Gphn cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gphn
Entrez Gene ID	64845
Full Name	gephyrin
Synonyms	Geph
General protein information	Preferred Names gephyrin Names gephyrin gephyrin isoform putative glycine receptor-tubulin linker protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q24
Summary	plays a role in molybdenum cofactor biosynthesis; may play a role in membrane protein-cytoskeleton interactions [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_022865.3	NP_074056.2	gephyrin

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GPHN	XP_001135362.2
Gallus gallus (chicken)	GPHN	NP_001026720.1
Danio rerio (zebrafish)	gphna	NP_001170915.1
Xenopus tropicalis (tropical clawed frog)	gphn	NP_001096372.1
Neurospora crassa	NCU09746	XP_964448.2
Rattus norvegicus (Norway rat)	Gphn	NP_074056.2
Magnaporthe oryzae (rice blast fungus)	MGG_06109	XP_003711975.1
Bos taurus (cattle)	GPHN	XP_005212135.1
Drosophila melanogaster (fruit fly)	cin	NP_726659.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002800	XP_312114.5
Homo sapiens (human)	GPHN	NP_065857.1
Macaca mulatta (Rhesus monkey)	GPHN	XP_002805147.1
Canis lupus familiaris (dog)	GPHN	XP_005623587.1
Mus musculus (house mouse)	Gphn	NP_666077.2
Danio rerio (zebrafish)	gphnb	NP_001165871.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequences Flanking the Gephyrin-Binding Site of GlyRβ Tune Receptor Stabilization at Synapses.
Grünewald N, Jan A, Salvatico C, Kress V, Renner M, Triller A, Specht CG, Schwarz G
eNeuro5(1)(2018 Jan-Feb)

Activity-Dependent Inhibitory Synapse Scaling Is Determined by Gephyrin Phosphorylation and Subsequent Regulation of GABA
Battaglia S, Renner M, Russeau M, Côme E, Tyagarajan SK, Lévi S
eNeuro5(1)(2018 Jan-Feb)

Endosomal Phosphatidylinositol 3-Phosphate Promotes Gephyrin Clustering and GABAergic Neurotransmission at Inhibitory Postsynapses.
Papadopoulos T, Rhee HJ, Subramanian D, Paraskevopoulou F, Mueller R, Schultz C, Brose N, Rhee JS, Betz H
The Journal of biological chemistry292(4)1160-1177(2017 01)

Expression of glycine receptors and gephyrin in rat medial vestibular nuclei and flocculi following unilateral labyrinthectomy.
Zhou W, Zhou LQ, Shi H, Leng YM, Liu B, Zhang SL, Kong WJ
International journal of molecular medicine38(5)1481-1489(2016 Nov)

Identification of an elaborate complex mediating postsynaptic inhibition.
Uezu A, Kanak DJ, Bradshaw TW, Soderblom EJ, Catavero CM, Burette AC, Weinberg RJ, Soderling SH
Science (New York, N.Y.)353(6304)1123-9(2016 09)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Gphn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gphn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13313	NM_022865.3 Latest version!	Rattus norvegicus gephyrin (Gphn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gphn ( NM_022865.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_074056.2 Rattus norvegicus gephyrin (Gphn), mRNA.

Price & Availability↑

CloneID	ORa13313
Clone ID Related Accession (Same CDS sequence)	NM_022865.3
Accession Version	NM_022865.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1568908800000
Organism	Rattus norvegicus(Norway rat)
Product	gephyrin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X66366.2. On Apr 20, 2007 this sequence version replaced NM_022865.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X66366.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCGACCG AGGGAATGAT CCTCACAAAC CACGACCATC AAATCCGTGT CGGAGTCCTC 
ACAGTGAGTG ATAGCTGCTT CAGGAATCTT GCAGAAGACC GCAGTGGGAT AAATCTAAAA 
GATCTCGTCC AGGATCCTTC TTTGTTGGGT GGGACTATAT CAGCATACAA GATAGTACCA 
GATGAAATAG AAGAAATCAA GGAAACACTC ATAGACTGGT GTGATGAGAA GGAACTTAAT 
TTGATATTAA CAACTGGCGG AACAGGGTTT GCACCACGAG ATGTCACTCC AGAGGCCACA 
AAAGAAGTAA TAGAACGGGA AGCGCCAGGG ATGGCCCTGG CAATGCTGAT GGGATCACTT 
AATGTTACAC CTCTGGGCAT GCTCTCTAGA CCAGTGTGTG GAATAAGAGG GAAAACTCTG 
ATAATTAACC TGCCTGGTAG CAAGAAAGGA TCTCAGGAAT GCTTTCAGTT CATACTGCCA 
GCTCTACCTC ATGCCATTGA CCTTTTACGT GATGCCATTG TAAAAGTAAA GGAGGTGCAT 
GATGAACTTG AAGATTTACC TTCCCCACCA CCTCCTCTTT CTCCACCTCC TACAACTAGC 
CCACATAAGC AGACAGAAGA CAAAGGAGTT CAGTGTGAAG AGGAGGAAGA AGAAAAGAAA 
GACAGTGGTG TAGCTTCGAC AGAAGATAGT TCCTCATCAC ATATTACTGC AGCAGCTCTT 
GCTGCAAAGA TTCCAGACTC CATCATTTCT CGTGGCGTTC AGGTGCTCCC ACGAGACACA 
GCCTCCCTTA GCACTACTCC TTCAGAGTCG CCCCGTGCCC AGGCTACATC TCGCCTCTCT 
ACAGCTTCTT GTCCAACACC AAAAGTCCAG TCCAGGTGCA GCAGCAAGGA GAACATTCTA 
AGAGCCAGTC ACAGTGCTGT AGATATCACC AAGGTGGCTA GAAGACATCG CATGTCTCCT 
TTTCCTCTGA CGTCTATGGA CAAAGCCTTC ATTACAGTTC TGGAGATGAC TCCGGTGCTT 
GGTACAGAAA TCATCAATTA CCGAGATGGA ATGGGGCGAG TCCTTGCTCA AGATGTGTAT 
GCAAAAGACA ACCTACCCCC ATTCCCAGCA TCAGTAAAAG ATGGCTATGC TGTTCGAGCT 
GCTGATGGTC CAGGAGATCG TTTCATCATT GGGGAATCCC AAGCTGGTGA ACAGCCAACT 
CAGACAGTAA TGCCCGGACA AGTGATGCGG GTTACAACAG GTGCTCCAAT CCCCTGCGGT 
GCTGATGCAG TAGTGCAAGT TGAAGATACT GAACTCATCA GGGAATCAGA TGATGGTACT 
GAAGAACTTG AAGTGCGAAT TCTGGTGCAA GCTCGGCCAG GCCAAGACAT CAGACCCATC 
GGCCACGACA TTAAAAGAGG GGAGTGTGTT TTGGCAAAAG GAACCCATAT GGGCCCTTCA 
GAAATTGGTC TCCTTGCAAC TGTAGGTGTC ACAGAAGTTG AAGTTAATAA GTTTCCAGTG 
GTTGCCGTTA TGTCAACAGG GAATGAGCTA CTAAATCCTG AAGATGACCT CTTACCAGGA 
AAGATTCGGG ACAGCAATCG ATCAACACTT CTAGCAACAA TTCAGGAACA TGGTTACCCA 
ACAATCAACT TGGGAATTGT AGGAGACAAC CCAGATGACT TACTCAATGC CTTGAATGAG 
GGTATCAGCC GTGCTGATGT CATCATCACG TCAGGAGGTG TATCCATGGG GGAAAAGGAC 
TATCTCAAGC AGGTGCTGGA CATCGATCTT CATGCTCAGA TCCATTTTGG CAGGGTTTTT 
ATGAAGCCAG GCTTGCCAAC AACATTTGCA ACTTTGGATA TTGATGGTGT AAGAAAAATT 
ATTTTTGCAC TACCAGGGAA TCCTGTATCA GCTGTGGTCA CCTGCAACCT CTTTGTTGTA 
CCCGCACTGA GAAAAATGCA GGGTATCTTG GATCCTCGGC CAACCATCAT CAAAGCTAGG 
TTATCATGTG ACGTAAAGCT GGACCCTCGC CCAGAATACC ACCGGTGTAT ACTGACTTGG 
CATCACCAAG AACCACTGCC CTGGGCCCAG AGTACAGGTA ATCAGATGAG CAGCCGTCTG 
ATGAGCATGC GCAGTGCCAA TGGATTGCTG ATGCTACCTC CAAAGACAGA GCAGTATGTG 
GAACTTCACA AAGGCGAGGT GGTGGACGTC ATGGTCATCG GACGGCTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_074056.2
CDS	144..2354
Translation	MATEGMILTNHDHQIRVGVLTVSDSCFRNLAEDRSGINLKDLVQDPSLLGGTISAYKIVPDEIEEIKETLIDWCDEKELNLILTTGGTGFAPRDVTPEATKEVIEREAPGMALAMLMGSLNVTPLGMLSRPVCGIRGKTLIINLPGSKKGSQECFQFILPALPHAIDLLRDAIVKVKEVHDELEDLPSPPPPLSPPPTTSPHKQTEDKGVQCEEEEEEKKDSGVASTEDSSSSHITAAALAAKIPDSIISRGVQVLPRDTASLSTTPSESPRAQATSRLSTASCPTPKVQSRCSSKENILRASHSAVDITKVARRHRMSPFPLTSMDKAFITVLEMTPVLGTEIINYRDGMGRVLAQDVYAKDNLPPFPASVKDGYAVRAADGPGDRFIIGESQAGEQPTQTVMPGQVMRVTTGAPIPCGADAVVQVEDTELIRESDDGTEELEVRILVQARPGQDIRPIGHDIKRGECVLAKGTHMGPSEIGLLATVGVTEVEVNKFPVVAVMSTGNELLNPEDDLLPGKIRDSNRSTLLATIQEHGYPTINLGIVGDNPDDLLNALNEGISRADVIITSGGVSMGEKDYLKQVLDIDLHAQIHFGRVFMKPGLPTTFATLDIDGVRKIIFALPGNPVSAVVTCNLFVVPALRKMQGILDPRPTIIKARLSCDVKLDPRPEYHRCILTWHHQEPLPWAQSTGNQMSSRLMSMRSANGLLMLPPKTEQYVELHKGEVVDVMVIGRL

Target ORF information:

RefSeq Version	NM_022865.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus gephyrin (Gphn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022865.3

ORF Insert Sequence:

ATGGCGACCG AGGGAATGAT CCTCACAAAC CACGACCATC AAATCCGTGT CGGAGTCCTC 
ACAGTGAGTG ATAGCTGCTT CAGGAATCTT GCAGAAGACC GCAGTGGGAT AAATCTAAAA 
GATCTCGTCC AGGATCCTTC TTTGTTGGGT GGGACTATAT CAGCATACAA GATAGTACCA 
GATGAAATAG AAGAAATCAA GGAAACACTC ATAGACTGGT GTGATGAGAA GGAACTTAAT 
TTGATATTAA CAACTGGCGG AACAGGGTTT GCACCACGAG ATGTCACTCC AGAGGCCACA 
AAAGAAGTAA TAGAACGGGA AGCGCCAGGG ATGGCCCTGG CAATGCTGAT GGGATCACTT 
AATGTTACAC CTCTGGGCAT GCTCTCTAGA CCAGTGTGTG GAATAAGAGG GAAAACTCTG 
ATAATTAACC TGCCTGGTAG CAAGAAAGGA TCTCAGGAAT GCTTTCAGTT CATACTGCCA 
GCTCTACCTC ATGCCATTGA CCTTTTACGT GATGCCATTG TAAAAGTAAA GGAGGTGCAT 
GATGAACTTG AAGATTTACC TTCCCCACCA CCTCCTCTTT CTCCACCTCC TACAACTAGC 
CCACATAAGC AGACAGAAGA CAAAGGAGTT CAGTGTGAAG AGGAGGAAGA AGAAAAGAAA 
GACAGTGGTG TAGCTTCGAC AGAAGATAGT TCCTCATCAC ATATTACTGC AGCAGCTCTT 
GCTGCAAAGA TTCCAGACTC CATCATTTCT CGTGGCGTTC AGGTGCTCCC ACGAGACACA 
GCCTCCCTTA GCACTACTCC TTCAGAGTCG CCCCGTGCCC AGGCTACATC TCGCCTCTCT 
ACAGCTTCTT GTCCAACACC AAAAGTCCAG TCCAGGTGCA GCAGCAAGGA GAACATTCTA 
AGAGCCAGTC ACAGTGCTGT AGATATCACC AAGGTGGCTA GAAGACATCG CATGTCTCCT 
TTTCCTCTGA CGTCTATGGA CAAAGCCTTC ATTACAGTTC TGGAGATGAC TCCGGTGCTT 
GGTACAGAAA TCATCAATTA CCGAGATGGA ATGGGGCGAG TCCTTGCTCA AGATGTGTAT 
GCAAAAGACA ACCTACCCCC ATTCCCAGCA TCAGTAAAAG ATGGCTATGC TGTTCGAGCT 
GCTGATGGTC CAGGAGATCG TTTCATCATT GGGGAATCCC AAGCTGGTGA ACAGCCAACT 
CAGACAGTAA TGCCCGGACA AGTGATGCGG GTTACAACAG GTGCTCCAAT CCCCTGCGGT 
GCTGATGCAG TAGTGCAAGT TGAAGATACT GAACTCATCA GGGAATCAGA TGATGGTACT 
GAAGAACTTG AAGTGCGAAT TCTGGTGCAA GCTCGGCCAG GCCAAGACAT CAGACCCATC 
GGCCACGACA TTAAAAGAGG GGAGTGTGTT TTGGCAAAAG GAACCCATAT GGGCCCTTCA 
GAAATTGGTC TCCTTGCAAC TGTAGGTGTC ACAGAAGTTG AAGTTAATAA GTTTCCAGTG 
GTTGCCGTTA TGTCAACAGG GAATGAGCTA CTAAATCCTG AAGATGACCT CTTACCAGGA 
AAGATTCGGG ACAGCAATCG ATCAACACTT CTAGCAACAA TTCAGGAACA TGGTTACCCA 
ACAATCAACT TGGGAATTGT AGGAGACAAC CCAGATGACT TACTCAATGC CTTGAATGAG 
GGTATCAGCC GTGCTGATGT CATCATCACG TCAGGAGGTG TATCCATGGG GGAAAAGGAC 
TATCTCAAGC AGGTGCTGGA CATCGATCTT CATGCTCAGA TCCATTTTGG CAGGGTTTTT 
ATGAAGCCAG GCTTGCCAAC AACATTTGCA ACTTTGGATA TTGATGGTGT AAGAAAAATT 
ATTTTTGCAC TACCAGGGAA TCCTGTATCA GCTGTGGTCA CCTGCAACCT CTTTGTTGTA 
CCCGCACTGA GAAAAATGCA GGGTATCTTG GATCCTCGGC CAACCATCAT CAAAGCTAGG 
TTATCATGTG ACGTAAAGCT GGACCCTCGC CCAGAATACC ACCGGTGTAT ACTGACTTGG 
CATCACCAAG AACCACTGCC CTGGGCCCAG AGTACAGGTA ATCAGATGAG CAGCCGTCTG 
ATGAGCATGC GCAGTGCCAA TGGATTGCTG ATGCTACCTC CAAAGACAGA GCAGTATGTG 
GAACTTCACA AAGGCGAGGT GGTGGACGTC ATGGTCATCG GACGGCTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequences Flanking the Gephyrin-Binding Site of GlyRβ Tune Receptor Stabilization at Synapses.
eNeuro5(1)(2018 Jan-Feb)
Grünewald N,Jan A,Salvatico C,Kress V,Renner M,Triller A,Specht CG,Schwarz G

Activity-Dependent Inhibitory Synapse Scaling Is Determined by Gephyrin Phosphorylation and Subsequent Regulation of GABA
eNeuro5(1)(2018 Jan-Feb)
Battaglia S,Renner M,Russeau M,Côme E,Tyagarajan SK,Lévi S

Endosomal Phosphatidylinositol 3-Phosphate Promotes Gephyrin Clustering and GABAergic Neurotransmission at Inhibitory Postsynapses.
The Journal of biological chemistry292(4)1160-1177(2017 01)
Papadopoulos T,Rhee HJ,Subramanian D,Paraskevopoulou F,Mueller R,Schultz C,Brose N,Rhee JS,Betz H

Expression of glycine receptors and gephyrin in rat medial vestibular nuclei and flocculi following unilateral labyrinthectomy.
International journal of molecular medicine38(5)1481-1489(2016 Nov)
Zhou W,Zhou LQ,Shi H,Leng YM,Liu B,Zhang SL,Kong WJ

Identification of an elaborate complex mediating postsynaptic inhibition.
Science (New York, N.Y.)353(6304)1123-9(2016 09)
Uezu A,Kanak DJ,Bradshaw TW,Soderblom EJ,Catavero CM,Burette AC,Weinberg RJ,Soderling SH

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Gphn cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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