DDX4 cDNA ORF clone, Sus scrofa(pig)

Gene
Clones
gRNAs

Summary

Gene Symbol	DDX4
Entrez Gene ID	431672
Full Name	DEAD-box helicase 4
Synonyms	VASA
Gene Type	protein-coding
Organism	Sus scrofa(pig)
Genome	Chromosome: 16 Map Location: 16\|16

mRNA and Protein(s)

mRNA	Protein	Name
XM_021076578.1	XP_020932237.1	probable ATP-dependent RNA helicase DDX4 isoform X2
XM_021076580.1	XP_020932239.1	probable ATP-dependent RNA helicase DDX4 isoform X4
XM_021076577.1	XP_020932236.1	probable ATP-dependent RNA helicase DDX4 isoform X1
XM_021076581.1	XP_020932240.1	probable ATP-dependent RNA helicase DDX4 isoform X5
NM_001291682.1	NP_001278611.1	probable ATP-dependent RNA helicase DDX4
XM_021076579.1	XP_020932238.1	probable ATP-dependent RNA helicase DDX4 isoform X3

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Stage-specific expression of DDX4 and c-kit at different developmental stages of the porcine testis.
Lee R, Lee WY, Park HJ, Ha WT, Woo JS, Chung HJ, Lee JH, Hong K, Song H
Animal reproduction science19018-26(2018 Mar)

Cell-surface DEAD-box polypeptide 4-immunoreactive cells and gonocytes are two distinct populations in postnatal porcine testes.
Kakiuchi K, Tsuda A, Goto Y, Shimada T, Taniguchi K, Takagishi K, Kubota H
Biology of reproduction90(4)82(2014 Apr)

Characterization of pig vasa homolog gene and specific expression in germ cell lineage.
Lee GS, Kim HS, Lee SH, Kang MS, Kim DY, Lee CK, Kang SK, Lee BC, Hwang WS
Molecular reproduction and development72(3)320-8(2005 Nov)

Expression and intracellular localization of mouse Vasa-homologue protein during germ cell development.
Toyooka Y, Tsunekawa N, Takahashi Y, Matsui Y, Satoh M, Noce T
Mechanisms of development93(1-2)139-49(2000 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following DDX4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DDX4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSe00643	NM_001291682.1 Latest version!	Sus scrofa DEAD-box helicase 4 (DDX4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OSe00643	XM_021076579.1 Latest version!	Sus scrofa DEAD-box helicase 4 (DDX4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OSe240450	XM_021076577.1 Latest version!	Sus scrofa DEAD-box helicase 4 (DDX4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OSe240451	XM_021076578.1 Latest version!	Sus scrofa DEAD-box helicase 4 (DDX4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OSe240452	XM_021076580.1 Latest version!	Sus scrofa DEAD-box helicase 4 (DDX4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OSe240453	XM_021076581.1 Latest version!	Sus scrofa DEAD-box helicase 4 (DDX4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DDX4 ( NM_001291682.1 ) cDNA ORF clone, Sus scrofa(pig) -> NP_001278611.1 Sus scrofa DEAD-box helicase 4 (DDX4), mRNA.

Price & Availability↑

CloneID	OSe00643
Clone ID Related Accession (Same CDS sequence)	NM_001291682.1 , XM_021076579.1
Accession Version	NM_001291682.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2190bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571587200000
Organism	Sus scrofa(pig)
Product	probable ATP-dependent RNA helicase DDX4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB823012.1. ##Evidence-Data-START## Transcript exon combination :: AB823012.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3180022, SAMN01915391 [ECO:0000348] ##Evidence-Data-END##

ATGGGAGATG AAGATTGGGA AGCAGAAATA ATCAAACCTC ATATGTCTTC CTATGTTCCC 
GTATTTGAGA AGGATAGGTA TTCTTCTGGA GCAAATGGAG ACACTTATAA TAGGACTCCA 
GCTTCATCAT CAGAAATGGA TGATGGATCT TCTCGAAGAG ATCACTTCAT GAGAAGTGGC 
TTTGCCTCTG GGAGGAGTTT GGGAAACCGG GATCCTGGCG AATCTAATAA AAGAGAGAAT 
ACATCCACAA TGGGTGGTTT TGGTGTTGGA AAGAGTTTTG GAAACAGAGG TTTTTCAAAT 
AACAAGTTTG AAGAAGGTGA TAGCTCTGGT TTCTGGAGAG AGTCTAATAA TGACTGTGAA 
GATAATCAGA CACGGAACAG AGGTTTTTCC AAGAGAGGCG GCTTTCAAGA TGGAAATGAT 
TCAGAAGCCT CAGTGTCGTC CAGAAGAGGT GGAAGAGGTA GTTTCCGAGG TTGCCGTGGA 
CGATTTGGTC TAGGAAGTCC AAATAGCGAA TATGAACAAG ATGAGGGGAC CCATCGTACT 
GGTGGCTTTT TTGGTTCTAG AAGATCAGCA TTAAGTGGTG CAGGTAATGG TGACACTTAC 
CAAAGCAGAA GTGGTAGTGG AAGTGGACGA GGTGGTTACA AAGGTCTAAA TGAAGAAGTA 
ATTACAGGCT CTGGAAAGAA TTCTTGGAAG TCAGAAGCTG AAGGAGGAGA AAGTGGTGAC 
ATTCAAGGAC CAAAAGTAAC CTACATACCA CCTCCTCCCC CTGAGGATGA GGACTCCATC 
TTTGCACATT ATCAGACAGG CATAAACTTT GACAAATATG ATACTATTCT TGTAGAAGTA 
TCTGGACATG AGGCACCGCC AGCAATTCTG ACTTTTGAAG AAGCTAATCT CTGTCAGACA 
CTGAATAGCA ACATTGCTAA AGCTGGTTAT ACTAAGCTTA CTCCTGTACA GAAGTACAGT 
ATTCCTATTA TACTAGGAGG ACGAGATTTG ATGGCTTGCG CTCAGACAGG GTCTGGAAAG 
ACTGCAGCCT TTCTCTTGCC AATTTTGGCT CATATGATGC GTGATGGAAT AACTGCCAGT 
CGTTTTAAAG AGCTGCAGGA ACCAGAGTGT ATTATTGTAG CACCAACTCG AGAATTGATC 
AATCAGATCT ATTTGGAAGC CAGAAAATTT TCTTTTGGGA CTTGTGTAAG AGCTGTTGTT 
ATATATGGGG GAACCCAGTT GGGGCATTCA ATTCGACAAA TAGTACAAGG CTGTAATATA 
TTATGTGCTA CTCCTGGGAG ACTGATGGAT ATCATAGGTA AAGAAAAGAT TGGTCTCAGA 
CAAGTCAAAT ATTTAGTTTT GGATGAAGCT GATCGCATGC TGGATATGGG TTTTGGACCA 
GAAATGAAGA AGTTAATTTC CTGCCCAGGA ATGCCATCAA AGGAACAACG TCAAACACTT 
ATGTTTAGTG CAACTTTTCC AGAAGAAATT CAAAGGTTGG CTGGGGAGTT TTTAAAATCG 
AATTATTTGT TTGTTGCTGT TGGACAAGTG GGCGGAGCAT GTAGAGATGT TCAGCAGACC 
ATTCTCCAAG TTGGCCAGTA CTCAAAAAGA GAAAAACTTG TTGAAATTCT ACGAAACATA 
GGTGATGAAA GAACTATGGT CTTTGTAGAA ACTAAGAAAA AAGCAGATTT TATTGCCACT 
TTTCTTTGTC AAGAAAAAAT ATCAACAACC AGTATTCATG GTGATCGAGA ACAGAGAGAG 
AGAGAGCAAG CTCTTGGAGA TTTTCGCTGT GGGAAGTGTC CAGTTCTTGT TGCTACTTCA 
GTAGCTGCCC GAGGGCTGGA TATTGAAAAT GTTCAGCATG TTATCAATTT TGATCTTCCT 
TCCACCATCG ATGAATATGT TCATCGAATT GGGCGTACGG GTCGCTGTGG AAATACTGGC 
AGAGCTATTT CCTTTTTTGA TCCTGAATCA GATAGCCATT TAGCACAGCC ACTAGTGAAA 
GTACTATCAG ATGCTCAACA GGATGTTCCT GCGTGGTTAG AAGAAATTGC ATTTAGTACC 
TATGTTCCTG GCTTCAGTGG TAGTTCAAGA GGAAATGTGT TTGCATCAGT TGATACTAGA 
AAGAATTACC AGGGCAAGAG CTCTTTGAAC ACAGCAGGGT TTTCTTCTTC ACAAGCTCCC 
AATCCAGTAG ATGATGAGTC ATGGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001278611.1
CDS	15..2204
Translation	MGDEDWEAEIIKPHMSSYVPVFEKDRYSSGANGDTYNRTPASSSEMDDGSSRRDHFMRSGFASGRSLGNRDPGESNKRENTSTMGGFGVGKSFGNRGFSNNKFEEGDSSGFWRESNNDCEDNQTRNRGFSKRGGFQDGNDSEASVSSRRGGRGSFRGCRGRFGLGSPNSEYEQDEGTHRTGGFFGSRRSALSGAGNGDTYQSRSGSGSGRGGYKGLNEEVITGSGKNSWKSEAEGGESGDIQGPKVTYIPPPPPEDEDSIFAHYQTGINFDKYDTILVEVSGHEAPPAILTFEEANLCQTLNSNIAKAGYTKLTPVQKYSIPIILGGRDLMACAQTGSGKTAAFLLPILAHMMRDGITASRFKELQEPECIIVAPTRELINQIYLEARKFSFGTCVRAVVIYGGTQLGHSIRQIVQGCNILCATPGRLMDIIGKEKIGLRQVKYLVLDEADRMLDMGFGPEMKKLISCPGMPSKEQRQTLMFSATFPEEIQRLAGEFLKSNYLFVAVGQVGGACRDVQQTILQVGQYSKREKLVEILRNIGDERTMVFVETKKKADFIATFLCQEKISTTSIHGDREQREREQALGDFRCGKCPVLVATSVAARGLDIENVQHVINFDLPSTIDEYVHRIGRTGRCGNTGRAISFFDPESDSHLAQPLVKVLSDAQQDVPAWLEEIAFSTYVPGFSGSSRGNVFASVDTRKNYQGKSSLNTAGFSSSQAPNPVDDESWD

Target ORF information:

RefSeq Version	NM_001291682.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa DEAD-box helicase 4 (DDX4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001291682.1

ORF Insert Sequence:

ATGGGAGATG AAGATTGGGA AGCAGAAATA ATCAAACCTC ATATGTCTTC CTATGTTCCC 
GTATTTGAGA AGGATAGGTA TTCTTCTGGA GCAAATGGAG ACACTTATAA TAGGACTCCA 
GCTTCATCAT CAGAAATGGA TGATGGATCT TCTCGAAGAG ATCACTTCAT GAGAAGTGGC 
TTTGCCTCTG GGAGGAGTTT GGGAAACCGG GATCCTGGCG AATCTAATAA AAGAGAGAAT 
ACATCCACAA TGGGTGGTTT TGGTGTTGGA AAGAGTTTTG GAAACAGAGG TTTTTCAAAT 
AACAAGTTTG AAGAAGGTGA TAGCTCTGGT TTCTGGAGAG AGTCTAATAA TGACTGTGAA 
GATAATCAGA CACGGAACAG AGGTTTTTCC AAGAGAGGCG GCTTTCAAGA TGGAAATGAT 
TCAGAAGCCT CAGTGTCGTC CAGAAGAGGT GGAAGAGGTA GTTTCCGAGG TTGCCGTGGA 
CGATTTGGTC TAGGAAGTCC AAATAGCGAA TATGAACAAG ATGAGGGGAC CCATCGTACT 
GGTGGCTTTT TTGGTTCTAG AAGATCAGCA TTAAGTGGTG CAGGTAATGG TGACACTTAC 
CAAAGCAGAA GTGGTAGTGG AAGTGGACGA GGTGGTTACA AAGGTCTAAA TGAAGAAGTA 
ATTACAGGCT CTGGAAAGAA TTCTTGGAAG TCAGAAGCTG AAGGAGGAGA AAGTGGTGAC 
ATTCAAGGAC CAAAAGTAAC CTACATACCA CCTCCTCCCC CTGAGGATGA GGACTCCATC 
TTTGCACATT ATCAGACAGG CATAAACTTT GACAAATATG ATACTATTCT TGTAGAAGTA 
TCTGGACATG AGGCACCGCC AGCAATTCTG ACTTTTGAAG AAGCTAATCT CTGTCAGACA 
CTGAATAGCA ACATTGCTAA AGCTGGTTAT ACTAAGCTTA CTCCTGTACA GAAGTACAGT 
ATTCCTATTA TACTAGGAGG ACGAGATTTG ATGGCTTGCG CTCAGACAGG GTCTGGAAAG 
ACTGCAGCCT TTCTCTTGCC AATTTTGGCT CATATGATGC GTGATGGAAT AACTGCCAGT 
CGTTTTAAAG AGCTGCAGGA ACCAGAGTGT ATTATTGTAG CACCAACTCG AGAATTGATC 
AATCAGATCT ATTTGGAAGC CAGAAAATTT TCTTTTGGGA CTTGTGTAAG AGCTGTTGTT 
ATATATGGGG GAACCCAGTT GGGGCATTCA ATTCGACAAA TAGTACAAGG CTGTAATATA 
TTATGTGCTA CTCCTGGGAG ACTGATGGAT ATCATAGGTA AAGAAAAGAT TGGTCTCAGA 
CAAGTCAAAT ATTTAGTTTT GGATGAAGCT GATCGCATGC TGGATATGGG TTTTGGACCA 
GAAATGAAGA AGTTAATTTC CTGCCCAGGA ATGCCATCAA AGGAACAACG TCAAACACTT 
ATGTTTAGTG CAACTTTTCC AGAAGAAATT CAAAGGTTGG CTGGGGAGTT TTTAAAATCG 
AATTATTTGT TTGTTGCTGT TGGACAAGTG GGCGGAGCAT GTAGAGATGT TCAGCAGACC 
ATTCTCCAAG TTGGCCAGTA CTCAAAAAGA GAAAAACTTG TTGAAATTCT ACGAAACATA 
GGTGATGAAA GAACTATGGT CTTTGTAGAA ACTAAGAAAA AAGCAGATTT TATTGCCACT 
TTTCTTTGTC AAGAAAAAAT ATCAACAACC AGTATTCATG GTGATCGAGA ACAGAGAGAG 
AGAGAGCAAG CTCTTGGAGA TTTTCGCTGT GGGAAGTGTC CAGTTCTTGT TGCTACTTCA 
GTAGCTGCCC GAGGGCTGGA TATTGAAAAT GTTCAGCATG TTATCAATTT TGATCTTCCT 
TCCACCATCG ATGAATATGT TCATCGAATT GGGCGTACGG GTCGCTGTGG AAATACTGGC 
AGAGCTATTT CCTTTTTTGA TCCTGAATCA GATAGCCATT TAGCACAGCC ACTAGTGAAA 
GTACTATCAG ATGCTCAACA GGATGTTCCT GCGTGGTTAG AAGAAATTGC ATTTAGTACC 
TATGTTCCTG GCTTCAGTGG TAGTTCAAGA GGAAATGTGT TTGCATCAGT TGATACTAGA 
AAGAATTACC AGGGCAAGAG CTCTTTGAAC ACAGCAGGGT TTTCTTCTTC ACAAGCTCCC 
AATCCAGTAG ATGATGAGTC ATGGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX4 ( XM_021076579.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020932238.1 Sus scrofa DEAD-box helicase 4 (DDX4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OSe00643
Clone ID Related Accession (Same CDS sequence)	NM_001291682.1 , XM_021076579.1
Accession Version	XM_021076579.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2190bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	probable ATP-dependent RNA helicase DDX4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010458.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGATG AAGATTGGGA AGCAGAAATA ATCAAACCTC ATATGTCTTC CTATGTTCCC 
GTATTTGAGA AGGATAGGTA TTCTTCTGGA GCAAATGGAG ACACTTATAA TAGGACTCCA 
GCTTCATCAT CAGAAATGGA TGATGGATCT TCTCGAAGAG ATCACTTCAT GAGAAGTGGC 
TTTGCCTCTG GGAGGAGTTT GGGAAACCGG GATCCTGGCG AATCTAATAA AAGAGAGAAT 
ACATCCACAA TGGGTGGTTT TGGTGTTGGA AAGAGTTTTG GAAACAGAGG TTTTTCAAAT 
AACAAGTTTG AAGAAGGTGA TAGCTCTGGT TTCTGGAGAG AGTCTAATAA TGACTGTGAA 
GATAATCAGA CACGGAACAG AGGTTTTTCC AAGAGAGGCG GCTTTCAAGA TGGAAATGAT 
TCAGAAGCCT CAGTGTCGTC CAGAAGAGGT GGAAGAGGTA GTTTCCGAGG TTGCCGTGGA 
CGATTTGGTC TAGGAAGTCC AAATAGCGAA TATGAACAAG ATGAGGGGAC CCATCGTACT 
GGTGGCTTTT TTGGTTCTAG AAGATCAGCA TTAAGTGGTG CAGGTAATGG TGACACTTAC 
CAAAGCAGAA GTGGTAGTGG AAGTGGACGA GGTGGTTACA AAGGTCTAAA TGAAGAAGTA 
ATTACAGGCT CTGGAAAGAA TTCTTGGAAG TCAGAAGCTG AAGGAGGAGA AAGTGGTGAC 
ATTCAAGGAC CAAAAGTAAC CTACATACCA CCTCCTCCCC CTGAGGATGA GGACTCCATC 
TTTGCACATT ATCAGACAGG CATAAACTTT GACAAATATG ATACTATTCT TGTAGAAGTA 
TCTGGACATG AGGCACCGCC AGCAATTCTG ACTTTTGAAG AAGCTAATCT CTGTCAGACA 
CTGAATAGCA ACATTGCTAA AGCTGGTTAT ACTAAGCTTA CTCCTGTACA GAAGTACAGT 
ATTCCTATTA TACTAGGAGG ACGAGATTTG ATGGCTTGCG CTCAGACAGG GTCTGGAAAG 
ACTGCAGCCT TTCTCTTGCC AATTTTGGCT CATATGATGC GTGATGGAAT AACTGCCAGT 
CGTTTTAAAG AGCTGCAGGA ACCAGAGTGT ATTATTGTAG CACCAACTCG AGAATTGATC 
AATCAGATCT ATTTGGAAGC CAGAAAATTT TCTTTTGGGA CTTGTGTAAG AGCTGTTGTT 
ATATATGGGG GAACCCAGTT GGGGCATTCA ATTCGACAAA TAGTACAAGG CTGTAATATA 
TTATGTGCTA CTCCTGGGAG ACTGATGGAT ATCATAGGTA AAGAAAAGAT TGGTCTCAGA 
CAAGTCAAAT ATTTAGTTTT GGATGAAGCT GATCGCATGC TGGATATGGG TTTTGGACCA 
GAAATGAAGA AGTTAATTTC CTGCCCAGGA ATGCCATCAA AGGAACAACG TCAAACACTT 
ATGTTTAGTG CAACTTTTCC AGAAGAAATT CAAAGGTTGG CTGGGGAGTT TTTAAAATCG 
AATTATTTGT TTGTTGCTGT TGGACAAGTG GGCGGAGCAT GTAGAGATGT TCAGCAGACC 
ATTCTCCAAG TTGGCCAGTA CTCAAAAAGA GAAAAACTTG TTGAAATTCT ACGAAACATA 
GGTGATGAAA GAACTATGGT CTTTGTAGAA ACTAAGAAAA AAGCAGATTT TATTGCCACT 
TTTCTTTGTC AAGAAAAAAT ATCAACAACC AGTATTCATG GTGATCGAGA ACAGAGAGAG 
AGAGAGCAAG CTCTTGGAGA TTTTCGCTGT GGGAAGTGTC CAGTTCTTGT TGCTACTTCA 
GTAGCTGCCC GAGGGCTGGA TATTGAAAAT GTTCAGCATG TTATCAATTT TGATCTTCCT 
TCCACCATCG ATGAATATGT TCATCGAATT GGGCGTACGG GTCGCTGTGG AAATACTGGC 
AGAGCTATTT CCTTTTTTGA TCCTGAATCA GATAGCCATT TAGCACAGCC ACTAGTGAAA 
GTACTATCAG ATGCTCAACA GGATGTTCCT GCGTGGTTAG AAGAAATTGC ATTTAGTACC 
TATGTTCCTG GCTTCAGTGG TAGTTCAAGA GGAAATGTGT TTGCATCAGT TGATACTAGA 
AAGAATTACC AGGGCAAGAG CTCTTTGAAC ACAGCAGGGT TTTCTTCTTC ACAAGCTCCC 
AATCCAGTAG ATGATGAGTC ATGGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020932238.1
CDS	1060..3249
Translation	MGDEDWEAEIIKPHMSSYVPVFEKDRYSSGANGDTYNRTPASSSEMDDGSSRRDHFMRSGFASGRSLGNRDPGESNKRENTSTMGGFGVGKSFGNRGFSNNKFEEGDSSGFWRESNNDCEDNQTRNRGFSKRGGFQDGNDSEASVSSRRGGRGSFRGCRGRFGLGSPNSEYEQDEGTHRTGGFFGSRRSALSGAGNGDTYQSRSGSGSGRGGYKGLNEEVITGSGKNSWKSEAEGGESGDIQGPKVTYIPPPPPEDEDSIFAHYQTGINFDKYDTILVEVSGHEAPPAILTFEEANLCQTLNSNIAKAGYTKLTPVQKYSIPIILGGRDLMACAQTGSGKTAAFLLPILAHMMRDGITASRFKELQEPECIIVAPTRELINQIYLEARKFSFGTCVRAVVIYGGTQLGHSIRQIVQGCNILCATPGRLMDIIGKEKIGLRQVKYLVLDEADRMLDMGFGPEMKKLISCPGMPSKEQRQTLMFSATFPEEIQRLAGEFLKSNYLFVAVGQVGGACRDVQQTILQVGQYSKREKLVEILRNIGDERTMVFVETKKKADFIATFLCQEKISTTSIHGDREQREREQALGDFRCGKCPVLVATSVAARGLDIENVQHVINFDLPSTIDEYVHRIGRTGRCGNTGRAISFFDPESDSHLAQPLVKVLSDAQQDVPAWLEEIAFSTYVPGFSGSSRGNVFASVDTRKNYQGKSSLNTAGFSSSQAPNPVDDESWD

Target ORF information:

RefSeq Version	XM_021076579.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa DEAD-box helicase 4 (DDX4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021076579.1

ORF Insert Sequence:

ATGGGAGATG AAGATTGGGA AGCAGAAATA ATCAAACCTC ATATGTCTTC CTATGTTCCC 
GTATTTGAGA AGGATAGGTA TTCTTCTGGA GCAAATGGAG ACACTTATAA TAGGACTCCA 
GCTTCATCAT CAGAAATGGA TGATGGATCT TCTCGAAGAG ATCACTTCAT GAGAAGTGGC 
TTTGCCTCTG GGAGGAGTTT GGGAAACCGG GATCCTGGCG AATCTAATAA AAGAGAGAAT 
ACATCCACAA TGGGTGGTTT TGGTGTTGGA AAGAGTTTTG GAAACAGAGG TTTTTCAAAT 
AACAAGTTTG AAGAAGGTGA TAGCTCTGGT TTCTGGAGAG AGTCTAATAA TGACTGTGAA 
GATAATCAGA CACGGAACAG AGGTTTTTCC AAGAGAGGCG GCTTTCAAGA TGGAAATGAT 
TCAGAAGCCT CAGTGTCGTC CAGAAGAGGT GGAAGAGGTA GTTTCCGAGG TTGCCGTGGA 
CGATTTGGTC TAGGAAGTCC AAATAGCGAA TATGAACAAG ATGAGGGGAC CCATCGTACT 
GGTGGCTTTT TTGGTTCTAG AAGATCAGCA TTAAGTGGTG CAGGTAATGG TGACACTTAC 
CAAAGCAGAA GTGGTAGTGG AAGTGGACGA GGTGGTTACA AAGGTCTAAA TGAAGAAGTA 
ATTACAGGCT CTGGAAAGAA TTCTTGGAAG TCAGAAGCTG AAGGAGGAGA AAGTGGTGAC 
ATTCAAGGAC CAAAAGTAAC CTACATACCA CCTCCTCCCC CTGAGGATGA GGACTCCATC 
TTTGCACATT ATCAGACAGG CATAAACTTT GACAAATATG ATACTATTCT TGTAGAAGTA 
TCTGGACATG AGGCACCGCC AGCAATTCTG ACTTTTGAAG AAGCTAATCT CTGTCAGACA 
CTGAATAGCA ACATTGCTAA AGCTGGTTAT ACTAAGCTTA CTCCTGTACA GAAGTACAGT 
ATTCCTATTA TACTAGGAGG ACGAGATTTG ATGGCTTGCG CTCAGACAGG GTCTGGAAAG 
ACTGCAGCCT TTCTCTTGCC AATTTTGGCT CATATGATGC GTGATGGAAT AACTGCCAGT 
CGTTTTAAAG AGCTGCAGGA ACCAGAGTGT ATTATTGTAG CACCAACTCG AGAATTGATC 
AATCAGATCT ATTTGGAAGC CAGAAAATTT TCTTTTGGGA CTTGTGTAAG AGCTGTTGTT 
ATATATGGGG GAACCCAGTT GGGGCATTCA ATTCGACAAA TAGTACAAGG CTGTAATATA 
TTATGTGCTA CTCCTGGGAG ACTGATGGAT ATCATAGGTA AAGAAAAGAT TGGTCTCAGA 
CAAGTCAAAT ATTTAGTTTT GGATGAAGCT GATCGCATGC TGGATATGGG TTTTGGACCA 
GAAATGAAGA AGTTAATTTC CTGCCCAGGA ATGCCATCAA AGGAACAACG TCAAACACTT 
ATGTTTAGTG CAACTTTTCC AGAAGAAATT CAAAGGTTGG CTGGGGAGTT TTTAAAATCG 
AATTATTTGT TTGTTGCTGT TGGACAAGTG GGCGGAGCAT GTAGAGATGT TCAGCAGACC 
ATTCTCCAAG TTGGCCAGTA CTCAAAAAGA GAAAAACTTG TTGAAATTCT ACGAAACATA 
GGTGATGAAA GAACTATGGT CTTTGTAGAA ACTAAGAAAA AAGCAGATTT TATTGCCACT 
TTTCTTTGTC AAGAAAAAAT ATCAACAACC AGTATTCATG GTGATCGAGA ACAGAGAGAG 
AGAGAGCAAG CTCTTGGAGA TTTTCGCTGT GGGAAGTGTC CAGTTCTTGT TGCTACTTCA 
GTAGCTGCCC GAGGGCTGGA TATTGAAAAT GTTCAGCATG TTATCAATTT TGATCTTCCT 
TCCACCATCG ATGAATATGT TCATCGAATT GGGCGTACGG GTCGCTGTGG AAATACTGGC 
AGAGCTATTT CCTTTTTTGA TCCTGAATCA GATAGCCATT TAGCACAGCC ACTAGTGAAA 
GTACTATCAG ATGCTCAACA GGATGTTCCT GCGTGGTTAG AAGAAATTGC ATTTAGTACC 
TATGTTCCTG GCTTCAGTGG TAGTTCAAGA GGAAATGTGT TTGCATCAGT TGATACTAGA 
AAGAATTACC AGGGCAAGAG CTCTTTGAAC ACAGCAGGGT TTTCTTCTTC ACAAGCTCCC 
AATCCAGTAG ATGATGAGTC ATGGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX4 ( XM_021076577.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020932236.1 Sus scrofa DEAD-box helicase 4 (DDX4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSe240450
Clone ID Related Accession (Same CDS sequence)	XM_021076577.1
Accession Version	XM_021076577.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	probable ATP-dependent RNA helicase DDX4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010458.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAACG GATTAGAACT GGAAGCCACC ATGGGAGATG AAGATTGGGA AGCAGAAATA 
ATCAAACCTC ATATGTCTTC CTATGTTCCC GTATTTGAGA AGGATAGGTA TTCTTCTGGA 
GCAAATGGAG ACACTTATAA TAGGACTCCA GCTTCATCAT CAGAAATGGA TGATGGATCT 
TCTCGAAGAG ATCACTTCAT GAGAAGTGGC TTTGCCTCTG GGAGGAGTTT GGGAAACCGG 
GATCCTGGCG AATCTAATAA AAGAGAGAAT ACATCCACAA TGGGTGGTTT TGGTGTTGGA 
AAGAGTTTTG GAAACAGAGG TTTTTCAAAT AACAAGTTTG AAGAAGGTGA TAGCTCTGGT 
TTCTGGAGAG AGTCTAATAA TGACTGTGAA GATAATCAGA CACGGAACAG AGGTTTTTCC 
AAGAGAGGCG TGACCAACCT CACTAAACTT AATGGCTTTC AAGATGGAAA TGATTCAGAA 
GCCTCAGTGT CGTCCAGAAG AGGTGGAAGA GGTAGTTTCC GAGGTTGCCG TGGACGATTT 
GGTCTAGGAA GTCCAAATAG CGAATATGAA CAAGATGAGG GGACCCATCG TACTGGTGGC 
TTTTTTGGTT CTAGAAGATC AGCATTAAGT GGTGCAGGTA ATGGTGACAC TTACCAAAGC 
AGAAGTGGTA GTGGAAGTGG ACGAGGTGGT TACAAAGGTC TAAATGAAGA AGTAATTACA 
GGCTCTGGAA AGAATTCTTG GAAGTCAGAA GCTGAAGGAG GAGAAAGTGG TGACATTCAA 
GGACCAAAAG TAACCTACAT ACCACCTCCT CCCCCTGAGG ATGAGGACTC CATCTTTGCA 
CATTATCAGA CAGGCATAAA CTTTGACAAA TATGATACTA TTCTTGTAGA AGTATCTGGA 
CATGAGGCAC CGCCAGCAAT TCTGACTTTT GAAGAAGCTA ATCTCTGTCA GACACTGAAT 
AGCAACATTG CTAAAGCTGG TTATACTAAG CTTACTCCTG TACAGAAGTA CAGTATTCCT 
ATTATACTAG GAGGACGAGA TTTGATGGCT TGCGCTCAGA CAGGGTCTGG AAAGACTGCA 
GCCTTTCTCT TGCCAATTTT GGCTCATATG ATGCGTGATG GAATAACTGC CAGTCGTTTT 
AAAGAGCTGC AGGAACCAGA GTGTATTATT GTAGCACCAA CTCGAGAATT GATCAATCAG 
ATCTATTTGG AAGCCAGAAA ATTTTCTTTT GGGACTTGTG TAAGAGCTGT TGTTATATAT 
GGGGGAACCC AGTTGGGGCA TTCAATTCGA CAAATAGTAC AAGGCTGTAA TATATTATGT 
GCTACTCCTG GGAGACTGAT GGATATCATA GGTAAAGAAA AGATTGGTCT CAGACAAGTC 
AAATATTTAG TTTTGGATGA AGCTGATCGC ATGCTGGATA TGGGTTTTGG ACCAGAAATG 
AAGAAGTTAA TTTCCTGCCC AGGAATGCCA TCAAAGGAAC AACGTCAAAC ACTTATGTTT 
AGTGCAACTT TTCCAGAAGA AATTCAAAGG TTGGCTGGGG AGTTTTTAAA ATCGAATTAT 
TTGTTTGTTG CTGTTGGACA AGTGGGCGGA GCATGTAGAG ATGTTCAGCA GACCATTCTC 
CAAGTTGGCC AGTACTCAAA AAGAGAAAAA CTTGTTGAAA TTCTACGAAA CATAGGTGAT 
GAAAGAACTA TGGTCTTTGT AGAAACTAAG AAAAAAGCAG ATTTTATTGC CACTTTTCTT 
TGTCAAGAAA AAATATCAAC AACCAGTATT CATGGTGATC GAGAACAGAG AGAGAGAGAG 
CAAGCTCTTG GAGATTTTCG CTGTGGGAAG TGTCCAGTTC TTGTTGCTAC TTCAGTAGCT 
GCCCGAGGGC TGGATATTGA AAATGTTCAG CATGTTATCA ATTTTGATCT TCCTTCCACC 
ATCGATGAAT ATGTTCATCG AATTGGGCGT ACGGGTCGCT GTGGAAATAC TGGCAGAGCT 
ATTTCCTTTT TTGATCCTGA ATCAGATAGC CATTTAGCAC AGCCACTAGT GAAAGTACTA 
TCAGATGCTC AACAGGATGT TCCTGCGTGG TTAGAAGAAA TTGCATTTAG TACCTATGTT 
CCTGGCTTCA GTGGTAGTTC AAGAGGAAAT GTGTTTGCAT CAGTTGATAC TAGAAAGAAT 
TACCAGGGCA AGAGCTCTTT GAACACAGCA GGGTTTTCTT CTTCACAAGC TCCCAATCCA 
GTAGATGATG AGTCATGGGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020932236.1
CDS	4..2247
Translation	MKNGLELEATMGDEDWEAEIIKPHMSSYVPVFEKDRYSSGANGDTYNRTPASSSEMDDGSSRRDHFMRSGFASGRSLGNRDPGESNKRENTSTMGGFGVGKSFGNRGFSNNKFEEGDSSGFWRESNNDCEDNQTRNRGFSKRGVTNLTKLNGFQDGNDSEASVSSRRGGRGSFRGCRGRFGLGSPNSEYEQDEGTHRTGGFFGSRRSALSGAGNGDTYQSRSGSGSGRGGYKGLNEEVITGSGKNSWKSEAEGGESGDIQGPKVTYIPPPPPEDEDSIFAHYQTGINFDKYDTILVEVSGHEAPPAILTFEEANLCQTLNSNIAKAGYTKLTPVQKYSIPIILGGRDLMACAQTGSGKTAAFLLPILAHMMRDGITASRFKELQEPECIIVAPTRELINQIYLEARKFSFGTCVRAVVIYGGTQLGHSIRQIVQGCNILCATPGRLMDIIGKEKIGLRQVKYLVLDEADRMLDMGFGPEMKKLISCPGMPSKEQRQTLMFSATFPEEIQRLAGEFLKSNYLFVAVGQVGGACRDVQQTILQVGQYSKREKLVEILRNIGDERTMVFVETKKKADFIATFLCQEKISTTSIHGDREQREREQALGDFRCGKCPVLVATSVAARGLDIENVQHVINFDLPSTIDEYVHRIGRTGRCGNTGRAISFFDPESDSHLAQPLVKVLSDAQQDVPAWLEEIAFSTYVPGFSGSSRGNVFASVDTRKNYQGKSSLNTAGFSSSQAPNPVDDESWD

Target ORF information:

RefSeq Version	XM_021076577.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa DEAD-box helicase 4 (DDX4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021076577.1

ORF Insert Sequence:

ATGAAGAACG GATTAGAACT GGAAGCCACC ATGGGAGATG AAGATTGGGA AGCAGAAATA 
ATCAAACCTC ATATGTCTTC CTATGTTCCC GTATTTGAGA AGGATAGGTA TTCTTCTGGA 
GCAAATGGAG ACACTTATAA TAGGACTCCA GCTTCATCAT CAGAAATGGA TGATGGATCT 
TCTCGAAGAG ATCACTTCAT GAGAAGTGGC TTTGCCTCTG GGAGGAGTTT GGGAAACCGG 
GATCCTGGCG AATCTAATAA AAGAGAGAAT ACATCCACAA TGGGTGGTTT TGGTGTTGGA 
AAGAGTTTTG GAAACAGAGG TTTTTCAAAT AACAAGTTTG AAGAAGGTGA TAGCTCTGGT 
TTCTGGAGAG AGTCTAATAA TGACTGTGAA GATAATCAGA CACGGAACAG AGGTTTTTCC 
AAGAGAGGCG TGACCAACCT CACTAAACTT AATGGCTTTC AAGATGGAAA TGATTCAGAA 
GCCTCAGTGT CGTCCAGAAG AGGTGGAAGA GGTAGTTTCC GAGGTTGCCG TGGACGATTT 
GGTCTAGGAA GTCCAAATAG CGAATATGAA CAAGATGAGG GGACCCATCG TACTGGTGGC 
TTTTTTGGTT CTAGAAGATC AGCATTAAGT GGTGCAGGTA ATGGTGACAC TTACCAAAGC 
AGAAGTGGTA GTGGAAGTGG ACGAGGTGGT TACAAAGGTC TAAATGAAGA AGTAATTACA 
GGCTCTGGAA AGAATTCTTG GAAGTCAGAA GCTGAAGGAG GAGAAAGTGG TGACATTCAA 
GGACCAAAAG TAACCTACAT ACCACCTCCT CCCCCTGAGG ATGAGGACTC CATCTTTGCA 
CATTATCAGA CAGGCATAAA CTTTGACAAA TATGATACTA TTCTTGTAGA AGTATCTGGA 
CATGAGGCAC CGCCAGCAAT TCTGACTTTT GAAGAAGCTA ATCTCTGTCA GACACTGAAT 
AGCAACATTG CTAAAGCTGG TTATACTAAG CTTACTCCTG TACAGAAGTA CAGTATTCCT 
ATTATACTAG GAGGACGAGA TTTGATGGCT TGCGCTCAGA CAGGGTCTGG AAAGACTGCA 
GCCTTTCTCT TGCCAATTTT GGCTCATATG ATGCGTGATG GAATAACTGC CAGTCGTTTT 
AAAGAGCTGC AGGAACCAGA GTGTATTATT GTAGCACCAA CTCGAGAATT GATCAATCAG 
ATCTATTTGG AAGCCAGAAA ATTTTCTTTT GGGACTTGTG TAAGAGCTGT TGTTATATAT 
GGGGGAACCC AGTTGGGGCA TTCAATTCGA CAAATAGTAC AAGGCTGTAA TATATTATGT 
GCTACTCCTG GGAGACTGAT GGATATCATA GGTAAAGAAA AGATTGGTCT CAGACAAGTC 
AAATATTTAG TTTTGGATGA AGCTGATCGC ATGCTGGATA TGGGTTTTGG ACCAGAAATG 
AAGAAGTTAA TTTCCTGCCC AGGAATGCCA TCAAAGGAAC AACGTCAAAC ACTTATGTTT 
AGTGCAACTT TTCCAGAAGA AATTCAAAGG TTGGCTGGGG AGTTTTTAAA ATCGAATTAT 
TTGTTTGTTG CTGTTGGACA AGTGGGCGGA GCATGTAGAG ATGTTCAGCA GACCATTCTC 
CAAGTTGGCC AGTACTCAAA AAGAGAAAAA CTTGTTGAAA TTCTACGAAA CATAGGTGAT 
GAAAGAACTA TGGTCTTTGT AGAAACTAAG AAAAAAGCAG ATTTTATTGC CACTTTTCTT 
TGTCAAGAAA AAATATCAAC AACCAGTATT CATGGTGATC GAGAACAGAG AGAGAGAGAG 
CAAGCTCTTG GAGATTTTCG CTGTGGGAAG TGTCCAGTTC TTGTTGCTAC TTCAGTAGCT 
GCCCGAGGGC TGGATATTGA AAATGTTCAG CATGTTATCA ATTTTGATCT TCCTTCCACC 
ATCGATGAAT ATGTTCATCG AATTGGGCGT ACGGGTCGCT GTGGAAATAC TGGCAGAGCT 
ATTTCCTTTT TTGATCCTGA ATCAGATAGC CATTTAGCAC AGCCACTAGT GAAAGTACTA 
TCAGATGCTC AACAGGATGT TCCTGCGTGG TTAGAAGAAA TTGCATTTAG TACCTATGTT 
CCTGGCTTCA GTGGTAGTTC AAGAGGAAAT GTGTTTGCAT CAGTTGATAC TAGAAAGAAT 
TACCAGGGCA AGAGCTCTTT GAACACAGCA GGGTTTTCTT CTTCACAAGC TCCCAATCCA 
GTAGATGATG AGTCATGGGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX4 ( XM_021076578.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020932237.1 Sus scrofa DEAD-box helicase 4 (DDX4), transcript variant X2, mRNA.

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CloneID	OSe240451
Clone ID Related Accession (Same CDS sequence)	XM_021076578.1
Accession Version	XM_021076578.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2214bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	probable ATP-dependent RNA helicase DDX4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010458.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGATG AAGATTGGGA AGCAGAAATA ATCAAACCTC ATATGTCTTC CTATGTTCCC 
GTATTTGAGA AGGATAGGTA TTCTTCTGGA GCAAATGGAG ACACTTATAA TAGGACTCCA 
GCTTCATCAT CAGAAATGGA TGATGGATCT TCTCGAAGAG ATCACTTCAT GAGAAGTGGC 
TTTGCCTCTG GGAGGAGTTT GGGAAACCGG GATCCTGGCG AATCTAATAA AAGAGAGAAT 
ACATCCACAA TGGGTGGTTT TGGTGTTGGA AAGAGTTTTG GAAACAGAGG TTTTTCAAAT 
AACAAGTTTG AAGAAGGTGA TAGCTCTGGT TTCTGGAGAG AGTCTAATAA TGACTGTGAA 
GATAATCAGA CACGGAACAG AGGTTTTTCC AAGAGAGGCG TGACCAACCT CACTAAACTT 
AATGGCTTTC AAGATGGAAA TGATTCAGAA GCCTCAGTGT CGTCCAGAAG AGGTGGAAGA 
GGTAGTTTCC GAGGTTGCCG TGGACGATTT GGTCTAGGAA GTCCAAATAG CGAATATGAA 
CAAGATGAGG GGACCCATCG TACTGGTGGC TTTTTTGGTT CTAGAAGATC AGCATTAAGT 
GGTGCAGGTA ATGGTGACAC TTACCAAAGC AGAAGTGGTA GTGGAAGTGG ACGAGGTGGT 
TACAAAGGTC TAAATGAAGA AGTAATTACA GGCTCTGGAA AGAATTCTTG GAAGTCAGAA 
GCTGAAGGAG GAGAAAGTGG TGACATTCAA GGACCAAAAG TAACCTACAT ACCACCTCCT 
CCCCCTGAGG ATGAGGACTC CATCTTTGCA CATTATCAGA CAGGCATAAA CTTTGACAAA 
TATGATACTA TTCTTGTAGA AGTATCTGGA CATGAGGCAC CGCCAGCAAT TCTGACTTTT 
GAAGAAGCTA ATCTCTGTCA GACACTGAAT AGCAACATTG CTAAAGCTGG TTATACTAAG 
CTTACTCCTG TACAGAAGTA CAGTATTCCT ATTATACTAG GAGGACGAGA TTTGATGGCT 
TGCGCTCAGA CAGGGTCTGG AAAGACTGCA GCCTTTCTCT TGCCAATTTT GGCTCATATG 
ATGCGTGATG GAATAACTGC CAGTCGTTTT AAAGAGCTGC AGGAACCAGA GTGTATTATT 
GTAGCACCAA CTCGAGAATT GATCAATCAG ATCTATTTGG AAGCCAGAAA ATTTTCTTTT 
GGGACTTGTG TAAGAGCTGT TGTTATATAT GGGGGAACCC AGTTGGGGCA TTCAATTCGA 
CAAATAGTAC AAGGCTGTAA TATATTATGT GCTACTCCTG GGAGACTGAT GGATATCATA 
GGTAAAGAAA AGATTGGTCT CAGACAAGTC AAATATTTAG TTTTGGATGA AGCTGATCGC 
ATGCTGGATA TGGGTTTTGG ACCAGAAATG AAGAAGTTAA TTTCCTGCCC AGGAATGCCA 
TCAAAGGAAC AACGTCAAAC ACTTATGTTT AGTGCAACTT TTCCAGAAGA AATTCAAAGG 
TTGGCTGGGG AGTTTTTAAA ATCGAATTAT TTGTTTGTTG CTGTTGGACA AGTGGGCGGA 
GCATGTAGAG ATGTTCAGCA GACCATTCTC CAAGTTGGCC AGTACTCAAA AAGAGAAAAA 
CTTGTTGAAA TTCTACGAAA CATAGGTGAT GAAAGAACTA TGGTCTTTGT AGAAACTAAG 
AAAAAAGCAG ATTTTATTGC CACTTTTCTT TGTCAAGAAA AAATATCAAC AACCAGTATT 
CATGGTGATC GAGAACAGAG AGAGAGAGAG CAAGCTCTTG GAGATTTTCG CTGTGGGAAG 
TGTCCAGTTC TTGTTGCTAC TTCAGTAGCT GCCCGAGGGC TGGATATTGA AAATGTTCAG 
CATGTTATCA ATTTTGATCT TCCTTCCACC ATCGATGAAT ATGTTCATCG AATTGGGCGT 
ACGGGTCGCT GTGGAAATAC TGGCAGAGCT ATTTCCTTTT TTGATCCTGA ATCAGATAGC 
CATTTAGCAC AGCCACTAGT GAAAGTACTA TCAGATGCTC AACAGGATGT TCCTGCGTGG 
TTAGAAGAAA TTGCATTTAG TACCTATGTT CCTGGCTTCA GTGGTAGTTC AAGAGGAAAT 
GTGTTTGCAT CAGTTGATAC TAGAAAGAAT TACCAGGGCA AGAGCTCTTT GAACACAGCA 
GGGTTTTCTT CTTCACAAGC TCCCAATCCA GTAGATGATG AGTCATGGGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020932237.1
CDS	1061..3274
Translation	MGDEDWEAEIIKPHMSSYVPVFEKDRYSSGANGDTYNRTPASSSEMDDGSSRRDHFMRSGFASGRSLGNRDPGESNKRENTSTMGGFGVGKSFGNRGFSNNKFEEGDSSGFWRESNNDCEDNQTRNRGFSKRGVTNLTKLNGFQDGNDSEASVSSRRGGRGSFRGCRGRFGLGSPNSEYEQDEGTHRTGGFFGSRRSALSGAGNGDTYQSRSGSGSGRGGYKGLNEEVITGSGKNSWKSEAEGGESGDIQGPKVTYIPPPPPEDEDSIFAHYQTGINFDKYDTILVEVSGHEAPPAILTFEEANLCQTLNSNIAKAGYTKLTPVQKYSIPIILGGRDLMACAQTGSGKTAAFLLPILAHMMRDGITASRFKELQEPECIIVAPTRELINQIYLEARKFSFGTCVRAVVIYGGTQLGHSIRQIVQGCNILCATPGRLMDIIGKEKIGLRQVKYLVLDEADRMLDMGFGPEMKKLISCPGMPSKEQRQTLMFSATFPEEIQRLAGEFLKSNYLFVAVGQVGGACRDVQQTILQVGQYSKREKLVEILRNIGDERTMVFVETKKKADFIATFLCQEKISTTSIHGDREQREREQALGDFRCGKCPVLVATSVAARGLDIENVQHVINFDLPSTIDEYVHRIGRTGRCGNTGRAISFFDPESDSHLAQPLVKVLSDAQQDVPAWLEEIAFSTYVPGFSGSSRGNVFASVDTRKNYQGKSSLNTAGFSSSQAPNPVDDESWD

Target ORF information:

RefSeq Version	XM_021076578.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa DEAD-box helicase 4 (DDX4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021076578.1

ORF Insert Sequence:

ATGGGAGATG AAGATTGGGA AGCAGAAATA ATCAAACCTC ATATGTCTTC CTATGTTCCC 
GTATTTGAGA AGGATAGGTA TTCTTCTGGA GCAAATGGAG ACACTTATAA TAGGACTCCA 
GCTTCATCAT CAGAAATGGA TGATGGATCT TCTCGAAGAG ATCACTTCAT GAGAAGTGGC 
TTTGCCTCTG GGAGGAGTTT GGGAAACCGG GATCCTGGCG AATCTAATAA AAGAGAGAAT 
ACATCCACAA TGGGTGGTTT TGGTGTTGGA AAGAGTTTTG GAAACAGAGG TTTTTCAAAT 
AACAAGTTTG AAGAAGGTGA TAGCTCTGGT TTCTGGAGAG AGTCTAATAA TGACTGTGAA 
GATAATCAGA CACGGAACAG AGGTTTTTCC AAGAGAGGCG TGACCAACCT CACTAAACTT 
AATGGCTTTC AAGATGGAAA TGATTCAGAA GCCTCAGTGT CGTCCAGAAG AGGTGGAAGA 
GGTAGTTTCC GAGGTTGCCG TGGACGATTT GGTCTAGGAA GTCCAAATAG CGAATATGAA 
CAAGATGAGG GGACCCATCG TACTGGTGGC TTTTTTGGTT CTAGAAGATC AGCATTAAGT 
GGTGCAGGTA ATGGTGACAC TTACCAAAGC AGAAGTGGTA GTGGAAGTGG ACGAGGTGGT 
TACAAAGGTC TAAATGAAGA AGTAATTACA GGCTCTGGAA AGAATTCTTG GAAGTCAGAA 
GCTGAAGGAG GAGAAAGTGG TGACATTCAA GGACCAAAAG TAACCTACAT ACCACCTCCT 
CCCCCTGAGG ATGAGGACTC CATCTTTGCA CATTATCAGA CAGGCATAAA CTTTGACAAA 
TATGATACTA TTCTTGTAGA AGTATCTGGA CATGAGGCAC CGCCAGCAAT TCTGACTTTT 
GAAGAAGCTA ATCTCTGTCA GACACTGAAT AGCAACATTG CTAAAGCTGG TTATACTAAG 
CTTACTCCTG TACAGAAGTA CAGTATTCCT ATTATACTAG GAGGACGAGA TTTGATGGCT 
TGCGCTCAGA CAGGGTCTGG AAAGACTGCA GCCTTTCTCT TGCCAATTTT GGCTCATATG 
ATGCGTGATG GAATAACTGC CAGTCGTTTT AAAGAGCTGC AGGAACCAGA GTGTATTATT 
GTAGCACCAA CTCGAGAATT GATCAATCAG ATCTATTTGG AAGCCAGAAA ATTTTCTTTT 
GGGACTTGTG TAAGAGCTGT TGTTATATAT GGGGGAACCC AGTTGGGGCA TTCAATTCGA 
CAAATAGTAC AAGGCTGTAA TATATTATGT GCTACTCCTG GGAGACTGAT GGATATCATA 
GGTAAAGAAA AGATTGGTCT CAGACAAGTC AAATATTTAG TTTTGGATGA AGCTGATCGC 
ATGCTGGATA TGGGTTTTGG ACCAGAAATG AAGAAGTTAA TTTCCTGCCC AGGAATGCCA 
TCAAAGGAAC AACGTCAAAC ACTTATGTTT AGTGCAACTT TTCCAGAAGA AATTCAAAGG 
TTGGCTGGGG AGTTTTTAAA ATCGAATTAT TTGTTTGTTG CTGTTGGACA AGTGGGCGGA 
GCATGTAGAG ATGTTCAGCA GACCATTCTC CAAGTTGGCC AGTACTCAAA AAGAGAAAAA 
CTTGTTGAAA TTCTACGAAA CATAGGTGAT GAAAGAACTA TGGTCTTTGT AGAAACTAAG 
AAAAAAGCAG ATTTTATTGC CACTTTTCTT TGTCAAGAAA AAATATCAAC AACCAGTATT 
CATGGTGATC GAGAACAGAG AGAGAGAGAG CAAGCTCTTG GAGATTTTCG CTGTGGGAAG 
TGTCCAGTTC TTGTTGCTAC TTCAGTAGCT GCCCGAGGGC TGGATATTGA AAATGTTCAG 
CATGTTATCA ATTTTGATCT TCCTTCCACC ATCGATGAAT ATGTTCATCG AATTGGGCGT 
ACGGGTCGCT GTGGAAATAC TGGCAGAGCT ATTTCCTTTT TTGATCCTGA ATCAGATAGC 
CATTTAGCAC AGCCACTAGT GAAAGTACTA TCAGATGCTC AACAGGATGT TCCTGCGTGG 
TTAGAAGAAA TTGCATTTAG TACCTATGTT CCTGGCTTCA GTGGTAGTTC AAGAGGAAAT 
GTGTTTGCAT CAGTTGATAC TAGAAAGAAT TACCAGGGCA AGAGCTCTTT GAACACAGCA 
GGGTTTTCTT CTTCACAAGC TCCCAATCCA GTAGATGATG AGTCATGGGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX4 ( XM_021076580.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020932239.1 Sus scrofa DEAD-box helicase 4 (DDX4), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OSe240452
Clone ID Related Accession (Same CDS sequence)	XM_021076580.1
Accession Version	XM_021076580.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2160bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	probable ATP-dependent RNA helicase DDX4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010458.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAACG GATTAGAACT GGAAGCCACC ATGGGAGATG AAGATTGGGA AGCAGAAATA 
ATCAAACCTC ATATGTCTTC CTATGTTCCC GTATTTGAGA AGGATAGGTA TTCTTCTGGA 
GCAAATGGAG ACACTTATAA TAGGACTCCA GCTTCATCAT CAGAAATGGA TGATGGATCT 
TCTCGAAGAG ATCACTTCAT GAGAAGTGGC TTTGCCTCTG GGAGGAGTTT GGGAAACCGG 
GATCCTGGCG AATCTAATAA AAGAGAGAAT ACATCCACAA TGGGTGGTTT TGGTGTTGGA 
AAGAGTTTTG GAAACAGAGG TTTTTCAAAT AACAAGTTTG AAGAAGGTGA TAGCTCTGGT 
TTCTGGAGAG GCTTTCAAGA TGGAAATGAT TCAGAAGCCT CAGTGTCGTC CAGAAGAGGT 
GGAAGAGGTA GTTTCCGAGG TTGCCGTGGA CGATTTGGTC TAGGAAGTCC AAATAGCGAA 
TATGAACAAG ATGAGGGGAC CCATCGTACT GGTGGCTTTT TTGGTTCTAG AAGATCAGCA 
TTAAGTGGTG CAGGTAATGG TGACACTTAC CAAAGCAGAA GTGGTAGTGG AAGTGGACGA 
GGTGGTTACA AAGGTCTAAA TGAAGAAGTA ATTACAGGCT CTGGAAAGAA TTCTTGGAAG 
TCAGAAGCTG AAGGAGGAGA AAGTGGTGAC ATTCAAGGAC CAAAAGTAAC CTACATACCA 
CCTCCTCCCC CTGAGGATGA GGACTCCATC TTTGCACATT ATCAGACAGG CATAAACTTT 
GACAAATATG ATACTATTCT TGTAGAAGTA TCTGGACATG AGGCACCGCC AGCAATTCTG 
ACTTTTGAAG AAGCTAATCT CTGTCAGACA CTGAATAGCA ACATTGCTAA AGCTGGTTAT 
ACTAAGCTTA CTCCTGTACA GAAGTACAGT ATTCCTATTA TACTAGGAGG ACGAGATTTG 
ATGGCTTGCG CTCAGACAGG GTCTGGAAAG ACTGCAGCCT TTCTCTTGCC AATTTTGGCT 
CATATGATGC GTGATGGAAT AACTGCCAGT CGTTTTAAAG AGCTGCAGGA ACCAGAGTGT 
ATTATTGTAG CACCAACTCG AGAATTGATC AATCAGATCT ATTTGGAAGC CAGAAAATTT 
TCTTTTGGGA CTTGTGTAAG AGCTGTTGTT ATATATGGGG GAACCCAGTT GGGGCATTCA 
ATTCGACAAA TAGTACAAGG CTGTAATATA TTATGTGCTA CTCCTGGGAG ACTGATGGAT 
ATCATAGGTA AAGAAAAGAT TGGTCTCAGA CAAGTCAAAT ATTTAGTTTT GGATGAAGCT 
GATCGCATGC TGGATATGGG TTTTGGACCA GAAATGAAGA AGTTAATTTC CTGCCCAGGA 
ATGCCATCAA AGGAACAACG TCAAACACTT ATGTTTAGTG CAACTTTTCC AGAAGAAATT 
CAAAGGTTGG CTGGGGAGTT TTTAAAATCG AATTATTTGT TTGTTGCTGT TGGACAAGTG 
GGCGGAGCAT GTAGAGATGT TCAGCAGACC ATTCTCCAAG TTGGCCAGTA CTCAAAAAGA 
GAAAAACTTG TTGAAATTCT ACGAAACATA GGTGATGAAA GAACTATGGT CTTTGTAGAA 
ACTAAGAAAA AAGCAGATTT TATTGCCACT TTTCTTTGTC AAGAAAAAAT ATCAACAACC 
AGTATTCATG GTGATCGAGA ACAGAGAGAG AGAGAGCAAG CTCTTGGAGA TTTTCGCTGT 
GGGAAGTGTC CAGTTCTTGT TGCTACTTCA GTAGCTGCCC GAGGGCTGGA TATTGAAAAT 
GTTCAGCATG TTATCAATTT TGATCTTCCT TCCACCATCG ATGAATATGT TCATCGAATT 
GGGCGTACGG GTCGCTGTGG AAATACTGGC AGAGCTATTT CCTTTTTTGA TCCTGAATCA 
GATAGCCATT TAGCACAGCC ACTAGTGAAA GTACTATCAG ATGCTCAACA GGATGTTCCT 
GCGTGGTTAG AAGAAATTGC ATTTAGTACC TATGTTCCTG GCTTCAGTGG TAGTTCAAGA 
GGAAATGTGT TTGCATCAGT TGATACTAGA AAGAATTACC AGGGCAAGAG CTCTTTGAAC 
ACAGCAGGGT TTTCTTCTTC ACAAGCTCCC AATCCAGTAG ATGATGAGTC ATGGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020932239.1
CDS	4..2163
Translation	MKNGLELEATMGDEDWEAEIIKPHMSSYVPVFEKDRYSSGANGDTYNRTPASSSEMDDGSSRRDHFMRSGFASGRSLGNRDPGESNKRENTSTMGGFGVGKSFGNRGFSNNKFEEGDSSGFWRGFQDGNDSEASVSSRRGGRGSFRGCRGRFGLGSPNSEYEQDEGTHRTGGFFGSRRSALSGAGNGDTYQSRSGSGSGRGGYKGLNEEVITGSGKNSWKSEAEGGESGDIQGPKVTYIPPPPPEDEDSIFAHYQTGINFDKYDTILVEVSGHEAPPAILTFEEANLCQTLNSNIAKAGYTKLTPVQKYSIPIILGGRDLMACAQTGSGKTAAFLLPILAHMMRDGITASRFKELQEPECIIVAPTRELINQIYLEARKFSFGTCVRAVVIYGGTQLGHSIRQIVQGCNILCATPGRLMDIIGKEKIGLRQVKYLVLDEADRMLDMGFGPEMKKLISCPGMPSKEQRQTLMFSATFPEEIQRLAGEFLKSNYLFVAVGQVGGACRDVQQTILQVGQYSKREKLVEILRNIGDERTMVFVETKKKADFIATFLCQEKISTTSIHGDREQREREQALGDFRCGKCPVLVATSVAARGLDIENVQHVINFDLPSTIDEYVHRIGRTGRCGNTGRAISFFDPESDSHLAQPLVKVLSDAQQDVPAWLEEIAFSTYVPGFSGSSRGNVFASVDTRKNYQGKSSLNTAGFSSSQAPNPVDDESWD

Target ORF information:

RefSeq Version	XM_021076580.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa DEAD-box helicase 4 (DDX4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021076580.1

ORF Insert Sequence:

ATGAAGAACG GATTAGAACT GGAAGCCACC ATGGGAGATG AAGATTGGGA AGCAGAAATA 
ATCAAACCTC ATATGTCTTC CTATGTTCCC GTATTTGAGA AGGATAGGTA TTCTTCTGGA 
GCAAATGGAG ACACTTATAA TAGGACTCCA GCTTCATCAT CAGAAATGGA TGATGGATCT 
TCTCGAAGAG ATCACTTCAT GAGAAGTGGC TTTGCCTCTG GGAGGAGTTT GGGAAACCGG 
GATCCTGGCG AATCTAATAA AAGAGAGAAT ACATCCACAA TGGGTGGTTT TGGTGTTGGA 
AAGAGTTTTG GAAACAGAGG TTTTTCAAAT AACAAGTTTG AAGAAGGTGA TAGCTCTGGT 
TTCTGGAGAG GCTTTCAAGA TGGAAATGAT TCAGAAGCCT CAGTGTCGTC CAGAAGAGGT 
GGAAGAGGTA GTTTCCGAGG TTGCCGTGGA CGATTTGGTC TAGGAAGTCC AAATAGCGAA 
TATGAACAAG ATGAGGGGAC CCATCGTACT GGTGGCTTTT TTGGTTCTAG AAGATCAGCA 
TTAAGTGGTG CAGGTAATGG TGACACTTAC CAAAGCAGAA GTGGTAGTGG AAGTGGACGA 
GGTGGTTACA AAGGTCTAAA TGAAGAAGTA ATTACAGGCT CTGGAAAGAA TTCTTGGAAG 
TCAGAAGCTG AAGGAGGAGA AAGTGGTGAC ATTCAAGGAC CAAAAGTAAC CTACATACCA 
CCTCCTCCCC CTGAGGATGA GGACTCCATC TTTGCACATT ATCAGACAGG CATAAACTTT 
GACAAATATG ATACTATTCT TGTAGAAGTA TCTGGACATG AGGCACCGCC AGCAATTCTG 
ACTTTTGAAG AAGCTAATCT CTGTCAGACA CTGAATAGCA ACATTGCTAA AGCTGGTTAT 
ACTAAGCTTA CTCCTGTACA GAAGTACAGT ATTCCTATTA TACTAGGAGG ACGAGATTTG 
ATGGCTTGCG CTCAGACAGG GTCTGGAAAG ACTGCAGCCT TTCTCTTGCC AATTTTGGCT 
CATATGATGC GTGATGGAAT AACTGCCAGT CGTTTTAAAG AGCTGCAGGA ACCAGAGTGT 
ATTATTGTAG CACCAACTCG AGAATTGATC AATCAGATCT ATTTGGAAGC CAGAAAATTT 
TCTTTTGGGA CTTGTGTAAG AGCTGTTGTT ATATATGGGG GAACCCAGTT GGGGCATTCA 
ATTCGACAAA TAGTACAAGG CTGTAATATA TTATGTGCTA CTCCTGGGAG ACTGATGGAT 
ATCATAGGTA AAGAAAAGAT TGGTCTCAGA CAAGTCAAAT ATTTAGTTTT GGATGAAGCT 
GATCGCATGC TGGATATGGG TTTTGGACCA GAAATGAAGA AGTTAATTTC CTGCCCAGGA 
ATGCCATCAA AGGAACAACG TCAAACACTT ATGTTTAGTG CAACTTTTCC AGAAGAAATT 
CAAAGGTTGG CTGGGGAGTT TTTAAAATCG AATTATTTGT TTGTTGCTGT TGGACAAGTG 
GGCGGAGCAT GTAGAGATGT TCAGCAGACC ATTCTCCAAG TTGGCCAGTA CTCAAAAAGA 
GAAAAACTTG TTGAAATTCT ACGAAACATA GGTGATGAAA GAACTATGGT CTTTGTAGAA 
ACTAAGAAAA AAGCAGATTT TATTGCCACT TTTCTTTGTC AAGAAAAAAT ATCAACAACC 
AGTATTCATG GTGATCGAGA ACAGAGAGAG AGAGAGCAAG CTCTTGGAGA TTTTCGCTGT 
GGGAAGTGTC CAGTTCTTGT TGCTACTTCA GTAGCTGCCC GAGGGCTGGA TATTGAAAAT 
GTTCAGCATG TTATCAATTT TGATCTTCCT TCCACCATCG ATGAATATGT TCATCGAATT 
GGGCGTACGG GTCGCTGTGG AAATACTGGC AGAGCTATTT CCTTTTTTGA TCCTGAATCA 
GATAGCCATT TAGCACAGCC ACTAGTGAAA GTACTATCAG ATGCTCAACA GGATGTTCCT 
GCGTGGTTAG AAGAAATTGC ATTTAGTACC TATGTTCCTG GCTTCAGTGG TAGTTCAAGA 
GGAAATGTGT TTGCATCAGT TGATACTAGA AAGAATTACC AGGGCAAGAG CTCTTTGAAC 
ACAGCAGGGT TTTCTTCTTC ACAAGCTCCC AATCCAGTAG ATGATGAGTC ATGGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX4 ( XM_021076581.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020932240.1 Sus scrofa DEAD-box helicase 4 (DDX4), transcript variant X5, mRNA.

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CloneID	OSe240453
Clone ID Related Accession (Same CDS sequence)	XM_021076581.1
Accession Version	XM_021076581.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2079bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	probable ATP-dependent RNA helicase DDX4 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010458.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGATG GATCTTCTCG AAGAGATCAC TTCATGAGAA GTGGCTTTGC CTCTGGGAGG 
AGTTTGGGAA ACCGGGATCC TGGCGAATCT AATAAAAGAG AGAATACATC CACAATGGGT 
GGTTTTGGTG TTGGAAAGAG TTTTGGAAAC AGAGGTTTTT CAAATAACAA GTTTGAAGAA 
GGTGATAGCT CTGGTTTCTG GAGAGAGTCT AATAATGACT GTGAAGATAA TCAGACACGG 
AACAGAGGTT TTTCCAAGAG AGGCGTGACC AACCTCACTA AACTTAATGG CTTTCAAGAT 
GGAAATGATT CAGAAGCCTC AGTGTCGTCC AGAAGAGGTG GAAGAGGTAG TTTCCGAGGT 
TGCCGTGGAC GATTTGGTCT AGGAAGTCCA AATAGCGAAT ATGAACAAGA TGAGGGGACC 
CATCGTACTG GTGGCTTTTT TGGTTCTAGA AGATCAGCAT TAAGTGGTGC AGGTAATGGT 
GACACTTACC AAAGCAGAAG TGGTAGTGGA AGTGGACGAG GTGGTTACAA AGGTCTAAAT 
GAAGAAGTAA TTACAGGCTC TGGAAAGAAT TCTTGGAAGT CAGAAGCTGA AGGAGGAGAA 
AGTGGTGACA TTCAAGGACC AAAAGTAACC TACATACCAC CTCCTCCCCC TGAGGATGAG 
GACTCCATCT TTGCACATTA TCAGACAGGC ATAAACTTTG ACAAATATGA TACTATTCTT 
GTAGAAGTAT CTGGACATGA GGCACCGCCA GCAATTCTGA CTTTTGAAGA AGCTAATCTC 
TGTCAGACAC TGAATAGCAA CATTGCTAAA GCTGGTTATA CTAAGCTTAC TCCTGTACAG 
AAGTACAGTA TTCCTATTAT ACTAGGAGGA CGAGATTTGA TGGCTTGCGC TCAGACAGGG 
TCTGGAAAGA CTGCAGCCTT TCTCTTGCCA ATTTTGGCTC ATATGATGCG TGATGGAATA 
ACTGCCAGTC GTTTTAAAGA GCTGCAGGAA CCAGAGTGTA TTATTGTAGC ACCAACTCGA 
GAATTGATCA ATCAGATCTA TTTGGAAGCC AGAAAATTTT CTTTTGGGAC TTGTGTAAGA 
GCTGTTGTTA TATATGGGGG AACCCAGTTG GGGCATTCAA TTCGACAAAT AGTACAAGGC 
TGTAATATAT TATGTGCTAC TCCTGGGAGA CTGATGGATA TCATAGGTAA AGAAAAGATT 
GGTCTCAGAC AAGTCAAATA TTTAGTTTTG GATGAAGCTG ATCGCATGCT GGATATGGGT 
TTTGGACCAG AAATGAAGAA GTTAATTTCC TGCCCAGGAA TGCCATCAAA GGAACAACGT 
CAAACACTTA TGTTTAGTGC AACTTTTCCA GAAGAAATTC AAAGGTTGGC TGGGGAGTTT 
TTAAAATCGA ATTATTTGTT TGTTGCTGTT GGACAAGTGG GCGGAGCATG TAGAGATGTT 
CAGCAGACCA TTCTCCAAGT TGGCCAGTAC TCAAAAAGAG AAAAACTTGT TGAAATTCTA 
CGAAACATAG GTGATGAAAG AACTATGGTC TTTGTAGAAA CTAAGAAAAA AGCAGATTTT 
ATTGCCACTT TTCTTTGTCA AGAAAAAATA TCAACAACCA GTATTCATGG TGATCGAGAA 
CAGAGAGAGA GAGAGCAAGC TCTTGGAGAT TTTCGCTGTG GGAAGTGTCC AGTTCTTGTT 
GCTACTTCAG TAGCTGCCCG AGGGCTGGAT ATTGAAAATG TTCAGCATGT TATCAATTTT 
GATCTTCCTT CCACCATCGA TGAATATGTT CATCGAATTG GGCGTACGGG TCGCTGTGGA 
AATACTGGCA GAGCTATTTC CTTTTTTGAT CCTGAATCAG ATAGCCATTT AGCACAGCCA 
CTAGTGAAAG TACTATCAGA TGCTCAACAG GATGTTCCTG CGTGGTTAGA AGAAATTGCA 
TTTAGTACCT ATGTTCCTGG CTTCAGTGGT AGTTCAAGAG GAAATGTGTT TGCATCAGTT 
GATACTAGAA AGAATTACCA GGGCAAGAGC TCTTTGAACA CAGCAGGGTT TTCTTCTTCA 
CAAGCTCCCA ATCCAGTAGA TGATGAGTCA TGGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020932240.1
CDS	176..2254
Translation	MDDGSSRRDHFMRSGFASGRSLGNRDPGESNKRENTSTMGGFGVGKSFGNRGFSNNKFEEGDSSGFWRESNNDCEDNQTRNRGFSKRGVTNLTKLNGFQDGNDSEASVSSRRGGRGSFRGCRGRFGLGSPNSEYEQDEGTHRTGGFFGSRRSALSGAGNGDTYQSRSGSGSGRGGYKGLNEEVITGSGKNSWKSEAEGGESGDIQGPKVTYIPPPPPEDEDSIFAHYQTGINFDKYDTILVEVSGHEAPPAILTFEEANLCQTLNSNIAKAGYTKLTPVQKYSIPIILGGRDLMACAQTGSGKTAAFLLPILAHMMRDGITASRFKELQEPECIIVAPTRELINQIYLEARKFSFGTCVRAVVIYGGTQLGHSIRQIVQGCNILCATPGRLMDIIGKEKIGLRQVKYLVLDEADRMLDMGFGPEMKKLISCPGMPSKEQRQTLMFSATFPEEIQRLAGEFLKSNYLFVAVGQVGGACRDVQQTILQVGQYSKREKLVEILRNIGDERTMVFVETKKKADFIATFLCQEKISTTSIHGDREQREREQALGDFRCGKCPVLVATSVAARGLDIENVQHVINFDLPSTIDEYVHRIGRTGRCGNTGRAISFFDPESDSHLAQPLVKVLSDAQQDVPAWLEEIAFSTYVPGFSGSSRGNVFASVDTRKNYQGKSSLNTAGFSSSQAPNPVDDESWD

Target ORF information:

RefSeq Version	XM_021076581.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa DEAD-box helicase 4 (DDX4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021076581.1

ORF Insert Sequence:

ATGGATGATG GATCTTCTCG AAGAGATCAC TTCATGAGAA GTGGCTTTGC CTCTGGGAGG 
AGTTTGGGAA ACCGGGATCC TGGCGAATCT AATAAAAGAG AGAATACATC CACAATGGGT 
GGTTTTGGTG TTGGAAAGAG TTTTGGAAAC AGAGGTTTTT CAAATAACAA GTTTGAAGAA 
GGTGATAGCT CTGGTTTCTG GAGAGAGTCT AATAATGACT GTGAAGATAA TCAGACACGG 
AACAGAGGTT TTTCCAAGAG AGGCGTGACC AACCTCACTA AACTTAATGG CTTTCAAGAT 
GGAAATGATT CAGAAGCCTC AGTGTCGTCC AGAAGAGGTG GAAGAGGTAG TTTCCGAGGT 
TGCCGTGGAC GATTTGGTCT AGGAAGTCCA AATAGCGAAT ATGAACAAGA TGAGGGGACC 
CATCGTACTG GTGGCTTTTT TGGTTCTAGA AGATCAGCAT TAAGTGGTGC AGGTAATGGT 
GACACTTACC AAAGCAGAAG TGGTAGTGGA AGTGGACGAG GTGGTTACAA AGGTCTAAAT 
GAAGAAGTAA TTACAGGCTC TGGAAAGAAT TCTTGGAAGT CAGAAGCTGA AGGAGGAGAA 
AGTGGTGACA TTCAAGGACC AAAAGTAACC TACATACCAC CTCCTCCCCC TGAGGATGAG 
GACTCCATCT TTGCACATTA TCAGACAGGC ATAAACTTTG ACAAATATGA TACTATTCTT 
GTAGAAGTAT CTGGACATGA GGCACCGCCA GCAATTCTGA CTTTTGAAGA AGCTAATCTC 
TGTCAGACAC TGAATAGCAA CATTGCTAAA GCTGGTTATA CTAAGCTTAC TCCTGTACAG 
AAGTACAGTA TTCCTATTAT ACTAGGAGGA CGAGATTTGA TGGCTTGCGC TCAGACAGGG 
TCTGGAAAGA CTGCAGCCTT TCTCTTGCCA ATTTTGGCTC ATATGATGCG TGATGGAATA 
ACTGCCAGTC GTTTTAAAGA GCTGCAGGAA CCAGAGTGTA TTATTGTAGC ACCAACTCGA 
GAATTGATCA ATCAGATCTA TTTGGAAGCC AGAAAATTTT CTTTTGGGAC TTGTGTAAGA 
GCTGTTGTTA TATATGGGGG AACCCAGTTG GGGCATTCAA TTCGACAAAT AGTACAAGGC 
TGTAATATAT TATGTGCTAC TCCTGGGAGA CTGATGGATA TCATAGGTAA AGAAAAGATT 
GGTCTCAGAC AAGTCAAATA TTTAGTTTTG GATGAAGCTG ATCGCATGCT GGATATGGGT 
TTTGGACCAG AAATGAAGAA GTTAATTTCC TGCCCAGGAA TGCCATCAAA GGAACAACGT 
CAAACACTTA TGTTTAGTGC AACTTTTCCA GAAGAAATTC AAAGGTTGGC TGGGGAGTTT 
TTAAAATCGA ATTATTTGTT TGTTGCTGTT GGACAAGTGG GCGGAGCATG TAGAGATGTT 
CAGCAGACCA TTCTCCAAGT TGGCCAGTAC TCAAAAAGAG AAAAACTTGT TGAAATTCTA 
CGAAACATAG GTGATGAAAG AACTATGGTC TTTGTAGAAA CTAAGAAAAA AGCAGATTTT 
ATTGCCACTT TTCTTTGTCA AGAAAAAATA TCAACAACCA GTATTCATGG TGATCGAGAA 
CAGAGAGAGA GAGAGCAAGC TCTTGGAGAT TTTCGCTGTG GGAAGTGTCC AGTTCTTGTT 
GCTACTTCAG TAGCTGCCCG AGGGCTGGAT ATTGAAAATG TTCAGCATGT TATCAATTTT 
GATCTTCCTT CCACCATCGA TGAATATGTT CATCGAATTG GGCGTACGGG TCGCTGTGGA 
AATACTGGCA GAGCTATTTC CTTTTTTGAT CCTGAATCAG ATAGCCATTT AGCACAGCCA 
CTAGTGAAAG TACTATCAGA TGCTCAACAG GATGTTCCTG CGTGGTTAGA AGAAATTGCA 
TTTAGTACCT ATGTTCCTGG CTTCAGTGGT AGTTCAAGAG GAAATGTGTT TGCATCAGTT 
GATACTAGAA AGAATTACCA GGGCAAGAGC TCTTTGAACA CAGCAGGGTT TTCTTCTTCA 
CAAGCTCCCA ATCCAGTAGA TGATGAGTCA TGGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Stage-specific expression of DDX4 and c-kit at different developmental stages of the porcine testis.
Animal reproduction science19018-26(2018 Mar)
Lee R,Lee WY,Park HJ,Ha WT,Woo JS,Chung HJ,Lee JH,Hong K,Song H

Cell-surface DEAD-box polypeptide 4-immunoreactive cells and gonocytes are two distinct populations in postnatal porcine testes.
Biology of reproduction90(4)82(2014 Apr)
Kakiuchi K,Tsuda A,Goto Y,Shimada T,Taniguchi K,Takagishi K,Kubota H

Characterization of pig vasa homolog gene and specific expression in germ cell lineage.
Molecular reproduction and development72(3)320-8(2005 Nov)
Lee GS,Kim HS,Lee SH,Kang MS,Kim DY,Lee CK,Kang SK,Lee BC,Hwang WS

Expression and intracellular localization of mouse Vasa-homologue protein during germ cell development.
Mechanisms of development93(1-2)139-49(2000 May)
Toyooka Y,Tsunekawa N,Takahashi Y,Matsui Y,Satoh M,Noce T

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DDX4 cDNA ORF clone, Sus scrofa(pig)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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