Prep cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prep
Entrez Gene ID	83471
Full Name	prolyl endopeptidase
Synonyms	rPop
General protein information	Preferred Names prolyl endopeptidase Names prolyl endopeptidase PE post-proline cleaving enzyme
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 20 Map Location: 20q13
Summary	may play a role in neuropeptide catabolism; expressed in brain; most enzyme activity is localized to the cytosolic fraction [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031324.1	NP_112614.1	prolyl endopeptidase

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PREP	XP_518657.2
Rattus norvegicus (Norway rat)	Prep	NP_112614.1
Danio rerio (zebrafish)	prep	NP_001018589.1
Arabidopsis thaliana (thale cress)	AT1G76140	NP_177741.3
Xenopus tropicalis (tropical clawed frog)	prep	NP_989189.1
Drosophila melanogaster (fruit fly)	CG2528	NP_610129.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009172	XP_319935.4
Macaca mulatta (Rhesus monkey)	PREP	XP_001087580.1
Gallus gallus (chicken)	PREP	NP_001006410.1
Arabidopsis thaliana (thale cress)	AT1G20380	NP_173463.1
Oryza sativa (rice)	Os01g0108200	NP_001041787.1
Drosophila melanogaster (fruit fly)	CG5355	NP_609397.2
Homo sapiens (human)	PREP	NP_002717.3
Canis lupus familiaris (dog)	PREP	XP_005627728.1
Bos taurus (cattle)	PREP	NP_777197.1
Mus musculus (house mouse)	Prep	NP_035286.1

Gene Ontology
Bibliography

Related articles in PubMed

Post-Weaning Social Isolation Disturbs Gene Expression in Rat Brain Structures.
Zubkov EA, Zorkina YA, Orshanskaya EV, Khlebnikova NN, Krupina NA, Chekhonin VP
Bulletin of experimental biology and medicine166(3)364-368(2019 Jan)

Effect of prolyl endopeptidase inhibitor benzyloxycarbonyl-methionyl-2(S)-cyanopyrrolidine on activity of proline-specific peptidases in brain structures of rats with experimental MPTP-induced depressive syndrome.
Khlebnikova NN, Krupina NA, Bogdanova NG, Zolotov NN
Bulletin of experimental biology and medicine155(6)711-4(2013 Oct)

Inhibition of prolyl oligopeptidase by KYP-2047 fails to increase the extracellular neurotensin and substance P levels in rat striatum.
Jalkanen AJ, Savolainen K, Forsberg MM
Neuroscience letters502(2)107-11(2011 Sep)

Sequential expression, activity and nuclear localization of prolyl oligopeptidase protein in the developing rat brain.
Hannula MJ, Männistö PT, Myöhänen TT
Developmental neuroscience33(1)38-47(2011)

Prolyl endopeptidase mRNA expression in the central nervous system during rat development.
Agirregoitia N, Bizet P, Agirregoitia E, Boutelet I, Peralta L, Vaudry H, Jégou S
Journal of chemical neuroanatomy40(1)53-62(2010 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Prep gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prep cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa07818	NM_031324.1 Latest version!	Rattus norvegicus prolyl endopeptidase (Prep), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prep ( NM_031324.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_112614.1 Rattus norvegicus prolyl endopeptidase (Prep), mRNA.

Price & Availability↑

CloneID	ORa07818
Clone ID Related Accession (Same CDS sequence)	NM_031324.1
Accession Version	NM_031324.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2133bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577203200000
Organism	Rattus norvegicus(Norway rat)
Product	prolyl endopeptidase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB012759.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB012759.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGCTGTCCT TCCAGTACCC TGACGTGTAC CGCGACGAGA CCTCAGTACA GGATTATCAT 
GGACATAAAA TTTGCGACCC TTATGCTTGG CTTGAAGACC CAGACAGTGA ACAGACAAAG 
GCTTTCGTGG AGGCACAGAA CAAGATCACA GTGCCGTTTC TTGAGCAGTG TCCAATCAGA 
GGTCTATACA AGGAGAGGAT GACTGAGCTG TATGACTACC CCAAGTACAG CTGCCACTTC 
AAGAAAGGAA AGCGGTATTT TTATTTTTAC AATACAGGTT TGCAGAACCA GCGAGTATTA 
TATGTACAGG ACTCCTTAGA GGGGGAAGCC AGAGTGTTCC TGGATCCCAA CACTCTGTCG 
GATGACGGCA CGGTGGCACT CCGAGGTTAT GCCTTCAGCG AAGATGGTGA ATACTTCGCC 
TATGGTCTGA GCGCCAGCGG CTCAGACTGG GTGACCATCA AGTTCATGAA GGTCGACGGT 
GCCAAAGAGC TTCCCGACGT GCTGGAGAGA GTCAAGTTCA CCTGTATGGC CTGGACGCAC 
GATGGCAAAG GAATGTTCTA CAACTCATAC CCACAGCAGG ACGGCAAGAG CGACGGCACA 
GAGACGTCCA CCAATCTCCA CCAGAAGCTC TGTTATCACG TCTTGGGGAC GGATCAGTCA 
GAAGACGTTC TGTGTGCCGA GTTCCCCGAT GAGCCTAAAT GGATGGGAGG AGCCGAGTTA 
TCCGATGACG GTCGCTATGT CTTGTTATCG ATCTGGGAGG GATGTGATCC AGTGAATCGG 
CTGTGGTACT GTGACCTCCA GCAGGGATCC AATGGTATCA ACGGGATCCT GAAGTGGGTA 
AAACTGATAG ACAACTTTGA AGGGGAGTAC GATTACATAA CCAACGAGGG TACGGTGTTC 
ACCTTCAAGA CCAACCGCAA CTCCCCCAAT TACCGGCTGA TCAACATCGA CTTCACGGAC 
CCCGACGAGT CCAAGTGGAA AGTTCTCGTT CCGGAGCACG AGAAAGATGT CTTAGAGTGG 
GTGGCTTGCG TCAGGTCCAA CTTCCTGGTC TTGTGCTACC TCCGCAACGT GAAGAACATT 
CTGCAGCTTC ACGACCTGAC AACTGGTGCT CTCCTCAAGA CCTTCCCGCT GGACGTGGGC 
AGCGTGGTGG GGTACAGTGG GCGCAAGAAG GACTCTGAGA TCTTCTACCA GTTCACATCC 
TTCTTATCTC CAGGTGTCAT TTACCACTGT GATCTTACCA GAGAGGAACT GGAACCCCGG 
GTTTTCCGAG AGGTGACGGT GAAGGGAATC GATGCGTCTG ATTACCAGAC TATCCAGGTT 
TTCTACCCTA GCAAGGATGG CACAAAGATT CCCATGTTTA TTGTGCATAA AAAGGGCATA 
AAGTTGGATG GCTCGCATCC CGCTTTCCTG TATGGCTATG GTGGCTTCAA CATCTCCATC 
ACACCCAACT ACAGTGTGTC CAGGCTCATT TTTGTGAGAC ATATGGGCGG CGTCCTCGCG 
GTAGCTAACA TCAGAGGCGG TGGTGAATAC GGAGAAACAT GGCATAAAGG TGGGATCTTG 
GCCAACAAGC AGAACTGCTT TGATGACTTC CAGTGTGCTG CTGAGTACCT CATCAAGGAA 
GGCTACACAA CTTCCAAGAG GCTCACGATC AATGGAGGCT CCAATGGCGG CCTCTTAGTG 
GCTGCTTGCG CAAATCAGCG CCCGGACCTC TTCGGCTGTG TGATCGCCCA GGTTGGAGTG 
ATGGACATGT TGAAGTTCCA CAAATTTACT ATTGGCCATG CCTGGACCAC TGATTACGGG 
TGCTCCGACA GCAAACAACA CTTCGAATGG CTTCTCAAGT ACTCGCCGCT GCACAACGTG 
AAGCTTCCGG AGGCGGATGA CATCCAGTAC CCGTCCATGC TCCTCCTCAC GGCTGACCAC 
GACGACCGCG TGGTCCCGCT GCACTCGCTC AAGTTCATCG CCACGCTTCA GTACATCGTG 
GGCCGCAGCC GGAAGCAGAG CAACCCCCTG CTCATCCACG TGGACACCAA GGCCGGCCAC 
GGGCCGGGAA AACCCACCGC CAAGGTGATA GAAGAAGTCT CGGATATGTT TGCGTTCATA 
GCGCGGTGCC TGAACATCGA GTGGATCCAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112614.1
CDS	132..2264
Translation	MLSFQYPDVYRDETSVQDYHGHKICDPYAWLEDPDSEQTKAFVEAQNKITVPFLEQCPIRGLYKERMTELYDYPKYSCHFKKGKRYFYFYNTGLQNQRVLYVQDSLEGEARVFLDPNTLSDDGTVALRGYAFSEDGEYFAYGLSASGSDWVTIKFMKVDGAKELPDVLERVKFTCMAWTHDGKGMFYNSYPQQDGKSDGTETSTNLHQKLCYHVLGTDQSEDVLCAEFPDEPKWMGGAELSDDGRYVLLSIWEGCDPVNRLWYCDLQQGSNGINGILKWVKLIDNFEGEYDYITNEGTVFTFKTNRNSPNYRLINIDFTDPDESKWKVLVPEHEKDVLEWVACVRSNFLVLCYLRNVKNILQLHDLTTGALLKTFPLDVGSVVGYSGRKKDSEIFYQFTSFLSPGVIYHCDLTREELEPRVFREVTVKGIDASDYQTIQVFYPSKDGTKIPMFIVHKKGIKLDGSHPAFLYGYGGFNISITPNYSVSRLIFVRHMGGVLAVANIRGGGEYGETWHKGGILANKQNCFDDFQCAAEYLIKEGYTTSKRLTINGGSNGGLLVAACANQRPDLFGCVIAQVGVMDMLKFHKFTIGHAWTTDYGCSDSKQHFEWLLKYSPLHNVKLPEADDIQYPSMLLLTADHDDRVVPLHSLKFIATLQYIVGRSRKQSNPLLIHVDTKAGHGPGKPTAKVIEEVSDMFAFIARCLNIEWIQ

Target ORF information:

RefSeq Version	NM_031324.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus prolyl endopeptidase (Prep), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031324.1

ORF Insert Sequence:

ATGCTGTCCT TCCAGTACCC TGACGTGTAC CGCGACGAGA CCTCAGTACA GGATTATCAT 
GGACATAAAA TTTGCGACCC TTATGCTTGG CTTGAAGACC CAGACAGTGA ACAGACAAAG 
GCTTTCGTGG AGGCACAGAA CAAGATCACA GTGCCGTTTC TTGAGCAGTG TCCAATCAGA 
GGTCTATACA AGGAGAGGAT GACTGAGCTG TATGACTACC CCAAGTACAG CTGCCACTTC 
AAGAAAGGAA AGCGGTATTT TTATTTTTAC AATACAGGTT TGCAGAACCA GCGAGTATTA 
TATGTACAGG ACTCCTTAGA GGGGGAAGCC AGAGTGTTCC TGGATCCCAA CACTCTGTCG 
GATGACGGCA CGGTGGCACT CCGAGGTTAT GCCTTCAGCG AAGATGGTGA ATACTTCGCC 
TATGGTCTGA GCGCCAGCGG CTCAGACTGG GTGACCATCA AGTTCATGAA GGTCGACGGT 
GCCAAAGAGC TTCCCGACGT GCTGGAGAGA GTCAAGTTCA CCTGTATGGC CTGGACGCAC 
GATGGCAAAG GAATGTTCTA CAACTCATAC CCACAGCAGG ACGGCAAGAG CGACGGCACA 
GAGACGTCCA CCAATCTCCA CCAGAAGCTC TGTTATCACG TCTTGGGGAC GGATCAGTCA 
GAAGACGTTC TGTGTGCCGA GTTCCCCGAT GAGCCTAAAT GGATGGGAGG AGCCGAGTTA 
TCCGATGACG GTCGCTATGT CTTGTTATCG ATCTGGGAGG GATGTGATCC AGTGAATCGG 
CTGTGGTACT GTGACCTCCA GCAGGGATCC AATGGTATCA ACGGGATCCT GAAGTGGGTA 
AAACTGATAG ACAACTTTGA AGGGGAGTAC GATTACATAA CCAACGAGGG TACGGTGTTC 
ACCTTCAAGA CCAACCGCAA CTCCCCCAAT TACCGGCTGA TCAACATCGA CTTCACGGAC 
CCCGACGAGT CCAAGTGGAA AGTTCTCGTT CCGGAGCACG AGAAAGATGT CTTAGAGTGG 
GTGGCTTGCG TCAGGTCCAA CTTCCTGGTC TTGTGCTACC TCCGCAACGT GAAGAACATT 
CTGCAGCTTC ACGACCTGAC AACTGGTGCT CTCCTCAAGA CCTTCCCGCT GGACGTGGGC 
AGCGTGGTGG GGTACAGTGG GCGCAAGAAG GACTCTGAGA TCTTCTACCA GTTCACATCC 
TTCTTATCTC CAGGTGTCAT TTACCACTGT GATCTTACCA GAGAGGAACT GGAACCCCGG 
GTTTTCCGAG AGGTGACGGT GAAGGGAATC GATGCGTCTG ATTACCAGAC TATCCAGGTT 
TTCTACCCTA GCAAGGATGG CACAAAGATT CCCATGTTTA TTGTGCATAA AAAGGGCATA 
AAGTTGGATG GCTCGCATCC CGCTTTCCTG TATGGCTATG GTGGCTTCAA CATCTCCATC 
ACACCCAACT ACAGTGTGTC CAGGCTCATT TTTGTGAGAC ATATGGGCGG CGTCCTCGCG 
GTAGCTAACA TCAGAGGCGG TGGTGAATAC GGAGAAACAT GGCATAAAGG TGGGATCTTG 
GCCAACAAGC AGAACTGCTT TGATGACTTC CAGTGTGCTG CTGAGTACCT CATCAAGGAA 
GGCTACACAA CTTCCAAGAG GCTCACGATC AATGGAGGCT CCAATGGCGG CCTCTTAGTG 
GCTGCTTGCG CAAATCAGCG CCCGGACCTC TTCGGCTGTG TGATCGCCCA GGTTGGAGTG 
ATGGACATGT TGAAGTTCCA CAAATTTACT ATTGGCCATG CCTGGACCAC TGATTACGGG 
TGCTCCGACA GCAAACAACA CTTCGAATGG CTTCTCAAGT ACTCGCCGCT GCACAACGTG 
AAGCTTCCGG AGGCGGATGA CATCCAGTAC CCGTCCATGC TCCTCCTCAC GGCTGACCAC 
GACGACCGCG TGGTCCCGCT GCACTCGCTC AAGTTCATCG CCACGCTTCA GTACATCGTG 
GGCCGCAGCC GGAAGCAGAG CAACCCCCTG CTCATCCACG TGGACACCAA GGCCGGCCAC 
GGGCCGGGAA AACCCACCGC CAAGGTGATA GAAGAAGTCT CGGATATGTT TGCGTTCATA 
GCGCGGTGCC TGAACATCGA GTGGATCCAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Post-Weaning Social Isolation Disturbs Gene Expression in Rat Brain Structures.
Bulletin of experimental biology and medicine166(3)364-368(2019 Jan)
Zubkov EA,Zorkina YA,Orshanskaya EV,Khlebnikova NN,Krupina NA,Chekhonin VP

Effect of prolyl endopeptidase inhibitor benzyloxycarbonyl-methionyl-2(S)-cyanopyrrolidine on activity of proline-specific peptidases in brain structures of rats with experimental MPTP-induced depressive syndrome.
Bulletin of experimental biology and medicine155(6)711-4(2013 Oct)
Khlebnikova NN,Krupina NA,Bogdanova NG,Zolotov NN

Inhibition of prolyl oligopeptidase by KYP-2047 fails to increase the extracellular neurotensin and substance P levels in rat striatum.
Neuroscience letters502(2)107-11(2011 Sep)
Jalkanen AJ,Savolainen K,Forsberg MM

Sequential expression, activity and nuclear localization of prolyl oligopeptidase protein in the developing rat brain.
Developmental neuroscience33(1)38-47(2011)
Hannula MJ,Männistö PT,Myöhänen TT

Prolyl endopeptidase mRNA expression in the central nervous system during rat development.
Journal of chemical neuroanatomy40(1)53-62(2010 Sep)
Agirregoitia N,Bizet P,Agirregoitia E,Boutelet I,Peralta L,Vaudry H,Jégou S