LOC756277 cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC756277
Entrez Gene ID	756277
Full Name	epididymis-specific alpha-mannosidase
Gene Type	protein-coding
Organism	Strongylocentrotus purpuratus(purple sea urchin)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011675572.1	XP_011673874.1	epididymis-specific alpha-mannosidase
XM_011675572.2	XP_011673874.2	epididymis-specific alpha-mannosidase

Pathways from BioSystems
Gene Ontology

The following LOC756277 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC756277 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSd29074	XM_011675572.1 Latest version!	Strongylocentrotus purpuratus epididymis-specific alpha-mannosidase (LOC756277), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OSd64639	XM_011675572.2 Latest version!	Strongylocentrotus purpuratus epididymis-specific alpha-mannosidase (LOC756277), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC756277 ( XM_011675572.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_011673874.1 Strongylocentrotus purpuratus epididymis-specific alpha-mannosidase (LOC756277), mRNA.

Price & Availability↑

CloneID	OSd29074
Clone ID Related Accession (Same CDS sequence)	XM_011675572.1
Accession Version	XM_011675572.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1427040000000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	epididymis-specific alpha-mannosidase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011989134.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Strongylocentrotus purpuratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTCC TGAAAAGGAA TTTCTTCATC GTAATAACCA TCTCTGTCAA CTTCTTGATT 
GGTTCCGGCG TGGTGACCGT CCAGGGACTT GGCGTCGAAA GAGAGGACGA AGTCGACAAT 
GCATATAGTG ATGGCGATAC CCTGAATGTT TATGTGATAC CCCATAGTCA CATGGACGTT 
GGATGGGTTT ACACAGTCAA GGAGAGTATG CACGCTTATG CCGCCAATGT CTACACCACA 
GTGATAGCTG AACTTGGTCG CAACCCTGCC CGCCGATTTA TTGCTGTAGA ACAGGAGTTC 
TTCAGGCTCT GGTGGGATGG CGTAGCCACA GAAAAACAAA AGAGTCAAAC TCGAAAGTTT 
GTTTCAAATG GACAGTTGGA GTTTATCATT GGTGGTCAGG TGATGCACGA TGAAGCAGTA 
ACTGAACTTA GCGCCATTAT ACAACAAATG ACAGAAGGAC ACGCCTTTAT CTACGAGACG 
TTTGGTGTTC GGCCCCGGTT CGGTTGGCAT GTTGATCCAT TCGGCGCAAC GGCGGCCACG 
CCCACTCTCT TTGCACTGAT GGGGTTTAAT GCCCATCTGA CGTCACGAAT AAATTATGAT 
CAGAAAGCCG CCATGATGAA GGCAAAGGGA CTCCAGTTTG TATGGCAAGC CTCCCCATCT 
CTGGGCTCAT CACAGAGCAT GTTCACACAC GTCATGGATA CGTATAGCTA CTGTGCTCCC 
ATGGAGCATC CGCCATTTTC AAACAGATCT GCATATATGT GGAATGGCGT AGCCAAGTTC 
CCAGATCCGC CAGCCGACGG TATCTACCCC AACATGAGCG ACCCAGTGAC GACACAAAAC 
ATGGGACAGT ATGCGGACTT CATGGTGGCC AACATTCGGA AACGAGCTGC GTGGTTCAAG 
ACCCCGCATC TACTCTGGCC ATGGGGATGT GATAAGCAGT TCTTCAATGC CACCGACCAG 
TATGAAAATA TGGAACGTCT TCTAGTGCAT ATAAACCAAC AGCGATCTAA ATACAATATC 
AAAATCCGAC AGGCGACACT CGGGCAGTTC TTCACCGCCC TTCACAACAG TGACACTACG 
TGGGACGTCA AGAACAGTGG AGATTTTATC CCTTATTCGA CCGCACGCGA GGAAGCTTGG 
ACCGGATTCT ACACCTCACG TATTGAGCTG AAGGGGATAG CCCGGGCGAG CCAGGCTACT 
CTAAGAGCTG GAGAGACCCT CTTCTCTCTG TATACCCATC TCTTTAACAT CTCAAGGACC 
ATTAATACCA CCAGCGCACT GGAGAAGATC AAGAAGCTAA GATGGGCTAC TGCCGAGGTG 
CAACATCACG ACGGCGTCAC GGGAACAGAA GCCCCTGTGG TGCGTGACAT GTATATCAGT 
CACCTACGTG ACGGCAGTCA AGCTACCATA AGTGTGCTGA CGGATATACT TAATGAATTG 
ACGCAAGTTA ACGCAAGGGA GACGGAACAC CACAACGAAA GATATAATGT ACTCGGGTTA 
AAATCAGAAA TTAGTGAAGG CAAGAGCACT CTAACCTACG CGGTCTACAA CTCTCTCGGA 
TGGACCGTTC GACGCCTAGT ACGAATTGAA GTTAACTCGC CCAATGTGAC GGTCCTTGAC 
GCACGGGGTC AACCCGTCAT TAGCCAGGTA AATCCGCCAT CGAATGGGAG TCAACTGTAC 
CAACTTTACT TCTTTGCAAC CATTCCACCT ATCGGCTACC AAATATTCTT CCTTTTATTT 
ATCAACGAAA ACCAACGTCT TTTACAAAGC CAGTGTCCCA CTGTGAGAAG TTACGGTGCC 
GCCACCGAGG ATGCGGGAGG CATGGATGGA GGAGAAGGGC GTGGTGATTC AGAAGGAGAT 
GAAATATCGA TCGAGAATGG ATGCTACAAA ATATCTTTTG ACCGTTCCAC GAATGCACTG 
TCGTCGATAA CAAACAAACA AAACGGCAAG ACCACTACAA TGAGCGCGGG ATTCCTGGAG 
TACCATGTCT TCCACGACGT ATTGTATGGC CAAACGTCCA ACAACTACGT ATTCAGACCC 
TACGGACCTG CCACGGGAAT CAGCAGCGTC AGGACTCAAC TTAATGTCAG CACGGGTGTC 
CTCGTAAACG AGACAACGCA ACATTTCTAT GGGCTGTTCG ATGTGGAAAG TCGCTATTCA 
GTGACCATGC GATTGGTCAA CGATGCAAGT GGACATCCTG ATAAGCTGTC GTGCAGCCAT 
ATTGAAGTGG ACTTCAAAGT TGGTCCTCTT TCTATGAACA AAGAACTCGT TTACAGGTTT 
TCTACATTGC TAAAGTCTAA TGGTAATCTG TACGCTGATG TTAATGCCTT CCAAACTATG 
AATAGGTCTA GGCGCCACGG TGCAACCATT GCACAGAACT TCTACCCAAT GGTAAGCTCG 
GCCTACATAG AGGATGCTTC CAACGATGAG AGATTGATGC TGCTCTCTGA GAGGAGCCAT 
GGAGTCAGCA GTCAGAGTGA TGGAGATATC GAGGTCATGT TGCATCGCAG AGTAGTCAAC 
AACGAGTTCG AGACCCACAA TTACAACCTC ACCTTGGAGG AACCTTCTGT AGCAACATCC 
ACGTTCTGGC TGTTGCTAGG CAACGCATCC ACGACATCGG AGCTTCGTCA TCGCAGCTGG 
CTTCAGCTCG AGAACCCACC GGTCGCTGTA CAGCTCGGGT CAGGATGGTC GTCTGTGAAG 
GAAGTAAACT GGTCAGCCTT GCAATCCCGG AGGTTTCATT CTGCTATAGC AAATGTGTTA 
CCAAAGAACA TTCATCTTCT CACCTTCAAA ACACCAGGCT GGAATTACAA CGTCTCTGCC 
AAAACCAGTC AATCTACGGG AGGATCCCCA CTCAATCTTA GCCGAATTCT GCTGCGGCTC 
CAGCACCTGT ACGAAGTAGG TGAACACCCC ATACTGTCCA GGAACGTCAC CGTAGATCTT 
CAAATGATTT TATCACCATT GGGGATGATA AAGACAGTGG ATGAGCGTAG CCTGACGGGT 
ATCTGGAACA TCACTCAGAT GAACAGGTGG AAGTGGAGAA GAGCAGATGA AGGTCGGTCT 
CAATCACCTG ACATGTCGAT CAGCAACACG ATTACCCACG CAGAACCAAC AACAACACTA 
ACCACAACCA CAGAACCAAC CACAACCACA GCGCCAGCGC CAACAACAAC TGCCACACCA 
CCTAGCTTTA CCTACACACT ACAACCACAG GAAATTAAGA CCTTCTTTGT CATCATGAAT 
GCACCGACCG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011673874.1
CDS	139..3393
Translation	MALLKRNFFIVITISVNFLIGSGVVTVQGLGVEREDEVDNAYSDGDTLNVYVIPHSHMDVGWVYTVKESMHAYAANVYTTVIAELGRNPARRFIAVEQEFFRLWWDGVATEKQKSQTRKFVSNGQLEFIIGGQVMHDEAVTELSAIIQQMTEGHAFIYETFGVRPRFGWHVDPFGATAATPTLFALMGFNAHLTSRINYDQKAAMMKAKGLQFVWQASPSLGSSQSMFTHVMDTYSYCAPMEHPPFSNRSAYMWNGVAKFPDPPADGIYPNMSDPVTTQNMGQYADFMVANIRKRAAWFKTPHLLWPWGCDKQFFNATDQYENMERLLVHINQQRSKYNIKIRQATLGQFFTALHNSDTTWDVKNSGDFIPYSTAREEAWTGFYTSRIELKGIARASQATLRAGETLFSLYTHLFNISRTINTTSALEKIKKLRWATAEVQHHDGVTGTEAPVVRDMYISHLRDGSQATISVLTDILNELTQVNARETEHHNERYNVLGLKSEISEGKSTLTYAVYNSLGWTVRRLVRIEVNSPNVTVLDARGQPVISQVNPPSNGSQLYQLYFFATIPPIGYQIFFLLFINENQRLLQSQCPTVRSYGAATEDAGGMDGGEGRGDSEGDEISIENGCYKISFDRSTNALSSITNKQNGKTTTMSAGFLEYHVFHDVLYGQTSNNYVFRPYGPATGISSVRTQLNVSTGVLVNETTQHFYGLFDVESRYSVTMRLVNDASGHPDKLSCSHIEVDFKVGPLSMNKELVYRFSTLLKSNGNLYADVNAFQTMNRSRRHGATIAQNFYPMVSSAYIEDASNDERLMLLSERSHGVSSQSDGDIEVMLHRRVVNNEFETHNYNLTLEEPSVATSTFWLLLGNASTTSELRHRSWLQLENPPVAVQLGSGWSSVKEVNWSALQSRRFHSAIANVLPKNIHLLTFKTPGWNYNVSAKTSQSTGGSPLNLSRILLRLQHLYEVGEHPILSRNVTVDLQMILSPLGMIKTVDERSLTGIWNITQMNRWKWRRADEGRSQSPDMSISNTITHAEPTTTLTTTTEPTTTTAPAPTTTATPPSFTYTLQPQEIKTFFVIMNAPTE

Target ORF information:

RefSeq Version	XM_011675572.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus epididymis-specific alpha-mannosidase (LOC756277), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011675572.1

ORF Insert Sequence:

ATGGCGCTCC TGAAAAGGAA TTTCTTCATC GTAATAACCA TCTCTGTCAA CTTCTTGATT 
GGTTCCGGCG TGGTGACCGT CCAGGGACTT GGCGTCGAAA GAGAGGACGA AGTCGACAAT 
GCATATAGTG ATGGCGATAC CCTGAATGTT TATGTGATAC CCCATAGTCA CATGGACGTT 
GGATGGGTTT ACACAGTCAA GGAGAGTATG CACGCTTATG CCGCCAATGT CTACACCACA 
GTGATAGCTG AACTTGGTCG CAACCCTGCC CGCCGATTTA TTGCTGTAGA ACAGGAGTTC 
TTCAGGCTCT GGTGGGATGG CGTAGCCACA GAAAAACAAA AGAGTCAAAC TCGAAAGTTT 
GTTTCAAATG GACAGTTGGA GTTTATCATT GGTGGTCAGG TGATGCACGA TGAAGCAGTA 
ACTGAACTTA GCGCCATTAT ACAACAAATG ACAGAAGGAC ACGCCTTTAT CTACGAGACG 
TTTGGTGTTC GGCCCCGGTT CGGTTGGCAT GTTGATCCAT TCGGCGCAAC GGCGGCCACG 
CCCACTCTCT TTGCACTGAT GGGGTTTAAT GCCCATCTGA CGTCACGAAT AAATTATGAT 
CAGAAAGCCG CCATGATGAA GGCAAAGGGA CTCCAGTTTG TATGGCAAGC CTCCCCATCT 
CTGGGCTCAT CACAGAGCAT GTTCACACAC GTCATGGATA CGTATAGCTA CTGTGCTCCC 
ATGGAGCATC CGCCATTTTC AAACAGATCT GCATATATGT GGAATGGCGT AGCCAAGTTC 
CCAGATCCGC CAGCCGACGG TATCTACCCC AACATGAGCG ACCCAGTGAC GACACAAAAC 
ATGGGACAGT ATGCGGACTT CATGGTGGCC AACATTCGGA AACGAGCTGC GTGGTTCAAG 
ACCCCGCATC TACTCTGGCC ATGGGGATGT GATAAGCAGT TCTTCAATGC CACCGACCAG 
TATGAAAATA TGGAACGTCT TCTAGTGCAT ATAAACCAAC AGCGATCTAA ATACAATATC 
AAAATCCGAC AGGCGACACT CGGGCAGTTC TTCACCGCCC TTCACAACAG TGACACTACG 
TGGGACGTCA AGAACAGTGG AGATTTTATC CCTTATTCGA CCGCACGCGA GGAAGCTTGG 
ACCGGATTCT ACACCTCACG TATTGAGCTG AAGGGGATAG CCCGGGCGAG CCAGGCTACT 
CTAAGAGCTG GAGAGACCCT CTTCTCTCTG TATACCCATC TCTTTAACAT CTCAAGGACC 
ATTAATACCA CCAGCGCACT GGAGAAGATC AAGAAGCTAA GATGGGCTAC TGCCGAGGTG 
CAACATCACG ACGGCGTCAC GGGAACAGAA GCCCCTGTGG TGCGTGACAT GTATATCAGT 
CACCTACGTG ACGGCAGTCA AGCTACCATA AGTGTGCTGA CGGATATACT TAATGAATTG 
ACGCAAGTTA ACGCAAGGGA GACGGAACAC CACAACGAAA GATATAATGT ACTCGGGTTA 
AAATCAGAAA TTAGTGAAGG CAAGAGCACT CTAACCTACG CGGTCTACAA CTCTCTCGGA 
TGGACCGTTC GACGCCTAGT ACGAATTGAA GTTAACTCGC CCAATGTGAC GGTCCTTGAC 
GCACGGGGTC AACCCGTCAT TAGCCAGGTA AATCCGCCAT CGAATGGGAG TCAACTGTAC 
CAACTTTACT TCTTTGCAAC CATTCCACCT ATCGGCTACC AAATATTCTT CCTTTTATTT 
ATCAACGAAA ACCAACGTCT TTTACAAAGC CAGTGTCCCA CTGTGAGAAG TTACGGTGCC 
GCCACCGAGG ATGCGGGAGG CATGGATGGA GGAGAAGGGC GTGGTGATTC AGAAGGAGAT 
GAAATATCGA TCGAGAATGG ATGCTACAAA ATATCTTTTG ACCGTTCCAC GAATGCACTG 
TCGTCGATAA CAAACAAACA AAACGGCAAG ACCACTACAA TGAGCGCGGG ATTCCTGGAG 
TACCATGTCT TCCACGACGT ATTGTATGGC CAAACGTCCA ACAACTACGT ATTCAGACCC 
TACGGACCTG CCACGGGAAT CAGCAGCGTC AGGACTCAAC TTAATGTCAG CACGGGTGTC 
CTCGTAAACG AGACAACGCA ACATTTCTAT GGGCTGTTCG ATGTGGAAAG TCGCTATTCA 
GTGACCATGC GATTGGTCAA CGATGCAAGT GGACATCCTG ATAAGCTGTC GTGCAGCCAT 
ATTGAAGTGG ACTTCAAAGT TGGTCCTCTT TCTATGAACA AAGAACTCGT TTACAGGTTT 
TCTACATTGC TAAAGTCTAA TGGTAATCTG TACGCTGATG TTAATGCCTT CCAAACTATG 
AATAGGTCTA GGCGCCACGG TGCAACCATT GCACAGAACT TCTACCCAAT GGTAAGCTCG 
GCCTACATAG AGGATGCTTC CAACGATGAG AGATTGATGC TGCTCTCTGA GAGGAGCCAT 
GGAGTCAGCA GTCAGAGTGA TGGAGATATC GAGGTCATGT TGCATCGCAG AGTAGTCAAC 
AACGAGTTCG AGACCCACAA TTACAACCTC ACCTTGGAGG AACCTTCTGT AGCAACATCC 
ACGTTCTGGC TGTTGCTAGG CAACGCATCC ACGACATCGG AGCTTCGTCA TCGCAGCTGG 
CTTCAGCTCG AGAACCCACC GGTCGCTGTA CAGCTCGGGT CAGGATGGTC GTCTGTGAAG 
GAAGTAAACT GGTCAGCCTT GCAATCCCGG AGGTTTCATT CTGCTATAGC AAATGTGTTA 
CCAAAGAACA TTCATCTTCT CACCTTCAAA ACACCAGGCT GGAATTACAA CGTCTCTGCC 
AAAACCAGTC AATCTACGGG AGGATCCCCA CTCAATCTTA GCCGAATTCT GCTGCGGCTC 
CAGCACCTGT ACGAAGTAGG TGAACACCCC ATACTGTCCA GGAACGTCAC CGTAGATCTT 
CAAATGATTT TATCACCATT GGGGATGATA AAGACAGTGG ATGAGCGTAG CCTGACGGGT 
ATCTGGAACA TCACTCAGAT GAACAGGTGG AAGTGGAGAA GAGCAGATGA AGGTCGGTCT 
CAATCACCTG ACATGTCGAT CAGCAACACG ATTACCCACG CAGAACCAAC AACAACACTA 
ACCACAACCA CAGAACCAAC CACAACCACA GCGCCAGCGC CAACAACAAC TGCCACACCA 
CCTAGCTTTA CCTACACACT ACAACCACAG GAAATTAAGA CCTTCTTTGT CATCATGAAT 
GCACCGACCG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC756277 ( XM_011675572.2 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_011673874.2 Strongylocentrotus purpuratus epididymis-specific alpha-mannosidase (LOC756277), mRNA.

Price & Availability↑

CloneID	OSd64639
Clone ID Related Accession (Same CDS sequence)	XM_011675572.2
Accession Version	XM_011675572.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569427200000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	epididymis-specific alpha-mannosidase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022145611.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 27, 2019 this sequence version replaced XM_011675572.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Strongylocentrotus purpuratus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTCC TGAAAAGGAA TTTCTTCATC GTAATAACCA TCTCTGTCAA CTTCTTGATT 
GGTTCCGGCG TGGTGACCGT CCAGGGACTT GGCGTCGAAA GAGAGGACGA AGTCGACAAT 
GCATATAGTG ATGGCGATAC CCTGAATGTT TATGTGATAC CCCATAGTCA CATGGACGTT 
GGATGGGTTT ACACAGTCAA GGAGAGTATG CACGCTTATG CCGCCAATGT CTACACCACA 
GTGATAGCTG AACTTGGTCG CAACCCTGCC CGCCGATTTA TTGCTGTAGA ACAGGAGTTC 
TTCAGGCTCT GGTGGGATGG CGTAGCCACA GAAAAACAAA AGAGTCAAAC TCGAAAGTTT 
GTTTCAAATG GACAGTTGGA GTTTATCATT GGTGGTCAGG TGATGCACGA TGAAGCAGTA 
ACTGAACTTA GCGCCATTAT ACAACAAATG ACAGAAGGAC ACGCCTTTAT CTACGAGACG 
TTTGGTGTTC GGCCCCGGTT CGGTTGGCAT GTTGATCCAT TCGGCGCAAC GGCGGCCACG 
CCCACTCTCT TTGCACTGAT GGGGTTTAAT GCCCATCTGA CGTCACGAAT AAATTATGAT 
CAGAAAGCCG CCATGATGAA GGCAAAGGGA CTCCAGTTTG TATGGCAAGC CTCCCCATCT 
CTGGGCTCAT CACAGAGCAT GTTCACACAC GTCATGGATA CGTATAGCTA CTGTGCTCCC 
ATGGAGCATC CGCCATTTTC AAACAGATCT GCATATATGT GGAATGGCGT AGCCAAGTTC 
CCAGATCCGC CAGCCGACGG TATCTACCCC AACATGAGCG ACCCAGTGAC GACACAAAAC 
ATGGGACAGT ATGCGGACTT CATGGTGGCC AACATTCGGA AACGAGCTGC GTGGTTCAAG 
ACCCCGCATC TACTCTGGCC ATGGGGATGT GATAAGCAGT TCTTCAATGC CACCGACCAG 
TATGAAAATA TGGAACGTCT TCTAGTGCAT ATAAACCAAC AGCGATCTAA ATACAATATC 
AAAATCCGAC AGGCGACACT CGGGCAGTTC TTCACCGCCC TTCACAACAG TGACACTACG 
TGGGACGTCA AGAACAGTGG AGATTTTATC CCTTATTCGA CCGCACGCGA GGAAGCTTGG 
ACCGGATTCT ACACCTCACG TATTGAGCTG AAGGGGATAG CCCGGGCGAG CCAGGCTACT 
CTAAGAGCTG GAGAGACCCT CTTCTCTCTG TATACCCATC TCTTTAACAT CTCAAGGACC 
ATTAATACCA CCAGCGCACT GGAGAAGATC AAGAAGCTAA GATGGGCTAC TGCCGAGGTG 
CAACATCACG ACGGCGTCAC GGGAACAGAA GCCCCTGTGG TGCGTGACAT GTATATCAGT 
CACCTACGTG ACGGCAGTCA AGCTACCATA AGTGTGCTGA CGGATATACT TAATGAATTG 
ACGCAAGTTA ACGCAAGGGA GACGGAACAC CACAACGAAA GATATAATGT ACTCGGGTTA 
AAATCAGAAA TTAGTGAAGG CAAGAGCACT CTAACCTACG CGGTCTACAA CTCTCTCGGA 
TGGACCGTTC GACGCCTAGT ACGAATTGAA GTTAACTCGC CCAATGTGAC GGTCCTTGAC 
GCACGGGGTC AACCCGTCAT TAGCCAGGTA AATCCGCCGT CGAATGGGAG TCAACTGTAC 
CAACTTTACT TCTTTGCAAC CATTCCACCT ATCGGCTACC AAATATTCGT CCTTTTATTT 
ATCAACGAAA ACCAACGTCT TTTACAAAGC CAGTGTCCCA CTGTGAGAAG TTACGGTGCC 
GCCACCGAGG ATGCGGGAGG CATGGATGAA GGAGAAGGGC GCGGTGATTC AGAAGGAGAT 
GAAATATCGA TCGAGAATGA ATGCTACAAA ATATCTTTTG ACCGTTCCAC GAATGCACTG 
TCGTCGATAA CAAACAAACA AAACGGCAAG ACCACTACAA TGAGCGCGGG ATTCCTGGAG 
TACCATGTCT TCCACGACGT ATTGTACGGC CAAACGTCCA ACAACTACGT ATTCAGACCC 
TACGGACCTG CCACGGTAAT CGGCAGCGTC AGGACTCAAC TTAATGTCAG CACGGGTGTC 
CTCGTAAACG AGACAACACA ACATTTCTAT GGGCTGTTCG ATGTGGAAAG TCGCTATTCA 
GTGACCATGC GATTGATCAA CGATGCAAGT GGACATCCTG ATAAGCTGTC GTGCAGCCAT 
ATTGAAGTGG ACTTCAAAGT TGGTCCTCTT TCTATGAACA AGGAACTCGT TTACAGGTTT 
TCTACATTGC TAAAGTCTAA TGGTAATCTG TACGCTGATG TTAATGCCTT CCAAACTATG 
AATAGGTCTA GGCGCCACGG TGCAACCATT GCACAGAACT TCTACCCAAT GGTAAGCTCG 
GCCTACATAG AGGATGCTTC CAACGATGAG AGATTGATGC TGCTCTCTGA GAGGAGCCAT 
GGAGTCAGCA GTCAGAGTGA TGGAGATATC GAGGTCATGT TGCATCGCAG AGTAGTCAAC 
AACGAGTTCG AGACCCACAA TTACAACCTC ACCTTGGAGG AACCTTCTGT AGCAACATCC 
ACGTTCTGGC TGTTGCTAGG CAACGCATCC ACGACATCGG AGCTTCGTCA TCGCAGCTGG 
CTTCAGCTCG AGAACCCACC GGTCGCTGTA CAGCTCGGGT CAGGATGGTC GTCTGTGAAG 
GAAGTAAACT GGTCAGCCTT GCAATCCCGG AGGTTTCATT CTGCTATAGC AAATGTGTTA 
CCAAAGAACA TTCATCTTCT CACCTTCAAA ACACCAGGCT GGAATTACAA CGTCTCTGCC 
AAAACCAGTC AATCTACGGG AGGATCCCCA CTCAATCTTA GCCGAATTCT GCTGCGGCTC 
CAGCACCTGT ACGAAGTAGG TGAACACCCC ATACTGTCCA GGAACGTCAC CGTAGATCTT 
CAAATGATTT TATCACCATT GGGGATGATA AAGACAGTGG ATGAGCGTAG CCTGACGGGT 
ATCTGGAACA TCACTCAGAT GAACAGGTGG AAGTGGAGAA GAGCAGATGA AGGTCGGTCT 
CAATCACCTG ACATGTCGAT CAGCAACACG ATTACCCACG CAGAACCAAC AACAACACTA 
ACCACAACCA CAGAACCAAC CACAACCACA GCGCCAGCGC CAACAACAAC TGCCACACCA 
CCTAGCTTTA CCTACACACT ACAACCACAG GAAATTAAGA CCTTCTTTGT CATCATGAAT 
GCACCGACCG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011673874.2
CDS	162..3416
Translation	MALLKRNFFIVITISVNFLIGSGVVTVQGLGVEREDEVDNAYSDGDTLNVYVIPHSHMDVGWVYTVKESMHAYAANVYTTVIAELGRNPARRFIAVEQEFFRLWWDGVATEKQKSQTRKFVSNGQLEFIIGGQVMHDEAVTELSAIIQQMTEGHAFIYETFGVRPRFGWHVDPFGATAATPTLFALMGFNAHLTSRINYDQKAAMMKAKGLQFVWQASPSLGSSQSMFTHVMDTYSYCAPMEHPPFSNRSAYMWNGVAKFPDPPADGIYPNMSDPVTTQNMGQYADFMVANIRKRAAWFKTPHLLWPWGCDKQFFNATDQYENMERLLVHINQQRSKYNIKIRQATLGQFFTALHNSDTTWDVKNSGDFIPYSTAREEAWTGFYTSRIELKGIARASQATLRAGETLFSLYTHLFNISRTINTTSALEKIKKLRWATAEVQHHDGVTGTEAPVVRDMYISHLRDGSQATISVLTDILNELTQVNARETEHHNERYNVLGLKSEISEGKSTLTYAVYNSLGWTVRRLVRIEVNSPNVTVLDARGQPVISQVNPPSNGSQLYQLYFFATIPPIGYQIFVLLFINENQRLLQSQCPTVRSYGAATEDAGGMDEGEGRGDSEGDEISIENECYKISFDRSTNALSSITNKQNGKTTTMSAGFLEYHVFHDVLYGQTSNNYVFRPYGPATVIGSVRTQLNVSTGVLVNETTQHFYGLFDVESRYSVTMRLINDASGHPDKLSCSHIEVDFKVGPLSMNKELVYRFSTLLKSNGNLYADVNAFQTMNRSRRHGATIAQNFYPMVSSAYIEDASNDERLMLLSERSHGVSSQSDGDIEVMLHRRVVNNEFETHNYNLTLEEPSVATSTFWLLLGNASTTSELRHRSWLQLENPPVAVQLGSGWSSVKEVNWSALQSRRFHSAIANVLPKNIHLLTFKTPGWNYNVSAKTSQSTGGSPLNLSRILLRLQHLYEVGEHPILSRNVTVDLQMILSPLGMIKTVDERSLTGIWNITQMNRWKWRRADEGRSQSPDMSISNTITHAEPTTTLTTTTEPTTTTAPAPTTTATPPSFTYTLQPQEIKTFFVIMNAPTE

Target ORF information:

RefSeq Version	XM_011675572.2
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus epididymis-specific alpha-mannosidase (LOC756277), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011675572.2

ORF Insert Sequence:

ATGGCGCTCC TGAAAAGGAA TTTCTTCATC GTAATAACCA TCTCTGTCAA CTTCTTGATT 
GGTTCCGGCG TGGTGACCGT CCAGGGACTT GGCGTCGAAA GAGAGGACGA AGTCGACAAT 
GCATATAGTG ATGGCGATAC CCTGAATGTT TATGTGATAC CCCATAGTCA CATGGACGTT 
GGATGGGTTT ACACAGTCAA GGAGAGTATG CACGCTTATG CCGCCAATGT CTACACCACA 
GTGATAGCTG AACTTGGTCG CAACCCTGCC CGCCGATTTA TTGCTGTAGA ACAGGAGTTC 
TTCAGGCTCT GGTGGGATGG CGTAGCCACA GAAAAACAAA AGAGTCAAAC TCGAAAGTTT 
GTTTCAAATG GACAGTTGGA GTTTATCATT GGTGGTCAGG TGATGCACGA TGAAGCAGTA 
ACTGAACTTA GCGCCATTAT ACAACAAATG ACAGAAGGAC ACGCCTTTAT CTACGAGACG 
TTTGGTGTTC GGCCCCGGTT CGGTTGGCAT GTTGATCCAT TCGGCGCAAC GGCGGCCACG 
CCCACTCTCT TTGCACTGAT GGGGTTTAAT GCCCATCTGA CGTCACGAAT AAATTATGAT 
CAGAAAGCCG CCATGATGAA GGCAAAGGGA CTCCAGTTTG TATGGCAAGC CTCCCCATCT 
CTGGGCTCAT CACAGAGCAT GTTCACACAC GTCATGGATA CGTATAGCTA CTGTGCTCCC 
ATGGAGCATC CGCCATTTTC AAACAGATCT GCATATATGT GGAATGGCGT AGCCAAGTTC 
CCAGATCCGC CAGCCGACGG TATCTACCCC AACATGAGCG ACCCAGTGAC GACACAAAAC 
ATGGGACAGT ATGCGGACTT CATGGTGGCC AACATTCGGA AACGAGCTGC GTGGTTCAAG 
ACCCCGCATC TACTCTGGCC ATGGGGATGT GATAAGCAGT TCTTCAATGC CACCGACCAG 
TATGAAAATA TGGAACGTCT TCTAGTGCAT ATAAACCAAC AGCGATCTAA ATACAATATC 
AAAATCCGAC AGGCGACACT CGGGCAGTTC TTCACCGCCC TTCACAACAG TGACACTACG 
TGGGACGTCA AGAACAGTGG AGATTTTATC CCTTATTCGA CCGCACGCGA GGAAGCTTGG 
ACCGGATTCT ACACCTCACG TATTGAGCTG AAGGGGATAG CCCGGGCGAG CCAGGCTACT 
CTAAGAGCTG GAGAGACCCT CTTCTCTCTG TATACCCATC TCTTTAACAT CTCAAGGACC 
ATTAATACCA CCAGCGCACT GGAGAAGATC AAGAAGCTAA GATGGGCTAC TGCCGAGGTG 
CAACATCACG ACGGCGTCAC GGGAACAGAA GCCCCTGTGG TGCGTGACAT GTATATCAGT 
CACCTACGTG ACGGCAGTCA AGCTACCATA AGTGTGCTGA CGGATATACT TAATGAATTG 
ACGCAAGTTA ACGCAAGGGA GACGGAACAC CACAACGAAA GATATAATGT ACTCGGGTTA 
AAATCAGAAA TTAGTGAAGG CAAGAGCACT CTAACCTACG CGGTCTACAA CTCTCTCGGA 
TGGACCGTTC GACGCCTAGT ACGAATTGAA GTTAACTCGC CCAATGTGAC GGTCCTTGAC 
GCACGGGGTC AACCCGTCAT TAGCCAGGTA AATCCGCCGT CGAATGGGAG TCAACTGTAC 
CAACTTTACT TCTTTGCAAC CATTCCACCT ATCGGCTACC AAATATTCGT CCTTTTATTT 
ATCAACGAAA ACCAACGTCT TTTACAAAGC CAGTGTCCCA CTGTGAGAAG TTACGGTGCC 
GCCACCGAGG ATGCGGGAGG CATGGATGAA GGAGAAGGGC GCGGTGATTC AGAAGGAGAT 
GAAATATCGA TCGAGAATGA ATGCTACAAA ATATCTTTTG ACCGTTCCAC GAATGCACTG 
TCGTCGATAA CAAACAAACA AAACGGCAAG ACCACTACAA TGAGCGCGGG ATTCCTGGAG 
TACCATGTCT TCCACGACGT ATTGTACGGC CAAACGTCCA ACAACTACGT ATTCAGACCC 
TACGGACCTG CCACGGTAAT CGGCAGCGTC AGGACTCAAC TTAATGTCAG CACGGGTGTC 
CTCGTAAACG AGACAACACA ACATTTCTAT GGGCTGTTCG ATGTGGAAAG TCGCTATTCA 
GTGACCATGC GATTGATCAA CGATGCAAGT GGACATCCTG ATAAGCTGTC GTGCAGCCAT 
ATTGAAGTGG ACTTCAAAGT TGGTCCTCTT TCTATGAACA AGGAACTCGT TTACAGGTTT 
TCTACATTGC TAAAGTCTAA TGGTAATCTG TACGCTGATG TTAATGCCTT CCAAACTATG 
AATAGGTCTA GGCGCCACGG TGCAACCATT GCACAGAACT TCTACCCAAT GGTAAGCTCG 
GCCTACATAG AGGATGCTTC CAACGATGAG AGATTGATGC TGCTCTCTGA GAGGAGCCAT 
GGAGTCAGCA GTCAGAGTGA TGGAGATATC GAGGTCATGT TGCATCGCAG AGTAGTCAAC 
AACGAGTTCG AGACCCACAA TTACAACCTC ACCTTGGAGG AACCTTCTGT AGCAACATCC 
ACGTTCTGGC TGTTGCTAGG CAACGCATCC ACGACATCGG AGCTTCGTCA TCGCAGCTGG 
CTTCAGCTCG AGAACCCACC GGTCGCTGTA CAGCTCGGGT CAGGATGGTC GTCTGTGAAG 
GAAGTAAACT GGTCAGCCTT GCAATCCCGG AGGTTTCATT CTGCTATAGC AAATGTGTTA 
CCAAAGAACA TTCATCTTCT CACCTTCAAA ACACCAGGCT GGAATTACAA CGTCTCTGCC 
AAAACCAGTC AATCTACGGG AGGATCCCCA CTCAATCTTA GCCGAATTCT GCTGCGGCTC 
CAGCACCTGT ACGAAGTAGG TGAACACCCC ATACTGTCCA GGAACGTCAC CGTAGATCTT 
CAAATGATTT TATCACCATT GGGGATGATA AAGACAGTGG ATGAGCGTAG CCTGACGGGT 
ATCTGGAACA TCACTCAGAT GAACAGGTGG AAGTGGAGAA GAGCAGATGA AGGTCGGTCT 
CAATCACCTG ACATGTCGAT CAGCAACACG ATTACCCACG CAGAACCAAC AACAACACTA 
ACCACAACCA CAGAACCAAC CACAACCACA GCGCCAGCGC CAACAACAAC TGCCACACCA 
CCTAGCTTTA CCTACACACT ACAACCACAG GAAATTAAGA CCTTCTTTGT CATCATGAAT 
GCACCGACCG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC756277 cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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