LOC108698614 cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC108698614
Entrez Gene ID	108698614
Full Name	spectrin beta chain, non-erythrocytic 4-like
Gene Type	protein-coding
Organism	Xenopus laevis(African clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018230231.1	XP_018085720.1	spectrin beta chain, non-erythrocytic 4-like

Pathways from BioSystems
Gene Ontology

The following LOC108698614 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC108698614 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAf42028	XM_018230231.1 Latest version!	Xenopus laevis spectrin beta chain, non-erythrocytic 4-like (LOC108698614), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC108698614 ( XM_018230231.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018085720.1 Xenopus laevis spectrin beta chain, non-erythrocytic 4-like (LOC108698614), mRNA.

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CloneID	OAf42028
Clone ID Related Accession (Same CDS sequence)	XM_018230231.1
Accession Version	XM_018230231.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7638bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1474214400000
Organism	Xenopus laevis(African clawed frog)
Product	spectrin beta chain, non-erythrocytic 4-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030738.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATG TTAGTTCAGA TCTTGATAAC ATGGAAGTGC AAACACCCCC CAATAATAAC 
AATCGATGGG AACAAACAGA GCGTCCATGG GATGAGCCAG CAGCCACAGG GAAGCTGTTT 
GAATGTTCAC GAATTAAAGC TCTGGCAGAT GAACGTGATG CCGTCCAGAA AAAGACTTTC 
ACTAAGTGGA TAAATTCTCA TCTTTCCAAA GTGCCATTGC GTGTTAATGA CTTGTACACT 
GACCTCCGAG ATGGCTACAT CATCACCAAA CTGCTTGAGG TGCTATCTGG AGAGCAGTTG 
CCTAAACCCA CTAGAGGTCG GATGCGAATT CATTATCTGG AGAATGTGGA CAAGGCACTG 
CAATTCCTAA AGGAGCAACG CGTGCACCTG GAGAATGTTG GATCTCATGA TATTGTTGAT 
GGAAATCACC GCTTAACTCT AGGCCTTATC TGGACCATCA TCCTCCGCTT TCAGATCCAG 
GTTATAAAGA TAGAAACAGA GGACAGCCGC GAGACAAGAT CAGCAAAGGA TGCCCTTCTC 
CTTTGGTGCC AAATGAAGAC ATCTGGATAT CCGGAAGTCA ATATCAAGAA CTTTACCACC 
AGCTGGAGAG ATGGGCTTGC GTTTAGTGCT CTGATTCACA GACACAGACC AGATACTATA 
GACTTCAACA AGCTGACCAA ATCAAATGCC ACATACAACC TTCAGCAGGC ATTTAATACT 
GCTGAACAAC AACTGGGCCT TACCAAACTT TTGGACCCTG AAGATGTCAA TATGGAGCAT 
CCAGATGAAA AATCCATCAT CACATATGTG GTATCTTTTT ATCACTACTT CTCCAAGATG 
AAGGCACTAG CTGTGGAAGG CAAGCGGATT GGCAAGGTAC TTGACAAGGC TATTGAGACT 
GAGAAAGATA TCATCAGGTA CGAGGTATTG GCTTCTGAGC TCTTGGAATG GATTGAGAGA 
ACAATATCCA TAATCACCAA CCAGAAATTT GCTAACTCAC TGCTGGGTGT TCAGCAGCAA 
CTCCAGGTCT TCACTACCTA CTGTACCACT GAAAAGCCCT GCAAGTTCCA GGAAAAAGGA 
AATTTGGAGG TTTTGCTATT TAGTATTCAG AGCAAGATGA GGGAAGAGAA ATGTAAACTT 
TATGTCCCAA GTGAAGGCAA AAACATTTCT GACATCAATA AGGCCTGGGC AAACTTGGAG 
AAAGCAGAGC ATGAGCGGGA AGTAGCTTTG CGTAATGAAC TCATCCGTCA GGAGAAACTG 
GAGCTGCTTT CTCAACGCTT TGATCAGAAA GCTTCCATGA GGGAGGCATG GCTAACTGAG 
AACCAGCGCC TGGTTTCCCA GGATAACTTT GGTTATGACC TCCCTGCCGT GGAGGCTGCA 
ATGAAGAAAC ATGAGGCGAT TGAAGCTGAT ATTTCCTCAT ATGAAGAACG AATACAGGTA 
GTGGCTGATC TGGCTCAAGA GTTGGAGGCT GAGGGATATT ATGATATCTG TAGGATTTCT 
GCCCAAAAGG ACAACATACT ACGTCAGTGG AACCTTCTTA AGGACATGCT GAACTCCCGA 
AGAGGGAGGC TGGAGCAGAA CTTGTCCTTG CAGAAAATAT TCCAGGAAAT GGTTTATATG 
ATTGACTGGA TGGATGAAAT GCAGGTCCCT TTATCTTCCA AAGACTTTGG AAAACACTTG 
TTGGAAGTGG AGGACCTGCT GCAGAAACAC TCTTTACTGG AAGCTGACAT TGCTGCTCAA 
ACTGAGAGGG TTAATTCTCT GAACCAAGCT GCCCTCAAAT TCACTCAGAT GACTGATTAC 
CAGCCCTGTG ACCCTCAGGT CATTTGCAAC CGTGTGGAAC ATGTCAAGAG TTGCTTAGAC 
AGTCTGCGCC AGCTAGCTGA GAAGCGCCGC GGGGAACTGG AGGAGTCACG TCGCCTGTGG 
TGTTTTCTGC AGGAAGTGGA GGAGGCTGAA GGCTGGATCT GTGAAAAAGA ACATATCTTG 
TCTGCACCAC AGAGCTATGG AAAGGATCTA AGTAGCGTTC TTCGTCTTCT AAGTAAACAT 
AAGATCTTGG TAGGGGAACT AAGAGGCAGG CATGCCATGT TACAACAGAC CCTCCAGAAG 
GGAGAGCAGG TGCTTTCCAA GAGACGAAAA GGAATGTCTG GGCTTCGTCA GCGAATGGAG 
CAGGTGAGAC TTAAGTGGGA AAACCTAGAG AAGGCATGTG ATAACCACCA GCAGAAGCTT 
CAGGAAGCTT CCAGCTTCTA CCAGTTCAGC GCTGACACAA TGGAGTTAGC AGGTTGGCTG 
CAGGATACTT ACCGCTTAGT CTCCAGTGAC GACTTTGGTC ATGATGAGTA CTCAACACAA 
TCCTTGGTAA AGAAACACAG AGCAGCAACT GTGGAAATCG AGAAACACAA AGTTGCTGTG 
TTGGGTCTCA GACAGCAACT GTCAGAACTG GGTGCAGAGT ATAGGGATCA GGTTGACGTG 
CAGATTACTA TTGTGGAGGT GGAGCAGCTC TACGGAGAAG TGGTGGAAGT ATCTGCCCTC 
AGGAACCAAT GGCTTCAAGA TGCCTTGGAT GTTTATAAGA TGTTTGGTGA GGTAAACTCA 
TGTGAGGTAT GGATTGATGA GAAAGAGCAG TGGTTGATGA AGATGGAAAT TCCTCAGCGA 
CTTGAAGAGG TTGAGGTTGT TCAGCACAGA TTTGAGAGCC TAGACCAGGA GATGAACAGC 
CTAATGGGGC GTGTGCTGGA TGTAAATCAG ATTGTACAGC AGCTTCTTGA GAGTGGTCAT 
CCCAGTTCTT CTGAGGTTCG TGCTTGCCAG GACCACCTAA ACAGCAGGTG GAATAGAATT 
GTGGAGTTGG TGGAACAAAG GAAGGAACAA TTAAGTTCCG TTCTCCGTCT CCAGAATTAT 
TTCCTGGAAT GTAATGAAGT AAAATCCCAG ATTCGGGAAA AAAGAAGAGC TATGGAGACT 
CCACAGTGTG GAAGTGGTGA GCTAGGTGGC GTGTTGTCTC TTCAGAGGAA ACTTTCCAGT 
ATGGAGGCTG CTTTGTTGGC TCTGGAACCC AAACTTGTTC AGCTTCAGCA AGATGCAGAA 
ATTCTTGCCA CCAGCCATCC TGCTGAAGGA ATGGATGTAC TTGTGCATTT TGAGGAAATC 
AGTGGAGAAT GGGATGCCTT AAAGAGGACT CTTCAGGGGT GTGAGGACTC CCTGACAGTT 
GCCAACAAGC TGCAACAGTT CATTCAGGAC CTAGACAACT TCCTTACTTG GTTGGTAAAA 
ACACAGACAG CTGTGGCTGC TGAAGAACTT CCATCAAATT TAACAGAAGC TGAGAGACTT 
CTGGGACTTC ATGGTTCCCT TAAAGAAGAG ATCAGCCGGT ATGAGGAAGA TTACAACAAA 
ATTCAGGCAG TCAGTGACCT TTTAAGTCTG GAAGAGGCAG ACCTTCCATA TCTATCACTG 
CAGCAATGGC TACAGAAGTT GGAGGTGGGA TGGAGCAAGC TGCTGCAGAT GTGGGAGAAC 
AGAAGGGAAG TTCTTGTTCA GGCACACATT TTCCATCTTT TCCTAAGAGA TGTTAAACAA 
GCTGAAGCCT GTCTAAACAC ACAGGAATCC ACATTGGCTC ATGTTGAGTT GCCAAACACA 
GTCGAAGGAG TGGAAGCAGC AATAAAAAAA CATAAAGATT TTCTGACCAC TATGGAGCTA 
AATGTGCAGA AGGTTACAAT AGCAGTGGAG GCCGGAGAGA GCCTCATACG ACTTGGAAAT 
GTGTTTGCTG ATCATGTTCA GGAACGGATC AATGCCATCC AAAAGAGGAG CCAGCGTAAT 
ACGGCACTTG CTCAAAAATG GCTGCAGAGA CTGAGAGACC ATCTGCAGCT GCAACACTTT 
ATCCAAAACT GCAATGAGCT GGATGCCTGG GTCCATGAGA AAACGCTGAT GGCTCGAGAA 
AGCTCGCGGG ATCTGTCTCA GAAACCCCAT AAGAAATGGC TAAAGCACCA GGCTTTTATG 
GCTGAGCTCT CACAGAATAA AGGTTGGCTT GAGAAGATAG AAAAGGAAGG TCAACAGCTA 
ATCCACGAAA AGCCAGAGTT GGCTGGAGTG GTGAAAAAGA AGCTTGAGGA GATTCGACAG 
TGTTGGGTTG AACTGGAGTC AAGTACCCAG TCCAAGGCCC AGCAACTTTT TCAAGCCACA 
AAGGCAGACC ATGTGATGCA GAACTACTCA GAACTCGGCA AAAGGTTATA CCATTTAGAG 
GAACAGTTAC AGCCAATGGA TTCAGTACCT GATGTTTCCA GTGTTAACAC TCAACTAAAA 
AAGCTTGAGA CAATGGAATC ACAAATGGAA GAGTGGTACA ATGAAGTTGG AGAACTGCAA 
GCACAGATTT CCTCTCTTCC TCTGGAAGTG GCAAGTCAAA AGGCAGTGGA AGAGAAGCAG 
AATGAGGTGG GAACCAAAAT GGTTCGCCTG ATTGAGCCAC TGAAGGAGAG GAGAAGGATT 
CTTTTGGCTT CTAAAGAGTT GCATCAGGTT ACTCGAGACC TGGAGGATGA AATTTTCTGG 
GTGCAAGAGC GACTGCCTCT TGCAACTTCA AAGGAACATG GGAGCAATCT TCAAACTGTG 
CAGCAACTCA TCAAGAAAAA CCAGAACCTG CGACGGGAGA TTCAGGTGCA CTCCCCTCGG 
GTAGATGATA TTCTCGAAAG GGCAGGGGCA ATAGCCAGCA TTCGAAGTCC AGAGGCAGAG 
GGAGTGCGTC TTGGGTATGA GAGCCTCCAG GAGATGTGGA ATGTGCTAAG AGAGGAGACA 
GAAAGAAGGC AGCAGCTACT CGACATTACC TACCAGGTGC AACAGTATTA TTTTGATGTA 
GGAGAAGTGG AGACCTGGCT GAGCGAACAG GAACTTCTTA TGATGAATGA TGAGAAGGGG 
AAGGATGAAC AGAGCACTCT GCAGCTCCTT AAGAAACACC TCATGCTGGA ACAGACTATT 
GAGAATTATG AGGAGACAAT AGCTCAGCTC TCTAGACAGT GCCGTACCCT TCTGGATCTA 
GGACACCCAG ACAGCGAGCA GATAAGTAAG CGTCAGTCTC AGATTGATCG GCTCTACGTG 
TCTCTGAAGG ACCTGGTGGA TGAGCGGAAG GGACGCCTGG AGCAGCAGTA TTGGCTCTTC 
CAGTTAAGTC GTGAGGTGGA TGAGCTGGAG CAATGGATAG CAGAGAAAGA AGTGGTGGCT 
GGATCTCCAG AGCTTGGACA GGACTATGAG CATGTGACTC TTCTTCAGGA GAAATTCACA 
GAGTTTGCCA CTGAGATAGG CATCATTGGA CAAGAACGCA TCTCTGCAGT AAATCAGATG 
GTGGATGAGC TCATTGACTT TGGCCATTCA GATGCAGCTA TTATTGCAGA ATGGAAAGAT 
GGTGTTAATG AGGCTTGGGC TGATCTGCTG GAATTGATGG AAACCAGATC TCAGATGTTG 
GCTGCTTCTC ATGAGCTGCA CAAGTTTTTC AATGACTGCA AAGAAGTGTT AAGTCAAGTT 
GAGGAAAAGA AGCAGAGGTT GCCTGAAGTA AAGGCCCAAG AAGCCAAGTT ATCTGCTGGA 
TCCTTGCAAA GGATACTCAG CTCTTTTGAA CATGATATTC AAGTTCTTGT TACTCAGGTA 
CGTCAACTGC AGGAAAATGC AGCTCAACTG CGCACTGTTT ATGCTGGTGA AAATGCTGCT 
GCTATTGTGA CTAGTGAGCA GGAAGTAATG CGAGCATGGA AAGAGCTTCT GACATCTTGC 
GAGGACTGCA GATTGCAAAT AACTAGTACC AGTGATCGTA TTAATTTCCT CAATCTTGTG 
AGAGACCTGA TTTCCTGGAT GGAAAGCATT ACTTGCCAGA TTGGCACAGG AGAAAAGCCC 
AGAGATGTGT CATCTGTGGA AGTCTTAATG AGTTACCATC AAGGGGTAAA GAGTGAGATT 
GAGGCAAGAA ACAAGAGGAT TTCAGACTGT GTGGAGCTTG GAAAGACCCT GGTCCTGAAC 
AAGAGACCAT CTTCAGATGA GATTAAGCAG AGCTTGGACA GGTTAATGGC AAAGAAAAAG 
GAAATGTTGG AAAAATGGAA CAAACATTGG GAGTGGCTTC AACAAATGTT GGAGGTTCAC 
CAGTTTGCCC AAGAAGCTGG AGTAGCAGAT GCCTGGCTGA CTGCTCAGGA ACCTATTGTT 
AAAAGCCCTG AGCTTGGCAA CAGTGTGGAT GAGGTGGAGC AGCTCATCCG AAGACATGAG 
GCCTTCCGCA AAACAGCTGC AGCTTGGGAG GAGAGGTTTA GTTCGCTGAG ACGCCTGACT 
ACATTGGAGA AACTAAAAGC AGAGCAAAAT AAGCAGCCTG CAACACCTCT TCTTGGCCGG 
AAAGTTTTTC CAGACACAAC TGATTATTCT ACTCGCCCTT CCCCTCTTTC ACATCAAACC 
ATATATGAGA ATTCTGAGAC AAAATTGGAT CAAAAAGAAT CTCTTCCCAT CCCTGATGGG 
GGTCAACAGG AGATCCGAAG GGTAAGAACT GACAAGGGAG AGACACGGGT TGGATATGCT 
CGACAGGATA TAAAGCCAGA ACATCTACAG CCAAAGATGG ACCATGTGGA GACCAAGGTA 
GTAGATAAAC CATTGCCAAC GCATGATGTA GAAAGGGCAG AGAAGTCTGA AATCAAAGTT 
GCACAGGGAA TGGCTAGCCT GACTGTGGAA AAACCAGAAA TCAAGGGAAG TCGTCATCAA 
GAGAGGAGGA GGGACAGGCA TGAAAGAAGA TTGGAACGGC AAGAGTCTAG TGAGCAGGAA 
CCCCCAAAAC AAGAGCCACC AGAGAGAAGG CGAGATCGTC ATGAGAAGCG ATTGGAGAGG 
CAGGCATCCA GTGAGCAGGA AACCCCTAAA CAAGAGCAGT CTGAGAGGAG AAGAGACAGG 
CATGAGAGGA GAACAGAAAG ACAGGATACA ATCGAACAAG ACAATACAAG GACTGAACCC 
GAGAAGCAAG TAGGAAAAGC TACTTTGGCA GATATCGTGG AGCAGCTACA AGAGAAAGAA 
GCTTTCCAGA ACAGCCCTGG TGGCCGAGAT TCTCCTGCCC GTTTAGCCAA TGGGGTAGAT 
AAACTTCCTG AAAGAACAAC AAGACCAGAT CGTCCACGGG CCAGAGATCG CCCAAAGCCA 
AGGAGAAGAC CTCGACCCAA AGATCCTAAC CAACCTCCTG GTGAGGCACG AAGGTCACGT 
TCAGCCCCAG CACAGAGCAG TGCTCCTCCT CCACCACCTC CCACCCACAC TGTTCAACAA 
GAAGGATATC TGTTCAGGAA ACATGAATTG GATGGGCCAA GTAAAAAGGC TTCAAATAGG 
TCATGGGTAA ATCTTTACTG TGTATTAAAC AAAGGAGATA TCGGGGTCTA CAAGGATGCA 
AAGAGTCAGA GCTCAGGGGC CACACATGGT GGTGAGCCCC TACTGAACTT GCTTGGAGCC 
TCCTGTGAGG TAGCCAGTGA ATACAAAAAG AAGAAATTTG TGTTCAAACT CAGGTCAACA 
GATGGTAGGG AATCCCTCTT TCAAGCAAAA GATGAGGAGG AGATGAAAAA CTGGATTACT 
GCGATAACCA CATGTGTCTC AGAACATGCA GAGATTGCCC GGTGGAGCCA GAGTCTTCTC 
ACTACCTCGT CCACCGATGA AAGTAACATA AAGCGTGAGT CTGACCGTAG ACCCAGTGGA 
GGTGGTCGGA AAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018085720.1
CDS	173..7810
Translation	MANVSSDLDNMEVQTPPNNNNRWEQTERPWDEPAATGKLFECSRIKALADERDAVQKKTFTKWINSHLSKVPLRVNDLYTDLRDGYIITKLLEVLSGEQLPKPTRGRMRIHYLENVDKALQFLKEQRVHLENVGSHDIVDGNHRLTLGLIWTIILRFQIQVIKIETEDSRETRSAKDALLLWCQMKTSGYPEVNIKNFTTSWRDGLAFSALIHRHRPDTIDFNKLTKSNATYNLQQAFNTAEQQLGLTKLLDPEDVNMEHPDEKSIITYVVSFYHYFSKMKALAVEGKRIGKVLDKAIETEKDIIRYEVLASELLEWIERTISIITNQKFANSLLGVQQQLQVFTTYCTTEKPCKFQEKGNLEVLLFSIQSKMREEKCKLYVPSEGKNISDINKAWANLEKAEHEREVALRNELIRQEKLELLSQRFDQKASMREAWLTENQRLVSQDNFGYDLPAVEAAMKKHEAIEADISSYEERIQVVADLAQELEAEGYYDICRISAQKDNILRQWNLLKDMLNSRRGRLEQNLSLQKIFQEMVYMIDWMDEMQVPLSSKDFGKHLLEVEDLLQKHSLLEADIAAQTERVNSLNQAALKFTQMTDYQPCDPQVICNRVEHVKSCLDSLRQLAEKRRGELEESRRLWCFLQEVEEAEGWICEKEHILSAPQSYGKDLSSVLRLLSKHKILVGELRGRHAMLQQTLQKGEQVLSKRRKGMSGLRQRMEQVRLKWENLEKACDNHQQKLQEASSFYQFSADTMELAGWLQDTYRLVSSDDFGHDEYSTQSLVKKHRAATVEIEKHKVAVLGLRQQLSELGAEYRDQVDVQITIVEVEQLYGEVVEVSALRNQWLQDALDVYKMFGEVNSCEVWIDEKEQWLMKMEIPQRLEEVEVVQHRFESLDQEMNSLMGRVLDVNQIVQQLLESGHPSSSEVRACQDHLNSRWNRIVELVEQRKEQLSSVLRLQNYFLECNEVKSQIREKRRAMETPQCGSGELGGVLSLQRKLSSMEAALLALEPKLVQLQQDAEILATSHPAEGMDVLVHFEEISGEWDALKRTLQGCEDSLTVANKLQQFIQDLDNFLTWLVKTQTAVAAEELPSNLTEAERLLGLHGSLKEEISRYEEDYNKIQAVSDLLSLEEADLPYLSLQQWLQKLEVGWSKLLQMWENRREVLVQAHIFHLFLRDVKQAEACLNTQESTLAHVELPNTVEGVEAAIKKHKDFLTTMELNVQKVTIAVEAGESLIRLGNVFADHVQERINAIQKRSQRNTALAQKWLQRLRDHLQLQHFIQNCNELDAWVHEKTLMARESSRDLSQKPHKKWLKHQAFMAELSQNKGWLEKIEKEGQQLIHEKPELAGVVKKKLEEIRQCWVELESSTQSKAQQLFQATKADHVMQNYSELGKRLYHLEEQLQPMDSVPDVSSVNTQLKKLETMESQMEEWYNEVGELQAQISSLPLEVASQKAVEEKQNEVGTKMVRLIEPLKERRRILLASKELHQVTRDLEDEIFWVQERLPLATSKEHGSNLQTVQQLIKKNQNLRREIQVHSPRVDDILERAGAIASIRSPEAEGVRLGYESLQEMWNVLREETERRQQLLDITYQVQQYYFDVGEVETWLSEQELLMMNDEKGKDEQSTLQLLKKHLMLEQTIENYEETIAQLSRQCRTLLDLGHPDSEQISKRQSQIDRLYVSLKDLVDERKGRLEQQYWLFQLSREVDELEQWIAEKEVVAGSPELGQDYEHVTLLQEKFTEFATEIGIIGQERISAVNQMVDELIDFGHSDAAIIAEWKDGVNEAWADLLELMETRSQMLAASHELHKFFNDCKEVLSQVEEKKQRLPEVKAQEAKLSAGSLQRILSSFEHDIQVLVTQVRQLQENAAQLRTVYAGENAAAIVTSEQEVMRAWKELLTSCEDCRLQITSTSDRINFLNLVRDLISWMESITCQIGTGEKPRDVSSVEVLMSYHQGVKSEIEARNKRISDCVELGKTLVLNKRPSSDEIKQSLDRLMAKKKEMLEKWNKHWEWLQQMLEVHQFAQEAGVADAWLTAQEPIVKSPELGNSVDEVEQLIRRHEAFRKTAAAWEERFSSLRRLTTLEKLKAEQNKQPATPLLGRKVFPDTTDYSTRPSPLSHQTIYENSETKLDQKESLPIPDGGQQEIRRVRTDKGETRVGYARQDIKPEHLQPKMDHVETKVVDKPLPTHDVERAEKSEIKVAQGMASLTVEKPEIKGSRHQERRRDRHERRLERQESSEQEPPKQEPPERRRDRHEKRLERQASSEQETPKQEQSERRRDRHERRTERQDTIEQDNTRTEPEKQVGKATLADIVEQLQEKEAFQNSPGGRDSPARLANGVDKLPERTTRPDRPRARDRPKPRRRPRPKDPNQPPGEARRSRSAPAQSSAPPPPPPTHTVQQEGYLFRKHELDGPSKKASNRSWVNLYCVLNKGDIGVYKDAKSQSSGATHGGEPLLNLLGASCEVASEYKKKKFVFKLRSTDGRESLFQAKDEEEMKNWITAITTCVSEHAEIARWSQSLLTTSSTDESNIKRESDRRPSGGGRKK

Target ORF information:

RefSeq Version	XM_018230231.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis spectrin beta chain, non-erythrocytic 4-like (LOC108698614), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018230231.1

ORF Insert Sequence:

ATGGCAAATG TTAGTTCAGA TCTTGATAAC ATGGAAGTGC AAACACCCCC CAATAATAAC 
AATCGATGGG AACAAACAGA GCGTCCATGG GATGAGCCAG CAGCCACAGG GAAGCTGTTT 
GAATGTTCAC GAATTAAAGC TCTGGCAGAT GAACGTGATG CCGTCCAGAA AAAGACTTTC 
ACTAAGTGGA TAAATTCTCA TCTTTCCAAA GTGCCATTGC GTGTTAATGA CTTGTACACT 
GACCTCCGAG ATGGCTACAT CATCACCAAA CTGCTTGAGG TGCTATCTGG AGAGCAGTTG 
CCTAAACCCA CTAGAGGTCG GATGCGAATT CATTATCTGG AGAATGTGGA CAAGGCACTG 
CAATTCCTAA AGGAGCAACG CGTGCACCTG GAGAATGTTG GATCTCATGA TATTGTTGAT 
GGAAATCACC GCTTAACTCT AGGCCTTATC TGGACCATCA TCCTCCGCTT TCAGATCCAG 
GTTATAAAGA TAGAAACAGA GGACAGCCGC GAGACAAGAT CAGCAAAGGA TGCCCTTCTC 
CTTTGGTGCC AAATGAAGAC ATCTGGATAT CCGGAAGTCA ATATCAAGAA CTTTACCACC 
AGCTGGAGAG ATGGGCTTGC GTTTAGTGCT CTGATTCACA GACACAGACC AGATACTATA 
GACTTCAACA AGCTGACCAA ATCAAATGCC ACATACAACC TTCAGCAGGC ATTTAATACT 
GCTGAACAAC AACTGGGCCT TACCAAACTT TTGGACCCTG AAGATGTCAA TATGGAGCAT 
CCAGATGAAA AATCCATCAT CACATATGTG GTATCTTTTT ATCACTACTT CTCCAAGATG 
AAGGCACTAG CTGTGGAAGG CAAGCGGATT GGCAAGGTAC TTGACAAGGC TATTGAGACT 
GAGAAAGATA TCATCAGGTA CGAGGTATTG GCTTCTGAGC TCTTGGAATG GATTGAGAGA 
ACAATATCCA TAATCACCAA CCAGAAATTT GCTAACTCAC TGCTGGGTGT TCAGCAGCAA 
CTCCAGGTCT TCACTACCTA CTGTACCACT GAAAAGCCCT GCAAGTTCCA GGAAAAAGGA 
AATTTGGAGG TTTTGCTATT TAGTATTCAG AGCAAGATGA GGGAAGAGAA ATGTAAACTT 
TATGTCCCAA GTGAAGGCAA AAACATTTCT GACATCAATA AGGCCTGGGC AAACTTGGAG 
AAAGCAGAGC ATGAGCGGGA AGTAGCTTTG CGTAATGAAC TCATCCGTCA GGAGAAACTG 
GAGCTGCTTT CTCAACGCTT TGATCAGAAA GCTTCCATGA GGGAGGCATG GCTAACTGAG 
AACCAGCGCC TGGTTTCCCA GGATAACTTT GGTTATGACC TCCCTGCCGT GGAGGCTGCA 
ATGAAGAAAC ATGAGGCGAT TGAAGCTGAT ATTTCCTCAT ATGAAGAACG AATACAGGTA 
GTGGCTGATC TGGCTCAAGA GTTGGAGGCT GAGGGATATT ATGATATCTG TAGGATTTCT 
GCCCAAAAGG ACAACATACT ACGTCAGTGG AACCTTCTTA AGGACATGCT GAACTCCCGA 
AGAGGGAGGC TGGAGCAGAA CTTGTCCTTG CAGAAAATAT TCCAGGAAAT GGTTTATATG 
ATTGACTGGA TGGATGAAAT GCAGGTCCCT TTATCTTCCA AAGACTTTGG AAAACACTTG 
TTGGAAGTGG AGGACCTGCT GCAGAAACAC TCTTTACTGG AAGCTGACAT TGCTGCTCAA 
ACTGAGAGGG TTAATTCTCT GAACCAAGCT GCCCTCAAAT TCACTCAGAT GACTGATTAC 
CAGCCCTGTG ACCCTCAGGT CATTTGCAAC CGTGTGGAAC ATGTCAAGAG TTGCTTAGAC 
AGTCTGCGCC AGCTAGCTGA GAAGCGCCGC GGGGAACTGG AGGAGTCACG TCGCCTGTGG 
TGTTTTCTGC AGGAAGTGGA GGAGGCTGAA GGCTGGATCT GTGAAAAAGA ACATATCTTG 
TCTGCACCAC AGAGCTATGG AAAGGATCTA AGTAGCGTTC TTCGTCTTCT AAGTAAACAT 
AAGATCTTGG TAGGGGAACT AAGAGGCAGG CATGCCATGT TACAACAGAC CCTCCAGAAG 
GGAGAGCAGG TGCTTTCCAA GAGACGAAAA GGAATGTCTG GGCTTCGTCA GCGAATGGAG 
CAGGTGAGAC TTAAGTGGGA AAACCTAGAG AAGGCATGTG ATAACCACCA GCAGAAGCTT 
CAGGAAGCTT CCAGCTTCTA CCAGTTCAGC GCTGACACAA TGGAGTTAGC AGGTTGGCTG 
CAGGATACTT ACCGCTTAGT CTCCAGTGAC GACTTTGGTC ATGATGAGTA CTCAACACAA 
TCCTTGGTAA AGAAACACAG AGCAGCAACT GTGGAAATCG AGAAACACAA AGTTGCTGTG 
TTGGGTCTCA GACAGCAACT GTCAGAACTG GGTGCAGAGT ATAGGGATCA GGTTGACGTG 
CAGATTACTA TTGTGGAGGT GGAGCAGCTC TACGGAGAAG TGGTGGAAGT ATCTGCCCTC 
AGGAACCAAT GGCTTCAAGA TGCCTTGGAT GTTTATAAGA TGTTTGGTGA GGTAAACTCA 
TGTGAGGTAT GGATTGATGA GAAAGAGCAG TGGTTGATGA AGATGGAAAT TCCTCAGCGA 
CTTGAAGAGG TTGAGGTTGT TCAGCACAGA TTTGAGAGCC TAGACCAGGA GATGAACAGC 
CTAATGGGGC GTGTGCTGGA TGTAAATCAG ATTGTACAGC AGCTTCTTGA GAGTGGTCAT 
CCCAGTTCTT CTGAGGTTCG TGCTTGCCAG GACCACCTAA ACAGCAGGTG GAATAGAATT 
GTGGAGTTGG TGGAACAAAG GAAGGAACAA TTAAGTTCCG TTCTCCGTCT CCAGAATTAT 
TTCCTGGAAT GTAATGAAGT AAAATCCCAG ATTCGGGAAA AAAGAAGAGC TATGGAGACT 
CCACAGTGTG GAAGTGGTGA GCTAGGTGGC GTGTTGTCTC TTCAGAGGAA ACTTTCCAGT 
ATGGAGGCTG CTTTGTTGGC TCTGGAACCC AAACTTGTTC AGCTTCAGCA AGATGCAGAA 
ATTCTTGCCA CCAGCCATCC TGCTGAAGGA ATGGATGTAC TTGTGCATTT TGAGGAAATC 
AGTGGAGAAT GGGATGCCTT AAAGAGGACT CTTCAGGGGT GTGAGGACTC CCTGACAGTT 
GCCAACAAGC TGCAACAGTT CATTCAGGAC CTAGACAACT TCCTTACTTG GTTGGTAAAA 
ACACAGACAG CTGTGGCTGC TGAAGAACTT CCATCAAATT TAACAGAAGC TGAGAGACTT 
CTGGGACTTC ATGGTTCCCT TAAAGAAGAG ATCAGCCGGT ATGAGGAAGA TTACAACAAA 
ATTCAGGCAG TCAGTGACCT TTTAAGTCTG GAAGAGGCAG ACCTTCCATA TCTATCACTG 
CAGCAATGGC TACAGAAGTT GGAGGTGGGA TGGAGCAAGC TGCTGCAGAT GTGGGAGAAC 
AGAAGGGAAG TTCTTGTTCA GGCACACATT TTCCATCTTT TCCTAAGAGA TGTTAAACAA 
GCTGAAGCCT GTCTAAACAC ACAGGAATCC ACATTGGCTC ATGTTGAGTT GCCAAACACA 
GTCGAAGGAG TGGAAGCAGC AATAAAAAAA CATAAAGATT TTCTGACCAC TATGGAGCTA 
AATGTGCAGA AGGTTACAAT AGCAGTGGAG GCCGGAGAGA GCCTCATACG ACTTGGAAAT 
GTGTTTGCTG ATCATGTTCA GGAACGGATC AATGCCATCC AAAAGAGGAG CCAGCGTAAT 
ACGGCACTTG CTCAAAAATG GCTGCAGAGA CTGAGAGACC ATCTGCAGCT GCAACACTTT 
ATCCAAAACT GCAATGAGCT GGATGCCTGG GTCCATGAGA AAACGCTGAT GGCTCGAGAA 
AGCTCGCGGG ATCTGTCTCA GAAACCCCAT AAGAAATGGC TAAAGCACCA GGCTTTTATG 
GCTGAGCTCT CACAGAATAA AGGTTGGCTT GAGAAGATAG AAAAGGAAGG TCAACAGCTA 
ATCCACGAAA AGCCAGAGTT GGCTGGAGTG GTGAAAAAGA AGCTTGAGGA GATTCGACAG 
TGTTGGGTTG AACTGGAGTC AAGTACCCAG TCCAAGGCCC AGCAACTTTT TCAAGCCACA 
AAGGCAGACC ATGTGATGCA GAACTACTCA GAACTCGGCA AAAGGTTATA CCATTTAGAG 
GAACAGTTAC AGCCAATGGA TTCAGTACCT GATGTTTCCA GTGTTAACAC TCAACTAAAA 
AAGCTTGAGA CAATGGAATC ACAAATGGAA GAGTGGTACA ATGAAGTTGG AGAACTGCAA 
GCACAGATTT CCTCTCTTCC TCTGGAAGTG GCAAGTCAAA AGGCAGTGGA AGAGAAGCAG 
AATGAGGTGG GAACCAAAAT GGTTCGCCTG ATTGAGCCAC TGAAGGAGAG GAGAAGGATT 
CTTTTGGCTT CTAAAGAGTT GCATCAGGTT ACTCGAGACC TGGAGGATGA AATTTTCTGG 
GTGCAAGAGC GACTGCCTCT TGCAACTTCA AAGGAACATG GGAGCAATCT TCAAACTGTG 
CAGCAACTCA TCAAGAAAAA CCAGAACCTG CGACGGGAGA TTCAGGTGCA CTCCCCTCGG 
GTAGATGATA TTCTCGAAAG GGCAGGGGCA ATAGCCAGCA TTCGAAGTCC AGAGGCAGAG 
GGAGTGCGTC TTGGGTATGA GAGCCTCCAG GAGATGTGGA ATGTGCTAAG AGAGGAGACA 
GAAAGAAGGC AGCAGCTACT CGACATTACC TACCAGGTGC AACAGTATTA TTTTGATGTA 
GGAGAAGTGG AGACCTGGCT GAGCGAACAG GAACTTCTTA TGATGAATGA TGAGAAGGGG 
AAGGATGAAC AGAGCACTCT GCAGCTCCTT AAGAAACACC TCATGCTGGA ACAGACTATT 
GAGAATTATG AGGAGACAAT AGCTCAGCTC TCTAGACAGT GCCGTACCCT TCTGGATCTA 
GGACACCCAG ACAGCGAGCA GATAAGTAAG CGTCAGTCTC AGATTGATCG GCTCTACGTG 
TCTCTGAAGG ACCTGGTGGA TGAGCGGAAG GGACGCCTGG AGCAGCAGTA TTGGCTCTTC 
CAGTTAAGTC GTGAGGTGGA TGAGCTGGAG CAATGGATAG CAGAGAAAGA AGTGGTGGCT 
GGATCTCCAG AGCTTGGACA GGACTATGAG CATGTGACTC TTCTTCAGGA GAAATTCACA 
GAGTTTGCCA CTGAGATAGG CATCATTGGA CAAGAACGCA TCTCTGCAGT AAATCAGATG 
GTGGATGAGC TCATTGACTT TGGCCATTCA GATGCAGCTA TTATTGCAGA ATGGAAAGAT 
GGTGTTAATG AGGCTTGGGC TGATCTGCTG GAATTGATGG AAACCAGATC TCAGATGTTG 
GCTGCTTCTC ATGAGCTGCA CAAGTTTTTC AATGACTGCA AAGAAGTGTT AAGTCAAGTT 
GAGGAAAAGA AGCAGAGGTT GCCTGAAGTA AAGGCCCAAG AAGCCAAGTT ATCTGCTGGA 
TCCTTGCAAA GGATACTCAG CTCTTTTGAA CATGATATTC AAGTTCTTGT TACTCAGGTA 
CGTCAACTGC AGGAAAATGC AGCTCAACTG CGCACTGTTT ATGCTGGTGA AAATGCTGCT 
GCTATTGTGA CTAGTGAGCA GGAAGTAATG CGAGCATGGA AAGAGCTTCT GACATCTTGC 
GAGGACTGCA GATTGCAAAT AACTAGTACC AGTGATCGTA TTAATTTCCT CAATCTTGTG 
AGAGACCTGA TTTCCTGGAT GGAAAGCATT ACTTGCCAGA TTGGCACAGG AGAAAAGCCC 
AGAGATGTGT CATCTGTGGA AGTCTTAATG AGTTACCATC AAGGGGTAAA GAGTGAGATT 
GAGGCAAGAA ACAAGAGGAT TTCAGACTGT GTGGAGCTTG GAAAGACCCT GGTCCTGAAC 
AAGAGACCAT CTTCAGATGA GATTAAGCAG AGCTTGGACA GGTTAATGGC AAAGAAAAAG 
GAAATGTTGG AAAAATGGAA CAAACATTGG GAGTGGCTTC AACAAATGTT GGAGGTTCAC 
CAGTTTGCCC AAGAAGCTGG AGTAGCAGAT GCCTGGCTGA CTGCTCAGGA ACCTATTGTT 
AAAAGCCCTG AGCTTGGCAA CAGTGTGGAT GAGGTGGAGC AGCTCATCCG AAGACATGAG 
GCCTTCCGCA AAACAGCTGC AGCTTGGGAG GAGAGGTTTA GTTCGCTGAG ACGCCTGACT 
ACATTGGAGA AACTAAAAGC AGAGCAAAAT AAGCAGCCTG CAACACCTCT TCTTGGCCGG 
AAAGTTTTTC CAGACACAAC TGATTATTCT ACTCGCCCTT CCCCTCTTTC ACATCAAACC 
ATATATGAGA ATTCTGAGAC AAAATTGGAT CAAAAAGAAT CTCTTCCCAT CCCTGATGGG 
GGTCAACAGG AGATCCGAAG GGTAAGAACT GACAAGGGAG AGACACGGGT TGGATATGCT 
CGACAGGATA TAAAGCCAGA ACATCTACAG CCAAAGATGG ACCATGTGGA GACCAAGGTA 
GTAGATAAAC CATTGCCAAC GCATGATGTA GAAAGGGCAG AGAAGTCTGA AATCAAAGTT 
GCACAGGGAA TGGCTAGCCT GACTGTGGAA AAACCAGAAA TCAAGGGAAG TCGTCATCAA 
GAGAGGAGGA GGGACAGGCA TGAAAGAAGA TTGGAACGGC AAGAGTCTAG TGAGCAGGAA 
CCCCCAAAAC AAGAGCCACC AGAGAGAAGG CGAGATCGTC ATGAGAAGCG ATTGGAGAGG 
CAGGCATCCA GTGAGCAGGA AACCCCTAAA CAAGAGCAGT CTGAGAGGAG AAGAGACAGG 
CATGAGAGGA GAACAGAAAG ACAGGATACA ATCGAACAAG ACAATACAAG GACTGAACCC 
GAGAAGCAAG TAGGAAAAGC TACTTTGGCA GATATCGTGG AGCAGCTACA AGAGAAAGAA 
GCTTTCCAGA ACAGCCCTGG TGGCCGAGAT TCTCCTGCCC GTTTAGCCAA TGGGGTAGAT 
AAACTTCCTG AAAGAACAAC AAGACCAGAT CGTCCACGGG CCAGAGATCG CCCAAAGCCA 
AGGAGAAGAC CTCGACCCAA AGATCCTAAC CAACCTCCTG GTGAGGCACG AAGGTCACGT 
TCAGCCCCAG CACAGAGCAG TGCTCCTCCT CCACCACCTC CCACCCACAC TGTTCAACAA 
GAAGGATATC TGTTCAGGAA ACATGAATTG GATGGGCCAA GTAAAAAGGC TTCAAATAGG 
TCATGGGTAA ATCTTTACTG TGTATTAAAC AAAGGAGATA TCGGGGTCTA CAAGGATGCA 
AAGAGTCAGA GCTCAGGGGC CACACATGGT GGTGAGCCCC TACTGAACTT GCTTGGAGCC 
TCCTGTGAGG TAGCCAGTGA ATACAAAAAG AAGAAATTTG TGTTCAAACT CAGGTCAACA 
GATGGTAGGG AATCCCTCTT TCAAGCAAAA GATGAGGAGG AGATGAAAAA CTGGATTACT 
GCGATAACCA CATGTGTCTC AGAACATGCA GAGATTGCCC GGTGGAGCCA GAGTCTTCTC 
ACTACCTCGT CCACCGATGA AAGTAACATA AAGCGTGAGT CTGACCGTAG ACCCAGTGGA 
GGTGGTCGGA AAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC108698614 cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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