Barhl2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Barhl2
Entrez Gene ID	65050
Full Name	BarH-like homeobox 2
Synonyms	Barh,MBH1
General protein information	Preferred Names BarH-like homeobox 2 Names barH-like 2 homeobox protein BarH-class homeodomain transcription factor bar-class homeodomain protein MBH1 homeobox protein B-H1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 14 Map Location: 14p22
Summary	potential regulator of mammalian neural bHLH genes [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_022956.2	NP_075245.1	barH-like 2 homeobox protein

Pathways from BioSystems

Homologies

Bos taurus (cattle)	BARHL2	NP_001179226.1
Pan troglodytes (chimpanzee)	BARHL2	XP_003949506.1
Mus musculus (house mouse)	Barhl2	NP_001005477.1
Canis lupus familiaris (dog)	BARHL2	XP_853271.3
Rattus norvegicus (Norway rat)	Barhl2	NP_075245.1
Homo sapiens (human)	BARHL2	NP_064447.1
Macaca mulatta (Rhesus monkey)	BARHL2	XP_001089467.1
Danio rerio (zebrafish)	barhl2	NP_991303.1
Xenopus tropicalis (tropical clawed frog)	barhl2	XP_002940151.1

Gene Ontology
Bibliography

Related articles in PubMed

Up-regulation of miR-187 modulates the advances of oral carcinoma by targeting BARX2 tumor suppressor.
Lin SC, Kao SY, Chang JC, Liu YC, Yu EH, Tseng SH, Liu CJ, Chang KW
Oncotarget7(38)61355-61365(2016 09)

Down-regulation of Barx2 predicts poor survival in colorectal cancer.
Mi Y, Zhao S, Zhang W, Zhang D, Weng J, Huang K, Sun H, Tang H, Zhang X, Sun X, Peng Z, Wen Y
Biochemical and biophysical research communications478(1)67-73(2016 09)

BARHL2 Methylation Using Gastric Wash DNA or Gastric Juice Exosomal DNA is a Useful Marker For Early Detection of Gastric Cancer in an H. pylori-Independent Manner.
Yamamoto H, Watanabe Y, Oikawa R, Morita R, Yoshida Y, Maehata T, Yasuda H, Itoh F
Clinical and translational gastroenterology7(7)e184(2016 Jul)

Barhl1 regulatory sequences required for cell-specific gene expression and autoregulation in the inner ear and central nervous system.
Chellappa R, Li S, Pauley S, Jahan I, Jin K, Xiang M
Molecular and cellular biology28(6)1905-14(2008 Mar)

Role of the Barhl2 homeobox gene in the specification of glycinergic amacrine cells.
Mo Z, Li S, Yang X, Xiang M
Development (Cambridge, England)131(7)1607-18(2004 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Barhl2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Barhl2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa06537	NM_022956.2 Latest version!	Rattus norvegicus BarH-like homeobox 2 (Barhl2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Barhl2 ( NM_022956.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_075245.1 Rattus norvegicus BarH-like homeobox 2 (Barhl2), mRNA.

Price & Availability↑

CloneID	ORa06537
Clone ID Related Accession (Same CDS sequence)	NM_022956.2
Accession Version	NM_022956.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571328000000
Organism	Rattus norvegicus(Norway rat)
Product	barH-like 2 homeobox protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB004056.1. On Aug 31, 2012 this sequence version replaced NM_022956.1. ##Evidence-Data-START## Transcript exon combination :: AB004056.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGACAGCAA TGGAAGGGGC CAGCGGGTCG AGTTTTGGAA TAGACACGAT TTTGTCCGGT 
GCCGGTTCCG GCAGTCCGGG CATGATGAAC GGAGATTTCC GATCGCTCGG TGAAGCGAGG 
ACCACGGATT TTAGGAGCCA GGCCACCCCG TCACCCTGTT CGGAGATTGA TACCGTGGGA 
ACGGCGCCCT CTTCACCGAT CTCGGTCACC CTGGAGCCCC CGGAGCCGCA TCTTGTGACA 
GACGGACCCC AGCATCACCA TCACCTCCAC CACGGCCAAC AGCCACCGCC ACCGTCCGCG 
CCCCCCGCGC AAAGTTTGCA GCCTTCTCCC CAACAGCAAC CGCCGCCGCA GCCGCAGTCT 
GCAGCCCAGC AACTGGGCTC GGCCGCCGCG GCCCCCAGGA CTTCCACCTC TTCCTTTTTA 
ATTAAGGACA TCTTGGGAGA CAGCAAACCC CTGGCGGCTT GTGCACCCTA CAGCACCAGC 
GTTTCTTCTC CTCATCACAC CCCGAAGCAG GAGTGCAACG CGGCGCACGA GAGCTTCAGG 
CCAAAGCTGG AGCAGGAGGA CAGCAAAACC AAGCTGGACA AGAGGGAAGA CTCTCAGAGC 
GACATCAAAT GCCACGGAAC AAAGGAGGAA GGAGACCGGG AGATTACAAG TAGCCGAGAG 
AGTCCCCCTG TGAGAGCCAA GAAACCTCGA AAGGCCAGGA CAGCTTTCTC GGACCACCAG 
CTCAATCAAC TGGAACGTAG CTTTGAGCGG CAGAAGTACC TCAGTGTACA GGACCGCATG 
GACCTGGCAG CTGCACTCAA CCTCACTGAT ACCCAAGTCA AGACCTGGTA CCAGAACCGC 
AGAACCAAGT GGAAGCGGCA GACTGCAGTG GGCCTGGAGT TGCTTGCCGA GGCGGGGAAC 
TACTCCGCGC TGCAGAGGAT GTTTCCGTCG CCTTATTTCT ACCACCCAAG CCTGCTGGGG 
AGCATGGACA GCACGACGGC GGCCGCGGCG GCCGCGGCAA TGTACAGCAG CATGTACCGA 
ACTCCTCCTG CACCCCATCC GCAGCTGCAG CGGCCACTGG TGCCCCGCGT GCTCATCCAC 
GGCCTGGGGC CTGGGGGACA GCCGGCCCTC AACCCTTTGT CCAACCCCAT CCCAGGCACC 
CCGCATCCCC GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_075245.1
CDS	70..1224
Translation	MTAMEGASGSSFGIDTILSGAGSGSPGMMNGDFRSLGEARTTDFRSQATPSPCSEIDTVGTAPSSPISVTLEPPEPHLVTDGPQHHHHLHHGQQPPPPSAPPAQSLQPSPQQQPPPQPQSAAQQLGSAAAAPRTSTSSFLIKDILGDSKPLAACAPYSTSVSSPHHTPKQECNAAHESFRPKLEQEDSKTKLDKREDSQSDIKCHGTKEEGDREITSSRESPPVRAKKPRKARTAFSDHQLNQLERSFERQKYLSVQDRMDLAAALNLTDTQVKTWYQNRRTKWKRQTAVGLELLAEAGNYSALQRMFPSPYFYHPSLLGSMDSTTAAAAAAAMYSSMYRTPPAPHPQLQRPLVPRVLIHGLGPGGQPALNPLSNPIPGTPHPR

Target ORF information:

RefSeq Version	NM_022956.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus BarH-like homeobox 2 (Barhl2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022956.2

ORF Insert Sequence:

ATGACAGCAA TGGAAGGGGC CAGCGGGTCG AGTTTTGGAA TAGACACGAT TTTGTCCGGT 
GCCGGTTCCG GCAGTCCGGG CATGATGAAC GGAGATTTCC GATCGCTCGG TGAAGCGAGG 
ACCACGGATT TTAGGAGCCA GGCCACCCCG TCACCCTGTT CGGAGATTGA TACCGTGGGA 
ACGGCGCCCT CTTCACCGAT CTCGGTCACC CTGGAGCCCC CGGAGCCGCA TCTTGTGACA 
GACGGACCCC AGCATCACCA TCACCTCCAC CACGGCCAAC AGCCACCGCC ACCGTCCGCG 
CCCCCCGCGC AAAGTTTGCA GCCTTCTCCC CAACAGCAAC CGCCGCCGCA GCCGCAGTCT 
GCAGCCCAGC AACTGGGCTC GGCCGCCGCG GCCCCCAGGA CTTCCACCTC TTCCTTTTTA 
ATTAAGGACA TCTTGGGAGA CAGCAAACCC CTGGCGGCTT GTGCACCCTA CAGCACCAGC 
GTTTCTTCTC CTCATCACAC CCCGAAGCAG GAGTGCAACG CGGCGCACGA GAGCTTCAGG 
CCAAAGCTGG AGCAGGAGGA CAGCAAAACC AAGCTGGACA AGAGGGAAGA CTCTCAGAGC 
GACATCAAAT GCCACGGAAC AAAGGAGGAA GGAGACCGGG AGATTACAAG TAGCCGAGAG 
AGTCCCCCTG TGAGAGCCAA GAAACCTCGA AAGGCCAGGA CAGCTTTCTC GGACCACCAG 
CTCAATCAAC TGGAACGTAG CTTTGAGCGG CAGAAGTACC TCAGTGTACA GGACCGCATG 
GACCTGGCAG CTGCACTCAA CCTCACTGAT ACCCAAGTCA AGACCTGGTA CCAGAACCGC 
AGAACCAAGT GGAAGCGGCA GACTGCAGTG GGCCTGGAGT TGCTTGCCGA GGCGGGGAAC 
TACTCCGCGC TGCAGAGGAT GTTTCCGTCG CCTTATTTCT ACCACCCAAG CCTGCTGGGG 
AGCATGGACA GCACGACGGC GGCCGCGGCG GCCGCGGCAA TGTACAGCAG CATGTACCGA 
ACTCCTCCTG CACCCCATCC GCAGCTGCAG CGGCCACTGG TGCCCCGCGT GCTCATCCAC 
GGCCTGGGGC CTGGGGGACA GCCGGCCCTC AACCCTTTGT CCAACCCCAT CCCAGGCACC 
CCGCATCCCC GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Up-regulation of miR-187 modulates the advances of oral carcinoma by targeting BARX2 tumor suppressor.
Oncotarget7(38)61355-61365(2016 09)
Lin SC,Kao SY,Chang JC,Liu YC,Yu EH,Tseng SH,Liu CJ,Chang KW

Down-regulation of Barx2 predicts poor survival in colorectal cancer.
Biochemical and biophysical research communications478(1)67-73(2016 09)
Mi Y,Zhao S,Zhang W,Zhang D,Weng J,Huang K,Sun H,Tang H,Zhang X,Sun X,Peng Z,Wen Y

BARHL2 Methylation Using Gastric Wash DNA or Gastric Juice Exosomal DNA is a Useful Marker For Early Detection of Gastric Cancer in an H. pylori-Independent Manner.
Clinical and translational gastroenterology7(7)e184(2016 Jul)
Yamamoto H,Watanabe Y,Oikawa R,Morita R,Yoshida Y,Maehata T,Yasuda H,Itoh F

Barhl1 regulatory sequences required for cell-specific gene expression and autoregulation in the inner ear and central nervous system.
Molecular and cellular biology28(6)1905-14(2008 Mar)
Chellappa R,Li S,Pauley S,Jahan I,Jin K,Xiang M

Role of the Barhl2 homeobox gene in the specification of glycinergic amacrine cells.
Development (Cambridge, England)131(7)1607-18(2004 Apr)
Mo Z,Li S,Yang X,Xiang M