DENND4A cDNA ORF clone, Saccoglossus kowalevskii

Gene
Clones
gRNAs

Summary

Gene Symbol	DENND4A
Entrez Gene ID	100366358
Full Name	DENN/MADD domain containing 4A
Gene Type	protein-coding
Organism	Saccoglossus kowalevskii

mRNA and Protein(s)

mRNA	Protein	Name
XM_002735520.2	XP_002735566.2	C-myc promoter-binding protein

Pathways from BioSystems
Gene Ontology

The following DENND4A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DENND4A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSg05952	XM_002735520.2 Latest version!	Saccoglossus kowalevskii DENN/MADD domain containing 4A (DENND4A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DENND4A ( XM_002735520.2 ) cDNA ORF clone, Saccoglossus kowalevskii -> XP_002735566.2 Saccoglossus kowalevskii DENN/MADD domain containing 4A (DENND4A), mRNA.

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CloneID	OSg05952
Clone ID Related Accession (Same CDS sequence)	XM_002735520.2
Accession Version	XM_002735520.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5955bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1392566400000
Organism	Saccoglossus kowalevskii
Product	C-myc promoter-binding protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003129358.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 18, 2014 this sequence version replaced XM_002735520.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Saccoglossus kowalevskii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTACA ATATGGATGA GAGAGGTCCC AATCGCATTG CAGATTACTG TGTTTTGGCT 
GGACTCAAAG ATACTTCCAG CTTGTTGGAA TATGACCTAC AAAATGAGAG CGGTTTAAAA 
GTGTCCAAGC CCAAAGACCC TGTAGTGGAT ATTGCAGTTG TTATCAAAAG TCAAGGCGAA 
CGAGTTCCAC AAGGTTTTGA ATGTGTGGAG ACAACCCCAA CAGGGTTTTC TGCTGACTTG 
AATCATGGTA GTCTACGCAG TCCAGCCGTC TATCTTTGTT TTCGTCGTGG CACAGACAAA 
CCACCTCTTA CTGATATTGG TATTTTATAC GAAGGTAAAG AGCGAGTGAT GCCAGGTTGT 
GAGATCGTGA GCACAACGCC ATGTGGTCGC CCCGCTAATG TCAACAATAG AACAGGGATG 
GGCAACCAAC GAATTTATAT AACATACAGA AGATCATCAG AGACGGCTGC TCATAACTCT 
CTAGCAGTTA CTGATATCTG TGTTATACTT ACTAATAAGA ATGAGATGCC GCCACACGCA 
TTCTGTATGA TAGATAAGAA TTTGAACAAA GGCATGACAG GTTCTGATGT CTACTTGTGT 
TATAAGAAAT CCATGACACG CGCCAACGCA ATATATTACA AAGCAGGCAT TTTAAGTCGA 
TATCCAGAGG AAGATAACCA AAACTTCTCA TTGCCAGAAG AGGTTCCATT GTTTTGTCTG 
CCTATGGGAG CCACTATAGA GTGCTGGAAA GCTAAAAGAA TGCGTCCACT GCCAGTCTTT 
TCTACATTTG TTCTCACAGA TGCTAAAGGA GTTAAGGTAT ATGGTGCTGC AGTTACTTTT 
TATGAAGCGT ATTCTGAAGA TTTACTGACT GATGAGCAAC GACAGAAGCT GGGCATGCAA 
CCGAAGGGCA AAGAAGATCC CTACAAACTG TTACGAACCC TCCATACCAA TAAATGTATT 
TGTTTGTTAT CGCAGTGGCC ATTCTTTGAG GCATTTAAAA AATTCTTATC CTATTTATAT 
AGGTTATCAG TATCTGGACC CCATTTGGTA CCAATTGAAA GGCATATTTC ACATTTTATG 
TATGAAATAC CATTTCCAAC ACAAGAAAGA CCCAGAATAA TGTTACCACT TGCACATGAT 
GCACTCTATT TGACCCAGCC GCAAACCTCT CCATTACCGC TCAGTGGCGC ATCGTTTACA 
GCTCTTCTAA GGAACTTGGG TCCTGAAAAC TGTCTGACTT TATTAGTATT TGCTGTCACT 
GAACAAAAAA TATTGTTGCA CTCACTTCGT CCAGCAGTAT TGACAAGTGT TGCCGAAGCT 
GTTTCTACAA TGATATTTCC TTTTACATGG CAATGTCCAC ACATACCACT GTGTCCGCTT 
GGCCTGTCTG ATGTCCTCAG TGCACCATTA CCGTTCATTG TGGGCATGGA TTCCCGGTAC 
TTTGATATGT ATGAGCCACC TGCAGATGTT GTATGTGTAG ATCTAGATAC AAATACAATA 
TCACAAAATG ATGAGAGGAA GTTCATGAGC TCAAAGATGT TACCTAAGAG ACCAGCTAAG 
CAATTAAAGA ATACGTTAAC TCGTCTTGCC ACTGAGATTT ACACCAATCC TGTGTATAGC 
GAGAATGTTG ATGACTTAGC AGTGGAAATG GCACCATTAC ATCACGATTT CACGAAAAAA 
CGAAAACAGC TTTCCATGGA ACTTTCCATC CAGGAAGCTT TTCTTCGTTT TATGGCTACG 
TTGTTTAAAG GATATAGACA GTATTTACTA CCAATTACAG ATAAACCGAC AGAACAGACG 
ACGGACCCAA GTTCATTATT TGATCTACAA GGATTTTTGA AAAGTCGTGA TAGAGCTAAC 
TATAAACTGT TCAGCCAGCT GATGAAAACT CAGTCGTTTA TACGATTTAT AGAAGAAAGA 
TCGTTTGTGT CGAACAATGA TGCAAGTTTA GCTTTTTTTG ATGAGTGTGC AGAGAAGGTG 
GATGCTAAAT GTGAAATAGA GACAAAACTG ATGGAATTAG AAGAATCTTT TGAAAGTGCA 
AGAACTGTGT TTGTTACACC ACCGGAACCA ATTAATAATG AAAAATACAG CTATACGCAT 
TTTCCAGTAC TGAAAAAGGA GTTGTTTTCA CCGGCAAACA TGCATACCTT GGCCATTAGT 
AAGTCACTTA AAAGCACTGT TCCTAGTAGT CCCGTGGCAA GAAGAACCAA ACAAGAAGCC 
AAGTTATCTC AGATTAGTGC TAAAGAAAAT GCAAAGAATC CCAAACTGTG GGCAAAGTGT 
TTACTGGGGC TATGTTTCAG CGCCTGGTTT ATACATCTGC CAGCCTATGT CAAGTCAACC 
CATTCTAAAA CTAAAGCTCT TCGAACTGCA TTTGAAGCAC TGGTGAAAAT GGGTACATGT 
AAATTAGAGC CCCCTGATGA GATATGTTAC AGAGTACTAA TGCAACTCTG TGGACAGTAT 
GGCCTGCCAG TCTTAGCTGT CAAAGTGCTG TTTGAAATGA AAAAAAGTGG AATTCAACCA 
AATGCAATAA CATACGGTTT CTATAACAGA GCTGTTTTGG AAAATGAATG GCCAGCTACT 
ACTACTAGTG GCTATATACT GTGGACAAAG CTGCGTAATG TTATTTTGGC AGTAGCGCAG 
TTCCGCCGTT GTGTAAAAAC CGACATGGAT AAAATCAGTT TGTGCAGCAG TGAGAGCAGT 
TATGCAGATG GCGTTGGAAG AACTAGCAGT GAAGATAATA AAGAATTTGT CAATGATCTT 
GTTAAATTAG ATTCACCGGT AGCAGTGGAG GACAAGTCGA GCACAGGTGG ACACTCTGAT 
ATGGGTTATA GTTCTATGTC AATTGACGAT GCCAGTCGTA GCAACAGCTT GACAACAATG 
TCTCCGTCTT CACCGCAGAA ATCTACCAAT GATGCCCTGG GTACTAAGAC AAAGAAAAAA 
ACGAGTAAAT CGAATGTCGC TGTCACAGAG AGTGGACCAG TAGACATGAA CAGCTCTGAT 
AGTTTCAGGC CACGGGTCAG CAGTATAGTT CGCAACTCTG TCGGCAGTTT TGGCAGTACC 
AGTAGCATCG GAACATTACG TGGCAGTACA AGCAGCACAT CAGGGTTGTT ATTAACAAGT 
CAGAATTCCC TGGAGGAAAG CAGTGTGTTT GATAGCACAC CTGAATCTAA TGATGCTAAG 
GCCCGTAAAA GACACAAAAG TGCTGGAGAT CACAGCAGAC TGTCCCGTAA AATGTTGCGT 
AATCGTAATT TCAGCGGTGA TAGTACTGCC ACACTGACGT TAAACTTAAG AGATAATAGT 
AGTTTTGAAA GTGATCCTGG AATGATTAAA GCCGATTCAT TTGGAAGTGA TGCACGCATA 
ATTAAAGCAC TCAGAAAGTC AGATCCTAAA CTGAATTCTT CCCGACAAAA AAAGCAACGT 
TCGAAAAGAA AAACTAAATT TGACTTGGGT GGTGATGATA TTGATGATGA TATTGATGCT 
GATGAAGAGG AAGATAATGA GGAGGAGGAG GAGGAGGAGG AAGAAGAGGA AATCACGCCG 
TCACATATTA GAAGGTGTAA CTCAGAAGGT CAAAAGCCAA TGAGATTGAG GATTATCATG 
GAACGTGATA AAAGACAAAA AGAGGAGCAA ATGAAGGCAA AAGAAAAGCG AACAGACAGT 
GACACTTCTG AACCTGAGAC AAAACAGAAG TCTGAAAACG GCGAGATGCC AAGGGTAGAT 
TCAACTTTAG ACACAATTCT GCTTGAATTT GATGATTTGG CACCAACTAA AGATAAAAGT 
GGGGATTCAA GTGATGGCAC TGAAACAGAT GAAGGAAAAT TAAATCCTGA TGAAGATGAA 
CGGTCAAGAA GCAGTTCTAT ATCTATTCAG CAGAAACCAT TTCGAGAAGA AACTAACATT 
CAGATTATTT CGTCAAGTTT TGAAGAACAC ACTGATAGTA AGTGGCAGGC TGATGATCAG 
GATATACCAC ATTCATTGCC CAATCCTACA TTTATCCCAG AGAGCCCATC AGAAAGGATA 
GGTATGAAAA CTCCACCGCG AAACTTCAGT AGCCATTTCC TACAAGGACT GATACAACAG 
GTGACCAGTC CGCCAACAAC AACATGGTTC GGGAGCAGCC CATTCAGTAA AAAAGGCAGT 
GATGCTGGTG GGTCAACATC AAAATTAGAC GCCATCAAGG CAGCAGCCAG CGCCATGGCA 
AATAAATTTG GATATGCTAA ACAGAAATCA ACATCGTCAA CTGCAACAAA GTCGTCAACA 
TCTAGTATTC CACAAGATGT AGAAGAAACT GGCAGTCTTT TATCATTCAA AGAAGACTGT 
GATTCCAAAC ATGGTACGTG GGTACCCCAT AGTAAGAGTG ACACTAATCT AGTAGGCACT 
TTGTTAATGG ATGAATCACC TTCTACTTTT GCAGATTTAG TTACAGGGAG TACACCATCA 
CTGAATGCCC CCTTAGCTGA CCAATCGGAT GGGAGTAATA CCAAGCTGAA TGCCAGTACT 
CAGAGTCTTT TTACGTATGC CATGGAAGTA AATATTACAA GCTGTATGCG ATGTAATTCA 
TGTAGCTGTG TGTTGTACGA TGAAGCCATC ATGTCAGGCT GGTCAGCGGA TGATTCAAAT 
CTCAATACTA CGTGTTGTTA TTGTGGTTCC CTATTTGTGC CATTCCTGGA CATCACGATG 
AAAGATTTCA GAACCCCTGG GATTGGGCGA GCTATAATAT CTCCTAGCCA ATCAGCAGAA 
AGTATGTTGA GCATACAATC GGCACCATCT GCTCAGCCAC TCCCACCGCC ACTAACCGGC 
GATGAACAAT CAAGTGTTGA GAATTTGATG TTTCTGAATG AGAACGATGA AGATGAAATT 
GACGGAGACA ATAACGGTAC TCCTAGTGAA GTGAAATCAA AAGAAAAGAA TAGTCCAAGG 
CAATTAAAGT ATGTCCGGAA CATTAGCAGC CCTGGTGATT TACATATGTT GACACTGATG 
GTGGTAGTCA TTCTAAATCC GAACTCGGGT TCAGTAAGAT CTGCGCATGA ACGGCGGAGA 
TGCACCAGTG AGTGTTTAAC ATCCGGGACG ACCCAACCAC TGAGAATAAA CGATAAAAGA 
CATAGATACA GTGCACCGCC TCAAGATGGT TCAATAGGAG GAGATGAAGA GGTGTTATCT 
TCCAGTATTT CATCCAGTAT ACAAGGCTCA CGAGAATACT TGCCACCACA AAGGAAATCC 
ACCAATCCTG ACCCTGTATC TGTGCCGTAT CTCAGTCCGC TGGTACTTCG CAAGGAGATG 
GAAAATGTTA TCGATAACGA AGGTGACATC TGCTTTCTTT CGTCTGACTT TGTAGATGAA 
CATCCAATCA TCTTTTGGAA TTTGGTTTGG GTTTTTAAAA GAGTGGAACT CACCAGTAAT 
TTACCAGGAT TACTGTTGTC AGCTAACAGC ATTAATAAAG AGCATCACAT TCAATCAAGT 
AACCAGTGGT TGTCTCGTGA CAGTCGACAC GTCTTGATCA ATGCTTTGTG GGATAATCCC 
AAATATCAAG AATCCAGACC ACCAATGTAC ACTGTGTGGA ATGCAATACA GCAAGGATCT 
TCTGTAGATA CACTTATCAA TGAACAACAT GCATTTTCTG GAAAATTTAT GCAGTCTATC 
GTGCAGGCCA TCCAAGGCAA TGATGTAGGG ACACCGATAC GGTGGTTGTT AGAGCAATAC 
AGGAAACGTC AACCTGATAA AAGACATCGT AGCATTTACA GAGATATATT ATTTTTAGCA 
TTAATTGCTT GTGGTCGTCA AAACATAGAT GTTGATGCTT TCGATCGTGA GTACGACATA 
GCATTCGATA AATTACTTGC AGAGTACTGG GGACCAAGTC AGTTGGAACA CTTGCAAGAA 
GATGACAAGC CTCCCAACGA TGCGATGTTC TGGTGTCGTA AAATTTTTGG AGAAGTTGCT 
GAATTAAGTC TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002735566.2
CDS	158..6112
Translation	MNYNMDERGPNRIADYCVLAGLKDTSSLLEYDLQNESGLKVSKPKDPVVDIAVVIKSQGERVPQGFECVETTPTGFSADLNHGSLRSPAVYLCFRRGTDKPPLTDIGILYEGKERVMPGCEIVSTTPCGRPANVNNRTGMGNQRIYITYRRSSETAAHNSLAVTDICVILTNKNEMPPHAFCMIDKNLNKGMTGSDVYLCYKKSMTRANAIYYKAGILSRYPEEDNQNFSLPEEVPLFCLPMGATIECWKAKRMRPLPVFSTFVLTDAKGVKVYGAAVTFYEAYSEDLLTDEQRQKLGMQPKGKEDPYKLLRTLHTNKCICLLSQWPFFEAFKKFLSYLYRLSVSGPHLVPIERHISHFMYEIPFPTQERPRIMLPLAHDALYLTQPQTSPLPLSGASFTALLRNLGPENCLTLLVFAVTEQKILLHSLRPAVLTSVAEAVSTMIFPFTWQCPHIPLCPLGLSDVLSAPLPFIVGMDSRYFDMYEPPADVVCVDLDTNTISQNDERKFMSSKMLPKRPAKQLKNTLTRLATEIYTNPVYSENVDDLAVEMAPLHHDFTKKRKQLSMELSIQEAFLRFMATLFKGYRQYLLPITDKPTEQTTDPSSLFDLQGFLKSRDRANYKLFSQLMKTQSFIRFIEERSFVSNNDASLAFFDECAEKVDAKCEIETKLMELEESFESARTVFVTPPEPINNEKYSYTHFPVLKKELFSPANMHTLAISKSLKSTVPSSPVARRTKQEAKLSQISAKENAKNPKLWAKCLLGLCFSAWFIHLPAYVKSTHSKTKALRTAFEALVKMGTCKLEPPDEICYRVLMQLCGQYGLPVLAVKVLFEMKKSGIQPNAITYGFYNRAVLENEWPATTTSGYILWTKLRNVILAVAQFRRCVKTDMDKISLCSSESSYADGVGRTSSEDNKEFVNDLVKLDSPVAVEDKSSTGGHSDMGYSSMSIDDASRSNSLTTMSPSSPQKSTNDALGTKTKKKTSKSNVAVTESGPVDMNSSDSFRPRVSSIVRNSVGSFGSTSSIGTLRGSTSSTSGLLLTSQNSLEESSVFDSTPESNDAKARKRHKSAGDHSRLSRKMLRNRNFSGDSTATLTLNLRDNSSFESDPGMIKADSFGSDARIIKALRKSDPKLNSSRQKKQRSKRKTKFDLGGDDIDDDIDADEEEDNEEEEEEEEEEEITPSHIRRCNSEGQKPMRLRIIMERDKRQKEEQMKAKEKRTDSDTSEPETKQKSENGEMPRVDSTLDTILLEFDDLAPTKDKSGDSSDGTETDEGKLNPDEDERSRSSSISIQQKPFREETNIQIISSSFEEHTDSKWQADDQDIPHSLPNPTFIPESPSERIGMKTPPRNFSSHFLQGLIQQVTSPPTTTWFGSSPFSKKGSDAGGSTSKLDAIKAAASAMANKFGYAKQKSTSSTATKSSTSSIPQDVEETGSLLSFKEDCDSKHGTWVPHSKSDTNLVGTLLMDESPSTFADLVTGSTPSLNAPLADQSDGSNTKLNASTQSLFTYAMEVNITSCMRCNSCSCVLYDEAIMSGWSADDSNLNTTCCYCGSLFVPFLDITMKDFRTPGIGRAIISPSQSAESMLSIQSAPSAQPLPPPLTGDEQSSVENLMFLNENDEDEIDGDNNGTPSEVKSKEKNSPRQLKYVRNISSPGDLHMLTLMVVVILNPNSGSVRSAHERRRCTSECLTSGTTQPLRINDKRHRYSAPPQDGSIGGDEEVLSSSISSSIQGSREYLPPQRKSTNPDPVSVPYLSPLVLRKEMENVIDNEGDICFLSSDFVDEHPIIFWNLVWVFKRVELTSNLPGLLLSANSINKEHHIQSSNQWLSRDSRHVLINALWDNPKYQESRPPMYTVWNAIQQGSSVDTLINEQHAFSGKFMQSIVQAIQGNDVGTPIRWLLEQYRKRQPDKRHRSIYRDILFLALIACGRQNIDVDAFDREYDIAFDKLLAEYWGPSQLEHLQEDDKPPNDAMFWCRKIFGEVAELSL

Target ORF information:

RefSeq Version	XM_002735520.2
Organism	Saccoglossus kowalevskii
Definition	Saccoglossus kowalevskii DENN/MADD domain containing 4A (DENND4A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002735520.2

ORF Insert Sequence:

ATGAATTACA ATATGGATGA GAGAGGTCCC AATCGCATTG CAGATTACTG TGTTTTGGCT 
GGACTCAAAG ATACTTCCAG CTTGTTGGAA TATGACCTAC AAAATGAGAG CGGTTTAAAA 
GTGTCCAAGC CCAAAGACCC TGTAGTGGAT ATTGCAGTTG TTATCAAAAG TCAAGGCGAA 
CGAGTTCCAC AAGGTTTTGA ATGTGTGGAG ACAACCCCAA CAGGGTTTTC TGCTGACTTG 
AATCATGGTA GTCTACGCAG TCCAGCCGTC TATCTTTGTT TTCGTCGTGG CACAGACAAA 
CCACCTCTTA CTGATATTGG TATTTTATAC GAAGGTAAAG AGCGAGTGAT GCCAGGTTGT 
GAGATCGTGA GCACAACGCC ATGTGGTCGC CCCGCTAATG TCAACAATAG AACAGGGATG 
GGCAACCAAC GAATTTATAT AACATACAGA AGATCATCAG AGACGGCTGC TCATAACTCT 
CTAGCAGTTA CTGATATCTG TGTTATACTT ACTAATAAGA ATGAGATGCC GCCACACGCA 
TTCTGTATGA TAGATAAGAA TTTGAACAAA GGCATGACAG GTTCTGATGT CTACTTGTGT 
TATAAGAAAT CCATGACACG CGCCAACGCA ATATATTACA AAGCAGGCAT TTTAAGTCGA 
TATCCAGAGG AAGATAACCA AAACTTCTCA TTGCCAGAAG AGGTTCCATT GTTTTGTCTG 
CCTATGGGAG CCACTATAGA GTGCTGGAAA GCTAAAAGAA TGCGTCCACT GCCAGTCTTT 
TCTACATTTG TTCTCACAGA TGCTAAAGGA GTTAAGGTAT ATGGTGCTGC AGTTACTTTT 
TATGAAGCGT ATTCTGAAGA TTTACTGACT GATGAGCAAC GACAGAAGCT GGGCATGCAA 
CCGAAGGGCA AAGAAGATCC CTACAAACTG TTACGAACCC TCCATACCAA TAAATGTATT 
TGTTTGTTAT CGCAGTGGCC ATTCTTTGAG GCATTTAAAA AATTCTTATC CTATTTATAT 
AGGTTATCAG TATCTGGACC CCATTTGGTA CCAATTGAAA GGCATATTTC ACATTTTATG 
TATGAAATAC CATTTCCAAC ACAAGAAAGA CCCAGAATAA TGTTACCACT TGCACATGAT 
GCACTCTATT TGACCCAGCC GCAAACCTCT CCATTACCGC TCAGTGGCGC ATCGTTTACA 
GCTCTTCTAA GGAACTTGGG TCCTGAAAAC TGTCTGACTT TATTAGTATT TGCTGTCACT 
GAACAAAAAA TATTGTTGCA CTCACTTCGT CCAGCAGTAT TGACAAGTGT TGCCGAAGCT 
GTTTCTACAA TGATATTTCC TTTTACATGG CAATGTCCAC ACATACCACT GTGTCCGCTT 
GGCCTGTCTG ATGTCCTCAG TGCACCATTA CCGTTCATTG TGGGCATGGA TTCCCGGTAC 
TTTGATATGT ATGAGCCACC TGCAGATGTT GTATGTGTAG ATCTAGATAC AAATACAATA 
TCACAAAATG ATGAGAGGAA GTTCATGAGC TCAAAGATGT TACCTAAGAG ACCAGCTAAG 
CAATTAAAGA ATACGTTAAC TCGTCTTGCC ACTGAGATTT ACACCAATCC TGTGTATAGC 
GAGAATGTTG ATGACTTAGC AGTGGAAATG GCACCATTAC ATCACGATTT CACGAAAAAA 
CGAAAACAGC TTTCCATGGA ACTTTCCATC CAGGAAGCTT TTCTTCGTTT TATGGCTACG 
TTGTTTAAAG GATATAGACA GTATTTACTA CCAATTACAG ATAAACCGAC AGAACAGACG 
ACGGACCCAA GTTCATTATT TGATCTACAA GGATTTTTGA AAAGTCGTGA TAGAGCTAAC 
TATAAACTGT TCAGCCAGCT GATGAAAACT CAGTCGTTTA TACGATTTAT AGAAGAAAGA 
TCGTTTGTGT CGAACAATGA TGCAAGTTTA GCTTTTTTTG ATGAGTGTGC AGAGAAGGTG 
GATGCTAAAT GTGAAATAGA GACAAAACTG ATGGAATTAG AAGAATCTTT TGAAAGTGCA 
AGAACTGTGT TTGTTACACC ACCGGAACCA ATTAATAATG AAAAATACAG CTATACGCAT 
TTTCCAGTAC TGAAAAAGGA GTTGTTTTCA CCGGCAAACA TGCATACCTT GGCCATTAGT 
AAGTCACTTA AAAGCACTGT TCCTAGTAGT CCCGTGGCAA GAAGAACCAA ACAAGAAGCC 
AAGTTATCTC AGATTAGTGC TAAAGAAAAT GCAAAGAATC CCAAACTGTG GGCAAAGTGT 
TTACTGGGGC TATGTTTCAG CGCCTGGTTT ATACATCTGC CAGCCTATGT CAAGTCAACC 
CATTCTAAAA CTAAAGCTCT TCGAACTGCA TTTGAAGCAC TGGTGAAAAT GGGTACATGT 
AAATTAGAGC CCCCTGATGA GATATGTTAC AGAGTACTAA TGCAACTCTG TGGACAGTAT 
GGCCTGCCAG TCTTAGCTGT CAAAGTGCTG TTTGAAATGA AAAAAAGTGG AATTCAACCA 
AATGCAATAA CATACGGTTT CTATAACAGA GCTGTTTTGG AAAATGAATG GCCAGCTACT 
ACTACTAGTG GCTATATACT GTGGACAAAG CTGCGTAATG TTATTTTGGC AGTAGCGCAG 
TTCCGCCGTT GTGTAAAAAC CGACATGGAT AAAATCAGTT TGTGCAGCAG TGAGAGCAGT 
TATGCAGATG GCGTTGGAAG AACTAGCAGT GAAGATAATA AAGAATTTGT CAATGATCTT 
GTTAAATTAG ATTCACCGGT AGCAGTGGAG GACAAGTCGA GCACAGGTGG ACACTCTGAT 
ATGGGTTATA GTTCTATGTC AATTGACGAT GCCAGTCGTA GCAACAGCTT GACAACAATG 
TCTCCGTCTT CACCGCAGAA ATCTACCAAT GATGCCCTGG GTACTAAGAC AAAGAAAAAA 
ACGAGTAAAT CGAATGTCGC TGTCACAGAG AGTGGACCAG TAGACATGAA CAGCTCTGAT 
AGTTTCAGGC CACGGGTCAG CAGTATAGTT CGCAACTCTG TCGGCAGTTT TGGCAGTACC 
AGTAGCATCG GAACATTACG TGGCAGTACA AGCAGCACAT CAGGGTTGTT ATTAACAAGT 
CAGAATTCCC TGGAGGAAAG CAGTGTGTTT GATAGCACAC CTGAATCTAA TGATGCTAAG 
GCCCGTAAAA GACACAAAAG TGCTGGAGAT CACAGCAGAC TGTCCCGTAA AATGTTGCGT 
AATCGTAATT TCAGCGGTGA TAGTACTGCC ACACTGACGT TAAACTTAAG AGATAATAGT 
AGTTTTGAAA GTGATCCTGG AATGATTAAA GCCGATTCAT TTGGAAGTGA TGCACGCATA 
ATTAAAGCAC TCAGAAAGTC AGATCCTAAA CTGAATTCTT CCCGACAAAA AAAGCAACGT 
TCGAAAAGAA AAACTAAATT TGACTTGGGT GGTGATGATA TTGATGATGA TATTGATGCT 
GATGAAGAGG AAGATAATGA GGAGGAGGAG GAGGAGGAGG AAGAAGAGGA AATCACGCCG 
TCACATATTA GAAGGTGTAA CTCAGAAGGT CAAAAGCCAA TGAGATTGAG GATTATCATG 
GAACGTGATA AAAGACAAAA AGAGGAGCAA ATGAAGGCAA AAGAAAAGCG AACAGACAGT 
GACACTTCTG AACCTGAGAC AAAACAGAAG TCTGAAAACG GCGAGATGCC AAGGGTAGAT 
TCAACTTTAG ACACAATTCT GCTTGAATTT GATGATTTGG CACCAACTAA AGATAAAAGT 
GGGGATTCAA GTGATGGCAC TGAAACAGAT GAAGGAAAAT TAAATCCTGA TGAAGATGAA 
CGGTCAAGAA GCAGTTCTAT ATCTATTCAG CAGAAACCAT TTCGAGAAGA AACTAACATT 
CAGATTATTT CGTCAAGTTT TGAAGAACAC ACTGATAGTA AGTGGCAGGC TGATGATCAG 
GATATACCAC ATTCATTGCC CAATCCTACA TTTATCCCAG AGAGCCCATC AGAAAGGATA 
GGTATGAAAA CTCCACCGCG AAACTTCAGT AGCCATTTCC TACAAGGACT GATACAACAG 
GTGACCAGTC CGCCAACAAC AACATGGTTC GGGAGCAGCC CATTCAGTAA AAAAGGCAGT 
GATGCTGGTG GGTCAACATC AAAATTAGAC GCCATCAAGG CAGCAGCCAG CGCCATGGCA 
AATAAATTTG GATATGCTAA ACAGAAATCA ACATCGTCAA CTGCAACAAA GTCGTCAACA 
TCTAGTATTC CACAAGATGT AGAAGAAACT GGCAGTCTTT TATCATTCAA AGAAGACTGT 
GATTCCAAAC ATGGTACGTG GGTACCCCAT AGTAAGAGTG ACACTAATCT AGTAGGCACT 
TTGTTAATGG ATGAATCACC TTCTACTTTT GCAGATTTAG TTACAGGGAG TACACCATCA 
CTGAATGCCC CCTTAGCTGA CCAATCGGAT GGGAGTAATA CCAAGCTGAA TGCCAGTACT 
CAGAGTCTTT TTACGTATGC CATGGAAGTA AATATTACAA GCTGTATGCG ATGTAATTCA 
TGTAGCTGTG TGTTGTACGA TGAAGCCATC ATGTCAGGCT GGTCAGCGGA TGATTCAAAT 
CTCAATACTA CGTGTTGTTA TTGTGGTTCC CTATTTGTGC CATTCCTGGA CATCACGATG 
AAAGATTTCA GAACCCCTGG GATTGGGCGA GCTATAATAT CTCCTAGCCA ATCAGCAGAA 
AGTATGTTGA GCATACAATC GGCACCATCT GCTCAGCCAC TCCCACCGCC ACTAACCGGC 
GATGAACAAT CAAGTGTTGA GAATTTGATG TTTCTGAATG AGAACGATGA AGATGAAATT 
GACGGAGACA ATAACGGTAC TCCTAGTGAA GTGAAATCAA AAGAAAAGAA TAGTCCAAGG 
CAATTAAAGT ATGTCCGGAA CATTAGCAGC CCTGGTGATT TACATATGTT GACACTGATG 
GTGGTAGTCA TTCTAAATCC GAACTCGGGT TCAGTAAGAT CTGCGCATGA ACGGCGGAGA 
TGCACCAGTG AGTGTTTAAC ATCCGGGACG ACCCAACCAC TGAGAATAAA CGATAAAAGA 
CATAGATACA GTGCACCGCC TCAAGATGGT TCAATAGGAG GAGATGAAGA GGTGTTATCT 
TCCAGTATTT CATCCAGTAT ACAAGGCTCA CGAGAATACT TGCCACCACA AAGGAAATCC 
ACCAATCCTG ACCCTGTATC TGTGCCGTAT CTCAGTCCGC TGGTACTTCG CAAGGAGATG 
GAAAATGTTA TCGATAACGA AGGTGACATC TGCTTTCTTT CGTCTGACTT TGTAGATGAA 
CATCCAATCA TCTTTTGGAA TTTGGTTTGG GTTTTTAAAA GAGTGGAACT CACCAGTAAT 
TTACCAGGAT TACTGTTGTC AGCTAACAGC ATTAATAAAG AGCATCACAT TCAATCAAGT 
AACCAGTGGT TGTCTCGTGA CAGTCGACAC GTCTTGATCA ATGCTTTGTG GGATAATCCC 
AAATATCAAG AATCCAGACC ACCAATGTAC ACTGTGTGGA ATGCAATACA GCAAGGATCT 
TCTGTAGATA CACTTATCAA TGAACAACAT GCATTTTCTG GAAAATTTAT GCAGTCTATC 
GTGCAGGCCA TCCAAGGCAA TGATGTAGGG ACACCGATAC GGTGGTTGTT AGAGCAATAC 
AGGAAACGTC AACCTGATAA AAGACATCGT AGCATTTACA GAGATATATT ATTTTTAGCA 
TTAATTGCTT GTGGTCGTCA AAACATAGAT GTTGATGCTT TCGATCGTGA GTACGACATA 
GCATTCGATA AATTACTTGC AGAGTACTGG GGACCAAGTC AGTTGGAACA CTTGCAAGAA 
GATGACAAGC CTCCCAACGA TGCGATGTTC TGGTGTCGTA AAATTTTTGG AGAAGTTGCT 
GAATTAAGTC TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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DENND4A cDNA ORF clone, Saccoglossus kowalevskii

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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