LOC108700359 cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC108700359
Entrez Gene ID	108700359
Full Name	Rho guanine nucleotide exchange factor 11 S homeolog
General protein information	Preferred Names Rho guanine nucleotide exchange factor 11 S homeolog Names rho guanine nucleotide exchange factor 11-like
Gene Type	protein-coding
Organism	Xenopus laevis(African clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018233487.1	XP_018088976.1	rho guanine nucleotide exchange factor 11-like isoform X6
XM_018233483.1	XP_018088972.1	rho guanine nucleotide exchange factor 11-like isoform X2
XM_018233486.1	XP_018088975.1	rho guanine nucleotide exchange factor 11-like isoform X5
XM_018233482.1	XP_018088971.1	rho guanine nucleotide exchange factor 11-like isoform X1
XM_018233485.1	XP_018088974.1	rho guanine nucleotide exchange factor 11-like isoform X4
XM_018233484.1	XP_018088973.1	rho guanine nucleotide exchange factor 11-like isoform X3

Pathways from BioSystems
Gene Ontology

The following LOC108700359 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC108700359 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAf44534	XM_018233482.1 Latest version!	Xenopus laevis rho guanine nucleotide exchange factor 11-like (LOC108700359), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OAf44535	XM_018233483.1 Latest version!	Xenopus laevis rho guanine nucleotide exchange factor 11-like (LOC108700359), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OAf44536	XM_018233484.1 Latest version!	Xenopus laevis rho guanine nucleotide exchange factor 11-like (LOC108700359), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OAf44537	XM_018233485.1 Latest version!	Xenopus laevis rho guanine nucleotide exchange factor 11-like (LOC108700359), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OAf44538	XM_018233486.1 Latest version!	Xenopus laevis rho guanine nucleotide exchange factor 11-like (LOC108700359), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OAf44539	XM_018233487.1 Latest version!	Xenopus laevis rho guanine nucleotide exchange factor 11-like (LOC108700359), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC108700359 ( XM_018233482.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018088971.1 Xenopus laevis rho guanine nucleotide exchange factor 11-like (LOC108700359), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAf44534
Clone ID Related Accession (Same CDS sequence)	XM_018233482.1
Accession Version	XM_018233482.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1474214400000
Organism	Xenopus laevis(African clawed frog)
Product	rho guanine nucleotide exchange factor 11-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030739.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATCA GTTCCGCACA GACTTCAATA GACCGATCCG CTTCTAAACG CTATCCTCGT 
TTGCCAAGGT TGAGTAGCCT GTCATCGGCT AGTGACGGAT CTGCCGAGCG AAGGTCTCCT 
GGACACCAGC GTCAGCCTTG TAACGAGAGT AACGAGTCTA CAGGCCTGGT CCAGCGCTGT 
GTCATTGTTC AAAGGGACCA AAATGGATTT GGTTTCACGG TCAGCGGAGA TCGAATTGTT 
CTGGTTCAGT CTGTACGTCC AGGTGGGGCA GCAATGCGAG CTGGGGTGCA GGAGGGAGAC 
CGGATTGTGA AGGTCAACGG CACGCTGGTG GCTAACAGCT CACACATCGA AGTGGTCAAG 
CTGATTAAAT CCGGTGCTTA CGTGGCTCTC ACTCTCCTGG GTTCTCCACC TCCATCCATG 
GGCATGTCTG GGTATCAGCA AGACTTGAGC TCCTCTGTGT CCCCACGTAA CATATTAGTG 
TCGCCAATTC CTCCACCTCC ACCACCTTTG CCGCAGTCGC ATCACATCAC AGGACCTAAA 
CCCTTACAGG ACCTCGATTT CCAAAAGCAT GCTACGGAAA TTCTTTGGAA CATGTTAAAG 
CAGGAGGAAG CAGATCTACA GAATCTGTGT GATGTGTATA ACAGAAGCCC TAACCCCAGC 
CTGCAGGAGC AGATAGAGAG GGCCAGGAAG AGGGTGCATC AGCTGCAGCA TAAGATAAGG 
CCAGAAATAC CCAGTACTGG GAATTTGCTG CGTTCATACA GTGACTGCAG CTCTGTGGTG 
TATAAAGTCA AAGAAGGTCG CTTTTCTGTT GATTCTCAGG ATGGAGACAG TGGCCTGGAT 
TCGGGAACTG AACGGATATC ATCGTTGAGT GAGTATTCCA TGAATCGGAA TTCTGTCCTG 
TCAGACCTCG GAATGGACAG CTCCCACACC TCCCCAATCA TCCCAACGAA ACTGTTTCAC 
CACCACCATC GGCGGCACGG GTCAGACACC ACCTTTATTG CCACGTCTGA GCAGGTATCA 
GATCATAGGA GGTCTACGAT AATCGGGCCA GAAGAGGACT GTGACCATGG ATATTACATG 
ACAGAGCAGT CAGATGGTCT CTTCCAAGAT CTGGAAAAGT TAAAGACCCG GCCAGCCCAT 
ATGGGGGTGT TTCTACGTTA TATCTTCTCC CAGGCTGATC CAAACCCACT GCTCTTTTAC 
CTGAGTGCTG AGGCCTGTCA GCATTTGAGT ACAACAGACG CCTTCACATT GAGGAAAGAA 
GTCTGGAACA TCTTCCTTGA CAAAAGTGCA CCACTGAGGG TTAAAATCCC TGATCAGCTA 
CTGAATGAAA TTGAATCCCG TTTGCGGAAC TCGGAAGATA TCCGTGGTGT GTTTAGTGAA 
GCCCAGGAGG CGATGCTGCC AGAGATACAG GAGCAGATAC AGGATTACAG GACGAAAAGG 
ACCATGGGAT TAGGGAGCCT GTACGGGGAG AATGACCTGT TCGACCTTGA GATTGACCCT 
CACAGAGAAC GTCATGTTGC TGCTAAACAG ATTGCACAGC TGGAGGACAT ACTATCAAAG 
TATGAGAAAG AGATCAGCTC GCCCATGGCC TTTGCCTTGA GCATGTATGT GAAGTACGCT 
GGGATCCACA CTCAAGATAT CCGTCCCGCC AGCATCTCCG AAAAAAGCAT CCCCGACAGG 
GAAAAGTGGC TCCCGTTCTT CCCTAAGAAC AAGAAAAGCA GCAACGCCAA AAAGGATAAA 
GAGTCGGCTG AGGACAAGAA GCGCAACCCC ATCCTTAAGT ATATTAACAA GCCCAAGAGT 
ACGTCTCAGA GTACATTTCA TGTTCCTTTG TCCCCTGGTG AAGTTAAACC CGGGAATGTG 
AGAAACATTA TCCAGCACTT TGAAAACAAC CAACAATATG ATGTTCCCGA ACTTAACCTA 
CAGCGGCTTT CAACCGGGAG CTTCCCTGAA GATCTACTGG GCTCTGACAG CATCAGGTCG 
GAGGTGAAAC TGGGCCGGTC AGAAAGCTTA AAGGGACGGG AAGAGTTGAA GAAATCCCGC 
AAATTGGACA ATGTTCCTCG ATCTCGCAGT GACGTAGATA TGGACGCGGC AGCTGAGGCT 
ACACGGCTAC ACCAGTCGGC TTCATCCTCT GCATCCAGCC TATCCGCAAG GTCATTGGAT 
AATCCTACCC CACCCTATAC TCCCAAATTA TGTCGAAGGA GCATGGAGTC GCCCAATTTG 
GGGTTGACTG GAGAATTGCT GCTCTCCAAC CTGCAGGAGG ACGACCTGGG TCACCTCTCT 
GACCTTGACC CTGAGCCCGA CGCACAGAAC TGGCAGCATA CGGTGGGTCG GGACGTGGCG 
TTGCACCTGC CACCCAAAGA GAGGGCACGG CAAGAGGTGA TACATGAGCT GTTTGTGACA 
GAAGCCTCCC ACCTTCGGGT CCTACGAGTT CTGGATCTCG TTTTCTACCA GAAGATGCGC 
AAGGAGAACC TGCTGACCCC AGATGAGCTC TGGCTTGTCT TCCCCAATCT CCCAGAACTG 
ATTGAGATCC ACAACTCGCT GGCAGAGTCA ATGAAGCGGG TGAGAGAAGA AGGGCCAATC 
ATCAAAGAGA TCGGTGACCT CATGCTTTTG CAGTTTGATG GAGCTTCAGG GGAGGAAGTC 
CGAATGGAAG CAGCCAAGTT CTGCTCGTAC CAGACATCTG CCCTTGAGTT GATCAAAGCC 
AAAAAGAGGA AAGAGACCCG GTTCAGTCTC TTCATGCAGG AAGCTGAAAG CAACCCCCAG 
TGCCGACGAC TGCAGCTGAA GGACCTGATT GTGTCTGAGA TGCAACGGCT GACCAAGTAC 
CCTCTGCTGC TGGAAAACGT CCTTAAGCAC ACAGACTCCG GGACTCAGGA ACATGAAAAG 
CTGTACCGTT GCCGTGATCG GTGTCGAGAT ATTCTGAAAT ATGTCAACGA GTCCGTCAAA 
CAGGCAGAAA ATGAACATCG ACTGGCTGAG TATCAGAAGC GACTGGACGC AAGCCACTTG 
GAGCGGTCGA ATAATCCTCT AGCTGGAGAG TTTAAGAACT TAGACCTGAC CACAAGGCGA 
ATGATACATG AGGGGCCTCT CACCTGGAGG GTAACAAAAG ACAAGAATAT AGACTTGCAT 
GTTCTCTTGC TGGAAGATCT CCTCGTATTA CTTCAGAAGC AGGATGAGAA GCTGGTGCTC 
AAATGCCATA GCAAGACCGC CATGGTCTCC ACCGACACCA AACAAACCTT TAGTCCGGTC 
ATTAAGCTGA ATTCTGTCCT TGTTCGCTCG GTGGCTACAG ATAAAAGAGC CCTGTTTATT 
ATCTGCACTT CAGACTTGGG ACCTCAGATC TACGAGCTGG TGGCACTGAC ATCCTCAGAG 
AAAAACACGT GGAAGGATTT ATTGGAAGAA GCAACCAATG GTGCCATGAG GAATCCTCCC 
GGTGTCACCA AACGGAAATC CCAGGACATC AATAGACCAA CCTCTGCCAG CATGGTCCTA 
CAGAGAGCCC ACATGCCCAA AGTCTTGGCA GAAGGAGTTC TAAGACATAA CAGTGAGGAC 
CCTCAATCAG GTGATGAGAG CGCTGGGGAA TTCTTAGGCC ACCAAAAGCC AGATGTCGTC 
CTAGAGGAAT CGGAAAGGGA GGAAGATGAA GAAGATGAAG CTCGGACACC TACCCTGCTG 
CCTATCCCAT CTAAAGAGGA GGATGCCGGA GGTGGGACAC GGGGGTTAGA CTCTTCCAAC 
CATATTCCCT ACCAGTGGCC TGCAGTGACT GAAGGGCTGG CTGAGTCTGC ACTGGATGAT 
GTTCATAACC TCCGCCAGTT GATCTTGAAG AACATGCTCC ACCACGGCCA TTCCGAGTAC 
AACTCTTGCT CTGCCCCAGT GGCTGAGCCT GAAGATGACC TCACGCCTAC GCCTTCCATC 
ATTGGAGGAC ACGGGCACCC ATGGGACACT ATGACAGACG CAGTATTAGA AAATCTAGGC 
GGAGATCAGA CTACTGAAGA GAAGGAGACC TCTGTTCCAC CATCAGTTAG CAGCTCTGAG 
AGCAGCAGGT CTGTTTTGAA CTTTCCTCAT GGAGATAGAC CAGGTTCCAG AGATGGGGAG 
GAGACTGCTT GGAGTTTGGT TGAACGCTAC CCACAAAGCA GTTCTGTTCA TCAGAACCAA 
TCAGAGCAAC TACACTGTGA GGACCATAGT AACCAAAGCT ACAGAGTTGT TAGAACAGCT 
GAGGTGGTGG GAAATTATGA TGTCATACCG TTGCCAGACA GCAGCCAATC ACAGAGTGTG 
TTGCAGGAAG CTGGCGGTGG AGCTATTGTA GATGGAAATT TCTTTTATGT AACTGCGTCT 
ACGGCTTCTG CAGCTTCTGC AAAGGAAGAT AATGAAGTCT CAGAATCCCA AATGAACTCT 
GACCGGGCGG GCTTGTCCTT CTTGGAATCT CAAGAGTCAA ACCACACGCC AACTTACCAG 
CCAGAACTTG CCACCCCACC ATCAAGGAAT ACAGAAAGAG ATCCACTTTC ATCGGATCTC 
CTGCCCCCAA AACTTGTCAT TCAGGAAGTA GATATTATCT TCAGGACGAT AGAGCAGCTG 
ACGGCCAAAC TCCACAGGCT GAAGGTAGTA GAGTCAGCCC ATCAGGAGCT TCTGCGATCC 
TTAGGAAGAA GTGCCACAGA AGACCATTAC CATGAGAGAG AGCACATGGC AGAGAAGGAT 
AATCAAGCTC AGAAGCATCA GGGGTCCAAT TTGAACAGCT CCTCTCTATT CTTTGTGAAG 
AACAGCATAT CGGTCTCGCT TCATCGAGAT GGCAATACAG ACCCACAGGA AGATGCTATA 
AATCTGGCCA TGGGAATATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018088971.1
CDS	1131..5951
Translation	MSISSAQTSIDRSASKRYPRLPRLSSLSSASDGSAERRSPGHQRQPCNESNESTGLVQRCVIVQRDQNGFGFTVSGDRIVLVQSVRPGGAAMRAGVQEGDRIVKVNGTLVANSSHIEVVKLIKSGAYVALTLLGSPPPSMGMSGYQQDLSSSVSPRNILVSPIPPPPPPLPQSHHITGPKPLQDLDFQKHATEILWNMLKQEEADLQNLCDVYNRSPNPSLQEQIERARKRVHQLQHKIRPEIPSTGNLLRSYSDCSSVVYKVKEGRFSVDSQDGDSGLDSGTERISSLSEYSMNRNSVLSDLGMDSSHTSPIIPTKLFHHHHRRHGSDTTFIATSEQVSDHRRSTIIGPEEDCDHGYYMTEQSDGLFQDLEKLKTRPAHMGVFLRYIFSQADPNPLLFYLSAEACQHLSTTDAFTLRKEVWNIFLDKSAPLRVKIPDQLLNEIESRLRNSEDIRGVFSEAQEAMLPEIQEQIQDYRTKRTMGLGSLYGENDLFDLEIDPHRERHVAAKQIAQLEDILSKYEKEISSPMAFALSMYVKYAGIHTQDIRPASISEKSIPDREKWLPFFPKNKKSSNAKKDKESAEDKKRNPILKYINKPKSTSQSTFHVPLSPGEVKPGNVRNIIQHFENNQQYDVPELNLQRLSTGSFPEDLLGSDSIRSEVKLGRSESLKGREELKKSRKLDNVPRSRSDVDMDAAAEATRLHQSASSSASSLSARSLDNPTPPYTPKLCRRSMESPNLGLTGELLLSNLQEDDLGHLSDLDPEPDAQNWQHTVGRDVALHLPPKERARQEVIHELFVTEASHLRVLRVLDLVFYQKMRKENLLTPDELWLVFPNLPELIEIHNSLAESMKRVREEGPIIKEIGDLMLLQFDGASGEEVRMEAAKFCSYQTSALELIKAKKRKETRFSLFMQEAESNPQCRRLQLKDLIVSEMQRLTKYPLLLENVLKHTDSGTQEHEKLYRCRDRCRDILKYVNESVKQAENEHRLAEYQKRLDASHLERSNNPLAGEFKNLDLTTRRMIHEGPLTWRVTKDKNIDLHVLLLEDLLVLLQKQDEKLVLKCHSKTAMVSTDTKQTFSPVIKLNSVLVRSVATDKRALFIICTSDLGPQIYELVALTSSEKNTWKDLLEEATNGAMRNPPGVTKRKSQDINRPTSASMVLQRAHMPKVLAEGVLRHNSEDPQSGDESAGEFLGHQKPDVVLEESEREEDEEDEARTPTLLPIPSKEEDAGGGTRGLDSSNHIPYQWPAVTEGLAESALDDVHNLRQLILKNMLHHGHSEYNSCSAPVAEPEDDLTPTPSIIGGHGHPWDTMTDAVLENLGGDQTTEEKETSVPPSVSSSESSRSVLNFPHGDRPGSRDGEETAWSLVERYPQSSSVHQNQSEQLHCEDHSNQSYRVVRTAEVVGNYDVIPLPDSSQSQSVLQEAGGGAIVDGNFFYVTASTASAASAKEDNEVSESQMNSDRAGLSFLESQESNHTPTYQPELATPPSRNTERDPLSSDLLPPKLVIQEVDIIFRTIEQLTAKLHRLKVVESAHQELLRSLGRSATEDHYHEREHMAEKDNQAQKHQGSNLNSSSLFFVKNSISVSLHRDGNTDPQEDAINLAMGI

Target ORF information:

RefSeq Version	XM_018233482.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis rho guanine nucleotide exchange factor 11-like (LOC108700359), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018233482.1

ORF Insert Sequence:

ATGAGCATCA GTTCCGCACA GACTTCAATA GACCGATCCG CTTCTAAACG CTATCCTCGT 
TTGCCAAGGT TGAGTAGCCT GTCATCGGCT AGTGACGGAT CTGCCGAGCG AAGGTCTCCT 
GGACACCAGC GTCAGCCTTG TAACGAGAGT AACGAGTCTA CAGGCCTGGT CCAGCGCTGT 
GTCATTGTTC AAAGGGACCA AAATGGATTT GGTTTCACGG TCAGCGGAGA TCGAATTGTT 
CTGGTTCAGT CTGTACGTCC AGGTGGGGCA GCAATGCGAG CTGGGGTGCA GGAGGGAGAC 
CGGATTGTGA AGGTCAACGG CACGCTGGTG GCTAACAGCT CACACATCGA AGTGGTCAAG 
CTGATTAAAT CCGGTGCTTA CGTGGCTCTC ACTCTCCTGG GTTCTCCACC TCCATCCATG 
GGCATGTCTG GGTATCAGCA AGACTTGAGC TCCTCTGTGT CCCCACGTAA CATATTAGTG 
TCGCCAATTC CTCCACCTCC ACCACCTTTG CCGCAGTCGC ATCACATCAC AGGACCTAAA 
CCCTTACAGG ACCTCGATTT CCAAAAGCAT GCTACGGAAA TTCTTTGGAA CATGTTAAAG 
CAGGAGGAAG CAGATCTACA GAATCTGTGT GATGTGTATA ACAGAAGCCC TAACCCCAGC 
CTGCAGGAGC AGATAGAGAG GGCCAGGAAG AGGGTGCATC AGCTGCAGCA TAAGATAAGG 
CCAGAAATAC CCAGTACTGG GAATTTGCTG CGTTCATACA GTGACTGCAG CTCTGTGGTG 
TATAAAGTCA AAGAAGGTCG CTTTTCTGTT GATTCTCAGG ATGGAGACAG TGGCCTGGAT 
TCGGGAACTG AACGGATATC ATCGTTGAGT GAGTATTCCA TGAATCGGAA TTCTGTCCTG 
TCAGACCTCG GAATGGACAG CTCCCACACC TCCCCAATCA TCCCAACGAA ACTGTTTCAC 
CACCACCATC GGCGGCACGG GTCAGACACC ACCTTTATTG CCACGTCTGA GCAGGTATCA 
GATCATAGGA GGTCTACGAT AATCGGGCCA GAAGAGGACT GTGACCATGG ATATTACATG 
ACAGAGCAGT CAGATGGTCT CTTCCAAGAT CTGGAAAAGT TAAAGACCCG GCCAGCCCAT 
ATGGGGGTGT TTCTACGTTA TATCTTCTCC CAGGCTGATC CAAACCCACT GCTCTTTTAC 
CTGAGTGCTG AGGCCTGTCA GCATTTGAGT ACAACAGACG CCTTCACATT GAGGAAAGAA 
GTCTGGAACA TCTTCCTTGA CAAAAGTGCA CCACTGAGGG TTAAAATCCC TGATCAGCTA 
CTGAATGAAA TTGAATCCCG TTTGCGGAAC TCGGAAGATA TCCGTGGTGT GTTTAGTGAA 
GCCCAGGAGG CGATGCTGCC AGAGATACAG GAGCAGATAC AGGATTACAG GACGAAAAGG 
ACCATGGGAT TAGGGAGCCT GTACGGGGAG AATGACCTGT TCGACCTTGA GATTGACCCT 
CACAGAGAAC GTCATGTTGC TGCTAAACAG ATTGCACAGC TGGAGGACAT ACTATCAAAG 
TATGAGAAAG AGATCAGCTC GCCCATGGCC TTTGCCTTGA GCATGTATGT GAAGTACGCT 
GGGATCCACA CTCAAGATAT CCGTCCCGCC AGCATCTCCG AAAAAAGCAT CCCCGACAGG 
GAAAAGTGGC TCCCGTTCTT CCCTAAGAAC AAGAAAAGCA GCAACGCCAA AAAGGATAAA 
GAGTCGGCTG AGGACAAGAA GCGCAACCCC ATCCTTAAGT ATATTAACAA GCCCAAGAGT 
ACGTCTCAGA GTACATTTCA TGTTCCTTTG TCCCCTGGTG AAGTTAAACC CGGGAATGTG 
AGAAACATTA TCCAGCACTT TGAAAACAAC CAACAATATG ATGTTCCCGA ACTTAACCTA 
CAGCGGCTTT CAACCGGGAG CTTCCCTGAA GATCTACTGG GCTCTGACAG CATCAGGTCG 
GAGGTGAAAC TGGGCCGGTC AGAAAGCTTA AAGGGACGGG AAGAGTTGAA GAAATCCCGC 
AAATTGGACA ATGTTCCTCG ATCTCGCAGT GACGTAGATA TGGACGCGGC AGCTGAGGCT 
ACACGGCTAC ACCAGTCGGC TTCATCCTCT GCATCCAGCC TATCCGCAAG GTCATTGGAT 
AATCCTACCC CACCCTATAC TCCCAAATTA TGTCGAAGGA GCATGGAGTC GCCCAATTTG 
GGGTTGACTG GAGAATTGCT GCTCTCCAAC CTGCAGGAGG ACGACCTGGG TCACCTCTCT 
GACCTTGACC CTGAGCCCGA CGCACAGAAC TGGCAGCATA CGGTGGGTCG GGACGTGGCG 
TTGCACCTGC CACCCAAAGA GAGGGCACGG CAAGAGGTGA TACATGAGCT GTTTGTGACA 
GAAGCCTCCC ACCTTCGGGT CCTACGAGTT CTGGATCTCG TTTTCTACCA GAAGATGCGC 
AAGGAGAACC TGCTGACCCC AGATGAGCTC TGGCTTGTCT TCCCCAATCT CCCAGAACTG 
ATTGAGATCC ACAACTCGCT GGCAGAGTCA ATGAAGCGGG TGAGAGAAGA AGGGCCAATC 
ATCAAAGAGA TCGGTGACCT CATGCTTTTG CAGTTTGATG GAGCTTCAGG GGAGGAAGTC 
CGAATGGAAG CAGCCAAGTT CTGCTCGTAC CAGACATCTG CCCTTGAGTT GATCAAAGCC 
AAAAAGAGGA AAGAGACCCG GTTCAGTCTC TTCATGCAGG AAGCTGAAAG CAACCCCCAG 
TGCCGACGAC TGCAGCTGAA GGACCTGATT GTGTCTGAGA TGCAACGGCT GACCAAGTAC 
CCTCTGCTGC TGGAAAACGT CCTTAAGCAC ACAGACTCCG GGACTCAGGA ACATGAAAAG 
CTGTACCGTT GCCGTGATCG GTGTCGAGAT ATTCTGAAAT ATGTCAACGA GTCCGTCAAA 
CAGGCAGAAA ATGAACATCG ACTGGCTGAG TATCAGAAGC GACTGGACGC AAGCCACTTG 
GAGCGGTCGA ATAATCCTCT AGCTGGAGAG TTTAAGAACT TAGACCTGAC CACAAGGCGA 
ATGATACATG AGGGGCCTCT CACCTGGAGG GTAACAAAAG ACAAGAATAT AGACTTGCAT 
GTTCTCTTGC TGGAAGATCT CCTCGTATTA CTTCAGAAGC AGGATGAGAA GCTGGTGCTC 
AAATGCCATA GCAAGACCGC CATGGTCTCC ACCGACACCA AACAAACCTT TAGTCCGGTC 
ATTAAGCTGA ATTCTGTCCT TGTTCGCTCG GTGGCTACAG ATAAAAGAGC CCTGTTTATT 
ATCTGCACTT CAGACTTGGG ACCTCAGATC TACGAGCTGG TGGCACTGAC ATCCTCAGAG 
AAAAACACGT GGAAGGATTT ATTGGAAGAA GCAACCAATG GTGCCATGAG GAATCCTCCC 
GGTGTCACCA AACGGAAATC CCAGGACATC AATAGACCAA CCTCTGCCAG CATGGTCCTA 
CAGAGAGCCC ACATGCCCAA AGTCTTGGCA GAAGGAGTTC TAAGACATAA CAGTGAGGAC 
CCTCAATCAG GTGATGAGAG CGCTGGGGAA TTCTTAGGCC ACCAAAAGCC AGATGTCGTC 
CTAGAGGAAT CGGAAAGGGA GGAAGATGAA GAAGATGAAG CTCGGACACC TACCCTGCTG 
CCTATCCCAT CTAAAGAGGA GGATGCCGGA GGTGGGACAC GGGGGTTAGA CTCTTCCAAC 
CATATTCCCT ACCAGTGGCC TGCAGTGACT GAAGGGCTGG CTGAGTCTGC ACTGGATGAT 
GTTCATAACC TCCGCCAGTT GATCTTGAAG AACATGCTCC ACCACGGCCA TTCCGAGTAC 
AACTCTTGCT CTGCCCCAGT GGCTGAGCCT GAAGATGACC TCACGCCTAC GCCTTCCATC 
ATTGGAGGAC ACGGGCACCC ATGGGACACT ATGACAGACG CAGTATTAGA AAATCTAGGC 
GGAGATCAGA CTACTGAAGA GAAGGAGACC TCTGTTCCAC CATCAGTTAG CAGCTCTGAG 
AGCAGCAGGT CTGTTTTGAA CTTTCCTCAT GGAGATAGAC CAGGTTCCAG AGATGGGGAG 
GAGACTGCTT GGAGTTTGGT TGAACGCTAC CCACAAAGCA GTTCTGTTCA TCAGAACCAA 
TCAGAGCAAC TACACTGTGA GGACCATAGT AACCAAAGCT ACAGAGTTGT TAGAACAGCT 
GAGGTGGTGG GAAATTATGA TGTCATACCG TTGCCAGACA GCAGCCAATC ACAGAGTGTG 
TTGCAGGAAG CTGGCGGTGG AGCTATTGTA GATGGAAATT TCTTTTATGT AACTGCGTCT 
ACGGCTTCTG CAGCTTCTGC AAAGGAAGAT AATGAAGTCT CAGAATCCCA AATGAACTCT 
GACCGGGCGG GCTTGTCCTT CTTGGAATCT CAAGAGTCAA ACCACACGCC AACTTACCAG 
CCAGAACTTG CCACCCCACC ATCAAGGAAT ACAGAAAGAG ATCCACTTTC ATCGGATCTC 
CTGCCCCCAA AACTTGTCAT TCAGGAAGTA GATATTATCT TCAGGACGAT AGAGCAGCTG 
ACGGCCAAAC TCCACAGGCT GAAGGTAGTA GAGTCAGCCC ATCAGGAGCT TCTGCGATCC 
TTAGGAAGAA GTGCCACAGA AGACCATTAC CATGAGAGAG AGCACATGGC AGAGAAGGAT 
AATCAAGCTC AGAAGCATCA GGGGTCCAAT TTGAACAGCT CCTCTCTATT CTTTGTGAAG 
AACAGCATAT CGGTCTCGCT TCATCGAGAT GGCAATACAG ACCCACAGGA AGATGCTATA 
AATCTGGCCA TGGGAATATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108700359 ( XM_018233483.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018088972.1 Xenopus laevis rho guanine nucleotide exchange factor 11-like (LOC108700359), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAf44535
Clone ID Related Accession (Same CDS sequence)	XM_018233483.1
Accession Version	XM_018233483.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4806bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1474214400000
Organism	Xenopus laevis(African clawed frog)
Product	rho guanine nucleotide exchange factor 11-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030739.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATCA GTTCCGCACA GACTTCAATA GACCGATCCG CTTCTAAACG CTATCCTCGT 
TTGCCAAGGT TGAGTAGCCT GTCATCGGCT AGTGACGGAT CTGCCGAGCG AAGGTCTCCT 
GGACACCAGC GTCAGCCTTG TAACGAGAGT AACGAGTCTA CAGGCCTGGT CCAGCGCTGT 
GTCATTGTTC AAAGGGACCA AAATGGATTT GGTTTCACGG TCAGCGGAGA TCGAATTGTT 
CTGGTTCAGT CTGTACGTCC AGGTGGGGCA GCAATGCGAG CTGGGGTGCA GGAGGGAGAC 
CGGATTGTGA AGGTCAACGG CACGCTGGTG GCTAACAGCT CACACATCGA AGTGGTCAAG 
CTGATTAAAT CCGGTGCTTA CGTGGCTCTC ACTCTCCTGG GTTCTCCACC TCCATCCATG 
GGCATGTCTG GGTATCAGCA AGACTTGAGC TCCTCTGTGT CCCCACGTAA CATATTAGTG 
TCGCCAATTC CTCCACCTCC ACCACCTTTG CCGCAGTCGC ATCACATCAC AGGACCTAAA 
CCCTTACAGG ACCTCGATTT CCAAAAGCAT GCTACGGAAA TTCTTTGGAA CATGTTAAAG 
CAGGAGGAAG CAGATCTACA GAATCTGTGT GATGTGTATA ACAGAAGCCC TAACCCCAGC 
CTGCAGGAGC AGATAGAGAG GGCCAGGAAG AGGGTGCATC AGCTGCAGCA TAAGATAAGG 
CCAGAAATAC CCAGTACTGG GAATTTGCTG CGTTCATACA GTGACTGCAG CTCTGTGGTG 
TATAAAGTCA AAGAAGGTCG CTTTTCTGTT GATTCTCAGG ATGGAGACAG TGGCCTGGAT 
TCGGGAACTG AACGGATATC ATCGTTGAGT GAGTATTCCA TGAATCGGAA TTCTGTCCTG 
TCAGACCTCG GAATGGACAG CTCCCACACC TCCCCAATCA TCCCAACGAA ACTGTTTCAC 
CACCACCATC GGCGGCACGG GTCAGACACC ACCTTTATTG CCACGTCTGA GCAGGTATCA 
GATCATAGGA GGTCTACGAT AATCGGGCCA GAAGAGGACT GTGACCATGG ATATTACATG 
ACAGAGCAGT CAGATGGTCT CTTCCAAGAT CTGGAAAAGT TAAAGACCCG GCCAGCCCAT 
ATGGGGGTGT TTCTACGTTA TATCTTCTCC CAGGCTGATC CAAACCCACT GCTCTTTTAC 
CTGAGTGCTG AGGCCTGTCA GCATTTGAGT ACAACAGACG CCTTCACATT GAGGAAAGAA 
GTCTGGAACA TCTTCCTTGA CAAAAGTGCA CCACTGAGGG TTAAAATCCC TGATCAGCTA 
CTGAATGAAA TTGAATCCCG TTTGCGGAAC TCGGAAGATA TCCGTGGTGT GTTTAGTGAA 
GCCCAGGAGG CGATGCTGCC AGAGATACAG GAGCAGATAC AGGATTACAG GACGAAAAGG 
ACCATGGGAT TAGGGAGCCT GTACGGGGAG AATGACCTGT TCGACCTTGA GATTGACCCT 
CACAGAGAAC GTCATGTTGC TGCTAAACAG ATTGCACAGC TGGAGGACAT ACTATCAAAG 
TATGAGAAAG AGATCAGCTC GCCCATGGCC TTTGCCTTGA GCATGTATGT GAAGTACGCT 
GGGATCCACA CTCAAGATAT CCGTCCCGCC AGCATCTCCG AAAAAAGCAT CCCCGACAGG 
GAAAAGTGGC TCCCGTTCTT CCCTAAGAAC AAGAAAAGCA GCAACGCCAA AAAGGATAAA 
GAGTCGGCTG AGGACAAGAA GCGCAACCCC ATCCTTAAGT ATATTAACAA GCCCAAGACA 
TTTCATGTTC CTTTGTCCCC TGGTGAAGTT AAACCCGGGA ATGTGAGAAA CATTATCCAG 
CACTTTGAAA ACAACCAACA ATATGATGTT CCCGAACTTA ACCTACAGCG GCTTTCAACC 
GGGAGCTTCC CTGAAGATCT ACTGGGCTCT GACAGCATCA GGTCGGAGGT GAAACTGGGC 
CGGTCAGAAA GCTTAAAGGG ACGGGAAGAG TTGAAGAAAT CCCGCAAATT GGACAATGTT 
CCTCGATCTC GCAGTGACGT AGATATGGAC GCGGCAGCTG AGGCTACACG GCTACACCAG 
TCGGCTTCAT CCTCTGCATC CAGCCTATCC GCAAGGTCAT TGGATAATCC TACCCCACCC 
TATACTCCCA AATTATGTCG AAGGAGCATG GAGTCGCCCA ATTTGGGGTT GACTGGAGAA 
TTGCTGCTCT CCAACCTGCA GGAGGACGAC CTGGGTCACC TCTCTGACCT TGACCCTGAG 
CCCGACGCAC AGAACTGGCA GCATACGGTG GGTCGGGACG TGGCGTTGCA CCTGCCACCC 
AAAGAGAGGG CACGGCAAGA GGTGATACAT GAGCTGTTTG TGACAGAAGC CTCCCACCTT 
CGGGTCCTAC GAGTTCTGGA TCTCGTTTTC TACCAGAAGA TGCGCAAGGA GAACCTGCTG 
ACCCCAGATG AGCTCTGGCT TGTCTTCCCC AATCTCCCAG AACTGATTGA GATCCACAAC 
TCGCTGGCAG AGTCAATGAA GCGGGTGAGA GAAGAAGGGC CAATCATCAA AGAGATCGGT 
GACCTCATGC TTTTGCAGTT TGATGGAGCT TCAGGGGAGG AAGTCCGAAT GGAAGCAGCC 
AAGTTCTGCT CGTACCAGAC ATCTGCCCTT GAGTTGATCA AAGCCAAAAA GAGGAAAGAG 
ACCCGGTTCA GTCTCTTCAT GCAGGAAGCT GAAAGCAACC CCCAGTGCCG ACGACTGCAG 
CTGAAGGACC TGATTGTGTC TGAGATGCAA CGGCTGACCA AGTACCCTCT GCTGCTGGAA 
AACGTCCTTA AGCACACAGA CTCCGGGACT CAGGAACATG AAAAGCTGTA CCGTTGCCGT 
GATCGGTGTC GAGATATTCT GAAATATGTC AACGAGTCCG TCAAACAGGC AGAAAATGAA 
CATCGACTGG CTGAGTATCA GAAGCGACTG GACGCAAGCC ACTTGGAGCG GTCGAATAAT 
CCTCTAGCTG GAGAGTTTAA GAACTTAGAC CTGACCACAA GGCGAATGAT ACATGAGGGG 
CCTCTCACCT GGAGGGTAAC AAAAGACAAG AATATAGACT TGCATGTTCT CTTGCTGGAA 
GATCTCCTCG TATTACTTCA GAAGCAGGAT GAGAAGCTGG TGCTCAAATG CCATAGCAAG 
ACCGCCATGG TCTCCACCGA CACCAAACAA ACCTTTAGTC CGGTCATTAA GCTGAATTCT 
GTCCTTGTTC GCTCGGTGGC TACAGATAAA AGAGCCCTGT TTATTATCTG CACTTCAGAC 
TTGGGACCTC AGATCTACGA GCTGGTGGCA CTGACATCCT CAGAGAAAAA CACGTGGAAG 
GATTTATTGG AAGAAGCAAC CAATGGTGCC ATGAGGAATC CTCCCGGTGT CACCAAACGG 
AAATCCCAGG ACATCAATAG ACCAACCTCT GCCAGCATGG TCCTACAGAG AGCCCACATG 
CCCAAAGTCT TGGCAGAAGG AGTTCTAAGA CATAACAGTG AGGACCCTCA ATCAGGTGAT 
GAGAGCGCTG GGGAATTCTT AGGCCACCAA AAGCCAGATG TCGTCCTAGA GGAATCGGAA 
AGGGAGGAAG ATGAAGAAGA TGAAGCTCGG ACACCTACCC TGCTGCCTAT CCCATCTAAA 
GAGGAGGATG CCGGAGGTGG GACACGGGGG TTAGACTCTT CCAACCATAT TCCCTACCAG 
TGGCCTGCAG TGACTGAAGG GCTGGCTGAG TCTGCACTGG ATGATGTTCA TAACCTCCGC 
CAGTTGATCT TGAAGAACAT GCTCCACCAC GGCCATTCCG AGTACAACTC TTGCTCTGCC 
CCAGTGGCTG AGCCTGAAGA TGACCTCACG CCTACGCCTT CCATCATTGG AGGACACGGG 
CACCCATGGG ACACTATGAC AGACGCAGTA TTAGAAAATC TAGGCGGAGA TCAGACTACT 
GAAGAGAAGG AGACCTCTGT TCCACCATCA GTTAGCAGCT CTGAGAGCAG CAGGTCTGTT 
TTGAACTTTC CTCATGGAGA TAGACCAGGT TCCAGAGATG GGGAGGAGAC TGCTTGGAGT 
TTGGTTGAAC GCTACCCACA AAGCAGTTCT GTTCATCAGA ACCAATCAGA GCAACTACAC 
TGTGAGGACC ATAGTAACCA AAGCTACAGA GTTGTTAGAA CAGCTGAGGT GGTGGGAAAT 
TATGATGTCA TACCGTTGCC AGACAGCAGC CAATCACAGA GTGTGTTGCA GGAAGCTGGC 
GGTGGAGCTA TTGTAGATGG AAATTTCTTT TATGTAACTG CGTCTACGGC TTCTGCAGCT 
TCTGCAAAGG AAGATAATGA AGTCTCAGAA TCCCAAATGA ACTCTGACCG GGCGGGCTTG 
TCCTTCTTGG AATCTCAAGA GTCAAACCAC ACGCCAACTT ACCAGCCAGA ACTTGCCACC 
CCACCATCAA GGAATACAGA AAGAGATCCA CTTTCATCGG ATCTCCTGCC CCCAAAACTT 
GTCATTCAGG AAGTAGATAT TATCTTCAGG ACGATAGAGC AGCTGACGGC CAAACTCCAC 
AGGCTGAAGG TAGTAGAGTC AGCCCATCAG GAGCTTCTGC GATCCTTAGG AAGAAGTGCC 
ACAGAAGACC ATTACCATGA GAGAGAGCAC ATGGCAGAGA AGGATAATCA AGCTCAGAAG 
CATCAGGGGT CCAATTTGAA CAGCTCCTCT CTATTCTTTG TGAAGAACAG CATATCGGTC 
TCGCTTCATC GAGATGGCAA TACAGACCCA CAGGAAGATG CTATAAATCT GGCCATGGGA 
ATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018088972.1
CDS	1131..5936
Translation	MSISSAQTSIDRSASKRYPRLPRLSSLSSASDGSAERRSPGHQRQPCNESNESTGLVQRCVIVQRDQNGFGFTVSGDRIVLVQSVRPGGAAMRAGVQEGDRIVKVNGTLVANSSHIEVVKLIKSGAYVALTLLGSPPPSMGMSGYQQDLSSSVSPRNILVSPIPPPPPPLPQSHHITGPKPLQDLDFQKHATEILWNMLKQEEADLQNLCDVYNRSPNPSLQEQIERARKRVHQLQHKIRPEIPSTGNLLRSYSDCSSVVYKVKEGRFSVDSQDGDSGLDSGTERISSLSEYSMNRNSVLSDLGMDSSHTSPIIPTKLFHHHHRRHGSDTTFIATSEQVSDHRRSTIIGPEEDCDHGYYMTEQSDGLFQDLEKLKTRPAHMGVFLRYIFSQADPNPLLFYLSAEACQHLSTTDAFTLRKEVWNIFLDKSAPLRVKIPDQLLNEIESRLRNSEDIRGVFSEAQEAMLPEIQEQIQDYRTKRTMGLGSLYGENDLFDLEIDPHRERHVAAKQIAQLEDILSKYEKEISSPMAFALSMYVKYAGIHTQDIRPASISEKSIPDREKWLPFFPKNKKSSNAKKDKESAEDKKRNPILKYINKPKTFHVPLSPGEVKPGNVRNIIQHFENNQQYDVPELNLQRLSTGSFPEDLLGSDSIRSEVKLGRSESLKGREELKKSRKLDNVPRSRSDVDMDAAAEATRLHQSASSSASSLSARSLDNPTPPYTPKLCRRSMESPNLGLTGELLLSNLQEDDLGHLSDLDPEPDAQNWQHTVGRDVALHLPPKERARQEVIHELFVTEASHLRVLRVLDLVFYQKMRKENLLTPDELWLVFPNLPELIEIHNSLAESMKRVREEGPIIKEIGDLMLLQFDGASGEEVRMEAAKFCSYQTSALELIKAKKRKETRFSLFMQEAESNPQCRRLQLKDLIVSEMQRLTKYPLLLENVLKHTDSGTQEHEKLYRCRDRCRDILKYVNESVKQAENEHRLAEYQKRLDASHLERSNNPLAGEFKNLDLTTRRMIHEGPLTWRVTKDKNIDLHVLLLEDLLVLLQKQDEKLVLKCHSKTAMVSTDTKQTFSPVIKLNSVLVRSVATDKRALFIICTSDLGPQIYELVALTSSEKNTWKDLLEEATNGAMRNPPGVTKRKSQDINRPTSASMVLQRAHMPKVLAEGVLRHNSEDPQSGDESAGEFLGHQKPDVVLEESEREEDEEDEARTPTLLPIPSKEEDAGGGTRGLDSSNHIPYQWPAVTEGLAESALDDVHNLRQLILKNMLHHGHSEYNSCSAPVAEPEDDLTPTPSIIGGHGHPWDTMTDAVLENLGGDQTTEEKETSVPPSVSSSESSRSVLNFPHGDRPGSRDGEETAWSLVERYPQSSSVHQNQSEQLHCEDHSNQSYRVVRTAEVVGNYDVIPLPDSSQSQSVLQEAGGGAIVDGNFFYVTASTASAASAKEDNEVSESQMNSDRAGLSFLESQESNHTPTYQPELATPPSRNTERDPLSSDLLPPKLVIQEVDIIFRTIEQLTAKLHRLKVVESAHQELLRSLGRSATEDHYHEREHMAEKDNQAQKHQGSNLNSSSLFFVKNSISVSLHRDGNTDPQEDAINLAMGI

Target ORF information:

RefSeq Version	XM_018233483.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis rho guanine nucleotide exchange factor 11-like (LOC108700359), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018233483.1

ORF Insert Sequence:

ATGAGCATCA GTTCCGCACA GACTTCAATA GACCGATCCG CTTCTAAACG CTATCCTCGT 
TTGCCAAGGT TGAGTAGCCT GTCATCGGCT AGTGACGGAT CTGCCGAGCG AAGGTCTCCT 
GGACACCAGC GTCAGCCTTG TAACGAGAGT AACGAGTCTA CAGGCCTGGT CCAGCGCTGT 
GTCATTGTTC AAAGGGACCA AAATGGATTT GGTTTCACGG TCAGCGGAGA TCGAATTGTT 
CTGGTTCAGT CTGTACGTCC AGGTGGGGCA GCAATGCGAG CTGGGGTGCA GGAGGGAGAC 
CGGATTGTGA AGGTCAACGG CACGCTGGTG GCTAACAGCT CACACATCGA AGTGGTCAAG 
CTGATTAAAT CCGGTGCTTA CGTGGCTCTC ACTCTCCTGG GTTCTCCACC TCCATCCATG 
GGCATGTCTG GGTATCAGCA AGACTTGAGC TCCTCTGTGT CCCCACGTAA CATATTAGTG 
TCGCCAATTC CTCCACCTCC ACCACCTTTG CCGCAGTCGC ATCACATCAC AGGACCTAAA 
CCCTTACAGG ACCTCGATTT CCAAAAGCAT GCTACGGAAA TTCTTTGGAA CATGTTAAAG 
CAGGAGGAAG CAGATCTACA GAATCTGTGT GATGTGTATA ACAGAAGCCC TAACCCCAGC 
CTGCAGGAGC AGATAGAGAG GGCCAGGAAG AGGGTGCATC AGCTGCAGCA TAAGATAAGG 
CCAGAAATAC CCAGTACTGG GAATTTGCTG CGTTCATACA GTGACTGCAG CTCTGTGGTG 
TATAAAGTCA AAGAAGGTCG CTTTTCTGTT GATTCTCAGG ATGGAGACAG TGGCCTGGAT 
TCGGGAACTG AACGGATATC ATCGTTGAGT GAGTATTCCA TGAATCGGAA TTCTGTCCTG 
TCAGACCTCG GAATGGACAG CTCCCACACC TCCCCAATCA TCCCAACGAA ACTGTTTCAC 
CACCACCATC GGCGGCACGG GTCAGACACC ACCTTTATTG CCACGTCTGA GCAGGTATCA 
GATCATAGGA GGTCTACGAT AATCGGGCCA GAAGAGGACT GTGACCATGG ATATTACATG 
ACAGAGCAGT CAGATGGTCT CTTCCAAGAT CTGGAAAAGT TAAAGACCCG GCCAGCCCAT 
ATGGGGGTGT TTCTACGTTA TATCTTCTCC CAGGCTGATC CAAACCCACT GCTCTTTTAC 
CTGAGTGCTG AGGCCTGTCA GCATTTGAGT ACAACAGACG CCTTCACATT GAGGAAAGAA 
GTCTGGAACA TCTTCCTTGA CAAAAGTGCA CCACTGAGGG TTAAAATCCC TGATCAGCTA 
CTGAATGAAA TTGAATCCCG TTTGCGGAAC TCGGAAGATA TCCGTGGTGT GTTTAGTGAA 
GCCCAGGAGG CGATGCTGCC AGAGATACAG GAGCAGATAC AGGATTACAG GACGAAAAGG 
ACCATGGGAT TAGGGAGCCT GTACGGGGAG AATGACCTGT TCGACCTTGA GATTGACCCT 
CACAGAGAAC GTCATGTTGC TGCTAAACAG ATTGCACAGC TGGAGGACAT ACTATCAAAG 
TATGAGAAAG AGATCAGCTC GCCCATGGCC TTTGCCTTGA GCATGTATGT GAAGTACGCT 
GGGATCCACA CTCAAGATAT CCGTCCCGCC AGCATCTCCG AAAAAAGCAT CCCCGACAGG 
GAAAAGTGGC TCCCGTTCTT CCCTAAGAAC AAGAAAAGCA GCAACGCCAA AAAGGATAAA 
GAGTCGGCTG AGGACAAGAA GCGCAACCCC ATCCTTAAGT ATATTAACAA GCCCAAGACA 
TTTCATGTTC CTTTGTCCCC TGGTGAAGTT AAACCCGGGA ATGTGAGAAA CATTATCCAG 
CACTTTGAAA ACAACCAACA ATATGATGTT CCCGAACTTA ACCTACAGCG GCTTTCAACC 
GGGAGCTTCC CTGAAGATCT ACTGGGCTCT GACAGCATCA GGTCGGAGGT GAAACTGGGC 
CGGTCAGAAA GCTTAAAGGG ACGGGAAGAG TTGAAGAAAT CCCGCAAATT GGACAATGTT 
CCTCGATCTC GCAGTGACGT AGATATGGAC GCGGCAGCTG AGGCTACACG GCTACACCAG 
TCGGCTTCAT CCTCTGCATC CAGCCTATCC GCAAGGTCAT TGGATAATCC TACCCCACCC 
TATACTCCCA AATTATGTCG AAGGAGCATG GAGTCGCCCA ATTTGGGGTT GACTGGAGAA 
TTGCTGCTCT CCAACCTGCA GGAGGACGAC CTGGGTCACC TCTCTGACCT TGACCCTGAG 
CCCGACGCAC AGAACTGGCA GCATACGGTG GGTCGGGACG TGGCGTTGCA CCTGCCACCC 
AAAGAGAGGG CACGGCAAGA GGTGATACAT GAGCTGTTTG TGACAGAAGC CTCCCACCTT 
CGGGTCCTAC GAGTTCTGGA TCTCGTTTTC TACCAGAAGA TGCGCAAGGA GAACCTGCTG 
ACCCCAGATG AGCTCTGGCT TGTCTTCCCC AATCTCCCAG AACTGATTGA GATCCACAAC 
TCGCTGGCAG AGTCAATGAA GCGGGTGAGA GAAGAAGGGC CAATCATCAA AGAGATCGGT 
GACCTCATGC TTTTGCAGTT TGATGGAGCT TCAGGGGAGG AAGTCCGAAT GGAAGCAGCC 
AAGTTCTGCT CGTACCAGAC ATCTGCCCTT GAGTTGATCA AAGCCAAAAA GAGGAAAGAG 
ACCCGGTTCA GTCTCTTCAT GCAGGAAGCT GAAAGCAACC CCCAGTGCCG ACGACTGCAG 
CTGAAGGACC TGATTGTGTC TGAGATGCAA CGGCTGACCA AGTACCCTCT GCTGCTGGAA 
AACGTCCTTA AGCACACAGA CTCCGGGACT CAGGAACATG AAAAGCTGTA CCGTTGCCGT 
GATCGGTGTC GAGATATTCT GAAATATGTC AACGAGTCCG TCAAACAGGC AGAAAATGAA 
CATCGACTGG CTGAGTATCA GAAGCGACTG GACGCAAGCC ACTTGGAGCG GTCGAATAAT 
CCTCTAGCTG GAGAGTTTAA GAACTTAGAC CTGACCACAA GGCGAATGAT ACATGAGGGG 
CCTCTCACCT GGAGGGTAAC AAAAGACAAG AATATAGACT TGCATGTTCT CTTGCTGGAA 
GATCTCCTCG TATTACTTCA GAAGCAGGAT GAGAAGCTGG TGCTCAAATG CCATAGCAAG 
ACCGCCATGG TCTCCACCGA CACCAAACAA ACCTTTAGTC CGGTCATTAA GCTGAATTCT 
GTCCTTGTTC GCTCGGTGGC TACAGATAAA AGAGCCCTGT TTATTATCTG CACTTCAGAC 
TTGGGACCTC AGATCTACGA GCTGGTGGCA CTGACATCCT CAGAGAAAAA CACGTGGAAG 
GATTTATTGG AAGAAGCAAC CAATGGTGCC ATGAGGAATC CTCCCGGTGT CACCAAACGG 
AAATCCCAGG ACATCAATAG ACCAACCTCT GCCAGCATGG TCCTACAGAG AGCCCACATG 
CCCAAAGTCT TGGCAGAAGG AGTTCTAAGA CATAACAGTG AGGACCCTCA ATCAGGTGAT 
GAGAGCGCTG GGGAATTCTT AGGCCACCAA AAGCCAGATG TCGTCCTAGA GGAATCGGAA 
AGGGAGGAAG ATGAAGAAGA TGAAGCTCGG ACACCTACCC TGCTGCCTAT CCCATCTAAA 
GAGGAGGATG CCGGAGGTGG GACACGGGGG TTAGACTCTT CCAACCATAT TCCCTACCAG 
TGGCCTGCAG TGACTGAAGG GCTGGCTGAG TCTGCACTGG ATGATGTTCA TAACCTCCGC 
CAGTTGATCT TGAAGAACAT GCTCCACCAC GGCCATTCCG AGTACAACTC TTGCTCTGCC 
CCAGTGGCTG AGCCTGAAGA TGACCTCACG CCTACGCCTT CCATCATTGG AGGACACGGG 
CACCCATGGG ACACTATGAC AGACGCAGTA TTAGAAAATC TAGGCGGAGA TCAGACTACT 
GAAGAGAAGG AGACCTCTGT TCCACCATCA GTTAGCAGCT CTGAGAGCAG CAGGTCTGTT 
TTGAACTTTC CTCATGGAGA TAGACCAGGT TCCAGAGATG GGGAGGAGAC TGCTTGGAGT 
TTGGTTGAAC GCTACCCACA AAGCAGTTCT GTTCATCAGA ACCAATCAGA GCAACTACAC 
TGTGAGGACC ATAGTAACCA AAGCTACAGA GTTGTTAGAA CAGCTGAGGT GGTGGGAAAT 
TATGATGTCA TACCGTTGCC AGACAGCAGC CAATCACAGA GTGTGTTGCA GGAAGCTGGC 
GGTGGAGCTA TTGTAGATGG AAATTTCTTT TATGTAACTG CGTCTACGGC TTCTGCAGCT 
TCTGCAAAGG AAGATAATGA AGTCTCAGAA TCCCAAATGA ACTCTGACCG GGCGGGCTTG 
TCCTTCTTGG AATCTCAAGA GTCAAACCAC ACGCCAACTT ACCAGCCAGA ACTTGCCACC 
CCACCATCAA GGAATACAGA AAGAGATCCA CTTTCATCGG ATCTCCTGCC CCCAAAACTT 
GTCATTCAGG AAGTAGATAT TATCTTCAGG ACGATAGAGC AGCTGACGGC CAAACTCCAC 
AGGCTGAAGG TAGTAGAGTC AGCCCATCAG GAGCTTCTGC GATCCTTAGG AAGAAGTGCC 
ACAGAAGACC ATTACCATGA GAGAGAGCAC ATGGCAGAGA AGGATAATCA AGCTCAGAAG 
CATCAGGGGT CCAATTTGAA CAGCTCCTCT CTATTCTTTG TGAAGAACAG CATATCGGTC 
TCGCTTCATC GAGATGGCAA TACAGACCCA CAGGAAGATG CTATAAATCT GGCCATGGGA 
ATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108700359 ( XM_018233484.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018088973.1 Xenopus laevis rho guanine nucleotide exchange factor 11-like (LOC108700359), transcript variant X3, mRNA.

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CloneID	OAf44536
Clone ID Related Accession (Same CDS sequence)	XM_018233484.1
Accession Version	XM_018233484.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1474214400000
Organism	Xenopus laevis(African clawed frog)
Product	rho guanine nucleotide exchange factor 11-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030739.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATCA GTTCCGCACA GACTTCAATA GACCGATCCG CTTCTAAACG CTATCCTCGT 
TTGCCAAGGT TGAGTAGCCT GTCATCGGCT AGTGACGGAT CTGCCGAGCG AAGGTCTCCT 
GGACACCAGC GTCAGCCTTG TAACGAGAGT AACGAGTCTA CAGGCCTGGT CCAGCGCTGT 
GTCATTGTTC AAAGGGACCA AAATGGATTT GGTTTCACGG TCAGCGGAGA TCGAATTGTT 
CTGGTTCAGT CTGTACGTCC AGGTGGGGCA GCAATGCGAG CTGGGGTGCA GGAGGGAGAC 
CGGATTGTGA AGGTCAACGG CACGCTGGTG GCTAACAGCT CACACATCGA AGTGGTCAAG 
CTGATTAAAT CCGGTGCTTA CGTGGCTCTC ACTCTCCTGG GTTCTCCACC TCCATCCATG 
GGCATGTCTG GGTATCAGCA AGACTTGAGC TCCTCTGTGT CCCCACGTAA CATATTAGTG 
TCGCCAATTC CTCCACCTCC ACCACCTTTG CCGCAGTCGC ATCACATCAC AGGACCTAAA 
CCCTTACAGG ACCTCGATTT CCAAAAGCAT GCTACGGAAA TTCTTTGGAA CATGTTAAAG 
CAGGAGGAAG CAGATCTACA GAATCTGTGT GATGTGTATA ACAGAAGCCC TAACCCCAGC 
CTGCAGGAGC AGATAGAGAG GGCCAGGAAG AGGGTGCATC AGCTGCAGCA TAAGATAAGG 
CCAGAAATAC CCAGTACTGG GAATTTGCTG CGTTCATACA GTGACTGCAG CTCTGTGGTG 
TATAAAGTCA AAGAAGGTCG CTTTTCTGTT GATTCTCAGG ATGGAGACAG TGGCCTGGAT 
TCGGGAACTG AACGGATATC ATCGTTGAGT GAGTATTCCA TGAATCGGAA TTCTGTCCTG 
TCAGACCTCG GAATGGACAG CTCCCACACC TCCCCAATCA TCCCAACGAA ACTGTTTCAC 
CACCACCATC GGCGGCACGG GTCAGACACC ACCTTTATTG CCACGTCTGA GCAGGTATCA 
GATCATAGGA GGTCTACGAT AATCGGGCCA GAAGAGGACT GTGACCATGG ATATTACATG 
ACAGAGCAGT CAGATGGTCT CTTCCAAGAT CTGGAAAAGT TAAAGACCCG GCCAGCCCAT 
ATGGGGGTGT TTCTACGTTA TATCTTCTCC CAGGCTGATC CAAACCCACT GCTCTTTTAC 
CTGAGTGCTG AGGCCTGTCA GCATTTGAGT ACAACAGACG CCTTCACATT GAGGAAAGAA 
GTCTGGAACA TCTTCCTTGA CAAAAGTGCA CCACTGAGGG TTAAAATCCC TGATCAGCTA 
CTGAATGAAA TTGAATCCCG TTTGCGGAAC TCGGAAGATA TCCGTGGTGT GTTTAGTGAA 
GCCCAGGAGG CGATGCTGCC AGAGATACAG GAGCAGATAC AGGATTACAG GACGAAAAGG 
ACCATGGGAT TAGGGAGCCT GTACGGGGAG AATGACCTGT TCGACCTTGA GATTGACCCT 
CACAGAGAAC GTCATGTTGC TGCTAAACAG ATTGCACAGC TGGAGGACAT ACTATCAAAG 
TATGAGAAAG AGATCAGCTC GCCCATGGCC TTTGCCTTGA GCATGTATGT GAAGTACGCT 
GGGATCCACA CTCAAGATAT CCGTCCCGCC AGCATCTCCG AAAAAAGCAT CCCCGACAGG 
GAAAAGTGGC TCCCGTTCTT CCCTAAGAAC AAGAAAAGCA GCAACGCCAA AAAGGATAAA 
GAGTCGGCTG AGGACAAGAA GCGCAACCCC ATCCTTAAGT ATATTAACAA GCCCAAGAGT 
ACGTCTCAGA GTATTAAACC CGGGAATGTG AGAAACATTA TCCAGCACTT TGAAAACAAC 
CAACAATATG ATGTTCCCGA ACTTAACCTA CAGCGGCTTT CAACCGGGAG CTTCCCTGAA 
GATCTACTGG GCTCTGACAG CATCAGGTCG GAGGTGAAAC TGGGCCGGTC AGAAAGCTTA 
AAGGGACGGG AAGAGTTGAA GAAATCCCGC AAATTGGACA ATGTTCCTCG ATCTCGCAGT 
GACGTAGATA TGGACGCGGC AGCTGAGGCT ACACGGCTAC ACCAGTCGGC TTCATCCTCT 
GCATCCAGCC TATCCGCAAG GTCATTGGAT AATCCTACCC CACCCTATAC TCCCAAATTA 
TGTCGAAGGA GCATGGAGTC GCCCAATTTG GGGTTGACTG GAGAATTGCT GCTCTCCAAC 
CTGCAGGAGG ACGACCTGGG TCACCTCTCT GACCTTGACC CTGAGCCCGA CGCACAGAAC 
TGGCAGCATA CGGTGGGTCG GGACGTGGCG TTGCACCTGC CACCCAAAGA GAGGGCACGG 
CAAGAGGTGA TACATGAGCT GTTTGTGACA GAAGCCTCCC ACCTTCGGGT CCTACGAGTT 
CTGGATCTCG TTTTCTACCA GAAGATGCGC AAGGAGAACC TGCTGACCCC AGATGAGCTC 
TGGCTTGTCT TCCCCAATCT CCCAGAACTG ATTGAGATCC ACAACTCGCT GGCAGAGTCA 
ATGAAGCGGG TGAGAGAAGA AGGGCCAATC ATCAAAGAGA TCGGTGACCT CATGCTTTTG 
CAGTTTGATG GAGCTTCAGG GGAGGAAGTC CGAATGGAAG CAGCCAAGTT CTGCTCGTAC 
CAGACATCTG CCCTTGAGTT GATCAAAGCC AAAAAGAGGA AAGAGACCCG GTTCAGTCTC 
TTCATGCAGG AAGCTGAAAG CAACCCCCAG TGCCGACGAC TGCAGCTGAA GGACCTGATT 
GTGTCTGAGA TGCAACGGCT GACCAAGTAC CCTCTGCTGC TGGAAAACGT CCTTAAGCAC 
ACAGACTCCG GGACTCAGGA ACATGAAAAG CTGTACCGTT GCCGTGATCG GTGTCGAGAT 
ATTCTGAAAT ATGTCAACGA GTCCGTCAAA CAGGCAGAAA ATGAACATCG ACTGGCTGAG 
TATCAGAAGC GACTGGACGC AAGCCACTTG GAGCGGTCGA ATAATCCTCT AGCTGGAGAG 
TTTAAGAACT TAGACCTGAC CACAAGGCGA ATGATACATG AGGGGCCTCT CACCTGGAGG 
GTAACAAAAG ACAAGAATAT AGACTTGCAT GTTCTCTTGC TGGAAGATCT CCTCGTATTA 
CTTCAGAAGC AGGATGAGAA GCTGGTGCTC AAATGCCATA GCAAGACCGC CATGGTCTCC 
ACCGACACCA AACAAACCTT TAGTCCGGTC ATTAAGCTGA ATTCTGTCCT TGTTCGCTCG 
GTGGCTACAG ATAAAAGAGC CCTGTTTATT ATCTGCACTT CAGACTTGGG ACCTCAGATC 
TACGAGCTGG TGGCACTGAC ATCCTCAGAG AAAAACACGT GGAAGGATTT ATTGGAAGAA 
GCAACCAATG GTGCCATGAG GAATCCTCCC GGTGTCACCA AACGGAAATC CCAGGACATC 
AATAGACCAA CCTCTGCCAG CATGGTCCTA CAGAGAGCCC ACATGCCCAA AGTCTTGGCA 
GAAGGAGTTC TAAGACATAA CAGTGAGGAC CCTCAATCAG GTGATGAGAG CGCTGGGGAA 
TTCTTAGGCC ACCAAAAGCC AGATGTCGTC CTAGAGGAAT CGGAAAGGGA GGAAGATGAA 
GAAGATGAAG CTCGGACACC TACCCTGCTG CCTATCCCAT CTAAAGAGGA GGATGCCGGA 
GGTGGGACAC GGGGGTTAGA CTCTTCCAAC CATATTCCCT ACCAGTGGCC TGCAGTGACT 
GAAGGGCTGG CTGAGTCTGC ACTGGATGAT GTTCATAACC TCCGCCAGTT GATCTTGAAG 
AACATGCTCC ACCACGGCCA TTCCGAGTAC AACTCTTGCT CTGCCCCAGT GGCTGAGCCT 
GAAGATGACC TCACGCCTAC GCCTTCCATC ATTGGAGGAC ACGGGCACCC ATGGGACACT 
ATGACAGACG CAGTATTAGA AAATCTAGGC GGAGATCAGA CTACTGAAGA GAAGGAGACC 
TCTGTTCCAC CATCAGTTAG CAGCTCTGAG AGCAGCAGGT CTGTTTTGAA CTTTCCTCAT 
GGAGATAGAC CAGGTTCCAG AGATGGGGAG GAGACTGCTT GGAGTTTGGT TGAACGCTAC 
CCACAAAGCA GTTCTGTTCA TCAGAACCAA TCAGAGCAAC TACACTGTGA GGACCATAGT 
AACCAAAGCT ACAGAGTTGT TAGAACAGCT GAGGTGGTGG GAAATTATGA TGTCATACCG 
TTGCCAGACA GCAGCCAATC ACAGAGTGTG TTGCAGGAAG CTGGCGGTGG AGCTATTGTA 
GATGGAAATT TCTTTTATGT AACTGCGTCT ACGGCTTCTG CAGCTTCTGC AAAGGAAGAT 
AATGAAGTCT CAGAATCCCA AATGAACTCT GACCGGGCGG GCTTGTCCTT CTTGGAATCT 
CAAGAGTCAA ACCACACGCC AACTTACCAG CCAGAACTTG CCACCCCACC ATCAAGGAAT 
ACAGAAAGAG ATCCACTTTC ATCGGATCTC CTGCCCCCAA AACTTGTCAT TCAGGAAGTA 
GATATTATCT TCAGGACGAT AGAGCAGCTG ACGGCCAAAC TCCACAGGCT GAAGGTAGTA 
GAGTCAGCCC ATCAGGAGCT TCTGCGATCC TTAGGAAGAA GTGCCACAGA AGACCATTAC 
CATGAGAGAG AGCACATGGC AGAGAAGGAT AATCAAGCTC AGAAGCATCA GGGGTCCAAT 
TTGAACAGCT CCTCTCTATT CTTTGTGAAG AACAGCATAT CGGTCTCGCT TCATCGAGAT 
GGCAATACAG ACCCACAGGA AGATGCTATA AATCTGGCCA TGGGAATATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018088973.1
CDS	1131..5921
Translation	MSISSAQTSIDRSASKRYPRLPRLSSLSSASDGSAERRSPGHQRQPCNESNESTGLVQRCVIVQRDQNGFGFTVSGDRIVLVQSVRPGGAAMRAGVQEGDRIVKVNGTLVANSSHIEVVKLIKSGAYVALTLLGSPPPSMGMSGYQQDLSSSVSPRNILVSPIPPPPPPLPQSHHITGPKPLQDLDFQKHATEILWNMLKQEEADLQNLCDVYNRSPNPSLQEQIERARKRVHQLQHKIRPEIPSTGNLLRSYSDCSSVVYKVKEGRFSVDSQDGDSGLDSGTERISSLSEYSMNRNSVLSDLGMDSSHTSPIIPTKLFHHHHRRHGSDTTFIATSEQVSDHRRSTIIGPEEDCDHGYYMTEQSDGLFQDLEKLKTRPAHMGVFLRYIFSQADPNPLLFYLSAEACQHLSTTDAFTLRKEVWNIFLDKSAPLRVKIPDQLLNEIESRLRNSEDIRGVFSEAQEAMLPEIQEQIQDYRTKRTMGLGSLYGENDLFDLEIDPHRERHVAAKQIAQLEDILSKYEKEISSPMAFALSMYVKYAGIHTQDIRPASISEKSIPDREKWLPFFPKNKKSSNAKKDKESAEDKKRNPILKYINKPKSTSQSIKPGNVRNIIQHFENNQQYDVPELNLQRLSTGSFPEDLLGSDSIRSEVKLGRSESLKGREELKKSRKLDNVPRSRSDVDMDAAAEATRLHQSASSSASSLSARSLDNPTPPYTPKLCRRSMESPNLGLTGELLLSNLQEDDLGHLSDLDPEPDAQNWQHTVGRDVALHLPPKERARQEVIHELFVTEASHLRVLRVLDLVFYQKMRKENLLTPDELWLVFPNLPELIEIHNSLAESMKRVREEGPIIKEIGDLMLLQFDGASGEEVRMEAAKFCSYQTSALELIKAKKRKETRFSLFMQEAESNPQCRRLQLKDLIVSEMQRLTKYPLLLENVLKHTDSGTQEHEKLYRCRDRCRDILKYVNESVKQAENEHRLAEYQKRLDASHLERSNNPLAGEFKNLDLTTRRMIHEGPLTWRVTKDKNIDLHVLLLEDLLVLLQKQDEKLVLKCHSKTAMVSTDTKQTFSPVIKLNSVLVRSVATDKRALFIICTSDLGPQIYELVALTSSEKNTWKDLLEEATNGAMRNPPGVTKRKSQDINRPTSASMVLQRAHMPKVLAEGVLRHNSEDPQSGDESAGEFLGHQKPDVVLEESEREEDEEDEARTPTLLPIPSKEEDAGGGTRGLDSSNHIPYQWPAVTEGLAESALDDVHNLRQLILKNMLHHGHSEYNSCSAPVAEPEDDLTPTPSIIGGHGHPWDTMTDAVLENLGGDQTTEEKETSVPPSVSSSESSRSVLNFPHGDRPGSRDGEETAWSLVERYPQSSSVHQNQSEQLHCEDHSNQSYRVVRTAEVVGNYDVIPLPDSSQSQSVLQEAGGGAIVDGNFFYVTASTASAASAKEDNEVSESQMNSDRAGLSFLESQESNHTPTYQPELATPPSRNTERDPLSSDLLPPKLVIQEVDIIFRTIEQLTAKLHRLKVVESAHQELLRSLGRSATEDHYHEREHMAEKDNQAQKHQGSNLNSSSLFFVKNSISVSLHRDGNTDPQEDAINLAMGI

Target ORF information:

RefSeq Version	XM_018233484.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis rho guanine nucleotide exchange factor 11-like (LOC108700359), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018233484.1

ORF Insert Sequence:

ATGAGCATCA GTTCCGCACA GACTTCAATA GACCGATCCG CTTCTAAACG CTATCCTCGT 
TTGCCAAGGT TGAGTAGCCT GTCATCGGCT AGTGACGGAT CTGCCGAGCG AAGGTCTCCT 
GGACACCAGC GTCAGCCTTG TAACGAGAGT AACGAGTCTA CAGGCCTGGT CCAGCGCTGT 
GTCATTGTTC AAAGGGACCA AAATGGATTT GGTTTCACGG TCAGCGGAGA TCGAATTGTT 
CTGGTTCAGT CTGTACGTCC AGGTGGGGCA GCAATGCGAG CTGGGGTGCA GGAGGGAGAC 
CGGATTGTGA AGGTCAACGG CACGCTGGTG GCTAACAGCT CACACATCGA AGTGGTCAAG 
CTGATTAAAT CCGGTGCTTA CGTGGCTCTC ACTCTCCTGG GTTCTCCACC TCCATCCATG 
GGCATGTCTG GGTATCAGCA AGACTTGAGC TCCTCTGTGT CCCCACGTAA CATATTAGTG 
TCGCCAATTC CTCCACCTCC ACCACCTTTG CCGCAGTCGC ATCACATCAC AGGACCTAAA 
CCCTTACAGG ACCTCGATTT CCAAAAGCAT GCTACGGAAA TTCTTTGGAA CATGTTAAAG 
CAGGAGGAAG CAGATCTACA GAATCTGTGT GATGTGTATA ACAGAAGCCC TAACCCCAGC 
CTGCAGGAGC AGATAGAGAG GGCCAGGAAG AGGGTGCATC AGCTGCAGCA TAAGATAAGG 
CCAGAAATAC CCAGTACTGG GAATTTGCTG CGTTCATACA GTGACTGCAG CTCTGTGGTG 
TATAAAGTCA AAGAAGGTCG CTTTTCTGTT GATTCTCAGG ATGGAGACAG TGGCCTGGAT 
TCGGGAACTG AACGGATATC ATCGTTGAGT GAGTATTCCA TGAATCGGAA TTCTGTCCTG 
TCAGACCTCG GAATGGACAG CTCCCACACC TCCCCAATCA TCCCAACGAA ACTGTTTCAC 
CACCACCATC GGCGGCACGG GTCAGACACC ACCTTTATTG CCACGTCTGA GCAGGTATCA 
GATCATAGGA GGTCTACGAT AATCGGGCCA GAAGAGGACT GTGACCATGG ATATTACATG 
ACAGAGCAGT CAGATGGTCT CTTCCAAGAT CTGGAAAAGT TAAAGACCCG GCCAGCCCAT 
ATGGGGGTGT TTCTACGTTA TATCTTCTCC CAGGCTGATC CAAACCCACT GCTCTTTTAC 
CTGAGTGCTG AGGCCTGTCA GCATTTGAGT ACAACAGACG CCTTCACATT GAGGAAAGAA 
GTCTGGAACA TCTTCCTTGA CAAAAGTGCA CCACTGAGGG TTAAAATCCC TGATCAGCTA 
CTGAATGAAA TTGAATCCCG TTTGCGGAAC TCGGAAGATA TCCGTGGTGT GTTTAGTGAA 
GCCCAGGAGG CGATGCTGCC AGAGATACAG GAGCAGATAC AGGATTACAG GACGAAAAGG 
ACCATGGGAT TAGGGAGCCT GTACGGGGAG AATGACCTGT TCGACCTTGA GATTGACCCT 
CACAGAGAAC GTCATGTTGC TGCTAAACAG ATTGCACAGC TGGAGGACAT ACTATCAAAG 
TATGAGAAAG AGATCAGCTC GCCCATGGCC TTTGCCTTGA GCATGTATGT GAAGTACGCT 
GGGATCCACA CTCAAGATAT CCGTCCCGCC AGCATCTCCG AAAAAAGCAT CCCCGACAGG 
GAAAAGTGGC TCCCGTTCTT CCCTAAGAAC AAGAAAAGCA GCAACGCCAA AAAGGATAAA 
GAGTCGGCTG AGGACAAGAA GCGCAACCCC ATCCTTAAGT ATATTAACAA GCCCAAGAGT 
ACGTCTCAGA GTATTAAACC CGGGAATGTG AGAAACATTA TCCAGCACTT TGAAAACAAC 
CAACAATATG ATGTTCCCGA ACTTAACCTA CAGCGGCTTT CAACCGGGAG CTTCCCTGAA 
GATCTACTGG GCTCTGACAG CATCAGGTCG GAGGTGAAAC TGGGCCGGTC AGAAAGCTTA 
AAGGGACGGG AAGAGTTGAA GAAATCCCGC AAATTGGACA ATGTTCCTCG ATCTCGCAGT 
GACGTAGATA TGGACGCGGC AGCTGAGGCT ACACGGCTAC ACCAGTCGGC TTCATCCTCT 
GCATCCAGCC TATCCGCAAG GTCATTGGAT AATCCTACCC CACCCTATAC TCCCAAATTA 
TGTCGAAGGA GCATGGAGTC GCCCAATTTG GGGTTGACTG GAGAATTGCT GCTCTCCAAC 
CTGCAGGAGG ACGACCTGGG TCACCTCTCT GACCTTGACC CTGAGCCCGA CGCACAGAAC 
TGGCAGCATA CGGTGGGTCG GGACGTGGCG TTGCACCTGC CACCCAAAGA GAGGGCACGG 
CAAGAGGTGA TACATGAGCT GTTTGTGACA GAAGCCTCCC ACCTTCGGGT CCTACGAGTT 
CTGGATCTCG TTTTCTACCA GAAGATGCGC AAGGAGAACC TGCTGACCCC AGATGAGCTC 
TGGCTTGTCT TCCCCAATCT CCCAGAACTG ATTGAGATCC ACAACTCGCT GGCAGAGTCA 
ATGAAGCGGG TGAGAGAAGA AGGGCCAATC ATCAAAGAGA TCGGTGACCT CATGCTTTTG 
CAGTTTGATG GAGCTTCAGG GGAGGAAGTC CGAATGGAAG CAGCCAAGTT CTGCTCGTAC 
CAGACATCTG CCCTTGAGTT GATCAAAGCC AAAAAGAGGA AAGAGACCCG GTTCAGTCTC 
TTCATGCAGG AAGCTGAAAG CAACCCCCAG TGCCGACGAC TGCAGCTGAA GGACCTGATT 
GTGTCTGAGA TGCAACGGCT GACCAAGTAC CCTCTGCTGC TGGAAAACGT CCTTAAGCAC 
ACAGACTCCG GGACTCAGGA ACATGAAAAG CTGTACCGTT GCCGTGATCG GTGTCGAGAT 
ATTCTGAAAT ATGTCAACGA GTCCGTCAAA CAGGCAGAAA ATGAACATCG ACTGGCTGAG 
TATCAGAAGC GACTGGACGC AAGCCACTTG GAGCGGTCGA ATAATCCTCT AGCTGGAGAG 
TTTAAGAACT TAGACCTGAC CACAAGGCGA ATGATACATG AGGGGCCTCT CACCTGGAGG 
GTAACAAAAG ACAAGAATAT AGACTTGCAT GTTCTCTTGC TGGAAGATCT CCTCGTATTA 
CTTCAGAAGC AGGATGAGAA GCTGGTGCTC AAATGCCATA GCAAGACCGC CATGGTCTCC 
ACCGACACCA AACAAACCTT TAGTCCGGTC ATTAAGCTGA ATTCTGTCCT TGTTCGCTCG 
GTGGCTACAG ATAAAAGAGC CCTGTTTATT ATCTGCACTT CAGACTTGGG ACCTCAGATC 
TACGAGCTGG TGGCACTGAC ATCCTCAGAG AAAAACACGT GGAAGGATTT ATTGGAAGAA 
GCAACCAATG GTGCCATGAG GAATCCTCCC GGTGTCACCA AACGGAAATC CCAGGACATC 
AATAGACCAA CCTCTGCCAG CATGGTCCTA CAGAGAGCCC ACATGCCCAA AGTCTTGGCA 
GAAGGAGTTC TAAGACATAA CAGTGAGGAC CCTCAATCAG GTGATGAGAG CGCTGGGGAA 
TTCTTAGGCC ACCAAAAGCC AGATGTCGTC CTAGAGGAAT CGGAAAGGGA GGAAGATGAA 
GAAGATGAAG CTCGGACACC TACCCTGCTG CCTATCCCAT CTAAAGAGGA GGATGCCGGA 
GGTGGGACAC GGGGGTTAGA CTCTTCCAAC CATATTCCCT ACCAGTGGCC TGCAGTGACT 
GAAGGGCTGG CTGAGTCTGC ACTGGATGAT GTTCATAACC TCCGCCAGTT GATCTTGAAG 
AACATGCTCC ACCACGGCCA TTCCGAGTAC AACTCTTGCT CTGCCCCAGT GGCTGAGCCT 
GAAGATGACC TCACGCCTAC GCCTTCCATC ATTGGAGGAC ACGGGCACCC ATGGGACACT 
ATGACAGACG CAGTATTAGA AAATCTAGGC GGAGATCAGA CTACTGAAGA GAAGGAGACC 
TCTGTTCCAC CATCAGTTAG CAGCTCTGAG AGCAGCAGGT CTGTTTTGAA CTTTCCTCAT 
GGAGATAGAC CAGGTTCCAG AGATGGGGAG GAGACTGCTT GGAGTTTGGT TGAACGCTAC 
CCACAAAGCA GTTCTGTTCA TCAGAACCAA TCAGAGCAAC TACACTGTGA GGACCATAGT 
AACCAAAGCT ACAGAGTTGT TAGAACAGCT GAGGTGGTGG GAAATTATGA TGTCATACCG 
TTGCCAGACA GCAGCCAATC ACAGAGTGTG TTGCAGGAAG CTGGCGGTGG AGCTATTGTA 
GATGGAAATT TCTTTTATGT AACTGCGTCT ACGGCTTCTG CAGCTTCTGC AAAGGAAGAT 
AATGAAGTCT CAGAATCCCA AATGAACTCT GACCGGGCGG GCTTGTCCTT CTTGGAATCT 
CAAGAGTCAA ACCACACGCC AACTTACCAG CCAGAACTTG CCACCCCACC ATCAAGGAAT 
ACAGAAAGAG ATCCACTTTC ATCGGATCTC CTGCCCCCAA AACTTGTCAT TCAGGAAGTA 
GATATTATCT TCAGGACGAT AGAGCAGCTG ACGGCCAAAC TCCACAGGCT GAAGGTAGTA 
GAGTCAGCCC ATCAGGAGCT TCTGCGATCC TTAGGAAGAA GTGCCACAGA AGACCATTAC 
CATGAGAGAG AGCACATGGC AGAGAAGGAT AATCAAGCTC AGAAGCATCA GGGGTCCAAT 
TTGAACAGCT CCTCTCTATT CTTTGTGAAG AACAGCATAT CGGTCTCGCT TCATCGAGAT 
GGCAATACAG ACCCACAGGA AGATGCTATA AATCTGGCCA TGGGAATATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108700359 ( XM_018233485.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018088974.1 Xenopus laevis rho guanine nucleotide exchange factor 11-like (LOC108700359), transcript variant X4, mRNA.

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CloneID	OAf44537
Clone ID Related Accession (Same CDS sequence)	XM_018233485.1
Accession Version	XM_018233485.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4788bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1474214400000
Organism	Xenopus laevis(African clawed frog)
Product	rho guanine nucleotide exchange factor 11-like isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030739.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATCA GTTCCGCACA GACTTCAATA GACCGGTTGA GTAGCCTGTC ATCGGCTAGT 
GACGGATCTG CCGAGCGAAG GTCTCCTGGA CACCAGCGTC AGCCTTGTAA CGAGAGTAAC 
GAGTCTACAG GCCTGGTCCA GCGCTGTGTC ATTGTTCAAA GGGACCAAAA TGGATTTGGT 
TTCACGGTCA GCGGAGATCG AATTGTTCTG GTTCAGTCTG TACGTCCAGG TGGGGCAGCA 
ATGCGAGCTG GGGTGCAGGA GGGAGACCGG ATTGTGAAGG TCAACGGCAC GCTGGTGGCT 
AACAGCTCAC ACATCGAAGT GGTCAAGCTG ATTAAATCCG GTGCTTACGT GGCTCTCACT 
CTCCTGGGTT CTCCACCTCC ATCCATGGGC ATGTCTGGGT ATCAGCAAGA CTTGAGCTCC 
TCTGTGTCCC CACGTAACAT ATTAGTGTCG CCAATTCCTC CACCTCCACC ACCTTTGCCG 
CAGTCGCATC ACATCACAGG ACCTAAACCC TTACAGGACC TCGATTTCCA AAAGCATGCT 
ACGGAAATTC TTTGGAACAT GTTAAAGCAG GAGGAAGCAG ATCTACAGAA TCTGTGTGAT 
GTGTATAACA GAAGCCCTAA CCCCAGCCTG CAGGAGCAGA TAGAGAGGGC CAGGAAGAGG 
GTGCATCAGC TGCAGCATAA GATAAGGCCA GAAATACCCA GTACTGGGAA TTTGCTGCGT 
TCATACAGTG ACTGCAGCTC TGTGGTGTAT AAAGTCAAAG AAGGTCGCTT TTCTGTTGAT 
TCTCAGGATG GAGACAGTGG CCTGGATTCG GGAACTGAAC GGATATCATC GTTGAGTGAG 
TATTCCATGA ATCGGAATTC TGTCCTGTCA GACCTCGGAA TGGACAGCTC CCACACCTCC 
CCAATCATCC CAACGAAACT GTTTCACCAC CACCATCGGC GGCACGGGTC AGACACCACC 
TTTATTGCCA CGTCTGAGCA GGTATCAGAT CATAGGAGGT CTACGATAAT CGGGCCAGAA 
GAGGACTGTG ACCATGGATA TTACATGACA GAGCAGTCAG ATGGTCTCTT CCAAGATCTG 
GAAAAGTTAA AGACCCGGCC AGCCCATATG GGGGTGTTTC TACGTTATAT CTTCTCCCAG 
GCTGATCCAA ACCCACTGCT CTTTTACCTG AGTGCTGAGG CCTGTCAGCA TTTGAGTACA 
ACAGACGCCT TCACATTGAG GAAAGAAGTC TGGAACATCT TCCTTGACAA AAGTGCACCA 
CTGAGGGTTA AAATCCCTGA TCAGCTACTG AATGAAATTG AATCCCGTTT GCGGAACTCG 
GAAGATATCC GTGGTGTGTT TAGTGAAGCC CAGGAGGCGA TGCTGCCAGA GATACAGGAG 
CAGATACAGG ATTACAGGAC GAAAAGGACC ATGGGATTAG GGAGCCTGTA CGGGGAGAAT 
GACCTGTTCG ACCTTGAGAT TGACCCTCAC AGAGAACGTC ATGTTGCTGC TAAACAGATT 
GCACAGCTGG AGGACATACT ATCAAAGTAT GAGAAAGAGA TCAGCTCGCC CATGGCCTTT 
GCCTTGAGCA TGTATGTGAA GTACGCTGGG ATCCACACTC AAGATATCCG TCCCGCCAGC 
ATCTCCGAAA AAAGCATCCC CGACAGGGAA AAGTGGCTCC CGTTCTTCCC TAAGAACAAG 
AAAAGCAGCA ACGCCAAAAA GGATAAAGAG TCGGCTGAGG ACAAGAAGCG CAACCCCATC 
CTTAAGTATA TTAACAAGCC CAAGAGTACG TCTCAGAGTA CATTTCATGT TCCTTTGTCC 
CCTGGTGAAG TTAAACCCGG GAATGTGAGA AACATTATCC AGCACTTTGA AAACAACCAA 
CAATATGATG TTCCCGAACT TAACCTACAG CGGCTTTCAA CCGGGAGCTT CCCTGAAGAT 
CTACTGGGCT CTGACAGCAT CAGGTCGGAG GTGAAACTGG GCCGGTCAGA AAGCTTAAAG 
GGACGGGAAG AGTTGAAGAA ATCCCGCAAA TTGGACAATG TTCCTCGATC TCGCAGTGAC 
GTAGATATGG ACGCGGCAGC TGAGGCTACA CGGCTACACC AGTCGGCTTC ATCCTCTGCA 
TCCAGCCTAT CCGCAAGGTC ATTGGATAAT CCTACCCCAC CCTATACTCC CAAATTATGT 
CGAAGGAGCA TGGAGTCGCC CAATTTGGGG TTGACTGGAG AATTGCTGCT CTCCAACCTG 
CAGGAGGACG ACCTGGGTCA CCTCTCTGAC CTTGACCCTG AGCCCGACGC ACAGAACTGG 
CAGCATACGG TGGGTCGGGA CGTGGCGTTG CACCTGCCAC CCAAAGAGAG GGCACGGCAA 
GAGGTGATAC ATGAGCTGTT TGTGACAGAA GCCTCCCACC TTCGGGTCCT ACGAGTTCTG 
GATCTCGTTT TCTACCAGAA GATGCGCAAG GAGAACCTGC TGACCCCAGA TGAGCTCTGG 
CTTGTCTTCC CCAATCTCCC AGAACTGATT GAGATCCACA ACTCGCTGGC AGAGTCAATG 
AAGCGGGTGA GAGAAGAAGG GCCAATCATC AAAGAGATCG GTGACCTCAT GCTTTTGCAG 
TTTGATGGAG CTTCAGGGGA GGAAGTCCGA ATGGAAGCAG CCAAGTTCTG CTCGTACCAG 
ACATCTGCCC TTGAGTTGAT CAAAGCCAAA AAGAGGAAAG AGACCCGGTT CAGTCTCTTC 
ATGCAGGAAG CTGAAAGCAA CCCCCAGTGC CGACGACTGC AGCTGAAGGA CCTGATTGTG 
TCTGAGATGC AACGGCTGAC CAAGTACCCT CTGCTGCTGG AAAACGTCCT TAAGCACACA 
GACTCCGGGA CTCAGGAACA TGAAAAGCTG TACCGTTGCC GTGATCGGTG TCGAGATATT 
CTGAAATATG TCAACGAGTC CGTCAAACAG GCAGAAAATG AACATCGACT GGCTGAGTAT 
CAGAAGCGAC TGGACGCAAG CCACTTGGAG CGGTCGAATA ATCCTCTAGC TGGAGAGTTT 
AAGAACTTAG ACCTGACCAC AAGGCGAATG ATACATGAGG GGCCTCTCAC CTGGAGGGTA 
ACAAAAGACA AGAATATAGA CTTGCATGTT CTCTTGCTGG AAGATCTCCT CGTATTACTT 
CAGAAGCAGG ATGAGAAGCT GGTGCTCAAA TGCCATAGCA AGACCGCCAT GGTCTCCACC 
GACACCAAAC AAACCTTTAG TCCGGTCATT AAGCTGAATT CTGTCCTTGT TCGCTCGGTG 
GCTACAGATA AAAGAGCCCT GTTTATTATC TGCACTTCAG ACTTGGGACC TCAGATCTAC 
GAGCTGGTGG CACTGACATC CTCAGAGAAA AACACGTGGA AGGATTTATT GGAAGAAGCA 
ACCAATGGTG CCATGAGGAA TCCTCCCGGT GTCACCAAAC GGAAATCCCA GGACATCAAT 
AGACCAACCT CTGCCAGCAT GGTCCTACAG AGAGCCCACA TGCCCAAAGT CTTGGCAGAA 
GGAGTTCTAA GACATAACAG TGAGGACCCT CAATCAGGTG ATGAGAGCGC TGGGGAATTC 
TTAGGCCACC AAAAGCCAGA TGTCGTCCTA GAGGAATCGG AAAGGGAGGA AGATGAAGAA 
GATGAAGCTC GGACACCTAC CCTGCTGCCT ATCCCATCTA AAGAGGAGGA TGCCGGAGGT 
GGGACACGGG GGTTAGACTC TTCCAACCAT ATTCCCTACC AGTGGCCTGC AGTGACTGAA 
GGGCTGGCTG AGTCTGCACT GGATGATGTT CATAACCTCC GCCAGTTGAT CTTGAAGAAC 
ATGCTCCACC ACGGCCATTC CGAGTACAAC TCTTGCTCTG CCCCAGTGGC TGAGCCTGAA 
GATGACCTCA CGCCTACGCC TTCCATCATT GGAGGACACG GGCACCCATG GGACACTATG 
ACAGACGCAG TATTAGAAAA TCTAGGCGGA GATCAGACTA CTGAAGAGAA GGAGACCTCT 
GTTCCACCAT CAGTTAGCAG CTCTGAGAGC AGCAGGTCTG TTTTGAACTT TCCTCATGGA 
GATAGACCAG GTTCCAGAGA TGGGGAGGAG ACTGCTTGGA GTTTGGTTGA ACGCTACCCA 
CAAAGCAGTT CTGTTCATCA GAACCAATCA GAGCAACTAC ACTGTGAGGA CCATAGTAAC 
CAAAGCTACA GAGTTGTTAG AACAGCTGAG GTGGTGGGAA ATTATGATGT CATACCGTTG 
CCAGACAGCA GCCAATCACA GAGTGTGTTG CAGGAAGCTG GCGGTGGAGC TATTGTAGAT 
GGAAATTTCT TTTATGTAAC TGCGTCTACG GCTTCTGCAG CTTCTGCAAA GGAAGATAAT 
GAAGTCTCAG AATCCCAAAT GAACTCTGAC CGGGCGGGCT TGTCCTTCTT GGAATCTCAA 
GAGTCAAACC ACACGCCAAC TTACCAGCCA GAACTTGCCA CCCCACCATC AAGGAATACA 
GAAAGAGATC CACTTTCATC GGATCTCCTG CCCCCAAAAC TTGTCATTCA GGAAGTAGAT 
ATTATCTTCA GGACGATAGA GCAGCTGACG GCCAAACTCC ACAGGCTGAA GGTAGTAGAG 
TCAGCCCATC AGGAGCTTCT GCGATCCTTA GGAAGAAGTG CCACAGAAGA CCATTACCAT 
GAGAGAGAGC ACATGGCAGA GAAGGATAAT CAAGCTCAGA AGCATCAGGG GTCCAATTTG 
AACAGCTCCT CTCTATTCTT TGTGAAGAAC AGCATATCGG TCTCGCTTCA TCGAGATGGC 
AATACAGACC CACAGGAAGA TGCTATAAAT CTGGCCATGG GAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018088974.1
CDS	1131..5918
Translation	MSISSAQTSIDRLSSLSSASDGSAERRSPGHQRQPCNESNESTGLVQRCVIVQRDQNGFGFTVSGDRIVLVQSVRPGGAAMRAGVQEGDRIVKVNGTLVANSSHIEVVKLIKSGAYVALTLLGSPPPSMGMSGYQQDLSSSVSPRNILVSPIPPPPPPLPQSHHITGPKPLQDLDFQKHATEILWNMLKQEEADLQNLCDVYNRSPNPSLQEQIERARKRVHQLQHKIRPEIPSTGNLLRSYSDCSSVVYKVKEGRFSVDSQDGDSGLDSGTERISSLSEYSMNRNSVLSDLGMDSSHTSPIIPTKLFHHHHRRHGSDTTFIATSEQVSDHRRSTIIGPEEDCDHGYYMTEQSDGLFQDLEKLKTRPAHMGVFLRYIFSQADPNPLLFYLSAEACQHLSTTDAFTLRKEVWNIFLDKSAPLRVKIPDQLLNEIESRLRNSEDIRGVFSEAQEAMLPEIQEQIQDYRTKRTMGLGSLYGENDLFDLEIDPHRERHVAAKQIAQLEDILSKYEKEISSPMAFALSMYVKYAGIHTQDIRPASISEKSIPDREKWLPFFPKNKKSSNAKKDKESAEDKKRNPILKYINKPKSTSQSTFHVPLSPGEVKPGNVRNIIQHFENNQQYDVPELNLQRLSTGSFPEDLLGSDSIRSEVKLGRSESLKGREELKKSRKLDNVPRSRSDVDMDAAAEATRLHQSASSSASSLSARSLDNPTPPYTPKLCRRSMESPNLGLTGELLLSNLQEDDLGHLSDLDPEPDAQNWQHTVGRDVALHLPPKERARQEVIHELFVTEASHLRVLRVLDLVFYQKMRKENLLTPDELWLVFPNLPELIEIHNSLAESMKRVREEGPIIKEIGDLMLLQFDGASGEEVRMEAAKFCSYQTSALELIKAKKRKETRFSLFMQEAESNPQCRRLQLKDLIVSEMQRLTKYPLLLENVLKHTDSGTQEHEKLYRCRDRCRDILKYVNESVKQAENEHRLAEYQKRLDASHLERSNNPLAGEFKNLDLTTRRMIHEGPLTWRVTKDKNIDLHVLLLEDLLVLLQKQDEKLVLKCHSKTAMVSTDTKQTFSPVIKLNSVLVRSVATDKRALFIICTSDLGPQIYELVALTSSEKNTWKDLLEEATNGAMRNPPGVTKRKSQDINRPTSASMVLQRAHMPKVLAEGVLRHNSEDPQSGDESAGEFLGHQKPDVVLEESEREEDEEDEARTPTLLPIPSKEEDAGGGTRGLDSSNHIPYQWPAVTEGLAESALDDVHNLRQLILKNMLHHGHSEYNSCSAPVAEPEDDLTPTPSIIGGHGHPWDTMTDAVLENLGGDQTTEEKETSVPPSVSSSESSRSVLNFPHGDRPGSRDGEETAWSLVERYPQSSSVHQNQSEQLHCEDHSNQSYRVVRTAEVVGNYDVIPLPDSSQSQSVLQEAGGGAIVDGNFFYVTASTASAASAKEDNEVSESQMNSDRAGLSFLESQESNHTPTYQPELATPPSRNTERDPLSSDLLPPKLVIQEVDIIFRTIEQLTAKLHRLKVVESAHQELLRSLGRSATEDHYHEREHMAEKDNQAQKHQGSNLNSSSLFFVKNSISVSLHRDGNTDPQEDAINLAMGI

Target ORF information:

RefSeq Version	XM_018233485.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis rho guanine nucleotide exchange factor 11-like (LOC108700359), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018233485.1

ORF Insert Sequence:

ATGAGCATCA GTTCCGCACA GACTTCAATA GACCGGTTGA GTAGCCTGTC ATCGGCTAGT 
GACGGATCTG CCGAGCGAAG GTCTCCTGGA CACCAGCGTC AGCCTTGTAA CGAGAGTAAC 
GAGTCTACAG GCCTGGTCCA GCGCTGTGTC ATTGTTCAAA GGGACCAAAA TGGATTTGGT 
TTCACGGTCA GCGGAGATCG AATTGTTCTG GTTCAGTCTG TACGTCCAGG TGGGGCAGCA 
ATGCGAGCTG GGGTGCAGGA GGGAGACCGG ATTGTGAAGG TCAACGGCAC GCTGGTGGCT 
AACAGCTCAC ACATCGAAGT GGTCAAGCTG ATTAAATCCG GTGCTTACGT GGCTCTCACT 
CTCCTGGGTT CTCCACCTCC ATCCATGGGC ATGTCTGGGT ATCAGCAAGA CTTGAGCTCC 
TCTGTGTCCC CACGTAACAT ATTAGTGTCG CCAATTCCTC CACCTCCACC ACCTTTGCCG 
CAGTCGCATC ACATCACAGG ACCTAAACCC TTACAGGACC TCGATTTCCA AAAGCATGCT 
ACGGAAATTC TTTGGAACAT GTTAAAGCAG GAGGAAGCAG ATCTACAGAA TCTGTGTGAT 
GTGTATAACA GAAGCCCTAA CCCCAGCCTG CAGGAGCAGA TAGAGAGGGC CAGGAAGAGG 
GTGCATCAGC TGCAGCATAA GATAAGGCCA GAAATACCCA GTACTGGGAA TTTGCTGCGT 
TCATACAGTG ACTGCAGCTC TGTGGTGTAT AAAGTCAAAG AAGGTCGCTT TTCTGTTGAT 
TCTCAGGATG GAGACAGTGG CCTGGATTCG GGAACTGAAC GGATATCATC GTTGAGTGAG 
TATTCCATGA ATCGGAATTC TGTCCTGTCA GACCTCGGAA TGGACAGCTC CCACACCTCC 
CCAATCATCC CAACGAAACT GTTTCACCAC CACCATCGGC GGCACGGGTC AGACACCACC 
TTTATTGCCA CGTCTGAGCA GGTATCAGAT CATAGGAGGT CTACGATAAT CGGGCCAGAA 
GAGGACTGTG ACCATGGATA TTACATGACA GAGCAGTCAG ATGGTCTCTT CCAAGATCTG 
GAAAAGTTAA AGACCCGGCC AGCCCATATG GGGGTGTTTC TACGTTATAT CTTCTCCCAG 
GCTGATCCAA ACCCACTGCT CTTTTACCTG AGTGCTGAGG CCTGTCAGCA TTTGAGTACA 
ACAGACGCCT TCACATTGAG GAAAGAAGTC TGGAACATCT TCCTTGACAA AAGTGCACCA 
CTGAGGGTTA AAATCCCTGA TCAGCTACTG AATGAAATTG AATCCCGTTT GCGGAACTCG 
GAAGATATCC GTGGTGTGTT TAGTGAAGCC CAGGAGGCGA TGCTGCCAGA GATACAGGAG 
CAGATACAGG ATTACAGGAC GAAAAGGACC ATGGGATTAG GGAGCCTGTA CGGGGAGAAT 
GACCTGTTCG ACCTTGAGAT TGACCCTCAC AGAGAACGTC ATGTTGCTGC TAAACAGATT 
GCACAGCTGG AGGACATACT ATCAAAGTAT GAGAAAGAGA TCAGCTCGCC CATGGCCTTT 
GCCTTGAGCA TGTATGTGAA GTACGCTGGG ATCCACACTC AAGATATCCG TCCCGCCAGC 
ATCTCCGAAA AAAGCATCCC CGACAGGGAA AAGTGGCTCC CGTTCTTCCC TAAGAACAAG 
AAAAGCAGCA ACGCCAAAAA GGATAAAGAG TCGGCTGAGG ACAAGAAGCG CAACCCCATC 
CTTAAGTATA TTAACAAGCC CAAGAGTACG TCTCAGAGTA CATTTCATGT TCCTTTGTCC 
CCTGGTGAAG TTAAACCCGG GAATGTGAGA AACATTATCC AGCACTTTGA AAACAACCAA 
CAATATGATG TTCCCGAACT TAACCTACAG CGGCTTTCAA CCGGGAGCTT CCCTGAAGAT 
CTACTGGGCT CTGACAGCAT CAGGTCGGAG GTGAAACTGG GCCGGTCAGA AAGCTTAAAG 
GGACGGGAAG AGTTGAAGAA ATCCCGCAAA TTGGACAATG TTCCTCGATC TCGCAGTGAC 
GTAGATATGG ACGCGGCAGC TGAGGCTACA CGGCTACACC AGTCGGCTTC ATCCTCTGCA 
TCCAGCCTAT CCGCAAGGTC ATTGGATAAT CCTACCCCAC CCTATACTCC CAAATTATGT 
CGAAGGAGCA TGGAGTCGCC CAATTTGGGG TTGACTGGAG AATTGCTGCT CTCCAACCTG 
CAGGAGGACG ACCTGGGTCA CCTCTCTGAC CTTGACCCTG AGCCCGACGC ACAGAACTGG 
CAGCATACGG TGGGTCGGGA CGTGGCGTTG CACCTGCCAC CCAAAGAGAG GGCACGGCAA 
GAGGTGATAC ATGAGCTGTT TGTGACAGAA GCCTCCCACC TTCGGGTCCT ACGAGTTCTG 
GATCTCGTTT TCTACCAGAA GATGCGCAAG GAGAACCTGC TGACCCCAGA TGAGCTCTGG 
CTTGTCTTCC CCAATCTCCC AGAACTGATT GAGATCCACA ACTCGCTGGC AGAGTCAATG 
AAGCGGGTGA GAGAAGAAGG GCCAATCATC AAAGAGATCG GTGACCTCAT GCTTTTGCAG 
TTTGATGGAG CTTCAGGGGA GGAAGTCCGA ATGGAAGCAG CCAAGTTCTG CTCGTACCAG 
ACATCTGCCC TTGAGTTGAT CAAAGCCAAA AAGAGGAAAG AGACCCGGTT CAGTCTCTTC 
ATGCAGGAAG CTGAAAGCAA CCCCCAGTGC CGACGACTGC AGCTGAAGGA CCTGATTGTG 
TCTGAGATGC AACGGCTGAC CAAGTACCCT CTGCTGCTGG AAAACGTCCT TAAGCACACA 
GACTCCGGGA CTCAGGAACA TGAAAAGCTG TACCGTTGCC GTGATCGGTG TCGAGATATT 
CTGAAATATG TCAACGAGTC CGTCAAACAG GCAGAAAATG AACATCGACT GGCTGAGTAT 
CAGAAGCGAC TGGACGCAAG CCACTTGGAG CGGTCGAATA ATCCTCTAGC TGGAGAGTTT 
AAGAACTTAG ACCTGACCAC AAGGCGAATG ATACATGAGG GGCCTCTCAC CTGGAGGGTA 
ACAAAAGACA AGAATATAGA CTTGCATGTT CTCTTGCTGG AAGATCTCCT CGTATTACTT 
CAGAAGCAGG ATGAGAAGCT GGTGCTCAAA TGCCATAGCA AGACCGCCAT GGTCTCCACC 
GACACCAAAC AAACCTTTAG TCCGGTCATT AAGCTGAATT CTGTCCTTGT TCGCTCGGTG 
GCTACAGATA AAAGAGCCCT GTTTATTATC TGCACTTCAG ACTTGGGACC TCAGATCTAC 
GAGCTGGTGG CACTGACATC CTCAGAGAAA AACACGTGGA AGGATTTATT GGAAGAAGCA 
ACCAATGGTG CCATGAGGAA TCCTCCCGGT GTCACCAAAC GGAAATCCCA GGACATCAAT 
AGACCAACCT CTGCCAGCAT GGTCCTACAG AGAGCCCACA TGCCCAAAGT CTTGGCAGAA 
GGAGTTCTAA GACATAACAG TGAGGACCCT CAATCAGGTG ATGAGAGCGC TGGGGAATTC 
TTAGGCCACC AAAAGCCAGA TGTCGTCCTA GAGGAATCGG AAAGGGAGGA AGATGAAGAA 
GATGAAGCTC GGACACCTAC CCTGCTGCCT ATCCCATCTA AAGAGGAGGA TGCCGGAGGT 
GGGACACGGG GGTTAGACTC TTCCAACCAT ATTCCCTACC AGTGGCCTGC AGTGACTGAA 
GGGCTGGCTG AGTCTGCACT GGATGATGTT CATAACCTCC GCCAGTTGAT CTTGAAGAAC 
ATGCTCCACC ACGGCCATTC CGAGTACAAC TCTTGCTCTG CCCCAGTGGC TGAGCCTGAA 
GATGACCTCA CGCCTACGCC TTCCATCATT GGAGGACACG GGCACCCATG GGACACTATG 
ACAGACGCAG TATTAGAAAA TCTAGGCGGA GATCAGACTA CTGAAGAGAA GGAGACCTCT 
GTTCCACCAT CAGTTAGCAG CTCTGAGAGC AGCAGGTCTG TTTTGAACTT TCCTCATGGA 
GATAGACCAG GTTCCAGAGA TGGGGAGGAG ACTGCTTGGA GTTTGGTTGA ACGCTACCCA 
CAAAGCAGTT CTGTTCATCA GAACCAATCA GAGCAACTAC ACTGTGAGGA CCATAGTAAC 
CAAAGCTACA GAGTTGTTAG AACAGCTGAG GTGGTGGGAA ATTATGATGT CATACCGTTG 
CCAGACAGCA GCCAATCACA GAGTGTGTTG CAGGAAGCTG GCGGTGGAGC TATTGTAGAT 
GGAAATTTCT TTTATGTAAC TGCGTCTACG GCTTCTGCAG CTTCTGCAAA GGAAGATAAT 
GAAGTCTCAG AATCCCAAAT GAACTCTGAC CGGGCGGGCT TGTCCTTCTT GGAATCTCAA 
GAGTCAAACC ACACGCCAAC TTACCAGCCA GAACTTGCCA CCCCACCATC AAGGAATACA 
GAAAGAGATC CACTTTCATC GGATCTCCTG CCCCCAAAAC TTGTCATTCA GGAAGTAGAT 
ATTATCTTCA GGACGATAGA GCAGCTGACG GCCAAACTCC ACAGGCTGAA GGTAGTAGAG 
TCAGCCCATC AGGAGCTTCT GCGATCCTTA GGAAGAAGTG CCACAGAAGA CCATTACCAT 
GAGAGAGAGC ACATGGCAGA GAAGGATAAT CAAGCTCAGA AGCATCAGGG GTCCAATTTG 
AACAGCTCCT CTCTATTCTT TGTGAAGAAC AGCATATCGG TCTCGCTTCA TCGAGATGGC 
AATACAGACC CACAGGAAGA TGCTATAAAT CTGGCCATGG GAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108700359 ( XM_018233486.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018088975.1 Xenopus laevis rho guanine nucleotide exchange factor 11-like (LOC108700359), transcript variant X5, mRNA.

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CloneID	OAf44538
Clone ID Related Accession (Same CDS sequence)	XM_018233486.1
Accession Version	XM_018233486.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1474214400000
Organism	Xenopus laevis(African clawed frog)
Product	rho guanine nucleotide exchange factor 11-like isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030739.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATCA GTTCCGCACA GACTTCAATA GACCGATCCG CTTCTAAACG CTATCCTCGT 
TTGCCAAGGT TGAGTAGCCT GTCATCGGCT AGTGACGGAT CTGCCGAGCG AAGGTCTCCT 
GGACACCAGC GTCAGCCTTG TAACGAGAGT AACGAGTCTA CAGGCCTGGT CCAGCGCTGT 
GTCATTGTTC AAAGGGACCA AAATGGATTT GGTTTCACGG TCAGCGGAGA TCGAATTGTT 
CTGGTTCAGT CTGTACGTCC AGGTGGGGCA GCAATGCGAG CTGGGGTGCA GGAGGGAGAC 
CGGATTGTGA AGGTCAACGG CACGCTGGTG GCTAACAGCT CACACATCGA AGTGGTCAAG 
CTGATTAAAT CCGGTGCTTA CGTGGCTCTC ACTCTCCTGG GTTCTCCACC TCCATCCATG 
GGCATGTCTG GGTATCAGCA AGACTTGAGC TCCTCTGTGT CCCCACGTAA CATATTAGTG 
TCGCCAATTC CTCCACCTCC ACCACCTTTG CCGCAGTCGC ATCACATCAC AGGACCTAAA 
CCCTTACAGG ACCTCGATTT CCAAAAGCAT GCTACGGAAA TTCTTTGGAA CATGTTAAAG 
CAGGAGGAAG CAGATCTACA GAATCTGTGT GATGTGTATA ACAGAAGCCC TAACCCCAGC 
CTGCAGGAGC AGATAGAGAG GGCCAGGAAG AGGGTGCATC AGCTGCAGCA TAAGATAAGG 
CCAGAAATAC CCAGTACTGG GAATTTGCTG CGTTCATACA GTGACTGCAG CTCTGTGGTG 
TATAAAGTCA AAGAAGGTCG CTTTTCTGTT GATTCTCAGG ATGGAGACAG TGGCCTGGAT 
TCGGGAACTG AACGGATATC ATCGTTGAGT GAGTATTCCA TGAATCGGAA TTCTGTCCTG 
TCAGACCTCG GAATGGACAG CTCCCACACC TCCCCAATCA TCCCAACGAA ACTGTTTCAC 
CACCACCATC GGCGGCACGG GTCAGACACC ACCTTTATTG CCACGTCTGA GCAGGTATCA 
GATCATAGGA GGTCTACGAT AATCGGGCCA GAAGAGGACT GTGACCATGG ATATTACATG 
ACAGAGCAGT CAGATGGTCT CTTCCAAGAT CTGGAAAAGT TAAAGACCCG GCCAGCCCAT 
ATGGGGGTGT TTCTACGTTA TATCTTCTCC CAGGCTGATC CAAACCCACT GCTCTTTTAC 
CTGAGTGCTG AGGCCTGTCA GCATTTGAGT ACAACAGACG CCTTCACATT GAGGAAAGAA 
GTCTGGAACA TCTTCCTTGA CAAAAGTGCA CCACTGAGGG TTAAAATCCC TGATCAGCTA 
CTGAATGAAA TTGAATCCCG TTTGCGGAAC TCGGAAGATA TCCGTGGTGT GTTTAGTGAA 
GCCCAGGAGG CGATGCTGCC AGAGATACAG GAGCAGATAC AGGATTACAG GACGAAAAGG 
ACCATGGGAT TAGGGAGCCT GTACGGGGAG AATGACCTGT TCGACCTTGA GATTGACCCT 
CACAGAGAAC GTCATGTTGC TGCTAAACAG ATTGCACAGC TGGAGGACAT ACTATCAAAG 
TATGAGAAAG AGATCAGCTC GCCCATGGCC TTTGCCTTGA GCATGTATGT GAAGTACGCT 
GGGATCCACA CTCAAGATAT CCGTCCCGCC AGCATCTCCG AAAAAAGCAT CCCCGACAGG 
GAAAAGTGGC TCCCGTTCTT CCCTAAGAAC AAGAAAAGCA GCAACGCCAA AAAGGATAAA 
GAGTCGGCTG AGGACAAGAA GCGCAACCCC ATCCTTAAGT ATATTAACAA GCCCAAGAGT 
ACGTCTCAGA GTACATTTCA TGTTCCTTTG TCCCCTGGTG AAGTTAAACC CGGGAATGTG 
AGAAACATTA TCCAGCACTT TGAAAACAAC CAACAATATG ATGTTCCCGA ACTTAACCTA 
CAGCGGCTTT CAACCGGGAG CTTCCCTGAA GATCTACTGG GCTCTGACAG CATCAGGTCG 
GAGGTGAAAC TGGGCCGGTC AGAAAGCTTA AAGGGACGGG AAGAGTTGAA GAAATCCCGC 
AAATTGGACA ATGTTCCTCG ATCTCGCAGT GACGTAGATA TGGACGCGGC AGCTGAGGCT 
ACACGGCTAC ACCAGTCGGC TTCATCCTCT GCATCCAGCC TATCCGCAAG GTCATTGGAT 
AATCCTACCC CACCCTATAC TCCCAAATTA TGTCGAAGGA GCATGGAGTC GCCCAATTTG 
GGGTTGACTG GAGAATTGCT GCTCTCCAAC CTGCAGGAGG ACGACCTGGG TCACCTCTCT 
GACCTTGACC CTGAGCCCGA CGCACAGAAC TGGCAGCATA CGGTGGGTCG GGACGTGGCG 
TTGCACCTGC CACCCAAAGA GAGGGCACGG CAAGAGGTGA TACATGAGCT GTTTGTGACA 
GAAGCCTCCC ACCTTCGGGT CCTACGAGTT CTGGATCTCG TTTTCTACCA GAAGATGCGC 
AAGGAGAACC TGCTGACCCC AGATGAGCTC TGGCTTGTCT TCCCCAATCT CCCAGAACTG 
ATTGAGATCC ACAACTCGCT GGCAGAGTCA ATGAAGCGGG TGAGAGAAGA AGGGCCAATC 
ATCAAAGAGA TCGGTGACCT CATGCTTTTG CAGTTTGATG GAGCTTCAGG GGAGGAAGTC 
CGAATGGAAG CAGCCAAGTT CTGCTCGTAC CAGACATCTG CCCTTGAGTT GATCAAAGCC 
AAAAAGAGGA AAGAGACCCG GTTCAGTCTC TTCATGCAGG AAGCTGAAAG CAACCCCCAG 
TGCCGACGAC TGCAGCTGAA GGACCTGATT GTGTCTGAGA TGCAACGGCT GACCAAGTAC 
CCTCTGCTGC TGGAAAACGT CCTTAAGCAC ACAGACTCCG GGACTCAGGA ACATGAAAAG 
CTGTACCGTT GCCGTGATCG GTGTCGAGAT ATTCTGAAAT ATGTCAACGA GTCCGTCAAA 
CAGGCAGAAA ATGAACATCG ACTGGCTGAG TATCAGAAGC GACTGGACGC AAGCCACTTG 
GAGCGGTCGA ATAATCCTCT AGCTGGAGAG TTTAAGAACT TAGACCTGAC CACAAGGCGA 
ATGATACATG AGGGGCCTCT CACCTGGAGG GTAACAAAAG ACAAGAATAT AGACTTGCAT 
GTTCTCTTGC TGGAAGATCT CCTCGTATTA CTTCAGAAGC AGGATGAGAA GCTGGTGCTC 
AAATGCCATA GCAAGACCGC CATGGTCTCC ACCGACACCA AACAAACCTT TAGTCCGGTC 
ATTAAGCTGA ATTCTGTCCT TGTTCGCTCG GTGGCTACAG ATAAAAGAGC CCTGTTTATT 
ATCTGCACTT CAGACTTGGG ACCTCAGATC TACGAGCTGG TGGCACTGAC ATCCTCAGAG 
AAAAACACGT GGAAGGATTT ATTGGAAGAA GCAACCAATG GTGCCATGAG GAATCCTCCC 
GGTGTCACCA AACGGAAATC CCAGGACATC AATAGACCAA CCTCTGCCAG CATGGTCCTA 
CAGAGAGCCC ACATGCCCAA AGTCTTGGCA GAAGGAGTTC TAAGACATAA CAGTGAGGAC 
CCTCAATCAG GTGATGAGAG CGCTGGGGAA TTCTTAGGCC ACCAAAAGCC AGATGTCGTC 
CTAGAGGAAT CGGAAAGGGA GGAAGATGAA GAAGATGAAG CTCGGACACC TACCCTGCTG 
CCTATCCCAT CTAAAGAGGA GGATGCCGGA GGTGGGACAC GGGGGTTAGA CTCTTCCAAC 
CATATTCCCT ACCAGTGGCC TGCAGTGACT GAAGGGCTGG CTGAGTCTGC ACTGGATGAT 
GTTCATAACC TCCGCCAGTT GATCTTGAAG AACATGCTCC ACCACGGCCA TTCCGAGTAC 
AACTCTTGCT CTGCCCCAGT GGCTGAGCCT GAAGATGACC TCACGCCTAC GCCTTCCATC 
ATTGGAGGAC ACGGGCACCC ATGGGACACT ATGACAGACG CAGTATTAGA AAATCTAGGC 
GGAGATCAGA CTACTGAAGA GAAGGAGACC TCTGTTCCAC CATCAGTTAG CAGCTCTGAG 
AGCAGCAGGT CTGTTTTGAA CTTTCCTCAT GGAGATAGAC CAGGTTCCAG AGATGGGGAG 
GAGACTGCTT GGAGTTTGGT TGAACGCTAC CCACAAAGCA GTTCTGTTCA TCAGAACCAA 
TCAGAGCAAC TACACTGTGA GGACCATAGT AACCAAAGCT ACAGAGTTGT TAGAACAGGA 
AATTTCTTTT ATGTAACTGC GTCTACGGCT TCTGCAGCTT CTGCAAAGGA AGATAATGAA 
GTCTCAGAAT CCCAAATGAA CTCTGACCGG GCGGGCTTGT CCTTCTTGGA ATCTCAAGAG 
TCAAACCACA CGCCAACTTA CCAGCCAGAA CTTGCCACCC CACCATCAAG GAATACAGAA 
AGAGATCCAC TTTCATCGGA TCTCCTGCCC CCAAAACTTG TCATTCAGGA AGTAGATATT 
ATCTTCAGGA CGATAGAGCA GCTGACGGCC AAACTCCACA GGCTGAAGGT AGTAGAGTCA 
GCCCATCAGG AGCTTCTGCG ATCCTTAGGA AGAAGTGCCA CAGAAGACCA TTACCATGAG 
AGAGAGCACA TGGCAGAGAA GGATAATCAA GCTCAGAAGC ATCAGGGGTC CAATTTGAAC 
AGCTCCTCTC TATTCTTTGT GAAGAACAGC ATATCGGTCT CGCTTCATCG AGATGGCAAT 
ACAGACCCAC AGGAAGATGC TATAAATCTG GCCATGGGAA TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018088975.1
CDS	1130..5854
Translation	MSISSAQTSIDRSASKRYPRLPRLSSLSSASDGSAERRSPGHQRQPCNESNESTGLVQRCVIVQRDQNGFGFTVSGDRIVLVQSVRPGGAAMRAGVQEGDRIVKVNGTLVANSSHIEVVKLIKSGAYVALTLLGSPPPSMGMSGYQQDLSSSVSPRNILVSPIPPPPPPLPQSHHITGPKPLQDLDFQKHATEILWNMLKQEEADLQNLCDVYNRSPNPSLQEQIERARKRVHQLQHKIRPEIPSTGNLLRSYSDCSSVVYKVKEGRFSVDSQDGDSGLDSGTERISSLSEYSMNRNSVLSDLGMDSSHTSPIIPTKLFHHHHRRHGSDTTFIATSEQVSDHRRSTIIGPEEDCDHGYYMTEQSDGLFQDLEKLKTRPAHMGVFLRYIFSQADPNPLLFYLSAEACQHLSTTDAFTLRKEVWNIFLDKSAPLRVKIPDQLLNEIESRLRNSEDIRGVFSEAQEAMLPEIQEQIQDYRTKRTMGLGSLYGENDLFDLEIDPHRERHVAAKQIAQLEDILSKYEKEISSPMAFALSMYVKYAGIHTQDIRPASISEKSIPDREKWLPFFPKNKKSSNAKKDKESAEDKKRNPILKYINKPKSTSQSTFHVPLSPGEVKPGNVRNIIQHFENNQQYDVPELNLQRLSTGSFPEDLLGSDSIRSEVKLGRSESLKGREELKKSRKLDNVPRSRSDVDMDAAAEATRLHQSASSSASSLSARSLDNPTPPYTPKLCRRSMESPNLGLTGELLLSNLQEDDLGHLSDLDPEPDAQNWQHTVGRDVALHLPPKERARQEVIHELFVTEASHLRVLRVLDLVFYQKMRKENLLTPDELWLVFPNLPELIEIHNSLAESMKRVREEGPIIKEIGDLMLLQFDGASGEEVRMEAAKFCSYQTSALELIKAKKRKETRFSLFMQEAESNPQCRRLQLKDLIVSEMQRLTKYPLLLENVLKHTDSGTQEHEKLYRCRDRCRDILKYVNESVKQAENEHRLAEYQKRLDASHLERSNNPLAGEFKNLDLTTRRMIHEGPLTWRVTKDKNIDLHVLLLEDLLVLLQKQDEKLVLKCHSKTAMVSTDTKQTFSPVIKLNSVLVRSVATDKRALFIICTSDLGPQIYELVALTSSEKNTWKDLLEEATNGAMRNPPGVTKRKSQDINRPTSASMVLQRAHMPKVLAEGVLRHNSEDPQSGDESAGEFLGHQKPDVVLEESEREEDEEDEARTPTLLPIPSKEEDAGGGTRGLDSSNHIPYQWPAVTEGLAESALDDVHNLRQLILKNMLHHGHSEYNSCSAPVAEPEDDLTPTPSIIGGHGHPWDTMTDAVLENLGGDQTTEEKETSVPPSVSSSESSRSVLNFPHGDRPGSRDGEETAWSLVERYPQSSSVHQNQSEQLHCEDHSNQSYRVVRTGNFFYVTASTASAASAKEDNEVSESQMNSDRAGLSFLESQESNHTPTYQPELATPPSRNTERDPLSSDLLPPKLVIQEVDIIFRTIEQLTAKLHRLKVVESAHQELLRSLGRSATEDHYHEREHMAEKDNQAQKHQGSNLNSSSLFFVKNSISVSLHRDGNTDPQEDAINLAMGI

Target ORF information:

RefSeq Version	XM_018233486.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis rho guanine nucleotide exchange factor 11-like (LOC108700359), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018233486.1

ORF Insert Sequence:

ATGAGCATCA GTTCCGCACA GACTTCAATA GACCGATCCG CTTCTAAACG CTATCCTCGT 
TTGCCAAGGT TGAGTAGCCT GTCATCGGCT AGTGACGGAT CTGCCGAGCG AAGGTCTCCT 
GGACACCAGC GTCAGCCTTG TAACGAGAGT AACGAGTCTA CAGGCCTGGT CCAGCGCTGT 
GTCATTGTTC AAAGGGACCA AAATGGATTT GGTTTCACGG TCAGCGGAGA TCGAATTGTT 
CTGGTTCAGT CTGTACGTCC AGGTGGGGCA GCAATGCGAG CTGGGGTGCA GGAGGGAGAC 
CGGATTGTGA AGGTCAACGG CACGCTGGTG GCTAACAGCT CACACATCGA AGTGGTCAAG 
CTGATTAAAT CCGGTGCTTA CGTGGCTCTC ACTCTCCTGG GTTCTCCACC TCCATCCATG 
GGCATGTCTG GGTATCAGCA AGACTTGAGC TCCTCTGTGT CCCCACGTAA CATATTAGTG 
TCGCCAATTC CTCCACCTCC ACCACCTTTG CCGCAGTCGC ATCACATCAC AGGACCTAAA 
CCCTTACAGG ACCTCGATTT CCAAAAGCAT GCTACGGAAA TTCTTTGGAA CATGTTAAAG 
CAGGAGGAAG CAGATCTACA GAATCTGTGT GATGTGTATA ACAGAAGCCC TAACCCCAGC 
CTGCAGGAGC AGATAGAGAG GGCCAGGAAG AGGGTGCATC AGCTGCAGCA TAAGATAAGG 
CCAGAAATAC CCAGTACTGG GAATTTGCTG CGTTCATACA GTGACTGCAG CTCTGTGGTG 
TATAAAGTCA AAGAAGGTCG CTTTTCTGTT GATTCTCAGG ATGGAGACAG TGGCCTGGAT 
TCGGGAACTG AACGGATATC ATCGTTGAGT GAGTATTCCA TGAATCGGAA TTCTGTCCTG 
TCAGACCTCG GAATGGACAG CTCCCACACC TCCCCAATCA TCCCAACGAA ACTGTTTCAC 
CACCACCATC GGCGGCACGG GTCAGACACC ACCTTTATTG CCACGTCTGA GCAGGTATCA 
GATCATAGGA GGTCTACGAT AATCGGGCCA GAAGAGGACT GTGACCATGG ATATTACATG 
ACAGAGCAGT CAGATGGTCT CTTCCAAGAT CTGGAAAAGT TAAAGACCCG GCCAGCCCAT 
ATGGGGGTGT TTCTACGTTA TATCTTCTCC CAGGCTGATC CAAACCCACT GCTCTTTTAC 
CTGAGTGCTG AGGCCTGTCA GCATTTGAGT ACAACAGACG CCTTCACATT GAGGAAAGAA 
GTCTGGAACA TCTTCCTTGA CAAAAGTGCA CCACTGAGGG TTAAAATCCC TGATCAGCTA 
CTGAATGAAA TTGAATCCCG TTTGCGGAAC TCGGAAGATA TCCGTGGTGT GTTTAGTGAA 
GCCCAGGAGG CGATGCTGCC AGAGATACAG GAGCAGATAC AGGATTACAG GACGAAAAGG 
ACCATGGGAT TAGGGAGCCT GTACGGGGAG AATGACCTGT TCGACCTTGA GATTGACCCT 
CACAGAGAAC GTCATGTTGC TGCTAAACAG ATTGCACAGC TGGAGGACAT ACTATCAAAG 
TATGAGAAAG AGATCAGCTC GCCCATGGCC TTTGCCTTGA GCATGTATGT GAAGTACGCT 
GGGATCCACA CTCAAGATAT CCGTCCCGCC AGCATCTCCG AAAAAAGCAT CCCCGACAGG 
GAAAAGTGGC TCCCGTTCTT CCCTAAGAAC AAGAAAAGCA GCAACGCCAA AAAGGATAAA 
GAGTCGGCTG AGGACAAGAA GCGCAACCCC ATCCTTAAGT ATATTAACAA GCCCAAGAGT 
ACGTCTCAGA GTACATTTCA TGTTCCTTTG TCCCCTGGTG AAGTTAAACC CGGGAATGTG 
AGAAACATTA TCCAGCACTT TGAAAACAAC CAACAATATG ATGTTCCCGA ACTTAACCTA 
CAGCGGCTTT CAACCGGGAG CTTCCCTGAA GATCTACTGG GCTCTGACAG CATCAGGTCG 
GAGGTGAAAC TGGGCCGGTC AGAAAGCTTA AAGGGACGGG AAGAGTTGAA GAAATCCCGC 
AAATTGGACA ATGTTCCTCG ATCTCGCAGT GACGTAGATA TGGACGCGGC AGCTGAGGCT 
ACACGGCTAC ACCAGTCGGC TTCATCCTCT GCATCCAGCC TATCCGCAAG GTCATTGGAT 
AATCCTACCC CACCCTATAC TCCCAAATTA TGTCGAAGGA GCATGGAGTC GCCCAATTTG 
GGGTTGACTG GAGAATTGCT GCTCTCCAAC CTGCAGGAGG ACGACCTGGG TCACCTCTCT 
GACCTTGACC CTGAGCCCGA CGCACAGAAC TGGCAGCATA CGGTGGGTCG GGACGTGGCG 
TTGCACCTGC CACCCAAAGA GAGGGCACGG CAAGAGGTGA TACATGAGCT GTTTGTGACA 
GAAGCCTCCC ACCTTCGGGT CCTACGAGTT CTGGATCTCG TTTTCTACCA GAAGATGCGC 
AAGGAGAACC TGCTGACCCC AGATGAGCTC TGGCTTGTCT TCCCCAATCT CCCAGAACTG 
ATTGAGATCC ACAACTCGCT GGCAGAGTCA ATGAAGCGGG TGAGAGAAGA AGGGCCAATC 
ATCAAAGAGA TCGGTGACCT CATGCTTTTG CAGTTTGATG GAGCTTCAGG GGAGGAAGTC 
CGAATGGAAG CAGCCAAGTT CTGCTCGTAC CAGACATCTG CCCTTGAGTT GATCAAAGCC 
AAAAAGAGGA AAGAGACCCG GTTCAGTCTC TTCATGCAGG AAGCTGAAAG CAACCCCCAG 
TGCCGACGAC TGCAGCTGAA GGACCTGATT GTGTCTGAGA TGCAACGGCT GACCAAGTAC 
CCTCTGCTGC TGGAAAACGT CCTTAAGCAC ACAGACTCCG GGACTCAGGA ACATGAAAAG 
CTGTACCGTT GCCGTGATCG GTGTCGAGAT ATTCTGAAAT ATGTCAACGA GTCCGTCAAA 
CAGGCAGAAA ATGAACATCG ACTGGCTGAG TATCAGAAGC GACTGGACGC AAGCCACTTG 
GAGCGGTCGA ATAATCCTCT AGCTGGAGAG TTTAAGAACT TAGACCTGAC CACAAGGCGA 
ATGATACATG AGGGGCCTCT CACCTGGAGG GTAACAAAAG ACAAGAATAT AGACTTGCAT 
GTTCTCTTGC TGGAAGATCT CCTCGTATTA CTTCAGAAGC AGGATGAGAA GCTGGTGCTC 
AAATGCCATA GCAAGACCGC CATGGTCTCC ACCGACACCA AACAAACCTT TAGTCCGGTC 
ATTAAGCTGA ATTCTGTCCT TGTTCGCTCG GTGGCTACAG ATAAAAGAGC CCTGTTTATT 
ATCTGCACTT CAGACTTGGG ACCTCAGATC TACGAGCTGG TGGCACTGAC ATCCTCAGAG 
AAAAACACGT GGAAGGATTT ATTGGAAGAA GCAACCAATG GTGCCATGAG GAATCCTCCC 
GGTGTCACCA AACGGAAATC CCAGGACATC AATAGACCAA CCTCTGCCAG CATGGTCCTA 
CAGAGAGCCC ACATGCCCAA AGTCTTGGCA GAAGGAGTTC TAAGACATAA CAGTGAGGAC 
CCTCAATCAG GTGATGAGAG CGCTGGGGAA TTCTTAGGCC ACCAAAAGCC AGATGTCGTC 
CTAGAGGAAT CGGAAAGGGA GGAAGATGAA GAAGATGAAG CTCGGACACC TACCCTGCTG 
CCTATCCCAT CTAAAGAGGA GGATGCCGGA GGTGGGACAC GGGGGTTAGA CTCTTCCAAC 
CATATTCCCT ACCAGTGGCC TGCAGTGACT GAAGGGCTGG CTGAGTCTGC ACTGGATGAT 
GTTCATAACC TCCGCCAGTT GATCTTGAAG AACATGCTCC ACCACGGCCA TTCCGAGTAC 
AACTCTTGCT CTGCCCCAGT GGCTGAGCCT GAAGATGACC TCACGCCTAC GCCTTCCATC 
ATTGGAGGAC ACGGGCACCC ATGGGACACT ATGACAGACG CAGTATTAGA AAATCTAGGC 
GGAGATCAGA CTACTGAAGA GAAGGAGACC TCTGTTCCAC CATCAGTTAG CAGCTCTGAG 
AGCAGCAGGT CTGTTTTGAA CTTTCCTCAT GGAGATAGAC CAGGTTCCAG AGATGGGGAG 
GAGACTGCTT GGAGTTTGGT TGAACGCTAC CCACAAAGCA GTTCTGTTCA TCAGAACCAA 
TCAGAGCAAC TACACTGTGA GGACCATAGT AACCAAAGCT ACAGAGTTGT TAGAACAGGA 
AATTTCTTTT ATGTAACTGC GTCTACGGCT TCTGCAGCTT CTGCAAAGGA AGATAATGAA 
GTCTCAGAAT CCCAAATGAA CTCTGACCGG GCGGGCTTGT CCTTCTTGGA ATCTCAAGAG 
TCAAACCACA CGCCAACTTA CCAGCCAGAA CTTGCCACCC CACCATCAAG GAATACAGAA 
AGAGATCCAC TTTCATCGGA TCTCCTGCCC CCAAAACTTG TCATTCAGGA AGTAGATATT 
ATCTTCAGGA CGATAGAGCA GCTGACGGCC AAACTCCACA GGCTGAAGGT AGTAGAGTCA 
GCCCATCAGG AGCTTCTGCG ATCCTTAGGA AGAAGTGCCA CAGAAGACCA TTACCATGAG 
AGAGAGCACA TGGCAGAGAA GGATAATCAA GCTCAGAAGC ATCAGGGGTC CAATTTGAAC 
AGCTCCTCTC TATTCTTTGT GAAGAACAGC ATATCGGTCT CGCTTCATCG AGATGGCAAT 
ACAGACCCAC AGGAAGATGC TATAAATCTG GCCATGGGAA TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108700359 ( XM_018233487.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018088976.1 Xenopus laevis rho guanine nucleotide exchange factor 11-like (LOC108700359), transcript variant X6, mRNA.

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CloneID	OAf44539
Clone ID Related Accession (Same CDS sequence)	XM_018233487.1
Accession Version	XM_018233487.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4689bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1474214400000
Organism	Xenopus laevis(African clawed frog)
Product	rho guanine nucleotide exchange factor 11-like isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030739.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATCA GTTCCGCACA GACTTCAATA GACCGATCCG CTTCTAAACG CTATCCTCGT 
TTGCCAAGGT TGAGTAGCCT GTCATCGGCT AGTGACGGAT CTGCCGAGCG AAGGTCTCCT 
GGACACCAGC GTCAGCCTTG TAACGAGAGT AACGAGTCTA CAGGCCTGGT CCAGCGCTGT 
GTCATTGTTC AAAGGGACCA AAATGGATTT GGTTTCACGG TCAGCGGAGA TCGAATTGTT 
CTGGTTCAGT CTGTACGTCC AGGTGGGGCA GCAATGCGAG CTGGGGTGCA GGAGGGAGAC 
CGGATTGTGA AGGTCAACGG CACGCTGGTG GCTAACAGCT CACACATCGA AGTGGTCAAG 
CTGATTAAAT CCGGTGCTTA CGTGGCTCTC ACTCTCCTGG GTTCTCCACC TCCATCCATG 
GGCATGTCTG GGTATCAGCA AGACTTGAGC TCCTCTGTGT CCCCACGTAA CATATTAGTG 
TCGCCAATTC CTCCACCTCC ACCACCTTTG CCGCAGTCGC ATCACATCAC AGGACCTAAA 
CCCTTACAGG ACCTCGATTT CCAAAAGCAT GCTACGGAAA TTCTTTGGAA CATGTTAAAG 
CAGGAGGAAG CAGATCTACA GAATCTGTGT GATGTGTATA ACAGAAGCCC TAACCCCAGC 
CTGCAGGAGC AGATAGAGAG GGCCAGGAAG AGGGTGCATC AGCTGCAGCA TAAGATAAGG 
CCAGAAATAC CCAGTACTGG GTATTCCATG AATCGGAATT CTGTCCTGTC AGACCTCGGA 
ATGGACAGCT CCCACACCTC CCCAATCATC CCAACGAAAC TGTTTCACCA CCACCATCGG 
CGGCACGGGT CAGACACCAC CTTTATTGCC ACGTCTGAGC AGGTATCAGA TCATAGGAGG 
TCTACGATAA TCGGGCCAGA AGAGGACTGT GACCATGGAT ATTACATGAC AGAGCAGTCA 
GATGGTCTCT TCCAAGATCT GGAAAAGTTA AAGACCCGGC CAGCCCATAT GGGGGTGTTT 
CTACGTTATA TCTTCTCCCA GGCTGATCCA AACCCACTGC TCTTTTACCT GAGTGCTGAG 
GCCTGTCAGC ATTTGAGTAC AACAGACGCC TTCACATTGA GGAAAGAAGT CTGGAACATC 
TTCCTTGACA AAAGTGCACC ACTGAGGGTT AAAATCCCTG ATCAGCTACT GAATGAAATT 
GAATCCCGTT TGCGGAACTC GGAAGATATC CGTGGTGTGT TTAGTGAAGC CCAGGAGGCG 
ATGCTGCCAG AGATACAGGA GCAGATACAG GATTACAGGA CGAAAAGGAC CATGGGATTA 
GGGAGCCTGT ACGGGGAGAA TGACCTGTTC GACCTTGAGA TTGACCCTCA CAGAGAACGT 
CATGTTGCTG CTAAACAGAT TGCACAGCTG GAGGACATAC TATCAAAGTA TGAGAAAGAG 
ATCAGCTCGC CCATGGCCTT TGCCTTGAGC ATGTATGTGA AGTACGCTGG GATCCACACT 
CAAGATATCC GTCCCGCCAG CATCTCCGAA AAAAGCATCC CCGACAGGGA AAAGTGGCTC 
CCGTTCTTCC CTAAGAACAA GAAAAGCAGC AACGCCAAAA AGGATAAAGA GTCGGCTGAG 
GACAAGAAGC GCAACCCCAT CCTTAAGTAT ATTAACAAGC CCAAGAGTAC GTCTCAGAGT 
ACATTTCATG TTCCTTTGTC CCCTGGTGAA GTTAAACCCG GGAATGTGAG AAACATTATC 
CAGCACTTTG AAAACAACCA ACAATATGAT GTTCCCGAAC TTAACCTACA GCGGCTTTCA 
ACCGGGAGCT TCCCTGAAGA TCTACTGGGC TCTGACAGCA TCAGGTCGGA GGTGAAACTG 
GGCCGGTCAG AAAGCTTAAA GGGACGGGAA GAGTTGAAGA AATCCCGCAA ATTGGACAAT 
GTTCCTCGAT CTCGCAGTGA CGTAGATATG GACGCGGCAG CTGAGGCTAC ACGGCTACAC 
CAGTCGGCTT CATCCTCTGC ATCCAGCCTA TCCGCAAGGT CATTGGATAA TCCTACCCCA 
CCCTATACTC CCAAATTATG TCGAAGGAGC ATGGAGTCGC CCAATTTGGG GTTGACTGGA 
GAATTGCTGC TCTCCAACCT GCAGGAGGAC GACCTGGGTC ACCTCTCTGA CCTTGACCCT 
GAGCCCGACG CACAGAACTG GCAGCATACG GTGGGTCGGG ACGTGGCGTT GCACCTGCCA 
CCCAAAGAGA GGGCACGGCA AGAGGTGATA CATGAGCTGT TTGTGACAGA AGCCTCCCAC 
CTTCGGGTCC TACGAGTTCT GGATCTCGTT TTCTACCAGA AGATGCGCAA GGAGAACCTG 
CTGACCCCAG ATGAGCTCTG GCTTGTCTTC CCCAATCTCC CAGAACTGAT TGAGATCCAC 
AACTCGCTGG CAGAGTCAAT GAAGCGGGTG AGAGAAGAAG GGCCAATCAT CAAAGAGATC 
GGTGACCTCA TGCTTTTGCA GTTTGATGGA GCTTCAGGGG AGGAAGTCCG AATGGAAGCA 
GCCAAGTTCT GCTCGTACCA GACATCTGCC CTTGAGTTGA TCAAAGCCAA AAAGAGGAAA 
GAGACCCGGT TCAGTCTCTT CATGCAGGAA GCTGAAAGCA ACCCCCAGTG CCGACGACTG 
CAGCTGAAGG ACCTGATTGT GTCTGAGATG CAACGGCTGA CCAAGTACCC TCTGCTGCTG 
GAAAACGTCC TTAAGCACAC AGACTCCGGG ACTCAGGAAC ATGAAAAGCT GTACCGTTGC 
CGTGATCGGT GTCGAGATAT TCTGAAATAT GTCAACGAGT CCGTCAAACA GGCAGAAAAT 
GAACATCGAC TGGCTGAGTA TCAGAAGCGA CTGGACGCAA GCCACTTGGA GCGGTCGAAT 
AATCCTCTAG CTGGAGAGTT TAAGAACTTA GACCTGACCA CAAGGCGAAT GATACATGAG 
GGGCCTCTCA CCTGGAGGGT AACAAAAGAC AAGAATATAG ACTTGCATGT TCTCTTGCTG 
GAAGATCTCC TCGTATTACT TCAGAAGCAG GATGAGAAGC TGGTGCTCAA ATGCCATAGC 
AAGACCGCCA TGGTCTCCAC CGACACCAAA CAAACCTTTA GTCCGGTCAT TAAGCTGAAT 
TCTGTCCTTG TTCGCTCGGT GGCTACAGAT AAAAGAGCCC TGTTTATTAT CTGCACTTCA 
GACTTGGGAC CTCAGATCTA CGAGCTGGTG GCACTGACAT CCTCAGAGAA AAACACGTGG 
AAGGATTTAT TGGAAGAAGC AACCAATGGT GCCATGAGGA ATCCTCCCGG TGTCACCAAA 
CGGAAATCCC AGGACATCAA TAGACCAACC TCTGCCAGCA TGGTCCTACA GAGAGCCCAC 
ATGCCCAAAG TCTTGGCAGA AGGAGTTCTA AGACATAACA GTGAGGACCC TCAATCAGGT 
GATGAGAGCG CTGGGGAATT CTTAGGCCAC CAAAAGCCAG ATGTCGTCCT AGAGGAATCG 
GAAAGGGAGG AAGATGAAGA AGATGAAGCT CGGACACCTA CCCTGCTGCC TATCCCATCT 
AAAGAGGAGG ATGCCGGAGG TGGGACACGG GGGTTAGACT CTTCCAACCA TATTCCCTAC 
CAGTGGCCTG CAGTGACTGA AGGGCTGGCT GAGTCTGCAC TGGATGATGT TCATAACCTC 
CGCCAGTTGA TCTTGAAGAA CATGCTCCAC CACGGCCATT CCGAGTACAA CTCTTGCTCT 
GCCCCAGTGG CTGAGCCTGA AGATGACCTC ACGCCTACGC CTTCCATCAT TGGAGGACAC 
GGGCACCCAT GGGACACTAT GACAGACGCA GTATTAGAAA ATCTAGGCGG AGATCAGACT 
ACTGAAGAGA AGGAGACCTC TGTTCCACCA TCAGTTAGCA GCTCTGAGAG CAGCAGGTCT 
GTTTTGAACT TTCCTCATGG AGATAGACCA GGTTCCAGAG ATGGGGAGGA GACTGCTTGG 
AGTTTGGTTG AACGCTACCC ACAAAGCAGT TCTGTTCATC AGAACCAATC AGAGCAACTA 
CACTGTGAGG ACCATAGTAA CCAAAGCTAC AGAGTTGTTA GAACAGCTGA GGTGGTGGGA 
AATTATGATG TCATACCGTT GCCAGACAGC AGCCAATCAC AGAGTGTGTT GCAGGAAGCT 
GGCGGTGGAG CTATTGTAGA TGGAAATTTC TTTTATGTAA CTGCGTCTAC GGCTTCTGCA 
GCTTCTGCAA AGGAAGATAA TGAAGTCTCA GAATCCCAAA TGAACTCTGA CCGGGCGGGC 
TTGTCCTTCT TGGAATCTCA AGAGTCAAAC CACACGCCAA CTTACCAGCC AGAACTTGCC 
ACCCCACCAT CAAGGAATAC AGAAAGAGAT CCACTTTCAT CGGATCTCCT GCCCCCAAAA 
CTTGTCATTC AGGAAGTAGA TATTATCTTC AGGACGATAG AGCAGCTGAC GGCCAAACTC 
CACAGGCTGA AGGTAGTAGA GTCAGCCCAT CAGGAGCTTC TGCGATCCTT AGGAAGAAGT 
GCCACAGAAG ACCATTACCA TGAGAGAGAG CACATGGCAG AGAAGGATAA TCAAGCTCAG 
AAGCATCAGG GGTCCAATTT GAACAGCTCC TCTCTATTCT TTGTGAAGAA CAGCATATCG 
GTCTCGCTTC ATCGAGATGG CAATACAGAC CCACAGGAAG ATGCTATAAA TCTGGCCATG 
GGAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018088976.1
CDS	1131..5819
Translation	MSISSAQTSIDRSASKRYPRLPRLSSLSSASDGSAERRSPGHQRQPCNESNESTGLVQRCVIVQRDQNGFGFTVSGDRIVLVQSVRPGGAAMRAGVQEGDRIVKVNGTLVANSSHIEVVKLIKSGAYVALTLLGSPPPSMGMSGYQQDLSSSVSPRNILVSPIPPPPPPLPQSHHITGPKPLQDLDFQKHATEILWNMLKQEEADLQNLCDVYNRSPNPSLQEQIERARKRVHQLQHKIRPEIPSTGYSMNRNSVLSDLGMDSSHTSPIIPTKLFHHHHRRHGSDTTFIATSEQVSDHRRSTIIGPEEDCDHGYYMTEQSDGLFQDLEKLKTRPAHMGVFLRYIFSQADPNPLLFYLSAEACQHLSTTDAFTLRKEVWNIFLDKSAPLRVKIPDQLLNEIESRLRNSEDIRGVFSEAQEAMLPEIQEQIQDYRTKRTMGLGSLYGENDLFDLEIDPHRERHVAAKQIAQLEDILSKYEKEISSPMAFALSMYVKYAGIHTQDIRPASISEKSIPDREKWLPFFPKNKKSSNAKKDKESAEDKKRNPILKYINKPKSTSQSTFHVPLSPGEVKPGNVRNIIQHFENNQQYDVPELNLQRLSTGSFPEDLLGSDSIRSEVKLGRSESLKGREELKKSRKLDNVPRSRSDVDMDAAAEATRLHQSASSSASSLSARSLDNPTPPYTPKLCRRSMESPNLGLTGELLLSNLQEDDLGHLSDLDPEPDAQNWQHTVGRDVALHLPPKERARQEVIHELFVTEASHLRVLRVLDLVFYQKMRKENLLTPDELWLVFPNLPELIEIHNSLAESMKRVREEGPIIKEIGDLMLLQFDGASGEEVRMEAAKFCSYQTSALELIKAKKRKETRFSLFMQEAESNPQCRRLQLKDLIVSEMQRLTKYPLLLENVLKHTDSGTQEHEKLYRCRDRCRDILKYVNESVKQAENEHRLAEYQKRLDASHLERSNNPLAGEFKNLDLTTRRMIHEGPLTWRVTKDKNIDLHVLLLEDLLVLLQKQDEKLVLKCHSKTAMVSTDTKQTFSPVIKLNSVLVRSVATDKRALFIICTSDLGPQIYELVALTSSEKNTWKDLLEEATNGAMRNPPGVTKRKSQDINRPTSASMVLQRAHMPKVLAEGVLRHNSEDPQSGDESAGEFLGHQKPDVVLEESEREEDEEDEARTPTLLPIPSKEEDAGGGTRGLDSSNHIPYQWPAVTEGLAESALDDVHNLRQLILKNMLHHGHSEYNSCSAPVAEPEDDLTPTPSIIGGHGHPWDTMTDAVLENLGGDQTTEEKETSVPPSVSSSESSRSVLNFPHGDRPGSRDGEETAWSLVERYPQSSSVHQNQSEQLHCEDHSNQSYRVVRTAEVVGNYDVIPLPDSSQSQSVLQEAGGGAIVDGNFFYVTASTASAASAKEDNEVSESQMNSDRAGLSFLESQESNHTPTYQPELATPPSRNTERDPLSSDLLPPKLVIQEVDIIFRTIEQLTAKLHRLKVVESAHQELLRSLGRSATEDHYHEREHMAEKDNQAQKHQGSNLNSSSLFFVKNSISVSLHRDGNTDPQEDAINLAMGI

Target ORF information:

RefSeq Version	XM_018233487.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis rho guanine nucleotide exchange factor 11-like (LOC108700359), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018233487.1

ORF Insert Sequence:

ATGAGCATCA GTTCCGCACA GACTTCAATA GACCGATCCG CTTCTAAACG CTATCCTCGT 
TTGCCAAGGT TGAGTAGCCT GTCATCGGCT AGTGACGGAT CTGCCGAGCG AAGGTCTCCT 
GGACACCAGC GTCAGCCTTG TAACGAGAGT AACGAGTCTA CAGGCCTGGT CCAGCGCTGT 
GTCATTGTTC AAAGGGACCA AAATGGATTT GGTTTCACGG TCAGCGGAGA TCGAATTGTT 
CTGGTTCAGT CTGTACGTCC AGGTGGGGCA GCAATGCGAG CTGGGGTGCA GGAGGGAGAC 
CGGATTGTGA AGGTCAACGG CACGCTGGTG GCTAACAGCT CACACATCGA AGTGGTCAAG 
CTGATTAAAT CCGGTGCTTA CGTGGCTCTC ACTCTCCTGG GTTCTCCACC TCCATCCATG 
GGCATGTCTG GGTATCAGCA AGACTTGAGC TCCTCTGTGT CCCCACGTAA CATATTAGTG 
TCGCCAATTC CTCCACCTCC ACCACCTTTG CCGCAGTCGC ATCACATCAC AGGACCTAAA 
CCCTTACAGG ACCTCGATTT CCAAAAGCAT GCTACGGAAA TTCTTTGGAA CATGTTAAAG 
CAGGAGGAAG CAGATCTACA GAATCTGTGT GATGTGTATA ACAGAAGCCC TAACCCCAGC 
CTGCAGGAGC AGATAGAGAG GGCCAGGAAG AGGGTGCATC AGCTGCAGCA TAAGATAAGG 
CCAGAAATAC CCAGTACTGG GTATTCCATG AATCGGAATT CTGTCCTGTC AGACCTCGGA 
ATGGACAGCT CCCACACCTC CCCAATCATC CCAACGAAAC TGTTTCACCA CCACCATCGG 
CGGCACGGGT CAGACACCAC CTTTATTGCC ACGTCTGAGC AGGTATCAGA TCATAGGAGG 
TCTACGATAA TCGGGCCAGA AGAGGACTGT GACCATGGAT ATTACATGAC AGAGCAGTCA 
GATGGTCTCT TCCAAGATCT GGAAAAGTTA AAGACCCGGC CAGCCCATAT GGGGGTGTTT 
CTACGTTATA TCTTCTCCCA GGCTGATCCA AACCCACTGC TCTTTTACCT GAGTGCTGAG 
GCCTGTCAGC ATTTGAGTAC AACAGACGCC TTCACATTGA GGAAAGAAGT CTGGAACATC 
TTCCTTGACA AAAGTGCACC ACTGAGGGTT AAAATCCCTG ATCAGCTACT GAATGAAATT 
GAATCCCGTT TGCGGAACTC GGAAGATATC CGTGGTGTGT TTAGTGAAGC CCAGGAGGCG 
ATGCTGCCAG AGATACAGGA GCAGATACAG GATTACAGGA CGAAAAGGAC CATGGGATTA 
GGGAGCCTGT ACGGGGAGAA TGACCTGTTC GACCTTGAGA TTGACCCTCA CAGAGAACGT 
CATGTTGCTG CTAAACAGAT TGCACAGCTG GAGGACATAC TATCAAAGTA TGAGAAAGAG 
ATCAGCTCGC CCATGGCCTT TGCCTTGAGC ATGTATGTGA AGTACGCTGG GATCCACACT 
CAAGATATCC GTCCCGCCAG CATCTCCGAA AAAAGCATCC CCGACAGGGA AAAGTGGCTC 
CCGTTCTTCC CTAAGAACAA GAAAAGCAGC AACGCCAAAA AGGATAAAGA GTCGGCTGAG 
GACAAGAAGC GCAACCCCAT CCTTAAGTAT ATTAACAAGC CCAAGAGTAC GTCTCAGAGT 
ACATTTCATG TTCCTTTGTC CCCTGGTGAA GTTAAACCCG GGAATGTGAG AAACATTATC 
CAGCACTTTG AAAACAACCA ACAATATGAT GTTCCCGAAC TTAACCTACA GCGGCTTTCA 
ACCGGGAGCT TCCCTGAAGA TCTACTGGGC TCTGACAGCA TCAGGTCGGA GGTGAAACTG 
GGCCGGTCAG AAAGCTTAAA GGGACGGGAA GAGTTGAAGA AATCCCGCAA ATTGGACAAT 
GTTCCTCGAT CTCGCAGTGA CGTAGATATG GACGCGGCAG CTGAGGCTAC ACGGCTACAC 
CAGTCGGCTT CATCCTCTGC ATCCAGCCTA TCCGCAAGGT CATTGGATAA TCCTACCCCA 
CCCTATACTC CCAAATTATG TCGAAGGAGC ATGGAGTCGC CCAATTTGGG GTTGACTGGA 
GAATTGCTGC TCTCCAACCT GCAGGAGGAC GACCTGGGTC ACCTCTCTGA CCTTGACCCT 
GAGCCCGACG CACAGAACTG GCAGCATACG GTGGGTCGGG ACGTGGCGTT GCACCTGCCA 
CCCAAAGAGA GGGCACGGCA AGAGGTGATA CATGAGCTGT TTGTGACAGA AGCCTCCCAC 
CTTCGGGTCC TACGAGTTCT GGATCTCGTT TTCTACCAGA AGATGCGCAA GGAGAACCTG 
CTGACCCCAG ATGAGCTCTG GCTTGTCTTC CCCAATCTCC CAGAACTGAT TGAGATCCAC 
AACTCGCTGG CAGAGTCAAT GAAGCGGGTG AGAGAAGAAG GGCCAATCAT CAAAGAGATC 
GGTGACCTCA TGCTTTTGCA GTTTGATGGA GCTTCAGGGG AGGAAGTCCG AATGGAAGCA 
GCCAAGTTCT GCTCGTACCA GACATCTGCC CTTGAGTTGA TCAAAGCCAA AAAGAGGAAA 
GAGACCCGGT TCAGTCTCTT CATGCAGGAA GCTGAAAGCA ACCCCCAGTG CCGACGACTG 
CAGCTGAAGG ACCTGATTGT GTCTGAGATG CAACGGCTGA CCAAGTACCC TCTGCTGCTG 
GAAAACGTCC TTAAGCACAC AGACTCCGGG ACTCAGGAAC ATGAAAAGCT GTACCGTTGC 
CGTGATCGGT GTCGAGATAT TCTGAAATAT GTCAACGAGT CCGTCAAACA GGCAGAAAAT 
GAACATCGAC TGGCTGAGTA TCAGAAGCGA CTGGACGCAA GCCACTTGGA GCGGTCGAAT 
AATCCTCTAG CTGGAGAGTT TAAGAACTTA GACCTGACCA CAAGGCGAAT GATACATGAG 
GGGCCTCTCA CCTGGAGGGT AACAAAAGAC AAGAATATAG ACTTGCATGT TCTCTTGCTG 
GAAGATCTCC TCGTATTACT TCAGAAGCAG GATGAGAAGC TGGTGCTCAA ATGCCATAGC 
AAGACCGCCA TGGTCTCCAC CGACACCAAA CAAACCTTTA GTCCGGTCAT TAAGCTGAAT 
TCTGTCCTTG TTCGCTCGGT GGCTACAGAT AAAAGAGCCC TGTTTATTAT CTGCACTTCA 
GACTTGGGAC CTCAGATCTA CGAGCTGGTG GCACTGACAT CCTCAGAGAA AAACACGTGG 
AAGGATTTAT TGGAAGAAGC AACCAATGGT GCCATGAGGA ATCCTCCCGG TGTCACCAAA 
CGGAAATCCC AGGACATCAA TAGACCAACC TCTGCCAGCA TGGTCCTACA GAGAGCCCAC 
ATGCCCAAAG TCTTGGCAGA AGGAGTTCTA AGACATAACA GTGAGGACCC TCAATCAGGT 
GATGAGAGCG CTGGGGAATT CTTAGGCCAC CAAAAGCCAG ATGTCGTCCT AGAGGAATCG 
GAAAGGGAGG AAGATGAAGA AGATGAAGCT CGGACACCTA CCCTGCTGCC TATCCCATCT 
AAAGAGGAGG ATGCCGGAGG TGGGACACGG GGGTTAGACT CTTCCAACCA TATTCCCTAC 
CAGTGGCCTG CAGTGACTGA AGGGCTGGCT GAGTCTGCAC TGGATGATGT TCATAACCTC 
CGCCAGTTGA TCTTGAAGAA CATGCTCCAC CACGGCCATT CCGAGTACAA CTCTTGCTCT 
GCCCCAGTGG CTGAGCCTGA AGATGACCTC ACGCCTACGC CTTCCATCAT TGGAGGACAC 
GGGCACCCAT GGGACACTAT GACAGACGCA GTATTAGAAA ATCTAGGCGG AGATCAGACT 
ACTGAAGAGA AGGAGACCTC TGTTCCACCA TCAGTTAGCA GCTCTGAGAG CAGCAGGTCT 
GTTTTGAACT TTCCTCATGG AGATAGACCA GGTTCCAGAG ATGGGGAGGA GACTGCTTGG 
AGTTTGGTTG AACGCTACCC ACAAAGCAGT TCTGTTCATC AGAACCAATC AGAGCAACTA 
CACTGTGAGG ACCATAGTAA CCAAAGCTAC AGAGTTGTTA GAACAGCTGA GGTGGTGGGA 
AATTATGATG TCATACCGTT GCCAGACAGC AGCCAATCAC AGAGTGTGTT GCAGGAAGCT 
GGCGGTGGAG CTATTGTAGA TGGAAATTTC TTTTATGTAA CTGCGTCTAC GGCTTCTGCA 
GCTTCTGCAA AGGAAGATAA TGAAGTCTCA GAATCCCAAA TGAACTCTGA CCGGGCGGGC 
TTGTCCTTCT TGGAATCTCA AGAGTCAAAC CACACGCCAA CTTACCAGCC AGAACTTGCC 
ACCCCACCAT CAAGGAATAC AGAAAGAGAT CCACTTTCAT CGGATCTCCT GCCCCCAAAA 
CTTGTCATTC AGGAAGTAGA TATTATCTTC AGGACGATAG AGCAGCTGAC GGCCAAACTC 
CACAGGCTGA AGGTAGTAGA GTCAGCCCAT CAGGAGCTTC TGCGATCCTT AGGAAGAAGT 
GCCACAGAAG ACCATTACCA TGAGAGAGAG CACATGGCAG AGAAGGATAA TCAAGCTCAG 
AAGCATCAGG GGTCCAATTT GAACAGCTCC TCTCTATTCT TTGTGAAGAA CAGCATATCG 
GTCTCGCTTC ATCGAGATGG CAATACAGAC CCACAGGAAG ATGCTATAAA TCTGGCCATG 
GGAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC108700359 cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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