Timm8a1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Timm8a1
Entrez Gene ID	84383
Full Name	translocase of inner mitochondrial membrane 8 homolog A1 (yeast)
Synonyms	DDP,Ddp1,Timm8a
General protein information	Preferred Names translocase of inner mitochondrial membrane 8 homolog A1 (yeast) Names mitochondrial import inner membrane translocase subunit Tim8 A deafness dystonia protein 1 homolog translocase of inner mitochondrial membrane 8 homolog a
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: X Map Location: Xq32
Summary	mediates mitochondrial protein import; may play a role in cell signaling and endosomal trafficking; deletion in human homolog causes X-linked degenerative dystonia, Mohr-Tranebjaerg-Jensen deafness-dystonia-optic atrophysyndrome [RGD, Jun 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053370.3	NP_445822.1	mitochondrial import inner membrane translocase subunit Tim8 A

Pathways from BioSystems

Homologies

Homo sapiens (human)	TIMM8A	NP_004076.1
Rattus norvegicus (Norway rat)	Timm8a1	NP_445822.1
Bos taurus (cattle)	TIMM8A	NP_001029652.1
Pan troglodytes (chimpanzee)	TIMM8A	XP_001139457.1
Canis lupus familiaris (dog)	TIMM8A	NP_001239296.1
Mus musculus (house mouse)	Timm8a1	NP_038926.1
Danio rerio (zebrafish)	timm8a	NP_001003637.1
Macaca mulatta (Rhesus monkey)	TIMM8A	XP_001092722.1
Gallus gallus (chicken)	TIMM8A	XP_420185.1
Xenopus tropicalis (tropical clawed frog)	timm8a	XP_002940448.1

Gene Ontology
Bibliography

Related articles in PubMed

Assembly of β-barrel proteins in the mitochondrial outer membrane.
Höhr AI, Straub SP, Warscheid B, Becker T, Wiedemann N
Biochimica et biophysica acta1853(1)74-88(2015 Jan)

Phosphoproteome analysis of functional mitochondria isolated from resting human muscle reveals extensive phosphorylation of inner membrane protein complexes and enzymes.
Zhao X, León IR, Bak S, Mogensen M, Wrzesinski K, Højlund K, Jensen ON
Molecular & cellular proteomics : MCP10(1)M110.000299(2011 Jan)

Otopathology in Mohr-Tranebjaerg syndrome.
Bahmad F Jr, Merchant SN, Nadol JB Jr, Tranebjaerg L
The Laryngoscope117(7)1202-8(2007 Jul)

A novel intronic mutation in the DDP1 gene in a family with X-linked dystonia-deafness syndrome.
Ezquerra M, Campdelacreu J, Muñoz E, Tolosa E, Martí MJ
Archives of neurology62(2)306-8(2005 Feb)

Organization and function of the small Tim complexes acting along the import pathway of metabolite carriers into mammalian mitochondria.
Mühlenbein N, Hofmann S, Rothbauer U, Bauer MF
The Journal of biological chemistry279(14)13540-6(2004 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Timm8a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Timm8a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12330	NM_053370.3 Latest version!	Rattus norvegicus translocase of inner mitochondrial membrane 8A1 (Timm8a1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Timm8a1 ( NM_053370.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_445822.1 Rattus norvegicus translocase of inner mitochondrial membrane 8A1 (Timm8a1), mRNA.

Price & Availability↑

CloneID	ORa12330
Clone ID Related Accession (Same CDS sequence)	NM_053370.3
Accession Version	NM_053370.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569168000000
Organism	Rattus norvegicus(Norway rat)
Product	mitochondrial import inner membrane translocase subunit Tim8 A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC060552.1. On Nov 28, 2013 this sequence version replaced NM_053370.2. ##Evidence-Data-START## Transcript exon combination :: BC060552.1, EV773057.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGAGTCCT CCTCGTCCTC TTCGGGCTCG GCTTTGGCCG CCGTGGACCC TCAGCTGCAG 
CATTTCATCG AGGTGGAGAC ACAGAAGCAG CGCTTCCAGC AGCTGGTGCA CCAGATGACG 
GAACTTTGCT GGGAGAAGTG CATGGATAAG CCTGGGCCTA AGTTGGACAG TCGGGCTGAG 
GCCTGTTTTG TGAACTGCGT TGAACGCTTC ATTGATACAA GCCAGTTCAT CTTAAATCGA 
CTGGAGCAGA CCCAGAAGTC CAAACCTGTC TTCTCAGAAA GCCTTTCTGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_445822.1
CDS	27..320
Translation	MESSSSSSGSALAAVDPQLQHFIEVETQKQRFQQLVHQMTELCWEKCMDKPGPKLDSRAEACFVNCVERFIDTSQFILNRLEQTQKSKPVFSESLSD

Target ORF information:

RefSeq Version	NM_053370.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus translocase of inner mitochondrial membrane 8A1 (Timm8a1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053370.3

ORF Insert Sequence:

ATGGAGTCCT CCTCGTCCTC TTCGGGCTCG GCTTTGGCCG CCGTGGACCC TCAGCTGCAG 
CATTTCATCG AGGTGGAGAC ACAGAAGCAG CGCTTCCAGC AGCTGGTGCA CCAGATGACG 
GAACTTTGCT GGGAGAAGTG CATGGATAAG CCTGGGCCTA AGTTGGACAG TCGGGCTGAG 
GCCTGTTTTG TGAACTGCGT TGAACGCTTC ATTGATACAA GCCAGTTCAT CTTAAATCGA 
CTGGAGCAGA CCCAGAAGTC CAAACCTGTC TTCTCAGAAA GCCTTTCTGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Assembly of β-barrel proteins in the mitochondrial outer membrane.
Biochimica et biophysica acta1853(1)74-88(2015 Jan)
Höhr AI,Straub SP,Warscheid B,Becker T,Wiedemann N

Phosphoproteome analysis of functional mitochondria isolated from resting human muscle reveals extensive phosphorylation of inner membrane protein complexes and enzymes.
Molecular & cellular proteomics : MCP10(1)M110.000299(2011 Jan)
Zhao X,León IR,Bak S,Mogensen M,Wrzesinski K,Højlund K,Jensen ON

Otopathology in Mohr-Tranebjaerg syndrome.
The Laryngoscope117(7)1202-8(2007 Jul)
Bahmad F Jr,Merchant SN,Nadol JB Jr,Tranebjaerg L

A novel intronic mutation in the DDP1 gene in a family with X-linked dystonia-deafness syndrome.
Archives of neurology62(2)306-8(2005 Feb)
Ezquerra M,Campdelacreu J,Muñoz E,Tolosa E,Martí MJ

Organization and function of the small Tim complexes acting along the import pathway of metabolite carriers into mammalian mitochondria.
The Journal of biological chemistry279(14)13540-6(2004 Apr)
Mühlenbein N,Hofmann S,Rothbauer U,Bauer MF