EMILIN2 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene
Clones
gRNAs

Summary

Gene Symbol	EMILIN2
Entrez Gene ID	100922183
Full Name	elastin microfibril interfacer 2
Gene Type	protein-coding
Organism	Sarcophilus harrisii(Tasmanian devil)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003760037.2	XP_003760085.1	EMILIN-2
XM_023496221.1	XP_023351989.1	EMILIN-2
XM_031946586.1	XP_031802446.1	EMILIN-2

Pathways from BioSystems
Gene Ontology

The following EMILIN2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EMILIN2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa121685	XM_003760037.2 Latest version!	Sarcophilus harrisii elastin microfibril interfacer 2 (EMILIN2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OSa158633	XM_023496221.1 Latest version!	Sarcophilus harrisii elastin microfibril interfacer 2 (EMILIN2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OSa221392	XM_031946586.1 Latest version!	Sarcophilus harrisii elastin microfibril interfacer 2 (EMILIN2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EMILIN2 ( XM_003760037.2 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_003760085.1 Sarcophilus harrisii elastin microfibril interfacer 2 (EMILIN2), mRNA.

Price & Availability↑

CloneID	OSa121685
Clone ID Related Accession (Same CDS sequence)	XM_003760037.2
Accession Version	XM_003760037.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1431532800000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	EMILIN-2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003823318.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 15, 2015 this sequence version replaced XM_003760037.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sarcophilus harrisii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 8% of CDS bases ##RefSeq-Attributes-END##

ATGGCATTTT CCTGTGGGTG GCAATCACAT TATTTTGATT GCAAAACAGA CACCAGCTTC 
ATGGTGCTGC CCTTCACTGC AGCCACTCCA GAAAAGTGTA TCCAGCTCCG ACTTCATACG 
AATCCCCTTG GAGGCGTGGA CTCCCTCCCT CCCCATCCCC GAATGCACTG GGAGCAGCAA 
AAGTCCCCGG GGTGCTGCGG GGTGACACGT AGACTCCCCG CGCCTAACCT AGCCAGCATC 
TCCTGGATTG GGGGCTGGTT TGGGGAGCCT ATCCGGAGAG CATTAATCCT TTTCTCTCCT 
CCCCGCCCCA GGAACTGGTG CGCCTACATA GTGAACAAGA ATGTAAGCTG CTCTGTGCTG 
GAGGGAACTG AGAGTTTTAT TCAAGCTCAG TACAAATGTG CCTGGAACCA GATTCCCTGT 
CCGTCCACGC CGGTGTATCG AGTTAATTTC AGACCCAGAT ATACTATTAA GTTCAAGACA 
GTCACACAAC TGGAATGGAG ATGCTGCCCT GGGTTTAAAG GGGGTGACTG CCAAGAAGGG 
CCTAAAGAAC CAGCAAAATC TCCTCTGCCC ACACCTGCCC GGCCCCGGAA TACTATGAAA 
AAAGCCACGG ATACTGAACC AAAGCAAGTC ACAGAACCAA AGAAAACAGC AGAACCAAGA 
CACACAACAG AACCAAGACA GACAGCAAGC CCCAAGCCAA GCCTGCAGGA GCAGCAGGAC 
AAGAAAATAC AAGTGCTTGA GGACAAAGTT TCCCGACTTA CAAGGACAGT CCTTGACCTT 
CAGACCTCCA TTGCAGGAGT AAATGAAAAT TTAAAACATG CTGTGCAGGA TGATGCCAGT 
AAAATGTTGG CATCCTGGCT CAACAGCCTT CACAACCCAC CAGGGCCAGA CAGTGTCGTG 
GGTGGCGAAA CAGAAACAAT TCAGCTTCCC GGGATCCTGG GTGGCAAGGA GTCGGGAATG 
AAGGACATTA AGTCTGAGTT GGCTGAGGTT AAGGATGTAT TGAAGACAAA GAGTGACAAG 
CTGGAGGAGC TGGATGGGAA AGTAAAGGGT TGTGAAGGAC AACTCAAGCA GCTTCAGGAA 
GCTGCCCAGG GGCCCACAGT GACCATCCCG ACCAATGAGT TCTACCAGGC TTATGTAGAT 
AGCAAATTTG AGGCTCTGAG AGAGGAGCTG ATGGAAGGAA TAGACAGGAA AATGGCAGAT 
CTGAAAAACT CCTGTGAGTA TAAACTGACT GGCATCCAGC AACAGTGTGA TGATTATGGA 
AGTAGCTACT TGGGCATGAT AGAACTTATT GGTGAAAAGG AAACGAACCT AAGAAAGGAA 
ATAAGTGACC TTCGAGCTCA GATACAGGTA GCACCATCAA AACAACCAAA TTGTTGTAAT 
CTGGATAAAA GTGATGACTT TGGACGAAAG ATTGAAGTTT TAGACGAGAA AATTGAGAGG 
GTTGCAGAAG CCACCAGAAT GCTCAATGGG AGGCTAGACA ATGAATTTGA CCATCTCACC 
ATACCAGAAC CAGATGAGAG TTTTGATGAA AGATGGAATG AGTTGGATGC AAGGATCAAT 
GTGACGGAGA AGAATGCAGA AGAACATTGT TTTTACATTG AGGAAACTCT GCGTGGTACT 
ATTAATGGTG AAGTGGATGA TCTGAAGCAA CTTTTGGATC AGAAAATTCA GTCTCTGGAG 
GATCGTCTGG GTAGCAGTCT CCTAGACATT GCTAATGGCA CTGACGTAAA ATTCATCAAT 
CTAGAGTCAG TGTTGCCAGG ATCTGGCAAT GGCCAATTAA CTACTGAACT TAATCATCTG 
AAGAATAAAA TCCAAGATGT GGAGAATCTT TGTCTGCAGA ATCTCCAGTT AGGGTCTCAT 
GAAAAAGAAA GTACTCCTCC CAGTAAGTAT GATGATCAGA GGATGGATAC CTCTGCACTT 
CTAAAGGCTC TGAATGATAC AATGCATAGG AAGTTCAAAG AAACTGAACT TAATATTCAG 
AAAGTACAAC AAGATTTGAG TTTGCTCTTT TCTCATCTAA ATGACACTGA CAAAGATGTG 
AAAAATCTTC AGGGTGGGTT GAGCAACTGC CAAGAGCAAC TTGCGGATGC CATTTCTACA 
TGCAGGCAGG CAGAAGATAG TGTTTCCAGG AAGCTGGATG AGCTAGATAG GACCCCTGAA 
GAGCCCACTT CTTCCCAGGC CCCAGCGGGT AATTGCTGCA GTCATCTGGA GGTGAGGCTA 
CAGGGGCTAC AAAAGCAAAT CCTTTCCGAC CTGGACATCT GTAAGGAAAG CACTCAGGGA 
GTCCAGAGGG AAGTCATGGT TGTTGGAGGA AGAGTGTCTA ATGTTGAAAA AGCATGTGCC 
AAACTGGATT CCATCTCTGG GAGTCTACAC AGGATCAAGG AAGGATTGAA CAAGCATGTC 
AACAACCTGT GGAACTGTGT CAGTCAGATG AATGGCACGA TTGTAACCCA TTCCAAAGAC 
ATTTCTGGCC TCAAAAATTC TGTCCAGCAG ATCTATGGTC GTGTCTTCCA AATTACCACA 
GACCTTCAGG ATCTGGTGAA AGGTCAACCA GCAGGATCGG AAGCCCCATC AGAAATACCA 
CAGTTAATTC CACAACAACC AGAAAGCACC CCTGAACCAT CACAAATGGA AGTTCCTGGA 
GAATCATCAC ATCCAAAAAC ACCCCAGCAG CCTCCAGAAT CAAAAGGCCC CCTCCCTCCC 
AAGACTCCTG GGAAACCCTT CCAGCCAGGA ATACCTCAGC AGCCTTCACA ATCAAAGACT 
CCTCCACAGC CATCACCTCC CAAGCACATG CCATCCCATT CAGAGTTACC TGGGTGGACA 
GGTTTGCCTT TCCTACCAGG ATCTACAGGG GTCATCATGG AGACTGGGGA AGCTGGACCT 
CCTGGGAAAA TGGTGATGTC AGGGAGAGGC TTGCCCAATG GTGTTGATGG CCAGGACAGT 
CGGTGGCCCA TCCCAAGCTC TGAAGGATAT GCTGGAGCCC CAGGATACCC TAAGCGGCCA 
TCTCCTGTGG ATTCACAAGG GATTCCTACC GCCTCCCTGG TGTCCTTTTC TGCCGGTCTG 
ACTCAGAAAC CTTTTCCGAG TGAGGCGGGT GTGGTTCTCT TCAACAAAGT ACTTGTGAAT 
GATGGAGAAT TTTACGACCC TAACACTGGG ATCTTTACTG CCCCATATGA TGGTCGATAT 
CTGATCACTG CCATTCTCAC TCCTGAGAGA GATGAATATG TTGAAGCTGT CTTGTCGGTC 
TCTAATGCCA GCGTGGCCCA GCTGCACACA GCCGGATACA GAAGAGAACT CTTGGAATAT 
CACAGACCCC GAGGGGGGTT ACATACCTGT GGTGGAACAG GGACTTTCCA TCTCATCGTC 
CACTTGAAAG CAGGAGATGA GGTGAACATT GTGGTGACTG GGGGCAGGCT GGCCCACACA 
GACTTTGATG AAATGTACTC CACATTTAGT GGGGTCTTCC TCTATCCTTT CCTTTCCCAC 
ATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003760085.1
CDS	1..3486
Translation	MAFSCGWQSHYFDCKTDTSFMVLPFTAATPEKCIQLRLHTNPLGGVDSLPPHPRMHWEQQKSPGCCGVTRRLPAPNLASISWIGGWFGEPIRRALILFSPPRPRNWCAYIVNKNVSCSVLEGTESFIQAQYKCAWNQIPCPSTPVYRVNFRPRYTIKFKTVTQLEWRCCPGFKGGDCQEGPKEPAKSPLPTPARPRNTMKKATDTEPKQVTEPKKTAEPRHTTEPRQTASPKPSLQEQQDKKIQVLEDKVSRLTRTVLDLQTSIAGVNENLKHAVQDDASKMLASWLNSLHNPPGPDSVVGGETETIQLPGILGGKESGMKDIKSELAEVKDVLKTKSDKLEELDGKVKGCEGQLKQLQEAAQGPTVTIPTNEFYQAYVDSKFEALREELMEGIDRKMADLKNSCEYKLTGIQQQCDDYGSSYLGMIELIGEKETNLRKEISDLRAQIQVAPSKQPNCCNLDKSDDFGRKIEVLDEKIERVAEATRMLNGRLDNEFDHLTIPEPDESFDERWNELDARINVTEKNAEEHCFYIEETLRGTINGEVDDLKQLLDQKIQSLEDRLGSSLLDIANGTDVKFINLESVLPGSGNGQLTTELNHLKNKIQDVENLCLQNLQLGSHEKESTPPSKYDDQRMDTSALLKALNDTMHRKFKETELNIQKVQQDLSLLFSHLNDTDKDVKNLQGGLSNCQEQLADAISTCRQAEDSVSRKLDELDRTPEEPTSSQAPAGNCCSHLEVRLQGLQKQILSDLDICKESTQGVQREVMVVGGRVSNVEKACAKLDSISGSLHRIKEGLNKHVNNLWNCVSQMNGTIVTHSKDISGLKNSVQQIYGRVFQITTDLQDLVKGQPAGSEAPSEIPQLIPQQPESTPEPSQMEVPGESSHPKTPQQPPESKGPLPPKTPGKPFQPGIPQQPSQSKTPPQPSPPKHMPSHSELPGWTGLPFLPGSTGVIMETGEAGPPGKMVMSGRGLPNGVDGQDSRWPIPSSEGYAGAPGYPKRPSPVDSQGIPTASLVSFSAGLTQKPFPSEAGVVLFNKVLVNDGEFYDPNTGIFTAPYDGRYLITAILTPERDEYVEAVLSVSNASVAQLHTAGYRRELLEYHRPRGGLHTCGGTGTFHLIVHLKAGDEVNIVVTGGRLAHTDFDEMYSTFSGVFLYPFLSHI

Target ORF information:

RefSeq Version	XM_003760037.2
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii elastin microfibril interfacer 2 (EMILIN2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003760037.2

ORF Insert Sequence:

ATGGCATTTT CCTGTGGGTG GCAATCACAT TATTTTGATT GCAAAACAGA CACCAGCTTC 
ATGGTGCTGC CCTTCACTGC AGCCACTCCA GAAAAGTGTA TCCAGCTCCG ACTTCATACG 
AATCCCCTTG GAGGCGTGGA CTCCCTCCCT CCCCATCCCC GAATGCACTG GGAGCAGCAA 
AAGTCCCCGG GGTGCTGCGG GGTGACACGT AGACTCCCCG CGCCTAACCT AGCCAGCATC 
TCCTGGATTG GGGGCTGGTT TGGGGAGCCT ATCCGGAGAG CATTAATCCT TTTCTCTCCT 
CCCCGCCCCA GGAACTGGTG CGCCTACATA GTGAACAAGA ATGTAAGCTG CTCTGTGCTG 
GAGGGAACTG AGAGTTTTAT TCAAGCTCAG TACAAATGTG CCTGGAACCA GATTCCCTGT 
CCGTCCACGC CGGTGTATCG AGTTAATTTC AGACCCAGAT ATACTATTAA GTTCAAGACA 
GTCACACAAC TGGAATGGAG ATGCTGCCCT GGGTTTAAAG GGGGTGACTG CCAAGAAGGG 
CCTAAAGAAC CAGCAAAATC TCCTCTGCCC ACACCTGCCC GGCCCCGGAA TACTATGAAA 
AAAGCCACGG ATACTGAACC AAAGCAAGTC ACAGAACCAA AGAAAACAGC AGAACCAAGA 
CACACAACAG AACCAAGACA GACAGCAAGC CCCAAGCCAA GCCTGCAGGA GCAGCAGGAC 
AAGAAAATAC AAGTGCTTGA GGACAAAGTT TCCCGACTTA CAAGGACAGT CCTTGACCTT 
CAGACCTCCA TTGCAGGAGT AAATGAAAAT TTAAAACATG CTGTGCAGGA TGATGCCAGT 
AAAATGTTGG CATCCTGGCT CAACAGCCTT CACAACCCAC CAGGGCCAGA CAGTGTCGTG 
GGTGGCGAAA CAGAAACAAT TCAGCTTCCC GGGATCCTGG GTGGCAAGGA GTCGGGAATG 
AAGGACATTA AGTCTGAGTT GGCTGAGGTT AAGGATGTAT TGAAGACAAA GAGTGACAAG 
CTGGAGGAGC TGGATGGGAA AGTAAAGGGT TGTGAAGGAC AACTCAAGCA GCTTCAGGAA 
GCTGCCCAGG GGCCCACAGT GACCATCCCG ACCAATGAGT TCTACCAGGC TTATGTAGAT 
AGCAAATTTG AGGCTCTGAG AGAGGAGCTG ATGGAAGGAA TAGACAGGAA AATGGCAGAT 
CTGAAAAACT CCTGTGAGTA TAAACTGACT GGCATCCAGC AACAGTGTGA TGATTATGGA 
AGTAGCTACT TGGGCATGAT AGAACTTATT GGTGAAAAGG AAACGAACCT AAGAAAGGAA 
ATAAGTGACC TTCGAGCTCA GATACAGGTA GCACCATCAA AACAACCAAA TTGTTGTAAT 
CTGGATAAAA GTGATGACTT TGGACGAAAG ATTGAAGTTT TAGACGAGAA AATTGAGAGG 
GTTGCAGAAG CCACCAGAAT GCTCAATGGG AGGCTAGACA ATGAATTTGA CCATCTCACC 
ATACCAGAAC CAGATGAGAG TTTTGATGAA AGATGGAATG AGTTGGATGC AAGGATCAAT 
GTGACGGAGA AGAATGCAGA AGAACATTGT TTTTACATTG AGGAAACTCT GCGTGGTACT 
ATTAATGGTG AAGTGGATGA TCTGAAGCAA CTTTTGGATC AGAAAATTCA GTCTCTGGAG 
GATCGTCTGG GTAGCAGTCT CCTAGACATT GCTAATGGCA CTGACGTAAA ATTCATCAAT 
CTAGAGTCAG TGTTGCCAGG ATCTGGCAAT GGCCAATTAA CTACTGAACT TAATCATCTG 
AAGAATAAAA TCCAAGATGT GGAGAATCTT TGTCTGCAGA ATCTCCAGTT AGGGTCTCAT 
GAAAAAGAAA GTACTCCTCC CAGTAAGTAT GATGATCAGA GGATGGATAC CTCTGCACTT 
CTAAAGGCTC TGAATGATAC AATGCATAGG AAGTTCAAAG AAACTGAACT TAATATTCAG 
AAAGTACAAC AAGATTTGAG TTTGCTCTTT TCTCATCTAA ATGACACTGA CAAAGATGTG 
AAAAATCTTC AGGGTGGGTT GAGCAACTGC CAAGAGCAAC TTGCGGATGC CATTTCTACA 
TGCAGGCAGG CAGAAGATAG TGTTTCCAGG AAGCTGGATG AGCTAGATAG GACCCCTGAA 
GAGCCCACTT CTTCCCAGGC CCCAGCGGGT AATTGCTGCA GTCATCTGGA GGTGAGGCTA 
CAGGGGCTAC AAAAGCAAAT CCTTTCCGAC CTGGACATCT GTAAGGAAAG CACTCAGGGA 
GTCCAGAGGG AAGTCATGGT TGTTGGAGGA AGAGTGTCTA ATGTTGAAAA AGCATGTGCC 
AAACTGGATT CCATCTCTGG GAGTCTACAC AGGATCAAGG AAGGATTGAA CAAGCATGTC 
AACAACCTGT GGAACTGTGT CAGTCAGATG AATGGCACGA TTGTAACCCA TTCCAAAGAC 
ATTTCTGGCC TCAAAAATTC TGTCCAGCAG ATCTATGGTC GTGTCTTCCA AATTACCACA 
GACCTTCAGG ATCTGGTGAA AGGTCAACCA GCAGGATCGG AAGCCCCATC AGAAATACCA 
CAGTTAATTC CACAACAACC AGAAAGCACC CCTGAACCAT CACAAATGGA AGTTCCTGGA 
GAATCATCAC ATCCAAAAAC ACCCCAGCAG CCTCCAGAAT CAAAAGGCCC CCTCCCTCCC 
AAGACTCCTG GGAAACCCTT CCAGCCAGGA ATACCTCAGC AGCCTTCACA ATCAAAGACT 
CCTCCACAGC CATCACCTCC CAAGCACATG CCATCCCATT CAGAGTTACC TGGGTGGACA 
GGTTTGCCTT TCCTACCAGG ATCTACAGGG GTCATCATGG AGACTGGGGA AGCTGGACCT 
CCTGGGAAAA TGGTGATGTC AGGGAGAGGC TTGCCCAATG GTGTTGATGG CCAGGACAGT 
CGGTGGCCCA TCCCAAGCTC TGAAGGATAT GCTGGAGCCC CAGGATACCC TAAGCGGCCA 
TCTCCTGTGG ATTCACAAGG GATTCCTACC GCCTCCCTGG TGTCCTTTTC TGCCGGTCTG 
ACTCAGAAAC CTTTTCCGAG TGAGGCGGGT GTGGTTCTCT TCAACAAAGT ACTTGTGAAT 
GATGGAGAAT TTTACGACCC TAACACTGGG ATCTTTACTG CCCCATATGA TGGTCGATAT 
CTGATCACTG CCATTCTCAC TCCTGAGAGA GATGAATATG TTGAAGCTGT CTTGTCGGTC 
TCTAATGCCA GCGTGGCCCA GCTGCACACA GCCGGATACA GAAGAGAACT CTTGGAATAT 
CACAGACCCC GAGGGGGGTT ACATACCTGT GGTGGAACAG GGACTTTCCA TCTCATCGTC 
CACTTGAAAG CAGGAGATGA GGTGAACATT GTGGTGACTG GGGGCAGGCT GGCCCACACA 
GACTTTGATG AAATGTACTC CACATTTAGT GGGGTCTTCC TCTATCCTTT CCTTTCCCAC 
ATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EMILIN2 ( XM_023496221.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_023351989.1 Sarcophilus harrisii elastin microfibril interfacer 2 (EMILIN2), mRNA.

Price & Availability↑

CloneID	OSa158633
Clone ID Related Accession (Same CDS sequence)	XM_023496221.1
Accession Version	XM_023496221.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3312bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515513600000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	EMILIN-2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003823318.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 3% of CDS bases ##RefSeq-Attributes-END##

ATGGCATTTT CCTGTGGGTG GCAATCACAT TATTTTGATT GCAAAACAGA CACCAGCTTC 
ATGGTGCTGC CCTTCACTGC AGCCACTCCA GAAAAGTGTA TCCAGCTCCG ACTTCACCGG 
CCCGGCGTTC GGAACAAGAA CTGGTGCGCC TACATAGTGA ACAAGAATGT AAGCTGCTCT 
GTGCTGGAGG GAACTGAGAG TTTTATTCAA GCTCAGTACA AATGTGCCTG GAACCAGATT 
CCCTGTCCGT CCACGCCGGT GTATCGAGTT AATTTCAGAC CCAGATATAC TATTAAGTTC 
AAGACAGTCA CACAACTGGA ATGGAGATGC TGCCCTGGGT TTAAAGGGGG TGACTGCCAA 
GAAGGGCCTA AAGAACCAGC AAAATCTCCT CTGCCCACAC CTGCCCGGCC CCGGAATACT 
ATGAAAAAAG CCACGGATAC TGAACCAAAG CAAGTCACAG AACCAAAGAA AACAGCAGAA 
CCAAGACACA CAACAGAACC AAGACAGACA GCAAGCCCCA AGCCAAGCCT GCAGGAGCAG 
CAGGACAAGA AAATACAAGT GCTTGAGGAC AAAGTTTCCC GACTTACAAG GACAGTCCTT 
GACCTTCAGA CCTCCATTGC AGGAGTAAAT GAAAATTTAA AACATGCTGT GCAGGATGAT 
GCCAGTAAAA TGTTGGCATC CTGGCTCAAC AGCCTTCACA ACCCACCAGG GCCAGACAGT 
GTCGTGGGTG GCGAAACAGA AACAATTCAG CTTCCCGGGA TCCTGGGTGG CAAGGAGTCG 
GGAATGAAGG ACATTAAGTC TGAGTTGGCT GAGGTTAAGG ATGTATTGAA GACAAAGAGT 
GACAAGCTGG AGGAGCTGGA TGGGAAAGTA AAGGGTTGTG AAGGACAACT CAAGCAGCTT 
CAGGAAGCTG CCCAGGGGCC CACAGTGACC ATCCCGACCA ATGAGTTCTA CCAGGCTTAT 
GTAGATAGCA AATTTGAGGC TCTGAGAGAG GAGCTGATGG AAGGAATAGA CAGGAAAATG 
GCAGATCTGA AAAACTCCTG TGAGTATAAA CTGACTGGCA TCCAGCAACA GTGTGATGAT 
TATGGAAGTA GCTACTTGGG CATGATAGAA CTTATTGGTG AAAAGGAAAC GAACCTAAGA 
AAGGAAATAA GTGACCTTCG AGCTCAGATA CAGGTAGCAC CATCAAAACA ACCAAATTGT 
TGTAATCTGG ATAAAAGTGA TGACTTTGGA CGAAAGATTG AAGTTTTAGA CGAGAAAATT 
GAGAGGGTTG CAGAAGCCAC CAGAATGCTC AATGGGAGGC TAGACAATGA ATTTGACCAT 
CTCACCATAC CAGAACCAGA TGAGAGTTTT GATGAAAGAT GGAATGAGTT GGATGCAAGG 
ATCAATGTGA CGGAGAAGAA TGCAGAAGAA CATTGTTTTT ACATTGAGGA AACTCTGCGT 
GGTACTATTA ATGGTGAAGT GGATGATCTG AAGCAACTTT TGGATCAGAA AATTCAGTCT 
CTGGAGGATC GTCTGGGTAG CAGTCTCCTA GACATTGCTA ATGGCACTGA CGTAAAATTC 
ATCAATCTAG AGTCAGTGTT GCCAGGATCT GGCAATGGCC AATTAACTAC TGAACTTAAT 
CATCTGAAGA ATAAAATCCA AGATGTGGAG AATCTTTGTC TGCAGAATCT CCAGTTAGGG 
TCTCATGAAA AAGAAAGTAC TCCTCCCAGT AAGTATGATG ATCAGAGGAT GGATACCTCT 
GCACTTCTAA AGGCTCTGAA TGATACAATG CATAGGAAGT TCAAAGAAAC TGAACTTAAT 
ATTCAGAAAG TACAACAAGA TTTGAGTTTG CTCTTTTCTC ATCTAAATGA CACTGACAAA 
GATGTGAAAA ATCTTCAGGG TGGGTTGAGC AACTGCCAAG AGCAACTTGC GGATGCCATT 
TCTACATGCA GGCAGGCAGA AGATAGTGTT TCCAGGAAGC TGGATGAGCT AGATAGGACC 
CCTGAAGAGC CCACTTCTTC CCAGGCCCCA GCGGGTAATT GCTGCAGTCA TCTGGAGGTG 
AGGCTACAGG GGCTACAAAA GCAAATCCTT TCCGACCTGG ACATCTGTAA GGAAAGCACT 
CAGGGAGTCC AGAGGGAAGT CATGGTTGTT GGAGGAAGAG TGTCTAATGT TGAAAAAGCA 
TGTGCCAAAC TGGATTCCAT CTCTGGGAGT CTACACAGGA TCAAGGAAGG ATTGAACAAG 
CATGTCAACA ACCTGTGGAA CTGTGTCAGT CAGATGAATG GCACGATTGT AACCCATTCC 
AAAGACATTT CTGGCCTCAA AAATTCTGTC CAGCAGATCT ATGGTCGTGT CTTCCAAATT 
ACCACAGACC TTCAGGATCT GGTGAAAGGT CAACCAGCAG GATCGGAAGC CCCATCAGAA 
ATACCACAGT TAATTCCACA ACAACCAGAA AGCACCCCTG AACCATCACA AATGGAAGTT 
CCTGGAGAAT CATCACATCC AAAAACACCC CAGCAGCCTC CAGAATCAAA AGGCCCCCTC 
CCTCCCAAGA CTCCTGGGAA ACCCTTCCAG CCAGGAATAC CTCAGCAGCC TTCACAATCA 
AAGACTCCTC CACAGCCATC ACCTCCCAAG CACATGCCAT CCCATTCAGA GTTACCTGGG 
TGGACAGGTT TGCCTTTCCT ACCAGGATCT ACAGGGGTCA TCATGGAGAC TGGGGAAGCT 
GGACCTCCTG GGAAAATGGT GATGTCAGGG AGAGGCTTGC CCAATGGTGT TGATGGCCAG 
GACAGTCGGT GGCCCATCCC AAGCTCTGAA GGATATGCTG GAGCCCCAGG ATACCCTAAG 
CGGCCATCTC CTGTGGATTC ACAAGGGATT CCTACCGCCT CCCTGGTGTC CTTTTCTGCC 
GGTCTGACTC AGAAACCTTT TCCGAGTGAG GCGGGTGTGG TTCTCTTCAA CAAAGTACTT 
GTGAATGATG GAGAATTTTA CGACCCTAAC ACTGGGATCT TTACTGCCCC ATATGATGGT 
CGATATCTGA TCACTGCCAT TCTCACTCCT GAGAGAGATG AATATGTTGA AGCTGTCTTG 
TCGGTCTCTA ATGCCAGCGT GGCCCAGCTG CACACAGCCG GATACAGAAG AGAACTCTTG 
GAATATCACA GACCCCGAGG GGGGTTACAT ACCTGTGGTG GAACAGGGAC TTTCCATCTC 
ATCGTCCACT TGAAAGCAGG AGATGAGGTG AACATTGTGG TGACTGGGGG CAGGCTGGCC 
CACACAGACT TTGATGAAAT GTACTCCACA TTTAGTGGGG TCTTCCTCTA TCCTTTCCTT 
TCCCACATCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023351989.1
CDS	1..3312
Translation	MAFSCGWQSHYFDCKTDTSFMVLPFTAATPEKCIQLRLHRPGVRNKNWCAYIVNKNVSCSVLEGTESFIQAQYKCAWNQIPCPSTPVYRVNFRPRYTIKFKTVTQLEWRCCPGFKGGDCQEGPKEPAKSPLPTPARPRNTMKKATDTEPKQVTEPKKTAEPRHTTEPRQTASPKPSLQEQQDKKIQVLEDKVSRLTRTVLDLQTSIAGVNENLKHAVQDDASKMLASWLNSLHNPPGPDSVVGGETETIQLPGILGGKESGMKDIKSELAEVKDVLKTKSDKLEELDGKVKGCEGQLKQLQEAAQGPTVTIPTNEFYQAYVDSKFEALREELMEGIDRKMADLKNSCEYKLTGIQQQCDDYGSSYLGMIELIGEKETNLRKEISDLRAQIQVAPSKQPNCCNLDKSDDFGRKIEVLDEKIERVAEATRMLNGRLDNEFDHLTIPEPDESFDERWNELDARINVTEKNAEEHCFYIEETLRGTINGEVDDLKQLLDQKIQSLEDRLGSSLLDIANGTDVKFINLESVLPGSGNGQLTTELNHLKNKIQDVENLCLQNLQLGSHEKESTPPSKYDDQRMDTSALLKALNDTMHRKFKETELNIQKVQQDLSLLFSHLNDTDKDVKNLQGGLSNCQEQLADAISTCRQAEDSVSRKLDELDRTPEEPTSSQAPAGNCCSHLEVRLQGLQKQILSDLDICKESTQGVQREVMVVGGRVSNVEKACAKLDSISGSLHRIKEGLNKHVNNLWNCVSQMNGTIVTHSKDISGLKNSVQQIYGRVFQITTDLQDLVKGQPAGSEAPSEIPQLIPQQPESTPEPSQMEVPGESSHPKTPQQPPESKGPLPPKTPGKPFQPGIPQQPSQSKTPPQPSPPKHMPSHSELPGWTGLPFLPGSTGVIMETGEAGPPGKMVMSGRGLPNGVDGQDSRWPIPSSEGYAGAPGYPKRPSPVDSQGIPTASLVSFSAGLTQKPFPSEAGVVLFNKVLVNDGEFYDPNTGIFTAPYDGRYLITAILTPERDEYVEAVLSVSNASVAQLHTAGYRRELLEYHRPRGGLHTCGGTGTFHLIVHLKAGDEVNIVVTGGRLAHTDFDEMYSTFSGVFLYPFLSHI

Target ORF information:

RefSeq Version	XM_023496221.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii elastin microfibril interfacer 2 (EMILIN2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023496221.1

ORF Insert Sequence:

ATGGCATTTT CCTGTGGGTG GCAATCACAT TATTTTGATT GCAAAACAGA CACCAGCTTC 
ATGGTGCTGC CCTTCACTGC AGCCACTCCA GAAAAGTGTA TCCAGCTCCG ACTTCACCGG 
CCCGGCGTTC GGAACAAGAA CTGGTGCGCC TACATAGTGA ACAAGAATGT AAGCTGCTCT 
GTGCTGGAGG GAACTGAGAG TTTTATTCAA GCTCAGTACA AATGTGCCTG GAACCAGATT 
CCCTGTCCGT CCACGCCGGT GTATCGAGTT AATTTCAGAC CCAGATATAC TATTAAGTTC 
AAGACAGTCA CACAACTGGA ATGGAGATGC TGCCCTGGGT TTAAAGGGGG TGACTGCCAA 
GAAGGGCCTA AAGAACCAGC AAAATCTCCT CTGCCCACAC CTGCCCGGCC CCGGAATACT 
ATGAAAAAAG CCACGGATAC TGAACCAAAG CAAGTCACAG AACCAAAGAA AACAGCAGAA 
CCAAGACACA CAACAGAACC AAGACAGACA GCAAGCCCCA AGCCAAGCCT GCAGGAGCAG 
CAGGACAAGA AAATACAAGT GCTTGAGGAC AAAGTTTCCC GACTTACAAG GACAGTCCTT 
GACCTTCAGA CCTCCATTGC AGGAGTAAAT GAAAATTTAA AACATGCTGT GCAGGATGAT 
GCCAGTAAAA TGTTGGCATC CTGGCTCAAC AGCCTTCACA ACCCACCAGG GCCAGACAGT 
GTCGTGGGTG GCGAAACAGA AACAATTCAG CTTCCCGGGA TCCTGGGTGG CAAGGAGTCG 
GGAATGAAGG ACATTAAGTC TGAGTTGGCT GAGGTTAAGG ATGTATTGAA GACAAAGAGT 
GACAAGCTGG AGGAGCTGGA TGGGAAAGTA AAGGGTTGTG AAGGACAACT CAAGCAGCTT 
CAGGAAGCTG CCCAGGGGCC CACAGTGACC ATCCCGACCA ATGAGTTCTA CCAGGCTTAT 
GTAGATAGCA AATTTGAGGC TCTGAGAGAG GAGCTGATGG AAGGAATAGA CAGGAAAATG 
GCAGATCTGA AAAACTCCTG TGAGTATAAA CTGACTGGCA TCCAGCAACA GTGTGATGAT 
TATGGAAGTA GCTACTTGGG CATGATAGAA CTTATTGGTG AAAAGGAAAC GAACCTAAGA 
AAGGAAATAA GTGACCTTCG AGCTCAGATA CAGGTAGCAC CATCAAAACA ACCAAATTGT 
TGTAATCTGG ATAAAAGTGA TGACTTTGGA CGAAAGATTG AAGTTTTAGA CGAGAAAATT 
GAGAGGGTTG CAGAAGCCAC CAGAATGCTC AATGGGAGGC TAGACAATGA ATTTGACCAT 
CTCACCATAC CAGAACCAGA TGAGAGTTTT GATGAAAGAT GGAATGAGTT GGATGCAAGG 
ATCAATGTGA CGGAGAAGAA TGCAGAAGAA CATTGTTTTT ACATTGAGGA AACTCTGCGT 
GGTACTATTA ATGGTGAAGT GGATGATCTG AAGCAACTTT TGGATCAGAA AATTCAGTCT 
CTGGAGGATC GTCTGGGTAG CAGTCTCCTA GACATTGCTA ATGGCACTGA CGTAAAATTC 
ATCAATCTAG AGTCAGTGTT GCCAGGATCT GGCAATGGCC AATTAACTAC TGAACTTAAT 
CATCTGAAGA ATAAAATCCA AGATGTGGAG AATCTTTGTC TGCAGAATCT CCAGTTAGGG 
TCTCATGAAA AAGAAAGTAC TCCTCCCAGT AAGTATGATG ATCAGAGGAT GGATACCTCT 
GCACTTCTAA AGGCTCTGAA TGATACAATG CATAGGAAGT TCAAAGAAAC TGAACTTAAT 
ATTCAGAAAG TACAACAAGA TTTGAGTTTG CTCTTTTCTC ATCTAAATGA CACTGACAAA 
GATGTGAAAA ATCTTCAGGG TGGGTTGAGC AACTGCCAAG AGCAACTTGC GGATGCCATT 
TCTACATGCA GGCAGGCAGA AGATAGTGTT TCCAGGAAGC TGGATGAGCT AGATAGGACC 
CCTGAAGAGC CCACTTCTTC CCAGGCCCCA GCGGGTAATT GCTGCAGTCA TCTGGAGGTG 
AGGCTACAGG GGCTACAAAA GCAAATCCTT TCCGACCTGG ACATCTGTAA GGAAAGCACT 
CAGGGAGTCC AGAGGGAAGT CATGGTTGTT GGAGGAAGAG TGTCTAATGT TGAAAAAGCA 
TGTGCCAAAC TGGATTCCAT CTCTGGGAGT CTACACAGGA TCAAGGAAGG ATTGAACAAG 
CATGTCAACA ACCTGTGGAA CTGTGTCAGT CAGATGAATG GCACGATTGT AACCCATTCC 
AAAGACATTT CTGGCCTCAA AAATTCTGTC CAGCAGATCT ATGGTCGTGT CTTCCAAATT 
ACCACAGACC TTCAGGATCT GGTGAAAGGT CAACCAGCAG GATCGGAAGC CCCATCAGAA 
ATACCACAGT TAATTCCACA ACAACCAGAA AGCACCCCTG AACCATCACA AATGGAAGTT 
CCTGGAGAAT CATCACATCC AAAAACACCC CAGCAGCCTC CAGAATCAAA AGGCCCCCTC 
CCTCCCAAGA CTCCTGGGAA ACCCTTCCAG CCAGGAATAC CTCAGCAGCC TTCACAATCA 
AAGACTCCTC CACAGCCATC ACCTCCCAAG CACATGCCAT CCCATTCAGA GTTACCTGGG 
TGGACAGGTT TGCCTTTCCT ACCAGGATCT ACAGGGGTCA TCATGGAGAC TGGGGAAGCT 
GGACCTCCTG GGAAAATGGT GATGTCAGGG AGAGGCTTGC CCAATGGTGT TGATGGCCAG 
GACAGTCGGT GGCCCATCCC AAGCTCTGAA GGATATGCTG GAGCCCCAGG ATACCCTAAG 
CGGCCATCTC CTGTGGATTC ACAAGGGATT CCTACCGCCT CCCTGGTGTC CTTTTCTGCC 
GGTCTGACTC AGAAACCTTT TCCGAGTGAG GCGGGTGTGG TTCTCTTCAA CAAAGTACTT 
GTGAATGATG GAGAATTTTA CGACCCTAAC ACTGGGATCT TTACTGCCCC ATATGATGGT 
CGATATCTGA TCACTGCCAT TCTCACTCCT GAGAGAGATG AATATGTTGA AGCTGTCTTG 
TCGGTCTCTA ATGCCAGCGT GGCCCAGCTG CACACAGCCG GATACAGAAG AGAACTCTTG 
GAATATCACA GACCCCGAGG GGGGTTACAT ACCTGTGGTG GAACAGGGAC TTTCCATCTC 
ATCGTCCACT TGAAAGCAGG AGATGAGGTG AACATTGTGG TGACTGGGGG CAGGCTGGCC 
CACACAGACT TTGATGAAAT GTACTCCACA TTTAGTGGGG TCTTCCTCTA TCCTTTCCTT 
TCCCACATCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EMILIN2 ( XM_031946586.1 ) cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil) -> XP_031802446.1 Sarcophilus harrisii elastin microfibril interfacer 2 (EMILIN2), mRNA.

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CloneID	OSa221392
Clone ID Related Accession (Same CDS sequence)	XM_031946586.1
Accession Version	XM_031946586.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Sarcophilus harrisii(Tasmanian devil)
Product	EMILIN-2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_045426.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Sarcophilus harrisii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGCCCT GCGGCTGGGC GTCGGTGCTG GTGCTCTTGG CCCTTCAAGG GGACTGGCTC 
ACCCAAGCGA CCCCCCATAT GCGGCAGACC AGCCGGCCCG GCGTTCGGAA CAAGAACTGG 
TGCGCCTACA TAGTGAACAA GAATGTAAGC TGCTCTGTGC TGGAGGGAAC TGAGAGTTTT 
ATTCAAGCTC AGTACAAATG TGCCTGGAAC CAGATTCCCT GTCCGTCCAC GCCGGTGTAT 
CGAGTTAATT TCAGACCCAG ATATACTATT AAGTTCAAGA CAGTCACACA ACTGGAATGG 
AGATGCTGCC CTGGGTTTAA AGGGGGTGAC TGCCAAGAAG GGCCTAAAGA ACCAGCAAAA 
TCTCCTCTGC CCACACCTGC CCGGCCCCGG AATACTATGA AAAAAGCCAC GGATACTGAA 
CCAAAGCAAG TCACAGAACC AAAGAAAACA GCAGAACCAA GACACACAAC AGAACCAAGA 
CAGACAGCAA GCCCCAAGCC AAGCCTGCAG GAGCAGCAGG ACAAGAAAAT ACAAGTGCTT 
GAGGACAAAG TTTCCCGACT TACAAGGACA GTCCTTGACC TTCAGACCTC CATTGCAGGA 
GTAAATGAAA ATTTAAAACA TGCTGTGCAG GATGATGCCA GTAAAATGTT GGCATCCTGG 
CTCAACAGCC TTCACAACCC ACCAGGGCCA GACAGTGTCG TGGGTGGCGA AACAGAAACA 
ATTCAGCTTC CCGGGATCCT GGGTGGCAAG GAGTCGGGAA TGAAGGACAT TAAGTCTGAG 
TTGGCTGAGG TTAAGGATGT ATTGAAGACA AAGAGTGACA AGCTGGAGGA GCTGGATGGG 
AAAGTAAAGG GTTGTGAAGG ACAACTCAAG CAGCTTCAGG AAGCTGCCCA GGGGCCCACA 
GTGACCATCC CGACCAATGA GTTCTACCAG GCTTATGTAG ATAGCAAATT TGAGGCTCTG 
AGAGAGGAGC TGATGGAAGG AATAGACAGG AAAATGGCAG ATCTGAAAAA CTCCTGTGAG 
TATAAACTGA CTGGCATCCA GCAACAGTGT GATGATTATG GAAGTAGCTA CTTGGGCATG 
ATAGAACTTA TTGGTGAAAA GGAAACGAAC CTAAGAAAGG AAATAAGTGA CCTTCGAGCT 
CAGATACAGG TAGCACCATC AAAACAACCA AATTGTTGTA ATCTGGATAA AAGTGATGAC 
TTTGGACGAA AGATTGAAGT TTTAGACGAG AAAATTGAGA GGGTTGCAGA AGCCACCAGA 
ATGCTCAATG GGAGGCTAGA CAATGAATTT GACCATCTCA CCATACCAGA ACCAGATGAG 
AGTTTTGATG AAAGATGGAA TGAGTTGGAT GCAAGGATCA ATGTGACGGA GAAGAATGCA 
GAAGAACATT GTTTTTACAT TGAGGAAACT CTGCGTGGTA CTATTAATGG TGAAGTGGAT 
GATCTGAAGC AACTTTTGGA TCAGAAAATT CAGTCTCTGG AGGATCGTCT GGGTAGCAGT 
CTCCTAGACA TTGCTAATGG CACTGACGTA AAATTCATCA ATCTAGAGTC AGTGTTGCCA 
GGATCTGGCA ATGGCCAATT AACTACTGAA CTTAATCATC TGAAGAATAA AATCCAAGAT 
GTGGAGAATC TTTGTCTGCA GAATCTCCAG TTAGGGTCTC ATGAAAAAGA AAGTACTCCT 
CCCAGTAAGT ATGATGATCA GAGGATGGAT ACCTCTGCAC TTCTAAAGGC TCTGAATGAT 
ACAATGCATA GGAAGTTCAA AGAAACTGAA CTTAATATTC AGAAAGTACA ACAAGATTTG 
AGTTTGCTCT TTTCTCATCT AAATGACACT GACAAAGATG TGAAAAATCT TCAGGGTGGG 
TTGAGCAACT GCCAAGAGCA ACTTGCGGAT GCCATTTCTA CATGCAGGCA GGCAGAAGAT 
AGTGTTTCCA GGAAGCTGGA TGAGCTAGAT AGGACCCCTG AAGAGCCCAC TTCTTCCCAG 
GCCCCAGCGG GTAATTGCTG CAGTCATCTG GAGGTGAGGC TACAGGGGCT ACAAAAGCAA 
ATCCTTTCCG ACCTGGACAT CTGTAAGGAA AGCACTCAGG GAGTCCAGAG GGAAGTCATG 
GTTGTTGGAG GAAGAGTGTC TAATGTTGAA AAAGCATGTG CCAAACTGGA TTCCATCTCT 
GGGAGTCTAC ACAGGATCAA GGAAGGATTG AACAAGCATG TCAACAACCT GTGGAACTGT 
GTCAGTCAGA TGAATGGCAC GATTGTAACC CATTCCAAAG ACATTTCTGG CCTCAAAAAT 
TCTGTCCAGC AGATCTATGG TCGTGTCTTC CAAATTACCA CAGACCTTCA GGATCTGGTG 
AAAGGTCAAC CAGCAGGATC GGAAGCCCCA TCAGAAATAC CACAGTTAAT TCCACAACAA 
CCAGAAAGCA CCCCTGAACC ATCACAAATG GAAGTTCCTG GAGAATCATC ACATCCAAAA 
ACACCCCAGC AGCCTCCAGA ATCAAAAGGC CCCCTCCCTC CCAAGACTCC TGGGAAACCC 
TTCCAGCCAG GAATACCTCA GCAGCCTTCA CAATCAAAGA CTCCTCCACA GCCATCACCT 
CCCAAGCACA TGCCATCCCA TTCAGAGTTA CCTGGGTGGA CAGGTTTGCC TTTCCTACCA 
GGATCTACAG GGGTCATCAT GGAGACTGGG GAAGCTGGAC CTCCTGGGAA AATGGTGATG 
TCAGGGAGAG GCTTGCCCAA TGGTGTTGAT GGCCAGGACA GTCGGTGGCC CATCCCAAGC 
TCTGAAGGAT ATGCTGGAGC CCCAGGATAC CCTAAGCGGC CATCTCCTGT GGATTCACAA 
GGGATTCCTA CCGCCTCCCT GGTGTCCTTT TCTGCCGGTC TGACTCAGAA ACCTTTTCCG 
AGTGAGGCGG GTGTGGTTCT CTTCAACAAA GTACTTGTGA ATGATGGAGA ATTTTACGAC 
CCTAACACTG GGATCTTTAC TGCCCCATAT GATGGTCGAT ATCTGATCAC TGCCATTCTC 
ACTCCTGAGA GAGATGAATA TGTTGAAGCT GTCTTGTCGG TCTCTAATGC CAGCGTGGCC 
CAGCTGCACA CAGCCGGATA CAGAAGAGAA CTCTTGGAAT ATCACAGACC CCGAGGGGGG 
TTACATACCT GTGGTGGAAC AGGGACTTTC CATCTCATCG TCCACTTGAA AGCAGGAGAT 
GAGGTGAACA TTGTGGTGAC TGGGGGCAGG CTGGCCCACA CAGACTTTGA TGAAATGTAC 
TCCACATTTA GTGGGGTCTT CCTCTATCCT TTCCTTTCCC ACATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031802446.1
CDS	465..3752
Translation	MWPCGWASVLVLLALQGDWLTQATPHMRQTSRPGVRNKNWCAYIVNKNVSCSVLEGTESFIQAQYKCAWNQIPCPSTPVYRVNFRPRYTIKFKTVTQLEWRCCPGFKGGDCQEGPKEPAKSPLPTPARPRNTMKKATDTEPKQVTEPKKTAEPRHTTEPRQTASPKPSLQEQQDKKIQVLEDKVSRLTRTVLDLQTSIAGVNENLKHAVQDDASKMLASWLNSLHNPPGPDSVVGGETETIQLPGILGGKESGMKDIKSELAEVKDVLKTKSDKLEELDGKVKGCEGQLKQLQEAAQGPTVTIPTNEFYQAYVDSKFEALREELMEGIDRKMADLKNSCEYKLTGIQQQCDDYGSSYLGMIELIGEKETNLRKEISDLRAQIQVAPSKQPNCCNLDKSDDFGRKIEVLDEKIERVAEATRMLNGRLDNEFDHLTIPEPDESFDERWNELDARINVTEKNAEEHCFYIEETLRGTINGEVDDLKQLLDQKIQSLEDRLGSSLLDIANGTDVKFINLESVLPGSGNGQLTTELNHLKNKIQDVENLCLQNLQLGSHEKESTPPSKYDDQRMDTSALLKALNDTMHRKFKETELNIQKVQQDLSLLFSHLNDTDKDVKNLQGGLSNCQEQLADAISTCRQAEDSVSRKLDELDRTPEEPTSSQAPAGNCCSHLEVRLQGLQKQILSDLDICKESTQGVQREVMVVGGRVSNVEKACAKLDSISGSLHRIKEGLNKHVNNLWNCVSQMNGTIVTHSKDISGLKNSVQQIYGRVFQITTDLQDLVKGQPAGSEAPSEIPQLIPQQPESTPEPSQMEVPGESSHPKTPQQPPESKGPLPPKTPGKPFQPGIPQQPSQSKTPPQPSPPKHMPSHSELPGWTGLPFLPGSTGVIMETGEAGPPGKMVMSGRGLPNGVDGQDSRWPIPSSEGYAGAPGYPKRPSPVDSQGIPTASLVSFSAGLTQKPFPSEAGVVLFNKVLVNDGEFYDPNTGIFTAPYDGRYLITAILTPERDEYVEAVLSVSNASVAQLHTAGYRRELLEYHRPRGGLHTCGGTGTFHLIVHLKAGDEVNIVVTGGRLAHTDFDEMYSTFSGVFLYPFLSHI

Target ORF information:

RefSeq Version	XM_031946586.1
Organism	Sarcophilus harrisii(Tasmanian devil)
Definition	Sarcophilus harrisii elastin microfibril interfacer 2 (EMILIN2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031946586.1

ORF Insert Sequence:

ATGTGGCCCT GCGGCTGGGC GTCGGTGCTG GTGCTCTTGG CCCTTCAAGG GGACTGGCTC 
ACCCAAGCGA CCCCCCATAT GCGGCAGACC AGCCGGCCCG GCGTTCGGAA CAAGAACTGG 
TGCGCCTACA TAGTGAACAA GAATGTAAGC TGCTCTGTGC TGGAGGGAAC TGAGAGTTTT 
ATTCAAGCTC AGTACAAATG TGCCTGGAAC CAGATTCCCT GTCCGTCCAC GCCGGTGTAT 
CGAGTTAATT TCAGACCCAG ATATACTATT AAGTTCAAGA CAGTCACACA ACTGGAATGG 
AGATGCTGCC CTGGGTTTAA AGGGGGTGAC TGCCAAGAAG GGCCTAAAGA ACCAGCAAAA 
TCTCCTCTGC CCACACCTGC CCGGCCCCGG AATACTATGA AAAAAGCCAC GGATACTGAA 
CCAAAGCAAG TCACAGAACC AAAGAAAACA GCAGAACCAA GACACACAAC AGAACCAAGA 
CAGACAGCAA GCCCCAAGCC AAGCCTGCAG GAGCAGCAGG ACAAGAAAAT ACAAGTGCTT 
GAGGACAAAG TTTCCCGACT TACAAGGACA GTCCTTGACC TTCAGACCTC CATTGCAGGA 
GTAAATGAAA ATTTAAAACA TGCTGTGCAG GATGATGCCA GTAAAATGTT GGCATCCTGG 
CTCAACAGCC TTCACAACCC ACCAGGGCCA GACAGTGTCG TGGGTGGCGA AACAGAAACA 
ATTCAGCTTC CCGGGATCCT GGGTGGCAAG GAGTCGGGAA TGAAGGACAT TAAGTCTGAG 
TTGGCTGAGG TTAAGGATGT ATTGAAGACA AAGAGTGACA AGCTGGAGGA GCTGGATGGG 
AAAGTAAAGG GTTGTGAAGG ACAACTCAAG CAGCTTCAGG AAGCTGCCCA GGGGCCCACA 
GTGACCATCC CGACCAATGA GTTCTACCAG GCTTATGTAG ATAGCAAATT TGAGGCTCTG 
AGAGAGGAGC TGATGGAAGG AATAGACAGG AAAATGGCAG ATCTGAAAAA CTCCTGTGAG 
TATAAACTGA CTGGCATCCA GCAACAGTGT GATGATTATG GAAGTAGCTA CTTGGGCATG 
ATAGAACTTA TTGGTGAAAA GGAAACGAAC CTAAGAAAGG AAATAAGTGA CCTTCGAGCT 
CAGATACAGG TAGCACCATC AAAACAACCA AATTGTTGTA ATCTGGATAA AAGTGATGAC 
TTTGGACGAA AGATTGAAGT TTTAGACGAG AAAATTGAGA GGGTTGCAGA AGCCACCAGA 
ATGCTCAATG GGAGGCTAGA CAATGAATTT GACCATCTCA CCATACCAGA ACCAGATGAG 
AGTTTTGATG AAAGATGGAA TGAGTTGGAT GCAAGGATCA ATGTGACGGA GAAGAATGCA 
GAAGAACATT GTTTTTACAT TGAGGAAACT CTGCGTGGTA CTATTAATGG TGAAGTGGAT 
GATCTGAAGC AACTTTTGGA TCAGAAAATT CAGTCTCTGG AGGATCGTCT GGGTAGCAGT 
CTCCTAGACA TTGCTAATGG CACTGACGTA AAATTCATCA ATCTAGAGTC AGTGTTGCCA 
GGATCTGGCA ATGGCCAATT AACTACTGAA CTTAATCATC TGAAGAATAA AATCCAAGAT 
GTGGAGAATC TTTGTCTGCA GAATCTCCAG TTAGGGTCTC ATGAAAAAGA AAGTACTCCT 
CCCAGTAAGT ATGATGATCA GAGGATGGAT ACCTCTGCAC TTCTAAAGGC TCTGAATGAT 
ACAATGCATA GGAAGTTCAA AGAAACTGAA CTTAATATTC AGAAAGTACA ACAAGATTTG 
AGTTTGCTCT TTTCTCATCT AAATGACACT GACAAAGATG TGAAAAATCT TCAGGGTGGG 
TTGAGCAACT GCCAAGAGCA ACTTGCGGAT GCCATTTCTA CATGCAGGCA GGCAGAAGAT 
AGTGTTTCCA GGAAGCTGGA TGAGCTAGAT AGGACCCCTG AAGAGCCCAC TTCTTCCCAG 
GCCCCAGCGG GTAATTGCTG CAGTCATCTG GAGGTGAGGC TACAGGGGCT ACAAAAGCAA 
ATCCTTTCCG ACCTGGACAT CTGTAAGGAA AGCACTCAGG GAGTCCAGAG GGAAGTCATG 
GTTGTTGGAG GAAGAGTGTC TAATGTTGAA AAAGCATGTG CCAAACTGGA TTCCATCTCT 
GGGAGTCTAC ACAGGATCAA GGAAGGATTG AACAAGCATG TCAACAACCT GTGGAACTGT 
GTCAGTCAGA TGAATGGCAC GATTGTAACC CATTCCAAAG ACATTTCTGG CCTCAAAAAT 
TCTGTCCAGC AGATCTATGG TCGTGTCTTC CAAATTACCA CAGACCTTCA GGATCTGGTG 
AAAGGTCAAC CAGCAGGATC GGAAGCCCCA TCAGAAATAC CACAGTTAAT TCCACAACAA 
CCAGAAAGCA CCCCTGAACC ATCACAAATG GAAGTTCCTG GAGAATCATC ACATCCAAAA 
ACACCCCAGC AGCCTCCAGA ATCAAAAGGC CCCCTCCCTC CCAAGACTCC TGGGAAACCC 
TTCCAGCCAG GAATACCTCA GCAGCCTTCA CAATCAAAGA CTCCTCCACA GCCATCACCT 
CCCAAGCACA TGCCATCCCA TTCAGAGTTA CCTGGGTGGA CAGGTTTGCC TTTCCTACCA 
GGATCTACAG GGGTCATCAT GGAGACTGGG GAAGCTGGAC CTCCTGGGAA AATGGTGATG 
TCAGGGAGAG GCTTGCCCAA TGGTGTTGAT GGCCAGGACA GTCGGTGGCC CATCCCAAGC 
TCTGAAGGAT ATGCTGGAGC CCCAGGATAC CCTAAGCGGC CATCTCCTGT GGATTCACAA 
GGGATTCCTA CCGCCTCCCT GGTGTCCTTT TCTGCCGGTC TGACTCAGAA ACCTTTTCCG 
AGTGAGGCGG GTGTGGTTCT CTTCAACAAA GTACTTGTGA ATGATGGAGA ATTTTACGAC 
CCTAACACTG GGATCTTTAC TGCCCCATAT GATGGTCGAT ATCTGATCAC TGCCATTCTC 
ACTCCTGAGA GAGATGAATA TGTTGAAGCT GTCTTGTCGG TCTCTAATGC CAGCGTGGCC 
CAGCTGCACA CAGCCGGATA CAGAAGAGAA CTCTTGGAAT ATCACAGACC CCGAGGGGGG 
TTACATACCT GTGGTGGAAC AGGGACTTTC CATCTCATCG TCCACTTGAA AGCAGGAGAT 
GAGGTGAACA TTGTGGTGAC TGGGGGCAGG CTGGCCCACA CAGACTTTGA TGAAATGTAC 
TCCACATTTA GTGGGGTCTT CCTCTATCCT TTCCTTTCCC ACATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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EMILIN2 cDNA ORF clone, Sarcophilus harrisii(Tasmanian devil)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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