Kcnmb4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Kcnmb4
Entrez Gene ID	66016
Full Name	potassium calcium-activated channel subfamily M regulatory beta subunit 4
General protein information	Preferred Names potassium calcium-activated channel subfamily M regulatory beta subunit 4 Names calcium-activated potassium channel subunit beta-4 BK channel subunit beta-4 BKbeta4 calcium activated potassium channel beta 4 subunit calcium-activated potassium channel, subfamily M subunit beta-4 charybdotoxin receptor subunit beta-4 k(VCA)beta-4 maxi K channel subunit beta-4 potassium channel subfamily M regulatory beta subunit 4 potassium large conductance calcium-activated channel, subfamily M, beta member 4 slo-beta-4
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q22
Summary	mouse homolog is a subunit of the large conductance calcium-dependent potassium channel; downregulates channel activity [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_023960.2	NP_076450.1	calcium-activated potassium channel subunit beta-4

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	KCNMB4	NP_001186559.1
Homo sapiens (human)	KCNMB4	NP_055320.4
Mus musculus (house mouse)	Kcnmb4	NP_067427.1
Macaca mulatta (Rhesus monkey)	LOC717360	XP_002798706.1
Canis lupus familiaris (dog)	KCNMB4	XP_005625718.1
Xenopus tropicalis (tropical clawed frog)	kcnmb4	NP_001017211.1
Pan troglodytes (chimpanzee)	KCNMB4	XP_001156630.1
Bos taurus (cattle)	KCNMB4	NP_001179452.1
Rattus norvegicus (Norway rat)	Kcnmb4	NP_076450.1

Gene Ontology
Bibliography

Related articles in PubMed

Impairment of brain mitochondrial charybdotoxin- and ATP-insensitive BK channel activities in diabetes.
Noursadeghi E, Jafari A, Saghiri R, Sauve R, Eliassi A
Neuromolecular medicine16(4)862-71(2014 Dec)

An endoplasmic reticulum trafficking signal regulates surface expression of β4 subunit of a voltage- and Ca²⁺-activated K⁺ channel.
Cox N, Toro B, Pacheco-Otalora LF, Garrido-Sanabria ER, Zarei MM
Brain research155312-23(2014 Mar)

Modulation by the BK accessory β4 subunit of phosphorylation-dependent changes in excitability of dentate gyrus granule neurons.
Petrik D, Wang B, Brenner R
The European journal of neuroscience34(5)695-704(2011 Sep)

Expression of the voltage- and Ca2+-dependent BK potassium channel subunits BKβ1 and BKβ4 in rodent astrocytes.
Seidel KN, Derst C, Salzmann M, Höltje M, Priller J, Markgraf R, Heinemann SH, Heilmann H, Skatchkov SN, Eaton MJ, Veh RW, Prüss H
Glia59(6)893-902(2011 Jun)

Compartmentalized beta subunit distribution determines characteristics and ethanol sensitivity of somatic, dendritic, and terminal large-conductance calcium-activated potassium channels in the rat central nervous system.
Wynne PM, Puig SI, Martin GE, Treistman SN
The Journal of pharmacology and experimental therapeutics329(3)978-86(2009 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Kcnmb4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Kcnmb4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa42705	NM_023960.2 Latest version!	Rattus norvegicus potassium calcium-activated channel subfamily M regulatory beta subunit 4 (Kcnmb4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Kcnmb4 ( NM_023960.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_076450.1 Rattus norvegicus potassium calcium-activated channel subfamily M regulatory beta subunit 4 (Kcnmb4), mRNA.

Price & Availability↑

CloneID	ORa42705
Clone ID Related Accession (Same CDS sequence)	NM_023960.2
Accession Version	NM_023960.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 633bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577289600000
Organism	Rattus norvegicus(Norway rat)
Product	calcium-activated potassium channel subunit beta-4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY028605.1. On Jun 9, 2003 this sequence version replaced NM_023960.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY028605.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906348 [ECO:0000348] ##Evidence-Data-END##

ATGGCGAAGC TCAGGGTGTC TTACGAGTAC ACGGAAGCCG AAGACAAGAG CATCCGGCTC 
GGCTTGTTCC TCATCGTCTC TGGCATCCTG TCGCTCTTCA TCTTCGGCTT CTGCTGGCTC 
AGTCCCGCCT TGCAGGATCT GCAAGCCACG GCGGCCAACT GCACGGTGCT GTCGGTGCAG 
CAGATCGGCG AGGTGTTCGA GTGCACCTTC ACCTGTGGCA CCGACTGCAG GGGCACCTCG 
CAGTATCCCT GCGTCCAGGT GTACGTGAAC AACTCCGAGT CCAACTCCAG GGCGCTGCTA 
CACAGCGACC AGCACCAGCT CCTGACTAAC CCCAAGTGCT CCTATATCCC GCCCTGTAAG 
AGAGAAAACC AGAAGAACTC GGAAAGTGTC ATGAACTGGC AACAGTATTG GAAAGATGAG 
ATCGGTTCCC AGCCATTCAC TTGCTATTTT AATCAACATC AAAGACCAGA GGACGTTCTC 
CTGCAGCGCA CACATGACGA GATTGTCCTC CTGCACTGCT TCCTCTGGCC GGTGGTGGCG 
TTTGTGGTGG GTGTCCTCAT CGTGGTCCTC ACCATCTGTG CCAAGAGCCT GGCAGTCAAG 
GCAGAAGCCA TGAAGAAACG CAAGTTCTCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_076450.1
CDS	19..651
Translation	MAKLRVSYEYTEAEDKSIRLGLFLIVSGILSLFIFGFCWLSPALQDLQATAANCTVLSVQQIGEVFECTFTCGTDCRGTSQYPCVQVYVNNSESNSRALLHSDQHQLLTNPKCSYIPPCKRENQKNSESVMNWQQYWKDEIGSQPFTCYFNQHQRPEDVLLQRTHDEIVLLHCFLWPVVAFVVGVLIVVLTICAKSLAVKAEAMKKRKFS

Target ORF information:

RefSeq Version	NM_023960.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus potassium calcium-activated channel subfamily M regulatory beta subunit 4 (Kcnmb4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_023960.2

ORF Insert Sequence:

ATGGCGAAGC TCAGGGTGTC TTACGAGTAC ACGGAAGCCG AAGACAAGAG CATCCGGCTC 
GGCTTGTTCC TCATCGTCTC TGGCATCCTG TCGCTCTTCA TCTTCGGCTT CTGCTGGCTC 
AGTCCCGCCT TGCAGGATCT GCAAGCCACG GCGGCCAACT GCACGGTGCT GTCGGTGCAG 
CAGATCGGCG AGGTGTTCGA GTGCACCTTC ACCTGTGGCA CCGACTGCAG GGGCACCTCG 
CAGTATCCCT GCGTCCAGGT GTACGTGAAC AACTCCGAGT CCAACTCCAG GGCGCTGCTA 
CACAGCGACC AGCACCAGCT CCTGACTAAC CCCAAGTGCT CCTATATCCC GCCCTGTAAG 
AGAGAAAACC AGAAGAACTC GGAAAGTGTC ATGAACTGGC AACAGTATTG GAAAGATGAG 
ATCGGTTCCC AGCCATTCAC TTGCTATTTT AATCAACATC AAAGACCAGA GGACGTTCTC 
CTGCAGCGCA CACATGACGA GATTGTCCTC CTGCACTGCT TCCTCTGGCC GGTGGTGGCG 
TTTGTGGTGG GTGTCCTCAT CGTGGTCCTC ACCATCTGTG CCAAGAGCCT GGCAGTCAAG 
GCAGAAGCCA TGAAGAAACG CAAGTTCTCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Impairment of brain mitochondrial charybdotoxin- and ATP-insensitive BK channel activities in diabetes.
Neuromolecular medicine16(4)862-71(2014 Dec)
Noursadeghi E,Jafari A,Saghiri R,Sauve R,Eliassi A

An endoplasmic reticulum trafficking signal regulates surface expression of β4 subunit of a voltage- and Ca²⁺-activated K⁺ channel.
Brain research155312-23(2014 Mar)
Cox N,Toro B,Pacheco-Otalora LF,Garrido-Sanabria ER,Zarei MM

Modulation by the BK accessory β4 subunit of phosphorylation-dependent changes in excitability of dentate gyrus granule neurons.
The European journal of neuroscience34(5)695-704(2011 Sep)
Petrik D,Wang B,Brenner R

Expression of the voltage- and Ca2+-dependent BK potassium channel subunits BKβ1 and BKβ4 in rodent astrocytes.
Glia59(6)893-902(2011 Jun)
Seidel KN,Derst C,Salzmann M,Höltje M,Priller J,Markgraf R,Heinemann SH,Heilmann H,Skatchkov SN,Eaton MJ,Veh RW,Prüss H

Compartmentalized beta subunit distribution determines characteristics and ethanol sensitivity of somatic, dendritic, and terminal large-conductance calcium-activated potassium channels in the rat central nervous system.
The Journal of pharmacology and experimental therapeutics329(3)978-86(2009 Jun)
Wynne PM,Puig SI,Martin GE,Treistman SN