atg2b cDNA ORF clone, Takifugu rubripes(Fugu rubripes)

Gene
Clones
gRNAs

Summary

Gene Symbol	atg2b
Entrez Gene ID	101069427
Full Name	autophagy related 2B
Gene Type	protein-coding
Organism	Takifugu rubripes(Fugu rubripes)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011617041.2	XP_011615343.2	autophagy-related protein 2 homolog B isoform X1
XM_011617055.1	XP_011615357.1	autophagy-related protein 2 homolog B isoform X3
XM_011617068.2	XP_011615370.2	autophagy-related protein 2 homolog B isoform X2
XM_029832640.1	XP_029688500.1	autophagy-related protein 2 homolog B isoform X1
XM_011617061.1	XP_011615363.1	autophagy-related protein 2 homolog B isoform X4
XM_011617049.1	XP_011615351.1	autophagy-related protein 2 homolog B isoform X2
XM_011617068.1	XP_011615370.1	autophagy-related protein 2 homolog B isoform X5
XM_011617041.1	XP_011615343.1	autophagy-related protein 2 homolog B isoform X1

Pathways from BioSystems
Gene Ontology

The following atg2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the atg2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTo24750	XM_011617068.1 Latest version!	Takifugu rubripes autophagy related 2B (atg2b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OTo73021	XM_011617068.2 Latest version!	Takifugu rubripes autophagy related 2B (atg2b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OTo24747	XM_011617041.1 Latest version!	Takifugu rubripes autophagy related 2B (atg2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OTo24748	XM_011617055.1 Latest version!	Takifugu rubripes autophagy related 2B (atg2b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OTo24748	XM_011617049.1 Latest version!	Takifugu rubripes autophagy related 2B (atg2b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OTo24749	XM_011617061.1 Latest version!	Takifugu rubripes autophagy related 2B (atg2b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OTo73020	XM_029832640.1 Latest version!	Takifugu rubripes autophagy related 2B (atg2b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OTo73020	XM_011617041.2 Latest version!	Takifugu rubripes autophagy related 2B (atg2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

atg2b ( XM_011617068.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011615370.1 Takifugu rubripes autophagy related 2B (atg2b), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OTo24750
Clone ID Related Accession (Same CDS sequence)	XM_011617068.1
Accession Version	XM_011617068.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5877bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426348800000
Organism	Takifugu rubripes(Fugu rubripes)
Product	autophagy-related protein 2 homolog B isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070921.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCTGGT CCAGTTTTAT GACAAGCAGC ATGCAACTGG CCAAAGAATG CCTAAGCCAG 
AAGCTCACGG ATGACCTCGG AGAGAGTTTT CAGCCTTTTG AAGGATTAGA GAAGTTTGCC 
GAAACAATAG AAACAGTCCT GAGGAGAGTT AAAGTGACAT TTTTGGATAC AGTTCTCAGA 
ATTGAACACA TTCCAGAAAA TTCTAAAACT GGAATCGCGC TGGAGATTCG AATTAACAAG 
ACGGTTTACT GTGATGAAAC TAACGAGGAG ACTTCAGACG TTAACGTGCA TCAGCCGACC 
ACGTTTGCCC ACAAAAACCT GCAGATGGAA GGCATCACTG TGTTCTGGGA CGAGTTCTCC 
GAGGTGTCCC GCGCTGGCTG TAAATCTTCA CCAACTCCCA CGGAAACCGA ACCAAAACTG 
TCCCCGAGCT GGAATCCTAA AATAATCTGC GAGCCGCATC CCCAGTTCAC AGAACCAGTA 
TACTCTGCAG CACCATTTGA AGCTGTGCAG GTCGGGAGCC TTGGTGGGAA AATCAAGATC 
TCCCTCACTC TGAAGAACAA CCTAGCTATG CCCGGAGCAA AGCTGGATGT TGACGGTCAC 
ATCGACACAC TGTTGATTCT GCTGTCGCCG CGACAAGTTC ATCTTCTTTT GGACCTATTC 
GGAGCCTTTT CCGGTGGAGG TTCACAAGAA TGGAGCAAGG ACAGAAAGAG CAGACCCATG 
CAACAGGAGG ACGAGTACCG GCTCCACATG GAGCTGAACC GGTGTCTAAA GAAAGACACC 
ACGGTCACAG GAGTCGACCC TGAATTGTTT GAAAGCCAAA CCACAAGAAC CGTGTCGAGT 
CGAGACGACG TGTTCTTTTC CATGGCCGAC ATGGATATGT CTCACAGCCT GTCGTCGCTG 
CCTCCTCTTG GTGAACCGCC CACAGTCGAC TTGGATTTAT CTCTTAACAG CAACTACTCT 
GCTTCTCCTG GAGAATCTCC CTCTGCTAAT GCAACAGCTC TGTGGGATGA TTACATTGAT 
CTACCTCGAC ACAGAGAGAA GCAGGTTAAT GAAAGTCCAA TTGTATTGCA AGACTCTCAA 
CTTCATCAAA ATGTATTGAG ACAAGCGTCC CACCCTTCCA AACCTCATGG TGATGAGTCT 
CGACCAGAGC TGCTTTTGAA TCTGAAGCTG AGCAGCCTGG CCATCTCGGT CCTCCACATC 
GACCCGTTGC CACCACCGGA TGCAGCTCAC AGTCCCCTCG GTCCTATGGC TGCACACTTC 
TTCCGCACAG TAGGGCCCCA GCATCTTGCC CCAGCGGCTT TCCTTGAGTC TCGAAACATT 
TTTAACCAGG CCTGCCCCCA TGACAATCTC AGGTTTGTGG CCCAGGGGCT CAAGATCAGC 
TATGAGCACT GTAAGGGATC CAGTCTGCGG ACCTTCAACA CCGACGTCTC TCTCAACCAG 
ATGGAGTTTT TAGAGTGCCT GTTTCCATCT GAGGGTTTCA GCAGCGACTC CCAAAGAGGC 
ATTCAGTACA CAGAGCTCCT GACATTTGAC ATGGCGAGCC CTGATGTGCC TCTGCAGACC 
TGCCTTCACC TGCTGTACAA ACAGAAAGAG AGGAAAGGCC CTCAGAGCGG TCAGTTTCGA 
CTCAGCACCA TTCCCAGGAA AGCAGAAATC CAGGTGGAGC TGGGCCCGGT GCGATGCGAG 
CTGGACATTA GTATTGTAGA CCGTCTCAAC TCGTTGCTCC AACCCCAGAA GCTGGCCGCC 
ACAGAGCTGA TGGCGTCTCA CCTGTACACA TCCTATAATA AACACGTCAG CCTGCATAAA 
GCCTTTACAG AGGTTTTTCT GGATGACAGT CACACTCCCA GCAACTGTCA CGTGTCATTG 
ACTGTAAACG CGCCGGTGCT GGGCCTCGCT CTCCGCTTCC CTATTCCTGA CCTGCGCTCA 
GATCAGGAAC GGGGCCCCTG GTTTAAGAAG TCCCTGCAGA AGGAAGTCCT CTTTCTGGAA 
CTGGAAGATC TGGAAGTCAA AACAGAATTC ATGGCTGGCA ACTCACCTGA TCAAACTAAG 
TTGGAGCTCA CATTCAGGGA GCTCATTGGA AAATTTCAAC AGGAGACTAA CCACCCAGCC 
GTCCGCTTCC TCCGAGTGTC TCACACCATG GACGGAGACA TGACAACGTC AGAAAGTGTT 
AAATTTGACT GGCCGAGGGT CGTGCTGAAG ATGAACCCCA CTGCAGTGCA CTCGATACTT 
GAGCGGGTGC AGGCCGAAGA TGATGAGGGG GTAGAGGACC ATTCACTCGA AGAGGAAGAG 
GAGGAGGGCG CTGCACATTC ACTGAAGGAC GTGTGTGACT TTGGTAAAGC AGAGCCATCT 
CCGTTTTCAT CGCGAAGGGT CATGTACGAA AATGAGGAGA TGGTCATTCC GGGTGACGTC 
ACTGAGATGA CGGAATTCCA GGAGAAGACC ATGAGCAACT CAAGGTTCAT TGTTGAGTTG 
TGTCTCCCCA ATGTGCAGTT ATCCTTCCCC AGCAAAGCAT TTTATGAAAC ACTGCACAAC 
AGGATAAATA ACGATTTGCT GCTTTGGGAG CCGACTGCTC CATCTCCCGT GGAGACGGTG 
GAAAACATCC CTTATGGAGT TGGCCTCTCT GTCGCCAGTC AGTTGATCAA CACTTACTCC 
AAAGACAGCT TTAGTCAGTT TCGCTCCTCT GGTCCTGATG ATGAGACCAG CGGGTCAGAT 
GAAGAAACCA TGCATTATTA CATTCCTGCT TCAGACCCAA GCCTCCAGTC TCGCAAGAAG 
AAAAAGCCCA AAGCCCAAAG CAAGACCTCC CAGAGCCTAT TTTCTGTCAT CGTCAGTGTC 
AATCACGGTC TGATCGCGCT ACAAGCTAAT GCTATGAAGG ACAAAACTGT ATTGAAAAAC 
AAACATGGTG AATTCTGGCT GGAGCTAAAG AATGGCGTGT TGTTCAGTGT CATGCAGCAC 
GAGGGCTACA AAGATCAACA TTACATCTGT TTCCACACCA GCAGTGTTTG CATGTATCAC 
CAAGGACTTG TGGATGGAGG CCTTTCCGTC TTGGACGTCA ATTTGCCGTC CAGGACACAT 
CCTCACTGGT TAGATCCAAC TATCTACCAA TCAGAGACTT CCCCCGAGAG ATCTTCAACG 
CCCTCAGAGT GCATCGGCCT GGAGGCTCGG AGCATGGTGT CCGTGGCCGT CAAAGTATCA 
TCACCGAACC CAGAGCACAA CATCAAGGAA ATTCTTATTG CTGTTGGAGT GAGAGGAGCC 
ACCTTGCAGC ATAAAATGGC CCCTCCTTGC ACGGGTTGGT ACGATCAGAT TGTTGACTTT 
CTGAATATCA CCAATGAGCC AGTGTTGGGA TACGCCCCTC CCACCTCGGT CTCCACCCTA 
CACTTACACC TGTGGAGCTG TTCTCTTGAC TACAGACCCC TTTATCTGCC ACTCAGATCA 
CTGTTGGTTG TAGAGACCTT TAGTATCTCC AGCAGTGTGT CTTTAGATCA CTCTTCCTCA 
TCACTCAGGA TCATATTGGA TGAGGCTGCC GTGTTCCTGT CAGACAGGGC CAATGCCGTC 
TCTGTCAATC TGGCACGTGA CTATGTGCAA GTGGTGGACA TGGGAACTCT GGAGCTCAGG 
ATCACAGCTG CCAAACCTGG AGCTGACGGA AAGCTGAAAG AGCCAAGGTT CGAACTGCGC 
TGCTCCAGCG ACATTATTCA CATCAGAACC TGTTCGGACT CCTGTGCTGC CCTCATGAAC 
CTTATTCAGT ATGTAGCCAG CTATGGAGAC TTACTGCCCC CAGCAGAACC AGAGCCCAAA 
CACAGCAGCA CCTCACAGAG GACCAAGGTT GAATTTCCAA GTCGGGTCAC ACCTCAGACG 
TCGTTTCTGG CTGATATTGA GCAGCAGATG TTGCAGGATT TGATGAGCGA AGCGATGGAG 
GAGACAGAAG GCCAGCATGC ATCAGGGCTG CAGCAGAATG GCGCACGAGA AGAACACTGT 
CAAGATCACG ATCCACCCCG CTCAGACCTG TTCTTGTTCC CGGATGAGAG CGGAAATCTT 
AACCAAGATT TAAGCCCCAC TTGTCCCGTC CTTCACTCTC CTCTCATCAC CCCTGTTCCC 
AATCTGGCCC AAGAGGCTGA TGACTTTTGT ATCCTGGAGA CACCCGGCTT CAGACGAGAG 
GATCTTGATC AGGAACCAGT GGTAAAGCGG CTTACTTCAG ATCCTGTGGT CATTAAAGAT 
GACCATTTCA GCCAGCCCTT GTGTGGGAGT GACTCAAGCC ACGGAGCCCT GAACTTTCCC 
ATCCCAGAGG TGCGCTACCT CATCAAGGAG ATCTCTGTCA TCTGGCACCT CTACGGTGGG 
AAAGACTTTG GGAGCACAAC GTTAACAGCA TCTCCTTCTA GAAGTTGGGG TTCTACACCT 
CATAGCTCCC CCTCCCAGAC TCCGGTCCGA CAATCCAAGA GTTCAAGCCG TTCTGGGGGA 
GGGAGAGGAA GGAACACTGA TGTGTTGATG GAGATACAGC TCAGCAAGGT GCGGTTTCAG 
CACGATGTGT ACCCGCAGGC TCAGGGGGCT TCTGGGCCGA CAGTGGATCA GCCGGTGTCT 
CGACAGGTGT TTGTGGTCCA GGACTTGGAG ATTCGAGACA GACTAGCTGC CTCACAGATG 
AATAAGTTCC TTTACTTGTA TTCGAGCAAG GAAATGCCCA GAAAAGCTCA TTCAAATATG 
TTGACAGTTA AGGCTCTCCA CATGTGTCCG GAGTCCAGCC AGGCTCCACA GGAATGCTGC 
TTGCGTGTTT CACTCACGCC CCTTCGCCTC AATATTGATC AGGATGCACT GTTTTTCTTG 
AAGGACTTCT TTACCAGTCT TGCTACAGAG GTGGAGTTTT TCTCATCGCC ATCACAAGAA 
GCCATCTGTG TTCCTATTAA AAAAGCAGCC ACCCCAGAGA TCTCTTGTAG CTTCTCCAAA 
CACGCCAGCA GCAGCCACGA CCCAGCCCCA ATCATCTCGC TGCCCACTCA GAGAGGTTTG 
AATCACAATG GCTTCTCCAG CAGGGAGGAA ATGGCTGACA GTGAATCTTC TGCACCCTCG 
TTCACAGACC AGCCCATATT CTTTAGGGAG TTCAGATTTA CCTCTGAGGT TCCAATTCGT 
CTGGATTACC ATGGAAAACA CGTGTCGATG GAGCAGGGGA CCTTTGCTGG AATAATTATG 
GGGTTGACAC AACTTAACTG CTCAGAGTTG AAATTGAGGC GCTTGTGCTA CAGACAAGGA 
TTATTAGGTG TGGATAAGTT GTTTTCCTAT GCAATCAACG AATGGCTTAC TGACATAAAG 
AAGAATCAGC TTCCGGGGCT GTTGGGTGGT GTGGGGCCTA TTCACTCACT GGTCCAGCTG 
GTTCAGGGAT TCAGGGATTT GGTCTGGCTC CCCATCGAGC AGTACAGGAA AGACGGTCGG 
ATTGTTCGTG GTTTTCAGAG GGGCACTGCC TCTTTCGGCA CGTCGACAGC CATGGCCGCC 
CTGGAGCTCA CCAACAGGAT GGTGCGGACC ATCCAGGCAG CAGCAGAGAC AGCCTACGAC 
ATGGTGTCTC CAGTCCCTGA TGAGAGAGAC ACAAAGAGGG TAAAACGGTA CATCCCTTAC 
GGACTGGCTC ATCAGCCCGT CGACCTGAGA GAGGGCGTGG CCAAAGCTTA CACAGTAGTA 
AAAGAGGGCA TTACAGACAC GGCACTGACC ATCTATGACA CAGCCACCAG GGAGCACGAA 
CAGCGCGGGA TGACAGGGGC AGTGGGCGGA GTTCTCCGCC AGCTGCCGCC AGCTGTGGTC 
AAGCCGCTCA TCGTGGCCAC TGAGGCCACC TCTAATGTCT TAGGTGGAAT GAGGAACCAG 
ATTCACCCTG ACGCAAGGCA GGAAGAGTCA CAGAAGTGGA GGCAGGGCGA AGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011615370.1
CDS	75..5951
Translation	MCWSSFMTSSMQLAKECLSQKLTDDLGESFQPFEGLEKFAETIETVLRRVKVTFLDTVLRIEHIPENSKTGIALEIRINKTVYCDETNEETSDVNVHQPTTFAHKNLQMEGITVFWDEFSEVSRAGCKSSPTPTETEPKLSPSWNPKIICEPHPQFTEPVYSAAPFEAVQVGSLGGKIKISLTLKNNLAMPGAKLDVDGHIDTLLILLSPRQVHLLLDLFGAFSGGGSQEWSKDRKSRPMQQEDEYRLHMELNRCLKKDTTVTGVDPELFESQTTRTVSSRDDVFFSMADMDMSHSLSSLPPLGEPPTVDLDLSLNSNYSASPGESPSANATALWDDYIDLPRHREKQVNESPIVLQDSQLHQNVLRQASHPSKPHGDESRPELLLNLKLSSLAISVLHIDPLPPPDAAHSPLGPMAAHFFRTVGPQHLAPAAFLESRNIFNQACPHDNLRFVAQGLKISYEHCKGSSLRTFNTDVSLNQMEFLECLFPSEGFSSDSQRGIQYTELLTFDMASPDVPLQTCLHLLYKQKERKGPQSGQFRLSTIPRKAEIQVELGPVRCELDISIVDRLNSLLQPQKLAATELMASHLYTSYNKHVSLHKAFTEVFLDDSHTPSNCHVSLTVNAPVLGLALRFPIPDLRSDQERGPWFKKSLQKEVLFLELEDLEVKTEFMAGNSPDQTKLELTFRELIGKFQQETNHPAVRFLRVSHTMDGDMTTSESVKFDWPRVVLKMNPTAVHSILERVQAEDDEGVEDHSLEEEEEEGAAHSLKDVCDFGKAEPSPFSSRRVMYENEEMVIPGDVTEMTEFQEKTMSNSRFIVELCLPNVQLSFPSKAFYETLHNRINNDLLLWEPTAPSPVETVENIPYGVGLSVASQLINTYSKDSFSQFRSSGPDDETSGSDEETMHYYIPASDPSLQSRKKKKPKAQSKTSQSLFSVIVSVNHGLIALQANAMKDKTVLKNKHGEFWLELKNGVLFSVMQHEGYKDQHYICFHTSSVCMYHQGLVDGGLSVLDVNLPSRTHPHWLDPTIYQSETSPERSSTPSECIGLEARSMVSVAVKVSSPNPEHNIKEILIAVGVRGATLQHKMAPPCTGWYDQIVDFLNITNEPVLGYAPPTSVSTLHLHLWSCSLDYRPLYLPLRSLLVVETFSISSSVSLDHSSSSLRIILDEAAVFLSDRANAVSVNLARDYVQVVDMGTLELRITAAKPGADGKLKEPRFELRCSSDIIHIRTCSDSCAALMNLIQYVASYGDLLPPAEPEPKHSSTSQRTKVEFPSRVTPQTSFLADIEQQMLQDLMSEAMEETEGQHASGLQQNGAREEHCQDHDPPRSDLFLFPDESGNLNQDLSPTCPVLHSPLITPVPNLAQEADDFCILETPGFRREDLDQEPVVKRLTSDPVVIKDDHFSQPLCGSDSSHGALNFPIPEVRYLIKEISVIWHLYGGKDFGSTTLTASPSRSWGSTPHSSPSQTPVRQSKSSSRSGGGRGRNTDVLMEIQLSKVRFQHDVYPQAQGASGPTVDQPVSRQVFVVQDLEIRDRLAASQMNKFLYLYSSKEMPRKAHSNMLTVKALHMCPESSQAPQECCLRVSLTPLRLNIDQDALFFLKDFFTSLATEVEFFSSPSQEAICVPIKKAATPEISCSFSKHASSSHDPAPIISLPTQRGLNHNGFSSREEMADSESSAPSFTDQPIFFREFRFTSEVPIRLDYHGKHVSMEQGTFAGIIMGLTQLNCSELKLRRLCYRQGLLGVDKLFSYAINEWLTDIKKNQLPGLLGGVGPIHSLVQLVQGFRDLVWLPIEQYRKDGRIVRGFQRGTASFGTSTAMAALELTNRMVRTIQAAAETAYDMVSPVPDERDTKRVKRYIPYGLAHQPVDLREGVAKAYTVVKEGITDTALTIYDTATREHEQRGMTGAVGGVLRQLPPAVVKPLIVATEATSNVLGGMRNQIHPDARQEESQKWRQGEE

Target ORF information:

RefSeq Version	XM_011617068.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes autophagy related 2B (atg2b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011617068.1

ORF Insert Sequence:

ATGTGCTGGT CCAGTTTTAT GACAAGCAGC ATGCAACTGG CCAAAGAATG CCTAAGCCAG 
AAGCTCACGG ATGACCTCGG AGAGAGTTTT CAGCCTTTTG AAGGATTAGA GAAGTTTGCC 
GAAACAATAG AAACAGTCCT GAGGAGAGTT AAAGTGACAT TTTTGGATAC AGTTCTCAGA 
ATTGAACACA TTCCAGAAAA TTCTAAAACT GGAATCGCGC TGGAGATTCG AATTAACAAG 
ACGGTTTACT GTGATGAAAC TAACGAGGAG ACTTCAGACG TTAACGTGCA TCAGCCGACC 
ACGTTTGCCC ACAAAAACCT GCAGATGGAA GGCATCACTG TGTTCTGGGA CGAGTTCTCC 
GAGGTGTCCC GCGCTGGCTG TAAATCTTCA CCAACTCCCA CGGAAACCGA ACCAAAACTG 
TCCCCGAGCT GGAATCCTAA AATAATCTGC GAGCCGCATC CCCAGTTCAC AGAACCAGTA 
TACTCTGCAG CACCATTTGA AGCTGTGCAG GTCGGGAGCC TTGGTGGGAA AATCAAGATC 
TCCCTCACTC TGAAGAACAA CCTAGCTATG CCCGGAGCAA AGCTGGATGT TGACGGTCAC 
ATCGACACAC TGTTGATTCT GCTGTCGCCG CGACAAGTTC ATCTTCTTTT GGACCTATTC 
GGAGCCTTTT CCGGTGGAGG TTCACAAGAA TGGAGCAAGG ACAGAAAGAG CAGACCCATG 
CAACAGGAGG ACGAGTACCG GCTCCACATG GAGCTGAACC GGTGTCTAAA GAAAGACACC 
ACGGTCACAG GAGTCGACCC TGAATTGTTT GAAAGCCAAA CCACAAGAAC CGTGTCGAGT 
CGAGACGACG TGTTCTTTTC CATGGCCGAC ATGGATATGT CTCACAGCCT GTCGTCGCTG 
CCTCCTCTTG GTGAACCGCC CACAGTCGAC TTGGATTTAT CTCTTAACAG CAACTACTCT 
GCTTCTCCTG GAGAATCTCC CTCTGCTAAT GCAACAGCTC TGTGGGATGA TTACATTGAT 
CTACCTCGAC ACAGAGAGAA GCAGGTTAAT GAAAGTCCAA TTGTATTGCA AGACTCTCAA 
CTTCATCAAA ATGTATTGAG ACAAGCGTCC CACCCTTCCA AACCTCATGG TGATGAGTCT 
CGACCAGAGC TGCTTTTGAA TCTGAAGCTG AGCAGCCTGG CCATCTCGGT CCTCCACATC 
GACCCGTTGC CACCACCGGA TGCAGCTCAC AGTCCCCTCG GTCCTATGGC TGCACACTTC 
TTCCGCACAG TAGGGCCCCA GCATCTTGCC CCAGCGGCTT TCCTTGAGTC TCGAAACATT 
TTTAACCAGG CCTGCCCCCA TGACAATCTC AGGTTTGTGG CCCAGGGGCT CAAGATCAGC 
TATGAGCACT GTAAGGGATC CAGTCTGCGG ACCTTCAACA CCGACGTCTC TCTCAACCAG 
ATGGAGTTTT TAGAGTGCCT GTTTCCATCT GAGGGTTTCA GCAGCGACTC CCAAAGAGGC 
ATTCAGTACA CAGAGCTCCT GACATTTGAC ATGGCGAGCC CTGATGTGCC TCTGCAGACC 
TGCCTTCACC TGCTGTACAA ACAGAAAGAG AGGAAAGGCC CTCAGAGCGG TCAGTTTCGA 
CTCAGCACCA TTCCCAGGAA AGCAGAAATC CAGGTGGAGC TGGGCCCGGT GCGATGCGAG 
CTGGACATTA GTATTGTAGA CCGTCTCAAC TCGTTGCTCC AACCCCAGAA GCTGGCCGCC 
ACAGAGCTGA TGGCGTCTCA CCTGTACACA TCCTATAATA AACACGTCAG CCTGCATAAA 
GCCTTTACAG AGGTTTTTCT GGATGACAGT CACACTCCCA GCAACTGTCA CGTGTCATTG 
ACTGTAAACG CGCCGGTGCT GGGCCTCGCT CTCCGCTTCC CTATTCCTGA CCTGCGCTCA 
GATCAGGAAC GGGGCCCCTG GTTTAAGAAG TCCCTGCAGA AGGAAGTCCT CTTTCTGGAA 
CTGGAAGATC TGGAAGTCAA AACAGAATTC ATGGCTGGCA ACTCACCTGA TCAAACTAAG 
TTGGAGCTCA CATTCAGGGA GCTCATTGGA AAATTTCAAC AGGAGACTAA CCACCCAGCC 
GTCCGCTTCC TCCGAGTGTC TCACACCATG GACGGAGACA TGACAACGTC AGAAAGTGTT 
AAATTTGACT GGCCGAGGGT CGTGCTGAAG ATGAACCCCA CTGCAGTGCA CTCGATACTT 
GAGCGGGTGC AGGCCGAAGA TGATGAGGGG GTAGAGGACC ATTCACTCGA AGAGGAAGAG 
GAGGAGGGCG CTGCACATTC ACTGAAGGAC GTGTGTGACT TTGGTAAAGC AGAGCCATCT 
CCGTTTTCAT CGCGAAGGGT CATGTACGAA AATGAGGAGA TGGTCATTCC GGGTGACGTC 
ACTGAGATGA CGGAATTCCA GGAGAAGACC ATGAGCAACT CAAGGTTCAT TGTTGAGTTG 
TGTCTCCCCA ATGTGCAGTT ATCCTTCCCC AGCAAAGCAT TTTATGAAAC ACTGCACAAC 
AGGATAAATA ACGATTTGCT GCTTTGGGAG CCGACTGCTC CATCTCCCGT GGAGACGGTG 
GAAAACATCC CTTATGGAGT TGGCCTCTCT GTCGCCAGTC AGTTGATCAA CACTTACTCC 
AAAGACAGCT TTAGTCAGTT TCGCTCCTCT GGTCCTGATG ATGAGACCAG CGGGTCAGAT 
GAAGAAACCA TGCATTATTA CATTCCTGCT TCAGACCCAA GCCTCCAGTC TCGCAAGAAG 
AAAAAGCCCA AAGCCCAAAG CAAGACCTCC CAGAGCCTAT TTTCTGTCAT CGTCAGTGTC 
AATCACGGTC TGATCGCGCT ACAAGCTAAT GCTATGAAGG ACAAAACTGT ATTGAAAAAC 
AAACATGGTG AATTCTGGCT GGAGCTAAAG AATGGCGTGT TGTTCAGTGT CATGCAGCAC 
GAGGGCTACA AAGATCAACA TTACATCTGT TTCCACACCA GCAGTGTTTG CATGTATCAC 
CAAGGACTTG TGGATGGAGG CCTTTCCGTC TTGGACGTCA ATTTGCCGTC CAGGACACAT 
CCTCACTGGT TAGATCCAAC TATCTACCAA TCAGAGACTT CCCCCGAGAG ATCTTCAACG 
CCCTCAGAGT GCATCGGCCT GGAGGCTCGG AGCATGGTGT CCGTGGCCGT CAAAGTATCA 
TCACCGAACC CAGAGCACAA CATCAAGGAA ATTCTTATTG CTGTTGGAGT GAGAGGAGCC 
ACCTTGCAGC ATAAAATGGC CCCTCCTTGC ACGGGTTGGT ACGATCAGAT TGTTGACTTT 
CTGAATATCA CCAATGAGCC AGTGTTGGGA TACGCCCCTC CCACCTCGGT CTCCACCCTA 
CACTTACACC TGTGGAGCTG TTCTCTTGAC TACAGACCCC TTTATCTGCC ACTCAGATCA 
CTGTTGGTTG TAGAGACCTT TAGTATCTCC AGCAGTGTGT CTTTAGATCA CTCTTCCTCA 
TCACTCAGGA TCATATTGGA TGAGGCTGCC GTGTTCCTGT CAGACAGGGC CAATGCCGTC 
TCTGTCAATC TGGCACGTGA CTATGTGCAA GTGGTGGACA TGGGAACTCT GGAGCTCAGG 
ATCACAGCTG CCAAACCTGG AGCTGACGGA AAGCTGAAAG AGCCAAGGTT CGAACTGCGC 
TGCTCCAGCG ACATTATTCA CATCAGAACC TGTTCGGACT CCTGTGCTGC CCTCATGAAC 
CTTATTCAGT ATGTAGCCAG CTATGGAGAC TTACTGCCCC CAGCAGAACC AGAGCCCAAA 
CACAGCAGCA CCTCACAGAG GACCAAGGTT GAATTTCCAA GTCGGGTCAC ACCTCAGACG 
TCGTTTCTGG CTGATATTGA GCAGCAGATG TTGCAGGATT TGATGAGCGA AGCGATGGAG 
GAGACAGAAG GCCAGCATGC ATCAGGGCTG CAGCAGAATG GCGCACGAGA AGAACACTGT 
CAAGATCACG ATCCACCCCG CTCAGACCTG TTCTTGTTCC CGGATGAGAG CGGAAATCTT 
AACCAAGATT TAAGCCCCAC TTGTCCCGTC CTTCACTCTC CTCTCATCAC CCCTGTTCCC 
AATCTGGCCC AAGAGGCTGA TGACTTTTGT ATCCTGGAGA CACCCGGCTT CAGACGAGAG 
GATCTTGATC AGGAACCAGT GGTAAAGCGG CTTACTTCAG ATCCTGTGGT CATTAAAGAT 
GACCATTTCA GCCAGCCCTT GTGTGGGAGT GACTCAAGCC ACGGAGCCCT GAACTTTCCC 
ATCCCAGAGG TGCGCTACCT CATCAAGGAG ATCTCTGTCA TCTGGCACCT CTACGGTGGG 
AAAGACTTTG GGAGCACAAC GTTAACAGCA TCTCCTTCTA GAAGTTGGGG TTCTACACCT 
CATAGCTCCC CCTCCCAGAC TCCGGTCCGA CAATCCAAGA GTTCAAGCCG TTCTGGGGGA 
GGGAGAGGAA GGAACACTGA TGTGTTGATG GAGATACAGC TCAGCAAGGT GCGGTTTCAG 
CACGATGTGT ACCCGCAGGC TCAGGGGGCT TCTGGGCCGA CAGTGGATCA GCCGGTGTCT 
CGACAGGTGT TTGTGGTCCA GGACTTGGAG ATTCGAGACA GACTAGCTGC CTCACAGATG 
AATAAGTTCC TTTACTTGTA TTCGAGCAAG GAAATGCCCA GAAAAGCTCA TTCAAATATG 
TTGACAGTTA AGGCTCTCCA CATGTGTCCG GAGTCCAGCC AGGCTCCACA GGAATGCTGC 
TTGCGTGTTT CACTCACGCC CCTTCGCCTC AATATTGATC AGGATGCACT GTTTTTCTTG 
AAGGACTTCT TTACCAGTCT TGCTACAGAG GTGGAGTTTT TCTCATCGCC ATCACAAGAA 
GCCATCTGTG TTCCTATTAA AAAAGCAGCC ACCCCAGAGA TCTCTTGTAG CTTCTCCAAA 
CACGCCAGCA GCAGCCACGA CCCAGCCCCA ATCATCTCGC TGCCCACTCA GAGAGGTTTG 
AATCACAATG GCTTCTCCAG CAGGGAGGAA ATGGCTGACA GTGAATCTTC TGCACCCTCG 
TTCACAGACC AGCCCATATT CTTTAGGGAG TTCAGATTTA CCTCTGAGGT TCCAATTCGT 
CTGGATTACC ATGGAAAACA CGTGTCGATG GAGCAGGGGA CCTTTGCTGG AATAATTATG 
GGGTTGACAC AACTTAACTG CTCAGAGTTG AAATTGAGGC GCTTGTGCTA CAGACAAGGA 
TTATTAGGTG TGGATAAGTT GTTTTCCTAT GCAATCAACG AATGGCTTAC TGACATAAAG 
AAGAATCAGC TTCCGGGGCT GTTGGGTGGT GTGGGGCCTA TTCACTCACT GGTCCAGCTG 
GTTCAGGGAT TCAGGGATTT GGTCTGGCTC CCCATCGAGC AGTACAGGAA AGACGGTCGG 
ATTGTTCGTG GTTTTCAGAG GGGCACTGCC TCTTTCGGCA CGTCGACAGC CATGGCCGCC 
CTGGAGCTCA CCAACAGGAT GGTGCGGACC ATCCAGGCAG CAGCAGAGAC AGCCTACGAC 
ATGGTGTCTC CAGTCCCTGA TGAGAGAGAC ACAAAGAGGG TAAAACGGTA CATCCCTTAC 
GGACTGGCTC ATCAGCCCGT CGACCTGAGA GAGGGCGTGG CCAAAGCTTA CACAGTAGTA 
AAAGAGGGCA TTACAGACAC GGCACTGACC ATCTATGACA CAGCCACCAG GGAGCACGAA 
CAGCGCGGGA TGACAGGGGC AGTGGGCGGA GTTCTCCGCC AGCTGCCGCC AGCTGTGGTC 
AAGCCGCTCA TCGTGGCCAC TGAGGCCACC TCTAATGTCT TAGGTGGAAT GAGGAACCAG 
ATTCACCCTG ACGCAAGGCA GGAAGAGTCA CAGAAGTGGA GGCAGGGCGA AGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

atg2b ( XM_011617068.2 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011615370.2 Takifugu rubripes autophagy related 2B (atg2b), transcript variant X3, mRNA.

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CloneID	OTo73021
Clone ID Related Accession (Same CDS sequence)	XM_011617068.2
Accession Version	XM_011617068.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5877bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	autophagy-related protein 2 homolog B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042286.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011617068.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCTGGT CCAGTTTTAT GACAAGCAGC ATGCAACTGG CCAAAGAATG CCTAAGCCAG 
AAGCTCACGG ATGACCTCGG AGAGAGTTTT CAGCCTTTTG AAGGATTAGA GAAGTTTGCC 
GAAACAATAG AAACAGTCCT GAGGAGAGTT AAAGTGACAT TTTTGGATAC AGTTCTCAGA 
ATTGAACACA TTCCAGAAAA TTCTAAAACT GGAATCGCGC TGGAGATTCG AATTAACAAG 
ACGGTTTACT GTGATGAAAC TAACGAGGAG ACTTCAGACG TTAACGTGCA TCAGCCGACC 
ACGTTTGCCC ACAAAAACCT GCAGATGGAA GGCATCACTG TGTTCTGGGA CGAGTTCTCC 
GAGGTGTCCC GCGCTGGCTG TAAATCTTCA CCAACTCCCA CGGAAACCGA ACCAAAACTG 
TCCCCGAGCT GGAATCCTAA AATAATCTGC GAGCCGCATC CCCAGTTCAC AGAACCAGTA 
TACTCTGCAG CACCATTTGA AGCTGTGCAG GTCGGGAGCC TTGGTGGGAA AATCAAGATC 
TCCCTCACTC TGAAGAACAA CCTAGCTATG CCCGGAGCAA AGCTGGATGT TGACGGTCAC 
ATCGACACAC TGTTGATTCT GCTGTCGCCG CGACAAGTTC ATCTTCTTTT GGACCTATTC 
GGAGCCTTTT CCGGTGGAGG TTCACAAGAA TGGAGCAAGG ACAGAAAGAG CAGACCCATG 
CAACAGGAGG ACGAGTACCG GCTCCACATG GAGCTGAACC GGTGTCTAAA GAAAGACACC 
ACGGTCACAG GAGTCGACCC TGAATTGTTT GAAAGCCAAA CCACAAGAAC CGTGTCGAGT 
CGAGACGACG TGTTCTTTTC CATGGCCGAC ATGGATATGT CTCACAGCCT GTCGTCGCTG 
CCTCCTCTTG GTGAACCGCC CACAGTCGAC TTGGATTTAT CTCTTAACAG CAACTACTCT 
GCTTCTCCTG GAGAATCTCC CTCTGCTAAT GCAACAGCTC TGTGGGATGA TTACATTGAT 
CTACCTCGAC ACAGAGAGAA GCAGGTTAAT GAAAGTCCAA TTGTATTGCA AGACTCTCAA 
CTTCATCAAA ATGTATTGAG ACAAGCGTCC CACCCTTCCA AACCTCATGG TGATGAGTCT 
CGACCAGAGC TGCTTTTGAA TCTGAAGCTG AGCAGCCTGG CCATCTCGGT CCTCCACATC 
GACCCGTTGC CACCACCGGA TGCAGCTCAC AGTCCCCTCG GTCCTATGGC TGCACACTTC 
TTCCGCACAG TAGGGCCCCA GCATCTTGCC CCAGCGGCTT TCCTTGAGTC TCGAAACATT 
TTTAACCAGG CCTGCCCCCA TGACAATCTC AGGTTTGTGG CCCAGGGGCT CAAGATCAGC 
TATGAGCACT GTAAGGGATC CAGTCTGCGG ACCTTCAACA CCGACGTCTC TCTCAACCAG 
ATGGAGTTTT TAGAGTGCCT GTTTCCATCT GAGGGTTTCA GCAGCGACTC CCAAAGAGGC 
ATTCAGTACA CAGAGCTCCT GACATTTGAC ATGGCGAGCC CTGATGTGCC TCTGCAGACC 
TGCCTTCACC TGCTGTACAA ACAGAAAGAG AGGAAAGGCC CTCAGAGCGG TCAGTTTCGA 
CTCAGCACCA TTCCCAGGAA AGCAGAAATC CAGGTGGAGC TGGGCCCGGT GCGATGCGAG 
CTGGACATTA GTATTGTAGA CCGTCTCAAC TCGTTGCTCC AACCCCAGAA GCTGGCCGCC 
ACAGAGCTGA TGGCGTCTCA CCTGTACACA TCCTATAATA AACACGTCAG CCTGCATAAA 
GCCTTTACAG AGGTTTTTCT GGATGACAGT CACACTCCCA GCAACTGTCA CGTGTCATTG 
ACTGTAAACG CGCCGGTGCT GGGCCTCGCT CTCCGCTTCC CTATTCCTGA CCTGCGCTCA 
GATCAGGAAC GGGGCCCCTG GTTTAAGAAG TCCCTGCAGA AGGAAGTCCT CTTTCTGGAA 
CTGGAAGATC TGGAAGTCAA AACAGAATTC ATGGCTGGCA ACTCACCTGA TCAAACTAAG 
TTGGAGCTCA CATTCAGGGA GCTCATTGGA AAATTTCAAC AGGAGACTAA CCACCCAGCC 
GTCCGCTTCC TCCGAGTGTC TCACACCATG GACGGAGACA TGACAACGTC AGAAAGTGTT 
AAATTTGACT GGCCGAGGGT CGTGCTGAAG ATGAACCCCA CTGCAGTGCA CTCGATACTT 
GAGCGGGTGC AGGCCGAAGA TGATGAGGGG GTAGAGGACC ATTCACTCGA AGAGGAAGAG 
GAGGAGGGCG CTGCACATTC ACTGAAGGAC GTGTGTGACT TTGGTAAAGC AGAGCCATCT 
CCGTTTTCAT CGCGAAGGGT CATGTACGAA AATGAGGAGA TGGTCATTCC GGGTGACGTC 
ACTGAGATGA CGGAATTCCA GGAGAAGACC ATGAGCAACT CAAGGTTCAT TGTTGAGTTG 
TGTCTCCCCA ATGTGCAGTT ATCCTTCCCC AGCAAAGCAT TTTATGAAAC ACTGCACAAC 
AGGATAAATA ACGATTTGCT GCTTTGGGAG CCGACTGCTC CATCTCCCGT GGAGACGGTG 
GAAAACATCC CTTATGGAGT TGGCCTCTCT GTCGCCAGTC AGTTGATCAA CACTTACTCC 
AAAGACAGCT TTAGTCAGTT TCGCTCCTCT GGTCCTGATG ATGAGACCAG CGGGTCAGAT 
GAAGAAACCA TGCATTATTA CATTCCTGCT TCAGACCCAA GCCTCCAGTC TCGCAAGAAG 
AAAAAGCCCA AAGCCCAAAG CAAGACCTCC CAGAGCCTAT TTTCTGTCAT CGTCAGTGTC 
AATCACGGTC TGATCGCGCT ACAAGCTAAT GCTATGAAGG ACAAAACTGT ATTGAAAAAC 
AAACATGGTG AATTCTGGCT GGAGCTAAAG AATGGCGTGT TGTTCAGTGT CATGCAGCAC 
GAGGGCTACA AAGATCAACA TTACATCTGT TTCCACACCA GCAGTGTTTG CATGTATCAC 
CAAGGACTTG TGGATGGAGG CCTTTCCGTC TTGGACGTCA ATTTGCCGTC CAGGACACAT 
CCTCACTGGT TAGATCCAAC TATCTACCAA TCAGAGACTT CCCCCGAGAG ATCTTCAACG 
CCCTCAGAGT GCATCGGCCT GGAGGCTCGG AGCATGGTGT CCGTGGCCGT CAAAGTATCA 
TCACCGAACC CAGAGCACAA CATCAAGGAA ATTCTTATTG CTGTTGGAGT GAGAGGAGCC 
ACCTTGCAGC ATAAAATGGC CCCTCCTTGC ACGGGTTGGT ACGATCAGAT TGTTGACTTT 
CTGAATATCA CCAATGAGCC AGTGTTGGGA TACGCCCCTC CCACCTCGGT CTCCACCCTA 
CACTTACACC TGTGGAGCTG TTCTCTTGAC TACAGACCCC TTTATCTGCC ACTCAGATCA 
CTGTTGGTTG TAGAGACCTT TAGTATCTCC AGCAGTGTGT CTTTAGATCA CTCTTCCTCA 
TCACTCAGGA TCATATTGGA TGAGGCTGCC GTGTTCCTGT CAGACAGGGC CAATGCCGTC 
TCTGTCAATC TGGCACGTGA CTATGTGCAA GTGGTGGACA TGGGAACTCT GGAGCTCAGG 
ATCACAGCTG CCAAACCTGG AGCTGACGGA AAGCTGAAAG AGCCAAGGTT CGAACTGCGC 
TGCTCCAGCG ACATTATTCA CATCAGAACC TGTTCGGACT CCTGTGCTGC CCTCATGAAC 
CTTATTCAGT ATGTAGCCAG CTATGGAGAC TTACTGCCCC CAGCAGAACC AGAGCCCAAA 
CACAGCAGTA CCTCACAGAG GACCAAGGTT GAATTTCCAA GTCGGGTCAC ACCTCAGACG 
TCGTTTCTGG CTGATATTGA GCAGCAGATG TTGCAGGATT TGATGAGCGA AGCGATGGAG 
GAGACAGAAG GCCAGCATGC ATCAGGGCTG CAGCAGAATG GCGCACGAGA AGAACACTGT 
CAAGATCACG ATCCACCCCG CTCAGACCTG TTCTTGTTCC CGGATGAGAG CGGAAATCTT 
AACCAAGATT TAAGCCCCAC TTGTCCCGTC CTTCACTCTC CTCTCATCAC CCCTGTTCCC 
AATCTGGCCC AAGAGGCTGA TGACTTTTGT ATCCTGGAGA CACCCGGCTT CAGACGAGAG 
GATCTTGATC AGGAACCAGT GGTAAAGCGG CTTACTTCAG ATCCTGTGGT CATTAAAGAT 
GACCATTTCA GCCAGCCCTT GTGTGGGAGT GACTCAAGCC ACGGAGCCCT GAACTTTCCC 
ATCCCAGAGG TGCGCTACCT CATCAAGGAG ATCTCTGTCA TCTGGCACCT CTACGGTGGG 
AAAGACTTTG GGAGCACAAC GTTAACAGCA TCTCCTTCTA GAAGTTGGGG TTCTACACCT 
CATAGCTCCC CCTCCCAGAC TCCGGTCCGA CAATCCAAGA GTTCAAGCCG TTCTGGGGGA 
GGGAGAGGAA GGAACACTGA TGTGTTGATG GAGATACAGC TCAGCAAGGT GCGGTTTCAG 
CACGATGTGT ACCCGCAGGC TCAGGGGGCT TCTGGGCCGA CAGTGGATCA GCCGGTGTCT 
CGACAGGTGT TTGTGGTCCA GGACTTGGAG ATTCGAGACA GACTAGCTGC CTCACAGATG 
AATAAGTTCC TTTACTTGTA TTCGAGCAAG GAAATGCCCA GAAAAGCTCA TTCAAATATG 
TTGACAGTTA AGGCTCTCCA CATGTGTCCG GAGTCCAGCC AGGCTCCACA GGAATGCTGC 
TTGCGTGTTT CACTCACGCC CCTTCGCCTC AATATTGATC AGGATGCACT GTTTTTCTTG 
AAGGACTTCT TTACCAGTCT TGCTACAGAG GTGGAGTTTT TCTCATCGCC ATCACAAGAA 
GCCATCTGTG TTCCTATTAA AAAAGCAGCC ACCCCAGAGA TCTCTTGTAG CTTCTCCAAA 
CACGCCAGCA GCAGCCACGA CCCAGCCCCA ATCATCTCGC TGCCCACTCA GAGAGGTTTG 
AATCACAATG GCTTCTCCAG CAGGGAGGAA ATGGCTGACA GTGAATCTTC TGCACCCTCG 
TTCACAGACC AGCCCATATT CTTTAGGGAG TTCAGATTTA CCTCTGAGGT TCCAATTCGT 
CTGGATTACC ATGGAAAACA CGTGTCGATG GAGCAGGGGA CCTTTGCTGG AATAATTATG 
GGGTTGACAC AACTTAACTG CTCAGAGTTG AAATTGAGGC GCTTGTGCTA CAGACAAGGA 
TTATTAGGTG TGGATAAGTT GTTTTCCTAT GCAATCAACG AATGGCTTAC TGACATAAAG 
AAGAATCAGC TTCCGGGGCT GTTGGGTGGT GTGGGGCCTA TTCACTCACT GGTCCAGCTG 
GTTCAGGGAT TCAGGGATTT GGTCTGGCTC CCCATCGAGC AGTACAGGAA AGACGGTCGG 
ATTGTTCGTG GTTTTCAGAG GGGCACTGCC TCTTTCGGCA CGTCGACAGC CATGGCCGCC 
CTGGAGCTCA CCAACAGGAT GGTGCGGACC ATCCAGGCAG CAGCAGAGAC AGCCTACGAC 
ATGGTGTCTC CAGTCCCTGA TGAGAGAGAC ACAAAGAGGG TAAAACGGTA CATCCCTTAC 
AGACTGGCTC ATCAGCCCGT CGACCTGAGA GAGGGCGTGG CCAAAGCTTA CACAGTAGTA 
AAAGAGGGCA TTACAGACAC GGCACTGACC ATCTATGACA CAGCCACCAG GGAGCACGAA 
CAGCGCGGGA TGACAGGGGC AGTGGGCGGA GTTCTCCGCC AGCTGCCGCC AGCTGTGGTC 
AAGCCGCTCA TCGTGGCCAC TGAGGCCACC TCTAATGTCT TAGGTGGAAT GAGGAACCAG 
ATTCACCCTG ACGCAAGGCA GGAAGAGTCA CAGAAGTGGA GGCAGGGCGA AGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011615370.2
CDS	152..6028
Translation	MCWSSFMTSSMQLAKECLSQKLTDDLGESFQPFEGLEKFAETIETVLRRVKVTFLDTVLRIEHIPENSKTGIALEIRINKTVYCDETNEETSDVNVHQPTTFAHKNLQMEGITVFWDEFSEVSRAGCKSSPTPTETEPKLSPSWNPKIICEPHPQFTEPVYSAAPFEAVQVGSLGGKIKISLTLKNNLAMPGAKLDVDGHIDTLLILLSPRQVHLLLDLFGAFSGGGSQEWSKDRKSRPMQQEDEYRLHMELNRCLKKDTTVTGVDPELFESQTTRTVSSRDDVFFSMADMDMSHSLSSLPPLGEPPTVDLDLSLNSNYSASPGESPSANATALWDDYIDLPRHREKQVNESPIVLQDSQLHQNVLRQASHPSKPHGDESRPELLLNLKLSSLAISVLHIDPLPPPDAAHSPLGPMAAHFFRTVGPQHLAPAAFLESRNIFNQACPHDNLRFVAQGLKISYEHCKGSSLRTFNTDVSLNQMEFLECLFPSEGFSSDSQRGIQYTELLTFDMASPDVPLQTCLHLLYKQKERKGPQSGQFRLSTIPRKAEIQVELGPVRCELDISIVDRLNSLLQPQKLAATELMASHLYTSYNKHVSLHKAFTEVFLDDSHTPSNCHVSLTVNAPVLGLALRFPIPDLRSDQERGPWFKKSLQKEVLFLELEDLEVKTEFMAGNSPDQTKLELTFRELIGKFQQETNHPAVRFLRVSHTMDGDMTTSESVKFDWPRVVLKMNPTAVHSILERVQAEDDEGVEDHSLEEEEEEGAAHSLKDVCDFGKAEPSPFSSRRVMYENEEMVIPGDVTEMTEFQEKTMSNSRFIVELCLPNVQLSFPSKAFYETLHNRINNDLLLWEPTAPSPVETVENIPYGVGLSVASQLINTYSKDSFSQFRSSGPDDETSGSDEETMHYYIPASDPSLQSRKKKKPKAQSKTSQSLFSVIVSVNHGLIALQANAMKDKTVLKNKHGEFWLELKNGVLFSVMQHEGYKDQHYICFHTSSVCMYHQGLVDGGLSVLDVNLPSRTHPHWLDPTIYQSETSPERSSTPSECIGLEARSMVSVAVKVSSPNPEHNIKEILIAVGVRGATLQHKMAPPCTGWYDQIVDFLNITNEPVLGYAPPTSVSTLHLHLWSCSLDYRPLYLPLRSLLVVETFSISSSVSLDHSSSSLRIILDEAAVFLSDRANAVSVNLARDYVQVVDMGTLELRITAAKPGADGKLKEPRFELRCSSDIIHIRTCSDSCAALMNLIQYVASYGDLLPPAEPEPKHSSTSQRTKVEFPSRVTPQTSFLADIEQQMLQDLMSEAMEETEGQHASGLQQNGAREEHCQDHDPPRSDLFLFPDESGNLNQDLSPTCPVLHSPLITPVPNLAQEADDFCILETPGFRREDLDQEPVVKRLTSDPVVIKDDHFSQPLCGSDSSHGALNFPIPEVRYLIKEISVIWHLYGGKDFGSTTLTASPSRSWGSTPHSSPSQTPVRQSKSSSRSGGGRGRNTDVLMEIQLSKVRFQHDVYPQAQGASGPTVDQPVSRQVFVVQDLEIRDRLAASQMNKFLYLYSSKEMPRKAHSNMLTVKALHMCPESSQAPQECCLRVSLTPLRLNIDQDALFFLKDFFTSLATEVEFFSSPSQEAICVPIKKAATPEISCSFSKHASSSHDPAPIISLPTQRGLNHNGFSSREEMADSESSAPSFTDQPIFFREFRFTSEVPIRLDYHGKHVSMEQGTFAGIIMGLTQLNCSELKLRRLCYRQGLLGVDKLFSYAINEWLTDIKKNQLPGLLGGVGPIHSLVQLVQGFRDLVWLPIEQYRKDGRIVRGFQRGTASFGTSTAMAALELTNRMVRTIQAAAETAYDMVSPVPDERDTKRVKRYIPYRLAHQPVDLREGVAKAYTVVKEGITDTALTIYDTATREHEQRGMTGAVGGVLRQLPPAVVKPLIVATEATSNVLGGMRNQIHPDARQEESQKWRQGEE

Target ORF information:

RefSeq Version	XM_011617068.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes autophagy related 2B (atg2b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011617068.2

ORF Insert Sequence:

ATGTGCTGGT CCAGTTTTAT GACAAGCAGC ATGCAACTGG CCAAAGAATG CCTAAGCCAG 
AAGCTCACGG ATGACCTCGG AGAGAGTTTT CAGCCTTTTG AAGGATTAGA GAAGTTTGCC 
GAAACAATAG AAACAGTCCT GAGGAGAGTT AAAGTGACAT TTTTGGATAC AGTTCTCAGA 
ATTGAACACA TTCCAGAAAA TTCTAAAACT GGAATCGCGC TGGAGATTCG AATTAACAAG 
ACGGTTTACT GTGATGAAAC TAACGAGGAG ACTTCAGACG TTAACGTGCA TCAGCCGACC 
ACGTTTGCCC ACAAAAACCT GCAGATGGAA GGCATCACTG TGTTCTGGGA CGAGTTCTCC 
GAGGTGTCCC GCGCTGGCTG TAAATCTTCA CCAACTCCCA CGGAAACCGA ACCAAAACTG 
TCCCCGAGCT GGAATCCTAA AATAATCTGC GAGCCGCATC CCCAGTTCAC AGAACCAGTA 
TACTCTGCAG CACCATTTGA AGCTGTGCAG GTCGGGAGCC TTGGTGGGAA AATCAAGATC 
TCCCTCACTC TGAAGAACAA CCTAGCTATG CCCGGAGCAA AGCTGGATGT TGACGGTCAC 
ATCGACACAC TGTTGATTCT GCTGTCGCCG CGACAAGTTC ATCTTCTTTT GGACCTATTC 
GGAGCCTTTT CCGGTGGAGG TTCACAAGAA TGGAGCAAGG ACAGAAAGAG CAGACCCATG 
CAACAGGAGG ACGAGTACCG GCTCCACATG GAGCTGAACC GGTGTCTAAA GAAAGACACC 
ACGGTCACAG GAGTCGACCC TGAATTGTTT GAAAGCCAAA CCACAAGAAC CGTGTCGAGT 
CGAGACGACG TGTTCTTTTC CATGGCCGAC ATGGATATGT CTCACAGCCT GTCGTCGCTG 
CCTCCTCTTG GTGAACCGCC CACAGTCGAC TTGGATTTAT CTCTTAACAG CAACTACTCT 
GCTTCTCCTG GAGAATCTCC CTCTGCTAAT GCAACAGCTC TGTGGGATGA TTACATTGAT 
CTACCTCGAC ACAGAGAGAA GCAGGTTAAT GAAAGTCCAA TTGTATTGCA AGACTCTCAA 
CTTCATCAAA ATGTATTGAG ACAAGCGTCC CACCCTTCCA AACCTCATGG TGATGAGTCT 
CGACCAGAGC TGCTTTTGAA TCTGAAGCTG AGCAGCCTGG CCATCTCGGT CCTCCACATC 
GACCCGTTGC CACCACCGGA TGCAGCTCAC AGTCCCCTCG GTCCTATGGC TGCACACTTC 
TTCCGCACAG TAGGGCCCCA GCATCTTGCC CCAGCGGCTT TCCTTGAGTC TCGAAACATT 
TTTAACCAGG CCTGCCCCCA TGACAATCTC AGGTTTGTGG CCCAGGGGCT CAAGATCAGC 
TATGAGCACT GTAAGGGATC CAGTCTGCGG ACCTTCAACA CCGACGTCTC TCTCAACCAG 
ATGGAGTTTT TAGAGTGCCT GTTTCCATCT GAGGGTTTCA GCAGCGACTC CCAAAGAGGC 
ATTCAGTACA CAGAGCTCCT GACATTTGAC ATGGCGAGCC CTGATGTGCC TCTGCAGACC 
TGCCTTCACC TGCTGTACAA ACAGAAAGAG AGGAAAGGCC CTCAGAGCGG TCAGTTTCGA 
CTCAGCACCA TTCCCAGGAA AGCAGAAATC CAGGTGGAGC TGGGCCCGGT GCGATGCGAG 
CTGGACATTA GTATTGTAGA CCGTCTCAAC TCGTTGCTCC AACCCCAGAA GCTGGCCGCC 
ACAGAGCTGA TGGCGTCTCA CCTGTACACA TCCTATAATA AACACGTCAG CCTGCATAAA 
GCCTTTACAG AGGTTTTTCT GGATGACAGT CACACTCCCA GCAACTGTCA CGTGTCATTG 
ACTGTAAACG CGCCGGTGCT GGGCCTCGCT CTCCGCTTCC CTATTCCTGA CCTGCGCTCA 
GATCAGGAAC GGGGCCCCTG GTTTAAGAAG TCCCTGCAGA AGGAAGTCCT CTTTCTGGAA 
CTGGAAGATC TGGAAGTCAA AACAGAATTC ATGGCTGGCA ACTCACCTGA TCAAACTAAG 
TTGGAGCTCA CATTCAGGGA GCTCATTGGA AAATTTCAAC AGGAGACTAA CCACCCAGCC 
GTCCGCTTCC TCCGAGTGTC TCACACCATG GACGGAGACA TGACAACGTC AGAAAGTGTT 
AAATTTGACT GGCCGAGGGT CGTGCTGAAG ATGAACCCCA CTGCAGTGCA CTCGATACTT 
GAGCGGGTGC AGGCCGAAGA TGATGAGGGG GTAGAGGACC ATTCACTCGA AGAGGAAGAG 
GAGGAGGGCG CTGCACATTC ACTGAAGGAC GTGTGTGACT TTGGTAAAGC AGAGCCATCT 
CCGTTTTCAT CGCGAAGGGT CATGTACGAA AATGAGGAGA TGGTCATTCC GGGTGACGTC 
ACTGAGATGA CGGAATTCCA GGAGAAGACC ATGAGCAACT CAAGGTTCAT TGTTGAGTTG 
TGTCTCCCCA ATGTGCAGTT ATCCTTCCCC AGCAAAGCAT TTTATGAAAC ACTGCACAAC 
AGGATAAATA ACGATTTGCT GCTTTGGGAG CCGACTGCTC CATCTCCCGT GGAGACGGTG 
GAAAACATCC CTTATGGAGT TGGCCTCTCT GTCGCCAGTC AGTTGATCAA CACTTACTCC 
AAAGACAGCT TTAGTCAGTT TCGCTCCTCT GGTCCTGATG ATGAGACCAG CGGGTCAGAT 
GAAGAAACCA TGCATTATTA CATTCCTGCT TCAGACCCAA GCCTCCAGTC TCGCAAGAAG 
AAAAAGCCCA AAGCCCAAAG CAAGACCTCC CAGAGCCTAT TTTCTGTCAT CGTCAGTGTC 
AATCACGGTC TGATCGCGCT ACAAGCTAAT GCTATGAAGG ACAAAACTGT ATTGAAAAAC 
AAACATGGTG AATTCTGGCT GGAGCTAAAG AATGGCGTGT TGTTCAGTGT CATGCAGCAC 
GAGGGCTACA AAGATCAACA TTACATCTGT TTCCACACCA GCAGTGTTTG CATGTATCAC 
CAAGGACTTG TGGATGGAGG CCTTTCCGTC TTGGACGTCA ATTTGCCGTC CAGGACACAT 
CCTCACTGGT TAGATCCAAC TATCTACCAA TCAGAGACTT CCCCCGAGAG ATCTTCAACG 
CCCTCAGAGT GCATCGGCCT GGAGGCTCGG AGCATGGTGT CCGTGGCCGT CAAAGTATCA 
TCACCGAACC CAGAGCACAA CATCAAGGAA ATTCTTATTG CTGTTGGAGT GAGAGGAGCC 
ACCTTGCAGC ATAAAATGGC CCCTCCTTGC ACGGGTTGGT ACGATCAGAT TGTTGACTTT 
CTGAATATCA CCAATGAGCC AGTGTTGGGA TACGCCCCTC CCACCTCGGT CTCCACCCTA 
CACTTACACC TGTGGAGCTG TTCTCTTGAC TACAGACCCC TTTATCTGCC ACTCAGATCA 
CTGTTGGTTG TAGAGACCTT TAGTATCTCC AGCAGTGTGT CTTTAGATCA CTCTTCCTCA 
TCACTCAGGA TCATATTGGA TGAGGCTGCC GTGTTCCTGT CAGACAGGGC CAATGCCGTC 
TCTGTCAATC TGGCACGTGA CTATGTGCAA GTGGTGGACA TGGGAACTCT GGAGCTCAGG 
ATCACAGCTG CCAAACCTGG AGCTGACGGA AAGCTGAAAG AGCCAAGGTT CGAACTGCGC 
TGCTCCAGCG ACATTATTCA CATCAGAACC TGTTCGGACT CCTGTGCTGC CCTCATGAAC 
CTTATTCAGT ATGTAGCCAG CTATGGAGAC TTACTGCCCC CAGCAGAACC AGAGCCCAAA 
CACAGCAGTA CCTCACAGAG GACCAAGGTT GAATTTCCAA GTCGGGTCAC ACCTCAGACG 
TCGTTTCTGG CTGATATTGA GCAGCAGATG TTGCAGGATT TGATGAGCGA AGCGATGGAG 
GAGACAGAAG GCCAGCATGC ATCAGGGCTG CAGCAGAATG GCGCACGAGA AGAACACTGT 
CAAGATCACG ATCCACCCCG CTCAGACCTG TTCTTGTTCC CGGATGAGAG CGGAAATCTT 
AACCAAGATT TAAGCCCCAC TTGTCCCGTC CTTCACTCTC CTCTCATCAC CCCTGTTCCC 
AATCTGGCCC AAGAGGCTGA TGACTTTTGT ATCCTGGAGA CACCCGGCTT CAGACGAGAG 
GATCTTGATC AGGAACCAGT GGTAAAGCGG CTTACTTCAG ATCCTGTGGT CATTAAAGAT 
GACCATTTCA GCCAGCCCTT GTGTGGGAGT GACTCAAGCC ACGGAGCCCT GAACTTTCCC 
ATCCCAGAGG TGCGCTACCT CATCAAGGAG ATCTCTGTCA TCTGGCACCT CTACGGTGGG 
AAAGACTTTG GGAGCACAAC GTTAACAGCA TCTCCTTCTA GAAGTTGGGG TTCTACACCT 
CATAGCTCCC CCTCCCAGAC TCCGGTCCGA CAATCCAAGA GTTCAAGCCG TTCTGGGGGA 
GGGAGAGGAA GGAACACTGA TGTGTTGATG GAGATACAGC TCAGCAAGGT GCGGTTTCAG 
CACGATGTGT ACCCGCAGGC TCAGGGGGCT TCTGGGCCGA CAGTGGATCA GCCGGTGTCT 
CGACAGGTGT TTGTGGTCCA GGACTTGGAG ATTCGAGACA GACTAGCTGC CTCACAGATG 
AATAAGTTCC TTTACTTGTA TTCGAGCAAG GAAATGCCCA GAAAAGCTCA TTCAAATATG 
TTGACAGTTA AGGCTCTCCA CATGTGTCCG GAGTCCAGCC AGGCTCCACA GGAATGCTGC 
TTGCGTGTTT CACTCACGCC CCTTCGCCTC AATATTGATC AGGATGCACT GTTTTTCTTG 
AAGGACTTCT TTACCAGTCT TGCTACAGAG GTGGAGTTTT TCTCATCGCC ATCACAAGAA 
GCCATCTGTG TTCCTATTAA AAAAGCAGCC ACCCCAGAGA TCTCTTGTAG CTTCTCCAAA 
CACGCCAGCA GCAGCCACGA CCCAGCCCCA ATCATCTCGC TGCCCACTCA GAGAGGTTTG 
AATCACAATG GCTTCTCCAG CAGGGAGGAA ATGGCTGACA GTGAATCTTC TGCACCCTCG 
TTCACAGACC AGCCCATATT CTTTAGGGAG TTCAGATTTA CCTCTGAGGT TCCAATTCGT 
CTGGATTACC ATGGAAAACA CGTGTCGATG GAGCAGGGGA CCTTTGCTGG AATAATTATG 
GGGTTGACAC AACTTAACTG CTCAGAGTTG AAATTGAGGC GCTTGTGCTA CAGACAAGGA 
TTATTAGGTG TGGATAAGTT GTTTTCCTAT GCAATCAACG AATGGCTTAC TGACATAAAG 
AAGAATCAGC TTCCGGGGCT GTTGGGTGGT GTGGGGCCTA TTCACTCACT GGTCCAGCTG 
GTTCAGGGAT TCAGGGATTT GGTCTGGCTC CCCATCGAGC AGTACAGGAA AGACGGTCGG 
ATTGTTCGTG GTTTTCAGAG GGGCACTGCC TCTTTCGGCA CGTCGACAGC CATGGCCGCC 
CTGGAGCTCA CCAACAGGAT GGTGCGGACC ATCCAGGCAG CAGCAGAGAC AGCCTACGAC 
ATGGTGTCTC CAGTCCCTGA TGAGAGAGAC ACAAAGAGGG TAAAACGGTA CATCCCTTAC 
AGACTGGCTC ATCAGCCCGT CGACCTGAGA GAGGGCGTGG CCAAAGCTTA CACAGTAGTA 
AAAGAGGGCA TTACAGACAC GGCACTGACC ATCTATGACA CAGCCACCAG GGAGCACGAA 
CAGCGCGGGA TGACAGGGGC AGTGGGCGGA GTTCTCCGCC AGCTGCCGCC AGCTGTGGTC 
AAGCCGCTCA TCGTGGCCAC TGAGGCCACC TCTAATGTCT TAGGTGGAAT GAGGAACCAG 
ATTCACCCTG ACGCAAGGCA GGAAGAGTCA CAGAAGTGGA GGCAGGGCGA AGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

atg2b ( XM_011617041.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011615343.1 Takifugu rubripes autophagy related 2B (atg2b), transcript variant X1, mRNA.

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CloneID	OTo24747
Clone ID Related Accession (Same CDS sequence)	XM_011617041.1
Accession Version	XM_011617041.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426348800000
Organism	Takifugu rubripes(Fugu rubripes)
Product	autophagy-related protein 2 homolog B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070921.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTGGC CTTTTTCTGA GTCCATCAAG AAGCGGGCCT GCAGATATCT CCTGCATAGG 
TACCTTGGCA ACTTTTTGGA AGAGAAACTA AGCTTGGATC AGCTCAGTTT AGATCTCTAT 
CAAGGTACTG GCTCACTTTC GCAGGTGCCG CTTGATAAAT GGTCGCTGAA TGAACTCCTG 
GAGACAGCAG ATGCACCGTT TGAAGTCATT ACTGGGTTTA TCCAGACCAT TTCTTTAACT 
GTCCCATGGG CAGCGCTTGT GCAGGAAAAT TGTGCCCTTG AGGTCAAAGG GTTAGAGATG 
GTCTTCAGAC CAAAACCAAG AGTAGCATCT GGCACTGAGC CCATGTGCTG GTCCAGTTTT 
ATGACAAGCA GCATGCAACT GGCCAAAGAA TGCCTAAGCC AGAAGCTCAC GGATGACCTC 
GGAGAGAGTT TTCAGCCTTT TGAAGGATTA GAGAAGTTTG CCGAAACAAT AGAAACAGTC 
CTGAGGAGAG TTAAAGTGAC ATTTTTGGAT ACAGTTCTCA GAATTGAACA CATTCCAGAA 
AATTCTAAAA CTGGAATCGC GCTGGAGATT CGAATTAACA AGACGGTTTA CTGTGATGAA 
ACTAACGAGG AGACTTCAGA CGTTAACGTG CATCAGCCGA CCACGTTTGC CCACAAAAAC 
CTGCAGATGG AAGGCATCAC TGTGTTCTGG GACGAGTTCT CCGAGGTGTC CCGCGCTGGC 
TGTAAATCTT CACCAACTCC CACGGAAACC GAACCAAAAC TGTCCCCGAG CTGGAATCCT 
AAAATAATCT GCGAGCCGCA TCCCCAGTTC ACAGAACCAG TATACTCTGC AGCACCATTT 
GAAGCTGTGC AGGTCGGGAG CCTTGGTGGG AAAATCAAGA TCTCCCTCAC TCTGAAGAAC 
AACCTAGCTA TGCCCGGAGC AAAGCTGGAT GTTGACGGTC ACATCGACAC ACTGTTGATT 
CTGCTGTCGC CGCGACAAGT TCATCTTCTT TTGGACCTAT TCGGAGCCTT TTCCGGTGGA 
GGTTCACAAG AATGGAGCAA GGACAGAAAG AGCAGACCCA TGCAACAGGA GGACGAGTAC 
CGGCTCCACA TGGAGCTGAA CCGGTGTCTA AAGAAAGACA CCACGGTCAC AGGAGTCGAC 
CCTGAATTGT TTGAAAGCCA AACCACAAGA ACCGTGTCGA GTCGAGACGA CGTGTTCTTT 
TCCATGGCCG ACATGGATAT GTCTCACAGC CTGTCGTCGC TGCCTCCTCT TGGTGAACCG 
CCCACAGTCG ACTTGGATTT ATCTCTTAAC AGCAACTACT CTGCTTCTCC TGGAGAATCT 
CCCTCTGCTA ATGCAACAGC TCTGTGGGAT GATTACATTG ATCTACCTCG ACACAGAGAG 
AAGCAGGTTA ATGAAAGTCC AATTGTATTG CAAGACTCTC AACTTCATCA AAATGTATTG 
AGACAAGCGT CCCACCCTTC CAAACCTCAT GGTGATGAGT CTCGACCAGA GCTGCTTTTG 
AATCTGAAGC TGAGCAGCCT GGCCATCTCG GTCCTCCACA TCGACCCGTT GCCACCACCG 
GATGCAGCTC ACAGTCCCCT CGGTCCTATG GCTGCACACT TCTTCCGCAC AGTAGGGCCC 
CAGCATCTTG CCCCAGCGGC TTTCCTTGAG TCTCGAAACA TTTTTAACCA GGCCTGCCCC 
CATGACAATC TCAGGTTTGT GGCCCAGGGG CTCAAGATCA GCTATGAGCA CTGTAAGGGA 
TCCAGTCTGC GGACCTTCAA CACCGACGTC TCTCTCAACC AGATGGAGTT TTTAGAGTGC 
CTGTTTCCAT CTGAGGGTTT CAGCAGCGAC TCCCAAAGAG GCATTCAGTA CACAGAGCTC 
CTGACATTTG ACATGGCGAG CCCTGATGTG CCTCTGCAGA CCTGCCTTCA CCTGCTGTAC 
AAACAGAAAG AGAGGAAAGG CCCTCAGAGC GGTCAGTTTC GACTCAGCAC CATTCCCAGG 
AAAGCAGAAA TCCAGGTGGA GCTGGGCCCG GTGCGATGCG AGCTGGACAT TAGTATTGTA 
GACCGTCTCA ACTCGTTGCT CCAACCCCAG AAGCTGGCCG CCACAGAGCT GATGGCGTCT 
CACCTGTACA CATCCTATAA TAAACACGTC AGCCTGCATA AAGCCTTTAC AGAGGTTTTT 
CTGGATGACA GTCACACTCC CAGCAACTGT CACGTGTCAT TGACTGTAAA CGCGCCGGTG 
CTGGGCCTCG CTCTCCGCTT CCCTATTCCT GACCTGCGCT CAGATCAGGA ACGGGGCCCC 
TGGTTTAAGA AGTCCCTGCA GAAGGAAGTC CTCTTTCTGG AACTGGAAGA TCTGGAAGTC 
AAAACAGAAT TCATGGCTGG CAACTCACCT GATCAAACTA AGTTGGAGCT CACATTCAGG 
GAGCTCATTG GAAAATTTCA ACAGGAGACT AACCACCCAG CCGTCCGCTT CCTCCGAGTG 
TCTCACACCA TGGACGGAGA CATGACAACG TCAGAAAGTG TTAAATTTGA CTGGCCGAGG 
GTCGTGCTGA AGATGAACCC CACTGCAGTG CACTCGATAC TTGAGCGGGT GCAGGCCGAA 
GATGATGAGG GGGTAGAGGA CCATTCACTC GAAGAGGAAG AGGAGGAGGG CGCTGCACAT 
TCACTGAAGG ACGTGTGTGA CTTTGGTAAA GCAGAGCCAT CTCCGTTTTC ATCGCGAAGG 
GTCATGTACG AAAATGAGGA GATGGTCATT CCGGGTGACG TCACTGAGAT GACGGAATTC 
CAGGAGAAGA CCATGAGCAA CTCAAGGTTC ATTGTTGAGT TGTGTCTCCC CAATGTGCAG 
TTATCCTTCC CCAGCAAAGC ATTTTATGAA ACACTGCACA ACAGGATAAA TAACGATTTG 
CTGCTTTGGG AGCCGACTGC TCCATCTCCC GTGGAGACGG TGGAAAACAT CCCTTATGGA 
GTTGGCCTCT CTGTCGCCAG TCAGTTGATC AACACTTACT CCAAAGACAG CTTTAGTCAG 
TTTCGCTCCT CTGGTCCTGA TGATGAGACC AGCGGGTCAG ATGAAGAAAC CATGCATTAT 
TACATTCCTG CTTCAGACCC AAGCCTCCAG TCTCGCAAGA AGAAAAAGCC CAAAGCCCAA 
AGCAAGACCT CCCAGAGCCT ATTTTCTGTC ATCGTCAGTG TCAATCACGG TCTGATCGCG 
CTACAAGCTA ATGCTATGAA GGACAAAACT GTATTGAAAA ACAAACATGG TGAATTCTGG 
CTGGAGCTAA AGAATGGCGT GTTGTTCAGT GTCATGCAGC ACGAGGGCTA CAAAGATCAA 
CATTACATCT GTTTCCACAC CAGCAGTGTT TGCATGTATC ACCAAGGACT TGTGGATGGA 
GGCCTTTCCG TCTTGGACGT CAATTTGCCG TCCAGGACAC ATCCTCACTG GTTAGATCCA 
ACTATCTACC AATCAGAGAC TTCCCCCGAG AGATCTTCAA CGCCCTCAGA GTGCATCGGC 
CTGGAGGCTC GGAGCATGGT GTCCGTGGCC GTCAAAGTAT CATCACCGAA CCCAGAGCAC 
AACATCAAGG AAATTCTTAT TGCTGTTGGA GTGAGAGGAG CCACCTTGCA GCATAAAATG 
GCCCCTCCTT GCACGGGTTG GTACGATCAG ATTGTTGACT TTCTGAATAT CACCAATGAG 
CCAGTGTTGG GATACGCCCC TCCCACCTCG GTCTCCACCC TACACTTACA CCTGTGGAGC 
TGTTCTCTTG ACTACAGACC CCTTTATCTG CCACTCAGAT CACTGTTGGT TGTAGAGACC 
TTTAGTATCT CCAGCAGTGT GTCTTTAGAT CACTCTTCCT CATCACTCAG GATCATATTG 
GATGAGGCTG CCGTGTTCCT GTCAGACAGG GCCAATGCCG TCTCTGTCAA TCTGGCACGT 
GACTATGTGC AAGTGGTGGA CATGGGAACT CTGGAGCTCA GGATCACAGC TGCCAAACCT 
GGAGCTGACG GAAAGCTGAA AGAGCCAAGG TTCGAACTGC GCTGCTCCAG CGACATTATT 
CACATCAGAA CCTGTTCGGA CTCCTGTGCT GCCCTCATGA ACCTTATTCA GTATGTAGCC 
AGCTATGGAG ACTTACTGCC CCCAGCAGAA CCAGAGCCCA AACACAGCAG CACCTCACAG 
AGGACCAAGG TTGAATTTCC AAGTCGGGTC ACACCTCAGA CGTCGTTTCT GGCTGATATT 
GAGCAGCAGA TGTTGCAGGA TTTGATGAGC GAAGCGATGG AGGAGACAGA AGGCCAGCAT 
GCATCAGGGC TGCAGCAGAA TGGCGCACGA GAAGAACACT GTCAAGATCA CGATCCACCC 
CGCTCAGACC TGTTCTTGTT CCCGGATGAG AGCGGAAATC TTAACCAAGA TTTAAGCCCC 
ACTTGTCCCG TCCTTCACTC TCCTCTCATC ACCCCTGTTC CCAATCTGGC CCAAGAGGCT 
GATGACTTTT GTATCCTGGA GACACCCGGC TTCAGACGAG AGGATCTTGA TCAGGAACCA 
GTGGTAAAGC GGCTTACTTC AGATCCTGTG GTCATTAAAG ATGACCATTT CAGCCAGCCC 
TTGTGTGGGA GTGACTCAAG CCACGGAGCC CTGAACTTTC CCATCCCAGA GGTGCGCTAC 
CTCATCAAGG AGATCTCTGT CATCTGGCAC CTCTACGGTG GGAAAGACTT TGGGAGCACA 
ACGTTAACAG CATCTCCTTC TAGAAGTTGG GGTTCTACAC CTCATAGCTC CCCCTCCCAG 
ACTCCGGTCC GACAATCCAA GAGTTCAAGC CGTTCTGGGG GAGGGAGAGG AAGGAACACT 
GATGTGTTGA TGGAGATACA GCTCAGCAAG GTGCGGTTTC AGCACGATGT GTACCCGCAG 
GCTCAGGGGG CTTCTGGGCC GACAGTGGAT CAGCCGGTGT CTCGACAGGT GTTTGTGGTC 
CAGGACTTGG AGATTCGAGA CAGACTAGCT GCCTCACAGA TGAATAAGTT CCTTTACTTG 
TATTCGAGCA AGGAAATGCC CAGAAAAGCT CATTCAAATA TGTTGACAGT TAAGGCTCTC 
CACATGTGTC CGGAGTCCAG CCAGGCTCCA CAGGAATGCT GCTTGCGTGT TTCACTCACG 
CCCCTTCGCC TCAATATTGA TCAGGATGCA CTGTTTTTCT TGAAGGACTT CTTTACCAGT 
CTTGCTACAG AGGTGGAGTT TTTCTCATCG CCATCACAAG AAGCCATCTG TGTTCCTATT 
AAAAAAGCAG CCACCCCAGA GATCTCTTGT AGCTTCTCCA AACACGCCAG CAGCAGCCAC 
GACCCAGCCC CAATCATCTC GCTGCCCACT CAGAGAGGTT TGAATCACAA TGGCTTCTCC 
AGCAGGGAGG AAATGGCTGA CAGTGAATCT TCTGCACCCT CGTTCACAGA CCAGCCCATA 
TTCTTTAGGG AGTTCAGATT TACCTCTGAG GTTCCAATTC GTCTGGATTA CCATGGAAAA 
CACGTGTCGA TGGAGCAGGG GACCTTTGCT GGAATAATTA TGGGGTTGAC ACAACTTAAC 
TGCTCAGAGT TGAAATTGAG GCGCTTGTGC TACAGACAAG GATTATTAGG TGTGGATAAG 
TTGTTTTCCT ATGCAATCAA CGAATGGCTT ACTGACATAA AGAAGAATCA GCTTCCGGGG 
CTGTTGGGTG GTGTGGGGCC TATTCACTCA CTGGTCCAGC TGGTTCAGGG ATTCAGGGAT 
TTGGTCTGGC TCCCCATCGA GCAGTACAGG AAAGACGGTC GGATTGTTCG TGGTTTTCAG 
AGGGGCACTG CCTCTTTCGG CACGTCGACA GCCATGGCCG CCCTGGAGCT CACCAACAGG 
ATGGTGCGGA CCATCCAGGC AGCAGCAGAG ACAGCCTACG ACATGGTGTC TCCAGTCCCT 
GATGAGAGAG ACACAAAGAG GGTAAAACGG TACATCCCTT ACGGACTGGC TCATCAGCCC 
GTCGACCTGA GAGAGGGCGT GGCCAAAGCT TACACAGTAG TAAAAGAGGG CATTACAGAC 
ACGGCACTGA CCATCTATGA CACAGCCACC AGGGAGCACG AACAGCGCGG GATGACAGGG 
GCAGTGGGCG GAGTTCTCCG CCAGCTGCCG CCAGCTGTGG TCAAGCCGCT CATCGTGGCC 
ACTGAGGCCA CCTCTAATGT CTTAGGTGGA ATGAGGAACC AGATTCACCC TGACGCAAGG 
CAGGAAGAGT CACAGAAGTG GAGGCAGGGC GAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011615343.1
CDS	320..6538
Translation	MPWPFSESIKKRACRYLLHRYLGNFLEEKLSLDQLSLDLYQGTGSLSQVPLDKWSLNELLETADAPFEVITGFIQTISLTVPWAALVQENCALEVKGLEMVFRPKPRVASGTEPMCWSSFMTSSMQLAKECLSQKLTDDLGESFQPFEGLEKFAETIETVLRRVKVTFLDTVLRIEHIPENSKTGIALEIRINKTVYCDETNEETSDVNVHQPTTFAHKNLQMEGITVFWDEFSEVSRAGCKSSPTPTETEPKLSPSWNPKIICEPHPQFTEPVYSAAPFEAVQVGSLGGKIKISLTLKNNLAMPGAKLDVDGHIDTLLILLSPRQVHLLLDLFGAFSGGGSQEWSKDRKSRPMQQEDEYRLHMELNRCLKKDTTVTGVDPELFESQTTRTVSSRDDVFFSMADMDMSHSLSSLPPLGEPPTVDLDLSLNSNYSASPGESPSANATALWDDYIDLPRHREKQVNESPIVLQDSQLHQNVLRQASHPSKPHGDESRPELLLNLKLSSLAISVLHIDPLPPPDAAHSPLGPMAAHFFRTVGPQHLAPAAFLESRNIFNQACPHDNLRFVAQGLKISYEHCKGSSLRTFNTDVSLNQMEFLECLFPSEGFSSDSQRGIQYTELLTFDMASPDVPLQTCLHLLYKQKERKGPQSGQFRLSTIPRKAEIQVELGPVRCELDISIVDRLNSLLQPQKLAATELMASHLYTSYNKHVSLHKAFTEVFLDDSHTPSNCHVSLTVNAPVLGLALRFPIPDLRSDQERGPWFKKSLQKEVLFLELEDLEVKTEFMAGNSPDQTKLELTFRELIGKFQQETNHPAVRFLRVSHTMDGDMTTSESVKFDWPRVVLKMNPTAVHSILERVQAEDDEGVEDHSLEEEEEEGAAHSLKDVCDFGKAEPSPFSSRRVMYENEEMVIPGDVTEMTEFQEKTMSNSRFIVELCLPNVQLSFPSKAFYETLHNRINNDLLLWEPTAPSPVETVENIPYGVGLSVASQLINTYSKDSFSQFRSSGPDDETSGSDEETMHYYIPASDPSLQSRKKKKPKAQSKTSQSLFSVIVSVNHGLIALQANAMKDKTVLKNKHGEFWLELKNGVLFSVMQHEGYKDQHYICFHTSSVCMYHQGLVDGGLSVLDVNLPSRTHPHWLDPTIYQSETSPERSSTPSECIGLEARSMVSVAVKVSSPNPEHNIKEILIAVGVRGATLQHKMAPPCTGWYDQIVDFLNITNEPVLGYAPPTSVSTLHLHLWSCSLDYRPLYLPLRSLLVVETFSISSSVSLDHSSSSLRIILDEAAVFLSDRANAVSVNLARDYVQVVDMGTLELRITAAKPGADGKLKEPRFELRCSSDIIHIRTCSDSCAALMNLIQYVASYGDLLPPAEPEPKHSSTSQRTKVEFPSRVTPQTSFLADIEQQMLQDLMSEAMEETEGQHASGLQQNGAREEHCQDHDPPRSDLFLFPDESGNLNQDLSPTCPVLHSPLITPVPNLAQEADDFCILETPGFRREDLDQEPVVKRLTSDPVVIKDDHFSQPLCGSDSSHGALNFPIPEVRYLIKEISVIWHLYGGKDFGSTTLTASPSRSWGSTPHSSPSQTPVRQSKSSSRSGGGRGRNTDVLMEIQLSKVRFQHDVYPQAQGASGPTVDQPVSRQVFVVQDLEIRDRLAASQMNKFLYLYSSKEMPRKAHSNMLTVKALHMCPESSQAPQECCLRVSLTPLRLNIDQDALFFLKDFFTSLATEVEFFSSPSQEAICVPIKKAATPEISCSFSKHASSSHDPAPIISLPTQRGLNHNGFSSREEMADSESSAPSFTDQPIFFREFRFTSEVPIRLDYHGKHVSMEQGTFAGIIMGLTQLNCSELKLRRLCYRQGLLGVDKLFSYAINEWLTDIKKNQLPGLLGGVGPIHSLVQLVQGFRDLVWLPIEQYRKDGRIVRGFQRGTASFGTSTAMAALELTNRMVRTIQAAAETAYDMVSPVPDERDTKRVKRYIPYGLAHQPVDLREGVAKAYTVVKEGITDTALTIYDTATREHEQRGMTGAVGGVLRQLPPAVVKPLIVATEATSNVLGGMRNQIHPDARQEESQKWRQGEE

Target ORF information:

RefSeq Version	XM_011617041.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes autophagy related 2B (atg2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011617041.1

ORF Insert Sequence:

ATGCCTTGGC CTTTTTCTGA GTCCATCAAG AAGCGGGCCT GCAGATATCT CCTGCATAGG 
TACCTTGGCA ACTTTTTGGA AGAGAAACTA AGCTTGGATC AGCTCAGTTT AGATCTCTAT 
CAAGGTACTG GCTCACTTTC GCAGGTGCCG CTTGATAAAT GGTCGCTGAA TGAACTCCTG 
GAGACAGCAG ATGCACCGTT TGAAGTCATT ACTGGGTTTA TCCAGACCAT TTCTTTAACT 
GTCCCATGGG CAGCGCTTGT GCAGGAAAAT TGTGCCCTTG AGGTCAAAGG GTTAGAGATG 
GTCTTCAGAC CAAAACCAAG AGTAGCATCT GGCACTGAGC CCATGTGCTG GTCCAGTTTT 
ATGACAAGCA GCATGCAACT GGCCAAAGAA TGCCTAAGCC AGAAGCTCAC GGATGACCTC 
GGAGAGAGTT TTCAGCCTTT TGAAGGATTA GAGAAGTTTG CCGAAACAAT AGAAACAGTC 
CTGAGGAGAG TTAAAGTGAC ATTTTTGGAT ACAGTTCTCA GAATTGAACA CATTCCAGAA 
AATTCTAAAA CTGGAATCGC GCTGGAGATT CGAATTAACA AGACGGTTTA CTGTGATGAA 
ACTAACGAGG AGACTTCAGA CGTTAACGTG CATCAGCCGA CCACGTTTGC CCACAAAAAC 
CTGCAGATGG AAGGCATCAC TGTGTTCTGG GACGAGTTCT CCGAGGTGTC CCGCGCTGGC 
TGTAAATCTT CACCAACTCC CACGGAAACC GAACCAAAAC TGTCCCCGAG CTGGAATCCT 
AAAATAATCT GCGAGCCGCA TCCCCAGTTC ACAGAACCAG TATACTCTGC AGCACCATTT 
GAAGCTGTGC AGGTCGGGAG CCTTGGTGGG AAAATCAAGA TCTCCCTCAC TCTGAAGAAC 
AACCTAGCTA TGCCCGGAGC AAAGCTGGAT GTTGACGGTC ACATCGACAC ACTGTTGATT 
CTGCTGTCGC CGCGACAAGT TCATCTTCTT TTGGACCTAT TCGGAGCCTT TTCCGGTGGA 
GGTTCACAAG AATGGAGCAA GGACAGAAAG AGCAGACCCA TGCAACAGGA GGACGAGTAC 
CGGCTCCACA TGGAGCTGAA CCGGTGTCTA AAGAAAGACA CCACGGTCAC AGGAGTCGAC 
CCTGAATTGT TTGAAAGCCA AACCACAAGA ACCGTGTCGA GTCGAGACGA CGTGTTCTTT 
TCCATGGCCG ACATGGATAT GTCTCACAGC CTGTCGTCGC TGCCTCCTCT TGGTGAACCG 
CCCACAGTCG ACTTGGATTT ATCTCTTAAC AGCAACTACT CTGCTTCTCC TGGAGAATCT 
CCCTCTGCTA ATGCAACAGC TCTGTGGGAT GATTACATTG ATCTACCTCG ACACAGAGAG 
AAGCAGGTTA ATGAAAGTCC AATTGTATTG CAAGACTCTC AACTTCATCA AAATGTATTG 
AGACAAGCGT CCCACCCTTC CAAACCTCAT GGTGATGAGT CTCGACCAGA GCTGCTTTTG 
AATCTGAAGC TGAGCAGCCT GGCCATCTCG GTCCTCCACA TCGACCCGTT GCCACCACCG 
GATGCAGCTC ACAGTCCCCT CGGTCCTATG GCTGCACACT TCTTCCGCAC AGTAGGGCCC 
CAGCATCTTG CCCCAGCGGC TTTCCTTGAG TCTCGAAACA TTTTTAACCA GGCCTGCCCC 
CATGACAATC TCAGGTTTGT GGCCCAGGGG CTCAAGATCA GCTATGAGCA CTGTAAGGGA 
TCCAGTCTGC GGACCTTCAA CACCGACGTC TCTCTCAACC AGATGGAGTT TTTAGAGTGC 
CTGTTTCCAT CTGAGGGTTT CAGCAGCGAC TCCCAAAGAG GCATTCAGTA CACAGAGCTC 
CTGACATTTG ACATGGCGAG CCCTGATGTG CCTCTGCAGA CCTGCCTTCA CCTGCTGTAC 
AAACAGAAAG AGAGGAAAGG CCCTCAGAGC GGTCAGTTTC GACTCAGCAC CATTCCCAGG 
AAAGCAGAAA TCCAGGTGGA GCTGGGCCCG GTGCGATGCG AGCTGGACAT TAGTATTGTA 
GACCGTCTCA ACTCGTTGCT CCAACCCCAG AAGCTGGCCG CCACAGAGCT GATGGCGTCT 
CACCTGTACA CATCCTATAA TAAACACGTC AGCCTGCATA AAGCCTTTAC AGAGGTTTTT 
CTGGATGACA GTCACACTCC CAGCAACTGT CACGTGTCAT TGACTGTAAA CGCGCCGGTG 
CTGGGCCTCG CTCTCCGCTT CCCTATTCCT GACCTGCGCT CAGATCAGGA ACGGGGCCCC 
TGGTTTAAGA AGTCCCTGCA GAAGGAAGTC CTCTTTCTGG AACTGGAAGA TCTGGAAGTC 
AAAACAGAAT TCATGGCTGG CAACTCACCT GATCAAACTA AGTTGGAGCT CACATTCAGG 
GAGCTCATTG GAAAATTTCA ACAGGAGACT AACCACCCAG CCGTCCGCTT CCTCCGAGTG 
TCTCACACCA TGGACGGAGA CATGACAACG TCAGAAAGTG TTAAATTTGA CTGGCCGAGG 
GTCGTGCTGA AGATGAACCC CACTGCAGTG CACTCGATAC TTGAGCGGGT GCAGGCCGAA 
GATGATGAGG GGGTAGAGGA CCATTCACTC GAAGAGGAAG AGGAGGAGGG CGCTGCACAT 
TCACTGAAGG ACGTGTGTGA CTTTGGTAAA GCAGAGCCAT CTCCGTTTTC ATCGCGAAGG 
GTCATGTACG AAAATGAGGA GATGGTCATT CCGGGTGACG TCACTGAGAT GACGGAATTC 
CAGGAGAAGA CCATGAGCAA CTCAAGGTTC ATTGTTGAGT TGTGTCTCCC CAATGTGCAG 
TTATCCTTCC CCAGCAAAGC ATTTTATGAA ACACTGCACA ACAGGATAAA TAACGATTTG 
CTGCTTTGGG AGCCGACTGC TCCATCTCCC GTGGAGACGG TGGAAAACAT CCCTTATGGA 
GTTGGCCTCT CTGTCGCCAG TCAGTTGATC AACACTTACT CCAAAGACAG CTTTAGTCAG 
TTTCGCTCCT CTGGTCCTGA TGATGAGACC AGCGGGTCAG ATGAAGAAAC CATGCATTAT 
TACATTCCTG CTTCAGACCC AAGCCTCCAG TCTCGCAAGA AGAAAAAGCC CAAAGCCCAA 
AGCAAGACCT CCCAGAGCCT ATTTTCTGTC ATCGTCAGTG TCAATCACGG TCTGATCGCG 
CTACAAGCTA ATGCTATGAA GGACAAAACT GTATTGAAAA ACAAACATGG TGAATTCTGG 
CTGGAGCTAA AGAATGGCGT GTTGTTCAGT GTCATGCAGC ACGAGGGCTA CAAAGATCAA 
CATTACATCT GTTTCCACAC CAGCAGTGTT TGCATGTATC ACCAAGGACT TGTGGATGGA 
GGCCTTTCCG TCTTGGACGT CAATTTGCCG TCCAGGACAC ATCCTCACTG GTTAGATCCA 
ACTATCTACC AATCAGAGAC TTCCCCCGAG AGATCTTCAA CGCCCTCAGA GTGCATCGGC 
CTGGAGGCTC GGAGCATGGT GTCCGTGGCC GTCAAAGTAT CATCACCGAA CCCAGAGCAC 
AACATCAAGG AAATTCTTAT TGCTGTTGGA GTGAGAGGAG CCACCTTGCA GCATAAAATG 
GCCCCTCCTT GCACGGGTTG GTACGATCAG ATTGTTGACT TTCTGAATAT CACCAATGAG 
CCAGTGTTGG GATACGCCCC TCCCACCTCG GTCTCCACCC TACACTTACA CCTGTGGAGC 
TGTTCTCTTG ACTACAGACC CCTTTATCTG CCACTCAGAT CACTGTTGGT TGTAGAGACC 
TTTAGTATCT CCAGCAGTGT GTCTTTAGAT CACTCTTCCT CATCACTCAG GATCATATTG 
GATGAGGCTG CCGTGTTCCT GTCAGACAGG GCCAATGCCG TCTCTGTCAA TCTGGCACGT 
GACTATGTGC AAGTGGTGGA CATGGGAACT CTGGAGCTCA GGATCACAGC TGCCAAACCT 
GGAGCTGACG GAAAGCTGAA AGAGCCAAGG TTCGAACTGC GCTGCTCCAG CGACATTATT 
CACATCAGAA CCTGTTCGGA CTCCTGTGCT GCCCTCATGA ACCTTATTCA GTATGTAGCC 
AGCTATGGAG ACTTACTGCC CCCAGCAGAA CCAGAGCCCA AACACAGCAG CACCTCACAG 
AGGACCAAGG TTGAATTTCC AAGTCGGGTC ACACCTCAGA CGTCGTTTCT GGCTGATATT 
GAGCAGCAGA TGTTGCAGGA TTTGATGAGC GAAGCGATGG AGGAGACAGA AGGCCAGCAT 
GCATCAGGGC TGCAGCAGAA TGGCGCACGA GAAGAACACT GTCAAGATCA CGATCCACCC 
CGCTCAGACC TGTTCTTGTT CCCGGATGAG AGCGGAAATC TTAACCAAGA TTTAAGCCCC 
ACTTGTCCCG TCCTTCACTC TCCTCTCATC ACCCCTGTTC CCAATCTGGC CCAAGAGGCT 
GATGACTTTT GTATCCTGGA GACACCCGGC TTCAGACGAG AGGATCTTGA TCAGGAACCA 
GTGGTAAAGC GGCTTACTTC AGATCCTGTG GTCATTAAAG ATGACCATTT CAGCCAGCCC 
TTGTGTGGGA GTGACTCAAG CCACGGAGCC CTGAACTTTC CCATCCCAGA GGTGCGCTAC 
CTCATCAAGG AGATCTCTGT CATCTGGCAC CTCTACGGTG GGAAAGACTT TGGGAGCACA 
ACGTTAACAG CATCTCCTTC TAGAAGTTGG GGTTCTACAC CTCATAGCTC CCCCTCCCAG 
ACTCCGGTCC GACAATCCAA GAGTTCAAGC CGTTCTGGGG GAGGGAGAGG AAGGAACACT 
GATGTGTTGA TGGAGATACA GCTCAGCAAG GTGCGGTTTC AGCACGATGT GTACCCGCAG 
GCTCAGGGGG CTTCTGGGCC GACAGTGGAT CAGCCGGTGT CTCGACAGGT GTTTGTGGTC 
CAGGACTTGG AGATTCGAGA CAGACTAGCT GCCTCACAGA TGAATAAGTT CCTTTACTTG 
TATTCGAGCA AGGAAATGCC CAGAAAAGCT CATTCAAATA TGTTGACAGT TAAGGCTCTC 
CACATGTGTC CGGAGTCCAG CCAGGCTCCA CAGGAATGCT GCTTGCGTGT TTCACTCACG 
CCCCTTCGCC TCAATATTGA TCAGGATGCA CTGTTTTTCT TGAAGGACTT CTTTACCAGT 
CTTGCTACAG AGGTGGAGTT TTTCTCATCG CCATCACAAG AAGCCATCTG TGTTCCTATT 
AAAAAAGCAG CCACCCCAGA GATCTCTTGT AGCTTCTCCA AACACGCCAG CAGCAGCCAC 
GACCCAGCCC CAATCATCTC GCTGCCCACT CAGAGAGGTT TGAATCACAA TGGCTTCTCC 
AGCAGGGAGG AAATGGCTGA CAGTGAATCT TCTGCACCCT CGTTCACAGA CCAGCCCATA 
TTCTTTAGGG AGTTCAGATT TACCTCTGAG GTTCCAATTC GTCTGGATTA CCATGGAAAA 
CACGTGTCGA TGGAGCAGGG GACCTTTGCT GGAATAATTA TGGGGTTGAC ACAACTTAAC 
TGCTCAGAGT TGAAATTGAG GCGCTTGTGC TACAGACAAG GATTATTAGG TGTGGATAAG 
TTGTTTTCCT ATGCAATCAA CGAATGGCTT ACTGACATAA AGAAGAATCA GCTTCCGGGG 
CTGTTGGGTG GTGTGGGGCC TATTCACTCA CTGGTCCAGC TGGTTCAGGG ATTCAGGGAT 
TTGGTCTGGC TCCCCATCGA GCAGTACAGG AAAGACGGTC GGATTGTTCG TGGTTTTCAG 
AGGGGCACTG CCTCTTTCGG CACGTCGACA GCCATGGCCG CCCTGGAGCT CACCAACAGG 
ATGGTGCGGA CCATCCAGGC AGCAGCAGAG ACAGCCTACG ACATGGTGTC TCCAGTCCCT 
GATGAGAGAG ACACAAAGAG GGTAAAACGG TACATCCCTT ACGGACTGGC TCATCAGCCC 
GTCGACCTGA GAGAGGGCGT GGCCAAAGCT TACACAGTAG TAAAAGAGGG CATTACAGAC 
ACGGCACTGA CCATCTATGA CACAGCCACC AGGGAGCACG AACAGCGCGG GATGACAGGG 
GCAGTGGGCG GAGTTCTCCG CCAGCTGCCG CCAGCTGTGG TCAAGCCGCT CATCGTGGCC 
ACTGAGGCCA CCTCTAATGT CTTAGGTGGA ATGAGGAACC AGATTCACCC TGACGCAAGG 
CAGGAAGAGT CACAGAAGTG GAGGCAGGGC GAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

atg2b ( XM_011617055.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011615357.1 Takifugu rubripes autophagy related 2B (atg2b), transcript variant X3, mRNA.

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CloneID	OTo24748
Clone ID Related Accession (Same CDS sequence)	XM_011617055.1 , XM_011617049.1
Accession Version	XM_011617055.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6198bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426348800000
Organism	Takifugu rubripes(Fugu rubripes)
Product	autophagy-related protein 2 homolog B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070921.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTGGC CTTTTTCTGA GTCCATCAAG AAGCGGGCCT GCAGATATCT CCTGCATAGG 
TACCTTGGCA ACTTTTTGGA AGAGAAACTA AGCTTGGATC AGCTCAGTTT AGATCTCTAT 
CAAGGTACTG GCTCACTTTC GCAGGTGCCG CTTGATAAAT GGTCGCTGAA TGAACTCCTG 
GAGACAGCAG ATGCACCGTT TGAAGTCATT ACTGGGTTTA TCCAGACCAT TTCTTTAACT 
GTCCCATGGG CAGCGCTTGT GCAGGAAAAT TGTGCCCTTG AGGTCAAAGG GTTAGAGATG 
GTCTTCAGAC CAAAACCAAG AGTAGCATCT GGCACTGAGC CCATGTGCTG GTCCAGTTTT 
ATGACAAGCA GCATGCAACT GGCCAAAGAA TGCCTAAGCC AGAAGCTCAC GGATGACCTC 
GGAGAGAGTT TTCAGCCTTT TGAAGGATTA GAGAAGTTTG CCGAAACAAT AGAAACAGTC 
CTGAGGAGAG TTAAAGTGAC ATTTTTGGAT ACAGTTCTCA GAATTGAACA CATTCCAGAA 
AATTCTAAAA CTGGAATCGC GCTGGAGATT CGAATTAACA AGACGGTTTA CTGTGATGAA 
ACTAACGAGG AGACTTCAGA CGTTAACGTG CATCAGCCGA CCACGTTTGC CCACAAAAAC 
CTGCAGATGG AAGGCATCAC TGTGTTCTGG GACGAGTTCT CCGAGGTGTC CCGCGCTGGC 
TGTAAATCTT CACCAACTCC CACGGAAACC GAACCAAAAC TGTCCCCGAG CTGGAATCCT 
AAAATAATCT GCGAGCCGCA TCCCCAGTTC ACAGAACCAG TATACTCTGC AGCACCATTT 
GAAGCTGTGC AGGTCGGGAG CCTTGGTGGG AAAATCAAGA TCTCCCTCAC TCTGAAGAAC 
AACCTAGCTA TGCCCGGAGC AAAGCTGGAT GTTGACGGTC ACATCGACAC ACTGTTGATT 
CTGCTGTCGC CGCGACAAGT TCATCTTCTT TTGGACCTAT TCGGAGCCTT TTCCGGTGGA 
GGTTCACAAG AATGGAGCAA GGACAGAAAG AGCAGACCCA TGCAACAGGA GGACGAGTAC 
CGGCTCCACA TGGAGCTGAA CCGGTGTCTA AAGAAAGACA CCACGGTCAC AGGAGTCGAC 
CCTGAATTGT TTGAAAGCCA AACCACAAGA ACCGTGTCGA GTCGAGACGA CGTGTTCTTT 
TCCATGGCCG ACATGGATAT GTCTCACAGC CTGTCGTCGC TGCCTCCTCT TGGTGAACCG 
CCCACAGTCG ACTTGGATTT ATCTCTTAAC AGCAACTACT CTGCTTCTCC TGGAGAATCT 
CCCTCTGCTA ATGCAACAGC TCTGTGGGAT GATTACATTG ATCTACCTCG ACACAGAGAG 
AAGCAGGTTA ATGAAAGTCC AATTGTATTG CAAGACTCTC AACTTCATCA AAATGTATTG 
AGACAAGCGT CCCACCCTTC CAAACCTCAT GGTGATGAGT CTCGACCAGA GCTGCTTTTG 
AATCTGAAGC TGAGCAGCCT GGCCATCTCG GTCCTCCACA TCGACCCGTT GCCACCACCG 
GATGCAGCTC ACAGTCCCCT CGGTCCTATG GCTGCACACT TCTTCCGCAC AGTAGGGCCC 
CAGCATCTTG CCCCAGCGGC TTTCCTTGAG TCTCGAAACA TTTTTAACCA GGCCTGCCCC 
CATGACAATC TCAGGTTTGT GGCCCAGGGG CTCAAGATCA GCTATGAGCA CTGTAAGGGA 
TCCAGTCTGC GGACCTTCAA CACCGACGTC TCTCTCAACC AGATGGAGTT TTTAGAGTGC 
CTGTTTCCAT CTGAGGGTTT CAGCAGCGAC TCCCAAAGAG GCATTCAGTA CACAGAGCTC 
CTGACATTTG ACATGGCGAG CCCTGATGTG CCTCTGCAGA CCTGCCTTCA CCTGCTGTAC 
AAACAGAAAG AGAGGAAAGG CCCTCAGAGC GGTCAGTTTC GACTCAGCAC CATTCCCAGG 
AAAGCAGAAA TCCAGGTGGA GCTGGGCCCG GTGCGATGCG AGCTGGACAT TAGTATTGTA 
GACCGTCTCA ACTCGTTGCT CCAACCCCAG AAGCTGGCCG CCACAGAGCT GATGGCGTCT 
CACCTGTACA CATCCTATAA TAAACACGTC AGCCTGCATA AAGCCTTTAC AGAGGTTTTT 
CTGGATGACA GTCACACTCC CAGCAACTGT CACGTGTCAT TGACTGTAAA CGCGCCGGTG 
CTGGGCCTCG CTCTCCGCTT CCCTATTCCT GACCTGCGCT CAGATCAGGA ACGGGGCCCC 
TGGTTTAAGA AGTCCCTGCA GAAGGAAGTC CTCTTTCTGG AACTGGAAGA TCTGGAAGTC 
AAAACAGAAT TCATGGCTGG CAACTCACCT GATCAAACTA AGTTGGAGCT CACATTCAGG 
GAGCTCATTG GAAAATTTCA ACAGGAGACT AACCACCCAG CCGTCCGCTT CCTCCGAGTG 
TCTCACACCA TGGACGGAGA CATGACAACG TCAGAAAGTG TTAAATTTGA CTGGCCGAGG 
GTCGTGCTGA AGATGAACCC CACTGCAGTG CACTCGATAC TTGAGCGGGT GCAGGCCGAA 
GATGATGAGG GGGTAGAGGA CCATTCACTC GAAGAGGAAG AGGAGGAGGG CGCTGCACAT 
TCACTGAAGG ACGTGTGTGA CTTTGGTAAA GCAGAGCCAT CTCCGTTTTC ATCGCGAAGG 
GTCATGTACG AAAATGAGGA GATGGTCATT CCGGGTGACG TCACTGAGAT GACGGAATTC 
CAGGAGAAGA CCATGAGCAA CTCAAGGTTC ATTGTTGAGT TGTGTCTCCC CAATGTGCAG 
TTATCCTTCC CCAGCAAAGC ATTTTATGAA ACACTGCACA ACAGGATAAA TAACGATTTG 
CTGCTTTGGG AGCCGACTGC TCCATCTCCC GTGGAGACGG TGGAAAACAT CCCTTATGGA 
GTTGGCCTCT CTGTCGCCAG TCAGTTGATC AACACTTACT CCAAAGACAG CTTTAGTCAG 
TTTCGCTCCT CTGGTCCTGA TGATGAGACC AGCGGGTCAG ATGAAGAAAC CATGCATTAT 
TACATTCCTG CTTCAGACCC AAGCCTCCAG TCTCGCAAGA AGAAAAAGCC CAAAGCCCAA 
AGCAAGACCT CCCAGAGCCT ATTTTCTGTC ATCGTCAGTG TCAATCACGG TCTGATCGCG 
CTACAAGCTA ATGCTATGAA GGACAAAACT GTATTGAAAA ACAAACATGG TGAATTCTGG 
CTGGAGCTAA AGAATGGCGT GTTGTTCAGT GTCATGCAGC ACGAGGGCTA CAAAGATCAA 
CATTACATCT GTTTCCACAC CAGCAGTGTT TGCATGTATC ACCAAGGACT TGTGGATGGA 
GGCCTTTCCG TCTTGGACGT CAATTTGCCG TCCAGGACAC ATCCTCACTG GTTAGATCCA 
ACTATCTACC AATCAGAGAC TTCCCCCGAG AGATCTTCAA CGCCCTCAGA GTGCATCGGC 
CTGGAGGCTC GGAGCATGGT GTCCGTGGCC GTCAAAGTAT CATCACCGAA CCCAGAGCAC 
AACATCAAGG AAATTCTTAT TGCTGTTGGA GTGAGAGGAG CCACCTTGCA GCATAAAATG 
GCCCCTCCTT GCACGGGTTG GTACGATCAG ATTGTTGACT TTCTGAATAT CACCAATGAG 
CCAGTGTTGG GATACGCCCC TCCCACCTCG GTCTCCACCC TACACTTACA CCTGTGGAGC 
TGTTCTCTTG ACTACAGACC CCTTTATCTG CCACTCAGAT CACTGTTGGT TGTAGAGACC 
TTTAGTATCT CCAGCAGTGT GTCTTTAGAT CACTCTTCCT CATCACTCAG GATCATATTG 
GATGAGGCTG CCGTGTTCCT GTCAGACAGG GCCAATGCCG TCTCTGTCAA TCTGGCACGT 
GACTATGTGC AAGTGGTGGA CATGGGAACT CTGGAGCTCA GGATCACAGC TGCCAAACCT 
GGAGCTGACG GAAAGCTGAA AGAGCCAAGG TTCGAACTGC GCTGCTCCAG CGACATTATT 
CACATCAGAA CCTGTTCGGA CTCCTGTGCT GCCCTCATGA ACCTTATTCA GTATGTAGCC 
AGCTATGGAG ACTTACTGCC CCCAGCAGAA CCAGAGCCCA AACACAGCAG CACCTCACAG 
AGGACCAAGG TTGAATTTCC AAGTCGGGTC ACACCTCAGA CGTCGTTTCT GGCTGATATT 
GAGCAGCAGA TGTTGCAGGA TTTGATGAGC GAAGCGATGG AGGAGACAGA AGGCCAGCAT 
GCATCAGGGC TGCAGCAGAA TGGCGCACGA GAAGAACACT GTCAAGATCA CGATCCACCC 
CGCTCAGACC TGTTCTTGTT CCCGGATGAG AGCGGAAATC TTAACCAAGA TTTAAGCCCC 
ACTTGTCCCG TCCTTCACTC TCCTCTCATC ACCCCTGTTC CCAATCTGGC CCAAGAGGCT 
GATGACTTTT GTATCCTGGA GACACCCGGC TTCAGACGAG AGGATCTTGA TCAGGAACCA 
GTGGTAAAGC GGCTTACTTC AGATCCTGTG GTCATTAAAG ATGACCATTT CAGCCAGCCC 
TTGTGTGGGA GTGACTCAAG CCACGGAGCC CTGAACTTTC CCATCCCAGA GGTGCGCTAC 
CTCATCAAGG AGATCTCTGT CATCTGGCAC CTCTACGGTG GGAAAGACTT TGGGAGCACA 
ACGTTAACAG CATCTCCTTC TAGAAGTTGG GGTTCTACAC CTCATAGCTC CCCCTCCCAG 
ACTCCGGTCC GACAATCCAA GAGTTCAAGC CGTTCTGGGG GAGGGAGAGG AAGGAACACT 
GATGTGTTGA TGGAGATACA GCACGATGTG TACCCGCAGG CTCAGGGGGC TTCTGGGCCG 
ACAGTGGATC AGCCGGTGTC TCGACAGGTG TTTGTGGTCC AGGACTTGGA GATTCGAGAC 
AGACTAGCTG CCTCACAGAT GAATAAGTTC CTTTACTTGT ATTCGAGCAA GGAAATGCCC 
AGAAAAGCTC ATTCAAATAT GTTGACAGTT AAGGCTCTCC ACATGTGTCC GGAGTCCAGC 
CAGGCTCCAC AGGAATGCTG CTTGCGTGTT TCACTCACGC CCCTTCGCCT CAATATTGAT 
CAGGATGCAC TGTTTTTCTT GAAGGACTTC TTTACCAGTC TTGCTACAGA GGTGGAGTTT 
TTCTCATCGC CATCACAAGA AGCCATCTGT GTTCCTATTA AAAAAGCAGC CACCCCAGAG 
ATCTCTTGTA GCTTCTCCAA ACACGCCAGC AGCAGCCACG ACCCAGCCCC AATCATCTCG 
CTGCCCACTC AGAGAGGTTT GAATCACAAT GGCTTCTCCA GCAGGGAGGA AATGGCTGAC 
AGTGAATCTT CTGCACCCTC GTTCACAGAC CAGCCCATAT TCTTTAGGGA GTTCAGATTT 
ACCTCTGAGG TTCCAATTCG TCTGGATTAC CATGGAAAAC ACGTGTCGAT GGAGCAGGGG 
ACCTTTGCTG GAATAATTAT GGGGTTGACA CAACTTAACT GCTCAGAGTT GAAATTGAGG 
CGCTTGTGCT ACAGACAAGG ATTATTAGGT GTGGATAAGT TGTTTTCCTA TGCAATCAAC 
GAATGGCTTA CTGACATAAA GAAGAATCAG CTTCCGGGGC TGTTGGGTGG TGTGGGGCCT 
ATTCACTCAC TGGTCCAGCT GGTTCAGGGA TTCAGGGATT TGGTCTGGCT CCCCATCGAG 
CAGTACAGGA AAGACGGTCG GATTGTTCGT GGTTTTCAGA GGGGCACTGC CTCTTTCGGC 
ACGTCGACAG CCATGGCCGC CCTGGAGCTC ACCAACAGGA TGGTGCGGAC CATCCAGGCA 
GCAGCAGAGA CAGCCTACGA CATGGTGTCT CCAGTCCCTG ATGAGAGAGA CACAAAGAGG 
GTAAAACGGT ACATCCCTTA CGGACTGGCT CATCAGCCCG TCGACCTGAG AGAGGGCGTG 
GCCAAAGCTT ACACAGTAGT AAAAGAGGGC ATTACAGACA CGGCACTGAC CATCTATGAC 
ACAGCCACCA GGGAGCACGA ACAGCGCGGG ATGACAGGGG CAGTGGGCGG AGTTCTCCGC 
CAGCTGCCGC CAGCTGTGGT CAAGCCGCTC ATCGTGGCCA CTGAGGCCAC CTCTAATGTC 
TTAGGTGGAA TGAGGAACCA GATTCACCCT GACGCAAGGC AGGAAGAGTC ACAGAAGTGG 
AGGCAGGGCG AAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011615357.1
CDS	320..6517
Translation	MPWPFSESIKKRACRYLLHRYLGNFLEEKLSLDQLSLDLYQGTGSLSQVPLDKWSLNELLETADAPFEVITGFIQTISLTVPWAALVQENCALEVKGLEMVFRPKPRVASGTEPMCWSSFMTSSMQLAKECLSQKLTDDLGESFQPFEGLEKFAETIETVLRRVKVTFLDTVLRIEHIPENSKTGIALEIRINKTVYCDETNEETSDVNVHQPTTFAHKNLQMEGITVFWDEFSEVSRAGCKSSPTPTETEPKLSPSWNPKIICEPHPQFTEPVYSAAPFEAVQVGSLGGKIKISLTLKNNLAMPGAKLDVDGHIDTLLILLSPRQVHLLLDLFGAFSGGGSQEWSKDRKSRPMQQEDEYRLHMELNRCLKKDTTVTGVDPELFESQTTRTVSSRDDVFFSMADMDMSHSLSSLPPLGEPPTVDLDLSLNSNYSASPGESPSANATALWDDYIDLPRHREKQVNESPIVLQDSQLHQNVLRQASHPSKPHGDESRPELLLNLKLSSLAISVLHIDPLPPPDAAHSPLGPMAAHFFRTVGPQHLAPAAFLESRNIFNQACPHDNLRFVAQGLKISYEHCKGSSLRTFNTDVSLNQMEFLECLFPSEGFSSDSQRGIQYTELLTFDMASPDVPLQTCLHLLYKQKERKGPQSGQFRLSTIPRKAEIQVELGPVRCELDISIVDRLNSLLQPQKLAATELMASHLYTSYNKHVSLHKAFTEVFLDDSHTPSNCHVSLTVNAPVLGLALRFPIPDLRSDQERGPWFKKSLQKEVLFLELEDLEVKTEFMAGNSPDQTKLELTFRELIGKFQQETNHPAVRFLRVSHTMDGDMTTSESVKFDWPRVVLKMNPTAVHSILERVQAEDDEGVEDHSLEEEEEEGAAHSLKDVCDFGKAEPSPFSSRRVMYENEEMVIPGDVTEMTEFQEKTMSNSRFIVELCLPNVQLSFPSKAFYETLHNRINNDLLLWEPTAPSPVETVENIPYGVGLSVASQLINTYSKDSFSQFRSSGPDDETSGSDEETMHYYIPASDPSLQSRKKKKPKAQSKTSQSLFSVIVSVNHGLIALQANAMKDKTVLKNKHGEFWLELKNGVLFSVMQHEGYKDQHYICFHTSSVCMYHQGLVDGGLSVLDVNLPSRTHPHWLDPTIYQSETSPERSSTPSECIGLEARSMVSVAVKVSSPNPEHNIKEILIAVGVRGATLQHKMAPPCTGWYDQIVDFLNITNEPVLGYAPPTSVSTLHLHLWSCSLDYRPLYLPLRSLLVVETFSISSSVSLDHSSSSLRIILDEAAVFLSDRANAVSVNLARDYVQVVDMGTLELRITAAKPGADGKLKEPRFELRCSSDIIHIRTCSDSCAALMNLIQYVASYGDLLPPAEPEPKHSSTSQRTKVEFPSRVTPQTSFLADIEQQMLQDLMSEAMEETEGQHASGLQQNGAREEHCQDHDPPRSDLFLFPDESGNLNQDLSPTCPVLHSPLITPVPNLAQEADDFCILETPGFRREDLDQEPVVKRLTSDPVVIKDDHFSQPLCGSDSSHGALNFPIPEVRYLIKEISVIWHLYGGKDFGSTTLTASPSRSWGSTPHSSPSQTPVRQSKSSSRSGGGRGRNTDVLMEIQHDVYPQAQGASGPTVDQPVSRQVFVVQDLEIRDRLAASQMNKFLYLYSSKEMPRKAHSNMLTVKALHMCPESSQAPQECCLRVSLTPLRLNIDQDALFFLKDFFTSLATEVEFFSSPSQEAICVPIKKAATPEISCSFSKHASSSHDPAPIISLPTQRGLNHNGFSSREEMADSESSAPSFTDQPIFFREFRFTSEVPIRLDYHGKHVSMEQGTFAGIIMGLTQLNCSELKLRRLCYRQGLLGVDKLFSYAINEWLTDIKKNQLPGLLGGVGPIHSLVQLVQGFRDLVWLPIEQYRKDGRIVRGFQRGTASFGTSTAMAALELTNRMVRTIQAAAETAYDMVSPVPDERDTKRVKRYIPYGLAHQPVDLREGVAKAYTVVKEGITDTALTIYDTATREHEQRGMTGAVGGVLRQLPPAVVKPLIVATEATSNVLGGMRNQIHPDARQEESQKWRQGEE

Target ORF information:

RefSeq Version	XM_011617055.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes autophagy related 2B (atg2b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011617055.1

ORF Insert Sequence:

ATGCCTTGGC CTTTTTCTGA GTCCATCAAG AAGCGGGCCT GCAGATATCT CCTGCATAGG 
TACCTTGGCA ACTTTTTGGA AGAGAAACTA AGCTTGGATC AGCTCAGTTT AGATCTCTAT 
CAAGGTACTG GCTCACTTTC GCAGGTGCCG CTTGATAAAT GGTCGCTGAA TGAACTCCTG 
GAGACAGCAG ATGCACCGTT TGAAGTCATT ACTGGGTTTA TCCAGACCAT TTCTTTAACT 
GTCCCATGGG CAGCGCTTGT GCAGGAAAAT TGTGCCCTTG AGGTCAAAGG GTTAGAGATG 
GTCTTCAGAC CAAAACCAAG AGTAGCATCT GGCACTGAGC CCATGTGCTG GTCCAGTTTT 
ATGACAAGCA GCATGCAACT GGCCAAAGAA TGCCTAAGCC AGAAGCTCAC GGATGACCTC 
GGAGAGAGTT TTCAGCCTTT TGAAGGATTA GAGAAGTTTG CCGAAACAAT AGAAACAGTC 
CTGAGGAGAG TTAAAGTGAC ATTTTTGGAT ACAGTTCTCA GAATTGAACA CATTCCAGAA 
AATTCTAAAA CTGGAATCGC GCTGGAGATT CGAATTAACA AGACGGTTTA CTGTGATGAA 
ACTAACGAGG AGACTTCAGA CGTTAACGTG CATCAGCCGA CCACGTTTGC CCACAAAAAC 
CTGCAGATGG AAGGCATCAC TGTGTTCTGG GACGAGTTCT CCGAGGTGTC CCGCGCTGGC 
TGTAAATCTT CACCAACTCC CACGGAAACC GAACCAAAAC TGTCCCCGAG CTGGAATCCT 
AAAATAATCT GCGAGCCGCA TCCCCAGTTC ACAGAACCAG TATACTCTGC AGCACCATTT 
GAAGCTGTGC AGGTCGGGAG CCTTGGTGGG AAAATCAAGA TCTCCCTCAC TCTGAAGAAC 
AACCTAGCTA TGCCCGGAGC AAAGCTGGAT GTTGACGGTC ACATCGACAC ACTGTTGATT 
CTGCTGTCGC CGCGACAAGT TCATCTTCTT TTGGACCTAT TCGGAGCCTT TTCCGGTGGA 
GGTTCACAAG AATGGAGCAA GGACAGAAAG AGCAGACCCA TGCAACAGGA GGACGAGTAC 
CGGCTCCACA TGGAGCTGAA CCGGTGTCTA AAGAAAGACA CCACGGTCAC AGGAGTCGAC 
CCTGAATTGT TTGAAAGCCA AACCACAAGA ACCGTGTCGA GTCGAGACGA CGTGTTCTTT 
TCCATGGCCG ACATGGATAT GTCTCACAGC CTGTCGTCGC TGCCTCCTCT TGGTGAACCG 
CCCACAGTCG ACTTGGATTT ATCTCTTAAC AGCAACTACT CTGCTTCTCC TGGAGAATCT 
CCCTCTGCTA ATGCAACAGC TCTGTGGGAT GATTACATTG ATCTACCTCG ACACAGAGAG 
AAGCAGGTTA ATGAAAGTCC AATTGTATTG CAAGACTCTC AACTTCATCA AAATGTATTG 
AGACAAGCGT CCCACCCTTC CAAACCTCAT GGTGATGAGT CTCGACCAGA GCTGCTTTTG 
AATCTGAAGC TGAGCAGCCT GGCCATCTCG GTCCTCCACA TCGACCCGTT GCCACCACCG 
GATGCAGCTC ACAGTCCCCT CGGTCCTATG GCTGCACACT TCTTCCGCAC AGTAGGGCCC 
CAGCATCTTG CCCCAGCGGC TTTCCTTGAG TCTCGAAACA TTTTTAACCA GGCCTGCCCC 
CATGACAATC TCAGGTTTGT GGCCCAGGGG CTCAAGATCA GCTATGAGCA CTGTAAGGGA 
TCCAGTCTGC GGACCTTCAA CACCGACGTC TCTCTCAACC AGATGGAGTT TTTAGAGTGC 
CTGTTTCCAT CTGAGGGTTT CAGCAGCGAC TCCCAAAGAG GCATTCAGTA CACAGAGCTC 
CTGACATTTG ACATGGCGAG CCCTGATGTG CCTCTGCAGA CCTGCCTTCA CCTGCTGTAC 
AAACAGAAAG AGAGGAAAGG CCCTCAGAGC GGTCAGTTTC GACTCAGCAC CATTCCCAGG 
AAAGCAGAAA TCCAGGTGGA GCTGGGCCCG GTGCGATGCG AGCTGGACAT TAGTATTGTA 
GACCGTCTCA ACTCGTTGCT CCAACCCCAG AAGCTGGCCG CCACAGAGCT GATGGCGTCT 
CACCTGTACA CATCCTATAA TAAACACGTC AGCCTGCATA AAGCCTTTAC AGAGGTTTTT 
CTGGATGACA GTCACACTCC CAGCAACTGT CACGTGTCAT TGACTGTAAA CGCGCCGGTG 
CTGGGCCTCG CTCTCCGCTT CCCTATTCCT GACCTGCGCT CAGATCAGGA ACGGGGCCCC 
TGGTTTAAGA AGTCCCTGCA GAAGGAAGTC CTCTTTCTGG AACTGGAAGA TCTGGAAGTC 
AAAACAGAAT TCATGGCTGG CAACTCACCT GATCAAACTA AGTTGGAGCT CACATTCAGG 
GAGCTCATTG GAAAATTTCA ACAGGAGACT AACCACCCAG CCGTCCGCTT CCTCCGAGTG 
TCTCACACCA TGGACGGAGA CATGACAACG TCAGAAAGTG TTAAATTTGA CTGGCCGAGG 
GTCGTGCTGA AGATGAACCC CACTGCAGTG CACTCGATAC TTGAGCGGGT GCAGGCCGAA 
GATGATGAGG GGGTAGAGGA CCATTCACTC GAAGAGGAAG AGGAGGAGGG CGCTGCACAT 
TCACTGAAGG ACGTGTGTGA CTTTGGTAAA GCAGAGCCAT CTCCGTTTTC ATCGCGAAGG 
GTCATGTACG AAAATGAGGA GATGGTCATT CCGGGTGACG TCACTGAGAT GACGGAATTC 
CAGGAGAAGA CCATGAGCAA CTCAAGGTTC ATTGTTGAGT TGTGTCTCCC CAATGTGCAG 
TTATCCTTCC CCAGCAAAGC ATTTTATGAA ACACTGCACA ACAGGATAAA TAACGATTTG 
CTGCTTTGGG AGCCGACTGC TCCATCTCCC GTGGAGACGG TGGAAAACAT CCCTTATGGA 
GTTGGCCTCT CTGTCGCCAG TCAGTTGATC AACACTTACT CCAAAGACAG CTTTAGTCAG 
TTTCGCTCCT CTGGTCCTGA TGATGAGACC AGCGGGTCAG ATGAAGAAAC CATGCATTAT 
TACATTCCTG CTTCAGACCC AAGCCTCCAG TCTCGCAAGA AGAAAAAGCC CAAAGCCCAA 
AGCAAGACCT CCCAGAGCCT ATTTTCTGTC ATCGTCAGTG TCAATCACGG TCTGATCGCG 
CTACAAGCTA ATGCTATGAA GGACAAAACT GTATTGAAAA ACAAACATGG TGAATTCTGG 
CTGGAGCTAA AGAATGGCGT GTTGTTCAGT GTCATGCAGC ACGAGGGCTA CAAAGATCAA 
CATTACATCT GTTTCCACAC CAGCAGTGTT TGCATGTATC ACCAAGGACT TGTGGATGGA 
GGCCTTTCCG TCTTGGACGT CAATTTGCCG TCCAGGACAC ATCCTCACTG GTTAGATCCA 
ACTATCTACC AATCAGAGAC TTCCCCCGAG AGATCTTCAA CGCCCTCAGA GTGCATCGGC 
CTGGAGGCTC GGAGCATGGT GTCCGTGGCC GTCAAAGTAT CATCACCGAA CCCAGAGCAC 
AACATCAAGG AAATTCTTAT TGCTGTTGGA GTGAGAGGAG CCACCTTGCA GCATAAAATG 
GCCCCTCCTT GCACGGGTTG GTACGATCAG ATTGTTGACT TTCTGAATAT CACCAATGAG 
CCAGTGTTGG GATACGCCCC TCCCACCTCG GTCTCCACCC TACACTTACA CCTGTGGAGC 
TGTTCTCTTG ACTACAGACC CCTTTATCTG CCACTCAGAT CACTGTTGGT TGTAGAGACC 
TTTAGTATCT CCAGCAGTGT GTCTTTAGAT CACTCTTCCT CATCACTCAG GATCATATTG 
GATGAGGCTG CCGTGTTCCT GTCAGACAGG GCCAATGCCG TCTCTGTCAA TCTGGCACGT 
GACTATGTGC AAGTGGTGGA CATGGGAACT CTGGAGCTCA GGATCACAGC TGCCAAACCT 
GGAGCTGACG GAAAGCTGAA AGAGCCAAGG TTCGAACTGC GCTGCTCCAG CGACATTATT 
CACATCAGAA CCTGTTCGGA CTCCTGTGCT GCCCTCATGA ACCTTATTCA GTATGTAGCC 
AGCTATGGAG ACTTACTGCC CCCAGCAGAA CCAGAGCCCA AACACAGCAG CACCTCACAG 
AGGACCAAGG TTGAATTTCC AAGTCGGGTC ACACCTCAGA CGTCGTTTCT GGCTGATATT 
GAGCAGCAGA TGTTGCAGGA TTTGATGAGC GAAGCGATGG AGGAGACAGA AGGCCAGCAT 
GCATCAGGGC TGCAGCAGAA TGGCGCACGA GAAGAACACT GTCAAGATCA CGATCCACCC 
CGCTCAGACC TGTTCTTGTT CCCGGATGAG AGCGGAAATC TTAACCAAGA TTTAAGCCCC 
ACTTGTCCCG TCCTTCACTC TCCTCTCATC ACCCCTGTTC CCAATCTGGC CCAAGAGGCT 
GATGACTTTT GTATCCTGGA GACACCCGGC TTCAGACGAG AGGATCTTGA TCAGGAACCA 
GTGGTAAAGC GGCTTACTTC AGATCCTGTG GTCATTAAAG ATGACCATTT CAGCCAGCCC 
TTGTGTGGGA GTGACTCAAG CCACGGAGCC CTGAACTTTC CCATCCCAGA GGTGCGCTAC 
CTCATCAAGG AGATCTCTGT CATCTGGCAC CTCTACGGTG GGAAAGACTT TGGGAGCACA 
ACGTTAACAG CATCTCCTTC TAGAAGTTGG GGTTCTACAC CTCATAGCTC CCCCTCCCAG 
ACTCCGGTCC GACAATCCAA GAGTTCAAGC CGTTCTGGGG GAGGGAGAGG AAGGAACACT 
GATGTGTTGA TGGAGATACA GCACGATGTG TACCCGCAGG CTCAGGGGGC TTCTGGGCCG 
ACAGTGGATC AGCCGGTGTC TCGACAGGTG TTTGTGGTCC AGGACTTGGA GATTCGAGAC 
AGACTAGCTG CCTCACAGAT GAATAAGTTC CTTTACTTGT ATTCGAGCAA GGAAATGCCC 
AGAAAAGCTC ATTCAAATAT GTTGACAGTT AAGGCTCTCC ACATGTGTCC GGAGTCCAGC 
CAGGCTCCAC AGGAATGCTG CTTGCGTGTT TCACTCACGC CCCTTCGCCT CAATATTGAT 
CAGGATGCAC TGTTTTTCTT GAAGGACTTC TTTACCAGTC TTGCTACAGA GGTGGAGTTT 
TTCTCATCGC CATCACAAGA AGCCATCTGT GTTCCTATTA AAAAAGCAGC CACCCCAGAG 
ATCTCTTGTA GCTTCTCCAA ACACGCCAGC AGCAGCCACG ACCCAGCCCC AATCATCTCG 
CTGCCCACTC AGAGAGGTTT GAATCACAAT GGCTTCTCCA GCAGGGAGGA AATGGCTGAC 
AGTGAATCTT CTGCACCCTC GTTCACAGAC CAGCCCATAT TCTTTAGGGA GTTCAGATTT 
ACCTCTGAGG TTCCAATTCG TCTGGATTAC CATGGAAAAC ACGTGTCGAT GGAGCAGGGG 
ACCTTTGCTG GAATAATTAT GGGGTTGACA CAACTTAACT GCTCAGAGTT GAAATTGAGG 
CGCTTGTGCT ACAGACAAGG ATTATTAGGT GTGGATAAGT TGTTTTCCTA TGCAATCAAC 
GAATGGCTTA CTGACATAAA GAAGAATCAG CTTCCGGGGC TGTTGGGTGG TGTGGGGCCT 
ATTCACTCAC TGGTCCAGCT GGTTCAGGGA TTCAGGGATT TGGTCTGGCT CCCCATCGAG 
CAGTACAGGA AAGACGGTCG GATTGTTCGT GGTTTTCAGA GGGGCACTGC CTCTTTCGGC 
ACGTCGACAG CCATGGCCGC CCTGGAGCTC ACCAACAGGA TGGTGCGGAC CATCCAGGCA 
GCAGCAGAGA CAGCCTACGA CATGGTGTCT CCAGTCCCTG ATGAGAGAGA CACAAAGAGG 
GTAAAACGGT ACATCCCTTA CGGACTGGCT CATCAGCCCG TCGACCTGAG AGAGGGCGTG 
GCCAAAGCTT ACACAGTAGT AAAAGAGGGC ATTACAGACA CGGCACTGAC CATCTATGAC 
ACAGCCACCA GGGAGCACGA ACAGCGCGGG ATGACAGGGG CAGTGGGCGG AGTTCTCCGC 
CAGCTGCCGC CAGCTGTGGT CAAGCCGCTC ATCGTGGCCA CTGAGGCCAC CTCTAATGTC 
TTAGGTGGAA TGAGGAACCA GATTCACCCT GACGCAAGGC AGGAAGAGTC ACAGAAGTGG 
AGGCAGGGCG AAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

atg2b ( XM_011617049.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011615351.1 Takifugu rubripes autophagy related 2B (atg2b), transcript variant X2, mRNA.

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CloneID	OTo24748
Clone ID Related Accession (Same CDS sequence)	XM_011617055.1 , XM_011617049.1
Accession Version	XM_011617049.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6198bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426348800000
Organism	Takifugu rubripes(Fugu rubripes)
Product	autophagy-related protein 2 homolog B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070921.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTGGC CTTTTTCTGA GTCCATCAAG AAGCGGGCCT GCAGATATCT CCTGCATAGG 
TACCTTGGCA ACTTTTTGGA AGAGAAACTA AGCTTGGATC AGCTCAGTTT AGATCTCTAT 
CAAGGTACTG GCTCACTTTC GCAGGTGCCG CTTGATAAAT GGTCGCTGAA TGAACTCCTG 
GAGACAGCAG ATGCACCGTT TGAAGTCATT ACTGGGTTTA TCCAGACCAT TTCTTTAACT 
GTCCCATGGG CAGCGCTTGT GCAGGAAAAT TGTGCCCTTG AGGTCAAAGG GTTAGAGATG 
GTCTTCAGAC CAAAACCAAG AGTAGCATCT GGCACTGAGC CCATGTGCTG GTCCAGTTTT 
ATGACAAGCA GCATGCAACT GGCCAAAGAA TGCCTAAGCC AGAAGCTCAC GGATGACCTC 
GGAGAGAGTT TTCAGCCTTT TGAAGGATTA GAGAAGTTTG CCGAAACAAT AGAAACAGTC 
CTGAGGAGAG TTAAAGTGAC ATTTTTGGAT ACAGTTCTCA GAATTGAACA CATTCCAGAA 
AATTCTAAAA CTGGAATCGC GCTGGAGATT CGAATTAACA AGACGGTTTA CTGTGATGAA 
ACTAACGAGG AGACTTCAGA CGTTAACGTG CATCAGCCGA CCACGTTTGC CCACAAAAAC 
CTGCAGATGG AAGGCATCAC TGTGTTCTGG GACGAGTTCT CCGAGGTGTC CCGCGCTGGC 
TGTAAATCTT CACCAACTCC CACGGAAACC GAACCAAAAC TGTCCCCGAG CTGGAATCCT 
AAAATAATCT GCGAGCCGCA TCCCCAGTTC ACAGAACCAG TATACTCTGC AGCACCATTT 
GAAGCTGTGC AGGTCGGGAG CCTTGGTGGG AAAATCAAGA TCTCCCTCAC TCTGAAGAAC 
AACCTAGCTA TGCCCGGAGC AAAGCTGGAT GTTGACGGTC ACATCGACAC ACTGTTGATT 
CTGCTGTCGC CGCGACAAGT TCATCTTCTT TTGGACCTAT TCGGAGCCTT TTCCGGTGGA 
GGTTCACAAG AATGGAGCAA GGACAGAAAG AGCAGACCCA TGCAACAGGA GGACGAGTAC 
CGGCTCCACA TGGAGCTGAA CCGGTGTCTA AAGAAAGACA CCACGGTCAC AGGAGTCGAC 
CCTGAATTGT TTGAAAGCCA AACCACAAGA ACCGTGTCGA GTCGAGACGA CGTGTTCTTT 
TCCATGGCCG ACATGGATAT GTCTCACAGC CTGTCGTCGC TGCCTCCTCT TGGTGAACCG 
CCCACAGTCG ACTTGGATTT ATCTCTTAAC AGCAACTACT CTGCTTCTCC TGGAGAATCT 
CCCTCTGCTA ATGCAACAGC TCTGTGGGAT GATTACATTG ATCTACCTCG ACACAGAGAG 
AAGCAGGTTA ATGAAAGTCC AATTGTATTG CAAGACTCTC AACTTCATCA AAATGTATTG 
AGACAAGCGT CCCACCCTTC CAAACCTCAT GGTGATGAGT CTCGACCAGA GCTGCTTTTG 
AATCTGAAGC TGAGCAGCCT GGCCATCTCG GTCCTCCACA TCGACCCGTT GCCACCACCG 
GATGCAGCTC ACAGTCCCCT CGGTCCTATG GCTGCACACT TCTTCCGCAC AGTAGGGCCC 
CAGCATCTTG CCCCAGCGGC TTTCCTTGAG TCTCGAAACA TTTTTAACCA GGCCTGCCCC 
CATGACAATC TCAGGTTTGT GGCCCAGGGG CTCAAGATCA GCTATGAGCA CTGTAAGGGA 
TCCAGTCTGC GGACCTTCAA CACCGACGTC TCTCTCAACC AGATGGAGTT TTTAGAGTGC 
CTGTTTCCAT CTGAGGGTTT CAGCAGCGAC TCCCAAAGAG GCATTCAGTA CACAGAGCTC 
CTGACATTTG ACATGGCGAG CCCTGATGTG CCTCTGCAGA CCTGCCTTCA CCTGCTGTAC 
AAACAGAAAG AGAGGAAAGG CCCTCAGAGC GGTCAGTTTC GACTCAGCAC CATTCCCAGG 
AAAGCAGAAA TCCAGGTGGA GCTGGGCCCG GTGCGATGCG AGCTGGACAT TAGTATTGTA 
GACCGTCTCA ACTCGTTGCT CCAACCCCAG AAGCTGGCCG CCACAGAGCT GATGGCGTCT 
CACCTGTACA CATCCTATAA TAAACACGTC AGCCTGCATA AAGCCTTTAC AGAGGTTTTT 
CTGGATGACA GTCACACTCC CAGCAACTGT CACGTGTCAT TGACTGTAAA CGCGCCGGTG 
CTGGGCCTCG CTCTCCGCTT CCCTATTCCT GACCTGCGCT CAGATCAGGA ACGGGGCCCC 
TGGTTTAAGA AGTCCCTGCA GAAGGAAGTC CTCTTTCTGG AACTGGAAGA TCTGGAAGTC 
AAAACAGAAT TCATGGCTGG CAACTCACCT GATCAAACTA AGTTGGAGCT CACATTCAGG 
GAGCTCATTG GAAAATTTCA ACAGGAGACT AACCACCCAG CCGTCCGCTT CCTCCGAGTG 
TCTCACACCA TGGACGGAGA CATGACAACG TCAGAAAGTG TTAAATTTGA CTGGCCGAGG 
GTCGTGCTGA AGATGAACCC CACTGCAGTG CACTCGATAC TTGAGCGGGT GCAGGCCGAA 
GATGATGAGG GGGTAGAGGA CCATTCACTC GAAGAGGAAG AGGAGGAGGG CGCTGCACAT 
TCACTGAAGG ACGTGTGTGA CTTTGGTAAA GCAGAGCCAT CTCCGTTTTC ATCGCGAAGG 
GTCATGTACG AAAATGAGGA GATGGTCATT CCGGGTGACG TCACTGAGAT GACGGAATTC 
CAGGAGAAGA CCATGAGCAA CTCAAGGTTC ATTGTTGAGT TGTGTCTCCC CAATGTGCAG 
TTATCCTTCC CCAGCAAAGC ATTTTATGAA ACACTGCACA ACAGGATAAA TAACGATTTG 
CTGCTTTGGG AGCCGACTGC TCCATCTCCC GTGGAGACGG TGGAAAACAT CCCTTATGGA 
GTTGGCCTCT CTGTCGCCAG TCAGTTGATC AACACTTACT CCAAAGACAG CTTTAGTCAG 
TTTCGCTCCT CTGGTCCTGA TGATGAGACC AGCGGGTCAG ATGAAGAAAC CATGCATTAT 
TACATTCCTG CTTCAGACCC AAGCCTCCAG TCTCGCAAGA AGAAAAAGCC CAAAGCCCAA 
AGCAAGACCT CCCAGAGCCT ATTTTCTGTC ATCGTCAGTG TCAATCACGG TCTGATCGCG 
CTACAAGCTA ATGCTATGAA GGACAAAACT GTATTGAAAA ACAAACATGG TGAATTCTGG 
CTGGAGCTAA AGAATGGCGT GTTGTTCAGT GTCATGCAGC ACGAGGGCTA CAAAGATCAA 
CATTACATCT GTTTCCACAC CAGCAGTGTT TGCATGTATC ACCAAGGACT TGTGGATGGA 
GGCCTTTCCG TCTTGGACGT CAATTTGCCG TCCAGGACAC ATCCTCACTG GTTAGATCCA 
ACTATCTACC AATCAGAGAC TTCCCCCGAG AGATCTTCAA CGCCCTCAGA GTGCATCGGC 
CTGGAGGCTC GGAGCATGGT GTCCGTGGCC GTCAAAGTAT CATCACCGAA CCCAGAGCAC 
AACATCAAGG AAATTCTTAT TGCTGTTGGA GTGAGAGGAG CCACCTTGCA GCATAAAATG 
GCCCCTCCTT GCACGGGTTG GTACGATCAG ATTGTTGACT TTCTGAATAT CACCAATGAG 
CCAGTGTTGG GATACGCCCC TCCCACCTCG GTCTCCACCC TACACTTACA CCTGTGGAGC 
TGTTCTCTTG ACTACAGACC CCTTTATCTG CCACTCAGAT CACTGTTGGT TGTAGAGACC 
TTTAGTATCT CCAGCAGTGT GTCTTTAGAT CACTCTTCCT CATCACTCAG GATCATATTG 
GATGAGGCTG CCGTGTTCCT GTCAGACAGG GCCAATGCCG TCTCTGTCAA TCTGGCACGT 
GACTATGTGC AAGTGGTGGA CATGGGAACT CTGGAGCTCA GGATCACAGC TGCCAAACCT 
GGAGCTGACG GAAAGCTGAA AGAGCCAAGG TTCGAACTGC GCTGCTCCAG CGACATTATT 
CACATCAGAA CCTGTTCGGA CTCCTGTGCT GCCCTCATGA ACCTTATTCA GTATGTAGCC 
AGCTATGGAG ACTTACTGCC CCCAGCAGAA CCAGAGCCCA AACACAGCAG CACCTCACAG 
AGGACCAAGG TTGAATTTCC AAGTCGGGTC ACACCTCAGA CGTCGTTTCT GGCTGATATT 
GAGCAGCAGA TGTTGCAGGA TTTGATGAGC GAAGCGATGG AGGAGACAGA AGGCCAGCAT 
GCATCAGGGC TGCAGCAGAA TGGCGCACGA GAAGAACACT GTCAAGATCA CGATCCACCC 
CGCTCAGACC TGTTCTTGTT CCCGGATGAG AGCGGAAATC TTAACCAAGA TTTAAGCCCC 
ACTTGTCCCG TCCTTCACTC TCCTCTCATC ACCCCTGTTC CCAATCTGGC CCAAGAGGCT 
GATGACTTTT GTATCCTGGA GACACCCGGC TTCAGACGAG AGGATCTTGA TCAGGAACCA 
GTGGTAAAGC GGCTTACTTC AGATCCTGTG GTCATTAAAG ATGACCATTT CAGCCAGCCC 
TTGTGTGGGA GTGACTCAAG CCACGGAGCC CTGAACTTTC CCATCCCAGA GGTGCGCTAC 
CTCATCAAGG AGATCTCTGT CATCTGGCAC CTCTACGGTG GGAAAGACTT TGGGAGCACA 
ACGTTAACAG CATCTCCTTC TAGAAGTTGG GGTTCTACAC CTCATAGCTC CCCCTCCCAG 
ACTCCGGTCC GACAATCCAA GAGTTCAAGC CGTTCTGGGG GAGGGAGAGG AAGGAACACT 
GATGTGTTGA TGGAGATACA GCACGATGTG TACCCGCAGG CTCAGGGGGC TTCTGGGCCG 
ACAGTGGATC AGCCGGTGTC TCGACAGGTG TTTGTGGTCC AGGACTTGGA GATTCGAGAC 
AGACTAGCTG CCTCACAGAT GAATAAGTTC CTTTACTTGT ATTCGAGCAA GGAAATGCCC 
AGAAAAGCTC ATTCAAATAT GTTGACAGTT AAGGCTCTCC ACATGTGTCC GGAGTCCAGC 
CAGGCTCCAC AGGAATGCTG CTTGCGTGTT TCACTCACGC CCCTTCGCCT CAATATTGAT 
CAGGATGCAC TGTTTTTCTT GAAGGACTTC TTTACCAGTC TTGCTACAGA GGTGGAGTTT 
TTCTCATCGC CATCACAAGA AGCCATCTGT GTTCCTATTA AAAAAGCAGC CACCCCAGAG 
ATCTCTTGTA GCTTCTCCAA ACACGCCAGC AGCAGCCACG ACCCAGCCCC AATCATCTCG 
CTGCCCACTC AGAGAGGTTT GAATCACAAT GGCTTCTCCA GCAGGGAGGA AATGGCTGAC 
AGTGAATCTT CTGCACCCTC GTTCACAGAC CAGCCCATAT TCTTTAGGGA GTTCAGATTT 
ACCTCTGAGG TTCCAATTCG TCTGGATTAC CATGGAAAAC ACGTGTCGAT GGAGCAGGGG 
ACCTTTGCTG GAATAATTAT GGGGTTGACA CAACTTAACT GCTCAGAGTT GAAATTGAGG 
CGCTTGTGCT ACAGACAAGG ATTATTAGGT GTGGATAAGT TGTTTTCCTA TGCAATCAAC 
GAATGGCTTA CTGACATAAA GAAGAATCAG CTTCCGGGGC TGTTGGGTGG TGTGGGGCCT 
ATTCACTCAC TGGTCCAGCT GGTTCAGGGA TTCAGGGATT TGGTCTGGCT CCCCATCGAG 
CAGTACAGGA AAGACGGTCG GATTGTTCGT GGTTTTCAGA GGGGCACTGC CTCTTTCGGC 
ACGTCGACAG CCATGGCCGC CCTGGAGCTC ACCAACAGGA TGGTGCGGAC CATCCAGGCA 
GCAGCAGAGA CAGCCTACGA CATGGTGTCT CCAGTCCCTG ATGAGAGAGA CACAAAGAGG 
GTAAAACGGT ACATCCCTTA CGGACTGGCT CATCAGCCCG TCGACCTGAG AGAGGGCGTG 
GCCAAAGCTT ACACAGTAGT AAAAGAGGGC ATTACAGACA CGGCACTGAC CATCTATGAC 
ACAGCCACCA GGGAGCACGA ACAGCGCGGG ATGACAGGGG CAGTGGGCGG AGTTCTCCGC 
CAGCTGCCGC CAGCTGTGGT CAAGCCGCTC ATCGTGGCCA CTGAGGCCAC CTCTAATGTC 
TTAGGTGGAA TGAGGAACCA GATTCACCCT GACGCAAGGC AGGAAGAGTC ACAGAAGTGG 
AGGCAGGGCG AAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011615351.1
CDS	320..6517
Translation	MPWPFSESIKKRACRYLLHRYLGNFLEEKLSLDQLSLDLYQGTGSLSQVPLDKWSLNELLETADAPFEVITGFIQTISLTVPWAALVQENCALEVKGLEMVFRPKPRVASGTEPMCWSSFMTSSMQLAKECLSQKLTDDLGESFQPFEGLEKFAETIETVLRRVKVTFLDTVLRIEHIPENSKTGIALEIRINKTVYCDETNEETSDVNVHQPTTFAHKNLQMEGITVFWDEFSEVSRAGCKSSPTPTETEPKLSPSWNPKIICEPHPQFTEPVYSAAPFEAVQVGSLGGKIKISLTLKNNLAMPGAKLDVDGHIDTLLILLSPRQVHLLLDLFGAFSGGGSQEWSKDRKSRPMQQEDEYRLHMELNRCLKKDTTVTGVDPELFESQTTRTVSSRDDVFFSMADMDMSHSLSSLPPLGEPPTVDLDLSLNSNYSASPGESPSANATALWDDYIDLPRHREKQVNESPIVLQDSQLHQNVLRQASHPSKPHGDESRPELLLNLKLSSLAISVLHIDPLPPPDAAHSPLGPMAAHFFRTVGPQHLAPAAFLESRNIFNQACPHDNLRFVAQGLKISYEHCKGSSLRTFNTDVSLNQMEFLECLFPSEGFSSDSQRGIQYTELLTFDMASPDVPLQTCLHLLYKQKERKGPQSGQFRLSTIPRKAEIQVELGPVRCELDISIVDRLNSLLQPQKLAATELMASHLYTSYNKHVSLHKAFTEVFLDDSHTPSNCHVSLTVNAPVLGLALRFPIPDLRSDQERGPWFKKSLQKEVLFLELEDLEVKTEFMAGNSPDQTKLELTFRELIGKFQQETNHPAVRFLRVSHTMDGDMTTSESVKFDWPRVVLKMNPTAVHSILERVQAEDDEGVEDHSLEEEEEEGAAHSLKDVCDFGKAEPSPFSSRRVMYENEEMVIPGDVTEMTEFQEKTMSNSRFIVELCLPNVQLSFPSKAFYETLHNRINNDLLLWEPTAPSPVETVENIPYGVGLSVASQLINTYSKDSFSQFRSSGPDDETSGSDEETMHYYIPASDPSLQSRKKKKPKAQSKTSQSLFSVIVSVNHGLIALQANAMKDKTVLKNKHGEFWLELKNGVLFSVMQHEGYKDQHYICFHTSSVCMYHQGLVDGGLSVLDVNLPSRTHPHWLDPTIYQSETSPERSSTPSECIGLEARSMVSVAVKVSSPNPEHNIKEILIAVGVRGATLQHKMAPPCTGWYDQIVDFLNITNEPVLGYAPPTSVSTLHLHLWSCSLDYRPLYLPLRSLLVVETFSISSSVSLDHSSSSLRIILDEAAVFLSDRANAVSVNLARDYVQVVDMGTLELRITAAKPGADGKLKEPRFELRCSSDIIHIRTCSDSCAALMNLIQYVASYGDLLPPAEPEPKHSSTSQRTKVEFPSRVTPQTSFLADIEQQMLQDLMSEAMEETEGQHASGLQQNGAREEHCQDHDPPRSDLFLFPDESGNLNQDLSPTCPVLHSPLITPVPNLAQEADDFCILETPGFRREDLDQEPVVKRLTSDPVVIKDDHFSQPLCGSDSSHGALNFPIPEVRYLIKEISVIWHLYGGKDFGSTTLTASPSRSWGSTPHSSPSQTPVRQSKSSSRSGGGRGRNTDVLMEIQHDVYPQAQGASGPTVDQPVSRQVFVVQDLEIRDRLAASQMNKFLYLYSSKEMPRKAHSNMLTVKALHMCPESSQAPQECCLRVSLTPLRLNIDQDALFFLKDFFTSLATEVEFFSSPSQEAICVPIKKAATPEISCSFSKHASSSHDPAPIISLPTQRGLNHNGFSSREEMADSESSAPSFTDQPIFFREFRFTSEVPIRLDYHGKHVSMEQGTFAGIIMGLTQLNCSELKLRRLCYRQGLLGVDKLFSYAINEWLTDIKKNQLPGLLGGVGPIHSLVQLVQGFRDLVWLPIEQYRKDGRIVRGFQRGTASFGTSTAMAALELTNRMVRTIQAAAETAYDMVSPVPDERDTKRVKRYIPYGLAHQPVDLREGVAKAYTVVKEGITDTALTIYDTATREHEQRGMTGAVGGVLRQLPPAVVKPLIVATEATSNVLGGMRNQIHPDARQEESQKWRQGEE

Target ORF information:

RefSeq Version	XM_011617049.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes autophagy related 2B (atg2b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011617049.1

ORF Insert Sequence:

ATGCCTTGGC CTTTTTCTGA GTCCATCAAG AAGCGGGCCT GCAGATATCT CCTGCATAGG 
TACCTTGGCA ACTTTTTGGA AGAGAAACTA AGCTTGGATC AGCTCAGTTT AGATCTCTAT 
CAAGGTACTG GCTCACTTTC GCAGGTGCCG CTTGATAAAT GGTCGCTGAA TGAACTCCTG 
GAGACAGCAG ATGCACCGTT TGAAGTCATT ACTGGGTTTA TCCAGACCAT TTCTTTAACT 
GTCCCATGGG CAGCGCTTGT GCAGGAAAAT TGTGCCCTTG AGGTCAAAGG GTTAGAGATG 
GTCTTCAGAC CAAAACCAAG AGTAGCATCT GGCACTGAGC CCATGTGCTG GTCCAGTTTT 
ATGACAAGCA GCATGCAACT GGCCAAAGAA TGCCTAAGCC AGAAGCTCAC GGATGACCTC 
GGAGAGAGTT TTCAGCCTTT TGAAGGATTA GAGAAGTTTG CCGAAACAAT AGAAACAGTC 
CTGAGGAGAG TTAAAGTGAC ATTTTTGGAT ACAGTTCTCA GAATTGAACA CATTCCAGAA 
AATTCTAAAA CTGGAATCGC GCTGGAGATT CGAATTAACA AGACGGTTTA CTGTGATGAA 
ACTAACGAGG AGACTTCAGA CGTTAACGTG CATCAGCCGA CCACGTTTGC CCACAAAAAC 
CTGCAGATGG AAGGCATCAC TGTGTTCTGG GACGAGTTCT CCGAGGTGTC CCGCGCTGGC 
TGTAAATCTT CACCAACTCC CACGGAAACC GAACCAAAAC TGTCCCCGAG CTGGAATCCT 
AAAATAATCT GCGAGCCGCA TCCCCAGTTC ACAGAACCAG TATACTCTGC AGCACCATTT 
GAAGCTGTGC AGGTCGGGAG CCTTGGTGGG AAAATCAAGA TCTCCCTCAC TCTGAAGAAC 
AACCTAGCTA TGCCCGGAGC AAAGCTGGAT GTTGACGGTC ACATCGACAC ACTGTTGATT 
CTGCTGTCGC CGCGACAAGT TCATCTTCTT TTGGACCTAT TCGGAGCCTT TTCCGGTGGA 
GGTTCACAAG AATGGAGCAA GGACAGAAAG AGCAGACCCA TGCAACAGGA GGACGAGTAC 
CGGCTCCACA TGGAGCTGAA CCGGTGTCTA AAGAAAGACA CCACGGTCAC AGGAGTCGAC 
CCTGAATTGT TTGAAAGCCA AACCACAAGA ACCGTGTCGA GTCGAGACGA CGTGTTCTTT 
TCCATGGCCG ACATGGATAT GTCTCACAGC CTGTCGTCGC TGCCTCCTCT TGGTGAACCG 
CCCACAGTCG ACTTGGATTT ATCTCTTAAC AGCAACTACT CTGCTTCTCC TGGAGAATCT 
CCCTCTGCTA ATGCAACAGC TCTGTGGGAT GATTACATTG ATCTACCTCG ACACAGAGAG 
AAGCAGGTTA ATGAAAGTCC AATTGTATTG CAAGACTCTC AACTTCATCA AAATGTATTG 
AGACAAGCGT CCCACCCTTC CAAACCTCAT GGTGATGAGT CTCGACCAGA GCTGCTTTTG 
AATCTGAAGC TGAGCAGCCT GGCCATCTCG GTCCTCCACA TCGACCCGTT GCCACCACCG 
GATGCAGCTC ACAGTCCCCT CGGTCCTATG GCTGCACACT TCTTCCGCAC AGTAGGGCCC 
CAGCATCTTG CCCCAGCGGC TTTCCTTGAG TCTCGAAACA TTTTTAACCA GGCCTGCCCC 
CATGACAATC TCAGGTTTGT GGCCCAGGGG CTCAAGATCA GCTATGAGCA CTGTAAGGGA 
TCCAGTCTGC GGACCTTCAA CACCGACGTC TCTCTCAACC AGATGGAGTT TTTAGAGTGC 
CTGTTTCCAT CTGAGGGTTT CAGCAGCGAC TCCCAAAGAG GCATTCAGTA CACAGAGCTC 
CTGACATTTG ACATGGCGAG CCCTGATGTG CCTCTGCAGA CCTGCCTTCA CCTGCTGTAC 
AAACAGAAAG AGAGGAAAGG CCCTCAGAGC GGTCAGTTTC GACTCAGCAC CATTCCCAGG 
AAAGCAGAAA TCCAGGTGGA GCTGGGCCCG GTGCGATGCG AGCTGGACAT TAGTATTGTA 
GACCGTCTCA ACTCGTTGCT CCAACCCCAG AAGCTGGCCG CCACAGAGCT GATGGCGTCT 
CACCTGTACA CATCCTATAA TAAACACGTC AGCCTGCATA AAGCCTTTAC AGAGGTTTTT 
CTGGATGACA GTCACACTCC CAGCAACTGT CACGTGTCAT TGACTGTAAA CGCGCCGGTG 
CTGGGCCTCG CTCTCCGCTT CCCTATTCCT GACCTGCGCT CAGATCAGGA ACGGGGCCCC 
TGGTTTAAGA AGTCCCTGCA GAAGGAAGTC CTCTTTCTGG AACTGGAAGA TCTGGAAGTC 
AAAACAGAAT TCATGGCTGG CAACTCACCT GATCAAACTA AGTTGGAGCT CACATTCAGG 
GAGCTCATTG GAAAATTTCA ACAGGAGACT AACCACCCAG CCGTCCGCTT CCTCCGAGTG 
TCTCACACCA TGGACGGAGA CATGACAACG TCAGAAAGTG TTAAATTTGA CTGGCCGAGG 
GTCGTGCTGA AGATGAACCC CACTGCAGTG CACTCGATAC TTGAGCGGGT GCAGGCCGAA 
GATGATGAGG GGGTAGAGGA CCATTCACTC GAAGAGGAAG AGGAGGAGGG CGCTGCACAT 
TCACTGAAGG ACGTGTGTGA CTTTGGTAAA GCAGAGCCAT CTCCGTTTTC ATCGCGAAGG 
GTCATGTACG AAAATGAGGA GATGGTCATT CCGGGTGACG TCACTGAGAT GACGGAATTC 
CAGGAGAAGA CCATGAGCAA CTCAAGGTTC ATTGTTGAGT TGTGTCTCCC CAATGTGCAG 
TTATCCTTCC CCAGCAAAGC ATTTTATGAA ACACTGCACA ACAGGATAAA TAACGATTTG 
CTGCTTTGGG AGCCGACTGC TCCATCTCCC GTGGAGACGG TGGAAAACAT CCCTTATGGA 
GTTGGCCTCT CTGTCGCCAG TCAGTTGATC AACACTTACT CCAAAGACAG CTTTAGTCAG 
TTTCGCTCCT CTGGTCCTGA TGATGAGACC AGCGGGTCAG ATGAAGAAAC CATGCATTAT 
TACATTCCTG CTTCAGACCC AAGCCTCCAG TCTCGCAAGA AGAAAAAGCC CAAAGCCCAA 
AGCAAGACCT CCCAGAGCCT ATTTTCTGTC ATCGTCAGTG TCAATCACGG TCTGATCGCG 
CTACAAGCTA ATGCTATGAA GGACAAAACT GTATTGAAAA ACAAACATGG TGAATTCTGG 
CTGGAGCTAA AGAATGGCGT GTTGTTCAGT GTCATGCAGC ACGAGGGCTA CAAAGATCAA 
CATTACATCT GTTTCCACAC CAGCAGTGTT TGCATGTATC ACCAAGGACT TGTGGATGGA 
GGCCTTTCCG TCTTGGACGT CAATTTGCCG TCCAGGACAC ATCCTCACTG GTTAGATCCA 
ACTATCTACC AATCAGAGAC TTCCCCCGAG AGATCTTCAA CGCCCTCAGA GTGCATCGGC 
CTGGAGGCTC GGAGCATGGT GTCCGTGGCC GTCAAAGTAT CATCACCGAA CCCAGAGCAC 
AACATCAAGG AAATTCTTAT TGCTGTTGGA GTGAGAGGAG CCACCTTGCA GCATAAAATG 
GCCCCTCCTT GCACGGGTTG GTACGATCAG ATTGTTGACT TTCTGAATAT CACCAATGAG 
CCAGTGTTGG GATACGCCCC TCCCACCTCG GTCTCCACCC TACACTTACA CCTGTGGAGC 
TGTTCTCTTG ACTACAGACC CCTTTATCTG CCACTCAGAT CACTGTTGGT TGTAGAGACC 
TTTAGTATCT CCAGCAGTGT GTCTTTAGAT CACTCTTCCT CATCACTCAG GATCATATTG 
GATGAGGCTG CCGTGTTCCT GTCAGACAGG GCCAATGCCG TCTCTGTCAA TCTGGCACGT 
GACTATGTGC AAGTGGTGGA CATGGGAACT CTGGAGCTCA GGATCACAGC TGCCAAACCT 
GGAGCTGACG GAAAGCTGAA AGAGCCAAGG TTCGAACTGC GCTGCTCCAG CGACATTATT 
CACATCAGAA CCTGTTCGGA CTCCTGTGCT GCCCTCATGA ACCTTATTCA GTATGTAGCC 
AGCTATGGAG ACTTACTGCC CCCAGCAGAA CCAGAGCCCA AACACAGCAG CACCTCACAG 
AGGACCAAGG TTGAATTTCC AAGTCGGGTC ACACCTCAGA CGTCGTTTCT GGCTGATATT 
GAGCAGCAGA TGTTGCAGGA TTTGATGAGC GAAGCGATGG AGGAGACAGA AGGCCAGCAT 
GCATCAGGGC TGCAGCAGAA TGGCGCACGA GAAGAACACT GTCAAGATCA CGATCCACCC 
CGCTCAGACC TGTTCTTGTT CCCGGATGAG AGCGGAAATC TTAACCAAGA TTTAAGCCCC 
ACTTGTCCCG TCCTTCACTC TCCTCTCATC ACCCCTGTTC CCAATCTGGC CCAAGAGGCT 
GATGACTTTT GTATCCTGGA GACACCCGGC TTCAGACGAG AGGATCTTGA TCAGGAACCA 
GTGGTAAAGC GGCTTACTTC AGATCCTGTG GTCATTAAAG ATGACCATTT CAGCCAGCCC 
TTGTGTGGGA GTGACTCAAG CCACGGAGCC CTGAACTTTC CCATCCCAGA GGTGCGCTAC 
CTCATCAAGG AGATCTCTGT CATCTGGCAC CTCTACGGTG GGAAAGACTT TGGGAGCACA 
ACGTTAACAG CATCTCCTTC TAGAAGTTGG GGTTCTACAC CTCATAGCTC CCCCTCCCAG 
ACTCCGGTCC GACAATCCAA GAGTTCAAGC CGTTCTGGGG GAGGGAGAGG AAGGAACACT 
GATGTGTTGA TGGAGATACA GCACGATGTG TACCCGCAGG CTCAGGGGGC TTCTGGGCCG 
ACAGTGGATC AGCCGGTGTC TCGACAGGTG TTTGTGGTCC AGGACTTGGA GATTCGAGAC 
AGACTAGCTG CCTCACAGAT GAATAAGTTC CTTTACTTGT ATTCGAGCAA GGAAATGCCC 
AGAAAAGCTC ATTCAAATAT GTTGACAGTT AAGGCTCTCC ACATGTGTCC GGAGTCCAGC 
CAGGCTCCAC AGGAATGCTG CTTGCGTGTT TCACTCACGC CCCTTCGCCT CAATATTGAT 
CAGGATGCAC TGTTTTTCTT GAAGGACTTC TTTACCAGTC TTGCTACAGA GGTGGAGTTT 
TTCTCATCGC CATCACAAGA AGCCATCTGT GTTCCTATTA AAAAAGCAGC CACCCCAGAG 
ATCTCTTGTA GCTTCTCCAA ACACGCCAGC AGCAGCCACG ACCCAGCCCC AATCATCTCG 
CTGCCCACTC AGAGAGGTTT GAATCACAAT GGCTTCTCCA GCAGGGAGGA AATGGCTGAC 
AGTGAATCTT CTGCACCCTC GTTCACAGAC CAGCCCATAT TCTTTAGGGA GTTCAGATTT 
ACCTCTGAGG TTCCAATTCG TCTGGATTAC CATGGAAAAC ACGTGTCGAT GGAGCAGGGG 
ACCTTTGCTG GAATAATTAT GGGGTTGACA CAACTTAACT GCTCAGAGTT GAAATTGAGG 
CGCTTGTGCT ACAGACAAGG ATTATTAGGT GTGGATAAGT TGTTTTCCTA TGCAATCAAC 
GAATGGCTTA CTGACATAAA GAAGAATCAG CTTCCGGGGC TGTTGGGTGG TGTGGGGCCT 
ATTCACTCAC TGGTCCAGCT GGTTCAGGGA TTCAGGGATT TGGTCTGGCT CCCCATCGAG 
CAGTACAGGA AAGACGGTCG GATTGTTCGT GGTTTTCAGA GGGGCACTGC CTCTTTCGGC 
ACGTCGACAG CCATGGCCGC CCTGGAGCTC ACCAACAGGA TGGTGCGGAC CATCCAGGCA 
GCAGCAGAGA CAGCCTACGA CATGGTGTCT CCAGTCCCTG ATGAGAGAGA CACAAAGAGG 
GTAAAACGGT ACATCCCTTA CGGACTGGCT CATCAGCCCG TCGACCTGAG AGAGGGCGTG 
GCCAAAGCTT ACACAGTAGT AAAAGAGGGC ATTACAGACA CGGCACTGAC CATCTATGAC 
ACAGCCACCA GGGAGCACGA ACAGCGCGGG ATGACAGGGG CAGTGGGCGG AGTTCTCCGC 
CAGCTGCCGC CAGCTGTGGT CAAGCCGCTC ATCGTGGCCA CTGAGGCCAC CTCTAATGTC 
TTAGGTGGAA TGAGGAACCA GATTCACCCT GACGCAAGGC AGGAAGAGTC ACAGAAGTGG 
AGGCAGGGCG AAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

atg2b ( XM_011617061.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011615363.1 Takifugu rubripes autophagy related 2B (atg2b), transcript variant X4, mRNA.

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CloneID	OTo24749
Clone ID Related Accession (Same CDS sequence)	XM_011617061.1
Accession Version	XM_011617061.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426348800000
Organism	Takifugu rubripes(Fugu rubripes)
Product	autophagy-related protein 2 homolog B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070921.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTGGC CTTTTTCTGA GTCCATCAAG AAGCGGGCCT GCAGATATCT CCTGCATAGG 
TACCTTGGCA ACTTTTTGGA AGAGAAACTA AGCTTGGATC AGCTCAGTTT AGATCTCTAT 
CAAGGTACTG GCTCACTTTC GCAGGTGCCG CTTGATAAAT GGTCGCTGAA TGAACTCCTG 
GAGACAGCAG ATGCACCGTT TGAAGTCATT ACTGGGTTTA TCCAGACCAT TTCTTTAACT 
GTCCCATGGG CAGCGCTTGT GCAGGAAAAT TGTGCCCTTG AGGTCAAAGG GTTAGAGATG 
GTCTTCAGAC CAAAACCAAG AGTAGCATCT GGCACTGAGC CCATGTGCTG GTCCAGTTTT 
ATGACAAGCA GCATGCAACT GGCCAAAGAA TGCCTAAGCC AGAAGCTCAC GGATGACCTC 
GGAGAGAGTT TTCAGCCTTT TGAAGGATTA GAGAAGTTTG CCGAAACAAT AGAAACAGTC 
CTGAGGAGAG TTAAAGTGAC ATTTTTGGAT ACAGTTCTCA GAATTGAACA CATTCCAGAA 
AATTCTAAAA CTGGAATCGC GCTGGAGATT CGAATTAACA AGACGGTTTA CTGTGATGAA 
ACTAACGAGG AGACTTCAGA CGTTAACGTG CATCAGCCGA CCACGTTTGC CCACAAAAAC 
CTGCAGATGG AAGGCATCAC TGTGTTCTGG GACGAGTTCT CCGAGGTGTC CCGCGCTGGC 
TGTAAATCTT CACCAACTCC CACGGAAACC GAACCAAAAC TGTCCCCGAG CTGGAATCCT 
AAAATAATCT GCGAGCCGCA TCCCCAGTTC ACAGAACCAG TATACTCTGC AGCACCATTT 
GAAGCTGTGC AGGTCGGGAG CCTTGGTGGG AAAATCAAGA TCTCCCTCAC TCTGAAGAAC 
AACCTAGCTA TGCCCGGAGC AAAGCTGGAT GTTGACGGTC ACATCGACAC ACTGTTGATT 
CTGCTGTCGC CGCGACAAGT TCATCTTCTT TTGGACCTAT TCGGAGCCTT TTCCGGTGGA 
GGTTCACAAG AATGGAGCAA GGACAGAAAG AGCAGACCCA TGCAACAGGA GGACGAGTAC 
CGGCTCCACA TGGAGCTGAA CCGGTGTCTA AAGAAAGACA CCACGGTCAC AGGAGTCGAC 
CCTGAATTGT TTGAAAGCCA AACCACAAGA ACCGTGTCGA GTCGAGACGA CGTGTTCTTT 
TCCATGGCCG ACATGGATAT GTCTCACAGC CTGTCGTCGC TGCCTCCTCT TGGTGAACCG 
CCCACAGTCG ACTTGGATTT ATCTCTTAAC AGCAACTACT CTGCTTCTCC TGGAGAATCT 
CCCTCTGCTA ATGCAACAGC TCTGTGGGAT GATTACATTG ATCTACCTCG ACACAGAGAG 
AAGCAGGTTA ATGAAAGTCC AATTGTATTG CAAGACTCTC AACTTCATCA AAATGTATTG 
AGACAAGCGT CCCACCCTTC CAAACCTCAT GGTGATGAGT CTCGACCAGA GCTGCTTTTG 
AATCTGAAGC TGAGCAGCCT GGCCATCTCG GTCCTCCACA TCGACCCGTT GCCACCACCG 
GATGCAGCTC ACAGTCCCCT CGGTCCTATG GCTGCACACT TCTTCCGCAC AGTAGGGCCC 
CAGCATCTTG CCCCAGCGGC TTTCCTTGAG TCTCGAAACA TTTTTAACCA GGCCTGCCCC 
CATGACAATC TCAGGTTTGT GGCCCAGGGG CTCAAGATCA GCTATGAGCA CTGTAAGGGA 
TCCAGTCTGC GGACCTTCAA CACCGACGTC TCTCTCAACC AGATGGAGTT TTTAGAGTGC 
CTGTTTCCAT CTGAGGGTTT CAGCAGCGAC TCCCAAAGAG GCATTCAGTA CACAGAGCTC 
CTGACATTTG ACATGGCGAG CCCTGATGTG CCTCTGCAGA CCTGCCTTCA CCTGCTGTAC 
AAACAGAAAG AGAGGAAAGG CCCTCAGAGC GGTCAGTTTC GACTCAGCAC CATTCCCAGG 
AAAGCAGAAA TCCAGGTGGA GCTGGGCCCG GTGCGATGCG AGCTGGACAT TAGTATTGTA 
GACCGTCTCA ACTCGTTGCT CCAACCCCAG AAGCTGGCCG CCACAGAGCT GATGGCGTCT 
CACCTGTACA CATCCTATAA TAAACACGTC AGCCTGCATA AAGCCTTTAC AGAGGTTTTT 
CTGGATGACA GTCACACTCC CAGCAACTGT CACGTGTCAT TGACTGTAAA CGCGCCGGTG 
CTGGGCCTCG CTCTCCGCTT CCCTATTCCT GACCTGCGCT CAGATCAGGA ACGGGGCCCC 
TGGTTTAAGA AGTCCCTGCA GAAGGAAGTC CTCTTTCTGG AACTGGAAGA TCTGGAAGTC 
AAAACAGAAT TCATGGCTGG CAACTCACCT GATCAAACTA AGTTGGAGCT CACATTCAGG 
GAGCTCATTG GAAAATTTCA ACAGGAGACT AACCACCCAG CCGTCCGCTT CCTCCGAGTG 
TCTCACACCA TGGACGGAGA CATGACAACG TCAGAAAGTG TTAAATTTGA CTGGCCGAGG 
GTCGTGCTGA AGATGAACCC CACTGCAGTG CACTCGATAC TTGAGCGGGT GCAGGCCGAA 
GATGATGAGG GGGTAGAGGA CCATTCACTC GAAGAGGAAG AGGAGGAGGG CGCTGCACAT 
TCACTGAAGG ACGTGTGTGA CTTTGGTAAA GCAGAGCCAT CTCCGTTTTC ATCGCGAAGG 
GTCATGTACG AAAATGAGGA GATGGTCATT CCGGGTGACG TCACTGAGAT GACGGAATTC 
CAGGAGAAGA CCATGAGCAA CTCAAGGTTC ATTGTTGAGT TGTGTCTCCC CAATGTGCAG 
TTATCCTTCC CCAGCAAAGC ATTTTATGAA ACACTGCACA ACAGGATAAA TAACGATTTG 
CTGCTTTGGG AGCCGACTGC TCCATCTCCC GTGGAGACGG TGGAAAACAT CCCTTATGGA 
GTTGGCCTCT CTGTCGCCAG TCAGTTGATC AACACTTACT CCAAAGACAG CTTTAGTCAG 
TTTCGCTCCT CTGGTCCTGA TGATGAGACC AGCGGGTCAG ATGAAGAAAC CATGCATTAT 
TACATTCCTG CTTCAGACCC AAGCCTCCAG TCTCGCAAGA AGAAAAAGCC CAAAGCCCAA 
AGCAAGACCT CCCAGAGCCT ATTTTCTGTC ATCGTCAGTG TCAATCACGG TCTGATCGCG 
CTACAAGCTA ATGCTATGAA GGACAAAACT GTATTGAAAA ACAAACATGG TGAATTCTGG 
CTGGAGCTAA AGAATGGCGT GTTGTTCAGT GTCATGCAGC ACGAGGGCTA CAAAGATCAA 
CATTACATCT GTTTCCACAC CAGCAGTGTT TGCATGTATC ACCAAGGACT TGTGGATGGA 
GGCCTTTCCG TCTTGGACGT CAATTTGCCG TCCAGGACAC ATCCTCACTG GTTAGATCCA 
ACTATCTACC AATCAGAGAC TTCCCCCGAG AGATCTTCAA CGCCCTCAGA GTGCATCGGC 
CTGGAGGCTC GGAGCATGGT GTCCGTGGCC GTCAAAGTAT CATCACCGAA CCCAGAGCAC 
AACATCAAGG AAATTCTTAT TGCTGTTGGA GTGAGAGGAG CCACCTTGCA GCATAAAATG 
GCCCCTCCTT GCACGGGTTG GTACGATCAG ATTGTTGACT TTCTGAATAT CACCAATGAG 
CCAGTGTTGG GATACGCCCC TCCCACCTCG GTCTCCACCC TACACTTACA CCTGTGGAGC 
TGTTCTCTTG ACTACAGACC CCTTTATCTG CCACTCAGAT CACTGTTGGT TGTAGAGACC 
TTTAGTATCT CCAGCAGTGT GTCTTTAGAT CACTCTTCCT CATCACTCAG GATCATATTG 
GATGAGGCTG CCGTGTTCCT GTCAGACAGG GCCAATGCCG TCTCTGTCAA TCTGGCACGT 
GACTATGTGC AAGTGGTGGA CATGGGAACT CTGGAGCTCA GGATCACAGC TGCCAAACCT 
GGAGCTGACG GAAAGCTGAA AGAGCCAAGG TTCGAACTGC GCTGCTCCAG CGACATTATT 
CACATCAGAA CCTGTTCGGA CTCCTGTGCT GCCCTCATGA ACCTTATTCA GTATGTAGCC 
AGCTATGGAG ACTTACTGCC CCCAGCAGAA CCAGAGCCCA AACACAGCAG CACCTCACAG 
AGGACCAAGG TTGAATTTCC AAGTCGGGTC ACACCTCAGA CGTCGTTTCT GGCTGATATT 
GAGCAGCAGA TGTTGCAGGA TTTGATGAGC GAAGCGATGG AGGAGACAGA AGGCCAGCAT 
GCATCAGGGC TGCAGCAGAA TGGCGCACGA GAAGAACACT GTCAAGATCA CGATCCACCC 
CGCTCAGACC TGTTCTTGTT CCCGGATGAG AGCGGAAATC TTAACCAAGA TTTAAGCCCC 
ACTTGTCCCG TCCTTCACTC TCCTCTCATC ACCCCTGTTC CCAATCTGGC CCAAGAGGCT 
GATGACTTTT GTATCCTGGA GACACCCGGC TTCAGACGAG AGGATCTTGA TCAGGAACCA 
GTGGTAAAGC GGCTTACTTC AGATCCTGTG GTCATTAAAG ATGACCATTT CAGCCAGCCC 
TTGTGTGGGA GTGACTCAAG CCACGGAGCC CTGAACTTTC CCATCCCAGA GGTGCGCTAC 
CTCATCAAGG AGATCTCTGT CATCTGGCAC CTCTACGGTG GGAAAGACTT TGGGAGCACA 
ACGTTAACAG CATCTCCTTC TAGAAGTTGG GGTTCTACAC CTCATAGCTC CCCCTCCCAG 
ACTCCGGTCC GACAATCCAA GAGTTCAAGC CGTTCTGGGG GAGGGAGAGG AAGGAACACT 
GATGTGTTGA TGGAGATACA GCTCAGCAAG GTGCGGTTTC AGCACGATGT GTACCCGCAG 
GCTCAGGGGG CTTCTGGGCC GACAGTGGAT CAGCCGGTGT CTCGACAGGT GTTTGTGGTC 
CAGGACTTGG AGATTCGAGA CAGACTAGCT GCCTCACAGA TGAATAAGTT CCTTTACTTG 
TATTCGAGCA AGGAAATGCC CAGAAAAGCT CATTCAAATA TGTTGACAGT TAAGGCTCTC 
CACATGTGTC CGGAGTCCAG CCAGGCTCCA CAGGAATGCT GCTTGCGTGT TTCACTCACG 
CCCCTTCGCC TCAATATTGA TCAGGATGCA CTGTTTTTCT TGAAGGACTT CTTTACCAGT 
CTTGCTACAG AGGTGGAGTT TTTCTCATCG CCATCACAAG AAGCCATCTG TGTTCCTATT 
AAAAAAGCAG CCACCCCAGA GATCTCTTGT AGCTTCTCCA AACACGCCAG CAGCAGCCAC 
GACCCAGCCC CAATCATCTC GCTGCCCACT CAGAGAGACC AGCCCATATT CTTTAGGGAG 
TTCAGATTTA CCTCTGAGGT TCCAATTCGT CTGGATTACC ATGGAAAACA CGTGTCGATG 
GAGCAGGGGA CCTTTGCTGG AATAATTATG GGGTTGACAC AACTTAACTG CTCAGAGTTG 
AAATTGAGGC GCTTGTGCTA CAGACAAGGA TTATTAGGTG TGGATAAGTT GTTTTCCTAT 
GCAATCAACG AATGGCTTAC TGACATAAAG AAGAATCAGC TTCCGGGGCT GTTGGGTGGT 
GTGGGGCCTA TTCACTCACT GGTCCAGCTG GTTCAGGGAT TCAGGGATTT GGTCTGGCTC 
CCCATCGAGC AGTACAGGAA AGACGGTCGG ATTGTTCGTG GTTTTCAGAG GGGCACTGCC 
TCTTTCGGCA CGTCGACAGC CATGGCCGCC CTGGAGCTCA CCAACAGGAT GGTGCGGACC 
ATCCAGGCAG CAGCAGAGAC AGCCTACGAC ATGGTGTCTC CAGTCCCTGA TGAGAGAGAC 
ACAAAGAGGG TAAAACGGTA CATCCCTTAC GGACTGGCTC ATCAGCCCGT CGACCTGAGA 
GAGGGCGTGG CCAAAGCTTA CACAGTAGTA AAAGAGGGCA TTACAGACAC GGCACTGACC 
ATCTATGACA CAGCCACCAG GGAGCACGAA CAGCGCGGGA TGACAGGGGC AGTGGGCGGA 
GTTCTCCGCC AGCTGCCGCC AGCTGTGGTC AAGCCGCTCA TCGTGGCCAC TGAGGCCACC 
TCTAATGTCT TAGGTGGAAT GAGGAACCAG ATTCACCCTG ACGCAAGGCA GGAAGAGTCA 
CAGAAGTGGA GGCAGGGCGA AGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011615363.1
CDS	320..6466
Translation	MPWPFSESIKKRACRYLLHRYLGNFLEEKLSLDQLSLDLYQGTGSLSQVPLDKWSLNELLETADAPFEVITGFIQTISLTVPWAALVQENCALEVKGLEMVFRPKPRVASGTEPMCWSSFMTSSMQLAKECLSQKLTDDLGESFQPFEGLEKFAETIETVLRRVKVTFLDTVLRIEHIPENSKTGIALEIRINKTVYCDETNEETSDVNVHQPTTFAHKNLQMEGITVFWDEFSEVSRAGCKSSPTPTETEPKLSPSWNPKIICEPHPQFTEPVYSAAPFEAVQVGSLGGKIKISLTLKNNLAMPGAKLDVDGHIDTLLILLSPRQVHLLLDLFGAFSGGGSQEWSKDRKSRPMQQEDEYRLHMELNRCLKKDTTVTGVDPELFESQTTRTVSSRDDVFFSMADMDMSHSLSSLPPLGEPPTVDLDLSLNSNYSASPGESPSANATALWDDYIDLPRHREKQVNESPIVLQDSQLHQNVLRQASHPSKPHGDESRPELLLNLKLSSLAISVLHIDPLPPPDAAHSPLGPMAAHFFRTVGPQHLAPAAFLESRNIFNQACPHDNLRFVAQGLKISYEHCKGSSLRTFNTDVSLNQMEFLECLFPSEGFSSDSQRGIQYTELLTFDMASPDVPLQTCLHLLYKQKERKGPQSGQFRLSTIPRKAEIQVELGPVRCELDISIVDRLNSLLQPQKLAATELMASHLYTSYNKHVSLHKAFTEVFLDDSHTPSNCHVSLTVNAPVLGLALRFPIPDLRSDQERGPWFKKSLQKEVLFLELEDLEVKTEFMAGNSPDQTKLELTFRELIGKFQQETNHPAVRFLRVSHTMDGDMTTSESVKFDWPRVVLKMNPTAVHSILERVQAEDDEGVEDHSLEEEEEEGAAHSLKDVCDFGKAEPSPFSSRRVMYENEEMVIPGDVTEMTEFQEKTMSNSRFIVELCLPNVQLSFPSKAFYETLHNRINNDLLLWEPTAPSPVETVENIPYGVGLSVASQLINTYSKDSFSQFRSSGPDDETSGSDEETMHYYIPASDPSLQSRKKKKPKAQSKTSQSLFSVIVSVNHGLIALQANAMKDKTVLKNKHGEFWLELKNGVLFSVMQHEGYKDQHYICFHTSSVCMYHQGLVDGGLSVLDVNLPSRTHPHWLDPTIYQSETSPERSSTPSECIGLEARSMVSVAVKVSSPNPEHNIKEILIAVGVRGATLQHKMAPPCTGWYDQIVDFLNITNEPVLGYAPPTSVSTLHLHLWSCSLDYRPLYLPLRSLLVVETFSISSSVSLDHSSSSLRIILDEAAVFLSDRANAVSVNLARDYVQVVDMGTLELRITAAKPGADGKLKEPRFELRCSSDIIHIRTCSDSCAALMNLIQYVASYGDLLPPAEPEPKHSSTSQRTKVEFPSRVTPQTSFLADIEQQMLQDLMSEAMEETEGQHASGLQQNGAREEHCQDHDPPRSDLFLFPDESGNLNQDLSPTCPVLHSPLITPVPNLAQEADDFCILETPGFRREDLDQEPVVKRLTSDPVVIKDDHFSQPLCGSDSSHGALNFPIPEVRYLIKEISVIWHLYGGKDFGSTTLTASPSRSWGSTPHSSPSQTPVRQSKSSSRSGGGRGRNTDVLMEIQLSKVRFQHDVYPQAQGASGPTVDQPVSRQVFVVQDLEIRDRLAASQMNKFLYLYSSKEMPRKAHSNMLTVKALHMCPESSQAPQECCLRVSLTPLRLNIDQDALFFLKDFFTSLATEVEFFSSPSQEAICVPIKKAATPEISCSFSKHASSSHDPAPIISLPTQRDQPIFFREFRFTSEVPIRLDYHGKHVSMEQGTFAGIIMGLTQLNCSELKLRRLCYRQGLLGVDKLFSYAINEWLTDIKKNQLPGLLGGVGPIHSLVQLVQGFRDLVWLPIEQYRKDGRIVRGFQRGTASFGTSTAMAALELTNRMVRTIQAAAETAYDMVSPVPDERDTKRVKRYIPYGLAHQPVDLREGVAKAYTVVKEGITDTALTIYDTATREHEQRGMTGAVGGVLRQLPPAVVKPLIVATEATSNVLGGMRNQIHPDARQEESQKWRQGEE

Target ORF information:

RefSeq Version	XM_011617061.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes autophagy related 2B (atg2b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011617061.1

ORF Insert Sequence:

ATGCCTTGGC CTTTTTCTGA GTCCATCAAG AAGCGGGCCT GCAGATATCT CCTGCATAGG 
TACCTTGGCA ACTTTTTGGA AGAGAAACTA AGCTTGGATC AGCTCAGTTT AGATCTCTAT 
CAAGGTACTG GCTCACTTTC GCAGGTGCCG CTTGATAAAT GGTCGCTGAA TGAACTCCTG 
GAGACAGCAG ATGCACCGTT TGAAGTCATT ACTGGGTTTA TCCAGACCAT TTCTTTAACT 
GTCCCATGGG CAGCGCTTGT GCAGGAAAAT TGTGCCCTTG AGGTCAAAGG GTTAGAGATG 
GTCTTCAGAC CAAAACCAAG AGTAGCATCT GGCACTGAGC CCATGTGCTG GTCCAGTTTT 
ATGACAAGCA GCATGCAACT GGCCAAAGAA TGCCTAAGCC AGAAGCTCAC GGATGACCTC 
GGAGAGAGTT TTCAGCCTTT TGAAGGATTA GAGAAGTTTG CCGAAACAAT AGAAACAGTC 
CTGAGGAGAG TTAAAGTGAC ATTTTTGGAT ACAGTTCTCA GAATTGAACA CATTCCAGAA 
AATTCTAAAA CTGGAATCGC GCTGGAGATT CGAATTAACA AGACGGTTTA CTGTGATGAA 
ACTAACGAGG AGACTTCAGA CGTTAACGTG CATCAGCCGA CCACGTTTGC CCACAAAAAC 
CTGCAGATGG AAGGCATCAC TGTGTTCTGG GACGAGTTCT CCGAGGTGTC CCGCGCTGGC 
TGTAAATCTT CACCAACTCC CACGGAAACC GAACCAAAAC TGTCCCCGAG CTGGAATCCT 
AAAATAATCT GCGAGCCGCA TCCCCAGTTC ACAGAACCAG TATACTCTGC AGCACCATTT 
GAAGCTGTGC AGGTCGGGAG CCTTGGTGGG AAAATCAAGA TCTCCCTCAC TCTGAAGAAC 
AACCTAGCTA TGCCCGGAGC AAAGCTGGAT GTTGACGGTC ACATCGACAC ACTGTTGATT 
CTGCTGTCGC CGCGACAAGT TCATCTTCTT TTGGACCTAT TCGGAGCCTT TTCCGGTGGA 
GGTTCACAAG AATGGAGCAA GGACAGAAAG AGCAGACCCA TGCAACAGGA GGACGAGTAC 
CGGCTCCACA TGGAGCTGAA CCGGTGTCTA AAGAAAGACA CCACGGTCAC AGGAGTCGAC 
CCTGAATTGT TTGAAAGCCA AACCACAAGA ACCGTGTCGA GTCGAGACGA CGTGTTCTTT 
TCCATGGCCG ACATGGATAT GTCTCACAGC CTGTCGTCGC TGCCTCCTCT TGGTGAACCG 
CCCACAGTCG ACTTGGATTT ATCTCTTAAC AGCAACTACT CTGCTTCTCC TGGAGAATCT 
CCCTCTGCTA ATGCAACAGC TCTGTGGGAT GATTACATTG ATCTACCTCG ACACAGAGAG 
AAGCAGGTTA ATGAAAGTCC AATTGTATTG CAAGACTCTC AACTTCATCA AAATGTATTG 
AGACAAGCGT CCCACCCTTC CAAACCTCAT GGTGATGAGT CTCGACCAGA GCTGCTTTTG 
AATCTGAAGC TGAGCAGCCT GGCCATCTCG GTCCTCCACA TCGACCCGTT GCCACCACCG 
GATGCAGCTC ACAGTCCCCT CGGTCCTATG GCTGCACACT TCTTCCGCAC AGTAGGGCCC 
CAGCATCTTG CCCCAGCGGC TTTCCTTGAG TCTCGAAACA TTTTTAACCA GGCCTGCCCC 
CATGACAATC TCAGGTTTGT GGCCCAGGGG CTCAAGATCA GCTATGAGCA CTGTAAGGGA 
TCCAGTCTGC GGACCTTCAA CACCGACGTC TCTCTCAACC AGATGGAGTT TTTAGAGTGC 
CTGTTTCCAT CTGAGGGTTT CAGCAGCGAC TCCCAAAGAG GCATTCAGTA CACAGAGCTC 
CTGACATTTG ACATGGCGAG CCCTGATGTG CCTCTGCAGA CCTGCCTTCA CCTGCTGTAC 
AAACAGAAAG AGAGGAAAGG CCCTCAGAGC GGTCAGTTTC GACTCAGCAC CATTCCCAGG 
AAAGCAGAAA TCCAGGTGGA GCTGGGCCCG GTGCGATGCG AGCTGGACAT TAGTATTGTA 
GACCGTCTCA ACTCGTTGCT CCAACCCCAG AAGCTGGCCG CCACAGAGCT GATGGCGTCT 
CACCTGTACA CATCCTATAA TAAACACGTC AGCCTGCATA AAGCCTTTAC AGAGGTTTTT 
CTGGATGACA GTCACACTCC CAGCAACTGT CACGTGTCAT TGACTGTAAA CGCGCCGGTG 
CTGGGCCTCG CTCTCCGCTT CCCTATTCCT GACCTGCGCT CAGATCAGGA ACGGGGCCCC 
TGGTTTAAGA AGTCCCTGCA GAAGGAAGTC CTCTTTCTGG AACTGGAAGA TCTGGAAGTC 
AAAACAGAAT TCATGGCTGG CAACTCACCT GATCAAACTA AGTTGGAGCT CACATTCAGG 
GAGCTCATTG GAAAATTTCA ACAGGAGACT AACCACCCAG CCGTCCGCTT CCTCCGAGTG 
TCTCACACCA TGGACGGAGA CATGACAACG TCAGAAAGTG TTAAATTTGA CTGGCCGAGG 
GTCGTGCTGA AGATGAACCC CACTGCAGTG CACTCGATAC TTGAGCGGGT GCAGGCCGAA 
GATGATGAGG GGGTAGAGGA CCATTCACTC GAAGAGGAAG AGGAGGAGGG CGCTGCACAT 
TCACTGAAGG ACGTGTGTGA CTTTGGTAAA GCAGAGCCAT CTCCGTTTTC ATCGCGAAGG 
GTCATGTACG AAAATGAGGA GATGGTCATT CCGGGTGACG TCACTGAGAT GACGGAATTC 
CAGGAGAAGA CCATGAGCAA CTCAAGGTTC ATTGTTGAGT TGTGTCTCCC CAATGTGCAG 
TTATCCTTCC CCAGCAAAGC ATTTTATGAA ACACTGCACA ACAGGATAAA TAACGATTTG 
CTGCTTTGGG AGCCGACTGC TCCATCTCCC GTGGAGACGG TGGAAAACAT CCCTTATGGA 
GTTGGCCTCT CTGTCGCCAG TCAGTTGATC AACACTTACT CCAAAGACAG CTTTAGTCAG 
TTTCGCTCCT CTGGTCCTGA TGATGAGACC AGCGGGTCAG ATGAAGAAAC CATGCATTAT 
TACATTCCTG CTTCAGACCC AAGCCTCCAG TCTCGCAAGA AGAAAAAGCC CAAAGCCCAA 
AGCAAGACCT CCCAGAGCCT ATTTTCTGTC ATCGTCAGTG TCAATCACGG TCTGATCGCG 
CTACAAGCTA ATGCTATGAA GGACAAAACT GTATTGAAAA ACAAACATGG TGAATTCTGG 
CTGGAGCTAA AGAATGGCGT GTTGTTCAGT GTCATGCAGC ACGAGGGCTA CAAAGATCAA 
CATTACATCT GTTTCCACAC CAGCAGTGTT TGCATGTATC ACCAAGGACT TGTGGATGGA 
GGCCTTTCCG TCTTGGACGT CAATTTGCCG TCCAGGACAC ATCCTCACTG GTTAGATCCA 
ACTATCTACC AATCAGAGAC TTCCCCCGAG AGATCTTCAA CGCCCTCAGA GTGCATCGGC 
CTGGAGGCTC GGAGCATGGT GTCCGTGGCC GTCAAAGTAT CATCACCGAA CCCAGAGCAC 
AACATCAAGG AAATTCTTAT TGCTGTTGGA GTGAGAGGAG CCACCTTGCA GCATAAAATG 
GCCCCTCCTT GCACGGGTTG GTACGATCAG ATTGTTGACT TTCTGAATAT CACCAATGAG 
CCAGTGTTGG GATACGCCCC TCCCACCTCG GTCTCCACCC TACACTTACA CCTGTGGAGC 
TGTTCTCTTG ACTACAGACC CCTTTATCTG CCACTCAGAT CACTGTTGGT TGTAGAGACC 
TTTAGTATCT CCAGCAGTGT GTCTTTAGAT CACTCTTCCT CATCACTCAG GATCATATTG 
GATGAGGCTG CCGTGTTCCT GTCAGACAGG GCCAATGCCG TCTCTGTCAA TCTGGCACGT 
GACTATGTGC AAGTGGTGGA CATGGGAACT CTGGAGCTCA GGATCACAGC TGCCAAACCT 
GGAGCTGACG GAAAGCTGAA AGAGCCAAGG TTCGAACTGC GCTGCTCCAG CGACATTATT 
CACATCAGAA CCTGTTCGGA CTCCTGTGCT GCCCTCATGA ACCTTATTCA GTATGTAGCC 
AGCTATGGAG ACTTACTGCC CCCAGCAGAA CCAGAGCCCA AACACAGCAG CACCTCACAG 
AGGACCAAGG TTGAATTTCC AAGTCGGGTC ACACCTCAGA CGTCGTTTCT GGCTGATATT 
GAGCAGCAGA TGTTGCAGGA TTTGATGAGC GAAGCGATGG AGGAGACAGA AGGCCAGCAT 
GCATCAGGGC TGCAGCAGAA TGGCGCACGA GAAGAACACT GTCAAGATCA CGATCCACCC 
CGCTCAGACC TGTTCTTGTT CCCGGATGAG AGCGGAAATC TTAACCAAGA TTTAAGCCCC 
ACTTGTCCCG TCCTTCACTC TCCTCTCATC ACCCCTGTTC CCAATCTGGC CCAAGAGGCT 
GATGACTTTT GTATCCTGGA GACACCCGGC TTCAGACGAG AGGATCTTGA TCAGGAACCA 
GTGGTAAAGC GGCTTACTTC AGATCCTGTG GTCATTAAAG ATGACCATTT CAGCCAGCCC 
TTGTGTGGGA GTGACTCAAG CCACGGAGCC CTGAACTTTC CCATCCCAGA GGTGCGCTAC 
CTCATCAAGG AGATCTCTGT CATCTGGCAC CTCTACGGTG GGAAAGACTT TGGGAGCACA 
ACGTTAACAG CATCTCCTTC TAGAAGTTGG GGTTCTACAC CTCATAGCTC CCCCTCCCAG 
ACTCCGGTCC GACAATCCAA GAGTTCAAGC CGTTCTGGGG GAGGGAGAGG AAGGAACACT 
GATGTGTTGA TGGAGATACA GCTCAGCAAG GTGCGGTTTC AGCACGATGT GTACCCGCAG 
GCTCAGGGGG CTTCTGGGCC GACAGTGGAT CAGCCGGTGT CTCGACAGGT GTTTGTGGTC 
CAGGACTTGG AGATTCGAGA CAGACTAGCT GCCTCACAGA TGAATAAGTT CCTTTACTTG 
TATTCGAGCA AGGAAATGCC CAGAAAAGCT CATTCAAATA TGTTGACAGT TAAGGCTCTC 
CACATGTGTC CGGAGTCCAG CCAGGCTCCA CAGGAATGCT GCTTGCGTGT TTCACTCACG 
CCCCTTCGCC TCAATATTGA TCAGGATGCA CTGTTTTTCT TGAAGGACTT CTTTACCAGT 
CTTGCTACAG AGGTGGAGTT TTTCTCATCG CCATCACAAG AAGCCATCTG TGTTCCTATT 
AAAAAAGCAG CCACCCCAGA GATCTCTTGT AGCTTCTCCA AACACGCCAG CAGCAGCCAC 
GACCCAGCCC CAATCATCTC GCTGCCCACT CAGAGAGACC AGCCCATATT CTTTAGGGAG 
TTCAGATTTA CCTCTGAGGT TCCAATTCGT CTGGATTACC ATGGAAAACA CGTGTCGATG 
GAGCAGGGGA CCTTTGCTGG AATAATTATG GGGTTGACAC AACTTAACTG CTCAGAGTTG 
AAATTGAGGC GCTTGTGCTA CAGACAAGGA TTATTAGGTG TGGATAAGTT GTTTTCCTAT 
GCAATCAACG AATGGCTTAC TGACATAAAG AAGAATCAGC TTCCGGGGCT GTTGGGTGGT 
GTGGGGCCTA TTCACTCACT GGTCCAGCTG GTTCAGGGAT TCAGGGATTT GGTCTGGCTC 
CCCATCGAGC AGTACAGGAA AGACGGTCGG ATTGTTCGTG GTTTTCAGAG GGGCACTGCC 
TCTTTCGGCA CGTCGACAGC CATGGCCGCC CTGGAGCTCA CCAACAGGAT GGTGCGGACC 
ATCCAGGCAG CAGCAGAGAC AGCCTACGAC ATGGTGTCTC CAGTCCCTGA TGAGAGAGAC 
ACAAAGAGGG TAAAACGGTA CATCCCTTAC GGACTGGCTC ATCAGCCCGT CGACCTGAGA 
GAGGGCGTGG CCAAAGCTTA CACAGTAGTA AAAGAGGGCA TTACAGACAC GGCACTGACC 
ATCTATGACA CAGCCACCAG GGAGCACGAA CAGCGCGGGA TGACAGGGGC AGTGGGCGGA 
GTTCTCCGCC AGCTGCCGCC AGCTGTGGTC AAGCCGCTCA TCGTGGCCAC TGAGGCCACC 
TCTAATGTCT TAGGTGGAAT GAGGAACCAG ATTCACCCTG ACGCAAGGCA GGAAGAGTCA 
CAGAAGTGGA GGCAGGGCGA AGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

atg2b ( XM_011617041.2 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011615343.2 Takifugu rubripes autophagy related 2B (atg2b), transcript variant X1, mRNA.

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CloneID	OTo73020
Clone ID Related Accession (Same CDS sequence)	XM_011617041.2 , XM_029832640.1
Accession Version	XM_011617041.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	autophagy-related protein 2 homolog B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042286.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011617041.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTGGC CTTTTTCTGA GTCCATCAAG AAGCGGGCCT GCAGATATCT CCTGCATAGG 
TACCTTGGCA ACTTTTTGGA AGAGAAACTA AGTTTGGATC AGCTCAGTTT AGATCTCTAT 
CAAGGTACTG GCTCACTTTC GCAGGTGCCG CTTGATAAAT GGTCGCTGAA TGAACTCCTG 
GAGACAGCAG ATGCACCGTT TGAAGTCATT ACTGGGTTTA TCCAGACCAT TTCTTTAACT 
GTCCCATGGG CAGCGCTTGT GCAGGAAAAT TGTGCCCTTG AGGTCAAAGG GTTAGAGATG 
GTCTTCAGAC CAAAACCAAG AGTAGCATCT GGCACTGAGC CCATGTGCTG GTCCAGTTTT 
ATGACAAGCA GCATGCAACT GGCCAAAGAA TGCCTAAGCC AGAAGCTCAC GGATGACCTC 
GGAGAGAGTT TTCAGCCTTT TGAAGGATTA GAGAAGTTTG CCGAAACAAT AGAAACAGTC 
CTGAGGAGAG TTAAAGTGAC ATTTTTGGAT ACAGTTCTCA GAATTGAACA CATTCCAGAA 
AATTCTAAAA CTGGAATCGC GCTGGAGATT CGAATTAACA AGACGGTTTA CTGTGATGAA 
ACTAACGAGG AGACTTCAGA CGTTAACGTG CATCAGCCGA CCACGTTTGC CCACAAAAAC 
CTGCAGATGG AAGGCATCAC TGTGTTCTGG GACGAGTTCT CCGAGGTGTC CCGCGCTGGC 
TGTAAATCTT CACCAACTCC CACGGAAACC GAACCAAAAC TGTCCCCGAG CTGGAATCCT 
AAAATAATCT GCGAGCCGCA TCCCCAGTTC ACAGAACCAG TATACTCTGC AGCACCATTT 
GAAGCTGTGC AGGTCGGGAG CCTTGGTGGG AAAATCAAGA TCTCCCTCAC TCTGAAGAAC 
AACCTAGCTA TGCCCGGAGC AAAGCTGGAT GTTGACGGTC ACATCGACAC ACTGTTGATT 
CTGCTGTCGC CGCGACAAGT TCATCTTCTT TTGGACCTAT TCGGAGCCTT TTCCGGTGGA 
GGTTCACAAG AATGGAGCAA GGACAGAAAG AGCAGACCCA TGCAACAGGA GGACGAGTAC 
CGGCTCCACA TGGAGCTGAA CCGGTGTCTA AAGAAAGACA CCACGGTCAC AGGAGTCGAC 
CCTGAATTGT TTGAAAGCCA AACCACAAGA ACCGTGTCGA GTCGAGACGA CGTGTTCTTT 
TCCATGGCCG ACATGGATAT GTCTCACAGC CTGTCGTCGC TGCCTCCTCT TGGTGAACCG 
CCCACAGTCG ACTTGGATTT ATCTCTTAAC AGCAACTACT CTGCTTCTCC TGGAGAATCT 
CCCTCTGCTA ATGCAACAGC TCTGTGGGAT GATTACATTG ATCTACCTCG ACACAGAGAG 
AAGCAGGTTA ATGAAAGTCC AATTGTATTG CAAGACTCTC AACTTCATCA AAATGTATTG 
AGACAAGCGT CCCACCCTTC CAAACCTCAT GGTGATGAGT CTCGACCAGA GCTGCTTTTG 
AATCTGAAGC TGAGCAGCCT GGCCATCTCG GTCCTCCACA TCGACCCGTT GCCACCACCG 
GATGCAGCTC ACAGTCCCCT CGGTCCTATG GCTGCACACT TCTTCCGCAC AGTAGGGCCC 
CAGCATCTTG CCCCAGCGGC TTTCCTTGAG TCTCGAAACA TTTTTAACCA GGCCTGCCCC 
CATGACAATC TCAGGTTTGT GGCCCAGGGG CTCAAGATCA GCTATGAGCA CTGTAAGGGA 
TCCAGTCTGC GGACCTTCAA CACCGACGTC TCTCTCAACC AGATGGAGTT TTTAGAGTGC 
CTGTTTCCAT CTGAGGGTTT CAGCAGCGAC TCCCAAAGAG GCATTCAGTA CACAGAGCTC 
CTGACATTTG ACATGGCGAG CCCTGATGTG CCTCTGCAGA CCTGCCTTCA CCTGCTGTAC 
AAACAGAAAG AGAGGAAAGG CCCTCAGAGC GGTCAGTTTC GACTCAGCAC CATTCCCAGG 
AAAGCAGAAA TCCAGGTGGA GCTGGGCCCG GTGCGATGCG AGCTGGACAT TAGTATTGTA 
GACCGTCTCA ACTCGTTGCT CCAACCCCAG AAGCTGGCCG CCACAGAGCT GATGGCGTCT 
CACCTGTACA CATCCTATAA TAAACACGTC AGCCTGCATA AAGCCTTTAC AGAGGTTTTT 
CTGGATGACA GTCACACTCC CAGCAACTGT CACGTGTCAT TGACTGTAAA CGCGCCGGTG 
CTGGGCCTCG CTCTCCGCTT CCCTATTCCT GACCTGCGCT CAGATCAGGA ACGGGGCCCC 
TGGTTTAAGA AGTCCCTGCA GAAGGAAGTC CTCTTTCTGG AACTGGAAGA TCTGGAAGTC 
AAAACAGAAT TCATGGCTGG CAACTCACCT GATCAAACTA AGTTGGAGCT CACATTCAGG 
GAGCTCATTG GAAAATTTCA ACAGGAGACT AACCACCCAG CCGTCCGCTT CCTCCGAGTG 
TCTCACACCA TGGACGGAGA CATGACAACG TCAGAAAGTG TTAAATTTGA CTGGCCGAGG 
GTCGTGCTGA AGATGAACCC CACTGCAGTG CACTCGATAC TTGAGCGGGT GCAGGCCGAA 
GATGATGAGG GGGTAGAGGA CCATTCACTC GAAGAGGAAG AGGAGGAGGG CGCTGCACAT 
TCACTGAAGG ACGTGTGTGA CTTTGGTAAA GCAGAGCCAT CTCCGTTTTC ATCGCGAAGG 
GTCATGTACG AAAATGAGGA GATGGTCATT CCGGGTGACG TCACTGAGAT GACGGAATTC 
CAGGAGAAGA CCATGAGCAA CTCAAGGTTC ATTGTTGAGT TGTGTCTCCC CAATGTGCAG 
TTATCCTTCC CCAGCAAAGC ATTTTATGAA ACACTGCACA ACAGGATAAA TAACGATTTG 
CTGCTTTGGG AGCCGACTGC TCCATCTCCC GTGGAGACGG TGGAAAACAT CCCTTATGGA 
GTTGGCCTCT CTGTCGCCAG TCAGTTGATC AACACTTACT CCAAAGACAG CTTTAGTCAG 
TTTCGCTCCT CTGGTCCTGA TGATGAGACC AGCGGGTCAG ATGAAGAAAC CATGCATTAT 
TACATTCCTG CTTCAGACCC AAGCCTCCAG TCTCGCAAGA AGAAAAAGCC CAAAGCCCAA 
AGCAAGACCT CCCAGAGCCT ATTTTCTGTC ATCGTCAGTG TCAATCACGG TCTGATCGCG 
CTACAAGCTA ATGCTATGAA GGACAAAACT GTATTGAAAA ACAAACATGG TGAATTCTGG 
CTGGAGCTAA AGAATGGCGT GTTGTTCAGT GTCATGCAGC ACGAGGGCTA CAAAGATCAA 
CATTACATCT GTTTCCACAC CAGCAGTGTT TGCATGTATC ACCAAGGACT TGTGGATGGA 
GGCCTTTCCG TCTTGGACGT CAATTTGCCG TCCAGGACAC ATCCTCACTG GTTAGATCCA 
ACTATCTACC AATCAGAGAC TTCCCCCGAG AGATCTTCAA CGCCCTCAGA GTGCATCGGC 
CTGGAGGCTC GGAGCATGGT GTCCGTGGCC GTCAAAGTAT CATCACCGAA CCCAGAGCAC 
AACATCAAGG AAATTCTTAT TGCTGTTGGA GTGAGAGGAG CCACCTTGCA GCATAAAATG 
GCCCCTCCTT GCACGGGTTG GTACGATCAG ATTGTTGACT TTCTGAATAT CACCAATGAG 
CCAGTGTTGG GATACGCCCC TCCCACCTCG GTCTCCACCC TACACTTACA CCTGTGGAGC 
TGTTCTCTTG ACTACAGACC CCTTTATCTG CCACTCAGAT CACTGTTGGT TGTAGAGACC 
TTTAGTATCT CCAGCAGTGT GTCTTTAGAT CACTCTTCCT CATCACTCAG GATCATATTG 
GATGAGGCTG CCGTGTTCCT GTCAGACAGG GCCAATGCCG TCTCTGTCAA TCTGGCACGT 
GACTATGTGC AAGTGGTGGA CATGGGAACT CTGGAGCTCA GGATCACAGC TGCCAAACCT 
GGAGCTGACG GAAAGCTGAA AGAGCCAAGG TTCGAACTGC GCTGCTCCAG CGACATTATT 
CACATCAGAA CCTGTTCGGA CTCCTGTGCT GCCCTCATGA ACCTTATTCA GTATGTAGCC 
AGCTATGGAG ACTTACTGCC CCCAGCAGAA CCAGAGCCCA AACACAGCAG TACCTCACAG 
AGGACCAAGG TTGAATTTCC AAGTCGGGTC ACACCTCAGA CGTCGTTTCT GGCTGATATT 
GAGCAGCAGA TGTTGCAGGA TTTGATGAGC GAAGCGATGG AGGAGACAGA AGGCCAGCAT 
GCATCAGGGC TGCAGCAGAA TGGCGCACGA GAAGAACACT GTCAAGATCA CGATCCACCC 
CGCTCAGACC TGTTCTTGTT CCCGGATGAG AGCGGAAATC TTAACCAAGA TTTAAGCCCC 
ACTTGTCCCG TCCTTCACTC TCCTCTCATC ACCCCTGTTC CCAATCTGGC CCAAGAGGCT 
GATGACTTTT GTATCCTGGA GACACCCGGC TTCAGACGAG AGGATCTTGA TCAGGAACCA 
GTGGTAAAGC GGCTTACTTC AGATCCTGTG GTCATTAAAG ATGACCATTT CAGCCAGCCC 
TTGTGTGGGA GTGACTCAAG CCACGGAGCC CTGAACTTTC CCATCCCAGA GGTGCGCTAC 
CTCATCAAGG AGATCTCTGT CATCTGGCAC CTCTACGGTG GGAAAGACTT TGGGAGCACA 
ACGTTAACAG CATCTCCTTC TAGAAGTTGG GGTTCTACAC CTCATAGCTC CCCCTCCCAG 
ACTCCGGTCC GACAATCCAA GAGTTCAAGC CGTTCTGGGG GAGGGAGAGG AAGGAACACT 
GATGTGTTGA TGGAGATACA GCTCAGCAAG GTGCGGTTTC AGCACGATGT GTACCCGCAG 
GCTCAGGGGG CTTCTGGGCC GACAGTGGAT CAGCCGGTGT CTCGACAGGT GTTTGTGGTC 
CAGGACTTGG AGATTCGAGA CAGACTAGCT GCCTCACAGA TGAATAAGTT CCTTTACTTG 
TATTCGAGCA AGGAAATGCC CAGAAAAGCT CATTCAAATA TGTTGACAGT TAAGGCTCTC 
CACATGTGTC CGGAGTCCAG CCAGGCTCCA CAGGAATGCT GCTTGCGTGT TTCACTCACG 
CCCCTTCGCC TCAATATTGA TCAGGATGCA CTGTTTTTCT TGAAGGACTT CTTTACCAGT 
CTTGCTACAG AGGTGGAGTT TTTCTCATCG CCATCACAAG AAGCCATCTG TGTTCCTATT 
AAAAAAGCAG CCACCCCAGA GATCTCTTGT AGCTTCTCCA AACACGCCAG CAGCAGCCAC 
GACCCAGCCC CAATCATCTC GCTGCCCACT CAGAGAGGTT TGAATCACAA TGGCTTCTCC 
AGCAGGGAGG AAATGGCTGA CAGTGAATCT TCTGCACCCT CGTTCACAGA CCAGCCCATA 
TTCTTTAGGG AGTTCAGATT TACCTCTGAG GTTCCAATTC GTCTGGATTA CCATGGAAAA 
CACGTGTCGA TGGAGCAGGG GACCTTTGCT GGAATAATTA TGGGGTTGAC ACAACTTAAC 
TGCTCAGAGT TGAAATTGAG GCGCTTGTGC TACAGACAAG GATTATTAGG TGTGGATAAG 
TTGTTTTCCT ATGCAATCAA CGAATGGCTT ACTGACATAA AGAAGAATCA GCTTCCGGGG 
CTGTTGGGTG GTGTGGGGCC TATTCACTCA CTGGTCCAGC TGGTTCAGGG ATTCAGGGAT 
TTGGTCTGGC TCCCCATCGA GCAGTACAGG AAAGACGGTC GGATTGTTCG TGGTTTTCAG 
AGGGGCACTG CCTCTTTCGG CACGTCGACA GCCATGGCCG CCCTGGAGCT CACCAACAGG 
ATGGTGCGGA CCATCCAGGC AGCAGCAGAG ACAGCCTACG ACATGGTGTC TCCAGTCCCT 
GATGAGAGAG ACACAAAGAG GGTAAAACGG TACATCCCTT ACAGACTGGC TCATCAGCCC 
GTCGACCTGA GAGAGGGCGT GGCCAAAGCT TACACAGTAG TAAAAGAGGG CATTACAGAC 
ACGGCACTGA CCATCTATGA CACAGCCACC AGGGAGCACG AACAGCGCGG GATGACAGGG 
GCAGTGGGCG GAGTTCTCCG CCAGCTGCCG CCAGCTGTGG TCAAGCCGCT CATCGTGGCC 
ACTGAGGCCA CCTCTAATGT CTTAGGTGGA ATGAGGAACC AGATTCACCC TGACGCAAGG 
CAGGAAGAGT CACAGAAGTG GAGGCAGGGC GAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011615343.2
CDS	338..6556
Translation	MPWPFSESIKKRACRYLLHRYLGNFLEEKLSLDQLSLDLYQGTGSLSQVPLDKWSLNELLETADAPFEVITGFIQTISLTVPWAALVQENCALEVKGLEMVFRPKPRVASGTEPMCWSSFMTSSMQLAKECLSQKLTDDLGESFQPFEGLEKFAETIETVLRRVKVTFLDTVLRIEHIPENSKTGIALEIRINKTVYCDETNEETSDVNVHQPTTFAHKNLQMEGITVFWDEFSEVSRAGCKSSPTPTETEPKLSPSWNPKIICEPHPQFTEPVYSAAPFEAVQVGSLGGKIKISLTLKNNLAMPGAKLDVDGHIDTLLILLSPRQVHLLLDLFGAFSGGGSQEWSKDRKSRPMQQEDEYRLHMELNRCLKKDTTVTGVDPELFESQTTRTVSSRDDVFFSMADMDMSHSLSSLPPLGEPPTVDLDLSLNSNYSASPGESPSANATALWDDYIDLPRHREKQVNESPIVLQDSQLHQNVLRQASHPSKPHGDESRPELLLNLKLSSLAISVLHIDPLPPPDAAHSPLGPMAAHFFRTVGPQHLAPAAFLESRNIFNQACPHDNLRFVAQGLKISYEHCKGSSLRTFNTDVSLNQMEFLECLFPSEGFSSDSQRGIQYTELLTFDMASPDVPLQTCLHLLYKQKERKGPQSGQFRLSTIPRKAEIQVELGPVRCELDISIVDRLNSLLQPQKLAATELMASHLYTSYNKHVSLHKAFTEVFLDDSHTPSNCHVSLTVNAPVLGLALRFPIPDLRSDQERGPWFKKSLQKEVLFLELEDLEVKTEFMAGNSPDQTKLELTFRELIGKFQQETNHPAVRFLRVSHTMDGDMTTSESVKFDWPRVVLKMNPTAVHSILERVQAEDDEGVEDHSLEEEEEEGAAHSLKDVCDFGKAEPSPFSSRRVMYENEEMVIPGDVTEMTEFQEKTMSNSRFIVELCLPNVQLSFPSKAFYETLHNRINNDLLLWEPTAPSPVETVENIPYGVGLSVASQLINTYSKDSFSQFRSSGPDDETSGSDEETMHYYIPASDPSLQSRKKKKPKAQSKTSQSLFSVIVSVNHGLIALQANAMKDKTVLKNKHGEFWLELKNGVLFSVMQHEGYKDQHYICFHTSSVCMYHQGLVDGGLSVLDVNLPSRTHPHWLDPTIYQSETSPERSSTPSECIGLEARSMVSVAVKVSSPNPEHNIKEILIAVGVRGATLQHKMAPPCTGWYDQIVDFLNITNEPVLGYAPPTSVSTLHLHLWSCSLDYRPLYLPLRSLLVVETFSISSSVSLDHSSSSLRIILDEAAVFLSDRANAVSVNLARDYVQVVDMGTLELRITAAKPGADGKLKEPRFELRCSSDIIHIRTCSDSCAALMNLIQYVASYGDLLPPAEPEPKHSSTSQRTKVEFPSRVTPQTSFLADIEQQMLQDLMSEAMEETEGQHASGLQQNGAREEHCQDHDPPRSDLFLFPDESGNLNQDLSPTCPVLHSPLITPVPNLAQEADDFCILETPGFRREDLDQEPVVKRLTSDPVVIKDDHFSQPLCGSDSSHGALNFPIPEVRYLIKEISVIWHLYGGKDFGSTTLTASPSRSWGSTPHSSPSQTPVRQSKSSSRSGGGRGRNTDVLMEIQLSKVRFQHDVYPQAQGASGPTVDQPVSRQVFVVQDLEIRDRLAASQMNKFLYLYSSKEMPRKAHSNMLTVKALHMCPESSQAPQECCLRVSLTPLRLNIDQDALFFLKDFFTSLATEVEFFSSPSQEAICVPIKKAATPEISCSFSKHASSSHDPAPIISLPTQRGLNHNGFSSREEMADSESSAPSFTDQPIFFREFRFTSEVPIRLDYHGKHVSMEQGTFAGIIMGLTQLNCSELKLRRLCYRQGLLGVDKLFSYAINEWLTDIKKNQLPGLLGGVGPIHSLVQLVQGFRDLVWLPIEQYRKDGRIVRGFQRGTASFGTSTAMAALELTNRMVRTIQAAAETAYDMVSPVPDERDTKRVKRYIPYRLAHQPVDLREGVAKAYTVVKEGITDTALTIYDTATREHEQRGMTGAVGGVLRQLPPAVVKPLIVATEATSNVLGGMRNQIHPDARQEESQKWRQGEE

Target ORF information:

RefSeq Version	XM_011617041.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes autophagy related 2B (atg2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011617041.2

ORF Insert Sequence:

ATGCCTTGGC CTTTTTCTGA GTCCATCAAG AAGCGGGCCT GCAGATATCT CCTGCATAGG 
TACCTTGGCA ACTTTTTGGA AGAGAAACTA AGTTTGGATC AGCTCAGTTT AGATCTCTAT 
CAAGGTACTG GCTCACTTTC GCAGGTGCCG CTTGATAAAT GGTCGCTGAA TGAACTCCTG 
GAGACAGCAG ATGCACCGTT TGAAGTCATT ACTGGGTTTA TCCAGACCAT TTCTTTAACT 
GTCCCATGGG CAGCGCTTGT GCAGGAAAAT TGTGCCCTTG AGGTCAAAGG GTTAGAGATG 
GTCTTCAGAC CAAAACCAAG AGTAGCATCT GGCACTGAGC CCATGTGCTG GTCCAGTTTT 
ATGACAAGCA GCATGCAACT GGCCAAAGAA TGCCTAAGCC AGAAGCTCAC GGATGACCTC 
GGAGAGAGTT TTCAGCCTTT TGAAGGATTA GAGAAGTTTG CCGAAACAAT AGAAACAGTC 
CTGAGGAGAG TTAAAGTGAC ATTTTTGGAT ACAGTTCTCA GAATTGAACA CATTCCAGAA 
AATTCTAAAA CTGGAATCGC GCTGGAGATT CGAATTAACA AGACGGTTTA CTGTGATGAA 
ACTAACGAGG AGACTTCAGA CGTTAACGTG CATCAGCCGA CCACGTTTGC CCACAAAAAC 
CTGCAGATGG AAGGCATCAC TGTGTTCTGG GACGAGTTCT CCGAGGTGTC CCGCGCTGGC 
TGTAAATCTT CACCAACTCC CACGGAAACC GAACCAAAAC TGTCCCCGAG CTGGAATCCT 
AAAATAATCT GCGAGCCGCA TCCCCAGTTC ACAGAACCAG TATACTCTGC AGCACCATTT 
GAAGCTGTGC AGGTCGGGAG CCTTGGTGGG AAAATCAAGA TCTCCCTCAC TCTGAAGAAC 
AACCTAGCTA TGCCCGGAGC AAAGCTGGAT GTTGACGGTC ACATCGACAC ACTGTTGATT 
CTGCTGTCGC CGCGACAAGT TCATCTTCTT TTGGACCTAT TCGGAGCCTT TTCCGGTGGA 
GGTTCACAAG AATGGAGCAA GGACAGAAAG AGCAGACCCA TGCAACAGGA GGACGAGTAC 
CGGCTCCACA TGGAGCTGAA CCGGTGTCTA AAGAAAGACA CCACGGTCAC AGGAGTCGAC 
CCTGAATTGT TTGAAAGCCA AACCACAAGA ACCGTGTCGA GTCGAGACGA CGTGTTCTTT 
TCCATGGCCG ACATGGATAT GTCTCACAGC CTGTCGTCGC TGCCTCCTCT TGGTGAACCG 
CCCACAGTCG ACTTGGATTT ATCTCTTAAC AGCAACTACT CTGCTTCTCC TGGAGAATCT 
CCCTCTGCTA ATGCAACAGC TCTGTGGGAT GATTACATTG ATCTACCTCG ACACAGAGAG 
AAGCAGGTTA ATGAAAGTCC AATTGTATTG CAAGACTCTC AACTTCATCA AAATGTATTG 
AGACAAGCGT CCCACCCTTC CAAACCTCAT GGTGATGAGT CTCGACCAGA GCTGCTTTTG 
AATCTGAAGC TGAGCAGCCT GGCCATCTCG GTCCTCCACA TCGACCCGTT GCCACCACCG 
GATGCAGCTC ACAGTCCCCT CGGTCCTATG GCTGCACACT TCTTCCGCAC AGTAGGGCCC 
CAGCATCTTG CCCCAGCGGC TTTCCTTGAG TCTCGAAACA TTTTTAACCA GGCCTGCCCC 
CATGACAATC TCAGGTTTGT GGCCCAGGGG CTCAAGATCA GCTATGAGCA CTGTAAGGGA 
TCCAGTCTGC GGACCTTCAA CACCGACGTC TCTCTCAACC AGATGGAGTT TTTAGAGTGC 
CTGTTTCCAT CTGAGGGTTT CAGCAGCGAC TCCCAAAGAG GCATTCAGTA CACAGAGCTC 
CTGACATTTG ACATGGCGAG CCCTGATGTG CCTCTGCAGA CCTGCCTTCA CCTGCTGTAC 
AAACAGAAAG AGAGGAAAGG CCCTCAGAGC GGTCAGTTTC GACTCAGCAC CATTCCCAGG 
AAAGCAGAAA TCCAGGTGGA GCTGGGCCCG GTGCGATGCG AGCTGGACAT TAGTATTGTA 
GACCGTCTCA ACTCGTTGCT CCAACCCCAG AAGCTGGCCG CCACAGAGCT GATGGCGTCT 
CACCTGTACA CATCCTATAA TAAACACGTC AGCCTGCATA AAGCCTTTAC AGAGGTTTTT 
CTGGATGACA GTCACACTCC CAGCAACTGT CACGTGTCAT TGACTGTAAA CGCGCCGGTG 
CTGGGCCTCG CTCTCCGCTT CCCTATTCCT GACCTGCGCT CAGATCAGGA ACGGGGCCCC 
TGGTTTAAGA AGTCCCTGCA GAAGGAAGTC CTCTTTCTGG AACTGGAAGA TCTGGAAGTC 
AAAACAGAAT TCATGGCTGG CAACTCACCT GATCAAACTA AGTTGGAGCT CACATTCAGG 
GAGCTCATTG GAAAATTTCA ACAGGAGACT AACCACCCAG CCGTCCGCTT CCTCCGAGTG 
TCTCACACCA TGGACGGAGA CATGACAACG TCAGAAAGTG TTAAATTTGA CTGGCCGAGG 
GTCGTGCTGA AGATGAACCC CACTGCAGTG CACTCGATAC TTGAGCGGGT GCAGGCCGAA 
GATGATGAGG GGGTAGAGGA CCATTCACTC GAAGAGGAAG AGGAGGAGGG CGCTGCACAT 
TCACTGAAGG ACGTGTGTGA CTTTGGTAAA GCAGAGCCAT CTCCGTTTTC ATCGCGAAGG 
GTCATGTACG AAAATGAGGA GATGGTCATT CCGGGTGACG TCACTGAGAT GACGGAATTC 
CAGGAGAAGA CCATGAGCAA CTCAAGGTTC ATTGTTGAGT TGTGTCTCCC CAATGTGCAG 
TTATCCTTCC CCAGCAAAGC ATTTTATGAA ACACTGCACA ACAGGATAAA TAACGATTTG 
CTGCTTTGGG AGCCGACTGC TCCATCTCCC GTGGAGACGG TGGAAAACAT CCCTTATGGA 
GTTGGCCTCT CTGTCGCCAG TCAGTTGATC AACACTTACT CCAAAGACAG CTTTAGTCAG 
TTTCGCTCCT CTGGTCCTGA TGATGAGACC AGCGGGTCAG ATGAAGAAAC CATGCATTAT 
TACATTCCTG CTTCAGACCC AAGCCTCCAG TCTCGCAAGA AGAAAAAGCC CAAAGCCCAA 
AGCAAGACCT CCCAGAGCCT ATTTTCTGTC ATCGTCAGTG TCAATCACGG TCTGATCGCG 
CTACAAGCTA ATGCTATGAA GGACAAAACT GTATTGAAAA ACAAACATGG TGAATTCTGG 
CTGGAGCTAA AGAATGGCGT GTTGTTCAGT GTCATGCAGC ACGAGGGCTA CAAAGATCAA 
CATTACATCT GTTTCCACAC CAGCAGTGTT TGCATGTATC ACCAAGGACT TGTGGATGGA 
GGCCTTTCCG TCTTGGACGT CAATTTGCCG TCCAGGACAC ATCCTCACTG GTTAGATCCA 
ACTATCTACC AATCAGAGAC TTCCCCCGAG AGATCTTCAA CGCCCTCAGA GTGCATCGGC 
CTGGAGGCTC GGAGCATGGT GTCCGTGGCC GTCAAAGTAT CATCACCGAA CCCAGAGCAC 
AACATCAAGG AAATTCTTAT TGCTGTTGGA GTGAGAGGAG CCACCTTGCA GCATAAAATG 
GCCCCTCCTT GCACGGGTTG GTACGATCAG ATTGTTGACT TTCTGAATAT CACCAATGAG 
CCAGTGTTGG GATACGCCCC TCCCACCTCG GTCTCCACCC TACACTTACA CCTGTGGAGC 
TGTTCTCTTG ACTACAGACC CCTTTATCTG CCACTCAGAT CACTGTTGGT TGTAGAGACC 
TTTAGTATCT CCAGCAGTGT GTCTTTAGAT CACTCTTCCT CATCACTCAG GATCATATTG 
GATGAGGCTG CCGTGTTCCT GTCAGACAGG GCCAATGCCG TCTCTGTCAA TCTGGCACGT 
GACTATGTGC AAGTGGTGGA CATGGGAACT CTGGAGCTCA GGATCACAGC TGCCAAACCT 
GGAGCTGACG GAAAGCTGAA AGAGCCAAGG TTCGAACTGC GCTGCTCCAG CGACATTATT 
CACATCAGAA CCTGTTCGGA CTCCTGTGCT GCCCTCATGA ACCTTATTCA GTATGTAGCC 
AGCTATGGAG ACTTACTGCC CCCAGCAGAA CCAGAGCCCA AACACAGCAG TACCTCACAG 
AGGACCAAGG TTGAATTTCC AAGTCGGGTC ACACCTCAGA CGTCGTTTCT GGCTGATATT 
GAGCAGCAGA TGTTGCAGGA TTTGATGAGC GAAGCGATGG AGGAGACAGA AGGCCAGCAT 
GCATCAGGGC TGCAGCAGAA TGGCGCACGA GAAGAACACT GTCAAGATCA CGATCCACCC 
CGCTCAGACC TGTTCTTGTT CCCGGATGAG AGCGGAAATC TTAACCAAGA TTTAAGCCCC 
ACTTGTCCCG TCCTTCACTC TCCTCTCATC ACCCCTGTTC CCAATCTGGC CCAAGAGGCT 
GATGACTTTT GTATCCTGGA GACACCCGGC TTCAGACGAG AGGATCTTGA TCAGGAACCA 
GTGGTAAAGC GGCTTACTTC AGATCCTGTG GTCATTAAAG ATGACCATTT CAGCCAGCCC 
TTGTGTGGGA GTGACTCAAG CCACGGAGCC CTGAACTTTC CCATCCCAGA GGTGCGCTAC 
CTCATCAAGG AGATCTCTGT CATCTGGCAC CTCTACGGTG GGAAAGACTT TGGGAGCACA 
ACGTTAACAG CATCTCCTTC TAGAAGTTGG GGTTCTACAC CTCATAGCTC CCCCTCCCAG 
ACTCCGGTCC GACAATCCAA GAGTTCAAGC CGTTCTGGGG GAGGGAGAGG AAGGAACACT 
GATGTGTTGA TGGAGATACA GCTCAGCAAG GTGCGGTTTC AGCACGATGT GTACCCGCAG 
GCTCAGGGGG CTTCTGGGCC GACAGTGGAT CAGCCGGTGT CTCGACAGGT GTTTGTGGTC 
CAGGACTTGG AGATTCGAGA CAGACTAGCT GCCTCACAGA TGAATAAGTT CCTTTACTTG 
TATTCGAGCA AGGAAATGCC CAGAAAAGCT CATTCAAATA TGTTGACAGT TAAGGCTCTC 
CACATGTGTC CGGAGTCCAG CCAGGCTCCA CAGGAATGCT GCTTGCGTGT TTCACTCACG 
CCCCTTCGCC TCAATATTGA TCAGGATGCA CTGTTTTTCT TGAAGGACTT CTTTACCAGT 
CTTGCTACAG AGGTGGAGTT TTTCTCATCG CCATCACAAG AAGCCATCTG TGTTCCTATT 
AAAAAAGCAG CCACCCCAGA GATCTCTTGT AGCTTCTCCA AACACGCCAG CAGCAGCCAC 
GACCCAGCCC CAATCATCTC GCTGCCCACT CAGAGAGGTT TGAATCACAA TGGCTTCTCC 
AGCAGGGAGG AAATGGCTGA CAGTGAATCT TCTGCACCCT CGTTCACAGA CCAGCCCATA 
TTCTTTAGGG AGTTCAGATT TACCTCTGAG GTTCCAATTC GTCTGGATTA CCATGGAAAA 
CACGTGTCGA TGGAGCAGGG GACCTTTGCT GGAATAATTA TGGGGTTGAC ACAACTTAAC 
TGCTCAGAGT TGAAATTGAG GCGCTTGTGC TACAGACAAG GATTATTAGG TGTGGATAAG 
TTGTTTTCCT ATGCAATCAA CGAATGGCTT ACTGACATAA AGAAGAATCA GCTTCCGGGG 
CTGTTGGGTG GTGTGGGGCC TATTCACTCA CTGGTCCAGC TGGTTCAGGG ATTCAGGGAT 
TTGGTCTGGC TCCCCATCGA GCAGTACAGG AAAGACGGTC GGATTGTTCG TGGTTTTCAG 
AGGGGCACTG CCTCTTTCGG CACGTCGACA GCCATGGCCG CCCTGGAGCT CACCAACAGG 
ATGGTGCGGA CCATCCAGGC AGCAGCAGAG ACAGCCTACG ACATGGTGTC TCCAGTCCCT 
GATGAGAGAG ACACAAAGAG GGTAAAACGG TACATCCCTT ACAGACTGGC TCATCAGCCC 
GTCGACCTGA GAGAGGGCGT GGCCAAAGCT TACACAGTAG TAAAAGAGGG CATTACAGAC 
ACGGCACTGA CCATCTATGA CACAGCCACC AGGGAGCACG AACAGCGCGG GATGACAGGG 
GCAGTGGGCG GAGTTCTCCG CCAGCTGCCG CCAGCTGTGG TCAAGCCGCT CATCGTGGCC 
ACTGAGGCCA CCTCTAATGT CTTAGGTGGA ATGAGGAACC AGATTCACCC TGACGCAAGG 
CAGGAAGAGT CACAGAAGTG GAGGCAGGGC GAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

atg2b ( XM_029832640.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_029688500.1 Takifugu rubripes autophagy related 2B (atg2b), transcript variant X2, mRNA.

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CloneID	OTo73020
Clone ID Related Accession (Same CDS sequence)	XM_011617041.2 , XM_029832640.1
Accession Version	XM_029832640.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	autophagy-related protein 2 homolog B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042286.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTGGC CTTTTTCTGA GTCCATCAAG AAGCGGGCCT GCAGATATCT CCTGCATAGG 
TACCTTGGCA ACTTTTTGGA AGAGAAACTA AGTTTGGATC AGCTCAGTTT AGATCTCTAT 
CAAGGTACTG GCTCACTTTC GCAGGTGCCG CTTGATAAAT GGTCGCTGAA TGAACTCCTG 
GAGACAGCAG ATGCACCGTT TGAAGTCATT ACTGGGTTTA TCCAGACCAT TTCTTTAACT 
GTCCCATGGG CAGCGCTTGT GCAGGAAAAT TGTGCCCTTG AGGTCAAAGG GTTAGAGATG 
GTCTTCAGAC CAAAACCAAG AGTAGCATCT GGCACTGAGC CCATGTGCTG GTCCAGTTTT 
ATGACAAGCA GCATGCAACT GGCCAAAGAA TGCCTAAGCC AGAAGCTCAC GGATGACCTC 
GGAGAGAGTT TTCAGCCTTT TGAAGGATTA GAGAAGTTTG CCGAAACAAT AGAAACAGTC 
CTGAGGAGAG TTAAAGTGAC ATTTTTGGAT ACAGTTCTCA GAATTGAACA CATTCCAGAA 
AATTCTAAAA CTGGAATCGC GCTGGAGATT CGAATTAACA AGACGGTTTA CTGTGATGAA 
ACTAACGAGG AGACTTCAGA CGTTAACGTG CATCAGCCGA CCACGTTTGC CCACAAAAAC 
CTGCAGATGG AAGGCATCAC TGTGTTCTGG GACGAGTTCT CCGAGGTGTC CCGCGCTGGC 
TGTAAATCTT CACCAACTCC CACGGAAACC GAACCAAAAC TGTCCCCGAG CTGGAATCCT 
AAAATAATCT GCGAGCCGCA TCCCCAGTTC ACAGAACCAG TATACTCTGC AGCACCATTT 
GAAGCTGTGC AGGTCGGGAG CCTTGGTGGG AAAATCAAGA TCTCCCTCAC TCTGAAGAAC 
AACCTAGCTA TGCCCGGAGC AAAGCTGGAT GTTGACGGTC ACATCGACAC ACTGTTGATT 
CTGCTGTCGC CGCGACAAGT TCATCTTCTT TTGGACCTAT TCGGAGCCTT TTCCGGTGGA 
GGTTCACAAG AATGGAGCAA GGACAGAAAG AGCAGACCCA TGCAACAGGA GGACGAGTAC 
CGGCTCCACA TGGAGCTGAA CCGGTGTCTA AAGAAAGACA CCACGGTCAC AGGAGTCGAC 
CCTGAATTGT TTGAAAGCCA AACCACAAGA ACCGTGTCGA GTCGAGACGA CGTGTTCTTT 
TCCATGGCCG ACATGGATAT GTCTCACAGC CTGTCGTCGC TGCCTCCTCT TGGTGAACCG 
CCCACAGTCG ACTTGGATTT ATCTCTTAAC AGCAACTACT CTGCTTCTCC TGGAGAATCT 
CCCTCTGCTA ATGCAACAGC TCTGTGGGAT GATTACATTG ATCTACCTCG ACACAGAGAG 
AAGCAGGTTA ATGAAAGTCC AATTGTATTG CAAGACTCTC AACTTCATCA AAATGTATTG 
AGACAAGCGT CCCACCCTTC CAAACCTCAT GGTGATGAGT CTCGACCAGA GCTGCTTTTG 
AATCTGAAGC TGAGCAGCCT GGCCATCTCG GTCCTCCACA TCGACCCGTT GCCACCACCG 
GATGCAGCTC ACAGTCCCCT CGGTCCTATG GCTGCACACT TCTTCCGCAC AGTAGGGCCC 
CAGCATCTTG CCCCAGCGGC TTTCCTTGAG TCTCGAAACA TTTTTAACCA GGCCTGCCCC 
CATGACAATC TCAGGTTTGT GGCCCAGGGG CTCAAGATCA GCTATGAGCA CTGTAAGGGA 
TCCAGTCTGC GGACCTTCAA CACCGACGTC TCTCTCAACC AGATGGAGTT TTTAGAGTGC 
CTGTTTCCAT CTGAGGGTTT CAGCAGCGAC TCCCAAAGAG GCATTCAGTA CACAGAGCTC 
CTGACATTTG ACATGGCGAG CCCTGATGTG CCTCTGCAGA CCTGCCTTCA CCTGCTGTAC 
AAACAGAAAG AGAGGAAAGG CCCTCAGAGC GGTCAGTTTC GACTCAGCAC CATTCCCAGG 
AAAGCAGAAA TCCAGGTGGA GCTGGGCCCG GTGCGATGCG AGCTGGACAT TAGTATTGTA 
GACCGTCTCA ACTCGTTGCT CCAACCCCAG AAGCTGGCCG CCACAGAGCT GATGGCGTCT 
CACCTGTACA CATCCTATAA TAAACACGTC AGCCTGCATA AAGCCTTTAC AGAGGTTTTT 
CTGGATGACA GTCACACTCC CAGCAACTGT CACGTGTCAT TGACTGTAAA CGCGCCGGTG 
CTGGGCCTCG CTCTCCGCTT CCCTATTCCT GACCTGCGCT CAGATCAGGA ACGGGGCCCC 
TGGTTTAAGA AGTCCCTGCA GAAGGAAGTC CTCTTTCTGG AACTGGAAGA TCTGGAAGTC 
AAAACAGAAT TCATGGCTGG CAACTCACCT GATCAAACTA AGTTGGAGCT CACATTCAGG 
GAGCTCATTG GAAAATTTCA ACAGGAGACT AACCACCCAG CCGTCCGCTT CCTCCGAGTG 
TCTCACACCA TGGACGGAGA CATGACAACG TCAGAAAGTG TTAAATTTGA CTGGCCGAGG 
GTCGTGCTGA AGATGAACCC CACTGCAGTG CACTCGATAC TTGAGCGGGT GCAGGCCGAA 
GATGATGAGG GGGTAGAGGA CCATTCACTC GAAGAGGAAG AGGAGGAGGG CGCTGCACAT 
TCACTGAAGG ACGTGTGTGA CTTTGGTAAA GCAGAGCCAT CTCCGTTTTC ATCGCGAAGG 
GTCATGTACG AAAATGAGGA GATGGTCATT CCGGGTGACG TCACTGAGAT GACGGAATTC 
CAGGAGAAGA CCATGAGCAA CTCAAGGTTC ATTGTTGAGT TGTGTCTCCC CAATGTGCAG 
TTATCCTTCC CCAGCAAAGC ATTTTATGAA ACACTGCACA ACAGGATAAA TAACGATTTG 
CTGCTTTGGG AGCCGACTGC TCCATCTCCC GTGGAGACGG TGGAAAACAT CCCTTATGGA 
GTTGGCCTCT CTGTCGCCAG TCAGTTGATC AACACTTACT CCAAAGACAG CTTTAGTCAG 
TTTCGCTCCT CTGGTCCTGA TGATGAGACC AGCGGGTCAG ATGAAGAAAC CATGCATTAT 
TACATTCCTG CTTCAGACCC AAGCCTCCAG TCTCGCAAGA AGAAAAAGCC CAAAGCCCAA 
AGCAAGACCT CCCAGAGCCT ATTTTCTGTC ATCGTCAGTG TCAATCACGG TCTGATCGCG 
CTACAAGCTA ATGCTATGAA GGACAAAACT GTATTGAAAA ACAAACATGG TGAATTCTGG 
CTGGAGCTAA AGAATGGCGT GTTGTTCAGT GTCATGCAGC ACGAGGGCTA CAAAGATCAA 
CATTACATCT GTTTCCACAC CAGCAGTGTT TGCATGTATC ACCAAGGACT TGTGGATGGA 
GGCCTTTCCG TCTTGGACGT CAATTTGCCG TCCAGGACAC ATCCTCACTG GTTAGATCCA 
ACTATCTACC AATCAGAGAC TTCCCCCGAG AGATCTTCAA CGCCCTCAGA GTGCATCGGC 
CTGGAGGCTC GGAGCATGGT GTCCGTGGCC GTCAAAGTAT CATCACCGAA CCCAGAGCAC 
AACATCAAGG AAATTCTTAT TGCTGTTGGA GTGAGAGGAG CCACCTTGCA GCATAAAATG 
GCCCCTCCTT GCACGGGTTG GTACGATCAG ATTGTTGACT TTCTGAATAT CACCAATGAG 
CCAGTGTTGG GATACGCCCC TCCCACCTCG GTCTCCACCC TACACTTACA CCTGTGGAGC 
TGTTCTCTTG ACTACAGACC CCTTTATCTG CCACTCAGAT CACTGTTGGT TGTAGAGACC 
TTTAGTATCT CCAGCAGTGT GTCTTTAGAT CACTCTTCCT CATCACTCAG GATCATATTG 
GATGAGGCTG CCGTGTTCCT GTCAGACAGG GCCAATGCCG TCTCTGTCAA TCTGGCACGT 
GACTATGTGC AAGTGGTGGA CATGGGAACT CTGGAGCTCA GGATCACAGC TGCCAAACCT 
GGAGCTGACG GAAAGCTGAA AGAGCCAAGG TTCGAACTGC GCTGCTCCAG CGACATTATT 
CACATCAGAA CCTGTTCGGA CTCCTGTGCT GCCCTCATGA ACCTTATTCA GTATGTAGCC 
AGCTATGGAG ACTTACTGCC CCCAGCAGAA CCAGAGCCCA AACACAGCAG TACCTCACAG 
AGGACCAAGG TTGAATTTCC AAGTCGGGTC ACACCTCAGA CGTCGTTTCT GGCTGATATT 
GAGCAGCAGA TGTTGCAGGA TTTGATGAGC GAAGCGATGG AGGAGACAGA AGGCCAGCAT 
GCATCAGGGC TGCAGCAGAA TGGCGCACGA GAAGAACACT GTCAAGATCA CGATCCACCC 
CGCTCAGACC TGTTCTTGTT CCCGGATGAG AGCGGAAATC TTAACCAAGA TTTAAGCCCC 
ACTTGTCCCG TCCTTCACTC TCCTCTCATC ACCCCTGTTC CCAATCTGGC CCAAGAGGCT 
GATGACTTTT GTATCCTGGA GACACCCGGC TTCAGACGAG AGGATCTTGA TCAGGAACCA 
GTGGTAAAGC GGCTTACTTC AGATCCTGTG GTCATTAAAG ATGACCATTT CAGCCAGCCC 
TTGTGTGGGA GTGACTCAAG CCACGGAGCC CTGAACTTTC CCATCCCAGA GGTGCGCTAC 
CTCATCAAGG AGATCTCTGT CATCTGGCAC CTCTACGGTG GGAAAGACTT TGGGAGCACA 
ACGTTAACAG CATCTCCTTC TAGAAGTTGG GGTTCTACAC CTCATAGCTC CCCCTCCCAG 
ACTCCGGTCC GACAATCCAA GAGTTCAAGC CGTTCTGGGG GAGGGAGAGG AAGGAACACT 
GATGTGTTGA TGGAGATACA GCTCAGCAAG GTGCGGTTTC AGCACGATGT GTACCCGCAG 
GCTCAGGGGG CTTCTGGGCC GACAGTGGAT CAGCCGGTGT CTCGACAGGT GTTTGTGGTC 
CAGGACTTGG AGATTCGAGA CAGACTAGCT GCCTCACAGA TGAATAAGTT CCTTTACTTG 
TATTCGAGCA AGGAAATGCC CAGAAAAGCT CATTCAAATA TGTTGACAGT TAAGGCTCTC 
CACATGTGTC CGGAGTCCAG CCAGGCTCCA CAGGAATGCT GCTTGCGTGT TTCACTCACG 
CCCCTTCGCC TCAATATTGA TCAGGATGCA CTGTTTTTCT TGAAGGACTT CTTTACCAGT 
CTTGCTACAG AGGTGGAGTT TTTCTCATCG CCATCACAAG AAGCCATCTG TGTTCCTATT 
AAAAAAGCAG CCACCCCAGA GATCTCTTGT AGCTTCTCCA AACACGCCAG CAGCAGCCAC 
GACCCAGCCC CAATCATCTC GCTGCCCACT CAGAGAGGTT TGAATCACAA TGGCTTCTCC 
AGCAGGGAGG AAATGGCTGA CAGTGAATCT TCTGCACCCT CGTTCACAGA CCAGCCCATA 
TTCTTTAGGG AGTTCAGATT TACCTCTGAG GTTCCAATTC GTCTGGATTA CCATGGAAAA 
CACGTGTCGA TGGAGCAGGG GACCTTTGCT GGAATAATTA TGGGGTTGAC ACAACTTAAC 
TGCTCAGAGT TGAAATTGAG GCGCTTGTGC TACAGACAAG GATTATTAGG TGTGGATAAG 
TTGTTTTCCT ATGCAATCAA CGAATGGCTT ACTGACATAA AGAAGAATCA GCTTCCGGGG 
CTGTTGGGTG GTGTGGGGCC TATTCACTCA CTGGTCCAGC TGGTTCAGGG ATTCAGGGAT 
TTGGTCTGGC TCCCCATCGA GCAGTACAGG AAAGACGGTC GGATTGTTCG TGGTTTTCAG 
AGGGGCACTG CCTCTTTCGG CACGTCGACA GCCATGGCCG CCCTGGAGCT CACCAACAGG 
ATGGTGCGGA CCATCCAGGC AGCAGCAGAG ACAGCCTACG ACATGGTGTC TCCAGTCCCT 
GATGAGAGAG ACACAAAGAG GGTAAAACGG TACATCCCTT ACAGACTGGC TCATCAGCCC 
GTCGACCTGA GAGAGGGCGT GGCCAAAGCT TACACAGTAG TAAAAGAGGG CATTACAGAC 
ACGGCACTGA CCATCTATGA CACAGCCACC AGGGAGCACG AACAGCGCGG GATGACAGGG 
GCAGTGGGCG GAGTTCTCCG CCAGCTGCCG CCAGCTGTGG TCAAGCCGCT CATCGTGGCC 
ACTGAGGCCA CCTCTAATGT CTTAGGTGGA ATGAGGAACC AGATTCACCC TGACGCAAGG 
CAGGAAGAGT CACAGAAGTG GAGGCAGGGC GAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029688500.1
CDS	364..6582
Translation	MPWPFSESIKKRACRYLLHRYLGNFLEEKLSLDQLSLDLYQGTGSLSQVPLDKWSLNELLETADAPFEVITGFIQTISLTVPWAALVQENCALEVKGLEMVFRPKPRVASGTEPMCWSSFMTSSMQLAKECLSQKLTDDLGESFQPFEGLEKFAETIETVLRRVKVTFLDTVLRIEHIPENSKTGIALEIRINKTVYCDETNEETSDVNVHQPTTFAHKNLQMEGITVFWDEFSEVSRAGCKSSPTPTETEPKLSPSWNPKIICEPHPQFTEPVYSAAPFEAVQVGSLGGKIKISLTLKNNLAMPGAKLDVDGHIDTLLILLSPRQVHLLLDLFGAFSGGGSQEWSKDRKSRPMQQEDEYRLHMELNRCLKKDTTVTGVDPELFESQTTRTVSSRDDVFFSMADMDMSHSLSSLPPLGEPPTVDLDLSLNSNYSASPGESPSANATALWDDYIDLPRHREKQVNESPIVLQDSQLHQNVLRQASHPSKPHGDESRPELLLNLKLSSLAISVLHIDPLPPPDAAHSPLGPMAAHFFRTVGPQHLAPAAFLESRNIFNQACPHDNLRFVAQGLKISYEHCKGSSLRTFNTDVSLNQMEFLECLFPSEGFSSDSQRGIQYTELLTFDMASPDVPLQTCLHLLYKQKERKGPQSGQFRLSTIPRKAEIQVELGPVRCELDISIVDRLNSLLQPQKLAATELMASHLYTSYNKHVSLHKAFTEVFLDDSHTPSNCHVSLTVNAPVLGLALRFPIPDLRSDQERGPWFKKSLQKEVLFLELEDLEVKTEFMAGNSPDQTKLELTFRELIGKFQQETNHPAVRFLRVSHTMDGDMTTSESVKFDWPRVVLKMNPTAVHSILERVQAEDDEGVEDHSLEEEEEEGAAHSLKDVCDFGKAEPSPFSSRRVMYENEEMVIPGDVTEMTEFQEKTMSNSRFIVELCLPNVQLSFPSKAFYETLHNRINNDLLLWEPTAPSPVETVENIPYGVGLSVASQLINTYSKDSFSQFRSSGPDDETSGSDEETMHYYIPASDPSLQSRKKKKPKAQSKTSQSLFSVIVSVNHGLIALQANAMKDKTVLKNKHGEFWLELKNGVLFSVMQHEGYKDQHYICFHTSSVCMYHQGLVDGGLSVLDVNLPSRTHPHWLDPTIYQSETSPERSSTPSECIGLEARSMVSVAVKVSSPNPEHNIKEILIAVGVRGATLQHKMAPPCTGWYDQIVDFLNITNEPVLGYAPPTSVSTLHLHLWSCSLDYRPLYLPLRSLLVVETFSISSSVSLDHSSSSLRIILDEAAVFLSDRANAVSVNLARDYVQVVDMGTLELRITAAKPGADGKLKEPRFELRCSSDIIHIRTCSDSCAALMNLIQYVASYGDLLPPAEPEPKHSSTSQRTKVEFPSRVTPQTSFLADIEQQMLQDLMSEAMEETEGQHASGLQQNGAREEHCQDHDPPRSDLFLFPDESGNLNQDLSPTCPVLHSPLITPVPNLAQEADDFCILETPGFRREDLDQEPVVKRLTSDPVVIKDDHFSQPLCGSDSSHGALNFPIPEVRYLIKEISVIWHLYGGKDFGSTTLTASPSRSWGSTPHSSPSQTPVRQSKSSSRSGGGRGRNTDVLMEIQLSKVRFQHDVYPQAQGASGPTVDQPVSRQVFVVQDLEIRDRLAASQMNKFLYLYSSKEMPRKAHSNMLTVKALHMCPESSQAPQECCLRVSLTPLRLNIDQDALFFLKDFFTSLATEVEFFSSPSQEAICVPIKKAATPEISCSFSKHASSSHDPAPIISLPTQRGLNHNGFSSREEMADSESSAPSFTDQPIFFREFRFTSEVPIRLDYHGKHVSMEQGTFAGIIMGLTQLNCSELKLRRLCYRQGLLGVDKLFSYAINEWLTDIKKNQLPGLLGGVGPIHSLVQLVQGFRDLVWLPIEQYRKDGRIVRGFQRGTASFGTSTAMAALELTNRMVRTIQAAAETAYDMVSPVPDERDTKRVKRYIPYRLAHQPVDLREGVAKAYTVVKEGITDTALTIYDTATREHEQRGMTGAVGGVLRQLPPAVVKPLIVATEATSNVLGGMRNQIHPDARQEESQKWRQGEE

Target ORF information:

RefSeq Version	XM_029832640.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes autophagy related 2B (atg2b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029832640.1

ORF Insert Sequence:

ATGCCTTGGC CTTTTTCTGA GTCCATCAAG AAGCGGGCCT GCAGATATCT CCTGCATAGG 
TACCTTGGCA ACTTTTTGGA AGAGAAACTA AGTTTGGATC AGCTCAGTTT AGATCTCTAT 
CAAGGTACTG GCTCACTTTC GCAGGTGCCG CTTGATAAAT GGTCGCTGAA TGAACTCCTG 
GAGACAGCAG ATGCACCGTT TGAAGTCATT ACTGGGTTTA TCCAGACCAT TTCTTTAACT 
GTCCCATGGG CAGCGCTTGT GCAGGAAAAT TGTGCCCTTG AGGTCAAAGG GTTAGAGATG 
GTCTTCAGAC CAAAACCAAG AGTAGCATCT GGCACTGAGC CCATGTGCTG GTCCAGTTTT 
ATGACAAGCA GCATGCAACT GGCCAAAGAA TGCCTAAGCC AGAAGCTCAC GGATGACCTC 
GGAGAGAGTT TTCAGCCTTT TGAAGGATTA GAGAAGTTTG CCGAAACAAT AGAAACAGTC 
CTGAGGAGAG TTAAAGTGAC ATTTTTGGAT ACAGTTCTCA GAATTGAACA CATTCCAGAA 
AATTCTAAAA CTGGAATCGC GCTGGAGATT CGAATTAACA AGACGGTTTA CTGTGATGAA 
ACTAACGAGG AGACTTCAGA CGTTAACGTG CATCAGCCGA CCACGTTTGC CCACAAAAAC 
CTGCAGATGG AAGGCATCAC TGTGTTCTGG GACGAGTTCT CCGAGGTGTC CCGCGCTGGC 
TGTAAATCTT CACCAACTCC CACGGAAACC GAACCAAAAC TGTCCCCGAG CTGGAATCCT 
AAAATAATCT GCGAGCCGCA TCCCCAGTTC ACAGAACCAG TATACTCTGC AGCACCATTT 
GAAGCTGTGC AGGTCGGGAG CCTTGGTGGG AAAATCAAGA TCTCCCTCAC TCTGAAGAAC 
AACCTAGCTA TGCCCGGAGC AAAGCTGGAT GTTGACGGTC ACATCGACAC ACTGTTGATT 
CTGCTGTCGC CGCGACAAGT TCATCTTCTT TTGGACCTAT TCGGAGCCTT TTCCGGTGGA 
GGTTCACAAG AATGGAGCAA GGACAGAAAG AGCAGACCCA TGCAACAGGA GGACGAGTAC 
CGGCTCCACA TGGAGCTGAA CCGGTGTCTA AAGAAAGACA CCACGGTCAC AGGAGTCGAC 
CCTGAATTGT TTGAAAGCCA AACCACAAGA ACCGTGTCGA GTCGAGACGA CGTGTTCTTT 
TCCATGGCCG ACATGGATAT GTCTCACAGC CTGTCGTCGC TGCCTCCTCT TGGTGAACCG 
CCCACAGTCG ACTTGGATTT ATCTCTTAAC AGCAACTACT CTGCTTCTCC TGGAGAATCT 
CCCTCTGCTA ATGCAACAGC TCTGTGGGAT GATTACATTG ATCTACCTCG ACACAGAGAG 
AAGCAGGTTA ATGAAAGTCC AATTGTATTG CAAGACTCTC AACTTCATCA AAATGTATTG 
AGACAAGCGT CCCACCCTTC CAAACCTCAT GGTGATGAGT CTCGACCAGA GCTGCTTTTG 
AATCTGAAGC TGAGCAGCCT GGCCATCTCG GTCCTCCACA TCGACCCGTT GCCACCACCG 
GATGCAGCTC ACAGTCCCCT CGGTCCTATG GCTGCACACT TCTTCCGCAC AGTAGGGCCC 
CAGCATCTTG CCCCAGCGGC TTTCCTTGAG TCTCGAAACA TTTTTAACCA GGCCTGCCCC 
CATGACAATC TCAGGTTTGT GGCCCAGGGG CTCAAGATCA GCTATGAGCA CTGTAAGGGA 
TCCAGTCTGC GGACCTTCAA CACCGACGTC TCTCTCAACC AGATGGAGTT TTTAGAGTGC 
CTGTTTCCAT CTGAGGGTTT CAGCAGCGAC TCCCAAAGAG GCATTCAGTA CACAGAGCTC 
CTGACATTTG ACATGGCGAG CCCTGATGTG CCTCTGCAGA CCTGCCTTCA CCTGCTGTAC 
AAACAGAAAG AGAGGAAAGG CCCTCAGAGC GGTCAGTTTC GACTCAGCAC CATTCCCAGG 
AAAGCAGAAA TCCAGGTGGA GCTGGGCCCG GTGCGATGCG AGCTGGACAT TAGTATTGTA 
GACCGTCTCA ACTCGTTGCT CCAACCCCAG AAGCTGGCCG CCACAGAGCT GATGGCGTCT 
CACCTGTACA CATCCTATAA TAAACACGTC AGCCTGCATA AAGCCTTTAC AGAGGTTTTT 
CTGGATGACA GTCACACTCC CAGCAACTGT CACGTGTCAT TGACTGTAAA CGCGCCGGTG 
CTGGGCCTCG CTCTCCGCTT CCCTATTCCT GACCTGCGCT CAGATCAGGA ACGGGGCCCC 
TGGTTTAAGA AGTCCCTGCA GAAGGAAGTC CTCTTTCTGG AACTGGAAGA TCTGGAAGTC 
AAAACAGAAT TCATGGCTGG CAACTCACCT GATCAAACTA AGTTGGAGCT CACATTCAGG 
GAGCTCATTG GAAAATTTCA ACAGGAGACT AACCACCCAG CCGTCCGCTT CCTCCGAGTG 
TCTCACACCA TGGACGGAGA CATGACAACG TCAGAAAGTG TTAAATTTGA CTGGCCGAGG 
GTCGTGCTGA AGATGAACCC CACTGCAGTG CACTCGATAC TTGAGCGGGT GCAGGCCGAA 
GATGATGAGG GGGTAGAGGA CCATTCACTC GAAGAGGAAG AGGAGGAGGG CGCTGCACAT 
TCACTGAAGG ACGTGTGTGA CTTTGGTAAA GCAGAGCCAT CTCCGTTTTC ATCGCGAAGG 
GTCATGTACG AAAATGAGGA GATGGTCATT CCGGGTGACG TCACTGAGAT GACGGAATTC 
CAGGAGAAGA CCATGAGCAA CTCAAGGTTC ATTGTTGAGT TGTGTCTCCC CAATGTGCAG 
TTATCCTTCC CCAGCAAAGC ATTTTATGAA ACACTGCACA ACAGGATAAA TAACGATTTG 
CTGCTTTGGG AGCCGACTGC TCCATCTCCC GTGGAGACGG TGGAAAACAT CCCTTATGGA 
GTTGGCCTCT CTGTCGCCAG TCAGTTGATC AACACTTACT CCAAAGACAG CTTTAGTCAG 
TTTCGCTCCT CTGGTCCTGA TGATGAGACC AGCGGGTCAG ATGAAGAAAC CATGCATTAT 
TACATTCCTG CTTCAGACCC AAGCCTCCAG TCTCGCAAGA AGAAAAAGCC CAAAGCCCAA 
AGCAAGACCT CCCAGAGCCT ATTTTCTGTC ATCGTCAGTG TCAATCACGG TCTGATCGCG 
CTACAAGCTA ATGCTATGAA GGACAAAACT GTATTGAAAA ACAAACATGG TGAATTCTGG 
CTGGAGCTAA AGAATGGCGT GTTGTTCAGT GTCATGCAGC ACGAGGGCTA CAAAGATCAA 
CATTACATCT GTTTCCACAC CAGCAGTGTT TGCATGTATC ACCAAGGACT TGTGGATGGA 
GGCCTTTCCG TCTTGGACGT CAATTTGCCG TCCAGGACAC ATCCTCACTG GTTAGATCCA 
ACTATCTACC AATCAGAGAC TTCCCCCGAG AGATCTTCAA CGCCCTCAGA GTGCATCGGC 
CTGGAGGCTC GGAGCATGGT GTCCGTGGCC GTCAAAGTAT CATCACCGAA CCCAGAGCAC 
AACATCAAGG AAATTCTTAT TGCTGTTGGA GTGAGAGGAG CCACCTTGCA GCATAAAATG 
GCCCCTCCTT GCACGGGTTG GTACGATCAG ATTGTTGACT TTCTGAATAT CACCAATGAG 
CCAGTGTTGG GATACGCCCC TCCCACCTCG GTCTCCACCC TACACTTACA CCTGTGGAGC 
TGTTCTCTTG ACTACAGACC CCTTTATCTG CCACTCAGAT CACTGTTGGT TGTAGAGACC 
TTTAGTATCT CCAGCAGTGT GTCTTTAGAT CACTCTTCCT CATCACTCAG GATCATATTG 
GATGAGGCTG CCGTGTTCCT GTCAGACAGG GCCAATGCCG TCTCTGTCAA TCTGGCACGT 
GACTATGTGC AAGTGGTGGA CATGGGAACT CTGGAGCTCA GGATCACAGC TGCCAAACCT 
GGAGCTGACG GAAAGCTGAA AGAGCCAAGG TTCGAACTGC GCTGCTCCAG CGACATTATT 
CACATCAGAA CCTGTTCGGA CTCCTGTGCT GCCCTCATGA ACCTTATTCA GTATGTAGCC 
AGCTATGGAG ACTTACTGCC CCCAGCAGAA CCAGAGCCCA AACACAGCAG TACCTCACAG 
AGGACCAAGG TTGAATTTCC AAGTCGGGTC ACACCTCAGA CGTCGTTTCT GGCTGATATT 
GAGCAGCAGA TGTTGCAGGA TTTGATGAGC GAAGCGATGG AGGAGACAGA AGGCCAGCAT 
GCATCAGGGC TGCAGCAGAA TGGCGCACGA GAAGAACACT GTCAAGATCA CGATCCACCC 
CGCTCAGACC TGTTCTTGTT CCCGGATGAG AGCGGAAATC TTAACCAAGA TTTAAGCCCC 
ACTTGTCCCG TCCTTCACTC TCCTCTCATC ACCCCTGTTC CCAATCTGGC CCAAGAGGCT 
GATGACTTTT GTATCCTGGA GACACCCGGC TTCAGACGAG AGGATCTTGA TCAGGAACCA 
GTGGTAAAGC GGCTTACTTC AGATCCTGTG GTCATTAAAG ATGACCATTT CAGCCAGCCC 
TTGTGTGGGA GTGACTCAAG CCACGGAGCC CTGAACTTTC CCATCCCAGA GGTGCGCTAC 
CTCATCAAGG AGATCTCTGT CATCTGGCAC CTCTACGGTG GGAAAGACTT TGGGAGCACA 
ACGTTAACAG CATCTCCTTC TAGAAGTTGG GGTTCTACAC CTCATAGCTC CCCCTCCCAG 
ACTCCGGTCC GACAATCCAA GAGTTCAAGC CGTTCTGGGG GAGGGAGAGG AAGGAACACT 
GATGTGTTGA TGGAGATACA GCTCAGCAAG GTGCGGTTTC AGCACGATGT GTACCCGCAG 
GCTCAGGGGG CTTCTGGGCC GACAGTGGAT CAGCCGGTGT CTCGACAGGT GTTTGTGGTC 
CAGGACTTGG AGATTCGAGA CAGACTAGCT GCCTCACAGA TGAATAAGTT CCTTTACTTG 
TATTCGAGCA AGGAAATGCC CAGAAAAGCT CATTCAAATA TGTTGACAGT TAAGGCTCTC 
CACATGTGTC CGGAGTCCAG CCAGGCTCCA CAGGAATGCT GCTTGCGTGT TTCACTCACG 
CCCCTTCGCC TCAATATTGA TCAGGATGCA CTGTTTTTCT TGAAGGACTT CTTTACCAGT 
CTTGCTACAG AGGTGGAGTT TTTCTCATCG CCATCACAAG AAGCCATCTG TGTTCCTATT 
AAAAAAGCAG CCACCCCAGA GATCTCTTGT AGCTTCTCCA AACACGCCAG CAGCAGCCAC 
GACCCAGCCC CAATCATCTC GCTGCCCACT CAGAGAGGTT TGAATCACAA TGGCTTCTCC 
AGCAGGGAGG AAATGGCTGA CAGTGAATCT TCTGCACCCT CGTTCACAGA CCAGCCCATA 
TTCTTTAGGG AGTTCAGATT TACCTCTGAG GTTCCAATTC GTCTGGATTA CCATGGAAAA 
CACGTGTCGA TGGAGCAGGG GACCTTTGCT GGAATAATTA TGGGGTTGAC ACAACTTAAC 
TGCTCAGAGT TGAAATTGAG GCGCTTGTGC TACAGACAAG GATTATTAGG TGTGGATAAG 
TTGTTTTCCT ATGCAATCAA CGAATGGCTT ACTGACATAA AGAAGAATCA GCTTCCGGGG 
CTGTTGGGTG GTGTGGGGCC TATTCACTCA CTGGTCCAGC TGGTTCAGGG ATTCAGGGAT 
TTGGTCTGGC TCCCCATCGA GCAGTACAGG AAAGACGGTC GGATTGTTCG TGGTTTTCAG 
AGGGGCACTG CCTCTTTCGG CACGTCGACA GCCATGGCCG CCCTGGAGCT CACCAACAGG 
ATGGTGCGGA CCATCCAGGC AGCAGCAGAG ACAGCCTACG ACATGGTGTC TCCAGTCCCT 
GATGAGAGAG ACACAAAGAG GGTAAAACGG TACATCCCTT ACAGACTGGC TCATCAGCCC 
GTCGACCTGA GAGAGGGCGT GGCCAAAGCT TACACAGTAG TAAAAGAGGG CATTACAGAC 
ACGGCACTGA CCATCTATGA CACAGCCACC AGGGAGCACG AACAGCGCGG GATGACAGGG 
GCAGTGGGCG GAGTTCTCCG CCAGCTGCCG CCAGCTGTGG TCAAGCCGCT CATCGTGGCC 
ACTGAGGCCA CCTCTAATGT CTTAGGTGGA ATGAGGAACC AGATTCACCC TGACGCAAGG 
CAGGAAGAGT CACAGAAGTG GAGGCAGGGC GAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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atg2b cDNA ORF clone, Takifugu rubripes(Fugu rubripes)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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