Tctn2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tctn2
Entrez Gene ID	689779
Full Name	tectonic family member 2
Synonyms	Tect2
General protein information	Preferred Names tectonic family member 2 Names tectonic-2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 12 Map Location: 12q15

mRNA and Protein(s)

mRNA	Protein	Name
XM_008769263.2	XP_008767485.1	tectonic-2 isoform X1
NM_001080782.1	NP_001074251.1	tectonic-2 precursor

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	tctn2	XP_692290.5
Macaca mulatta (Rhesus monkey)	TCTN2	XP_001117556.1
Mus musculus (house mouse)	Tctn2	NP_080762.1
Homo sapiens (human)	TCTN2	NP_079085.2
Canis lupus familiaris (dog)	TCTN2	XP_534643.2
Bos taurus (cattle)	TCTN2	XP_003587192.1
Gallus gallus (chicken)	TCTN2	XP_003642239.2
Pan troglodytes (chimpanzee)	TCTN2	XP_003314062.1
Rattus norvegicus (Norway rat)	Tctn2	NP_001074251.1
Xenopus tropicalis (tropical clawed frog)	tctn2	NP_001039131.1

Gene Ontology
Bibliography

Related articles in PubMed

MKS5 and CEP290 Dependent Assembly Pathway of the Ciliary Transition Zone.
Li C, Jensen VL, Park K, Kennedy J, Garcia-Gonzalo FR, Romani M, De Mori R, Bruel AL, Gaillard D, Doray B, Lopez E, Rivière JB, Faivre L, Thauvin-Robinet C, Reiter JF, Blacque OE, Valente EM, Leroux MR
PLoS biology14(3)e1002416(2016 Mar)

A ciliopathy complex at the transition zone protects the cilia as a privileged membrane domain.
Chih B, Liu P, Chinn Y, Chalouni C, Komuves LG, Hass PE, Sandoval W, Peterson AS
Nature cell biology14(1)61-72(2011 Dec)

A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition.
Garcia-Gonzalo FR, Corbit KC, Sirerol-Piquer MS, Ramaswami G, Otto EA, Noriega TR, Seol AD, Robinson JF, Bennett CL, Josifova DJ, García-Verdugo JM, Katsanis N, Hildebrandt F, Reiter JF
Nature genetics43(8)776-84(2011 Jul)

Mapping the NPHP-JBTS-MKS protein network reveals ciliopathy disease genes and pathways.
Sang L, Miller JJ, Corbit KC, Giles RH, Brauer MJ, Otto EA, Baye LM, Wen X, Scales SJ, Kwong M, Huntzicker EG, Sfakianos MK, Sandoval W, Bazan JF, Kulkarni P, Garcia-Gonzalo FR, Seol AD, O'Toole JF, Held S, Reutter HM, Lane WS, Rafiq MA, Noor A, Ansar M, Devi AR, Sheffield VC, Slusarski DC, Vincent JB, Doherty DA, Hildebrandt F, Reiter JF, Jackson PK
Cell145(4)513-28(2011 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Tctn2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tctn2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa09668	NM_001080782.1 Latest version!	Rattus norvegicus tectonic family member 2 (Tctn2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ORa50392	XM_008769263.2 Latest version!	Rattus norvegicus tectonic family member 2 (Tctn2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tctn2 ( NM_001080782.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001074251.1 Rattus norvegicus tectonic family member 2 (Tctn2), mRNA.

Price & Availability↑

CloneID	ORa09668
Clone ID Related Accession (Same CDS sequence)	NM_001080782.1
Accession Version	NM_001080782.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550246400000
Organism	Rattus norvegicus(Norway rat)
Product	tectonic-2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC107658.1. On Jan 27, 2007 this sequence version replaced XM_001071967.1. ##Evidence-Data-START## Transcript exon combination :: BC107658.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGTTCTC TGTCGCCGCT CAGCTTCCTG TGGGGACTCC TCCTGCTGCA GGGCGTCCTG 
AGGCCGCTGC GCGGCGACCC GGTTTTCATC CCACCTTTCA TCCGGATGTC CAGTCCTGAG 
GTCCGCGCGT CCCTGGTCGG AGGCAGCGAG GATGTCACCG TGTCACTGAC TCCACTGCAA 
ATCAAGGAAG GAGTGTTGCC AGTTCCTACT TGTGGTGGCC TCAGGAATGA GACCGGGGAT 
TGGAATCTGA CTGTGAGCCC CCAAGCGAAC ATGCTGGAAG TGACCGTAAG GTGGAAGAGG 
GGTCTGGACT GGTGCTCTCC AGATGAGACA GCCTCGTTCT CAGAGGCCCC ATGCATCGTG 
CAGACTCTGC TGGTCTCAGC ATCCCACAAT GCATCCTGCT TAGCACATCT GCTCATCCAG 
GTGGAGATTT ATCCCAACAC GTCTGTGACC CACAATGCAT CAGAGAACAT GACTGTCATT 
CCTAACCAAG TCTATCAGCC CCTGGGTCCC TGCCCGTGTG ACTTGACAGC CAAGGCCTGT 
GACATTCGCT GCTGCTGTGA CCAAGACTGT CAGCCGGAGC TAAGAGAGCT GTTCGAACGG 
TTCTGCTTCT CCGGCGTGTT TGGCGGGTAC GTCAGCCCTC CGTCCCACCA TCGCTGTGCA 
GCCCGCACTA CTCACCAGAC TCCTGACTGG TTTCCCTTCC TGTGTGTGCA GTCACCACCC 
TCCACCTCTC CGTTCCTCGG TCACTTCTAC CACGGCGCCA TTTCCCCCAG ACATTCCCCC 
GGCTTTGAAA CCCATTCGCA CTTGGACCTA AGAGACTTCT TTGCTGACGC CAGCTACAAA 
CAAGGGGATC CCATTATGAC CACTAATGGG TATTTTACCA TCCCTCAGGC GTCTCTAGCT 
GGGCAGTGCC TACAGGATGC CCCTGTGGCT TTCCTGAGGA ATTTTCACAG TGTATGTACC 
ACGGACCTGG AAGTGCAAGA ACGAGATCGG CTTATCCCTG AAGACATGAG GATCCGCACG 
ACAGGAGGCC TGGTCACACC AACCGTAACC TATGAGGAAG CCACTGACCT GGACAAGCTC 
ATCACTAGTC CAGACACAAT TTTAAGTGCC GGATCGGCTC CCAGAAATGT GACTGTGGAA 
GAACATTATG TTTTCAGATG GCAAAATAAT TCTATCAGCA GCCTAGACAT CACAATCATC 
AGGGCAGAAA TCAACGCCCA CCAGAGAGGA ACAATGACCC AGAGATTTAC AGTGAAGTTT 
TTAAGCCCTA ACAGTGGTGG TGAGAAGGAA TTTTCTGGAA ATCCAGGTTA CCAGCTTGGC 
AAGCCGGTCC GAGCTCTGCA TACCGATGGC ATGAATGTCA CCACGTTGCA CCTCTGGCAG 
CCGGCTGGAA GGGGCCTGTG CACATCAGCC GCCCTCAGAC CTGTTTTGTT TGGAGAAGAT 
GCCTCCTCTG GCTGCCTTTT GGAAGTTGGG ATTAAGGAGA ACTGTACACA ACTCAGGGAG 
AATGTCCTTC AGAGACTAGA TTTGCTGATC CAGGCAACGC ACGTTGCGAG GAGAGGCAAC 
TCGGACTACG GTGATCTGAG CGATGGCTGG CTGGAGGTCA TACGCGTCGA CGCCCCTGAT 
GCAGGCGCCG ACCTGCCTCT GAGCAGCGCA AATGGCATGT GCCCGGAAGT TCCAACCCAC 
GTCACCATCC GCATCCTCAC TGCTGAGGCT GGCGCTGTGG AAGGGGAGGC TCAGCGGGAG 
ATCCTGGCTG TGGAGACAAG GTTCTCCACA GTAACCTGGC AGTACCAATG TGGGCTCACC 
TGTGAGGAGG ACAAAGCCGA CCTTCTCCCC CTCAGTGCCT CTGTTAAGTT CATTAACATC 
CCTGCGCAGA TGCCCCGCCC GACCAGATTC CAGATCAACT TCACAGAGTA CGATTGTACC 
CGGAATGAGC TGTGCTGGCC CCAACTTCTG TACCCTCTGA CCCAATATTA TCAAGGGGAG 
CCCCGGCCTC AGTGTGTCGC CAAAGGCCTG ATGCTGCTGT CACTCCTCAT GTTGGCCATA 
CTCCTCAGGC ACCCTTGGGT CGGGATGTGC AAAGCCTGGT CCTCCGCTTC CATCCAGCAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074251.1
CDS	73..2175
Translation	MGSLSPLSFLWGLLLLQGVLRPLRGDPVFIPPFIRMSSPEVRASLVGGSEDVTVSLTPLQIKEGVLPVPTCGGLRNETGDWNLTVSPQANMLEVTVRWKRGLDWCSPDETASFSEAPCIVQTLLVSASHNASCLAHLLIQVEIYPNTSVTHNASENMTVIPNQVYQPLGPCPCDLTAKACDIRCCCDQDCQPELRELFERFCFSGVFGGYVSPPSHHRCAARTTHQTPDWFPFLCVQSPPSTSPFLGHFYHGAISPRHSPGFETHSHLDLRDFFADASYKQGDPIMTTNGYFTIPQASLAGQCLQDAPVAFLRNFHSVCTTDLEVQERDRLIPEDMRIRTTGGLVTPTVTYEEATDLDKLITSPDTILSAGSAPRNVTVEEHYVFRWQNNSISSLDITIIRAEINAHQRGTMTQRFTVKFLSPNSGGEKEFSGNPGYQLGKPVRALHTDGMNVTTLHLWQPAGRGLCTSAALRPVLFGEDASSGCLLEVGIKENCTQLRENVLQRLDLLIQATHVARRGNSDYGDLSDGWLEVIRVDAPDAGADLPLSSANGMCPEVPTHVTIRILTAEAGAVEGEAQREILAVETRFSTVTWQYQCGLTCEEDKADLLPLSASVKFINIPAQMPRPTRFQINFTEYDCTRNELCWPQLLYPLTQYYQGEPRPQCVAKGLMLLSLLMLAILLRHPWVGMCKAWSSASIQH

Target ORF information:

RefSeq Version	NM_001080782.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tectonic family member 2 (Tctn2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001080782.1

ORF Insert Sequence:

ATGGGTTCTC TGTCGCCGCT CAGCTTCCTG TGGGGACTCC TCCTGCTGCA GGGCGTCCTG 
AGGCCGCTGC GCGGCGACCC GGTTTTCATC CCACCTTTCA TCCGGATGTC CAGTCCTGAG 
GTCCGCGCGT CCCTGGTCGG AGGCAGCGAG GATGTCACCG TGTCACTGAC TCCACTGCAA 
ATCAAGGAAG GAGTGTTGCC AGTTCCTACT TGTGGTGGCC TCAGGAATGA GACCGGGGAT 
TGGAATCTGA CTGTGAGCCC CCAAGCGAAC ATGCTGGAAG TGACCGTAAG GTGGAAGAGG 
GGTCTGGACT GGTGCTCTCC AGATGAGACA GCCTCGTTCT CAGAGGCCCC ATGCATCGTG 
CAGACTCTGC TGGTCTCAGC ATCCCACAAT GCATCCTGCT TAGCACATCT GCTCATCCAG 
GTGGAGATTT ATCCCAACAC GTCTGTGACC CACAATGCAT CAGAGAACAT GACTGTCATT 
CCTAACCAAG TCTATCAGCC CCTGGGTCCC TGCCCGTGTG ACTTGACAGC CAAGGCCTGT 
GACATTCGCT GCTGCTGTGA CCAAGACTGT CAGCCGGAGC TAAGAGAGCT GTTCGAACGG 
TTCTGCTTCT CCGGCGTGTT TGGCGGGTAC GTCAGCCCTC CGTCCCACCA TCGCTGTGCA 
GCCCGCACTA CTCACCAGAC TCCTGACTGG TTTCCCTTCC TGTGTGTGCA GTCACCACCC 
TCCACCTCTC CGTTCCTCGG TCACTTCTAC CACGGCGCCA TTTCCCCCAG ACATTCCCCC 
GGCTTTGAAA CCCATTCGCA CTTGGACCTA AGAGACTTCT TTGCTGACGC CAGCTACAAA 
CAAGGGGATC CCATTATGAC CACTAATGGG TATTTTACCA TCCCTCAGGC GTCTCTAGCT 
GGGCAGTGCC TACAGGATGC CCCTGTGGCT TTCCTGAGGA ATTTTCACAG TGTATGTACC 
ACGGACCTGG AAGTGCAAGA ACGAGATCGG CTTATCCCTG AAGACATGAG GATCCGCACG 
ACAGGAGGCC TGGTCACACC AACCGTAACC TATGAGGAAG CCACTGACCT GGACAAGCTC 
ATCACTAGTC CAGACACAAT TTTAAGTGCC GGATCGGCTC CCAGAAATGT GACTGTGGAA 
GAACATTATG TTTTCAGATG GCAAAATAAT TCTATCAGCA GCCTAGACAT CACAATCATC 
AGGGCAGAAA TCAACGCCCA CCAGAGAGGA ACAATGACCC AGAGATTTAC AGTGAAGTTT 
TTAAGCCCTA ACAGTGGTGG TGAGAAGGAA TTTTCTGGAA ATCCAGGTTA CCAGCTTGGC 
AAGCCGGTCC GAGCTCTGCA TACCGATGGC ATGAATGTCA CCACGTTGCA CCTCTGGCAG 
CCGGCTGGAA GGGGCCTGTG CACATCAGCC GCCCTCAGAC CTGTTTTGTT TGGAGAAGAT 
GCCTCCTCTG GCTGCCTTTT GGAAGTTGGG ATTAAGGAGA ACTGTACACA ACTCAGGGAG 
AATGTCCTTC AGAGACTAGA TTTGCTGATC CAGGCAACGC ACGTTGCGAG GAGAGGCAAC 
TCGGACTACG GTGATCTGAG CGATGGCTGG CTGGAGGTCA TACGCGTCGA CGCCCCTGAT 
GCAGGCGCCG ACCTGCCTCT GAGCAGCGCA AATGGCATGT GCCCGGAAGT TCCAACCCAC 
GTCACCATCC GCATCCTCAC TGCTGAGGCT GGCGCTGTGG AAGGGGAGGC TCAGCGGGAG 
ATCCTGGCTG TGGAGACAAG GTTCTCCACA GTAACCTGGC AGTACCAATG TGGGCTCACC 
TGTGAGGAGG ACAAAGCCGA CCTTCTCCCC CTCAGTGCCT CTGTTAAGTT CATTAACATC 
CCTGCGCAGA TGCCCCGCCC GACCAGATTC CAGATCAACT TCACAGAGTA CGATTGTACC 
CGGAATGAGC TGTGCTGGCC CCAACTTCTG TACCCTCTGA CCCAATATTA TCAAGGGGAG 
CCCCGGCCTC AGTGTGTCGC CAAAGGCCTG ATGCTGCTGT CACTCCTCAT GTTGGCCATA 
CTCCTCAGGC ACCCTTGGGT CGGGATGTGC AAAGCCTGGT CCTCCGCTTC CATCCAGCAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tctn2 ( XM_008769263.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008767485.1 Rattus norvegicus tectonic family member 2 (Tctn2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa50392
Clone ID Related Accession (Same CDS sequence)	XM_008769263.2
Accession Version	XM_008769263.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2025bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	tectonic-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008769263.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCTC TGTCGCCGCT CAGCTTCCTG TGGGGACTCC TCCTGCTGCA GGGCGTCCTG 
AGGCCGCTGC GCGGCGACCC GGTTTTCATC CCACCTTTCA TCCGGATGTC CAGTCCTGAG 
GTCCGCGCGT CCCTGGTCGG AGGCAGCGAG GATGTCACCG TGTCACTGAC TCCACTGCAA 
ATCAAGGAAG GAGTGTTGCC AGTTCCTACT TGTGGTGGCC TCAGGAATGA GACCGGGGAT 
TGGAATCTGA CTGTGAGCCC CCAAGCGAAC ATGCTGGAAG TGACCGTAAG GTGGAAGAGG 
GGTCTGGACT GGTGCTCTCC AGATGAGACA GCCTCGTTCT CAGAGGCCCC ATGCATCGTG 
CAGACTCTGC TGGTCTCAGC ATCCCACAAT GCATCCTGCT TAGCACATCT GCTCATCCAG 
GTGGAGATTT ATCCCAACAC GTCTGTGACC CACAATGCAT CAGAGAACAT GACTGTCATT 
CCTAACCAAG TCTATCAGCC CCTGGGTCCC TGCCCGTGTG ACTTGACAGC CAAGGCCTGT 
GACATTCGCT GCTGCTGTGA CCAAGACTGT CAGCCGGAGC TAAGAGAGCT GTTCGAACGG 
TTCTGCTTCT CCGGCGTGTT TGGCGGGTAC GTCAGCCCTC CGTCCCACCA TCGCTGTGCA 
GCCCGCACTA CTCACCAGAC TCCTGACTGG TTTCCCTTCC TGTGTGTGCA GTCACCACCC 
TCCACCTCTC CGTTCCTCGG TCACTTCTAC CACGGCGCCA TTTCCCCCAG ACATTCCCCC 
GGCTTTGAAA CCCATTCGCA CTTGGACCTA AGAGACTTCT TTGCTGACGC CAGCTACAAA 
CAAGGGGATC CCATTATGAC CACTAATGGG TATTTTACCA TCCCTCAGGC GTCTCTAGCT 
GGGCAGTGCC TACAGGATGC CCCTGTGGCT TTCCTGAGGA ATTTTCACAG TGTATGTACC 
ACGGACCTGG AAGTGCAAGA ACGAGATCGG CTTATCCCTG AAGACATGAG GATCCGCACG 
ACAGGAGGCC TGGTCACACC AACCGTAACC TATGAGGAAG CCACTGACCT GGACAAGCTC 
ATCACTAGTC CAGACACAAT TTTAAGTGCC GGATCGGCTC CCAGAAATGT GACTGTGGAA 
GAACATTATG TTTTCAGATG GCAAAATAAT TCTATCAGCA GCCTAGACAT CACAATCATC 
AGGGCAGAAA TCAACGCCCA CCAGAGAGGA ACAATGACCC AGAGATTTAC AGTGAAGTTT 
TTAAGCCCTA ACAGTGGTGG TGAGAAGGAA TTTTCTGGAA ATCCAGCTGG AAGGGGCCTG 
TGCACATCAG CCGCCCTCAG ACCTGTTTTG TTTGGAGAAG ATGCCTCCTC TGGCTGCCTT 
TTGGAAGTTG GGATTAAGGA GAACTGTACA CAACTCAGGG AGAATGTCCT TCAGAGACTA 
GATTTGCTGA TCCAGGCAAC GCACGTTGCG AGGAGAGGCA ACTCGGACTA CGGTGATCTG 
AGCGATGGCT GGCTGGAGGT CATACGCGTC GACGCCCCTG ATGCAGGCGC CGACCTGCCT 
CTGAGCAGCG CAAATGGCAT GTGCCCGGAA GTTCCAACCC ACGTCACCAT CCGCATCCTC 
ACTGCTGAGG CTGGCGCTGT GGAAGGGGAG GCTCAGCGGG AGATCCTGGC TGTGGAGACA 
AGGTTCTCCA CAGTAACCTG GCAGTACCAA TGTGGGCTCA CCTGTGAGGA GGACAAAGCC 
GACCTTCTCC CCCTCAGTGC CTCTGTTAAG TTCATTAACA TCCCTGCGCA GATGCCCCGC 
CCGACCAGAT TCCAGATCAA CTTCACAGAG TACGATTGTA CCCGGAATGA GCTGTGCTGG 
CCCCAACTTC TGTACCCTCT GACCCAATAT TATCAAGGGG AGCCCCGGCC TCAGTGTGTC 
GCCAAAGGCC TGATGCTGCT GTCACTCCTC ATGTTGGCCA TACTCCTCAG GCACCCTTGG 
GTCGGGATGT GCAAAGCCTG GTCCTCCGCT TCCATCCAGC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008767485.1
CDS	1733..3757
Translation	MGSLSPLSFLWGLLLLQGVLRPLRGDPVFIPPFIRMSSPEVRASLVGGSEDVTVSLTPLQIKEGVLPVPTCGGLRNETGDWNLTVSPQANMLEVTVRWKRGLDWCSPDETASFSEAPCIVQTLLVSASHNASCLAHLLIQVEIYPNTSVTHNASENMTVIPNQVYQPLGPCPCDLTAKACDIRCCCDQDCQPELRELFERFCFSGVFGGYVSPPSHHRCAARTTHQTPDWFPFLCVQSPPSTSPFLGHFYHGAISPRHSPGFETHSHLDLRDFFADASYKQGDPIMTTNGYFTIPQASLAGQCLQDAPVAFLRNFHSVCTTDLEVQERDRLIPEDMRIRTTGGLVTPTVTYEEATDLDKLITSPDTILSAGSAPRNVTVEEHYVFRWQNNSISSLDITIIRAEINAHQRGTMTQRFTVKFLSPNSGGEKEFSGNPAGRGLCTSAALRPVLFGEDASSGCLLEVGIKENCTQLRENVLQRLDLLIQATHVARRGNSDYGDLSDGWLEVIRVDAPDAGADLPLSSANGMCPEVPTHVTIRILTAEAGAVEGEAQREILAVETRFSTVTWQYQCGLTCEEDKADLLPLSASVKFINIPAQMPRPTRFQINFTEYDCTRNELCWPQLLYPLTQYYQGEPRPQCVAKGLMLLSLLMLAILLRHPWVGMCKAWSSASIQH

Target ORF information:

RefSeq Version	XM_008769263.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tectonic family member 2 (Tctn2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008769263.2

ORF Insert Sequence:

ATGGGTTCTC TGTCGCCGCT CAGCTTCCTG TGGGGACTCC TCCTGCTGCA GGGCGTCCTG 
AGGCCGCTGC GCGGCGACCC GGTTTTCATC CCACCTTTCA TCCGGATGTC CAGTCCTGAG 
GTCCGCGCGT CCCTGGTCGG AGGCAGCGAG GATGTCACCG TGTCACTGAC TCCACTGCAA 
ATCAAGGAAG GAGTGTTGCC AGTTCCTACT TGTGGTGGCC TCAGGAATGA GACCGGGGAT 
TGGAATCTGA CTGTGAGCCC CCAAGCGAAC ATGCTGGAAG TGACCGTAAG GTGGAAGAGG 
GGTCTGGACT GGTGCTCTCC AGATGAGACA GCCTCGTTCT CAGAGGCCCC ATGCATCGTG 
CAGACTCTGC TGGTCTCAGC ATCCCACAAT GCATCCTGCT TAGCACATCT GCTCATCCAG 
GTGGAGATTT ATCCCAACAC GTCTGTGACC CACAATGCAT CAGAGAACAT GACTGTCATT 
CCTAACCAAG TCTATCAGCC CCTGGGTCCC TGCCCGTGTG ACTTGACAGC CAAGGCCTGT 
GACATTCGCT GCTGCTGTGA CCAAGACTGT CAGCCGGAGC TAAGAGAGCT GTTCGAACGG 
TTCTGCTTCT CCGGCGTGTT TGGCGGGTAC GTCAGCCCTC CGTCCCACCA TCGCTGTGCA 
GCCCGCACTA CTCACCAGAC TCCTGACTGG TTTCCCTTCC TGTGTGTGCA GTCACCACCC 
TCCACCTCTC CGTTCCTCGG TCACTTCTAC CACGGCGCCA TTTCCCCCAG ACATTCCCCC 
GGCTTTGAAA CCCATTCGCA CTTGGACCTA AGAGACTTCT TTGCTGACGC CAGCTACAAA 
CAAGGGGATC CCATTATGAC CACTAATGGG TATTTTACCA TCCCTCAGGC GTCTCTAGCT 
GGGCAGTGCC TACAGGATGC CCCTGTGGCT TTCCTGAGGA ATTTTCACAG TGTATGTACC 
ACGGACCTGG AAGTGCAAGA ACGAGATCGG CTTATCCCTG AAGACATGAG GATCCGCACG 
ACAGGAGGCC TGGTCACACC AACCGTAACC TATGAGGAAG CCACTGACCT GGACAAGCTC 
ATCACTAGTC CAGACACAAT TTTAAGTGCC GGATCGGCTC CCAGAAATGT GACTGTGGAA 
GAACATTATG TTTTCAGATG GCAAAATAAT TCTATCAGCA GCCTAGACAT CACAATCATC 
AGGGCAGAAA TCAACGCCCA CCAGAGAGGA ACAATGACCC AGAGATTTAC AGTGAAGTTT 
TTAAGCCCTA ACAGTGGTGG TGAGAAGGAA TTTTCTGGAA ATCCAGCTGG AAGGGGCCTG 
TGCACATCAG CCGCCCTCAG ACCTGTTTTG TTTGGAGAAG ATGCCTCCTC TGGCTGCCTT 
TTGGAAGTTG GGATTAAGGA GAACTGTACA CAACTCAGGG AGAATGTCCT TCAGAGACTA 
GATTTGCTGA TCCAGGCAAC GCACGTTGCG AGGAGAGGCA ACTCGGACTA CGGTGATCTG 
AGCGATGGCT GGCTGGAGGT CATACGCGTC GACGCCCCTG ATGCAGGCGC CGACCTGCCT 
CTGAGCAGCG CAAATGGCAT GTGCCCGGAA GTTCCAACCC ACGTCACCAT CCGCATCCTC 
ACTGCTGAGG CTGGCGCTGT GGAAGGGGAG GCTCAGCGGG AGATCCTGGC TGTGGAGACA 
AGGTTCTCCA CAGTAACCTG GCAGTACCAA TGTGGGCTCA CCTGTGAGGA GGACAAAGCC 
GACCTTCTCC CCCTCAGTGC CTCTGTTAAG TTCATTAACA TCCCTGCGCA GATGCCCCGC 
CCGACCAGAT TCCAGATCAA CTTCACAGAG TACGATTGTA CCCGGAATGA GCTGTGCTGG 
CCCCAACTTC TGTACCCTCT GACCCAATAT TATCAAGGGG AGCCCCGGCC TCAGTGTGTC 
GCCAAAGGCC TGATGCTGCT GTCACTCCTC ATGTTGGCCA TACTCCTCAG GCACCCTTGG 
GTCGGGATGT GCAAAGCCTG GTCCTCCGCT TCCATCCAGC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MKS5 and CEP290 Dependent Assembly Pathway of the Ciliary Transition Zone.
PLoS biology14(3)e1002416(2016 Mar)
Li C,Jensen VL,Park K,Kennedy J,Garcia-Gonzalo FR,Romani M,De Mori R,Bruel AL,Gaillard D,Doray B,Lopez E,Rivière JB,Faivre L,Thauvin-Robinet C,Reiter JF,Blacque OE,Valente EM,Leroux MR

A ciliopathy complex at the transition zone protects the cilia as a privileged membrane domain.
Nature cell biology14(1)61-72(2011 Dec)
Chih B,Liu P,Chinn Y,Chalouni C,Komuves LG,Hass PE,Sandoval W,Peterson AS

A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition.
Nature genetics43(8)776-84(2011 Jul)
Garcia-Gonzalo FR,Corbit KC,Sirerol-Piquer MS,Ramaswami G,Otto EA,Noriega TR,Seol AD,Robinson JF,Bennett CL,Josifova DJ,García-Verdugo JM,Katsanis N,Hildebrandt F,Reiter JF

Mapping the NPHP-JBTS-MKS protein network reveals ciliopathy disease genes and pathways.
Cell145(4)513-28(2011 May)
Sang L,Miller JJ,Corbit KC,Giles RH,Brauer MJ,Otto EA,Baye LM,Wen X,Scales SJ,Kwong M,Huntzicker EG,Sfakianos MK,Sandoval W,Bazan JF,Kulkarni P,Garcia-Gonzalo FR,Seol AD,O'Toole JF,Held S,Reutter HM,Lane WS,Rafiq MA,Noor A,Ansar M,Devi AR,Sheffield VC,Slusarski DC,Vincent JB,Doherty DA,Hildebrandt F,Reiter JF,Jackson PK

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