVDPYVQVFFAGQKGKTSVQKSSYEPLWNEQVVFTDLFPPLCKRMKVQIRDSDKVNDVAIGTHFIDLRKISNDGDKGFLPTLGPAWVNMYGSTRNYTLLDEHQDLNEGLGEGVSFRARLMLGLAVEILDTSNPEITSSTEVQVEQATPISESCTGKIEEFFLFGAFLEASMIDRKNGDKPITFEVTVGNYGNEVDGLSRPHRPRPRKEPGDEEEVDLIQNLSDEETDEGGGLASVSSTPPMRPQITDRNYFHLPYLEHKPCIYIKSWWPDQRRRLYNANIMDHIADKLEEGLNDVQEMIKTEKSYPERRLRGVLEELSSGCYRFLSLADKDQGHSSRTRLDRERLKSCMRELESMGQQAKTLRAQVKRHTVRDKLRLCQNFLQKLRFLADEPQHSIPDVFIWMMSNNKRIAYTRVPSKDLLFSIVEEELGKDCAKVKTLFLKVPEGEWISSGWRGWGLGYSQEVIFLLEGAASTPLGLPGLSGQDAQGRAKENRKGDPALPIPFCAVFPHCTAQWDGCGGDGPPLTTASSAPCPFLTPRALAADPEPTPQLPGKRGFGSAGWTVQAKLELYLWLGLSKQRKDFLCGLPCGFEEVKAAQGLGLLSFPPISLVYTKKQAFQLRAHMYQARSLFAADSTGLSDPFARVFFINQSQCTEVLNETLCPTWDQMLVFDNLELYGEAHELRDDPPIIVIEIYDQDTMGKADFMGRTFAKPLVKMADEAYCPPRFPPQLEYYQIYRGNATAGDLLAAFELLQIGPAGKADLPPINGPVDMDRGPIMPVPVGIRPVLSKYRVEVLFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSSLIHNYKKNPNFSTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHAVSSLRRFIYRPPDRSAPNWNTSGRLLQGRRGLYSGGPSSRLTGEVVVSMEPEVPIKKLETMVKLDATSDAVIKVDVAEEEKERKKKKKKGSSDEPEEEEPDESMLDWWSKYFASIDTMKEQIRQQEISGIDLEEKEEMDNTEGLKGPMKGKEKARAAKEEKKKRTQSLGPGQGSEAPEKRKPKIDELKVYPKELESEFDNFEDWLHTFNLLRGKTGDDEDGGTEEERIVGRFKGSLCVYKVPLPEDVSREAGYDPTYGMFQGIPSNDPINVLVRVYVVRATDLHPADINGKADPYIAIRLGKTDIRDKENYISKQLNPVFGKSFDIEACFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRFYSKHRATCGIAQTYSVHGYNIWRDPMKPSQILTRLCKEGKVDGPHFGPPGRVKVSNRIFTGPSEIEDENGQRKPTEEHVALLALRHWEDIPRVGCRLVPEHVETRPLLNPDKPGIEQGRLELWVDMFPMDMPSPGTPLDISPRKPKKYELRVIVWNTDEVVLEDDDFFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYLFPFDYLAAEEKIVISKKESMFSWDETEYKIPARLTLQIWDADHFSADDFLGAIELDLNRFPRGAKTAKQCTMEMATGEVDVPLVSIFKQKRVKGWWPLLARNENDEFELTGKVEAELHLLTAEEAEKNPVGLARNEPDPLEKPNRPDTAFVWFLNPLKSIKYLICTRYKWLIIKIVLALLGLLMLALFLYSLPGYMVKKLLGA

Target ORF information:

RefSeq Version	XM_015473545.2
Organism	Bos taurus(cattle)
Definition	Bos taurus otoferlin (OTOF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473545.2

ORF Insert Sequence:

ATGGCCCTGC TCGTCCACCT CAAGACAGTC TCCGAGCTGC GGGGCAAGGG TGACCGGATC 
GCCAAAGTGA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TCCTGGAGAG CTGTGAGGAT 
GTGGCTGACT TCGATGAGAC CTTCCGGTGG CCGGTGGCCA GCAGCATCGA CAGCAATGAG 
ATGCTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT GATCGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGAC CGACACGCTG 
ATGGATGACA ACAATGCAAT CATCAAGACC AGCCTGTGCG TGGAGGTCCG GTATCAGGCC 
ACAGATGGCA CAGTGGGCTC CTGGGATGAC GGGGACTTTC TGGGAGAGGA GTCCCTTCAC 
GAGGAAGAGA AGGACAGCCA GGAGACGGAT GGGCTGCTAC CCGGCTCCCG TCCCAGCTCC 
CGGCCATCAG GCGAGAAGAG TTTCCGGAGA GCCGGGAGAA GCGTGTTCTC CGCCATGAAG 
CTCGGCAAGA ACCGGCCTCA CAAGGAGGAA CCCCAAAGAC AAGATGAACC AGCTGTTCTG 
GAGATGGAGG ACCTTGACCG CCTGGCCATT CGGCTGGGGG ATGGGCTGGA CCCTGACTCC 
GTGTCGCTAG CATCCGTCAC AGCCCTCACC ACCAATGTCT CCAACAAGCG GTCTAAGCCA 
GACATCAAGA TGGAGCCAAG TGCCGGGCGG CCAATGGACT ACCAGGTCAG CGTCACAGTC 
ATCGAGGCTC GGCAGCTGGT GGGCTTGAAC ATGGACCCCG TGGTGTGTGT GGAGGTGGGC 
GATGAGAAGA AGTACACCTC CATGAAGGAG TCCACTAACT GCCCCTACTA TAATGAGTAC 
TTCGTCTTTG ACTTCCACGT CTCTCCTGAT GTCATGTTTG ACAAGATCAT CAAGATCTCG 
GTGATTCACT CCAAGAACCT GCTGCGCAGT GGCACCCTGG TGGGGTCCTT CAAAATGGAC 
GTGGGAACCG TGTACTCCCA GCCAGAGCAC CAGTTCCACC ACAAGTGGGC CGTCCTGTCC 
GACCCTGACG ACATCTCCGC GGGACTGAAG GGCTACGTGA AGTGCGATGT CGCTGTGGTG 
GGCAAGGGCG ACAACATTAA GACTCCCCAC AAGGCCAATG AGACGGATGA GGATGACATT 
GAGGGGAACT TGCTGCTCCC CGAAGGGGTG CCCCCTGAAC GCCAGTGGGC CCGGTTCTAC 
GTGAAGATCT ACCGAGCAGA GGGGCTGCCC CGCATGAACA CGAGCCTCAT GGCCAATGTG 
AAGAAGGCTT TCACTGGCGA GAACAAGGAT CTGGTCGACC CCTATGTGCA AGTCTTCTTT 
GCCGGCCAGA AGGGCAAGAC TTCAGTGCAG AAAAGCAGCT ACGAGCCACT GTGGAATGAG 
CAGGTGGTCT TCACAGACCT GTTTCCCCCA CTCTGCAAGC GCATGAAGGT GCAAATCCGG 
GACTCGGACA AGGTCAATGA CGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT 
TCCAACGATG GAGACAAAGG CTTCCTGCCC ACGCTGGGCC CTGCCTGGGT GAACATGTAC 
GGCTCCACAC GCAATTACAC GCTGCTAGAC GAGCATCAGG ACCTGAACGA GGGCCTGGGG 
GAGGGTGTGT CCTTCCGTGC CCGCCTCATG CTGGGCCTGG CGGTGGAGAT CCTGGACACC 
TCCAACCCTG AGATCACCAG CTCCACTGAG GTGCAGGTGG AGCAGGCCAC GCCCATCTCC 
GAGAGCTGCA CGGGGAAAAT AGAAGAATTC TTTCTATTTG GAGCCTTCCT GGAGGCCTCA 
ATGATCGACC GGAAAAATGG AGACAAGCCC ATCACCTTTG AGGTCACCGT AGGCAACTAC 
GGGAACGAAG TCGATGGGCT GTCACGACCC CATCGGCCTC GGCCTCGGAA GGAGCCTGGG 
GATGAGGAGG AAGTCGATCT GATCCAGAAC TTGAGTGATG AAGAGACTGA CGAGGGTGGG 
GGCCTGGCCT CGGTCTCCTC CACGCCACCC ATGCGGCCCC AGATCACTGA CAGGAACTAC 
TTCCATCTGC CCTACCTGGA GCACAAACCC TGCATCTACA TCAAGAGCTG GTGGCCTGAC 
CAACGCCGTC GCCTGTACAA TGCCAACATC ATGGACCACA TCGCAGACAA GCTGGAAGAA 
GGCCTGAATG ATGTGCAGGA GATGATCAAA ACGGAGAAGT CGTACCCTGA GCGTCGACTT 
CGGGGCGTCC TAGAGGAGCT GAGCTCTGGT TGCTACCGCT TCCTCTCCCT CGCGGACAAG 
GACCAGGGCC ACTCATCCCG CACCAGGCTG GACCGGGAGC GTCTCAAGTC CTGCATGCGG 
GAGCTGGAAA GCATGGGGCA GCAGGCCAAG ACCCTGCGGG CACAAGTGAA GCGGCACACG 
GTGCGCGACA AGCTGAGGCT CTGTCAGAAC TTCCTGCAGA AGCTGCGCTT CCTGGCAGAC 
GAGCCCCAGC ACAGCATCCC TGACGTCTTC ATCTGGATGA TGAGCAACAA CAAGCGCATT 
GCCTACACCC GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTGGAGGA GGAACTGGGC 
AAGGACTGTG CCAAGGTCAA GACACTCTTC CTCAAGGTGC CGGAAGGGGA ATGGATTAGC 
AGCGGGTGGA GGGGCTGGGG GCTGGGCTAC TCTCAAGAGG TCATCTTCCT CTTGGAGGGA 
GCAGCATCCA CACCCCTGGG TCTGCCAGGC CTGAGTGGAC AAGATGCTCA GGGAAGAGCT 
AAAGAGAATA GGAAGGGGGA TCCCGCCCTG CCCATCCCCT TCTGTGCTGT GTTTCCCCAC 
TGCACAGCCC AGTGGGATGG CTGCGGTGGG GATGGGCCCC CTCTGACCAC AGCTTCTTCA 
GCTCCCTGCC CCTTCCTCAC CCCGAGGGCT CTGGCAGCTG ACCCTGAGCC CACCCCCCAG 
CTGCCAGGGA AGCGGGGCTT CGGCTCGGCA GGCTGGACAG TGCAGGCCAA GCTGGAGCTC 
TACCTGTGGC TGGGTCTAAG CAAGCAGCGC AAGGACTTCC TGTGTGGCCT GCCCTGCGGC 
TTCGAGGAGG TCAAGGCAGC CCAGGGCCTG GGCCTACTCT CCTTCCCGCC CATCAGCCTG 
GTCTACACTA AGAAGCAGGC CTTCCAGCTC CGCGCCCACA TGTACCAGGC TCGCAGCCTC 
TTTGCTGCTG ACAGCACCGG CCTCTCAGAC CCCTTTGCCC GTGTCTTCTT CATCAACCAG 
AGTCAGTGCA CAGAGGTACT GAATGAGACC CTGTGCCCCA CCTGGGACCA GATGCTGGTG 
TTCGACAACC TGGAGCTCTA CGGTGAAGCT CATGAGCTGC GGGACGATCC CCCCATCATT 
GTTATCGAAA TCTACGACCA GGACACCATG GGCAAAGCCG ACTTCATGGG CCGGACCTTT 
GCCAAGCCGC TGGTGAAGAT GGCCGACGAG GCGTACTGCC CACCCCGCTT CCCGCCCCAG 
CTGGAGTACT ATCAGATCTA CCGGGGCAAT GCCACGGCCG GGGACCTGCT GGCTGCCTTC 
GAGCTGCTGC AGATTGGACC AGCAGGGAAG GCCGACCTGC CGCCGATCAA CGGCCCAGTG 
GACATGGACC GAGGTCCCAT CATGCCTGTG CCTGTGGGCA TCCGACCCGT GCTTAGCAAG 
TACCGGGTGG AGGTGCTGTT CTGGGGCCTG CGAGACCTGA AGCGGGTAAA CCTGGCCCAG 
GTGGACAGGC CGCGGGTGGA CATCGAGTGC GCGGGGAAGG GGGTGCAGTC CTCCCTGATC 
CACAATTACA AGAAGAACCC CAACTTCAGC ACCCTGGTCA AGTGGTTTGA AGTGGACCTC 
CCAGAGAACG AGCTCCTGCA CCCGCCCTTG AACATCCGCG TGGTGGACTG CCGGGCCTTC 
GGCCGCTACA CCCTGGTCGG CTCCCACGCT GTCAGCTCCC TGCGGCGCTT CATCTACCGA 
CCCCCGGACC GCTCAGCTCC CAACTGGAAC ACTTCGGGCA GGCTCCTCCA GGGCCGCCGT 
GGGCTGTACA GTGGGGGCCC CTCCTCTCGC CTCACAGGGG AGGTCGTGGT GAGCATGGAG 
CCGGAGGTGC CCATCAAGAA GCTGGAGACC ATGGTGAAGC TGGACGCGAC TTCCGATGCC 
GTCATCAAGG TGGATGTGGC TGAGGAAGAG AAGGAGAGGA AGAAGAAGAA GAAGAAGGGC 
AGCTCAGATG AGCCGGAGGA GGAGGAGCCA GATGAGAGTA TGTTGGACTG GTGGTCCAAG 
TACTTTGCCT CCATTGACAC CATGAAAGAG CAAATTCGAC AACAAGAAAT CTCAGGAATT 
GACCTGGAGG AGAAGGAGGA GATGGACAAC ACTGAAGGCC TGAAGGGGCC GATGAAGGGC 
AAGGAGAAGG CAAGGGCTGC AAAGGAGGAG AAGAAGAAAA GAACCCAGAG CCTAGGCCCT 
GGCCAGGGGT CGGAGGCCCC CGAGAAGAGG AAACCCAAGA TTGATGAGCT CAAGGTGTAC 
CCCAAGGAGC TGGAGTCAGA GTTCGACAAC TTCGAGGACT GGCTGCACAC GTTCAACCTG 
CTTCGGGGGA AGACGGGGGA CGACGAGGAT GGCGGGACCG AGGAGGAGCG CATTGTGGGC 
CGGTTCAAGG GCTCCCTCTG CGTGTACAAA GTGCCACTCC CAGAGGACGT GTCCCGGGAA 
GCCGGCTACG ACCCCACCTA CGGCATGTTC CAGGGCATCC CCAGCAATGA CCCCATCAAC 
GTGCTGGTCC GGGTCTACGT GGTCCGGGCC ACGGACCTGC ACCCCGCAGA CATCAACGGC 
AAAGCTGACC CGTACATCGC CATCCGGCTA GGCAAGACTG ATATCCGTGA CAAGGAGAAC 
TACATCTCCA AGCAGCTCAA CCCCGTCTTT GGGAAGTCCT TTGACATCGA AGCCTGCTTC 
CCCATGGAAT CCATGCTGAC GGTGGCCGTG TACGACTGGG ATCTGGTAGG CACTGATGAC 
CTCATTGGGG AAACCAAGAT CGACCTGGAA AACCGCTTCT ACAGCAAGCA CCGCGCCACC 
TGCGGCATCG CCCAGACCTA CTCCGTACAC GGCTACAATA TCTGGCGGGA CCCTATGAAG 
CCCAGCCAGA TCCTGACCCG CCTCTGCAAG GAGGGCAAAG TGGACGGCCC CCACTTCGGG 
CCCCCCGGGA GAGTGAAGGT GTCCAACCGC ATCTTCACAG GGCCCTCTGA GATCGAGGAC 
GAGAATGGCC AGAGGAAGCC CACAGAAGAG CACGTGGCAC TGCTGGCCCT GAGACACTGG 
GAGGACATCC CCCGCGTGGG CTGTCGCCTG GTGCCCGAAC ATGTGGAGAC ACGGCCGCTG 
CTCAACCCCG ACAAGCCGGG CATCGAACAG GGCCGCCTGG AGCTGTGGGT GGACATGTTT 
CCCATGGACA TGCCGTCCCC CGGGACACCT CTGGACATCT CCCCTCGGAA GCCCAAGAAG 
TACGAGCTGA GGGTCATCGT GTGGAACACG GATGAGGTGG TCTTGGAAGA TGACGACTTC 
TTCACTGGGG AGAAGTCCAG CGACATTTTC GTGCGGGGGT GGCTGAAGGG CCAGCAGGAG 
GACAAGCAGG ACACAGACGT CCACTACCAC TCCCTCACGG GCGAGGGCAA CTTCAACTGG 
CGCTACCTGT TCCCCTTCGA CTACCTGGCT GCCGAGGAGA AGATAGTCAT CTCCAAGAAG 
GAGTCCATGT TCTCCTGGGA CGAGACCGAA TACAAGATCC CCGCACGGCT CACCCTGCAG 
ATCTGGGACG CCGACCACTT CTCTGCCGAT GACTTCCTGG GGGCCATCGA GCTGGACCTG 
AATCGCTTCC CTCGGGGCGC GAAAACGGCC AAGCAGTGCA CCATGGAGAT GGCCACAGGG 
GAGGTGGATG TGCCCCTGGT TTCCATCTTC AAGCAGAAGC GTGTCAAAGG CTGGTGGCCC 
CTCCTAGCCC GCAACGAGAA TGACGAGTTT GAGCTCACGG GCAAAGTGGA AGCAGAGTTG 
CATCTACTCA CCGCCGAGGA GGCGGAAAAG AATCCCGTGG GCCTGGCCCG CAATGAACCT 
GATCCCCTAG AGAAACCCAA CCGGCCCGAC ACCGCCTTCG TCTGGTTCCT CAACCCCCTC 
AAGTCCATCA AGTACCTCAT CTGCACGCGG TACAAGTGGC TCATCATCAA GATCGTGTTG 
GCGCTGCTGG GACTGCTCAT GCTGGCGCTC TTCCTCTACA GCCTCCCCGG TTACATGGTC 
AAGAAGCTCT TGGGGGCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOF ( XM_015473545.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329031.1 Bos taurus otoferlin (OTOF), transcript variant X1, mRNA.

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CloneID	OBa91931
Clone ID Related Accession (Same CDS sequence)	XM_015473545.2 , XM_015473545.1
Accession Version	XM_015473545.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6321bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	otoferlin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTGC TCGTCCACCT CAAGACAGTC TCCGAGCTGC GGGGCAAGGG TGACCGGATC 
GCCAAAGTGA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TCCTGGAGAG CTGTGAGGAT 
GTGGCTGACT TCGATGAGAC CTTCCGGTGG CCGGTGGCCA GCAGCATCGA CAGCAATGAG 
ATGCTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT GATCGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGAC CGACACGCTG 
ATGGATGACA ACAATGCAAT CATCAAGACC AGCCTGTGCG TGGAGGTCCG GTATCAGGCC 
ACAGATGGCA CAGTGGGCTC CTGGGATGAC GGGGACTTTC TGGGAGAGGA GTCCCTTCAC 
GAGGAAGAGA AGGACAGCCA GGAGACGGAT GGGCTGCTAC CCGGCTCCCG TCCCAGCTCC 
CGGCCATCAG GCGAGAAGAG TTTCCGGAGA GCCGGGAGAA GCGTGTTCTC CGCCATGAAG 
CTCGGCAAGA ACCGGCCTCA CAAGGAGGAA CCCCAAAGAC AAGATGAACC AGCTGTTCTG 
GAGATGGAGG ACCTTGACCG CCTGGCCATT CGGCTGGGGG ATGGGCTGGA CCCTGACTCC 
GTGTCGCTAG CATCCGTCAC AGCCCTCACC ACCAATGTCT CCAACAAGCG GTCTAAGCCA 
GACATCAAGA TGGAGCCAAG TGCCGGGCGG CCAATGGACT ACCAGGTCAG CGTCACAGTC 
ATCGAGGCTC GGCAGCTGGT GGGCTTGAAC ATGGACCCCG TGGTGTGTGT GGAGGTGGGC 
GATGAGAAGA AGTACACCTC CATGAAGGAG TCCACTAACT GCCCCTACTA TAATGAGTAC 
TTCGTCTTTG ACTTCCACGT CTCTCCTGAT GTCATGTTTG ACAAGATCAT CAAGATCTCG 
GTGATTCACT CCAAGAACCT GCTGCGCAGT GGCACCCTGG TGGGGTCCTT CAAAATGGAC 
GTGGGAACCG TGTACTCCCA GCCAGAGCAC CAGTTCCACC ACAAGTGGGC CGTCCTGTCC 
GACCCTGACG ACATCTCCGC GGGACTGAAG GGCTACGTGA AGTGCGATGT CGCTGTGGTG 
GGCAAGGGCG ACAACATTAA GACTCCCCAC AAGGCCAATG AGACGGATGA GGATGACATT 
GAGGGGAACT TGCTGCTCCC CGAAGGGGTG CCCCCTGAAC GCCAGTGGGC CCGGTTCTAC 
GTGAAGATCT ACCGAGCAGA GGGGCTGCCC CGCATGAACA CGAGCCTCAT GGCCAATGTG 
AAGAAGGCTT TCACTGGCGA GAACAAGGAT CTGGTCGACC CCTATGTGCA AGTCTTCTTT 
GCCGGCCAGA AGGGCAAGAC TTCAGTGCAG AAAAGCAGCT ACGAGCCACT GTGGAATGAG 
CAGGTGGTCT TCACAGACCT GTTTCCCCCA CTCTGCAAGC GCATGAAGGT GCAAATCCGG 
GACTCGGACA AGGTCAATGA CGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT 
TCCAACGATG GAGACAAAGG CTTCCTGCCC ACGCTGGGCC CTGCCTGGGT GAACATGTAC 
GGCTCCACAC GCAATTACAC GCTGCTAGAC GAGCATCAGG ACCTGAACGA GGGCCTGGGG 
GAGGGTGTGT CCTTCCGTGC CCGCCTCATG CTGGGCCTGG CGGTGGAGAT CCTGGACACC 
TCCAACCCTG AGATCACCAG CTCCACTGAG GTGCAGGTGG AGCAGGCCAC GCCCATCTCC 
GAGAGCTGCA CGGGGAAAAT AGAAGAATTC TTTCTATTTG GAGCCTTCCT GGAGGCCTCA 
ATGATCGACC GGAAAAATGG AGACAAGCCC ATCACCTTTG AGGTCACCGT AGGCAACTAC 
GGGAACGAAG TCGATGGGCT GTCACGACCC CATCGGCCTC GGCCTCGGAA GGAGCCTGGG 
GATGAGGAGG AAGTCGATCT GATCCAGAAC TTGAGTGATG AAGAGACTGA CGAGGGTGGG 
GGCCTGGCCT CGGTCTCCTC CACGCCACCC ATGCGGCCCC AGATCACTGA CAGGAACTAC 
TTCCATCTGC CCTACCTGGA GCACAAACCC TGCATCTACA TCAAGAGCTG GTGGCCTGAC 
CAACGCCGTC GCCTGTACAA TGCCAACATC ATGGACCACA TCGCAGACAA GCTGGAAGAA 
GGCCTGAATG ATGTGCAGGA GATGATCAAA ACGGAGAAGT CGTACCCTGA GCGTCGACTT 
CGGGGCGTCC TAGAGGAGCT GAGCTCTGGT TGCTACCGCT TCCTCTCCCT CGCGGACAAG 
GACCAGGGCC ACTCATCCCG CACCAGGCTG GACCGGGAGC GTCTCAAGTC CTGCATGCGG 
GAGCTGGAAA GCATGGGGCA GCAGGCCAAG ACCCTGCGGG CACAAGTGAA GCGGCACACG 
GTGCGCGACA AGCTGAGGCT CTGTCAGAAC TTCCTGCAGA AGCTGCGCTT CCTGGCAGAC 
GAGCCCCAGC ACAGCATCCC TGACGTCTTC ATCTGGATGA TGAGCAACAA CAAGCGCATT 
GCCTACACCC GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTGGAGGA GGAACTGGGC 
AAGGACTGTG CCAAGGTCAA GACACTCTTC CTCAAGGTGC CGGAAGGGGA ATGGATTAGC 
AGCGGGTGGA GGGGCTGGGG GCTGGGCTAC TCTCAAGAGG TCATCTTCCT CTTGGAGGGA 
GCAGCATCCA CACCCCTGGG TCTGCCAGGC CTGAGTGGAC AAGATGCTCA GGGAAGAGCT 
AAAGAGAATA GGAAGGGGGA TCCCGCCCTG CCCATCCCCT TCTGTGCTGT GTTTCCCCAC 
TGCACAGCCC AGTGGGATGG CTGCGGTGGG GATGGGCCCC CTCTGACCAC AGCTTCTTCA 
GCTCCCTGCC CCTTCCTCAC CCCGAGGGCT CTGGCAGCTG ACCCTGAGCC CACCCCCCAG 
CTGCCAGGGA AGCGGGGCTT CGGCTCGGCA GGCTGGACAG TGCAGGCCAA GCTGGAGCTC 
TACCTGTGGC TGGGTCTAAG CAAGCAGCGC AAGGACTTCC TGTGTGGCCT GCCCTGCGGC 
TTCGAGGAGG TCAAGGCAGC CCAGGGCCTG GGCCTACTCT CCTTCCCGCC CATCAGCCTG 
GTCTACACTA AGAAGCAGGC CTTCCAGCTC CGCGCCCACA TGTACCAGGC TCGCAGCCTC 
TTTGCTGCTG ACAGCACCGG CCTCTCAGAC CCCTTTGCCC GTGTCTTCTT CATCAACCAG 
AGTCAGTGCA CAGAGGTACT GAATGAGACC CTGTGCCCCA CCTGGGACCA GATGCTGGTG 
TTCGACAACC TGGAGCTCTA CGGTGAAGCT CATGAGCTGC GGGACGATCC CCCCATCATT 
GTTATCGAAA TCTACGACCA GGACACCATG GGCAAAGCCG ACTTCATGGG CCGGACCTTT 
GCCAAGCCGC TGGTGAAGAT GGCCGACGAG GCGTACTGCC CACCCCGCTT CCCGCCCCAG 
CTGGAGTACT ATCAGATCTA CCGGGGCAAT GCCACGGCCG GGGACCTGCT GGCTGCCTTC 
GAGCTGCTGC AGATTGGACC AGCAGGGAAG GCCGACCTGC CGCCGATCAA CGGCCCAGTG 
GACATGGACC GAGGTCCCAT CATGCCTGTG CCTGTGGGCA TCCGACCCGT GCTTAGCAAG 
TACCGGGTGG AGGTGCTGTT CTGGGGCCTG CGAGACCTGA AGCGGGTAAA CCTGGCCCAG 
GTGGACAGGC CGCGGGTGGA CATCGAGTGC GCGGGGAAGG GGGTGCAGTC CTCCCTGATC 
CACAATTACA AGAAGAACCC CAACTTCAGC ACCCTGGTCA AGTGGTTTGA AGTGGACCTC 
CCAGAGAACG AGCTCCTGCA CCCGCCCTTG AACATCCGCG TGGTGGACTG CCGGGCCTTC 
GGCCGCTACA CCCTGGTCGG CTCCCACGCT GTCAGCTCCC TGCGGCGCTT CATCTACCGA 
CCCCCGGACC GCTCAGCTCC CAACTGGAAC ACTTCGGGCA GGCTCCTCCA GGGCCGCCGT 
GGGCTGTACA GTGGGGGCCC CTCCTCTCGC CTCACAGGGG AGGTCGTGGT GAGCATGGAG 
CCGGAGGTGC CCATCAAGAA GCTGGAGACC ATGGTGAAGC TGGACGCGAC TTCCGATGCC 
GTCATCAAGG TGGATGTGGC TGAGGAAGAG AAGGAGAGGA AGAAGAAGAA GAAGAAGGGC 
AGCTCAGATG AGCCGGAGGA GGAGGAGCCA GATGAGAGTA TGTTGGACTG GTGGTCCAAG 
TACTTTGCCT CCATTGACAC CATGAAAGAG CAAATTCGAC AACAAGAAAT CTCAGGAATT 
GACCTGGAGG AGAAGGAGGA GATGGACAAC ACTGAAGGCC TGAAGGGGCC GATGAAGGGC 
AAGGAGAAGG CAAGGGCTGC AAAGGAGGAG AAGAAGAAAA GAACCCAGAG CCTAGGCCCT 
GGCCAGGGGT CGGAGGCCCC CGAGAAGAGG AAACCCAAGA TTGATGAGCT CAAGGTGTAC 
CCCAAGGAGC TGGAGTCAGA GTTCGACAAC TTCGAGGACT GGCTGCACAC GTTCAACCTG 
CTTCGGGGGA AGACGGGGGA CGACGAGGAT GGCGGGACCG AGGAGGAGCG CATTGTGGGC 
CGGTTCAAGG GCTCCCTCTG CGTGTACAAA GTGCCACTCC CAGAGGACGT GTCCCGGGAA 
GCCGGCTACG ACCCCACCTA CGGCATGTTC CAGGGCATCC CCAGCAATGA CCCCATCAAC 
GTGCTGGTCC GGGTCTACGT GGTCCGGGCC ACGGACCTGC ACCCCGCAGA CATCAACGGC 
AAAGCTGACC CGTACATCGC CATCCGGCTA GGCAAGACTG ATATCCGTGA CAAGGAGAAC 
TACATCTCCA AGCAGCTCAA CCCCGTCTTT GGGAAGTCCT TTGACATCGA AGCCTGCTTC 
CCCATGGAAT CCATGCTGAC GGTGGCCGTG TACGACTGGG ATCTGGTAGG CACTGATGAC 
CTCATTGGGG AAACCAAGAT CGACCTGGAA AACCGCTTCT ACAGCAAGCA CCGCGCCACC 
TGCGGCATCG CCCAGACCTA CTCCGTACAC GGCTACAATA TCTGGCGGGA CCCTATGAAG 
CCCAGCCAGA TCCTGACCCG CCTCTGCAAG GAGGGCAAAG TGGACGGCCC CCACTTCGGG 
CCCCCCGGGA GAGTGAAGGT GTCCAACCGC ATCTTCACAG GGCCCTCTGA GATCGAGGAC 
GAGAATGGCC AGAGGAAGCC CACAGAAGAG CACGTGGCAC TGCTGGCCCT GAGACACTGG 
GAGGACATCC CCCGCGTGGG CTGTCGCCTG GTGCCCGAAC ATGTGGAGAC ACGGCCGCTG 
CTCAACCCCG ACAAGCCGGG CATCGAACAG GGCCGCCTGG AGCTGTGGGT GGACATGTTT 
CCCATGGACA TGCCGTCCCC CGGGACACCT CTGGACATCT CCCCTCGGAA GCCCAAGAAG 
TACGAGCTGA GGGTCATCGT GTGGAACACG GATGAGGTGG TCTTGGAAGA TGACGACTTC 
TTCACTGGGG AGAAGTCCAG CGACATTTTC GTGCGGGGGT GGCTGAAGGG CCAGCAGGAG 
GACAAGCAGG ACACAGACGT CCACTACCAC TCCCTCACGG GCGAGGGCAA CTTCAACTGG 
CGCTACCTGT TCCCCTTCGA CTACCTGGCT GCCGAGGAGA AGATAGTCAT CTCCAAGAAG 
GAGTCCATGT TCTCCTGGGA CGAGACCGAA TACAAGATCC CCGCACGGCT CACCCTGCAG 
ATCTGGGACG CCGACCACTT CTCTGCCGAT GACTTCCTGG GGGCCATCGA GCTGGACCTG 
AATCGCTTCC CTCGGGGCGC GAAAACGGCC AAGCAGTGCA CCATGGAGAT GGCCACAGGG 
GAGGTGGATG TGCCCCTGGT TTCCATCTTC AAGCAGAAGC GTGTCAAAGG CTGGTGGCCC 
CTCCTAGCCC GCAACGAGAA TGACGAGTTT GAGCTCACGG GCAAAGTGGA AGCAGAGTTG 
CATCTACTCA CCGCCGAGGA GGCGGAAAAG AATCCCGTGG GCCTGGCCCG CAATGAACCT 
GATCCCCTAG AGAAACCCAA CCGGCCCGAC ACCGCCTTCG TCTGGTTCCT CAACCCCCTC 
AAGTCCATCA AGTACCTCAT CTGCACGCGG TACAAGTGGC TCATCATCAA GATCGTGTTG 
GCGCTGCTGG GACTGCTCAT GCTGGCGCTC TTCCTCTACA GCCTCCCCGG TTACATGGTC 
AAGAAGCTCT TGGGGGCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329031.1
CDS	123..6443
Translation	MALLVHLKTVSELRGKGDRIAKVTFRGQSFYSRVLESCEDVADFDETFRWPVASSIDSNEMLEIQIFNYSKVFSNKLIGTFRMVLQKVVEENHVEVTDTLMDDNNAIIKTSLCVEVRYQATDGTVGSWDDGDFLGEESLHEEEKDSQETDGLLPGSRPSSRPSGEKSFRRAGRSVFSAMKLGKNRPHKEEPQRQDEPAVLEMEDLDRLAIRLGDGLDPDSVSLASVTALTTNVSNKRSKPDIKMEPSAGRPMDYQVSVTVIEARQLVGLNMDPVVCVEVGDEKKYTSMKESTNCPYYNEYFVFDFHVSPDVMFDKIIKISVIHSKNLLRSGTLVGSFKMDVGTVYSQPEHQFHHKWAVLSDPDDISAGLKGYVKCDVAVVGKGDNIKTPHKANETDEDDIEGNLLLPEGVPPERQWARFYVKIYRAEGLPRMNTSLMANVKKAFTGENKDLVDPYVQVFFAGQKGKTSVQKSSYEPLWNEQVVFTDLFPPLCKRMKVQIRDSDKVNDVAIGTHFIDLRKISNDGDKGFLPTLGPAWVNMYGSTRNYTLLDEHQDLNEGLGEGVSFRARLMLGLAVEILDTSNPEITSSTEVQVEQATPISESCTGKIEEFFLFGAFLEASMIDRKNGDKPITFEVTVGNYGNEVDGLSRPHRPRPRKEPGDEEEVDLIQNLSDEETDEGGGLASVSSTPPMRPQITDRNYFHLPYLEHKPCIYIKSWWPDQRRRLYNANIMDHIADKLEEGLNDVQEMIKTEKSYPERRLRGVLEELSSGCYRFLSLADKDQGHSSRTRLDRERLKSCMRELESMGQQAKTLRAQVKRHTVRDKLRLCQNFLQKLRFLADEPQHSIPDVFIWMMSNNKRIAYTRVPSKDLLFSIVEEELGKDCAKVKTLFLKVPEGEWISSGWRGWGLGYSQEVIFLLEGAASTPLGLPGLSGQDAQGRAKENRKGDPALPIPFCAVFPHCTAQWDGCGGDGPPLTTASSAPCPFLTPRALAADPEPTPQLPGKRGFGSAGWTVQAKLELYLWLGLSKQRKDFLCGLPCGFEEVKAAQGLGLLSFPPISLVYTKKQAFQLRAHMYQARSLFAADSTGLSDPFARVFFINQSQCTEVLNETLCPTWDQMLVFDNLELYGEAHELRDDPPIIVIEIYDQDTMGKADFMGRTFAKPLVKMADEAYCPPRFPPQLEYYQIYRGNATAGDLLAAFELLQIGPAGKADLPPINGPVDMDRGPIMPVPVGIRPVLSKYRVEVLFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSSLIHNYKKNPNFSTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHAVSSLRRFIYRPPDRSAPNWNTSGRLLQGRRGLYSGGPSSRLTGEVVVSMEPEVPIKKLETMVKLDATSDAVIKVDVAEEEKERKKKKKKGSSDEPEEEEPDESMLDWWSKYFASIDTMKEQIRQQEISGIDLEEKEEMDNTEGLKGPMKGKEKARAAKEEKKKRTQSLGPGQGSEAPEKRKPKIDELKVYPKELESEFDNFEDWLHTFNLLRGKTGDDEDGGTEEERIVGRFKGSLCVYKVPLPEDVSREAGYDPTYGMFQGIPSNDPINVLVRVYVVRATDLHPADINGKADPYIAIRLGKTDIRDKENYISKQLNPVFGKSFDIEACFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRFYSKHRATCGIAQTYSVHGYNIWRDPMKPSQILTRLCKEGKVDGPHFGPPGRVKVSNRIFTGPSEIEDENGQRKPTEEHVALLALRHWEDIPRVGCRLVPEHVETRPLLNPDKPGIEQGRLELWVDMFPMDMPSPGTPLDISPRKPKKYELRVIVWNTDEVVLEDDDFFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYLFPFDYLAAEEKIVISKKESMFSWDETEYKIPARLTLQIWDADHFSADDFLGAIELDLNRFPRGAKTAKQCTMEMATGEVDVPLVSIFKQKRVKGWWPLLARNENDEFELTGKVEAELHLLTAEEAEKNPVGLARNEPDPLEKPNRPDTAFVWFLNPLKSIKYLICTRYKWLIIKIVLALLGLLMLALFLYSLPGYMVKKLLGA

Target ORF information:

RefSeq Version	XM_015473545.1
Organism	Bos taurus(cattle)
Definition	Bos taurus otoferlin (OTOF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473545.1

ORF Insert Sequence:

ATGGCCCTGC TCGTCCACCT CAAGACAGTC TCCGAGCTGC GGGGCAAGGG TGACCGGATC 
GCCAAAGTGA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TCCTGGAGAG CTGTGAGGAT 
GTGGCTGACT TCGATGAGAC CTTCCGGTGG CCGGTGGCCA GCAGCATCGA CAGCAATGAG 
ATGCTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT GATCGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGAC CGACACGCTG 
ATGGATGACA ACAATGCAAT CATCAAGACC AGCCTGTGCG TGGAGGTCCG GTATCAGGCC 
ACAGATGGCA CAGTGGGCTC CTGGGATGAC GGGGACTTTC TGGGAGAGGA GTCCCTTCAC 
GAGGAAGAGA AGGACAGCCA GGAGACGGAT GGGCTGCTAC CCGGCTCCCG TCCCAGCTCC 
CGGCCATCAG GCGAGAAGAG TTTCCGGAGA GCCGGGAGAA GCGTGTTCTC CGCCATGAAG 
CTCGGCAAGA ACCGGCCTCA CAAGGAGGAA CCCCAAAGAC AAGATGAACC AGCTGTTCTG 
GAGATGGAGG ACCTTGACCG CCTGGCCATT CGGCTGGGGG ATGGGCTGGA CCCTGACTCC 
GTGTCGCTAG CATCCGTCAC AGCCCTCACC ACCAATGTCT CCAACAAGCG GTCTAAGCCA 
GACATCAAGA TGGAGCCAAG TGCCGGGCGG CCAATGGACT ACCAGGTCAG CGTCACAGTC 
ATCGAGGCTC GGCAGCTGGT GGGCTTGAAC ATGGACCCCG TGGTGTGTGT GGAGGTGGGC 
GATGAGAAGA AGTACACCTC CATGAAGGAG TCCACTAACT GCCCCTACTA TAATGAGTAC 
TTCGTCTTTG ACTTCCACGT CTCTCCTGAT GTCATGTTTG ACAAGATCAT CAAGATCTCG 
GTGATTCACT CCAAGAACCT GCTGCGCAGT GGCACCCTGG TGGGGTCCTT CAAAATGGAC 
GTGGGAACCG TGTACTCCCA GCCAGAGCAC CAGTTCCACC ACAAGTGGGC CGTCCTGTCC 
GACCCTGACG ACATCTCCGC GGGACTGAAG GGCTACGTGA AGTGCGATGT CGCTGTGGTG 
GGCAAGGGCG ACAACATTAA GACTCCCCAC AAGGCCAATG AGACGGATGA GGATGACATT 
GAGGGGAACT TGCTGCTCCC CGAAGGGGTG CCCCCTGAAC GCCAGTGGGC CCGGTTCTAC 
GTGAAGATCT ACCGAGCAGA GGGGCTGCCC CGCATGAACA CGAGCCTCAT GGCCAATGTG 
AAGAAGGCTT TCACTGGCGA GAACAAGGAT CTGGTCGACC CCTATGTGCA AGTCTTCTTT 
GCCGGCCAGA AGGGCAAGAC TTCAGTGCAG AAAAGCAGCT ACGAGCCACT GTGGAATGAG 
CAGGTGGTCT TCACAGACCT GTTTCCCCCA CTCTGCAAGC GCATGAAGGT GCAAATCCGG 
GACTCGGACA AGGTCAATGA CGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT 
TCCAACGATG GAGACAAAGG CTTCCTGCCC ACGCTGGGCC CTGCCTGGGT GAACATGTAC 
GGCTCCACAC GCAATTACAC GCTGCTAGAC GAGCATCAGG ACCTGAACGA GGGCCTGGGG 
GAGGGTGTGT CCTTCCGTGC CCGCCTCATG CTGGGCCTGG CGGTGGAGAT CCTGGACACC 
TCCAACCCTG AGATCACCAG CTCCACTGAG GTGCAGGTGG AGCAGGCCAC GCCCATCTCC 
GAGAGCTGCA CGGGGAAAAT AGAAGAATTC TTTCTATTTG GAGCCTTCCT GGAGGCCTCA 
ATGATCGACC GGAAAAATGG AGACAAGCCC ATCACCTTTG AGGTCACCGT AGGCAACTAC 
GGGAACGAAG TCGATGGGCT GTCACGACCC CATCGGCCTC GGCCTCGGAA GGAGCCTGGG 
GATGAGGAGG AAGTCGATCT GATCCAGAAC TTGAGTGATG AAGAGACTGA CGAGGGTGGG 
GGCCTGGCCT CGGTCTCCTC CACGCCACCC ATGCGGCCCC AGATCACTGA CAGGAACTAC 
TTCCATCTGC CCTACCTGGA GCACAAACCC TGCATCTACA TCAAGAGCTG GTGGCCTGAC 
CAACGCCGTC GCCTGTACAA TGCCAACATC ATGGACCACA TCGCAGACAA GCTGGAAGAA 
GGCCTGAATG ATGTGCAGGA GATGATCAAA ACGGAGAAGT CGTACCCTGA GCGTCGACTT 
CGGGGCGTCC TAGAGGAGCT GAGCTCTGGT TGCTACCGCT TCCTCTCCCT CGCGGACAAG 
GACCAGGGCC ACTCATCCCG CACCAGGCTG GACCGGGAGC GTCTCAAGTC CTGCATGCGG 
GAGCTGGAAA GCATGGGGCA GCAGGCCAAG ACCCTGCGGG CACAAGTGAA GCGGCACACG 
GTGCGCGACA AGCTGAGGCT CTGTCAGAAC TTCCTGCAGA AGCTGCGCTT CCTGGCAGAC 
GAGCCCCAGC ACAGCATCCC TGACGTCTTC ATCTGGATGA TGAGCAACAA CAAGCGCATT 
GCCTACACCC GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTGGAGGA GGAACTGGGC 
AAGGACTGTG CCAAGGTCAA GACACTCTTC CTCAAGGTGC CGGAAGGGGA ATGGATTAGC 
AGCGGGTGGA GGGGCTGGGG GCTGGGCTAC TCTCAAGAGG TCATCTTCCT CTTGGAGGGA 
GCAGCATCCA CACCCCTGGG TCTGCCAGGC CTGAGTGGAC AAGATGCTCA GGGAAGAGCT 
AAAGAGAATA GGAAGGGGGA TCCCGCCCTG CCCATCCCCT TCTGTGCTGT GTTTCCCCAC 
TGCACAGCCC AGTGGGATGG CTGCGGTGGG GATGGGCCCC CTCTGACCAC AGCTTCTTCA 
GCTCCCTGCC CCTTCCTCAC CCCGAGGGCT CTGGCAGCTG ACCCTGAGCC CACCCCCCAG 
CTGCCAGGGA AGCGGGGCTT CGGCTCGGCA GGCTGGACAG TGCAGGCCAA GCTGGAGCTC 
TACCTGTGGC TGGGTCTAAG CAAGCAGCGC AAGGACTTCC TGTGTGGCCT GCCCTGCGGC 
TTCGAGGAGG TCAAGGCAGC CCAGGGCCTG GGCCTACTCT CCTTCCCGCC CATCAGCCTG 
GTCTACACTA AGAAGCAGGC CTTCCAGCTC CGCGCCCACA TGTACCAGGC TCGCAGCCTC 
TTTGCTGCTG ACAGCACCGG CCTCTCAGAC CCCTTTGCCC GTGTCTTCTT CATCAACCAG 
AGTCAGTGCA CAGAGGTACT GAATGAGACC CTGTGCCCCA CCTGGGACCA GATGCTGGTG 
TTCGACAACC TGGAGCTCTA CGGTGAAGCT CATGAGCTGC GGGACGATCC CCCCATCATT 
GTTATCGAAA TCTACGACCA GGACACCATG GGCAAAGCCG ACTTCATGGG CCGGACCTTT 
GCCAAGCCGC TGGTGAAGAT GGCCGACGAG GCGTACTGCC CACCCCGCTT CCCGCCCCAG 
CTGGAGTACT ATCAGATCTA CCGGGGCAAT GCCACGGCCG GGGACCTGCT GGCTGCCTTC 
GAGCTGCTGC AGATTGGACC AGCAGGGAAG GCCGACCTGC CGCCGATCAA CGGCCCAGTG 
GACATGGACC GAGGTCCCAT CATGCCTGTG CCTGTGGGCA TCCGACCCGT GCTTAGCAAG 
TACCGGGTGG AGGTGCTGTT CTGGGGCCTG CGAGACCTGA AGCGGGTAAA CCTGGCCCAG 
GTGGACAGGC CGCGGGTGGA CATCGAGTGC GCGGGGAAGG GGGTGCAGTC CTCCCTGATC 
CACAATTACA AGAAGAACCC CAACTTCAGC ACCCTGGTCA AGTGGTTTGA AGTGGACCTC 
CCAGAGAACG AGCTCCTGCA CCCGCCCTTG AACATCCGCG TGGTGGACTG CCGGGCCTTC 
GGCCGCTACA CCCTGGTCGG CTCCCACGCT GTCAGCTCCC TGCGGCGCTT CATCTACCGA 
CCCCCGGACC GCTCAGCTCC CAACTGGAAC ACTTCGGGCA GGCTCCTCCA GGGCCGCCGT 
GGGCTGTACA GTGGGGGCCC CTCCTCTCGC CTCACAGGGG AGGTCGTGGT GAGCATGGAG 
CCGGAGGTGC CCATCAAGAA GCTGGAGACC ATGGTGAAGC TGGACGCGAC TTCCGATGCC 
GTCATCAAGG TGGATGTGGC TGAGGAAGAG AAGGAGAGGA AGAAGAAGAA GAAGAAGGGC 
AGCTCAGATG AGCCGGAGGA GGAGGAGCCA GATGAGAGTA TGTTGGACTG GTGGTCCAAG 
TACTTTGCCT CCATTGACAC CATGAAAGAG CAAATTCGAC AACAAGAAAT CTCAGGAATT 
GACCTGGAGG AGAAGGAGGA GATGGACAAC ACTGAAGGCC TGAAGGGGCC GATGAAGGGC 
AAGGAGAAGG CAAGGGCTGC AAAGGAGGAG AAGAAGAAAA GAACCCAGAG CCTAGGCCCT 
GGCCAGGGGT CGGAGGCCCC CGAGAAGAGG AAACCCAAGA TTGATGAGCT CAAGGTGTAC 
CCCAAGGAGC TGGAGTCAGA GTTCGACAAC TTCGAGGACT GGCTGCACAC GTTCAACCTG 
CTTCGGGGGA AGACGGGGGA CGACGAGGAT GGCGGGACCG AGGAGGAGCG CATTGTGGGC 
CGGTTCAAGG GCTCCCTCTG CGTGTACAAA GTGCCACTCC CAGAGGACGT GTCCCGGGAA 
GCCGGCTACG ACCCCACCTA CGGCATGTTC CAGGGCATCC CCAGCAATGA CCCCATCAAC 
GTGCTGGTCC GGGTCTACGT GGTCCGGGCC ACGGACCTGC ACCCCGCAGA CATCAACGGC 
AAAGCTGACC CGTACATCGC CATCCGGCTA GGCAAGACTG ATATCCGTGA CAAGGAGAAC 
TACATCTCCA AGCAGCTCAA CCCCGTCTTT GGGAAGTCCT TTGACATCGA AGCCTGCTTC 
CCCATGGAAT CCATGCTGAC GGTGGCCGTG TACGACTGGG ATCTGGTAGG CACTGATGAC 
CTCATTGGGG AAACCAAGAT CGACCTGGAA AACCGCTTCT ACAGCAAGCA CCGCGCCACC 
TGCGGCATCG CCCAGACCTA CTCCGTACAC GGCTACAATA TCTGGCGGGA CCCTATGAAG 
CCCAGCCAGA TCCTGACCCG CCTCTGCAAG GAGGGCAAAG TGGACGGCCC CCACTTCGGG 
CCCCCCGGGA GAGTGAAGGT GTCCAACCGC ATCTTCACAG GGCCCTCTGA GATCGAGGAC 
GAGAATGGCC AGAGGAAGCC CACAGAAGAG CACGTGGCAC TGCTGGCCCT GAGACACTGG 
GAGGACATCC CCCGCGTGGG CTGTCGCCTG GTGCCCGAAC ATGTGGAGAC ACGGCCGCTG 
CTCAACCCCG ACAAGCCGGG CATCGAACAG GGCCGCCTGG AGCTGTGGGT GGACATGTTT 
CCCATGGACA TGCCGTCCCC CGGGACACCT CTGGACATCT CCCCTCGGAA GCCCAAGAAG 
TACGAGCTGA GGGTCATCGT GTGGAACACG GATGAGGTGG TCTTGGAAGA TGACGACTTC 
TTCACTGGGG AGAAGTCCAG CGACATTTTC GTGCGGGGGT GGCTGAAGGG CCAGCAGGAG 
GACAAGCAGG ACACAGACGT CCACTACCAC TCCCTCACGG GCGAGGGCAA CTTCAACTGG 
CGCTACCTGT TCCCCTTCGA CTACCTGGCT GCCGAGGAGA AGATAGTCAT CTCCAAGAAG 
GAGTCCATGT TCTCCTGGGA CGAGACCGAA TACAAGATCC CCGCACGGCT CACCCTGCAG 
ATCTGGGACG CCGACCACTT CTCTGCCGAT GACTTCCTGG GGGCCATCGA GCTGGACCTG 
AATCGCTTCC CTCGGGGCGC GAAAACGGCC AAGCAGTGCA CCATGGAGAT GGCCACAGGG 
GAGGTGGATG TGCCCCTGGT TTCCATCTTC AAGCAGAAGC GTGTCAAAGG CTGGTGGCCC 
CTCCTAGCCC GCAACGAGAA TGACGAGTTT GAGCTCACGG GCAAAGTGGA AGCAGAGTTG 
CATCTACTCA CCGCCGAGGA GGCGGAAAAG AATCCCGTGG GCCTGGCCCG CAATGAACCT 
GATCCCCTAG AGAAACCCAA CCGGCCCGAC ACCGCCTTCG TCTGGTTCCT CAACCCCCTC 
AAGTCCATCA AGTACCTCAT CTGCACGCGG TACAAGTGGC TCATCATCAA GATCGTGTTG 
GCGCTGCTGG GACTGCTCAT GCTGGCGCTC TTCCTCTACA GCCTCCCCGG TTACATGGTC 
AAGAAGCTCT TGGGGGCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOF ( XM_015473546.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329032.1 Bos taurus otoferlin (OTOF), transcript variant X2, mRNA.

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CloneID	OBa91932
Clone ID Related Accession (Same CDS sequence)	XM_015473546.1 , XM_015473546.2
Accession Version	XM_015473546.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	otoferlin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTGC TCGTCCACCT CAAGACAGTC TCCGAGCTGC GGGGCAAGGG TGACCGGATC 
GCCAAAGTGA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TCCTGGAGAG CTGTGAGGAT 
GTGGCTGACT TCGATGAGAC CTTCCGGTGG CCGGTGGCCA GCAGCATCGA CAGCAATGAG 
ATGCTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT GATCGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGAC CGACACGCTG 
ATGGATGACA ACAATGCAAT CATCAAGACC AGCCTGTGCG TGGAGGTCCG GTATCAGGCC 
ACAGATGGCA CAGTGGGCTC CTGGGATGAC GGGGACTTTC TGGGAGAGGA GTCCCTTCAC 
GAGGAAGAGA AGGACAGCCA GGAGACGGAT GGGCTGCTAC CCGGCTCCCG TCCCAGCTCC 
CGGCCATCAG GCGAGAAGAG TTTCCGGAGA GCCGGGAGAA GCGTGTTCTC CGCCATGAAG 
CTCGGCAAGA ACCGGCCTCA CAAGGAGGAA CCCCAAAGAC AAGATGAACC AGCTGTTCTG 
GAGATGGAGG ACCTTGACCG CCTGGCCATT CGGCTGGGGG ATGGGCTGGA CCCTGACTCC 
GTGTCGCTAG CATCCGTCAC AGCCCTCACC ACCAATGTCT CCAACAAGCG GTCTAAGCCA 
GACATCAAGA TGGAGCCAAG TGCCGGGCGG CCAATGGACT ACCAGGTCAG CGTCACAGTC 
ATCGAGGCTC GGCAGCTGGT GGGCTTGAAC ATGGACCCCG TGGTGTGTGT GGAGGTGGGC 
GATGAGAAGA AGTACACCTC CATGAAGGAG TCCACTAACT GCCCCTACTA TAATGAGTAC 
TTCGTCTTTG ACTTCCACGT CTCTCCTGAT GTCATGTTTG ACAAGATCAT CAAGATCTCG 
GTGATTCACT CCAAGAACCT GCTGCGCAGT GGCACCCTGG TGGGGTCCTT CAAAATGGAC 
GTGGGAACCG TGTACTCCCA GCCAGAGCAC CAGTTCCACC ACAAGTGGGC CGTCCTGTCC 
GACCCTGACG ACATCTCCGC GGGACTGAAG GGCTACGTGA AGTGCGATGT CGCTGTGGTG 
GGCAAGGGCG ACAACATTAA GACTCCCCAC AAGGCCAATG AGACGGATGA GGATGACATT 
GAGGGGAACT TGCTGCTCCC CGAAGGGGTG CCCCCTGAAC GCCAGTGGGC CCGGTTCTAC 
GTGAAGATCT ACCGAGCAGA GGGGCTGCCC CGCATGAACA CGAGCCTCAT GGCCAATGTG 
AAGAAGGCTT TCACTGGCGA GAACAAGGAT CTGGTCGACC CCTATGTGCA AGTCTTCTTT 
GCCGGCCAGA AGGGCAAGAC TTCAGTGCAG AAAAGCAGCT ACGAGCCACT GTGGAATGAG 
CAGGTGGTCT TCACAGACCT GTTTCCCCCA CTCTGCAAGC GCATGAAGGT GCAAATCCGG 
GACTCGGACA AGGTCAATGA CGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT 
TCCAACGATG GAGACAAAGG CTTCCTGCCC ACGCTGGGCC CTGCCTGGGT GAACATGTAC 
GGCTCCACAC GCAATTACAC GCTGCTAGAC GAGCATCAGG ACCTGAACGA GGGCCTGGGG 
GAGGGTGTGT CCTTCCGTGC CCGCCTCATG CTGGGCCTGG CGGTGGAGAT CCTGGACACC 
TCCAACCCTG AGATCACCAG CTCCACTGAG GTGCAGGTGG AGCAGGCCAC GCCCATCTCC 
GAGAGCTGCA CGGGGAAAAT AGAAGAATTC TTTCTATTTG GAGCCTTCCT GGAGGCCTCA 
ATGATCGACC GGAAAAATGG AGACAAGCCC ATCACCTTTG AGGTCACCGT AGGCAACTAC 
GGGAACGAAG TCGATGGGCT GTCACGACCC CATCGGCCTC GGCCTCGGAA GGAGCCTGGG 
GATGAGGAGG AAGTCGATCT GATCCAGAAC TTGAGTGATG AAGAGACTGA CGAGGGTGGG 
GGCCTGGCCT CGGTCTCCTC CACGCCACCC ATGCGGCCCC AGATCACTGA CAGGAACTAC 
TTCCATCTGC CCTACCTGGA GCACAAACCC TGCATCTACA TCAAGAGCTG GTGGCCTGAC 
CAACGCCGTC GCCTGTACAA TGCCAACATC ATGGACCACA TCGCAGACAA GCTGGAAGAA 
GGCCTGAATG ATGTGCAGGA GATGATCAAA ACGGAGAAGT CGTACCCTGA GCGTCGACTT 
CGGGGCGTCC TAGAGGAGCT GAGCTCTGGT TGCTACCGCT TCCTCTCCCT CGCGGACAAG 
GACCAGGGCC ACTCATCCCG CACCAGGCTG GACCGGGAGC GTCTCAAGTC CTGCATGCGG 
GAGCTGGAAA GCATGGGGCA GCAGGCCAAG ACCCTGCGGG CACAAGTGAA GCGGCACACG 
GTGCGCGACA AGCTGAGGCT CTGTCAGAAC TTCCTGCAGA AGCTGCGCTT CCTGGCAGAC 
GAGCCCCAGC ACAGCATCCC TGACGTCTTC ATCTGGATGA TGAGCAACAA CAAGCGCATT 
GCCTACACCC GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTGGAGGA GGAACTGGGC 
AAGGACTGTG CCAAGGTCAA GACACTCTTC CTCAAGGTGC CGGAAGGGGA ATGGATTAGC 
AGCGGGTGGA GGGGCTGGGG GCTGGGCTAC TCTCAAGAGG TCATCTTCCT CTTGGAGGGA 
GCAGCATCCA CACCCCTGGG TCTGCCAGGC CTGAGTGGAC AAGATGCTCA GGGAAGAGCT 
AAAGAGAATA GGAAGGGGGA TCCCGCCCTG CCCATCCCCT TCTGTGCTGT GTTTCCCCAC 
TGCACAGCCC AGTGGGATGG CTGCGGTGGG GATGGGCCCC CTCTGACCAC AGCTTCTTCA 
GCTCCCTGCC CCTTCCTCAC CCCGAGGGCT CTGGCAGCTG ACCCTGAGCC CACCCCCCAG 
CTGCCAGGGA AGCGGGGCTT CGGCTCGGCA GGCTGGACAG TGCAGGCCAA GCTGGAGCTC 
TACCTGTGGC TGGGTCTAAG CAAGCAGCGC AAGGACTTCC TGTGTGGCCT GCCCTGCGGC 
TTCGAGGAGG TCAAGGCAGC CCAGGGCCTG GGCCTACTCT CCTTCCCGCC CATCAGCCTG 
GTCTACACTA AGAAGCAGGC CTTCCAGCTC CGCGCCCACA TGTACCAGGC TCGCAGCCTC 
TTTGCTGCTG ACAGCACCGG CCTCTCAGAC CCCTTTGCCC GTGTCTTCTT CATCAACCAG 
AGTCAGTGCA CAGAGGTACT GAATGAGACC CTGTGCCCCA CCTGGGACCA GATGCTGGTG 
TTCGACAACC TGGAGCTCTA CGGTGAAGCT CATGAGCTGC GGGACGATCC CCCCATCATT 
GTTATCGAAA TCTACGACCA GGACACCATG GGCAAAGCCG ACTTCATGGG CCGGACCTTT 
GCCAAGCCGC TGGTGAAGAT GGCCGACGAG GCGTACTGCC CACCCCGCTT CCCGCCCCAG 
CTGGAGTACT ATCAGATCTA CCGGGGCAAT GCCACGGCCG GGGACCTGCT GGCTGCCTTC 
GAGCTGCTGC AGATTGGACC AGCAGGGAAG GCCGACCTGC CGCCGATCAA CGGCCCAGTG 
GACATGGACC GAGGTCCCAT CATGCCTGTG CCTGTGGGCA TCCGACCCGT GCTTAGCAAG 
TACCGGGTGG AGGTGCTGTT CTGGGGCCTG CGAGACCTGA AGCGGGTAAA CCTGGCCCAG 
GTGGACAGGC CGCGGGTGGA CATCGAGTGC GCGGGGAAGG GGGTGCAGTC CTCCCTGATC 
CACAATTACA AGAAGAACCC CAACTTCAGC ACCCTGGTCA AGTGGTTTGA AGTGGACCTC 
CCAGAGAACG AGCTCCTGCA CCCGCCCTTG AACATCCGCG TGGTGGACTG CCGGGCCTTC 
GGCCGCTACA CCCTGGTCGG CTCCCACGCT GTCAGCTCCC TGCGGCGCTT CATCTACCGA 
CCCCCGGACC GCTCAGCTCC CAACTGGAAC ACTTCGGGGG AGGTCGTGGT GAGCATGGAG 
CCGGAGGTGC CCATCAAGAA GCTGGAGACC ATGGTGAAGC TGGACGCGAC TTCCGATGCC 
GTCATCAAGG TGGATGTGGC TGAGGAAGAG AAGGAGAGGA AGAAGAAGAA GAAGAAGGGC 
AGCTCAGATG AGCCGGAGGA GGAGGAGCCA GATGAGAGTA TGTTGGACTG GTGGTCCAAG 
TACTTTGCCT CCATTGACAC CATGAAAGAG CAAATTCGAC AACAAGAAAT CTCAGGAATT 
GACCTGGAGG AGAAGGAGGA GATGGACAAC ACTGAAGGCC TGAAGGGGCC GATGAAGGGC 
AAGGAGAAGG CAAGGGCTGC AAAGGAGGAG AAGAAGAAAA GAACCCAGAG CCTAGGCCCT 
GGCCAGGGGT CGGAGGCCCC CGAGAAGAGG AAACCCAAGA TTGATGAGCT CAAGGTGTAC 
CCCAAGGAGC TGGAGTCAGA GTTCGACAAC TTCGAGGACT GGCTGCACAC GTTCAACCTG 
CTTCGGGGGA AGACGGGGGA CGACGAGGAT GGCGGGACCG AGGAGGAGCG CATTGTGGGC 
CGGTTCAAGG GCTCCCTCTG CGTGTACAAA GTGCCACTCC CAGAGGACGT GTCCCGGGAA 
GCCGGCTACG ACCCCACCTA CGGCATGTTC CAGGGCATCC CCAGCAATGA CCCCATCAAC 
GTGCTGGTCC GGGTCTACGT GGTCCGGGCC ACGGACCTGC ACCCCGCAGA CATCAACGGC 
AAAGCTGACC CGTACATCGC CATCCGGCTA GGCAAGACTG ATATCCGTGA CAAGGAGAAC 
TACATCTCCA AGCAGCTCAA CCCCGTCTTT GGGAAGTCCT TTGACATCGA AGCCTGCTTC 
CCCATGGAAT CCATGCTGAC GGTGGCCGTG TACGACTGGG ATCTGGTAGG CACTGATGAC 
CTCATTGGGG AAACCAAGAT CGACCTGGAA AACCGCTTCT ACAGCAAGCA CCGCGCCACC 
TGCGGCATCG CCCAGACCTA CTCCGTACAC GGCTACAATA TCTGGCGGGA CCCTATGAAG 
CCCAGCCAGA TCCTGACCCG CCTCTGCAAG GAGGGCAAAG TGGACGGCCC CCACTTCGGG 
CCCCCCGGGA GAGTGAAGGT GTCCAACCGC ATCTTCACAG GGCCCTCTGA GATCGAGGAC 
GAGAATGGCC AGAGGAAGCC CACAGAAGAG CACGTGGCAC TGCTGGCCCT GAGACACTGG 
GAGGACATCC CCCGCGTGGG CTGTCGCCTG GTGCCCGAAC ATGTGGAGAC ACGGCCGCTG 
CTCAACCCCG ACAAGCCGGG CATCGAACAG GGCCGCCTGG AGCTGTGGGT GGACATGTTT 
CCCATGGACA TGCCGTCCCC CGGGACACCT CTGGACATCT CCCCTCGGAA GCCCAAGAAG 
TACGAGCTGA GGGTCATCGT GTGGAACACG GATGAGGTGG TCTTGGAAGA TGACGACTTC 
TTCACTGGGG AGAAGTCCAG CGACATTTTC GTGCGGGGGT GGCTGAAGGG CCAGCAGGAG 
GACAAGCAGG ACACAGACGT CCACTACCAC TCCCTCACGG GCGAGGGCAA CTTCAACTGG 
CGCTACCTGT TCCCCTTCGA CTACCTGGCT GCCGAGGAGA AGATAGTCAT CTCCAAGAAG 
GAGTCCATGT TCTCCTGGGA CGAGACCGAA TACAAGATCC CCGCACGGCT CACCCTGCAG 
ATCTGGGACG CCGACCACTT CTCTGCCGAT GACTTCCTGG GGGCCATCGA GCTGGACCTG 
AATCGCTTCC CTCGGGGCGC GAAAACGGCC AAGCAGTGCA CCATGGAGAT GGCCACAGGG 
GAGGTGGATG TGCCCCTGGT TTCCATCTTC AAGCAGAAGC GTGTCAAAGG CTGGTGGCCC 
CTCCTAGCCC GCAACGAGAA TGACGAGTTT GAGCTCACGG GCAAAGTGGA AGCAGAGTTG 
CATCTACTCA CCGCCGAGGA GGCGGAAAAG AATCCCGTGG GCCTGGCCCG CAATGAACCT 
GATCCCCTAG AGAAACCCAA CCGGCCCGAC ACCGCCTTCG TCTGGTTCCT CAACCCCCTC 
AAGTCCATCA AGTACCTCAT CTGCACGCGG TACAAGTGGC TCATCATCAA GATCGTGTTG 
GCGCTGCTGG GACTGCTCAT GCTGGCGCTC TTCCTCTACA GCCTCCCCGG TTACATGGTC 
AAGAAGCTCT TGGGGGCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329032.1
CDS	123..6383
Translation	MALLVHLKTVSELRGKGDRIAKVTFRGQSFYSRVLESCEDVADFDETFRWPVASSIDSNEMLEIQIFNYSKVFSNKLIGTFRMVLQKVVEENHVEVTDTLMDDNNAIIKTSLCVEVRYQATDGTVGSWDDGDFLGEESLHEEEKDSQETDGLLPGSRPSSRPSGEKSFRRAGRSVFSAMKLGKNRPHKEEPQRQDEPAVLEMEDLDRLAIRLGDGLDPDSVSLASVTALTTNVSNKRSKPDIKMEPSAGRPMDYQVSVTVIEARQLVGLNMDPVVCVEVGDEKKYTSMKESTNCPYYNEYFVFDFHVSPDVMFDKIIKISVIHSKNLLRSGTLVGSFKMDVGTVYSQPEHQFHHKWAVLSDPDDISAGLKGYVKCDVAVVGKGDNIKTPHKANETDEDDIEGNLLLPEGVPPERQWARFYVKIYRAEGLPRMNTSLMANVKKAFTGENKDLVDPYVQVFFAGQKGKTSVQKSSYEPLWNEQVVFTDLFPPLCKRMKVQIRDSDKVNDVAIGTHFIDLRKISNDGDKGFLPTLGPAWVNMYGSTRNYTLLDEHQDLNEGLGEGVSFRARLMLGLAVEILDTSNPEITSSTEVQVEQATPISESCTGKIEEFFLFGAFLEASMIDRKNGDKPITFEVTVGNYGNEVDGLSRPHRPRPRKEPGDEEEVDLIQNLSDEETDEGGGLASVSSTPPMRPQITDRNYFHLPYLEHKPCIYIKSWWPDQRRRLYNANIMDHIADKLEEGLNDVQEMIKTEKSYPERRLRGVLEELSSGCYRFLSLADKDQGHSSRTRLDRERLKSCMRELESMGQQAKTLRAQVKRHTVRDKLRLCQNFLQKLRFLADEPQHSIPDVFIWMMSNNKRIAYTRVPSKDLLFSIVEEELGKDCAKVKTLFLKVPEGEWISSGWRGWGLGYSQEVIFLLEGAASTPLGLPGLSGQDAQGRAKENRKGDPALPIPFCAVFPHCTAQWDGCGGDGPPLTTASSAPCPFLTPRALAADPEPTPQLPGKRGFGSAGWTVQAKLELYLWLGLSKQRKDFLCGLPCGFEEVKAAQGLGLLSFPPISLVYTKKQAFQLRAHMYQARSLFAADSTGLSDPFARVFFINQSQCTEVLNETLCPTWDQMLVFDNLELYGEAHELRDDPPIIVIEIYDQDTMGKADFMGRTFAKPLVKMADEAYCPPRFPPQLEYYQIYRGNATAGDLLAAFELLQIGPAGKADLPPINGPVDMDRGPIMPVPVGIRPVLSKYRVEVLFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSSLIHNYKKNPNFSTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHAVSSLRRFIYRPPDRSAPNWNTSGEVVVSMEPEVPIKKLETMVKLDATSDAVIKVDVAEEEKERKKKKKKGSSDEPEEEEPDESMLDWWSKYFASIDTMKEQIRQQEISGIDLEEKEEMDNTEGLKGPMKGKEKARAAKEEKKKRTQSLGPGQGSEAPEKRKPKIDELKVYPKELESEFDNFEDWLHTFNLLRGKTGDDEDGGTEEERIVGRFKGSLCVYKVPLPEDVSREAGYDPTYGMFQGIPSNDPINVLVRVYVVRATDLHPADINGKADPYIAIRLGKTDIRDKENYISKQLNPVFGKSFDIEACFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRFYSKHRATCGIAQTYSVHGYNIWRDPMKPSQILTRLCKEGKVDGPHFGPPGRVKVSNRIFTGPSEIEDENGQRKPTEEHVALLALRHWEDIPRVGCRLVPEHVETRPLLNPDKPGIEQGRLELWVDMFPMDMPSPGTPLDISPRKPKKYELRVIVWNTDEVVLEDDDFFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYLFPFDYLAAEEKIVISKKESMFSWDETEYKIPARLTLQIWDADHFSADDFLGAIELDLNRFPRGAKTAKQCTMEMATGEVDVPLVSIFKQKRVKGWWPLLARNENDEFELTGKVEAELHLLTAEEAEKNPVGLARNEPDPLEKPNRPDTAFVWFLNPLKSIKYLICTRYKWLIIKIVLALLGLLMLALFLYSLPGYMVKKLLGA

Target ORF information:

RefSeq Version	XM_015473546.1
Organism	Bos taurus(cattle)
Definition	Bos taurus otoferlin (OTOF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473546.1

ORF Insert Sequence:

ATGGCCCTGC TCGTCCACCT CAAGACAGTC TCCGAGCTGC GGGGCAAGGG TGACCGGATC 
GCCAAAGTGA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TCCTGGAGAG CTGTGAGGAT 
GTGGCTGACT TCGATGAGAC CTTCCGGTGG CCGGTGGCCA GCAGCATCGA CAGCAATGAG 
ATGCTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT GATCGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGAC CGACACGCTG 
ATGGATGACA ACAATGCAAT CATCAAGACC AGCCTGTGCG TGGAGGTCCG GTATCAGGCC 
ACAGATGGCA CAGTGGGCTC CTGGGATGAC GGGGACTTTC TGGGAGAGGA GTCCCTTCAC 
GAGGAAGAGA AGGACAGCCA GGAGACGGAT GGGCTGCTAC CCGGCTCCCG TCCCAGCTCC 
CGGCCATCAG GCGAGAAGAG TTTCCGGAGA GCCGGGAGAA GCGTGTTCTC CGCCATGAAG 
CTCGGCAAGA ACCGGCCTCA CAAGGAGGAA CCCCAAAGAC AAGATGAACC AGCTGTTCTG 
GAGATGGAGG ACCTTGACCG CCTGGCCATT CGGCTGGGGG ATGGGCTGGA CCCTGACTCC 
GTGTCGCTAG CATCCGTCAC AGCCCTCACC ACCAATGTCT CCAACAAGCG GTCTAAGCCA 
GACATCAAGA TGGAGCCAAG TGCCGGGCGG CCAATGGACT ACCAGGTCAG CGTCACAGTC 
ATCGAGGCTC GGCAGCTGGT GGGCTTGAAC ATGGACCCCG TGGTGTGTGT GGAGGTGGGC 
GATGAGAAGA AGTACACCTC CATGAAGGAG TCCACTAACT GCCCCTACTA TAATGAGTAC 
TTCGTCTTTG ACTTCCACGT CTCTCCTGAT GTCATGTTTG ACAAGATCAT CAAGATCTCG 
GTGATTCACT CCAAGAACCT GCTGCGCAGT GGCACCCTGG TGGGGTCCTT CAAAATGGAC 
GTGGGAACCG TGTACTCCCA GCCAGAGCAC CAGTTCCACC ACAAGTGGGC CGTCCTGTCC 
GACCCTGACG ACATCTCCGC GGGACTGAAG GGCTACGTGA AGTGCGATGT CGCTGTGGTG 
GGCAAGGGCG ACAACATTAA GACTCCCCAC AAGGCCAATG AGACGGATGA GGATGACATT 
GAGGGGAACT TGCTGCTCCC CGAAGGGGTG CCCCCTGAAC GCCAGTGGGC CCGGTTCTAC 
GTGAAGATCT ACCGAGCAGA GGGGCTGCCC CGCATGAACA CGAGCCTCAT GGCCAATGTG 
AAGAAGGCTT TCACTGGCGA GAACAAGGAT CTGGTCGACC CCTATGTGCA AGTCTTCTTT 
GCCGGCCAGA AGGGCAAGAC TTCAGTGCAG AAAAGCAGCT ACGAGCCACT GTGGAATGAG 
CAGGTGGTCT TCACAGACCT GTTTCCCCCA CTCTGCAAGC GCATGAAGGT GCAAATCCGG 
GACTCGGACA AGGTCAATGA CGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT 
TCCAACGATG GAGACAAAGG CTTCCTGCCC ACGCTGGGCC CTGCCTGGGT GAACATGTAC 
GGCTCCACAC GCAATTACAC GCTGCTAGAC GAGCATCAGG ACCTGAACGA GGGCCTGGGG 
GAGGGTGTGT CCTTCCGTGC CCGCCTCATG CTGGGCCTGG CGGTGGAGAT CCTGGACACC 
TCCAACCCTG AGATCACCAG CTCCACTGAG GTGCAGGTGG AGCAGGCCAC GCCCATCTCC 
GAGAGCTGCA CGGGGAAAAT AGAAGAATTC TTTCTATTTG GAGCCTTCCT GGAGGCCTCA 
ATGATCGACC GGAAAAATGG AGACAAGCCC ATCACCTTTG AGGTCACCGT AGGCAACTAC 
GGGAACGAAG TCGATGGGCT GTCACGACCC CATCGGCCTC GGCCTCGGAA GGAGCCTGGG 
GATGAGGAGG AAGTCGATCT GATCCAGAAC TTGAGTGATG AAGAGACTGA CGAGGGTGGG 
GGCCTGGCCT CGGTCTCCTC CACGCCACCC ATGCGGCCCC AGATCACTGA CAGGAACTAC 
TTCCATCTGC CCTACCTGGA GCACAAACCC TGCATCTACA TCAAGAGCTG GTGGCCTGAC 
CAACGCCGTC GCCTGTACAA TGCCAACATC ATGGACCACA TCGCAGACAA GCTGGAAGAA 
GGCCTGAATG ATGTGCAGGA GATGATCAAA ACGGAGAAGT CGTACCCTGA GCGTCGACTT 
CGGGGCGTCC TAGAGGAGCT GAGCTCTGGT TGCTACCGCT TCCTCTCCCT CGCGGACAAG 
GACCAGGGCC ACTCATCCCG CACCAGGCTG GACCGGGAGC GTCTCAAGTC CTGCATGCGG 
GAGCTGGAAA GCATGGGGCA GCAGGCCAAG ACCCTGCGGG CACAAGTGAA GCGGCACACG 
GTGCGCGACA AGCTGAGGCT CTGTCAGAAC TTCCTGCAGA AGCTGCGCTT CCTGGCAGAC 
GAGCCCCAGC ACAGCATCCC TGACGTCTTC ATCTGGATGA TGAGCAACAA CAAGCGCATT 
GCCTACACCC GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTGGAGGA GGAACTGGGC 
AAGGACTGTG CCAAGGTCAA GACACTCTTC CTCAAGGTGC CGGAAGGGGA ATGGATTAGC 
AGCGGGTGGA GGGGCTGGGG GCTGGGCTAC TCTCAAGAGG TCATCTTCCT CTTGGAGGGA 
GCAGCATCCA CACCCCTGGG TCTGCCAGGC CTGAGTGGAC AAGATGCTCA GGGAAGAGCT 
AAAGAGAATA GGAAGGGGGA TCCCGCCCTG CCCATCCCCT TCTGTGCTGT GTTTCCCCAC 
TGCACAGCCC AGTGGGATGG CTGCGGTGGG GATGGGCCCC CTCTGACCAC AGCTTCTTCA 
GCTCCCTGCC CCTTCCTCAC CCCGAGGGCT CTGGCAGCTG ACCCTGAGCC CACCCCCCAG 
CTGCCAGGGA AGCGGGGCTT CGGCTCGGCA GGCTGGACAG TGCAGGCCAA GCTGGAGCTC 
TACCTGTGGC TGGGTCTAAG CAAGCAGCGC AAGGACTTCC TGTGTGGCCT GCCCTGCGGC 
TTCGAGGAGG TCAAGGCAGC CCAGGGCCTG GGCCTACTCT CCTTCCCGCC CATCAGCCTG 
GTCTACACTA AGAAGCAGGC CTTCCAGCTC CGCGCCCACA TGTACCAGGC TCGCAGCCTC 
TTTGCTGCTG ACAGCACCGG CCTCTCAGAC CCCTTTGCCC GTGTCTTCTT CATCAACCAG 
AGTCAGTGCA CAGAGGTACT GAATGAGACC CTGTGCCCCA CCTGGGACCA GATGCTGGTG 
TTCGACAACC TGGAGCTCTA CGGTGAAGCT CATGAGCTGC GGGACGATCC CCCCATCATT 
GTTATCGAAA TCTACGACCA GGACACCATG GGCAAAGCCG ACTTCATGGG CCGGACCTTT 
GCCAAGCCGC TGGTGAAGAT GGCCGACGAG GCGTACTGCC CACCCCGCTT CCCGCCCCAG 
CTGGAGTACT ATCAGATCTA CCGGGGCAAT GCCACGGCCG GGGACCTGCT GGCTGCCTTC 
GAGCTGCTGC AGATTGGACC AGCAGGGAAG GCCGACCTGC CGCCGATCAA CGGCCCAGTG 
GACATGGACC GAGGTCCCAT CATGCCTGTG CCTGTGGGCA TCCGACCCGT GCTTAGCAAG 
TACCGGGTGG AGGTGCTGTT CTGGGGCCTG CGAGACCTGA AGCGGGTAAA CCTGGCCCAG 
GTGGACAGGC CGCGGGTGGA CATCGAGTGC GCGGGGAAGG GGGTGCAGTC CTCCCTGATC 
CACAATTACA AGAAGAACCC CAACTTCAGC ACCCTGGTCA AGTGGTTTGA AGTGGACCTC 
CCAGAGAACG AGCTCCTGCA CCCGCCCTTG AACATCCGCG TGGTGGACTG CCGGGCCTTC 
GGCCGCTACA CCCTGGTCGG CTCCCACGCT GTCAGCTCCC TGCGGCGCTT CATCTACCGA 
CCCCCGGACC GCTCAGCTCC CAACTGGAAC ACTTCGGGGG AGGTCGTGGT GAGCATGGAG 
CCGGAGGTGC CCATCAAGAA GCTGGAGACC ATGGTGAAGC TGGACGCGAC TTCCGATGCC 
GTCATCAAGG TGGATGTGGC TGAGGAAGAG AAGGAGAGGA AGAAGAAGAA GAAGAAGGGC 
AGCTCAGATG AGCCGGAGGA GGAGGAGCCA GATGAGAGTA TGTTGGACTG GTGGTCCAAG 
TACTTTGCCT CCATTGACAC CATGAAAGAG CAAATTCGAC AACAAGAAAT CTCAGGAATT 
GACCTGGAGG AGAAGGAGGA GATGGACAAC ACTGAAGGCC TGAAGGGGCC GATGAAGGGC 
AAGGAGAAGG CAAGGGCTGC AAAGGAGGAG AAGAAGAAAA GAACCCAGAG CCTAGGCCCT 
GGCCAGGGGT CGGAGGCCCC CGAGAAGAGG AAACCCAAGA TTGATGAGCT CAAGGTGTAC 
CCCAAGGAGC TGGAGTCAGA GTTCGACAAC TTCGAGGACT GGCTGCACAC GTTCAACCTG 
CTTCGGGGGA AGACGGGGGA CGACGAGGAT GGCGGGACCG AGGAGGAGCG CATTGTGGGC 
CGGTTCAAGG GCTCCCTCTG CGTGTACAAA GTGCCACTCC CAGAGGACGT GTCCCGGGAA 
GCCGGCTACG ACCCCACCTA CGGCATGTTC CAGGGCATCC CCAGCAATGA CCCCATCAAC 
GTGCTGGTCC GGGTCTACGT GGTCCGGGCC ACGGACCTGC ACCCCGCAGA CATCAACGGC 
AAAGCTGACC CGTACATCGC CATCCGGCTA GGCAAGACTG ATATCCGTGA CAAGGAGAAC 
TACATCTCCA AGCAGCTCAA CCCCGTCTTT GGGAAGTCCT TTGACATCGA AGCCTGCTTC 
CCCATGGAAT CCATGCTGAC GGTGGCCGTG TACGACTGGG ATCTGGTAGG CACTGATGAC 
CTCATTGGGG AAACCAAGAT CGACCTGGAA AACCGCTTCT ACAGCAAGCA CCGCGCCACC 
TGCGGCATCG CCCAGACCTA CTCCGTACAC GGCTACAATA TCTGGCGGGA CCCTATGAAG 
CCCAGCCAGA TCCTGACCCG CCTCTGCAAG GAGGGCAAAG TGGACGGCCC CCACTTCGGG 
CCCCCCGGGA GAGTGAAGGT GTCCAACCGC ATCTTCACAG GGCCCTCTGA GATCGAGGAC 
GAGAATGGCC AGAGGAAGCC CACAGAAGAG CACGTGGCAC TGCTGGCCCT GAGACACTGG 
GAGGACATCC CCCGCGTGGG CTGTCGCCTG GTGCCCGAAC ATGTGGAGAC ACGGCCGCTG 
CTCAACCCCG ACAAGCCGGG CATCGAACAG GGCCGCCTGG AGCTGTGGGT GGACATGTTT 
CCCATGGACA TGCCGTCCCC CGGGACACCT CTGGACATCT CCCCTCGGAA GCCCAAGAAG 
TACGAGCTGA GGGTCATCGT GTGGAACACG GATGAGGTGG TCTTGGAAGA TGACGACTTC 
TTCACTGGGG AGAAGTCCAG CGACATTTTC GTGCGGGGGT GGCTGAAGGG CCAGCAGGAG 
GACAAGCAGG ACACAGACGT CCACTACCAC TCCCTCACGG GCGAGGGCAA CTTCAACTGG 
CGCTACCTGT TCCCCTTCGA CTACCTGGCT GCCGAGGAGA AGATAGTCAT CTCCAAGAAG 
GAGTCCATGT TCTCCTGGGA CGAGACCGAA TACAAGATCC CCGCACGGCT CACCCTGCAG 
ATCTGGGACG CCGACCACTT CTCTGCCGAT GACTTCCTGG GGGCCATCGA GCTGGACCTG 
AATCGCTTCC CTCGGGGCGC GAAAACGGCC AAGCAGTGCA CCATGGAGAT GGCCACAGGG 
GAGGTGGATG TGCCCCTGGT TTCCATCTTC AAGCAGAAGC GTGTCAAAGG CTGGTGGCCC 
CTCCTAGCCC GCAACGAGAA TGACGAGTTT GAGCTCACGG GCAAAGTGGA AGCAGAGTTG 
CATCTACTCA CCGCCGAGGA GGCGGAAAAG AATCCCGTGG GCCTGGCCCG CAATGAACCT 
GATCCCCTAG AGAAACCCAA CCGGCCCGAC ACCGCCTTCG TCTGGTTCCT CAACCCCCTC 
AAGTCCATCA AGTACCTCAT CTGCACGCGG TACAAGTGGC TCATCATCAA GATCGTGTTG 
GCGCTGCTGG GACTGCTCAT GCTGGCGCTC TTCCTCTACA GCCTCCCCGG TTACATGGTC 
AAGAAGCTCT TGGGGGCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOF ( XM_015473546.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329032.1 Bos taurus otoferlin (OTOF), transcript variant X2, mRNA.

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CloneID	OBa91932
Clone ID Related Accession (Same CDS sequence)	XM_015473546.1 , XM_015473546.2
Accession Version	XM_015473546.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	otoferlin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015473546.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTGC TCGTCCACCT CAAGACAGTC TCCGAGCTGC GGGGCAAGGG TGACCGGATC 
GCCAAAGTGA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TCCTGGAGAG CTGTGAGGAT 
GTGGCTGACT TCGATGAGAC CTTCCGGTGG CCGGTGGCCA GCAGCATCGA CAGCAATGAG 
ATGCTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT GATCGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGAC CGACACGCTG 
ATGGATGACA ACAATGCAAT CATCAAGACC AGCCTGTGCG TGGAGGTCCG GTATCAGGCC 
ACAGATGGCA CAGTGGGCTC CTGGGATGAC GGGGACTTTC TGGGAGAGGA GTCCCTTCAC 
GAGGAAGAGA AGGACAGCCA GGAGACGGAT GGGCTGCTAC CCGGCTCCCG TCCCAGCTCC 
CGGCCATCAG GCGAGAAGAG TTTCCGGAGA GCCGGGAGAA GCGTGTTCTC CGCCATGAAG 
CTCGGCAAGA ACCGGCCTCA CAAGGAGGAA CCCCAAAGAC AAGATGAACC AGCTGTTCTG 
GAGATGGAGG ACCTTGACCG CCTGGCCATT CGGCTGGGGG ATGGGCTGGA CCCTGACTCC 
GTGTCGCTAG CATCCGTCAC AGCCCTCACC ACCAATGTCT CCAACAAGCG GTCTAAGCCA 
GACATCAAGA TGGAGCCAAG TGCCGGGCGG CCAATGGACT ACCAGGTCAG CGTCACAGTC 
ATCGAGGCTC GGCAGCTGGT GGGCTTGAAC ATGGACCCCG TGGTGTGTGT GGAGGTGGGC 
GATGAGAAGA AGTACACCTC CATGAAGGAG TCCACTAACT GCCCCTACTA TAATGAGTAC 
TTCGTCTTTG ACTTCCACGT CTCTCCTGAT GTCATGTTTG ACAAGATCAT CAAGATCTCG 
GTGATTCACT CCAAGAACCT GCTGCGCAGT GGCACCCTGG TGGGGTCCTT CAAAATGGAC 
GTGGGAACCG TGTACTCCCA GCCAGAGCAC CAGTTCCACC ACAAGTGGGC CGTCCTGTCC 
GACCCTGACG ACATCTCCGC GGGACTGAAG GGCTACGTGA AGTGCGATGT CGCTGTGGTG 
GGCAAGGGCG ACAACATTAA GACTCCCCAC AAGGCCAATG AGACGGATGA GGATGACATT 
GAGGGGAACT TGCTGCTCCC CGAAGGGGTG CCCCCTGAAC GCCAGTGGGC CCGGTTCTAC 
GTGAAGATCT ACCGAGCAGA GGGGCTGCCC CGCATGAACA CGAGCCTCAT GGCCAATGTG 
AAGAAGGCTT TCACTGGCGA GAACAAGGAT CTGGTCGACC CCTATGTGCA AGTCTTCTTT 
GCCGGCCAGA AGGGCAAGAC TTCAGTGCAG AAAAGCAGCT ACGAGCCACT GTGGAATGAG 
CAGGTGGTCT TCACAGACCT GTTTCCCCCA CTCTGCAAGC GCATGAAGGT GCAAATCCGG 
GACTCGGACA AGGTCAATGA CGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT 
TCCAACGATG GAGACAAAGG CTTCCTGCCC ACGCTGGGCC CTGCCTGGGT GAACATGTAC 
GGCTCCACAC GCAATTACAC GCTGCTAGAC GAGCATCAGG ACCTGAACGA GGGCCTGGGG 
GAGGGTGTGT CCTTCCGTGC CCGCCTCATG CTGGGCCTGG CGGTGGAGAT CCTGGACACC 
TCCAACCCTG AGATCACCAG CTCCACTGAG GTGCAGGTGG AGCAGGCCAC GCCCATCTCC 
GAGAGCTGCA CGGGGAAAAT AGAAGAATTC TTTCTATTTG GAGCCTTCCT GGAGGCCTCA 
ATGATCGACC GGAAAAATGG AGACAAGCCC ATCACCTTTG AGGTCACCGT AGGCAACTAC 
GGGAACGAAG TCGATGGGCT GTCACGACCC CATCGGCCTC GGCCTCGGAA GGAGCCTGGG 
GATGAGGAGG AAGTCGATCT GATCCAGAAC TTGAGTGATG AAGAGACTGA CGAGGGTGGG 
GGCCTGGCCT CGGTCTCCTC CACGCCACCC ATGCGGCCCC AGATCACTGA CAGGAACTAC 
TTCCATCTGC CCTACCTGGA GCACAAACCC TGCATCTACA TCAAGAGCTG GTGGCCTGAC 
CAACGCCGTC GCCTGTACAA TGCCAACATC ATGGACCACA TCGCAGACAA GCTGGAAGAA 
GGCCTGAATG ATGTGCAGGA GATGATCAAA ACGGAGAAGT CGTACCCTGA GCGTCGACTT 
CGGGGCGTCC TAGAGGAGCT GAGCTCTGGT TGCTACCGCT TCCTCTCCCT CGCGGACAAG 
GACCAGGGCC ACTCATCCCG CACCAGGCTG GACCGGGAGC GTCTCAAGTC CTGCATGCGG 
GAGCTGGAAA GCATGGGGCA GCAGGCCAAG ACCCTGCGGG CACAAGTGAA GCGGCACACG 
GTGCGCGACA AGCTGAGGCT CTGTCAGAAC TTCCTGCAGA AGCTGCGCTT CCTGGCAGAC 
GAGCCCCAGC ACAGCATCCC TGACGTCTTC ATCTGGATGA TGAGCAACAA CAAGCGCATT 
GCCTACACCC GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTGGAGGA GGAACTGGGC 
AAGGACTGTG CCAAGGTCAA GACACTCTTC CTCAAGGTGC CGGAAGGGGA ATGGATTAGC 
AGCGGGTGGA GGGGCTGGGG GCTGGGCTAC TCTCAAGAGG TCATCTTCCT CTTGGAGGGA 
GCAGCATCCA CACCCCTGGG TCTGCCAGGC CTGAGTGGAC AAGATGCTCA GGGAAGAGCT 
AAAGAGAATA GGAAGGGGGA TCCCGCCCTG CCCATCCCCT TCTGTGCTGT GTTTCCCCAC 
TGCACAGCCC AGTGGGATGG CTGCGGTGGG GATGGGCCCC CTCTGACCAC AGCTTCTTCA 
GCTCCCTGCC CCTTCCTCAC CCCGAGGGCT CTGGCAGCTG ACCCTGAGCC CACCCCCCAG 
CTGCCAGGGA AGCGGGGCTT CGGCTCGGCA GGCTGGACAG TGCAGGCCAA GCTGGAGCTC 
TACCTGTGGC TGGGTCTAAG CAAGCAGCGC AAGGACTTCC TGTGTGGCCT GCCCTGCGGC 
TTCGAGGAGG TCAAGGCAGC CCAGGGCCTG GGCCTACTCT CCTTCCCGCC CATCAGCCTG 
GTCTACACTA AGAAGCAGGC CTTCCAGCTC CGCGCCCACA TGTACCAGGC TCGCAGCCTC 
TTTGCTGCTG ACAGCACCGG CCTCTCAGAC CCCTTTGCCC GTGTCTTCTT CATCAACCAG 
AGTCAGTGCA CAGAGGTACT GAATGAGACC CTGTGCCCCA CCTGGGACCA GATGCTGGTG 
TTCGACAACC TGGAGCTCTA CGGTGAAGCT CATGAGCTGC GGGACGATCC CCCCATCATT 
GTTATCGAAA TCTACGACCA GGACACCATG GGCAAAGCCG ACTTCATGGG CCGGACCTTT 
GCCAAGCCGC TGGTGAAGAT GGCCGACGAG GCGTACTGCC CACCCCGCTT CCCGCCCCAG 
CTGGAGTACT ATCAGATCTA CCGGGGCAAT GCCACGGCCG GGGACCTGCT GGCTGCCTTC 
GAGCTGCTGC AGATTGGACC AGCAGGGAAG GCCGACCTGC CGCCGATCAA CGGCCCAGTG 
GACATGGACC GAGGTCCCAT CATGCCTGTG CCTGTGGGCA TCCGACCCGT GCTTAGCAAG 
TACCGGGTGG AGGTGCTGTT CTGGGGCCTG CGAGACCTGA AGCGGGTAAA CCTGGCCCAG 
GTGGACAGGC CGCGGGTGGA CATCGAGTGC GCGGGGAAGG GGGTGCAGTC CTCCCTGATC 
CACAATTACA AGAAGAACCC CAACTTCAGC ACCCTGGTCA AGTGGTTTGA AGTGGACCTC 
CCAGAGAACG AGCTCCTGCA CCCGCCCTTG AACATCCGCG TGGTGGACTG CCGGGCCTTC 
GGCCGCTACA CCCTGGTCGG CTCCCACGCT GTCAGCTCCC TGCGGCGCTT CATCTACCGA 
CCCCCGGACC GCTCAGCTCC CAACTGGAAC ACTTCGGGGG AGGTCGTGGT GAGCATGGAG 
CCGGAGGTGC CCATCAAGAA GCTGGAGACC ATGGTGAAGC TGGACGCGAC TTCCGATGCC 
GTCATCAAGG TGGATGTGGC TGAGGAAGAG AAGGAGAGGA AGAAGAAGAA GAAGAAGGGC 
AGCTCAGATG AGCCGGAGGA GGAGGAGCCA GATGAGAGTA TGTTGGACTG GTGGTCCAAG 
TACTTTGCCT CCATTGACAC CATGAAAGAG CAAATTCGAC AACAAGAAAT CTCAGGAATT 
GACCTGGAGG AGAAGGAGGA GATGGACAAC ACTGAAGGCC TGAAGGGGCC GATGAAGGGC 
AAGGAGAAGG CAAGGGCTGC AAAGGAGGAG AAGAAGAAAA GAACCCAGAG CCTAGGCCCT 
GGCCAGGGGT CGGAGGCCCC CGAGAAGAGG AAACCCAAGA TTGATGAGCT CAAGGTGTAC 
CCCAAGGAGC TGGAGTCAGA GTTCGACAAC TTCGAGGACT GGCTGCACAC GTTCAACCTG 
CTTCGGGGGA AGACGGGGGA CGACGAGGAT GGCGGGACCG AGGAGGAGCG CATTGTGGGC 
CGGTTCAAGG GCTCCCTCTG CGTGTACAAA GTGCCACTCC CAGAGGACGT GTCCCGGGAA 
GCCGGCTACG ACCCCACCTA CGGCATGTTC CAGGGCATCC CCAGCAATGA CCCCATCAAC 
GTGCTGGTCC GGGTCTACGT GGTCCGGGCC ACGGACCTGC ACCCCGCAGA CATCAACGGC 
AAAGCTGACC CGTACATCGC CATCCGGCTA GGCAAGACTG ATATCCGTGA CAAGGAGAAC 
TACATCTCCA AGCAGCTCAA CCCCGTCTTT GGGAAGTCCT TTGACATCGA AGCCTGCTTC 
CCCATGGAAT CCATGCTGAC GGTGGCCGTG TACGACTGGG ATCTGGTAGG CACTGATGAC 
CTCATTGGGG AAACCAAGAT CGACCTGGAA AACCGCTTCT ACAGCAAGCA CCGCGCCACC 
TGCGGCATCG CCCAGACCTA CTCCGTACAC GGCTACAATA TCTGGCGGGA CCCTATGAAG 
CCCAGCCAGA TCCTGACCCG CCTCTGCAAG GAGGGCAAAG TGGACGGCCC CCACTTCGGG 
CCCCCCGGGA GAGTGAAGGT GTCCAACCGC ATCTTCACAG GGCCCTCTGA GATCGAGGAC 
GAGAATGGCC AGAGGAAGCC CACAGAAGAG CACGTGGCAC TGCTGGCCCT GAGACACTGG 
GAGGACATCC CCCGCGTGGG CTGTCGCCTG GTGCCCGAAC ATGTGGAGAC ACGGCCGCTG 
CTCAACCCCG ACAAGCCGGG CATCGAACAG GGCCGCCTGG AGCTGTGGGT GGACATGTTT 
CCCATGGACA TGCCGTCCCC CGGGACACCT CTGGACATCT CCCCTCGGAA GCCCAAGAAG 
TACGAGCTGA GGGTCATCGT GTGGAACACG GATGAGGTGG TCTTGGAAGA TGACGACTTC 
TTCACTGGGG AGAAGTCCAG CGACATTTTC GTGCGGGGGT GGCTGAAGGG CCAGCAGGAG 
GACAAGCAGG ACACAGACGT CCACTACCAC TCCCTCACGG GCGAGGGCAA CTTCAACTGG 
CGCTACCTGT TCCCCTTCGA CTACCTGGCT GCCGAGGAGA AGATAGTCAT CTCCAAGAAG 
GAGTCCATGT TCTCCTGGGA CGAGACCGAA TACAAGATCC CCGCACGGCT CACCCTGCAG 
ATCTGGGACG CCGACCACTT CTCTGCCGAT GACTTCCTGG GGGCCATCGA GCTGGACCTG 
AATCGCTTCC CTCGGGGCGC GAAAACGGCC AAGCAGTGCA CCATGGAGAT GGCCACAGGG 
GAGGTGGATG TGCCCCTGGT TTCCATCTTC AAGCAGAAGC GTGTCAAAGG CTGGTGGCCC 
CTCCTAGCCC GCAACGAGAA TGACGAGTTT GAGCTCACGG GCAAAGTGGA AGCAGAGTTG 
CATCTACTCA CCGCCGAGGA GGCGGAAAAG AATCCCGTGG GCCTGGCCCG CAATGAACCT 
GATCCCCTAG AGAAACCCAA CCGGCCCGAC ACCGCCTTCG TCTGGTTCCT CAACCCCCTC 
AAGTCCATCA AGTACCTCAT CTGCACGCGG TACAAGTGGC TCATCATCAA GATCGTGTTG 
GCGCTGCTGG GACTGCTCAT GCTGGCGCTC TTCCTCTACA GCCTCCCCGG TTACATGGTC 
AAGAAGCTCT TGGGGGCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329032.1
CDS	181..6441
Translation	MALLVHLKTVSELRGKGDRIAKVTFRGQSFYSRVLESCEDVADFDETFRWPVASSIDSNEMLEIQIFNYSKVFSNKLIGTFRMVLQKVVEENHVEVTDTLMDDNNAIIKTSLCVEVRYQATDGTVGSWDDGDFLGEESLHEEEKDSQETDGLLPGSRPSSRPSGEKSFRRAGRSVFSAMKLGKNRPHKEEPQRQDEPAVLEMEDLDRLAIRLGDGLDPDSVSLASVTALTTNVSNKRSKPDIKMEPSAGRPMDYQVSVTVIEARQLVGLNMDPVVCVEVGDEKKYTSMKESTNCPYYNEYFVFDFHVSPDVMFDKIIKISVIHSKNLLRSGTLVGSFKMDVGTVYSQPEHQFHHKWAVLSDPDDISAGLKGYVKCDVAVVGKGDNIKTPHKANETDEDDIEGNLLLPEGVPPERQWARFYVKIYRAEGLPRMNTSLMANVKKAFTGENKDLVDPYVQVFFAGQKGKTSVQKSSYEPLWNEQVVFTDLFPPLCKRMKVQIRDSDKVNDVAIGTHFIDLRKISNDGDKGFLPTLGPAWVNMYGSTRNYTLLDEHQDLNEGLGEGVSFRARLMLGLAVEILDTSNPEITSSTEVQVEQATPISESCTGKIEEFFLFGAFLEASMIDRKNGDKPITFEVTVGNYGNEVDGLSRPHRPRPRKEPGDEEEVDLIQNLSDEETDEGGGLASVSSTPPMRPQITDRNYFHLPYLEHKPCIYIKSWWPDQRRRLYNANIMDHIADKLEEGLNDVQEMIKTEKSYPERRLRGVLEELSSGCYRFLSLADKDQGHSSRTRLDRERLKSCMRELESMGQQAKTLRAQVKRHTVRDKLRLCQNFLQKLRFLADEPQHSIPDVFIWMMSNNKRIAYTRVPSKDLLFSIVEEELGKDCAKVKTLFLKVPEGEWISSGWRGWGLGYSQEVIFLLEGAASTPLGLPGLSGQDAQGRAKENRKGDPALPIPFCAVFPHCTAQWDGCGGDGPPLTTASSAPCPFLTPRALAADPEPTPQLPGKRGFGSAGWTVQAKLELYLWLGLSKQRKDFLCGLPCGFEEVKAAQGLGLLSFPPISLVYTKKQAFQLRAHMYQARSLFAADSTGLSDPFARVFFINQSQCTEVLNETLCPTWDQMLVFDNLELYGEAHELRDDPPIIVIEIYDQDTMGKADFMGRTFAKPLVKMADEAYCPPRFPPQLEYYQIYRGNATAGDLLAAFELLQIGPAGKADLPPINGPVDMDRGPIMPVPVGIRPVLSKYRVEVLFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSSLIHNYKKNPNFSTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHAVSSLRRFIYRPPDRSAPNWNTSGEVVVSMEPEVPIKKLETMVKLDATSDAVIKVDVAEEEKERKKKKKKGSSDEPEEEEPDESMLDWWSKYFASIDTMKEQIRQQEISGIDLEEKEEMDNTEGLKGPMKGKEKARAAKEEKKKRTQSLGPGQGSEAPEKRKPKIDELKVYPKELESEFDNFEDWLHTFNLLRGKTGDDEDGGTEEERIVGRFKGSLCVYKVPLPEDVSREAGYDPTYGMFQGIPSNDPINVLVRVYVVRATDLHPADINGKADPYIAIRLGKTDIRDKENYISKQLNPVFGKSFDIEACFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRFYSKHRATCGIAQTYSVHGYNIWRDPMKPSQILTRLCKEGKVDGPHFGPPGRVKVSNRIFTGPSEIEDENGQRKPTEEHVALLALRHWEDIPRVGCRLVPEHVETRPLLNPDKPGIEQGRLELWVDMFPMDMPSPGTPLDISPRKPKKYELRVIVWNTDEVVLEDDDFFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYLFPFDYLAAEEKIVISKKESMFSWDETEYKIPARLTLQIWDADHFSADDFLGAIELDLNRFPRGAKTAKQCTMEMATGEVDVPLVSIFKQKRVKGWWPLLARNENDEFELTGKVEAELHLLTAEEAEKNPVGLARNEPDPLEKPNRPDTAFVWFLNPLKSIKYLICTRYKWLIIKIVLALLGLLMLALFLYSLPGYMVKKLLGA

Target ORF information:

RefSeq Version	XM_015473546.2
Organism	Bos taurus(cattle)
Definition	Bos taurus otoferlin (OTOF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473546.2

ORF Insert Sequence:

ATGGCCCTGC TCGTCCACCT CAAGACAGTC TCCGAGCTGC GGGGCAAGGG TGACCGGATC 
GCCAAAGTGA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TCCTGGAGAG CTGTGAGGAT 
GTGGCTGACT TCGATGAGAC CTTCCGGTGG CCGGTGGCCA GCAGCATCGA CAGCAATGAG 
ATGCTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT GATCGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGAC CGACACGCTG 
ATGGATGACA ACAATGCAAT CATCAAGACC AGCCTGTGCG TGGAGGTCCG GTATCAGGCC 
ACAGATGGCA CAGTGGGCTC CTGGGATGAC GGGGACTTTC TGGGAGAGGA GTCCCTTCAC 
GAGGAAGAGA AGGACAGCCA GGAGACGGAT GGGCTGCTAC CCGGCTCCCG TCCCAGCTCC 
CGGCCATCAG GCGAGAAGAG TTTCCGGAGA GCCGGGAGAA GCGTGTTCTC CGCCATGAAG 
CTCGGCAAGA ACCGGCCTCA CAAGGAGGAA CCCCAAAGAC AAGATGAACC AGCTGTTCTG 
GAGATGGAGG ACCTTGACCG CCTGGCCATT CGGCTGGGGG ATGGGCTGGA CCCTGACTCC 
GTGTCGCTAG CATCCGTCAC AGCCCTCACC ACCAATGTCT CCAACAAGCG GTCTAAGCCA 
GACATCAAGA TGGAGCCAAG TGCCGGGCGG CCAATGGACT ACCAGGTCAG CGTCACAGTC 
ATCGAGGCTC GGCAGCTGGT GGGCTTGAAC ATGGACCCCG TGGTGTGTGT GGAGGTGGGC 
GATGAGAAGA AGTACACCTC CATGAAGGAG TCCACTAACT GCCCCTACTA TAATGAGTAC 
TTCGTCTTTG ACTTCCACGT CTCTCCTGAT GTCATGTTTG ACAAGATCAT CAAGATCTCG 
GTGATTCACT CCAAGAACCT GCTGCGCAGT GGCACCCTGG TGGGGTCCTT CAAAATGGAC 
GTGGGAACCG TGTACTCCCA GCCAGAGCAC CAGTTCCACC ACAAGTGGGC CGTCCTGTCC 
GACCCTGACG ACATCTCCGC GGGACTGAAG GGCTACGTGA AGTGCGATGT CGCTGTGGTG 
GGCAAGGGCG ACAACATTAA GACTCCCCAC AAGGCCAATG AGACGGATGA GGATGACATT 
GAGGGGAACT TGCTGCTCCC CGAAGGGGTG CCCCCTGAAC GCCAGTGGGC CCGGTTCTAC 
GTGAAGATCT ACCGAGCAGA GGGGCTGCCC CGCATGAACA CGAGCCTCAT GGCCAATGTG 
AAGAAGGCTT TCACTGGCGA GAACAAGGAT CTGGTCGACC CCTATGTGCA AGTCTTCTTT 
GCCGGCCAGA AGGGCAAGAC TTCAGTGCAG AAAAGCAGCT ACGAGCCACT GTGGAATGAG 
CAGGTGGTCT TCACAGACCT GTTTCCCCCA CTCTGCAAGC GCATGAAGGT GCAAATCCGG 
GACTCGGACA AGGTCAATGA CGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT 
TCCAACGATG GAGACAAAGG CTTCCTGCCC ACGCTGGGCC CTGCCTGGGT GAACATGTAC 
GGCTCCACAC GCAATTACAC GCTGCTAGAC GAGCATCAGG ACCTGAACGA GGGCCTGGGG 
GAGGGTGTGT CCTTCCGTGC CCGCCTCATG CTGGGCCTGG CGGTGGAGAT CCTGGACACC 
TCCAACCCTG AGATCACCAG CTCCACTGAG GTGCAGGTGG AGCAGGCCAC GCCCATCTCC 
GAGAGCTGCA CGGGGAAAAT AGAAGAATTC TTTCTATTTG GAGCCTTCCT GGAGGCCTCA 
ATGATCGACC GGAAAAATGG AGACAAGCCC ATCACCTTTG AGGTCACCGT AGGCAACTAC 
GGGAACGAAG TCGATGGGCT GTCACGACCC CATCGGCCTC GGCCTCGGAA GGAGCCTGGG 
GATGAGGAGG AAGTCGATCT GATCCAGAAC TTGAGTGATG AAGAGACTGA CGAGGGTGGG 
GGCCTGGCCT CGGTCTCCTC CACGCCACCC ATGCGGCCCC AGATCACTGA CAGGAACTAC 
TTCCATCTGC CCTACCTGGA GCACAAACCC TGCATCTACA TCAAGAGCTG GTGGCCTGAC 
CAACGCCGTC GCCTGTACAA TGCCAACATC ATGGACCACA TCGCAGACAA GCTGGAAGAA 
GGCCTGAATG ATGTGCAGGA GATGATCAAA ACGGAGAAGT CGTACCCTGA GCGTCGACTT 
CGGGGCGTCC TAGAGGAGCT GAGCTCTGGT TGCTACCGCT TCCTCTCCCT CGCGGACAAG 
GACCAGGGCC ACTCATCCCG CACCAGGCTG GACCGGGAGC GTCTCAAGTC CTGCATGCGG 
GAGCTGGAAA GCATGGGGCA GCAGGCCAAG ACCCTGCGGG CACAAGTGAA GCGGCACACG 
GTGCGCGACA AGCTGAGGCT CTGTCAGAAC TTCCTGCAGA AGCTGCGCTT CCTGGCAGAC 
GAGCCCCAGC ACAGCATCCC TGACGTCTTC ATCTGGATGA TGAGCAACAA CAAGCGCATT 
GCCTACACCC GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTGGAGGA GGAACTGGGC 
AAGGACTGTG CCAAGGTCAA GACACTCTTC CTCAAGGTGC CGGAAGGGGA ATGGATTAGC 
AGCGGGTGGA GGGGCTGGGG GCTGGGCTAC TCTCAAGAGG TCATCTTCCT CTTGGAGGGA 
GCAGCATCCA CACCCCTGGG TCTGCCAGGC CTGAGTGGAC AAGATGCTCA GGGAAGAGCT 
AAAGAGAATA GGAAGGGGGA TCCCGCCCTG CCCATCCCCT TCTGTGCTGT GTTTCCCCAC 
TGCACAGCCC AGTGGGATGG CTGCGGTGGG GATGGGCCCC CTCTGACCAC AGCTTCTTCA 
GCTCCCTGCC CCTTCCTCAC CCCGAGGGCT CTGGCAGCTG ACCCTGAGCC CACCCCCCAG 
CTGCCAGGGA AGCGGGGCTT CGGCTCGGCA GGCTGGACAG TGCAGGCCAA GCTGGAGCTC 
TACCTGTGGC TGGGTCTAAG CAAGCAGCGC AAGGACTTCC TGTGTGGCCT GCCCTGCGGC 
TTCGAGGAGG TCAAGGCAGC CCAGGGCCTG GGCCTACTCT CCTTCCCGCC CATCAGCCTG 
GTCTACACTA AGAAGCAGGC CTTCCAGCTC CGCGCCCACA TGTACCAGGC TCGCAGCCTC 
TTTGCTGCTG ACAGCACCGG CCTCTCAGAC CCCTTTGCCC GTGTCTTCTT CATCAACCAG 
AGTCAGTGCA CAGAGGTACT GAATGAGACC CTGTGCCCCA CCTGGGACCA GATGCTGGTG 
TTCGACAACC TGGAGCTCTA CGGTGAAGCT CATGAGCTGC GGGACGATCC CCCCATCATT 
GTTATCGAAA TCTACGACCA GGACACCATG GGCAAAGCCG ACTTCATGGG CCGGACCTTT 
GCCAAGCCGC TGGTGAAGAT GGCCGACGAG GCGTACTGCC CACCCCGCTT CCCGCCCCAG 
CTGGAGTACT ATCAGATCTA CCGGGGCAAT GCCACGGCCG GGGACCTGCT GGCTGCCTTC 
GAGCTGCTGC AGATTGGACC AGCAGGGAAG GCCGACCTGC CGCCGATCAA CGGCCCAGTG 
GACATGGACC GAGGTCCCAT CATGCCTGTG CCTGTGGGCA TCCGACCCGT GCTTAGCAAG 
TACCGGGTGG AGGTGCTGTT CTGGGGCCTG CGAGACCTGA AGCGGGTAAA CCTGGCCCAG 
GTGGACAGGC CGCGGGTGGA CATCGAGTGC GCGGGGAAGG GGGTGCAGTC CTCCCTGATC 
CACAATTACA AGAAGAACCC CAACTTCAGC ACCCTGGTCA AGTGGTTTGA AGTGGACCTC 
CCAGAGAACG AGCTCCTGCA CCCGCCCTTG AACATCCGCG TGGTGGACTG CCGGGCCTTC 
GGCCGCTACA CCCTGGTCGG CTCCCACGCT GTCAGCTCCC TGCGGCGCTT CATCTACCGA 
CCCCCGGACC GCTCAGCTCC CAACTGGAAC ACTTCGGGGG AGGTCGTGGT GAGCATGGAG 
CCGGAGGTGC CCATCAAGAA GCTGGAGACC ATGGTGAAGC TGGACGCGAC TTCCGATGCC 
GTCATCAAGG TGGATGTGGC TGAGGAAGAG AAGGAGAGGA AGAAGAAGAA GAAGAAGGGC 
AGCTCAGATG AGCCGGAGGA GGAGGAGCCA GATGAGAGTA TGTTGGACTG GTGGTCCAAG 
TACTTTGCCT CCATTGACAC CATGAAAGAG CAAATTCGAC AACAAGAAAT CTCAGGAATT 
GACCTGGAGG AGAAGGAGGA GATGGACAAC ACTGAAGGCC TGAAGGGGCC GATGAAGGGC 
AAGGAGAAGG CAAGGGCTGC AAAGGAGGAG AAGAAGAAAA GAACCCAGAG CCTAGGCCCT 
GGCCAGGGGT CGGAGGCCCC CGAGAAGAGG AAACCCAAGA TTGATGAGCT CAAGGTGTAC 
CCCAAGGAGC TGGAGTCAGA GTTCGACAAC TTCGAGGACT GGCTGCACAC GTTCAACCTG 
CTTCGGGGGA AGACGGGGGA CGACGAGGAT GGCGGGACCG AGGAGGAGCG CATTGTGGGC 
CGGTTCAAGG GCTCCCTCTG CGTGTACAAA GTGCCACTCC CAGAGGACGT GTCCCGGGAA 
GCCGGCTACG ACCCCACCTA CGGCATGTTC CAGGGCATCC CCAGCAATGA CCCCATCAAC 
GTGCTGGTCC GGGTCTACGT GGTCCGGGCC ACGGACCTGC ACCCCGCAGA CATCAACGGC 
AAAGCTGACC CGTACATCGC CATCCGGCTA GGCAAGACTG ATATCCGTGA CAAGGAGAAC 
TACATCTCCA AGCAGCTCAA CCCCGTCTTT GGGAAGTCCT TTGACATCGA AGCCTGCTTC 
CCCATGGAAT CCATGCTGAC GGTGGCCGTG TACGACTGGG ATCTGGTAGG CACTGATGAC 
CTCATTGGGG AAACCAAGAT CGACCTGGAA AACCGCTTCT ACAGCAAGCA CCGCGCCACC 
TGCGGCATCG CCCAGACCTA CTCCGTACAC GGCTACAATA TCTGGCGGGA CCCTATGAAG 
CCCAGCCAGA TCCTGACCCG CCTCTGCAAG GAGGGCAAAG TGGACGGCCC CCACTTCGGG 
CCCCCCGGGA GAGTGAAGGT GTCCAACCGC ATCTTCACAG GGCCCTCTGA GATCGAGGAC 
GAGAATGGCC AGAGGAAGCC CACAGAAGAG CACGTGGCAC TGCTGGCCCT GAGACACTGG 
GAGGACATCC CCCGCGTGGG CTGTCGCCTG GTGCCCGAAC ATGTGGAGAC ACGGCCGCTG 
CTCAACCCCG ACAAGCCGGG CATCGAACAG GGCCGCCTGG AGCTGTGGGT GGACATGTTT 
CCCATGGACA TGCCGTCCCC CGGGACACCT CTGGACATCT CCCCTCGGAA GCCCAAGAAG 
TACGAGCTGA GGGTCATCGT GTGGAACACG GATGAGGTGG TCTTGGAAGA TGACGACTTC 
TTCACTGGGG AGAAGTCCAG CGACATTTTC GTGCGGGGGT GGCTGAAGGG CCAGCAGGAG 
GACAAGCAGG ACACAGACGT CCACTACCAC TCCCTCACGG GCGAGGGCAA CTTCAACTGG 
CGCTACCTGT TCCCCTTCGA CTACCTGGCT GCCGAGGAGA AGATAGTCAT CTCCAAGAAG 
GAGTCCATGT TCTCCTGGGA CGAGACCGAA TACAAGATCC CCGCACGGCT CACCCTGCAG 
ATCTGGGACG CCGACCACTT CTCTGCCGAT GACTTCCTGG GGGCCATCGA GCTGGACCTG 
AATCGCTTCC CTCGGGGCGC GAAAACGGCC AAGCAGTGCA CCATGGAGAT GGCCACAGGG 
GAGGTGGATG TGCCCCTGGT TTCCATCTTC AAGCAGAAGC GTGTCAAAGG CTGGTGGCCC 
CTCCTAGCCC GCAACGAGAA TGACGAGTTT GAGCTCACGG GCAAAGTGGA AGCAGAGTTG 
CATCTACTCA CCGCCGAGGA GGCGGAAAAG AATCCCGTGG GCCTGGCCCG CAATGAACCT 
GATCCCCTAG AGAAACCCAA CCGGCCCGAC ACCGCCTTCG TCTGGTTCCT CAACCCCCTC 
AAGTCCATCA AGTACCTCAT CTGCACGCGG TACAAGTGGC TCATCATCAA GATCGTGTTG 
GCGCTGCTGG GACTGCTCAT GCTGGCGCTC TTCCTCTACA GCCTCCCCGG TTACATGGTC 
AAGAAGCTCT TGGGGGCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Marçais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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OTOF cDNA ORF clone, Bos taurus(cattle)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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