dnmt1.L cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	dnmt1.L
Entrez Gene ID	399257
Full Name	DNA methyltransferase 1 L homeolog
Synonyms	DNA MeTase,dnmt1,xDnmt1
General protein information	Preferred Names DNA methyltransferase 1 L homeolog Names DNA methyltransferase 1 L homeolog DNA (cytosine-5-)-methyltransferase 1 L homeolog
Gene Type	protein-coding
Organism	Xenopus laevis(African clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001090552.1	NP_001084021.1	DNA methyltransferase 1 L homeolog

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Lsh Is Essential for Maintaining Global DNA Methylation Levels in Amphibia and Fish and Interacts Directly with Dnmt1.
Dunican DS, Pennings S, Meehan RR
BioMed research international2015740637(2015)

MBD4 and MLH1 are required for apoptotic induction in xDNMT1-depleted embryos.
Ruzov A, Shorning B, Mortusewicz O, Dunican DS, Leonhardt H, Meehan RR
Development (Cambridge, England)136(13)2277-86(2009 Jul)

xDnmt1 regulates transcriptional silencing in pre-MBT Xenopus embryos independently of its catalytic function.
Dunican DS, Ruzov A, Hackett JA, Meehan RR
Development (Cambridge, England)135(7)1295-302(2008 Apr)

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

Isolation and expression of a Xenopus laevis DNA methyltransferase cDNA.
Kimura H, Ishihara G, Tajima S
Journal of biochemistry120(6)1182-9(1996 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following dnmt1.L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dnmt1.L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAf08701	NM_001090552.1 Latest version!	Xenopus laevis DNA methyltransferase 1 L homeolog (dnmt1.L), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dnmt1.L ( NM_001090552.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> NP_001084021.1 Xenopus laevis DNA methyltransferase 1 L homeolog (dnmt1.L), mRNA.

Price & Availability↑

CloneID	OAf08701
Clone ID Related Accession (Same CDS sequence)	NM_001090552.1
Accession Version	NM_001090552.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1488297600000
Organism	Xenopus laevis(African clawed frog)
Product	DNA methyltransferase 1 L homeolog
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from D78638.1. On Jul 25, 2007 this sequence version replaced NM_001090553.1. ##Evidence-Data-START## Transcript exon combination :: D78638.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00012418, SAMD00012419 [ECO:0000350] ##Evidence-Data-END##

ATGCCGGCCC AGTCCACTTC CTTGGCTCTG CCTGCTGATG TCAGGAAACG GCTAAAGGAT 
TTGAAGAGGG ATCAGGATGG AATGACTGAG AAGGAACATG TACAGCAGAA ACTGAGTTTG 
GTGCTTGGCT TCCTTGAAGC AGATGCTAGA AACAAACTGA ATGATCTCGA GAGCAAGTTG 
AGCAGTGAAG AACTGTCTGA GGAAGGATAC CTTACAAAAG TGAAGTCTCT TCTGGGGAAG 
CAGTTGAGCT TTGAAAACGT AGACCTTGCT TTGAACGGAG AAACAAATGG CTGCTCCACC 
AATGGTACCT GCGGCAGTGA TGAGGAAGAT GTACAGTTGT CGGAGTCTAA TACTTCTGGT 
GTTAAAAACC GCAAACCTAG GAAAAGTAAA GTTAATGGAG AAAACAAGAA ATCACCAGCC 
CGTGCCCGCC CATCAAGAAG CACTGCAGGC AAACAACCCA CCATCCTCTC CATGTTCTCT 
AAAGGGTCAA CCAAGCGGAA ATCCAGTGAT GATGAGAAAG ATACAGATGT TCCAGCTGAT 
GCAGACCAAC CAGAAGAGAA GGAAAAAGAA GAGAAAAGAA TAAAAATCGA AGTTAATGAA 
AGCGAGGACA AACGGTCTGA TGCGGAAGAA GGTAAAAAGG CAAAACCTGT TCAGCCACCA 
AAGACGCCTC CACCAAAATG CATGGATTGC AGACAATATT TGGATGATCC AGACCTCAAG 
TACTTTCAGG GTGATCCAGA TGATGCTCTA GACGAGCCAG AGATGTTAAC AGATGAGCGA 
TTATCACTTT TTGAGTCCAA TGAGGATGGC TTTGAGAGCT ATGATGACCT CCCACAACAT 
AAAGTCACAT GCTTCAGTGT TTATGATAAA AGGGGCCATT TATGTCCTTT TGATTCTGGT 
CTGATTGAGA AGAATGTCGA GCTGTACTTC AGTGCGGTTG TAAAACCCAT TTATGATGAC 
AGCCCTTCTC TGGATGGTGG TGTACGAGCA AAGAAACTTG GTCCCATTAA CGCTTGGTGG 
ATAACTGGTT TTGATGGAGG AGAAAAGGCT TTGATTGGGT TTACAACGGC TTTTGCAGAC 
TACATCCTGA TGGATCCCAG TGAAGAGTAC AGCAGCATCT TTGCCCTGAT CGAGGAGAAG 
ATCTACATGA GTAAGATAGT TGTGGAATTC CTTCAAAATA ACCCTGATGT GAGCTATGAA 
GATCTGCTCA ACAAGATTGA GACTGCCGTC CCACCTTCAG CCTTAAACTT TAACCGCTTC 
ACAGAAGACT CGTTGTTAAG GCATGCACAG TTTGTGGTGG AACAAGTGGA GAGCTATGAT 
GAGGCTGGTG ACAGTGATGA GCAGCCTGTT ATTGTAACTC CCTGCATGAG GGACCTCATA 
AAGCTAGCTG GTGTTACTCT GGGCAAAAGA CGAGCAGCAA GAAGACAAAC AATCAGACAT 
CCTACAAAGA TAGAGAAGGA CAAAGGACCG ACAAAGGCCA CTACCACAAG ACTTGTGTAT 
CAGATTTTTG ACACATTCTT CTTCGAACAA ATCGAAAAGG ATGCAGAGAA GGAAAATGGC 
ATCAAGCGCA GGGCTTGTGG TGTTTGTGAG GTGTGTCAGC AACCAGACTG TGGCCAGTGC 
AAAGCTTGCC AGGCTATGTT GAAGTTTGGT GGGGCAGGCC GTACCAAACA AGCCTGCATG 
CAGAGAAGGT GTCCAAACCT GGCAGTGAAA GAAGCCGATG AAGATGAGGA AGTTGAAGAT 
GTTCTGCCAG AAATGCCATC ACCCAAAAAG ATCCTTCAGG GAAAGAAGAA AAAACTGGAG 
AAGAAAAACA GGATCTCTTG GGTGGGGGAT CCAGTTAAGA CTGAAGGGAA GAAAGAATAT 
TACCTGAAGG TGTCTATTGA CTCTGAAATA TTGGAAGTTG GAGATTGTGT GTCAGTGAGT 
CCTGATAATC CAACAGAGCC TTTGTACTTG GCAAGGATCA CATCCATGTG GGAGGAAGGC 
TGTGGACAAA TGTTCCATGC ACACTGGTTC TGTCTTGGCA CAGATACAGT CCTGGGCGCT 
ACATCTGACC CTCTCGAGCT CTTTCTTGTG GATGAATGCG AGGACATGCA GCTGTCTTAT 
ATTCATGGCA AAGTGAATGT ACTCTACAAA GCACCATCTG ACAACTGGTT CATGGAGGGA 
GGAACAGACA CTGAAATAAA AGTTGTGGAA GATGATGGAA ACACTTACTT CTACCAGTTG 
TGGTATGATC CAGAATATGC CCGGTTTGAG ACCCCACCCA AGCCTCAGTC TACAGAAGAC 
AATAAGTATA AGTTCTGCAC CAGTTGTGCC CGTCTGGATG AAATAAGGCA GAAAGAGATA 
CCCAGAGTAA GCAATCCCGT AGAGGAGCTC GACTCTAAAA TTTGCTACAG TACAGCTACA 
AAGAATGATG TACATTATAA GGTTGGAGAT GGAGTGCTAC ACTTCCCTGA TGCCTTTTCA 
TTTAGTGTAA AACTGGGTAG CCCTATGAAG CGACCCCAGA GGAAGGATGA TGTGGATGAA 
GATCTATACC CAGAATACTA CCGCAAATCA TCAGACTACA TTAAGGGTAG CAACCTGGAT 
GCTCCAGAAC CGTACCGATT GGGTCGCATT AAGGAGATCT TCTGCAATAA GAGAAGTAAT 
GGAAAGCCTA ATGAGGCAGA TATAAAGCTG CGGATTTACA AGTTTTACAG ACCTGAAAAT 
ACTCACAAAG GTATGAAAGC AAGTTACCAT TCAGACATAA ACATGGTTTA CTGGAGCGAT 
GAAGAGGCTG TAGTGGAGTT TAAGGCGGTG CAAGGACATT GTACAGTAGA ATATGGTGAA 
GATCTAACCG AATCCATTCA GGAGTATTCT GCTGGAGGAT CAGATCGCTT TTACTTCCTT 
GAGGCCTATA ATGCAAAGAC CAAAAGCTTT GAAGATCCTC CAAACCATGC TCGCGGTGCT 
GTTAACAAAG GCAAAGGAAA GGGCAAAGGA AAAGGCAAAG GCAAAACTCC TTCAAAGTCA 
GAGAATGAAC AGCTGAACTC TGGTGATAAA CTGCCTAAAC TGCGCACCTT GGATGTATTT 
TCTGGTTGTG GAGGTCTTTC TGAAGGTTTT CACCAAGCAG GCATATCTGA GACAAACTGG 
GCAATAGAGA TGTGGGAGCC TGCAGCCCAG GCCTTCAGAC TGAACAATCC TGGTACTACA 
GTGTTTACTG AAGACTGCAA CATTCTCCTG AAACTAGTCA TGTCTGGGGA AAAAACAAAT 
TCCCTAGGGC AGCGGTTGCC GCAGAAAGGC GACGTGGAAA TGCTCTGCGG AGGGCCCCCC 
TGCCAAGGTT TTAGTGGCAT GAACCGGTTT AACTCAAGAA CCTATTCAAA GTTCAAGAAC 
TCACTGGTGG TCTCGTATCT CAGCTACTGT GACTATTACA GACCTAAATA CTTCCTCTTG 
GAAAACGTCC GGAACTTTGT ATCATTCAAA AAATCGATGG TGCTTAAGCT AACACTCCGC 
TGCCTTGTAC GCATGGGCTA CCAGTGCACG TTTGGTGTTC TTCAGGCTGG CCAGTATGGT 
GTAGCACAGA CCCGCAGGAG AGCCATCGTA CTCGCTGCTG CTCCAGGAGA GAAGCTTCCA 
ATGTTCCCTG AGCCTTTGCA TGTGTTTGCT CCACGTGCTT GTACATTGAG TGTTGTAGTG 
GATGAAAAGA AATATGTCAG CAACACCACA AGAACGAACT CCAGCCTTTT CCGAACAATC 
ACTGTCCGGG ATACTATGTC TGACCTTCCA GAAATTCGCA ATGGAGCCTC TGCTCTGGAA 
ATCTCTTACA ACGGGGAGCC TCAGTCCTGG TTCCAGAGGC AGATTCGTGG CTCACAGTAC 
CAACCAATAC TCAGGGATCA TGTCTGTAAG GACATGAGCG CTTTAGTTGC TGCCAGAATG 
CGACACATAC CTCTTGCTCC AGGCTCAGAC TGGCGGGATC TCCCCAACAT GGAAGTTCGT 
TTGTCCGATG GCACAACCTC TAGAAAGTTG CGTTATACTC ATCATGACAA GAAAAATGGG 
CGCAGTGGCA CTGGTGCATT AAGAGGAGTC TGTTGTTGTT CTGAAGGCAA ACAATGTGAC 
CCTGCAGACC GGCAGTTTGG CACACTGATT CCCTGGTGCC TGCCGCACAC TGGAAACCGA 
CACAATCACT GGGCTGGCTT GTATGGAAGA CTGGAGTGGG ACAGCTTCTT CAGCACCACT 
GTTACTAACC CTGAGCCCAT GGGCAAACAG GGCCGTGTTC TTCACCCAGA GCAGCATCGT 
GTGGTGAGTG TCCGGGAATG TGCTCGGTCC CAAGGCTTTC CTGACACCTA CAGACTGTTT 
GGTAATATCT TGGATAAACA TAGGCAGGTG GGTAACGCTG TGCCGCCACC TCTGTCAAGA 
GCAATCGGCT TAGAGATAAA GTCGTGTGTT TTGACAAGGA TGAAAGAGAA TGGAACGGAA 
ACCGTGAAGG CTGAAAAGAT GGAAACAGAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001084021.1
CDS	260..4732
Translation	MPAQSTSLALPADVRKRLKDLKRDQDGMTEKEHVQQKLSLVLGFLEADARNKLNDLESKLSSEELSEEGYLTKVKSLLGKQLSFENVDLALNGETNGCSTNGTCGSDEEDVQLSESNTSGVKNRKPRKSKVNGENKKSPARARPSRSTAGKQPTILSMFSKGSTKRKSSDDEKDTDVPADADQPEEKEKEEKRIKIEVNESEDKRSDAEEGKKAKPVQPPKTPPPKCMDCRQYLDDPDLKYFQGDPDDALDEPEMLTDERLSLFESNEDGFESYDDLPQHKVTCFSVYDKRGHLCPFDSGLIEKNVELYFSAVVKPIYDDSPSLDGGVRAKKLGPINAWWITGFDGGEKALIGFTTAFADYILMDPSEEYSSIFALIEEKIYMSKIVVEFLQNNPDVSYEDLLNKIETAVPPSALNFNRFTEDSLLRHAQFVVEQVESYDEAGDSDEQPVIVTPCMRDLIKLAGVTLGKRRAARRQTIRHPTKIEKDKGPTKATTTRLVYQIFDTFFFEQIEKDAEKENGIKRRACGVCEVCQQPDCGQCKACQAMLKFGGAGRTKQACMQRRCPNLAVKEADEDEEVEDVLPEMPSPKKILQGKKKKLEKKNRISWVGDPVKTEGKKEYYLKVSIDSEILEVGDCVSVSPDNPTEPLYLARITSMWEEGCGQMFHAHWFCLGTDTVLGATSDPLELFLVDECEDMQLSYIHGKVNVLYKAPSDNWFMEGGTDTEIKVVEDDGNTYFYQLWYDPEYARFETPPKPQSTEDNKYKFCTSCARLDEIRQKEIPRVSNPVEELDSKICYSTATKNDVHYKVGDGVLHFPDAFSFSVKLGSPMKRPQRKDDVDEDLYPEYYRKSSDYIKGSNLDAPEPYRLGRIKEIFCNKRSNGKPNEADIKLRIYKFYRPENTHKGMKASYHSDINMVYWSDEEAVVEFKAVQGHCTVEYGEDLTESIQEYSAGGSDRFYFLEAYNAKTKSFEDPPNHARGAVNKGKGKGKGKGKGKTPSKSENEQLNSGDKLPKLRTLDVFSGCGGLSEGFHQAGISETNWAIEMWEPAAQAFRLNNPGTTVFTEDCNILLKLVMSGEKTNSLGQRLPQKGDVEMLCGGPPCQGFSGMNRFNSRTYSKFKNSLVVSYLSYCDYYRPKYFLLENVRNFVSFKKSMVLKLTLRCLVRMGYQCTFGVLQAGQYGVAQTRRRAIVLAAAPGEKLPMFPEPLHVFAPRACTLSVVVDEKKYVSNTTRTNSSLFRTITVRDTMSDLPEIRNGASALEISYNGEPQSWFQRQIRGSQYQPILRDHVCKDMSALVAARMRHIPLAPGSDWRDLPNMEVRLSDGTTSRKLRYTHHDKKNGRSGTGALRGVCCCSEGKQCDPADRQFGTLIPWCLPHTGNRHNHWAGLYGRLEWDSFFSTTVTNPEPMGKQGRVLHPEQHRVVSVRECARSQGFPDTYRLFGNILDKHRQVGNAVPPPLSRAIGLEIKSCVLTRMKENGTETVKAEKMETD

Target ORF information:

RefSeq Version	NM_001090552.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis DNA methyltransferase 1 L homeolog (dnmt1.L), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001090552.1

ORF Insert Sequence:

ATGCCGGCCC AGTCCACTTC CTTGGCTCTG CCTGCTGATG TCAGGAAACG GCTAAAGGAT 
TTGAAGAGGG ATCAGGATGG AATGACTGAG AAGGAACATG TACAGCAGAA ACTGAGTTTG 
GTGCTTGGCT TCCTTGAAGC AGATGCTAGA AACAAACTGA ATGATCTCGA GAGCAAGTTG 
AGCAGTGAAG AACTGTCTGA GGAAGGATAC CTTACAAAAG TGAAGTCTCT TCTGGGGAAG 
CAGTTGAGCT TTGAAAACGT AGACCTTGCT TTGAACGGAG AAACAAATGG CTGCTCCACC 
AATGGTACCT GCGGCAGTGA TGAGGAAGAT GTACAGTTGT CGGAGTCTAA TACTTCTGGT 
GTTAAAAACC GCAAACCTAG GAAAAGTAAA GTTAATGGAG AAAACAAGAA ATCACCAGCC 
CGTGCCCGCC CATCAAGAAG CACTGCAGGC AAACAACCCA CCATCCTCTC CATGTTCTCT 
AAAGGGTCAA CCAAGCGGAA ATCCAGTGAT GATGAGAAAG ATACAGATGT TCCAGCTGAT 
GCAGACCAAC CAGAAGAGAA GGAAAAAGAA GAGAAAAGAA TAAAAATCGA AGTTAATGAA 
AGCGAGGACA AACGGTCTGA TGCGGAAGAA GGTAAAAAGG CAAAACCTGT TCAGCCACCA 
AAGACGCCTC CACCAAAATG CATGGATTGC AGACAATATT TGGATGATCC AGACCTCAAG 
TACTTTCAGG GTGATCCAGA TGATGCTCTA GACGAGCCAG AGATGTTAAC AGATGAGCGA 
TTATCACTTT TTGAGTCCAA TGAGGATGGC TTTGAGAGCT ATGATGACCT CCCACAACAT 
AAAGTCACAT GCTTCAGTGT TTATGATAAA AGGGGCCATT TATGTCCTTT TGATTCTGGT 
CTGATTGAGA AGAATGTCGA GCTGTACTTC AGTGCGGTTG TAAAACCCAT TTATGATGAC 
AGCCCTTCTC TGGATGGTGG TGTACGAGCA AAGAAACTTG GTCCCATTAA CGCTTGGTGG 
ATAACTGGTT TTGATGGAGG AGAAAAGGCT TTGATTGGGT TTACAACGGC TTTTGCAGAC 
TACATCCTGA TGGATCCCAG TGAAGAGTAC AGCAGCATCT TTGCCCTGAT CGAGGAGAAG 
ATCTACATGA GTAAGATAGT TGTGGAATTC CTTCAAAATA ACCCTGATGT GAGCTATGAA 
GATCTGCTCA ACAAGATTGA GACTGCCGTC CCACCTTCAG CCTTAAACTT TAACCGCTTC 
ACAGAAGACT CGTTGTTAAG GCATGCACAG TTTGTGGTGG AACAAGTGGA GAGCTATGAT 
GAGGCTGGTG ACAGTGATGA GCAGCCTGTT ATTGTAACTC CCTGCATGAG GGACCTCATA 
AAGCTAGCTG GTGTTACTCT GGGCAAAAGA CGAGCAGCAA GAAGACAAAC AATCAGACAT 
CCTACAAAGA TAGAGAAGGA CAAAGGACCG ACAAAGGCCA CTACCACAAG ACTTGTGTAT 
CAGATTTTTG ACACATTCTT CTTCGAACAA ATCGAAAAGG ATGCAGAGAA GGAAAATGGC 
ATCAAGCGCA GGGCTTGTGG TGTTTGTGAG GTGTGTCAGC AACCAGACTG TGGCCAGTGC 
AAAGCTTGCC AGGCTATGTT GAAGTTTGGT GGGGCAGGCC GTACCAAACA AGCCTGCATG 
CAGAGAAGGT GTCCAAACCT GGCAGTGAAA GAAGCCGATG AAGATGAGGA AGTTGAAGAT 
GTTCTGCCAG AAATGCCATC ACCCAAAAAG ATCCTTCAGG GAAAGAAGAA AAAACTGGAG 
AAGAAAAACA GGATCTCTTG GGTGGGGGAT CCAGTTAAGA CTGAAGGGAA GAAAGAATAT 
TACCTGAAGG TGTCTATTGA CTCTGAAATA TTGGAAGTTG GAGATTGTGT GTCAGTGAGT 
CCTGATAATC CAACAGAGCC TTTGTACTTG GCAAGGATCA CATCCATGTG GGAGGAAGGC 
TGTGGACAAA TGTTCCATGC ACACTGGTTC TGTCTTGGCA CAGATACAGT CCTGGGCGCT 
ACATCTGACC CTCTCGAGCT CTTTCTTGTG GATGAATGCG AGGACATGCA GCTGTCTTAT 
ATTCATGGCA AAGTGAATGT ACTCTACAAA GCACCATCTG ACAACTGGTT CATGGAGGGA 
GGAACAGACA CTGAAATAAA AGTTGTGGAA GATGATGGAA ACACTTACTT CTACCAGTTG 
TGGTATGATC CAGAATATGC CCGGTTTGAG ACCCCACCCA AGCCTCAGTC TACAGAAGAC 
AATAAGTATA AGTTCTGCAC CAGTTGTGCC CGTCTGGATG AAATAAGGCA GAAAGAGATA 
CCCAGAGTAA GCAATCCCGT AGAGGAGCTC GACTCTAAAA TTTGCTACAG TACAGCTACA 
AAGAATGATG TACATTATAA GGTTGGAGAT GGAGTGCTAC ACTTCCCTGA TGCCTTTTCA 
TTTAGTGTAA AACTGGGTAG CCCTATGAAG CGACCCCAGA GGAAGGATGA TGTGGATGAA 
GATCTATACC CAGAATACTA CCGCAAATCA TCAGACTACA TTAAGGGTAG CAACCTGGAT 
GCTCCAGAAC CGTACCGATT GGGTCGCATT AAGGAGATCT TCTGCAATAA GAGAAGTAAT 
GGAAAGCCTA ATGAGGCAGA TATAAAGCTG CGGATTTACA AGTTTTACAG ACCTGAAAAT 
ACTCACAAAG GTATGAAAGC AAGTTACCAT TCAGACATAA ACATGGTTTA CTGGAGCGAT 
GAAGAGGCTG TAGTGGAGTT TAAGGCGGTG CAAGGACATT GTACAGTAGA ATATGGTGAA 
GATCTAACCG AATCCATTCA GGAGTATTCT GCTGGAGGAT CAGATCGCTT TTACTTCCTT 
GAGGCCTATA ATGCAAAGAC CAAAAGCTTT GAAGATCCTC CAAACCATGC TCGCGGTGCT 
GTTAACAAAG GCAAAGGAAA GGGCAAAGGA AAAGGCAAAG GCAAAACTCC TTCAAAGTCA 
GAGAATGAAC AGCTGAACTC TGGTGATAAA CTGCCTAAAC TGCGCACCTT GGATGTATTT 
TCTGGTTGTG GAGGTCTTTC TGAAGGTTTT CACCAAGCAG GCATATCTGA GACAAACTGG 
GCAATAGAGA TGTGGGAGCC TGCAGCCCAG GCCTTCAGAC TGAACAATCC TGGTACTACA 
GTGTTTACTG AAGACTGCAA CATTCTCCTG AAACTAGTCA TGTCTGGGGA AAAAACAAAT 
TCCCTAGGGC AGCGGTTGCC GCAGAAAGGC GACGTGGAAA TGCTCTGCGG AGGGCCCCCC 
TGCCAAGGTT TTAGTGGCAT GAACCGGTTT AACTCAAGAA CCTATTCAAA GTTCAAGAAC 
TCACTGGTGG TCTCGTATCT CAGCTACTGT GACTATTACA GACCTAAATA CTTCCTCTTG 
GAAAACGTCC GGAACTTTGT ATCATTCAAA AAATCGATGG TGCTTAAGCT AACACTCCGC 
TGCCTTGTAC GCATGGGCTA CCAGTGCACG TTTGGTGTTC TTCAGGCTGG CCAGTATGGT 
GTAGCACAGA CCCGCAGGAG AGCCATCGTA CTCGCTGCTG CTCCAGGAGA GAAGCTTCCA 
ATGTTCCCTG AGCCTTTGCA TGTGTTTGCT CCACGTGCTT GTACATTGAG TGTTGTAGTG 
GATGAAAAGA AATATGTCAG CAACACCACA AGAACGAACT CCAGCCTTTT CCGAACAATC 
ACTGTCCGGG ATACTATGTC TGACCTTCCA GAAATTCGCA ATGGAGCCTC TGCTCTGGAA 
ATCTCTTACA ACGGGGAGCC TCAGTCCTGG TTCCAGAGGC AGATTCGTGG CTCACAGTAC 
CAACCAATAC TCAGGGATCA TGTCTGTAAG GACATGAGCG CTTTAGTTGC TGCCAGAATG 
CGACACATAC CTCTTGCTCC AGGCTCAGAC TGGCGGGATC TCCCCAACAT GGAAGTTCGT 
TTGTCCGATG GCACAACCTC TAGAAAGTTG CGTTATACTC ATCATGACAA GAAAAATGGG 
CGCAGTGGCA CTGGTGCATT AAGAGGAGTC TGTTGTTGTT CTGAAGGCAA ACAATGTGAC 
CCTGCAGACC GGCAGTTTGG CACACTGATT CCCTGGTGCC TGCCGCACAC TGGAAACCGA 
CACAATCACT GGGCTGGCTT GTATGGAAGA CTGGAGTGGG ACAGCTTCTT CAGCACCACT 
GTTACTAACC CTGAGCCCAT GGGCAAACAG GGCCGTGTTC TTCACCCAGA GCAGCATCGT 
GTGGTGAGTG TCCGGGAATG TGCTCGGTCC CAAGGCTTTC CTGACACCTA CAGACTGTTT 
GGTAATATCT TGGATAAACA TAGGCAGGTG GGTAACGCTG TGCCGCCACC TCTGTCAAGA 
GCAATCGGCT TAGAGATAAA GTCGTGTGTT TTGACAAGGA TGAAAGAGAA TGGAACGGAA 
ACCGTGAAGG CTGAAAAGAT GGAAACAGAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Lsh Is Essential for Maintaining Global DNA Methylation Levels in Amphibia and Fish and Interacts Directly with Dnmt1.
BioMed research international2015740637(2015)
Dunican DS,Pennings S,Meehan RR

MBD4 and MLH1 are required for apoptotic induction in xDNMT1-depleted embryos.
Development (Cambridge, England)136(13)2277-86(2009 Jul)
Ruzov A,Shorning B,Mortusewicz O,Dunican DS,Leonhardt H,Meehan RR

xDnmt1 regulates transcriptional silencing in pre-MBT Xenopus embryos independently of its catalytic function.
Development (Cambridge, England)135(7)1295-302(2008 Apr)
Dunican DS,Ruzov A,Hackett JA,Meehan RR

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

Isolation and expression of a Xenopus laevis DNA methyltransferase cDNA.
Journal of biochemistry120(6)1182-9(1996 Dec)
Kimura H,Ishihara G,Tajima S

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dnmt1.L cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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