Tc00.1047053511821.130 cDNA ORF clone, Trypanosoma cruzi strain CL Brener

Gene
Clones
gRNAs

Summary

Gene Symbol	Tc00.1047053511821.130
Entrez Gene ID	3548668
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Trypanosoma cruzi strain CL Brener

mRNA and Protein(s)

mRNA	Protein	Name
XM_811651.1	XP_816744.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease.
El-Sayed NM, Myler PJ, Bartholomeu DC, Nilsson D, Aggarwal G, Tran AN, Ghedin E, Worthey EA, Delcher AL, Blandin G, Westenberger SJ, Caler E, Cerqueira GC, Branche C, Haas B, Anupama A, Arner E, Aslund L, Attipoe P, Bontempi E, Bringaud F, Burton P, Cadag E, Campbell DA, Carrington M, Crabtree J, Darban H, da Silveira JF, de Jong P, Edwards K, Englund PT, Fazelina G, Feldblyum T, Ferella M, Frasch AC, Gull K, Horn D, Hou L, Huang Y, Kindlund E, Klingbeil M, Kluge S, Koo H, Lacerda D, Levin MJ, Lorenzi H, Louie T, Machado CR, McCulloch R, McKenna A, Mizuno Y, Mottram JC, Nelson S, Ochaya S, Osoegawa K, Pai G, Parsons M, Pentony M, Pettersson U, Pop M, Ramirez JL, Rinta J, Robertson L, Salzberg SL, Sanchez DO, Seyler A, Sharma R, Shetty J, Simpson AJ, Sisk E, Tammi MT, Tarleton R, Teixeira S, Van Aken S, Vogt C, Ward PN, Wickstead B, Wortman J, White O, Fraser CM, Stuart KD, Andersson B
Science (New York, N.Y.)309(5733)409-15(2005 Jul)

Comparative genomics of trypanosomatid parasitic protozoa.
El-Sayed NM, Myler PJ, Blandin G, Berriman M, Crabtree J, Aggarwal G, Caler E, Renauld H, Worthey EA, Hertz-Fowler C, Ghedin E, Peacock C, Bartholomeu DC, Haas BJ, Tran AN, Wortman JR, Alsmark UC, Angiuoli S, Anupama A, Badger J, Bringaud F, Cadag E, Carlton JM, Cerqueira GC, Creasy T, Delcher AL, Djikeng A, Embley TM, Hauser C, Ivens AC, Kummerfeld SK, Pereira-Leal JB, Nilsson D, Peterson J, Salzberg SL, Shallom J, Silva JC, Sundaram J, Westenberger S, White O, Melville SE, Donelson JE, Andersson B, Stuart KD, Hall N
Science (New York, N.Y.)309(5733)404-9(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Tc00.1047053511821.130 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tc00.1047053511821.130 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTb11227	XM_811651.1 Latest version!	Trypanosoma cruzi strain CL Brener hypothetical protein Tc00.1047053511821.130 partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tc00.1047053511821.130 ( XM_811651.1 ) cDNA ORF clone, Trypanosoma cruzi strain CL Brener -> XP_816744.1 Trypanosoma cruzi strain CL Brener hypothetical protein Tc00.1047053511821.130 partial mRNA.

Price & Availability↑

CloneID	OTb11227
Clone ID Related Accession (Same CDS sequence)	XM_811651.1
Accession Version	XM_811651.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3906bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1225382400000
Organism	Trypanosoma cruzi strain CL Brener
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001849568). COMPLETENESS: incomplete on both ends.

ATGGCAATAC CGAACCCCCG TCGCTGGAGG CGGGAGTTTG GCGGGACACG AAAGCGAAGA 
ATACGAATAG TGCTTCGTTT TCTTTTCTTT TCATTGTGTC TCTTAGTTAT CGGCGTGTTC 
CTCCTGTTGC TCCTCGCTGC GAACGAGAAG AGGACTTTTG AGAAGGAACT GACCGAGAGG 
GAGATCCCAC ACTATTCTAC GAATGAATTT TTTACGCGTG TCAGCTTCGT CATGAGTAGC 
GCCATGGCCA CGGCGTGGCG CACCTCCAGC GATCGGCTTG AATTCCCAGC GGAGATGTGG 
GTGGAGCGCG TGGCTGTTCG CCCTTCCGCT GCCACGACGG TGCCACTTCC TGCCGTGCTG 
CAGAAGGCAA CGGTGTCGGC CACACTGTAT GGGAAAAGCT CCCTGAAGCT CCAGCGCACC 
AAACGGCGAT CGTTGTACAT TCGCAGTGAC TCACCCATTT TTTTTGTGAC GCCTAACCCT 
GCATTGTCAC CCAACCCATT CAAACGAAAT ACGACGGTGG CGTTGAAGGA ATTTATATTG 
CTTTCCCTAT GGGAGGACAT CCACCGCATC GCCTACCGCA CTTCCGTCGA TCTGCTGCAA 
CATTTAGATT TGATTTTTTC ATATGCGCAA CTGGTGGAAC TACGCTGCTA TCCGCCTTCG 
CAGAAGTCTC ATTTGGATCA CCCATGCGTA CCGTCTGTTG CTGGTGTTTC TGTGCGCGAT 
GGTGTGCATG TGGGGCGGAC CCTTGGTGTT TATTTGGCCA TTGAGCCGCC CGCCCCGGCG 
ATATGGCGGC GAATGAAAGA GGCCTTTTCC GCCTTTGAGG AGTTGGCGAA TGAGTCTCTG 
AGGGAAGTAA TTGAATCCGT GAGTCATGGT TCCCCTGCCG CTCTGCAAAG ACGTGAGTAC 
GAGAGTGTAA TGGAGCACAC CGCACGCATC ACGTATTGGC GGCAAATGTG GCGTGCCGCG 
AAGAAGTCCT CCCCGGGGAA ATTGGAGCAT GCTGTTGAAA TAGGGTCCCG CAACTTTTTG 
AGTTTTAGTG AGCCACGACA TGATATGTCA CATGTGCCGA GTTTTTTGAA AAACAGTTCC 
TTGTTGCCCA GTTATCGGCT TCTGGAAAAG GATCCCTGGC ACTACGGCCT GCACAGTGAG 
ACGGCCATGG GCAAAACGGT TAATCCTAAA AAGCAATTTC TTGATTTACC ACAATACATG 
GCATGGATTG CCATCAACAC GATACTTCAG AATGGTGATT ACGATGATGA GGTGTTTTTT 
TACGGAGTGC TAAAGCCAAT TCAATATGGA AATCCGAAGT TCCAGTTACC GATTTATCTG 
GGTGTGATGG GCTGGGACTA TGACACCGTC TTTTCCCCAT GCCATCGCAA GGTGTGGTTT 
AAGGCATCCG TCATGTATTG TGCTGAAACG ACCCTTGATA AAAGCATTTA TGACTCGGAA 
GCTTTTATTT ATCGATACAC AGATATACTA TGGGATATGA CGCAGCACAT TGACAGGGAG 
CGATTCAGTC GATCTGTTCA AGTGGCGGAG AACGAGCTGC AAACAATACT TTATGGCGCC 
CCAGGGACAA CGATGGCAAC ATTTAATCAG GTAAAACGCG AAAATGACGC TGGCGTGCGG 
TGGATTATTG GGGCCTTTTT GGAACAACGA CAAATATTGC TTCGGGCTTT GGCCTCTTAT 
TTTCCTGTCA ACGAGGTCTC GCATTGCGAT CAAACAAGTG ATAGAGATGC CAAGGCGCTG 
GAGATCCACA ACAGGGAGGC ACATCTCGAA CTGATGAAAC GTCTCTCAAT TGTCGTCAAA 
GTCAAGGGAT CGCGTGTCAG CACAAGATGG GGGTTTTTAG TTGGAGGGAT ACAGTCGGCG 
TTGGGTGTCG ATCTTCCAGA AGATGAGGAG TATCAACTCC CTGATGCTGG TATTTACTGT 
GCACGCGTGT CTGCTGTGGC ATCTTTCTTC ACATCTGTCG GACTGTTGCC TCCTCCCAAA 
GGGCGTGGGA AATGGGAGGC GCCGGGTTTC TGGTTTGTTG ACATGCCATT GGTGGAGCCA 
AGCACGTCGC GTGGCAGTGC AGAGTTTGGG CTGTGGTCCT TTGATAAGAA ATTACCCATG 
CTGTACCCAA TGACAACACC TGATGCAGGG GCCACGCTTA GCATCGTTAT GCCACCTGTG 
TGGCCTCAAG ACTCCAAGGA GAAGCGTGGT GTGTTTGTTT CTCGGGTACT TTTTCCAGAG 
CAGGGTTCAG AAAACTCCCT CTTTAGATGG TCGAGGAAAA ACACGCTTAT CTTCACCATT 
GATGCCCCAA CGCAGAGGAC GATGATTGTA CGTGGCTTCA GTGCTGTACG TTTTTTTGTG 
CCAGAGAAAT CTGAGGAAGA TTCCTTTTCT GCCCAGCGTC ATGAGGGGGA AAATACAGTT 
AGGGGCCCAG GGTTGTTTGC TTCTAACAGC TCCACCACGC GGCTGCGTAT AGGTGATGTG 
GACATTGAAC AGTCGGTACC TCTCGCGTCT GTTGCGGCTC CACCACAAGC GTTATATTTG 
GCCGACGGCC TCGTGGACGC GAAGGGCGTC AGGTTTGGCG GTTCGCTTGA GAAACAGACG 
GTGCTGCGTT GCATCCCTGA AAATGCGTCG GTGGCGGAAT CGGTGGTGGG AGAGGGTTTG 
ACGCTGCGCT CGGCAGAGTG CCCCATTTTT ATTTCCGGCT CTTTTGAAAT TTCTCGAGGA 
GCGACTCTCG ATGTGGCGGC GGGTTGTGTC GTTGTGATGC GGCCTGCTGC GATGCTGCGC 
GTGACGGGGT TTCTGCGGTT CAACGGCACC GTCGCGTCGC CCATTGTGGT CACCAGCGAA 
TCGGCGGAGA ATGCGAGCAG TTGGCAAACC ATCCACGTCG ATGGCCCCAC CGCCACGTTA 
TACGCGAGTT TTACGTTTTT CACCGGCTCA GGCGTGAAGG GGATCCGGGT GGCAAACACT 
GGGAAACACC ACTCCCATTC TGCCGCCATC TCAGCTACAA ATAAGGCCTC CGTCTATCTC 
CGTCACAGTT TCTTGTTGGA GCTGCAGGGG GCAGGCATTG CGGCAGGCAA TGAGGCCGAG 
GTGCAAGTGG AGGATTCATT GGTGCAGTGG GCTCAAATGG GGATCGAGTG TGTGCAGTGC 
ACCTTCACTT CAAGGCGCTC TGTCTGGACG CACTTTCCCT CATGGGATGC AGCGTATGCC 
GATGACGACA ATGACGCCAT GTACCTTTCG GGTGGTAAAC ATCGCGTCAT TGATAGTGTC 
ATTGCCCACA CGAAGGACGA CGGGATTGAC TCTGGTGCGA TGGGTAACGG TGGCTCCCTG 
GTTGTGCACA ACACAATTGT AGAGGGTTGC ATGCATGAGG GGGTGGCTCT CTCCTCCGAT 
CCCAACGGCC GTCGTGAGGT TGTCATTTCT CACACCATCG TGCAGTATTG TCAGCAGGGC 
ATCGAGTCGG GTTATGCGTC GAAACAACAC ACAGCGTCCA TTACGAGTTC TCTCATTCAG 
AAATGCTCCG TCGGTGTTCG TTATGGCGAC AACTACCACT GGTCGCACTG CGACGGGAAA 
CTGACACTGC AGAACACAAC GCTTGCCCAG AACGAAGTGA ATGTGATGAA CTACGAGCGA 
CGGTATCAAA TACCGCAAGA CCCCGAATAC TTCCACTCCG AGGGAGTTTT GTCACAGCGT 
CACTGGAAAC TTCTTCGGGA CGCCGCACGT GATTCCGCTC TCCCGCTGGC GGTGGCGCCA 
CCGGAGGCTG TTGTCATGCT ACTGATGGAT GTGCGACGAA GAGAGCTGCC GAAAACAGGA 
TGGGAGGGCT GCAAGGACAT GTTCTTGCTA CCCGGCAACA GCACTCTTAT CGTTGATGAA 
GTAGTTGATG CGGAGGCGTT TACAGAAGAG CTGAGAGTTA CGGAAGATGT CACAGTCACC 
TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_816744.1
CDS	1..3906
Translation	MAIPNPRRWRREFGGTRKRRIRIVLRFLFFSLCLLVIGVFLLLLLAANEKRTFEKELTEREIPHYSTNEFFTRVSFVMSSAMATAWRTSSDRLEFPAEMWVERVAVRPSAATTVPLPAVLQKATVSATLYGKSSLKLQRTKRRSLYIRSDSPIFFVTPNPALSPNPFKRNTTVALKEFILLSLWEDIHRIAYRTSVDLLQHLDLIFSYAQLVELRCYPPSQKSHLDHPCVPSVAGVSVRDGVHVGRTLGVYLAIEPPAPAIWRRMKEAFSAFEELANESLREVIESVSHGSPAALQRREYESVMEHTARITYWRQMWRAAKKSSPGKLEHAVEIGSRNFLSFSEPRHDMSHVPSFLKNSSLLPSYRLLEKDPWHYGLHSETAMGKTVNPKKQFLDLPQYMAWIAINTILQNGDYDDEVFFYGVLKPIQYGNPKFQLPIYLGVMGWDYDTVFSPCHRKVWFKASVMYCAETTLDKSIYDSEAFIYRYTDILWDMTQHIDRERFSRSVQVAENELQTILYGAPGTTMATFNQVKRENDAGVRWIIGAFLEQRQILLRALASYFPVNEVSHCDQTSDRDAKALEIHNREAHLELMKRLSIVVKVKGSRVSTRWGFLVGGIQSALGVDLPEDEEYQLPDAGIYCARVSAVASFFTSVGLLPPPKGRGKWEAPGFWFVDMPLVEPSTSRGSAEFGLWSFDKKLPMLYPMTTPDAGATLSIVMPPVWPQDSKEKRGVFVSRVLFPEQGSENSLFRWSRKNTLIFTIDAPTQRTMIVRGFSAVRFFVPEKSEEDSFSAQRHEGENTVRGPGLFASNSSTTRLRIGDVDIEQSVPLASVAAPPQALYLADGLVDAKGVRFGGSLEKQTVLRCIPENASVAESVVGEGLTLRSAECPIFISGSFEISRGATLDVAAGCVVVMRPAAMLRVTGFLRFNGTVASPIVVTSESAENASSWQTIHVDGPTATLYASFTFFTGSGVKGIRVANTGKHHSHSAAISATNKASVYLRHSFLLELQGAGIAAGNEAEVQVEDSLVQWAQMGIECVQCTFTSRRSVWTHFPSWDAAYADDDNDAMYLSGGKHRVIDSVIAHTKDDGIDSGAMGNGGSLVVHNTIVEGCMHEGVALSSDPNGRREVVISHTIVQYCQQGIESGYASKQHTASITSSLIQKCSVGVRYGDNYHWSHCDGKLTLQNTTLAQNEVNVMNYERRYQIPQDPEYFHSEGVLSQRHWKLLRDAARDSALPLAVAPPEAVVMLLMDVRRRELPKTGWEGCKDMFLLPGNSTLIVDEVVDAEAFTEELRVTEDVTVTF

Target ORF information:

RefSeq Version	XM_811651.1
Organism	Trypanosoma cruzi strain CL Brener
Definition	Trypanosoma cruzi strain CL Brener hypothetical protein Tc00.1047053511821.130 partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_811651.1

ORF Insert Sequence:

ATGGCAATAC CGAACCCCCG TCGCTGGAGG CGGGAGTTTG GCGGGACACG AAAGCGAAGA 
ATACGAATAG TGCTTCGTTT TCTTTTCTTT TCATTGTGTC TCTTAGTTAT CGGCGTGTTC 
CTCCTGTTGC TCCTCGCTGC GAACGAGAAG AGGACTTTTG AGAAGGAACT GACCGAGAGG 
GAGATCCCAC ACTATTCTAC GAATGAATTT TTTACGCGTG TCAGCTTCGT CATGAGTAGC 
GCCATGGCCA CGGCGTGGCG CACCTCCAGC GATCGGCTTG AATTCCCAGC GGAGATGTGG 
GTGGAGCGCG TGGCTGTTCG CCCTTCCGCT GCCACGACGG TGCCACTTCC TGCCGTGCTG 
CAGAAGGCAA CGGTGTCGGC CACACTGTAT GGGAAAAGCT CCCTGAAGCT CCAGCGCACC 
AAACGGCGAT CGTTGTACAT TCGCAGTGAC TCACCCATTT TTTTTGTGAC GCCTAACCCT 
GCATTGTCAC CCAACCCATT CAAACGAAAT ACGACGGTGG CGTTGAAGGA ATTTATATTG 
CTTTCCCTAT GGGAGGACAT CCACCGCATC GCCTACCGCA CTTCCGTCGA TCTGCTGCAA 
CATTTAGATT TGATTTTTTC ATATGCGCAA CTGGTGGAAC TACGCTGCTA TCCGCCTTCG 
CAGAAGTCTC ATTTGGATCA CCCATGCGTA CCGTCTGTTG CTGGTGTTTC TGTGCGCGAT 
GGTGTGCATG TGGGGCGGAC CCTTGGTGTT TATTTGGCCA TTGAGCCGCC CGCCCCGGCG 
ATATGGCGGC GAATGAAAGA GGCCTTTTCC GCCTTTGAGG AGTTGGCGAA TGAGTCTCTG 
AGGGAAGTAA TTGAATCCGT GAGTCATGGT TCCCCTGCCG CTCTGCAAAG ACGTGAGTAC 
GAGAGTGTAA TGGAGCACAC CGCACGCATC ACGTATTGGC GGCAAATGTG GCGTGCCGCG 
AAGAAGTCCT CCCCGGGGAA ATTGGAGCAT GCTGTTGAAA TAGGGTCCCG CAACTTTTTG 
AGTTTTAGTG AGCCACGACA TGATATGTCA CATGTGCCGA GTTTTTTGAA AAACAGTTCC 
TTGTTGCCCA GTTATCGGCT TCTGGAAAAG GATCCCTGGC ACTACGGCCT GCACAGTGAG 
ACGGCCATGG GCAAAACGGT TAATCCTAAA AAGCAATTTC TTGATTTACC ACAATACATG 
GCATGGATTG CCATCAACAC GATACTTCAG AATGGTGATT ACGATGATGA GGTGTTTTTT 
TACGGAGTGC TAAAGCCAAT TCAATATGGA AATCCGAAGT TCCAGTTACC GATTTATCTG 
GGTGTGATGG GCTGGGACTA TGACACCGTC TTTTCCCCAT GCCATCGCAA GGTGTGGTTT 
AAGGCATCCG TCATGTATTG TGCTGAAACG ACCCTTGATA AAAGCATTTA TGACTCGGAA 
GCTTTTATTT ATCGATACAC AGATATACTA TGGGATATGA CGCAGCACAT TGACAGGGAG 
CGATTCAGTC GATCTGTTCA AGTGGCGGAG AACGAGCTGC AAACAATACT TTATGGCGCC 
CCAGGGACAA CGATGGCAAC ATTTAATCAG GTAAAACGCG AAAATGACGC TGGCGTGCGG 
TGGATTATTG GGGCCTTTTT GGAACAACGA CAAATATTGC TTCGGGCTTT GGCCTCTTAT 
TTTCCTGTCA ACGAGGTCTC GCATTGCGAT CAAACAAGTG ATAGAGATGC CAAGGCGCTG 
GAGATCCACA ACAGGGAGGC ACATCTCGAA CTGATGAAAC GTCTCTCAAT TGTCGTCAAA 
GTCAAGGGAT CGCGTGTCAG CACAAGATGG GGGTTTTTAG TTGGAGGGAT ACAGTCGGCG 
TTGGGTGTCG ATCTTCCAGA AGATGAGGAG TATCAACTCC CTGATGCTGG TATTTACTGT 
GCACGCGTGT CTGCTGTGGC ATCTTTCTTC ACATCTGTCG GACTGTTGCC TCCTCCCAAA 
GGGCGTGGGA AATGGGAGGC GCCGGGTTTC TGGTTTGTTG ACATGCCATT GGTGGAGCCA 
AGCACGTCGC GTGGCAGTGC AGAGTTTGGG CTGTGGTCCT TTGATAAGAA ATTACCCATG 
CTGTACCCAA TGACAACACC TGATGCAGGG GCCACGCTTA GCATCGTTAT GCCACCTGTG 
TGGCCTCAAG ACTCCAAGGA GAAGCGTGGT GTGTTTGTTT CTCGGGTACT TTTTCCAGAG 
CAGGGTTCAG AAAACTCCCT CTTTAGATGG TCGAGGAAAA ACACGCTTAT CTTCACCATT 
GATGCCCCAA CGCAGAGGAC GATGATTGTA CGTGGCTTCA GTGCTGTACG TTTTTTTGTG 
CCAGAGAAAT CTGAGGAAGA TTCCTTTTCT GCCCAGCGTC ATGAGGGGGA AAATACAGTT 
AGGGGCCCAG GGTTGTTTGC TTCTAACAGC TCCACCACGC GGCTGCGTAT AGGTGATGTG 
GACATTGAAC AGTCGGTACC TCTCGCGTCT GTTGCGGCTC CACCACAAGC GTTATATTTG 
GCCGACGGCC TCGTGGACGC GAAGGGCGTC AGGTTTGGCG GTTCGCTTGA GAAACAGACG 
GTGCTGCGTT GCATCCCTGA AAATGCGTCG GTGGCGGAAT CGGTGGTGGG AGAGGGTTTG 
ACGCTGCGCT CGGCAGAGTG CCCCATTTTT ATTTCCGGCT CTTTTGAAAT TTCTCGAGGA 
GCGACTCTCG ATGTGGCGGC GGGTTGTGTC GTTGTGATGC GGCCTGCTGC GATGCTGCGC 
GTGACGGGGT TTCTGCGGTT CAACGGCACC GTCGCGTCGC CCATTGTGGT CACCAGCGAA 
TCGGCGGAGA ATGCGAGCAG TTGGCAAACC ATCCACGTCG ATGGCCCCAC CGCCACGTTA 
TACGCGAGTT TTACGTTTTT CACCGGCTCA GGCGTGAAGG GGATCCGGGT GGCAAACACT 
GGGAAACACC ACTCCCATTC TGCCGCCATC TCAGCTACAA ATAAGGCCTC CGTCTATCTC 
CGTCACAGTT TCTTGTTGGA GCTGCAGGGG GCAGGCATTG CGGCAGGCAA TGAGGCCGAG 
GTGCAAGTGG AGGATTCATT GGTGCAGTGG GCTCAAATGG GGATCGAGTG TGTGCAGTGC 
ACCTTCACTT CAAGGCGCTC TGTCTGGACG CACTTTCCCT CATGGGATGC AGCGTATGCC 
GATGACGACA ATGACGCCAT GTACCTTTCG GGTGGTAAAC ATCGCGTCAT TGATAGTGTC 
ATTGCCCACA CGAAGGACGA CGGGATTGAC TCTGGTGCGA TGGGTAACGG TGGCTCCCTG 
GTTGTGCACA ACACAATTGT AGAGGGTTGC ATGCATGAGG GGGTGGCTCT CTCCTCCGAT 
CCCAACGGCC GTCGTGAGGT TGTCATTTCT CACACCATCG TGCAGTATTG TCAGCAGGGC 
ATCGAGTCGG GTTATGCGTC GAAACAACAC ACAGCGTCCA TTACGAGTTC TCTCATTCAG 
AAATGCTCCG TCGGTGTTCG TTATGGCGAC AACTACCACT GGTCGCACTG CGACGGGAAA 
CTGACACTGC AGAACACAAC GCTTGCCCAG AACGAAGTGA ATGTGATGAA CTACGAGCGA 
CGGTATCAAA TACCGCAAGA CCCCGAATAC TTCCACTCCG AGGGAGTTTT GTCACAGCGT 
CACTGGAAAC TTCTTCGGGA CGCCGCACGT GATTCCGCTC TCCCGCTGGC GGTGGCGCCA 
CCGGAGGCTG TTGTCATGCT ACTGATGGAT GTGCGACGAA GAGAGCTGCC GAAAACAGGA 
TGGGAGGGCT GCAAGGACAT GTTCTTGCTA CCCGGCAACA GCACTCTTAT CGTTGATGAA 
GTAGTTGATG CGGAGGCGTT TACAGAAGAG CTGAGAGTTA CGGAAGATGT CACAGTCACC 
TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease.
Science (New York, N.Y.)309(5733)409-15(2005 Jul)
El-Sayed NM,Myler PJ,Bartholomeu DC,Nilsson D,Aggarwal G,Tran AN,Ghedin E,Worthey EA,Delcher AL,Blandin G,Westenberger SJ,Caler E,Cerqueira GC,Branche C,Haas B,Anupama A,Arner E,Aslund L,Attipoe P,Bontempi E,Bringaud F,Burton P,Cadag E,Campbell DA,Carrington M,Crabtree J,Darban H,da Silveira JF,de Jong P,Edwards K,Englund PT,Fazelina G,Feldblyum T,Ferella M,Frasch AC,Gull K,Horn D,Hou L,Huang Y,Kindlund E,Klingbeil M,Kluge S,Koo H,Lacerda D,Levin MJ,Lorenzi H,Louie T,Machado CR,McCulloch R,McKenna A,Mizuno Y,Mottram JC,Nelson S,Ochaya S,Osoegawa K,Pai G,Parsons M,Pentony M,Pettersson U,Pop M,Ramirez JL,Rinta J,Robertson L,Salzberg SL,Sanchez DO,Seyler A,Sharma R,Shetty J,Simpson AJ,Sisk E,Tammi MT,Tarleton R,Teixeira S,Van Aken S,Vogt C,Ward PN,Wickstead B,Wortman J,White O,Fraser CM,Stuart KD,Andersson B

Comparative genomics of trypanosomatid parasitic protozoa.
Science (New York, N.Y.)309(5733)404-9(2005 Jul)
El-Sayed NM,Myler PJ,Blandin G,Berriman M,Crabtree J,Aggarwal G,Caler E,Renauld H,Worthey EA,Hertz-Fowler C,Ghedin E,Peacock C,Bartholomeu DC,Haas BJ,Tran AN,Wortman JR,Alsmark UC,Angiuoli S,Anupama A,Badger J,Bringaud F,Cadag E,Carlton JM,Cerqueira GC,Creasy T,Delcher AL,Djikeng A,Embley TM,Hauser C,Ivens AC,Kummerfeld SK,Pereira-Leal JB,Nilsson D,Peterson J,Salzberg SL,Shallom J,Silva JC,Sundaram J,Westenberger S,White O,Melville SE,Donelson JE,Andersson B,Stuart KD,Hall N

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Tc00.1047053511821.130 cDNA ORF clone, Trypanosoma cruzi strain CL Brener

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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