LOC101076483 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101076483
Entrez Gene ID	101076483
Full Name	IQ motif and SEC7 domain-containing protein 2-like
Gene Type	protein-coding
Organism	Takifugu rubripes(torafugu)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011602739.1	XP_011601041.1	IQ motif and SEC7 domain-containing protein 2-like isoform X1
XM_011602739.2	XP_011601041.2	IQ motif and SEC7 domain-containing protein 2-like isoform X1
XM_011602740.1	XP_011601042.1	IQ motif and SEC7 domain-containing protein 2-like isoform X2
XM_029833497.1	XP_029689357.1	IQ motif and SEC7 domain-containing protein 2-like isoform X2
XM_029833498.1	XP_029689358.1	IQ motif and SEC7 domain-containing protein 2-like isoform X3

Pathways from BioSystems
Gene Ontology

The following LOC101076483 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101076483 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTo29683	XM_011602740.1 Latest version!	Takifugu rubripes IQ motif and SEC7 domain-containing protein 2-like (LOC101076483), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OTo29682	XM_011602739.1 Latest version!	Takifugu rubripes IQ motif and SEC7 domain-containing protein 2-like (LOC101076483), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OTo63423	XM_011602739.2 Latest version!	Takifugu rubripes IQ motif and SEC7 domain-containing protein 2-like (LOC101076483), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OTo63424	XM_029833497.1 Latest version!	Takifugu rubripes IQ motif and SEC7 domain-containing protein 2-like (LOC101076483), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OTo63425	XM_029833498.1 Latest version!	Takifugu rubripes IQ motif and SEC7 domain-containing protein 2-like (LOC101076483), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101076483 ( XM_011602740.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011601042.1 Takifugu rubripes IQ motif and SEC7 domain-containing protein 2-like (LOC101076483), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OTo29683
Clone ID Related Accession (Same CDS sequence)	XM_011602740.1
Accession Version	XM_011602740.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426348800000
Organism	Takifugu rubripes(Fugu rubripes)
Product	IQ motif and SEC7 domain-containing protein 2-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070936.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAACA GCACAGAAAG CCCCAACAGG GCGGTGGAGT ATCTCCTGGA GCTCAACAAC 
ATCATCGAGA ACCAGGCCAA GCTCCTGGAG ACCCAGCGCA GACGCATCGA GGAGCTGGAG 
GGCCAGCTGG ACCGCGTCAG CCAGGAGAAC CAGGACCTGA AGCAGGAGCG GAGCCCGCGC 
ACGCCCGGCT CGGACACACC GGAGCAGGAC CACAACCACA GCCAGCCTCT GTCGCTCCCC 
ATCCATCACG GCGGCGGCGC CTCGGCGGCG CAGGTGACGG TCGGCTCGGC CACGACTCCG 
GGGCCCAGCA GGGAGAGGAG GAGCCACACC AGGCTGACCC GGGGGCTCTC CTGCGGCTCG 
TCCACCACCG ACCGAGACCG GCTTGAGCGC ACCGACAGCA CCGACACCAA CAACAGCGCC 
ACCATCCGCC GAAATGTGGA AGGGGAGTCC CAGTTCCCAG ATGTGGGCGC CCGCAAAGAC 
AGCTCCTTCA GCAAGACCCT TTACCGCTCC GAACGCTACG GCGACAGCAG CAGCAGCAGC 
GCACCCTCGG GGTCCAGGGA CCCCTCGTGC GTGGCCCCCT CGAATTCAGC CAGCTTGGCC 
TGGGCGCTCC GAACACGGCA CCAGCCGGCG AGCCTTGCCC TCCGTAAGCA AGAGGAAGAG 
GAGCACAAGA GGTGCAAGGC TCTCTCAGAC AGCTATGAGC TGTCAACAGA TCTGCAGGAT 
AAGAAGGTGG AGATGCTGGA GAGAAAGTAC GGCGGCTCCT TCGTAAGCCG CAGGGCAGCT 
CGAACCATCC AGACGGCGTT CAGGCAGTAC CGCATGAACA AGAACTTTGA ACGCCTCAGG 
AGTTCCGCTT CGGAGAGCCG CATGACTCGG CGCATCATCC TGTCCAACAT GCGACTGCAG 
TACTCCTTTG ACGAGCGGCA GCCGCCACAG CAGGCCCAGA CGCAGACCAA CTTCACCCAC 
AGCGTGGCCA TAGGACCGCC TCACTCCCCC GACACGGACC GCCCGGGAGA CTACACCCAG 
TTAGAGGATT CCTTCTCTAA ACAGGTCAAG TCTTTAGCCG ACTCCATAGA CGATGCTCTC 
ACCTGCCGTG GGGGTCGTAA CGACTCCCAG GAGGGCAGCA GGGAGGGTGG CGGGATTAGC 
GAGGACTTCG GGGAGTGTGT GTGGAGCAGC AGCAGCAACC CCTCCTCCCA AAGAGGATTG 
GGAGAACGCG CCAGGGGGGC AGGAGGAGGA CTCAGCATGC ACGGCGACAG CACCGCCACC 
TCGTACAGCG ACGTCACCCT GTACATGGAT GACGGCATGC CTTCATCGCC CCTGTCCCTG 
GACCGAGCGC CCAGCAGCAC AGACACAGAG TACTGGGGCC CGGGCGGGGG TGTCGGAGGG 
CGCGAGGACA GCAGGGACAC CGAAGGAGGG GGCAGCAGCA ACAGTCGGCG CAGCACCCCG 
TGCACCGAGT GCAGAGACTT CCGTTTTAGA GGCGCCCATC TGCCTCTCCT CACCATCGAG 
CCCCCGAGTG ACAGCTCGGT AGACATGAGC GACCGCTCGG ATCGGGGTTC TTTGAGCAGA 
CAGCTGGTCT ATGAGCAGGA GCCGGGGGTC GGGGTGGGCT CCCCTCAGGG AACCCTCAAA 
CACTCCACCA ACACGGCCAC CCGTGCTTCC TCCACAGCTG CTGCCCAGGG TCAGACTCGG 
GCCCCCGGCA GACCCTTACC CACGCACATC CCGCACCAGG TGGCGGCTCA CCACCACCAT 
CACCACCACC CGGTCCACCA CCATCAGTAC CCCGACACGC CCTCCTCGTC CTCATCGCCC 
CAGCAGCCCC CCACCACCCC TCTGTCCTCC TCCTCGTCCA CCGCTCTGCC TCCCGGTGGG 
CTGGAGCAGC CGTGCTGCTC GGACGGAGAC AACGACTCGC TCAACTCCAC CACTAACTCC 
AACGAGACGG TGAACTGCAG CTCCGGTTCC TCCTCCCAGG ACAGCCTGCA GGAGCCTCTG 
CCACCTCTGG GGAAGCAGAC GTATCAGAGG GAAACGCGCC ACAGCTGGGA CTCGCCACCC 
TTCAACAGCG ATGTGGTGCA GAGACGCCAG TACCGCATAG GCCTTAACCT CTTCAACAAG 
AAGCCAGAAA AGGGAATCCA GTACTTGATA GAGAGGGGAT TCGTATCAGA CACTCCAGTT 
GGAATTGCTC GTTTCATCCT GGAGAGGAAG GGCCTGAGCC GCCAGATGAT TGGAGAGTTC 
CTGGGAAACC GACAGAAACA GTTCAACAAG GATGTTTTGG ACTGTGTGGT GGATGAAATG 
GACTTCTCAG GTATGGACCT GGATGATGCT CTTCGAAAAT TCCAGGCCCA GATTAAGGTT 
CAAGGAGAAG CCCAGAAAGT GGAGCGGCTC ATAGAGGCTT TTAGTCAGAG ATACTGCGTT 
TGTAATCCGG CTCTGGTTCG CCAGTTCCAA AACCCAGATA CCATCTTCAT CCTGGCCTTT 
GCCATAATCC TGCTGAACAC AGACATGTAC AGTCCCAACG TCAAAGCCGA GCGGAAGATG 
AAACTAGAAG ATTTTATAAA GAATTTAAGG GGTGTGGATA ATGGTCAGGA CATACCCAGG 
GACCTGTTGG TCGGGATCTA CCAGAGAATA CAGAAGTGGG AGCTGCGGAC CAACGACGAT 
CACGTTTCAC AAGTGCAGGC AGTCGAGAGA GTCATTGTGG GCAAAAAGCC TGTGCTGTCC 
CTTCCCCATC GCCGGCTGGT GTGTTGCTGC CAGCTTTACG AAGTTCCGGA CCCCAACCGA 
CCTCAAAGGA CGGGGGTGCA CCAACGGGAG GTCTTTCTCT TTAACGACCT TCTGGTGGTG 
ACCAAAATCT TCCAGAAGAA GAAAACTTCT GTCACCTACA GTTTCAGACA GTCCTTCTCT 
TTGGTTGAGA TGCAAGTGCA AATGTTCCAA AACTCATACT ACCCTCATGG TATTCGTCTG 
ACCTCGGCGA TCCTGGGCGG CGAGAGGAAG GTTCTCATCG TCTTTATGGC GCCCAGTCAA 
CAAGACCGCA CTCGCTTCGT CAGTGACCTC AGGGAGAGTA TTGCTGAAGT TCAAGAGATG 
GAGAAATATA GGGTTGAATC GGAGCTGGAA AAGCAAAAAG GTGTGATGCG GCCCAGTTTG 
CTGACTGGAG GCGTGGTAGG AGGAGGCGTG GGTGTGAAGG GCGACGTGGT GAACGGCACC 
CTGGGAAGGA CGAGTTTTGA CGACAACTGT GAGGGGCTGA AACGCACCGC GCTCAGCTCC 
TCTCTCAGGG ACCTCTCGGA CACGGGGCTC CATCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011601042.1
CDS	205..3483
Translation	MENSTESPNRAVEYLLELNNIIENQAKLLETQRRRIEELEGQLDRVSQENQDLKQERSPRTPGSDTPEQDHNHSQPLSLPIHHGGGASAAQVTVGSATTPGPSRERRSHTRLTRGLSCGSSTTDRDRLERTDSTDTNNSATIRRNVEGESQFPDVGARKDSSFSKTLYRSERYGDSSSSSAPSGSRDPSCVAPSNSASLAWALRTRHQPASLALRKQEEEEHKRCKALSDSYELSTDLQDKKVEMLERKYGGSFVSRRAARTIQTAFRQYRMNKNFERLRSSASESRMTRRIILSNMRLQYSFDERQPPQQAQTQTNFTHSVAIGPPHSPDTDRPGDYTQLEDSFSKQVKSLADSIDDALTCRGGRNDSQEGSREGGGISEDFGECVWSSSSNPSSQRGLGERARGAGGGLSMHGDSTATSYSDVTLYMDDGMPSSPLSLDRAPSSTDTEYWGPGGGVGGREDSRDTEGGGSSNSRRSTPCTECRDFRFRGAHLPLLTIEPPSDSSVDMSDRSDRGSLSRQLVYEQEPGVGVGSPQGTLKHSTNTATRASSTAAAQGQTRAPGRPLPTHIPHQVAAHHHHHHHPVHHHQYPDTPSSSSSPQQPPTTPLSSSSSTALPPGGLEQPCCSDGDNDSLNSTTNSNETVNCSSGSSSQDSLQEPLPPLGKQTYQRETRHSWDSPPFNSDVVQRRQYRIGLNLFNKKPEKGIQYLIERGFVSDTPVGIARFILERKGLSRQMIGEFLGNRQKQFNKDVLDCVVDEMDFSGMDLDDALRKFQAQIKVQGEAQKVERLIEAFSQRYCVCNPALVRQFQNPDTIFILAFAIILLNTDMYSPNVKAERKMKLEDFIKNLRGVDNGQDIPRDLLVGIYQRIQKWELRTNDDHVSQVQAVERVIVGKKPVLSLPHRRLVCCCQLYEVPDPNRPQRTGVHQREVFLFNDLLVVTKIFQKKKTSVTYSFRQSFSLVEMQVQMFQNSYYPHGIRLTSAILGGERKVLIVFMAPSQQDRTRFVSDLRESIAEVQEMEKYRVESELEKQKGVMRPSLLTGGVVGGGVGVKGDVVNGTLGRTSFDDNCEGLKRTALSSSLRDLSDTGLHH

Target ORF information:

RefSeq Version	XM_011602740.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes IQ motif and SEC7 domain-containing protein 2-like (LOC101076483), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011602740.1

ORF Insert Sequence:

ATGGAGAACA GCACAGAAAG CCCCAACAGG GCGGTGGAGT ATCTCCTGGA GCTCAACAAC 
ATCATCGAGA ACCAGGCCAA GCTCCTGGAG ACCCAGCGCA GACGCATCGA GGAGCTGGAG 
GGCCAGCTGG ACCGCGTCAG CCAGGAGAAC CAGGACCTGA AGCAGGAGCG GAGCCCGCGC 
ACGCCCGGCT CGGACACACC GGAGCAGGAC CACAACCACA GCCAGCCTCT GTCGCTCCCC 
ATCCATCACG GCGGCGGCGC CTCGGCGGCG CAGGTGACGG TCGGCTCGGC CACGACTCCG 
GGGCCCAGCA GGGAGAGGAG GAGCCACACC AGGCTGACCC GGGGGCTCTC CTGCGGCTCG 
TCCACCACCG ACCGAGACCG GCTTGAGCGC ACCGACAGCA CCGACACCAA CAACAGCGCC 
ACCATCCGCC GAAATGTGGA AGGGGAGTCC CAGTTCCCAG ATGTGGGCGC CCGCAAAGAC 
AGCTCCTTCA GCAAGACCCT TTACCGCTCC GAACGCTACG GCGACAGCAG CAGCAGCAGC 
GCACCCTCGG GGTCCAGGGA CCCCTCGTGC GTGGCCCCCT CGAATTCAGC CAGCTTGGCC 
TGGGCGCTCC GAACACGGCA CCAGCCGGCG AGCCTTGCCC TCCGTAAGCA AGAGGAAGAG 
GAGCACAAGA GGTGCAAGGC TCTCTCAGAC AGCTATGAGC TGTCAACAGA TCTGCAGGAT 
AAGAAGGTGG AGATGCTGGA GAGAAAGTAC GGCGGCTCCT TCGTAAGCCG CAGGGCAGCT 
CGAACCATCC AGACGGCGTT CAGGCAGTAC CGCATGAACA AGAACTTTGA ACGCCTCAGG 
AGTTCCGCTT CGGAGAGCCG CATGACTCGG CGCATCATCC TGTCCAACAT GCGACTGCAG 
TACTCCTTTG ACGAGCGGCA GCCGCCACAG CAGGCCCAGA CGCAGACCAA CTTCACCCAC 
AGCGTGGCCA TAGGACCGCC TCACTCCCCC GACACGGACC GCCCGGGAGA CTACACCCAG 
TTAGAGGATT CCTTCTCTAA ACAGGTCAAG TCTTTAGCCG ACTCCATAGA CGATGCTCTC 
ACCTGCCGTG GGGGTCGTAA CGACTCCCAG GAGGGCAGCA GGGAGGGTGG CGGGATTAGC 
GAGGACTTCG GGGAGTGTGT GTGGAGCAGC AGCAGCAACC CCTCCTCCCA AAGAGGATTG 
GGAGAACGCG CCAGGGGGGC AGGAGGAGGA CTCAGCATGC ACGGCGACAG CACCGCCACC 
TCGTACAGCG ACGTCACCCT GTACATGGAT GACGGCATGC CTTCATCGCC CCTGTCCCTG 
GACCGAGCGC CCAGCAGCAC AGACACAGAG TACTGGGGCC CGGGCGGGGG TGTCGGAGGG 
CGCGAGGACA GCAGGGACAC CGAAGGAGGG GGCAGCAGCA ACAGTCGGCG CAGCACCCCG 
TGCACCGAGT GCAGAGACTT CCGTTTTAGA GGCGCCCATC TGCCTCTCCT CACCATCGAG 
CCCCCGAGTG ACAGCTCGGT AGACATGAGC GACCGCTCGG ATCGGGGTTC TTTGAGCAGA 
CAGCTGGTCT ATGAGCAGGA GCCGGGGGTC GGGGTGGGCT CCCCTCAGGG AACCCTCAAA 
CACTCCACCA ACACGGCCAC CCGTGCTTCC TCCACAGCTG CTGCCCAGGG TCAGACTCGG 
GCCCCCGGCA GACCCTTACC CACGCACATC CCGCACCAGG TGGCGGCTCA CCACCACCAT 
CACCACCACC CGGTCCACCA CCATCAGTAC CCCGACACGC CCTCCTCGTC CTCATCGCCC 
CAGCAGCCCC CCACCACCCC TCTGTCCTCC TCCTCGTCCA CCGCTCTGCC TCCCGGTGGG 
CTGGAGCAGC CGTGCTGCTC GGACGGAGAC AACGACTCGC TCAACTCCAC CACTAACTCC 
AACGAGACGG TGAACTGCAG CTCCGGTTCC TCCTCCCAGG ACAGCCTGCA GGAGCCTCTG 
CCACCTCTGG GGAAGCAGAC GTATCAGAGG GAAACGCGCC ACAGCTGGGA CTCGCCACCC 
TTCAACAGCG ATGTGGTGCA GAGACGCCAG TACCGCATAG GCCTTAACCT CTTCAACAAG 
AAGCCAGAAA AGGGAATCCA GTACTTGATA GAGAGGGGAT TCGTATCAGA CACTCCAGTT 
GGAATTGCTC GTTTCATCCT GGAGAGGAAG GGCCTGAGCC GCCAGATGAT TGGAGAGTTC 
CTGGGAAACC GACAGAAACA GTTCAACAAG GATGTTTTGG ACTGTGTGGT GGATGAAATG 
GACTTCTCAG GTATGGACCT GGATGATGCT CTTCGAAAAT TCCAGGCCCA GATTAAGGTT 
CAAGGAGAAG CCCAGAAAGT GGAGCGGCTC ATAGAGGCTT TTAGTCAGAG ATACTGCGTT 
TGTAATCCGG CTCTGGTTCG CCAGTTCCAA AACCCAGATA CCATCTTCAT CCTGGCCTTT 
GCCATAATCC TGCTGAACAC AGACATGTAC AGTCCCAACG TCAAAGCCGA GCGGAAGATG 
AAACTAGAAG ATTTTATAAA GAATTTAAGG GGTGTGGATA ATGGTCAGGA CATACCCAGG 
GACCTGTTGG TCGGGATCTA CCAGAGAATA CAGAAGTGGG AGCTGCGGAC CAACGACGAT 
CACGTTTCAC AAGTGCAGGC AGTCGAGAGA GTCATTGTGG GCAAAAAGCC TGTGCTGTCC 
CTTCCCCATC GCCGGCTGGT GTGTTGCTGC CAGCTTTACG AAGTTCCGGA CCCCAACCGA 
CCTCAAAGGA CGGGGGTGCA CCAACGGGAG GTCTTTCTCT TTAACGACCT TCTGGTGGTG 
ACCAAAATCT TCCAGAAGAA GAAAACTTCT GTCACCTACA GTTTCAGACA GTCCTTCTCT 
TTGGTTGAGA TGCAAGTGCA AATGTTCCAA AACTCATACT ACCCTCATGG TATTCGTCTG 
ACCTCGGCGA TCCTGGGCGG CGAGAGGAAG GTTCTCATCG TCTTTATGGC GCCCAGTCAA 
CAAGACCGCA CTCGCTTCGT CAGTGACCTC AGGGAGAGTA TTGCTGAAGT TCAAGAGATG 
GAGAAATATA GGGTTGAATC GGAGCTGGAA AAGCAAAAAG GTGTGATGCG GCCCAGTTTG 
CTGACTGGAG GCGTGGTAGG AGGAGGCGTG GGTGTGAAGG GCGACGTGGT GAACGGCACC 
CTGGGAAGGA CGAGTTTTGA CGACAACTGT GAGGGGCTGA AACGCACCGC GCTCAGCTCC 
TCTCTCAGGG ACCTCTCGGA CACGGGGCTC CATCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101076483 ( XM_011602739.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011601041.1 Takifugu rubripes IQ motif and SEC7 domain-containing protein 2-like (LOC101076483), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTo29682
Clone ID Related Accession (Same CDS sequence)	XM_011602739.1
Accession Version	XM_011602739.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4617bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426348800000
Organism	Takifugu rubripes(Fugu rubripes)
Product	IQ motif and SEC7 domain-containing protein 2-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070936.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAACA GCACAGAAAG CCCCAACAGG GCGGTGGAGT ATCTCCTGGA GCTCAACAAC 
ATCATCGAGA ACCAGGCCAA GCTCCTGGAG ACCCAGCGCA GACGCATCGA GGAGCTGGAG 
GGCCAGCTGG ACCGCGTCAG CCAGGAGAAC CAGGACCTGA AGCAGGAGCG GAGCCCGCGC 
ACGCCCGGCT CGGACACACC GGAGCAGGAC CACAACCACA GCCAGCCTCT GTCGCTCCCC 
ATCCATCACG GCGGCGGCGC CTCGGCGGCG CAGGTGACGG TCGGCTCGGC CACGACTCCG 
GGGCCCAGCA GGGAGAGGAG GAGCCACACC AGGCTGACCC GGGGGCTCTC CTGCGGCTCG 
TCCACCACCG ACCGAGACCG GCTTGAGCGC ACCGACAGCA CCGACACCAA CAACAGCGCC 
ACCATCCGCC GAAATGTGGA AGGGGAGTCC CAGTTCCCAG ATGTGGGCGC CCGCAAAGAC 
AGCTCCTTCA GCAAGACCCT TTACCGCTCC GAACGCTACG GCGACAGCAG CAGCAGCAGC 
GCACCCTCGG GGTCCAGGGA CCCCTCGTGC GTGGCCCCCT CGAATTCAGC CAGCTTGGCC 
TGGGCGCTCC GAACACGGCA CCAGCCGGCG AGCCTTGCCC TCCGTAAGCA AGAGGAAGAG 
GAGCACAAGA GGTGCAAGGC TCTCTCAGAC AGCTATGAGC TGTCAACAGA TCTGCAGGAT 
AAGAAGGTGG AGATGCTGGA GAGAAAGTAC GGCGGCTCCT TCGTAAGCCG CAGGGCAGCT 
CGAACCATCC AGACGGCGTT CAGGCAGTAC CGCATGAACA AGAACTTTGA ACGCCTCAGG 
AGTTCCGCTT CGGAGAGCCG CATGACTCGG CGCATCATCC TGTCCAACAT GCGACTGCAG 
TACTCCTTTG ACGAGCGGCA GCCGCCACAG CAGGCCCAGA CGCAGACCAA CTTCACCCAC 
AGCGTGGCCA TAGGACCGCC TCACTCCCCC GACACGGACC GCCCGGGAGA CTACACCCAG 
TTAGAGGATT CCTTCTCTAA ACAGGTCAAG TCTTTAGCCG ACTCCATAGA CGATGCTCTC 
ACCTGCCGTG GGGGTCGTAA CGACTCCCAG GAGGGCAGCA GGGAGGGTGG CGGGATTAGC 
GAGGACTTCG GGGAGTGTGT GTGGAGCAGC AGCAGCAACC CCTCCTCCCA AAGAGGATTG 
GGAGAACGCG CCAGGGGGGC AGGAGGAGGA CTCAGCATGC ACGGCGACAG CACCGCCACC 
TCGTACAGCG ACGTCACCCT GTACATGGAT GACGGCATGC CTTCATCGCC CCTGTCCCTG 
GACCGAGCGC CCAGCAGCAC AGACACAGAG TACTGGGGCC CGGGCGGGGG TGTCGGAGGG 
CGCGAGGACA GCAGGGACAC CGAAGGAGGG GGCAGCAGCA ACAGTCGGCG CAGCACCCCG 
TGCACCGAGT GCAGAGACTT CCGTTTTAGA GGCGCCCATC TGCCTCTCCT CACCATCGAG 
CCCCCGAGTG ACAGCTCGGT AGACATGAGC GACCGCTCGG ATCGGGGTTC TTTGAGCAGA 
CAGCTGGTCT ATGAGCAGGA GCCGGGGGTC GGGGTGGGCT CCCCTCAGGG AACCCTCAAA 
CACTCCACCA ACACGGCCAC CCGTGCTTCC TCCACAGCTG CTGCCCAGGG TCAGACTCGG 
GCCCCCGGCA GACCCTTACC CACGCACATC CCGCACCAGG TGGCGGCTCA CCACCACCAT 
CACCACCACC CGGTCCACCA CCATCAGTAC CCCGACACGC CCTCCTCGTC CTCATCGCCC 
CAGCAGCCCC CCACCACCCC TCTGTCCTCC TCCTCGTCCA CCGCTCTGCC TCCCGGTGGG 
CTGGAGCAGC CGTGCTGCTC GGACGGAGAC AACGACTCGC TCAACTCCAC CACTAACTCC 
AACGAGACGG TGAACTGCAG CTCCGGTTCC TCCTCCCAGG ACAGCCTGCA GGAGCCTCTG 
CCACCTCTGG GGAAGCAGAC GTATCAGAGG GAAACGCGCC ACAGCTGGGA CTCGCCACCC 
TTCAACAGCG ATGTGGTGCA GAGACGCCAG TACCGCATAG GCCTTAACCT CTTCAACAAG 
AAGCCAGAAA AGGGAATCCA GTACTTGATA GAGAGGGGAT TCGTATCAGA CACTCCAGTT 
GGAATTGCTC GTTTCATCCT GGAGAGGAAG GGCCTGAGCC GCCAGATGAT TGGAGAGTTC 
CTGGGAAACC GACAGAAACA GTTCAACAAG GATGTTTTGG ACTGTGTGGT GGATGAAATG 
GACTTCTCAG GTATGGACCT GGATGATGCT CTTCGAAAAT TCCAGGCCCA GATTAAGGTT 
CAAGGAGAAG CCCAGAAAGT GGAGCGGCTC ATAGAGGCTT TTAGTCAGAG ATACTGCGTT 
TGTAATCCGG CTCTGGTTCG CCAGTTCCAA AACCCAGATA CCATCTTCAT CCTGGCCTTT 
GCCATAATCC TGCTGAACAC AGACATGTAC AGTCCCAACG TCAAAGCCGA GCGGAAGATG 
AAACTAGAAG ATTTTATAAA GAATTTAAGG GGTGTGGATA ATGGTCAGGA CATACCCAGG 
GACCTGTTGG TCGGGATCTA CCAGAGAATA CAGAAGTGGG AGCTGCGGAC CAACGACGAT 
CACGTTTCAC AAGTGCAGGC AGTCGAGAGA GTCATTGTGG GCAAAAAGCC TGTGCTGTCC 
CTTCCCCATC GCCGGCTGGT GTGTTGCTGC CAGCTTTACG AAGTTCCGGA CCCCAACCGA 
CCTCAAAGGA CGGGGGTGCA CCAACGGGAG GTCTTTCTCT TTAACGACCT TCTGGTGGTG 
ACCAAAATCT TCCAGAAGAA GAAAACTTCT GTCACCTACA GTTTCAGACA GTCCTTCTCT 
TTGGTTGAGA TGCAAGTGCA AATGTTCCAA AACTCATACT ACCCTCATGG TATTCGTCTG 
ACCTCGGCGA TCCTGGGCGG CGAGAGGAAG GTTCTCATCG TCTTTATGGC GCCCAGTCAA 
CAAGACCGCA CTCGCTTCGT CAGTGACCTC AGGGAGAGTA TTGCTGAAGT TCAAGAGATG 
GAGAAATATA GGGTTGAATC GGAGCTGGAA AAGCAAAAAG GTGTGATGCG GCCCAGTTTG 
CTGACTGGAG GCGTGGTAGG AGGAGGCGTG GGTGTGAAGG GCGACGTGGT GAACGGCACC 
CTGGGAAGGA CGAGTTTTGA CGACAACTGT GAGGGGCTGA AACGCACCGC GCTCAGCTCC 
TCTCTCAGGG ACCTCTCGGA CACGGGGAAA CATGGTCGCA GGAACAGTGT TGGTTCTCTG 
GATAGTACCA TGGAAGGCTC CATCATTAGC AGTCCCCAGC CTCACCAGCG ATACCCACTG 
CCGGGAGTGA TGCCGGGTGT GATGCCAGGT GTGCTGTCGG GTGTGATGCC GGGTGTGATG 
CCGGGTGTGA TGCCGGGTGT GATGCCGGGT GTGATGCCAG GTGTGATGCC GGGTGTTCTC 
CCAGGTGTGA TCCCGGGATG CTATGGAACA GATGACTTCC GGTCTCATCG CCCCATCCTG 
AGCCCCGGGA CGGGGGTGGG GGGTGGGCCG GGGCAGCAAA ATACCACTGC TGGGACAGAA 
GTTGGGGGAG TCTCCAGCAG TGTAGAGAGA GCAGGAGCGG GGAGCAACCC TGGGGGGAGC 
AGCAGCAACA ACAGCAGCTC TTTTCTGGGA TCCCTGTTTG GCAGCAAACG TGCCAAACCG 
CCAGGGCCCC TTATTCCACC GGGGCCACCC TTCCCCGCAC CTGTCCCCCC GCCGACTTCG 
GGAGGGCCCC CTCCTCACTC CCCTTCATCT CTCTGTCAGA TGGACGGGGG ACCTTCCAAG 
ATCCAAGCGC TGCATGCGCA GTACTGTCAC ACGGGCGCCG TGCAGCCTCC ACCACCGTAC 
TACCATCACC ATCGCTTCCA CGTCCAGCCT GTCCAGGCCC CTTTGCAAGG CCTACCCCCC 
CATACTATAC AGAGAGGCCC GCTACCCTGT CGGCCAGTGC TTGGCCCCCC ACACACCCAG 
CACCTGCAGC TGGCCCATCT CTCCCAGCTC TCCCAGCCTG GCCTTCCGGG TCGCTACGGC 
AACCTGGTGT TGGGATGTCC TCCCCCTCTT TCTCCTCTCT CCCAACATTC CCAGAACCCC 
CAGTGCGCCC TCTACCACGT TCAGCCCACA CACTCTATCA GAGGGGGCGG CGGTCCTGGT 
ACCAAACTAC CACTAACCGT GTCACACTCC CACCCACACC CCCACGCACA CTCCCAAACC 
CACCCCGGAC ACGTGCACAC GCCCCACCCA GCCAGCCAAT CCACGCACCA AACACACTTT 
ATATTCGGCA CTCTGCCCCA CAACCTTCCG TCCGCCTCCG TCAATGCGCA TCACGCAGCC 
TCCGCCGCCC CGTATCTGCC CCAGTACCCT CCTCTCTCCT CCATCCCACC TCCCCCACCG 
CACTCCCCTT TACCCCCCCC TTCGTCCCCC CTTACCCCTC TTACCCCTCT CTCCCCTCAC 
CCCCCCCAGC ACCCCGGGCA GCCTCAGCAG GGGCCACAGG TGACTGGGGC TGGAACGACA 
GGTACAGGGG GCAGCTCCAA ATCCAAGCCT ATCAACCGGA TAAGCACCGT GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011601041.1
CDS	205..4821
Translation	MENSTESPNRAVEYLLELNNIIENQAKLLETQRRRIEELEGQLDRVSQENQDLKQERSPRTPGSDTPEQDHNHSQPLSLPIHHGGGASAAQVTVGSATTPGPSRERRSHTRLTRGLSCGSSTTDRDRLERTDSTDTNNSATIRRNVEGESQFPDVGARKDSSFSKTLYRSERYGDSSSSSAPSGSRDPSCVAPSNSASLAWALRTRHQPASLALRKQEEEEHKRCKALSDSYELSTDLQDKKVEMLERKYGGSFVSRRAARTIQTAFRQYRMNKNFERLRSSASESRMTRRIILSNMRLQYSFDERQPPQQAQTQTNFTHSVAIGPPHSPDTDRPGDYTQLEDSFSKQVKSLADSIDDALTCRGGRNDSQEGSREGGGISEDFGECVWSSSSNPSSQRGLGERARGAGGGLSMHGDSTATSYSDVTLYMDDGMPSSPLSLDRAPSSTDTEYWGPGGGVGGREDSRDTEGGGSSNSRRSTPCTECRDFRFRGAHLPLLTIEPPSDSSVDMSDRSDRGSLSRQLVYEQEPGVGVGSPQGTLKHSTNTATRASSTAAAQGQTRAPGRPLPTHIPHQVAAHHHHHHHPVHHHQYPDTPSSSSSPQQPPTTPLSSSSSTALPPGGLEQPCCSDGDNDSLNSTTNSNETVNCSSGSSSQDSLQEPLPPLGKQTYQRETRHSWDSPPFNSDVVQRRQYRIGLNLFNKKPEKGIQYLIERGFVSDTPVGIARFILERKGLSRQMIGEFLGNRQKQFNKDVLDCVVDEMDFSGMDLDDALRKFQAQIKVQGEAQKVERLIEAFSQRYCVCNPALVRQFQNPDTIFILAFAIILLNTDMYSPNVKAERKMKLEDFIKNLRGVDNGQDIPRDLLVGIYQRIQKWELRTNDDHVSQVQAVERVIVGKKPVLSLPHRRLVCCCQLYEVPDPNRPQRTGVHQREVFLFNDLLVVTKIFQKKKTSVTYSFRQSFSLVEMQVQMFQNSYYPHGIRLTSAILGGERKVLIVFMAPSQQDRTRFVSDLRESIAEVQEMEKYRVESELEKQKGVMRPSLLTGGVVGGGVGVKGDVVNGTLGRTSFDDNCEGLKRTALSSSLRDLSDTGKHGRRNSVGSLDSTMEGSIISSPQPHQRYPLPGVMPGVMPGVLSGVMPGVMPGVMPGVMPGVMPGVMPGVLPGVIPGCYGTDDFRSHRPILSPGTGVGGGPGQQNTTAGTEVGGVSSSVERAGAGSNPGGSSSNNSSSFLGSLFGSKRAKPPGPLIPPGPPFPAPVPPPTSGGPPPHSPSSLCQMDGGPSKIQALHAQYCHTGAVQPPPPYYHHHRFHVQPVQAPLQGLPPHTIQRGPLPCRPVLGPPHTQHLQLAHLSQLSQPGLPGRYGNLVLGCPPPLSPLSQHSQNPQCALYHVQPTHSIRGGGGPGTKLPLTVSHSHPHPHAHSQTHPGHVHTPHPASQSTHQTHFIFGTLPHNLPSASVNAHHAASAAPYLPQYPPLSSIPPPPPHSPLPPPSSPLTPLTPLSPHPPQHPGQPQQGPQVTGAGTTGTGGSSKSKPINRISTVV

Target ORF information:

RefSeq Version	XM_011602739.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes IQ motif and SEC7 domain-containing protein 2-like (LOC101076483), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011602739.1

ORF Insert Sequence:

ATGGAGAACA GCACAGAAAG CCCCAACAGG GCGGTGGAGT ATCTCCTGGA GCTCAACAAC 
ATCATCGAGA ACCAGGCCAA GCTCCTGGAG ACCCAGCGCA GACGCATCGA GGAGCTGGAG 
GGCCAGCTGG ACCGCGTCAG CCAGGAGAAC CAGGACCTGA AGCAGGAGCG GAGCCCGCGC 
ACGCCCGGCT CGGACACACC GGAGCAGGAC CACAACCACA GCCAGCCTCT GTCGCTCCCC 
ATCCATCACG GCGGCGGCGC CTCGGCGGCG CAGGTGACGG TCGGCTCGGC CACGACTCCG 
GGGCCCAGCA GGGAGAGGAG GAGCCACACC AGGCTGACCC GGGGGCTCTC CTGCGGCTCG 
TCCACCACCG ACCGAGACCG GCTTGAGCGC ACCGACAGCA CCGACACCAA CAACAGCGCC 
ACCATCCGCC GAAATGTGGA AGGGGAGTCC CAGTTCCCAG ATGTGGGCGC CCGCAAAGAC 
AGCTCCTTCA GCAAGACCCT TTACCGCTCC GAACGCTACG GCGACAGCAG CAGCAGCAGC 
GCACCCTCGG GGTCCAGGGA CCCCTCGTGC GTGGCCCCCT CGAATTCAGC CAGCTTGGCC 
TGGGCGCTCC GAACACGGCA CCAGCCGGCG AGCCTTGCCC TCCGTAAGCA AGAGGAAGAG 
GAGCACAAGA GGTGCAAGGC TCTCTCAGAC AGCTATGAGC TGTCAACAGA TCTGCAGGAT 
AAGAAGGTGG AGATGCTGGA GAGAAAGTAC GGCGGCTCCT TCGTAAGCCG CAGGGCAGCT 
CGAACCATCC AGACGGCGTT CAGGCAGTAC CGCATGAACA AGAACTTTGA ACGCCTCAGG 
AGTTCCGCTT CGGAGAGCCG CATGACTCGG CGCATCATCC TGTCCAACAT GCGACTGCAG 
TACTCCTTTG ACGAGCGGCA GCCGCCACAG CAGGCCCAGA CGCAGACCAA CTTCACCCAC 
AGCGTGGCCA TAGGACCGCC TCACTCCCCC GACACGGACC GCCCGGGAGA CTACACCCAG 
TTAGAGGATT CCTTCTCTAA ACAGGTCAAG TCTTTAGCCG ACTCCATAGA CGATGCTCTC 
ACCTGCCGTG GGGGTCGTAA CGACTCCCAG GAGGGCAGCA GGGAGGGTGG CGGGATTAGC 
GAGGACTTCG GGGAGTGTGT GTGGAGCAGC AGCAGCAACC CCTCCTCCCA AAGAGGATTG 
GGAGAACGCG CCAGGGGGGC AGGAGGAGGA CTCAGCATGC ACGGCGACAG CACCGCCACC 
TCGTACAGCG ACGTCACCCT GTACATGGAT GACGGCATGC CTTCATCGCC CCTGTCCCTG 
GACCGAGCGC CCAGCAGCAC AGACACAGAG TACTGGGGCC CGGGCGGGGG TGTCGGAGGG 
CGCGAGGACA GCAGGGACAC CGAAGGAGGG GGCAGCAGCA ACAGTCGGCG CAGCACCCCG 
TGCACCGAGT GCAGAGACTT CCGTTTTAGA GGCGCCCATC TGCCTCTCCT CACCATCGAG 
CCCCCGAGTG ACAGCTCGGT AGACATGAGC GACCGCTCGG ATCGGGGTTC TTTGAGCAGA 
CAGCTGGTCT ATGAGCAGGA GCCGGGGGTC GGGGTGGGCT CCCCTCAGGG AACCCTCAAA 
CACTCCACCA ACACGGCCAC CCGTGCTTCC TCCACAGCTG CTGCCCAGGG TCAGACTCGG 
GCCCCCGGCA GACCCTTACC CACGCACATC CCGCACCAGG TGGCGGCTCA CCACCACCAT 
CACCACCACC CGGTCCACCA CCATCAGTAC CCCGACACGC CCTCCTCGTC CTCATCGCCC 
CAGCAGCCCC CCACCACCCC TCTGTCCTCC TCCTCGTCCA CCGCTCTGCC TCCCGGTGGG 
CTGGAGCAGC CGTGCTGCTC GGACGGAGAC AACGACTCGC TCAACTCCAC CACTAACTCC 
AACGAGACGG TGAACTGCAG CTCCGGTTCC TCCTCCCAGG ACAGCCTGCA GGAGCCTCTG 
CCACCTCTGG GGAAGCAGAC GTATCAGAGG GAAACGCGCC ACAGCTGGGA CTCGCCACCC 
TTCAACAGCG ATGTGGTGCA GAGACGCCAG TACCGCATAG GCCTTAACCT CTTCAACAAG 
AAGCCAGAAA AGGGAATCCA GTACTTGATA GAGAGGGGAT TCGTATCAGA CACTCCAGTT 
GGAATTGCTC GTTTCATCCT GGAGAGGAAG GGCCTGAGCC GCCAGATGAT TGGAGAGTTC 
CTGGGAAACC GACAGAAACA GTTCAACAAG GATGTTTTGG ACTGTGTGGT GGATGAAATG 
GACTTCTCAG GTATGGACCT GGATGATGCT CTTCGAAAAT TCCAGGCCCA GATTAAGGTT 
CAAGGAGAAG CCCAGAAAGT GGAGCGGCTC ATAGAGGCTT TTAGTCAGAG ATACTGCGTT 
TGTAATCCGG CTCTGGTTCG CCAGTTCCAA AACCCAGATA CCATCTTCAT CCTGGCCTTT 
GCCATAATCC TGCTGAACAC AGACATGTAC AGTCCCAACG TCAAAGCCGA GCGGAAGATG 
AAACTAGAAG ATTTTATAAA GAATTTAAGG GGTGTGGATA ATGGTCAGGA CATACCCAGG 
GACCTGTTGG TCGGGATCTA CCAGAGAATA CAGAAGTGGG AGCTGCGGAC CAACGACGAT 
CACGTTTCAC AAGTGCAGGC AGTCGAGAGA GTCATTGTGG GCAAAAAGCC TGTGCTGTCC 
CTTCCCCATC GCCGGCTGGT GTGTTGCTGC CAGCTTTACG AAGTTCCGGA CCCCAACCGA 
CCTCAAAGGA CGGGGGTGCA CCAACGGGAG GTCTTTCTCT TTAACGACCT TCTGGTGGTG 
ACCAAAATCT TCCAGAAGAA GAAAACTTCT GTCACCTACA GTTTCAGACA GTCCTTCTCT 
TTGGTTGAGA TGCAAGTGCA AATGTTCCAA AACTCATACT ACCCTCATGG TATTCGTCTG 
ACCTCGGCGA TCCTGGGCGG CGAGAGGAAG GTTCTCATCG TCTTTATGGC GCCCAGTCAA 
CAAGACCGCA CTCGCTTCGT CAGTGACCTC AGGGAGAGTA TTGCTGAAGT TCAAGAGATG 
GAGAAATATA GGGTTGAATC GGAGCTGGAA AAGCAAAAAG GTGTGATGCG GCCCAGTTTG 
CTGACTGGAG GCGTGGTAGG AGGAGGCGTG GGTGTGAAGG GCGACGTGGT GAACGGCACC 
CTGGGAAGGA CGAGTTTTGA CGACAACTGT GAGGGGCTGA AACGCACCGC GCTCAGCTCC 
TCTCTCAGGG ACCTCTCGGA CACGGGGAAA CATGGTCGCA GGAACAGTGT TGGTTCTCTG 
GATAGTACCA TGGAAGGCTC CATCATTAGC AGTCCCCAGC CTCACCAGCG ATACCCACTG 
CCGGGAGTGA TGCCGGGTGT GATGCCAGGT GTGCTGTCGG GTGTGATGCC GGGTGTGATG 
CCGGGTGTGA TGCCGGGTGT GATGCCGGGT GTGATGCCAG GTGTGATGCC GGGTGTTCTC 
CCAGGTGTGA TCCCGGGATG CTATGGAACA GATGACTTCC GGTCTCATCG CCCCATCCTG 
AGCCCCGGGA CGGGGGTGGG GGGTGGGCCG GGGCAGCAAA ATACCACTGC TGGGACAGAA 
GTTGGGGGAG TCTCCAGCAG TGTAGAGAGA GCAGGAGCGG GGAGCAACCC TGGGGGGAGC 
AGCAGCAACA ACAGCAGCTC TTTTCTGGGA TCCCTGTTTG GCAGCAAACG TGCCAAACCG 
CCAGGGCCCC TTATTCCACC GGGGCCACCC TTCCCCGCAC CTGTCCCCCC GCCGACTTCG 
GGAGGGCCCC CTCCTCACTC CCCTTCATCT CTCTGTCAGA TGGACGGGGG ACCTTCCAAG 
ATCCAAGCGC TGCATGCGCA GTACTGTCAC ACGGGCGCCG TGCAGCCTCC ACCACCGTAC 
TACCATCACC ATCGCTTCCA CGTCCAGCCT GTCCAGGCCC CTTTGCAAGG CCTACCCCCC 
CATACTATAC AGAGAGGCCC GCTACCCTGT CGGCCAGTGC TTGGCCCCCC ACACACCCAG 
CACCTGCAGC TGGCCCATCT CTCCCAGCTC TCCCAGCCTG GCCTTCCGGG TCGCTACGGC 
AACCTGGTGT TGGGATGTCC TCCCCCTCTT TCTCCTCTCT CCCAACATTC CCAGAACCCC 
CAGTGCGCCC TCTACCACGT TCAGCCCACA CACTCTATCA GAGGGGGCGG CGGTCCTGGT 
ACCAAACTAC CACTAACCGT GTCACACTCC CACCCACACC CCCACGCACA CTCCCAAACC 
CACCCCGGAC ACGTGCACAC GCCCCACCCA GCCAGCCAAT CCACGCACCA AACACACTTT 
ATATTCGGCA CTCTGCCCCA CAACCTTCCG TCCGCCTCCG TCAATGCGCA TCACGCAGCC 
TCCGCCGCCC CGTATCTGCC CCAGTACCCT CCTCTCTCCT CCATCCCACC TCCCCCACCG 
CACTCCCCTT TACCCCCCCC TTCGTCCCCC CTTACCCCTC TTACCCCTCT CTCCCCTCAC 
CCCCCCCAGC ACCCCGGGCA GCCTCAGCAG GGGCCACAGG TGACTGGGGC TGGAACGACA 
GGTACAGGGG GCAGCTCCAA ATCCAAGCCT ATCAACCGGA TAAGCACCGT GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101076483 ( XM_011602739.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011601041.2 Takifugu rubripes IQ motif and SEC7 domain-containing protein 2-like (LOC101076483), transcript variant X1, mRNA.

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CloneID	OTo63423
Clone ID Related Accession (Same CDS sequence)	XM_011602739.2
Accession Version	XM_011602739.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4617bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	IQ motif and SEC7 domain-containing protein 2-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042287.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011602739.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAACA GCACAGAAAG CCCCAACAGG GCGGTGGAGT ATCTCCTGGA GCTCAACAAC 
ATCATCGAGA ACCAGGCCAA GCTCCTGGAG ACCCAGCGCA GACGCATCGA GGAGCTGGAG 
GGCCAGCTGG ACCGCGTCAG CCAGGAGAAC CAGGACCTGA AGCAGGAGCG GAGCCCGCGC 
ACGCCCGGCT CGGACACACC GGAGCAGGAC CACAACCACA GCCAGCCTCT GTCGCTCCCC 
ATCCATCACG GCGGCGGCGC CTCGGCGGCG CAGGTGACGG TCAGCTCGGC CACGACTCCG 
GGGCCCAGCA GGGAGAGGAG GAGCCACACC AGGCTGACCC GGGGGCTCTC CTGCGGCTCG 
TCCACCACCG ACCGAGACCG GCTTGAGCGC ACCGACAGCA CCGACACCAA CAACAGCGCC 
ACCATCCGCC GAAATGTGGA AGGGGAGTCC CAGTTCCCAG ATGTGGGCGC CCGCAAAGAC 
AGCTCCTTCA GCAAGACCCT TTACCGCTCC GAACGCTACG GCGACAGCAG CAGCAGCAGC 
GCACCCTCGG GGTCCAGGGA CCCCTCGTGC GTGGCCCCCT CGAATTCAGC CAGCTTGGCC 
TGGGCGCTCC GAACACGGCA CCAGCCGGCG AGCCTTGCCC TCCGTAAGCA AGAGGAAGAG 
GAGCACAAGA GGTGCAAGGC TCTCTCAGAC AGCTATGAGC TGTCAACAGA TCTGCAGGAT 
AAGAAGGTGG AGATGCTGGA GAGAAAGTAC GGCGGCTCCT TCGTAAGCCG CAGGGCAGCT 
CGAACCATCC AGACGGCGTT CAGGCAGTAC CGCATGAACA AGAACTTTGA ACGCCTCAGG 
AGTTCCGCTT CGGAGAGCCG CATGACTCGG CGCATCATCC TGTCCAACAT GCGACTGCAG 
TACTCCTTTG ACGAGCGGCA GCCGCCACAG CAGGCCCAGA CGCAGACCAA CTTCACCCAC 
AGCGTGGCCA TAGGACCGCC TCACTCCCCC GACACGGACC GTCCGGGAGA CTACACCCAG 
TTAGAGGATT CCTTCTCCAA ACAGGTCAAG TCTTTAGCCG ACTCCATAGA CGATGCTCTC 
ACCTGCCGTG GGGGTCGTAA CGACTCCCAG GAGGGCAGCA GGGAGGGTGG CGGGATTAGC 
GAGGACTTCG GGGAGTGTGT GTGGAGCAGC AGCAGCAACC CCTCCTCCCA AAGAGGATTG 
GGAGAACGCG CCAGGGGGGC AGGAGGAGGA CTCAGCATGC ACGGCGACAG CACCGCCACC 
TCGTACAGCG ACGTCACCCT GTACATGGAT GACGGCATGC CTTCATCGCC CCTGTCCCTG 
GACCGAGCGC CCAGCAGCAC AGACACAGAG TACTGGGGCC CGGGCGGGGG TGTCGGAGGG 
CGCGAGGACA GCAGGGACAC CGAAGGAGGG GGCAGCAGCA ACAGTCGGCG CAGCACCCCG 
TGCACCGAGT GCAGAGACTT CCGTTTTAGA GGCGCCCATC TGCCTCTCCT CACCATCGAG 
CCCCCGAGTG ACAGCTCGGT AGACATGAGC GACCGCTCGG ATCGGGGTTC TTTGAGCAGA 
CAGCTGGTCT ATGAGCAGGA GCCGGGGGTC GGGGTGGGCT CCCCTCAGGG AACCCTCAAA 
CACTCCACCA ACACGGCCAC CCGTGCTTCC TCCACAGCTG CTGCCCAGGG TCAGACTCGG 
GCCCCCGGCA GACCCTTACC CACGCACATC CCGCACCAGG TGGCGGCTCA CCACCACCAT 
CACCACCACC CGGTCCACCA CCATCAGTAC CCCGACACGC CCTCCTCGTC CTCATCGCCC 
CAGCAGCCCC CCACCACCCC TCTGTCCTCC TCCTCGTCCA CCGCTCTGCC TCCCGGTGGG 
CTGGAGCAGC CGTGCTGCTC GGACGGAGAC AACGACTCGC TCAACTCCAC CACTAACTCC 
AACGAGACGG TGAACTGCAG CTCCGGTTCC TCCTCCCAGG ACAGCCTGCA GGAGCCTCTG 
CCACCTCTGG GGAAGCAGAC GTATCAGAGG GAAACGCGCC ACAGCTGGGA CTCGCCGCCC 
TTCAACAGCG ATGTGGTGCA GAGACGCCAG TACCGCATAG GCCTTAACCT CTTCAACAAG 
AAGCCAGAAA AGGGAATCCA GTACTTGATA GAGAGGGGAT TCGTATCAGA CACTCCAGTT 
GGAATTGCTC GTTTCATCCT GGAGAGGAAG GGCCTGAGCC GCCAGATGAT TGGAGAGTTC 
CTGGGAAACC GACAGAAACA GTTCAACAAG GATGTTTTGG ACTGTGTGGT GGATGAAATG 
GACTTCTCAG GTATGGACCT GGATGATGCT CTTCGAAAAT TCCAGGCCCA GATTAAGGTT 
CAAGGAGAAG CCCAGAAAGT GGAGCGGCTC ATAGAGGCTT TTAGTCAGAG ATACTGCGTT 
TGTAATCCGG CTCTGGTTCG CCAGTTCCAA AACCCAGATA CCATCTTCAT CCTGGCCTTT 
GCCATAATCC TGCTGAACAC AGACATGTAC AGTCCCAACG TCAAAGCCGA GCGGAAGATG 
AAACTAGAAG ATTTTATAAA GAATTTAAGG GGTGTGGATA ATGGTCAGGA CATACCCAGG 
GACCTGTTGG TCGGGATCTA CCAGAGAATA CAGAAGTGGG AGCTGCGGAC CAACGACGAT 
CACGTTTCAC AAGTGCAGGC AGTCGAGAGA GTCATTGTGG GCAAAAAGCC TGTGCTGTCC 
CTTCCCCATC GCCGGCTGGT GTGTTGCTGC CAGCTTTACG AAGTTCCGGA CCCCAACCGA 
CCTCAAAGGA CGGGGGTGCA CCAACGGGAG GTCTTTCTCT TTAACGACCT TCTGGTGGTG 
ACCAAAATCT TCCAGAAGAA GAAAACTTCT GTCACCTACA GTTTCAGACA GTCCTTCTCT 
TTGGTTGAGA TGCAAGTGCA AATGTTCCAA AACTCATACT ACCCTCATGG TATTCGTCTG 
ACCTCGGCGA TCCTGGGCGG CGAGAGGAAG GTTCTCATCG TCTTTATGGC GCCCAGTCAA 
CAAGACCGCA CTCGCTTCGT CAGTGACCTC AGGGAGAGTA TTGCTGAAGT TCAAGAGATG 
GAGAAATATA GGGTTGAATC GGAGCTGGAA AAGCAAAAAG GTGTGATGCG GCCCAGTTTG 
CTGACTGGAG GCGTGGTAGG AGGAGGCGTG GGTGTGAAGG GCGACGTGGT GAACGGCACC 
CTGGGAAGGA CGAGTTTTGA CGACAACTGT GAGGGGCTGA AACGCACCGC GCTCAGCTCC 
TCTCTCAGGG ACCTCTCGGA CACGGGGAAA CATGGTCGCA GGAACAGTGT TGGTTCTCTG 
GATAGTACCA TGGAAGGCTC CATCATTAGC AGTCCCCAGC CTCACCAGCG ATACCCACTG 
CCGGGAGTGA TGCCGGGTGT GATGCCAGGT GTGCTGTCGG GTGTGATGCC GGGTGTGATG 
CCGGGTGTGA TGCCGGGTGT GATGCCGGGT GTGATGCCAG GTGTGATGCC GGGTGTTCTC 
CCAGGTGTGA TCCCGGGATG CTATGGAACA GATGACTTCC GGTCTCATCG CCCCATCCTG 
AGCCCCGGGA CGGGGGTGGG GGGTGGGCCG GGGCAGCAAA ATACCACTGC TGGGACAGAA 
GTTGGGGGAG TCTCCAGCAG TGTAGAGAGA GCAGGAGCGG GGAGCAACCC TGGGGGGAGC 
AGCAGCAACA ACAGCAGCTC TTTTCTGGGA TCCCTGTTTG GCAGCAAACG TGCCAAACCG 
CCAGGGCCCC TTATTCCACC GGGGCCACCC TTCCCCGCAC CTGTCCCCCC GCCGACTTCG 
GGAGGGCCCC CTCCTCACTC CCCTTCATCT CTCTGTCAGA TGGACGGGGG ACCTTCCAAG 
ATCCAAGCGC TGCATGCGCA GTACTGTCAC ACGGGCGCCG TGCAGCCTCC ACCACCGTAC 
TACCATCACC ATCGCTTCCA CGTCCAGCCT GTCCAGGCCC CTTTGCAAGG CCTACCCCCC 
CATACTATAC AGAGAGGCCC GCTACCCTGT CGGCCAGTGC TTGGCCCCCC ACACACCCAG 
CACCTGCAGC TGGCCCATCT CTCCCAGCTC TCCCAGCCTG GCCTTCCGGG TCGCTACGGC 
AACCTGGTGT TGGGATGTCC TCCCCCTCTT TCTCCTCTCT CCCAACATTC CCAGAACCCC 
CAGTGCGCCC TCTACCACGT TCAGCCCACA CACTCTATCA GAGGGGGCGG CGGTCCCGGT 
ACCAAACTAC CACTAACCGT GTCACACTCC CACCCACACC CCCACGCACA CTCCCAAACC 
CACCCCGGAC ACGTGCACAC GCCCCACCCA GCCAGCCAAT CCACGCACCA AACACACTTT 
ATATTCGGCA CTCTGCCCCA CAACCTTCCG TCCGCCTCCG TCAATGCGCA TCACGCAGCC 
TCCGCCGCCC CGTATCTGCC CCAGTACCCT CCTCTCTCCT CCATCCCACC TCCCCCACCG 
CACTCCCCTT TACCCCCCCC TTCGTCCCCC CTTACCCCTC TTACCCCTCT CTCCCCTCAC 
CCCCCCCAGC ACCCCGGGCA GCCTCAGCAG GGGCCACAGG TGACTGGGGC TGGAACGACA 
GGTACAGGGG GCAGCTCCAA ATCCAAGCCT ATCAACCGGA TAAGCACCGT GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011601041.2
CDS	1213..5829
Translation	MENSTESPNRAVEYLLELNNIIENQAKLLETQRRRIEELEGQLDRVSQENQDLKQERSPRTPGSDTPEQDHNHSQPLSLPIHHGGGASAAQVTVSSATTPGPSRERRSHTRLTRGLSCGSSTTDRDRLERTDSTDTNNSATIRRNVEGESQFPDVGARKDSSFSKTLYRSERYGDSSSSSAPSGSRDPSCVAPSNSASLAWALRTRHQPASLALRKQEEEEHKRCKALSDSYELSTDLQDKKVEMLERKYGGSFVSRRAARTIQTAFRQYRMNKNFERLRSSASESRMTRRIILSNMRLQYSFDERQPPQQAQTQTNFTHSVAIGPPHSPDTDRPGDYTQLEDSFSKQVKSLADSIDDALTCRGGRNDSQEGSREGGGISEDFGECVWSSSSNPSSQRGLGERARGAGGGLSMHGDSTATSYSDVTLYMDDGMPSSPLSLDRAPSSTDTEYWGPGGGVGGREDSRDTEGGGSSNSRRSTPCTECRDFRFRGAHLPLLTIEPPSDSSVDMSDRSDRGSLSRQLVYEQEPGVGVGSPQGTLKHSTNTATRASSTAAAQGQTRAPGRPLPTHIPHQVAAHHHHHHHPVHHHQYPDTPSSSSSPQQPPTTPLSSSSSTALPPGGLEQPCCSDGDNDSLNSTTNSNETVNCSSGSSSQDSLQEPLPPLGKQTYQRETRHSWDSPPFNSDVVQRRQYRIGLNLFNKKPEKGIQYLIERGFVSDTPVGIARFILERKGLSRQMIGEFLGNRQKQFNKDVLDCVVDEMDFSGMDLDDALRKFQAQIKVQGEAQKVERLIEAFSQRYCVCNPALVRQFQNPDTIFILAFAIILLNTDMYSPNVKAERKMKLEDFIKNLRGVDNGQDIPRDLLVGIYQRIQKWELRTNDDHVSQVQAVERVIVGKKPVLSLPHRRLVCCCQLYEVPDPNRPQRTGVHQREVFLFNDLLVVTKIFQKKKTSVTYSFRQSFSLVEMQVQMFQNSYYPHGIRLTSAILGGERKVLIVFMAPSQQDRTRFVSDLRESIAEVQEMEKYRVESELEKQKGVMRPSLLTGGVVGGGVGVKGDVVNGTLGRTSFDDNCEGLKRTALSSSLRDLSDTGKHGRRNSVGSLDSTMEGSIISSPQPHQRYPLPGVMPGVMPGVLSGVMPGVMPGVMPGVMPGVMPGVMPGVLPGVIPGCYGTDDFRSHRPILSPGTGVGGGPGQQNTTAGTEVGGVSSSVERAGAGSNPGGSSSNNSSSFLGSLFGSKRAKPPGPLIPPGPPFPAPVPPPTSGGPPPHSPSSLCQMDGGPSKIQALHAQYCHTGAVQPPPPYYHHHRFHVQPVQAPLQGLPPHTIQRGPLPCRPVLGPPHTQHLQLAHLSQLSQPGLPGRYGNLVLGCPPPLSPLSQHSQNPQCALYHVQPTHSIRGGGGPGTKLPLTVSHSHPHPHAHSQTHPGHVHTPHPASQSTHQTHFIFGTLPHNLPSASVNAHHAASAAPYLPQYPPLSSIPPPPPHSPLPPPSSPLTPLTPLSPHPPQHPGQPQQGPQVTGAGTTGTGGSSKSKPINRISTVV

Target ORF information:

RefSeq Version	XM_011602739.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes IQ motif and SEC7 domain-containing protein 2-like (LOC101076483), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011602739.2

ORF Insert Sequence:

ATGGAGAACA GCACAGAAAG CCCCAACAGG GCGGTGGAGT ATCTCCTGGA GCTCAACAAC 
ATCATCGAGA ACCAGGCCAA GCTCCTGGAG ACCCAGCGCA GACGCATCGA GGAGCTGGAG 
GGCCAGCTGG ACCGCGTCAG CCAGGAGAAC CAGGACCTGA AGCAGGAGCG GAGCCCGCGC 
ACGCCCGGCT CGGACACACC GGAGCAGGAC CACAACCACA GCCAGCCTCT GTCGCTCCCC 
ATCCATCACG GCGGCGGCGC CTCGGCGGCG CAGGTGACGG TCAGCTCGGC CACGACTCCG 
GGGCCCAGCA GGGAGAGGAG GAGCCACACC AGGCTGACCC GGGGGCTCTC CTGCGGCTCG 
TCCACCACCG ACCGAGACCG GCTTGAGCGC ACCGACAGCA CCGACACCAA CAACAGCGCC 
ACCATCCGCC GAAATGTGGA AGGGGAGTCC CAGTTCCCAG ATGTGGGCGC CCGCAAAGAC 
AGCTCCTTCA GCAAGACCCT TTACCGCTCC GAACGCTACG GCGACAGCAG CAGCAGCAGC 
GCACCCTCGG GGTCCAGGGA CCCCTCGTGC GTGGCCCCCT CGAATTCAGC CAGCTTGGCC 
TGGGCGCTCC GAACACGGCA CCAGCCGGCG AGCCTTGCCC TCCGTAAGCA AGAGGAAGAG 
GAGCACAAGA GGTGCAAGGC TCTCTCAGAC AGCTATGAGC TGTCAACAGA TCTGCAGGAT 
AAGAAGGTGG AGATGCTGGA GAGAAAGTAC GGCGGCTCCT TCGTAAGCCG CAGGGCAGCT 
CGAACCATCC AGACGGCGTT CAGGCAGTAC CGCATGAACA AGAACTTTGA ACGCCTCAGG 
AGTTCCGCTT CGGAGAGCCG CATGACTCGG CGCATCATCC TGTCCAACAT GCGACTGCAG 
TACTCCTTTG ACGAGCGGCA GCCGCCACAG CAGGCCCAGA CGCAGACCAA CTTCACCCAC 
AGCGTGGCCA TAGGACCGCC TCACTCCCCC GACACGGACC GTCCGGGAGA CTACACCCAG 
TTAGAGGATT CCTTCTCCAA ACAGGTCAAG TCTTTAGCCG ACTCCATAGA CGATGCTCTC 
ACCTGCCGTG GGGGTCGTAA CGACTCCCAG GAGGGCAGCA GGGAGGGTGG CGGGATTAGC 
GAGGACTTCG GGGAGTGTGT GTGGAGCAGC AGCAGCAACC CCTCCTCCCA AAGAGGATTG 
GGAGAACGCG CCAGGGGGGC AGGAGGAGGA CTCAGCATGC ACGGCGACAG CACCGCCACC 
TCGTACAGCG ACGTCACCCT GTACATGGAT GACGGCATGC CTTCATCGCC CCTGTCCCTG 
GACCGAGCGC CCAGCAGCAC AGACACAGAG TACTGGGGCC CGGGCGGGGG TGTCGGAGGG 
CGCGAGGACA GCAGGGACAC CGAAGGAGGG GGCAGCAGCA ACAGTCGGCG CAGCACCCCG 
TGCACCGAGT GCAGAGACTT CCGTTTTAGA GGCGCCCATC TGCCTCTCCT CACCATCGAG 
CCCCCGAGTG ACAGCTCGGT AGACATGAGC GACCGCTCGG ATCGGGGTTC TTTGAGCAGA 
CAGCTGGTCT ATGAGCAGGA GCCGGGGGTC GGGGTGGGCT CCCCTCAGGG AACCCTCAAA 
CACTCCACCA ACACGGCCAC CCGTGCTTCC TCCACAGCTG CTGCCCAGGG TCAGACTCGG 
GCCCCCGGCA GACCCTTACC CACGCACATC CCGCACCAGG TGGCGGCTCA CCACCACCAT 
CACCACCACC CGGTCCACCA CCATCAGTAC CCCGACACGC CCTCCTCGTC CTCATCGCCC 
CAGCAGCCCC CCACCACCCC TCTGTCCTCC TCCTCGTCCA CCGCTCTGCC TCCCGGTGGG 
CTGGAGCAGC CGTGCTGCTC GGACGGAGAC AACGACTCGC TCAACTCCAC CACTAACTCC 
AACGAGACGG TGAACTGCAG CTCCGGTTCC TCCTCCCAGG ACAGCCTGCA GGAGCCTCTG 
CCACCTCTGG GGAAGCAGAC GTATCAGAGG GAAACGCGCC ACAGCTGGGA CTCGCCGCCC 
TTCAACAGCG ATGTGGTGCA GAGACGCCAG TACCGCATAG GCCTTAACCT CTTCAACAAG 
AAGCCAGAAA AGGGAATCCA GTACTTGATA GAGAGGGGAT TCGTATCAGA CACTCCAGTT 
GGAATTGCTC GTTTCATCCT GGAGAGGAAG GGCCTGAGCC GCCAGATGAT TGGAGAGTTC 
CTGGGAAACC GACAGAAACA GTTCAACAAG GATGTTTTGG ACTGTGTGGT GGATGAAATG 
GACTTCTCAG GTATGGACCT GGATGATGCT CTTCGAAAAT TCCAGGCCCA GATTAAGGTT 
CAAGGAGAAG CCCAGAAAGT GGAGCGGCTC ATAGAGGCTT TTAGTCAGAG ATACTGCGTT 
TGTAATCCGG CTCTGGTTCG CCAGTTCCAA AACCCAGATA CCATCTTCAT CCTGGCCTTT 
GCCATAATCC TGCTGAACAC AGACATGTAC AGTCCCAACG TCAAAGCCGA GCGGAAGATG 
AAACTAGAAG ATTTTATAAA GAATTTAAGG GGTGTGGATA ATGGTCAGGA CATACCCAGG 
GACCTGTTGG TCGGGATCTA CCAGAGAATA CAGAAGTGGG AGCTGCGGAC CAACGACGAT 
CACGTTTCAC AAGTGCAGGC AGTCGAGAGA GTCATTGTGG GCAAAAAGCC TGTGCTGTCC 
CTTCCCCATC GCCGGCTGGT GTGTTGCTGC CAGCTTTACG AAGTTCCGGA CCCCAACCGA 
CCTCAAAGGA CGGGGGTGCA CCAACGGGAG GTCTTTCTCT TTAACGACCT TCTGGTGGTG 
ACCAAAATCT TCCAGAAGAA GAAAACTTCT GTCACCTACA GTTTCAGACA GTCCTTCTCT 
TTGGTTGAGA TGCAAGTGCA AATGTTCCAA AACTCATACT ACCCTCATGG TATTCGTCTG 
ACCTCGGCGA TCCTGGGCGG CGAGAGGAAG GTTCTCATCG TCTTTATGGC GCCCAGTCAA 
CAAGACCGCA CTCGCTTCGT CAGTGACCTC AGGGAGAGTA TTGCTGAAGT TCAAGAGATG 
GAGAAATATA GGGTTGAATC GGAGCTGGAA AAGCAAAAAG GTGTGATGCG GCCCAGTTTG 
CTGACTGGAG GCGTGGTAGG AGGAGGCGTG GGTGTGAAGG GCGACGTGGT GAACGGCACC 
CTGGGAAGGA CGAGTTTTGA CGACAACTGT GAGGGGCTGA AACGCACCGC GCTCAGCTCC 
TCTCTCAGGG ACCTCTCGGA CACGGGGAAA CATGGTCGCA GGAACAGTGT TGGTTCTCTG 
GATAGTACCA TGGAAGGCTC CATCATTAGC AGTCCCCAGC CTCACCAGCG ATACCCACTG 
CCGGGAGTGA TGCCGGGTGT GATGCCAGGT GTGCTGTCGG GTGTGATGCC GGGTGTGATG 
CCGGGTGTGA TGCCGGGTGT GATGCCGGGT GTGATGCCAG GTGTGATGCC GGGTGTTCTC 
CCAGGTGTGA TCCCGGGATG CTATGGAACA GATGACTTCC GGTCTCATCG CCCCATCCTG 
AGCCCCGGGA CGGGGGTGGG GGGTGGGCCG GGGCAGCAAA ATACCACTGC TGGGACAGAA 
GTTGGGGGAG TCTCCAGCAG TGTAGAGAGA GCAGGAGCGG GGAGCAACCC TGGGGGGAGC 
AGCAGCAACA ACAGCAGCTC TTTTCTGGGA TCCCTGTTTG GCAGCAAACG TGCCAAACCG 
CCAGGGCCCC TTATTCCACC GGGGCCACCC TTCCCCGCAC CTGTCCCCCC GCCGACTTCG 
GGAGGGCCCC CTCCTCACTC CCCTTCATCT CTCTGTCAGA TGGACGGGGG ACCTTCCAAG 
ATCCAAGCGC TGCATGCGCA GTACTGTCAC ACGGGCGCCG TGCAGCCTCC ACCACCGTAC 
TACCATCACC ATCGCTTCCA CGTCCAGCCT GTCCAGGCCC CTTTGCAAGG CCTACCCCCC 
CATACTATAC AGAGAGGCCC GCTACCCTGT CGGCCAGTGC TTGGCCCCCC ACACACCCAG 
CACCTGCAGC TGGCCCATCT CTCCCAGCTC TCCCAGCCTG GCCTTCCGGG TCGCTACGGC 
AACCTGGTGT TGGGATGTCC TCCCCCTCTT TCTCCTCTCT CCCAACATTC CCAGAACCCC 
CAGTGCGCCC TCTACCACGT TCAGCCCACA CACTCTATCA GAGGGGGCGG CGGTCCCGGT 
ACCAAACTAC CACTAACCGT GTCACACTCC CACCCACACC CCCACGCACA CTCCCAAACC 
CACCCCGGAC ACGTGCACAC GCCCCACCCA GCCAGCCAAT CCACGCACCA AACACACTTT 
ATATTCGGCA CTCTGCCCCA CAACCTTCCG TCCGCCTCCG TCAATGCGCA TCACGCAGCC 
TCCGCCGCCC CGTATCTGCC CCAGTACCCT CCTCTCTCCT CCATCCCACC TCCCCCACCG 
CACTCCCCTT TACCCCCCCC TTCGTCCCCC CTTACCCCTC TTACCCCTCT CTCCCCTCAC 
CCCCCCCAGC ACCCCGGGCA GCCTCAGCAG GGGCCACAGG TGACTGGGGC TGGAACGACA 
GGTACAGGGG GCAGCTCCAA ATCCAAGCCT ATCAACCGGA TAAGCACCGT GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101076483 ( XM_029833497.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029689357.1 Takifugu rubripes IQ motif and SEC7 domain-containing protein 2-like (LOC101076483), transcript variant X2, mRNA.

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CloneID	OTo63424
Clone ID Related Accession (Same CDS sequence)	XM_029833497.1
Accession Version	XM_029833497.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	IQ motif and SEC7 domain-containing protein 2-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042287.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAACA GCACAGAAAG CCCCAACAGG GCGGTGGAGT ATCTCCTGGA GCTCAACAAC 
ATCATCGAGA ACCAGGCCAA GCTCCTGGAG ACCCAGCGCA GACGCATCGA GGAGCTGGAG 
GGCCAGCTGG ACCGCGTCAG CCAGGAGAAC CAGGACCTGA AGCAGGAGCG GAGCCCGCGC 
ACGCCCGGCT CGGACACACC GGAGCAGGAC CACAACCACA GCCAGCCTCT GTCGCTCCCC 
ATCCATCACG GCGGCGGCGC CTCGGCGGCG CAGGTGACGG TCAGCTCGGC CACGACTCCG 
GGGCCCAGCA GGGAGAGGAG GAGCCACACC AGGCTGACCC GGGGGCTCTC CTGCGGCTCG 
TCCACCACCG ACCGAGACCG GCTTGAGCGC ACCGACAGCA CCGACACCAA CAACAGCGCC 
ACCATCCGCC GAAATGTGGA AGGGGAGTCC CAGTTCCCAG ATGTGGGCGC CCGCAAAGAC 
AGCTCCTTCA GCAAGACCCT TTACCGCTCC GAACGCTACG GCGACAGCAG CAGCAGCAGC 
GCACCCTCGG GGTCCAGGGA CCCCTCGTGC GTGGCCCCCT CGAATTCAGC CAGCTTGGCC 
TGGGCGCTCC GAACACGGCA CCAGCCGGCG AGCCTTGCCC TCCGTAAGCA AGAGGAAGAG 
GAGCACAAGA GGTGCAAGGC TCTCTCAGAC AGCTATGAGC TGTCAACAGA TCTGCAGGAT 
AAGAAGGTGG AGATGCTGGA GAGAAAGTAC GGCGGCTCCT TCGTAAGCCG CAGGGCAGCT 
CGAACCATCC AGACGGCGTT CAGGCAGTAC CGCATGAACA AGAACTTTGA ACGCCTCAGG 
AGTTCCGCTT CGGAGAGCCG CATGACTCGG CGCATCATCC TGTCCAACAT GCGACTGCAG 
TACTCCTTTG ACGAGCGGCA GCCGCCACAG CAGGCCCAGA CGCAGACCAA CTTCACCCAC 
AGCGTGGCCA TAGGACCGCC TCACTCCCCC GACACGGACC GTCCGGGAGA CTACACCCAG 
TTAGAGGATT CCTTCTCCAA ACAGGTCAAG TCTTTAGCCG ACTCCATAGA CGATGCTCTC 
ACCTGCCGTG GGGGTCGTAA CGACTCCCAG GAGGGCAGCA GGGAGGGTGG CGGGATTAGC 
GAGGACTTCG GGGAGTGTGT GTGGAGCAGC AGCAGCAACC CCTCCTCCCA AAGAGGATTG 
GGAGAACGCG CCAGGGGGGC AGGAGGAGGA CTCAGCATGC ACGGCGACAG CACCGCCACC 
TCGTACAGCG ACGTCACCCT GTACATGGAT GACGGCATGC CTTCATCGCC CCTGTCCCTG 
GACCGAGCGC CCAGCAGCAC AGACACAGAG TACTGGGGCC CGGGCGGGGG TGTCGGAGGG 
CGCGAGGACA GCAGGGACAC CGAAGGAGGG GGCAGCAGCA ACAGTCGGCG CAGCACCCCG 
TGCACCGAGT GCAGAGACTT CCGTTTTAGA GGCGCCCATC TGCCTCTCCT CACCATCGAG 
CCCCCGAGTG ACAGCTCGGT AGACATGAGC GACCGCTCGG ATCGGGGTTC TTTGAGCAGA 
CAGCTGGTCT ATGAGCAGGA GCCGGGGGTC GGGGTGGGCT CCCCTCAGGG AACCCTCAAA 
CACTCCACCA ACACGGCCAC CCGTGCTTCC TCCACAGCTG CTGCCCAGGG TCAGACTCGG 
GCCCCCGGCA GACCCTTACC CACGCACATC CCGCACCAGG TGGCGGCTCA CCACCACCAT 
CACCACCACC CGGTCCACCA CCATCAGTAC CCCGACACGC CCTCCTCGTC CTCATCGCCC 
CAGCAGCCCC CCACCACCCC TCTGTCCTCC TCCTCGTCCA CCGCTCTGCC TCCCGGTGGG 
CTGGAGCAGC CGTGCTGCTC GGACGGAGAC AACGACTCGC TCAACTCCAC CACTAACTCC 
AACGAGACGG TGAACTGCAG CTCCGGTTCC TCCTCCCAGG ACAGCCTGCA GGAGCCTCTG 
CCACCTCTGG GGAAGCAGAC GTATCAGAGG GAAACGCGCC ACAGCTGGGA CTCGCCGCCC 
TTCAACAGCG ATGTGGTGCA GAGACGCCAG TACCGCATAG GCCTTAACCT CTTCAACAAG 
AAGCCAGAAA AGGGAATCCA GTACTTGATA GAGAGGGGAT TCGTATCAGA CACTCCAGTT 
GGAATTGCTC GTTTCATCCT GGAGAGGAAG GGCCTGAGCC GCCAGATGAT TGGAGAGTTC 
CTGGGAAACC GACAGAAACA GTTCAACAAG GATGTTTTGG ACTGTGTGGT GGATGAAATG 
GACTTCTCAG GTATGGACCT GGATGATGCT CTTCGAAAAT TCCAGGCCCA GATTAAGGTT 
CAAGGAGAAG CCCAGAAAGT GGAGCGGCTC ATAGAGGCTT TTAGTCAGAG ATACTGCGTT 
TGTAATCCGG CTCTGGTTCG CCAGTTCCAA AACCCAGATA CCATCTTCAT CCTGGCCTTT 
GCCATAATCC TGCTGAACAC AGACATGTAC AGTCCCAACG TCAAAGCCGA GCGGAAGATG 
AAACTAGAAG ATTTTATAAA GAATTTAAGG GGTGTGGATA ATGGTCAGGA CATACCCAGG 
GACCTGTTGG TCGGGATCTA CCAGAGAATA CAGAAGTGGG AGCTGCGGAC CAACGACGAT 
CACGTTTCAC AAGTGCAGGC AGTCGAGAGA GTCATTGTGC TGTCCCTTCC CCATCGCCGG 
CTGGTGTGTT GCTGCCAGCT TTACGAAGTT CCGGACCCCA ACCGACCTCA AAGGACGGGG 
GTGCACCAAC GGGAGGTCTT TCTCTTTAAC GACCTTCTGG TGGTGACCAA AATCTTCCAG 
AAGAAGAAAA CTTCTGTCAC CTACAGTTTC AGACAGTCCT TCTCTTTGGT TGAGATGCAA 
GTGCAAATGT TCCAAAACTC ATACTACCCT CATGGTATTC GTCTGACCTC GGCGATCCTG 
GGCGGCGAGA GGAAGGTTCT CATCGTCTTT ATGGCGCCCA GTCAACAAGA CCGCACTCGC 
TTCGTCAGTG ACCTCAGGGA GAGTATTGCT GAAGTTCAAG AGATGGAGAA ATATAGGGTT 
GAATCGGAGC TGGAAAAGCA AAAAGGTGTG ATGCGGCCCA GTTTGCTGAC TGGAGGCGTG 
GTAGGAGGAG GCGTGGGTGT GAAGGGCGAC GTGGTGAACG GCACCCTGGG AAGGACGAGT 
TTTGACGACA ACTGTGAGGG GCTGAAACGC ACCGCGCTCA GCTCCTCTCT CAGGGACCTC 
TCGGACACGG GGAAACATGG TCGCAGGAAC AGTGTTGGTT CTCTGGATAG TACCATGGAA 
GGCTCCATCA TTAGCAGTCC CCAGCCTCAC CAGCGATACC CACTGCCGGG AGTGATGCCG 
GGTGTGATGC CAGGTGTGCT GTCGGGTGTG ATGCCGGGTG TGATGCCGGG TGTGATGCCG 
GGTGTGATGC CGGGTGTGAT GCCAGGTGTG ATGCCGGGTG TTCTCCCAGG TGTGATCCCG 
GGATGCTATG GAACAGATGA CTTCCGGTCT CATCGCCCCA TCCTGAGCCC CGGGACGGGG 
GTGGGGGGTG GGCCGGGGCA GCAAAATACC ACTGCTGGGA CAGAAGTTGG GGGAGTCTCC 
AGCAGTGTAG AGAGAGCAGG AGCGGGGAGC AACCCTGGGG GGAGCAGCAG CAACAACAGC 
AGCTCTTTTC TGGGATCCCT GTTTGGCAGC AAACGTGCCA AACCGCCAGG GCCCCTTATT 
CCACCGGGGC CACCCTTCCC CGCACCTGTC CCCCCGCCGA CTTCGGGAGG GCCCCCTCCT 
CACTCCCCTT CATCTCTCTG TCAGATGGAC GGGGGACCTT CCAAGATCCA AGCGCTGCAT 
GCGCAGTACT GTCACACGGG CGCCGTGCAG CCTCCACCAC CGTACTACCA TCACCATCGC 
TTCCACGTCC AGCCTGTCCA GGCCCCTTTG CAAGGCCTAC CCCCCCATAC TATACAGAGA 
GGCCCGCTAC CCTGTCGGCC AGTGCTTGGC CCCCCACACA CCCAGCACCT GCAGCTGGCC 
CATCTCTCCC AGCTCTCCCA GCCTGGCCTT CCGGGTCGCT ACGGCAACCT GGTGTTGGGA 
TGTCCTCCCC CTCTTTCTCC TCTCTCCCAA CATTCCCAGA ACCCCCAGTG CGCCCTCTAC 
CACGTTCAGC CCACACACTC TATCAGAGGG GGCGGCGGTC CCGGTACCAA ACTACCACTA 
ACCGTGTCAC ACTCCCACCC ACACCCCCAC GCACACTCCC AAACCCACCC CGGACACGTG 
CACACGCCCC ACCCAGCCAG CCAATCCACG CACCAAACAC ACTTTATATT CGGCACTCTG 
CCCCACAACC TTCCGTCCGC CTCCGTCAAT GCGCATCACG CAGCCTCCGC CGCCCCGTAT 
CTGCCCCAGT ACCCTCCTCT CTCCTCCATC CCACCTCCCC CACCGCACTC CCCTTTACCC 
CCCCCTTCGT CCCCCCTTAC CCCTCTTACC CCTCTCTCCC CTCACCCCCC CCAGCACCCC 
GGGCAGCCTC AGCAGGGGCC ACAGGTGACT GGGGCTGGAA CGACAGGTAC AGGGGGCAGC 
TCCAAATCCA AGCCTATCAA CCGGATAAGC ACCGTGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029689357.1
CDS	1214..5815
Translation	MENSTESPNRAVEYLLELNNIIENQAKLLETQRRRIEELEGQLDRVSQENQDLKQERSPRTPGSDTPEQDHNHSQPLSLPIHHGGGASAAQVTVSSATTPGPSRERRSHTRLTRGLSCGSSTTDRDRLERTDSTDTNNSATIRRNVEGESQFPDVGARKDSSFSKTLYRSERYGDSSSSSAPSGSRDPSCVAPSNSASLAWALRTRHQPASLALRKQEEEEHKRCKALSDSYELSTDLQDKKVEMLERKYGGSFVSRRAARTIQTAFRQYRMNKNFERLRSSASESRMTRRIILSNMRLQYSFDERQPPQQAQTQTNFTHSVAIGPPHSPDTDRPGDYTQLEDSFSKQVKSLADSIDDALTCRGGRNDSQEGSREGGGISEDFGECVWSSSSNPSSQRGLGERARGAGGGLSMHGDSTATSYSDVTLYMDDGMPSSPLSLDRAPSSTDTEYWGPGGGVGGREDSRDTEGGGSSNSRRSTPCTECRDFRFRGAHLPLLTIEPPSDSSVDMSDRSDRGSLSRQLVYEQEPGVGVGSPQGTLKHSTNTATRASSTAAAQGQTRAPGRPLPTHIPHQVAAHHHHHHHPVHHHQYPDTPSSSSSPQQPPTTPLSSSSSTALPPGGLEQPCCSDGDNDSLNSTTNSNETVNCSSGSSSQDSLQEPLPPLGKQTYQRETRHSWDSPPFNSDVVQRRQYRIGLNLFNKKPEKGIQYLIERGFVSDTPVGIARFILERKGLSRQMIGEFLGNRQKQFNKDVLDCVVDEMDFSGMDLDDALRKFQAQIKVQGEAQKVERLIEAFSQRYCVCNPALVRQFQNPDTIFILAFAIILLNTDMYSPNVKAERKMKLEDFIKNLRGVDNGQDIPRDLLVGIYQRIQKWELRTNDDHVSQVQAVERVIVLSLPHRRLVCCCQLYEVPDPNRPQRTGVHQREVFLFNDLLVVTKIFQKKKTSVTYSFRQSFSLVEMQVQMFQNSYYPHGIRLTSAILGGERKVLIVFMAPSQQDRTRFVSDLRESIAEVQEMEKYRVESELEKQKGVMRPSLLTGGVVGGGVGVKGDVVNGTLGRTSFDDNCEGLKRTALSSSLRDLSDTGKHGRRNSVGSLDSTMEGSIISSPQPHQRYPLPGVMPGVMPGVLSGVMPGVMPGVMPGVMPGVMPGVMPGVLPGVIPGCYGTDDFRSHRPILSPGTGVGGGPGQQNTTAGTEVGGVSSSVERAGAGSNPGGSSSNNSSSFLGSLFGSKRAKPPGPLIPPGPPFPAPVPPPTSGGPPPHSPSSLCQMDGGPSKIQALHAQYCHTGAVQPPPPYYHHHRFHVQPVQAPLQGLPPHTIQRGPLPCRPVLGPPHTQHLQLAHLSQLSQPGLPGRYGNLVLGCPPPLSPLSQHSQNPQCALYHVQPTHSIRGGGGPGTKLPLTVSHSHPHPHAHSQTHPGHVHTPHPASQSTHQTHFIFGTLPHNLPSASVNAHHAASAAPYLPQYPPLSSIPPPPPHSPLPPPSSPLTPLTPLSPHPPQHPGQPQQGPQVTGAGTTGTGGSSKSKPINRISTVV

Target ORF information:

RefSeq Version	XM_029833497.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes IQ motif and SEC7 domain-containing protein 2-like (LOC101076483), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029833497.1

ORF Insert Sequence:

ATGGAGAACA GCACAGAAAG CCCCAACAGG GCGGTGGAGT ATCTCCTGGA GCTCAACAAC 
ATCATCGAGA ACCAGGCCAA GCTCCTGGAG ACCCAGCGCA GACGCATCGA GGAGCTGGAG 
GGCCAGCTGG ACCGCGTCAG CCAGGAGAAC CAGGACCTGA AGCAGGAGCG GAGCCCGCGC 
ACGCCCGGCT CGGACACACC GGAGCAGGAC CACAACCACA GCCAGCCTCT GTCGCTCCCC 
ATCCATCACG GCGGCGGCGC CTCGGCGGCG CAGGTGACGG TCAGCTCGGC CACGACTCCG 
GGGCCCAGCA GGGAGAGGAG GAGCCACACC AGGCTGACCC GGGGGCTCTC CTGCGGCTCG 
TCCACCACCG ACCGAGACCG GCTTGAGCGC ACCGACAGCA CCGACACCAA CAACAGCGCC 
ACCATCCGCC GAAATGTGGA AGGGGAGTCC CAGTTCCCAG ATGTGGGCGC CCGCAAAGAC 
AGCTCCTTCA GCAAGACCCT TTACCGCTCC GAACGCTACG GCGACAGCAG CAGCAGCAGC 
GCACCCTCGG GGTCCAGGGA CCCCTCGTGC GTGGCCCCCT CGAATTCAGC CAGCTTGGCC 
TGGGCGCTCC GAACACGGCA CCAGCCGGCG AGCCTTGCCC TCCGTAAGCA AGAGGAAGAG 
GAGCACAAGA GGTGCAAGGC TCTCTCAGAC AGCTATGAGC TGTCAACAGA TCTGCAGGAT 
AAGAAGGTGG AGATGCTGGA GAGAAAGTAC GGCGGCTCCT TCGTAAGCCG CAGGGCAGCT 
CGAACCATCC AGACGGCGTT CAGGCAGTAC CGCATGAACA AGAACTTTGA ACGCCTCAGG 
AGTTCCGCTT CGGAGAGCCG CATGACTCGG CGCATCATCC TGTCCAACAT GCGACTGCAG 
TACTCCTTTG ACGAGCGGCA GCCGCCACAG CAGGCCCAGA CGCAGACCAA CTTCACCCAC 
AGCGTGGCCA TAGGACCGCC TCACTCCCCC GACACGGACC GTCCGGGAGA CTACACCCAG 
TTAGAGGATT CCTTCTCCAA ACAGGTCAAG TCTTTAGCCG ACTCCATAGA CGATGCTCTC 
ACCTGCCGTG GGGGTCGTAA CGACTCCCAG GAGGGCAGCA GGGAGGGTGG CGGGATTAGC 
GAGGACTTCG GGGAGTGTGT GTGGAGCAGC AGCAGCAACC CCTCCTCCCA AAGAGGATTG 
GGAGAACGCG CCAGGGGGGC AGGAGGAGGA CTCAGCATGC ACGGCGACAG CACCGCCACC 
TCGTACAGCG ACGTCACCCT GTACATGGAT GACGGCATGC CTTCATCGCC CCTGTCCCTG 
GACCGAGCGC CCAGCAGCAC AGACACAGAG TACTGGGGCC CGGGCGGGGG TGTCGGAGGG 
CGCGAGGACA GCAGGGACAC CGAAGGAGGG GGCAGCAGCA ACAGTCGGCG CAGCACCCCG 
TGCACCGAGT GCAGAGACTT CCGTTTTAGA GGCGCCCATC TGCCTCTCCT CACCATCGAG 
CCCCCGAGTG ACAGCTCGGT AGACATGAGC GACCGCTCGG ATCGGGGTTC TTTGAGCAGA 
CAGCTGGTCT ATGAGCAGGA GCCGGGGGTC GGGGTGGGCT CCCCTCAGGG AACCCTCAAA 
CACTCCACCA ACACGGCCAC CCGTGCTTCC TCCACAGCTG CTGCCCAGGG TCAGACTCGG 
GCCCCCGGCA GACCCTTACC CACGCACATC CCGCACCAGG TGGCGGCTCA CCACCACCAT 
CACCACCACC CGGTCCACCA CCATCAGTAC CCCGACACGC CCTCCTCGTC CTCATCGCCC 
CAGCAGCCCC CCACCACCCC TCTGTCCTCC TCCTCGTCCA CCGCTCTGCC TCCCGGTGGG 
CTGGAGCAGC CGTGCTGCTC GGACGGAGAC AACGACTCGC TCAACTCCAC CACTAACTCC 
AACGAGACGG TGAACTGCAG CTCCGGTTCC TCCTCCCAGG ACAGCCTGCA GGAGCCTCTG 
CCACCTCTGG GGAAGCAGAC GTATCAGAGG GAAACGCGCC ACAGCTGGGA CTCGCCGCCC 
TTCAACAGCG ATGTGGTGCA GAGACGCCAG TACCGCATAG GCCTTAACCT CTTCAACAAG 
AAGCCAGAAA AGGGAATCCA GTACTTGATA GAGAGGGGAT TCGTATCAGA CACTCCAGTT 
GGAATTGCTC GTTTCATCCT GGAGAGGAAG GGCCTGAGCC GCCAGATGAT TGGAGAGTTC 
CTGGGAAACC GACAGAAACA GTTCAACAAG GATGTTTTGG ACTGTGTGGT GGATGAAATG 
GACTTCTCAG GTATGGACCT GGATGATGCT CTTCGAAAAT TCCAGGCCCA GATTAAGGTT 
CAAGGAGAAG CCCAGAAAGT GGAGCGGCTC ATAGAGGCTT TTAGTCAGAG ATACTGCGTT 
TGTAATCCGG CTCTGGTTCG CCAGTTCCAA AACCCAGATA CCATCTTCAT CCTGGCCTTT 
GCCATAATCC TGCTGAACAC AGACATGTAC AGTCCCAACG TCAAAGCCGA GCGGAAGATG 
AAACTAGAAG ATTTTATAAA GAATTTAAGG GGTGTGGATA ATGGTCAGGA CATACCCAGG 
GACCTGTTGG TCGGGATCTA CCAGAGAATA CAGAAGTGGG AGCTGCGGAC CAACGACGAT 
CACGTTTCAC AAGTGCAGGC AGTCGAGAGA GTCATTGTGC TGTCCCTTCC CCATCGCCGG 
CTGGTGTGTT GCTGCCAGCT TTACGAAGTT CCGGACCCCA ACCGACCTCA AAGGACGGGG 
GTGCACCAAC GGGAGGTCTT TCTCTTTAAC GACCTTCTGG TGGTGACCAA AATCTTCCAG 
AAGAAGAAAA CTTCTGTCAC CTACAGTTTC AGACAGTCCT TCTCTTTGGT TGAGATGCAA 
GTGCAAATGT TCCAAAACTC ATACTACCCT CATGGTATTC GTCTGACCTC GGCGATCCTG 
GGCGGCGAGA GGAAGGTTCT CATCGTCTTT ATGGCGCCCA GTCAACAAGA CCGCACTCGC 
TTCGTCAGTG ACCTCAGGGA GAGTATTGCT GAAGTTCAAG AGATGGAGAA ATATAGGGTT 
GAATCGGAGC TGGAAAAGCA AAAAGGTGTG ATGCGGCCCA GTTTGCTGAC TGGAGGCGTG 
GTAGGAGGAG GCGTGGGTGT GAAGGGCGAC GTGGTGAACG GCACCCTGGG AAGGACGAGT 
TTTGACGACA ACTGTGAGGG GCTGAAACGC ACCGCGCTCA GCTCCTCTCT CAGGGACCTC 
TCGGACACGG GGAAACATGG TCGCAGGAAC AGTGTTGGTT CTCTGGATAG TACCATGGAA 
GGCTCCATCA TTAGCAGTCC CCAGCCTCAC CAGCGATACC CACTGCCGGG AGTGATGCCG 
GGTGTGATGC CAGGTGTGCT GTCGGGTGTG ATGCCGGGTG TGATGCCGGG TGTGATGCCG 
GGTGTGATGC CGGGTGTGAT GCCAGGTGTG ATGCCGGGTG TTCTCCCAGG TGTGATCCCG 
GGATGCTATG GAACAGATGA CTTCCGGTCT CATCGCCCCA TCCTGAGCCC CGGGACGGGG 
GTGGGGGGTG GGCCGGGGCA GCAAAATACC ACTGCTGGGA CAGAAGTTGG GGGAGTCTCC 
AGCAGTGTAG AGAGAGCAGG AGCGGGGAGC AACCCTGGGG GGAGCAGCAG CAACAACAGC 
AGCTCTTTTC TGGGATCCCT GTTTGGCAGC AAACGTGCCA AACCGCCAGG GCCCCTTATT 
CCACCGGGGC CACCCTTCCC CGCACCTGTC CCCCCGCCGA CTTCGGGAGG GCCCCCTCCT 
CACTCCCCTT CATCTCTCTG TCAGATGGAC GGGGGACCTT CCAAGATCCA AGCGCTGCAT 
GCGCAGTACT GTCACACGGG CGCCGTGCAG CCTCCACCAC CGTACTACCA TCACCATCGC 
TTCCACGTCC AGCCTGTCCA GGCCCCTTTG CAAGGCCTAC CCCCCCATAC TATACAGAGA 
GGCCCGCTAC CCTGTCGGCC AGTGCTTGGC CCCCCACACA CCCAGCACCT GCAGCTGGCC 
CATCTCTCCC AGCTCTCCCA GCCTGGCCTT CCGGGTCGCT ACGGCAACCT GGTGTTGGGA 
TGTCCTCCCC CTCTTTCTCC TCTCTCCCAA CATTCCCAGA ACCCCCAGTG CGCCCTCTAC 
CACGTTCAGC CCACACACTC TATCAGAGGG GGCGGCGGTC CCGGTACCAA ACTACCACTA 
ACCGTGTCAC ACTCCCACCC ACACCCCCAC GCACACTCCC AAACCCACCC CGGACACGTG 
CACACGCCCC ACCCAGCCAG CCAATCCACG CACCAAACAC ACTTTATATT CGGCACTCTG 
CCCCACAACC TTCCGTCCGC CTCCGTCAAT GCGCATCACG CAGCCTCCGC CGCCCCGTAT 
CTGCCCCAGT ACCCTCCTCT CTCCTCCATC CCACCTCCCC CACCGCACTC CCCTTTACCC 
CCCCCTTCGT CCCCCCTTAC CCCTCTTACC CCTCTCTCCC CTCACCCCCC CCAGCACCCC 
GGGCAGCCTC AGCAGGGGCC ACAGGTGACT GGGGCTGGAA CGACAGGTAC AGGGGGCAGC 
TCCAAATCCA AGCCTATCAA CCGGATAAGC ACCGTGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101076483 ( XM_029833498.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029689358.1 Takifugu rubripes IQ motif and SEC7 domain-containing protein 2-like (LOC101076483), transcript variant X3, mRNA.

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CloneID	OTo63425
Clone ID Related Accession (Same CDS sequence)	XM_029833498.1
Accession Version	XM_029833498.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	IQ motif and SEC7 domain-containing protein 2-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042287.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAACA GCACAGAAAG CCCCAACAGG GCGGTGGAGT ATCTCCTGGA GCTCAACAAC 
ATCATCGAGA ACCAGGCCAA GCTCCTGGAG ACCCAGCGCA GACGCATCGA GGAGCTGGAG 
GGCCAGCTGG ACCGCGTCAG CCAGGAGAAC CAGGACCTGA AGCAGGAGCG GAGCCCGCGC 
ACGCCCGGCT CGGACACACC GGAGCAGGAC CACAACCACA GCCAGCCTCT GTCGCTCCCC 
ATCCATCACG GCGGCGGCGC CTCGGCGGCG CAGGTGACGG TCAGCTCGGC CACGACTCCG 
GGGCCCAGCA GGGAGAGGAG GAGCCACACC AGGCTGACCC GGGGGCTCTC CTGCGGCTCG 
TCCACCACCG ACCGAGACCG GCTTGAGCGC ACCGACAGCA CCGACACCAA CAACAGCGCC 
ACCATCCGCC GAAATGTGGA AGGGGAGTCC CAGTTCCCAG ATGTGGGCGC CCGCAAAGAC 
AGCTCCTTCA GCAAGACCCT TTACCGCTCC GAACGCTACG GCGACAGCAG CAGCAGCAGC 
GCACCCTCGG GGTCCAGGGA CCCCTCGTGC GTGGCCCCCT CGAATTCAGC CAGCTTGGCC 
TGGGCGCTCC GAACACGGCA CCAGCCGGCG AGCCTTGCCC TCCGTAAGCA AGAGGAAGAG 
GAGCACAAGA GGTGCAAGGC TCTCTCAGAC AGCTATGAGC TGTCAACAGA TCTGCAGGAT 
AAGAAGGTGG AGATGCTGGA GAGAAAGTAC GGCGGCTCCT TCGTAAGCCG CAGGGCAGCT 
CGAACCATCC AGACGGCGTT CAGGCAGTAC CGCATGAACA AGAACTTTGA ACGCCTCAGG 
AGTTCCGCTT CGGAGAGCCG CATGACTCGG CGCATCATCC TGTCCAACAT GCGACTGCAG 
TACTCCTTTG ACGAGCGGCA GCCGCCACAG CAGGCCCAGA CGCAGACCAA CTTCACCCAC 
AGCGTGGCCA TAGGACCGCC TCACTCCCCC GACACGGACC GTCCGGGAGA CTACACCCAG 
TTAGAGGATT CCTTCTCCAA ACAGGTCAAG TCTTTAGCCG ACTCCATAGA CGATGCTCTC 
ACCTGCCGTG GGGGTCGTAA CGACTCCCAG GAGGGCAGCA GGGAGGGTGG CGGGATTAGC 
GAGGACTTCG GGGAGTGTGT GTGGAGCAGC AGCAGCAACC CCTCCTCCCA AAGAGGATTG 
GGAGAACGCG CCAGGGGGGC AGGAGGAGGA CTCAGCATGC ACGGCGACAG CACCGCCACC 
TCGTACAGCG ACGTCACCCT GTACATGGAT GACGGCATGC CTTCATCGCC CCTGTCCCTG 
GACCGAGCGC CCAGCAGCAC AGACACAGAG TACTGGGGCC CGGGCGGGGG TGTCGGAGGG 
CGCGAGGACA GCAGGGACAC CGAAGGAGGG GGCAGCAGCA ACAGTCGGCG CAGCACCCCG 
TGCACCGAGT GCAGAGACTT CCGTTTTAGA GGCGCCCATC TGCCTCTCCT CACCATCGAG 
CCCCCGAGTG ACAGCTCGGT AGACATGAGC GACCGCTCGG ATCGGGGTTC TTTGAGCAGA 
CAGCTGGTCT ATGAGCAGGA GCCGGGGGTC GGGGTGGGCT CCCCTCAGGG AACCCTCAAA 
CACTCCACCA ACACGGCCAC CCGTGCTTCC TCCACAGCTG CTGCCCAGGG TCAGACTCGG 
GCCCCCGGCA GACCCTTACC CACGCACATC CCGCACCAGG TGGCGGCTCA CCACCACCAT 
CACCACCACC CGGTCCACCA CCATCAGTAC CCCGACACGC CCTCCTCGTC CTCATCGCCC 
CAGCAGCCCC CCACCACCCC TCTGTCCTCC TCCTCGTCCA CCGCTCTGCC TCCCGGTGGG 
CTGGAGCAGC CGTGCTGCTC GGACGGAGAC AACGACTCGC TCAACTCCAC CACTAACTCC 
AACGAGACGG TGAACTGCAG CTCCGGTTCC TCCTCCCAGG ACAGCCTGCA GGAGCCTCTG 
CCACCTCTGG GGAAGCAGAC GTATCAGAGG GAAACGCGCC ACAGCTGGGA CTCGCCGCCC 
TTCAACAGCG ATGTGGTGCA GAGACGCCAG TACCGCATAG GCCTTAACCT CTTCAACAAG 
AAGCCAGAAA AGGGAATCCA GTACTTGATA GAGAGGGGAT TCGTATCAGA CACTCCAGTT 
GGAATTGCTC GTTTCATCCT GGAGAGGAAG GGCCTGAGCC GCCAGATGAT TGGAGAGTTC 
CTGGGAAACC GACAGAAACA GTTCAACAAG GATGTTTTGG ACTGTGTGGT GGATGAAATG 
GACTTCTCAG GTATGGACCT GGATGATGCT CTTCGAAAAT TCCAGGCCCA GATTAAGGTT 
CAAGGAGAAG CCCAGAAAGT GGAGCGGCTC ATAGAGGCTT TTAGTCAGAG ATACTGCGTT 
TGTAATCCGG CTCTGGTTCG CCAGTTCCAA AACCCAGATA CCATCTTCAT CCTGGCCTTT 
GCCATAATCC TGCTGAACAC AGACATGTAC AGTCCCAACG TCAAAGCCGA GCGGAAGATG 
AAACTAGAAG ATTTTATAAA GAATTTAAGG GGTGTGGATA ATGGTCAGGA CATACCCAGG 
GACCTGTTGG TCGGGATCTA CCAGAGAATA CAGAAGTGGG AGCTGCGGAC CAACGACGAT 
CACGTTTCAC AAGTGCTGTC CCTTCCCCAT CGCCGGCTGG TGTGTTGCTG CCAGCTTTAC 
GAAGTTCCGG ACCCCAACCG ACCTCAAAGG ACGGGGGTGC ACCAACGGGA GGTCTTTCTC 
TTTAACGACC TTCTGGTGGT GACCAAAATC TTCCAGAAGA AGAAAACTTC TGTCACCTAC 
AGTTTCAGAC AGTCCTTCTC TTTGGTTGAG ATGCAAGTGC AAATGTTCCA AAACTCATAC 
TACCCTCATG GTATTCGTCT GACCTCGGCG ATCCTGGGCG GCGAGAGGAA GGTTCTCATC 
GTCTTTATGG CGCCCAGTCA ACAAGACCGC ACTCGCTTCG TCAGTGACCT CAGGGAGAGT 
ATTGCTGAAG TTCAAGAGAT GGAGAAATAT AGGGTTGAAT CGGAGCTGGA AAAGCAAAAA 
GGTGTGATGC GGCCCAGTTT GCTGACTGGA GGCGTGGTAG GAGGAGGCGT GGGTGTGAAG 
GGCGACGTGG TGAACGGCAC CCTGGGAAGG ACGAGTTTTG ACGACAACTG TGAGGGGCTG 
AAACGCACCG CGCTCAGCTC CTCTCTCAGG GACCTCTCGG ACACGGGGAA ACATGGTCGC 
AGGAACAGTG TTGGTTCTCT GGATAGTACC ATGGAAGGCT CCATCATTAG CAGTCCCCAG 
CCTCACCAGC GATACCCACT GCCGGGAGTG ATGCCGGGTG TGATGCCAGG TGTGCTGTCG 
GGTGTGATGC CGGGTGTGAT GCCGGGTGTG ATGCCGGGTG TGATGCCGGG TGTGATGCCA 
GGTGTGATGC CGGGTGTTCT CCCAGGTGTG ATCCCGGGAT GCTATGGAAC AGATGACTTC 
CGGTCTCATC GCCCCATCCT GAGCCCCGGG ACGGGGGTGG GGGGTGGGCC GGGGCAGCAA 
AATACCACTG CTGGGACAGA AGTTGGGGGA GTCTCCAGCA GTGTAGAGAG AGCAGGAGCG 
GGGAGCAACC CTGGGGGGAG CAGCAGCAAC AACAGCAGCT CTTTTCTGGG ATCCCTGTTT 
GGCAGCAAAC GTGCCAAACC GCCAGGGCCC CTTATTCCAC CGGGGCCACC CTTCCCCGCA 
CCTGTCCCCC CGCCGACTTC GGGAGGGCCC CCTCCTCACT CCCCTTCATC TCTCTGTCAG 
ATGGACGGGG GACCTTCCAA GATCCAAGCG CTGCATGCGC AGTACTGTCA CACGGGCGCC 
GTGCAGCCTC CACCACCGTA CTACCATCAC CATCGCTTCC ACGTCCAGCC TGTCCAGGCC 
CCTTTGCAAG GCCTACCCCC CCATACTATA CAGAGAGGCC CGCTACCCTG TCGGCCAGTG 
CTTGGCCCCC CACACACCCA GCACCTGCAG CTGGCCCATC TCTCCCAGCT CTCCCAGCCT 
GGCCTTCCGG GTCGCTACGG CAACCTGGTG TTGGGATGTC CTCCCCCTCT TTCTCCTCTC 
TCCCAACATT CCCAGAACCC CCAGTGCGCC CTCTACCACG TTCAGCCCAC ACACTCTATC 
AGAGGGGGCG GCGGTCCCGG TACCAAACTA CCACTAACCG TGTCACACTC CCACCCACAC 
CCCCACGCAC ACTCCCAAAC CCACCCCGGA CACGTGCACA CGCCCCACCC AGCCAGCCAA 
TCCACGCACC AAACACACTT TATATTCGGC ACTCTGCCCC ACAACCTTCC GTCCGCCTCC 
GTCAATGCGC ATCACGCAGC CTCCGCCGCC CCGTATCTGC CCCAGTACCC TCCTCTCTCC 
TCCATCCCAC CTCCCCCACC GCACTCCCCT TTACCCCCCC CTTCGTCCCC CCTTACCCCT 
CTTACCCCTC TCTCCCCTCA CCCCCCCCAG CACCCCGGGC AGCCTCAGCA GGGGCCACAG 
GTGACTGGGG CTGGAACGAC AGGTACAGGG GGCAGCTCCA AATCCAAGCC TATCAACCGG 
ATAAGCACCG TGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029689358.1
CDS	1214..5791
Translation	MENSTESPNRAVEYLLELNNIIENQAKLLETQRRRIEELEGQLDRVSQENQDLKQERSPRTPGSDTPEQDHNHSQPLSLPIHHGGGASAAQVTVSSATTPGPSRERRSHTRLTRGLSCGSSTTDRDRLERTDSTDTNNSATIRRNVEGESQFPDVGARKDSSFSKTLYRSERYGDSSSSSAPSGSRDPSCVAPSNSASLAWALRTRHQPASLALRKQEEEEHKRCKALSDSYELSTDLQDKKVEMLERKYGGSFVSRRAARTIQTAFRQYRMNKNFERLRSSASESRMTRRIILSNMRLQYSFDERQPPQQAQTQTNFTHSVAIGPPHSPDTDRPGDYTQLEDSFSKQVKSLADSIDDALTCRGGRNDSQEGSREGGGISEDFGECVWSSSSNPSSQRGLGERARGAGGGLSMHGDSTATSYSDVTLYMDDGMPSSPLSLDRAPSSTDTEYWGPGGGVGGREDSRDTEGGGSSNSRRSTPCTECRDFRFRGAHLPLLTIEPPSDSSVDMSDRSDRGSLSRQLVYEQEPGVGVGSPQGTLKHSTNTATRASSTAAAQGQTRAPGRPLPTHIPHQVAAHHHHHHHPVHHHQYPDTPSSSSSPQQPPTTPLSSSSSTALPPGGLEQPCCSDGDNDSLNSTTNSNETVNCSSGSSSQDSLQEPLPPLGKQTYQRETRHSWDSPPFNSDVVQRRQYRIGLNLFNKKPEKGIQYLIERGFVSDTPVGIARFILERKGLSRQMIGEFLGNRQKQFNKDVLDCVVDEMDFSGMDLDDALRKFQAQIKVQGEAQKVERLIEAFSQRYCVCNPALVRQFQNPDTIFILAFAIILLNTDMYSPNVKAERKMKLEDFIKNLRGVDNGQDIPRDLLVGIYQRIQKWELRTNDDHVSQVLSLPHRRLVCCCQLYEVPDPNRPQRTGVHQREVFLFNDLLVVTKIFQKKKTSVTYSFRQSFSLVEMQVQMFQNSYYPHGIRLTSAILGGERKVLIVFMAPSQQDRTRFVSDLRESIAEVQEMEKYRVESELEKQKGVMRPSLLTGGVVGGGVGVKGDVVNGTLGRTSFDDNCEGLKRTALSSSLRDLSDTGKHGRRNSVGSLDSTMEGSIISSPQPHQRYPLPGVMPGVMPGVLSGVMPGVMPGVMPGVMPGVMPGVMPGVLPGVIPGCYGTDDFRSHRPILSPGTGVGGGPGQQNTTAGTEVGGVSSSVERAGAGSNPGGSSSNNSSSFLGSLFGSKRAKPPGPLIPPGPPFPAPVPPPTSGGPPPHSPSSLCQMDGGPSKIQALHAQYCHTGAVQPPPPYYHHHRFHVQPVQAPLQGLPPHTIQRGPLPCRPVLGPPHTQHLQLAHLSQLSQPGLPGRYGNLVLGCPPPLSPLSQHSQNPQCALYHVQPTHSIRGGGGPGTKLPLTVSHSHPHPHAHSQTHPGHVHTPHPASQSTHQTHFIFGTLPHNLPSASVNAHHAASAAPYLPQYPPLSSIPPPPPHSPLPPPSSPLTPLTPLSPHPPQHPGQPQQGPQVTGAGTTGTGGSSKSKPINRISTVV

Target ORF information:

RefSeq Version	XM_029833498.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes IQ motif and SEC7 domain-containing protein 2-like (LOC101076483), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029833498.1

ORF Insert Sequence:

ATGGAGAACA GCACAGAAAG CCCCAACAGG GCGGTGGAGT ATCTCCTGGA GCTCAACAAC 
ATCATCGAGA ACCAGGCCAA GCTCCTGGAG ACCCAGCGCA GACGCATCGA GGAGCTGGAG 
GGCCAGCTGG ACCGCGTCAG CCAGGAGAAC CAGGACCTGA AGCAGGAGCG GAGCCCGCGC 
ACGCCCGGCT CGGACACACC GGAGCAGGAC CACAACCACA GCCAGCCTCT GTCGCTCCCC 
ATCCATCACG GCGGCGGCGC CTCGGCGGCG CAGGTGACGG TCAGCTCGGC CACGACTCCG 
GGGCCCAGCA GGGAGAGGAG GAGCCACACC AGGCTGACCC GGGGGCTCTC CTGCGGCTCG 
TCCACCACCG ACCGAGACCG GCTTGAGCGC ACCGACAGCA CCGACACCAA CAACAGCGCC 
ACCATCCGCC GAAATGTGGA AGGGGAGTCC CAGTTCCCAG ATGTGGGCGC CCGCAAAGAC 
AGCTCCTTCA GCAAGACCCT TTACCGCTCC GAACGCTACG GCGACAGCAG CAGCAGCAGC 
GCACCCTCGG GGTCCAGGGA CCCCTCGTGC GTGGCCCCCT CGAATTCAGC CAGCTTGGCC 
TGGGCGCTCC GAACACGGCA CCAGCCGGCG AGCCTTGCCC TCCGTAAGCA AGAGGAAGAG 
GAGCACAAGA GGTGCAAGGC TCTCTCAGAC AGCTATGAGC TGTCAACAGA TCTGCAGGAT 
AAGAAGGTGG AGATGCTGGA GAGAAAGTAC GGCGGCTCCT TCGTAAGCCG CAGGGCAGCT 
CGAACCATCC AGACGGCGTT CAGGCAGTAC CGCATGAACA AGAACTTTGA ACGCCTCAGG 
AGTTCCGCTT CGGAGAGCCG CATGACTCGG CGCATCATCC TGTCCAACAT GCGACTGCAG 
TACTCCTTTG ACGAGCGGCA GCCGCCACAG CAGGCCCAGA CGCAGACCAA CTTCACCCAC 
AGCGTGGCCA TAGGACCGCC TCACTCCCCC GACACGGACC GTCCGGGAGA CTACACCCAG 
TTAGAGGATT CCTTCTCCAA ACAGGTCAAG TCTTTAGCCG ACTCCATAGA CGATGCTCTC 
ACCTGCCGTG GGGGTCGTAA CGACTCCCAG GAGGGCAGCA GGGAGGGTGG CGGGATTAGC 
GAGGACTTCG GGGAGTGTGT GTGGAGCAGC AGCAGCAACC CCTCCTCCCA AAGAGGATTG 
GGAGAACGCG CCAGGGGGGC AGGAGGAGGA CTCAGCATGC ACGGCGACAG CACCGCCACC 
TCGTACAGCG ACGTCACCCT GTACATGGAT GACGGCATGC CTTCATCGCC CCTGTCCCTG 
GACCGAGCGC CCAGCAGCAC AGACACAGAG TACTGGGGCC CGGGCGGGGG TGTCGGAGGG 
CGCGAGGACA GCAGGGACAC CGAAGGAGGG GGCAGCAGCA ACAGTCGGCG CAGCACCCCG 
TGCACCGAGT GCAGAGACTT CCGTTTTAGA GGCGCCCATC TGCCTCTCCT CACCATCGAG 
CCCCCGAGTG ACAGCTCGGT AGACATGAGC GACCGCTCGG ATCGGGGTTC TTTGAGCAGA 
CAGCTGGTCT ATGAGCAGGA GCCGGGGGTC GGGGTGGGCT CCCCTCAGGG AACCCTCAAA 
CACTCCACCA ACACGGCCAC CCGTGCTTCC TCCACAGCTG CTGCCCAGGG TCAGACTCGG 
GCCCCCGGCA GACCCTTACC CACGCACATC CCGCACCAGG TGGCGGCTCA CCACCACCAT 
CACCACCACC CGGTCCACCA CCATCAGTAC CCCGACACGC CCTCCTCGTC CTCATCGCCC 
CAGCAGCCCC CCACCACCCC TCTGTCCTCC TCCTCGTCCA CCGCTCTGCC TCCCGGTGGG 
CTGGAGCAGC CGTGCTGCTC GGACGGAGAC AACGACTCGC TCAACTCCAC CACTAACTCC 
AACGAGACGG TGAACTGCAG CTCCGGTTCC TCCTCCCAGG ACAGCCTGCA GGAGCCTCTG 
CCACCTCTGG GGAAGCAGAC GTATCAGAGG GAAACGCGCC ACAGCTGGGA CTCGCCGCCC 
TTCAACAGCG ATGTGGTGCA GAGACGCCAG TACCGCATAG GCCTTAACCT CTTCAACAAG 
AAGCCAGAAA AGGGAATCCA GTACTTGATA GAGAGGGGAT TCGTATCAGA CACTCCAGTT 
GGAATTGCTC GTTTCATCCT GGAGAGGAAG GGCCTGAGCC GCCAGATGAT TGGAGAGTTC 
CTGGGAAACC GACAGAAACA GTTCAACAAG GATGTTTTGG ACTGTGTGGT GGATGAAATG 
GACTTCTCAG GTATGGACCT GGATGATGCT CTTCGAAAAT TCCAGGCCCA GATTAAGGTT 
CAAGGAGAAG CCCAGAAAGT GGAGCGGCTC ATAGAGGCTT TTAGTCAGAG ATACTGCGTT 
TGTAATCCGG CTCTGGTTCG CCAGTTCCAA AACCCAGATA CCATCTTCAT CCTGGCCTTT 
GCCATAATCC TGCTGAACAC AGACATGTAC AGTCCCAACG TCAAAGCCGA GCGGAAGATG 
AAACTAGAAG ATTTTATAAA GAATTTAAGG GGTGTGGATA ATGGTCAGGA CATACCCAGG 
GACCTGTTGG TCGGGATCTA CCAGAGAATA CAGAAGTGGG AGCTGCGGAC CAACGACGAT 
CACGTTTCAC AAGTGCTGTC CCTTCCCCAT CGCCGGCTGG TGTGTTGCTG CCAGCTTTAC 
GAAGTTCCGG ACCCCAACCG ACCTCAAAGG ACGGGGGTGC ACCAACGGGA GGTCTTTCTC 
TTTAACGACC TTCTGGTGGT GACCAAAATC TTCCAGAAGA AGAAAACTTC TGTCACCTAC 
AGTTTCAGAC AGTCCTTCTC TTTGGTTGAG ATGCAAGTGC AAATGTTCCA AAACTCATAC 
TACCCTCATG GTATTCGTCT GACCTCGGCG ATCCTGGGCG GCGAGAGGAA GGTTCTCATC 
GTCTTTATGG CGCCCAGTCA ACAAGACCGC ACTCGCTTCG TCAGTGACCT CAGGGAGAGT 
ATTGCTGAAG TTCAAGAGAT GGAGAAATAT AGGGTTGAAT CGGAGCTGGA AAAGCAAAAA 
GGTGTGATGC GGCCCAGTTT GCTGACTGGA GGCGTGGTAG GAGGAGGCGT GGGTGTGAAG 
GGCGACGTGG TGAACGGCAC CCTGGGAAGG ACGAGTTTTG ACGACAACTG TGAGGGGCTG 
AAACGCACCG CGCTCAGCTC CTCTCTCAGG GACCTCTCGG ACACGGGGAA ACATGGTCGC 
AGGAACAGTG TTGGTTCTCT GGATAGTACC ATGGAAGGCT CCATCATTAG CAGTCCCCAG 
CCTCACCAGC GATACCCACT GCCGGGAGTG ATGCCGGGTG TGATGCCAGG TGTGCTGTCG 
GGTGTGATGC CGGGTGTGAT GCCGGGTGTG ATGCCGGGTG TGATGCCGGG TGTGATGCCA 
GGTGTGATGC CGGGTGTTCT CCCAGGTGTG ATCCCGGGAT GCTATGGAAC AGATGACTTC 
CGGTCTCATC GCCCCATCCT GAGCCCCGGG ACGGGGGTGG GGGGTGGGCC GGGGCAGCAA 
AATACCACTG CTGGGACAGA AGTTGGGGGA GTCTCCAGCA GTGTAGAGAG AGCAGGAGCG 
GGGAGCAACC CTGGGGGGAG CAGCAGCAAC AACAGCAGCT CTTTTCTGGG ATCCCTGTTT 
GGCAGCAAAC GTGCCAAACC GCCAGGGCCC CTTATTCCAC CGGGGCCACC CTTCCCCGCA 
CCTGTCCCCC CGCCGACTTC GGGAGGGCCC CCTCCTCACT CCCCTTCATC TCTCTGTCAG 
ATGGACGGGG GACCTTCCAA GATCCAAGCG CTGCATGCGC AGTACTGTCA CACGGGCGCC 
GTGCAGCCTC CACCACCGTA CTACCATCAC CATCGCTTCC ACGTCCAGCC TGTCCAGGCC 
CCTTTGCAAG GCCTACCCCC CCATACTATA CAGAGAGGCC CGCTACCCTG TCGGCCAGTG 
CTTGGCCCCC CACACACCCA GCACCTGCAG CTGGCCCATC TCTCCCAGCT CTCCCAGCCT 
GGCCTTCCGG GTCGCTACGG CAACCTGGTG TTGGGATGTC CTCCCCCTCT TTCTCCTCTC 
TCCCAACATT CCCAGAACCC CCAGTGCGCC CTCTACCACG TTCAGCCCAC ACACTCTATC 
AGAGGGGGCG GCGGTCCCGG TACCAAACTA CCACTAACCG TGTCACACTC CCACCCACAC 
CCCCACGCAC ACTCCCAAAC CCACCCCGGA CACGTGCACA CGCCCCACCC AGCCAGCCAA 
TCCACGCACC AAACACACTT TATATTCGGC ACTCTGCCCC ACAACCTTCC GTCCGCCTCC 
GTCAATGCGC ATCACGCAGC CTCCGCCGCC CCGTATCTGC CCCAGTACCC TCCTCTCTCC 
TCCATCCCAC CTCCCCCACC GCACTCCCCT TTACCCCCCC CTTCGTCCCC CCTTACCCCT 
CTTACCCCTC TCTCCCCTCA CCCCCCCCAG CACCCCGGGC AGCCTCAGCA GGGGCCACAG 
GTGACTGGGG CTGGAACGAC AGGTACAGGG GGCAGCTCCA AATCCAAGCC TATCAACCGG 
ATAAGCACCG TGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC101076483 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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