LOC100892953 cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100892953
Entrez Gene ID	100892953
Full Name	crooked neck-like protein 1
Gene Type	protein-coding
Organism	Strongylocentrotus purpuratus(purple sea urchin)

mRNA and Protein(s)

mRNA	Protein	Name
XM_778646.4	XP_783739.3	crooked neck-like protein 1 isoform X1
XM_011668137.1	XP_011666439.1	crooked neck-like protein 1 isoform X2
XM_030998405.1	XP_030854265.1	crooked neck-like protein 1
XM_011668138.1	XP_011666440.1	crooked neck-like protein 1 isoform X3

Pathways from BioSystems
Gene Ontology

The following LOC100892953 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100892953 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSd16006	XM_030998405.1 Latest version!	Strongylocentrotus purpuratus crooked neck-like protein 1 (LOC100892953), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OSd16006	XM_778646.4 Latest version!	Strongylocentrotus purpuratus crooked neck-like protein 1 (LOC100892953), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OSd32698	XM_011668137.1 Latest version!	Strongylocentrotus purpuratus crooked neck-like protein 1 (LOC100892953), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OSd32699	XM_011668138.1 Latest version!	Strongylocentrotus purpuratus crooked neck-like protein 1 (LOC100892953), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100892953 ( XM_778646.4 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_783739.3 Strongylocentrotus purpuratus crooked neck-like protein 1 (LOC100892953), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSd16006
Clone ID Related Accession (Same CDS sequence)	XM_778646.4 , XM_030998405.1
Accession Version	XM_778646.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1427040000000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	crooked neck-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011974404.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 24, 2015 this sequence version replaced XM_778646.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Strongylocentrotus purpuratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGT CCAGTAAAAA TAAAGTTCCC AAAGTTGCGA AGGTCAAAAA CAAGATGCCT 
GCAGAGGTGC AGATTACAGC TGAACAGATC CTCAGAGAGG CTAAGGAACG AGAACTTGAG 
TTGGTTCCTC CTCCTCCAAA GCAAAAGATC ACTGACCCAG ATGAACTTCA AGAATACAGA 
CTGAGAAAAA GAAAGGAATT TGAGGACAAT ATCCGCAAAA ACCGTTCCAT GGTGGGAAAT 
TGGATCAAGT ATGCCAAGTG GGAAGAGAGC CAGAATGAAA TTGCTCGAGC AAGATCCATT 
TGGGAAAGAG CCTTAGATGT GGAGCACAGG AACGTGACAA TCTGGTTGAA ATACGCAGAA 
ATGGAAATGA AACACAAACA GATCAACCAT TCTCGCAACA TCTGGGACAG AGCCATCACC 
ATCCTCCCTC GCACCAATCA GTTCTGGTAC AAGTACACCT ACATGGAGGA GCTTGTAGGG 
AACGTGGGTG GAGCCAGACA GGTGTTTGAA CGCTGGATGC AATGGGAACC TGAAGAACAG 
GCGTGGTTCT CCTACATTAA GATGGAGCTT AGATACAAGG AGACGGAGCG TGCAAGAGCT 
ATCTATGAGA GATTTGTCTA TGTTCATCCT GAAGTGAAAA ACTGGATCAA ATATGCCGGA 
TTTGAGGAAT CTCATAACTA CTTCAGTTTA GCCAGAGGAG TCTATGAGAG AGCTGTAGCA 
TTTTATGAAG ACCACATGGA TGAGAAACTC TACATAGCAT TTTCCAAGTT CGAAGAGAGA 
CAGAAAGAGC ATGAAAGAGC CAAGGTGATC TATAAATATG CTCTCGACAA CATGGATAAG 
GAGCATGCTC AAGAACTGTT CAAGAACTAC ACCATCCATG AGAAGAGATA TGGAGACAGA 
GCTGGCATAG AAGATGTCGT CATCAGCAAG AGGAGGTTCC AGTATGAAGA AGAGGTGAAA 
GCCAACCCTA ACAACTACGA TGCATGGTTT GACTACCTAC GTCTAATGGA GACGGATGGG 
GATATAGAGA CTGTGAGAGA TCTGTATGAG AGAGCTATAG CTAACATTCC ACCTGCTCAA 
GAAAAGAGAT TGTGGAGGAG ATATATGTAT CTATGGATCA ACTATGCTAC CTATGAAGAA 
TTAGAAGTCA GGGATATGGA AAAGACACGT GAGGTTTACA AGGCATGCCT GGACCTGATC 
CCCCATAAGA AGTTTACATT TGCCAAGATG TGGGTGCTGA TGGCTCAGTT TGAGGTCAGA 
CAGAAAGAGC TTCAGAAAGC AAGGAGAGTC ATGGGAACAG CGATTGGAAA GTGTCCAAAA 
GATAAGCTAT TCAAGTCTTA CATTGAGATG GAGCTTCAGC TGAGAGAGTT TGATCGTTGT 
AGAGTTCTCT ACGAGAAGTT CTTAGAATTC AACCCAGCAA ACTGCACCAC ATGGATGAAG 
TATGCTGAGC TGGAGACTAT TCTAGGAGAT ATCGACAGAT CAAGAGCTGT CTATGAACTT 
GCCATTAGTC AGCCTAGACT TGACATGCCA GAGGTTTTGT GGAAATCTTT CATTGACTTT 
GAAGTGGAGC AGGAAGAATG GGATAACTCT CGAGCTCTGT ATAGGAGACT ACTGGAGAGG 
ACACAACATG TTAAGGTCTG GATAAGTTTT GCTAAATGCG AGCTGTCGGT TGGATCAGAA 
GACTGCGTCT TGCGATCAAG ACAGGTCTAT GATGAAGCCA ACAAGGCATT GAAGCATGTG 
GAGGAGAAAG AAGAGAGACT GATGCTACTA GAGGCATGGC AGGAGTTTGA GAATGAATTT 
GGAGATGATG AGAGTGTAGA ACAGGTGCAA GAACAGATGC CTAACAAGGT CAAGAAGAGG 
AGAAAGATTC AAACAGAAGA TGGGTCTGAT GCAGGATGGG AAGAGTACTA CGACTACATC 
TTTCCTTCAG ATGAGACCAA CCAATCCAAT CTTAAGCTGC TTGCTATGGC TAAGATGTGG 
AAACAGAAGA TGGCTGAAAT CAATGACACC TCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_783739.3
CDS	96..2111
Translation	MASSSKNKVPKVAKVKNKMPAEVQITAEQILREAKERELELVPPPPKQKITDPDELQEYRLRKRKEFEDNIRKNRSMVGNWIKYAKWEESQNEIARARSIWERALDVEHRNVTIWLKYAEMEMKHKQINHSRNIWDRAITILPRTNQFWYKYTYMEELVGNVGGARQVFERWMQWEPEEQAWFSYIKMELRYKETERARAIYERFVYVHPEVKNWIKYAGFEESHNYFSLARGVYERAVAFYEDHMDEKLYIAFSKFEERQKEHERAKVIYKYALDNMDKEHAQELFKNYTIHEKRYGDRAGIEDVVISKRRFQYEEEVKANPNNYDAWFDYLRLMETDGDIETVRDLYERAIANIPPAQEKRLWRRYMYLWINYATYEELEVRDMEKTREVYKACLDLIPHKKFTFAKMWVLMAQFEVRQKELQKARRVMGTAIGKCPKDKLFKSYIEMELQLREFDRCRVLYEKFLEFNPANCTTWMKYAELETILGDIDRSRAVYELAISQPRLDMPEVLWKSFIDFEVEQEEWDNSRALYRRLLERTQHVKVWISFAKCELSVGSEDCVLRSRQVYDEANKALKHVEEKEERLMLLEAWQEFENEFGDDESVEQVQEQMPNKVKKRRKIQTEDGSDAGWEEYYDYIFPSDETNQSNLKLLAMAKMWKQKMAEINDTS

Target ORF information:

RefSeq Version	XM_778646.4
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus crooked neck-like protein 1 (LOC100892953), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_778646.4

ORF Insert Sequence:

ATGGCTTCGT CCAGTAAAAA TAAAGTTCCC AAAGTTGCGA AGGTCAAAAA CAAGATGCCT 
GCAGAGGTGC AGATTACAGC TGAACAGATC CTCAGAGAGG CTAAGGAACG AGAACTTGAG 
TTGGTTCCTC CTCCTCCAAA GCAAAAGATC ACTGACCCAG ATGAACTTCA AGAATACAGA 
CTGAGAAAAA GAAAGGAATT TGAGGACAAT ATCCGCAAAA ACCGTTCCAT GGTGGGAAAT 
TGGATCAAGT ATGCCAAGTG GGAAGAGAGC CAGAATGAAA TTGCTCGAGC AAGATCCATT 
TGGGAAAGAG CCTTAGATGT GGAGCACAGG AACGTGACAA TCTGGTTGAA ATACGCAGAA 
ATGGAAATGA AACACAAACA GATCAACCAT TCTCGCAACA TCTGGGACAG AGCCATCACC 
ATCCTCCCTC GCACCAATCA GTTCTGGTAC AAGTACACCT ACATGGAGGA GCTTGTAGGG 
AACGTGGGTG GAGCCAGACA GGTGTTTGAA CGCTGGATGC AATGGGAACC TGAAGAACAG 
GCGTGGTTCT CCTACATTAA GATGGAGCTT AGATACAAGG AGACGGAGCG TGCAAGAGCT 
ATCTATGAGA GATTTGTCTA TGTTCATCCT GAAGTGAAAA ACTGGATCAA ATATGCCGGA 
TTTGAGGAAT CTCATAACTA CTTCAGTTTA GCCAGAGGAG TCTATGAGAG AGCTGTAGCA 
TTTTATGAAG ACCACATGGA TGAGAAACTC TACATAGCAT TTTCCAAGTT CGAAGAGAGA 
CAGAAAGAGC ATGAAAGAGC CAAGGTGATC TATAAATATG CTCTCGACAA CATGGATAAG 
GAGCATGCTC AAGAACTGTT CAAGAACTAC ACCATCCATG AGAAGAGATA TGGAGACAGA 
GCTGGCATAG AAGATGTCGT CATCAGCAAG AGGAGGTTCC AGTATGAAGA AGAGGTGAAA 
GCCAACCCTA ACAACTACGA TGCATGGTTT GACTACCTAC GTCTAATGGA GACGGATGGG 
GATATAGAGA CTGTGAGAGA TCTGTATGAG AGAGCTATAG CTAACATTCC ACCTGCTCAA 
GAAAAGAGAT TGTGGAGGAG ATATATGTAT CTATGGATCA ACTATGCTAC CTATGAAGAA 
TTAGAAGTCA GGGATATGGA AAAGACACGT GAGGTTTACA AGGCATGCCT GGACCTGATC 
CCCCATAAGA AGTTTACATT TGCCAAGATG TGGGTGCTGA TGGCTCAGTT TGAGGTCAGA 
CAGAAAGAGC TTCAGAAAGC AAGGAGAGTC ATGGGAACAG CGATTGGAAA GTGTCCAAAA 
GATAAGCTAT TCAAGTCTTA CATTGAGATG GAGCTTCAGC TGAGAGAGTT TGATCGTTGT 
AGAGTTCTCT ACGAGAAGTT CTTAGAATTC AACCCAGCAA ACTGCACCAC ATGGATGAAG 
TATGCTGAGC TGGAGACTAT TCTAGGAGAT ATCGACAGAT CAAGAGCTGT CTATGAACTT 
GCCATTAGTC AGCCTAGACT TGACATGCCA GAGGTTTTGT GGAAATCTTT CATTGACTTT 
GAAGTGGAGC AGGAAGAATG GGATAACTCT CGAGCTCTGT ATAGGAGACT ACTGGAGAGG 
ACACAACATG TTAAGGTCTG GATAAGTTTT GCTAAATGCG AGCTGTCGGT TGGATCAGAA 
GACTGCGTCT TGCGATCAAG ACAGGTCTAT GATGAAGCCA ACAAGGCATT GAAGCATGTG 
GAGGAGAAAG AAGAGAGACT GATGCTACTA GAGGCATGGC AGGAGTTTGA GAATGAATTT 
GGAGATGATG AGAGTGTAGA ACAGGTGCAA GAACAGATGC CTAACAAGGT CAAGAAGAGG 
AGAAAGATTC AAACAGAAGA TGGGTCTGAT GCAGGATGGG AAGAGTACTA CGACTACATC 
TTTCCTTCAG ATGAGACCAA CCAATCCAAT CTTAAGCTGC TTGCTATGGC TAAGATGTGG 
AAACAGAAGA TGGCTGAAAT CAATGACACC TCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100892953 ( XM_030998405.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_030854265.1 Strongylocentrotus purpuratus crooked neck-like protein 1 (LOC100892953), mRNA.

Price & Availability↑

CloneID	OSd16006
Clone ID Related Accession (Same CDS sequence)	XM_778646.4 , XM_030998405.1
Accession Version	XM_030998405.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569427200000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	crooked neck-like protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022145611.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Strongylocentrotus purpuratus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGT CCAGTAAAAA TAAAGTTCCC AAAGTTGCGA AGGTCAAAAA CAAGATGCCT 
GCAGAGGTGC AGATTACAGC TGAACAGATC CTCAGAGAGG CTAAGGAACG AGAACTTGAG 
TTGGTTCCTC CTCCTCCAAA GCAAAAGATC ACTGACCCAG ATGAACTTCA AGAATACAGA 
CTGAGAAAAA GAAAGGAATT TGAGGACAAT ATCCGCAAAA ACCGTTCCAT GGTGGGAAAT 
TGGATCAAGT ATGCCAAGTG GGAAGAGAGC CAGAATGAAA TTGCTCGAGC AAGATCCATT 
TGGGAAAGAG CCTTAGATGT GGAGCACAGG AACGTGACAA TCTGGTTGAA ATACGCAGAA 
ATGGAAATGA AACACAAACA GATCAACCAT TCTCGCAACA TCTGGGACAG AGCCATCACC 
ATCCTCCCTC GCACCAATCA GTTCTGGTAC AAGTACACCT ACATGGAGGA GCTTGTAGGG 
AACGTGGGTG GAGCCAGACA GGTGTTTGAA CGCTGGATGC AATGGGAACC TGAAGAACAG 
GCGTGGTTCT CCTACATTAA GATGGAGCTT AGATACAAGG AGACGGAGCG TGCAAGAGCT 
ATCTATGAGA GATTTGTCTA TGTTCATCCT GAAGTGAAAA ACTGGATCAA ATATGCCGGA 
TTTGAGGAAT CTCATAACTA CTTCAGTTTA GCCAGAGGAG TCTATGAGAG AGCTGTAGCA 
TTTTATGAAG ACCACATGGA TGAGAAACTC TACATAGCAT TTTCCAAGTT CGAAGAGAGA 
CAGAAAGAGC ATGAAAGAGC CAAGGTGATC TATAAATATG CTCTCGACAA CATGGATAAG 
GAGCATGCTC AAGAACTGTT CAAGAACTAC ACCATCCATG AGAAGAGATA TGGAGACAGA 
GCTGGCATAG AAGATGTCGT CATCAGCAAG AGGAGGTTCC AGTATGAAGA AGAGGTGAAA 
GCCAACCCTA ACAACTACGA TGCATGGTTT GACTACCTAC GTCTAATGGA GACGGATGGG 
GATATAGAGA CTGTGAGAGA TCTGTATGAG AGAGCTATAG CTAACATTCC ACCTGCTCAA 
GAAAAGAGAT TGTGGAGGAG ATATATGTAT CTATGGATCA ACTATGCTAC CTATGAAGAA 
TTAGAAGTCA GGGATATGGA AAAGACACGT GAGGTTTACA AGGCATGCCT GGACCTGATC 
CCCCATAAGA AGTTTACATT TGCCAAGATG TGGGTGCTGA TGGCTCAGTT TGAGGTCAGA 
CAGAAAGAGC TTCAGAAAGC AAGGAGAGTC ATGGGAACAG CGATTGGAAA GTGTCCAAAA 
GATAAGCTAT TCAAGTCTTA CATTGAGATG GAGCTTCAGC TGAGAGAGTT TGATCGTTGT 
AGAGTTCTCT ACGAGAAGTT CTTAGAATTC AACCCAGCAA ACTGCACCAC ATGGATGAAG 
TATGCTGAGC TGGAGACTAT TCTAGGAGAT ATCGACAGAT CAAGAGCTGT CTATGAACTT 
GCCATTAGTC AGCCTAGACT TGACATGCCA GAGGTTTTGT GGAAATCTTT CATTGACTTT 
GAAGTGGAGC AGGAAGAATG GGATAACTCT CGAGCTCTGT ATAGGAGACT ACTGGAGAGG 
ACACAACATG TTAAGGTCTG GATAAGTTTT GCTAAATGCG AGCTGTCGGT TGGATCAGAA 
GACTGCGTCT TGCGATCAAG ACAGGTCTAT GATGAAGCCA ACAAGGCATT GAAGCATGTG 
GAGGAGAAAG AAGAGAGACT GATGCTACTA GAGGCATGGC AGGAGTTTGA GAATGAATTT 
GGAGATGATG AGAGTGTAGA ACAGGTGCAA GAACAGATGC CTAACAAGGT CAAGAAGAGG 
AGAAAGATTC AAACAGAAGA TGGGTCTGAT GCAGGATGGG AAGAGTACTA CGACTACATC 
TTTCCTTCAG ATGAGACCAA CCAATCCAAT CTTAAGCTGC TTGCTATGGC TAAGATGTGG 
AAACAGAAGA TGGCTGAAAT CAATGACACC TCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030854265.1
CDS	109..2124
Translation	MASSSKNKVPKVAKVKNKMPAEVQITAEQILREAKERELELVPPPPKQKITDPDELQEYRLRKRKEFEDNIRKNRSMVGNWIKYAKWEESQNEIARARSIWERALDVEHRNVTIWLKYAEMEMKHKQINHSRNIWDRAITILPRTNQFWYKYTYMEELVGNVGGARQVFERWMQWEPEEQAWFSYIKMELRYKETERARAIYERFVYVHPEVKNWIKYAGFEESHNYFSLARGVYERAVAFYEDHMDEKLYIAFSKFEERQKEHERAKVIYKYALDNMDKEHAQELFKNYTIHEKRYGDRAGIEDVVISKRRFQYEEEVKANPNNYDAWFDYLRLMETDGDIETVRDLYERAIANIPPAQEKRLWRRYMYLWINYATYEELEVRDMEKTREVYKACLDLIPHKKFTFAKMWVLMAQFEVRQKELQKARRVMGTAIGKCPKDKLFKSYIEMELQLREFDRCRVLYEKFLEFNPANCTTWMKYAELETILGDIDRSRAVYELAISQPRLDMPEVLWKSFIDFEVEQEEWDNSRALYRRLLERTQHVKVWISFAKCELSVGSEDCVLRSRQVYDEANKALKHVEEKEERLMLLEAWQEFENEFGDDESVEQVQEQMPNKVKKRRKIQTEDGSDAGWEEYYDYIFPSDETNQSNLKLLAMAKMWKQKMAEINDTS

Target ORF information:

RefSeq Version	XM_030998405.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus crooked neck-like protein 1 (LOC100892953), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030998405.1

ORF Insert Sequence:

ATGGCTTCGT CCAGTAAAAA TAAAGTTCCC AAAGTTGCGA AGGTCAAAAA CAAGATGCCT 
GCAGAGGTGC AGATTACAGC TGAACAGATC CTCAGAGAGG CTAAGGAACG AGAACTTGAG 
TTGGTTCCTC CTCCTCCAAA GCAAAAGATC ACTGACCCAG ATGAACTTCA AGAATACAGA 
CTGAGAAAAA GAAAGGAATT TGAGGACAAT ATCCGCAAAA ACCGTTCCAT GGTGGGAAAT 
TGGATCAAGT ATGCCAAGTG GGAAGAGAGC CAGAATGAAA TTGCTCGAGC AAGATCCATT 
TGGGAAAGAG CCTTAGATGT GGAGCACAGG AACGTGACAA TCTGGTTGAA ATACGCAGAA 
ATGGAAATGA AACACAAACA GATCAACCAT TCTCGCAACA TCTGGGACAG AGCCATCACC 
ATCCTCCCTC GCACCAATCA GTTCTGGTAC AAGTACACCT ACATGGAGGA GCTTGTAGGG 
AACGTGGGTG GAGCCAGACA GGTGTTTGAA CGCTGGATGC AATGGGAACC TGAAGAACAG 
GCGTGGTTCT CCTACATTAA GATGGAGCTT AGATACAAGG AGACGGAGCG TGCAAGAGCT 
ATCTATGAGA GATTTGTCTA TGTTCATCCT GAAGTGAAAA ACTGGATCAA ATATGCCGGA 
TTTGAGGAAT CTCATAACTA CTTCAGTTTA GCCAGAGGAG TCTATGAGAG AGCTGTAGCA 
TTTTATGAAG ACCACATGGA TGAGAAACTC TACATAGCAT TTTCCAAGTT CGAAGAGAGA 
CAGAAAGAGC ATGAAAGAGC CAAGGTGATC TATAAATATG CTCTCGACAA CATGGATAAG 
GAGCATGCTC AAGAACTGTT CAAGAACTAC ACCATCCATG AGAAGAGATA TGGAGACAGA 
GCTGGCATAG AAGATGTCGT CATCAGCAAG AGGAGGTTCC AGTATGAAGA AGAGGTGAAA 
GCCAACCCTA ACAACTACGA TGCATGGTTT GACTACCTAC GTCTAATGGA GACGGATGGG 
GATATAGAGA CTGTGAGAGA TCTGTATGAG AGAGCTATAG CTAACATTCC ACCTGCTCAA 
GAAAAGAGAT TGTGGAGGAG ATATATGTAT CTATGGATCA ACTATGCTAC CTATGAAGAA 
TTAGAAGTCA GGGATATGGA AAAGACACGT GAGGTTTACA AGGCATGCCT GGACCTGATC 
CCCCATAAGA AGTTTACATT TGCCAAGATG TGGGTGCTGA TGGCTCAGTT TGAGGTCAGA 
CAGAAAGAGC TTCAGAAAGC AAGGAGAGTC ATGGGAACAG CGATTGGAAA GTGTCCAAAA 
GATAAGCTAT TCAAGTCTTA CATTGAGATG GAGCTTCAGC TGAGAGAGTT TGATCGTTGT 
AGAGTTCTCT ACGAGAAGTT CTTAGAATTC AACCCAGCAA ACTGCACCAC ATGGATGAAG 
TATGCTGAGC TGGAGACTAT TCTAGGAGAT ATCGACAGAT CAAGAGCTGT CTATGAACTT 
GCCATTAGTC AGCCTAGACT TGACATGCCA GAGGTTTTGT GGAAATCTTT CATTGACTTT 
GAAGTGGAGC AGGAAGAATG GGATAACTCT CGAGCTCTGT ATAGGAGACT ACTGGAGAGG 
ACACAACATG TTAAGGTCTG GATAAGTTTT GCTAAATGCG AGCTGTCGGT TGGATCAGAA 
GACTGCGTCT TGCGATCAAG ACAGGTCTAT GATGAAGCCA ACAAGGCATT GAAGCATGTG 
GAGGAGAAAG AAGAGAGACT GATGCTACTA GAGGCATGGC AGGAGTTTGA GAATGAATTT 
GGAGATGATG AGAGTGTAGA ACAGGTGCAA GAACAGATGC CTAACAAGGT CAAGAAGAGG 
AGAAAGATTC AAACAGAAGA TGGGTCTGAT GCAGGATGGG AAGAGTACTA CGACTACATC 
TTTCCTTCAG ATGAGACCAA CCAATCCAAT CTTAAGCTGC TTGCTATGGC TAAGATGTGG 
AAACAGAAGA TGGCTGAAAT CAATGACACC TCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100892953 ( XM_011668137.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_011666439.1 Strongylocentrotus purpuratus crooked neck-like protein 1 (LOC100892953), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSd32698
Clone ID Related Accession (Same CDS sequence)	XM_011668137.1
Accession Version	XM_011668137.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1427040000000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	crooked neck-like protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011974404.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Strongylocentrotus purpuratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGT CTAGTAAAAA TAAAGTTCCC AAAGTCGCGA AGGTCAAAAA CAAGATGCCT 
GCAGAGGTGC AGATTACTGC TGAACAGATC CTCAGAGAGG CTAAGGAACG AGAACTTGAG 
TTGGTTCCTC CTCCTCCAAA GCAAAAGATC ACTGACCCAG ATGAACTTCA GGAATACAGA 
CTGAGAAAAA GAAAAGAATT TGAGGACAAT ATTCGCAAGA ACCGTTCCAT GGTGGGAAAT 
TGGATCAAGT ATGCCAAGTG GGAAGAGAGC CAGAATGAAA TTGCTCGAGC AAGATCCATT 
TGGGAAAGAG CCTTAGATGT GGAGCACAGG AATGTGACAA TCTGGTTGAA ATACGCAGAA 
ATGGAAATGA AACACAAACA GATCAACCAT TCTCGCAACA TCTGGGACAG AGCCATAACC 
ATCCTCCCTC GCACCAATCA GTTCTGGTAC AAGTACACCT ACATGGAGGA GCTTGTAGGT 
AACGTGGGTG GAGCCAGACA GGTGTTTGAA CGGTGGATGC AATGGGAACC TGAAGAACAG 
GCGTGGTTCT CCTACATCAA GATGGAGCTT AGATACAAGG AGACGGAGCG TGCAAGAGCT 
ATCTATGAGA GATTTGTCTA TGTGCATCCT GAAGTGAAAA ACTGGATCAA ATATGCAGGA 
TTTGAGGAAT CTCATAACTA CTTCAGTCTA GCCAGGGGAG TCTATGAGAG AGCTGTAGCA 
TTTTATGAAG ATCACATGGA TGAGAAACTC TACATAGCAT TTTCCAAGTT TGAAGAGAGA 
CAGAAAGAGC ATGAAAGAGC CAAGGTGATC TATAAATATG CTCTCGACAA CATGGATAAG 
GAGCATGCTC AAGAACTGTT CAAGAACTAC ACCATCCATG AGAAGAGATA TGGAGACAGA 
GCTGGCATAG AAGATGTCGT CATCAGCAAG AGGAGGTTCC AGTATGAAGA AGAGGTGAAA 
GCCAACCCTA ACAACTACGA TGCATGGTTT GACTACCTAC GTCTAATGGA GACGGATGGG 
GATATAGAGA CTGTGAGAGA TCTGTATGAG AGAGCTATAG CTAACATTCC ACCTGCTCAA 
GAAAAGAGAT TGTGGAGGAG ATATATGTAT CTATGGATCA ACTATGCTAC CTATGAAGAA 
TTAGAAGTCA GGGATATGGA AAAGACACGT GAGGTTTACA AGGCATGCCT GGACCTGATC 
CCCCATAAGA AGTTTACATT TGCCAAGATG TGGGTGCTGA TGGCTCAGTT TGAGGTCAGA 
CAGAAAGAGC TTCAGAAAGC AAGGAGAGTC ATGGGAACAG CGATTGGAAA GTGTCCAAAA 
GATAAGCTAT TCAAGTCTTA CATTGAGATG GAGCTTCAGC TGAGAGAGTT TGATCGTTGT 
AGAGTTCTCT ACGAGAAGTT CTTAGAATTC AACCCAGCAA ACTGCACCAC ATGGATGAAG 
TATGCTGAGC TGGAGACTAT TCTAGGAGAT ATCGACAGAT CAAGAGCTGT CTATGAACTT 
GCCATTAGTC AGCCTAGACT TGACATGCCA GAGGTTTTGT GGAAATCTTT CATTGACTTT 
GAAGTGGAGC AGGAAGAATG GGATAACTCT CGAGCTCTGT ATAGGAGACT ACTGGAGAGG 
ACACAACATG TTAAGGTCTG GATAAGTTTT GCTAAATGCG AGCTGTCGGT TGGATCAGAA 
GACTGCGTCT TGCGATCAAG ACAGGTCTAT GATGAAGCCA ACAAGGCATT GAAGCATGTG 
GAGGAGAAAG AAGAGAGACT GATGCTACTA GAGGCATGGC AGGAGTTTGA GAATGAATTT 
GGAGATGATG AGAGTGTAGA ACAGGTGCAA GAACAGATGC CTAACAAGGT CAAGAAGAGG 
AGAAAGATTC AAACAGAAGA TGGGTCTGAT GCAGGATGGG AAGAGTACTA CGACTACATC 
TTTCCTTCAG ATGAGACCAA CCAATCCAAT CTTAAGCTGC TTGCTATGGC TAAGATGTGG 
AAACAGAAGA TGGCTGAAAT CAATGACACC TCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011666439.1
CDS	84..2099
Translation	MASSSKNKVPKVAKVKNKMPAEVQITAEQILREAKERELELVPPPPKQKITDPDELQEYRLRKRKEFEDNIRKNRSMVGNWIKYAKWEESQNEIARARSIWERALDVEHRNVTIWLKYAEMEMKHKQINHSRNIWDRAITILPRTNQFWYKYTYMEELVGNVGGARQVFERWMQWEPEEQAWFSYIKMELRYKETERARAIYERFVYVHPEVKNWIKYAGFEESHNYFSLARGVYERAVAFYEDHMDEKLYIAFSKFEERQKEHERAKVIYKYALDNMDKEHAQELFKNYTIHEKRYGDRAGIEDVVISKRRFQYEEEVKANPNNYDAWFDYLRLMETDGDIETVRDLYERAIANIPPAQEKRLWRRYMYLWINYATYEELEVRDMEKTREVYKACLDLIPHKKFTFAKMWVLMAQFEVRQKELQKARRVMGTAIGKCPKDKLFKSYIEMELQLREFDRCRVLYEKFLEFNPANCTTWMKYAELETILGDIDRSRAVYELAISQPRLDMPEVLWKSFIDFEVEQEEWDNSRALYRRLLERTQHVKVWISFAKCELSVGSEDCVLRSRQVYDEANKALKHVEEKEERLMLLEAWQEFENEFGDDESVEQVQEQMPNKVKKRRKIQTEDGSDAGWEEYYDYIFPSDETNQSNLKLLAMAKMWKQKMAEINDTS

Target ORF information:

RefSeq Version	XM_011668137.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus crooked neck-like protein 1 (LOC100892953), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011668137.1

ORF Insert Sequence:

ATGGCTTCGT CTAGTAAAAA TAAAGTTCCC AAAGTCGCGA AGGTCAAAAA CAAGATGCCT 
GCAGAGGTGC AGATTACTGC TGAACAGATC CTCAGAGAGG CTAAGGAACG AGAACTTGAG 
TTGGTTCCTC CTCCTCCAAA GCAAAAGATC ACTGACCCAG ATGAACTTCA GGAATACAGA 
CTGAGAAAAA GAAAAGAATT TGAGGACAAT ATTCGCAAGA ACCGTTCCAT GGTGGGAAAT 
TGGATCAAGT ATGCCAAGTG GGAAGAGAGC CAGAATGAAA TTGCTCGAGC AAGATCCATT 
TGGGAAAGAG CCTTAGATGT GGAGCACAGG AATGTGACAA TCTGGTTGAA ATACGCAGAA 
ATGGAAATGA AACACAAACA GATCAACCAT TCTCGCAACA TCTGGGACAG AGCCATAACC 
ATCCTCCCTC GCACCAATCA GTTCTGGTAC AAGTACACCT ACATGGAGGA GCTTGTAGGT 
AACGTGGGTG GAGCCAGACA GGTGTTTGAA CGGTGGATGC AATGGGAACC TGAAGAACAG 
GCGTGGTTCT CCTACATCAA GATGGAGCTT AGATACAAGG AGACGGAGCG TGCAAGAGCT 
ATCTATGAGA GATTTGTCTA TGTGCATCCT GAAGTGAAAA ACTGGATCAA ATATGCAGGA 
TTTGAGGAAT CTCATAACTA CTTCAGTCTA GCCAGGGGAG TCTATGAGAG AGCTGTAGCA 
TTTTATGAAG ATCACATGGA TGAGAAACTC TACATAGCAT TTTCCAAGTT TGAAGAGAGA 
CAGAAAGAGC ATGAAAGAGC CAAGGTGATC TATAAATATG CTCTCGACAA CATGGATAAG 
GAGCATGCTC AAGAACTGTT CAAGAACTAC ACCATCCATG AGAAGAGATA TGGAGACAGA 
GCTGGCATAG AAGATGTCGT CATCAGCAAG AGGAGGTTCC AGTATGAAGA AGAGGTGAAA 
GCCAACCCTA ACAACTACGA TGCATGGTTT GACTACCTAC GTCTAATGGA GACGGATGGG 
GATATAGAGA CTGTGAGAGA TCTGTATGAG AGAGCTATAG CTAACATTCC ACCTGCTCAA 
GAAAAGAGAT TGTGGAGGAG ATATATGTAT CTATGGATCA ACTATGCTAC CTATGAAGAA 
TTAGAAGTCA GGGATATGGA AAAGACACGT GAGGTTTACA AGGCATGCCT GGACCTGATC 
CCCCATAAGA AGTTTACATT TGCCAAGATG TGGGTGCTGA TGGCTCAGTT TGAGGTCAGA 
CAGAAAGAGC TTCAGAAAGC AAGGAGAGTC ATGGGAACAG CGATTGGAAA GTGTCCAAAA 
GATAAGCTAT TCAAGTCTTA CATTGAGATG GAGCTTCAGC TGAGAGAGTT TGATCGTTGT 
AGAGTTCTCT ACGAGAAGTT CTTAGAATTC AACCCAGCAA ACTGCACCAC ATGGATGAAG 
TATGCTGAGC TGGAGACTAT TCTAGGAGAT ATCGACAGAT CAAGAGCTGT CTATGAACTT 
GCCATTAGTC AGCCTAGACT TGACATGCCA GAGGTTTTGT GGAAATCTTT CATTGACTTT 
GAAGTGGAGC AGGAAGAATG GGATAACTCT CGAGCTCTGT ATAGGAGACT ACTGGAGAGG 
ACACAACATG TTAAGGTCTG GATAAGTTTT GCTAAATGCG AGCTGTCGGT TGGATCAGAA 
GACTGCGTCT TGCGATCAAG ACAGGTCTAT GATGAAGCCA ACAAGGCATT GAAGCATGTG 
GAGGAGAAAG AAGAGAGACT GATGCTACTA GAGGCATGGC AGGAGTTTGA GAATGAATTT 
GGAGATGATG AGAGTGTAGA ACAGGTGCAA GAACAGATGC CTAACAAGGT CAAGAAGAGG 
AGAAAGATTC AAACAGAAGA TGGGTCTGAT GCAGGATGGG AAGAGTACTA CGACTACATC 
TTTCCTTCAG ATGAGACCAA CCAATCCAAT CTTAAGCTGC TTGCTATGGC TAAGATGTGG 
AAACAGAAGA TGGCTGAAAT CAATGACACC TCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100892953 ( XM_011668138.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_011666440.1 Strongylocentrotus purpuratus crooked neck-like protein 1 (LOC100892953), transcript variant X3, mRNA.

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CloneID	OSd32699
Clone ID Related Accession (Same CDS sequence)	XM_011668138.1
Accession Version	XM_011668138.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1427040000000
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	crooked neck-like protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011974404.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Strongylocentrotus purpuratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGT CTAGTAAAAA TAAAGTTCCC AAAGTCGCGA AGGTCAAAAA CAAGATGCCT 
GCAGAGGTGC AGATTACTGC TGAACAGATC CTCAGAGAGG CTAAGGAACG AGAACTTGAG 
TTGGTTCCTC CTCCTCCAAA GCAAAAGATC ACTGACCCAG ATGAACTTCA GGAATACAGA 
CTGAGAAAAA GAAAAGAATT TGAGGACAAT ATTCGCAAGA ACCGTTCCAT GGTGGGAAAT 
TGGATCAAGT ATGCCAAGTG GGAAGAGAGC CAGAATGAAA TTGCTCGAGC AAGATCCATT 
TGGGAAAGAG CCTTAGATGT GGAGCACAGG AATGTGACAA TCTGGTTGAA ATACGCAGAA 
ATGGAAATGA AACACAAACA GATCAACCAT TCTCGCAACA TCTGGGACAG AGCCATAACC 
ATCCTCCCTC GCACCAATCA GTTCTGGTAC AAGTACACCT ACATGGAGGA GCTTGTAGGT 
AACGTGGGTG GAGCCAGACA GGTGTTTGAA CGGTGGATGC AATGGGAACC TGAAGAACAG 
GCGTGGTTCT CCTACATCAA GATGGAGCTT AGATACAAGG AGACGGAGCG TGCAAGAGCT 
ATCTATGAGA GATTTGTCTA TGTGCATCCT GAAGTGAAAA ACTGGATCAA ATATGCAGGA 
TTTGAGGAAT CTCATAACTA CTTCAGTCTA GCCAGGGGAG TCTATGAGAG AGCTGTAGCA 
TTTTATGAAG ATCACATGGA TGAGAAACTC TACATAGCAT TTTCCAAGTT TGAAGAGAGA 
CAGAAAGAGC ATGAAAGAGC CAAGGTGATC TACAAGTATG CTCTGGACAA CATGGATAAG 
GAGCATGCTC AAGAACTGTT CAAGAACTAC ACCATCCATG AGAAGAGATA TGGAGACAGA 
GCTGGCATAG AAGATGTCGT CATCAGCAAG AGGAGGTTCC AGTATGAAGA AGAGGTGAAA 
GCCAACCCTA ACAACTACGA TGCATGGTTT GACTACCTAC GTCTAATGGA GACGGATGGA 
GATATAGAGA CTGTGAGAGA TCTGTATGAG AGAGCTATTG CTAACATTCC ACCTGCTCAA 
GAAAAGAGAT TGTGGAGGAG ATATATGTAT CTATGGATCA ACTATGCTAC CTATGAAGAA 
TTAGAAGTCA GGGATATGGA GAAGACACGT GAGGTTTACA AGGCATGCCT GGACCTTATC 
CCTCATAAGA AGTTTACCTT TGCCAAGATG TGGGTGCTGA TGGCTCAGTT TGAGGTCAGA 
CAGAAAGAGC TTCAGAAAGC AAGGAGAGTC ATGGGAACAG CGATTGGAAA GTGTCCAAAA 
GATAAGCTAT TCAAGTCTTA CATTGAGATG GAGCTTCAGC TGAGAGAGTT TGATCGTTGT 
AGAGTTCTCT ATGAGAAGTT CTTAGAATTC AACCCAGCAA ACTGCACAAC ATGGATGAAG 
TATGCAGAGC TTGAGACTAT TCTTGGAGAT ATCGACAGAT CAAGAGCTGT CTATGAACTT 
GCCATCAGTC AGCCTAGACT TGACATGCCA GAGGTTTTGT GGAAATCTTT CATTGACTTT 
GAGGTGGAGC AAGAAGAATG GGATAACTCG AGAGCTCTGT ACAGGAGACT ACTGGAGAGG 
ACACAACATG TTAAGGTCTG GATAAGTTTT GCTAAATGCG AGCTGTCGGT TGGATCAGAT 
GACTGTGTCT TAAGATCAAG ACAGGTCTAT GATGAAGCCA ACAAGGCGTT GAAGCATGTG 
GAGGAGAAAG AAGAGAGACT GATGCTACTA GAGGCATGGC AGGAGTTTGA GAATGAATTT 
GGAGACGATG AGAGTGTAGA ACAGGTTCAA GAACAGATGC CTAACAAGGT CAAGAAGAGG 
AGAAAGATTC AGACAGAAGA TGGGTCTGAT GCAGGATGGG AAGAGTACTA CGACTACATC 
TTTCCTTCAG ATGAGACCAA CCAATCCAAT CTTAAGCTGC TTGCTATGGC TAAGATGTGG 
AAACAGAAGA TGGCTGAAAT CAATGACACC TCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011666440.1
CDS	87..2102
Translation	MASSSKNKVPKVAKVKNKMPAEVQITAEQILREAKERELELVPPPPKQKITDPDELQEYRLRKRKEFEDNIRKNRSMVGNWIKYAKWEESQNEIARARSIWERALDVEHRNVTIWLKYAEMEMKHKQINHSRNIWDRAITILPRTNQFWYKYTYMEELVGNVGGARQVFERWMQWEPEEQAWFSYIKMELRYKETERARAIYERFVYVHPEVKNWIKYAGFEESHNYFSLARGVYERAVAFYEDHMDEKLYIAFSKFEERQKEHERAKVIYKYALDNMDKEHAQELFKNYTIHEKRYGDRAGIEDVVISKRRFQYEEEVKANPNNYDAWFDYLRLMETDGDIETVRDLYERAIANIPPAQEKRLWRRYMYLWINYATYEELEVRDMEKTREVYKACLDLIPHKKFTFAKMWVLMAQFEVRQKELQKARRVMGTAIGKCPKDKLFKSYIEMELQLREFDRCRVLYEKFLEFNPANCTTWMKYAELETILGDIDRSRAVYELAISQPRLDMPEVLWKSFIDFEVEQEEWDNSRALYRRLLERTQHVKVWISFAKCELSVGSDDCVLRSRQVYDEANKALKHVEEKEERLMLLEAWQEFENEFGDDESVEQVQEQMPNKVKKRRKIQTEDGSDAGWEEYYDYIFPSDETNQSNLKLLAMAKMWKQKMAEINDTS

Target ORF information:

RefSeq Version	XM_011668138.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus crooked neck-like protein 1 (LOC100892953), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011668138.1

ORF Insert Sequence:

ATGGCTTCGT CTAGTAAAAA TAAAGTTCCC AAAGTCGCGA AGGTCAAAAA CAAGATGCCT 
GCAGAGGTGC AGATTACTGC TGAACAGATC CTCAGAGAGG CTAAGGAACG AGAACTTGAG 
TTGGTTCCTC CTCCTCCAAA GCAAAAGATC ACTGACCCAG ATGAACTTCA GGAATACAGA 
CTGAGAAAAA GAAAAGAATT TGAGGACAAT ATTCGCAAGA ACCGTTCCAT GGTGGGAAAT 
TGGATCAAGT ATGCCAAGTG GGAAGAGAGC CAGAATGAAA TTGCTCGAGC AAGATCCATT 
TGGGAAAGAG CCTTAGATGT GGAGCACAGG AATGTGACAA TCTGGTTGAA ATACGCAGAA 
ATGGAAATGA AACACAAACA GATCAACCAT TCTCGCAACA TCTGGGACAG AGCCATAACC 
ATCCTCCCTC GCACCAATCA GTTCTGGTAC AAGTACACCT ACATGGAGGA GCTTGTAGGT 
AACGTGGGTG GAGCCAGACA GGTGTTTGAA CGGTGGATGC AATGGGAACC TGAAGAACAG 
GCGTGGTTCT CCTACATCAA GATGGAGCTT AGATACAAGG AGACGGAGCG TGCAAGAGCT 
ATCTATGAGA GATTTGTCTA TGTGCATCCT GAAGTGAAAA ACTGGATCAA ATATGCAGGA 
TTTGAGGAAT CTCATAACTA CTTCAGTCTA GCCAGGGGAG TCTATGAGAG AGCTGTAGCA 
TTTTATGAAG ATCACATGGA TGAGAAACTC TACATAGCAT TTTCCAAGTT TGAAGAGAGA 
CAGAAAGAGC ATGAAAGAGC CAAGGTGATC TACAAGTATG CTCTGGACAA CATGGATAAG 
GAGCATGCTC AAGAACTGTT CAAGAACTAC ACCATCCATG AGAAGAGATA TGGAGACAGA 
GCTGGCATAG AAGATGTCGT CATCAGCAAG AGGAGGTTCC AGTATGAAGA AGAGGTGAAA 
GCCAACCCTA ACAACTACGA TGCATGGTTT GACTACCTAC GTCTAATGGA GACGGATGGA 
GATATAGAGA CTGTGAGAGA TCTGTATGAG AGAGCTATTG CTAACATTCC ACCTGCTCAA 
GAAAAGAGAT TGTGGAGGAG ATATATGTAT CTATGGATCA ACTATGCTAC CTATGAAGAA 
TTAGAAGTCA GGGATATGGA GAAGACACGT GAGGTTTACA AGGCATGCCT GGACCTTATC 
CCTCATAAGA AGTTTACCTT TGCCAAGATG TGGGTGCTGA TGGCTCAGTT TGAGGTCAGA 
CAGAAAGAGC TTCAGAAAGC AAGGAGAGTC ATGGGAACAG CGATTGGAAA GTGTCCAAAA 
GATAAGCTAT TCAAGTCTTA CATTGAGATG GAGCTTCAGC TGAGAGAGTT TGATCGTTGT 
AGAGTTCTCT ATGAGAAGTT CTTAGAATTC AACCCAGCAA ACTGCACAAC ATGGATGAAG 
TATGCAGAGC TTGAGACTAT TCTTGGAGAT ATCGACAGAT CAAGAGCTGT CTATGAACTT 
GCCATCAGTC AGCCTAGACT TGACATGCCA GAGGTTTTGT GGAAATCTTT CATTGACTTT 
GAGGTGGAGC AAGAAGAATG GGATAACTCG AGAGCTCTGT ACAGGAGACT ACTGGAGAGG 
ACACAACATG TTAAGGTCTG GATAAGTTTT GCTAAATGCG AGCTGTCGGT TGGATCAGAT 
GACTGTGTCT TAAGATCAAG ACAGGTCTAT GATGAAGCCA ACAAGGCGTT GAAGCATGTG 
GAGGAGAAAG AAGAGAGACT GATGCTACTA GAGGCATGGC AGGAGTTTGA GAATGAATTT 
GGAGACGATG AGAGTGTAGA ACAGGTTCAA GAACAGATGC CTAACAAGGT CAAGAAGAGG 
AGAAAGATTC AGACAGAAGA TGGGTCTGAT GCAGGATGGG AAGAGTACTA CGACTACATC 
TTTCCTTCAG ATGAGACCAA CCAATCCAAT CTTAAGCTGC TTGCTATGGC TAAGATGTGG 
AAACAGAAGA TGGCTGAAAT CAATGACACC TCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC100892953 cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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