Phka1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Phka1
Entrez Gene ID	64561
Full Name	phosphorylase kinase regulatory subunit alpha 1
Synonyms	Pcyt1b
General protein information	Preferred Names phosphorylase kinase regulatory subunit alpha 1 Names phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform PhK-alpha-subunit phosphorylase B kinase alpha subunit phosphorylase kinase alpha M subunit phosphorylase kinase alpha-subunit phosphorylase kinase, alpha 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: X Map Location: Xq22
Summary	catalyzes phosphatidylcholine (PC) synthesis; mediates maintenance of cell membrane PC homeostasis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006257102.3	XP_006257164.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X2
NM_022626.2	NP_072148.2	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform
XM_006257101.3	XP_006257163.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	phka1	XP_005166575.1
Pan troglodytes (chimpanzee)	PHKA1	XP_003317571.2
Mus musculus (house mouse)	Phka1	NP_032858.2
Gallus gallus (chicken)	PHKA1	XP_004940655.1
Macaca mulatta (Rhesus monkey)	PHKA1	XP_001094193.1
Xenopus tropicalis (tropical clawed frog)	phka1	NP_001121510.1
Homo sapiens (human)	PHKA1	NP_002628.2
Canis lupus familiaris (dog)	PHKA1	XP_005641551.1
Rattus norvegicus (Norway rat)	Phka1	NP_072148.1
Danio rerio (zebrafish)	si:dkey-22f5.10	XP_690880.4
Bos taurus (cattle)	PHKA1	XP_005228049.1

Gene Ontology
Bibliography

Related articles in PubMed

Baculovirus-mediated overexpression of the phosphorylase b kinase holoenzyme and alpha gamma delta and gamma delta subcomplexes.
Kumar P, Brushia RJ, Hoye E, Walsh DA
Biochemistry43(31)10247-54(2004 Aug)

Muscle glycogenosis with low phosphorylase kinase activity: mutations in PHKA1, PHKG1 or six other candidate genes explain only a minority of cases.
Burwinkel B, Hu B, Schroers A, Clemens PR, Moses SW, Shin YS, Pongratz D, Vorgerd M, Kilimann MW
European journal of human genetics : EJHG11(7)516-26(2003 Jul)

Both acidic and basic amino acids in an amphitropic enzyme, CTP:phosphocholine cytidylyltransferase, dictate its selectivity for anionic membranes.
Johnson JE, Xie M, Singh LM, Edge R, Cornell RB
The Journal of biological chemistry278(1)514-22(2003 Jan)

The content of glycogen phosphorylase and glycogen in preparations of sarcoplasmic reticulum-glycogenolytic complex is enhanced in diabetic rat skeletal muscle.
Garduño E, Nogues M, Merino JM, Gutiérrez-Merino C, Henao F
Diabetologia44(10)1238-46(2001 Oct)

Phosphorylase kinase: the complexity of its regulation is reflected in the complexity of its structure.
Brushia RJ, Walsh DA
Frontiers in bioscience : a journal and virtual library4D618-41(1999 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Phka1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Phka1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa43608	NM_022626.2 Latest version!	Rattus norvegicus phosphorylase kinase regulatory subunit alpha 1 (Phka1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ORa39807	XM_006257101.3 Latest version!	Rattus norvegicus phosphorylase kinase, alpha 1 (Phka1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ORa39808	XM_006257102.3 Latest version!	Rattus norvegicus phosphorylase kinase, alpha 1 (Phka1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Phka1 ( NM_022626.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_072148.2 Rattus norvegicus phosphorylase kinase regulatory subunit alpha 1 (Phka1), mRNA.

Price & Availability↑

CloneID	ORa43608
Clone ID Related Accession (Same CDS sequence)	NM_022626.2
Accession Version	NM_022626.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577548800000
Organism	Rattus norvegicus(Norway rat)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07039221.1, AABR07039220.1, AABR07039219.1 and AABR07039218.1. On Apr 28, 2015 this sequence version replaced NM_022626.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AF197561.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGGAGCC GGAGTAACTC GGGGGTCCGC CTGGACGGCT ATGCTCGGCT GGTGCATCAG 
ACCATCCTGT GCCATCAGAA TCCAGTGACA GGCTTGCTTC CGGCCAGCTA TGATGAGAAA 
GATGCCTGGG TCCGAGATAA TGTGTATAGC ATCCTGGCTG TGTGGGGTTT AGGCCTGGCA 
TATCGAAAGA ATGCAGACCG TGATGAGGAC AAGGCAAAGG CCTATGAACT GGAGCAGAGT 
GTAGTGAAGT TAATGAGGGG ACTGCTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAG 
TCTTTCAAGT ACAGTCAGAG TACTAAAGAT AGCCTTCACG CCAAGTACAA CACCAAAACT 
TGTGCCACGG TAGTGGGTGA TGACCAGTGG GGACACCTGC AGTTGGATGC TACTTCTGTG 
TACCTGCTCT TCCTAGCACA AATGACTGCC TCAGGACTCC ATATCATCCA CAGCCTAGAC 
GAAGTGAATT TTATACAGAA CCTTGTGTTT TACATTGAAG CCGCATATAA AACTGCTGAC 
TTTGGGATAT GGGAACGCGG AGATAAGACA AACCAAGGCA TCTCAGAATT GAATGCAAGT 
TCAGTTGGAA TGGCCAAGGC AGCCCTGGAA GCACTAGATG AATTAGACTT GTTTGGTGTG 
AAAGGTGGGC CGCAATCAGT TATCCATGTC CTGGCTGACG AAGTACAACA CTGCCAGTCG 
ATCCTGAATT CACTACTGCC CAGGGCTTCA ACATCGAAAG AAGTTGATGC CAGTCTGCTG 
TCAGTGGTCT CTTTCCCAGC CTTTGCTGTA GAGGACAGTC ATTTGGTGGA GATCACCAAA 
CAGGAGATCA TCACCAAACT TCAGGGTCGT TATGGTTGCT GTCGTTTTCT GCGTGATGGA 
TATAAAACTC CTAAAGAGGA TCCTAATCGC CTATACTATG AACCAGCTGA GCTGAAGCTA 
TTTGAAAACA TAGAGTGTGA ATGGCCACTG TTCTGGACAT ACTTTATCCT TGATGGGATC 
TTCAGTGGCA ACACAGAACA GGTTCAAGAA TATAGAGAGG CTCTTGATGC AGTCCTCATC 
AAGGGCAAAA ATGGAGTCCC ACTACTTCCA GAGCTGTACA GTGTTCCTCC TGACAGGGTT 
GATGAAGAAT ATCAAAATCC TCACACCGTG GATCGAGTCC CTATGGGAAA ATTGCCTCAC 
ATGTGGGGTC AGTCCCTATA TATTTTAGGA AACTTGATGG CGGAGGGATT TTTAGCTCCT 
GGAGAAATTG ATCCCCTGAA TCGTAGGTTT TCCACTGTCC CAAAGCCAGA CGTAGTGGTT 
CAAGTCTCCA TTCTGGCTGA AACAGAAGAA ATCAAGGCCA TTTTGAAGGA CAAAGGAATT 
GATGTGGAGA CCATTGCTGA AGTGTACCCC ATAAGAGTAC AGCCAGCTCG TATTCTCAGC 
CACATTTATT CCAGTCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGACG ACCCTACAGG 
CTCATGGGTG TACTTGGAAC GTCAAAACTT TATGACATTC GCAAAACTAT CTTTACTTTC 
ACTCCACAGT TTATAGACCA GCAGCAGTTC TACTTGGCTC TGGACAACCA GATGATAGTC 
GAAATGCTCC GGACAGATCT CTCCTACCTC TGCAGCCGGT GGAGGATGAC AGGCCAGCCC 
ACCATCACTT TCCCCATCTC ACACACCATG CTGGATGAAG ATGGAGCCAG CTTGAATTCA 
AGTATCCTGG CAGCACTCCG GAAAATGCAG GATGGTTATT TTGGTGGGGC CAGGATCCAA 
ACAGGTAAGC TGTCAGAGTT TTTGACAACA TCTTGCTGCA CACACTTAAG CTTCATGGAC 
CCTGGACCCG AGGGTAAGCT GTACAGCGAA GATTATGATG AAGACTATGA TGATGAGCTG 
GACTCTGGCA ACTGGATGGA TAGCTATGAC TCAACACGTA ATGCTCGCTG TGGTGATGAA 
GTTGCCCGTT ATTTAGACCA CCTTTTGGCA CACACTGGTC CCCATCCTAA ACTAACTCCC 
ACCTCACGGA AGGGAGGGCT AGATCGGTTC CGAGCTGCTG TGCAAACAAC TTGCGACTTA 
ATGTCCTTGG TGGCCAAGGC CAAGGAGCTG CATATACAGA ATGTTCACAT GTATCTTCCT 
ACAAAGTTAT TTCAGCCTTC TCGCCCTTCA CTCAACTTAC TTGACTCACC TGAATCTCCA 
CAAGATAGCC AGGTTCCTTC TGTTCGTGTA GAAGTCCATC TTCCTAGGGA CCAGTCTGGG 
GAAGTGGACT TCCAGTCATT GGTTTCACAG TTGAAGGAGA CCTCAAGCTT ACAGGAGCAA 
GCTGATATAC TCTACATGCT ATATACAATG AAAGGACCTG ACTGGAACAC TGAATTGTAC 
GAAGAAGGAG GCAGTACTGT GAGAGAGCTT CTTAGTGAAC TATATGTCAA AGTCGGTGAA 
ATTCGGCACT GGGGTCTGAT CCGATACATC TCTGGGATCT TACGGAAGAA GGTGGAGGCA 
CTTGATGAGG CCTGCACAGA CCTTCTGTCC TACCAGAAAC ACCTGACAGT AGGGCTTCCT 
CCAGAACCTC GAGAGAAGAC CATCTCTGCG CCTCTACCGT ACGAGGCACT CACTAAGCTG 
ATAGATGAAG CCAGCGAAGG AGACATGAAC ATCTCCACCC TGACACAGGA AATAATGGTC 
TATCTTGCCA TGTATATGAG AACTCAGCCT GGCCTTTTTG CAGAAATGTT CCGACTTCGA 
ATTGGTTTGA TCATACAAGT TATGGCAACA GAACTGGCCC ACTCTCTGCG ATGTTCAGCT 
GAAGAAGCCA CAGAGGGCCT GATGAATCTC AGTCCTTCAG CCATGAAGAA TCTCCTGCAT 
CACATCCTCA GCGGCAAAGA GTTTGGAGTG GAACGAAGCG TTCGTCCCAC TGATTCAAAT 
GTGAGTCCTG CTATTTCCAT CCATGAGATC GGTGCTGTCG GAGCAACAAA AACAGAACGA 
ACTGGAATCA TGCAGTTAAA AAGTGAGATC AAGCAGGTGG AATTCCGTAG GCTGTCTGTC 
TCACCCGAGA GTCAGACTAG TGGTGGTCAT CCCTCAAGTA TAGATTTGAT GTCACCGACC 
TTTCTGTCCC CTGCAGCCTG TATTTCTGCG AGCAGTGGGT CCTTTCCTAC TGTATGTGAG 
CCACAGACAT CTAAAGATAG TCGTCAAGGC CAATGGCAAC GCAGGAGAAG ACTAGATGGA 
GCACTAAATA GAGTACCAAT TGGATTTTAT CAAAAAGTGT GGAAAATTTT GCAGAAATGT 
CACGGGCTTT CTGTGGAAGG TTTTGTTCTT CCCTCTTCAA GCACTAGGGA GATGACCCCA 
GGCGAGATTA AATTCTCTGT CCACGTGGAG TCCGTCCTGA ACCGTGTGCC TCAGCCAGAG 
TACCGTCAGC TTCTGGTTGA AGCCATCCTC GTTCTCACCA TGCTGGCAGA TATTGAAATC 
CATAGCATTG GGAGCATCAT TGCTGTGGAA AAAATAGTTC ATATTGCCAA TGACTTGTTC 
CTTCAGGAAC AGAAAACCCT CGGCGCAGAT GATATCATGT TGGCAAAGGA TCCTGCATCT 
GGCATCTGTA CTCTTCTGTA TGACAGTGCA CCCAGTGGCA GATTTGGCAC CATGACCTAC 
CTCTCCAAGG CAGCTGCCAC CTACGTGCAG GAGTTTCTGC CACACAGCCT CTGTGCCATG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_072148.2
CDS	1..3726
Translation	MRSRSNSGVRLDGYARLVHQTILCHQNPVTGLLPASYDEKDAWVRDNVYSILAVWGLGLAYRKNADRDEDKAKAYELEQSVVKLMRGLLHCMIRQVDKVESFKYSQSTKDSLHAKYNTKTCATVVGDDQWGHLQLDATSVYLLFLAQMTASGLHIIHSLDEVNFIQNLVFYIEAAYKTADFGIWERGDKTNQGISELNASSVGMAKAALEALDELDLFGVKGGPQSVIHVLADEVQHCQSILNSLLPRASTSKEVDASLLSVVSFPAFAVEDSHLVEITKQEIITKLQGRYGCCRFLRDGYKTPKEDPNRLYYEPAELKLFENIECEWPLFWTYFILDGIFSGNTEQVQEYREALDAVLIKGKNGVPLLPELYSVPPDRVDEEYQNPHTVDRVPMGKLPHMWGQSLYILGNLMAEGFLAPGEIDPLNRRFSTVPKPDVVVQVSILAETEEIKAILKDKGIDVETIAEVYPIRVQPARILSHIYSSLGCNSRMKLSGRPYRLMGVLGTSKLYDIRKTIFTFTPQFIDQQQFYLALDNQMIVEMLRTDLSYLCSRWRMTGQPTITFPISHTMLDEDGASLNSSILAALRKMQDGYFGGARIQTGKLSEFLTTSCCTHLSFMDPGPEGKLYSEDYDEDYDDELDSGNWMDSYDSTRNARCGDEVARYLDHLLAHTGPHPKLTPTSRKGGLDRFRAAVQTTCDLMSLVAKAKELHIQNVHMYLPTKLFQPSRPSLNLLDSPESPQDSQVPSVRVEVHLPRDQSGEVDFQSLVSQLKETSSLQEQADILYMLYTMKGPDWNTELYEEGGSTVRELLSELYVKVGEIRHWGLIRYISGILRKKVEALDEACTDLLSYQKHLTVGLPPEPREKTISAPLPYEALTKLIDEASEGDMNISTLTQEIMVYLAMYMRTQPGLFAEMFRLRIGLIIQVMATELAHSLRCSAEEATEGLMNLSPSAMKNLLHHILSGKEFGVERSVRPTDSNVSPAISIHEIGAVGATKTERTGIMQLKSEIKQVEFRRLSVSPESQTSGGHPSSIDLMSPTFLSPAACISASSGSFPTVCEPQTSKDSRQGQWQRRRRLDGALNRVPIGFYQKVWKILQKCHGLSVEGFVLPSSSTREMTPGEIKFSVHVESVLNRVPQPEYRQLLVEAILVLTMLADIEIHSIGSIIAVEKIVHIANDLFLQEQKTLGADDIMLAKDPASGICTLLYDSAPSGRFGTMTYLSKAAATYVQEFLPHSLCAMQ

Target ORF information:

RefSeq Version	NM_022626.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphorylase kinase regulatory subunit alpha 1 (Phka1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022626.2

ORF Insert Sequence:

ATGAGGAGCC GGAGTAACTC GGGGGTCCGC CTGGACGGCT ATGCTCGGCT GGTGCATCAG 
ACCATCCTGT GCCATCAGAA TCCAGTGACA GGCTTGCTTC CGGCCAGCTA TGATGAGAAA 
GATGCCTGGG TCCGAGATAA TGTGTATAGC ATCCTGGCTG TGTGGGGTTT AGGCCTGGCA 
TATCGAAAGA ATGCAGACCG TGATGAGGAC AAGGCAAAGG CCTATGAACT GGAGCAGAGT 
GTAGTGAAGT TAATGAGGGG ACTGCTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAG 
TCTTTCAAGT ACAGTCAGAG TACTAAAGAT AGCCTTCACG CCAAGTACAA CACCAAAACT 
TGTGCCACGG TAGTGGGTGA TGACCAGTGG GGACACCTGC AGTTGGATGC TACTTCTGTG 
TACCTGCTCT TCCTAGCACA AATGACTGCC TCAGGACTCC ATATCATCCA CAGCCTAGAC 
GAAGTGAATT TTATACAGAA CCTTGTGTTT TACATTGAAG CCGCATATAA AACTGCTGAC 
TTTGGGATAT GGGAACGCGG AGATAAGACA AACCAAGGCA TCTCAGAATT GAATGCAAGT 
TCAGTTGGAA TGGCCAAGGC AGCCCTGGAA GCACTAGATG AATTAGACTT GTTTGGTGTG 
AAAGGTGGGC CGCAATCAGT TATCCATGTC CTGGCTGACG AAGTACAACA CTGCCAGTCG 
ATCCTGAATT CACTACTGCC CAGGGCTTCA ACATCGAAAG AAGTTGATGC CAGTCTGCTG 
TCAGTGGTCT CTTTCCCAGC CTTTGCTGTA GAGGACAGTC ATTTGGTGGA GATCACCAAA 
CAGGAGATCA TCACCAAACT TCAGGGTCGT TATGGTTGCT GTCGTTTTCT GCGTGATGGA 
TATAAAACTC CTAAAGAGGA TCCTAATCGC CTATACTATG AACCAGCTGA GCTGAAGCTA 
TTTGAAAACA TAGAGTGTGA ATGGCCACTG TTCTGGACAT ACTTTATCCT TGATGGGATC 
TTCAGTGGCA ACACAGAACA GGTTCAAGAA TATAGAGAGG CTCTTGATGC AGTCCTCATC 
AAGGGCAAAA ATGGAGTCCC ACTACTTCCA GAGCTGTACA GTGTTCCTCC TGACAGGGTT 
GATGAAGAAT ATCAAAATCC TCACACCGTG GATCGAGTCC CTATGGGAAA ATTGCCTCAC 
ATGTGGGGTC AGTCCCTATA TATTTTAGGA AACTTGATGG CGGAGGGATT TTTAGCTCCT 
GGAGAAATTG ATCCCCTGAA TCGTAGGTTT TCCACTGTCC CAAAGCCAGA CGTAGTGGTT 
CAAGTCTCCA TTCTGGCTGA AACAGAAGAA ATCAAGGCCA TTTTGAAGGA CAAAGGAATT 
GATGTGGAGA CCATTGCTGA AGTGTACCCC ATAAGAGTAC AGCCAGCTCG TATTCTCAGC 
CACATTTATT CCAGTCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGACG ACCCTACAGG 
CTCATGGGTG TACTTGGAAC GTCAAAACTT TATGACATTC GCAAAACTAT CTTTACTTTC 
ACTCCACAGT TTATAGACCA GCAGCAGTTC TACTTGGCTC TGGACAACCA GATGATAGTC 
GAAATGCTCC GGACAGATCT CTCCTACCTC TGCAGCCGGT GGAGGATGAC AGGCCAGCCC 
ACCATCACTT TCCCCATCTC ACACACCATG CTGGATGAAG ATGGAGCCAG CTTGAATTCA 
AGTATCCTGG CAGCACTCCG GAAAATGCAG GATGGTTATT TTGGTGGGGC CAGGATCCAA 
ACAGGTAAGC TGTCAGAGTT TTTGACAACA TCTTGCTGCA CACACTTAAG CTTCATGGAC 
CCTGGACCCG AGGGTAAGCT GTACAGCGAA GATTATGATG AAGACTATGA TGATGAGCTG 
GACTCTGGCA ACTGGATGGA TAGCTATGAC TCAACACGTA ATGCTCGCTG TGGTGATGAA 
GTTGCCCGTT ATTTAGACCA CCTTTTGGCA CACACTGGTC CCCATCCTAA ACTAACTCCC 
ACCTCACGGA AGGGAGGGCT AGATCGGTTC CGAGCTGCTG TGCAAACAAC TTGCGACTTA 
ATGTCCTTGG TGGCCAAGGC CAAGGAGCTG CATATACAGA ATGTTCACAT GTATCTTCCT 
ACAAAGTTAT TTCAGCCTTC TCGCCCTTCA CTCAACTTAC TTGACTCACC TGAATCTCCA 
CAAGATAGCC AGGTTCCTTC TGTTCGTGTA GAAGTCCATC TTCCTAGGGA CCAGTCTGGG 
GAAGTGGACT TCCAGTCATT GGTTTCACAG TTGAAGGAGA CCTCAAGCTT ACAGGAGCAA 
GCTGATATAC TCTACATGCT ATATACAATG AAAGGACCTG ACTGGAACAC TGAATTGTAC 
GAAGAAGGAG GCAGTACTGT GAGAGAGCTT CTTAGTGAAC TATATGTCAA AGTCGGTGAA 
ATTCGGCACT GGGGTCTGAT CCGATACATC TCTGGGATCT TACGGAAGAA GGTGGAGGCA 
CTTGATGAGG CCTGCACAGA CCTTCTGTCC TACCAGAAAC ACCTGACAGT AGGGCTTCCT 
CCAGAACCTC GAGAGAAGAC CATCTCTGCG CCTCTACCGT ACGAGGCACT CACTAAGCTG 
ATAGATGAAG CCAGCGAAGG AGACATGAAC ATCTCCACCC TGACACAGGA AATAATGGTC 
TATCTTGCCA TGTATATGAG AACTCAGCCT GGCCTTTTTG CAGAAATGTT CCGACTTCGA 
ATTGGTTTGA TCATACAAGT TATGGCAACA GAACTGGCCC ACTCTCTGCG ATGTTCAGCT 
GAAGAAGCCA CAGAGGGCCT GATGAATCTC AGTCCTTCAG CCATGAAGAA TCTCCTGCAT 
CACATCCTCA GCGGCAAAGA GTTTGGAGTG GAACGAAGCG TTCGTCCCAC TGATTCAAAT 
GTGAGTCCTG CTATTTCCAT CCATGAGATC GGTGCTGTCG GAGCAACAAA AACAGAACGA 
ACTGGAATCA TGCAGTTAAA AAGTGAGATC AAGCAGGTGG AATTCCGTAG GCTGTCTGTC 
TCACCCGAGA GTCAGACTAG TGGTGGTCAT CCCTCAAGTA TAGATTTGAT GTCACCGACC 
TTTCTGTCCC CTGCAGCCTG TATTTCTGCG AGCAGTGGGT CCTTTCCTAC TGTATGTGAG 
CCACAGACAT CTAAAGATAG TCGTCAAGGC CAATGGCAAC GCAGGAGAAG ACTAGATGGA 
GCACTAAATA GAGTACCAAT TGGATTTTAT CAAAAAGTGT GGAAAATTTT GCAGAAATGT 
CACGGGCTTT CTGTGGAAGG TTTTGTTCTT CCCTCTTCAA GCACTAGGGA GATGACCCCA 
GGCGAGATTA AATTCTCTGT CCACGTGGAG TCCGTCCTGA ACCGTGTGCC TCAGCCAGAG 
TACCGTCAGC TTCTGGTTGA AGCCATCCTC GTTCTCACCA TGCTGGCAGA TATTGAAATC 
CATAGCATTG GGAGCATCAT TGCTGTGGAA AAAATAGTTC ATATTGCCAA TGACTTGTTC 
CTTCAGGAAC AGAAAACCCT CGGCGCAGAT GATATCATGT TGGCAAAGGA TCCTGCATCT 
GGCATCTGTA CTCTTCTGTA TGACAGTGCA CCCAGTGGCA GATTTGGCAC CATGACCTAC 
CTCTCCAAGG CAGCTGCCAC CTACGTGCAG GAGTTTCTGC CACACAGCCT CTGTGCCATG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phka1 ( XM_006257101.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006257163.1 Rattus norvegicus phosphorylase kinase, alpha 1 (Phka1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa39807
Clone ID Related Accession (Same CDS sequence)	XM_006257101.3
Accession Version	XM_006257101.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3687bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006257101.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCC GGAGTAACTC GGGGGTCCGC CTGGACGGCT ATGCTCGGCT GGTGCATCAG 
ACCATCCTGT GCCATCAGAA TCCAGTGACA GGCTTGCTTC CGGCCAGCTA TGATGAGAAA 
GATGCCTGGG TCCGAGATAA TGTGTATAGC ATCCTGGCTG TGTGGGGTTT AGGCCTGGCA 
TATCGAAAGA ATGCAGACCG TGATGAGGAC AAGGCAAAGG CCTATGAACT GGAGCAGAGT 
GTAGTGAAGT TAATGAGGGG ACTGCTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAG 
TCTTTCAAGT ACAGTCAGAG TACTAAAGAT AGCCTTCACG CCAAGTACAA CACCAAAACT 
TGTGCCACGG TAGTGGGTGA TGACCAGTGG GGACACCTGC AGTTGGATGC TACTTCTGTG 
TACCTGCTCT TCCTAGCACA AATGACTGCC TCAGGACTCC ATATCATCCA CAGCCTAGAC 
GAAGTGAATT TTATACAGAA CCTTGTGTTT TACATTGAAG CCGCATATAA AACTGCTGAC 
TTTGGGATAT GGGAACGCGG AGATAAGACA AACCAAGGCA TCTCAGAATT GAATGCAAGT 
TCAGTTGGAA TGGCCAAGGC AGCCCTGGAA GCACTAGATG AATTAGACTT GTTTGGTGTG 
AAAGGTGGGC CGCAATCAGT TATCCATGTC CTGGCTGACG AAGTACAACA CTGCCAGTCG 
ATCCTGAATT CACTACTGCC CAGGGCTTCA ACATCGAAAG AAGTTGATGC CAGTCTGCTG 
TCAGTGGTCT CTTTCCCAGC CTTTGCTGTA GAGGACAGTC ATTTGGTGGA GATCACCAAA 
CAGGAGATCA TCACCAAACT TCAGGGTCGT TATGGTTGCT GTCGTTTTCT GCGTGATGGA 
TATAAAACTC CTAAAGAGGA TCCTAATCGC CTATACTATG AACCAGCTGA GCTGAAGCTA 
TTTGAAAACA TAGAGTGTGA ATGGCCACTG TTCTGGACAT ACTTTATCCT TGATGGGATC 
TTCAGTGGCA ACACAGAACA GGTTCAAGAA TATAGAGAGG CTCTTGATGC AGTCCTCATC 
AAGGGCAAAA ATGGAGTCCC ACTACTTCCA GAGCTGTACA GTGTTCCTCC TGACAGGGTT 
GATGAAGAAT ATCAAAATCC TCACACCGTG GATCGAGTCC CTATGGGAAA ATTGCCTCAC 
ATGTGGGGTC AGTCCCTATA TATTTTAGGA AACTTGATGG CGGAGGGATT TTTAGCTCCT 
GGAGAAATTG ATCCCCTGAA TCGTAGGTTT TCCACTGTCC CAAAGCCAGA CGTAGTGGTT 
CAAGTCTCCA TTCTGGCTGA AACAGAAGAA ATCAAGGCCA TTTTGAAGGA CAAAGGAATT 
GATGTGGAGA CCATTGCTGA AGTGTACCCC ATAAGAGTAC AGCCAGCTCG TATTCTCAGC 
CACATTTATT CCAGTCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGACG ACCCTACAGG 
CTCATGGGTG TACTTGGAAC GTCAAAACTT TATGACATTC GCAAAACTAT CTTTACTTTC 
ACTCCACAGT TTATAGACCA GCAGCAGTTC TACTTGGCTC TGGACAACCA GATGATAGTC 
GAAATGCTCC GGACAGATCT CTCCTACCTC TGCAGCCGGT GGAGGATGAC AGGCCAGCCC 
ACCATCACTT TCCCCATCTC ACACACCATG CTGGATGAAG ATGGAGCCAG CTTGAATTCA 
AGTATCCTGG CAGCACTCCG GAAAATGCAG GATGGTTATT TTGGTGGGGC CAGGATCCAA 
ACAGGTAAGC TGTCAGAGTT TTTGACAACA TCTTGCTGCA CACACTTAAG CTTCATGGAC 
CCTGGACCCG AGGGTAAGCT GTACAGCGAA GATTATGATG AAGACTATGA TGATGAGCTG 
GACTCTGGCA ACTGGATGGA TAGCTATGAC TCAACACGTA ATGCTCGCTG TGGTGATGAA 
GTTGCCCGTT ATTTAGACCA CCTTTTGGCA CACACTGGTC CCCATCCTAA ACTAACTCCC 
ACCTCACGGA AGGGAGGGCT AGATCGGTTC CGAGCTGCTG TGCAAACAAC TTGCGACTTA 
ATGTCCTTGG TGGCCAAGGC CAAGGAGCTG CATATACAGA ATGTTCACAT GTATCTTCCT 
ACAAAGTTAT TTCAGCCTTC TCGCCCTTCA CTCAACTTAC TTGACTCACC TGAATCTCCA 
CAAGATAGCC AGGTTCCTTC TGTTCGTGTA GAAGTCCATC TTCCTAGGGA CCAGTCTGGG 
GAAGTGGACT TCCAGTCATT GGTTTCACAG TTGAAGGAGA CCTCAAGCTT ACAGGAGCAA 
GCTGATATAC TCTACATGCT ATATACAATG AAAGGACCTG ACTGGAACAC TGAATTGTAC 
GAAGAAGGAG GCAGTACTGT GAGAGAGCTT CTTAGTGAAC TATATGTCAA AGTCGGTGAA 
ATTCGGCACT GGGGTCTGAT CCGATACATC TCTGGGATCT TACGGAAGAA GGTGGAGGCA 
CTTGATGAGG CCTGCACAGA CCTTCTGTCC TACCAGAAAC ACCTGACAGT AGGGCTTCCT 
CCAGAACCTC GAGAGAAGAC CATCTCTGCG CCTCTACCGT ACGAGGCACT CACTAAGCTG 
ATAGATGAAG CCAGCGAAGG AGACATGAAC ATCTCCACCC TGACACAGGA AATAATGGTC 
TATCTTGCCA TGTATATGAG AACTCAGCCT GGCCTTTTTG CAGAAATGTT CCGACTTCGA 
ATTGGTTTGA TCATACAAGT TATGGCAACA GAACTGGCCC ACTCTCTGCG ATGTTCAGCT 
GAAGAAGCCA CAGAGGGCCT GATGAATCTC AGTCCTTCAG CCATGAAGAA TCTCCTGCAT 
CACATCCTCA GCGGCAAAGA GTTTGGAGTG GAACGAAGCG TTCGTCCCAC TGATTCAAAT 
GTGAGTCCTG CTATTTCCAT CCATGAGATC GGTGCTGTCG GAGCAACAAA AACAGAACGA 
ACTGGAATCA TGCAGTTAAA AAGTGAGATC AAGCAGACTA GTGGTGGTCA TCCCTCAAGT 
ATAGATTTGA TGTCACCGAC CTTTCTGTCC CCTGCAGCCT GTATTTCTGC GAGCAGTGGG 
TCCTTTCCTA CTGTATGTGA GCCACAGACA TCTAAAGATA GTCGTCAAGG CCAATGGCAA 
CGCAGGAGAA GACTAGATGG AGCACTAAAT AGAGTACCAA TTGGATTTTA TCAAAAAGTG 
TGGAAAATTT TGCAGAAATG TCACGGGCTT TCTGTGGAAG GTTTTGTTCT TCCCTCTTCA 
AGCACTAGGG AGATGACCCC AGGCGAGATT AAATTCTCTG TCCACGTGGA GTCCGTCCTG 
AACCGTGTGC CTCAGCCAGA GTACCGTCAG CTTCTGGTTG AAGCCATCCT CGTTCTCACC 
ATGCTGGCAG ATATTGAAAT CCATAGCATT GGGAGCATCA TTGCTGTGGA AAAAATAGTT 
CATATTGCCA ATGACTTGTT CCTTCAGGAA CAGAAAACCC TCGGCGCAGA TGATATCATG 
TTGGCAAAGG ATCCTGCATC TGGCATCTGT ACTCTTCTGT ATGACAGTGC ACCCAGTGGC 
AGATTTGGCA CCATGACCTA CCTCTCCAAG GCAGCTGCCA CCTACGTGCA GGAGTTTCTG 
CCACACAGCC TCTGTGCCAT GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006257163.1
CDS	329..4015
Translation	MRSRSNSGVRLDGYARLVHQTILCHQNPVTGLLPASYDEKDAWVRDNVYSILAVWGLGLAYRKNADRDEDKAKAYELEQSVVKLMRGLLHCMIRQVDKVESFKYSQSTKDSLHAKYNTKTCATVVGDDQWGHLQLDATSVYLLFLAQMTASGLHIIHSLDEVNFIQNLVFYIEAAYKTADFGIWERGDKTNQGISELNASSVGMAKAALEALDELDLFGVKGGPQSVIHVLADEVQHCQSILNSLLPRASTSKEVDASLLSVVSFPAFAVEDSHLVEITKQEIITKLQGRYGCCRFLRDGYKTPKEDPNRLYYEPAELKLFENIECEWPLFWTYFILDGIFSGNTEQVQEYREALDAVLIKGKNGVPLLPELYSVPPDRVDEEYQNPHTVDRVPMGKLPHMWGQSLYILGNLMAEGFLAPGEIDPLNRRFSTVPKPDVVVQVSILAETEEIKAILKDKGIDVETIAEVYPIRVQPARILSHIYSSLGCNSRMKLSGRPYRLMGVLGTSKLYDIRKTIFTFTPQFIDQQQFYLALDNQMIVEMLRTDLSYLCSRWRMTGQPTITFPISHTMLDEDGASLNSSILAALRKMQDGYFGGARIQTGKLSEFLTTSCCTHLSFMDPGPEGKLYSEDYDEDYDDELDSGNWMDSYDSTRNARCGDEVARYLDHLLAHTGPHPKLTPTSRKGGLDRFRAAVQTTCDLMSLVAKAKELHIQNVHMYLPTKLFQPSRPSLNLLDSPESPQDSQVPSVRVEVHLPRDQSGEVDFQSLVSQLKETSSLQEQADILYMLYTMKGPDWNTELYEEGGSTVRELLSELYVKVGEIRHWGLIRYISGILRKKVEALDEACTDLLSYQKHLTVGLPPEPREKTISAPLPYEALTKLIDEASEGDMNISTLTQEIMVYLAMYMRTQPGLFAEMFRLRIGLIIQVMATELAHSLRCSAEEATEGLMNLSPSAMKNLLHHILSGKEFGVERSVRPTDSNVSPAISIHEIGAVGATKTERTGIMQLKSEIKQTSGGHPSSIDLMSPTFLSPAACISASSGSFPTVCEPQTSKDSRQGQWQRRRRLDGALNRVPIGFYQKVWKILQKCHGLSVEGFVLPSSSTREMTPGEIKFSVHVESVLNRVPQPEYRQLLVEAILVLTMLADIEIHSIGSIIAVEKIVHIANDLFLQEQKTLGADDIMLAKDPASGICTLLYDSAPSGRFGTMTYLSKAAATYVQEFLPHSLCAMQ

Target ORF information:

RefSeq Version	XM_006257101.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphorylase kinase, alpha 1 (Phka1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006257101.3

ORF Insert Sequence:

ATGAGGAGCC GGAGTAACTC GGGGGTCCGC CTGGACGGCT ATGCTCGGCT GGTGCATCAG 
ACCATCCTGT GCCATCAGAA TCCAGTGACA GGCTTGCTTC CGGCCAGCTA TGATGAGAAA 
GATGCCTGGG TCCGAGATAA TGTGTATAGC ATCCTGGCTG TGTGGGGTTT AGGCCTGGCA 
TATCGAAAGA ATGCAGACCG TGATGAGGAC AAGGCAAAGG CCTATGAACT GGAGCAGAGT 
GTAGTGAAGT TAATGAGGGG ACTGCTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAG 
TCTTTCAAGT ACAGTCAGAG TACTAAAGAT AGCCTTCACG CCAAGTACAA CACCAAAACT 
TGTGCCACGG TAGTGGGTGA TGACCAGTGG GGACACCTGC AGTTGGATGC TACTTCTGTG 
TACCTGCTCT TCCTAGCACA AATGACTGCC TCAGGACTCC ATATCATCCA CAGCCTAGAC 
GAAGTGAATT TTATACAGAA CCTTGTGTTT TACATTGAAG CCGCATATAA AACTGCTGAC 
TTTGGGATAT GGGAACGCGG AGATAAGACA AACCAAGGCA TCTCAGAATT GAATGCAAGT 
TCAGTTGGAA TGGCCAAGGC AGCCCTGGAA GCACTAGATG AATTAGACTT GTTTGGTGTG 
AAAGGTGGGC CGCAATCAGT TATCCATGTC CTGGCTGACG AAGTACAACA CTGCCAGTCG 
ATCCTGAATT CACTACTGCC CAGGGCTTCA ACATCGAAAG AAGTTGATGC CAGTCTGCTG 
TCAGTGGTCT CTTTCCCAGC CTTTGCTGTA GAGGACAGTC ATTTGGTGGA GATCACCAAA 
CAGGAGATCA TCACCAAACT TCAGGGTCGT TATGGTTGCT GTCGTTTTCT GCGTGATGGA 
TATAAAACTC CTAAAGAGGA TCCTAATCGC CTATACTATG AACCAGCTGA GCTGAAGCTA 
TTTGAAAACA TAGAGTGTGA ATGGCCACTG TTCTGGACAT ACTTTATCCT TGATGGGATC 
TTCAGTGGCA ACACAGAACA GGTTCAAGAA TATAGAGAGG CTCTTGATGC AGTCCTCATC 
AAGGGCAAAA ATGGAGTCCC ACTACTTCCA GAGCTGTACA GTGTTCCTCC TGACAGGGTT 
GATGAAGAAT ATCAAAATCC TCACACCGTG GATCGAGTCC CTATGGGAAA ATTGCCTCAC 
ATGTGGGGTC AGTCCCTATA TATTTTAGGA AACTTGATGG CGGAGGGATT TTTAGCTCCT 
GGAGAAATTG ATCCCCTGAA TCGTAGGTTT TCCACTGTCC CAAAGCCAGA CGTAGTGGTT 
CAAGTCTCCA TTCTGGCTGA AACAGAAGAA ATCAAGGCCA TTTTGAAGGA CAAAGGAATT 
GATGTGGAGA CCATTGCTGA AGTGTACCCC ATAAGAGTAC AGCCAGCTCG TATTCTCAGC 
CACATTTATT CCAGTCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGACG ACCCTACAGG 
CTCATGGGTG TACTTGGAAC GTCAAAACTT TATGACATTC GCAAAACTAT CTTTACTTTC 
ACTCCACAGT TTATAGACCA GCAGCAGTTC TACTTGGCTC TGGACAACCA GATGATAGTC 
GAAATGCTCC GGACAGATCT CTCCTACCTC TGCAGCCGGT GGAGGATGAC AGGCCAGCCC 
ACCATCACTT TCCCCATCTC ACACACCATG CTGGATGAAG ATGGAGCCAG CTTGAATTCA 
AGTATCCTGG CAGCACTCCG GAAAATGCAG GATGGTTATT TTGGTGGGGC CAGGATCCAA 
ACAGGTAAGC TGTCAGAGTT TTTGACAACA TCTTGCTGCA CACACTTAAG CTTCATGGAC 
CCTGGACCCG AGGGTAAGCT GTACAGCGAA GATTATGATG AAGACTATGA TGATGAGCTG 
GACTCTGGCA ACTGGATGGA TAGCTATGAC TCAACACGTA ATGCTCGCTG TGGTGATGAA 
GTTGCCCGTT ATTTAGACCA CCTTTTGGCA CACACTGGTC CCCATCCTAA ACTAACTCCC 
ACCTCACGGA AGGGAGGGCT AGATCGGTTC CGAGCTGCTG TGCAAACAAC TTGCGACTTA 
ATGTCCTTGG TGGCCAAGGC CAAGGAGCTG CATATACAGA ATGTTCACAT GTATCTTCCT 
ACAAAGTTAT TTCAGCCTTC TCGCCCTTCA CTCAACTTAC TTGACTCACC TGAATCTCCA 
CAAGATAGCC AGGTTCCTTC TGTTCGTGTA GAAGTCCATC TTCCTAGGGA CCAGTCTGGG 
GAAGTGGACT TCCAGTCATT GGTTTCACAG TTGAAGGAGA CCTCAAGCTT ACAGGAGCAA 
GCTGATATAC TCTACATGCT ATATACAATG AAAGGACCTG ACTGGAACAC TGAATTGTAC 
GAAGAAGGAG GCAGTACTGT GAGAGAGCTT CTTAGTGAAC TATATGTCAA AGTCGGTGAA 
ATTCGGCACT GGGGTCTGAT CCGATACATC TCTGGGATCT TACGGAAGAA GGTGGAGGCA 
CTTGATGAGG CCTGCACAGA CCTTCTGTCC TACCAGAAAC ACCTGACAGT AGGGCTTCCT 
CCAGAACCTC GAGAGAAGAC CATCTCTGCG CCTCTACCGT ACGAGGCACT CACTAAGCTG 
ATAGATGAAG CCAGCGAAGG AGACATGAAC ATCTCCACCC TGACACAGGA AATAATGGTC 
TATCTTGCCA TGTATATGAG AACTCAGCCT GGCCTTTTTG CAGAAATGTT CCGACTTCGA 
ATTGGTTTGA TCATACAAGT TATGGCAACA GAACTGGCCC ACTCTCTGCG ATGTTCAGCT 
GAAGAAGCCA CAGAGGGCCT GATGAATCTC AGTCCTTCAG CCATGAAGAA TCTCCTGCAT 
CACATCCTCA GCGGCAAAGA GTTTGGAGTG GAACGAAGCG TTCGTCCCAC TGATTCAAAT 
GTGAGTCCTG CTATTTCCAT CCATGAGATC GGTGCTGTCG GAGCAACAAA AACAGAACGA 
ACTGGAATCA TGCAGTTAAA AAGTGAGATC AAGCAGACTA GTGGTGGTCA TCCCTCAAGT 
ATAGATTTGA TGTCACCGAC CTTTCTGTCC CCTGCAGCCT GTATTTCTGC GAGCAGTGGG 
TCCTTTCCTA CTGTATGTGA GCCACAGACA TCTAAAGATA GTCGTCAAGG CCAATGGCAA 
CGCAGGAGAA GACTAGATGG AGCACTAAAT AGAGTACCAA TTGGATTTTA TCAAAAAGTG 
TGGAAAATTT TGCAGAAATG TCACGGGCTT TCTGTGGAAG GTTTTGTTCT TCCCTCTTCA 
AGCACTAGGG AGATGACCCC AGGCGAGATT AAATTCTCTG TCCACGTGGA GTCCGTCCTG 
AACCGTGTGC CTCAGCCAGA GTACCGTCAG CTTCTGGTTG AAGCCATCCT CGTTCTCACC 
ATGCTGGCAG ATATTGAAAT CCATAGCATT GGGAGCATCA TTGCTGTGGA AAAAATAGTT 
CATATTGCCA ATGACTTGTT CCTTCAGGAA CAGAAAACCC TCGGCGCAGA TGATATCATG 
TTGGCAAAGG ATCCTGCATC TGGCATCTGT ACTCTTCTGT ATGACAGTGC ACCCAGTGGC 
AGATTTGGCA CCATGACCTA CCTCTCCAAG GCAGCTGCCA CCTACGTGCA GGAGTTTCTG 
CCACACAGCC TCTGTGCCAT GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phka1 ( XM_006257102.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006257164.1 Rattus norvegicus phosphorylase kinase, alpha 1 (Phka1), transcript variant X2, mRNA.

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CloneID	ORa39808
Clone ID Related Accession (Same CDS sequence)	XM_006257102.3
Accession Version	XM_006257102.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006257102.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCC GGAGTAACTC GGGGGTCCGC CTGGACGGCT ATGCTCGGCT GGTGCATCAG 
ACCATCCTGT GCCATCAGAA TCCAGTGACA GGCTTGCTTC CGGCCAGCTA TGATGAGAAA 
GATGCCTGGG TCCGAGATAA TGTGTATAGC ATCCTGGCTG TGTGGGGTTT AGGCCTGGCA 
TATCGAAAGA ATGCAGACCG TGATGAGGAC AAGGCAAAGG CCTATGAACT GGAGCAGAGT 
GTAGTGAAGT TAATGAGGGG ACTGCTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAG 
TCTTTCAAGT ACAGTCAGAG TACTAAAGAT AGCCTTCACG CCAAGTACAA CACCAAAACT 
TGTGCCACGG TAGTGGGTGA TGACCAGTGG GGACACCTGC AGTTGGATGC TACTTCTGTG 
TACCTGCTCT TCCTAGCACA AATGACTGCC TCAGGACTCC ATATCATCCA CAGCCTAGAC 
GAAGTGAATT TTATACAGAA CCTTGTGTTT TACATTGAAG CCGCATATAA AACTGCTGAC 
TTTGGGATAT GGGAACGCGG AGATAAGACA AACCAAGGCA TCTCAGAATT GAATGCAAGT 
TCAGTTGGAA TGGCCAAGGC AGCCCTGGAA GCACTAGATG AATTAGACTT GTTTGGTGTG 
AAAGGTGGGC CGCAATCAGT TATCCATGTC CTGGCTGACG AAGTACAACA CTGCCAGTCG 
ATCCTGAATT CACTACTGCC CAGGGCTTCA ACATCGAAAG AAGTTGATGC CAGTCTGCTG 
TCAGTGGTCT CTTTCCCAGC CTTTGCTGTA GAGGACAGTC ATTTGGTGGA GATCACCAAA 
CAGGAGATCA TCACCAAACT TCAGGGTCGT TATGGTTGCT GTCGTTTTCT GCGTGATGGA 
TATAAAACTC CTAAAGAGGA TCCTAATCGC CTATACTATG AACCAGCTGA GCTGAAGCTA 
TTTGAAAACA TAGAGTGTGA ATGGCCACTG TTCTGGACAT ACTTTATCCT TGATGGGATC 
TTCAGTGGCA ACACAGAACA GGTTCAAGAA TATAGAGAGG CTCTTGATGC AGTCCTCATC 
AAGGGCAAAA ATGGAGTCCC ACTACTTCCA GAGCTGTACA GTGTTCCTCC TGACAGGGTT 
GATGAAGAAT ATCAAAATCC TCACACCGTG GATCGAGTCC CTATGGGAAA ATTGCCTCAC 
ATGTGGGGTC AGTCCCTATA TATTTTAGGA AACTTGATGG CGGAGGGATT TTTAGCTCCT 
GGAGAAATTG ATCCCCTGAA TCGTAGGTTT TCCACTGTCC CAAAGCCAGA CGTAGTGGTT 
CAAGTCTCCA TTCTGGCTGA AACAGAAGAA ATCAAGGCCA TTTTGAAGGA CAAAGGAATT 
GATGTGGAGA CCATTGCTGA AGTGTACCCC ATAAGAGTAC AGCCAGCTCG TATTCTCAGC 
CACATTTATT CCAGTCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGACG ACCCTACAGG 
CTCATGGGTG TACTTGGAAC GTCAAAACTT TATGACATTC GCAAAACTAT CTTTACTTTC 
ACTCCACAGT TTATAGACCA GCAGCAGTTC TACTTGGCTC TGGACAACCA GATGATAGTC 
GAAATGCTCC GGACAGATCT CTCCTACCTC TGCAGCCGGT GGAGGATGAC AGGCCAGCCC 
ACCATCACTT TCCCCATCTC ACACACCATG CTGGATGAAG ATGGAGCCAG CTTGAATTCA 
AGTATCCTGG CAGCACTCCG GAAAATGCAG GATGGTTATT TTGGTGGGGC CAGGATCCAA 
ACAGGTAAGC TGTCAGAGTT TTTGACAACA TCTTGCTGCA CACACTTAAG CTTCATGGAC 
CCTGGACCCG AGGGTAAGCT GTACAGCGAA GATTATGATG AAGACTATGA TGATGAGCTG 
GACTCTGGCA ACTGGATGGA TAGCTATGAC TCAACACGTA ATGATGTTCA CATGTATCTT 
CCTACAAAGT TATTTCAGCC TTCTCGCCCT TCACTCAACT TACTTGACTC ACCTGAATCT 
CCACAAGATA GCCAGGTTCC TTCTGTTCGT GTAGAAGTCC ATCTTCCTAG GGACCAGTCT 
GGGGAAGTGG ACTTCCAGTC ATTGGTTTCA CAGTTGAAGG AGACCTCAAG CTTACAGGAG 
CAAGCTGATA TACTCTACAT GCTATATACA ATGAAAGGAC CTGACTGGAA CACTGAATTG 
TACGAAGAAG GAGGCAGTAC TGTGAGAGAG CTTCTTAGTG AACTATATGT CAAAGTCGGT 
GAAATTCGGC ACTGGGGTCT GATCCGATAC ATCTCTGGGA TCTTACGGAA GAAGGTGGAG 
GCACTTGATG AGGCCTGCAC AGACCTTCTG TCCTACCAGA AACACCTGAC AGTAGGGCTT 
CCTCCAGAAC CTCGAGAGAA GACCATCTCT GCGCCTCTAC CGTACGAGGC ACTCACTAAG 
CTGATAGATG AAGCCAGCGA AGGAGACATG AACATCTCCA CCCTGACACA GGAAATAATG 
GTCTATCTTG CCATGTATAT GAGAACTCAG CCTGGCCTTT TTGCAGAAAT GTTCCGACTT 
CGAATTGGTT TGATCATACA AGTTATGGCA ACAGAACTGG CCCACTCTCT GCGATGTTCA 
GCTGAAGAAG CCACAGAGGG CCTGATGAAT CTCAGTCCTT CAGCCATGAA GAATCTCCTG 
CATCACATCC TCAGCGGCAA AGAGTTTGGA GTGGAACGAA GCGTTCGTCC CACTGATTCA 
AATGTGAGTC CTGCTATTTC CATCCATGAG ATCGGTGCTG TCGGAGCAAC AAAAACAGAA 
CGAACTGGAA TCATGCAGTT AAAAAGTGAG ATCAAGCAGG TGGAATTCCG TAGGCTGTCT 
GTCTCACCCG AGAGTCAGAC TAGTGGTGGT CATCCCTCAA GTATAGATTT GATGTCACCG 
ACCTTTCTGT CCCCTGCAGC CTGTATTTCT GCGAGCAGTG GGTCCTTTCC TACTGTATGT 
GAGCCACAGA CATCTAAAGA TAGTCGTCAA GGCCAATGGC AACGCAGGAG AAGACTAGAT 
GGAGCACTAA ATAGAGTACC AATTGGATTT TATCAAAAAG TGTGGAAAAT TTTGCAGAAA 
TGTCACGGGC TTTCTGTGGA AGGTTTTGTT CTTCCCTCTT CAAGCACTAG GGAGATGACC 
CCAGGCGAGA TTAAATTCTC TGTCCACGTG GAGTCCGTCC TGAACCGTGT GCCTCAGCCA 
GAGTACCGTC AGCTTCTGGT TGAAGCCATC CTCGTTCTCA CCATGCTGGC AGATATTGAA 
ATCCATAGCA TTGGGAGCAT CATTGCTGTG GAAAAAATAG TTCATATTGC CAATGACTTG 
TTCCTTCAGG AACAGAAAAC CCTCGGCGCA GATGATATCA TGTTGGCAAA GGATCCTGCA 
TCTGGCATCT GTACTCTTCT GTATGACAGT GCACCCAGTG GCAGATTTGG CACCATGACC 
TACCTCTCCA AGGCAGCTGC CACCTACGTG CAGGAGTTTC TGCCACACAG CCTCTGTGCC 
ATGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006257164.1
CDS	329..3877
Translation	MRSRSNSGVRLDGYARLVHQTILCHQNPVTGLLPASYDEKDAWVRDNVYSILAVWGLGLAYRKNADRDEDKAKAYELEQSVVKLMRGLLHCMIRQVDKVESFKYSQSTKDSLHAKYNTKTCATVVGDDQWGHLQLDATSVYLLFLAQMTASGLHIIHSLDEVNFIQNLVFYIEAAYKTADFGIWERGDKTNQGISELNASSVGMAKAALEALDELDLFGVKGGPQSVIHVLADEVQHCQSILNSLLPRASTSKEVDASLLSVVSFPAFAVEDSHLVEITKQEIITKLQGRYGCCRFLRDGYKTPKEDPNRLYYEPAELKLFENIECEWPLFWTYFILDGIFSGNTEQVQEYREALDAVLIKGKNGVPLLPELYSVPPDRVDEEYQNPHTVDRVPMGKLPHMWGQSLYILGNLMAEGFLAPGEIDPLNRRFSTVPKPDVVVQVSILAETEEIKAILKDKGIDVETIAEVYPIRVQPARILSHIYSSLGCNSRMKLSGRPYRLMGVLGTSKLYDIRKTIFTFTPQFIDQQQFYLALDNQMIVEMLRTDLSYLCSRWRMTGQPTITFPISHTMLDEDGASLNSSILAALRKMQDGYFGGARIQTGKLSEFLTTSCCTHLSFMDPGPEGKLYSEDYDEDYDDELDSGNWMDSYDSTRNDVHMYLPTKLFQPSRPSLNLLDSPESPQDSQVPSVRVEVHLPRDQSGEVDFQSLVSQLKETSSLQEQADILYMLYTMKGPDWNTELYEEGGSTVRELLSELYVKVGEIRHWGLIRYISGILRKKVEALDEACTDLLSYQKHLTVGLPPEPREKTISAPLPYEALTKLIDEASEGDMNISTLTQEIMVYLAMYMRTQPGLFAEMFRLRIGLIIQVMATELAHSLRCSAEEATEGLMNLSPSAMKNLLHHILSGKEFGVERSVRPTDSNVSPAISIHEIGAVGATKTERTGIMQLKSEIKQVEFRRLSVSPESQTSGGHPSSIDLMSPTFLSPAACISASSGSFPTVCEPQTSKDSRQGQWQRRRRLDGALNRVPIGFYQKVWKILQKCHGLSVEGFVLPSSSTREMTPGEIKFSVHVESVLNRVPQPEYRQLLVEAILVLTMLADIEIHSIGSIIAVEKIVHIANDLFLQEQKTLGADDIMLAKDPASGICTLLYDSAPSGRFGTMTYLSKAAATYVQEFLPHSLCAMQ

Target ORF information:

RefSeq Version	XM_006257102.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphorylase kinase, alpha 1 (Phka1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006257102.3

ORF Insert Sequence:

ATGAGGAGCC GGAGTAACTC GGGGGTCCGC CTGGACGGCT ATGCTCGGCT GGTGCATCAG 
ACCATCCTGT GCCATCAGAA TCCAGTGACA GGCTTGCTTC CGGCCAGCTA TGATGAGAAA 
GATGCCTGGG TCCGAGATAA TGTGTATAGC ATCCTGGCTG TGTGGGGTTT AGGCCTGGCA 
TATCGAAAGA ATGCAGACCG TGATGAGGAC AAGGCAAAGG CCTATGAACT GGAGCAGAGT 
GTAGTGAAGT TAATGAGGGG ACTGCTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAG 
TCTTTCAAGT ACAGTCAGAG TACTAAAGAT AGCCTTCACG CCAAGTACAA CACCAAAACT 
TGTGCCACGG TAGTGGGTGA TGACCAGTGG GGACACCTGC AGTTGGATGC TACTTCTGTG 
TACCTGCTCT TCCTAGCACA AATGACTGCC TCAGGACTCC ATATCATCCA CAGCCTAGAC 
GAAGTGAATT TTATACAGAA CCTTGTGTTT TACATTGAAG CCGCATATAA AACTGCTGAC 
TTTGGGATAT GGGAACGCGG AGATAAGACA AACCAAGGCA TCTCAGAATT GAATGCAAGT 
TCAGTTGGAA TGGCCAAGGC AGCCCTGGAA GCACTAGATG AATTAGACTT GTTTGGTGTG 
AAAGGTGGGC CGCAATCAGT TATCCATGTC CTGGCTGACG AAGTACAACA CTGCCAGTCG 
ATCCTGAATT CACTACTGCC CAGGGCTTCA ACATCGAAAG AAGTTGATGC CAGTCTGCTG 
TCAGTGGTCT CTTTCCCAGC CTTTGCTGTA GAGGACAGTC ATTTGGTGGA GATCACCAAA 
CAGGAGATCA TCACCAAACT TCAGGGTCGT TATGGTTGCT GTCGTTTTCT GCGTGATGGA 
TATAAAACTC CTAAAGAGGA TCCTAATCGC CTATACTATG AACCAGCTGA GCTGAAGCTA 
TTTGAAAACA TAGAGTGTGA ATGGCCACTG TTCTGGACAT ACTTTATCCT TGATGGGATC 
TTCAGTGGCA ACACAGAACA GGTTCAAGAA TATAGAGAGG CTCTTGATGC AGTCCTCATC 
AAGGGCAAAA ATGGAGTCCC ACTACTTCCA GAGCTGTACA GTGTTCCTCC TGACAGGGTT 
GATGAAGAAT ATCAAAATCC TCACACCGTG GATCGAGTCC CTATGGGAAA ATTGCCTCAC 
ATGTGGGGTC AGTCCCTATA TATTTTAGGA AACTTGATGG CGGAGGGATT TTTAGCTCCT 
GGAGAAATTG ATCCCCTGAA TCGTAGGTTT TCCACTGTCC CAAAGCCAGA CGTAGTGGTT 
CAAGTCTCCA TTCTGGCTGA AACAGAAGAA ATCAAGGCCA TTTTGAAGGA CAAAGGAATT 
GATGTGGAGA CCATTGCTGA AGTGTACCCC ATAAGAGTAC AGCCAGCTCG TATTCTCAGC 
CACATTTATT CCAGTCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGACG ACCCTACAGG 
CTCATGGGTG TACTTGGAAC GTCAAAACTT TATGACATTC GCAAAACTAT CTTTACTTTC 
ACTCCACAGT TTATAGACCA GCAGCAGTTC TACTTGGCTC TGGACAACCA GATGATAGTC 
GAAATGCTCC GGACAGATCT CTCCTACCTC TGCAGCCGGT GGAGGATGAC AGGCCAGCCC 
ACCATCACTT TCCCCATCTC ACACACCATG CTGGATGAAG ATGGAGCCAG CTTGAATTCA 
AGTATCCTGG CAGCACTCCG GAAAATGCAG GATGGTTATT TTGGTGGGGC CAGGATCCAA 
ACAGGTAAGC TGTCAGAGTT TTTGACAACA TCTTGCTGCA CACACTTAAG CTTCATGGAC 
CCTGGACCCG AGGGTAAGCT GTACAGCGAA GATTATGATG AAGACTATGA TGATGAGCTG 
GACTCTGGCA ACTGGATGGA TAGCTATGAC TCAACACGTA ATGATGTTCA CATGTATCTT 
CCTACAAAGT TATTTCAGCC TTCTCGCCCT TCACTCAACT TACTTGACTC ACCTGAATCT 
CCACAAGATA GCCAGGTTCC TTCTGTTCGT GTAGAAGTCC ATCTTCCTAG GGACCAGTCT 
GGGGAAGTGG ACTTCCAGTC ATTGGTTTCA CAGTTGAAGG AGACCTCAAG CTTACAGGAG 
CAAGCTGATA TACTCTACAT GCTATATACA ATGAAAGGAC CTGACTGGAA CACTGAATTG 
TACGAAGAAG GAGGCAGTAC TGTGAGAGAG CTTCTTAGTG AACTATATGT CAAAGTCGGT 
GAAATTCGGC ACTGGGGTCT GATCCGATAC ATCTCTGGGA TCTTACGGAA GAAGGTGGAG 
GCACTTGATG AGGCCTGCAC AGACCTTCTG TCCTACCAGA AACACCTGAC AGTAGGGCTT 
CCTCCAGAAC CTCGAGAGAA GACCATCTCT GCGCCTCTAC CGTACGAGGC ACTCACTAAG 
CTGATAGATG AAGCCAGCGA AGGAGACATG AACATCTCCA CCCTGACACA GGAAATAATG 
GTCTATCTTG CCATGTATAT GAGAACTCAG CCTGGCCTTT TTGCAGAAAT GTTCCGACTT 
CGAATTGGTT TGATCATACA AGTTATGGCA ACAGAACTGG CCCACTCTCT GCGATGTTCA 
GCTGAAGAAG CCACAGAGGG CCTGATGAAT CTCAGTCCTT CAGCCATGAA GAATCTCCTG 
CATCACATCC TCAGCGGCAA AGAGTTTGGA GTGGAACGAA GCGTTCGTCC CACTGATTCA 
AATGTGAGTC CTGCTATTTC CATCCATGAG ATCGGTGCTG TCGGAGCAAC AAAAACAGAA 
CGAACTGGAA TCATGCAGTT AAAAAGTGAG ATCAAGCAGG TGGAATTCCG TAGGCTGTCT 
GTCTCACCCG AGAGTCAGAC TAGTGGTGGT CATCCCTCAA GTATAGATTT GATGTCACCG 
ACCTTTCTGT CCCCTGCAGC CTGTATTTCT GCGAGCAGTG GGTCCTTTCC TACTGTATGT 
GAGCCACAGA CATCTAAAGA TAGTCGTCAA GGCCAATGGC AACGCAGGAG AAGACTAGAT 
GGAGCACTAA ATAGAGTACC AATTGGATTT TATCAAAAAG TGTGGAAAAT TTTGCAGAAA 
TGTCACGGGC TTTCTGTGGA AGGTTTTGTT CTTCCCTCTT CAAGCACTAG GGAGATGACC 
CCAGGCGAGA TTAAATTCTC TGTCCACGTG GAGTCCGTCC TGAACCGTGT GCCTCAGCCA 
GAGTACCGTC AGCTTCTGGT TGAAGCCATC CTCGTTCTCA CCATGCTGGC AGATATTGAA 
ATCCATAGCA TTGGGAGCAT CATTGCTGTG GAAAAAATAG TTCATATTGC CAATGACTTG 
TTCCTTCAGG AACAGAAAAC CCTCGGCGCA GATGATATCA TGTTGGCAAA GGATCCTGCA 
TCTGGCATCT GTACTCTTCT GTATGACAGT GCACCCAGTG GCAGATTTGG CACCATGACC 
TACCTCTCCA AGGCAGCTGC CACCTACGTG CAGGAGTTTC TGCCACACAG CCTCTGTGCC 
ATGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Baculovirus-mediated overexpression of the phosphorylase b kinase holoenzyme and alpha gamma delta and gamma delta subcomplexes.
Biochemistry43(31)10247-54(2004 Aug)
Kumar P,Brushia RJ,Hoye E,Walsh DA

Muscle glycogenosis with low phosphorylase kinase activity: mutations in PHKA1, PHKG1 or six other candidate genes explain only a minority of cases.
European journal of human genetics : EJHG11(7)516-26(2003 Jul)
Burwinkel B,Hu B,Schroers A,Clemens PR,Moses SW,Shin YS,Pongratz D,Vorgerd M,Kilimann MW

Both acidic and basic amino acids in an amphitropic enzyme, CTP:phosphocholine cytidylyltransferase, dictate its selectivity for anionic membranes.
The Journal of biological chemistry278(1)514-22(2003 Jan)
Johnson JE,Xie M,Singh LM,Edge R,Cornell RB

The content of glycogen phosphorylase and glycogen in preparations of sarcoplasmic reticulum-glycogenolytic complex is enhanced in diabetic rat skeletal muscle.
Diabetologia44(10)1238-46(2001 Oct)
Garduño E,Nogues M,Merino JM,Gutiérrez-Merino C,Henao F

Phosphorylase kinase: the complexity of its regulation is reflected in the complexity of its structure.
Frontiers in bioscience : a journal and virtual library4D618-41(1999 Sep)
Brushia RJ,Walsh DA

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Phka1 cDNA ORF clone, Rattus norvegicus(Norway rat)

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