LOC8282375 cDNA ORF clone, Ricinus communis(castor bean)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC8282375
Entrez Gene ID	8282375
Full Name	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
Gene Type	protein-coding
Organism	Ricinus communis(castor bean)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002525663.3	XP_002525709.1	5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase
XM_025158541.1	XP_025014309.1	5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase
XM_002525663.2	XP_002525709.1	5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase

Pathways from BioSystems
Gene Ontology

The following LOC8282375 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC8282375 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORb21530	XM_002525663.3 Latest version!	Ricinus communis 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (LOC8282375), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ORb21530	XM_025158541.1 Latest version!	Ricinus communis 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (LOC8282375), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
ORb21530	XM_002525663.2	Ricinus communis 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (LOC8282375), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC8282375 ( XM_002525663.3 ) cDNA ORF clone, Ricinus communis(castor bean) -> XP_002525709.1 Ricinus communis 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (LOC8282375), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORb21530
Clone ID Related Accession (Same CDS sequence)	XM_002525663.3 , XM_025158541.1 , XM_002525663.2
Accession Version	XM_002525663.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526918400000
Organism	Ricinus communis(castor bean)
Product	5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002994473.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 23, 2018 this sequence version replaced XM_002525663.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ricinus communis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCTC ACATTGTTGG ATATCCTCGT ATGGGCCCTA AGAGAGAGCT CAAGTTTGCA 
TTGGAATCTT TTTGGGATGG AAAGAGCAGT GCTCAGGACT TGGAAAAGGT GGCATCTGAT 
CTCAGGGAAT CCATCTGGAA GCAGATGGCT GCTGCTGGGA TCAAACATAT CCCCAGCAAT 
ACTTTCTCTT ACTACGATCA GGTGCTTGAT ACCACAGCAA TGCTTGGAGC TGTTCCACCT 
AGATATGGCT GGAATGGTGG TGAGATTGGA TTCGATACAT ACTTCTCCAT GGCTAGAGGA 
AACGCCTCTG TGCCCGCTAT GGAAATGACC AAGTGGTTTG ACACCAATTA CCATTTCATT 
GTCCCTGAAT TGGGACCTGA TGTCAACTTC TCTTACGCTT CTCACAAAGC AGTGACCGAA 
TACAAGGAGG CTAAGGCGCA TGGAGTAGAC ACCGTTCCTG TTCTTGTTGG CCCTGTCTCT 
TATTTGTTAC TTTCTAAACC TGCCAAGGGT GTTGAGAAAA CCTTTTCTCT TCTCTCCCTT 
CTTGGAAAGA TCCTTCCAGT CTACAAGGAG GTTATTTCTG AGCTTAAAGC AGCTGGTGCA 
TCCTGGATTC AGTTTGATGA GCCTACACTT GTTTTGGATC TTGATTCTCA CAAACTGCAA 
GCATTTACTG ATGCCTACTC TGAGTTGGAG TCGACCCTAT CTGGCTTGAA TGTTCTCATT 
GAGACATACT TTGCTGACAT CCCAGCTGAG GCATTCAAGA CACTCACATC TCTTAAGGGC 
GTCACTGCAT ATGGTTTTGA TTTGGTTCGT GGAACTAAGA CCCTTGACTT GATCAAGAGT 
GAATTCCCCA AGGGCAAATA CCTATTTGCT GGAGTAGTTG ATGGAAGGAA TATTTGGGCC 
AATGATCTTG CTGCTTCTCT TACCATCTTG CATGAACTTG AAGGCATTGT GGGCAAGGAC 
AAACTCGTAG TCTCCACCTC TTCTTCACTT CTTCACACTG CTGTTGATCT CGTGAACGAG 
ACTAAGTTGG ACAAGGAACT AAAATCATGG CTTGCATTTG CTGCCCAGAA AATTGTCGAA 
GTTAATGCAT TGGCCAAGGC TTTGGCTGGT GAAAAAGATG AGGCTTTCTT CTCTGCTAAT 
GCTGCTGCAC AGGCCTCAAG GAAGTCCTCT CCAAGGGTGA CCAATGAGGC TGTTCAAAAG 
GCTGCTGCTG CTTTGAAGGG TTCTGACCAC CGCCGAGCTA CAAATGTTAG TGCCAGACTT 
GATGCCCAAC AGAAAAAGCT TAATCTTCCA ATCCTCCCAA CCACCACAAT TGGGTCCTTC 
CCACAGACCA TAGAACTCAG GAGAGTCCGC CGTGAATACA AGGCCAAAAA GGTCTCTGAG 
GACGATTACG TTAAAGCCAT CAAGGAGGAG ATCAGCAAAG TTGTCAAACT CCAAGAGGAG 
CTTGATATTG ATGTTCTAGT CCATGGAGAG CCTGAGAGAA ACGATATGGT TGAGTACTTT 
GGAGAGCAGT TGTCAGGTTT TGCCTTCACT GTCAATGGAT GGGTGCAATC TTATGGATCT 
CGATGTGTGA AGCCGCCAAT CATCTATGGT GATGTTAGCC GTCCCAACCC AATGACTGTC 
TTCTGGTCCT CGACAGCTCA GAGCATGACT GCCCGCCCAA TGAAGGGAAT GCTTACAGGC 
CCTGTCACCA TTCTCAACTG GTCCTTTGTC AGAAATGACC AGCCAAGATT TGAGACCTGT 
TATCAGATTG CTTTGGCCAT TAAGGATGAA GTGGAGGATC TTGAGAAGGC TGGTATCAAC 
GTTATCCAAA TCGATGAGGC TGCATTAAGA GAGGGTTTGC CTCTTAGGAA GTCCGAGCAA 
GCTTTCTACT TAGATTGGGC AGTGCACTCC TTCAGAATCA CCAATGTTGG AGTCCAGGAC 
ACCACCCAGA TCCACACTCA CATGTGCTAC TCCAACTTCA ATGACATTAT TCACTCAATT 
ATCGACATGG ATGCTGATGT GATCACCATT GAGAACTCCC GTTCTGATGA GAAGCTCCTT 
TCAGTGTTCC GTGAGGGAGT GAAGTATGGA GCTGGCATTG GCCCTGGTGT ATACGATATC 
CACTCTCCAA GAATACCATC AACTGAGGAA ATTGCTGACA GAATTAACAA GATGCTTGCA 
GTTCTTGAAA TGAACATCTT GTGGGTTAAC CCTGACTGCG GTCTCAAGAC TCGCAAGTAT 
TCAGAGGTCA AGCCAGCTCT CAGCAACATG GTCGCCGCAG CCAAGCTCCT CCGCACTCAG 
CTTGCTAGTG CTAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002525709.1
CDS	129..2426
Translation	MASHIVGYPRMGPKRELKFALESFWDGKSSAQDLEKVASDLRESIWKQMAAAGIKHIPSNTFSYYDQVLDTTAMLGAVPPRYGWNGGEIGFDTYFSMARGNASVPAMEMTKWFDTNYHFIVPELGPDVNFSYASHKAVTEYKEAKAHGVDTVPVLVGPVSYLLLSKPAKGVEKTFSLLSLLGKILPVYKEVISELKAAGASWIQFDEPTLVLDLDSHKLQAFTDAYSELESTLSGLNVLIETYFADIPAEAFKTLTSLKGVTAYGFDLVRGTKTLDLIKSEFPKGKYLFAGVVDGRNIWANDLAASLTILHELEGIVGKDKLVVSTSSSLLHTAVDLVNETKLDKELKSWLAFAAQKIVEVNALAKALAGEKDEAFFSANAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRATNVSARLDAQQKKLNLPILPTTTIGSFPQTIELRRVRREYKAKKVSEDDYVKAIKEEISKVVKLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRPNPMTVFWSSTAQSMTARPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEQAFYLDWAVHSFRITNVGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLEMNILWVNPDCGLKTRKYSEVKPALSNMVAAAKLLRTQLASAK

Target ORF information:

RefSeq Version	XM_002525663.3
Organism	Ricinus communis(castor bean)
Definition	Ricinus communis 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (LOC8282375), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002525663.3

ORF Insert Sequence:

ATGGCTTCTC ACATTGTTGG ATATCCTCGT ATGGGCCCTA AGAGAGAGCT CAAGTTTGCA 
TTGGAATCTT TTTGGGATGG AAAGAGCAGT GCTCAGGACT TGGAAAAGGT GGCATCTGAT 
CTCAGGGAAT CCATCTGGAA GCAGATGGCT GCTGCTGGGA TCAAACATAT CCCCAGCAAT 
ACTTTCTCTT ACTACGATCA GGTGCTTGAT ACCACAGCAA TGCTTGGAGC TGTTCCACCT 
AGATATGGCT GGAATGGTGG TGAGATTGGA TTCGATACAT ACTTCTCCAT GGCTAGAGGA 
AACGCCTCTG TGCCCGCTAT GGAAATGACC AAGTGGTTTG ACACCAATTA CCATTTCATT 
GTCCCTGAAT TGGGACCTGA TGTCAACTTC TCTTACGCTT CTCACAAAGC AGTGACCGAA 
TACAAGGAGG CTAAGGCGCA TGGAGTAGAC ACCGTTCCTG TTCTTGTTGG CCCTGTCTCT 
TATTTGTTAC TTTCTAAACC TGCCAAGGGT GTTGAGAAAA CCTTTTCTCT TCTCTCCCTT 
CTTGGAAAGA TCCTTCCAGT CTACAAGGAG GTTATTTCTG AGCTTAAAGC AGCTGGTGCA 
TCCTGGATTC AGTTTGATGA GCCTACACTT GTTTTGGATC TTGATTCTCA CAAACTGCAA 
GCATTTACTG ATGCCTACTC TGAGTTGGAG TCGACCCTAT CTGGCTTGAA TGTTCTCATT 
GAGACATACT TTGCTGACAT CCCAGCTGAG GCATTCAAGA CACTCACATC TCTTAAGGGC 
GTCACTGCAT ATGGTTTTGA TTTGGTTCGT GGAACTAAGA CCCTTGACTT GATCAAGAGT 
GAATTCCCCA AGGGCAAATA CCTATTTGCT GGAGTAGTTG ATGGAAGGAA TATTTGGGCC 
AATGATCTTG CTGCTTCTCT TACCATCTTG CATGAACTTG AAGGCATTGT GGGCAAGGAC 
AAACTCGTAG TCTCCACCTC TTCTTCACTT CTTCACACTG CTGTTGATCT CGTGAACGAG 
ACTAAGTTGG ACAAGGAACT AAAATCATGG CTTGCATTTG CTGCCCAGAA AATTGTCGAA 
GTTAATGCAT TGGCCAAGGC TTTGGCTGGT GAAAAAGATG AGGCTTTCTT CTCTGCTAAT 
GCTGCTGCAC AGGCCTCAAG GAAGTCCTCT CCAAGGGTGA CCAATGAGGC TGTTCAAAAG 
GCTGCTGCTG CTTTGAAGGG TTCTGACCAC CGCCGAGCTA CAAATGTTAG TGCCAGACTT 
GATGCCCAAC AGAAAAAGCT TAATCTTCCA ATCCTCCCAA CCACCACAAT TGGGTCCTTC 
CCACAGACCA TAGAACTCAG GAGAGTCCGC CGTGAATACA AGGCCAAAAA GGTCTCTGAG 
GACGATTACG TTAAAGCCAT CAAGGAGGAG ATCAGCAAAG TTGTCAAACT CCAAGAGGAG 
CTTGATATTG ATGTTCTAGT CCATGGAGAG CCTGAGAGAA ACGATATGGT TGAGTACTTT 
GGAGAGCAGT TGTCAGGTTT TGCCTTCACT GTCAATGGAT GGGTGCAATC TTATGGATCT 
CGATGTGTGA AGCCGCCAAT CATCTATGGT GATGTTAGCC GTCCCAACCC AATGACTGTC 
TTCTGGTCCT CGACAGCTCA GAGCATGACT GCCCGCCCAA TGAAGGGAAT GCTTACAGGC 
CCTGTCACCA TTCTCAACTG GTCCTTTGTC AGAAATGACC AGCCAAGATT TGAGACCTGT 
TATCAGATTG CTTTGGCCAT TAAGGATGAA GTGGAGGATC TTGAGAAGGC TGGTATCAAC 
GTTATCCAAA TCGATGAGGC TGCATTAAGA GAGGGTTTGC CTCTTAGGAA GTCCGAGCAA 
GCTTTCTACT TAGATTGGGC AGTGCACTCC TTCAGAATCA CCAATGTTGG AGTCCAGGAC 
ACCACCCAGA TCCACACTCA CATGTGCTAC TCCAACTTCA ATGACATTAT TCACTCAATT 
ATCGACATGG ATGCTGATGT GATCACCATT GAGAACTCCC GTTCTGATGA GAAGCTCCTT 
TCAGTGTTCC GTGAGGGAGT GAAGTATGGA GCTGGCATTG GCCCTGGTGT ATACGATATC 
CACTCTCCAA GAATACCATC AACTGAGGAA ATTGCTGACA GAATTAACAA GATGCTTGCA 
GTTCTTGAAA TGAACATCTT GTGGGTTAAC CCTGACTGCG GTCTCAAGAC TCGCAAGTAT 
TCAGAGGTCA AGCCAGCTCT CAGCAACATG GTCGCCGCAG CCAAGCTCCT CCGCACTCAG 
CTTGCTAGTG CTAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8282375 ( XM_025158541.1 ) cDNA ORF clone, Ricinus communis(castor bean) -> XP_025014309.1 Ricinus communis 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (LOC8282375), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORb21530
Clone ID Related Accession (Same CDS sequence)	XM_002525663.3 , XM_025158541.1 , XM_002525663.2
Accession Version	XM_025158541.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526918400000
Organism	Ricinus communis(castor bean)
Product	5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002994473.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ricinus communis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCTC ACATTGTTGG ATATCCTCGT ATGGGCCCTA AGAGAGAGCT CAAGTTTGCA 
TTGGAATCTT TTTGGGATGG AAAGAGCAGT GCTCAGGACT TGGAAAAGGT GGCATCTGAT 
CTCAGGGAAT CCATCTGGAA GCAGATGGCT GCTGCTGGGA TCAAACATAT CCCCAGCAAT 
ACTTTCTCTT ACTACGATCA GGTGCTTGAT ACCACAGCAA TGCTTGGAGC TGTTCCACCT 
AGATATGGCT GGAATGGTGG TGAGATTGGA TTCGATACAT ACTTCTCCAT GGCTAGAGGA 
AACGCCTCTG TGCCCGCTAT GGAAATGACC AAGTGGTTTG ACACCAATTA CCATTTCATT 
GTCCCTGAAT TGGGACCTGA TGTCAACTTC TCTTACGCTT CTCACAAAGC AGTGACCGAA 
TACAAGGAGG CTAAGGCGCA TGGAGTAGAC ACCGTTCCTG TTCTTGTTGG CCCTGTCTCT 
TATTTGTTAC TTTCTAAACC TGCCAAGGGT GTTGAGAAAA CCTTTTCTCT TCTCTCCCTT 
CTTGGAAAGA TCCTTCCAGT CTACAAGGAG GTTATTTCTG AGCTTAAAGC AGCTGGTGCA 
TCCTGGATTC AGTTTGATGA GCCTACACTT GTTTTGGATC TTGATTCTCA CAAACTGCAA 
GCATTTACTG ATGCCTACTC TGAGTTGGAG TCGACCCTAT CTGGCTTGAA TGTTCTCATT 
GAGACATACT TTGCTGACAT CCCAGCTGAG GCATTCAAGA CACTCACATC TCTTAAGGGC 
GTCACTGCAT ATGGTTTTGA TTTGGTTCGT GGAACTAAGA CCCTTGACTT GATCAAGAGT 
GAATTCCCCA AGGGCAAATA CCTATTTGCT GGAGTAGTTG ATGGAAGGAA TATTTGGGCC 
AATGATCTTG CTGCTTCTCT TACCATCTTG CATGAACTTG AAGGCATTGT GGGCAAGGAC 
AAACTCGTAG TCTCCACCTC TTCTTCACTT CTTCACACTG CTGTTGATCT CGTGAACGAG 
ACTAAGTTGG ACAAGGAACT AAAATCATGG CTTGCATTTG CTGCCCAGAA AATTGTCGAA 
GTTAATGCAT TGGCCAAGGC TTTGGCTGGT GAAAAAGATG AGGCTTTCTT CTCTGCTAAT 
GCTGCTGCAC AGGCCTCAAG GAAGTCCTCT CCAAGGGTGA CCAATGAGGC TGTTCAAAAG 
GCTGCTGCTG CTTTGAAGGG TTCTGACCAC CGCCGAGCTA CAAATGTTAG TGCCAGACTT 
GATGCCCAAC AGAAAAAGCT TAATCTTCCA ATCCTCCCAA CCACCACAAT TGGGTCCTTC 
CCACAGACCA TAGAACTCAG GAGAGTCCGC CGTGAATACA AGGCCAAAAA GGTCTCTGAG 
GACGATTACG TTAAAGCCAT CAAGGAGGAG ATCAGCAAAG TTGTCAAACT CCAAGAGGAG 
CTTGATATTG ATGTTCTAGT CCATGGAGAG CCTGAGAGAA ACGATATGGT TGAGTACTTT 
GGAGAGCAGT TGTCAGGTTT TGCCTTCACT GTCAATGGAT GGGTGCAATC TTATGGATCT 
CGATGTGTGA AGCCGCCAAT CATCTATGGT GATGTTAGCC GTCCCAACCC AATGACTGTC 
TTCTGGTCCT CGACAGCTCA GAGCATGACT GCCCGCCCAA TGAAGGGAAT GCTTACAGGC 
CCTGTCACCA TTCTCAACTG GTCCTTTGTC AGAAATGACC AGCCAAGATT TGAGACCTGT 
TATCAGATTG CTTTGGCCAT TAAGGATGAA GTGGAGGATC TTGAGAAGGC TGGTATCAAC 
GTTATCCAAA TCGATGAGGC TGCATTAAGA GAGGGTTTGC CTCTTAGGAA GTCCGAGCAA 
GCTTTCTACT TAGATTGGGC AGTGCACTCC TTCAGAATCA CCAATGTTGG AGTCCAGGAC 
ACCACCCAGA TCCACACTCA CATGTGCTAC TCCAACTTCA ATGACATTAT TCACTCAATT 
ATCGACATGG ATGCTGATGT GATCACCATT GAGAACTCCC GTTCTGATGA GAAGCTCCTT 
TCAGTGTTCC GTGAGGGAGT GAAGTATGGA GCTGGCATTG GCCCTGGTGT ATACGATATC 
CACTCTCCAA GAATACCATC AACTGAGGAA ATTGCTGACA GAATTAACAA GATGCTTGCA 
GTTCTTGAAA TGAACATCTT GTGGGTTAAC CCTGACTGCG GTCTCAAGAC TCGCAAGTAT 
TCAGAGGTCA AGCCAGCTCT CAGCAACATG GTCGCCGCAG CCAAGCTCCT CCGCACTCAG 
CTTGCTAGTG CTAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025014309.1
CDS	8..2305
Translation	MASHIVGYPRMGPKRELKFALESFWDGKSSAQDLEKVASDLRESIWKQMAAAGIKHIPSNTFSYYDQVLDTTAMLGAVPPRYGWNGGEIGFDTYFSMARGNASVPAMEMTKWFDTNYHFIVPELGPDVNFSYASHKAVTEYKEAKAHGVDTVPVLVGPVSYLLLSKPAKGVEKTFSLLSLLGKILPVYKEVISELKAAGASWIQFDEPTLVLDLDSHKLQAFTDAYSELESTLSGLNVLIETYFADIPAEAFKTLTSLKGVTAYGFDLVRGTKTLDLIKSEFPKGKYLFAGVVDGRNIWANDLAASLTILHELEGIVGKDKLVVSTSSSLLHTAVDLVNETKLDKELKSWLAFAAQKIVEVNALAKALAGEKDEAFFSANAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRATNVSARLDAQQKKLNLPILPTTTIGSFPQTIELRRVRREYKAKKVSEDDYVKAIKEEISKVVKLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRPNPMTVFWSSTAQSMTARPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEQAFYLDWAVHSFRITNVGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLEMNILWVNPDCGLKTRKYSEVKPALSNMVAAAKLLRTQLASAK

Target ORF information:

RefSeq Version	XM_025158541.1
Organism	Ricinus communis(castor bean)
Definition	Ricinus communis 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (LOC8282375), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025158541.1

ORF Insert Sequence:

ATGGCTTCTC ACATTGTTGG ATATCCTCGT ATGGGCCCTA AGAGAGAGCT CAAGTTTGCA 
TTGGAATCTT TTTGGGATGG AAAGAGCAGT GCTCAGGACT TGGAAAAGGT GGCATCTGAT 
CTCAGGGAAT CCATCTGGAA GCAGATGGCT GCTGCTGGGA TCAAACATAT CCCCAGCAAT 
ACTTTCTCTT ACTACGATCA GGTGCTTGAT ACCACAGCAA TGCTTGGAGC TGTTCCACCT 
AGATATGGCT GGAATGGTGG TGAGATTGGA TTCGATACAT ACTTCTCCAT GGCTAGAGGA 
AACGCCTCTG TGCCCGCTAT GGAAATGACC AAGTGGTTTG ACACCAATTA CCATTTCATT 
GTCCCTGAAT TGGGACCTGA TGTCAACTTC TCTTACGCTT CTCACAAAGC AGTGACCGAA 
TACAAGGAGG CTAAGGCGCA TGGAGTAGAC ACCGTTCCTG TTCTTGTTGG CCCTGTCTCT 
TATTTGTTAC TTTCTAAACC TGCCAAGGGT GTTGAGAAAA CCTTTTCTCT TCTCTCCCTT 
CTTGGAAAGA TCCTTCCAGT CTACAAGGAG GTTATTTCTG AGCTTAAAGC AGCTGGTGCA 
TCCTGGATTC AGTTTGATGA GCCTACACTT GTTTTGGATC TTGATTCTCA CAAACTGCAA 
GCATTTACTG ATGCCTACTC TGAGTTGGAG TCGACCCTAT CTGGCTTGAA TGTTCTCATT 
GAGACATACT TTGCTGACAT CCCAGCTGAG GCATTCAAGA CACTCACATC TCTTAAGGGC 
GTCACTGCAT ATGGTTTTGA TTTGGTTCGT GGAACTAAGA CCCTTGACTT GATCAAGAGT 
GAATTCCCCA AGGGCAAATA CCTATTTGCT GGAGTAGTTG ATGGAAGGAA TATTTGGGCC 
AATGATCTTG CTGCTTCTCT TACCATCTTG CATGAACTTG AAGGCATTGT GGGCAAGGAC 
AAACTCGTAG TCTCCACCTC TTCTTCACTT CTTCACACTG CTGTTGATCT CGTGAACGAG 
ACTAAGTTGG ACAAGGAACT AAAATCATGG CTTGCATTTG CTGCCCAGAA AATTGTCGAA 
GTTAATGCAT TGGCCAAGGC TTTGGCTGGT GAAAAAGATG AGGCTTTCTT CTCTGCTAAT 
GCTGCTGCAC AGGCCTCAAG GAAGTCCTCT CCAAGGGTGA CCAATGAGGC TGTTCAAAAG 
GCTGCTGCTG CTTTGAAGGG TTCTGACCAC CGCCGAGCTA CAAATGTTAG TGCCAGACTT 
GATGCCCAAC AGAAAAAGCT TAATCTTCCA ATCCTCCCAA CCACCACAAT TGGGTCCTTC 
CCACAGACCA TAGAACTCAG GAGAGTCCGC CGTGAATACA AGGCCAAAAA GGTCTCTGAG 
GACGATTACG TTAAAGCCAT CAAGGAGGAG ATCAGCAAAG TTGTCAAACT CCAAGAGGAG 
CTTGATATTG ATGTTCTAGT CCATGGAGAG CCTGAGAGAA ACGATATGGT TGAGTACTTT 
GGAGAGCAGT TGTCAGGTTT TGCCTTCACT GTCAATGGAT GGGTGCAATC TTATGGATCT 
CGATGTGTGA AGCCGCCAAT CATCTATGGT GATGTTAGCC GTCCCAACCC AATGACTGTC 
TTCTGGTCCT CGACAGCTCA GAGCATGACT GCCCGCCCAA TGAAGGGAAT GCTTACAGGC 
CCTGTCACCA TTCTCAACTG GTCCTTTGTC AGAAATGACC AGCCAAGATT TGAGACCTGT 
TATCAGATTG CTTTGGCCAT TAAGGATGAA GTGGAGGATC TTGAGAAGGC TGGTATCAAC 
GTTATCCAAA TCGATGAGGC TGCATTAAGA GAGGGTTTGC CTCTTAGGAA GTCCGAGCAA 
GCTTTCTACT TAGATTGGGC AGTGCACTCC TTCAGAATCA CCAATGTTGG AGTCCAGGAC 
ACCACCCAGA TCCACACTCA CATGTGCTAC TCCAACTTCA ATGACATTAT TCACTCAATT 
ATCGACATGG ATGCTGATGT GATCACCATT GAGAACTCCC GTTCTGATGA GAAGCTCCTT 
TCAGTGTTCC GTGAGGGAGT GAAGTATGGA GCTGGCATTG GCCCTGGTGT ATACGATATC 
CACTCTCCAA GAATACCATC AACTGAGGAA ATTGCTGACA GAATTAACAA GATGCTTGCA 
GTTCTTGAAA TGAACATCTT GTGGGTTAAC CCTGACTGCG GTCTCAAGAC TCGCAAGTAT 
TCAGAGGTCA AGCCAGCTCT CAGCAACATG GTCGCCGCAG CCAAGCTCCT CCGCACTCAG 
CTTGCTAGTG CTAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8282375 ( XM_002525663.2 ) cDNA ORF clone, Ricinus communis(castor bean) -> XP_002525709.1 Ricinus communis 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (LOC8282375), mRNA.

Price & Availability↑

CloneID	ORb21530
Clone ID Related Accession (Same CDS sequence)	XM_002525663.3 , XM_025158541.1 , XM_002525663.2
Accession Version	XM_002525663.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1456243200000
Organism	Ricinus communis(castor bean)
Product	5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002994473.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 25, 2016 this sequence version replaced XM_002525663.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ricinus communis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCTC ACATTGTTGG ATATCCTCGT ATGGGCCCTA AGAGAGAGCT CAAGTTTGCA 
TTGGAATCTT TTTGGGATGG AAAGAGCAGT GCTCAGGACT TGGAAAAGGT GGCATCTGAT 
CTCAGGGAAT CCATCTGGAA GCAGATGGCT GCTGCTGGGA TCAAACATAT CCCCAGCAAT 
ACTTTCTCTT ACTACGATCA GGTGCTTGAT ACCACAGCAA TGCTTGGAGC TGTTCCACCT 
AGATATGGCT GGAATGGTGG TGAGATTGGA TTCGATACAT ACTTCTCCAT GGCTAGAGGA 
AACGCCTCTG TGCCCGCTAT GGAAATGACC AAGTGGTTTG ACACCAATTA CCATTTCATT 
GTCCCTGAAT TGGGACCTGA TGTCAACTTC TCTTACGCTT CTCACAAAGC AGTGACCGAA 
TACAAGGAGG CTAAGGCGCA TGGAGTAGAC ACCGTTCCTG TTCTTGTTGG CCCTGTCTCT 
TATTTGTTAC TTTCTAAACC TGCCAAGGGT GTTGAGAAAA CCTTTTCTCT TCTCTCCCTT 
CTTGGAAAGA TCCTTCCAGT CTACAAGGAG GTTATTTCTG AGCTTAAAGC AGCTGGTGCA 
TCCTGGATTC AGTTTGATGA GCCTACACTT GTTTTGGATC TTGATTCTCA CAAACTGCAA 
GCATTTACTG ATGCCTACTC TGAGTTGGAG TCGACCCTAT CTGGCTTGAA TGTTCTCATT 
GAGACATACT TTGCTGACAT CCCAGCTGAG GCATTCAAGA CACTCACATC TCTTAAGGGC 
GTCACTGCAT ATGGTTTTGA TTTGGTTCGT GGAACTAAGA CCCTTGACTT GATCAAGAGT 
GAATTCCCCA AGGGCAAATA CCTATTTGCT GGAGTAGTTG ATGGAAGGAA TATTTGGGCC 
AATGATCTTG CTGCTTCTCT TACCATCTTG CATGAACTTG AAGGCATTGT GGGCAAGGAC 
AAACTCGTAG TCTCCACCTC TTCTTCACTT CTTCACACTG CTGTTGATCT CGTGAACGAG 
ACTAAGTTGG ACAAGGAACT AAAATCATGG CTTGCATTTG CTGCCCAGAA AATTGTCGAA 
GTTAATGCAT TGGCCAAGGC TTTGGCTGGT GAAAAAGATG AGGCTTTCTT CTCTGCTAAT 
GCTGCTGCAC AGGCCTCAAG GAAGTCCTCT CCAAGGGTGA CCAATGAGGC TGTTCAAAAG 
GCTGCTGCTG CTTTGAAGGG TTCTGACCAC CGCCGAGCTA CAAATGTTAG TGCCAGACTT 
GATGCCCAAC AGAAAAAGCT TAATCTTCCA ATCCTCCCAA CCACCACAAT TGGGTCCTTC 
CCACAGACCA TAGAACTCAG GAGAGTCCGC CGTGAATACA AGGCCAAAAA GGTCTCTGAG 
GACGATTACG TTAAAGCCAT CAAGGAGGAG ATCAGCAAAG TTGTCAAACT CCAAGAGGAG 
CTTGATATTG ATGTTCTAGT CCATGGAGAG CCTGAGAGAA ACGATATGGT TGAGTACTTT 
GGAGAGCAGT TGTCAGGTTT TGCCTTCACT GTCAATGGAT GGGTGCAATC TTATGGATCT 
CGATGTGTGA AGCCGCCAAT CATCTATGGT GATGTTAGCC GTCCCAACCC AATGACTGTC 
TTCTGGTCCT CGACAGCTCA GAGCATGACT GCCCGCCCAA TGAAGGGAAT GCTTACAGGC 
CCTGTCACCA TTCTCAACTG GTCCTTTGTC AGAAATGACC AGCCAAGATT TGAGACCTGT 
TATCAGATTG CTTTGGCCAT TAAGGATGAA GTGGAGGATC TTGAGAAGGC TGGTATCAAC 
GTTATCCAAA TCGATGAGGC TGCATTAAGA GAGGGTTTGC CTCTTAGGAA GTCCGAGCAA 
GCTTTCTACT TAGATTGGGC AGTGCACTCC TTCAGAATCA CCAATGTTGG AGTCCAGGAC 
ACCACCCAGA TCCACACTCA CATGTGCTAC TCCAACTTCA ATGACATTAT TCACTCAATT 
ATCGACATGG ATGCTGATGT GATCACCATT GAGAACTCCC GTTCTGATGA GAAGCTCCTT 
TCAGTGTTCC GTGAGGGAGT GAAGTATGGA GCTGGCATTG GCCCTGGTGT ATACGATATC 
CACTCTCCAA GAATACCATC AACTGAGGAA ATTGCTGACA GAATTAACAA GATGCTTGCA 
GTTCTTGAAA TGAACATCTT GTGGGTTAAC CCTGACTGCG GTCTCAAGAC TCGCAAGTAT 
TCAGAGGTCA AGCCAGCTCT CAGCAACATG GTCGCCGCAG CCAAGCTCCT CCGCACTCAG 
CTTGCTAGTG CTAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002525709.1
CDS	127..2424
Translation	MASHIVGYPRMGPKRELKFALESFWDGKSSAQDLEKVASDLRESIWKQMAAAGIKHIPSNTFSYYDQVLDTTAMLGAVPPRYGWNGGEIGFDTYFSMARGNASVPAMEMTKWFDTNYHFIVPELGPDVNFSYASHKAVTEYKEAKAHGVDTVPVLVGPVSYLLLSKPAKGVEKTFSLLSLLGKILPVYKEVISELKAAGASWIQFDEPTLVLDLDSHKLQAFTDAYSELESTLSGLNVLIETYFADIPAEAFKTLTSLKGVTAYGFDLVRGTKTLDLIKSEFPKGKYLFAGVVDGRNIWANDLAASLTILHELEGIVGKDKLVVSTSSSLLHTAVDLVNETKLDKELKSWLAFAAQKIVEVNALAKALAGEKDEAFFSANAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRATNVSARLDAQQKKLNLPILPTTTIGSFPQTIELRRVRREYKAKKVSEDDYVKAIKEEISKVVKLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRPNPMTVFWSSTAQSMTARPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLRKSEQAFYLDWAVHSFRITNVGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLEMNILWVNPDCGLKTRKYSEVKPALSNMVAAAKLLRTQLASAK

Target ORF information:

RefSeq Version	XM_002525663.2
Organism	Ricinus communis(castor bean)
Definition	Ricinus communis 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (LOC8282375), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002525663.2

ORF Insert Sequence:

ATGGCTTCTC ACATTGTTGG ATATCCTCGT ATGGGCCCTA AGAGAGAGCT CAAGTTTGCA 
TTGGAATCTT TTTGGGATGG AAAGAGCAGT GCTCAGGACT TGGAAAAGGT GGCATCTGAT 
CTCAGGGAAT CCATCTGGAA GCAGATGGCT GCTGCTGGGA TCAAACATAT CCCCAGCAAT 
ACTTTCTCTT ACTACGATCA GGTGCTTGAT ACCACAGCAA TGCTTGGAGC TGTTCCACCT 
AGATATGGCT GGAATGGTGG TGAGATTGGA TTCGATACAT ACTTCTCCAT GGCTAGAGGA 
AACGCCTCTG TGCCCGCTAT GGAAATGACC AAGTGGTTTG ACACCAATTA CCATTTCATT 
GTCCCTGAAT TGGGACCTGA TGTCAACTTC TCTTACGCTT CTCACAAAGC AGTGACCGAA 
TACAAGGAGG CTAAGGCGCA TGGAGTAGAC ACCGTTCCTG TTCTTGTTGG CCCTGTCTCT 
TATTTGTTAC TTTCTAAACC TGCCAAGGGT GTTGAGAAAA CCTTTTCTCT TCTCTCCCTT 
CTTGGAAAGA TCCTTCCAGT CTACAAGGAG GTTATTTCTG AGCTTAAAGC AGCTGGTGCA 
TCCTGGATTC AGTTTGATGA GCCTACACTT GTTTTGGATC TTGATTCTCA CAAACTGCAA 
GCATTTACTG ATGCCTACTC TGAGTTGGAG TCGACCCTAT CTGGCTTGAA TGTTCTCATT 
GAGACATACT TTGCTGACAT CCCAGCTGAG GCATTCAAGA CACTCACATC TCTTAAGGGC 
GTCACTGCAT ATGGTTTTGA TTTGGTTCGT GGAACTAAGA CCCTTGACTT GATCAAGAGT 
GAATTCCCCA AGGGCAAATA CCTATTTGCT GGAGTAGTTG ATGGAAGGAA TATTTGGGCC 
AATGATCTTG CTGCTTCTCT TACCATCTTG CATGAACTTG AAGGCATTGT GGGCAAGGAC 
AAACTCGTAG TCTCCACCTC TTCTTCACTT CTTCACACTG CTGTTGATCT CGTGAACGAG 
ACTAAGTTGG ACAAGGAACT AAAATCATGG CTTGCATTTG CTGCCCAGAA AATTGTCGAA 
GTTAATGCAT TGGCCAAGGC TTTGGCTGGT GAAAAAGATG AGGCTTTCTT CTCTGCTAAT 
GCTGCTGCAC AGGCCTCAAG GAAGTCCTCT CCAAGGGTGA CCAATGAGGC TGTTCAAAAG 
GCTGCTGCTG CTTTGAAGGG TTCTGACCAC CGCCGAGCTA CAAATGTTAG TGCCAGACTT 
GATGCCCAAC AGAAAAAGCT TAATCTTCCA ATCCTCCCAA CCACCACAAT TGGGTCCTTC 
CCACAGACCA TAGAACTCAG GAGAGTCCGC CGTGAATACA AGGCCAAAAA GGTCTCTGAG 
GACGATTACG TTAAAGCCAT CAAGGAGGAG ATCAGCAAAG TTGTCAAACT CCAAGAGGAG 
CTTGATATTG ATGTTCTAGT CCATGGAGAG CCTGAGAGAA ACGATATGGT TGAGTACTTT 
GGAGAGCAGT TGTCAGGTTT TGCCTTCACT GTCAATGGAT GGGTGCAATC TTATGGATCT 
CGATGTGTGA AGCCGCCAAT CATCTATGGT GATGTTAGCC GTCCCAACCC AATGACTGTC 
TTCTGGTCCT CGACAGCTCA GAGCATGACT GCCCGCCCAA TGAAGGGAAT GCTTACAGGC 
CCTGTCACCA TTCTCAACTG GTCCTTTGTC AGAAATGACC AGCCAAGATT TGAGACCTGT 
TATCAGATTG CTTTGGCCAT TAAGGATGAA GTGGAGGATC TTGAGAAGGC TGGTATCAAC 
GTTATCCAAA TCGATGAGGC TGCATTAAGA GAGGGTTTGC CTCTTAGGAA GTCCGAGCAA 
GCTTTCTACT TAGATTGGGC AGTGCACTCC TTCAGAATCA CCAATGTTGG AGTCCAGGAC 
ACCACCCAGA TCCACACTCA CATGTGCTAC TCCAACTTCA ATGACATTAT TCACTCAATT 
ATCGACATGG ATGCTGATGT GATCACCATT GAGAACTCCC GTTCTGATGA GAAGCTCCTT 
TCAGTGTTCC GTGAGGGAGT GAAGTATGGA GCTGGCATTG GCCCTGGTGT ATACGATATC 
CACTCTCCAA GAATACCATC AACTGAGGAA ATTGCTGACA GAATTAACAA GATGCTTGCA 
GTTCTTGAAA TGAACATCTT GTGGGTTAAC CCTGACTGCG GTCTCAAGAC TCGCAAGTAT 
TCAGAGGTCA AGCCAGCTCT CAGCAACATG GTCGCCGCAG CCAAGCTCCT CCGCACTCAG 
CTTGCTAGTG CTAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC8282375 cDNA ORF clone, Ricinus communis(castor bean)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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